title: Porphyromonas gingivalis promotes neointimal formation after arterial injury through toll-like receptor 2 signaling.
abstract: We previously demonstrated that Porphyromonas gingivalis infection induces neointimal hyperplasia with an increase in monocyte chemoattractant protein (MCP)-1 after arterial injury in wild-type mice. Toll-like receptor (TLR) 2 is a key receptor for the virulence factors of P. gingivalis. The aim of this study was to assess whether TLR2 plays a role in periodontopathic bacteria-induced neointimal formation after an arterial injury. Wild-type and TLR2-deficient mice were used in this study. The femoral arteries were injured, and P. gingivalis or vehicle was injected subcutaneously once per week. Fourteen days after arterial injury, the murine femoral arteries were obtained for histopathologic and immunohistochemical analyses. The immunoglobulin-G levels of the P. gingivalis-infected groups were significantly increased in comparison with the level in the corresponding noninfected groups in both wild-type and TLR2-deficient mice. TLR2 deficiency negated the P. gingivalis-induced neointimal formation in comparison with the wild-type mice, and reduced the number of positive monocyte chemoattractant protein-1 cells in the neointimal area. These findings demonstrate that P. gingivalis infection can promote neointimal formation after an arterial injury through TLR2 signaling.
authors.affiliation: Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Naho
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Suzuki
authors.forename: Masahito
authors.initials: M
authors.lastname: Ogawa
authors.forename: Norio
authors.initials: N
authors.lastname: Aoyama
authors.forename: Issei
authors.initials: I
authors.lastname: Komuro
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Izumi
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Isobe
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D018932:Chemokine CCL2 / Q000378:metabolism; D004195:Disease Models, Animal; D005263:Femoral Artery / Q000276:immunology / Q000293:injuries / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D006965:Hyperplasia; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D058426:Neointima; D016966:Porphyromonas gingivalis / Q000276:immunology / Q000472:pathogenicity*; D015398:Signal Transduction; D051195:Toll-Like Receptor 2 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D057772:Vascular System Injuries / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000382:microbiology* / Q000473:pathology
chemical_list: D000907:Antibodies, Bacterial; C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D007074:Immunoglobulin G; C493486:Tlr2 protein, mouse; D051195:Toll-Like Receptor 2