title: Post-translational modifications and mass spectrometry detection.
abstract: In this review, we provide a comprehensive bibliographic overview of the role of mass spectrometry and the recent technical developments in the detection of post-translational modifications (PTMs). We briefly describe the principles of mass spectrometry for detecting PTMs and the protein and peptide enrichment strategies for PTM analysis, including phosphorylation, acetylation and oxidation. This review presents a bibliographic overview of the scientific achievements and the recent technical development in the detection of PTMs is provided. In order to ascertain the state of the art in mass spectrometry and proteomics methodologies for the study of PTMs, we analyzed all the PTM data introduced in the Universal Protein Resource (UniProt) and the literature published in the last three years. The evolution of curated data in UniProt for proteins annotated as being post-translationally modified is also analyzed. Additionally, we have undertaken a careful analysis of the research articles published in the years 2010 to 2012 reporting the detection of PTMs in biological samples by mass spectrometry.
authors.affiliation: Mass Spectrometry Centre, QOPNA, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: André M N
authors.initials: AMN
authors.lastname: Silva
authors.affiliation: Mass Spectrometry Centre, QOPNA, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: Rui
authors.initials: R
authors.lastname: Vitorino
authors.affiliation: Mass Spectrometry Centre, QOPNA, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: M Rosário M
authors.initials: MRM
authors.lastname: Domingues
authors.affiliation: School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7 ET, United Kingdom.
authors.forename: Corinne M
authors.initials: CM
authors.lastname: Spickett
authors.affiliation: Mass Spectrometry Centre, QOPNA, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal. Electronic address: p.domingues@ua.pt.
authors.forename: Pedro
authors.initials: P
authors.lastname: Domingues
mesh_terms: D000107:Acetylation; D000818:Animals; D006801:Humans; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D040901:Proteomics
keywords: Literature review; Mass spectrometry; Post-translational modifications