title: Effect of Brassica oleracea in rats skin wound healing.
abstract: OBJECTIVE
  To investigate the effect of Brassica oleracea herbal balsam on the healing of skin wounds in rats.
  
  
  METHODS
  Twenty four rats (Wistar, 60 days, 250 g) were divided into four groups: untreated animals (C) and treated with the ointment (T), subdivided into two experimental times (seven and 16 days). A 3cm² skin wound was made in the back of all animals. 100 ml of the Brassica oleracea was applied twice a day in T group. Biometric analysis was made with images captured at one, four, seven, ten, 13, and 16 days. At seven and 16 days, animals of each group were euthanized. The wound area removed was processed for histological and histomorphometric analysis to quantify birefringent collagen fibers. Statistical analysis was made considering p < 0.05 as significant.
  
  
  RESULTS
  Biometric analysis revealed no significant differences between groups in both experimental times studied. However, histomorphometric analysis showed that the number of type I collagen fibers was significantly higher in the specimens of the group T16 compared to the other groups.
  
  
  CONCLUSIONS
  Brassica oleracea accelerated the wound healing process increasing the number of type I collagen fibers and the maturity of the newly formed tissue.
authors.affiliation: Biomedical Engineering, University Valley of Paraiba (UNIVAP), Sao Jose dos Campos-SP, Brazil.
authors.forename: Aline
authors.initials: A
authors.lastname: Rebolla
authors.forename: Emilia Angela Lo Schiavo
authors.initials: EA
authors.lastname: Arisawa
authors.forename: Paulo Roxo
authors.initials: PR
authors.lastname: Barja
authors.forename: Maria Belén Salazar
authors.initials: MB
authors.lastname: Posso
authors.forename: Carolina da Silva
authors.initials: Cda S
authors.lastname: Carvalho
mesh_terms: D000818:Animals; D001699:Biometry; D001937:Brassica / Q000737:chemistry*; D003094:Collagen / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012867:Skin / Q000293:injuries*; D013718:Tensile Strength; D013997:Time Factors; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D010936:Plant Extracts; D003094:Collagen