title: [Analysis of extracellular proteome of Staphylococcus aureus 6 at the end of exponential growth phase].
abstract: OBJECTIVE
  Determine protein specter that Staphylococcus aureus synthesizes and secretes at early growth phase--the exponential phase.
  
  
  METHODS
  Proteins secreted by S. aureus strain 6 into cultivation medium at the end of exponential growth phase (4.5 hours) were studied. 11 proteins were identified by liquid chromatography--mass-spectrometry method.
  
  
  RESULTS
  Only in 3 of these proteins the presence of signal peptides was predicted, which indicates their extracellular localization; the rest of the proteins were localized predominantly in bacterial cytoplasm. 5 of 11 proteins function as enzymes of carbohydrate metabolism. Other extracellular proteins that could indicate its contamination with proteins from disrupted bacterial cells were not detected in S. aureus cultural liquid filtrate. It has been suggested that enzymes of carbohydrate metabolism can provide bacterial cells with energy necessary for passage from lag-phase into exponential growth phase. Superoxide dismutase enzyme probably provides the course of oxidation-reduction processes. Synthesis of other proteolytic enzymes and toxins is carried out at later stages of development of bacterial population. Immunization of mice with a mixture of 11 identified proteins showed their protective properties after infection by S. aureus 6 strain.
  
  
  CONCLUSIONS
  Based on the above-mentioned, the complex of isolated proteins may be perspective in development of a new strategy of prophylaxis and therapy of staphylococcus infections.
authors.forename: I M
authors.initials: IM
authors.lastname: Gruber
authors.forename: F B
authors.initials: FB
authors.lastname: Donenko
authors.forename: O M
authors.initials: OM
authors.lastname: Ignatova
authors.forename: E A
authors.initials: EA
authors.lastname: Astashkina
authors.forename: R K
authors.initials: RK
authors.lastname: Ziganshin
authors.forename: E A
authors.initials: EA
authors.lastname: Zariad'eva
authors.forename: I B
authors.initials: IB
authors.lastname: Semenova
authors.forename: E A
authors.initials: EA
authors.lastname: Kurbatova
authors.forename: L S
authors.initials: LS
authors.lastname: Cherkasova
authors.forename: O E
authors.initials: OE
authors.lastname: Tarasova
authors.forename: M V
authors.initials: MV
authors.lastname: Kiselevskiĭ
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D050260:Carbohydrate Metabolism; D002853:Chromatography, Liquid; D003470:Culture Media / Q000737:chemistry; D007114:Immunization; D013058:Mass Spectrometry; D051379:Mice; D021382:Protein Sorting Signals / Q000235:genetics; D020543:Proteome / Q000235:genetics / Q000276:immunology; D013203:Staphylococcal Infections / Q000276:immunology* / Q000382:microbiology / Q000401:mortality / Q000517:prevention & control*; D013211:Staphylococcus aureus / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D016019:Survival Analysis
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D021382:Protein Sorting Signals; D020543:Proteome