title: Detection of plant oil DNA using high resolution melting (HRM) post PCR analysis: a tool for disclosure of olive oil adulteration.
abstract: Extra virgin olive oil is frequently subjected to adulterations with addition of oils obtained from plants other than olive. DNA analysis is a fast and economic tool to identify plant components in oils. Extraction and amplification of DNA by PCR was tested in olives, in milled seeds and in oils, to investigate its use in olive oil traceability. DNA was extracted from different oils made of hazelnut, maize, sunflower, peanut, sesame, soybean, rice and pumpkin. Comparing the DNA melting profiles in reference plant materials and in the oils, it was possible to identify any plant components in oils and mixtures of oils. Real-Time PCR (RT-PCR) platform has been added of the new methodology of high resolution melting (HRM), both were used to analyse olive oils mixed with different percentage of other oils. Results showed HRM a cost effective method for efficient detection of adulterations in olive oils.
authors.affiliation: SITEIA.PARMA Interdepartmental Centre, University of Parma, Parco Area Delle Scienze 181/A, 43124 Parma, Italy.
authors.forename: Michelangelo
authors.initials: M
authors.lastname: Vietina
authors.forename: Caterina
authors.initials: C
authors.lastname: Agrimonti
authors.forename: Nelson
authors.initials: N
authors.lastname: Marmiroli
mesh_terms: D018744:DNA, Plant / Q000737:chemistry* / Q000235:genetics; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D031658:Olea / Q000737:chemistry* / Q000235:genetics; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D044366:Transition Temperature
chemical_list: D018744:DNA, Plant; D000069463:Olive Oil; D010938:Plant Oils
keywords: Adulteration; DNA extraction; Olive oil; PCR high resolution melting (HRM); Seeds oil; Traceability