title: Bacterial community diversity in a low-permeability oil reservoir and its potential for enhancing oil recovery.
abstract: The diversity of indigenous bacterial community and the functional species in the water samples from three production wells of a low permeability oil reservoir was investigated by high-throughput sequencing technology. The potential of application of indigenous bacteria for enhancing oil recovery was evaluated by examination of the effect of bacterial stimulation on the formation water-oil-rock surface interactions and micromodel test. The results showed that production well 88-122 had the most diverse bacterial community and functional species. The broth of indigenous bacteria stimulated by an organic nutrient activator at aerobic condition changed the wettability of the rock surface from oil-wet to water-wet. Micromodel test results showed that flooding using stimulated indigenous bacteria following water flooding improved oil recovery by 6.9% and 7.7% in fractured and unfractured micromodels, respectively. Therefore, the zone of low permeability reservoir has a great potential for indigenous microbial enhanced oil recovery.
authors.affiliation: State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing 102249, PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing 102249, PR China. Electronic address: bjzzzhang@163.com.
authors.forename: Zhong-Zhi
authors.initials: ZZ
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing 102249, PR China.
authors.forename: Jing-Xiu
authors.initials: JX
authors.lastname: Wang
authors.affiliation: School of Mechanical, Materials and Mechatronic Engineering, University of Wollongng, Wollongong, NSW 2522, Australia.
authors.forename: Guang-Qing
authors.initials: GQ
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing 102249, PR China.
authors.forename: Yi-Jing
authors.initials: YJ
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing 102249, PR China.
authors.forename: Zhao-Zheng
authors.initials: ZZ
authors.lastname: Song
authors.affiliation: Exploration and Development Research Institution, Daqing Oilfield Company Ltd., Daqing, Heilongjiang 163712, PR China.
authors.forename: Ji-Yuan
authors.initials: JY
authors.lastname: Zhang
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D044822:Biodiversity; D009821:Oils / Q000378:metabolism*; D010539:Permeability; D013500:Surface Tension
chemical_list: D009821:Oils
keywords: Bacterial community diversity; Enhanced oil recovery; Glass micromodel; High-throughput sequencing; Wettability