title: [Expression and significance of PD-1 on CD4(+); and CD8(+); T cells from the peripheral blood of patients with systemic lupus erythematosus].
abstract: OBJECTIVE
  To detect the expression of programmed death-1 (PD-1) on CD4(+); and CD8(+); T cells from the peripheral blood of patients with systemic lupus erythematosus (SLE) and analyze its clinical implications.
  
  
  METHODS
  The expression of PD-1 on the CD4(+); and CD8(+); T cells were examined in 51 SLE patients and 38 healthy controls (HCs) by flow cytometry. The proportions of PD-1 positive CD4(+); and CD8(+); T cells were compared not only between inactive or active SLE patients and HCs, but also between patients with and without lupus nephritis. The correlations of PD-1 expression with clinical manifestations and laboratory findings were analyzed.
  
  
  RESULTS
  The proportions of CD4(+); PD-1(+); T cells significantly increased in active SLE patients as compared with HCs and inactive SLE ones (all P<0.05). The proportions of CD8(+); PD-1(+); T cells were significantly higher in active and inactive SLE patients than those in HCs (all P<0.05). The numbers of CD4(+); PD-1(+); and CD8(+); PD-1(+); T cells in SLE patients with nephritis were significantly higher than those in patients without nephritis (P<0.01). In SLE patients, the numbers of CD4(+);PD-1(+); and CD8(+);PD-1(+); T cells were significantly higher in ones with positive anti-dsDNA, anti-Sm, and anti-nucleosome antibodies than in corresponding negative ones; the proportions of CD4(+); PD-1(+); and CD8(+); PD-1(+); T cells were positively correlated with SLEDAI score and the amount of proteinuria, but were negatively correlated with complement C3.
  
  
  CONCLUSIONS
  The aberrations of CD4(+); PD-1(+); and CD8(+); PD-1(+); T cells were observed in patients with SLE. Increased numbers of CD4(+); PD-1(+); and CD8(+); PD-1(+); T cells were correlated with increased disease activity and the production of anti-self antibodies.
authors.affiliation: Department of Histology and Embryology, Bengbu Medical College, Bengbu, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Changhao
authors.initials: C
authors.lastname: Xie
authors.forename: Meiqun
authors.initials: M
authors.lastname: Sun
authors.forename: Jixia
authors.initials: J
authors.lastname: Chai
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Zhou
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Tang
authors.forename: Boqing
authors.initials: B
authors.lastname: Li
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry*; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry*; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008297:Male; D061026:Programmed Cell Death 1 Receptor / Q000097:blood* / Q000502:physiology
chemical_list: C105992:PDCD1 protein, human; D061026:Programmed Cell Death 1 Receptor; D007371:Interferon-gamma