title: Impact of molecular flexibility on double polymorphism, solid solutions and chiral discrimination during crystallization of diprophylline enantiomers.
abstract: The polymorphic behavior of racemic and enantiopure diprophylline (DPL), a chiral derivative of theophylline marketed as a racemic solid, has been investigated by combining differential scanning calorimetry, powder X-ray diffraction, hot-stage microscopy and single-crystal X-ray experiments. The pure enantiomers were obtained by a chemical synthesis route, and additionally an enantioselective crystallization procedure was developed. The binary phase diagram between the DPL enantiomers was constructed and revealed a double polymorphism (i.e., polymorphism both of the racemic mixture and of the pure enantiomer). The study of the various equilibria in this highly unusual phase diagram revealed a complex situation since mixtures of DPL enantiomers can crystallize either as a stable racemic compound, a metastable conglomerate, or two distinct metastable solid solutions. Crystal structure analysis revealed that the DPL molecules adopt different conformations in the crystal forms suggesting that the conformational degrees of freedom of the substituent that carries the only two H-bond donor groups might be related to the versatile crystallization behavior of DPL. The control of these equilibria and the use of a suitable solvent allowed the design of an efficient protocol for the preparative resolution of racemic DPL via preferential crystallization. Therefore, the resolution of DPL enantiomers despite the existence of a racemic compound stable at any temperature demonstrates that the detection of a stable conglomerate is not mandatory for the implementation of preferential crystallization.
authors.affiliation: Unité de Cristallogenèse, SMS, EA 3233, Université de Rouen , PRES Normandie Université , F-76821 Mont-Saint-Aignan Cedex, France.
authors.forename: Clément
authors.initials: C
authors.lastname: Brandel
authors.forename: Youness
authors.initials: Y
authors.lastname: Amharar
authors.forename: Judith M
authors.initials: JM
authors.lastname: Rollinger
authors.forename: Ulrich J
authors.initials: UJ
authors.lastname: Griesser
authors.forename: Yohann
authors.initials: Y
authors.lastname: Cartigny
authors.forename: Samuel
authors.initials: S
authors.lastname: Petit
authors.forename: Gérard
authors.initials: G
authors.lastname: Coquerel
mesh_terms: D002152:Calorimetry, Differential Scanning; D003460:Crystallization; D004400:Dyphylline / Q000737:chemistry*; D015394:Molecular Structure; D011487:Protein Conformation; D013237:Stereoisomerism; D014961:X-Ray Diffraction
chemical_list: D004400:Dyphylline