title: Chronic intermittent hypoxia preserves bone density in a mouse model of sleep apnea.
abstract: Very recent clinical research has investigated whether obstructive sleep apnea (OSA) may modulate bone homeostasis but the few data available are conflicting. Here we report novel data obtained in a mouse study specifically designed to determine whether chronic intermittent hypoxia realistically mimicking OSA modifies bone mineral density (BMD). Normal male and female mice and orchidectomized mice (N=10 each group) were subjected to a pattern of high-frequency intermittent hypoxia (20s at 5% and 40s at 21%, 60 cycles/h) for 6h/day. Identical groups breathing room air (normoxia) were the controls. After 32 days of intermittent hypoxia/normoxia the trabecular bone mineral density (BMD) in the peripheral femora were measured by micro-CT scanning. When compared with normoxia (two-way ANOVA), intermittent hypoxia did not significantly modify BMD in the three animal groups tested. Data in this study suggest that the type of intermittent hypoxia characterizing OSA, applied as a single challenge, preserves bone homeostasis.
authors.affiliation: CIBER Enfermedades Respiratorias, Bunyola, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Torres
authors.forename: Josep M
authors.initials: JM
authors.lastname: Montserrat
authors.forename: Javier
authors.initials: J
authors.lastname: Pavía
authors.forename: Mireia
authors.initials: M
authors.lastname: Dalmases
authors.forename: Domenec
authors.initials: D
authors.lastname: Ros
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Fernandez
authors.forename: Ferran
authors.initials: F
authors.lastname: Barbé
authors.forename: Daniel
authors.initials: D
authors.lastname: Navajas
authors.forename: Ramon
authors.initials: R
authors.lastname: Farré
mesh_terms: D015502:Absorptiometry, Photon; D000704:Analysis of Variance; D000818:Animals; D015519:Bone Density; D001847:Bone Diseases / Q000209:etiology* / Q000517:prevention & control*; D004195:Disease Models, Animal; D005260:Female; D000860:Hypoxia / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012727:Sex Characteristics; D012891:Sleep Apnea Syndromes / Q000150:complications*; D018709:Statistics, Nonparametric
keywords: Animal model; Bone metabolism; Bone mineral density; Intermittent hypoxia; Obstructive sleep apnea; Osteoporosis