title: Self-assembly of 50 bp poly(dA)·poly(dT) DNA on highly oriented pyrolytic graphite via atomic force microscopy observation and molecular dynamics simulation.
abstract: This study has investigated the formation patterns resulting from the self-assembly of deoxyribonucleic acid (DNA) on highly oriented pyrolytic graphite (HOPG), using both experimental and molecular dynamics approaches. Under optimized conditions based on pretreatment of HOPG surface and specific solution concentrations, DNA is found to self-assemble to form various patterned networks. The associated self-assembly mechanism is elucidated using coarse-grained molecular dynamics simulations and fractal dimension analysis. The results of this work demonstrate an effective technique allowing the formation of arrays of negatively charged biomacromolecules on negatively charged HOPG surfaces.
authors.affiliation: Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Doi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takeuchi
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Nii
authors.forename: Shingo
authors.initials: S
authors.lastname: Akamatsu
authors.forename: Toshiya
authors.initials: T
authors.lastname: Kakizaki
authors.forename: Satoyuki
authors.initials: S
authors.lastname: Kawano
mesh_terms: D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D056004:Molecular Dynamics Simulation; D011067:Poly dA-dT / Q000737:chemistry*
chemical_list: D011067:Poly dA-dT; D006108:Graphite; D004247:DNA