title: Protective effect of N-acetylserotonin against acute hepatic ischemia-reperfusion injury in mice.
abstract: The purpose of this study was to investigate the possible protective effect of N-acetylserotonin (NAS) against acute hepatic ischemia-reperfusion (I/R) injury in mice. Adult male mice were randomly divided into three groups: sham, I/R, and I/R + NAS. The hepatic I/R injury model was generated by clamping the hepatic artery, portal vein, and common bile duct with a microvascular bulldog clamp for 30 min, and then removing the clamp and allowing reperfusion for 6 h. Morphologic changes and hepatocyte apoptosis were evaluated by hematoxylin-eosin (HE) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, respectively. Activated caspase-3 expression was evaluated by immunohistochemistry and Western blot. The activation of aspartate aminotransferase (AST), malondialdehyde (MDA), and superoxide dismutase (SOD) was evaluated by enzyme-linked immunosorbent assay (ELISA). The data show that NAS rescued hepatocyte morphological damage and dysfunction, decreased the number of apoptotic hepatocytes, and reduced caspase-3 activation. Our work demonstrates that NAS ameliorates hepatic IR injury.
authors.affiliation: Departments of Anatomy, Weifang Medical University, Weifang 261053, China.
authors.forename: Shuna
authors.initials: S
authors.lastname: Yu
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
authors.forename: Zhengchen
authors.initials: Z
authors.lastname: Jiang
authors.forename: Caixing
authors.initials: C
authors.lastname: Shi
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Du
authors.forename: Hailiang
authors.initials: H
authors.lastname: Wang
authors.forename: Jiying
authors.initials: J
authors.lastname: Jiang
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001219:Aspartate Aminotransferases / Q000097:blood; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008099:Liver / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism*; D012701:Serotonin / Q000031:analogs & derivatives* / Q000627:therapeutic use; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D012701:Serotonin; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D053148:Caspase 3; C006389:N-acetylserotonin