title: [Expression, crystallization and crystallographic study of the 1st IgV domain of human CD96].
abstract: CD96 (Tactile) is an adhesion receptor expressed mainly on activated T cells, NK cells. As a family member of the immunoglobulin-like cell receptor, CD96 consists of three immunoglobulin-like domains (V1, V2/C and C) in the extracellular region. Recent studies have shown that the 1st IgV domain of CD96 (CD96V1) plays an essential role in cell adhesion and NK cell-mediated killing. In this study, the 1st IgV domain of human CD96 (hCD96V1) was cloned and expressed in Escherichia coli (BL21). The soluble protein was obtained by refolding of the hCD96V1 inclusion bodies. From analytical ultracentrifugation, we could predict that CD96 V1 maily exists as dimer with approximate molecular weight of 26.9 kDa. The protein was then successfully crystallized using the sitting-drop vapour-diffusion method. The crystals diffracted to 1.9 angstrom resolution and belonged to space group P21, with unit-cell parameters a = 35.1, b = 69.5, c = 49.6A, alpha=gamma=90 degrees, beta=105.4 degrees.
authors.affiliation: School of Life Science, Anhui Agricultural University, Hefei 230036, Anhui, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Jiang
authors.forename: Shuijun
authors.initials: S
authors.lastname: Zhang
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yan
authors.forename: Ning
authors.initials: N
authors.lastname: Guo
mesh_terms: D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics*; D003460:Crystallization; D003461:Crystallography / Q000379:methods; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis / Q000235:genetics*; D017434:Protein Structure, Tertiary / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D015703:Antigens, CD; C116131:CD96 antigen; D007135:Immunoglobulin Variable Region; D011994:Recombinant Proteins