title: Diamine oxidase as a marker of intestinal mucosal injury and the effect of soluble dietary fiber on gastrointestinal tract toxicity after intravenous 5-fluorouracil treatment in rats.
abstract: The level of plasma diamine oxidase (DAO) activity is associated with the maturation and integrity of small intestinal mucosa. This study in rats investigated whether a decreased level of plasma DAO could reflect the severity of mucosal injury due to intravenous 5-fluorouracil (5-FU) treatment. The beneficial effect of soluble dietary fiber (SDF) on preventing diarrhea after 5-FU treatment was also examined. To induce diarrhea, 5-FU (50 mg/kg/day for four days) was administered via the tail vein with or without SDF supplementation. After 5-FU treatment, the majority of rats developed moderate to severe diarrhea, and levels of plasma DAO activity significantly decreased compared to those of control group (P < 0.05). Scanning electron microscopy revealed disarrangement of the small intestinal villi. Contrarily, the rats supplemented with SDF had diarrhea less frequently (50.0 vs. 91.7 %, P = 0.025) on day five, and DAO activity levels were significantly higher than in those rats administered 5-FU alone (8.25 ± 5.34 vs. 5.50 ± 4.32, P = 0.023). In conclusion, plasma DAO activity decreases in response to severe intestinal mucosal injury after 5-FU treatment, and SDF supplementation might be a practical and useful treatment for reducing the intestinal toxicity of 5-FU.
authors.affiliation: Department of Surgery, Kochi Medical School, Kochi, Japan.
authors.forename: Ian
authors.initials: I
authors.lastname: Fukudome
authors.forename: Michiya
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Ken
authors.initials: K
authors.lastname: Dabanaka
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Maeda
authors.forename: Ken
authors.initials: K
authors.lastname: Okamoto
authors.forename: Takehiro
authors.initials: T
authors.lastname: Okabayashi
authors.forename: Ryoko
authors.initials: R
authors.lastname: Baba
authors.forename: Nana
authors.initials: N
authors.lastname: Kumagai
authors.forename: Koji
authors.initials: K
authors.lastname: Oba
authors.forename: Mamoru
authors.initials: M
authors.lastname: Fujita
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Hanazaki
mesh_terms: D061605:Administration, Intravenous; D006631:Amine Oxidase (Copper-Containing) / Q000097:blood*; D000818:Animals; D015415:Biomarkers / Q000097:blood*; D003967:Diarrhea / Q000139:chemically induced / Q000517:prevention & control*; D004043:Dietary Fiber / Q000494:pharmacology*; D005472:Fluorouracil / Q000008:administration & dosage / Q000633:toxicity*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000187:drug effects / Q000293:injuries* / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D051381:Rats; D018709:Statistics, Nonparametric
chemical_list: D015415:Biomarkers; D004043:Dietary Fiber; D006631:Amine Oxidase (Copper-Containing); D005472:Fluorouracil