title: Association Between Polymorphisms of VDR, COL1A1, and LCT genes and bone mineral density in Belarusian women with severe postmenopausal osteoporosis.
abstract: BACKGROUND AND OBJECTIVE. Variation of osteoporosis in the population is the result of an interaction between the genotype and the environment, and the genetic causes of osteoporosis are being widely investigated. The aim of this study was to analyze the association between the polymorphisms of the vitamin D receptor (VDR), type I collagen (COL1A1), and lactase (LCT) genes and severe postmenopausal osteoporosis as well as bone mineral density (BMD). MATERIAL AND METHODS. A total of 54 women with severe postmenopausal osteoporosis and 77 controls (mean age, 58.3 years [SD, 6.2] and 56.7 years [SD, 7.42], respectively) were included into the study. The subjects were recruited at the City Center for Osteoporosis Prevention (Minsk, Belarus). Dual-energy x-ray absorptiometry was used to measure bone mineral density at the lumbar spine and the femoral neck. Severe osteoporosis was diagnosed in the women with the clinical diagnosis of postmenopausal osteoporosis and at least 1 fragility fracture. The control group included women without osteoporosis. Polymorphic sites in osteoporosis predisposition genes (ApaI, BsmI, TaqI, and Cdx2 of the VDR gene, G2046T of the COL1A1 gene, and T-13910C of the LCT gene) were determined using the polymerase chain reaction on the deoxyribonucleic acid isolated from dried bloodspots. RESULTS. The data showed that the ApaI and BsmI polymorphisms of the VDR gene and T- 13910C of the LCT gene were associated with severe postmenopausal osteoporosis in the analyzed Belarusian women (P<0.01). A statistically significant positive correlation between the VDR risk genotypes ApaI and TaqI and bone mineral density was found (P<0.05). CONCLUSIONS. The findings of this study suggest that at least the ApaI and BsmI polymorphisms of the VDR gene and T-13910C of the LCT gene are associated with the risk of postmenopausal osteoporosis in our sample of the Belarusian women.
authors.affiliation: Institute of Genetics and Cytology NAS Belarus, Belarus.
authors.forename: Pavel
authors.initials: P
authors.lastname: Marozik
authors.forename: Irma
authors.initials: I
authors.lastname: Mosse
authors.forename: Vidmantas
authors.initials: V
authors.lastname: Alekna
authors.forename: Ema
authors.initials: E
authors.lastname: Rudenko
authors.forename: Marija
authors.initials: M
authors.lastname: Tamulaitienė
authors.forename: Heorhi
authors.initials: H
authors.lastname: Ramanau
authors.forename: Vaidilė
authors.initials: V
authors.lastname: Strazdienė
authors.forename: Volha
authors.initials: V
authors.lastname: Samokhovec
authors.forename: Maxim
authors.initials: M
authors.lastname: Ameliyanovich
authors.forename: Nikita
authors.initials: N
authors.lastname: Byshnev
authors.forename: Alexander
authors.initials: A
authors.lastname: Gonchar
authors.forename: Liubov
authors.initials: L
authors.lastname: Kundas
authors.forename: Krystsina
authors.initials: K
authors.lastname: Zhur
mesh_terms: D015519:Bone Density / Q000235:genetics*; D024042:Collagen Type I / Q000235:genetics*; D000090602:Collagen Type I, alpha 1 Chain; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry; D005260:Female; D006801:Humans; D043322:Lactase / Q000235:genetics*; D008875:Middle Aged; D015663:Osteoporosis, Postmenopausal / Q000453:epidemiology* / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D018167:Receptors, Calcitriol / Q000235:genetics*; D002094:Republic of Belarus / Q000453:epidemiology; D012306:Risk
chemical_list: D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D018167:Receptors, Calcitriol; D004262:DNA Restriction Enzymes; C053864:endodeoxyribonuclease BsmI; D015252:Deoxyribonucleases, Type II Site-Specific; C038485:GGGCCC-specific type II deoxyribonucleases; D043322:Lactase