title: Anticancer activities of an antimicrobial peptide derivative of Ixosin-B amide.
abstract: In nature, antimicrobial peptides (AMPs) represent the first line of defense against infection by pathogens; thus, they are generally good candidates for the development of antimicrobial agents. Recently, we reported two potent antimicrobial peptides, KWLRRVWRWWR-amide (MAP-04-03) and KRLRRVWRRWR-amide (MAP-04-04), which were derived from a fragment of Ixosin-B-amide (KSDVRRWRSRY). Since some cationic AMPs exhibited cytotoxic activity against cancer cells, in the current study, we further investigated the anticancer activity of these potent antimicrobial peptides by antiproliferative assays and wound-healing assays, and the effect of peptide on the cytoskeleton alteration and cell morphology were analyzed by confocal microscopy. Results indicated that MAP-04-03 not only exhibited inhibitory effects on the proliferation (IC50=61.5 μM) and on the cell migration of MCF-7 breast cancer cells (at a concentration of 5 μM), but also affected the cytoskeleton at the concentration of 25 μM. These results demonstrated that MAP-04-03 can serve as a lead peptide analog for developing potent anticancer agents.
authors.affiliation: Department of Chemistry, Tunghai University, Taichung, Taiwan, ROC.
authors.forename: Yu-Cheng
authors.initials: YC
authors.lastname: Hsiao
authors.forename: Kai-Shiuan
authors.initials: KS
authors.lastname: Wang
authors.forename: Shu-Huai
authors.initials: SH
authors.lastname: Tsai
authors.forename: Wei-Ting
authors.initials: WT
authors.lastname: Chao
authors.forename: Feng-Di T
authors.initials: FD
authors.lastname: Lung
mesh_terms: D000577:Amides / Q000737:chemistry; D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000633:toxicity; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000633:toxicity; D060829:Arthropod Proteins / Q000737:chemistry* / Q000633:toxicity; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects; D006801:Humans; D061986:MCF-7 Cells; D017433:Protein Structure, Secondary
chemical_list: D000577:Amides; D023181:Antimicrobial Cationic Peptides; D000970:Antineoplastic Agents; D060829:Arthropod Proteins; C584460:ixosin-B peptide, Ixodes sinensis
keywords: Anticancer activity; Antimicrobial activity; Antimicrobial peptides; Ixosin-B