title: Behavior of human immunoglobulin G adsorption onto immobilized Cu(II) affinity hollow-fiber membranes.
abstract: Iminodiacetic acid (IDA) and tris(2-aminoethyl)amine (TREN) chelating ligands were immobilized on poly(ethylene vinyl alcohol) (PEVA) hollow-fiber membranes after activation with epichlorohydrin or butanediol diglycidyl ether (bisoxirane). The affinity membranes complexed with Cu(II) were evaluated for adsorption of human immunoglobulin G (IgG). The effects of matrix activation and buffer system on adsorption of IgG were studied. Isotherms of batch IgG adsorption onto finely cut membranes showed that neither of the chelates, IDA-Cu(II) or TREN-Cu(II), had a Langmuirean behavior with negative cooperativity for IgG binding. A comparison of equilibrium and dynamic maximum capacities showed that the dynamic capacity for a mini-cartridge in a cross-flow filtration mode (52.5 and 298.4 mg g(-1) dry weight for PEVA-TREN-Cu(II) and PEVA-IDA-Cu(II), respectively) was somewhat higher than the equilibrium capacity (9.2 and 73.3 mg g(-1) dry weight for PEVA-TREN-Cu(II) and PEVA-IDA-Cu(II), respectively). When mini-cartridges were used, the dynamic adsorption capacity of IDA-Cu(II) was the same for both mini-cartridge and agarose gel.
authors.affiliation: School of Chemical Engineering, University of Campinas, UNICAMP, 13083-970, Campinas, São Paulo, Brazil.
authors.forename: Mariana
authors.initials: M
authors.lastname: Borsoi-Ribeiro
authors.forename: Igor Tadeu Lazzarotto
authors.initials: IT
authors.lastname: Bresolin
authors.forename: Mookambeswaran
authors.initials: M
authors.lastname: Vijayalakshmi
authors.forename: Sônia Maria Alves
authors.initials: SM
authors.lastname: Bueno
mesh_terms: D000327:Adsorption; D002413:Cations, Divalent; D002614:Chelating Agents / Q000737:chemistry; D002846:Chromatography, Affinity; D003300:Copper / Q000737:chemistry*; D004811:Epichlorohydrin / Q000737:chemistry; D004852:Epoxy Compounds / Q000737:chemistry; D005029:Ethylenediamines / Q000737:chemistry; D007098:Imino Acids / Q000737:chemistry; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007700:Kinetics; D008567:Membranes, Artificial; D011145:Polyvinyls / Q000737:chemistry; D011485:Protein Binding; D012996:Solutions; D013816:Thermodynamics
chemical_list: D002413:Cations, Divalent; D002614:Chelating Agents; D004852:Epoxy Compounds; D005029:Ethylenediamines; D007098:Imino Acids; D007074:Immunoglobulin G; D008567:Membranes, Artificial; D011145:Polyvinyls; D012996:Solutions; C016269:bisoxirane; D004811:Epichlorohydrin; C020320:ethylene-vinyl alcohol copolymer; C099539:tris(2-aminoethyl)amine; D003300:Copper; C008109:iminodiacetic acid
keywords: Cu(II); IMAC; adsorption; affinity membranes; human IgG; tris(2-aminoethyl)amine