title: The effect of carbon dioxide pneumoperitoneum on the healing colonic anastomosis in rats.
abstract: OBJECTIVE
  To investigate the effects of pneumoperitoneum on colonic anastomosis healing.
  
  
  METHODS
  Colonic anastomosis was performed in 120 rats divided into four groups: Group I - pneumoperitoneum before laparotomy, Group II - pneumoperitoneum after laparorrhaphy, Group III - pneumoperitoneum before laparotomy and after laparorrhaphy, Group IV - no pneumoperitoneum (control group). Pneumoperitoneum pressure was 5 mmHg. Animals were killed on the 3rd, 7th and 14th postoperative day. Histopathological features, anastomosis breaking strength, collagen histomorphometry and hydroxyproline concentration were assessed.
  
  
  RESULTS
  Breaking strength between groups: (day 3, p=0.165; day 7, p=0.219; day 14, p=0.539). Histopathology revealed that group II had, on day 7, less infiltration of mononuclear cells (p=0.006), greater infiltration of polymorphonuclear cells (p=0.001) and greater necrosis (p=0.001); and on day 14, less fibrosis. Histomorphometry revealed a decrease in collagen in groups I and III (p<0.001) on day 7 and an increase in groups I and II on day 14 (p<0.001). Hydroxyproline concentration was similar for groups on days 3 (p=0.152), 7 (p=0.913) or 14 (p=0.981).
  
  
  CONCLUSIONS
  Carbon dioxide does not impair the healing of colonic anastomosis in rats.
authors.affiliation: Postgraduate Program in Medical Sciences, School of Medicine,, University of Brasilia (UnB), Brasilia-DF, Brazil.
authors.forename: Leonardo de Castro
authors.initials: Lde C
authors.lastname: Durães
authors.forename: Igor Eduardo Caetano de
authors.initials: IE
authors.lastname: Farias
authors.forename: Pedro Henrique Alves de
authors.initials: PH
authors.lastname: Morais
authors.forename: Paulo Gonçalves de
authors.initials: PG
authors.lastname: Oliveira
authors.forename: Florêncio Figueiredo
authors.initials: FF
authors.lastname: Cavalcanti Neto
authors.forename: Ruy Souza
authors.initials: RS
authors.lastname: Lino Junior
authors.forename: Omar
authors.initials: O
authors.lastname: Feres
authors.forename: João Batista de
authors.initials: JB
authors.lastname: Sousa
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D002245:Carbon Dioxide / Q000627:therapeutic use*; D003094:Collagen / Q000032:analysis; D003106:Colon / Q000473:pathology / Q000601:surgery*; D007813:Laparotomy; D008297:Male; D009336:Necrosis; D011028:Pneumoperitoneum, Artificial / Q000379:methods*; D011184:Postoperative Period; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013718:Tensile Strength; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D002245:Carbon Dioxide; D003094:Collagen