title: Structure elucidation and antioxidant effect of a polysaccharide from Lactarius camphoratum (Bull.) Fr.
abstract: In this study, a heteropolysaccharide was isolated from the fruiting bodies of Lactarius camphoratum (Bull.) Fr. through DEAE-cellulose column and Sephadex G-200 column. The L. camphoratum (Bull.) Fr. polysaccharide (LC-1) had a molecular weight of 9279 Da and was mainly composed of β-D-Glu and α-D-Gal which ratios were 2:1. Structural features of L. camphoratum (Bull.) Fr. polysaccharide (LC-1) were investigated by a combination of total hydrolysis, methylation analysis, gas chromatography-mass spectrometry (GC-MS), infrared (IR) spectra and nuclear magnetic resonance (NMR) spectroscopy. The results indicated that L. camphoratum (Bull.) Fr. polysaccharide (LC-1) had a backbone of (1→4)-β-D-glucopyranose residues which branches at O-6 based on the experimental results. The branches were mainly composed of one with →3)-α-D-galactopyranose residue. The antioxidant activity of LC-1 was evaluated with two biochemical methods, including 1,1-diphenyl-2-picrylhydrazyl (DPPH(-)) radical scavenging, scavenging activity of 2,2'-azino-bis(3-ethylbenzthiazoline-6-suphonic acid) diammonium (ABTS(+)) radical. The results indicated that LC-1 showed strong antioxidant.
authors.affiliation: Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, 1# Shida Road, Nanchong, Sichuan Province 637009, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Yiling
authors.initials: Y
authors.lastname: Hou
authors.forename: Xiang
authors.initials: X
authors.lastname: Ding
authors.forename: Wanru
authors.initials: W
authors.lastname: Hou
authors.forename: Bo
authors.initials: B
authors.lastname: Song
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Yichun
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D001487:Basidiomycota / Q000737:chemistry*; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000494:pharmacology*; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D011134:Polysaccharides; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant assay; Lactarius camphoratum (Bull.) Fr.; Polysaccharide structure