title: [Simultaneous determination of melamine and creatinine in milk and milk products by gas chromatography-mass spectrometry].
abstract: A method established for determining melamine and creatinine in milk and milk products. The samples were extracted with 1% trichloracetic acid solution. The extract was cleaned-up by using a mixed-mode cation ion exchange SPE column and evaporated to dryness under nitrogen. The residue was derived by bis (trimethylsilyl) trifluoroacetamide-chlorotrimethylsilane (BSTFA-TMCS), then determined by gas chromatography-mass spectrometry (GC-MS) in selected ion monitoring (SIM) mode. Melamine and creatinine had good linearity in the range of 0.1 - 50 mg/L with the correlation coefficients higher than 0.99, and the limits of quantification were 0.10 mg/kg and 0.20 mg/kg, respectively. The recoveries of melamine and creatinine were 80.7% - 116.8% and 77.6% - 107.5% with RSD no more than 9.4% and 8.5% in the spiked range of 10 - 100 mg/kg and 0.1 - 5.0 mg/kg, respectively. The method is accurate, sensitive, and suitable for the determination of creatinine in milk and milk products.
authors.affiliation: Nanchang Food Quality Health Safety Supervision and Inspection Center, Nanchang 330012, China. zk19831125@163.com
authors.forename: Kai
authors.initials: K
authors.lastname: Zeng
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Liu
authors.forename: Yajun
authors.initials: Y
authors.lastname: Ning
authors.forename: Jinlin
authors.initials: J
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000818:Animals; D003404:Creatinine / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D008892:Milk / Q000737:chemistry*; D014227:Triazines / Q000032:analysis*
chemical_list: D014227:Triazines; D003404:Creatinine; C011907:melamine