title: Molecular cloning and transcriptional regulation of an allograft inflammatory factor-1 (AIF-1) in Zhikong scallop Chlamys farreri.
abstract: The allograft inflammatory factor-1 (AIF-1) is one of the key factors associated with inflammatory response. In the present study, the full-length cDNA of AIF-1 was identified from Zhikong scallop Chlamys farreri (named as CfAIF-1) by EST (expressed sequence tag) analysis and RACE (rapid-amplification of cDNA ends) approaches. The cDNA of CfAIF-1 consisted of a 5-terminal untranslated region (UTR) of 58 bp, a 3-UTR of 607 bp with a poly (A) tail, and an open reading frame (ORF) of 468 bp encoding a polypeptide of 155 amino acids with the putative molecular mass of 17.8 kDa. There was an EF hand Ca(2+)-binding motif in the deduced amino acid sequence of CfAIF-1 which was conserved in other AIF-1s. CfAIF-1 shared closer phylogenetic relationship with invertebrate counterparts than vertebrate. The mRNA transcripts of CfAIF-1 were dominantly expressed in hepatopancreas, hemocytes and adductor. During scallop ontogenesis, the CfAIF-1 mRNA was expressed at a low level at early developmental stages from eggs to blastula, and then increased significantly from gastruta to late veliger larvae (P<0.05). Moreover, the mRNA expression levels of CfAIF-1 in the hemocytes of adult scallop were significantly up-regulated during 12-48 h after LPS, PGN and poly I:C stimulation (P<0.01), but there was no significant fluctuation detected after glucan stimulation. Furthermore, the challenge of bacteria Vibrio anguillarum remarkably induced the mRNA expression of CfAIF-1 in hemocytes at 6h (P<0.05) and 12h (P<0.01). All these results collectively indicated that CfAIF-1 might be involved in the immune response during the ontogenesis and contribute to the defense against microbe infection in scallops.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.forename: Lingling
authors.initials: L
authors.lastname: Wang
authors.forename: Limei
authors.initials: L
authors.lastname: Qiu
authors.forename: Feng
authors.initials: F
authors.lastname: Yue
authors.forename: Chuanyan
authors.initials: C
authors.lastname: Yang
authors.forename: Linsheng
authors.initials: L
authors.lastname: Song
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D006434:Hemocytes / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D008018:Life Cycle Stages / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D049897:Pectinidae / Q000235:genetics* / Q000254:growth & development / Q000276:immunology / Q000378:metabolism; D010802:Phylogeny; D011485:Protein Binding; D016415:Sequence Alignment; D014158:Transcription, Genetic; D014733:Vibrio / Q000502:physiology
chemical_list: D002135:Calcium-Binding Proteins; D008070:Lipopolysaccharides
keywords: AIF-1; Allograft inflammatory factor-1; Bacteria challenge; Chlamys farreri; EST; Immune response; L; LPS; Larval development; MHC; ORF; PAMP; PAMP stimulation; PCR; PGN; RACE; RT-PCR; UTR; allograft inflammatory factor-1; base pair(s); bp; cDNA; complementary deoxyribonucleic acid; expressed sequence tag; lipopolysaccharides; liter; major histocompatibility complex; min; minute; open reading frame; pattern associated molecular pattern; peptidoglycan; polymerase chain reaction; rapid-amplification of cDNA ends; real-time PCR; untranslated region