title: [β-amyloid protein up-regulates the expression of the receptor for advanced glycation end products by increasing ROS production].
abstract: OBJECTIVE
  To investigate the mechanism of β-amyloid protein (Aβ) in regulating the expression of the receptor for advanced glycation end products (RAGE).
  
  
  METHODS
  Aβ1-40 was injected into the bilateral hippocampus of rats, and 3 weeks later, the levels of reactive oxygen species (ROS) production were detected by flow cytometry. The expressions of RAGE, reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (gp9l(phox) and p47(phox)), nuclear factor-κB (NF-κB), and inhibitor of κB (IκB) were measured by Western blotting.
  
  
  RESULTS
  Injection of Aβ1-40 caused a significant increase in the expressions of RAGE, gp9l(phox), p47(phox), phospho-p47(phox), phospho-IκBα, NF-κB and phospho-NF-κB in rat hippocampus but decreased the level of IκBα. Aβ1-40 injection also resulted in a significantly increased content of ROS in the hippocampus of the rats.
  
  
  CONCLUSIONS
  Aβ up-regulates the expression of RAGE in rat hippocampus via NADPH/ ROS/NF-κB signaling pathway.
authors.affiliation: Bioreactor and Protein Drug Research and Development Center of Hebei Universities, Hebei Chemical and Pharmaceutical College, Shijiazhuang 050026, China. E-mail: kongweina0219@163.com.
authors.forename: Weina
authors.initials: W
authors.lastname: Kong
authors.forename: Jia
authors.initials: J
authors.lastname: Zhang
authors.forename: Weijuan
authors.initials: W
authors.lastname: Gao
authors.forename: Qingtao
authors.initials: Q
authors.lastname: Liu
authors.forename: Liming
authors.initials: L
authors.lastname: Zhou
authors.forename: Xiqing
authors.initials: X
authors.lastname: Chai
mesh_terms: D016229:Amyloid beta-Peptides / Q000009:adverse effects*; D000818:Animals; D006624:Hippocampus / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress; D010446:Peptide Fragments / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000378:metabolism*; D015398:Signal Transduction; D015854:Up-Regulation
chemical_list: D016229:Amyloid beta-Peptides; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D010446:Peptide Fragments; D017382:Reactive Oxygen Species; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; C078592:amyloid beta-protein (1-40); D009249:NADP; C500593:Cybb protein, rat; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1