title: Sialic acid-binding lectin (leczyme) induces caspase-dependent apoptosis-mediated mitochondrial perturbation in Jurkat cells.
abstract: Sialic acid binding lectin (SBL) isolated from Rana catesbeiana oocytes is a multifunctional protein which has lectin activity, ribonuclease activity and antitumor activity. However, the mechanism of antitumor effects of SBL is unclear to date and the validity for human leukemia cells has not been fully studied. We report here that SBL shows cytotoxicity for some human leukemia cell lines including multidrug-resistant (MDR) cells. The precise mechanisms of SBL-induced apoptotic signals were analyzed by combinational usage of specific caspase inhibitors and the mitochondrial membrane depolarization detector JC-1. It was demonstrated that SBL causes mitochondrial perturbation and the apoptotic signal is amplified by caspases and cell death is executed in a caspase-dependent manner. The efficacy of this combinational usage was shown for the first time, to distinguish the apoptotic pathway in detail. SBL selectively kills tumor cells, is able to exhibit cytotoxicity regardless of P-glycoprotein expression and has potential as an alternative to conventional DNA-damaging anticancer drugs.
authors.affiliation: Division of Cell Recognition Study, Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University, Aoba-ku, Sendai 981-8558, Japan.
authors.forename: Takeo
authors.initials: T
authors.lastname: Tatsuta
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hosono
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sugawara
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kariya
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Ogawa
authors.forename: Senitiroh
authors.initials: S
authors.lastname: Hakomori
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nitta
mesh_terms: D029845:Amphibian Proteins / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D020169:Caspases / Q000235:genetics / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D019169:Jurkat Cells / Q000187:drug effects; D037102:Lectins / Q000494:pharmacology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D008928:Mitochondria / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012260:Ribonucleases / Q000494:pharmacology*
chemical_list: D029845:Amphibian Proteins; D037102:Lectins; D012333:RNA, Messenger; D012260:Ribonucleases; C448662:leczyme protein, Rana; D020169:Caspases