title: Vig r 6, the cytokinin-specific binding protein from mung bean (Vigna radiata) sprouts, cross-reacts with Bet v 1-related allergens and binds IgE from birch pollen allergic patients' sera.
abstract: METHODS
  Birch pollen associated allergy to mung bean sprouts is caused by cross-reactivity between the birch pollen allergen Bet v 1 and the mung bean allergen Vig r 1. We aimed to determine the allergenicity of the cytokinin-specific binding protein from mung bean (Vig r 6), another allergen related to Bet v 1 with only 31% sequence identity.
  
  
  RESULTS
  Bet v 1, Gly m 4, Vig r 1, and Vig r 6 were produced in Escherichia coli. In an ELISA, 73 and 32% of Bet v 1-sensitized birch-allergic patients' sera (n = 60) showed IgE binding to Vig r 1 and Vig r 6, respectively. Of 19 patients who reported allergic reactions or had positive prick-to-prick tests to mung bean sprouts, 79% showed IgE binding to Vig r 1 and 63% showed IgE binding to Vig r 6. Bet v 1 completely inhibited IgE binding to both mung bean allergens. Vig r 6 showed partial cross-reactivity with Vig r 1 and activated basophils sensitized with mung bean allergic patients' sera.
  
  
  CONCLUSIONS
  We demonstrated IgE cross-reactivity despite low sequence identity between Vig r 6 and other Bet v 1-related allergens. Thus, IgE binding to Vig r 6 may contribute to birch pollinosis-associated mung bean sprout allergy.
authors.affiliation: Department of Pathophysiology and Allergy Research, Medical University of Vienna, Austria.
authors.forename: Eva Elisabeth
authors.initials: EE
authors.lastname: Guhsl
authors.forename: Gerlinde
authors.initials: G
authors.lastname: Hofstetter
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Hemmer
authors.forename: Christof
authors.initials: C
authors.lastname: Ebner
authors.forename: Stefan
authors.initials: S
authors.lastname: Vieths
authors.forename: Lothar
authors.initials: L
authors.lastname: Vogel
authors.forename: Heimo
authors.initials: H
authors.lastname: Breiteneder
authors.forename: Christian
authors.initials: C
authors.lastname: Radauer
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000485:Allergens / Q000235:genetics / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D052179:Antigens, Plant / Q000276:immunology*; D001491:Basophils / Q000276:immunology; D002648:Child; D003429:Cross Reactions / Q000276:immunology; D007887:Fabaceae / Q000276:immunology*; D005260:Female; D006801:Humans; D007106:Immune Sera; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000276:immunology; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012189:Retrospective Studies; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology*; D055815:Young Adult
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D007106:Immune Sera; D010940:Plant Proteins; D011994:Recombinant Proteins; C000597742:Vig r 6 allergen, Vigna radiata; C061312:Bet v 1 allergen, Betula; D007073:Immunoglobulin E
keywords: Bet v 1; CSBP; Food allergy; IgE cross-reactivity; Vig r 6