title: Biodegradation of chestnut shell and lignin-modifying enzymes production by the white-rot fungi Dichomitus squalens, Phlebia radiata.
abstract: As a discarded lignocellulosic biomass, chestnut shell is of great potential economic value, thus a sustainable strategy is needed and valuable for utilization of this resource. Herein, the feasibility of biological processes of chestnut shell with Dichomitus squalens, Phlebia radiata and their co-cultivation for lignin-modifying enzymes (LMEs) production and biodegradation of this lignocellulosic biomass was investigated under submerged cultivation. The treatment with D. squalens alone at 12 days gained the highest laccase activity (9.42 ± 0.73 U mg(-1)). Combined with the data of laccase and manganese peroxidase, oxalate and H2O2 were found to participate in chestnut shell degradation, accompanied by a rapid consumption of reducing sugar. Furthermore, specific surface area of chestnut shell was increased by 77.6-114.1 % with the selected fungi, and total pore volume was improved by 90.2 % with D. squalens. Meanwhile, the surface morphology was observably modified by this fungus. Overall, D. squalens was considered as a suitable fungus for degradation of chestnut shell and laccase production. The presence of LMEs, H2O2 and oxalate provided more understanding for decomposition of chestnut shell by the white-rot fungi.
authors.affiliation: Department of Food Science and Nutrition, Key Laboratory for Food Microbial Technology of Zhejiang Province, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Ya-Chen
authors.initials: YC
authors.lastname: Dong
authors.forename: Yi-Ning
authors.initials: YN
authors.lastname: Dai
authors.forename: Teng-Yang
authors.initials: TY
authors.lastname: Xu
authors.forename: Jin
authors.initials: J
authors.lastname: Cai
authors.forename: Qi-He
authors.initials: QH
authors.lastname: Chen
mesh_terms: D031319:Aesculus / Q000382:microbiology; D005656:Fungal Proteins / Q000096:biosynthesis*; D042845:Laccase / Q000096:biosynthesis*; D008031:Lignin / Q000378:metabolism*; D009754:Nuts; D011124:Polyporaceae / Q000201:enzymology* / Q000254:growth & development
chemical_list: D005656:Fungal Proteins; D008031:Lignin; D042845:Laccase