title: n-3 PUFAs enhance the frequency of murine B-cell subsets and restore the impairment of antibody production to a T-independent antigen in obesity.
abstract: The role of n-3 polyunsaturated fatty acids (PUFA) on in vivo B-cell immunity is unknown. We first investigated how n-3 PUFAs impacted in vivo B-cell phenotypes and antibody production in the absence and presence of antigen compared with a control diet. Lean mice consuming n-3 PUFAs for 4 weeks displayed increased percentage and frequency of splenic transitional 1 B cells. Upon stimulation with trinitrophenylated-lipopolysaccharide, n-3 PUFAs increased the number of splenic transitional 1/2, follicular, premarginal, and marginal zone B cells. n-3 PUFAs also increased surface, but not circulating, IgM. We next tested the effects of n-3 PUFAs in a model of obesity that is associated with suppressed humoral immunity. An obesogenic diet after ten weeks of feeding, relative to a lean control, had no effect on the frequency of B cells but lowered circulating IgM upon antigen stimulation. Administration of n-3 PUFAs to lean and obese mice increased the percentage and/or frequency of transitional 1 and marginal zone B cells. Furthermore, n-3 PUFAs in lean and obese mice increased circulating IgM relative to controls. Altogether, the data show n-3 PUFAs enhance B cell-mediated immunity in vivo, which has implications for immunocompromised populations, such as the obese.
authors.affiliation: Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC.
authors.forename: Heather
authors.initials: H
authors.lastname: Teague
authors.forename: Cassie J
authors.initials: CJ
authors.lastname: Fhaner
authors.forename: Mitchel
authors.initials: M
authors.lastname: Harris
authors.forename: David M
authors.initials: DM
authors.lastname: Duriancik
authors.forename: Gavin E
authors.initials: GE
authors.lastname: Reid
authors.forename: Saame Raza
authors.initials: SR
authors.lastname: Shaikh
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D016175:B-Lymphocyte Subsets / Q000166:cytology* / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000276:immunology; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D007075:Immunoglobulin M / Q000096:biosynthesis* / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000097:blood / Q000276:immunology*; D010641:Phenotype; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D013997:Time Factors
chemical_list: D000941:Antigens; D015525:Fatty Acids, Omega-3; D007075:Immunoglobulin M
keywords: docosahexaenoic acid; eicosapentaenoic acid; humoral immunity