title: A polymorphism in the resistin gene promoter and the risk of multiple sclerosis.
abstract: OBJECTIVE
  Multiple sclerosis (MS) is an inflammatory neurodegenerative disorder that inflammatory cytokines have been implicated in its immunopathogenesis. Resistin, a member of cysteine-rich secretory proteins family, identified with inflammatory properties in humans. To elucidate whether different genotypes of resistin are involved in MS pathogenesis, we compared serum levels of TNF-α, IL-1β, hs-CRP, and resistin levels in different genotypes of MS patients with relapsing remitting type and healthy subjects.
  
  
  METHODS
  IN a case-control study, 86 MS patients and 86 age- and sex-matched healthy subjects were enrolled. The age, gender distribution, and BMI of MS patientsand control group were similar. The serum levels of TNF-α, IL-1β, and Resistin were measured by ELISA. hs-CRP was measured by imunoturbidimetric method. After DNA extraction, the analysis of -420C/G SNP (rs1862513) was performed via PCR-RFLP method.
  
  
  RESULTS
  The resistin, TNF-α, IL1β, and hs-CRP levels were significantly higher in MS patients compared with control group. The distribution of "rs1862513" genotypes were not significant between MS and control groups. Although resistin and TNFα levels were higher in GG genotype carriers of both groups, but the difference was significant only in MS patient.
  
  
  CONCLUSIONS
  Resistin gene polymorphisms may modify the being susceptible to MS disease, which may cause through various levels of cytokines between genotypes.
authors.affiliation: Tehran University of Medical Sciences, Tehran, Iran - arashh@bu.edu.
authors.forename: A
authors.initials: A
authors.lastname: Hossein-Nezhad
authors.forename: F N
authors.initials: FN
authors.lastname: Varzaneh
authors.forename: K
authors.initials: K
authors.lastname: Mirzaei
authors.forename: S
authors.initials: S
authors.lastname: Emamgholipour
authors.forename: F N
authors.initials: FN
authors.lastname: Varzaneh
authors.forename: M A
authors.initials: MA
authors.lastname: Sahraian
mesh_terms: D000328:Adult; D000873:Anthracenes; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000032:analysis / Q000235:genetics; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood / Q000235:genetics*; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics*; D052243:Resistin / Q000097:blood* / Q000235:genetics*; D012306:Risk; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000235:genetics; D055815:Young Adult
chemical_list: C546739:AnOV compound; D000873:Anthracenes; D015415:Biomarkers; D053583:Interleukin-1beta; D052243:Resistin; D014409:Tumor Necrosis Factor-alpha; D002097:C-Reactive Protein