title: Kinetics of enzyme-catalyzed hydrolysis of steam-exploded sugarcane bagasse.
abstract: This work presents the experimental kinetic data and the fractal modeling of sugarcane bagasse steam treatment and enzymatic hydrolysis. Sugarcane bagasse (50 wt% moisture) was pretreated by autohydrolysis at 210 °C for 4 min. Acid catalysis involved the use of 9.5mg g(-1) of H2SO4 or H3PO4 in relation to the substrate dry mass at these same pretreatment conditions. Unwashed, water-washed and alkali-washed substrates were hydrolyzed at 2.0 wt% using 8 and 15 FPU g(-1) (108.22 and 199.54 mg/g) total solids of a Celluclast 1.5 L and Novozym 188 mixture (Novozymes). The fractal kinetic modeling was used to describe the effect of pretreatment and both washing processes on substrate accessibility. Water and/or alkali washing was not strictly necessary to achieve high hydrolysis efficiencies. Also, the fractal model coefficients revealed that H3PO4 was a better pretreatment catalyst under the experimental conditions used in this study, resulting in the most susceptible substrates for enzymatic hydrolysis.
authors.affiliation: Research Center in Applied Chemistry (CEPESQ), Department of Chemistry, Federal University of Paraná (UFPR), Curitiba PR 81531-990, Brazil; Department of Chemical Engineering, Federal University of Paraná (UFPR), Curitiba, PR 82530-990, Brazil.
authors.forename: Rodrigo Souza
authors.initials: RS
authors.lastname: Aguiar
authors.affiliation: Research Center in Applied Chemistry (CEPESQ), Department of Chemistry, Federal University of Paraná (UFPR), Curitiba PR 81531-990, Brazil.
authors.forename: Marcos Henrique Luciano
authors.initials: MHL
authors.lastname: Silveira
authors.affiliation: Research Center in Applied Chemistry (CEPESQ), Department of Chemistry, Federal University of Paraná (UFPR), Curitiba PR 81531-990, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Pitarelo
authors.affiliation: Department of Chemical Engineering, Federal University of Paraná (UFPR), Curitiba, PR 82530-990, Brazil.
authors.forename: Marcos Lucio
authors.initials: ML
authors.lastname: Corazza
authors.affiliation: Research Center in Applied Chemistry (CEPESQ), Department of Chemistry, Federal University of Paraná (UFPR), Curitiba PR 81531-990, Brazil. Electronic address: luiz.ramos@ufpr.br.
authors.forename: Luiz Pereira
authors.initials: LP
authors.lastname: Ramos
mesh_terms: D002384:Catalysis; D005740:Gases / Q000737:chemistry*; D007700:Kinetics; D031786:Saccharum / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D005740:Gases; D014867:Water
keywords: Enzymatic hydrolysis; Fractal kinetics; Steam explosion; Sugarcane bagasse