title: [Structure and immunomodulation activity of a novel mannose binding lectin from housefly pupae].
abstract: We purified a novel mannose binding lectin form Musca domestica pupae by affinity chromatography on Con A-Sepharose 4B and DEAE weak anion-exchange chromatography. By SDS-PAGE, MBL-1 yielded a single band with the molecular weight of 24 kDa. It was a glycoprotein detected by periodic acid-schiffs staining reaction, with 97.36% protein and 2.1% oligosaccharide. Meanwhile, the results of beta-elimination reaction, infrared spectroscopy, atomic force microscopy and protein sequencing instrument show that MBL-1 was an ellipsoidal-shaped monomer with 60-100 nm in diameter. N-glycoside bond linked oligosaccharide chain and the N-terminal blocked peptide chain. Further study suggested that MBL-1 promote the proliferation of macrophage in a concentration-dependent manner. The scanning electron microscope analysis shows that MBL-1 promoted the activation of macrophages. These results show that MBL-1 purified from Musca domestica pupae possesses immune regulation effect, serving a reference basis to develop natural immune-modulator.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, College of Food Engineering and Biotechnology, Tianjin University of Science Technology, Ministry of Education, Tianjin 300457, China. wangchunling@tust.edu.cn
authors.forename: Chunling
authors.initials: C
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Xia
authors.forename: Shijiao
authors.initials: S
authors.lastname: Zhang
authors.forename: Lirui
authors.initials: L
authors.lastname: Wang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Cao
mesh_terms: D000818:Animals; D006023:Glycoproteins / Q000032:analysis; D006793:Houseflies / Q000737:chemistry*; D056747:Immunomodulation / Q000276:immunology* / Q000502:physiology; D008264:Macrophages / Q000276:immunology; D037601:Mannose-Binding Lectin / Q000737:chemistry* / Q000502:physiology*; D009844:Oligosaccharides / Q000032:analysis; D011679:Pupa / Q000737:chemistry
chemical_list: D006023:Glycoproteins; D037601:Mannose-Binding Lectin; D009844:Oligosaccharides