title: Think modular: a simple apoferritin-based platform for the multifaceted detection of pancreatic cancer.
abstract: The generation of nanosized probes often requires time-intensive and application-specific optimization processes that involve conjugating a nanoconstruct to a targeting moiety. Herein, we genetically modify apoferritin and generate a universal interface system composed of protein G and 6×His-tag. The resulting construct, conferred with modularity and high targeting efficiency, is applied toward two distinct applications in the detection of a pancreatic cancer biomarker and used to demonstrate its potential in the facile exchange of nanoprobe components.
authors.affiliation: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology (KIS T), 5 Hwarangno 14-gil, Seongbuk-gu, Seoul 136-791, Republic of Korea.
authors.forename: Mintai P
authors.initials: MP
authors.lastname: Hwang
authors.forename: Jong-Wook
authors.initials: JW
authors.lastname: Lee
authors.forename: Kyung Eun
authors.initials: KE
authors.lastname: Lee
authors.forename: Kwan Hyi
authors.initials: KH
authors.lastname: Lee
mesh_terms: D001052:Apoferritins / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D062506:Claudin-4 / Q000378:metabolism*; D006801:Humans; D008297:Male; D010190:Pancreatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D045663:Quantum Dots; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D062506:Claudin-4; D001052:Apoferritins