title: Expression, crystallization and preliminary X-ray crystallographic analysis of alanine racemase from Acinetobacter baumannii OXA-23.
abstract: Acinetobacter baumannii has received much attention owing to its exceptional ability to develop resistance to currently available antibiotics. Alanine racemase (ALR) catalyzes the racemization of L-alanine to D-alanine with pyridoxal 5'-phosphate (PLP) as a cofactor. The D-alanine product is an essential component of the bacterial cell wall and ALR is a potential target for the development of novel antibacterial drugs. The alr gene from A. baumannii was cloned and the protein (AbALR) was expressed, purified and crystallized. The AbALR crystal diffracted to 2.3 Å resolution and belonged to the primitive orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 55.1, b = 85.0, c = 167.7 Å. Two protomers were present in the asymmetric unit, with a corresponding V(M) value of 2.3 Å(3) Da(-1) and a solvent content of 47.5%.
authors.affiliation: Department of Advanced Technology Fusion, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Dinh-Duc
authors.initials: DD
authors.lastname: Nguyen
authors.forename: Ho-Phuong-Thuy
authors.initials: HP
authors.lastname: Ngo
authors.forename: Myoung-ki
authors.initials: MK
authors.lastname: Hong
authors.forename: Tan-Viet
authors.initials: TV
authors.lastname: Pham
authors.forename: Jung Hun
authors.initials: JH
authors.lastname: Lee
authors.forename: Jae Jin
authors.initials: JJ
authors.lastname: Lee
authors.forename: Dae Beom
authors.initials: DB
authors.lastname: Kwon
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Lin-Woo
authors.initials: LW
authors.lastname: Kang
mesh_terms: D040981:Acinetobacter baumannii / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D000411:Alanine Racemase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D000411:Alanine Racemase
keywords: Acinetobacter baumannii; PLP; alanine racemase