title: Study of hepatocellular function in the murine model following hepatic artery selective clamping.
abstract: OBJECTIVE
  To investigate the impact of selective hepatic artery clamping (SHAC) in hepatocellular function.
  
  
  METHODS
  Three groups of Wistar male rats were subjected to SHAC ischemia period of 60min: Group A continuous SHAC were subjected to SHAC ischemia period of 60min, Group B intermittent SHAC of 30min with 5min of reperfusion and Group C intermittent SHAC of 15min with 5min of reperfusion. Animals without SHAC were included-Group D. To evaluate hepatocellular function blood markers and hepatic extraction function (HEF) using 99mTc-mebrofenin were performed before and after surgery. Flow cytometry was used to analyze oxidative stress and cell viability.
  
  
  RESULTS
  A mortality rate of 7.6% in Group A was observed. HEF maintained normal values between the groups. Flow cytometry demonstrated no significant differences between the groups in viability, type of cell death as well as in the production of reactive oxygen species.
  
  
  CONCLUSIONS
  The selective hepatic artery clamping compared to other clamping techniques results on increased cell viability and decreased hepatocyte death. The SHAC is a potential alternative to decrease per-operative bleeding while maintaining hepatocellular function.
authors.affiliation: Faculty of Medicine, University of Coimbra, Surgery A, Surgery Department, CHUC, Centre of Investigation on Environment, Genetics and Oncobiology (CIMAGO), Coimbra, Portugal. jglrt@hotmail.com
authors.forename: José Guilherme
authors.initials: JG
authors.lastname: Tralhão
authors.forename: Ana Margarida
authors.initials: AM
authors.lastname: Abrantes
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Gonçalves
authors.forename: Emir
authors.initials: E
authors.lastname: Hoti
authors.forename: Mafalda
authors.initials: M
authors.lastname: Laranjo
authors.forename: Ricardo
authors.initials: R
authors.lastname: Martins
authors.forename: Bárbara
authors.initials: B
authors.lastname: Oliveiros
authors.forename: Dulce
authors.initials: D
authors.lastname: Cardoso
authors.forename: Ana Bela
authors.initials: AB
authors.lastname: Sarmento-Ribeiro
authors.forename: Maria Filomena
authors.initials: MF
authors.lastname: Botelho
authors.forename: Francisco
authors.initials: F
authors.lastname: Castro-Sousa
mesh_terms: D000818:Animals; D002470:Cell Survival; D003250:Constriction; D005434:Flow Cytometry; D006499:Hepatic Artery / Q000601:surgery*; D022781:Hepatocytes / Q000502:physiology*; D007511:Ischemia / Q000503:physiopathology; D008099:Liver / Q000098:blood supply* / Q000166:cytology*; D008297:Male; D023421:Models, Animal; D018384:Oxidative Stress; D010545:Peroxides / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D015424:Reperfusion; D013997:Time Factors
chemical_list: D010545:Peroxides; D017382:Reactive Oxygen Species