title: PAMAM conjugated chitosan through naphthalimide moiety for enhanced gene transfection efficiency.
abstract: Development of efficient and safe gene carrier is the main hurdle for successful gene therapy till date. Poor water solubility and low transfection efficiency of chitosan are the main drawbacks to be efficient gene carrier for successful gene therapy. In this work, PAMAM conjugated chitosan was prepared through naphthalimide moiety by simple substitution reaction. The synthesis of the chitosan conjugates was confirmed by FTIR, (1)H NMR and XRD analyses. The conjugates showed enhanced DNA binding capability compared to that of unmodified chitosan. Moreover, the conjugates showed minimal cytotoxicity compared to that of polyethyleneimine (PEI, 25 kDa) and also showed good blood compatibility with negligible haemolysis. The transfection efficiency of the conjugate was significantly increased compared to that of unmodified chitosan and it also surpassed the transfection efficiency by PEI. Therefore, PAMAM conjugated chitosan can be used safely as alternate efficient gene delivery vector in gene therapy.
authors.affiliation: Department of Polymer Science & Technology, University of Calcutta, 92, A.P.C. Road, Kolkata 700009, West Bengal, India.
authors.forename: Kishor
authors.initials: K
authors.lastname: Sarkar
authors.forename: P P
authors.initials: PP
authors.lastname: Kundu
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D002021:Buffers; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D050091:Dendrimers / Q000737:chemistry*; D003850:Deoxyribonuclease I / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D006367:HeLa Cells; D006801:Humans; D053644:Naphthalimides / Q000737:chemistry*; D010316:Particle Size; D014162:Transfection / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D002021:Buffers; D050091:Dendrimers; D004337:Drug Carriers; D053644:Naphthalimides; C104700:PAMAM Starburst; D004247:DNA; D048271:Chitosan; D003850:Deoxyribonuclease I
keywords: Chitosan; Cytotoxicity; In vitro; PAMAM dendrimer; Transfection