title: Mycobacterium paragordonae sp. nov., a slowly growing, scotochromogenic species closely related to Mycobacterium gordonae.
abstract: A previously undescribed, slowly growing, scotochromogenic mycobacterial strain (49061(T)) was isolated from a patient with pulmonary infections during the hsp65-sequence-based identification of Korean clinical isolates. Its 16S rRNA gene sequence was unique and the phylogenetic analysis based on 16S rRNA gene sequence (1393 bp) placed the organism into the slow-growing Mycobacterium group close to Mycobacterium gordonae (99.0 % sequence similarity). Growth characteristics and acid-fastness also supported the placement of this species into the genus Mycobacterium. Phenotypically, this strain was generally similar to Mycobacterium gordonae; however, of particular interest, the optimal growth temperature of strain 49061(T) was 25-30 °C, and it was not able to grow at 37 °C on 7H10 agar slants. Unique MALDI-TOF MS profiles of lipids, phylogenetic analysis based on another two gene sequences (hsp65 and rpoB) and a low DNA-DNA relatedness (46.52±0.7) strongly supported the taxonomic status of this strain as a representative of a distinct species from M. gordonae. It was concluded that the strain represents a novel species for which the name Mycobacterium paragordonae is proposed with the type strain 49061(T) ( = JCM 18565(T) = KCTC 29126(T)).
authors.affiliation: Department of Microbiology and Immunology, and Liver Research Institute, College of Medicine, Seoul National University, Seoul, Republic of Korea.
authors.forename: Byoung-Jun
authors.initials: BJ
authors.lastname: Kim
authors.affiliation: Department of Microbiology and Immunology, and Liver Research Institute, College of Medicine, Seoul National University, Seoul, Republic of Korea.
authors.forename: Seok-Hyun
authors.initials: SH
authors.lastname: Hong
authors.affiliation: Department of Microbiology and Immunology, and Liver Research Institute, College of Medicine, Seoul National University, Seoul, Republic of Korea.
authors.forename: Yoon-Hoh
authors.initials: YH
authors.lastname: Kook
authors.affiliation: Department of Microbiology and Immunology, and Liver Research Institute, College of Medicine, Seoul National University, Seoul, Republic of Korea.
authors.forename: Bum-Joon
authors.initials: BJ
authors.lastname: Kim
mesh_terms: D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D008171:Lung Diseases / Q000382:microbiology; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000382:microbiology; D009170:Nontuberculous Mycobacteria; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013183:Sputum / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S