title: Kinetics of enzymatic degradation of high molecular weight polysaccharides through a nanopore: experiments and data-modeling.
abstract: The enzymatic degradation of long polysaccharide chains is monitored by nanopore detection. It follows a Michaelis-Menten mechanism. We measure the corresponding kinetic constants at the single molecule level. The simulation results of the degradation process allowed one to account for the oligosaccharide size distribution detected by a nanopore.
authors.affiliation: Université d'Évry Val d'Essonne , Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement, F-91025 Evry, France.
authors.forename: Aziz
authors.initials: A
authors.lastname: Fennouri
authors.forename: Régis
authors.initials: R
authors.lastname: Daniel
authors.forename: Manuela
authors.initials: M
authors.lastname: Pastoriza-Gallego
authors.forename: Loïc
authors.initials: L
authors.lastname: Auvray
authors.forename: Juan
authors.initials: J
authors.lastname: Pelta
authors.forename: Laurent
authors.initials: L
authors.lastname: Bacri
mesh_terms: D000818:Animals; D002417:Cattle; D006821:Hyaluronoglucosaminidase / Q000493:pharmacokinetics*; D007700:Kinetics; D008297:Male; D008970:Molecular Weight; D058608:Nanopores; D036103:Nanotechnology / Q000379:methods*; D011134:Polysaccharides / Q000493:pharmacokinetics*
chemical_list: D011134:Polysaccharides; D006821:Hyaluronoglucosaminidase