title: Spatiotemporal expression of Wnt signaling pathway components during bovine placental development.
abstract: It is well established that trophoblasts play a crucial role in pregnancy establishment and maintenance through production of various biological substances. In this regard, Wnt signaling is an important regulator of embryo implantation and placentation in various species. However, the role of the Wnt signaling pathway during bovine placental development has remained largely unknown. Employing multiple approaches, we herein found that Wnt2 mRNA was more abundant in cotyledon tissues compared with caruncle tissues, whereas Wnt5b mRNA was more abundant in caruncle tissues compared with cotyledon tissues. Moreover, the Wnt receptor Fzd4 was detected in caruncle epithelial cells and binucleate trophoblasts, but not in uninucleate trophoblasts. In addition, β-catenin, an integral cell-cell adhesion adaptor protein as well as transcriptional co-regulator of Wnt canonical pathway, was spatiotemporally expressed in bovine trophoblasts, with high levels of cellular accumulation and nuclear translocation, particularly in binucleate trophoblasts. Lymphoid enhancer factor-1 mRNA was more abundant in caruncle tissues compared with cotyledon tissues, which was well correlated with the expression profile of Dickkopf-1, a secreted antagonist of the canonical Wnt signaling pathway. These results provided new evidence that precisely regulated canonical Wnt activation may have a very important physiological role during fetal-maternal recognition and pregnancy maintenance in cattle.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, PR China; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing High-tech Industrial Development Zone, Daqing, PR China. Electronic address: lwg1712@hotmail.com.
authors.forename: Wengeng
authors.initials: W
authors.lastname: Lu
authors.forename: Zhaowei
authors.initials: Z
authors.lastname: Tu
authors.forename: Shumin
authors.initials: S
authors.lastname: Wang
authors.forename: Jinhua
authors.initials: J
authors.lastname: Lu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Weixiang
authors.initials: W
authors.lastname: Wang
authors.forename: Bingyan
authors.initials: B
authors.lastname: Wang
authors.forename: Haibin
authors.initials: H
authors.lastname: Wang
authors.forename: Hemin
authors.initials: H
authors.lastname: Ni
authors.forename: Yong
authors.initials: Y
authors.lastname: Guo
mesh_terms: D000818:Animals; D002417:Cattle / Q000254:growth & development / Q000378:metabolism*; D005260:Female; D051157:Frizzled Receptors / Q000032:analysis / Q000378:metabolism; D017403:In Situ Hybridization; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis / Q000378:metabolism; D010920:Placenta / Q000166:cytology / Q000378:metabolism*; D010929:Placentation; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D051153:Wnt Proteins / Q000032:analysis / Q000378:metabolism; D060449:Wnt Signaling Pathway; D051154:Wnt2 Protein / Q000378:metabolism; D051176:beta Catenin / Q000032:analysis / Q000378:metabolism
chemical_list: D051157:Frizzled Receptors; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D051153:Wnt Proteins; D051154:Wnt2 Protein; D051176:beta Catenin
keywords: Bovine placenta; Trophoblasts; Wnt