title: Enantioselective bioaccumulation of diniconazole in Tenebrio molitor larvae.
abstract: The enantioselective bioaccumulation of diniconazole in Tenebrio molitor Linne larva was investigated with liquid chromatography tandem mass spectrometry based on the ChiralcelOD-3R[cellulose tri-(3,5-dimethylphenyl carbamate)] column. In this study we documented the effects of dietary supplementation with wheat bran contaminated by racemic diniconazole at two dose levels of 20 mg kg(-1) and 2 mg kg(-1) (dry weight) in Tenebrio molitor. The results showed that both doses of diniconazole were taken up by Tenebrio molitor rapidly in the first few days, the concentrations of R-enantiomer and S-enantiomer at high doses reached the highest level of 0.55 mg kg(-1) and 0.48 mg kg(-1) , respectively, on the 1(st) d, and the concentrations of them obtained a maxima of 0.129 mg kg(-1) and 0.128 mg kg(-1) at low dose, respectively, on the 3(rd) d, which means that the concentration of diniconazole was proportional to the time of achieving the highest accumulated level. It afterwards attained equilibrium after a sharp decline at both 20 mg kg(-1) and 2 mg kg(-1) of diniconazole. The determination results from the feces of Tenebrio molitor demonstrated that the extraction recovery (ER) values of the high dose group were higher than that of the low dose group and the values were all above 1; therefore, it could be inferred that enantiomerization existed in Tenebrio molitor. Additionally, the biota accumulation factor was used to evaluate the bioaccumulation of diniconazole enantiomers, showing that the bioaccumulation of diniconazole in Tenebrio molitor was enantioselective with preferential accumulation of S-enantiomer.
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China; College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Liu
authors.forename: Xiao Tian
authors.initials: XT
authors.lastname: LV
authors.forename: Wen Xue
authors.initials: WX
authors.lastname: Zhu
authors.forename: Hao Yang
authors.initials: HY
authors.lastname: QU
authors.forename: Yong Xin
authors.initials: YX
authors.lastname: Gao
authors.forename: Bao Yuan
authors.initials: BY
authors.lastname: Guo
authors.forename: Hui Li
authors.initials: HL
authors.lastname: Wang
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D004043:Dietary Fiber; D005659:Fungicides, Industrial / Q000032:analysis / Q000493:pharmacokinetics / Q000633:toxicity; D007814:Larva / Q000737:chemistry / Q000187:drug effects; D013058:Mass Spectrometry; D008958:Models, Molecular; D013237:Stereoisomerism; D013712:Tenebrio / Q000737:chemistry* / Q000187:drug effects; D014230:Triazoles / Q000032:analysis / Q000493:pharmacokinetics* / Q000633:toxicity
chemical_list: D004043:Dietary Fiber; D005659:Fungicides, Industrial; D014230:Triazoles; C049346:diniconazole
keywords: Tenebrio molitor; bioaccumulation; diniconazole; enantioselectivity