title: Matrix-free dynamic nuclear polarization enables solid-state NMR 13C-13C correlation spectroscopy of proteins at natural isotopic abundance.
abstract: We introduce a general approach for dynamic nuclear polarization (DNP) enhanced solid-state NMR that overcomes the current problems in DNP experiments caused by the use of frozen solutions. Notably, we report for the first time a 2D (13)C-(13)C correlation spectrum of a protein without the use of isotopic labeling.
authors.affiliation: Laboratoire de Chimie Inorganique et Biologique, UMR-E3 (CEA/UJF) and CNRS, Institut Nanosciences et Cryogénie, CEA, 38054 Grenoble, France. gael.depaepe@cea.fr.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Sabine
authors.initials: S
authors.lastname: Hediger
authors.forename: Gaël
authors.initials: G
authors.lastname: De Paëpe
mesh_terms: D002247:Carbon Isotopes; D007553:Isotope Labeling; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D011506:Proteins / Q000032:analysis*
chemical_list: D002247:Carbon Isotopes; D011506:Proteins