title: Characterisation of CMP-sialic acid transporter substrate recognition.
abstract: CMP-sialic acid transporter: We report an in-depth, multidisciplinary, structural study that has identified the amino acid residues intimately involved in CMP-sialic acid transporter (CST) substrate specificity. Our data provide a significant contribution towards a better understanding the structure-function relationship of this important family of transporters and the rational design of CST inhibitors.
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus Queensland, 4222 (Australia).
authors.forename: Andrea
authors.initials: A
authors.lastname: Maggioni
authors.forename: Mark
authors.initials: M
authors.lastname: von Itzstein
authors.forename: Ingrid Bibiana
authors.initials: IB
authors.lastname: Rodríguez Guzmán
authors.forename: Angel
authors.initials: A
authors.lastname: Ashikov
authors.forename: Alexandre S
authors.initials: AS
authors.lastname: Stephens
authors.forename: Thomas
authors.initials: T
authors.lastname: Haselhorst
authors.forename: Joe
authors.initials: J
authors.lastname: Tiralongo
mesh_terms: D000595:Amino Acid Sequence; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003568:Cytidine Monophosphate / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D027361:Organic Anion Transporters / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013379:Substrate Specificity; D027981:Symporters / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D027361:Organic Anion Transporters; D027981:Symporters; C421224:sialic acid transport proteins; D003568:Cytidine Monophosphate
keywords: CMP-sialic acid transporter; NMR spectroscopy; nucleotide sugar transporters; sialic acids; site-directed mutagenesis