title: The mitochondrial genome of Paramphistomum cervi (Digenea), the first representative for the family Paramphistomidae.
abstract: We determined the complete mitochondrial DNA (mtDNA) sequence of a fluke, Paramphistomum cervi (Digenea: Paramphistomidae). This genome (14,014 bp) is slightly larger than that of Clonorchis sinensis (13,875 bp), but smaller than those of other digenean species. The mt genome of P. cervi contains 12 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and 2 non-coding regions (NCRs), a complement consistent with those of other digeneans. The arrangement of protein-coding and ribosomal RNA genes in the P. cervi mitochondrial genome is identical to that of other digeneans except for a group of Schistosoma species that exhibit a derived arrangement. The positions of some transfer RNA genes differ. Bayesian phylogenetic analyses, based on concatenated nucleotide sequences and amino-acid sequences of the 12 protein-coding genes, placed P. cervi within the Order Plagiorchiida, but relationships depicted within that order were not quite as expected from previous studies. The complete mtDNA sequence of P. cervi provides important genetic markers for diagnostics, ecological and evolutionary studies of digeneans.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Key Laboratory of Veterinary Public Health of Agriculture Ministry, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, PR China.
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Yan
authors.forename: Xing-Ye
authors.initials: XY
authors.lastname: Wang
authors.forename: Zhong-Zi
authors.initials: ZZ
authors.lastname: Lou
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: David
authors.initials: D
authors.lastname: Blair
authors.forename: Hong
authors.initials: H
authors.lastname: Yin
authors.forename: Jin-Zhong
authors.initials: JZ
authors.lastname: Cai
authors.forename: Xue-Ling
authors.initials: XL
authors.lastname: Dai
authors.forename: Meng-Tong
authors.initials: MT
authors.lastname: Lei
authors.forename: Xing-Quan
authors.initials: XQ
authors.lastname: Zhu
authors.forename: Xue-Peng
authors.initials: XP
authors.lastname: Cai
authors.forename: Wan-Zhong
authors.initials: WZ
authors.lastname: Jia
mesh_terms: D000818:Animals; D001483:Base Sequence; D001499:Bayes Theorem; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics; D023061:Gene Order; D005819:Genetic Markers; D014644:Genetic Variation; D054629:Genome, Mitochondrial; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010250:Paramphistomatidae / Q000235:genetics*; D010802:Phylogeny; D012343:RNA, Transfer / Q000235:genetics; D022661:RNA, Untranslated / Q000235:genetics; D020539:Sequence Analysis, Protein
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D005819:Genetic Markers; D022661:RNA, Untranslated; D012343:RNA, Transfer