title: Simultaneous determination of phenolic acids by UPLC-MS/MS in rat plasma and its application in pharmacokinetic study after oral administration of Flos Lonicerae preparations.
abstract: The current study aims to investigate the pharmacokinetic study of five phenolic acids (neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 3,5-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid) following oral administration of Flos Lonicerae preparations in rats. A rapid and sensitive ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed to simultaneously determine the five phenolic acids in rat plasma. After mixing with the internal standard (IS) tinidazole, plasma samples were pretreated by liquid-liquid extraction with ethyl acetate/n-hexane (9:1, v/v). The separation was performed on an Acquity UPLC BEH C18 column (100mm×2.1mm, 1.7μm) at a flow rate of 0.4mlmin(-1), and acetonitrile/methanol (4:1, v/v)-0.4% formic acid was used as mobile phase. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) via electrospray ionization (ESI) source with positive ionization mode. All calibration curves had good linearity (r>0.991) over the concentration ranges of 0.74-378ngml(-1) for neochlorogenic acid, 0.50-1030ngml(-1) for chlorogenic acid, 1.9-250ngml(-1) for cryptochlorogenic acid, 0.74-380ngml(-1) for 3,5-dicaffeoylquinic acid, and 5.1-328ngml(-1) for 3,4-dicaffeoylquinic acid. The intra-and inter-day precision were within 15% and the accuracy ranged from 86.2% to 114.1%.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, PR China; Jiangsu Engineering Research Center for Efficient Delivery System of TCM, PR China; Nanjing Engineering Research Center for Industrialization of Chinese Medicine Pellets, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Shijia
authors.initials: S
authors.lastname: Liu
authors.forename: Wenzheng
authors.initials: W
authors.lastname: Ju
authors.forename: Jinjun
authors.initials: J
authors.lastname: Shan
authors.forename: Minxin
authors.initials: M
authors.lastname: Meng
authors.forename: Baochang
authors.initials: B
authors.lastname: Cai
authors.forename: Liuqing
authors.initials: L
authors.lastname: Di
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D062385:Hydroxybenzoates / Q000097:blood* / Q000493:pharmacokinetics; D031239:Lonicera; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D010936:Plant Extracts / Q000008:administration & dosage* / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D062385:Hydroxybenzoates; D010936:Plant Extracts; C588038:lonicerae flos; C017616:phenolic acid
keywords: Flos Lonicerae; Oral delivery; Pharmacokinetics; Phenolic acids; UPLC–MS/MS