title: [Study on chromatography-efficacy relationship of anti-inflammatory activity of qizhi weitong particle compound herbs with neural network and gray correlation method].
abstract: OBJECTIVE
  To establish the chromatography-efficacy relation method for analyzing the anti-inflammatory activity of Qizhi Weitong particles, in order to lay a foundation for quality control and pharmacodynamic evaluation of traditional Chinese medicine compounds.
  
  
  METHODS
  On the basis of a full-time multi-wavelength fusion fingerprint of Qizhi Weitong particles, the latin hypercube sampling was used to divide six herbs in Qizhi Weitong particles into groups of different proportions to determine their inhibition ratios of TNF-alpha, IL-6 and NO released by LPS-induced RAW264. 7 cells. Pharmaeodynamic data and chemical information of HPLC fingerprints of each group were analyzed with the gray correlation method to get the anti-inflammatory effect of each chromatographic peak, and then fitted with BP neural network to establish the chromatography-efficacy relation.
  
  
  RESULTS
  There were 25 peaks closely related to the anti-inflammatory activity. With the 25 peaks as input items, the 3-BP network was adopted to establish the neural network model for anti-inflammatory effect of Qizhi Weitong particles.
  
  
  CONCLUSIONS
  With an error of less than 7%, the model could better fit with the complicated non-linear relation of the compound, and applied in studying the chromatography-efficacy relation. In this study on the chromatography-efficacy relation, a new method is established to evaluate the anti-inflammatory activity of Qizhi Weitong particles. It is of practical significance as an effective approach for controlling quality and exploring the material basis for efficacy of traditional Chinese medicine compounds.
authors.affiliation: College of Pharmacy, Liaoning University of Chinese Medicine, Dalian 116600, China. xww159@sina.com
authors.forename: Wen-Wen
authors.initials: WW
authors.lastname: Xu
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.forename: Xian-Sheng
authors.initials: XS
authors.lastname: Meng
authors.forename: Yong-Rui
authors.initials: YR
authors.lastname: Bao
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000737:chemistry*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry*; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D016571:Neural Networks, Computer; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D013272:Stomach Diseases / Q000188:drug therapy* / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha