title: Quantification of taxanes in a leaf and twig extract from Taxus baccata L. using 13C NMR spectroscopy.
abstract: In the course of our ongoing work on the chemical characterization of Taxus baccata L. growing wild in Corsica, we have developed and validated a method for direct quantification of taxane derivatives by (13)C NMR using 10-deacetylbaccatin III as reference compound and 1,6-hexanediol as internal standard. We have observed good accuracy (relative errors between 0.3% and 3.5%), linearity (R(2) = 0.999) and precision (reproducibility 8.5 mg ± 1.1%) of the measurements. The experimental procedure was applied to the quantification of six identified taxanes in a fraction of chromatography of a methanol extract of T. baccata leaves. This method can be applied to other compounds bearing the taxane skeleton.
authors.affiliation: Université de Corse, UMR CNRS 6134 SPE, Equipe Chimie et Biomasse, Route des Iles Sanguinaires, 20000, Ajaccio, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Paoli
authors.forename: Ange
authors.initials: A
authors.lastname: Bighelli
authors.forename: Vincent
authors.initials: V
authors.lastname: Castola
authors.forename: Félix
authors.initials: F
authors.lastname: Tomi
authors.forename: Joseph
authors.initials: J
authors.lastname: Casanova
keywords: 13C NMR; Taxus baccata L; quantification; taxanes