title: [Effects of platelet-rich fibrin extract on MC3T3-E1 cell].
abstract: OBJECTIVE
  To evaluate the effect of platelet-rich fibrin extract (PRFe) on proliferation and differentiation and F-actin cytoskeleton of osteoblasts.
  
  
  METHODS
  The experimental group used the α-minimum essential medium (α-MEM) containing PRFe (10% fetal bovine serum), and the control group used the α-MEM (10% fetal bovine serum). The number of the osteoblasts at 1st, 3rd, 5th d was detected by methyl thiazolyl tetrazolium (MTT) assay, and the differentiation of osteoblast at 1st, 3rd, 5th,7 th d detected by the activity of alkaline phosphatase (ALP).The alizarin red dye was used to observe the number of calcium nodus at 14th, 21st d. The F-actin cytoskeleton was evaluated by confocal laser scanning microscope(CLSM) at 3rd,6th,9th,12th h. The level of osteogenetic biomarkers osteocalcin (OCN) and core-binding factor α1(Cbfα1) at 3rd,7th d were quantified by real-time PCR.
  
  
  RESULTS
  A significant increase of absorbance at 1st, 3rd, 5th d was showed in experimental group (0.336 ± 0.011, 0.571 ± 0.039, 0.787 ± 0.050) compared to control group (0.300 ± 0.021, 0.387 ± 0.040, 0.527 ± 0.034) (P < 0.05). The absorbance of experimental group at 1st, 3rd, 5th, 7th d (0.146 ± 0.014, 0.199 ± 0.017, 0.390 ± 0.020, 0.492 ± 0.019) was significantly higher than that of control group (0.115 ± 0.014, 0.145 ± 0.015, 0.190 ± 0.015, 0.230 ± 0.026) (P < 0.05). The integrated absorbance of the calcium nodus in experimental group at 14th, 21st d (22.119 ± 3.694, 31.528 ± 3.162) was significantly higher than in control group (8.498 ± 2.041, 15.162 ± 2.526) (P < 0.05). The Cbfα1 and OCN gene expression in experimental group was higher than in control group (P < 0.05).
  
  
  CONCLUSIONS
  PRFe could enhance the proliferation and differentiation of osteoblasts and promote the spread of F-actin cytoskeleton.
authors.affiliation: Department of Dental Implantology, Yantai Stomatological Hospital, Yantai Shandong, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Dong
authors.forename: Zhong-hao
authors.initials: ZH
authors.lastname: Liu
authors.forename: Xiao-jie
authors.initials: XJ
authors.lastname: Zhang
authors.forename: Feng-wei
authors.initials: FW
authors.lastname: Xu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D003599:Cytoskeleton / Q000187:drug effects*; D005337:Fibrin / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D015675:Osteocalcin / Q000378:metabolism; D053657:Platelet-Rich Plasma / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D015675:Osteocalcin; D005337:Fibrin; D000469:Alkaline Phosphatase