title: Analysis of the interaction between membrane proteins and soluble binding partners by surface plasmon resonance.
abstract: The interaction between membrane proteins and their (protein) ligands is conventionally investigated by nonequilibrium methods such as co-sedimentation or pull-down assays. Surface Plasmon Resonance can be used to monitor such binding events in real-time using isolated membranes immobilized to a surface providing insights in the kinetics of binding under equilibrium conditions. This application provides a fast, automated way to detect interacting species and to determine the kinetics and affinity (Kd) of the interaction.
authors.affiliation: Department of Molecular Microbiology, University of Groningen, Groningen, The Netherlands.
authors.forename: Zht Cheng
authors.initials: ZC
authors.lastname: Wu
authors.forename: Jeanine
authors.initials: J
authors.lastname: de Keyzer
authors.forename: Ilja
authors.initials: I
authors.lastname: Kusters
authors.forename: Arnold J M
authors.initials: AJ
authors.lastname: Driessen
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D002458:Cell Fractionation / Q000379:methods; D029968:Escherichia coli Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism; D040081:Protein Array Analysis / Q000379:methods; D011485:Protein Binding; D012270:Ribosomes / Q000378:metabolism; D000069816:SEC Translocation Channels; D000081416:SecA Proteins; D013347:Subcellular Fractions; D020349:Surface Plasmon Resonance / Q000379:methods*; D022161:Transport Vesicles / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D000069816:SEC Translocation Channels; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins