title: Induction of apoptosis by grape seed extract (Vitis vinifera) in oral squamous cell carcinoma.
abstract: Development of novel therapeutic modalities is crucial for the treatment of oral squamous cell carcinoma (OSCC). Recent scientific studies have been focused on herbal medicines as potent anti-cancer drug candidates. This study is the first to investigate the cytotoxic effects and the mechanism of cell death induced by grape seed extract (GSE) in oral squamous cell carcinoma (KB cells). MTT (3-(4,5-dimetylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) and trypan blue assays were performed in KB cells as well as human umbilical vein endothelial cells (HUVEC) were used to analyze the cytotoxic activity of GSE. Furthermore, the apoptosis-inducing action of the extract was determined by TUNEL, DNA fragmentation and cell death analysis. Statistical significance was determined by analysis of variance (ANOVA), followed by Duncan's test at a significance level of P≤0.05. The results showed apoptotic potential of GSE, confirmed by significant inhibition of cell growth and viability in a dose- and time- dependent manner without inducing damage to non-cancerous cell line HUVEC. The results of this study suggest that this plant contains potential bioactive compound(s) for the treatment of oral squamous cell carcinoma.
authors.affiliation: Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Tabriz University of Medical Sciences, Daneshgah street 51666-14766, Tabriz, Iran.
authors.forename: Amirala
authors.initials: A
authors.lastname: Aghbali
authors.forename: Sepideh Vosough
authors.initials: SV
authors.lastname: Hosseini
authors.forename: Abbas
authors.initials: A
authors.lastname: Delazar
authors.forename: Nader Kalbasi
authors.initials: NK
authors.lastname: Gharavi
authors.forename: Fatemeh Zare
authors.initials: FZ
authors.lastname: Shahneh
authors.forename: Mona
authors.initials: M
authors.lastname: Orangi
authors.forename: Ali
authors.initials: A
authors.lastname: Bandehagh
authors.forename: Behzad
authors.initials: B
authors.lastname: Baradaran
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D053938:DNA Fragmentation; D004354:Drug Screening Assays, Antitumor; D056604:Grape Seed Extract / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D009062:Mouth Neoplasms / Q000473:pathology*; D027843:Vitis / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D056604:Grape Seed Extract