title: Novel organization of the arginine catabolic mobile element and staphylococcal cassette chromosome mec composite island and its horizontal transfer between distinct Staphylococcus aureus genotypes.
abstract: In this study, 425 methicillin-resistant Staphylococcus aureus (MRSA) isolates recovered in the Dutch-German Euregio were investigated for the presence of the arginine catabolic mobile element (ACME). Sequence analysis by whole-genome sequencing revealed an entirely new organization of the ACME-staphylococcal cassette chromosome mec composite island (SCCmec-CI), with truncated ACME type II located downstream of SCCmec. An identical nucleotide sequence of ACME-SCCmec-CI was found in two distinct MRSA lineages (t064-ST8 and t002-ST5), which has not been reported previously in S. aureus.
authors.affiliation: Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
authors.forename: Artur J
authors.initials: AJ
authors.lastname: Sabat
authors.forename: Robin
authors.initials: R
authors.lastname: Köck
authors.forename: Viktoria
authors.initials: V
authors.lastname: Akkerboom
authors.forename: Ron
authors.initials: R
authors.lastname: Hendrix
authors.forename: Robert L
authors.initials: RL
authors.lastname: Skov
authors.forename: Karsten
authors.initials: K
authors.lastname: Becker
authors.forename: Alexander W
authors.initials: AW
authors.lastname: Friedrich
mesh_terms: D001120:Arginine / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004251:DNA Transposable Elements; D022761:Gene Transfer, Horizontal; D016680:Genome, Bacterial; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D013203:Staphylococcal Infections / Q000382:microbiology
chemical_list: D004251:DNA Transposable Elements; D001120:Arginine