title: Comparative assessment of the intracellular survival of the Burkholderia pseudomallei bopC mutant.
abstract: Burkholderia pseudomallei, the causative agent of melioidosis, is a Gram-negative saprophytic bacterium capable of surviving within phagocytic cells. To assess the role of BopC (a type III secreted effector protein) in the pathogenesis of B. pseudomallei, a B. pseudomallei bopC mutant was used to infect J774A.1 macrophage-like cells. The bopC mutant showed significantly reduced intracellular survival in infected macrophages compared to wild-type B. pseudomallei. In addition, the bopC mutant displayed delayed escape from endocytic vesicles compared with the wild-type strain. This indicates that BopC is important, and at least in part, needed for intracellular survival of B. pseudomallei.
authors.affiliation: Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
authors.forename: Varintip
authors.initials: V
authors.lastname: Srinon
authors.forename: Sunsiree
authors.initials: S
authors.lastname: Muangman
authors.forename: Nithima
authors.initials: N
authors.lastname: Imyaem
authors.forename: Veerachat
authors.initials: V
authors.lastname: Muangsombut
authors.forename: Natalie R
authors.initials: NR
authors.lastname: Lazar Adler
authors.forename: Edouard E
authors.initials: EE
authors.lastname: Galyov
authors.forename: Sunee
authors.initials: S
authors.lastname: Korbsrisate
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D016957:Burkholderia pseudomallei / Q000235:genetics* / Q000378:metabolism*; D052118:Lysosomal-Associated Membrane Protein 1 / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology; D008554:Melioidosis / Q000378:metabolism / Q000382:microbiology; D051379:Mice; D009154:Mutation; D011485:Protein Binding
chemical_list: D000199:Actins; D001426:Bacterial Proteins; D052118:Lysosomal-Associated Membrane Protein 1