title: Qualitative and quantitative analysis of the constituents in Danmu preparations by UPLC-PDA-TOF-MS.
abstract: An ultra performance liquid chromatography coupled with photodiode array detector and time-of-flight tandem mass spectrometry (UPLC-PDA-TOF-MS) method was developed for the quality assessment of Danmu preparations, a commonly used traditional Chinese medicine. Thirty-three compounds from Danmu preparations were simultaneously detected; among them, 14 compounds were unequivocally identified based on their retention behaviors, UV spectrum, MS and MS(n) data by comparing with reference substances, and the others were tentatively characterized by literatures. Twelve of 33 compounds were simultaneously determined by UPLC-PDA, and the validation of the quantitative method, including recoveries, linearity, sensitivity, precision and repeatability was carried out and the results demonstrated to be satisfied the requirements of quantitative analysis. The results suggested that the established method would be a powerful and reliable analytical tool for quality control of Danmu preparations and the characterization of multi-constituent in complex chemical system.
authors.affiliation: Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, PR China zfxcjq@126.com.
authors.forename: Fenxia
authors.initials: F
authors.lastname: Zhu
authors.affiliation: School of Sciences, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Jiaquan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, PR China School of Sciences, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Yin
authors.affiliation: Hainan Pharmaceutical Factory Co, LTD, Wuzhishan 572200, PR China.
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, PR China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Jia
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; C000602095:danmu