title: Effect of activated autologous monocytes/macrophages on wound healing in a rodent model of experimental diabetes.
abstract: OBJECTIVE
  We aimed to evaluate the effectiveness of the application of activated autologous monocytes/macrophages (Mo/Mp) on wound healing in diabetic rats.
  
  
  METHODS
  Sixty male SD rats were equally divided into the following: control group (normal, nondiabetic), PBS-treated diabetic group, and tumor necrotic factor alpha (TNF-α) plus interferon-γ (IFN-γ)-stimulated or unstimulated Mo/Mp-treated diabetic group. Full-thickness round wounds (1cm×1cm) were created in the right hind foot of rats and the wounds were treated with PBS or Mo/Mp on day 1 after injury. In the following 14 days, the percentage of wound contraction was measured, histologic examination was performed with hematoxylin and eosin staining, and vascular endothelial growth factor (VEGF) in the wound was evaluated by Western blot analysis.
  
  
  RESULTS
  Diabetic rats exhibited impaired wound healing with delayed angiogenesis and VEGF expression. The early application of TNF-α plus IFN-γ-stimulated autologous Mo/Mp to diabetic wounds significantly improved the delayed wound healing through the stimulation of angiogenesis and re-epithelization, as well as restoring the defect in VEGF expression.
  
  
  CONCLUSIONS
  Mo/Mp activated by TNF-α and IFN-γ promotes diabetic wound healing and normalizes the defect in VEGF regulation associated with diabetes-induced skin-repair disorders.
authors.affiliation: Department of Rehabilitation, The 454th Hospital of Chinese PLA, Nanjing 210002, China.
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Gu
authors.forename: Su-e
authors.initials: SE
authors.lastname: Shen
authors.forename: Chong-fa
authors.initials: CF
authors.lastname: Huang
authors.forename: Ying-na
authors.initials: YN
authors.lastname: Liu
authors.forename: Yin-chen
authors.initials: YC
authors.lastname: Chen
authors.forename: Ling
authors.initials: L
authors.lastname: Luo
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zeng
authors.forename: Ai-ping
authors.initials: AP
authors.lastname: Wang
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D007371:Interferon-gamma / Q000494:pharmacology; D008264:Macrophages / Q000502:physiology* / Q000637:transplantation; D008297:Male; D009000:Monocytes / Q000502:physiology* / Q000637:transplantation; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; D007371:Interferon-gamma
keywords: Diabetes; Foot; Monocytes/macrophages; Rat; Wound