title: Different effects of Alzheimer's peptide Aβ(1-40) oligomers and fibrils on supported lipid membranes.
abstract: Beta-amyloid (1-40) is one of the two most abundant species of amyloid-beta peptides present as fibrils in the extracellular senile plaques in the brain of Alzheimer's patients. Recently, the molecular aggregates constituting the early stage of fibril formation, i.e., oligomers and protofibrils, have been investigated as the main responsible for amyloid-beta cytotoxic effect. The molecular mechanism leading to neurodegeneration is still under debate, and it is common opinion that it may reside in the interaction between amyloid species and the neural membrane. In this investigation Atomic Force Microscopy and spectroscopy have been used to understand how structural (and mechanical) properties of POPC/POPS lipid bilayers, simulating the phospholipid composition and negative net charge of neuritic cell membranes, are influenced by the interaction with Aβ(1-40), in different stages of the peptide aggregation. Substantial differences in the damage caused to the lipid bilayers have been observed, confirming the toxic effect exerted especially by Aβ(1-40) prefibrillar oligomers.
authors.affiliation: Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Canale
authors.forename: Silvia
authors.initials: S
authors.lastname: Seghezza
authors.forename: Silvia
authors.initials: S
authors.lastname: Vilasi
authors.forename: Rita
authors.initials: R
authors.lastname: Carrotta
authors.forename: Donatella
authors.initials: D
authors.lastname: Bulone
authors.forename: Alberto
authors.initials: A
authors.lastname: Diaspro
authors.forename: Pier Luigi
authors.initials: PL
authors.lastname: San Biagio
authors.forename: Silvia
authors.initials: S
authors.lastname: Dante
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D018625:Microscopy, Atomic Force; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D010713:Phosphatidylcholines / Q000737:chemistry; D010718:Phosphatidylserines / Q000737:chemistry
chemical_list: D016229:Amyloid beta-Peptides; D008051:Lipid Bilayers; D010446:Peptide Fragments; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; C078592:amyloid beta-protein (1-40); C050294:1-palmitoyl-2-oleoylglycero-3-phosphoserine; C028694:1-palmitoyl-2-oleoylphosphatidylcholine
keywords: Aβ toxicity; Force spectroscopy; In liquid AFM; Supported lipid bilayers