title: Combined effects of carbonation with heating and fatty acid esters on inactivation and growth inhibition of various bacillus spores.
abstract: The effects of carbonation treatment (1 to 5 MPa, 30 min) plus heat treatment (30 to 80°C, 30 min) in the presence of various fatty acid esters (FAEs; 0.05 and 0.1%, wt/vol) on counts of viable Bacillus subtilis spores were investigated. FAEs or carbonation alone had no inactivation or growth inhibition effects on B. subtilis spores. However, carbonation plus heat (CH; 80°C, 5 MPa, 30 min) in the presence of mono- and diglycerol fatty acid esters markedly decreased counts of viable spores, and the spore counts did not change during storage for 30 days. The greatest decrease in viable spore counts occurred in the presence of monoglycerol fatty acid esters. Under CH conditions, inactivation and/or growth inhibition occurred at only 80°C and increased with increasing pressure. The greatest decrease in spore counts (more than 4 log units) occurred with CH (80°C, 5 MPa, 30 min) in the presence of monoglycerol fatty acid esters. However, this treatment was less effective against Bacillus coagulans and Geobacillus stearothermophilus spores.
authors.affiliation: Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Wannaporn
authors.initials: W
authors.lastname: Klangpetch
authors.forename: Tomoe
authors.initials: T
authors.lastname: Nakai
authors.forename: Seiji
authors.initials: S
authors.lastname: Noma
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Igura
authors.forename: Mitsuya
authors.initials: M
authors.lastname: Shimoda
mesh_terms: D001412:Bacillus subtilis / Q000254:growth & development / Q000502:physiology*; D002255:Carbonic Acid / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004952:Esters / Q000494:pharmacology; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D006358:Hot Temperature; D006874:Hydrostatic Pressure; D013171:Spores, Bacterial / Q000187:drug effects / Q000254:growth & development
chemical_list: D004952:Esters; D002255:Carbonic Acid