title: Pharmacokinetic study of amoxicillin in human plasma by solid-phase microextraction followed by high-performance liquid chromatography-triple quadrupole mass spectrometry.
abstract: Sensitive and selective analytical procedures based on high-performance liquid chromatography with mass spectrometric detection were developed for the determination of amoxicillin in human plasma samples. Samples were prepared by applying in-house manufactured molecularly imprinted solid-phase microextraction probes. The detection of target compounds was performed in multiple reaction monitoring mode. The multiple reaction monitoring detection was operated in the positive electrospray ionization mode using the transitions of m/z 366 ([M + H](+) ) → 349 for amoxicillin and m/z 390 ([M + H](+) ) → 372 for gemifloxacin. The method was validated with precision within 15% relative standard deviation and accuracy within 15% relative error. The method was successfully applied to study of the pharmacokinetics of amoxicillin in human plasma after oral administration of amoxicillin.
authors.affiliation: Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7 Street, 87-100, Torun, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Szultka
authors.forename: Rafal
authors.initials: R
authors.lastname: Krzeminski
authors.forename: Justyna
authors.initials: J
authors.lastname: Walczak
authors.forename: Marek
authors.initials: M
authors.lastname: Jackowski
authors.forename: Boguslaw
authors.initials: B
authors.lastname: Buszewski
mesh_terms: D000368:Aged; D000658:Amoxicillin / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction / Q000379:methods*
chemical_list: D000658:Amoxicillin
keywords: antibiotic drugs; high performance liquid chromatography; mass spectrometry; sample preparation techniques