title: Determination of chloramphenicol residues in aquatic products using immunoaffinity column cleanup and high performance liquid chromatography with ultraviolet detection.
abstract: A method based on HPLC with UV detection was developed for the quantitative determination of chloramphenicol (CAP) residues in aquatic products. The samples were extracted with ethyl acetate-ammonium hydroxide (98 + 2, v/v), followed by a cleanup step using an immunoaffinity column. The analytes were determined by HPLC-UV. Optimal conditions for the extraction and cleanup procedures are described. The linear regression equation was y = 91.47x - 8.60 with R = 0.9998 (y = peak area and x = CAP concentration) and showed a good reproducibility. The LOQ was 0.25 microg/kg for determining CAP spiked in the aquatic products. The mean recoveries of CAP from fish and shrimp samples fortified at 0.25-1.0 microg/kg were 88.7-93.1 and 92.0-97.3%, respectively; the repeatability RSDs were less than 8.1%. It was concluded that the method is simple, highly sensitive, and low cost for quantitatively measuring CAP residues in aquatic products. Analyte identification was confirmed by HPLC/MSIMS analysis.
authors.affiliation: University of South China, Department of Chemistry and Chemical Engineering, Hunan, Hengyang 421001, People's Republic of China.
authors.forename: Qing-Jie
authors.initials: QJ
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.forename: Dong-Dong
authors.initials: DD
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Xie
authors.forename: Xiong
authors.initials: X
authors.lastname: Wang
authors.forename: Guo-Min
authors.initials: GM
authors.lastname: Wang
authors.forename: Chang-Ming
authors.initials: CM
authors.lastname: Nie
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D002701:Chloramphenicol / Q000032:analysis*; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D017747:Seafood / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000900:Anti-Bacterial Agents; D002701:Chloramphenicol