title: Lack of association between let-7 binding site polymorphism rs712 and risk of nasopharyngeal carcinoma.
abstract: Nasopharyngeal carcinoma (NPC) is characterized by its highly invasive and metastatic features. Therefore, screening genetic biomarkers of NPC to achieve early diagnose would be of great value for NPC therapy. Single nucleotide polymorphisms in let-7 miRNA binding site in 3' untranslated region of KRAS mRNA have been found to be associated with various cancer risks. In this study, we genotyped the frequency of KRAS rs712 to test its effect on NPC risk in a hospital-based case-control study in a Chinese population, with 188 histologically confirmed NPC patients and 356 cancer-free controls, using polymerase chain reaction-restriction fragment length polymorphism assay. There was no significant difference in the genotype and allele frequencies of the rs712 polymorphism between the NPC patients and the control group (GT vs. GG, OR 0.83, 95% CI 0.57-1.21; TT vs. GG, OR 1.27, 95% CI 0.58-2.75). Our data suggest that the KRAS rs712 polymorphism in let-7 miRNA binding site has no association with NPC risk. Further experiments with larger sample size or other polymorphism sites are needed to verify the result, especially in different ethnic groups.
authors.affiliation: Department of Forensic Pathology, College of Forensic Medicine, Henan University of Science and Technology, Luoyang, 471003, Henan, People's Republic of China, mitochondrium@126.com.
authors.forename: Xin-Min
authors.initials: XM
authors.lastname: Pan
authors.forename: Jing
authors.initials: J
authors.lastname: Jia
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Guo
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Li
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Hao-Jie
authors.initials: HJ
authors.lastname: Qin
authors.forename: Guo-Hui
authors.initials: GH
authors.lastname: Xu
authors.forename: Lin-Bo
authors.initials: LB
authors.lastname: Gao
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D044466:Asian People / Q000235:genetics; D001665:Binding Sites; D002277:Carcinoma; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C117307:KRAS protein, human; D035683:MicroRNAs; D011518:Proto-Oncogene Proteins; C487931:mirnlet7 microRNA, human; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins