title: Metabolic study of grapevine leaves infected by downy mildew using negative ion electrospray--Fourier transform ion cyclotron resonance mass spectrometry.
abstract: Grapevine is of worldwide economic importance due to wine production. However, this culture is often affected by pathogens causing severe harvest losses. Understanding host-pathogen relationships may be a key to solve this problem. In this paper, we evaluate the direct flow injection by electrospray - Fourier transform ion cyclotron resonance mass spectrometry (MS) of leaf extracts as a rapid method for the study of grapevine response to downy mildew (Plasmopara viticola) attack. The comparison of MS profiles obtained from control and infected leaves of different levels of resistant grapevines highlights several classes of metabolites (mainly saccharides, acyl lipids, hydroxycinnamic acids derivatives and flavonoids) which are identified using high resolution MS and tandem MS (MS/MS). Statistical analyses of 19 markers show a clear segregation between inoculated and healthy samples. This study points out relative high levels of disaccharides, acyl lipids and glycerophosphoinositol in inoculated samples. Sulfoquinovosyl diacylglycerols also emerge as possible metabolites involved in plant defense.
authors.affiliation: Université de Lorraine, Laboratoire de Chimie et Physique-Approche Multi échelle des Milieux Complexes (LCP-A2MC), EA 4632, Institut Jean Barriol, Fédération de Recherche 2843, ICPM 1, Metz Technopole Cedex 03, France.
authors.forename: Loïc
authors.initials: L
authors.lastname: Becker
authors.forename: Anne
authors.initials: A
authors.lastname: Poutaraud
authors.forename: Grégory
authors.initials: G
authors.lastname: Hamm
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Muller
authors.forename: Didier
authors.initials: D
authors.lastname: Merdinoglu
authors.forename: Vincent
authors.initials: V
authors.lastname: Carré
authors.forename: Patrick
authors.initials: P
authors.lastname: Chaimbault
mesh_terms: D006017:Glycolipids / Q000737:chemistry; D007477:Ions / Q000737:chemistry; D044742:Peronospora / Q000302:isolation & purification; D018515:Plant Leaves / Q000378:metabolism / Q000382:microbiology; D025341:Principal Component Analysis; D021241:Spectrometry, Mass, Electrospray Ionization; D027843:Vitis / Q000378:metabolism* / Q000382:microbiology
chemical_list: D006017:Glycolipids; D007477:Ions; C013694:sulfoquinovosyl diglyceride
keywords: Downy mildew; Electrospray; Fourier transform ion cyclotron resonance; Grapevine; High resolution mass spectrometry; Metabolic profiling