title: Structure-based investigation on the interaction of perfluorinated compounds with human liver fatty acid binding protein.
abstract: Perfluorinated compounds (PFCs) are known to accumulate in liver and induce hepatotoxicity on experimental animals. Liver fatty acid binding protein (L-FABP) is expressed highly in hepatocytes and binds fatty acids. PFCs may bind with FABP and change their ADME and toxicity profile. In the present study, the binding interaction of 17 structurally diverse PFCs with human L-FABP was investigated to assess their potential disruption effect on fatty acid binding. The binding affinity of twelve perfluorinated carboxylic acids (PFCAs), as determined by fluorescence displacement assay, increased significantly with their carbon number from 4 to 11, and decreased slightly when the number was over 11. The three perfluorinated sulfonic acids (PFSAs) displayed comparable affinity, but no binding was detected for the two fluorotelomer alcohols. Circular dichroism results showed that PFC binding induced distinctive structural changes of the protein. Molecular docking revealed that the driving forces for the binding of PFCs with FABP were predominantly hydrophobic and hydrogen-bonding interactions, and the binding geometry was dependent on both the size and rigidity of the PFCs. Based on the binding constant obtained in this work, the possibility of in vivo competitive displacement of fatty acids from FABP by PFCs was estimated.
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences , P.O. Box 2871, 18 Shuangqing Road, Beijing 100085, China.
authors.forename: Lianying
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Ren
authors.forename: Liang-Hong
authors.initials: LH
authors.lastname: Guo
mesh_terms: D017738:Alkanesulfonic Acids / Q000737:chemistry / Q000378:metabolism*; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D005466:Fluorocarbons / Q000737:chemistry / Q000378:metabolism*; D006801:Humans
chemical_list: D017738:Alkanesulfonic Acids; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D005466:Fluorocarbons