title: Facile and fast detection of bacteria via the detection of exogenous volatile metabolites released by enzymatic hydrolysis.
abstract: A low-cost, innovative and non-invasive colorimetric test, which can be universally used, is proposed to detect pathogenic bacteria via the simple and fast detection of volatile metabolites released by enzymatic hydrolysis. The proof of concept is shown via three sets of experiments studying the release of the p-nitrophenol metabolite in solution in the E. coli cultures containing 4-nitrophenyl-β-d-glucuronide, the trapping efficiency of the gaseous metabolite by various tailored and functionalized xerogels, and the trapping and detection of gaseous p-nitrophenol released by E. coli bacteria.
authors.affiliation: CEA LETI MINATEC, Department of Technology for Biology and Healthcare, 17 avenue des Martyrs, Grenoble, F-38054, France. pierre.marcoux@cea.fr.
authors.forename: Laure-Hélène
authors.initials: LH
authors.lastname: Guillemot
authors.forename: Marjorie
authors.initials: M
authors.lastname: Vrignaud
authors.forename: Pierre R
authors.initials: PR
authors.lastname: Marcoux
authors.forename: Charles
authors.initials: C
authors.lastname: Rivron
authors.forename: Thu-Hoa
authors.initials: TH
authors.lastname: Tran-Thi
mesh_terms: D002849:Chromatography, Gas; D003124:Colorimetry; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D005740:Gases / Q000737:chemistry; D005782:Gels / Q000737:chemistry; D005965:Glucuronates / Q000737:chemistry / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009596:Nitrophenols / Q000737:chemistry / Q000378:metabolism*; D016062:Porosity; D014835:Volatilization
chemical_list: D005740:Gases; D005782:Gels; D005965:Glucuronates; D009596:Nitrophenols; C002730:4-nitrophenylglucuronide; D005966:Glucuronidase; C024836:4-nitrophenol