title: Variations in lethal and sublethal effects of cypermethrin among aquatic stages and species of anuran amphibians.
abstract: Despite the use of model species to predict the effects of chemicals in the environment, unpredicted variation in levels of risk to organisms from xenobiotics can be observed. Physiological and morphological differences between species and life stages may lead to differences in sensitivity, while seasonal and spatial variation in pesticide concentrations may affect the level of risk faced by organisms in the environment. Because anurans breed in aquatic habitats subject to contamination by runoff and spraying, they are particularly vulnerable to pesticides. In the present study, embryos, newly hatched larvae, and larvae with limb buds of 3 anuran amphibian species--Pseudacris regilla, Rana cascadae, and Rana aurora--were exposed for 48 h to either 0.5 µg/L or 5.0 µg/L cypermethrin under laboratory conditions. The authors monitored hatching success, larval survival, and measured growth. Additionally, they assayed avoidance behavior 2 wk after exposure or 2 wk after hatching for individuals exposed as embryos. Hatching and survival were not affected in animals of any species exposed as embryos. After exposure as embryos and as newly hatched larvae, however, P. regilla displayed behavioral abnormalities in response to prodding. Cypermethrin increased mortality in P. regilla exposed in both larval stages. Cypermethrin also increased mortality in larval R. cascadae when exposed at the early stage. These results indicate variation in sensitivity to environmentally relevant concentrations of cypermethrin among anuran species and life stages.
authors.affiliation: Environmental Sciences Graduate Program, Oregon State University, Corvallis, Oregon, USA.
authors.forename: Lindsay M
authors.initials: LM
authors.lastname: Biga
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Blaustein
mesh_terms: D000818:Animals; D001001:Anura / Q000196:embryology* / Q000254:growth & development*; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000502:physiology; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D010575:Pesticides / Q000633:toxicity*; D011722:Pyrethrins / Q000633:toxicity*; D011898:Ranidae / Q000196:embryology / Q000254:growth & development; D013045:Species Specificity; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D010575:Pesticides; D011722:Pyrethrins; D014874:Water Pollutants, Chemical; C017160:cypermethrin
keywords: Amphibian; Embryo; Larva; Pyrethroid; Toxic effect