title: Evaluation of serum arginase I as an oxidative stress biomarker in a healthy Japanese population using a newly established ELISA.
abstract: OBJECTIVE
  We reported previously that serum arginase I increased in asthmatic patients and was associated with oxidative stress in a small healthy population. However, the exact association of arginase I with oxidative stress is not known. The present study aimed to analyze the association of arginase I with oxidative stress in a larger healthy population by a newly established ELISA.
  
  
  METHODS
  The new ELISA for the measurement of human arginase I was established by generating recombinant arginase I protein in human arginase I gene-transfected Escherichia coli via an ARG1 cDNA fragment-inserted vector and -specific antibody in rabbits. Serum arginase I was evaluated in a cross-sectional study on a healthy population (n=721) by comparing a commercial ELISA kit with the new ELISA.
  
  
  RESULTS
  The mean levels of serum arginase I were 20.3 ± 0.7 ng/mL and 4.7 ± 0.2 ng/mL using the commercial ELISA kit and the new ELISA, respectively. Arginase I was correlated with WBC, RBC, hs-CRP, 8-OHdG, HDL-c, ALT, and BMI. Logistic regression analysis showed independent positive associations of arginase I with WBC, RBC, and urinary 8-OHdG and inverse independent associations with serum insulin and age. The association of arginase I with hs-CRP was not independent.
  
  
  CONCLUSIONS
  The independent associations of arginase I with urinary 8-OHdG and serum insulin may reflect its involvement in oxidative stress and diabetes mellitus.
authors.affiliation: Department of Public Health, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan. Electronic address: kogino@md.okayama-u.ac.jp.
authors.forename: Keiki
authors.initials: K
authors.lastname: Ogino
authors.forename: Ikuo
authors.initials: I
authors.lastname: Murakami
authors.forename: Da-Hong
authors.initials: DH
authors.lastname: Wang
authors.forename: Yorika
authors.initials: Y
authors.lastname: Tsukiyama
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Takahashi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kubo
authors.forename: Noriko
authors.initials: N
authors.lastname: Sakano
authors.forename: Heri
authors.initials: H
authors.lastname: Setiawan
authors.forename: Masahiko
authors.initials: M
authors.lastname: Bando
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Ohmoto
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000818:Animals; D001119:Arginase / Q000097:blood*; D044466:Asian People; D015415:Biomarkers / Q000097:blood / Q000652:urine; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006262:Health; D006801:Humans; D007564:Japan; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress; D011817:Rabbits; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D016622:Silver Staining; D018709:Statistics, Nonparametric
chemical_list: D015415:Biomarkers; D011933:Reagent Kits, Diagnostic; D000080242:8-Hydroxy-2'-Deoxyguanosine; C574619:ARG1 protein, human; D001119:Arginase; D003849:Deoxyguanosine
keywords: Arginase I; ELISA; Oxidative stress; Population study