title: Fed-batch production of MCL-PHA with elevated 3-hydroxynonanoate content.
abstract: With no inhibition of β-oxidation, Pseudomonas putida KT2440 produces medium-chain-length poly(3-hydroxyalkanoate) (MCL-PHA) with approximately 65 mol% 3-hydroxynonanoate (HN) from nonanoic acid. Production of PHA with higher HN content and an adjustable monomeric composition was obtained using acrylic acid, a fatty acid β-oxidation inhibitor, together with nonanoic acid and glucose as co-substrates in fed-batch fermentations. Different monomeric compositions were obtained by varying the feeding conditions to impose different specific growth rates and inhibitor feed concentrations. At a nonanoic acid: glucose: acrylic acid feed mass ratio of 1.25: 1: 0.05 and a specific growth rate of 0.15 h-1, 71.4 g L-1 biomass was produced containing 75.5% PHA with 89 mol% HN at a cumulative PHA productivity of 1.8 g L-1 h-1.
authors.affiliation: Chemical Engineering, Queen's University, Kingston, ON K7L 3N6, Canada. juliana.ramsay@chee.queensu.ca.
authors.forename: Xuan Jade
authors.initials: XJ
authors.lastname: Jiang
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Sun
authors.forename: Juliana A
authors.initials: JA
authors.lastname: Ramsay
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Ramsay