title: Association between polymorphisms in the renin-angiotensin-aldosterone system genes and essential hypertension in the Han Chinese population.
abstract: BACKGROUND
  Renin-angiotensin-aldosterone system (RAAS) is the most important endocrine blood pressure control mechanism in our body, genes encoding components of this system have been strong candidates for the investigation of the genetic basis of hypertension. However, previous studies mainly focused on limited polymorphisms, thus we carried out a case-control study in the Han Chinese population to systemically investigate the association between polymorphisms in the RAAS genes and essential hypertension.
  
  
  METHODS
  905 essential hypertensive cases and 905 normotensive controls were recruited based on stringent inclusion and exclusion criteria. All 41 tagSNPs within RAAS genes were retrieved from HapMap, and the genotyping was performed using the GenomeLab SNPstream Genotyping System. Logistic regression analysis, Multifactor dimensionality reduction (MDR), stratified analysis and crossover analysis were used to identify and characterize interactions among the SNPs and the non-genetic factors.
  
  
  RESULTS
  Serum levels of total cholesterol (TC) and triglyceride (TG), and body mass index (BMI) were significantly higher in the hypertensive group than in the control group. Of 41 SNPs genotyped, rs3789678 and rs2493132 within AGT, rs4305 within ACE, rs275645 within AGTR1, rs3802230 and rs10086846 within CYP11B2 were shown to associate with hypertension. The MDR analysis demonstrated that the interaction between BMI and rs4305 increased the susceptibility to hypertension. Crossover analysis and stratified analysis further indicated that BMI has a major effect, and rs4305 has a minor effect.
  
  
  CONCLUSIONS
  These novel findings indicated that together with non-genetic factors, these genetic variants in the RAAS may play an important role in determining an individual's susceptibility to hypertension in the Han Chinese.
authors.affiliation: Department of Biochemistry, School of Medicine, Ningbo University, Ningbo, China ; State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.
authors.forename: Lindan
authors.initials: L
authors.lastname: Ji
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Cai
authors.forename: Lina
authors.initials: L
authors.lastname: Zhang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Fei
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Jia
authors.initials: J
authors.lastname: Su
authors.forename: Lissy
authors.initials: L
authors.lastname: Lazar
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.forename: Yaping
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People; D015992:Body Mass Index; D002681:China / Q000453:epidemiology / Q000208:ethnology; D019405:Cytochrome P-450 CYP11B2 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics; D006801:Humans; D006973:Hypertension / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics*; D012084:Renin-Angiotensin System / Q000235:genetics*
chemical_list: C544065:AGTR1 protein, human; D044140:Receptor, Angiotensin, Type 1; D019405:Cytochrome P-450 CYP11B2; D007703:Peptidyl-Dipeptidase A