title: Pseudoplusia includens single nucleopolyhedrovirus: genetic diversity, phylogeny and hypervariability of the pif-2 gene.
abstract: The soybean looper (Pseudoplusia includens Walker, 1857) has become a major pest of soybean crops in Brazil. In order to determine the genetic diversity and phylogeny of variants of Pseudoplusia includens single nucleopolyhedrovirus (PsinSNPV-IA to -IG), partial sequences of the genes lef-8, lef-9, pif-2, phr and polh were obtained following degenerate PCR and phylogenetic trees constructed using maximum parsimony and Bayesian methods. The aligned sequences showed polymorphisms among the isolates, where the pif-2 gene was by far the most variable and is predicted to be under positive selection. Furthermore, some of the pif-2 DNA sequence mutations are predicted to result in significant amino acid substitutions, possibly leading to changes in oral infectivity of this baculovirus. Cladistic analysis revealed two closely related monophyletic groups, one containing PsinNPV isolates IB, IC and ID and another containing isolates IA, IE, IF and IG. The phylogeny of PsinSNPV in relation to 56 other baculoviruses was also determined from the concatenated partial LEF-8, LEF-9, PIF-2 and POLH/GRAN deduced amino acid sequences, using maximum-parsimony and Bayesian methods. This analysis clearly places PsinSNPV with the Group II Alphabaculovirus, where PsinSNPV is most closely related to Chrysodeixis chalcites NPV and Trichoplusia ni SNPV.
authors.affiliation: Departamento de Biologia Celular, Universidade de Brasília-UnB, Brasília, DF, Brazil; Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, W5 Norte Final, 70770-917 Brasília, DF, Brazil.
authors.forename: Saluana R
authors.initials: SR
authors.lastname: Craveiro
authors.forename: Fernando L
authors.initials: FL
authors.lastname: Melo
authors.forename: Zilda Maria A
authors.initials: ZM
authors.lastname: Ribeiro
authors.forename: Bergmann M
authors.initials: BM
authors.lastname: Ribeiro
authors.forename: Sônia Nair
authors.initials: SN
authors.lastname: Báo
authors.forename: Peter W
authors.initials: PW
authors.lastname: Inglis
authors.forename: Maria Elita B
authors.initials: ME
authors.lastname: Castro
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D001483:Base Sequence; D004252:DNA Mutational Analysis; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000145:classification / Q000235:genetics*; D010572:Pest Control, Biological; D010802:Phylogeny; D011110:Polymorphism, Genetic; D016415:Sequence Alignment
keywords: Genotypic variants; Phylogeny; Pseudoplusia includens SNPV; pif-2 Gene polymorphisms