title: Membrane destabilization by monomeric hIAPP observed by imaging fluorescence correlation spectroscopy.
abstract: Monomeric hIAPP significantly destabilizes both model and live cell membranes by increasing membrane fluidity. This interaction with membranes happens via carpet formation followed by lipid extraction in a concentration dependent manner and thus we propose that hIAPP aggregation prior to membrane interaction may not be necessary for its cytotoxicity.
authors.affiliation: Departments of Biological Sciences and Chemistry and NUS Centre for Bio-Imaging Sciences (CBIS), National University of Singapore, 117557, Singapore. twohland@nus.edu.sg.
authors.forename: Nirmalya
authors.initials: N
authors.lastname: Bag
authors.forename: Ashraf
authors.initials: A
authors.lastname: Ali
authors.forename: Virander Singh
authors.initials: VS
authors.lastname: Chauhan
authors.forename: Thorsten
authors.initials: T
authors.lastname: Wohland
authors.forename: Aseem
authors.initials: A
authors.lastname: Mishra
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism; D006801:Humans; D058228:Islet Amyloid Polypeptide / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D008560:Membrane Fluidity; D008856:Microscopy, Fluorescence; D009010:Monte Carlo Method; D017433:Protein Structure, Secondary; D012235:Rhodamines / Q000737:chemistry
chemical_list: D058228:Islet Amyloid Polypeptide; D008051:Lipid Bilayers; D012235:Rhodamines