title: Biodegradation of Phenanthrene by Pseudomonas putida and a Bacterial Consortium in the Presence and in the Absence of a Surfactant.
abstract: This study describes the biodegradation of phenanthrene in aqueous media in the presence and in the absence of a surfactant, Brij 30. Biodegradations were performed using either Pseudomonas putida DSMZ 8368 or a bacterial consortium Pyr01 isolated from one PAHs-polluted site. P. putida degraded phenanthrene to form 1-hydroxy-2-naphthoic acid (1H2Na) as the major metabolite. LC-MS analysis revealed the production of complementary intermediates in the presence of Brij 30, showing intense ions at mass-to-charge ratios (m/z) 97 and 195. Higher phenanthrene biodegradation rate was obtained in the presence of Brij 30. Conversely, in the case of Pyr01consortium, the addition of Brij 30 (0.5 g L(-1)) had a negative effect on biodegradation: no phenanthrene biodegradation products were detected in the medium, whereas a production of several intermediates (m/z 97, 195 and 293) was obtained without surfactant. New results on phenanthrene metabolism by P. putida DSMZ 8368 and Pyr01 consortium in the presence and in the absence of Brij 30 we obtained. They confirm that the knowledge of the effect of a surfactant on bacterial cultures is crucial for the optimization of surfactant-enhanced PAHs biodegradation.
authors.affiliation: Laboratoire Réactions et Génie des Procédés-UPR CNRS 3349, Nancy-Université, ENSAIA-2, Avenue de la Forêt de Haye BP 172, 54505 Vandoeuvre-les-Nancy Cedex, France ; Computer Aided Processes Engineering Faculty, D. Mendeleyev University of Chemical Technology of Russia, ul. Geroev Panfilovcev 20, Moscow, 123480 Russia.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Pantsyrnaya
authors.forename: Stéphane
authors.initials: S
authors.lastname: Delaunay
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Goergen
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Guédon
authors.forename: Cédric
authors.initials: C
authors.lastname: Paris
authors.forename: Pascal
authors.initials: P
authors.lastname: Poupin
authors.forename: Elena
authors.initials: E
authors.lastname: Guseva
authors.forename: Joseph
authors.initials: J
authors.lastname: Boudrant
keywords: Biodegradation; Consortium; P. putida; Phenanthrene; Surfactant