title: Biohybrid nanostructured iron oxide nanoparticles and Satureja hortensis to prevent fungal biofilm development.
abstract: Cutaneous wounds are often superinfected during the healing process and this leads to prolonged convalescence and discomfort. Usage of suitable wound dressings is very important for an appropriate wound care leading to a correct healing. The aim of this study was to demonstrate the influence of a nano-coated wound dressing (WD) on Candida albicans colonization rate and biofilm formation. The modified WD was achieved by submerging the dressing pieces into a nanofluid composed of functionalized magnetite nanoparticles and Satureja hortensis (SO) essential oil (EO). Chemical composition of the EO was established by GC-MS. The fabricated nanostructure was characterized by X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), Differential Thermal Analysis (DTA) and Fourier Transform-Infrared Spectroscopy (FT-IR). The analysis of the colonized surfaces using (Scanning Electron Microscopy) SEM revealed that C. albicans adherence and subsequent biofilm development are strongly inhibited on the surface of wound dressing fibers coated with the obtained nanofluid, comparing with regular uncoated materials. The results were also confirmed by the assay of the viable fungal cells embedded in the biofilm. Our data demonstrate that the obtained phytonanocoating improve the resistance of wound dressing surface to C. albicans colonization, which is often an etiological cause of local infections, impairing the appropriate wound healing.
authors.affiliation: Otorhinolaryngology, Carol Davila University of Medicine and Pharmacy, Traian Vuia no 6, Bucharest 020956, Romania. ionangheldoc@yahoo.com
authors.forename: Ion
authors.initials: I
authors.lastname: Anghel
authors.forename: Alexandru Mihai
authors.initials: AM
authors.lastname: Grumezescu
authors.forename: Alina Maria
authors.initials: AM
authors.lastname: Holban
authors.forename: Anton
authors.initials: A
authors.lastname: Ficai
authors.forename: Alina Georgiana
authors.initials: AG
authors.lastname: Anghel
authors.forename: Mariana Carmen
authors.initials: MC
authors.lastname: Chifiriuc
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects*; D005290:Ferric Compounds / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry; D031362:Satureja / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D005290:Ferric Compounds; D009822:Oils, Volatile; C000499:ferric oxide