title: Is transplantation of cryopreserved ovarian tissue from patients with advanced-stage breast cancer safe? A pilot study.
abstract: OBJECTIVE
  To assess the safety of reimplantation of cryopreserved ovarian tissue from advanced-stage breast cancer patients.
  
  
  METHODS
  Cryopreserved ovarian cortical fragments were obtained from 13 advanced-stage breast cancer patients aged 17-35 years. After thawing, part of the ovarian cortical tissue was grafted to severe combined immunodeficient mice for 6 months. The presence of malignant mammary cells in ovarian tissue was evaluated after thawing as well as after grafting by 1) histology and immunohistochemistry (epithelial membrane antigen, Her2/neu and gross cystic disease fluid protein 15 identification), and 2) detection of the MGB2 gene by qPCR.
  
  
  RESULTS
  No malignant cells were evidenced by histology and immunohistochemistry. None of the mice died during the 6-month grafting period, nor developed macroscopically visible masses. MGB2 gene expression was detected by qPCR and confirmed by sequencing in frozen-thawed ovarian tissue in 4 cases and in grafts in 1 case.
  
  
  CONCLUSIONS
  This pilot study is the first to evaluate the risk of contamination of cryopreserved ovarian tissue from advanced-stage breast cancer patients by xenotransplantation for 6 months to immunodeficient mice, associated with more conventional screening methods. Our xenografting results are reassuring, but caution needs to be exercised, as MGB2 gene expression was detected in some cases. Larger numbers of ovarian tissue samples from patients with advanced-stage breast cancer are required to confirm our findings before ovarian tissue transplantation can be contemplated in these patients.
authors.affiliation: Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.
authors.forename: V
authors.initials: V
authors.lastname: Luyckx
authors.forename: J F
authors.initials: JF
authors.lastname: Durant
authors.forename: A
authors.initials: A
authors.lastname: Camboni
authors.forename: S
authors.initials: S
authors.lastname: Gilliaux
authors.forename: C A
authors.initials: CA
authors.lastname: Amorim
authors.forename: A
authors.initials: A
authors.lastname: Van Langendonckt
authors.forename: L M
authors.initials: LM
authors.lastname: Irenge
authors.forename: J L
authors.initials: JL
authors.lastname: Gala
authors.forename: J
authors.initials: J
authors.lastname: Donnez
authors.forename: M M
authors.initials: MM
authors.lastname: Dolmans
mesh_terms: D000293:Adolescent; D000328:Adult; D000369:Aged, 80 and over; D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology*; D002352:Carrier Proteins / Q000378:metabolism; D015925:Cryopreservation; D005260:Female; D059247:Fertility Preservation / Q000379:methods*; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D060788:Mammaglobin B / Q000096:biosynthesis / Q000235:genetics; D026901:Membrane Transport Proteins; D051379:Mice; D016513:Mice, SCID; D006080:Ovarian Follicle / Q000637:transplantation*; D010865:Pilot Projects; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D014183:Transplantation, Heterologous; D055815:Young Adult
chemical_list: D002352:Carrier Proteins; D006023:Glycoproteins; D060788:Mammaglobin B; D026901:Membrane Transport Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; C488892:PIP protein, human; C556467:SCGB2A1 protein, human; C508053:ERBB2 protein, human