title: Glycol chitosan-based nanogel as a potential targetable carrier for siRNA.
abstract: A self-assembled glycol chitosan nanogel (GC) is synthesized by chemically grafting hydrophobic chains onto a polysaccharide, which is comprehensively characterized. The obtained macromolecular micelle is decorated with folate-conjugated poly(ethylene glycol) (PEG) (GCFA). An average size distribution of 250 and 200 nm is observed, respectively for the GC and GCFA nanogels. Differential cell localization is observed on incubating the materials with HeLa cells. Whereas the GC nanogel is detected on the cell surface, GCFA is localized in the cytoplasm. The cell viability is not compromised by the nanogels. Interestingly, GC nanogel is poorly internalized by bone marrow derived macrophages (BMDMs), and GCFA is not phagocytosed. Given its ability to complex siRNA, the targetable GC nanogel can be a promising vehicle for siRNA delivery.
authors.affiliation: Institute for Biotechnology and Bioengineering (IBB), Centre for Biological Engineering, Campus de Gualtar, University of Minho, Braga, Portugal.
authors.forename: Paula
authors.initials: P
authors.lastname: Pereira
authors.forename: Daniela
authors.initials: D
authors.lastname: Morgado
authors.forename: Agnès
authors.initials: A
authors.lastname: Crepet
authors.forename: Laurent
authors.initials: L
authors.lastname: David
authors.forename: Francisco M
authors.initials: FM
authors.lastname: Gama
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000737:chemistry; D004337:Drug Carriers / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D058990:Molecular Targeted Therapy; D000080385:Nanogels; D011092:Polyethylene Glycols / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000737:chemistry; D011094:Polyethyleneimine / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics
chemical_list: D004337:Drug Carriers; D000080385:Nanogels; D011108:Polymers; D034741:RNA, Small Interfering; C118638:glycol-chitosan; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D048271:Chitosan
keywords: cellular uptake and siRNA delivery; cytotoxicity; glycol chitosan nanogel