title: Camellia sinensis neuroprotective role in experimentally induced hydrocephalus in Wistar rats.
abstract: OBJECTIVE
  This study tested possible neuroprotective effects of Camellia sinensis-extracted polyphenols in experimental hydrocephalus in young rats.
  
  
  METHODS
  Seven-day-old Wistar rats were used in this study. Pups were subjected to hydrocephalus induction by 20 % kaolin intracisternal injection. The polyphenol was administered intraperitoneally for 9 or 20 days from the induction of hydrocephalus. Clinical observations and behavioral tests were performed once a day. The animals, deeply anesthetized, were sacrificed by cardiac perfusion with saline 10 or 21 days after induction of hydrocephalus and their brains were removed. Preparations were made for histological analysis by hematoxylin and eosin, solochrome-cyanine, and immunohistochemistry for GFAP.
  
  
  RESULTS
  Histopathological analysis showed that animals treated with the polyphenol for 9 consecutive days displayed reduction on astrocyte activity on the corpus callosum and external capsule, shown by GFAP immunostaining. They also displayed thicker and myelinated corpus callosum, exhibiting a more intense solochrome-cyanine blue staining.
  
  
  CONCLUSIONS
  Although these results demonstrate a possible neuroprotective effect at the initial onset of the disease, additional studies should be performed to obtain an effective and safe therapy for deeper studies in clinical trials.
authors.affiliation: Department of Surgery and Anatomy-Anatomy Division, Faculty of Medicine of Ribeirão Preto, University of São Paulo, 3900 Bandeirantes Avenue, Ribeirão Preto, São Paulo, 14049-900, Brazil.
authors.forename: Carlos Henrique Rocha
authors.initials: CH
authors.lastname: Catalão
authors.forename: Diego Augusto Leme
authors.initials: DA
authors.lastname: Correa
authors.forename: Samuel Takashi
authors.initials: ST
authors.lastname: Saito
authors.forename: Luiza da Silva
authors.initials: Lda S
authors.lastname: Lopes
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology*; D028241:Camellia sinensis; D004195:Disease Models, Animal; D006849:Hydrocephalus / Q000473:pathology*; D007150:Immunohistochemistry; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018696:Neuroprotective Agents; D010936:Plant Extracts