title: Exploratory investigation reveals parallel alteration of plasma fatty acids and eicosanoids in coronary artery disease patients.
abstract: Fatty acids and eicosanoids are two important classes of signaling lipid molecules involved in the pathogenesis of cardiovascular diseases. To investigate the physiological functions and interplay between fatty acids and eicosanoids in coronary artery disease (CAD) patients, we developed an analytical approach for parallel quantitative analysis of plasma fatty acids and eicosanoids, using gas chromatography-tandem mass spectrometry (GC-MS/MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). In this study, 26 fatty acids and 12 eicosanoids were confidently detected in 12 patients with confirmed coronary artery disease and 11 healthy subjects. Pattern recognition analysis (principal components analysis, orthogonal partial least-square discriminate analysis, and hierarchical clustering analysis) demonstrated that the plasma lipid profile of fatty acids and eicosanoids enabled robust discrimination of CAD patients versus healthy subjects. Significant differences in six fatty acids and five eicosanoids were noted among CAD patients and healthy subjects. The development of cardiovascular disease-induced metabolic change of fatty acids and eicosanoids, such as eicosapentaenoic acid, docosahexaenoic acid, arachidonic acid, hydroxyeicosatetraenoic acids and hydroxyoctadecadienoic acid, were consistent with previous isolated observations. Moderate-strong correlations between three plasma fatty acids and three eicosanoids from arachidonic acid metabolism were also observed. In brief, findings from this exploratory study offered a new insight on the roles of various bioactive lipid molecules in the development of coronary artery disease biomarkers.
authors.affiliation: Saw Swee Hock School of Public Health, National University of Singapore, Singapore; Key Laboratory of Insect Development and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Science, Chinese Academy of Sciences, Shanghai, People's Republic of China. Electronic address: yjxutju@gmail.com.
authors.forename: Yong-Jiang
authors.initials: YJ
authors.lastname: Xu
authors.forename: Wanxing Eugene
authors.initials: WE
authors.lastname: Ho
authors.forename: Fengguo
authors.initials: F
authors.lastname: Xu
authors.forename: Tao
authors.initials: T
authors.lastname: Wen
authors.forename: Choon Nam
authors.initials: CN
authors.lastname: Ong
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000097:blood*; D015777:Eicosanoids / Q000097:blood*; D005227:Fatty Acids / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis
chemical_list: D015777:Eicosanoids; D005227:Fatty Acids
keywords: Cardiovascular diseases; Eicosanoids; Fatty acids; GC/MS; LC/MS; Lipids