title: [Simultaneous determination of danshensu, hydroxysafflor yellow A, rosmarinic acid, lithospermic acid, salvianolic acid B in water extract of mixed salviae miltiorrhizae radix et rhizoma and carthami flos by HPLC].
abstract: OBJECTIVE
  To develop an HPLC method to determine the contents of danshensu, hydroxysafflor yellow A, rosmarinic acid, lithospermic acid, salvianolic acid B in the water extract of mixed Salviae Miltiorrhizae Radix et Rhizoma and Carthami Flos simultaneously.
  
  
  METHODS
  The separation were carried out at 30 degrees C on a ZORBAX Eclipse Plus C18 column (4.6 mm x 100 mm, 1.8 microm) with formic acid-500 mmol x L(-1) ammonium formate-water solution (0.5:10:90) as mobile phase A and acetonitrile-formic acid solution (100: 0.5) as mobile phase B in gradient mode at a flow rate of 0.5 mL x min(-1). Detection wavelengths were 280 nm for danshensu, rosmarinic acid, lithospermic acid, salvianolic acid B, and 380 nm for hydroxysafflor yellow A.
  
  
  RESULTS
  The 5 components were separated well with a good linearity (R2 > 0.999 3) in the range of the test concentration. The average recoveries of danshensu, hydroxysafflor yellow A, rosmarinic acid, lithospermic acid, and salvianolic acid B were 99.1%, 102%, 102%, 98.5% and 101%, respectively.
  
  
  CONCLUSIONS
  This method is simple, accurate, and repeatable.
authors.affiliation: Pharmaceutical Informatics Institute, Zhejiang University, Hangzhou 310058, China. love.liyao@gmail.com
authors.forename: Yao
authors.initials: Y
authors.lastname: Li
authors.forename: Zheng-Tai
authors.initials: ZT
authors.lastname: Guo
authors.forename: Xing-Chu
authors.initials: XC
authors.lastname: Gong
authors.forename: Hai-Bin
authors.initials: HB
authors.lastname: Qu
mesh_terms: D001572:Benzofurans / Q000032:analysis*; D002599:Chalcone / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002934:Cinnamates / Q000032:analysis*; D053630:Depsides / Q000032:analysis*; D007773:Lactates / Q000032:analysis*; D011809:Quinones / Q000032:analysis*; D027343:Rhizome / Q000737:chemistry*; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D000097123:Rosmarinic Acid
chemical_list: D001572:Benzofurans; D002599:Chalcone; D002934:Cinnamates; D053630:Depsides; D007773:Lactates; D011809:Quinones; D000097123:Rosmarinic Acid; C046833:lithospermic acid; C085278:hydroxysafflor yellow A; C035055:3,4-dihydroxyphenyllactic acid; C076944:salvianolic acid B