title: Psychrotrophic Pseudomonas mandelii CBS-1 produces high levels of poly-β-hydroxybutyrate.
abstract: A novel facultative psychrotroph (strain CBS-1), which accumulates poly-β-hydroxybutyrate (PHB), was isolated from soil samples taken from Changbai Mountain, China. Phylogenetic analysis based on 16S rRNA sequence data and Biolog analysis identified strain CBS-1 as Pseudomonas mandelii. Transmission electron micrographs revealed abundant electron-transparent intracellular granules. (1)H-nuclear magnetic resonance analysis revealed that the granules were composed of PHB. P. mandelii CBS-1 grew optimally at 20°C. When cultured aerobically for 48 h with sucrose as the sole carbon source, strain CBS-1 yielded a maximum cell density of 29.3 g/L cell dry weight and synthesized 22.3 g/L of PHB. The ability of strain CBS-1 to grow at a low temperature and rapidly synthesize high levels of PHB may reduce the costs of industrial PHB production.
authors.affiliation: Colloge of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211800 People's Republic of China.
authors.forename: Rongpeng
authors.initials: R
authors.lastname: Li
authors.forename: Yuji
authors.initials: Y
authors.lastname: Jiang
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Wang
authors.forename: Jingjing
authors.initials: J
authors.lastname: Yang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zi
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.forename: Haofeng
authors.initials: H
authors.lastname: Gao
authors.forename: Nan
authors.initials: N
authors.lastname: Hu
keywords: 1H- nuclear magnetic resonance; Facultative psychrotrophs; Fermentation; PHB; Pseudomonas mandelii CBS-1