title: Microbial community and population dynamics of single-stage autotrophic nitrogen removal for dilute wastewater at the benchmark oxygen rate supply.
abstract: Microbial communities and their kinetic performance in a single-stage autotrophic nitrogen-removal filter at an optimal oxygen supply were examined to determine the presence and activity of denitrifiers, anaerobic ammonia-oxidizing (anammox), ammonia-oxidizing, and nitrite-oxidizing bacteria. To this end, different molecular biology techniques such as real-time quantitative polymerase chain reaction (qPCR) and biomarkers such as 16S rRNA revealed a diverse microbial community along the filter. It was important to survey the specific species of anammox bacteria using a newly designed Candidatus Brocadiafulgida (BF) specific primer, as well as Candidatus Brocadia anammoxidans (BA) and Candidatus Kuenenia stuttgartiensis (KS) specific primers. An unexpected finding was that the predominant anammox species switched from KS in concentrated wastewater to BA in dilute wastewaters. The Eckenfelder model of the NH3-N transformation along the filter was Se=S0 exp(-0.192D/L(2.3217)). These results provide a foundational understanding of the microbial structure and reaction kinetics in such systems.
authors.affiliation: Dept. of Bioenviron. Eng., Research Center for Analysis and Identification, Chung Yuan Christian University, Jhong-Li City 32023, Taiwan. Electronic address: yt_huang@cycu.edu.tw.
authors.forename: Yu-Tzu
authors.initials: YT
authors.lastname: Huang
authors.affiliation: Dept. of Bioenviron. Eng., Research Center for Analysis and Identification, Chung Yuan Christian University, Jhong-Li City 32023, Taiwan.
authors.forename: Shiou-Shiou
authors.initials: SS
authors.lastname: Chen
authors.affiliation: Dept. of Civil & Environ. Eng. and Research Institute for Sustainable Urban Development, The Hong Kong Polytechnic University, Kowloon, Hong Kong.
authors.forename: Po-Heng
authors.initials: PH
authors.lastname: Lee
authors.affiliation: Dept. of Environ. Eng., Inha University, Namgu, Yonghhyun dong 253, Incheon, Republic of Korea.
authors.forename: Jaeho
authors.initials: J
authors.lastname: Bae
mesh_terms: D007700:Kinetics; D059013:Microbial Consortia; D009584:Nitrogen / Q000302:isolation & purification*; D010100:Oxygen / Q000737:chemistry*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D062065:Wastewater / Q000737:chemistry*; D014871:Water Microbiology
chemical_list: D009584:Nitrogen; D010100:Oxygen; D012336:RNA, Ribosomal, 16S; D062065:Wastewater
keywords: Anammox; Dilute wastewater; Kinetic model; Primer; Quantitative PCR