title: Investigation of interaction between magnetic silica particles and lambda phage DNA fragment.
abstract: Nucleic acids belong to the most important molecules and therefore the understanding of their properties, function and behavior is crucial. Even though a range of analytical and biochemical methods have been developed for this purpose, one common step is essential for all of them - isolation of the nucleic acid from the from complex sample matrix. The use of magnetic particles for the separation of nucleic acids has many advantages over other isolation methods. In this study, an isolation procedure for extraction of DNA was optimized. Each step of the isolation process including washing, immobilization and elution was optimized and therefore the efficiency was increased from 1.7% to 28.7% and the total time was shortened from 75 to 30min comparing to the previously described method. Quantification of the particular parameter influence was performed by square-wave voltammetry using hanging drop mercury electrode. Further, we compared the optimized method with standard chloroform extraction and applied on isolation of DNA from Staphylococcus aureus and Escherichia coli.
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic.
authors.forename: Kristyna
authors.initials: K
authors.lastname: Smerkova
authors.forename: Simona
authors.initials: S
authors.lastname: Dostalova
authors.forename: Marketa
authors.initials: M
authors.lastname: Vaculovicova
authors.forename: Jindrich
authors.initials: J
authors.lastname: Kynicky
authors.forename: Libuse
authors.initials: L
authors.lastname: Trnkova
authors.forename: Miroslav
authors.initials: M
authors.lastname: Kralik
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
authors.forename: Jaromir
authors.initials: J
authors.lastname: Hubalek
authors.forename: Ivo
authors.initials: I
authors.lastname: Provaznik
authors.forename: Rene
authors.initials: R
authors.lastname: Kizek
mesh_terms: D010582:Bacteriophage lambda / Q000378:metabolism*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004267:DNA Viruses / Q000378:metabolism; D060328:Magnetic Phenomena; D010316:Particle Size; D012822:Silicon Dioxide / Q000032:analysis* / Q000378:metabolism*
chemical_list: D012822:Silicon Dioxide
keywords: DNA isolation; Lambda phage; Magnetic particles; Polymerase chain reaction; Voltammetry