title: Accurate measurement of nicotinamide adenine dinucleotide (NAD⁺) with high-performance liquid chromatography.
abstract: Nicotinamide adenine dinucleotide (NAD(+)) plays a critical role in regulating numerous biological and physiological pathways including metabolism, inflammation, cancer, and aging in mammals. Here we describe a highly quantitative method with reverse-phase high-performance liquid chromatography (HPLC) for the determination of NAD(+) levels in cells and tissues. This methodology provides accurate, reliable, and reproducible results of NAD(+) measurement, which enables us to analyze various pathophysiological changes in NAD(+) levels in vitro and in vivo.
authors.affiliation: Center for Human Nutrition, Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Yoshino
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Imai
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D006801:Humans; D051379:Mice; D009243:NAD / Q000032:analysis* / Q000378:metabolism; D041681:NIH 3T3 Cells; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009243:NAD