title: Comparative proteomics of primary and secondary lutoids reveals that chitinase and glucanase play a crucial combined role in rubber particle aggregation in Hevea brasiliensis.
abstract: Lutoids are specific vacuole-based organelles within the latex-producing laticifers in rubber tree Hevea brasiliensis. Primary and secondary lutoids are found in the primary and secondary laticifers, respectively. Although both lutoid types perform similar roles in rubber particle aggregation (RPA) and latex coagulation, they vary greatly at the morphological and proteomic levels. To compare the differential proteins and determine the shared proteins of the two lutoid types, a proteomic analysis of lutoid membranes and inclusions was performed, revealing 169 proteins that were functionally classified into 14 families. Biological function analysis revealed that most of the proteins are involved in pathogen defense, chitin catabolism, and proton transport. Comparison of the gene and protein changed patterns and determination of the specific roles of several main lutoid proteins, such as glucanase, hevamine, and hevein, demonstrated that Chitinase and glucanase appeared to play crucial synergistic roles in RPA. Integrative analysis revealed a protein-based metabolic network mediating pH and ion homeostasis, defense response, and RPA in lutoids. From these findings, we developed a modified regulation model for lutoid-mediated RPA that will deepen our understanding of potential mechanisms involved in lutoid-mediated RPA and consequent latex coagulation.
authors.affiliation: Ministry of Agriculture Key Laboratory of Biology and Genetic Resources of Rubber Tree, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences , Danzhou Hainan 571737, P. R. China.
authors.forename: Xuchu
authors.initials: X
authors.lastname: Wang
authors.forename: Minjing
authors.initials: M
authors.lastname: Shi
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Yueyi
authors.initials: Y
authors.lastname: Chen
authors.forename: Fuge
authors.initials: F
authors.lastname: Cai
authors.forename: Shixin
authors.initials: S
authors.lastname: Zhang
authors.forename: Limin
authors.initials: L
authors.lastname: Wang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Tong
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Tian
mesh_terms: D000704:Analysis of Variance; D015153:Blotting, Western; D002681:China; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D028482:Hevea / Q000201:enzymology / Q000235:genetics*; D008247:Lysosomes / Q000201:enzymology* / Q000235:genetics; D008565:Membrane Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D008954:Models, Biological; D040901:Proteomics / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012408:Rubber / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D008565:Membrane Proteins; D012408:Rubber; D006026:Glycoside Hydrolases