title: The use of an engineered single chain variable fragment in a localized surface plasmon resonance method for analysis of the C-reactive protein.
abstract: A gold nanorod (GNR) based LSPR sensor has been developed for label-free detection of C-reactive protein (CRP). The sensor utilizes a single chain variable fragment (scFv) as a receptor to bind CRP. The results of this effort show that CRP in human serum can be quantitatively detected at lower than 1 ng mL(-1).
authors.affiliation: Department of Chemistry, School of Physics and Chemistry and Advanced Photonics Research Institute, Gwangju Institute of Science and Technology (GIST), Korea. mkim@gist.ac.kr.
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Byun
authors.forename: Yong-Beom
authors.initials: YB
authors.lastname: Shin
authors.forename: Taihua
authors.initials: T
authors.lastname: Li
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Park
authors.forename: Dong-Myung
authors.initials: DM
authors.lastname: Kim
authors.forename: Dong-Hwan
authors.initials: DH
authors.lastname: Choi
authors.forename: Min-Gon
authors.initials: MG
authors.lastname: Kim
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D002097:C-Reactive Protein / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D043942:Nanotubes / Q000737:chemistry; D057127:Single-Chain Antibodies / Q000737:chemistry* / Q000378:metabolism; D020349:Surface Plasmon Resonance
chemical_list: D057127:Single-Chain Antibodies; D006046:Gold; D002097:C-Reactive Protein