title: Correlation between the activity of digestive enzymes and nonself recognition in the gut of Eisenia andrei earthworms.
abstract: Earthworms Eisenia andrei, similarly to other invertebrates, rely on innate defense mechanisms based on the capability to recognize and respond to nonself. Here, we show a correlation between the expression of CCF, a crucial pattern-recognition receptor, and lysozyme, with enzyme activities in the gut of E. andrei earthworms following a microbial challenge. These data suggest that enzyme activities important for the release and recognition of molecular patterns by pattern-recognition molecules, as well as enzymes involved in effector pathways, are modulated during the microbial challenge. In particular, protease, laminarinase, and glucosaminidase activities were increased in parallel to up-regulated CCF and lysozyme expression.
authors.affiliation: Institute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic.
authors.forename: Petra
authors.initials: P
authors.lastname: Procházková
authors.forename: Vladimír
authors.initials: V
authors.lastname: Šustr
authors.forename: Jiří
authors.initials: J
authors.lastname: Dvořák
authors.forename: Radka
authors.initials: R
authors.lastname: Roubalová
authors.forename: František
authors.initials: F
authors.lastname: Škanta
authors.forename: Václav
authors.initials: V
authors.lastname: Pižl
authors.forename: Martin
authors.initials: M
authors.lastname: Bilej
mesh_terms: D000818:Animals; D001412:Bacillus subtilis / Q000276:immunology; D044602:Cellulases / Q000378:metabolism; D004926:Escherichia coli / Q000276:immunology; D006596:Hexosaminidases / Q000378:metabolism; D007113:Immunity, Innate; D009113:Muramidase / Q000378:metabolism / Q000502:physiology*; D009835:Oligochaeta / Q000201:enzymology* / Q000276:immunology / Q000382:microbiology; D010447:Peptide Hydrolases / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000276:immunology; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; D044602:Cellulases; D006596:Hexosaminidases; D009113:Muramidase; D010447:Peptide Hydrolases
keywords: Digestive enzyme; Earthworm; Eisenia andrei; Gut; Innate immunity; Microbiota