title: A technique for the prevention of greenhouse whitefly (Trialeurodes vaporariorum) using the entomopathogenic fungus Beauveria bassiana M130.
abstract: The possibility of using hyphomycete fungi as suitable biocontrol agents against greenhouse whitefly has led to the isolation of various insect pathogenic fungi. Among them is Beauveria bassiana, one of the most studied entomopathogenic fungi. The objective of this study was to use B. bassiana M130 as an insecticidal agent against the greenhouse whitefly. M130 isolated from infected insects is known to be a biocontrol agent against greenhouse whitefly. Phylogenetic classification of M130 was determined according to its morphological features and 18S rRNA sequence analysis. M130 was identified as B. bassiana M130 and showed chitinase (342.28 units/ml) and protease (461.70 units/ml) activities, which were involved in the invasion of the host through the outer cuticle layer, thus killing them. The insecticidal activity was 55.2% in petri-dish test, 84.6% in pot test, and 45.3% in field test. The results of this study indicate that B. bassiana has potential as a biological agent for the control of greenhouse whitefly to replace chemical pesticides.
authors.affiliation: Department of Bioresource Sciences, Andong National University, Andong 760-749, Republic of Korea.
authors.forename: Chang-Su
authors.initials: CS
authors.lastname: Kim
authors.forename: Jung-Bok
authors.initials: JB
authors.lastname: Lee
authors.forename: Beam-Soo
authors.initials: BS
authors.lastname: Kim
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Nam
authors.forename: Kee-Sun
authors.initials: KS
authors.lastname: Shin
authors.forename: Jin-Won
authors.initials: JW
authors.lastname: Kim
authors.forename: Jang-Eok
authors.initials: JE
authors.lastname: Kim
authors.forename: Gi-Seok
authors.initials: GS
authors.lastname: Kwon
mesh_terms: D000818:Animals; D052982:Beauveria / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D006430:Hemiptera / Q000382:microbiology* / Q000502:physiology*; D010572:Pest Control, Biological / Q000379:methods*; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D016019:Survival Analysis
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012331:RNA, Fungal; D012337:RNA, Ribosomal, 18S