title: Development and evaluation of a loop-mediated isothermal amplification assay for diagnosis of Cyprinid herpesvirus 2.
abstract: Goldfish Haematopoietic Necrosis caused by Cyprinid herpesvirus 2 (CyHV-2) is a severe fish disease with high level of mortality. This is the first study on detection of this disease by loop-mediated isothermal amplification (LAMP). A set of six primers targeting terminase gene (accession no. EU349285.1) was determined after a serial of tests. Detection limit was 1.09 × 10(-4)μg/μL, which was superior to conventional PCR and real-time PCR. No cross reaction with 28 other viruses or bacteria commonly found in fish was observed. The application of commercial kit and instrument for the LAMP assay could reduce the risk of cross contamination, which is suitable for detection of infection under field conditions.
authors.affiliation: Shenzhen Exit and Entry Inspection and Quarantine Bureau, Shenzhen, Guangdong 518001, China.
authors.forename: Junqiang
authors.initials: J
authors.lastname: He
authors.forename: Xiujie
authors.initials: X
authors.lastname: Shi
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Xiaocong
authors.initials: X
authors.lastname: Zheng
authors.forename: Wensheng
authors.initials: W
authors.lastname: Lan
authors.forename: Peng
authors.initials: P
authors.lastname: Jia
authors.forename: Jinjin
authors.initials: J
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D005393:Fish Diseases / Q000175:diagnosis* / Q000821:virology*; D006054:Goldfish; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D004279:DNA, Viral
keywords: CyHV-2; Diagnosis; GFHNV; LAMP