title: Microbially induced selective flotation of sphalerite from galena using mineral-adapted strains of Bacillus megaterium.
abstract: The selective flotation of sphalerite from a sphalerite-galena mineral mixture has been achieved using cells and extracellular secretions of Bacillus megaterium after adaptation to the chosen minerals. The extracellular secretions obtained after thermolysis of bacterial cells adapted to sphalerite yield the highest flotation recovery of sphalerite with a selectivity index value of 24.5, in comparison to the other cellular and extra-cellular bio-reagents studied. The protein profile for the unadapted and mineral-adapted cells has been found to differ distinctly, attesting to variation in the yield and nature of extra-cellular polymeric substances (EPS). The changes induced in the bacterial cell wall components after adaptation to sphalerite or galena with respect to the contents of phosphate, uronic acid and acetylated sugars of B. megaterium have been quantified. The role of the dissolved metal ions from the minerals as well as that of the constituents of extracellular secretions in modulating the surface charge of the bacterial cells as well as the minerals under study has been confirmed using various enzymatic treatments of the bacterial cells. It has been demonstrated that the induction of additional molecular weight protein fractions as well as the higher amount of extracellular proteins and phosphate secreted after adaptation to sphalerite vis-à-vis galena are contributory factors for the selective separation of sphalerite from galena.
authors.affiliation: Department of Materials Engineering, Indian Institute of Science, Bangalore 560 012, India.
authors.forename: B
authors.initials: B
authors.lastname: Vasanthakumar
authors.forename: H
authors.initials: H
authors.lastname: Ravishankar
authors.forename: S
authors.initials: S
authors.lastname: Subramanian
mesh_terms: D000222:Adaptation, Physiological; D000327:Adsorption; D001410:Bacillus megaterium / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D057005:Bioengineering; D002473:Cell Wall / Q000378:metabolism; D007218:Industrial Microbiology; D007854:Lead / Q000302:isolation & purification* / Q000378:metabolism; D008564:Membrane Potentials; D008903:Minerals / Q000378:metabolism; D013440:Sulfides / Q000302:isolation & purification* / Q000378:metabolism; D017967:Zinc Compounds / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008903:Minerals; D013440:Sulfides; D017967:Zinc Compounds; C018391:lead sulfide; D007854:Lead; C031238:zinc sulfide
keywords: Bacillus megaterium; Bioflotation; Galena; Mineral adaptation; Sphalerite