title: In silico optimization and low structured kinetic model of poly[(R)-3-hydroxybutyrate] synthesis by Cupriavidus necator DSM 545 by fed-batch cultivation on glycerol.
abstract: Glycerol was utilized by Cupriavidus necator DSM 545 for production of poly-3-hydroxybutyrate (PHB) in fed-batch fermentation. Maximal specific growth rates (0.12 and 0.3h(-1)) and maximal specific non-growth PHB production rate (0.16 g g(-1)h(-1)) were determined from two experiments (inocula from exponential and stationary phase). Saturation constants for nitrogen (0.107 and 0.016 g L(-1)), glycerol (0.05 g L(-1)), non-growth related PHB synthesis (0.011 g L(-1)) and nitrogen/PHB related inhibition constant (0.405 g L(-1)), were estimated. Five relations for specific growth rate were tested using mathematical models. In silico performed optimization procedures (varied glycerol/nitrogen ratio and feeding) has resulted in a PHB content of 70.9%, shorter cultivation time (23 h) and better PHB yield (0.347 g g(-1)). Initial concentration of biomass 16.8 g L(-1) and glycerol concentration in broth between 3 and 5 g L(-1) were decisive factors for increasing of productivity.
authors.affiliation: Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6/IV, HR-10000 Zagreb, Croatia. Electronic address: ivrana@pbf.hr.
authors.forename: Ivna Vrana
authors.initials: IV
authors.lastname: Spoljarić
authors.forename: Markan
authors.initials: M
authors.lastname: Lopar
authors.forename: Martin
authors.initials: M
authors.lastname: Koller
authors.forename: Alexander
authors.initials: A
authors.lastname: Muhr
authors.forename: Anna
authors.initials: A
authors.lastname: Salerno
authors.forename: Angelika
authors.initials: A
authors.lastname: Reiterer
authors.forename: Predrag
authors.initials: P
authors.lastname: Horvat
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000096:biosynthesis*; D019149:Bioreactors; D003198:Computer Simulation; D020118:Cupriavidus necator / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D007700:Kinetics; D008956:Models, Chemical
chemical_list: D005947:Glucose; D005990:Glycerol; D020155:3-Hydroxybutyric Acid
keywords: Cupriavidus necator; Glycerol; Mathematical modeling; Optimization; PHB