title: Species identification and evolutionary inference of the genera Megalobrama and Parabramis (Cyprinidae: Cultrinae) in China.
abstract: The species boundaries and evolutionary relationships of two closely related genera, Megalobrama and Parabramis, were inferred from the partial mitochondrial cytochrome oxidase subunit I (COI) gene, NADH dehydrogenase subunit 2 (ND2) gene and their concatenated segment. Phylogenetic reconstructions showed that among the three breams, Megalobrama amblycephala and Megalobrama skolkovii are more closely related to each other than either is to Megalobrama terminalis. The taxonomy of M. pellegrini should be reconsidered. The divergence time estimation based on the assumption of a global molecular clock indicated that speciation and dispersal of the two genera might have occurred at approximately Pliocene to Late Pleistocene, due to major paleo-environmental events associated with monsoon evolution and the formation of the Three Gorges of the Yangtze River.
authors.affiliation: College of Fishery, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University , Wuhan , P. R. China .
authors.forename: X H
authors.initials: XH
authors.lastname: Bai
authors.forename: X W
authors.initials: XW
authors.lastname: Guo
authors.forename: X J
authors.initials: XJ
authors.lastname: Zhang
authors.forename: W
authors.initials: W
authors.lastname: Song
authors.forename: Y H
authors.initials: YH
authors.lastname: Li
authors.forename: W
authors.initials: W
authors.lastname: Luo
authors.forename: X J
authors.initials: XJ
authors.lastname: Cao
authors.forename: W M
authors.initials: WM
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D003530:Cyprinidae / Q000145:classification* / Q000235:genetics; D004247:DNA / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000235:genetics; D019143:Evolution, Molecular; D008040:Genetic Linkage; D006239:Haplotypes; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D004247:DNA; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV
keywords: COI; ND2; molecular clock; mtDNA; phylogenetic relationships; species validity