title: Middle East respiratory syndrome coronavirus spike protein delivered by modified vaccinia virus Ankara efficiently induces virus-neutralizing antibodies.
abstract: Middle East respiratory syndrome coronavirus (MERS-CoV) has recently emerged as a causative agent of severe respiratory disease in humans. Here, we constructed recombinant modified vaccinia virus Ankara (MVA) expressing full-length MERS-CoV spike (S) protein (MVA-MERS-S). The genetic stability and growth characteristics of MVA-MERS-S make it a suitable candidate vaccine for clinical testing. Vaccinated mice produced high levels of serum antibodies neutralizing MERS-CoV. Thus, MVA-MERS-S may serve for further development of an emergency vaccine against MERS-CoV.
authors.affiliation: Institute for Infectious Diseases and Zoonoses, LMU University of Munich, Munich, Munich, Germany.
authors.forename: Fei
authors.initials: F
authors.lastname: Song
authors.forename: Robert
authors.initials: R
authors.lastname: Fux
authors.forename: Lisette B
authors.initials: LB
authors.lastname: Provacia
authors.forename: Asisa
authors.initials: A
authors.lastname: Volz
authors.forename: Markus
authors.initials: M
authors.lastname: Eickmann
authors.forename: Stephan
authors.initials: S
authors.lastname: Becker
authors.forename: Albert D M E
authors.initials: AD
authors.lastname: Osterhaus
authors.forename: Bart L
authors.initials: BL
authors.lastname: Haagmans
authors.forename: Gerd
authors.initials: G
authors.lastname: Sutter
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D017934:Coronavirus / Q000235:genetics / Q000276:immunology*; D004337:Drug Carriers / Q000008:administration & dosage*; D005822:Genetic Vectors / Q000008:administration & dosage; D042822:Genomic Instability; D051379:Mice; D008807:Mice, Inbred BALB C; D064370:Spike Glycoprotein, Coronavirus / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D004337:Drug Carriers; D064370:Spike Glycoprotein, Coronavirus; D014614:Vaccines, Synthetic; D014765:Viral Vaccines