title: Monitoring infection: from blood culture to polymerase chain reaction (PCR).
abstract: In patients with sepsis, diagnosis of blood stream infection (BSI) is a key concern to the therapist. Direct verification of pathogens in the blood stream executed by blood cultures (BC) still is regarded as the gold standard up to date. The quickest possible initiation of an appropriate antimicrobial therapy is a cornerstone of an effective therapy. Moreover, in this view BC can also serve to identify antimicrobial agents to target the pathogen. However, when employing BC the time needed until microbiological results are available ranges from 24 up to 72 h. Moreover, infections caused by multiple pathogens often remain undetected and concurrent antibiotic therapy may lower the overall sensitivity. Alternative pathogen characterization can be performed by polymerase chain reaction (PCR) based amplification methods. Results using PCR can be obtained within 6-8 h. Therefore, the time delay until an appropriate therapy can be reduced enormously. Moreover, these methods have the potential to enhance the sensitivity in the diagnosis of blood stream infections. Therefore, PCR based methods might be a valuable adjunct to present procedures of diagnosing bacteraemia.
authors.affiliation: University Department of Anaesthesiology and Pain Medicine, Inselspital, Freiburgstrasse, CH-3010 Bern, Switzerland. Malte.Book@dkf.unibe.ch
authors.forename: Malte
authors.initials: M
authors.lastname: Book
authors.forename: Lutz Eric
authors.initials: LE
authors.lastname: Lehmann
authors.forename: XiangHong
authors.initials: X
authors.lastname: Zhang
authors.forename: Frank
authors.initials: F
authors.lastname: Stüber
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use; D016470:Bacteremia / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D001419:Bacteria / Q000302:isolation & purification; D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents
keywords: PCR; nucleic acid based pathogen detection; real time PCR; sepsis