title: Emergence of new variants of ST131 clonal group among extraintestinal pathogenic Escherichia coli producing extended-spectrum β-lactamases.
abstract: Having shown that Lucus Augusti Hospital in Lugo, Spain, has been affected by Escherichia coli clone O25:H4-ST131 producing CTX-M-15, the present study was carried out to evaluate the prevalence of this clone among the extended-spectrum β-lactamase (ESBL)-producing E. coli isolates and to identify novel variants of this clone. Of the 77 ESBL-producing E. coli isolated between January and April 2012, 47 (61%) were identified as belonging to the ST131 clonal group, comprising 38 O25b:H4-B2-ST131 (34 CTX-M-15, 2 CTX-M-14, 1 CTX-M-1 and 1 CTX-M-27), 7 O-non-typeable:H4-B2-ST131 (all CTX-M-15) and 2 O16:H5-B2-ST131 (both CTX-M-14). The 47 isolates of ST131 exhibited a significantly higher virulence score (mean of 9.1 virulence genes) compared with the 30 non-ST131 isolates (mean of 4.3 virulence genes). A new virulence profile (fimH, papG II, sat, cnf1, hlyA, iucD, kpsM II-K5, traT, malX, usp) was detected among O25b:H4-B2-ST131 isolates belonging to the new Pasteur sequence type PST621. To our knowledge, this is the first study to report the O-non-typeable:H4-B2-ST131 and O16:H5-B2-ST131 variants in Europe.
authors.affiliation: Escherichia coli Reference Laboratory (LREC), Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Calle Carballo Calero s/n, 27002 Lugo, Spain.
authors.forename: Ghizlane
authors.initials: G
authors.lastname: Dahbi
authors.forename: Azucena
authors.initials: A
authors.lastname: Mora
authors.forename: Cecilia
authors.initials: C
authors.lastname: López
authors.forename: María Pilar
authors.initials: MP
authors.lastname: Alonso
authors.forename: Rosalía
authors.initials: R
authors.lastname: Mamani
authors.forename: Juan
authors.initials: J
authors.lastname: Marzoa
authors.forename: Amparo
authors.initials: A
authors.lastname: Coira
authors.forename: Fernando
authors.initials: F
authors.lastname: García-Garrote
authors.forename: Julia María
authors.initials: JM
authors.lastname: Pita
authors.forename: David
authors.initials: D
authors.lastname: Velasco
authors.forename: Alexandra
authors.initials: A
authors.lastname: Herrera
authors.forename: Susana
authors.initials: S
authors.lastname: Viso
authors.forename: Jesús E
authors.initials: JE
authors.lastname: Blanco
authors.forename: Miguel
authors.initials: M
authors.lastname: Blanco
authors.forename: Jorge
authors.initials: J
authors.lastname: Blanco
mesh_terms: D004926:Escherichia coli / Q000145:classification* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology*; D006801:Humans; D017720:Molecular Epidemiology; D058889:Molecular Typing; D011446:Prospective Studies; D012703:Serotyping; D013030:Spain / Q000453:epidemiology; D037521:Virulence Factors / Q000235:genetics; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D037521:Virulence Factors; D001618:beta-Lactamases
keywords: CTX-M-15; ESBL; Escherichia coli; ST131