title: Streptomyces abietis sp. nov., a cellulolytic bacterium isolated from soil of a pine forest.
abstract: Cellulolytic bacteria A191(T), A192 and A193 isolated from the soil of Sakhalin fir forest in Hokkaido, Japan were studied phenotypically, genotypically and phylogenetically. Analysis of their 16S rRNA gene and gyrB sequences and DNA base composition suggested that these isolates were conspecific and members of the genus Streptomyces. However, levels of 16S rRNA gene and gyrB sequence similarity between the isolates and the type strains of their closest relatives in the genus Streptomyces were no higher than 97.9 and 95.0 %, respectively, implying that these isolates were distinctive. Moreover, the results of DNA-DNA hybridization experiments and physiological characterization clearly differentiated these isolates from their closest neighbours. It is therefore concluded that these isolates represent a novel species of the genus Streptomyces, for which the name Streptomyces abietis is proposed. The type strain is A191(T) ( = NBRC 109094(T) = DSM 42080(T)).
authors.affiliation: Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Fujii
authors.affiliation: National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama 236-8648, Japan.
authors.forename: Masataka
authors.initials: M
authors.lastname: Satomi
authors.affiliation: National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama 236-8648, Japan.
authors.forename: Youhei
authors.initials: Y
authors.lastname: Fukui
authors.affiliation: Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Matsunobu
authors.affiliation: The Center for Instrumental Analysis, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Youji
authors.initials: Y
authors.lastname: Morifuku
authors.affiliation: Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Enokida
mesh_terms: D028202:Abies / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D007564:Japan; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014197:Trees / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D027081:DNA Gyrase