title: The PCP protein Vangl2 regulates migration of hindbrain motor neurons by acting in floor plate cells, and independently of cilia function.
abstract: Vangl2, a core component of the Planar Cell Polarity pathway, is necessary for the caudal migration of Facial Branchiomotor (FBM) neurons in the vertebrate hindbrain. Studies in zebrafish suggest that vangl2 functions largely non-cell autonomously to regulate FBM neuron migration out of rhombomere 4 (r4), but the cell-type within which it acts is not known. Here, we demonstrate that vangl2 functions largely in floor plate cells to regulate caudal neuronal migration. Furthermore, FBM neurons fail to migrate caudally in the mouse Gli2 mutant that lacks the floor plate, suggesting an evolutionarily conserved role for this cell type in neuronal migration. Although hindbrain floor plate cilia are disorganized in vangl2 mutant embryos, cilia appear to be dispensable for neuronal migration. Notably, Vangl2 is enriched in the basolateral, but not apical, membranes of floor plate cells. Taken together, our data suggest strongly that Vangl2 regulates FBM neuron migration by acting in floor plate cells, independently of cilia function.
authors.affiliation: Division of Biological Sciences, and Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Vinoth
authors.initials: V
authors.lastname: Sittaramane
authors.forename: Xiufang
authors.initials: X
authors.lastname: Pan
authors.forename: Derrick M
authors.initials: DM
authors.lastname: Glasco
authors.forename: Peng
authors.initials: P
authors.lastname: Huang
authors.forename: Suman
authors.initials: S
authors.lastname: Gurung
authors.forename: Anagha
authors.initials: A
authors.lastname: Bock
authors.forename: Shike
authors.initials: S
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Koichi
authors.initials: K
authors.lastname: Kawakami
authors.forename: Michael P
authors.initials: MP
authors.lastname: Matise
authors.forename: Anand
authors.initials: A
authors.lastname: Chandrasekhar
mesh_terms: D000818:Animals; D002465:Cell Movement; D016764:Cell Polarity; D002923:Cilia / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D009046:Motor Neurons / Q000378:metabolism*; D055495:Neurogenesis; D012249:Rhombencephalon / Q000166:cytology / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism; D000075746:Zinc Finger Protein Gli2
chemical_list: C000618551:Gli2 protein, mouse; D051741:Kruppel-Like Transcription Factors; D008565:Membrane Proteins; D029961:Zebrafish Proteins; D000075746:Zinc Finger Protein Gli2; C491759:vangl2 protein, zebrafish
keywords: Cilia; Facial Branchiomotor Neuron; Floor plate cells; Hindbrain; Neuronal migration; Van gogh-like 2