title: [Substantiation of optimum condition of parainfluenza-3 virus cultivation for production of vaccines on the finite cell line].
abstract: Research is performed for investigation of cultivation peculiarities of virus parainfluenza-3 on different cell cultures and optimization of methods of virus infection of these cultures with PI-3 virus in the process of development of vaccines. Influence of a seed lot on the formation rate of a cellular monolayer of continuous cell cultures PT-80, LEK, KSTand T-1 is defined, it is established that the seed lot of 250,000 cells in 1 ml provides optimum concentration for formation of a full monolayer within the time limits of 48-72 hours. The most sensitive cell cultures (PT-80) for cultivation of parainfluenza-3 virus are selected; an optimal CCID of virus PI-3(0,1 TCD/ml), whereby we can observe the maximal accumulation of a virus within 2-4 day after infecting cell cultures, is defined.
authors.forename: D
authors.initials: D
authors.lastname: Bekhzadpur
authors.forename: R V
authors.initials: RV
authors.lastname: Belousova
authors.forename: I V
authors.initials: IV
authors.lastname: Ivanov
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D006801:Humans; D022241:Parainfluenza Vaccines; D010224:Parainfluenza Virus 3, Human / Q000254:growth & development* / Q000276:immunology; D010253:Respirovirus Infections / Q000276:immunology / Q000378:metabolism* / Q000517:prevention & control
chemical_list: D022241:Parainfluenza Vaccines