title: Isocitrate dehydrogenase 1 (IDH1) mutation-specific microRNA signature predicts favorable prognosis in glioblastoma patients with IDH1 wild type.
abstract: BACKGROUND
  To date, no prognostic microRNAs (miRNAs) for isocitrate dehydrogenase 1 (IDH1) wild-type glioblastoma multiformes (GBM) have been reported. The aim of the present study was to identify a miRNA signature of prognostic value for IDH1 wild-type GBM patients using miRNA expression dataset from the The Cancer Genome Atlas (TCGA).
  
  
  METHODS
  Differential expression profiling analysis of miRNAs was performed on samples from 187 GBM patients, comprising 17 mutant-type IDH1 and 170 wild-type IDH1 samples.
  
  
  RESULTS
  A 23-micoRNA signature which was specific to the IDH1 mutation was revealed. Survival data was available for 140 of the GBM patients with wild-type IDH1. Using these data, the samples were characterized as high-risk or low-risk group according to the ranked protective scores for each of the 23 miRNAs in the 23-miRNA signature. Then, the 23 IDH1 mutation-specific miRNAs were classified as risky group and protective group miRNAs based on the significance analysis of microarrays d-score (SAM d-value) (positive value or negative value). The risky group miRNAs were found to be expressed more in the high-risk samples while the protective group miRNAs were expressed more in the low-risk samples. Patients with high protective scores had longer survival times than those with low protective scores.
  
  
  CONCLUSIONS
  These findings show that IDH1 mutation-specific miRNA signature is a marker for favorable prognosis in primary GBM patients with the IDH1 wild type.
authors.affiliation: Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No, 6 Tiantan Xili, Dongcheng District, Beijing 100050, China. Gujun_2013@163.com.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhaoshi
authors.initials: Z
authors.lastname: Bao
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
authors.forename: Gan
authors.initials: G
authors.lastname: You
authors.forename: Yinyan
authors.initials: Y
authors.lastname: Wang
authors.forename: Xuejun
authors.initials: X
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D001932:Brain Neoplasms / Q000201:enzymology* / Q000235:genetics*; D005909:Glioblastoma / Q000201:enzymology* / Q000235:genetics*; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D009154:Mutation; D011379:Prognosis
chemical_list: D035683:MicroRNAs; D007521:Isocitrate Dehydrogenase; C543588:IDH1 protein, human