title: Rice black-streaked dwarf virus genome segment S5 is a bicistronic mRNA in infected plants.
abstract: Rice black-streaked dwarf virus (RBSDV) is a recognized member of the genus Fijivirus, family Reoviridae. Genome segment S5 has a putative second ORF partially overlapping the major ORF but in a different reading frame. This putative ORF is present in a published sequence and in two Chinese isolates now sequenced. Antibodies were raised against purified P5-1 and P5-2 fusion proteins expressed in a prokaryotic system. In western blots, these antibodies reacted with proteins of about 106 and 27 kDa, respectively, as predicted by sequence analysis. In immunoelectron microscopy, antibodies to P5-1 reacted with viroplasms, indicating that P5-1 is a component of viroplasms, but no labeling was observed with P5-2 antisera. Northern blot assays showed that the genome segment S5 was transcribed as a single mRNA with no subgenomic RNA. These results show that S5 is functionally bicistronic in infected plants. Possible translational mechanisms for P5-2 are discussed.
authors.affiliation: College of Bio-Safety Science and Technology, Hunan Agricultural University, Changsha, 410128, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Heng-Mu
authors.initials: HM
authors.lastname: Zhang
authors.forename: Lu
authors.initials: L
authors.lastname: Ying
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Ming-Fang
authors.initials: MF
authors.lastname: Lv
authors.forename: Li
authors.initials: L
authors.lastname: Xie
authors.forename: Pei-Pei
authors.initials: PP
authors.lastname: Li
authors.forename: Xiao-Ya
authors.initials: XY
authors.lastname: Liu
authors.forename: Dai
authors.initials: D
authors.lastname: Liang-Ying
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Chen
mesh_terms: D000914:Antibodies, Viral / Q000378:metabolism; D015152:Blotting, Northern; D005796:Genes; D006801:Humans; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012275:Oryza / Q000821:virology*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012087:Reoviridae / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013194:Staining and Labeling / Q000379:methods; D014158:Transcription, Genetic
chemical_list: D000914:Antibodies, Viral; D012333:RNA, Messenger; D012367:RNA, Viral