title: Protective effect of 3,4-methylenedioxyphenol (sesamol) on stress-related mucosal disease in rats.
abstract: Stress-related mucosal disease (SRMD) causes considerable morbidity and mortality in critically ill patients. 3,4-Methylenedioxyphenol (sesamol) has been reported to have potent antioxidative and anti-inflammatory properties. The aim of this study was to investigate the effect of sesamol on water immersion restraint- (WIR-) induced SRMD in rats. Rat gastric ulcer and hemorrhage were induced by WIR. Rats were pretreated orally with various doses of sesamol (0.1, 0.3, and 1 mg/kg, resp.) 30 min before WIR. Gastric mucosal ulceration, hemoglobin, lipid peroxidation, mucus secretion, proinflammatory cytokines, and nuclear factor (NF)-κB levels were determined 4 h after WIR. In addition, the infiltration of neutrophil and macrophage into gastric mucosa was also determined after WIR. Water immersion restraint increased gastric mucosal ulcer and hemorrhage, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 levels but failed to affect mucosal lipid peroxidation and mucus secretion compared with non-WIR. Sesamol significantly decreased gastric ulceration and hemorrhage and inhibited mucosal TNF-α, IL-1β, and IL-6 production and NF-κB activity in WIR-treated rats. In addition, increased myeloperoxidase and CD68 levels in gastric mucosa were found in WIR-treated rats compared to non-WIR rats. Sesamol did not affect myeloperoxidase but decreased CD68 levels in mucosa in WIR-treated rats. Sesamol may protect against SRMD by inhibiting gastric mucosal proinflammatory cytokines in rats.
authors.affiliation: Department of Environmental and Occupational Health, National Cheng Kung University Medical College, Tainan 70428, Taiwan.
authors.forename: Dur-Zong
authors.initials: DZ
authors.lastname: Hsu
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Chen
authors.forename: Pei-Yi
authors.initials: PY
authors.lastname: Chu
authors.forename: Srinivasan
authors.initials: S
authors.lastname: Periasamy
authors.forename: Ming-Yie
authors.initials: MY
authors.lastname: Liu
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D052117:Benzodioxoles / Q000494:pharmacology* / Q000627:therapeutic use*; D016207:Cytokines / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects / Q000473:pathology; D007101:Immersion; D018836:Inflammation Mediators / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000473:pathology; D008297:Male; D009093:Mucus / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D020556:Neutrophil Infiltration / Q000187:drug effects; D010636:Phenols / Q000494:pharmacology* / Q000627:therapeutic use*; D020011:Protective Agents / Q000494:pharmacology* / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical; D013276:Stomach Ulcer / Q000188:drug therapy* / Q000209:etiology* / Q000473:pathology; D013315:Stress, Psychological / Q000150:complications*; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D052117:Benzodioxoles; D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; D010636:Phenols; D020011:Protective Agents; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C025583:sesamol