title: Overexpression of UHRF1 is significantly associated with poor prognosis in laryngeal squamous cell carcinoma.
abstract: The ubiquitin-like with PHD and ring finger domains 1 (UHRF1) has been reported to be essential for cell proliferation and plays a critical role in the development and progression of many human carcinomas. However, its clinical and prognostic significance in laryngeal squamous cell carcinoma (LSCC) remains unclear. In the current study, 60 patients with LSCC were studied. UHRF1 expression at mRNA and protein levels was detected by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and immunohistochemistry, respectively, in both tissues of LSCC and corresponding adjacent normal larynx tissues. Statistical analyses were conducted to test the correlations between UHRF1 expression, clinicopathological parameters, and prognosis. qRT-PCR showed that the expression of UHRF1 mRNA in LSCC tissues was significantly higher than that in adjacent normal larynx tissues (P < 0.001). By immunohistochemistry, overexpression of UHRF1 protein was found in 78.3% (47/60) of the LSCC tissues, while there was negative expression in adjacent normal larynx tissues. Furthermore, increased expression of UHRF1 had remarkably positive relationships with smoking (P < 0.001), advanced T stage (P = 0.005) and clinical stage (P = 0.044), poor histological differentiation (P = 0.048), while there was no correlations between UHRF1, and sex, age and lymph node metastasis (P > 0.05). Overexpression of UHRF1 was also associated with worse overall survival examined by Kaplan-Meier method (P = 0.036). Multivariate analysis showed that increased expression of UHRF1 was an independent prognostic factor for LSCC (P = 0.013). Therefore, overexpression of UHRF1 may play an important role in the development and progression of LSCC, and UHRF1 might be a useful biomarker for the prognosis of LSCC.
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Chongqing, 400016, People's Republic of China.
authors.forename: Jing-Ting
authors.initials: JT
authors.lastname: Pi
authors.forename: Ye
authors.initials: Y
authors.lastname: Lin
authors.forename: Qiu
authors.initials: Q
authors.lastname: Quan
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Chen
authors.forename: Li-Zhu
authors.initials: LZ
authors.lastname: Jiang
authors.forename: Wang
authors.initials: W
authors.lastname: Chi
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics; D022762:CCAAT-Enhancer-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology*; D018450:Disease Progression; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D044767:Ubiquitin-Protein Ligases
chemical_list: D014408:Biomarkers, Tumor; D022762:CCAAT-Enhancer-Binding Proteins; D012333:RNA, Messenger; C406364:UHRF1 protein, human; D044767:Ubiquitin-Protein Ligases