title: Separation of steroid isomers by ion mobility mass spectrometry.
abstract: Ion mobility mass spectrometry performed in a compact traveling wave cell (TWIM-MS) is shown to provide a reliable, fast and repeatable method to separate derivatized steroid isomers. Three steroid isomer pairs were analyzed in their native form and as their p-toluenesulfonyl isocyanate derivatives. The native steroids were separated from each other, but no separation could be attained for the isomers. The derivatized steroid isomers were, however, properly separated by TWIM-MS with peak-to-peak resolutions close to or as high as baseline resolution (Rp-p=0.77-1.08).
authors.affiliation: Division of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, FI-00014, Finland. Electronic address: linda.ahonen@helsinki.fi.
authors.forename: Linda
authors.initials: L
authors.lastname: Ahonen
authors.forename: Maíra
authors.initials: M
authors.lastname: Fasciotti
authors.forename: Gustav Boije Af
authors.initials: GB
authors.lastname: Gennäs
authors.forename: Tapio
authors.initials: T
authors.lastname: Kotiaho
authors.forename: Romeu J
authors.initials: RJ
authors.lastname: Daroda
authors.forename: Marcos
authors.initials: M
authors.lastname: Eberlin
authors.forename: Risto
authors.initials: R
authors.lastname: Kostiainen
mesh_terms: D007536:Isomerism; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D013256:Steroids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D013256:Steroids
keywords: Collision cross section; Derivatization; Ion mobility mass spectrometry; Steroid isomers; Steroids