title: Colony PCR.
abstract: Colony PCR is a method for rapidly screening colonies of yeast or bacteria that have grown up on selective media following a transformation step, to verify that the desired genetic construct is present, or to amplify a portion of the construct.
authors.affiliation: Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA.
authors.forename: Megan
authors.initials: M
authors.lastname: Bergkessel
authors.forename: Christine
authors.initials: C
authors.lastname: Guthrie
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D005822:Genetic Vectors; D057166:High-Throughput Screening Assays; D016133:Polymerase Chain Reaction / Q000379:methods*; D014169:Transformation, Bacterial / Q000235:genetics
keywords: Colony PCR; DNA extraction; DNA-intercalating dye; Primer synthesis; Product visualization; Thermostable polymerase