title: Cytotoxicity testing of temporary luting cements with two- and three-dimensional cultures of bovine dental pulp-derived cells.
abstract: This study evaluated the cytotoxicity of eugenol-containing and eugenol-free temporary luting cements. For cytotoxicity testing, bovine pulp-derived cells transfected with Simian virus 40 Large T antigen were exposed to extracts of eugenol-containing (Rely X Temp E) and eugenol-free (Provicol, PreVISION CEM, and Rely X Temp NE) temporary luting cements for 24 h. The cytotoxicity of the same materials was also evaluated in a dentin barrier test device using three-dimensional cell cultures of bovine pulp-derived cells. The results of the cytotoxicity studies with two-dimensional cultures of bovine dental pulp-derived cells revealed that cell survival with the extracts of Rely X Temp E, Provicol, PreVISION CEM, and Rely X Temp NE was 89.1%, 84.9%, 92.3%, and 66.8%, respectively. Rely X Temp NE and Provicol showed cytotoxic effects on bovine dental pulp-derived cells (P < 0.05). The results of the dentin barrier test revealed that cell survival with the above-mentioned temporary cement was 101.5%, 91.9%, 93.5%, and 90.6%, respectively. None of the temporary luting cements significantly reduced cell survival compared with the negative control in the dentin barrier test (P > 0.05). Biologically active materials released from temporary luting cements may not influence the dentine-pulp complex if the residual dentine layer is at least 0.5 mm thick.
authors.affiliation: Department of Restorative Dentistry, Faculty of Dentistry, Selcuk University, 42075 Konya, Turkey.
authors.forename: Hayriye Esra
authors.initials: HE
authors.lastname: Ülker
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ülker
authors.forename: Hasan Önder
authors.initials: HÖ
authors.lastname: Gümüş
authors.forename: Muhammet
authors.initials: M
authors.lastname: Yalçın
authors.forename: Abdulkadir
authors.initials: A
authors.lastname: Şengün
mesh_terms: D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D003738:Dental Cements / Q000633:toxicity*; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects*; D014162:Transfection
chemical_list: D003738:Dental Cements