title: Structural characterization and antitumor activity of a pectic polysaccharide from Codonopsis pilosula.
abstract: A pectic polysaccharide (CPP1b) was at first isolated from Codonopsis pilosula. Sugar analysis revealed that CPP1b is composed of rhamnose (Rha), arabinose (Ara), galactose (Gal) and galacturonic acid (GalA) with a molar ratio of 0.25:0.12:0.13:2.51. The result of esterification assay showed that about 46.7±0.4% of carboxylic groups in GalA residues existed as methyl ester. Combined with chemical and spectroscopic analyses, a preliminary structure of CPP1b was proposed as follows: 1,4-linked α-D-GalpA and 1,4-linked α-D-GalpA6Me interspersed with rare 1,2-linked β-L-Rhap, 1,2,6-linked α-D-Galp and terminal α-L-Arap. CPP1b had an average molar mass and root-mean square radius (RMS) of 1.45×10(5) Da and 29.7 nm, respectively, and presented a linear random coil conformation in 0.9% NaCl. The ultrastructure of CPP1b was further investigated by transmission electron microscope (TEM) and scanning electron microscope (SEM). CPP1b exhibited obvious cytotoxicity to human lung adenocarcinoma A549 cells in a dose- and time-dependent manner.
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Yang
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Gou
authors.forename: Jiayu
authors.initials: J
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: An
authors.forename: Wenxia
authors.initials: W
authors.lastname: Chen
authors.forename: Fangdi
authors.initials: F
authors.lastname: Hu
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D029745:Codonopsis / Q000737:chemistry*; D004951:Esterification; D006801:Humans; D008745:Methylation; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D010368:Pectins
keywords: Codonopsis pilosula; Cytotoxicity; GPC-MALLS; NMR; Pectic polysaccharide; Ultrastructure