title: The effect of a commercial probiotic drink on oral microbiota in healthy complete denture wearers.
abstract: BACKGROUND
  It is acknowledged that oral and general health status declines with age. The global population of denture wearers is increasing, so is the incidence of denture biofilm-related problems, such as denture-associated stomatitis, aspiration pneumonia and malodour. It has been suggested that consumption of probiotic bacteria may improve oral health. However, the effects of probiotics on the oral microbiota of denture wearers have received little attention.
  
  
  METHODS
  The aim of this study was to investigate the effect of consumption of a commercial probiotic product (Yakult) on microbiota of saliva, tongue and denture biofilm in healthy complete denture wearers. Eight healthy complete denture-wearing National Health Service (NHS) patients undertook a 7-week trial comprising three phases: baseline; 4-week consumption of one bottle of Yakult per day, each containing a minimum 6.5×10(9) viable cells of Lactobacillus casei strain Shirota (LcS); 4-week washout period. The microbial viability and composition of saliva, tongue dorsum coating and denture biofilm were assessed using a range of solid selective and indicator media. Questionnaires were used to explore participants' denture cleaning habits and impact of wearing dentures on their life quality and well-being [modified oral health impact profile (OHIP-14)] prior to and after the study.
  
  
  RESULTS
  Seven volunteers (1 male, 6 females) completed the trial. LcS temporarily colonised oral cavity and denture surface. There was no significant change in the viability of Streptococcus mutans, acidogenic microorganisms, total anaerobic species and Gram-negative obligate anaerobes between study phases. There was no obvious effect of LcS on occurrence and viability of Candida. Participants presented a good general knowledge of denture hygiene and their responses to OHIP-14 questionnaires improved after completing the study (p=0.16).
  
  
  CONCLUSIONS
  It appeared that 4-week consumption of probiotic drink had no overall effect on selected oral parameters in healthy denture wearers despite temporary presence of LcS.
authors.affiliation: Microbiology Research Group, School of Health Care Science, Manchester Metropolitan University, Manchester, UK.
authors.forename: Justyna
authors.initials: J
authors.lastname: Sutula
authors.forename: Lisa
authors.initials: L
authors.lastname: Coulthwaite
authors.forename: Linda
authors.initials: L
authors.lastname: Thomas
authors.forename: Joanna
authors.initials: J
authors.lastname: Verran
keywords: Candida; Lactobacillus casei Shirota; OHIP; colonisation; denture biofilm; denture wearers; saliva; well-being