title: Nuclear patch-clamp electrophysiology of Ca2+ channels.
abstract: Patch-clamping the outer or inner nuclear membrane of isolated nuclei is very similar to patch-clamping the plasma membrane of isolated cells. This protocol describes in detail all the steps required to successfully obtain nuclear membrane patches, in various configurations, from both the outer and inner nuclear membranes of isolated nuclei.
authors.affiliation: Department of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
authors.forename: Don-On Daniel
authors.initials: DO
authors.lastname: Mak
authors.forename: Horia
authors.initials: H
authors.lastname: Vais
authors.forename: King-Ho
authors.initials: KH
authors.lastname: Cheung
authors.forename: J Kevin
authors.initials: JK
authors.lastname: Foskett
mesh_terms: D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000502:physiology*; D002413:Cations, Divalent / Q000378:metabolism; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000379:methods*; D009685:Nuclear Envelope / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000379:methods*
chemical_list: D015220:Calcium Channels; D002413:Cations, Divalent; D002118:Calcium