title: Mycobacterium bourgelatii sp. nov., a rapidly growing, non-chromogenic species isolated from the lymph nodes of cattle.
abstract: Three independent strains of a rapidly growing, non-chromogenic member of the genus Mycobacterium were isolated from lymph nodes of French cattle. Identification of the isolates was carried out using a polyphasic approach. The nearly complete SSU rRNA gene sequences (>1200 bp) of the strains MLB-A23, MLB-A30 and MLB-A84(T) were identical. A phylogenetic analysis of these unique SSU rRNA gene sequences showed that these strains were most closely related to Mycobacterium intermedium. Further phylogenetic analysis based on concatenated sequences (2854 bp) of four housekeeping genes (hsp65, rpoB, sodA and tuf), the transfer-messenger RNA (tmRNA) and SSU rRNA genes indicated that these three strains represented a distinct species that shares a common ancestor with M. intermedium. Phylogenetic and phenotypic data strongly indicate that the strains MLB-A23, MLB-A30 and MLB-A84(T) belong to a novel mycobacterial species for which the name Mycobacterium bourgelatii sp. nov. is proposed. The type strain is MLB-A84(T) ( = CIP 110557(T) = DSM 45746(T)).
authors.affiliation: VetAgro Sup Campus Vétérinaire de Lyon, Département de Santé Publique Vétérinaire, Marcy l'Étoile, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Guérin-Faublée
authors.affiliation: Université de Lyon, Université Lyon 1, CNRS UMR5558, Laboratoire de Biométrie et Biologie Evolutive, Villeurbanne, France.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Flandrois
authors.affiliation: Hospices Civils de Lyon, Centre Hospitalier Lyon Sud, Laboratoire de Bactériologie, Pierre Bénite, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Pichat
authors.affiliation: Agence Nationale de Sécurité Sanitaire de l'Alimentation, du Travail et de l'Environnement, Laboratoire de Santé Animale de Maisons-Alfort, Unité Zoonoses Bactériennes, Maisons-Alfort, France.
authors.forename: Maria Laura
authors.initials: ML
authors.lastname: Boschiroli
authors.affiliation: Université Montpellier 1, CNRS UMR 5119, Equipe Pathogènes et Environnements, Montpellier, France.|Centre Hospitalier de Montpellier, Hôpital Arnaud de Villeneuve, Laboratoire de Bactériologie, Montpellier, France.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Lamy
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002417:Cattle / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005602:France; D005798:Genes, Bacterial; D008198:Lymph Nodes / Q000382:microbiology*; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009171:Mycolic Acids / Q000737:chemistry; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D054680:Ribosome Subunits, Small, Bacterial / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D009171:Mycolic Acids; D012329:RNA, Bacterial; C096222:tmRNA