title: Occurrence and genetic characterization of Toxoplasma gondii in naturally infected pigs.
abstract: The protozoan Toxoplasma gondii is an obligate intracellular parasite that infects a wide range of warm-blooded vertebrates. The data about the occurrence of toxoplasmosis in slaughter pigs in the Slovak Republic are still missing. The aim of our study was to estimate the prevalence of toxoplasmosis in pigs from Slovakia during the period of 2006-2010 by ELISA and PCR methods. In sera of 970 slaughter pigs, 2.16% seropositivity to T. gondii was detected. In tissue samples of seropositive pigs the presence of T. gondii DNA was confirmed. In six monitored Slovak regions the seropositivity varied between 1.11 and 3.48%. The statistically significant differences were recorded between the Košice and Prešov region. The seroprevalence of toxoplasmosis in sows (4.26%) was two times higher than that in slaughter pigs (2.06%) (OR = 2.12; 95% CI = 0.48-9.36). Presence of Toxoplasma gondii in tissues of seropositive pig isolates was confirmed by TGR1E and B1 genes and analysis of DNA polymorphism at SAG2 and ROP1 genes revealed the presence of virulent strain of genotype I in 85.7% of infected pigs and an avirulent strain (genotype II) in 14.3% of pigs.
authors.affiliation: Institute of Parasitology SAS, Hlinkova 3, 040 01 Košice, Slovak Republic. ludmilaturcekova@gmail.com
authors.forename: Ľudmila
authors.initials: Ľ
authors.lastname: Turčeková
authors.forename: Daniela
authors.initials: D
authors.lastname: Antolová
authors.forename: Katarína
authors.initials: K
authors.lastname: Reiterová
authors.forename: František
authors.initials: F
authors.lastname: Spišák
mesh_terms: D000818:Animals; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005838:Genotype; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins / Q000235:genetics; D016036:Seroepidemiologic Studies; D018154:Slovakia / Q000453:epidemiology; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000469:parasitology*; D014122:Toxoplasma / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D014124:Toxoplasmosis, Animal / Q000453:epidemiology* / Q000469:parasitology*
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins