title: Ethanol extract of the Prunus mume fruits stimulates glucose uptake by regulating PPAR-γ in C2C12 myotubes and ameliorates glucose intolerance and fat accumulation in mice fed a high-fat diet.
abstract: In this study, we performed in vitro and in vivo studies to examine whether a 70% ethanol extract of Prunus mume fruits (EMS) exhibits anti-diabetic effects. Treatment with EMS increased glucose uptake in C2C12 myotubes, and also increased PPAR-γ activity or PPAR-γ mRNA expression. To confirm these in vitro results, we next conducted an animal experiment. A high-fat diet significantly increased the body weight, fat accumulation, and glucose levels in mice. Under the same conditions, 5% EMS attenuated the high-fat diet-induced increase in body weight and fat accumulation and improved the impaired fasting glucose level and glucose tolerance. High performance liquid chromatography analysis demonstrated that EMS contained chlorogenic acid, caffeic acid, rutin, luteolin-7-glucoside, naringin, apigenin-7-glucoside, and hesperidin. Taken together, these findings suggest that EMS exerts an anti-diabetic effect both in vitro and in vivo, which is mediated, at least in part, by the activation of PPAR-γ.
authors.affiliation: Korea Food Research Institute, Republic of Korea.
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Shin
authors.forename: Haeng Jeon
authors.initials: HJ
authors.lastname: Hur
authors.forename: Mi Jeong
authors.initials: MJ
authors.lastname: Sung
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Park
authors.forename: Hye Jeong
authors.initials: HJ
authors.lastname: Yang
authors.forename: Myung Sunny
authors.initials: MS
authors.lastname: Kim
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Kwon
authors.forename: Jin-Taek
authors.initials: JT
authors.lastname: Hwang
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D059305:Diet, High-Fat / Q000009:adverse effects; D005223:Fats / Q000378:metabolism*; D005638:Fruit / Q000737:chemistry; D005947:Glucose / Q000378:metabolism*; D018149:Glucose Intolerance / Q000188:drug therapy* / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D009765:Obesity / Q000188:drug therapy* / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics* / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D027861:Prunus / Q000737:chemistry*
chemical_list: D005223:Fats; D047495:PPAR gamma; D010936:Plant Extracts; D005947:Glucose
keywords: Anti-diabetes; High fat diet; Myotube; PPAR-γ; Prunus mume fruit