title: Designing experiments to understand the variability in biochemical reaction networks.
abstract: Exploiting the information provided by the molecular noise of a biological process has proved to be valuable in extracting knowledge about the underlying kinetic parameters and sources of variability from single-cell measurements. However, quantifying this additional information a priori, to decide whether a single-cell experiment might be beneficial, is currently only possible in systems where either the chemical master equation is computationally tractable or a Gaussian approximation is appropriate. Here, we provide formulae for computing the information provided by measured means and variances from the first four moments and the parameter derivatives of the first two moments of the underlying process. For stochastic kinetic models for which these moments can be either computed exactly or approximated efficiently, the derived formulae can be used to approximate the information provided by single-cell distribution experiments. Based on this result, we propose an optimal experimental design framework which we employ to compare the utility of dual-reporter and perturbation experiments for quantifying the different noise sources in a simple model of gene expression. Subsequently, we compare the information content of a set of experiments which have been performed in an engineered light-switch gene expression system in yeast and show that well-chosen gene induction patterns may allow one to identify features of the system which remain hidden in unplanned experiments.
authors.affiliation: Automatic Control Laboratory, ETH Zurich, 8092 Zurich, Switzerland.
authors.forename: Jakob
authors.initials: J
authors.lastname: Ruess
authors.forename: Andreas
authors.initials: A
authors.lastname: Milias-Argeitis
authors.forename: John
authors.initials: J
authors.lastname: Lygeros
mesh_terms: D003198:Computer Simulation; D008954:Models, Biological; D012107:Research Design; D013269:Stochastic Processes
keywords: Fisher information; cell-to-cell variability; continuous-time Markov chains; gene expression; optimal experimental design