title: Murine nonvolatile pheromones: isolation of exocrine-gland secreting Peptide 1.
abstract: Our search for a substance recognized by the vomeronasal neurons revealed that the extra-orbital lacrimal gland (ELG) isolated from adult male mice produced the male-specific peptide pheromone exocrine gland-secreting peptide 1 (ESP1). The following protocol reveals how ESP1 may be extracted from the ELG, purified using anion-exchange and reverse-phase high-performance liquid chromatography (HPLC), and analyzed by mass spectrometry. This protocol has been specifically designed for the purification of ESP1, but may be modified to isolate a variety of peptides from the exocrine glands. Peptides purified in this manner may help further define the molecular mechanisms underlying pheromone communication in the vomeronasal system.
authors.affiliation: Biological Research Laboratories, R&D Division, Daiichi Sankyo Co., LTD., Tokyo, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kimoto
authors.forename: Kazushige
authors.initials: K
authors.lastname: Touhara
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D036341:Intercellular Signaling Peptides and Proteins; D007765:Lacrimal Apparatus / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D010675:Pheromones / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D019147:Vomeronasal Organ / Q000378:metabolism
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D010675:Pheromones; D011506:Proteins; C525947:exocrine-gland-secreting peptide 1, mouse