title: Digoxigenin-labeled in situ hybridization for the detection of Streptococcus suis DNA in polyserositis and a comparison with biotinylated in situ hybridization.
abstract: The objective of this study was to develop digoxigenin-labeled in situ hybridization (ISH) for the detection of Streptococcus suis in naturally infected pigs with polyserositis and to compare it with biotinylated ISH. Digoxigenin-labeled hybridization signals for S. suis were observed in cells that had infiltrated the fibrous polyserositis and microcolonies in the blood vessels. Mock hybridization showed no hybridization signals for endogenous digoxigenin. Biotinylated hybridization signals for S. suis were observed in cells that had infiltrated the fibrous polyserositis. However, similar hybridization signals were also observed in the fibrous inflammatory area using mock hybridization for endogenous biotin. The present study demonstrated that digoxigenin-labeled ISH is a valuable diagnostic tool for specific detection of S. suis in polyserositic tissues without nonspecific reactions compared with biotinylated ISH.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Ikjae
authors.initials: I
authors.lastname: Kang
authors.forename: Hwi Won
authors.initials: HW
authors.lastname: Seo
authors.forename: Changhoon
authors.initials: C
authors.lastname: Park
authors.forename: Yeonsu
authors.initials: Y
authors.lastname: Oh
authors.forename: Jeehoon
authors.initials: J
authors.lastname: Lee
authors.forename: Ok Heui
authors.initials: OH
authors.lastname: You
authors.forename: Sung-Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: Marcelo
authors.initials: M
authors.lastname: Gottschalk
authors.forename: Chanhee
authors.initials: C
authors.lastname: Chae
mesh_terms: D000818:Animals; D001710:Biotin; D004269:DNA, Bacterial / Q000235:genetics; D004076:Digoxigenin; D010505:Familial Mediterranean Fever / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D017403:In Situ Hybridization / Q000379:methods / Q000662:veterinary*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013290:Streptococcal Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D017011:Streptococcus suis / Q000235:genetics / Q000302:isolation & purification*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D001710:Biotin; D004076:Digoxigenin