title: Transient gene expression in Medicago truncatula leaves via Agroinfiltration.
abstract: Transient expression is a powerful method for the functional characterization of genes. In this chapter, we outline a protocol for the transient expression of constructs in Medicago truncatula leaves using Agrobacterium tumefaciens infiltration. Using quantitative real-time PCR we demonstrate that the infiltration of a construct containing the LEGUME ANTHOCYANIN PRODUCTION 1 (LAP1) transcription factor results in the strong upregulation of key biosynthetic genes and the accumulation of anthocyanin pigment in the leaves after just 3 days. Thus, this method provides a rapid and powerful way to the discovery of downstream targets of M. truncatula transcription factors.
authors.affiliation: Department of Biochemistry, University of Otago, Dunedin, New Zealand.
authors.forename: Kelsey
authors.initials: K
authors.lastname: Picard
authors.forename: Robyn
authors.initials: R
authors.lastname: Lee
authors.forename: Roger
authors.initials: R
authors.lastname: Hellens
authors.forename: Richard
authors.initials: R
authors.lastname: Macknight
mesh_terms: D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D046913:Medicago truncatula / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology*; D018515:Plant Leaves / Q000235:genetics* / Q000382:microbiology*; D030821:Plants, Genetically Modified; D014170:Transformation, Genetic