title: Low temperature and template-free synthesis of hollow hydroxy zinc phosphate nanospheres and their application in drug delivery.
abstract: Hollow hydroxy zinc phosphate nanospheres (HZnPNSs) with sizes of 30-50 nm and wall thicknesses of about 7 nm were synthesized using a template-free method through wet precipitation of Zn(NO3)2·6H2O and (NH4)2HPO4 at temperatures of 0, 10, and 20 °C. The crystal structures, morphologies, sizes and pore properties, Zn/P molar ratios, and thermal stability properties of nanoparticles have been carefully examined. The methyl-thiotetrazole assay measurements proved the low cell cytotoxicity of the material. The protein adsorption of negatively charged bovine serum albumin (BSA) and positively charged lysozyme on HZnPNSs was also investigated. The results showed that HZnPNSs had high protein adsorption affinity. Furthermore, anticancer doxorubicin as a model drug was used to evaluate the entrapment efficiency and drug loading capacity of HZnPNSs, which showed high loading capacity (>16 wt %) for doxorubicin. The confocal laser scanning microscope observations showed that the drug could be efficiently delivered into cells.
authors.affiliation: Instrumental Analysis Center, Shanghai Jiao Tong University , Shanghai 200240, People's Republic of China.
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Yuan
authors.forename: Bangshang
authors.initials: B
authors.lastname: Zhu
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Ma
authors.forename: Gangsheng
authors.initials: G
authors.lastname: Tong
authors.forename: Yue
authors.initials: Y
authors.lastname: Su
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D016503:Drug Delivery Systems; D051379:Mice; D041681:NIH 3T3 Cells; D054118:Nanospheres / Q000737:chemistry*; D010316:Particle Size; D010710:Phosphates / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D016062:Porosity; D013329:Structure-Activity Relationship; D013499:Surface Properties; D013696:Temperature
chemical_list: D010710:Phosphates