title: Avidity of antibodies against HSV-2 and risk to neonatal transmission among Mexican pregnant women.
abstract: OBJECTIVE
  To determine HSV-2 seroprevalence, risk factors, and antibody avidity among a sample of Mexican pregnant women.
  
  
  METHODS
  The avidity test was standardized with different urea concentrations and incubation times; the cut-off point was calculated to determine the low avidity (early infection). IgG antibodies against HSV-2 were detected from pregnant and postpartum women from Morelos, Mexico, and the avidity test was performed to positive samples. Multivariate regression logistic analysis was employed to evaluate demographic and sexual behavior characteristics associated with HSV-2 infection.
  
  
  RESULTS
  HSV-2 seroprevalence among Mexican women analyzed was 14.5% (333/2300), demographic factors (location of General Hospital, age, education level, and civil status), and risky sexual behaviors (STI self-report and number of sexual partners during last year) were associated with HSV-2 infection. Seventeen women were detected with low avidity antibodies (early infection) with a cut-off point of 66.1%.
  
  
  CONCLUSIONS
  HSV-2 infection was common among this group of women from Mexico; the avidity test detected women with recent infections, and these women were more likely to transmit HSV-2 to their neonates. Neonatal herpes has no epidemiological surveillance, the disease could be overlooked, and so more studies are needed to estimate the magnitude of neonatal infection.
authors.affiliation: Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Avenida Universidad No. 655, Colonia Santa María Ahuacatitlán, Cerrada Los Pinos y Caminera, CP 62100, Cuernavaca, Morelos, Mexico.
authors.forename: Antonia
authors.initials: A
authors.lastname: Herrera-Ortiz
authors.forename: Carlos Jesús
authors.initials: CJ
authors.lastname: Conde-Glez
authors.forename: Dayana Nicté
authors.initials: DN
authors.lastname: Vergara-Ortega
authors.forename: Santa
authors.initials: S
authors.lastname: García-Cisneros
authors.forename: Ma Leonidez
authors.initials: ML
authors.lastname: Olamendi-Portugal
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Sánchez-Alemán
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D000915:Antibody Affinity; D005260:Female; D006558:Herpes Genitalis / Q000276:immunology* / Q000635:transmission* / Q000821:virology; D018258:Herpesvirus 2, Human / Q000276:immunology*; D006801:Humans; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000276:immunology* / Q000821:virology*; D012307:Risk Factors; D016036:Seroepidemiologic Studies
chemical_list: D000914:Antibodies, Viral