title: Absence of high molecular weight proteins 1 and/or 2 is associated with decreased adherence among non-typeable Haemophilus influenzae clinical isolates.
abstract: High molecular weight (Hmw) proteins 1 and 2, type IV pilin protein (PilA), outer-membrane protein P5 (OmpP5), Haemophilus protein D (Hpd) and Haemophilus adhesive protein (Hap) are surface proteins involved in the adherence of non-typeable Haemophilus influenzae. One hundred clinical isolates were evaluated for the presence of the genes encoding these proteins by PCR and for their adherence capacity (AC) to Detroit 562 nasopharyngeal cells (D562). The majority of isolates were from blood (77/100); other sites were also represented. Confluent D562 monolayers (1.2×10(5) cells per well) were inoculated with standardized minimal infective doses (m.o.i.) of 10(2), 10(3) or 10(4) c.f.u. per well. The AC was categorized as low (<10 %) or high (≥10 %) depending on the percentage of c.f.u. adhering per well. All the isolates evaluated showed adherence: 69/100 (69 %) demonstrated high adherence, while 31/100 (31 %) showed low adherence. Of all the genes evaluated, hmw1A and/or hmw2A were detected in 69/100 (69 %) of isolates. The presence of hmw1A and/or hmw2A was associated with increased adherence to D562 cells (P≤0.001). Dot immunoblots were performed to detect protein expression using mAbs 3D6, AD6 and 10C5. Among the high-adherence isolates (n = 69), 72 % reacted with 3D6 and 21 % with 10C5. Our data indicate that the absence of Hmw1 and/or Hmw2 was associated with decreased adherence to D562 cells.
authors.affiliation: Meningitis and Vaccine Preventable Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: Jeni
authors.initials: J
authors.lastname: Vuong
authors.affiliation: Meningitis and Vaccine Preventable Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Meningitis and Vaccine Preventable Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: Jordan M
authors.initials: JM
authors.lastname: Theodore
authors.affiliation: Meningitis and Vaccine Preventable Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Whitmon
authors.affiliation: Departamento de Salud Pública, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Patricia
authors.initials: P
authors.lastname: Gomez de Leon
authors.affiliation: Meningitis and Vaccine Preventable Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: Leonard W
authors.initials: LW
authors.lastname: Mayer
authors.affiliation: Meningitis and Vaccine Preventable Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: George M
authors.initials: GM
authors.lastname: Carlone
authors.affiliation: Meningitis and Vaccine Preventable Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: Sandra
authors.initials: S
authors.lastname: Romero-Steiner
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D001422:Bacterial Adhesion; D004847:Epithelial Cells / Q000382:microbiology; D006192:Haemophilus Infections / Q000382:microbiology; D006193:Haemophilus influenzae / Q000235:genetics / Q000502:physiology*; D006801:Humans; D016133:Polymerase Chain Reaction
chemical_list: D018829:Adhesins, Bacterial; C117431:HMW1 protein, bacteria; C117432:HMW2 protein, bacteria