title: Expression of early growth response-1 in human gastric cancer and its relationship with tumor cell behaviors and prognosis.
abstract: The early growth response-1 (Egr-1) is crucial in many cell regulatory processes related to the progression of human cancers. Its overexpression has been demonstrated in variable human cancers and may have prognostic significance. The aims of this current study were to evaluate whether Egr-1 affects invasive and oncogenic phenotypes of human gastric cancer cells, and to examine the relationships between its expression and various clinicopathological parameters, including survival in human gastric cancer patients. We investigated the biologic role of Egr-1 in tumor cell behavior by using a small interfering RNA in human gastric cancer cell lines, AGS and TMK1. The expression of Egr-1 by reverse transcription-polymerase chain reaction (RT-PCR), Western blotting and immunohistochemistry was investigated in human gastric cancer tissues. The knockdown of Egr-1 suppressed tumor cell migration and invasion in AGS and TMK1 cells. Egr-1 expression was significantly increased in human gastric cancer and metastatic lymph node tissues compared to the normal gastric mucosa and non-metastatic lymph node tissues. Positive expression of Egr-1 was significantly associated with tumor size, depth of invasion, lymph node metastasis, tumor stage and poor survival. These results indicate that Egr-1 is associated with human gastric cancer progression through the alteration of tumor cell behavior, such as migration and invasion. Egr-1 expression may help in predicting the clinical outcomes of human gastric cancer patients.
authors.affiliation: Department of Internal Medicine, Chonnam National University Medical School, Gwangju 501-757, Republic of Korea.
authors.forename: Eun
authors.initials: E
authors.lastname: Myung
authors.forename: Young-Lan
authors.initials: YL
authors.lastname: Park
authors.forename: Nuri
authors.initials: N
authors.lastname: Kim
authors.forename: Cho-Yun
authors.initials: CY
authors.lastname: Chung
authors.forename: Hyun-Bum
authors.initials: HB
authors.lastname: Park
authors.forename: Hyung-Chul
authors.initials: HC
authors.lastname: Park
authors.forename: Dae-Sung
authors.initials: DS
authors.lastname: Myung
authors.forename: Jong-Sun
authors.initials: JS
authors.lastname: Kim
authors.forename: Sung-Bum
authors.initials: SB
authors.lastname: Cho
authors.forename: Wan-Sik
authors.initials: WS
authors.lastname: Lee
authors.forename: Young-Eun
authors.initials: YE
authors.lastname: Joo
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D051766:Early Growth Response Protein 1 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D016016:Proportional Hazards Models; D034622:RNA Interference; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D013997:Time Factors; D014162:Transfection; D016896:Treatment Outcome; D047368:Tumor Burden
chemical_list: C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1
keywords: Egr-1; Prognosis; Stomach neoplasm; Tumor behaviors