title: Methods to study the role of sirtuins in genome stability.
abstract: One of the most important roles of Sirtuins is to ensure the maintenance of genome integrity under stress conditions. In this chapter, we provide a methodology to study this role of Sirtuins at two different levels: detection of genomic instability (with the Neutral Comet Assay) and study of Sirtuin dynamics in chromatin under stress conditions (by isolating insoluble chromatin fractions).
authors.affiliation: Chromatin Biology Laboratory, Cancer Epigenetics and Biology Program (PEBC), Bellvitge Biomedical Research Institute (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain.
authors.forename: Paloma
authors.initials: P
authors.lastname: Martínez-Redondo
authors.forename: Alejandro
authors.initials: A
authors.lastname: Vaquero
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D002843:Chromatin / Q000235:genetics*; D020552:Comet Assay / Q000379:methods*; D004249:DNA Damage / Q000235:genetics* / Q000528:radiation effects; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D042822:Genomic Instability; D051379:Mice; D018345:Mice, Knockout; D037761:Sirtuins / Q000502:physiology*
chemical_list: D002843:Chromatin; D037761:Sirtuins