title: Near-infrared light-directed RNAi using a photosensitive carrier molecule.
abstract: Controlled activation of small RNAs, such as small interfering RNA, in cells is very useful for various biological applications. Light is an effective inducer of controlled activation; in particular, near-infrared light is favorable because it can penetrate deeper into tissues than UV or visible light. In this study, near-infrared light control of RNA interference (RNAi) was demonstrated in mammalian cells using a photosensitive RNA carrier molecule, consisting of an RNA carrier protein and a fluorochrome. The photosensitive carrier molecule was identified from six candidates, each with a different fluorochrome. Using this carrier molecule, cytosolic RNA delivery and RNAi can be triggered by near-infrared light. Cytotoxicity was not observed after photoinduction of RNAi.
authors.affiliation: Department of Biotechnology, Graduate School of Natural Science and Technology, Okayama University , 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Matsushita-Ishiodori
authors.forename: Mika
authors.initials: M
authors.lastname: Morinaga
authors.forename: Kazunori
authors.initials: K
authors.lastname: Watanabe
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohtsuki
mesh_terms: D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D003412:Cricetulus; D004337:Drug Carriers / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000235:genetics; D007259:Infrared Rays; D008958:Models, Molecular; D017319:Photosensitizing Agents / Q000737:chemistry*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000737:chemistry / Q000235:genetics; D026082:Singlet Oxygen / Q000032:analysis
chemical_list: D004337:Drug Carriers; D005456:Fluorescent Dyes; D017319:Photosensitizing Agents; D034741:RNA, Small Interfering; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D026082:Singlet Oxygen