title: Immunolocalization of vimentin, keratin 17, Ki-67, involucrin, β-catenin and E-cadherin in cutaneous squamous cell carcinoma.
abstract: Skin squamous cell carcinoma (SCC) is a subtype of very aggressive skin cancers. To investigate if epithelial-mesenchymal transition (EMT), a process for epitheloid cells losing their polarity and cohesiveness and transform into spindle-shaped cells, occurs in skin SCC. By using immunofluorescence, we defined the immunolocalization of vimentin, Keratin 17, β-catenin, E-cadherin, Ki-67 and involucrin, in SCC samples. Our results show reduced activity of involucrin and E-cadherin, and increased expression of Ki-67, β-catenin, Keratin 17 and vimentin in SCC. These data propose that EMT really occurs in poorly differentiated SCC and keratin 17 and involucrin may be another two biomarkers for EMT.
authors.affiliation: Department of Dermatology, Lishui Central Hospital, Lishui, China.
authors.forename: Yan-Ju
authors.initials: YJ
authors.lastname: Lan
authors.forename: Huan
authors.initials: H
authors.lastname: Chen
authors.forename: Jia-Qi
authors.initials: JQ
authors.lastname: Chen
authors.forename: Qiu-Hua
authors.initials: QH
authors.lastname: Lei
authors.forename: Min
authors.initials: M
authors.lastname: Zheng
authors.forename: Zhe-Ren
authors.initials: ZR
authors.lastname: Shao
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D016022:Case-Control Studies; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology; D005260:Female; D006801:Humans; D053537:Keratin-17 / Q000378:metabolism*; D019394:Ki-67 Antigen / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011498:Protein Precursors / Q000378:metabolism*; D012878:Skin Neoplasms / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D015820:Cadherins; D053537:Keratin-17; D019394:Ki-67 Antigen; D011498:Protein Precursors; D014746:Vimentin; D051176:beta Catenin; C032253:involucrin