title: [Screening of plasma tumor biomarkers for hypopharyngeal carcinoma by proteomics method].
abstract: OBJECTIVE
  To screen tumor biomarkers in the plasma close related with hypopharyngeal carcinoma.
  
  
  METHODS
  Pooled plasma from 6 patients with hypopharyngeal carcinoma and 6 healthy individuals were collected. After removal of high-abundance plasma proteins, two-dimensional gel electrophoresis (2-DE) was performed to isolate the total proteins, and the protein spots with more than 2-fold differential expressions were detected by 2D analysis software followed by identification by MALDI-TOF/TOF mass spectrometer. Western blotting was performed to validate the expression level of α2-HS-glycoprotein.
  
  
  RESULTS
  A total of 11 differentially expressed protein spots were selected, including 5 upregulated proteins and 6 downregulated proteins. MALDI-TOF/TOF identified the upregulated proteins in hypopharyngeal carcinoma patients as alpha-2-HS-glycoprotein and haptoglobin and downregulated ones as Ig kappa chain C region and apolipoprotein A-I. Western blotting demonstrated that α-2-HS- glycoprotein expression level was consistent with that detected by 2-DE.
  
  
  CONCLUSIONS
  Patients with hypopharyngeal carcinoma show different plasma protein profiles from healthy individuals. These differentially expressed proteins may serve as potential specific tumor biomarkers for hypopharyngeal carcinoma.
authors.affiliation: Department of Otorhinolaryngology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. E-mail: jzli2008@yahoo.com.cn.
authors.forename: Junzheng
authors.initials: J
authors.lastname: Li
authors.forename: Wendong
authors.initials: W
authors.lastname: Tian
authors.forename: Xiong
authors.initials: X
authors.lastname: Liu
authors.forename: Shuiwang
authors.initials: S
authors.lastname: Hu
authors.forename: Bao
authors.initials: B
authors.lastname: Zhang
authors.forename: Xiangping
authors.initials: X
authors.lastname: Li
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D001798:Blood Proteins / Q000378:metabolism*; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000097:blood*; D008297:Male; D008875:Middle Aged; D040901:Proteomics / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins