title: Cellular auxin homeostasis under high temperature is regulated through a sorting NEXIN1-dependent endosomal trafficking pathway.
abstract: High-temperature-mediated adaptation in plant architecture is linked to the increased synthesis of the phytohormone auxin, which alters cellular auxin homeostasis. The auxin gradient, modulated by cellular auxin homeostasis, plays an important role in regulating the developmental fate of plant organs. Although the signaling mechanism that integrates auxin and high temperature is relatively well understood, the cellular auxin homeostasis mechanism under high temperature is largely unknown. Using the Arabidopsis thaliana root as a model, we demonstrate that under high temperature, roots counterbalance the elevated level of intracellular auxin by promoting shootward auxin efflux in a PIN-FORMED2 (PIN2)-dependent manner. Further analyses revealed that high temperature selectively promotes the retrieval of PIN2 from late endosomes and sorts them to the plasma membrane through an endosomal trafficking pathway dependent on SORTING NEXIN1. Our results demonstrate that recycling endosomal pathway plays an important role in facilitating plants adaptation to increased temperature.
authors.affiliation: Cryobiofrontier Research Centre, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan.
authors.forename: Taiki
authors.initials: T
authors.lastname: Hanzawa
authors.forename: Kyohei
authors.initials: K
authors.lastname: Shibasaki
authors.forename: Takahiro
authors.initials: T
authors.lastname: Numata
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kawamura
authors.forename: Thierry
authors.initials: T
authors.lastname: Gaude
authors.forename: Abidur
authors.initials: A
authors.lastname: Rahman
mesh_terms: D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism; D017930:Genes, Reporter; D018522:Gravitropism; D006706:Homeostasis; D006358:Hot Temperature; D007210:Indoleacetic Acids / Q000032:analysis / Q000378:metabolism*; D009154:Mutation; D010937:Plant Growth Regulators / Q000032:analysis / Q000378:metabolism*; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000502:physiology; D021381:Protein Transport; D011993:Recombinant Fusion Proteins; D036226:Seedlings / Q000166:cytology / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction; D058305:Sorting Nexins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D011993:Recombinant Fusion Proteins; D058305:Sorting Nexins; C117013:AT5G57090 protein, Arabidopsis; C584750:SNX1 protein, Arabidopsis