title: Transcriptional activity and expression of liver X receptor in the ascidian Halocynthia roretzi.
abstract: Liver X receptors, LXRs, are ligand-activated transcription factors that belong to the group H nuclear receptor (NR) superfamily. In this study, an LXR (HrLXR) cDNA was cloned from the ascidian Halocynthia roretzi hepatopancreas and characterized to examine the functional conservation of ancestral LXRs in chordates. A phylogenetic analysis of HrLXR showed that it belongs to the tunicate (urochordate) LXR subgroup, which is distinct from vertebrate LXRs. Quantitative real-time PCR analysis revealed that HrLXR mRNA was expressed predominantly in the gills, and highly expressed in unfertilized eggs followed by decrease at later embryonic and larval stages. Unexpectedly, HrLXR was not activated by GW3965, whereas a synthetic ligand for a farnesoid X receptor, GW4064, activated HrLXR. This activation was abolished by the deletion of 51 amino acids from the N-terminus. In a mammalian two-hybrid system, HrLXR interacted with HrRXR in the presence of GW4064 or 9-cis retinoic acid. The injection of GW3965 and GW4064 in vivo increased the ATPbinding cassette sub-family G member 4 and HrLXR mRNA levels in the hepatopancreas and gills. These results suggest that the mRNA expression and transcriptional properties of HrLXR are different from those of vertebrate LXRs, although HrLXR is likely responsive to the related NR ligand, GW4064.
authors.affiliation: 1 Department of Marine Molecular Biotechnology, Gangneung-Wonju National University, Gangneung 210-702, Republic of Korea.
authors.forename: Ahmed Ahmed
authors.initials: AA
authors.lastname: Raslan
authors.forename: Jung Hwan
authors.initials: JH
authors.lastname: Lee
authors.forename: Jihye
authors.initials: J
authors.lastname: Shin
authors.forename: Yun Kyung
authors.initials: YK
authors.lastname: Shin
authors.forename: Young Chang
authors.initials: YC
authors.lastname: Sohn
mesh_terms: D000818:Animals; D001565:Benzoates / Q000494:pharmacology; D001596:Benzylamines / Q000494:pharmacology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D057809:HEK293 Cells; D006801:Humans; D007555:Isoxazoles / Q000494:pharmacology; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014561:Urochordata / Q000187:drug effects / Q000378:metabolism*
chemical_list: D001565:Benzoates; D001596:Benzylamines; D018076:DNA, Complementary; C473027:GW 3965; D007555:Isoxazoles; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors; D012333:RNA, Messenger; C412815:GW 4064