title: [Salidroside inhibits hypoxia-induced phenotypic modulation of corpus cavernosum smooth muscle cells in vitro].
abstract: OBJECTIVE
  To explore the effects of salidroside on the phenotypic modulation of corpus cavernosum smooth muscle cells (CCSMC) in hypoxic SD rats.
  
  
  METHODS
  CCSMCs were cultured in vitro and identified by immunohistochemistry. The cells were divided into six groups: normal control (21% O2), hypoxia (1% O2), hypoxia + salidroside 1 mg/L, hypoxia + salidroside 3 mg/L, hypoxia + salidroside 5 mg/L and hypoxia + PGE1 0.4 microg/L, and then cultured for 48 hours. The relative expressions of alpha-actin and osteopontin (OPN) in each group were determined by RT-PCR.
  
  
  RESULTS
  The in vitro cultured CCSMCs grew well, with anti-alpha-smooth muscle actin monoclonal antibodies immunohistochemically positive. The relative expression of alpha-actin was markedly decreased while that of OPN remarkably increased in the hypoxia group as compared with the normal control group (P < 0.01). The hypoxia + salidroside 5 mg/L group showed a significantly higher expression of alpha-actin and lower expression of OPN than the hypoxia group (P < 0.01), but exhibited no significant differences from the hypoxia + PGE group (P > 0.05).
  
  
  CONCLUSIONS
  Hypoxia can reduce the relative expression level of alpha-actin and increase that of OPN in the CCSMCs of SD rats, namely, induce their phenotypic modulation from the contraction to the non-contraction type. Salidroside can restrain hypoxia-induced phenotypic modulation of CCSMCs, and its inhibitory effect at 5 mg/L is similar to that of PGE1.
authors.affiliation: Department of Urology, The Second Hospital Affiliated to Zhejiang University of Chinese Medicine, Hangzhou, Zhejiang 310005, China. chengang0911@163.com
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Huang
authors.forename: Bo-Dong
authors.initials: BD
authors.lastname: Lü
authors.forename: Shi-Tao
authors.initials: ST
authors.lastname: Chen
authors.forename: Shi-Geng
authors.initials: SG
authors.lastname: Zhang
authors.forename: Ke-Bing
authors.initials: KB
authors.lastname: Yang
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D005960:Glucosides / Q000494:pharmacology*; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000378:metabolism*; D053495:Osteopontin / Q000378:metabolism; D010413:Penis / Q000166:cytology; D010636:Phenols / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000199:Actins; D005960:Glucosides; D053495:Osteopontin; D010636:Phenols; C506897:Spp1 protein, rat; C498862:Acta2 protein, rat; C009172:rhodioloside