title: Ascorbic acid and BSA protein in solution and films: interaction and surface morphological structure.
abstract: This paper reports on the study of the interactions between ascorbic acid (AA) and bovine serum albumin (BSA) in aqueous solution as well as in films (BSA/AA films) prepared by the layer-by-layer technique. Regarding to solution studies, a hyperchromism (in the range of ultraviolet) was found as a function of AA concentration, which suggested the formation of aggregates from AA and BSA. Binding constant, K, determined for aggregates from BSA and AA was found to be about 10(2) M(-1), which indicated low affinity of AA with BSA. For the BSA/AA films, it was also noted that the AA adsorption process and surface morphological structures depended on AA concentration. By changing the contact time between the AA and BSA, a hypochromism was revealed, which was associated to decrease of accessibility of solvent to tryptophan due to formation of aggregates. Furthermore, different morphological structures of aggregates were observed, which were attributed to the diffusion-limited aggregation. Since most of studies of interactions of drugs and proteins are performed in solution, the analysis of these processes by using films can be very valuable because this kind of system is able to employ several techniques of investigation in solid state.
authors.affiliation: Grupo de Materiais Nanoestruturados, Campus Universitário do Araguaia, Universidade Federal de Mato Grosso, 78600-000 Barra do Garças, MT, Brazil.
authors.forename: Rafael R G
authors.initials: RR
authors.lastname: Maciel
authors.forename: Adriele A
authors.initials: AA
authors.lastname: de Almeida
authors.forename: Odin G C
authors.initials: OG
authors.lastname: Godinho
authors.forename: Filipe D S
authors.initials: FD
authors.lastname: Gorza
authors.forename: Graciela C
authors.initials: GC
authors.lastname: Pedro
authors.forename: Tarquin F
authors.initials: TF
authors.lastname: Trescher
authors.forename: Josmary R
authors.initials: JR
authors.lastname: Silva
authors.forename: Nara C
authors.initials: NC
authors.lastname: de Souza
mesh_terms: D000042:Absorption; D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry* / Q000378:metabolism*; D002417:Cattle; D016014:Linear Models; D018625:Microscopy, Atomic Force; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism*; D012996:Solutions; D013997:Time Factors
chemical_list: D012996:Solutions; D012710:Serum Albumin, Bovine; D001205:Ascorbic Acid