title: Structure of the Aeropyrum pernix L7Ae multifunctional protein and insight into its extreme thermostability.
abstract: Archaeal ribosomal protein L7Ae is a multifunctional RNA-binding protein that directs post-transcriptional modification of archaeal RNAs. The L7Ae protein from Aeropyrum pernix (Ap L7Ae), a member of the Crenarchaea, was found to have an extremely high melting temperature (>383 K). The crystal structure of Ap L7Ae has been determined to a resolution of 1.56 Å. The structure of Ap L7Ae was compared with the structures of two homologs: hyperthermophilic Methanocaldococcus jannaschii L7Ae and the mesophilic counterpart mammalian 15.5 kD protein. The primary stabilizing feature in the Ap L7Ae protein appears to be the large number of ion pairs and extensive ion-pair network that connects secondary-structural elements. To our knowledge, Ap L7Ae is among the most thermostable single-domain monomeric proteins presently observed.
authors.affiliation: Conagen Inc., Saint Louis, MO 63132, USA. wadud@conagen-inc.com
authors.forename: Mohammad Wadud
authors.initials: MW
authors.lastname: Bhuiya
authors.forename: Jimmy
authors.initials: J
authors.lastname: Suryadi
authors.forename: Zholi
authors.initials: Z
authors.lastname: Zhou
authors.forename: Bernard Andrew
authors.initials: BA
authors.lastname: Brown
mesh_terms: D041522:Aeropyrum / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics; D001665:Binding Sites; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics; D006358:Hot Temperature; D006801:Humans; D006860:Hydrogen Bonding; D007477:Ions / Q000737:chemistry*; D063747:Methanocaldococcus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D055550:Protein Stability; D017433:Protein Structure, Secondary; D019642:RNA, Archaeal / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment; D040681:Structural Homology, Protein
chemical_list: D019843:Archaeal Proteins; D007477:Ions; D019642:RNA, Archaeal; D011994:Recombinant Proteins; D012269:Ribosomal Proteins
keywords: Aeropyrum pernix; L7Ae; ion pairs; ribosomal proteins; thermal stability