title: Using Galleria mellonella-Candida albicans infection model to evaluate antifungal agents.
abstract: Candida albicans is the most common fungal pathogen. Galleria mellonella is widely used as an infection model host. Nevertheless, the G. mellonella-C. albicans infection model had not been optimized for drug evaluation before this study. In this work, we revealed that 5 × 10(5) colony forming unit (CFU)/larva was a suitable inoculum to optimize the G. mellonella-C. albicans infection model in order to evaluate antifungal agents. Using our optimized model, the antifungal effect of fluconazole, amphotericin B and flucytosine, and the synergy between amphotericin B and flucytosine were successfully verified. Thus, this study provides a rapid, inexpensive and reliable way to evaluate antifungals in vivo.
authors.affiliation: New Drug Research and Development Center, School of Pharmacy, Second Military Medical University.
authors.forename: De-Dong
authors.initials: DD
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Deng
authors.forename: Gan-Hai
authors.initials: GH
authors.lastname: Hu
authors.forename: Lan-Xue
authors.initials: LX
authors.lastname: Zhao
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Hu
authors.forename: Yuan-Ying
authors.initials: YY
authors.lastname: Jiang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology; D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000472:pathogenicity; D004195:Disease Models, Animal; D015725:Fluconazole / Q000494:pharmacology; D005437:Flucytosine / Q000494:pharmacology; D007814:Larva / Q000382:microbiology; D009036:Moths / Q000382:microbiology*
chemical_list: D000935:Antifungal Agents; D000666:Amphotericin B; D015725:Fluconazole; D005437:Flucytosine