title: The influence of angiotensin-(1-7) Mas receptor agonist (AVE 0991) on mitochondrial proteome in kidneys of apoE knockout mice.
abstract: Excessive action of angiotensin II on mitochondria has been shown to play an important role in mitochondrial dysfunction, a common feature of atherogenesis and kidney injury. Angiotensin-(1-7)/Mas receptor axis constitutes a countermeasure to the detrimental effects of angiotensin II on AT1 receptors. The aim of the study was to assess the effects of angiotensin-(1-7) peptidomimetic AVE0991 on the kidney mitochondrial proteome in widely used animal model of atherosclerosis (apoE(-/-) mice). Proteins changed in apoE(-/-) mice belonged to the groups of antioxidant enzymes, apoptosis regulators, inflammatory factors and metabolic enzymes. Importantly, AVE0991 partially reversed atherosclerosis-related changes in apoE(-/-) mice.
authors.affiliation: Chair of Pharmacology, Jagiellonian University Medical College, 16 Grzegorzecka Str, 31 531, Krakow, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Suski
authors.forename: Rafał
authors.initials: R
authors.lastname: Olszanecki
authors.forename: Aneta
authors.initials: A
authors.lastname: Stachowicz
authors.forename: Józef
authors.initials: J
authors.lastname: Madej
authors.forename: Beata
authors.initials: B
authors.lastname: Bujak-Giżycka
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Okoń
authors.forename: Ryszard
authors.initials: R
authors.lastname: Korbut
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001057:Apolipoproteins E / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D050197:Atherosclerosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D007093:Imidazoles / Q000494:pharmacology*; D018836:Inflammation Mediators / Q000378:metabolism; D007668:Kidney / Q000293:injuries / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000819:agonists* / Q000235:genetics / Q000378:metabolism
chemical_list: C469726:AVE 0991; D000975:Antioxidants; D001057:Apolipoproteins E; D051017:Apoptosis Regulatory Proteins; D007093:Imidazoles; D018836:Inflammation Mediators; D024101:Mitochondrial Proteins; D020543:Proteome; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D044140:Receptor, Angiotensin, Type 1; D043562:Receptors, G-Protein-Coupled
keywords: 2DE–LC–MS/MS; 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate; ACE2; ADF; AT1r; AT2r; Ang II; Ang IV; Ang-(1–12); Ang-(1–7); Angiotensin (1–7); Apolipoprotein E; CA2; CHAPS; CKD; COX-IV; DTT; EGTA; ERK; ESRD; GPM; GRHPR; GST P1; HPLC; HRP; Hint1; IAA; IEF; IL-1; IPG; JNK; Kidney; MAP; MIF; Mitochondrion; MnSOD; NADPH; PAS; PDI; PH; PMSF; Proteomics; Prx 5; RAS; SDS; TFA; TPP; TTBS; The Global Proteome Machine; Tween-Tris-buffered saline; actin depolymerizing factor; angiotensin II; angiotensin IV; angiotensin converting enzyme 2; angiotensin-(1–12); angiotensin-(1–7); apoE(−/−); apolipoprotein E deficient mice; c-Jun N-terminal kinase; carbonic anhydrase 2; chronic kidney disease; cytochrome c oxidase subunit IV; ditiotreitol; end stage renal disease; ethylene glycol tetraacetic acid; extracellular signal-regulated kinases; glutathione S-transferase P1; glyoxylate reductase; high-pressure liquid chromatography; histidine triad nucleotide-binding protein 1; horseradish peroxidase; immobilized pH gradient strips; interleukin 1; iodoacetamide; isoelectrofocusing; macrophage inhibitory factor; manganese superoxide dismutase; mitogen-activated protein kinase; periodic acid-Schiff stain; peroxiredoxin-5; phenylmethylsulfonyl fluoride; primary hyperoxaluria; protein disulfide isomerase; reduced nicotinamide adenine dinucleotide phosphate; renin–angiotensin system; sodium dodecyl sulfate; trans-proteomic pipeline; trifluoroacetic acid; two-dimensional electrophoresis coupled with liquid chromatography and tandem mass spectrometry; type 1 angiotensin II receptor; type 2 angiotensin II receptor