title: Low cytotoxic tissue adhesive based on oxidized dextran and epsilon-poly-L-lysine.
abstract: A novel adhesive hydrogel consisting of dextran and epsilon-poly(L-lysine) (dextran-PL) with multiple biomedical applications was developed. Periodate oxidation in aqueous media almost stoichiometrically introduces aldehyde groups in dextran molecules, and aldehyde dextran can react with the primary amino groups in epsilon-PL (ɛ-PL) at neutral pH to form a hydrogel. The gelation time of the hydrogel can be easily controlled by the extent of oxidation in dextran and of the acylation in ɛ-PL by anhydrides. The shear adhesion strength of dextran-PL was 10 times higher than that of fibrin glue, when wet collagen sheets were selected as test specimens. The cytotoxicity of aldehyde dextran and ɛ-PL were 1000 times lower than that of glutaraldehyde and poly(allylamine). The considerably low cytotoxicity of aldehyde dextran could be ascribed to its low reactivity with amine species when compared with glutaraldehyde. In contrast, a high reactivity of amino groups in ɛ-PL was observed when compared with glycine, L-lysine, and gelatin, which could be explained by their poor dissociation at neutral pH, thus leading to low cytotoxicity.
authors.affiliation: Center for Fiber and Textile Science, Kyoto Institute of Technology, Matsugasaki, Kyoto, 606-8585, Japan.
authors.forename: Suong-Hyu
authors.initials: SH
authors.lastname: Hyon
authors.forename: Naoki
authors.initials: N
authors.lastname: Nakajima
authors.forename: Hajime
authors.initials: H
authors.lastname: Sugai
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Matsumura
mesh_terms: D000215:Acylation; D000447:Aldehydes / Q000737:chemistry; D000812:Anhydrides / Q000737:chemistry; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D003911:Dextrans / Q000494:pharmacology*; D051379:Mice; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry / Q000494:pharmacology*; D066244:Proton Magnetic Resonance Spectroscopy; D033081:Shear Strength / Q000187:drug effects; D012996:Solutions; D013997:Time Factors; D014014:Tissue Adhesives / Q000633:toxicity*
chemical_list: D000447:Aldehydes; D000812:Anhydrides; D003911:Dextrans; D012996:Solutions; D014014:Tissue Adhesives; D010504:Periodic Acid; D011107:Polylysine; C009288:metaperiodate
keywords: adhesive; cytotoxicity; dextran; epsilon-poly(l-lysine); periodate