title: Application of ZnO/graphene and S6 aptamers for sensitive photoelectrochemical detection of SK-BR-3 breast cancer cells based on a disposable indium tin oxide device.
abstract: ZnO/graphene (ZnO/G) composite and S6 aptamer were employed to sensitive photoelectrochemical (PEC) strategy for the specific detection of SK-BR-3 cancer cells based on a portable indium tin oxide microdevice. ZnO/G composite was synthesized using a facile ultrasonic method, and then applied to improve the PEC performance due to the unique hollow structure of ZnO naospheres and the superior properties of graphene. Subsequently, S6 aptamer was applied to this specific detection of SK-BR-3 cancer cells. And the concentration of SK-BR-3 cells was measured with a low detection limit of 58 cells mL(-1) and a wide linear range of 1×10(2)-1×10(6) cells mL(-1), through the decrease in photocurrent intensity resulting from the increase in steric hindrances when specifically recognized with S6 aptamers. Excellent discrimination against target and analogous cells was demonstrated, indicating the high selectivity of the proposed cell sensor. Our work also demonstrated a sensitive, stable and low cytotoxicity approach for early and accurate detection of cancer cells.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.forename: Shaowei
authors.initials: S
authors.lastname: Wang
authors.forename: Shenguang
authors.initials: S
authors.lastname: Ge
authors.forename: Xianrang
authors.initials: X
authors.lastname: Song
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D001940:Breast / Q000473:pathology*; D001943:Breast Neoplasms / Q000175:diagnosis*; D045744:Cell Line, Tumor; D055664:Electrochemical Techniques / Q000295:instrumentation; D004867:Equipment Design; D005260:Female; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D017971:Tin Compounds / Q000737:chemistry*; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D017971:Tin Compounds; C109984:indium tin oxide; D006108:Graphite; D015034:Zinc Oxide
keywords: Indium tin oxide device; Photoelectrochemical biosensor; S6 aptamers; SK-BR-3 cancer cells; ZnO/graphene