title: Quantitative analysis of cellular lipids by nano-electrospray ionization mass spectrometry.
abstract: Lipid analysis performed by nano-electrospray ionization mass spectrometry is a highly sensitive method for quantification of lipids including all lipid species of a given lipid class. Various instrumental setups are used for quantitative lipid analysis, including different modes of ionization, separation, and detection. Here we describe a work-flow for the rapid and quantitative analysis of lipid species from cellular membranes by direct infusion of lipid extracts to a nano-electrospray ionization triple quadrupole/linear ion trap mass spectrometer.
authors.affiliation: Heidelberg University Biochemistry Center (BZH), Heidelberg, Germany.
authors.forename: Cagakan
authors.initials: C
authors.lastname: Özbalci
authors.forename: Timo
authors.initials: T
authors.lastname: Sachsenheimer
authors.forename: Britta
authors.initials: B
authors.lastname: Brügger
mesh_terms: D005591:Chemical Fractionation / Q000379:methods; D008055:Lipids / Q000032:analysis / Q000737:chemistry*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D012015:Reference Standards
chemical_list: D008055:Lipids