title: Pharmacology of cannabinoid receptor agonists and a cyclooxygenase-2 inhibitor in rat bone tumor pain.
abstract: We evaluated the pharmacology of spinal selective cannabinoid (CB) receptor agonists and a cyclooxygenase-2 (COX-2) inhibitor on bone tumor pain. MRMT-1 tumor cells were injected into the tibia of female Sprague-Dawley rats. MRMT-1 tumor cells produced a bone tumor confirmed by radiologic and histological findings. Intrathecal CB1 (ACEA) and CB2 receptor (AM 1241) agonists and a COX-2 inhibitor (DuP 697) dose-dependently increased the withdrawal threshold. The calculated ED50 (nmol/l) values for ACEA, AM 1241 and DuP 697 were 0.007, 2.3 and 76.1, respectively. Reverse transcriptase polymerase chain reaction and Western blot showed that COX-2 mRNA and protein, but not CB1 or CB2 receptor, were increased in the spinal cords of rats with bone tumors. Spinal CB1 receptor and CB2 receptor agonists and COX-2 inhibitor may be useful in the management of bone tumor pain. Furthermore, CB2 receptor agonist may be more potent than CB1 receptor agonist and COX-2 inhibitor.
authors.affiliation: Department of Anesthesiology and Pain Medicine, Chonnam National University, Medical School, Gwangju, Korea.
authors.forename: Jin Hua
authors.initials: JH
authors.lastname: Cui
authors.forename: Jin
authors.initials: J
authors.lastname: Ju
authors.forename: Myung Ha
authors.initials: MH
authors.lastname: Yoon
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology; D001859:Bone Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D063386:Cannabinoid Receptor Agonists / Q000494:pharmacology*; D002186:Cannabinoids / Q000494:pharmacology; D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology*; D005260:Female; D006930:Hyperalgesia / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D010146:Pain / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D043884:Receptor, Cannabinoid, CB1 / Q000235:genetics / Q000378:metabolism; D043885:Receptor, Cannabinoid, CB2 / Q000235:genetics / Q000378:metabolism; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism; D013876:Thiophenes / Q000494:pharmacology; D013977:Tibia / Q000187:drug effects / Q000473:pathology
chemical_list: C439263:AM 1241; D001095:Arachidonic Acids; D063386:Cannabinoid Receptor Agonists; D002186:Cannabinoids; C469526:Cnr2 protein, rat; D052246:Cyclooxygenase 2 Inhibitors; D012333:RNA, Messenger; D043884:Receptor, Cannabinoid, CB1; D043885:Receptor, Cannabinoid, CB2; D013876:Thiophenes; C119325:arachidonyl-2-chloroethylamide; C064874:DuP 697; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat