title: Effects of polysaccharide isolated from Streptococcus thermophilus CRL1190 on human gastric epithelial cells.
abstract: EPS1190 was isolated from skim milk fermented with Stretococcus thermophilus CRL1190. The polysaccharide consisted of 33% glucose and 66% galactose with 1,4- and 1,4,6-galactose residues as main building blocks beside a high amount of 1,4-linked glucose. The polymer was characterized additionally concerning viscosity and zeta potential. EPS1190 stimulated cellular vitality and proliferation of human stomach AGS cells and human buccal KB cells significantly. EPS1190 stimulated phagocytosis rate of murine macrophages RAW264.7 significantly. NO-release or anti-inflammatory effects by inhibition of LPS-induced NO release were not observed. Confocal laser scanning microscopy revealed that EPS1190 is partially internalized into AGS cells via endosomes. The bioadhesive absorption of FITC-labeled EPS1190 into the mucus layer on the apical side of the epithelium using histological tissue sections from human stomach was observed. Specific interaction of EPS1190 with mucin can be excluded as shown by microviscosimetry studies. EPS1190 increased the adhesion of H. pylori to AGS cells, which resulted in increased secretion of proinflammatory cytokines TNFa, IL-6 and IL-8. Summarizing, EPS1190 seems to stimulate epithelial cell regeneration and immunological innate defense mechanisms, which again can rationalized the use of this polysaccharide as cytoprotective compound in probiotioc preparations.
authors.affiliation: University of Münster, Institute for Pharmaceutical Biology and Phytochemistry (IPBP), Corrensstrasse 48, D-48149 Münster, Germany; Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, San Miguel de Tucumán 4000, Tucumán, Argentina.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Marcial
authors.forename: Jutta
authors.initials: J
authors.lastname: Messing
authors.forename: Bianca
authors.initials: B
authors.lastname: Menchicchi
authors.forename: Francisco M
authors.initials: FM
authors.lastname: Goycoolea
authors.forename: Gerhard
authors.initials: G
authors.lastname: Faller
authors.forename: Font de Valdez
authors.initials: Fde V
authors.lastname: Graciela
authors.forename: Andreas
authors.initials: A
authors.lastname: Hensel
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects* / Q000382:microbiology; D016998:Helicobacter / Q000502:physiology; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D011134:Polysaccharides / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology*; D013270:Stomach / Q000166:cytology*; D048249:Streptococcus thermophilus / Q000378:metabolism*
chemical_list: D016207:Cytokines; D011134:Polysaccharides
keywords: CLS; Cytoprotection; EPS; Exo-polysaccharide; FITC; Helicobacter pylori; Human gastric epithelial cells; MOI; Mucin; Streptococcus thermophilus; confocal laser scanning microscopy; exo-polysaccharide; fluorescein isoisothiocyanate; multiplicity of infection