title: Elevated plasma citrulline: look for dihydrolipoamide dehydrogenase deficiency.
abstract: The E3 subunit of the pyruvate dehydrogenase complex (dihydrolipoamide dehydrogenase/dihydrolipoyl dehydrogenase/DLD/lipoamide dehydrogenase/LAD), is a mitochondrial matrix enzyme and also a part of the branched-chain ketoacid dehydrogenase and alpha-ketoglutarate dehydrogenase complexes. DLD deficiency (MIM #246900), is relatively frequent in the Ashkenazi Jewish population but occurs in other populations as well. Early diagnosis is important to prevent episodes of metabolic decompensation, liver failure, and encephalopathy. The clinical presentations are varied and may include Reye-like syndrome, hepatic failure, myopathy, and myoglobinuria. Laboratory markers, such as elevated urinary alpha-ketoglutarate, blood pyruvate, lactate, and ammonia, are mostly nonspecific and not always present, making the diagnosis difficult. Since we observed elevated plasma citrulline levels in a number of confirmed cases, we retrospectively examined the value of citrulline as a biochemical marker for DLD deficiency. Data was gathered from the files of 17 pediatric patients with DLD deficiency, confirmed by enzymatic and genetic analysis. The control group included 19 patients in whom urea cycle defects were ruled out but DLD deficiency was suspected. Seven of the DLD-deficient patients presented with elevated plasma citrulline levels (median value 205 μM, range 59-282 μM) (normal range 1-45 μM) while none in the control patient group. In five patients, elevated citrulline was associated with elevated plasma glutamine and metabolic acidosis. Interestingly, elevated plasma citrulline was associated with the common G229C mutation. In conclusion, we suggest that elevated plasma citrulline in the absence of urea cycle defects warrants an investigation for DLD deficiency.
authors.affiliation: Monique and Jacques Roboh Department of Genetic Research, Hadassah, Hebrew University Medical Center, Jerusalem, Israel.
authors.forename: Ruby
authors.initials: R
authors.lastname: Haviv
authors.forename: Avraham
authors.initials: A
authors.lastname: Zeharia
authors.forename: Corinne
authors.initials: C
authors.lastname: Belaiche
authors.forename: Yishai
authors.initials: Y
authors.lastname: Haimi Cohen
authors.forename: Ann
authors.initials: A
authors.lastname: Saada
mesh_terms: D000138:Acidosis / Q000175:diagnosis / Q000235:genetics; D000140:Acidosis, Lactic / Q000175:diagnosis* / Q000235:genetics; D000483:Alleles; D057565:Apathy; D015415:Biomarkers / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D002956:Citrulline / Q000097:blood*; D004252:DNA Mutational Analysis; D008058:Dihydrolipoamide Dehydrogenase / Q000235:genetics; D059788:Dried Blood Spot Testing; D042241:Early Diagnosis; D005260:Female; D005973:Glutamine / Q000097:blood; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007557:Israel; D053609:Lethargy / Q000209:etiology / Q000235:genetics; D008297:Male; D008375:Maple Syrup Urine Disease / Q000175:diagnosis* / Q000235:genetics; D015997:Neonatal Screening; D011237:Predictive Value of Tests; D012016:Reference Values; D012189:Retrospective Studies
chemical_list: D015415:Biomarkers; D005973:Glutamine; D002956:Citrulline; D008058:Dihydrolipoamide Dehydrogenase