title: Human mesenchymal stem cell transplantation changes proinflammatory gene expression through a nuclear factor-κB-dependent pathway in a rat focal cerebral ischemic model.
abstract: Previous studies have demonstrated the immunomodulatory functions of mesenchymal stem cells (MSCs) in cerebral ischemic rats. However, the underlying mechanisms are unclear. The purpose of this study is to investigate the effects of MSC transplantation on transcriptional regulations of proinflammatory genes in cerebral ischemia. Transient ischemia was induced by middle cerebral artery occlusion (MCAO) in adult male Sprague-Dawley rats. After 24 hr, vehicle (PBS) or a human MSC line (B10) was transplanted intravenously. The neurological deficits, infarct volume, cellular accumulations, and gene expression changes were monitored by means of behavior tests, MRI, immunohistochemistry, Western blotting, laser capture microdissection, and real-time PCR. In the core area of the B10 transplantation group, the number of ED1-positive macrophage/microglia was decreased compared with the PBS group. In the core, nuclear factor-κB (NF-κB) was decreased, although CCAAT/enhancer-binding protein β was not changed; both were expressed mainly in ED1-positive macrophage/microglia. Likewise, mRNAs of NF-κB-dependent genes including interleukin-1β, MCP-1, and inducible nitric oxide synthase were decreased in ED1-positive and Iba-1-positive macrophage/microglia in the B10 transplantation group. Moreover, upstream receptors of the NF-κB pathway, including CD40 and Toll-like receptor 2 (TLR2), were decreased. Immunofluorescence results showed that, in the B10 transplantation group, the percentages of NF-κB-positive, CD40-positive, and TLR2-positive cells were decreased in ED1-positive macrophage/microglia. Furthermore, NF-κB-positive cells in the CD40- or TLR2-expressing cell population were decreased in the B10 transplantation group. This study demonstrates that B10 transplantation inhibits NF-κB activation, possibly through inhibition of CD40 and TLR2, which might be responsible for the inhibition of proinflammatory gene expression in macrophage/microglia in the infarct lesion.
authors.affiliation: Department of Neurology, Shimane University Faculty of Medicine, Izumo, Japan.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nagai
authors.forename: Abdullah Md
authors.initials: AM
authors.lastname: Sheikh
authors.forename: Xue Yun
authors.initials: XY
authors.lastname: Liang
authors.forename: Shozo
authors.initials: S
authors.lastname: Yano
authors.forename: Shingo
authors.initials: S
authors.lastname: Mitaki
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Ishibashi
authors.forename: Shotai
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Seung U
authors.initials: SU
authors.lastname: Kim
authors.forename: Shuhei
authors.initials: S
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002545:Brain Ischemia / Q000378:metabolism*; D004195:Disease Models, Animal; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000235:genetics / Q000378:metabolism; D061346:Laser Capture Microdissection; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D016328:NF-kappa B / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D016328:NF-kappa B
keywords: Laser capture microdissection; NF-κB; TLR2; cerebral ischemia; human mesenchymal stem cell