title: In vitro protection of adipose tissue-derived mesenchymal stem cells by erythropoietin.
abstract: Mobilization of stem cells and their differentiation into cardiomyocytes are known to have protective effects after myocardial infarction. The integrity of transplanted mesenchymal stem cells for cardiac regeneration is dependent on cell-cell or cell-matrix interaction, which is adversely affected by reactive oxygen species in an ischemic environment. Treatment with erythropoietin was shown to protect human adipose tissue derived mesenchymal stem cells in an ischemic injury in vitro model. The analyses indicated that expression of erythropoietin receptors played a pivotal role in erythropoietin mediated cell survival. In this study, the anti-apoptotic effect of erythropoietin on stem cells was analyzed in apoptosis-induced human mesenchymal stem cells. Apoptosis was induced in cultured adult human adipose tissue derived mesenchymal stem cells by hydrogen peroxide. A group of cultured cells was also treated with recombinant human erythropoietin in a concentration of 50 ng mL(-1). The degree of apoptosis was analyzed by flow-cytometry and immunohistochemical staining for Caspase 3. The average percentages of apoptotic cells were significantly higher in H2O2-induced stem cells than in cells co-cultured with erythropoietin (63.03 ± 4.96% vs 29 ± 3.41%, p<0.01). We conclude that preconditioning with erythropoietin suppresses apoptosis of mesenchymal stem cells and enhances their survival.
authors.affiliation: Department of Cardiology, Faculty of Medicine, Izmir University, Izmir, Turkey.
authors.forename: Ertugrul
authors.initials: E
authors.lastname: Ercan
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey.
authors.forename: Aysel Guven
authors.initials: AG
authors.lastname: Bagla
authors.affiliation: Department of Stem Cells, Center for Stem Cell and Gene Therapies Research and Practice, Kocaeli University, Institute of Health Sciences, Kocaeli, Turkey.
authors.forename: Ayca
authors.initials: A
authors.lastname: Aksoy
authors.affiliation: Department of Stem Cells, Center for Stem Cell and Gene Therapies Research and Practice, Kocaeli University, Institute of Health Sciences, Kocaeli, Turkey.
authors.forename: Gulcin
authors.initials: G
authors.lastname: Gacar
authors.affiliation: Department of Stem Cells, Center for Stem Cell and Gene Therapies Research and Practice, Kocaeli University, Institute of Health Sciences, Kocaeli, Turkey.
authors.forename: Z Seda
authors.initials: ZS
authors.lastname: Unal
authors.affiliation: Department of Cardiovascular Surgery, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey. Electronic address: hfasgun@yahoo.com.
authors.forename: H Fatih
authors.initials: HF
authors.lastname: Asgun
authors.affiliation: Department of Stem Cells, Center for Stem Cell and Gene Therapies Research and Practice, Kocaeli University, Institute of Health Sciences, Kocaeli, Turkey.
authors.forename: Erdal
authors.initials: E
authors.lastname: Karaoz
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D019610:Cytoprotection; D004921:Erythropoietin / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress; D050151:Subcutaneous Fat / Q000166:cytology*; D019098:Telomerase / Q000378:metabolism; D059467:Transcriptome
chemical_list: C554270:EPO protein, human; D016877:Oxidants; D004921:Erythropoietin; D006861:Hydrogen Peroxide; D019098:Telomerase; C505407:CASP3 protein, human; D053148:Caspase 3
keywords: Apoptosis; Erythropoietin; Erythropoietin-receptor; Human adipose tissue; Stem cell