title: Novel xylanases from Simplicillium obclavatum MTCC 9604: comparative analysis of production, purification and characterization of enzyme from submerged and solid state fermentation.
abstract: The production of extracellular xylanase by a newly isolated fungus Simplicillium obclavatum MTCC 9604 was studied in solid-state and submerged fermentation. Multiple xylanases and endoglucanases were produced by the strain during growth on wheat bran in solid state fermentation (SSF). A single xylanase isoform was found to be produced by the same fungus under submerged fermentation (SF) using wheat bran as sole carbon source. Enzyme activity, stability and the protein yield were much higher in SSF than SF. The two dimensional zymogram of the crude enzyme indicated the presence of six isoforms with different pI values starting from pH 3-10. The optimum temperature and pH for the partially purified xylanase activity were 50°C and pH 5.0 respectively; xylanase enzymes exhibited remarkable stability over a broad pH range and the temperature range of 30-60°C which has great potential to be used in biofuels, animal feed and food industry applications.
authors.affiliation: Department of Biochemistry, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700 019 West Bengal India.
authors.forename: Saugata
authors.initials: S
authors.lastname: Roy
authors.forename: Tanmay
authors.initials: T
authors.lastname: Dutta
authors.forename: Tuhin Subhra
authors.initials: TS
authors.lastname: Sarkar
authors.forename: Sanjay
authors.initials: S
authors.lastname: Ghosh
keywords: Simplicillium obclavatum; Solid state fermentation; Submerged fermentation; Xylanase