title: [Effects of survivin gene silencing by small interfering RNA on apoptosis of oral squamous cell carcinoma].
abstract: OBJECTIVE
  To apply the small interfering RNA (siRNA) targeting survivin to inhibit expression of survivin gene in HSC-3 oral squamous cell carcinoma and to increase the apoptosis of HSC-3 cells.
  
  
  METHODS
  siRNA was synthesized and transfected into oral squamous cell carcinoma HSC-3 cells. Compared with negative control group and blank control group, semi-quantitive reverse transcription-polymerase chain reaction (RT-PCR) was used to detect survivin mRNA. Cellular viability was detected by MTT and HSC-3 cell apoptosis was determined by tunnel and flow cytometry.
  
  
  RESULTS
  Expression of survivin mRNA was decreased significantly in siRNA group compared with negative control group and blank control group. Cellular viability was not affected in negative control group and blank control group, but cellular viability in siRNA group was significantly decreased. As to the cellular apoptosis rate, the transfected group (24.99% +/- 1.33%) was significantly higher than negative control group (1.24% +/- 0.13%) and blank control group (0.10% +/- 0.02%).
  
  
  CONCLUSIONS
  Survivin gene silenced by siRNA might promote apoptosis of oral squamous cell carcinoma. Survivin siRNA gene therapy would become a new target point for oral squamous cell carcinoma.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
authors.forename: Shifeng
authors.initials: S
authors.lastname: Liu
authors.forename: Xinhua
authors.initials: X
authors.lastname: Liang
authors.forename: Chongyun
authors.initials: C
authors.lastname: Bao
mesh_terms: D017209:Apoptosis; D002294:Carcinoma, Squamous Cell; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D020868:Gene Silencing; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009062:Mouth Neoplasms; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014162:Transfection
chemical_list: D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering