title: Superoxide dismutase activity in portal vein endothelium after partial liver resection.
abstract: OBJECTIVE
  To investigate superoxide dismutase (SOD) activity in the portal vein endothelium and malondialdehyde acid (MDA) production in liver tissue of rats submitted to 70% hepatectomy.
  
  
  METHODS
  Twelve rats were distributed in two groups (hepatectomy and sham). Animals were sacrificed on post operative day 1 and portal vein, liver tissue and blood samples were collected. Portal vein SOD production was measured using lucigenin-amplified chemiluminescence assays. MDA measurement was used as an index of oxidative stress through the formation of TBARS (Thiobarbituric Acid Reactive Species).
  
  
  RESULTS
  There was no difference in post operative bilirubin, AST, ALT levels between groups. DHL level was higher in the hepatectomy group (p=0.01). MDA production in the remnant liver tissue and endothelial portal vein SOD activity were also significantly (p<0.05) elevated in the hepatectomy group when compared to control group. There was no correlation between MDA and SOD activity. SOD activity, on the other hand, showed a positive correlation with LDH level (p=0.038) and MDA levels showed a positive correlation with AST and ALT levels (p<0.001).
  
  
  CONCLUSIONS
  There is an increased production of malondialdehyde acid in liver tissue after partial hepatectomy and increased activity of superoxide dismutase in portal vein endothelium as well.
authors.affiliation: Medical School, University of Sao Paulo (USP), Brazil.
authors.forename: Rogério Heggendorn
authors.initials: RH
authors.lastname: Sayão Filho
authors.forename: Marcos Vinicius
authors.initials: MV
authors.lastname: Perini
authors.forename: José Arnaldo
authors.initials: JA
authors.lastname: Cruz
authors.forename: Juliana
authors.initials: J
authors.lastname: Requena
authors.forename: Hermes Vieira
authors.initials: HV
authors.lastname: Barbeiro
authors.forename: Nilza Trindade
authors.initials: NT
authors.lastname: Molan
authors.forename: Fabio Pinatel
authors.initials: FP
authors.lastname: Lopasso
authors.forename: Luiz Augusto Carneiro
authors.initials: LA
authors.lastname: D'Albuquerque
authors.forename: Ivan
authors.initials: I
authors.lastname: Cecconello
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D004730:Endothelium, Vascular / Q000201:enzymology*; D006498:Hepatectomy / Q000379:methods*; D045022:Lactate Dehydrogenases / Q000097:blood; D008099:Liver / Q000378:metabolism*; D008163:Luminescent Measurements; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism; D018384:Oxidative Stress; D011169:Portal Vein / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances; D013997:Time Factors
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde; D045022:Lactate Dehydrogenases; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase