title: Arsenic affects on cerebellar development of mice.
abstract: To evaluate arsenic affects on the development of cerebellum, the mother mice received drinking water containing arsenic trioxide (As2O3) 4 ppm during gestation and lactation period. The cytogenesis was observed by immunohistochemical technique using 5'-bromo-2'-deoxyuridine (BrdU) antibody. To characterize the arsenic neurotoxicity, 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), an oxidative DNA lesion marker, and 8-nitroguanine, a nitrative DNA lesion marker were used to verify DNA damage. Arsenic-exposed litters showed neuron necrosis. The mitosis of granule cells decreased dramatically in arsenic-exposed mice as compared with arsenic unexposed mice. 8-OxodG was formed in neurons of all the layers, especially in the granular layer in cerebellum of arsenic-exposed mice. There is no significant difference, however, in the expression of 8-nitroguanine between arsenic-exposed and -unexposed mice. These results indicate that arsenic can disturb the mitosis of granule cells and interfere with the normal development of mice cerebellum. Arsenic-induced pathological changes in vivo may be caused by oxidative DNA damage other than nitrative DNA damage.
authors.affiliation: Histology and Embryology Department, Shenyang Medical College , Shenyang , China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Ding
authors.forename: Qiuxiang
authors.initials: Q
authors.lastname: Su
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
authors.forename: Jing
authors.initials: J
authors.lastname: Cong
authors.forename: Luan
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D000077237:Arsenic Trioxide; D001152:Arsenicals; D002531:Cerebellum / Q000187:drug effects* / Q000196:embryology / Q000254:growth & development / Q000473:pathology; D004249:DNA Damage; D005260:Female; D007150:Immunohistochemistry; D007774:Lactation; D018811:Maternal Exposure / Q000009:adverse effects*; D051379:Mice; D008813:Mice, Inbred ICR; D008938:Mitosis / Q000187:drug effects; D010087:Oxides / Q000633:toxicity*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced* / Q000473:pathology; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D001152:Arsenicals; D010087:Oxides; D014874:Water Pollutants, Chemical; D000077237:Arsenic Trioxide