title: Expression, purification and crystallization of the ancestral androgen receptor-DHT complex.
abstract: Steroid receptors (SRs) are a closely related family of ligand-dependent nuclear receptors that mediate the transcription of genes critical for development, reproduction and immunity. SR dysregulation has been implicated in cancer, inflammatory diseases and metabolic disorders. SRs bind their cognate hormone ligand with exquisite specificity, offering a unique system to study the evolution of molecular recognition. The SR family evolved from an estrogen-sensitive ancestor and diverged to become sensitive to progestagens, corticoids and, most recently, androgens. To understand the structural mechanisms driving the evolution of androgen responsiveness, the ancestral androgen receptor (ancAR1) was crystallized in complex with 5α-dihydrotestosterone (DHT) and a fragment of the transcriptional mediator/intermediary factor 2 (Tif2). Crystals diffracted to 2.1 Å resolution and the resulting structure will permit a direct comparison with its progestagen-sensitive ancestor, ancestral steroid receptor 2 (AncSR2).
authors.affiliation: Department of Biochemistry and Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA 30033, USA.
authors.forename: Jennifer K
authors.initials: JK
authors.lastname: Colucci
authors.forename: Eric A
authors.initials: EA
authors.lastname: Ortlund
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D018360:Crystallography, X-Ray; D013196:Dihydrotestosterone / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D019143:Evolution, Molecular; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D051958:Nuclear Receptor Coactivator 2 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D017433:Protein Structure, Secondary; D011944:Receptors, Androgen / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: C410798:AR protein, human; C498001:NCOA2 protein, human; D051958:Nuclear Receptor Coactivator 2; D011944:Receptors, Androgen; D011994:Recombinant Proteins; D013196:Dihydrotestosterone
keywords: 5α-dihydrotestosterone; ancestral androgen receptor; steroid receptors