title: Promoting effects on the proliferation and metastasis of ACC tumor cell with XAGE-1b overexpression.
abstract: Adenoid cystic carcinoma is salivary gland malignancy characterized by indolent yet relentless growth that exhibits inherent resistance to surgery, chemotherapy and radiotherapy and it also expresses genes associated with well-defined carcinogenic and metastasis processes. There is no clear role established for XAGE-1b, which is a member of the cancer testis antigen family in tumorigenesis and the metastasis process of ACC. We studied and elucidated the correlation between the proliferation and metastasis of ACC and XAGE-1b. The eukaryotic vector was constructed for XAGE-1b overexpression in ACC-2 cells, which were used for studying the proliferation and migration phenotype in vivo and in vitro. RNAi technology was used to suppress the expression of XAGE-1b in the ACC-M cell line, and shRNA expression vector was also constructed and screened for interfering XAGE-1b expression applied to ACC-M cell lines. The effects on cell migration activity with XAGE-1b overexpression were determined by QCMTM 24-Well Cell Invasion Assay in vitro, and a lung metastatic model in mice. We found decreased effects on the proliferation phenotype of ACC-M cell in vivo and in vitro with XAGE-1b downregulation, and XAGE-1b overexpression promoted the proliferation of ACC-2 cells in vivo and in vitro, while its overexpression promoted the transmembrane invasion of ACC-2 cells in vitro and metastasis in vivo of the nude mice. The proliferation in vitro of ACC-M cells and subcutaneous tumor growth of nude mice was inhibited by XAGE-1b interference. The ACC-2 cell line with XAGE-1b overexpression displayed more rapid proliferation and higher transmembrane and metastatic ability in vivo and in vitro, with more angiogenesis in the tumor tissues. XAGE-1b gene was able to influence angiogenesis directly or indirectly, leading to tumorigenesis and metastasis of ACC.
authors.affiliation: Department of Gerontology, Zhongda Hospital Affiliated to Southeast University, Nanjing 210009, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.forename: Tingxiu
authors.initials: T
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Naishuo
authors.initials: N
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics*; D003528:Carcinoma, Adenoid Cystic / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000556:secondary; D051379:Mice; D009362:Neoplasm Metastasis / Q000235:genetics* / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000473:pathology; D034741:RNA, Small Interfering / Q000235:genetics; D012468:Salivary Gland Neoplasms / Q000235:genetics* / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: D000951:Antigens, Neoplasm; D034741:RNA, Small Interfering; C413656:XAGE1A protein, human