title: Characterisation of polysaccharides from green tea of Huangshan Maofeng with antioxidant and hepatoprotective effects.
abstract: This study was to examine the hepatoprotective effects of polysaccharides from green tea of Huangshan Maofeng (HMTP) against CCl4-induced oxidative damage in mice. HMTP is an acidic heteropolysaccharide with galactose (35.0%, mol.%), arabinose (28.9%) and galacturonic acid (11.3%) being the main monosaccharide components. HMTP (400 and 800 mg/kg·bw) administered orally daily for 14 days before CCl4 administration significantly reduced the impact of CCl4 toxicity on the serum markers of liver damage, alanine aminotransferase, aspartate aminotransferase, total-cholesterol and triglycerides. This method of HMTP administration also markedly restrained hepatic lipid peroxidation formation of malondialdehyde and 15-F2t isoprostanes, and elevated the antioxidant levels of hepatic glutathione and superoxide dismutase. These results together with liver histopathology indicated that HMTP exhibited hepatoprotection against CCl4-induced injury, which was found to be comparable to that of biphenyldicarboxylate. The hepatoprotective effects of HMTP may be due to both the inhibition of lipid peroxidation and the increase of antioxidant activity.
authors.affiliation: Key Laboratory of Ministry of Education for Medicinal Resource and Natural Pharmaceutical Chemistry, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, China.
authors.forename: Xinshan
authors.initials: X
authors.lastname: Lu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Sun
authors.forename: Su
authors.initials: S
authors.lastname: Yang
authors.forename: Xingbin
authors.initials: X
authors.lastname: Yang
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry; D001219:Aspartate Aminotransferases / Q000378:metabolism; D028241:Camellia sinensis / Q000737:chemistry*; D002251:Carbon Tetrachloride / Q000009:adverse effects; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy / Q000201:enzymology / Q000517:prevention & control*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D011134:Polysaccharides / Q000008:administration & dosage* / Q000737:chemistry; D020011:Protective Agents / Q000008:administration & dosage* / Q000737:chemistry; D013662:Tea
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D011134:Polysaccharides; D020011:Protective Agents; D013662:Tea; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: Antioxidant effect; Green tea; HPLC; Hepatoprotective effect; Polysaccharides