title: Protective effect of compounds from the flowers of Citrus aurantium L. var. amara Engl against carbon tetrachloride-induced hepatocyte injury.
abstract: 5-Hydroxy-6,7,3',4'-tetramethoxyflavone (HTF), limonexic acid (LA) are two compounds isolated from the flowers of Citrus aurantium L. var. amara Engl with various biological activities. This study was designed to investigate their protective effects against carbon tetrachloride (CCl4)-induced hepatocyte injury, using human hepatic cell line HL-7702 to determine the cell cytotoxicity, cell viability, levels of hepatic marker enzymes, malondialdehyde (MDA). Results showed that pretreatment with HTF, LA could significantly reverse CCl4-induced HL-7702 cell viability decrease, LA displayed a higher activity. HTF, LA also showed their capability of decreasing the CCl4-induced leakage of lactate dehydrogenase (LDH), aspartate aminotransferase (AST), inhibiting the lipid peroxidation, HTF showed more significant activity. Given that HTF, LA were not toxic, it is concluded that HTF, LA could effectively protect hepatocyte against CCl4-induced injury.
authors.affiliation: College of Food and Bioengineering, South China University of Technology, Guangzhou 510640, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Lu
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Zhao
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Jiang
authors.forename: Xi-Lin
authors.initials: XL
authors.lastname: Xu
mesh_terms: D001219:Aspartate Aminotransferases / Q000378:metabolism; D002251:Carbon Tetrachloride / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D002957:Citrus / Q000737:chemistry*; D047309:Flavones / Q000494:pharmacology; D035264:Flowers / Q000737:chemistry; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D036701:Limonins / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*
chemical_list: C000590666:5-hydroxy-6,7,3',4'-tetramethoxyflavone; D047309:Flavones; D036701:Limonins; D020011:Protective Agents; C525538:limonexic acid; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases
keywords: 5-Hydroxy-6,7,3′,4′-tetramethoxyflavone; Carbon tetrachloride; Citrus aurantium L. var. amara Engl; Hepatocyte injury; Limonexic acid