title: Biomarkers screening between preoperative and postoperative patients in pancreatic cancer.
abstract: OBJECTIVE
  To investigate discriminating protein patterns and potential biomarkers in serum samples between pre/postoperative pancreatic cancer patients and healthy controls.
  
  
  METHODS
  23 serum samples from PC patients (12 preoperative and 11 postoperative) and 76 from healthy controls were analyzed using matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique combined with magnetic beads-based weak cation-exchange chromatography (MB-WCX). ClinProTools software selected several markers that made a distinction between pancreatic cancer patients and healthy controls.
  
  
  RESULTS
  49 m/z distinctive peaks were found among the three groups, of which 33 significant peaks with a P < 0.001 were detected. Two proteins could distinguish the preoperative pancreatic cancer patients from the healthy controls. About 15 proteins may be potential biomarkers in assessment of pancreatic cancer resection.
  
  
  CONCLUSIONS
  MB-MALDI-TOF-MS method could generate serum peptidome profiles of pancreatic cancer and provide a new approach to identify potential biomarkers for diagnosis and prognosis of this malignancy.
authors.affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shanxi, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Li
authors.forename: Juan
authors.initials: J
authors.lastname: Yang
authors.forename: Qing-Yong
authors.initials: QY
authors.lastname: Ma
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wu
authors.forename: Chen
authors.initials: C
authors.lastname: Huang
authors.forename: Xu-Qi
authors.initials: XQ
authors.lastname: Li
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D021441:Carcinoma, Pancreatic Ductal / Q000097:blood / Q000175:diagnosis* / Q000601:surgery; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000601:surgery; D011184:Postoperative Period; D057234:Preoperative Period; D011379:Prognosis; D040901:Proteomics; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor