title: [Screening of the gene mutation in D-loop region of mitochondrial DNA in oral squamous cell carcinoma].
abstract: OBJECTIVE
  To investigate the gene mutation in D-loop region of mitochondrial DNA (mtDNA) in oral squamous cell carcinoma (OSCC) tissue and to explore the role of the gene mutation in D-loop region in the OSCC tumorigenesis.
  
  
  METHODS
  mtDNA was obtained from cancer, paracancerous and normal mucosa tissues of thirty patients with OSCC. The D-loop regions of mtDNA were amplified with PCR, sequencing and then analyzed by Chromas software and BLAST to identify the mutation site.
  
  
  RESULTS
  Mutation in the D-loop region was found in eight cases, with the mutation rate of 27%. There were nine mutations totally, including one point mutation, two base deletions, three insertion mutations, three heterozygous mutations. In these mutations, base deletions were different from each other and heterozygous mutations had no same mutation form, while the three insertion mutations were same, the insertion of base C. One case had T/A heterozygous mutation and base C insertion at the same time.
  
  
  CONCLUSIONS
  There were mutations in mtDNA D-loop in OSCC, but the relationship between occurrence of OSCC and mutation of mtDNA needs further study.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Qingdao University, Qingdao Shandong, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Sun
authors.forename: Rong-tao
authors.initials: RT
authors.lastname: Yuan
authors.forename: Wan-tao
authors.initials: WT
authors.lastname: Chen
authors.forename: Ling-xue
authors.initials: LX
authors.lastname: Bu
authors.forename: Mu-yun
authors.initials: MY
authors.lastname: Jia
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics*; D009154:Mutation
chemical_list: D004272:DNA, Mitochondrial