title: Supercritical CO2 extraction, chemical characterisation and antioxidant potential of Brassica oleracea var capitata against HO·, O2(·-) and ROO·.
abstract: In this work were extracted bioactive compounds from Brassica oleracea var capitata using supercritical CO2 and evaluated the antioxidant potential of the extracts. Five extractions were accomplished to investigate the influence of pressure (10-25 MPa) and temperature (20-60 °C) in the extraction yield, chemical composition and antioxidant potential towards peroxyl, superoxide and hydroxyl radicals. The highest extraction yield was obtained at 60 °C and 25 MPa, which was 0.47 wt% (run 2). In the characterisation of the extracts obtained was possible the identification of sulforaphane and iberin nitrile that present known biological properties. The extracts of all runs presented antioxidant activities towards the three radicals, but the highest activities for all radicals were using the extracts obtained in the run 2. The use of supercritical CO2 extraction to obtain bioactive compounds of B. oleracea var capitata showed to be a promising alternative to conventional extraction methods, since allowed the extraction of compounds with scientific and industrial interest.
authors.affiliation: Post-Graduate Program in Pharmaceutical Sciences, Federal University of Santa Maria, Camobi Campus, Santa Maria, RS 97105-900, Brazil.
authors.forename: Valéria
authors.initials: V
authors.lastname: Dal Prá
authors.forename: Carolina Bolssoni
authors.initials: CB
authors.lastname: Dolwitsch
authors.forename: Géssica Domingos
authors.initials: GD
authors.lastname: da Silveira
authors.forename: Liliane
authors.initials: L
authors.lastname: Porte
authors.forename: Clarissa
authors.initials: C
authors.lastname: Frizzo
authors.forename: Marcus Vinicius
authors.initials: MV
authors.lastname: Tres
authors.forename: Vinicius
authors.initials: V
authors.lastname: Mossi
authors.forename: Marcio Antonio
authors.initials: MA
authors.lastname: Mazutti
authors.forename: Paulo Cícero
authors.initials: PC
authors.lastname: do Nascimento
authors.forename: Denise
authors.initials: D
authors.lastname: Bohrer
authors.forename: Leandro Machado
authors.initials: LM
authors.lastname: de Carvalho
authors.forename: Carine
authors.initials: C
authors.lastname: Viana
authors.forename: Marcelo Barcellos
authors.initials: MB
authors.lastname: da Rosa
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification*; D001937:Brassica / Q000737:chemistry*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification*; D017382:Reactive Oxygen Species / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species
keywords: Antioxidant activity; Brassica oleracea var capitata; Chemical characterisation; Supercritical CO(2) extraction