title: Hyperspectral fluorescence microfluidic (HFM) microscopy.
abstract: We present an imaging system that collects hyperspectral images of cells travelling through a microfluidic channel. Using a single monochrome camera and a linear variable bandpass filter (LVF), the system captures a bright field image and a set of hyperspectral fluorescence images for each cell. While the bandwidth of the LVF is 20 nm, we have demonstrated that we can determine the peak wavelength of a fluorescent object's emission spectrum with an accuracy of below 3 nm. In addition, we have used this system to capture fluorescence spectra of individual spatially resolved cellular organelles and to spectrally resolve multiple fluorophores in individual cells.
authors.affiliation: Rowland Institute at Harvard, Harvard University, 100 E. Land Boulevard, Cambridge MA, USA.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Di Caprio
authors.forename: Diane
authors.initials: D
authors.lastname: Schaak
authors.forename: Ethan
authors.initials: E
authors.lastname: Schonbrun
keywords: (110.4234) Multispectral and hyperspectral imaging; (170.3880) Medical and biological imaging; (180.2520) Fluorescence microscopy