title: Development of three vitrification-based cryopreservation procedures for shoot tips of China's potato.
abstract: China is now the largest potato producing country worldwide. The establishment of efficient conservation techniques for potato germplasm is a prerequisite for breeding of elite cultivars. Potato viral diseases have been a great threat for sustainable potato production in China. Use of virus-free seed tubers is an effective and practical means to control viral diseases. In the present study, three vitrification-based cryopreservation techniques, i.e. droplet-vitrification, encapsulation-vitrification and vitrification were successfully developed for the first time for China's potato. Cultivar 'Zihuabai' was used to optimize key parameters involved in the three vitrification-based procedures. With the optimized parameters, shoot regrowth percentages of 71%, 76% and 43% were obtained for droplet-vitrification, encapsulation-vitrification and vitrification, respectively. The three protocols developed were further tested with eight China's major cultivars, with average shoot regrowth of 61%, 38% and 28% for droplet-vitrification, encapsulation-vitrification and vitrification, respectively. Successful development of the three cryopreservation procedures using a single cultivar will facilitate a number of comparative studies such as cryo-injury, regrowth pattern, genetic stability and efficiency of virus elimination. Testing these three cryogenic procedures for potato major cultivars representing a wide range of genetic background, will help the establishment of potato cryobanking in China and the production of virus-free plants.
authors.affiliation: College of Horticulture, Northwest A and F University, Yangling, Shaanxi, China.
authors.forename: B
authors.initials: B
authors.lastname: Wang
authors.forename: R R
authors.initials: RR
authors.lastname: Wang
authors.forename: J W
authors.initials: JW
authors.lastname: Li
authors.forename: Y L
authors.initials: YL
authors.lastname: Ma
authors.forename: W M
authors.initials: WM
authors.lastname: Sheng
authors.forename: M F
authors.initials: MF
authors.lastname: Li
authors.forename: Q C
authors.initials: QC
authors.lastname: Wang
mesh_terms: D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000737:chemistry / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D011198:Solanum tuberosum / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D058989:Vitrification
chemical_list: D003451:Cryoprotective Agents