title: Optimizing E. coli-based membrane protein production using Lemo21(DE3) and GFP-fusions.
abstract: Optimizing the conditions for the overexpression of membrane proteins in E. coli and their subsequent purification is usually a laborious and time-consuming process. Combining the Lemo21(DE3) strain, which conveniently allows to identify the optimal expression intensity of a membrane protein using only one strain, and membrane proteins C-terminally fused to Green Fluorescent Protein (GFP) greatly facilitates the production of high-quality membrane protein material for functional and structural studies.
authors.affiliation: Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Hjelm
authors.forename: Susan
authors.initials: S
authors.lastname: Schlegel
authors.forename: Thomas
authors.initials: T
authors.lastname: Baumgarten
authors.forename: Mirjam
authors.initials: M
authors.lastname: Klepsch
authors.forename: David
authors.initials: D
authors.lastname: Wickström
authors.forename: David
authors.initials: D
authors.lastname: Drew
authors.forename: Jan-Willem
authors.initials: JW
authors.lastname: de Gier
mesh_terms: D019149:Bioreactors; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D014169:Transformation, Bacterial
chemical_list: D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins