title: Polysaccharide structure of tetrasporic red seaweed Tichocarpus crinitus.
abstract: Sulfated polysaccharide isolated from tetrasporic plants of Tichocarpus crinitus was investigated. The polysaccharide was isolated by two methods: with water extraction at 80 °C (HT) and with a mild alkaline extraction (AE). The extracted polysaccharides were presented by non-gelling ones only, while galactose and 3,6-AG were the main monosaccharides, at the same time amount of 3,6-AG in AE polysaccharides was the similar to that of HT. According to methods of spectroscopy and mass spectrometry, the polysaccharide from tetrasporic T. crinitus contains main blocks of 1,3-linked β-D-galactopyranosyl-2,4-disulfates and 1,4-linked 3,6-anhydro-α-D-galactopyranosyl while 6-sulfated 4-linked galactopyranosyl resudies are randomly distributed along the polysaccharide chain. The alkaline treatment of HT polysaccharide results in obtaining polysaccharide with regular structure that composed of alternating 1,3-linked β-D-galactopyranosyl-2,4-disulfates and 1,4-linked 3,6-anhydro-α-D-galactopyranosyl residues. Native polysaccharide (HT) possessed both high anticoagulant and antiplatelet activity measured by fibrin clotting and platelet aggregation induced by collagen. This activity could be connected with peculiar chemical structure of HT polysaccharide which has high sulfation degree and contains also 3,6-anhydrogalactose in the polymer chain.
authors.affiliation: G.B. Elyakov Paciﬁc Institute of Bioorganic Chemistry, Far-East Branch of the Russian Academy of Sciences, Prospect 100-let Vladivostoku, 159, Vladivostok 690022, Russia.
authors.forename: A O
authors.initials: AO
authors.lastname: Byankina Barabanova
authors.forename: E V
authors.initials: EV
authors.lastname: Sokolova
authors.forename: S D
authors.initials: SD
authors.lastname: Anastyuk
authors.forename: V V
authors.initials: VV
authors.lastname: Isakov
authors.forename: V P
authors.initials: VP
authors.lastname: Glazunov
authors.forename: A V
authors.initials: AV
authors.lastname: Volod'ko
authors.forename: I M
authors.initials: IM
authors.lastname: Yakovleva
authors.forename: T F
authors.initials: TF
authors.lastname: Solov'eva
authors.forename: I M
authors.initials: IM
authors.lastname: Yermak
mesh_terms: D006801:Humans; D008970:Molecular Weight; D010974:Platelet Aggregation / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D000461:Rhodophyta / Q000737:chemistry*; D012624:Seaweed / Q000737:chemistry*; D013918:Thrombin Time
chemical_list: D011134:Polysaccharides
keywords: Carrageenan; Life history stage of alga; Structure; Tetrasporophytes