title: Transcriptional activation of melanocortin 2 receptor accessory protein by PPARγ in adipocytes.
abstract: Adrenocorticotropic hormone (ACTH) in rodents decreases lipid accumulation and body weight. Melanocortin receptor 2 (MC2R) and MC2R accessory protein (MRAP) are specific receptors for ACTH in adipocytes. Peroxisome proliferator-activated receptor γ (PPARγ) plays a role in the transcriptional regulation of metabolic pathways such as adipogenesis and β-oxidation of fatty acids. In this study we investigated the transcriptional regulation of MRAP expression during differentiation of 3T3-L1 cells. Stimulation with ACTH affected lipolysis in murine mature adipocytes via MRAP. Putative peroxisome proliferator response element (PPRE) was identified in the MRAP promoter region. In chromatin immunoprecipitation and reporter assays, we observed binding of PPARγ to the MRAP promoter. The mutagenesis experiments showed that the -1209/-1198 region of the MRAP promoter could function as a PPRE site. These results suggest that PPARγ is required for transcriptional activation of the MRAP gene during adipogenesis, which contributes to understanding of the molecular mechanism of lipolysis in adipocytes.
authors.affiliation: Department of Biology, Research Institute for Basic Science, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Nam Soo
authors.initials: NS
authors.lastname: Kim
authors.forename: Yoon-Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Si Young
authors.initials: SY
authors.lastname: Cho
authors.forename: Tae Ryong
authors.initials: TR
authors.lastname: Lee
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Kim
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002454:Cell Differentiation; D055785:Gene Knockdown Techniques; D008066:Lipolysis; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D047495:PPAR gamma / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D015533:Transcriptional Activation
chemical_list: C560868:MRAP protein, mouse; D008565:Membrane Proteins; D047495:PPAR gamma; D000324:Adrenocorticotropic Hormone
keywords: ACTH; HSL; MC2R; MRAP; PPARγ