title: Endocannabinoid crosstalk between placenta and maternal fat in a baboon model (Papio spp.) of obesity.
abstract: BACKGROUND
  Maternal obesity (MO) remains a serious obstetric problem with acute and chronic morbidities for both mothers and offspring. The mechanisms underlying these adverse consequences of MO remain unknown. Endocannabinoids (ECB) are neuromodulatory lipids released from adipocytes and other tissues. Metabolic crosstalk between placenta and adipocytes may mediate sequelae of MO. The goal of this study was to elucidate placental and systemic ECB in MO.
  
  
  METHODS
  Placentas, sera, and subcutaneous fat were collected at Cesarean sections performed near term (0.9 G) in four non-obese (nOB) and four obese (OB) baboons (Papio spp.). Concentrations of anandamide (AEA) and 2-arachidonoylglycerol (2-AG) were measured by liquid chromatography coupled to tandem mass spectrometry. AEA and 2-AG pathways were characterized in placentas by Q-RT-PCR, Western blot and immunohistochemistry.
  
  
  RESULTS
  Placental 2-AG levels were lower and maternal fat AEA levels were higher in OB (1254.1 ± 401.3 nmol/kg and 17.3 ± 4 nmol/kg) vs. nOB (3124.2 ± 557.3 nmol/kg and 3.1 ± 0.6 nmol/kg) animals. Concentrations of 2-AG correlated positively between maternal fat and placenta (r = 0.82, p = 0.013), but correlated negatively with maternal leptin concentrations (r = -0.72, p = 0.04 and r = -0.83, p = 0.01, respectively).
  
  
  CONCLUSIONS
  This is the first study to demonstrate differential ECB pathway regulation in maternal fat and placenta in MO. Differential regulation and function exist for AEA and 2-AG as the major ECB pathways in placenta.
authors.affiliation: Department of Obstetrics and Gynecology, University of Tennessee Health Science Center, Memphis, TN, USA.
authors.forename: B
authors.initials: B
authors.lastname: Brocato
authors.forename: A A
authors.initials: AA
authors.lastname: Zoerner
authors.forename: Z
authors.initials: Z
authors.lastname: Janjetovic
authors.forename: C
authors.initials: C
authors.lastname: Skobowiat
authors.forename: S
authors.initials: S
authors.lastname: Gupta
authors.forename: B M
authors.initials: BM
authors.lastname: Moore
authors.forename: A
authors.initials: A
authors.lastname: Slominski
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Schenone
authors.forename: R
authors.initials: R
authors.lastname: Phinehas
authors.forename: R J
authors.initials: RJ
authors.lastname: Ferry
authors.forename: E
authors.initials: E
authors.lastname: Dick
authors.forename: G B
authors.initials: GB
authors.lastname: Hubbard
authors.forename: G
authors.initials: G
authors.lastname: Mari
authors.forename: N
authors.initials: N
authors.lastname: Schlabritz-Loutsevitch
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000097:blood / Q000378:metabolism; D001692:Biological Transport; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D063388:Endocannabinoids / Q000097:blood / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005989:Glycerides / Q000097:blood / Q000378:metabolism; D020738:Leptin / Q000097:blood; D009765:Obesity / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D010215:Papio; D010920:Placenta / Q000378:metabolism* / Q000473:pathology; D053284:Polyunsaturated Alkamides / Q000097:blood / Q000378:metabolism; D011247:Pregnancy; D011248:Pregnancy Complications / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D043884:Receptor, Cannabinoid, CB1 / Q000235:genetics / Q000378:metabolism; D043885:Receptor, Cannabinoid, CB2 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D043882:Receptors, Cannabinoid / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D050507:Subcutaneous Fat, Abdominal / Q000378:metabolism* / Q000473:pathology; D053719:Tandem Mass Spectrometry
chemical_list: D001095:Arachidonic Acids; D063388:Endocannabinoids; D005989:Glycerides; D020738:Leptin; D053284:Polyunsaturated Alkamides; D043884:Receptor, Cannabinoid, CB1; D043885:Receptor, Cannabinoid, CB2; D043882:Receptors, Cannabinoid; C094503:glyceryl 2-arachidonate; C078814:anandamide
keywords: Endocannabinoids; Fat; Maternal obesity; Placenta