title: Accelerating the design of biomimetic materials by integrating RNA-seq with proteomics and materials science.
abstract: Efforts to engineer new materials inspired by biological structures are hampered by the lack of genomic data from many model organisms studied in biomimetic research. Here we show that biomimetic engineering can be accelerated by integrating high-throughput RNA-seq with proteomics and advanced materials characterization. This approach can be applied to a broad range of systems, as we illustrate by investigating diverse high-performance biological materials involved in embryo protection, adhesion and predation. In one example, we rapidly engineer recombinant squid sucker ring teeth proteins into a range of structural and functional materials, including nanopatterned surfaces and photo-cross-linked films that exceed the mechanical properties of most natural and synthetic polymers. Integrating RNA-seq with proteomics and materials science facilitates the molecular characterization of natural materials and the effective translation of their molecular designs into a wide range of bio-inspired materials.
authors.affiliation: 1] School of Materials Science and Engineering, Nanyang Technological University, Singapore. [2].
authors.forename: Paul A
authors.initials: PA
authors.lastname: Guerette
authors.forename: Shawn
authors.initials: S
authors.lastname: Hoon
authors.forename: Yiqi
authors.initials: Y
authors.lastname: Seow
authors.forename: Manfred
authors.initials: M
authors.lastname: Raida
authors.forename: Admir
authors.initials: A
authors.lastname: Masic
authors.forename: Fong T
authors.initials: FT
authors.lastname: Wong
authors.forename: Vincent H B
authors.initials: VH
authors.lastname: Ho
authors.forename: Kiat Whye
authors.initials: KW
authors.lastname: Kong
authors.forename: Melik C
authors.identifier: 0000000304667649
authors.initials: MC
authors.lastname: Demirel
authors.forename: Abdon
authors.initials: A
authors.lastname: Pena-Francesch
authors.forename: Shahrouz
authors.initials: S
authors.lastname: Amini
authors.forename: Gavin Z
authors.initials: GZ
authors.lastname: Tay
authors.forename: Dawei
authors.initials: D
authors.lastname: Ding
authors.forename: Ali
authors.initials: A
authors.lastname: Miserez
mesh_terms: D000268:Adhesiveness; D000595:Amino Acid Sequence; D000825:Animal Structures / Q000648:ultrastructure; D000818:Animals; D059001:Aquatic Organisms / Q000378:metabolism; D040761:Biomimetic Materials / Q000737:chemistry*; D032701:Biomimetics / Q000379:methods*; D008969:Molecular Sequence Data; D010063:Ovum / Q000378:metabolism; D015202:Protein Engineering; D040901:Proteomics / Q000379:methods*; D011994:Recombinant Proteins / Q000737:chemistry; D017423:Sequence Analysis, RNA; D047011:Silk / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D011994:Recombinant Proteins; D047011:Silk