title: Mineralized self-assembled peptides on 3D laser-made scaffolds: a new route toward 'scaffold on scaffold' hard tissue engineering.
abstract: In this study, we propose a new approach to hard tissue regeneration based on the mineralization of 3D scaffolds made using lasers. To this end, we report the rational design of aspartate-containing self-assembling peptides targeted for calcium binding. We further investigate the suitability of these peptides to support cell attachment and proliferation when coupled on a hybrid organic-inorganic structurable material, and evaluate the response of pre-osteoblastic cells on functionalized 3D scaffolds and material surfaces. Our results show that the mineralized peptide, when immobilized on a hybrid photo-structurable material strongly supports cell adhesion, a proliferation increase after three and seven days in culture, and exhibits a statistically significant increase of biomineralization. We propose this strategy as a 'scaffold on scaffold' approach for hard tissue regeneration.
authors.affiliation: Department of Materials Science and Technology, University of Crete, Vassilika Vouton, 710 03 Heraklion, Crete, Greece. Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology Hellas (FORTH), PO Box 527, Vassilika Vouton, 711 10 Heraklion, Crete, Greece.
authors.forename: Konstantina
authors.initials: K
authors.lastname: Terzaki
authors.forename: Erifyli
authors.initials: E
authors.lastname: Kalloudi
authors.forename: Estelle
authors.initials: E
authors.lastname: Mossou
authors.forename: Edward P
authors.initials: EP
authors.lastname: Mitchell
authors.forename: V Trevor
authors.initials: VT
authors.lastname: Forsyth
authors.forename: Elena
authors.initials: E
authors.lastname: Rosseeva
authors.forename: Paul
authors.initials: P
authors.lastname: Simon
authors.forename: Maria
authors.initials: M
authors.lastname: Vamvakaki
authors.forename: Maria
authors.initials: M
authors.lastname: Chatzinikolaidou
authors.forename: Anna
authors.initials: A
authors.lastname: Mitraki
authors.forename: Maria
authors.initials: M
authors.lastname: Farsari
mesh_terms: D000682:Amyloid / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001224:Aspartic Acid; D002118:Calcium / Q000378:metabolism; D002130:Calcium Phosphates / Q000378:metabolism; D002468:Cell Physiological Phenomena / Q000187:drug effects; D002478:Cells, Cultured; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D000682:Amyloid; D002130:Calcium Phosphates; D010455:Peptides; D001224:Aspartic Acid; C020243:calcium phosphate; D002118:Calcium