title: Does aging affect the immune status? A comparative analysis in 300 healthy volunteers from France, Austria and Spain.
abstract: BACKGROUND
  As the European population is getting older, there is growing need in scientific data on how to achieve healthy and successful aging. A decline in immune function with age is unanimously supported by many epidemiological and clinical observations, with a decrease in T-cell mediated function encompassing a large part of this alteration. In the EU-funded VITAGE project, the effects of aging on biomarkers of immune status are being studied in three European countries. According to strict inclusion/exclusion criteria, a cohort of 300 healthy male non-smoking 20-75 years old volunteers were enrolled in France (n = 99), Spain (n = 100) and Austria (n = 101). In each country, the volunteers were classified as a function of age (one age group per decade). Biomarkers of immune status were determined including delayed-type hypersensitivity tests, measurement of lymphocyte surface markers, and serum determinations of interleukin-2, complement fractions and immunoglobulins.
  
  
  RESULTS
  There were moderate differences in the biomarkers of immune status of the VITAGE study volunteers among the three European centres. The percentage of Natural Killer (NK) cells was 156% and 142% higher in Spain as compared to France and Austria, respectively (p < 0.0001), and this increase was observed at any age group above 30 years. Comparison between age-groups showed that in Spain, but not in France or Austria, older individuals had significantly a lower B lymphocyte distribution and conversely, a higher NK cell distribution. Moreover, the CD4/CD8 ratio was positively correlated with age in Austrian subjects (p < 0.0001).
  
  
  CONCLUSIONS
  Our results provide evidence of an increased NK cell distribution in the elderly, especially in the Spanish population. NK cell status may predict morbidity and mortality in the elderly, emphasizing the importance of innate as well as adaptive immunity in ensuring healthy longevity and cancer resistance, possibly in link with the Mediterranean diet.
authors.affiliation: Clermont Université, Université d'Auvergne, Unité de Nutrition Humaine, F-63000, Clermont-Ferrand, France. m-paule.vasson@udamail.fr.
authors.forename: Marie-Paule
authors.initials: MP
authors.lastname: Vasson
authors.forename: Marie-Chantal
authors.initials: MC
authors.lastname: Farges
authors.forename: Nicolas
authors.initials: N
authors.lastname: Goncalves-Mendes
authors.forename: Jérémie
authors.initials: J
authors.lastname: Talvas
authors.forename: Josep
authors.initials: J
authors.lastname: Ribalta
authors.forename: Brigitte
authors.initials: B
authors.lastname: Winklhofer-Roob
authors.forename: Edmond
authors.initials: E
authors.lastname: Rock
authors.forename: Adrien
authors.initials: A
authors.lastname: Rossary