title: Targeted quantitation of acetylated lysine peptides by selected reaction monitoring mass spectrometry.
abstract: Mass spectrometry (MS) allows for the large-scale identification of multiple peptide analytes in complex mixtures. However, the low abundance of acetylated peptides in the overall mixture requires an enrichment step. After enrichment, the resulting acetylated peptides of interest can be quantitated using selected reaction monitoring (SRM)-MS with stable isotope dilution. Here, we describe the enrichment of lysine acetylated peptides from typsin digested mouse liver mitochondria, and the targeted quantitation of a known lysine acetylation site in succinate dehydrogenase A using SRM-MS on a triple quadrupole instrument.
authors.affiliation: Buck Institute for Research on Aging, Novato, CA, USA.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Rardin
authors.forename: Jason M
authors.initials: JM
authors.lastname: Held
authors.forename: Bradford W
authors.initials: BW
authors.lastname: Gibson
mesh_terms: D000107:Acetylation; D000818:Animals; D002846:Chromatography, Affinity; D002853:Chromatography, Liquid / Q000379:methods*; D047468:Immunoprecipitation / Q000379:methods*; D007201:Indicator Dilution Techniques; D008239:Lysine / Q000378:metabolism*; D051379:Mice; D008930:Mitochondria, Liver / Q000201:enzymology*; D010446:Peptide Fragments / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D013385:Succinate Dehydrogenase / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010446:Peptide Fragments; D013385:Succinate Dehydrogenase; D008239:Lysine