title: DNA terminal mismatch-induced stabilization of polymer micelles from RAFT-generated poly(N-isopropylacrylamide)-DNA block copolymers.
abstract: Temperature-responsive diblock copolymers made of poly(N-isopropylacrylamide) (PNIPAAm) generated by reversible addition-fragmentation chain transfer (RAFT) polymerization and a single-stranded DNA (ssDNA) self-assembled into polymer micelles. The micelles consisted of the PNIPAAm core surrounded by the ssDNA corona with a hydrodynamic diameter up to 300 nm in an aqueous medium above the lower critical solution temperature. In a medium of high ionic strength, the formation of the fully matched duplex with the complementary ssDNA on the surface of the polymer micelles induced rapid and spontaneous aggregation. By contrast, the micelles remained dispersed under the identical conditions when single-base-substituted ssDNA was added to form the corresponding terminal-mismatched duplex on the micellar surface. This highly sequence-selective process took place irrespective of the size of the PNIPAAm core.
authors.affiliation: Bioengineering Laboratory, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198 (Japan), Fax: (+81) 4-8462-4658; Current address: Department of Chemistry, Faculty of Science, Tokyo University of Science , 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601 (Japan).
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Isoda
authors.forename: Naoki
authors.initials: N
authors.lastname: Kanayama
authors.forename: Masahiro
authors.initials: M
authors.lastname: Fujita
authors.forename: Tohru
authors.initials: T
authors.lastname: Takarada
authors.forename: Mizuo
authors.initials: M
authors.lastname: Maeda
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D020137:Base Pair Mismatch; D004247:DNA / Q000737:chemistry*; D008823:Micelles; D015394:Molecular Structure; D058105:Polymerization
chemical_list: D000180:Acrylic Resins; D008823:Micelles; C052970:poly-N-isopropylacrylamide; D004247:DNA
keywords: DNA; block copolymers; colloids; micelles; thermoresponsive polymers