title: Screening biomarkers of prostate cancer by integrating microRNA and mRNA microarrays.
abstract: OBJECTIVE
  In this study, we screened microRNA (miRNA) target genes of prostate cancer by integrating miRNA and mRNA expression profiles after target prediction and performed function enrichment analysis for selected candidate genes.
  
  
  METHODS
  The miRNA expression profile (GSE36802) and mRNA expression profile (GSE36801) were downloaded from the Gene Expression Omnibus database. We processed data and identified the differentially expressed miRNAs and mRNAs with R packages. Verified targets of miRNAs were identified through miRecods and miRTarBase. Then, software of Search Tool for the Retrieval of Interacting Genes was used to construct the interaction network of target genes. Finally, we performed function enrichment analysis for genes in the interaction network with the Functional Classification Tool.
  
  
  RESULTS
  A total of 22 upregulated and 8 downregulated miRNAs were detected in this study, of which, hsa-mir-31 was the most overexpressed miRNA in prostate cancer. Both ITGA5 and RDX, two target genes of hsa-mir-31, were found to be differentially expressed from mRNA profiles by overexpressing hsa-mir-31. The cell adhesion molecule was found to be the most significant pathway enriched by ITGA5 and RDX.
  
  
  CONCLUSIONS
  Overexpression of hsa-mir-31 can be a significant marker to distinguish cancer tissues from benign tissues. The targets such as ITGA5 and RDX regulated by hsa-mir-31 are candidate genes of prostate cancer, which provide new treatment strategies for its gene therapy.
authors.affiliation: 1 Department of Urology, Second Affiliated Hospital, Third Military Medical University , Chongqing, China .
authors.forename: Jiayu
authors.initials: J
authors.lastname: Feng
authors.forename: Chibing
authors.initials: C
authors.lastname: Huang
authors.forename: Xinwei
authors.initials: X
authors.lastname: Diao
authors.forename: Minqi
authors.initials: M
authors.lastname: Fan
authors.forename: Pingxian
authors.initials: P
authors.lastname: Wang
authors.forename: Ya
authors.initials: Y
authors.lastname: Xiao
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhong
authors.forename: Ronghua
authors.initials: R
authors.lastname: Wu
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D006801:Humans; D008297:Male; D008403:Mass Screening / Q000379:methods; D035683:MicroRNAs / Q000235:genetics* / Q000302:isolation & purification; D046228:Microarray Analysis; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D018511:Systems Integration
chemical_list: D014408:Biomarkers, Tumor; C534612:MIRN31 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger