title: Cell culture and characterization of cross-linked poly(vinyl alcohol)-g-starch 3D scaffold for tissue engineering.
abstract: The research goal of this experiment is chemically to cross-link poly(vinyl alcohol) (PVA) and starch to form a 3D scaffold that is effective water absorbent, has a stable structure, and supports cell growth. PVA and starch can be chemically cross-linked to form a PVA-g-starch 3D scaffold polymer, as observed by Fourier transform infrared spectroscopy (FTIR), with an absorbency of up to 800%. Tensile testing reveals that, as the amount of starch increases, the strength of the 3D scaffold strength reaches 4×10(-2) MPa. Scanning electron microscope (SEM) observations of the material reveal that the 3D scaffold is highly porous formed using a homogenizer at 500 rpm. In an enzymatic degradation, the 3D scaffold was degraded by various enzymes at a rate of up to approximately 30-60% in 28 days. In vitro tests revealed that cells proliferate and grow in the 3D scaffold material. Energy dispersive spectrometer (EDS) analysis further verified that the bio-compatibility of this scaffold.
authors.affiliation: Department of Biological Science and Technology, I-Shou University, Kaohsiung 82445, Taiwan, ROC. wenchuan@isu.edu.tw
authors.forename: Wen-Chuan
authors.initials: WC
authors.lastname: Hsieh
authors.forename: Jiun-Jia
authors.initials: JJ
authors.lastname: Liau
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D011142:Polyvinyl Alcohol / Q000737:chemistry* / Q000494:pharmacology*; D016062:Porosity; D044968:Regenerative Medicine; D013718:Tensile Strength; D023822:Tissue Engineering / Q000191:economics; D054457:Tissue Scaffolds / Q000737:chemistry* / Q000191:economics
chemical_list: D001672:Biocompatible Materials; D011142:Polyvinyl Alcohol
keywords: 3D scaffold; Biodegradation; Cell culture; Cross-linking; PVA-g-starch