title: The impact of fermentation and in vitro digestion on formation angiotensin converting enzyme (ACE) inhibitory peptides from pea proteins.
abstract: Pea seeds were fermented by Lactobacillus plantarum 299v in monoculture under different time and temperature conditions and the fermented products were digested in vitro under gastrointestinal conditions. After fermentation and digestion ACE inhibitory activity was determined. In all samples after fermentation no ACE inhibitory activity was noted. Potentially antihypertensive peptides were released during in vitro digestion. The highest DH (68.62%) were noted for control sample, although the lowest IC50 value (0.19 mg/ml) was determined for product after 7 days fermentation at 22 °C. The hydrolysate characterised by the highest ACE inhibitory activity was separated on Sephadex G10 and two peptides fractions were obtained. The highest ACE inhibitory activity (IC50=64.04 μg/ml) for the first fraction was noted. This fraction was separated by HPLC and identified by LC-MS/MS and the sequence of peptide derived from pea proteins was determined as KEDDEEEEQGEEE.
authors.affiliation: Department of Biochemistry and Food Chemistry, University of Life Sciences, ul. Skromna 8, 20-704 Lublin, Poland. anka_jakubczyk@tlen.pl
authors.forename: Anna
authors.initials: A
authors.lastname: Jakubczyk
authors.forename: Monika
authors.initials: M
authors.lastname: Karaś
authors.forename: Barbara
authors.initials: B
authors.lastname: Baraniak
authors.forename: Marlena
authors.initials: M
authors.lastname: Pietrzak
mesh_terms: D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D004063:Digestion; D005285:Fermentation; D041981:Gastrointestinal Tract / Q000378:metabolism* / Q000382:microbiology; D006801:Humans; D048191:Lactiplantibacillus plantarum / Q000378:metabolism*; D008954:Models, Biological; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D010455:Peptides; D010940:Plant Proteins
keywords: ACE inhibitory activity; Biological peptides; Fermented pea seeds