title: Labeling individual neurons in the brains of live Xenopus tadpoles by electroporation of dyes or DNA.
abstract: This protocol describes the targeted introduction of fluorophore in the form of a dye or genetic material into single cells. This method has the advantage of producing true single-cell chimeric animals in which to study the effects of overexpression or knockdown of a gene in an otherwise entirely wild-type background.
authors.forename: Edward S
authors.initials: ES
authors.lastname: Ruthazer
authors.forename: Anne
authors.initials: A
authors.lastname: Schohl
authors.forename: Neil
authors.initials: N
authors.lastname: Schwartz
authors.forename: Aydin
authors.initials: A
authors.lastname: Tavakoli
authors.forename: Marc
authors.initials: M
authors.lastname: Tremblay
authors.forename: Hollis T
authors.initials: HT
authors.lastname: Cline
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology*; D003584:Cytological Techniques / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism; D007814:Larva / Q000166:cytology; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000502:physiology*; D013194:Staining and Labeling / Q000379:methods*; D014981:Xenopus / Q000196:embryology*
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins