title: Practical considerations over spectral quality in solid state NMR spectroscopy of soluble proteins.
abstract: Great theoretical and methodological advances are pushing the limits of resolution and sensitivity in solid state NMR (SSNMR). However, sample preparation remains a critical issue for the success of an experiment. The factors affecting spectral quality in SSNMR samples are discussed, examining cases encountered in the literature and presenting new experimental data. A discussion on resolution and sensitivity in sedimented solutes is framed in this context.
authors.affiliation: Center for Magnetic Resonance (CERM), University of Florence, Via L. Sacconi 6, 50019, Sesto Fiorentino, FI, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Fragai
authors.forename: Claudio
authors.initials: C
authors.lastname: Luchinat
authors.forename: Giacomo
authors.initials: G
authors.lastname: Parigi
authors.forename: Enrico
authors.initials: E
authors.lastname: Ravera
mesh_terms: D003460:Crystallization; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular; D011506:Proteins / Q000737:chemistry*; D012995:Solubility; D013482:Superoxide Dismutase / Q000737:chemistry; D013696:Temperature; D025801:Ubiquitin / Q000737:chemistry; D014461:Ultracentrifugation
chemical_list: D011506:Proteins; D025801:Ubiquitin; D013482:Superoxide Dismutase