title: Crystallization and preliminary X-ray crystallographic analysis of the variable domain of Scl2.3, a streptococcal collagen-like protein from invasive M3-type Streptococcus pyogenes.
abstract: Streptococcal collagen-like proteins (Scls) are widely expressed by the well recognized human pathogen Streptococcus pyogenes. These surface proteins contain a signature central collagen-like region and an amino-terminal globular domain, termed the variable domain, which is protruded away from the cell surface by the collagen-like domain. Despite their recognized importance in bacterial pathogenicity, no structural information is presently available on proteins of the Scl class. The variable domain of Scl2 from invasive M3-type S. pyogenes has successfully been crystallized using vapour-diffusion methods. The crystals diffracted to 1.5 Å resolution and belonged to space group H32, with unit-cell parameters a = 44.23, b = 44.23, c = 227.83 Å. The crystal structure was solved by single-wavelength anomalous dispersion using anomalous signal from a europium chloride derivative.|
authors.affiliation: Institute of Biostructures and Bioimaging, CNR, Via Mezzocannone 16, 80134 Naples, Italy.
authors.forename: Flavia
authors.initials: F
authors.lastname: Squeglia
authors.forename: Beth
authors.initials: B
authors.lastname: Bachert
authors.forename: Maria
authors.initials: M
authors.lastname: Romano
authors.forename: Slawomir
authors.initials: S
authors.lastname: Lukomski
authors.forename: Rita
authors.initials: R
authors.lastname: Berisio
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003094:Collagen / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D013297:Streptococcus pyogenes / Q000737:chemistry* / Q000235:genetics / Q000472:pathogenicity
chemical_list: D001426:Bacterial Proteins; D020033:Protein Isoforms; D011994:Recombinant Proteins; C423453:Scl2 protein, Streptococcus; D003094:Collagen
keywords: Scl2; Streptococcus pyogenes; collagen-like protein; group A streptococcus