title: Kodamaea transpacifica f.a., sp. nov., a yeast species isolated from ephemeral flowers and insects in the Galapagos Islands and Malaysia: further evidence for ancient human transpacific contacts.
abstract: Twenty-four yeast strains were isolated from ephemeral flowers of Ipomoea spp. and Datura sp. and their associated insects in the Galápagos Archipelago, Ecuador, and from Ipomoea spp. and associated insects in the Cameron Highlands, Malaysia. Sequences of the D1/D2 domains of the large subunit rRNA gene indicated that these strains belong to a novel yeast species of the Kodamaea clade, although the formation of ascospores was not observed. The closest relative is Candida restingae. The human-mediated dispersion of this species by transpacific contacts in ancient times is suggested. The name Kodamaea transpacifica f.a., sp. nov. is proposed to accommodate these isolates. The type strain is CLQCA-24i-070(T) ( = CBS 12823(T) = NCYC 3852(T)); MycoBank number MB 803609.
authors.affiliation: Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil.
authors.forename: Larissa F D
authors.initials: LFD
authors.lastname: Freitas
authors.affiliation: Colección de Levaduras Quito Católica, Centro Neotropical para Investigación de la Biomasa, Pontificia Universidad Católica del Ecuador, Escuela de Ciencias Biológicas, CP 17-01-2184, Quito, Ecuador.
authors.forename: Enrique Javier Carvajal
authors.initials: EJC
authors.lastname: Barriga
authors.affiliation: Colección de Levaduras Quito Católica, Centro Neotropical para Investigación de la Biomasa, Pontificia Universidad Católica del Ecuador, Escuela de Ciencias Biológicas, CP 17-01-2184, Quito, Ecuador.
authors.forename: Patricia Portero
authors.initials: PP
authors.lastname: Barahona
authors.affiliation: Department of Biology, University of Western Ontario, London, Ontario N6A 5B7, Canada.
authors.forename: Marc-André
authors.initials: MA
authors.lastname: Lachance
authors.affiliation: Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Rosa
mesh_terms: D000818:Animals; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D004484:Ecuador; D035264:Flowers / Q000382:microbiology*; D006801:Humans; D007313:Insecta / Q000382:microbiology*; D027741:Ipomoea / Q000382:microbiology*; D008296:Malaysia; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D054683:Ribosome Subunits, Large, Eukaryotic / Q000235:genetics; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal