title: Mapping of active replication origins in vivo in thaum- and euryarchaeal replicons.
abstract: We report mapping of active replication origins in thaum- and euryarchaeal replicons using high-throughput sequencing-based marker frequency analysis. The chromosome of the thaumarchaeon Nitrosopumilus maritimus is shown to contain a single origin of replication, whereas the main chromosome in the halophilic euryarchaea Haloferax mediterranei and Haloferax volcanii each contains two origins. All replication origins specified bidirectional replication, and the two origins in the halophiles were initiated in synchrony. The pHM500 plasmid of H. mediterranei is shown to contain a single origin, and the copy numbers of five plasmid replicons in the two halophiles were inferred to be close to that of the main chromosome. Origin recognition boxes (ORBs) that provide binding sites for Orc1/Cdc6 replication initiator proteins are identified at all chromosomal origins, as well as in a range of additional thaumarchaeal species. An annotation update is provided for all three species.
authors.affiliation: Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Svante Arrhenius väg 20C, SE-106 91, Stockholm, Sweden.
authors.forename: Erik A
authors.initials: EA
authors.lastname: Pelve
authors.forename: Willm
authors.initials: W
authors.lastname: Martens-Habbena
authors.forename: David A
authors.initials: DA
authors.lastname: Stahl
authors.forename: Rolf
authors.initials: R
authors.lastname: Bernander
mesh_terms: D001105:Archaea / Q000145:classification* / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D002874:Chromosome Mapping; D019847:Chromosomes, Archaeal; D018628:Gene Dosage; D019766:Genes, Archaeal; D020745:Genome, Archaeal; D019621:Haloferax / Q000145:classification / Q000235:genetics; D008969:Molecular Sequence Data; D051738:Origin Recognition Complex / Q000378:metabolism*; D010957:Plasmids; D018741:Replication Origin
chemical_list: D051738:Origin Recognition Complex