title: Evaluation of the Effects of Caffeic Acid Phenethyl Ester on Prostaglandin E2 and Two Key Cytokines Involved in Bleomycin-induced Pulmonary Fibrosis.
abstract: OBJECTIVE
  Pulmonary fibrosis (PF) is the most common outcome of a collection of diverse lung disorders known as interstitial lung diseases. It is proposed that alterations in the levels of fibrogenic mediators and the profibrotic/antifibrotic imbalance play a substantial role in the progression of PF in animal models and possibly in humans. Caffeic acid phenethyl ester (CAPE), an active component of propolis, has numerous biological effects. In the present study, the main objective was to investigate the effects of CAPE on some key mediators including TGF-β1, TNF-α and prostaglandin E2 (PGE2) involved in profibrotic/antifibrotic balance and pathogenesis of idiopathic pulmonary fibrosis (IPF).
  
  
  METHODS
  In this study, forty male Sprague-Dawley rats were divided into 5 groups (n=8). (1) "Bleomycin (BLM)-treated (Model) group": BLM (5 mg/kg, single intratracheal dose), (2) "Saline-treated group": the rats were given only saline, (3) "Treatment-1 group": BLM + CAPE (5 μmol/kg/day, 28 days, IP), (4) "Treatment-2 group": BLM + CAPE (10 μmol/kg/day, 28 days, IP) and (5) "Vehicle + CAPE group": CAPE (10 μmol/kg/day, 28 days, IP).
  
  
  RESULTS
  BLM could significantly increase the levels of TNF-α and TGF-β1 and decrease the PGE2 concentration compared to the saline control group. CAPE could considerably improve these values almost close to normal levels.
  
  
  CONCLUSIONS
  Briefly, CAPE can be suggested as a novel, attractive and effective agent for prevention and treatment of pulmonary fibrosis.
authors.affiliation: Department of Pharmacology and Toxicology, School of Pharmacy and Physiology Research Center, Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Larki-Harchegani
authors.forename: Ali Asghar
authors.initials: AA
authors.lastname: Hemmati
authors.forename: Ardeshir
authors.initials: A
authors.lastname: Arzi
authors.forename: Mehri
authors.initials: M
authors.lastname: Ghafurian-Boroojerdnia
authors.forename: Somayeh
authors.initials: S
authors.lastname: Shabib
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Zadkarami
authors.forename: Saleh
authors.initials: S
authors.lastname: Esmaeilzadeh
keywords: Bleomycin; CAPE; Cytokine; PGE2; Pulmonary Fibrosis; TGF-β1; TNF-α