title: Selenium compounds prevent amyloid β-peptide neurotoxicity in rat primary hippocampal neurons.
abstract: Neuropathological hallmarks of Alzheimer's disease (AD) include amyloid plaque formation, neurofibrillary tangles, neuronal and synaptic loss. This study aims to identify the neuroprotective effects of the selenium compounds on the neurotoxicity of amyloid β(1-42) in primary cultures of murine hippocampal neurons. Samples were subjected to immunocytochemistry and western blotting techniques to determine the role of treatments on neuronal viability and synaptic protein SNAP-25. We observed a reduced cell viability amyloid β-peptide (1-42)-induced. When cells were co-treated with amyloid β-peptide (1-42) and selenium compounds, we verified a strong increase in relative cell viability and in the level of synaptic marker synaptosomal-associated protein SNAP-25 induced by selenium compounds.
authors.affiliation: Programa de Pós-Graduação em Saúde e Comportamento, Universidade Católica de Pelotas, Rua Gonçalves Chaves 373, sala 324, Pelotas, RS, CEP 96010-280, Brazil.
authors.forename: Gabriela Lorea
authors.initials: GL
authors.lastname: Godoi
authors.forename: Lisiane
authors.initials: L
authors.lastname: de Oliveira Porciúncula
authors.forename: Janaína Fagundes
authors.initials: JF
authors.lastname: Schulz
authors.forename: Fernanda Neutzling
authors.initials: FN
authors.lastname: Kaufmann
authors.forename: João Batista
authors.initials: JB
authors.lastname: da Rocha
authors.forename: Diogo Onofre Gomes
authors.initials: DO
authors.lastname: de Souza
authors.forename: Gabriele
authors.initials: G
authors.lastname: Ghisleni
authors.forename: Hiram Larangeira
authors.initials: HL
authors.lastname: de Almeida
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D001393:Azoles / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects; D054833:Isoindoles; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016566:Organoselenium Compounds / Q000494:pharmacology*; D010446:Peptide Fragments / Q000633:toxicity*; D051381:Rats; D050825:Synaptosomal-Associated Protein 25 / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D001393:Azoles; D054833:Isoindoles; D018696:Neuroprotective Agents; D016566:Organoselenium Compounds; D010446:Peptide Fragments; C494210:Snap25 protein, rat; D050825:Synaptosomal-Associated Protein 25; C075222:amyloid beta-protein (1-42); C042986:ebselen