title: Plasticicumulans lactativorans sp. nov., a polyhydroxybutyrate-accumulating gammaproteobacterium from a sequencing-batch bioreactor fed with lactate.
abstract: A bacterial consortium that accumulated more than 90 % (w/w) polyhydroxybutyrate (PHB) from lactate was selected in a laboratory-scale bioreactor with a 'feast-famine' regime. Bacterial strain YD(T), representing a dominant species in this enrichment, was isolated and characterized. Analysis of the 16S rRNA gene sequence revealed that the isolate is a member of the class Gammaproteobacteria, forming an independent phylogenetic lineage. The closest relative of the isolate was Plasticicumulans acidivorans TUD-YJ37(T), with 94 % 16S rRNA gene sequence similarity. Strain YD(T) was an obligate aerobe with large, ovoid, Gram-negative cells, motile by means of a polar flagellum. It utilized a relatively broad spectrum of substrates (e.g. carbohydrates, fatty acids) as carbon and energy sources. The temperature range for growth was 20-45 °C, with an optimum at 40 °C; the pH range was pH 6.0-8.0, with an optimum at pH 7.0. The major respiratory lipoquinones were Q-8 (91 %) and Q-7 (9 %). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and an unidentified aminolipid. The predominant fatty acids in the membrane polar lipids were C16 : 1ω7c, C16 : 0 and C18 : 1ω7c. The G+C content of the genomic DNA was 68.5 mol%. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, the isolate is proposed to represent a novel species in the genus Plasticicumulans, for which the name Plasticicumulans lactativorans sp. nov. is proposed. The type strain is YD(T) ( = DSM 25287(T) = NCCB 100398(T)).
authors.affiliation: Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
authors.forename: Yang
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, 117811 Moscow, Russia.|Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
authors.forename: Dimitry Y
authors.initials: DY
authors.lastname: Sorokin
authors.affiliation: Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
authors.forename: Helena
authors.initials: H
authors.lastname: Junicke
authors.affiliation: Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
authors.forename: Robbert
authors.initials: R
authors.lastname: Kleerebezem
authors.affiliation: Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
authors.forename: Mark C M
authors.initials: MCM
authors.lastname: van Loosdrecht
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D019149:Bioreactors / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006885:Hydroxybutyrates / Q000378:metabolism*; D019344:Lactic Acid / Q000737:chemistry*; D008969:Molecular Sequence Data; D009426:Netherlands; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D011091:Polyesters / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D000094122:Polyhydroxybutyrates
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006885:Hydroxybutyrates; D019344:Lactic Acid; D010743:Phospholipids; D011091:Polyesters; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D024482:Vitamin K 2; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C024223:vitamin MK 8; C062629:menaquinone 7