title: Direct and indirect method of plant regeneration from root explants of Caesalpinia bonduc (L.) Roxb.--a threatened medicinal plant of Western Ghats.
abstract: An in vitro regeneration protocol has been standardized via direct and indirect methods from excised root explants of C. bonduc, a threatened woody legume used for the treatment of contagious diseases, inflammation, leprosy, antiperiodic, febrifuge, anthelmenthic, urinary disorders, leucorrhoea, piles and to heal wounds. MS medium supplemented with 17.75 micromol BAP and 2.46 micromol IBA, induced a mean of 3.40 +/- 1.07 shoots directly from the surface of excised root explant. Subsequently, the shoots rooted readily on MS half strength medium with out growth regulators. In indirect organogenesis, callogenic frequency was optimized (96.66%) at the concentration of 9.04 micromol 2, 4-D and 0.88 micromol BAP. An average, 15.30 +/- 5.25 shoots were differentiated from the root callus at the concentration of 17.57 micromol BAP and 2.85 micromol IAA. Shoots regenerated through callus were rooted well on MS half strength medium with growth regulators at 2.95 micromol IBA. Rooted plantlets were transferred to the pots containing sterilized soil and were successfully hardened at greenhouse condition for three weeks then exposed to the natural environment. Survival rate was more (95%) in plantlets derived through direct organogenesis than (60%) the plantlets regenerated through root calli.
authors.affiliation: P.G. Department of Studies and Research in Biotechnology and Bioinformatics, Kuvempu University, Shankaraghatta 577 451, India.
authors.forename: S R Santosh
authors.initials: SR
authors.lastname: Kumar
authors.forename: V
authors.initials: V
authors.lastname: Krishna
authors.lastname: Venkatesh
authors.forename: K
authors.initials: K
authors.lastname: Pradeepa
authors.forename: K Girish
authors.initials: KG
authors.lastname: Kumar
authors.forename: A U
authors.initials: AU
authors.lastname: Gnanesh
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D000225:Adenine / Q000494:pharmacology; D001593:Benzyl Compounds; D029911:Caesalpinia / Q000187:drug effects / Q000254:growth & development*; D003470:Culture Media; D056727:Endangered Species; D007194:India; D007210:Indoleacetic Acids / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D007701:Kinetin / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology; D058439:Plant Somatic Embryogenesis Techniques; D010946:Plants, Medicinal / Q000187:drug effects / Q000254:growth & development; D011205:Povidone / Q000494:pharmacology; D011687:Purines; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development
chemical_list: D001593:Benzyl Compounds; D003470:Culture Media; D007210:Indoleacetic Acids; D007211:Indoles; D010937:Plant Growth Regulators; D011687:Purines; C014612:indolebutyric acid; D015084:2,4-Dichlorophenoxyacetic Acid; C030737:indoleacetic acid; D011205:Povidone; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin