title: Determination of 4-bromo-2,5-dimethoxy-N-[(2-methoxyphenyl)methyl]-benzeneethanamine (25B-NBOMe) in serum and urine by high performance liquid chromatography with tandem mass spectrometry in a case of severe intoxication.
abstract: We present a case of 4-bromo-2,5-dimethoxy-N-[(2-methoxyphenyl)methyl]-benzeneethanamine (25B-NBOMe), an N-benzyl phenethylamines derivative, intoxication and a high performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) method for detection and quantification of 25B-NBOMe. A 19-year-old male was found unresponsive with generalized grand mal seizure activity. On the second day of hospitalization, a friend admitted that the patient used 'some unknown drug' called 25B. Serum and urine collected were sent to the Virginia Commonwealth University Medical Center Toxicology Laboratory for analysis. An HPLC-MS/MS method for the identification and quantification of 25B-NBOMe using 2-(2,5-dimethoxyphenyl)-N-(2-methoxybenzyl)ethanamine (25H-NBOMe) as the internal standard (ISTD) was developed. As this is a novel, single-case presentation, an assay validation was performed prior to testing to ensure the reliability of the analytical results. The serum and urine specimens were determined to contain 180 pg/ml and1900 pg/ml of 25B-NBOMe, respectively.
authors.affiliation: Departments of Pharmacology & Toxicology, Virginia Commonwealth University, Richmond, VA, USA.
authors.forename: Justin L
authors.initials: JL
authors.lastname: Poklis
authors.forename: Carol R
authors.initials: CR
authors.lastname: Nanco
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Troendle
authors.forename: Carl E
authors.initials: CE
authors.lastname: Wolf
authors.forename: Alphonse
authors.initials: A
authors.lastname: Poklis
mesh_terms: D000328:Adult; D000840:Anisoles / Q000097:blood* / Q000633:toxicity / Q000652:urine*; D002853:Chromatography, Liquid / Q000379:methods; D015198:Designer Drugs / Q000493:pharmacokinetics* / Q000633:toxicity; D006801:Humans; D008297:Male; D010627:Phenethylamines / Q000097:blood* / Q000633:toxicity / Q000652:urine*; D015203:Reproducibility of Results; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods; D055815:Young Adult
chemical_list: D000840:Anisoles; D015198:Designer Drugs; D010627:Phenethylamines; C000595926:2-(4-bromo-2,5-dimethoxyphenyl)-N-((2-methoxyphenyl)methyl)ethanamine
keywords: 25B-MBOMe; HPLC-MS/MS; bath salts; designer drugs; overdose