title: [Effect of genistein on rat femoral bone metabolic activity in vitro].
abstract: This study is to investigate effects of genistein on rat femoral bone metabolic in vitro. Rat femoral tissues was isolated and randomly divided into two groups including control group and genistein (1 x 10(-5) mol x(-1)) group. Determinations of alkaline phosphatase (ALP) activity, calcium content and osteoprotegerin (OPG), type I-collagen (Collagen-I), RANKL, Runx-2 and bone morphogenetic protein (BMP-2) mRNA expression were done by real-time PCR. The results showed that 1 x 10(-5) mol x L(-1) genistein could increase the activity of ALP and contents of Ca, regulate bone metabolism activity of OPG, RANKL, BMP-2, Collagen-I and Runx-2 mRNA expression level. Genistein can significantly modulate bone metabolism related gene expression level of rat femoral tissue in vitro, and can increase calcium content and the activity of ALP.
authors.affiliation: Institute of Orthopaedics, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.forename: Bao-Feng
authors.initials: BF
authors.lastname: Ge
authors.forename: Ke-Ming
authors.initials: KM
authors.lastname: Chen
authors.forename: Xiao-Ni
authors.initials: XN
authors.lastname: Ma
authors.forename: Kui
authors.initials: K
authors.lastname: Cheng
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Guo
authors.forename: Xiang
authors.initials: X
authors.lastname: Lü
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005269:Femur / Q000378:metabolism*; D005786:Gene Expression Regulation; D019833:Genistein / Q000494:pharmacology*; D053244:Osteoprotegerin / Q000235:genetics / Q000378:metabolism; D048789:Phytoestrogens / Q000494:pharmacology*; D053245:RANK Ligand / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D053244:Osteoprotegerin; D048789:Phytoestrogens; D053245:RANK Ligand; D012333:RNA, Messenger; C493717:Runx2 protein, rat; C506032:Tnfrsf11b protein, rat; D019833:Genistein; D000469:Alkaline Phosphatase; D002118:Calcium