title: [HPLC and UPLC-MS detection of 5-HMF from rabbit ncurolymph after treated with Cornus officinalis].
abstract: OBJECTIVE
  To detect 5-HMF from rabbit ncurolymph after given different dosage of Cornus officinalis via intragastric administration by HPLC and UPLC-MS.
  
  
  METHODS
  Rabbit ncurolymph was cramped out three days after given low, medium, and high dosage of Cornus officinalis. 5-HMF from rabbit ncurolymph was detected with HPLC and UPLC-MS, respectively.
  
  
  RESULTS
  5-HMF from rabbit ncurolymph was detected with HPLC method only in rabbits given high dose group. Meanwhile, 5-HMF could be detected with UPLC-MS method in rabbits given medium as well as low dose group.
  
  
  CONCLUSIONS
  These results suggest that 5-HMF can cross the blood brain barrier (BBB) of rabbits and enter the rabbit ncurolymph.
authors.affiliation: Nanjing University of TCM, Nanjing 210046, China. kentnj@yahoo.cn
authors.forename: Hai
authors.initials: H
authors.lastname: Gu
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Liu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhan
authors.forename: Ming-Yan
authors.initials: MY
authors.lastname: Wang
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D029768:Cornus / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000378:metabolism*; D005638:Fruit / Q000737:chemistry; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000134:cerebrospinal fluid; D011817:Rabbits; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde