title: Enzyme assisted extraction of polysaccharides from the fruit of Cornus officinalis.
abstract: Process of enzyme assisted extraction (EAE) of polysaccharides from Cornus officinalis was optimized by response surface methodology (RSM). The influence of four different factors on the yield of C. officinalis polysaccharides (COP) was studied. Results showed that the optimal conditions were compound enzyme amount of 2.15%, extraction pH of 4.2, extraction temperature of 55 °C and extraction time of 97 min. Under these conditions, the COP yield was 9.29±0.31%, which was well in agreement with the value predicted by the model. The three methods, EAE, hot water extraction (HWE), ultrasound-assisted extraction (UAE) for extracting COP by RSM were further compared. Results showed that EAE had the largest yield of polysaccharides with lower equipment cost.
authors.affiliation: Huaiyin Institute of Technology, School of Life Science and Chemical Engineering, Huaian 223001, China. youqhong@163.com
authors.forename: Qinghong
authors.initials: Q
authors.lastname: You
authors.forename: Xiulian
authors.initials: X
authors.lastname: Yin
authors.forename: Yuping
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D055598:Chemical Phenomena; D029768:Cornus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D006867:Hydrolases / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D014465:Ultrasonics; D014867:Water / Q000737:chemistry
chemical_list: D011134:Polysaccharides; D014867:Water; D006867:Hydrolases
keywords: Cornus officinalis; Enzyme assisted extraction; Response surface methodology; The yield of Cornus officinalis polysaccharides