title: Differentiation of genuine Inula britannica L. and substitute specimens based on the determination of 15 components using LC-MS/MS and principal components analysis.
abstract: The aim of this study was to investigate the chemical differences between genuine Inula britannica L. (I. britannica) and substitute specimens. A linear ion trap LC-MS/MS analytical method has been developed for the identification and quantification of 15 major components from I. britannica. Data acquisition was performed in multiple-reaction-monitoring transitions mode followed by an information-dependent acquisition using the enhanced product ion (EPI) scan in one run. The target compounds were further identified and confirmed using an EPI spectral library. The determination results of 45 batches of samples were then analysed and classified by principal component analysis (PCA). The content of 11 components could be used to distinguish the two official Flos Inulae species (I. britannica and Inula japonica) from unofficial species (Inula hupehensis), and the content of 3 components could be used to differentiate the two official species.
authors.affiliation: College of Pharmacy, Hebei Medical University, Shijiazhuang 050017, PR China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Shi
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.forename: Na
authors.initials: N
authors.lastname: Xue
authors.forename: Linfei
authors.initials: L
authors.lastname: Su
authors.forename: Gaixia
authors.initials: G
authors.lastname: Ma
authors.forename: Jinlong
authors.initials: J
authors.lastname: Qi
authors.forename: Yibing
authors.initials: Y
authors.lastname: Wu
authors.forename: Qiao
authors.initials: Q
authors.lastname: Wang
authors.forename: Qingwen
authors.initials: Q
authors.lastname: Shi
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016002:Discriminant Analysis; D038143:Inula / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*; D025341:Principal Component Analysis / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010936:Plant Extracts
keywords: Inula britannica; LC–MS/MS; Principal component analysis; Quality control