title: Immunohistochemical and morphometric analysis of immunoglobulin light-chain immunoreactive amyloid in psammoma bodies of the human choroid plexus.
abstract: The aim of this research was to establish the presence of amyloid and to quantify immunohistochemical reactions of kappa and lambda light chains of psammoma bodies of the choroid plexus. Choroid plexus tissue obtained from 14 right lateral ventricles postmortem was processed histologically and stained with Congo red, thioflavin T, and monoclonal antibodies for kappa and lambda light chains. Morphological analysis was performed with a light microscope at lens magnifications of 4×, 10×, 20×, 25×, and 40×. The morphometric characteristics of psammoma bodies that were kappa and lambda positive and negative were analyzed with ImageJ. Histological analysis showed that the psammoma bodies, stromal blood vessel walls, and some epithelial cells reacted positively with Congo red and thioflavin T. Psammoma bodies were predominantly positive for lambda light chains. Lambda positivity was detected inside some stromal blood vessels, which pointed to a probable systemic origin for these light chains. Morphometric analysis showed that the mean optical densities of lambda- and kappa-positive psammoma bodies were significantly higher than those that gave a negative reaction. The percentage of lambda-positive psammoma bodies was significantly higher than the percentage of lambda-negative psammoma bodies in 80% of the cases, while the reaction with kappa light chains was negative in the majority of the cases. Linear regression analysis showed a significant increase in the percentage of lambda-positive psammoma bodies and their mean optical density with age. Finally, it can be concluded that the positive reaction of psammoma bodies in the choroid plexus with respect to amyloid and lambda light chains may point to the presence of light-chain amyloid in their structures.
authors.affiliation: Department of Anatomy, Medical Faculty, University of Niš, Dr Zorana Djindjića 81, Niš, Serbia, ivanjov@sbb.rs.
authors.forename: Ivan
authors.initials: I
authors.lastname: Jovanović
authors.forename: Sladjana
authors.initials: S
authors.lastname: Ugrenović
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Vasović
authors.forename: Ivan
authors.initials: I
authors.lastname: Stojanović
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000682:Amyloid / Q000032:analysis*; D002831:Choroid Plexus / Q000033:anatomy & histology / Q000737:chemistry*; D005260:Female; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000032:analysis*; D007146:Immunoglobulin lambda-Chains / Q000032:analysis*; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged
chemical_list: D000682:Amyloid; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains