title: Effect of unconventional carbon sources on biosurfactant production and its application in bioremediation.
abstract: The potential of an alkaliphilic bacterium Klebsiella sp. strain RJ-03, to utilize different unconventional carbon sources for the production of biosurfactant was evaluated. The biosurfactant produced using corn powder, potato peel powder, Madhuca indica and sugarcane bagasse containing medium, exhibited significantly higher viscosity and maximum reduction in surface tension as compared to other substrates. Among several carbon substrates tested, production of biosurfactant was found to be the highest with corn powder (15.40 ± 0.21 g/l) as compared to others. The comparative chemical characterization of purified biosurfactant was done using advance analytical tools such as NMR, FT-IR, SEM, GPC, MALDI TOF-TOF MS, GC-MS, TG and DSC. Analyses indicated variation in the functional groups, monosaccharide composition, molecular mass, thermostability. Higher yield with cheaper raw materials, noteworthy stress tolerance of CP-biosurfactant toward pH and salt as well as compatibility with chemical surfactants and detergents revealed its potential for commercialization and application in bioremediation.
authors.affiliation: Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), G. B. Marg, Bhavnagar, Gujarat 364021, India.
authors.forename: Rakeshkumar M
authors.initials: RM
authors.lastname: Jain
authors.forename: Kalpana
authors.initials: K
authors.lastname: Mody
authors.forename: Nidhi
authors.initials: N
authors.lastname: Joshi
authors.forename: Avinash
authors.initials: A
authors.lastname: Mishra
authors.forename: Bhavanath
authors.initials: B
authors.lastname: Jha
mesh_terms: D001673:Biodegradation, Environmental; D001688:Biological Products / Q000737:chemistry / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism*; D032561:Cotton Fiber; D007220:Industrial Waste; D007709:Klebsiella / Q000378:metabolism*; D054327:Lubricants / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D009821:Oils / Q000302:isolation & purification / Q000378:metabolism; D013500:Surface Tension; D013501:Surface-Active Agents / Q000378:metabolism*; D014783:Viscosity
chemical_list: D001688:Biological Products; D007220:Industrial Waste; D054327:Lubricants; D009821:Oils; D013501:Surface-Active Agents; D002244:Carbon
keywords: Alkaliphilic bacteria; Bioremediation; Biosurfactant