title: Cloning, expression, purification and preliminary X-ray analysis of the dimerization domain of ethylene response sensor 1 (ERS1) from Arabidopsis thaliana.
abstract: Ethylene signalling is initiated by a group of membrane-bound receptors with similarity to two-component systems. ERS1 belongs, together with ETR1, to subfamily 1, which plays a predominant role in ethylene signalling. The dimerization domain of ERS1 was crystallized in space groups C222(1) and P2(1)2(1)2, with two and four molecules per asymmetric unit, respectively. The crystals diffracted X-ray radiation to 1.9 Å resolution.
authors.affiliation: EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, 22603 Hamburg, Germany.
authors.forename: Hubert
authors.initials: H
authors.lastname: Mayerhofer
authors.forename: Jochen
authors.initials: J
authors.lastname: Mueller-Dieckmann
mesh_terms: D017360:Arabidopsis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics; D005030:Ethylenes / Q000378:metabolism; D015870:Gene Expression; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; C517775:ERS1 protein, Arabidopsis; D005030:Ethylenes; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; C036216:ethylene
keywords: Arabidopsis thaliana; ethylene response sensor 1 (ERS1)