title: X chromosomal recombination--a family study analyzing 26 X-STR Loci in Chinese Han three-generation pedigrees.
abstract: The aim of this study is to investigate genetic linkage and recombination fractions of 26 X chromosomal (X-STR) loci with two multiplex PCR systems (MX15-STR and MX12-STR). MX15-STR (including DXS7133, DXS6801, DXS981, DXS6809, DXS7424, DXS6789, DXS9898, DXS7132, GATA165B12, DXS101, DXS10075, DXS6800, GATA31E08, DXS10074, and DXS10079) and MX12-STR (including DXS6854, DXS9902, DXS6800, GATA172D05, DXS7423, HPRTB, DXS6807, DXS6803, DXS6804, DXS6799, DXS8378, and DXS8377) were successful analyzed on 206 two-generation families with two or more children and 33 three-generation families with 72 grandsons. Segregation analysis and calculation of recombination fractions between pairs of markers were performed. Linkage analysis of pairs of markers showed that there existed significant linkage (maximum LOD scores >2.0) within the physical distance of 48.5 Mb. Recombination events could be observed within the clusters of closed linked makers spanning <1.0 Mb. These results indicate that close cluster X-STRs used and recombination fractions of the selected loci will be very useful for biostatistical calculations in complex kinship analysis.
authors.affiliation: Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, P. R. China; Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Sciences, Ministry of Justice, Shanghai, P. R. China.
authors.forename: Qiu-Ling
authors.initials: QL
authors.lastname: Liu
authors.forename: Ze-Dong
authors.initials: ZD
authors.lastname: Li
authors.forename: Cheng-Tao
authors.initials: CT
authors.lastname: Li
authors.forename: Hu
authors.initials: H
authors.lastname: Zhao
authors.forename: Ye-Da
authors.initials: YD
authors.lastname: Wu
authors.forename: Quan
authors.initials: Q
authors.lastname: Li
authors.forename: De-Jian
authors.initials: DJ
authors.lastname: Lu
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics*; D041321:Chromosomes, Human, X / Q000235:genetics*; D005260:Female; D056426:Genetic Loci; D006801:Humans; D008297:Male; D060885:Multiplex Polymerase Chain Reaction; D009154:Mutation; D010375:Pedigree; D011995:Recombination, Genetic; D055815:Young Adult
keywords: Chinese Han; Linkage; Multiplex PCR; Recombination; X chromosomal