title: Mesenchymal stromal cell turnover in the normal adult lung revisited.
abstract: We have employed a simple and robust noninvasive method of continuous in vivo long-term bromodeoxyuridine (BrdU) labeling to analyze lung mesenchymal stromal cell turnover in adult mice in the steady state. Mathematical modeling of BrdU uptake in flow cytometrically sorted CD45(neg)CD31(neg)Sca-1(pos) lung cells following long-term feeding of BrdU to mice in their drinking water reveals that lung mesenchymal stromal cells cycle continuously throughout life. Analysis of BrdU incorporation during long-term feeding and during chasing (delabeling) following replacement of BrdU-water with normal water shows that the CD45(neg)CD31(neg)Sca-1(pos) lung mesenchymal stromal cell compartment turns over at a rate of ∼2.26% per day with a time to half-cycled of 44 days, an estimated cell proliferation rate of 0.004/day, and a cell death rate of 0.018/day.
authors.affiliation: Lung Health Research Centre, Dept. of Pharmacology and Therapeutics, The Univ. of Melbourne, Victoria 3010, Australia. ivanb@unimelb.edu.au.
authors.forename: Yashar
authors.initials: Y
authors.lastname: Seyed-Razavi
authors.forename: Brenda
authors.initials: B
authors.lastname: Williams
authors.forename: David A
authors.initials: DA
authors.lastname: Winkler
authors.forename: Ivan
authors.initials: I
authors.lastname: Bertoncello
mesh_terms: D000367:Age Factors; D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism; D016923:Cell Death / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D005434:Flow Cytometry; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008168:Lung / Q000166:cytology* / Q000502:physiology*; D008565:Membrane Proteins / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D013047:Specific Pathogen-Free Organisms
chemical_list: D000950:Antigens, Ly; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D017493:Leukocyte Common Antigens; C516770:Ptprc protein, mouse
keywords: bromodeoxyuridine; cell cycling; cell turnover; lung mesenchymal stromal cells