title: Direct and selective tagging of cysteine residues in peptides and proteins with 4-nitropyridyl lanthanide complexes.
abstract: A cysteine-selective tagging method in water is reported, based on rapid displacement of a pyridyl nitro-substituent in simple pyridines and lanthanide complexes. The conjugation reaction creates a short link between the tag and peptide, holding the peptide closer to the Ln(3+) ion and with reduced flexibility compared to existing methods.
authors.affiliation: Department of Chemistry, Durham University, South Road, Durham, DH1 3LE, UK. s.j.butler@dur.ac.uk david.parker@dur.ac.uk.
authors.forename: Kathryn L
authors.initials: KL
authors.lastname: Gempf
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Butler
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Funk
authors.forename: David
authors.initials: D
authors.lastname: Parker
mesh_terms: D056831:Coordination Complexes / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D011725:Pyridines / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D007477:Ions; D028581:Lanthanoid Series Elements; D010455:Peptides; D011506:Proteins; D011725:Pyridines; D003545:Cysteine; C023666:pyridine