title: Synthesis, DNA/protein binding and in vitro cytotoxic studies of new palladium metallothiosemicarbazones.
abstract: A series of four new thiosemicarbazone complexes of palladium have been synthesized, characterized and evaluated for their DNA/protein binding with CT-DNA and BSA, respectively. The new complexes bound to CT-DNA by intercalation mode and in protein binding studies, the complexes bound to BSA binding mechanism was found as static quenching. Among them the complex 4 had a strong binding affinity with BSA. In addition, in vitro cytotoxic studies were carried out on lung cancer (A549) and liver cancer (HepG2) cell lines and found that the complexes exhibited better activity than their parent thiosemicarbazone analogues. The complex 3 exhibited better activity than other complexes and this fact supported by the increased accumulation of the complexes in to the cancer cells which are evident from inter cellular uptake studies.
authors.affiliation: Department of Chemistry, Bharathiar University, Coimbatore 641 046, India. Electronic address: rpnchemist@gmail.com.
authors.forename: R
authors.initials: R
authors.lastname: Prabhakaran
authors.forename: P
authors.initials: P
authors.lastname: Kalaivani
authors.forename: P
authors.initials: P
authors.lastname: Poornima
authors.forename: F
authors.initials: F
authors.lastname: Dallemer
authors.forename: R
authors.initials: R
authors.lastname: Huang
authors.forename: V
authors.initials: V
authors.lastname: Vijaya Padma
authors.forename: K
authors.initials: K
authors.lastname: Natarajan
mesh_terms: D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000378:metabolism / Q000633:toxicity; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D007700:Kinetics; D008968:Molecular Conformation; D009573:Nitrites / Q000378:metabolism; D010165:Palladium / Q000737:chemistry*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D013882:Thiosemicarbazones / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D009573:Nitrites; D013882:Thiosemicarbazones; D012710:Serum Albumin, Bovine; D010165:Palladium; D004247:DNA
keywords: BSA binding; CT-DNA binding; Cellular uptake; Cytotoxicity; Palladium(II) complexes; Thiosemicarbazones