title: Healing efficacy of sea buckthorn (Hippophae rhamnoides L.) seed oil in an ovine burn wound model.
abstract: To investigate the efficacy of sea buckthorn (SBT) seed oil - a rich source of substances known to have anti-atherogenic and cardioprotective activity, and to promote skin and mucosa epithelization - on burn wound healing, five adult sheep were subjected to 3rd degree flame burns. Two burn sites were made on the dorsum of the sheep and the eschar was excised down to the fascia. Split-thickness skin grafts were harvested, meshed, and fitted to the wounds. The autograft was placed on the fascia and SBT seed oil was topically applied to one recipient and one donor site, respectively, with the remaining sites treated with vehicle. The wound blood flow (LASER Doppler), and epithelization (ultrasound) were determined at 6, 14, and 21 days after injury. 14 days after grafting, the percentage of epithelization in the treated sites was greater (95 ± 2.2% vs. 83 ± 2.9%, p<0.05) than in the untreated sites. Complete epithelization time was shorter in both treated recipient and donor sites (14.20 ± 0.48 vs. 19.60 ± 0.40 days, p<0.05 and 13.40 ± 1.02 vs. 19.60 ± 0.50 days, p<0.05, respectively) than in the untreated sites, confirmed by ultrasound. In conclusion, SBT seed oil has significant wound healing activity in full-thickness burns and split-thickness harvested wounds.
authors.affiliation: Department of Anesthesiology, Investigational Intensive Care Unit, The University of Texas Medical Branch and Shriners Burns Hospital for Children, 601 Harborside Drive, Galveston, TX 77555-1102, USA; Department of Plastic and Reconstructive Surgery, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan. Electronic address: ihiroshi@coffee.ocn.ne.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ito
authors.affiliation: Department of Anesthesiology, Investigational Intensive Care Unit, The University of Texas Medical Branch and Shriners Burns Hospital for Children, 601 Harborside Drive, Galveston, TX 77555-1102, USA.
authors.forename: Sven
authors.initials: S
authors.lastname: Asmussen
authors.affiliation: Department of Anesthesiology, Investigational Intensive Care Unit, The University of Texas Medical Branch and Shriners Burns Hospital for Children, 601 Harborside Drive, Galveston, TX 77555-1102, USA.
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Traber
authors.affiliation: Department of Anesthesiology, Investigational Intensive Care Unit, The University of Texas Medical Branch and Shriners Burns Hospital for Children, 601 Harborside Drive, Galveston, TX 77555-1102, USA.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Cox
authors.affiliation: Department of Anesthesiology, Investigational Intensive Care Unit, The University of Texas Medical Branch and Shriners Burns Hospital for Children, 601 Harborside Drive, Galveston, TX 77555-1102, USA.
authors.forename: Hal K
authors.initials: HK
authors.lastname: Hawkins
authors.affiliation: Center for Electromechanics, University of Texas, 1 University Station PRC R7000, Austin, TX 78712, USA.
authors.forename: Rhykka
authors.initials: R
authors.lastname: Connelly
authors.affiliation: Department of Anesthesiology, Investigational Intensive Care Unit, The University of Texas Medical Branch and Shriners Burns Hospital for Children, 601 Harborside Drive, Galveston, TX 77555-1102, USA.
authors.forename: Lillian D
authors.initials: LD
authors.lastname: Traber
authors.affiliation: Department of Anesthesiology, Investigational Intensive Care Unit, The University of Texas Medical Branch and Shriners Burns Hospital for Children, 601 Harborside Drive, Galveston, TX 77555-1102, USA.
authors.forename: Timothy W
authors.initials: TW
authors.lastname: Walker
authors.affiliation: Department of Anesthesiology, Investigational Intensive Care Unit, The University of Texas Medical Branch and Shriners Burns Hospital for Children, 601 Harborside Drive, Galveston, TX 77555-1102, USA.
authors.forename: Erik
authors.initials: E
authors.lastname: Malgerud
authors.affiliation: Department of Plastic and Reconstructive Surgery, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sakurai
authors.affiliation: Department of Anesthesiology, Investigational Intensive Care Unit, The University of Texas Medical Branch and Shriners Burns Hospital for Children, 601 Harborside Drive, Galveston, TX 77555-1102, USA.
authors.forename: Perenlei
authors.initials: P
authors.lastname: Enkhbaatar
mesh_terms: D000818:Animals; D002056:Burns / Q000000981:diagnostic imaging / Q000601:surgery*; D003646:Debridement; D004195:Disease Models, Animal; D031281:Hippophae; D017078:Laser-Doppler Flowmetry; D008517:Phytotherapy; D010938:Plant Oils / Q000494:pharmacology*; D012639:Seeds; D034561:Sheep, Domestic; D016038:Skin Transplantation / Q000379:methods*; D014182:Transplantation, Autologous; D014463:Ultrasonography; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D010938:Plant Oils
keywords: Burn wounds; Sea buckthorn seed oil; Skin autograft