title: Molecular characterization of T cell receptor beta variable in the peripheral blood T cell repertoire in subjects with active tuberculosis or latent tuberculosis infection.
abstract: BACKGROUND
  T cells are closely linked to the clinical manifestations of subjects with Mycobacterium tuberculosis (MTB) infection. T cell receptor beta variable (TCRBV) is a signal and indicative molecule on the membrane of T lymphocytes, reflecting the composition and specificity of T cells. The molecular profiles of TCRBV in peripheral blood mononuclear cells (PBMCs) and their subpopulations (CD4+ and CD8+ T cells) from subjects with active tuberculosis (TB) or latent TB infection (LTBI) have not been well described.
  
  
  METHODS
  In 42 subjects with active TB or LTBI, PMBCs and their subsets were separated and sorted. The molecular profiles of the TCRBV complementarity determining region 3 (CDR3) in the three cell populations were investigated using our recently developed gene melting spectral pattern (GMSP) assay. The TCRBV members were then cloned and sequenced when their GMSP image profiles showed a single-peak.
  
  
  RESULTS
  The average number of skewed TCRBV molecules in the CD4+ cell subset was significantly higher than that in PBMCs and CD8+ T cells. TCRBV12, BV13.1, BV13.2, and BV24 were expressed more prevalently than other TCRBV gene families in the three cell populations. In addition, relatively conserved amino acid motifs were identified in TCRBV5.1 and BV20 CDR3 in PBMCs and its subsets. The monoclonal TCRBV14 and BV23 expressed were different between active TB and LTBI subjects.
  
  
  CONCLUSIONS
  These results indicate that the T cell immune response is complex and multi-specific in active TB and LTBI subjects. Analysis of TCRBV expression in CD4+ T cells suggest that it could be useful in assessing the composition and status of circulating T cells. Furthermore, the expression of TCRBV14, BV23 and the sequencing of CDR3 amino acid motifs of TCRBV5.1, BV20 could be used in the differential diagnosis and treatment of subjects with active TB or LTBI.
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Disease, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China. ljli@zju.edu.cn.
authors.forename: Jiezuan
authors.initials: J
authors.lastname: Yang
authors.forename: Jianqin
authors.initials: J
authors.lastname: He
authors.forename: Haijun
authors.initials: H
authors.lastname: Huang
authors.forename: Zhongkang
authors.initials: Z
authors.lastname: Ji
authors.forename: Li
authors.initials: L
authors.lastname: Wei
authors.forename: Ping
authors.initials: P
authors.lastname: Ye
authors.forename: Kaijin
authors.initials: K
authors.lastname: Xu
authors.forename: Lanjuan
authors.initials: L
authors.lastname: Li
mesh_terms: D000328:Adult; D020816:Amino Acid Motifs; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D014644:Genetic Variation; D006801:Humans; D055985:Latent Tuberculosis / Q000235:genetics* / Q000276:immunology; D007963:Leukocytes, Mononuclear; D008297:Male; D008875:Middle Aged; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D014376:Tuberculosis / Q000235:genetics* / Q000276:immunology; D055815:Young Adult
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta