title: Study of selenocompounds from selenium-enriched culture of edible sprouts.
abstract: Selenium is recognised as an essential micronutrient for humans and animals. One of the main sources of selenocompounds in the human diet is vegetables. Therefore, this study deals with the Se species present in different edible sprouts grown in Se-enriched media. We grew alfalfa, lentil and soy in a hydroponic system amended with soluble salts, containing the same proportion of Se, in the form of Se(VI) and Se(IV). Total Se in the sprouts was determined by acidic digestion in a microwave system and by ICP/MS. Se speciation was carried out by enzymatic extraction (Protease XIV) and measured by LC-ICP/MS. The study shows that the Se content of plants depends on the content in the growth culture, and that part of the inorganic Se was biotransformed mainly into SeMet. These results contribute to our understanding of the uptake of inorganic Se and its biotransformation by edible plants.
authors.affiliation: Departament de Química Analítica, Facultat de Química, Universitat de Barcelona, C/Martí i Franquès 1-11, E-08028 Barcelona, Spain.
authors.forename: Virginia
authors.initials: V
authors.lastname: Funes-Collado
authors.forename: Albert
authors.initials: A
authors.lastname: Morell-Garcia
authors.forename: Roser
authors.initials: R
authors.lastname: Rubio
authors.forename: José Fermín
authors.initials: JF
authors.lastname: López-Sánchez
mesh_terms: D001711:Biotransformation; D018525:Germination; D018527:Hydroponics / Q000295:instrumentation; D027806:Lens Plant / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D000455:Medicago sativa / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D012639:Seeds / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism; D012643:Selenium / Q000378:metabolism*; D012645:Selenomethionine / Q000032:analysis* / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D014675:Vegetables / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism
chemical_list: D012645:Selenomethionine; D012643:Selenium
keywords: Edible plants; Enzymatic extraction; LC-ICP/MS; Se-amino acids; Selenate; Selenite