title: Extracellular enzyme activities of the monokaryotic strains generated from basidiospores of shiitake mushroom.
abstract: To obtain basic information on the biochemical property of basidiospores of shiitake mushroom (Lentinula edodes), the ability of producing extracellular enzyme was assessed using a chromogenic plate-based assay. For the aim, amylase, avicelase, β-glucosidase, CM-cellulase, pectinase, proteinase, and xylanase were tested against monokaryotic strains generated from forty basidiospores of two different parental dikaryotic strains of shiitake mushroom, Sanjo-101Ho and Sanjo-108Ho. These two parental strains showed different degree of extracellular enzyme activity. No identical patterns of the degree of enzyme activity were observed between monokaryotic strains and parental strains of the two shiitake cultivars. The degree of extracellular enzyme activity also varied among monokaryotic strains of the two shiitake cultivars. Our results showed that dikaryotic parental strains of shiitake mushroom produce monokaryotic basidiospores having very diverse biochemical properties.
authors.affiliation: Department of Microbiology and Institute of Basic Sciences, Dankook University, Cheonan, Chungnam 330-714, Korea.
authors.forename: Hyuk Woo
authors.initials: HW
authors.lastname: Kwon
authors.forename: In Joung
authors.initials: IJ
authors.lastname: Back
authors.forename: Han Gyu
authors.initials: HG
authors.lastname: Ko
authors.forename: Chang Hyun
authors.initials: CH
authors.lastname: You
authors.forename: Seong Hwan
authors.initials: SH
authors.lastname: Kim
keywords: Basidiospores; Chromogenic media; Extracellular enzyme activity; Shiitake mushroom