title: 3-Bromo-1-hydroxy-9,10-anthraquinone (BHAQ) inhibits growth and migration of the human breast cancer cell lines MCF-7 and MDA-MB231.
abstract: Breast cancer is becoming more prominent in women today. As of now, there are no effective treatments in treating metastatic breast cancer. We have tested the cytotoxic and anti-migration effects of BHAQ, a synthesized anthraquinone, on two breast cancer cell lines, MCF-7 and MDA-MB231. Anthraquinones are an interesting class of molecules that display a wide spectrum of biological applications, including anticancer properties. Cellular inhibition was tested through a MTT assay, double acridine orange/propidium iodide staining and FACS cell cycle analysis. Inhibition of migration was tested by the wound healing method, and migration through a Boyden chamber. BHAQ was cytotoxic towards both cell lines in a dose dependent and possibly cell-dependent manner. Additionally, BHAQ also inhibited the migration of the highly metastatic MDA-MB231 cell line.
authors.affiliation: Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43400 Serdang, Malaysia.
authors.forename: Nadiah
authors.initials: N
authors.lastname: Abu
authors.forename: M Nadeem
authors.initials: MN
authors.lastname: Akhtar
authors.forename: Wan Yong
authors.initials: WY
authors.lastname: Ho
authors.forename: Swee Keong
authors.initials: SK
authors.lastname: Yeap
authors.forename: Noorjahan Banu
authors.initials: NB
authors.lastname: Alitheen
mesh_terms: D000880:Anthraquinones / Q000138:chemical synthesis / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D001943:Breast Neoplasms; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D020128:Inhibitory Concentration 50; D061986:MCF-7 Cells; D013629:Tamoxifen / Q000494:pharmacology
chemical_list: C587199:3-bromo-1-hydroxy-9,10-anthraquinone; D000880:Anthraquinones; D000970:Antineoplastic Agents; D013629:Tamoxifen; D004317:Doxorubicin