title: Enrichment, development, and assessment of Indian basil oil based antiseptic cream formulation utilizing hydrophilic-lipophilic balance approach.
abstract: The present work was aimed to develop an antiseptic cream formulation of Indian basil oil utilizing hydrophilic-lipophilic balance approach. In order to determine the required-hydrophilic lipophilic balance (rHLB) of basil oil, emulsions of basil oil were prepared by phase inversion temperature technique using water, Tween 80, and Span 80. Formulated emulsions were assessed for creaming (BE9; 9.8, BE10; 10.2), droplet size (BE18; 3.22 ± 0.09 μ m), and turbidity (BE18; 86.12 ± 2.1%). To ensure correctness of the applied methodology, rHLB of light liquid paraffin was also determined. After rHLB determination, basil oil creams were prepared with two different combinations of surfactants, namely, GMS : Tween 80 (1 : 3.45) and SLS : GMS (1 : 3.68), and evaluated for in vitro antimicrobial activity, skin irritation test, viscosity and consistency. The rHLB of basil oil and light liquid paraffin were found to be 13.36 ± 0.36 and 11.5 ± 0.35, respectively. Viscosity, and consistency parameters of cream was found to be consistent over 90 days. Cream formulations showed net zone of growth inhibition in the range of 5.0-11.3 mm against bacteria and 4.3-7.6 mm against fungi. Primary irritation index was found to be between 0.38 and1.05. Conclusively stable, consistent, non-irritant, enriched antiseptic basil oil cream formulations were developed utilizing HLB approach.
authors.affiliation: Herbal Medicinal Products Department, CSIR-Central Institute of Medicinal and Aromatic Plants, PO-CIMAP, Lucknow 226015, India. npyadav@gmail.com
authors.forename: Narayan Prasad
authors.initials: NP
authors.lastname: Yadav
authors.forename: Jaya Gopal
authors.initials: JG
authors.lastname: Meher
authors.forename: Neelam
authors.initials: N
authors.lastname: Pandey
authors.forename: Suaib
authors.initials: S
authors.lastname: Luqman
authors.forename: Kuldeep Singh
authors.initials: KS
authors.lastname: Yadav
authors.forename: Debabrata
authors.initials: D
authors.lastname: Chanda
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002626:Chemistry, Pharmaceutical; D004655:Emulsions; D057927:Hydrophobic and Hydrophilic Interactions / Q000187:drug effects*; D007194:India; D008055:Lipids / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D009391:Nephelometry and Turbidimetry; D031339:Ocimum; D010232:Paraffin; D010316:Particle Size; D010938:Plant Oils / Q000494:pharmacology*; D011817:Rabbits; D012867:Skin / Q000187:drug effects; D023422:Skin Irritancy Tests; D014783:Viscosity
chemical_list: D000890:Anti-Infective Agents; D000891:Anti-Infective Agents, Local; D004655:Emulsions; D008055:Lipids; D010938:Plant Oils; D010232:Paraffin; C051168:basil oil