title: Structural templates for modeling homodimers.
abstract: Oligomeric proteins are more abundant in nature than monomeric proteins, and involved in all biological processes. In the absence of an experimental structure, their subunits can be modeled from their sequence like monomeric proteins, but reliable procedures to build the oligomeric assembly are scarce. Template-based methods, which start from known protein structures, are commonly applied to model subunits. We present a method to model homodimers that relies on a structural alignment of the subunits, and test it on a set of 511 target structures recently released by the Protein Data Bank, taking as templates the earlier released structures of 3108 homodimeric proteins (H-set), and 2691 monomeric proteins that form dimer-like assemblies in crystals (M-set). The structural alignment identifies a H-set template for 97% of the targets, and in half of the cases, it yields a correct model of the dimer geometry and residue-residue contacts in the target. It also identifies a M-set template for most of the targets, and some of the crystal dimers are very similar to the target homodimers. The procedure efficiently detects homology at low levels of sequence identities, and points to erroneous quaternary structures in the Protein Data Bank. The high coverage of the target set suggests that the content of the Protein Data Bank already approaches the structural diversity of protein assemblies in nature, and that template-based methods should become the choice method for modeling oligomeric as well as monomeric proteins.
authors.affiliation: Center for Bioinformatics, The University of Kansas, 2030 Becker Dr., Lawrence, Kansas, 66047.
authors.forename: Petras J
authors.initials: PJ
authors.lastname: Kundrotas
authors.forename: Ilya A
authors.initials: IA
authors.lastname: Vakser
authors.forename: Joël
authors.initials: J
authors.lastname: Janin
mesh_terms: D030562:Databases, Protein; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D011487:Protein Conformation; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
chemical_list: D011506:Proteins
keywords: protein modeling; quaternary structure; structural bioinformatics; structure alignment