title: The role of potassium channel in silica nanoparticle-induced inflammatory effect in human vascular endothelial cells in vitro.
abstract: Exposure to nanoparticles became popular in industry and daily life. Nano-SiO₂ was shown to have an adverse effect to vascular endothelial cell although the mechanisms remain unclear. To test whether the nano-SiO₂'s harmful effect was related to the potassium channel, human umbilical vascular endothelial cells (HUVECs) were treated with nano-SiO₂ in different dose. Cell survival rate and lactate dehydrogenase (LDH) as cytotoxic parameters, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) as inflammation indicators were determined. The electrophysiological changes and function of potassium channel were detected with patch clamp and channel blockers. It was found that nano-SiO₂ exposure decreased cell survival rate, increased LDH leakage, TNF-α and IL-6 production. The potassium channel activity was increased in the opening rate and current intensity. Furthermore, potassium channel blockers tetraethylammonium (TEA), 4-amino pyridine (4-AP), and margatoxin (MGTX) reduced the nano-SiO₂-induced cytotoxity and inflammation, i.e., increase in the cell survival rate, and decrease in the LDH leakage and production of TNF-α and IL-6. It might be concluded that the nano-SiO₂-induced inflammation and cytotoxicity at HUVECs was associated with the activation of potassium channel.
authors.affiliation: Department of Occupational Health and MOE Key Lab of Environmental and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Qinqian
authors.initials: Q
authors.lastname: Yan
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Xuecong
authors.initials: X
authors.lastname: Zong
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.forename: Zhenglun
authors.initials: Z
authors.lastname: Wang
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000633:toxicity*; D018408:Patch-Clamp Techniques; D026902:Potassium Channel Blockers / Q000494:pharmacology; D015221:Potassium Channels / Q000378:metabolism*; D012822:Silicon Dioxide / Q000737:chemistry / Q000633:toxicity*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014657:Vasculitis / Q000139:chemically induced* / Q000378:metabolism*
chemical_list: D015850:Interleukin-6; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D014409:Tumor Necrosis Factor-alpha; D012822:Silicon Dioxide; D007770:L-Lactate Dehydrogenase
keywords: Human vascular endothelial cells; Inflammation; Patch clamp; Potassium channel; Silica nanoparticle