title: Wheat gene TaS3 contributes to powdery mildew susceptibility.
abstract: Identification of TaS3 as a potential susceptibility gene encoding a protein homologous to ULP1 protease in wheat, which may regulate SUMO function facilitating powdery mildew attack. Some plant genes that are required for susceptibilities to certain pathogens are known as susceptibility genes or susceptibility factors, whose loss-of-function mutations can confer the plants resistances. To identify potential susceptibility genes to powdery mildew in wheat, differentially expressed genes in compatible and incompatible interactions between wheat and powdery mildew were examined by the cDNA chip assay. The genes exclusively expressed in the susceptible cultivar were interfered using biolistic transient transformation in wheat epidermal cells. The suppression of gene TaS3 (Triticum aestivum susceptibility) decreased the pathogen penetration by 19%, and its over-expression increased the disease susceptibility. The deduced protein from TaS3 belongs to the putative ubiquitin-like protease 1 peptidase domain family. Subcellular localization studies revealed that its protein was accumulated in the nucleus. Quantitative real-time polymerase chain reaction analysis revealed that TaS3 transcript was significantly induced in the compatible host. This suggests that TaS3 is a potential susceptible gene and its function may be related to regulate SUMO functions.
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Shaohui
authors.initials: S
authors.lastname: Li
authors.forename: Rui
authors.initials: R
authors.lastname: Ji
authors.forename: Robert
authors.initials: R
authors.lastname: Dudler
authors.forename: Mingli
authors.initials: M
authors.lastname: Yong
authors.forename: Qide
authors.initials: Q
authors.lastname: Deng
authors.forename: Zhengyi
authors.initials: Z
authors.lastname: Wang
authors.forename: Dongwei
authors.initials: D
authors.lastname: Hu
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000502:physiology*; D003001:Cloning, Molecular; D004198:Disease Susceptibility; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D017930:Genes, Reporter; D056726:Genetic Association Studies; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D019683:Penetrance; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D014908:Triticum / Q000235:genetics* / Q000382:microbiology*
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins