title: A systematic method for analysing the protein hydration structure of T4 lysozyme.
abstract: A systematic method for the analysis of the hydration structure of proteins is demonstrated on the case study of lysozyme. The method utilises multiple structural data of the same protein deposited in the protein data bank. Clusters of high water occupancy are localised and characterised in terms of their interaction with protein. It is shown that they constitute a network of interconnected hydrogen bonds anchored to the protein molecule. The high occupancy of the clusters does not directly correlate with water-protein interaction energy as was originally hypothesised. The highly occupied clusters rather correspond to the nodes of the hydration network that have the maximum number of hydrogen bonds including both the protein atoms and the surrounding water clusters.
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nam. 2, 166 10, Prague, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Kysilka
authors.forename: J
authors.initials: J
authors.lastname: Vondrášek
mesh_terms: D017122:Bacteriophage T4 / Q000737:chemistry* / Q000201:enzymology; D030562:Databases, Protein; D006860:Hydrogen Bonding; D008958:Models, Molecular; D009113:Muramidase / Q000737:chemistry*; D013816:Thermodynamics; D014764:Viral Proteins / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D014764:Viral Proteins; D014867:Water; D009113:Muramidase
keywords: X-ray crystallography; cluster algorithm; interaction enthalpy; protein hydration structure; water