title: Comparative genomics of Campylobacter concisus isolates reveals genetic diversity and provides insights into disease association.
abstract: BACKGROUND
  In spite of its association with gastroenteritis and inflammatory bowel diseases, the isolation of Campylobacter concisus from both diseased and healthy individuals has led to controversy regarding its role as an intestinal pathogen. One proposed reason for this is the presence of high genetic diversity among the genomes of C. concisus strains.
  
  
  RESULTS
  In this study the genomes of six C. concisus strains were sequenced, assembled and annotated including two strains isolated from Crohn's disease patients (UNSW2 and UNSW3), three from gastroenteritis patients (UNSW1, UNSWCS and ATCC 51562) and one from a healthy individual (ATCC 51561). The genomes of C. concisus BAA-1457 and UNSWCD, available from NCBI, were included in subsequent comparative genomic analyses. The Pan and Core genomes for the sequenced C. concisus strains consisted of 3254 and 1556 protein coding genes, respectively.
  
  
  CONCLUSIONS
  Genes were identified with specific conservation in C. concisus strains grouped by phenotypes such as invasiveness, adherence, motility and diseased states. Phylogenetic trees based on ribosomal RNA sequences and concatenated host-related pathways for the eight C. concisus strains were generated using the neighbor-joining method, of which the 16S rRNA gene and peptidoglycan biosynthesis grouped the C. concisus strains according to their pathogenic phenotypes. Furthermore, 25 non-synonymous amino acid changes with 14 affecting functional domains, were identified within proteins of conserved host-related pathways, which had possible associations with the pathogenic potential of C. concisus strains. Finally, the genomes of the eight C. concisus strains were compared to the nine available genomes of the well-established pathogen Campylobacter jejuni, which identified several important differences in the respiration pathways of these two species. Our findings indicate that C. concisus strains are genetically diverse, and suggest the genomes of this bacterium contain respiration pathways and modifications in the peptidoglycan layer that may play an important role in its virulence.
authors.affiliation: School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney 2052, NSW, Australia. H.Mitchell@unsw.edu.au.
authors.forename: Nandan P
authors.initials: NP
authors.lastname: Deshpande
authors.forename: Nadeem O
authors.initials: NO
authors.lastname: Kaakoush
authors.forename: Marc R
authors.initials: MR
authors.lastname: Wilkins
authors.forename: Hazel M
authors.initials: HM
authors.lastname: Mitchell
mesh_terms: D001422:Bacterial Adhesion / Q000235:genetics; D002167:Campylobacter / Q000235:genetics* / Q000302:isolation & purification; D002169:Campylobacter Infections / Q000382:microbiology*; D003424:Crohn Disease / Q000382:microbiology; D005759:Gastroenteritis / Q000382:microbiology; D063990:Gene Ontology; D016680:Genome, Bacterial; D023281:Genomics; D054884:Host-Pathogen Interactions; D006801:Humans; D010641:Phenotype; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D012329:RNA, Bacterial / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D026801:Synteny
chemical_list: D012329:RNA, Bacterial; D012335:RNA, Ribosomal