title: Type II Fp of human mitochondrial respiratory complex II and its role in adaptation to hypoxia and nutrition-deprived conditions.
abstract: The flavoprotein (Fp) subunit of human mitochondrial succinate-ubiquinone reductase (SQR, complex II) has isoforms (type I, type II). Type II Fp is predominantly expressed in some cancer and fetal tissues and those tissues are often exposed to ischemia. The present study shows that complex II with type II Fp has lower optimal pH than complex II with type I Fp, and type II Fp mRNA expression was induced by ischemia. The result suggests complex II with type II Fp may function in cells with low mitochondrial matrix pH caused by ischemia and its function is related to cellular adaptation to ischemia.
authors.affiliation: Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Chika
authors.initials: C
authors.lastname: Sakai
authors.forename: Eriko
authors.initials: E
authors.lastname: Tomitsuka
authors.forename: Makoto
authors.initials: M
authors.lastname: Miyagishi
authors.forename: Shigeharu
authors.initials: S
authors.lastname: Harada
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Kita
mesh_terms: D000222:Adaptation, Physiological; D001483:Base Sequence; D017931:DNA Primers; D042963:Electron Transport Complex II / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D000860:Hypoxia / Q000503:physiopathology*; D044342:Malnutrition / Q000503:physiopathology*; D008928:Mitochondria / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; C478189:respiratory complex II; D042963:Electron Transport Complex II; C511268:SDHA protein, human
keywords: 0.1mM 5,5′-dithiobis-(2-nitrobenzoic acid); 2,6-dichlorophenolindophenol; 3-NP; 3-nitropropionic acid; 50% inhibitory concentration; Complex II; DCIP; DTNB; Flavoprotein; Fp; Fumarate respiration; HIF-1α; Human mitochondria; IC(50); QFR; SDHAF1; SDHAF2; SML; SQR; Succinate-ubiquinone reductase; flavoprotein; hypoxia-inducible factor-1α; quinol-fumarate reductase; succinate-dehydrogenase assembly factor 1; succinate-dehydrogenase assembly factor 2; succinate-ubiquinone reductase; sucrose monolaurate