title: Outer membrane proteins ail and OmpF of Yersinia pestis are involved in the adsorption of T7-related bacteriophage Yep-phi.
abstract: Yep-phi is a T7-related bacteriophage specific to Yersinia pestis, and it is routinely used in the identification of Y. pestis in China. Yep-phi infects Y. pestis grown at both 20°C and 37°C. It is inactive in other Yersinia species irrespective of the growth temperature. Based on phage adsorption, phage plaque formation, affinity chromatography, and Western blot assays, the outer membrane proteins of Y. pestis Ail and OmpF were identified to be involved, in addition to the rough lipopolysaccharide, in the adsorption of Yep-phi. The phage tail fiber protein specifically interacts with Ail and OmpF proteins, and residues 518N, 519N, and 523S of the phage tail fiber protein are essential for the interaction with OmpF, whereas residues 518N, 519N, 522C, and 523S are essential for the interaction with Ail. This is the first report to demonstrate that membrane-bound proteins are involved in the adsorption of a T7-related bacteriophage. The observations highlight the importance of the tail fiber protein in the evolution and function of various complex phage systems and provide insights into phage-bacterium interactions.
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
authors.forename: Xiangna
authors.initials: X
authors.lastname: Zhao
authors.forename: Yujun
authors.initials: Y
authors.lastname: Cui
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Yan
authors.forename: Zongmin
authors.initials: Z
authors.lastname: Du
authors.forename: Yafang
authors.initials: Y
authors.lastname: Tan
authors.forename: Huiying
authors.initials: H
authors.lastname: Yang
authors.forename: Yujing
authors.initials: Y
authors.lastname: Bi
authors.forename: Pingping
authors.initials: P
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
authors.forename: Yanping
authors.initials: Y
authors.lastname: Han
authors.forename: Yajun
authors.initials: Y
authors.lastname: Song
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Wang
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D017123:Bacteriophage T7 / Q000378:metabolism*; D015153:Blotting, Western; D002681:China; D002846:Chromatography, Affinity; D054884:Host-Pathogen Interactions; D008070:Lipopolysaccharides / Q000378:metabolism; D008969:Molecular Sequence Data; D010930:Plague / Q000378:metabolism / Q000382:microbiology*; D018272:Porins / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D037521:Virulence Factors / Q000378:metabolism*; D015010:Yersinia pestis / Q000378:metabolism* / Q000472:pathogenicity
chemical_list: C526543:Ail protein, Yersinia pestis; D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides; C109237:OmpF protein; D018272:Porins; D037521:Virulence Factors