title: Kordia aquimaris sp. nov., a zeaxanthin-producing member of the family Flavobacteriaceae isolated from surface seawater, and emended description of the genus Kordia.
abstract: A Gram-staining-negative, strictly aerobic, rod-shaped, non-flagellated, non-spore-forming and gliding marine bacterium designated strain CC-AMZ-301(T) was isolated from coastal surface seawater near Taichung harbour, Taiwan. Strain CC-AMZ-301(T) predominantly synthesized zeaxanthin and thus formed yellow colonies on marine agar. The novel strain showed high pairwise 16S rRNA gene sequence similarity to Kordia periserrulae IMCC1412(T) (95.0 %), K. algicida KCTC 8814P(T) (94.4 %) and K. antarctica IMCC3317(T) (94.2 %), and formed a phylogenetic lineage tightly associated with species of the genus Kordia. The polar lipid profile of strain CC-AMZ-301(T) consisted of three unidentified lipids, three unidentified aminolipids and one unidentified phospholipid. The major fatty acids (>5 % of total) were iso-C17 : 0 3-OH, iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 9 (iso-C17 : 1ω9c and/or 10-methyl C16 : 0). The DNA G+C content was 36.4 mol%. Menaquinone 6 (MK-6) was the predominant respiratory quinone. The major polyamine was the triamine sym-homospermidine. Based on evidence from the present polyphasic study, strain CC-AMZ-301(T) is proposed to represent a novel species of the genus Kordia, for which the name Kordia aquimaris sp. nov. is proposed; the type strain is CC-AMZ-301(T) ( = JCM 18556(T) = BCRC 80464(T)). An emended description of the genus Kordia is also proposed.
authors.affiliation: Department of Soil & Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Asif
authors.initials: A
authors.lastname: Hameed
authors.affiliation: Department of Soil & Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Mariyam
authors.initials: M
authors.lastname: Shahina
authors.affiliation: Department of Soil & Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Shih-Yao
authors.initials: SY
authors.lastname: Lin
authors.affiliation: Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
authors.forename: Jang-Cheon
authors.initials: JC
authors.lastname: Cho
authors.affiliation: Department of Soil & Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Wei-An
authors.initials: WA
authors.lastname: Lai
authors.affiliation: Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan.|Department of Soil & Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Chiu-Chung
authors.initials: CC
authors.lastname: Young
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013095:Spermidine / Q000031:analogs & derivatives / Q000737:chemistry; D013624:Taiwan; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D024341:Xanthophylls / Q000378:metabolism*; D065146:Zeaxanthins
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024341:Xanthophylls; D065146:Zeaxanthins; C007961:sym-homospermidine; D024482:Vitamin K 2; C042455:menaquinone 6; D013095:Spermidine