title: Paclitaxel loaded fibrinogen coated CdTe/ZnTe core shell nanoparticles for targeted imaging and drug delivery to breast cancer cells.
abstract: The study aims at the targeted imaging using CdTe/ZnTe core shell QDs and delivery of paclitaxel (PTX) loaded fibrinogen coated yellow-QDs (PTX-fib-yellow-QDs) towards breast cancer cells via the alpha5Beta1-integrins. We developed fibrinogen coated different sized CdTe/ZnTe core shell quantum dots of 2-10 nm size, which have been prepared by one-pot aqueous-phase approach. The fib-coated-QDs (fib-coated-QDs) and PTX-fib-yellow-QDs were prepared by two-step coacervation technique using CaCl2 as cross-linker. Particle size of fib-coated-QDs was in between 60-220 nm while PTX-fib-yellow-QDs showed 180 +/- 40 nm. The MTT assay confirmed cytocompatibility of fib-coated-QDs on L929 and MCF-7 than bare QDs, whereas significant toxicity toward MCF-7 by PTX-fib-yellow-QDs was observed. The hemocompatible fib-coated-QDs showed enhanced localization and retention toward alpha5beta1-integrins +ve MCF-7 compared to alpha5beta1-integrins -ve L929 cells. The specific binding of fib-coated-yellow-QDs was further confirmed with alpha5beta1-integrins +ve HeLa and alpha5/beta1-integrins -ve HT29 cells. Cellular uptake studies revealed localization of PTX-fib-coated-yellow-QDs inside MCF-7 cells compared to the normal L929 cells. These results indicated that fib-coated-QDs could be used for targeted imaging and as a suitable "nanocarrier" aiming breast cancer cells.
authors.affiliation: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi 682041, India.
authors.forename: N Sanoj
authors.initials: NS
authors.lastname: Rejinold
authors.forename: Thejus
authors.initials: T
authors.lastname: Baby
authors.forename: Shantikumar V
authors.initials: SV
authors.lastname: Nair
authors.forename: R
authors.initials: R
authors.lastname: Jayakumar
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000737:chemistry; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D019187:Cadmium Compounds / Q000737:chemistry; D045744:Cell Line, Tumor; D004058:Diffusion; D005260:Female; D005340:Fibrinogen / Q000737:chemistry / Q000493:pharmacokinetics*; D006801:Humans; D051379:Mice; D053769:Nanocapsules / Q000737:chemistry / Q000627:therapeutic use*; D017239:Paclitaxel / Q000008:administration & dosage* / Q000737:chemistry; D013691:Tellurium / Q000737:chemistry; D015032:Zinc / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D019187:Cadmium Compounds; D053769:Nanocapsules; D005340:Fibrinogen; D015032:Zinc; D013691:Tellurium; D017239:Paclitaxel; C028337:cadmium telluride