title: SERS study of different configurations of pharmaceutical and natural product molecules ginsenoside Rg3 under the interaction with human serum albumin on simple self-assembled substrate.
abstract: Surface-enhanced Raman scattering (SERS) and fluorescence spectroscopy were employed to probe the interaction of the pharmaceutical and natural product molecules, 20(R) and 20(S)-ginsenoside Rg3, with human serum albumin (HSA). Normal Raman spectra of 20(R) and 20(S)-ginsenoside Rg3 were obtained from solid powder on glass slide. Based on the splitting peaks near 1440 cm(-1), the stacking modes of 20(R) and 20(S)-ginsenoside Rg3 were quite different. SERS spectra of both R and S configurations were obtained from a colloidal silver surface on a self-assembled SERS substrate, the most enhanced modes of 20(R) and 20(S)-ginsenoside Rg3 were those with certain motions perpendicular to the metal surface. The SERS spectra were used to predict a common orientation geometry for the alkyl chain portion of the drugs on the colloidal surface with a minor difference in the carbocyclic rings. Nevertheless, once combined with HSA, the flexible portion of alkyl chains assumes a collectively similar conformation on the Ag surface with the glucose rings perpendicularly plugging into the hydrophobic site of HSA.
authors.affiliation: Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, PR China. Electronic address: zhangwei@caas.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Bai
authors.forename: Yingping
authors.initials: Y
authors.lastname: Wang
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Hou
authors.forename: Yinping
authors.initials: Y
authors.lastname: Jin
authors.forename: Daqing
authors.initials: D
authors.lastname: Zhao
mesh_terms: D001688:Biological Products / Q000737:chemistry* / Q000378:metabolism; D036145:Ginsenosides / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008956:Models, Chemical; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000737:chemistry* / Q000378:metabolism; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism*; D013059:Spectrum Analysis, Raman; D013237:Stereoisomerism; D013499:Surface Properties
chemical_list: D001688:Biological Products; D036145:Ginsenosides; D004364:Pharmaceutical Preparations; D012709:Serum Albumin; C097367:ginsenoside Rg3
keywords: Ginsenoside Rg3; Human serum albumin; Interaction; Surface-enhanced Raman scattering