title: Validation (in-house and collaboratory) of the quantification method for ethyl carbamate in alcoholic beverages and soy sauce by GC-MS.
abstract: A method for ethyl carbamate (EC) determination in alcoholic beverages and soy sauce was developed by GC-MS. We adopted the diatomaceous earth solid-phase extraction (SPE) column and elution solvent of ethyl acetate/diethyl ether (5:95 v/v) for sample cleaning. The in-house validation showed the limit of quantification (LOQ) was 5.0 μg/kg. In the accuracy assay, the total average recovery for was 96.7%. The relative standard deviations (RSDs) were <5%. Subsequently, a collaborative trial was organized for the further validation. The RSDs for repeatability and reproducibility were 1.2-7.8% and 2.3-9.6% respectively. It indicated that the present method performed well in different laboratories.
authors.affiliation: Department of Ophthalmology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Huang
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Pan
authors.forename: Ping-Gu
authors.initials: PG
authors.lastname: Wu
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
authors.forename: Jian-Long
authors.initials: JL
authors.lastname: Han
authors.forename: Xiang-Hong
authors.initials: XH
authors.lastname: Shen
mesh_terms: D000434:Alcoholic Beverages / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D052616:Solid Phase Extraction; D045730:Soy Foods / Q000032:analysis*; D014520:Urethane / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D014520:Urethane
keywords: Collaboratory study; Ethyl carbamate; Fermented foods; GC-MS; Solid-phase extraction