title: Glucocorticoid paradoxically recruits adipose progenitors and impairs lipid homeostasis and glucose transport in mature adipocytes.
abstract: Chronic treatment with glucocorticoids increases the mass of adipose tissue and promotes metabolic syndrome. However little is known about the molecular effects of dexamethasone on adipose biology. Here, we demonstrated that dexamethasone induces progenitor cells to undergo adipogenesis. In the adipogenic pathway, at least two cell types are found: cells with the susceptibility to undergo staurosporine-induced adipose conversion and cells that require both staurosporine and dexamethasone to undergo adipogenesis. Dexamethasone increased and accelerated the expression of main adipogenic genes such as pparg2, cebpa and srebf1c. Also, dexamethasone altered the phosphorylation pattern of C/EBPβ, which is an important transcription factor during adipogenesis. Dexamethasone also had effect on mature adipocytes mature adipocytes causing the downregulation of some lipogenic genes, promoted a lipolysis state, and decreased the uptake of glucose. These paradoxical effects appear to explain the complexity of the action of glucocorticoids, which involves the hyperplasia of adipose cells and insulin resistance.
authors.affiliation: Department of Cell Biology, Center for Research and Advanced Studies-IPN, Avenida Instituto Politécnico Nacional 2508, Colonia San Pedro Zacatenco, Mexico City, MExico CP 07360.
authors.forename: Jorge-Tonatiuh
authors.initials: JT
authors.lastname: Ayala-Sumuano
authors.forename: Cristina
authors.initials: C
authors.lastname: Velez-delValle
authors.forename: Alicia
authors.initials: A
authors.lastname: Beltrán-Langarica
authors.forename: Meytha
authors.initials: M
authors.lastname: Marsch-Moreno
authors.forename: Claudia
authors.initials: C
authors.lastname: Hernandez-Mosqueira
authors.forename: Walid
authors.initials: W
authors.lastname: Kuri-Harcuch
mesh_terms: D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism*; D050156:Adipogenesis / Q000187:drug effects / Q000235:genetics; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D005938:Glucocorticoids / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D006706:Homeostasis / Q000187:drug effects*; D050356:Lipid Metabolism / Q000187:drug effects*; D051379:Mice; D010766:Phosphorylation; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D022782:CCAAT-Enhancer-Binding Protein-beta; D005938:Glucocorticoids; D005947:Glucose