title: Phenolic profiles and antioxidant and anticarcinogenic activities of Greek herbal infusions; balancing delight and chemoprevention?
abstract: Total phenolic content, antioxidant activity and phenolic profiles of six herbal infusions - namely rosemary, Cretan dittany, St. John's Wort, sage, marjoram and thyme were assayed. Additionally, the infusion anticarcinogenic effect as to their ability to (a) scavenge free radicals, (b) inhibit cell growth, (c) decrease IL-8 levels and (d) regulate p65 subunit in epithelial colon cancer (HT29) and prostate (PC3) cancer cells was investigated. LC-DAD-MS and GC-MS analyses showed major qualitative and quantitative differences in phenolic profiles of the infusions. All herbal infusions exhibited antiradical activity which correlated strongly with their total phenolic content. Infusions exhibited the potential to inhibit cell growth and to reduce IL-8 levels in HT29 colon and PC3 prostate cancer cells. The regulation reported in p65 subunit in HT29 treated with St John's Wort and in PC3 treated with thyme might point to the NF-κB as the molecular target underlying the effect of these infusions.
authors.affiliation: Department of Science of Dietetics-Nutrition, Harokopio University, Athens, Greece. akaliora@hua.gr
authors.forename: Andriana C
authors.initials: AC
authors.lastname: Kaliora
authors.forename: Dimitra A A
authors.initials: DA
authors.lastname: Kogiannou
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Kefalas
authors.forename: Issidora S
authors.initials: IS
authors.lastname: Papassideri
authors.forename: Nick
authors.initials: N
authors.lastname: Kalogeropoulos
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D018890:Chemoprevention; D029001:Herbal Medicine; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D016209:Interleukin-8; D016328:NF-kappa B; D010636:Phenols; D010936:Plant Extracts
keywords: 2-diphenyl-1-picryhydrazyl; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; Cell growth arrest; Colon cancer; DMEM; DPPH; Dulbecco’s modified Eagle’s medium; GC-MS; Herbal infusions; IL-8; Inflammation; LC-DAD-MS; MTT; NF-κB; Polyphenols; Prostate cancer; Terpenic acids; interleukin-8; nuclear factor-kappa B; p65 Subunit