title: Molecular cloning and expression pattern of 11 genes involved in lipid metabolism in yellow catfish Pelteobagrus fulvidraco.
abstract: 11 genes involved in lipid metabolism were cloned from liver of yellow catfish Pelteobagrus fulvidraco, including CPT 1A, CPT 1B, PPARα, PPARγ, SREBP-1, G6PD, 6PGD, FAS, acetyl-CoA ACCa, ACCb, and LPL. Phylogenetic analysis further identified these genes, and confirmed the classification and evolutionary status of yellow catfish. mRNA of all eleven genes was present in liver, muscle, mesenteric adipose, ovary and heart, but at varying levels. The present study will facilitate further studies on the regulation of lipid metabolism at the molecular level for the fish species.
authors.affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, Wuhan 430070, China; Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan 430070, China.
authors.forename: Jia-Lang
authors.initials: JL
authors.lastname: Zheng
authors.forename: Zhi
authors.initials: Z
authors.lastname: Luo
authors.forename: Qing-Ling
authors.initials: QL
authors.lastname: Zhu
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Tan
authors.forename: Qi-Liang
authors.initials: QL
authors.lastname: Chen
authors.forename: Lin-Dan
authors.initials: LD
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002397:Catfishes / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D050356:Lipid Metabolism / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny
keywords: 6-phosphogluconate dehydrogenase; 6PGD; ACC; CPT I; EF1α; FAS; G6PD; GAPDH; Gene cloning; HPRT; LPL; Lipid metabolism; MS-222; NJ; ORF; PCR; PPAR; Pelteobagrus fulvidraco; SEM; SREBP; Tissue expression; acetyl-CoA carboxylase; carnitine palmitoyltransferase; elongation factor 1 alpha; fatty acid synthase; glucose 6-phosphate dehydrogenase; glyceraldehydes 3-phosphate dehydrogenase; hypoxanthine-guanine phosphoribosyl transferase; lipoprotein lipase; neighbor-joining; open reading frame; peroxisome proliferators-activated receptor; polymerase chain reaction; standard error of mean; sterol-regulator element-binding protein; tricaine methanesulfonate