title: [Study on rapid screening and identifying hepatotoxic compounds in toosendan fructus].
abstract: Fluorescein diacetate-labeled HepG2 cells model and flouresence automatic microscopy screening assay were used for fast screening 23 components from Toosendan Fructus, in which 5 components showed significant toxicity on HepG2 cells. The 10 compounds in the 2 components were tentatively identified with LC-MS(n), and 3 of them (meliasenin B, trichilinin D and 1-O-tigloy-1-O-debenzoylohchinal) were prepared and identified. Further experiments showed that the 3 compounds displayed dose-dependent toxicity on HepG2 cells, suggesting that these compounds in Toosendan Fructus may cause hepatotoxicity.
authors.affiliation: Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. zhaoxiaoping@zjtcm.net
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Zhao
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Ge
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Zhang
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Lan
authors.forename: Bo-Li
authors.initials: BL
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D064420:Drug-Related Side Effects and Adverse Reactions; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000633:toxicity*; D005638:Fruit / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000187:drug effects*; D031585:Melia / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal