title: An application of outer membrane protein p6-specific enzyme-linked immunosorbent assay for detection of haemophilus influenzae in middle ear fluids and nasopharyngeal secretions.
abstract: An enzyme-linked immunosorbent assay specific to outer membrane protein P6 (P6-ELISA) was applied for detecting Haemophilus influenzae in middle ear fluids (MEFs) from acute otitis media (AOM) patients and in nasopharyngeal secretions (NPSs) from acute rhinosinusitis patients. P6-ELISA had a sensitivity of 83.3% for MEFs and 71.5% for NPSs and a specificity of 85.6% for MEFs and 92.5% for NPSs, respectively. Real-time PCR exhibited significant differences in the number of ompP1 gene copies among samples determined by P6-ELISA to be positive and negative for H. influenzae. However, because the P6-ELISA test has the reactivity in Haemophilus species include two commensals H. haemolyticus and H. parainfluenzae, it is thus a weak method in order to detect only NTHi correctly. Consequently, diagnosis using the P6-ELISA should be based on an overall evaluation, including the results of other related examinations and clinical symptoms to prevent misleading conclusions in clinical setting.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Wakayama Medical University, Wakayama, Japan.
authors.forename: Muneki
authors.initials: M
authors.lastname: Hotomi
authors.forename: Akihisa
authors.initials: A
authors.lastname: Togawa
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Kono
authors.forename: Gen
authors.initials: G
authors.lastname: Sugita
authors.forename: Rinya
authors.initials: R
authors.lastname: Sugita
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Fujimaki
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Kamide
authors.forename: Akihiro
authors.initials: A
authors.lastname: Uchizono
authors.forename: Keiko
authors.initials: K
authors.lastname: Kanesada
authors.forename: Shoichi
authors.initials: S
authors.lastname: Sawada
authors.forename: Naohiro
authors.initials: N
authors.lastname: Okitsu
authors.forename: Hisayo
authors.initials: H
authors.lastname: Masuda
authors.forename: Hideaki
authors.initials: H
authors.lastname: Tanaka
authors.forename: Yumi
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Noboru
authors.initials: N
authors.lastname: Yamanaka
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D004432:Ear, Middle / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006192:Haemophilus Infections / Q000175:diagnosis* / Q000382:microbiology; D018073:Haemophilus Vaccines / Q000235:genetics / Q000378:metabolism*; D006193:Haemophilus influenzae / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D009305:Nasopharynx / Q000382:microbiology; D010033:Otitis Media / Q000175:diagnosis* / Q000382:microbiology; D012220:Rhinitis / Q000175:diagnosis* / Q000382:microbiology; D012680:Sensitivity and Specificity; D012852:Sinusitis / Q000175:diagnosis* / Q000382:microbiology; D055815:Young Adult
chemical_list: D001425:Bacterial Outer Membrane Proteins; D018073:Haemophilus Vaccines; C069990:P6 outer membrane protein, Haemophilus