title: A Novel Compound Rasatiol Isolated from Raphanus sativus Has a Potential to Enhance Extracellular Matrix Synthesis in Dermal Fibroblasts.
abstract: BACKGROUND
  The fibrous proteins of extracellular matrix (ECM) produced by dermal fibroblast contributes to the maintenance of connective tissue integrity.
  
  
  OBJECTIVE
  This study is carried out to identify the bioactive ingredient from natural products that enhances ECM production in dermal fibroblasts.
  
  
  METHODS
  Bioassay-directed fractionation was used to isolate the active ingredient from natural extracts. The effects of rasatiol (isolated from Raphanus sativus) on ECM production in primary cultured human dermal fibroblasts was investigated by enzyme linked immunosorbent assay and western blot analysis.
  
  
  RESULTS
  Rasatiol accelerated fibroblast growth in a dose-dependent manner and increased the production of type 1 collagen, fibronectin and elastin. Phosphorylation of p42/44 extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, and Akt was remarkably increased by rasatiol, indicating that enhanced ECM production is linked to the activation of intracellular signaling cascades.
  
  
  CONCLUSIONS
  These results indicate that rasatiol stimulates the fibrous components of ECM production, and may be applied to the maintenance of skin texture.
authors.affiliation: Department of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, Daejeon, Korea.
authors.forename: Seok-Seon
authors.initials: SS
authors.lastname: Roh
authors.forename: Seung-Bae
authors.initials: SB
authors.lastname: Park
authors.forename: Seong-Mo
authors.initials: SM
authors.lastname: Park
authors.forename: Byoung Wook
authors.initials: BW
authors.lastname: Choi
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Lee
authors.forename: Yul-Lye
authors.initials: YL
authors.lastname: Hwang
authors.forename: Chang Hun
authors.initials: CH
authors.lastname: Kim
authors.forename: Hyun-Ah
authors.initials: HA
authors.lastname: Jeong
authors.forename: Chang Deok
authors.initials: CD
authors.lastname: Kim
authors.forename: Jeung-Hoon
authors.initials: JH
authors.lastname: Lee
keywords: Extracellular matrix; Fibroblasts; Rasatiol