title: Submerged Culture of Phellinus linteus for Mass Production of Polysaccharides.
abstract: In order to increase the mycelial production of Phellinus linteus, which exhibits potent anticancer activity, some ingredients of the medium used to culture P. linteus were investigated. The optimal medium composition for the production of Phellinus linteus was determined to be as follows: fructose, 40 g/l; yeast extract, 20 g/l; K2HPO4, 0.46 g/l; KH2PO4, 1.00 g/l; MgSO4·7H2O, 0.50 g/l; FeCl2·62O, 0.01 g/l; MnCl2·4H2O, 0.036 g/l; ZnCl2, 0.03 g/l; and CuSO4·7H2O, 0.005 g/l. The optimal culture conditions were determined to be as follows: temperature, 28℃; initial pH, 5.5; aeration, 0.6 vvm; and agitation, 100 rpm, respectively. Under optimal composition and conditions, the maximum mycelial biomass achieved in a 5 l jar fermentor was 29.9 g/l.
authors.affiliation: Department of Food Processing and Cooking, Kyungbuk College, Yeongjusi 750-712, Korea.
authors.forename: June Woo
authors.initials: JW
authors.lastname: Lee
authors.forename: Seong Jin
authors.initials: SJ
authors.lastname: Baek
authors.forename: Yong Seok
authors.initials: YS
authors.lastname: Kim
keywords: Cultural condition; Optimal media; Phellinus linteus; Polysaccharide