title: The effect of titanium with heparin/BMP-2 complex for improving osteoblast activity.
abstract: The objective of this study was to investigate the enhanced osteoblast activity of MG-63 cells cultured on titanium (Ti) with a heparin/BMP-2 (Hep/BMP-2) complex. The Ti substrates were initially modified by chemical grafting poly-L-lysine (PLL) using condensing agent, followed by immobilizing the heparin/BMP-2 complex to the PLL-grafted Ti substrate via electrostatic interactions. The surface modification of Ti substrates with PLL and/or Hep/BMP-2 complex were confirmed with scanning electron microscopy, contact angle measurements, and X-ray photoelectron spectroscopy. Immobilized BMP-2 was released from the Hep/BMP-2/Ti substrate in a sustained manner. In vitro studies revealed that osteoblasts grown on Hep/BMP-2/Ti substrate increased ALP activity, calcium deposition, ALP and osteocalcin levels as compared to those grown on pristine Ti or PLL-Ti. These results indicated that heparin/BMP-2 complex immobilized Ti substrate can be useful to effectively improve osteoblast activity.
authors.affiliation: Department of Prosthodontics, Seoul St. Mary's Dental Hospital, The Catholic University of Korea, #505 Banpo-dong, Seocho-gu, Seoul 137-701, Republic of Korea.
authors.forename: Su-Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Young-Pil
authors.initials: YP
authors.lastname: Yun
authors.forename: Hae-Ryong
authors.initials: HR
authors.lastname: Song
authors.forename: Heung Jae
authors.initials: HJ
authors.lastname: Chun
authors.forename: Dae Hyeok
authors.initials: DH
authors.lastname: Yang
authors.forename: Kyeongsoon
authors.initials: K
authors.lastname: Park
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Kim
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry*; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000235:genetics; D011107:Polylysine / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D055396:Bone Morphogenetic Protein 2; D055767:Immobilized Proteins; D015675:Osteocalcin; D011107:Polylysine; D006493:Heparin; D014025:Titanium; D000469:Alkaline Phosphatase; D002118:Calcium
keywords: Bone morphogenic protein-2 (BMP-2); Heparin; Osteoblast activity; Titanium