title: MicroRNA-124 reduces caveolar density by targeting caveolin-1 in porcine kidney epithelial PK15 cells.
abstract: Caveolin-1 is the principal component of caveolae, and it is implicated in endocytosis, cholesterol homeostasis, signal transduction and tumorigenesis. MicroRNAs play key regulatory roles in many cellular processes. However, the molecular mechanism by which porcine caveolin-1 is regulated by microRNAs remains unclear. In the present study, we found that miR-124 could directly target caveolin-1 in porcine kidney epithelial cells (PK15). A luciferase reporter assay revealed that miR-124 directly bound to Cav1 mRNA. Ectopic expression of miR-124 decreased porcine Cav1 expression at both the mRNA and protein levels. Furthermore, we used transmission electron microscopy to count caveolae in the cytosolic space next to the membrane and we found that the overexpression of miR-124 in PK15 cells reduced the density of the caveolae. Our results suggested that miR-124 reduced caveolar density by targeting caveolin-1 in PK15 cells; therefore, miR-124 could play an important role in the caveolae-mediated endocytosis of pathogens and signal transduction.
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education & College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China, a1688816@163.com.
authors.forename: Songbai
authors.initials: S
authors.lastname: Yang
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Liu
authors.forename: Xinyun
authors.initials: X
authors.lastname: Li
authors.forename: Shufeng
authors.initials: S
authors.lastname: Sun
authors.forename: Fei
authors.initials: F
authors.lastname: Sun
authors.forename: Bin
authors.initials: B
authors.lastname: Fan
authors.forename: Shuhong
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000818:Animals; D021941:Caveolae / Q000378:metabolism*; D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism*; D006224:Cricetinae; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D013552:Swine
chemical_list: D051242:Caveolin 1; D035683:MicroRNAs; D012333:RNA, Messenger; D016601:RNA-Binding Proteins