title: Isocitric acid production from rapeseed oil by Yarrowia lipolytica yeast.
abstract: Production of D S-threo-isocitric acid (ICA) by yeast meets serious difficulties since it is accompanied by a simultaneous production of citric acid (CA) in significant amounts that reduces the yield of desired product. In order to develop an effective process of ICA production, 60 yeast strains of different genera (Candida, Pichia, Saccharomyces, Torulopsis, and Yarrowia) were tested for their ability to produce ICA from rapeseed oil; as a result, wild-type strain Yarrowia lipolytica VKM Y-2373 and its mutant Y. lipolytica 704-UV4-A/NG50 were selected as promising ICA producers. The effects of temperature, pH, aeration, and concentrations of rapeseed oil, iron, and itaconic acid on ICA production by selected strains were studied. Under optimal conditions (pH 6.0; aeration 50-55 %; rapeseed oil concentration of 20-60 gl(-1), iron ion concentration of 1.2 mg l(-1), and itaconic acid amount of 30 mM), selected strains of Y. lipolytica produced predominantly ICA with a low amount of a by-product, CA.
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki 5, Pushchino, Moscow Region, 142290, Russia, kamzolova@rambler.ru.
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Kamzolova
authors.forename: Emiliya G
authors.initials: EG
authors.lastname: Dedyukhina
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Samoilenko
authors.forename: Julia N
authors.initials: JN
authors.lastname: Lunina
authors.forename: Irina F
authors.initials: IF
authors.lastname: Puntus
authors.forename: Ramil L
authors.initials: RL
authors.lastname: Allayarov
authors.forename: Maria N
authors.initials: MN
authors.lastname: Chiglintseva
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Mironov
authors.forename: Igor G
authors.initials: IG
authors.lastname: Morgunov
mesh_terms: D003470:Culture Media / Q000378:metabolism; D005229:Fatty Acids, Monounsaturated; D007523:Isocitrates / Q000378:metabolism*; D010938:Plant Oils / Q000378:metabolism*; D000074262:Rapeseed Oil; D025062:Yarrowia / Q000378:metabolism*; D015003:Yeasts / Q000378:metabolism
chemical_list: D003470:Culture Media; D005229:Fatty Acids, Monounsaturated; D007523:Isocitrates; D010938:Plant Oils; D000074262:Rapeseed Oil; C034219:isocitric acid