title: The down-regulation of GNAO1 and its promoting role in hepatocellular carcinoma.
abstract: GNAO1 (guanine nucleotide-binding protein, α-activating activity polypeptide O) is a member of the subunit family of Gα proteins, which are molecular switchers controlling signal transductions and whose deregulation can promote oncogenesis. HCC (hepatocellular carcinoma) is one of the malignant tumours around the world, which summons novel biomarkers or targets for effective diagnosis and treatments. The present study was aimed to investigate the expression of GNAO1 in HCC patient tissues and the possible mechanisms by which it took effects. The expression of GNAO1 was detected by IHC (immunohistochemistry) and real-time qPCR (quantitative PCR). Cell proliferation test and cell senescence test were then performed to explore the role of GNAO1 in the occurrence and development of HCC. It was revealed that the level of GNAO1 was comparably less in HCC tissues than in the adjacent tissues. Furthermore, down-regulation of GNAO1 increased cell proliferation, while suppressing the senescence of HCC cells. In conclusion, our findings revealed and confirmed the importance of GNAO1 in HCC, indicating that GNAO1 is a potential biomarker as well as a promising therapeutic target for HCC.
authors.affiliation: *Department of Digestive Diseases, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Pei
authors.affiliation: *Department of Digestive Diseases, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: *Department of Digestive Diseases, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Wu
authors.affiliation: *Department of Digestive Diseases, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Lü
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: *Department of Digestive Diseases, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Dongqin
authors.initials: D
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016922:Cellular Senescence; D015536:Down-Regulation; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: C584143:GNAO1 protein, human; D034741:RNA, Small Interfering; D019206:GTP-Binding Protein alpha Subunits, Gi-Go