title: Forming a complex with MHC class I molecules interferes with mouse CD1d functional expression.
abstract: CD1d molecules are structurally similar to MHC class I, but present lipid antigens as opposed to peptides. Here, we show that MHC class I molecules physically associate with (and regulate the functional expression of) mouse CD1d on the surface of cells. Low pH (3.0) acid stripping of MHC class I molecules resulted in increased surface expression of murine CD1d on antigen presenting cells as well as augmented CD1d-mediated antigen presentation to NKT cells. Consistent with the above results, TAP1-/- mice were found to have a higher percentage of type I NKT cells as compared to wild type mice. Moreover, bone marrow-derived dendritic cells from TAP1-/- mice showed increased antigen presentation by CD1d compared to wild type mice. Together, these results suggest that MHC class I molecules can regulate NKT cell function, in part, by masking CD1d.
authors.affiliation: Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana, USA.
authors.forename: Renukaradhya J
authors.initials: RJ
authors.lastname: Gourapura
authors.forename: Masood A
authors.initials: MA
authors.lastname: Khan
authors.forename: Richard M
authors.initials: RM
authors.lastname: Gallo
authors.forename: Daniel
authors.initials: D
authors.lastname: Shaji
authors.forename: Jianyun
authors.initials: J
authors.lastname: Liu
authors.forename: Randy R
authors.initials: RR
authors.lastname: Brutkiewicz
mesh_terms: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D055610:Antigens, CD1d / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003713:Dendritic Cells; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D016130:Immunophenotyping; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D018345:Mice, Knockout; D055611:Natural Killer T-Cells / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding
chemical_list: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters; D055610:Antigens, CD1d; D015395:Histocompatibility Antigens Class I; C486171:Tap1 protein, mouse