title: Hepatoprotective effects of Ixora parviflora extract against exhaustive exercise-induced oxidative stress in mice.
abstract: Ixora parviflora, a species of the Rubiaceae, is rich in polyphenols and flavonoids, and has been traditionally used as a folk medicine. An I. parviflora extract (IPE) has great antioxidant activity in vitro, including a scavenging effect on superoxide radicals, reducing power, and ferrous ion-chelating ability. However, whether IPE is efficacious against oxidative damage in vivo is not known. The purpose of this study was to determine the protective effects of IPE treatment on hepatic oxidative stress and antioxidant defenses after exhaustive exercise in mice. Fifty male C57BL/6 mice (6 week old) were randomly divided into five groups and designated a sedentary control with vehicle (C), and exhaustive exercise with vehicle (IPE0), low dosage (IPE10), medium dosage (IPE50) and high dosage (IPE100) of IPE at 0, 10, 50, and 100 mg/kg, respectively. After a single bout of exhaustive swimming exercise challenge, levels of blood ammonia and creatine kinase (CK), and hepatic superoxide dismutase (SOD) protein expression, thiobarbituric acid-reactive substance (TBARS), and gp91(phox), p22(phox), and p47(phox) subunits of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase expressions in the IPE0 group were significantly affected compared to those of the C group, but they were all significantly inhibited by the IPE treatments. Results of the present in vivo study in mice indicate that I. parviflora extract possesses antioxidative and hepatoprotective potential following exhaustive exercise.
authors.affiliation: Graduate Institute of Athletics and Coaching Science, National Taiwan Sport University, Taoyuan 33301, Taiwan.
authors.forename: Nai-Wen
authors.initials: NW
authors.lastname: Kan
authors.forename: Wen-Ching
authors.initials: WC
authors.lastname: Huang
authors.forename: Wan-Teng
authors.initials: WT
authors.lastname: Lin
authors.forename: Chih-Yang
authors.initials: CY
authors.lastname: Huang
authors.forename: Kuo-Ching
authors.initials: KC
authors.lastname: Wen
authors.forename: Hsiu-Mei
authors.initials: HM
authors.lastname: Chiang
authors.forename: Chi-Chang
authors.initials: CC
authors.lastname: Huang
authors.forename: Mei-Chich
authors.initials: MC
authors.lastname: Hsu
mesh_terms: D000284:Administration, Oral; D000641:Ammonia / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D003402:Creatine Kinase / Q000097:blood; D019610:Cytoprotection; D019587:Dietary Supplements; D004353:Drug Evaluation, Preclinical; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019255:NADPH Oxidases / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D005082:Physical Exertion; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D019666:Rubiaceae / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D013550:Swimming; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D000641:Ammonia; D013482:Superoxide Dismutase; D019255:NADPH Oxidases; D003402:Creatine Kinase