title: Transcriptomic analysis of incised leaf-shape determination in birch.
abstract: Plant researchers have focused much attention on leaf shape because of its importance in the identification. To evaluate the impact of intraspecies leaf-shape variation on the transcriptome, a series of Betula pendula 'Dalecarlica' and B. pendula saplings were generated through tissue culture. The leaf shapes and transcriptomes of B. pendula 'Dalecarlica' clones were compared with those of B. pendula clones. The leaf shape of B. pendula 'Dalecarlica' was incised and that of B. pendula was ovate. Transcriptome data revealed numerous changes in gene expression between B. pendula 'Dalecarlica' and B. pendula, including upregulation of 8767 unigenes and downregulation of 8379 unigenes in B. pendula 'Dalecarlica'. A pathway analysis revealed that the transport and signal transduction of auxin were altered in 'Dalecarlica', which may have contributed to its altered leaf shape. These results shed light on variation in birch leaf shape and help identify important genes for the genetic engineering of birch trees.
authors.affiliation: State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China; Forestry College, Beihua University, 3999 Binjiang East Road, Jilin 132013, China.
authors.forename: Huaizhi
authors.initials: H
authors.lastname: Mu
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Guifeng
authors.initials: G
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Jiang
mesh_terms: D029662:Betula / Q000033:anatomy & histology / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D053858:Metabolic Networks and Pathways / Q000235:genetics; D008954:Models, Biological; D058977:Molecular Sequence Annotation; D018515:Plant Leaves / Q000033:anatomy & histology* / Q000235:genetics*; D017423:Sequence Analysis, RNA; D059467:Transcriptome / Q000235:genetics / Q000502:physiology
keywords: 6-benzylamino purine; 6BA; Auxin; Betula pendula; COG; CTAB; DEUs; FDR; GA3; GO; Incised leaf; KEGG; Kyoto Encyclopedia of Genes and Genomes; NGS; RPKM; Transcriptome; WPM; cluster of orthologous groups of proteins; differential expression of unigenes; false discovery rate; gene ontology; gibberellin A3; hexadecyltrimethylammonium bromide; next generation sequencing; reads per kb per million reads; woody plant medium