title: Application of an ELISA-type screen printed electrode-based potentiometric assay to the detection of Cryptosporidium parvum oocysts.
abstract: We report a novel electrochemical method for the rapid detection of the parasitic protozoan, Cryptosporidium parvum. An antibody-based capture format was transferred onto screen-printed electrodes and the presence of horseradish peroxidase-labelled antibodies binding to the oocysts was potentiometrically detected. This method allowed the detection of 5 × 10(2)Cryptosporidium oocysts per mL in 60 min.
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia; Environmental Biotechnology CRC, Australian Technology Park, Eveleigh, NSW 2006, Australia. Electronic address: Olivier.Laczka@uts.edu.au.
authors.forename: Olivier
authors.initials: O
authors.lastname: Laczka
authors.forename: Lucy
authors.initials: L
authors.lastname: Skillman
authors.forename: William G
authors.initials: WG
authors.lastname: Ditcham
authors.forename: Brenton
authors.initials: B
authors.lastname: Hamdorf
authors.forename: Danny K Y
authors.initials: DK
authors.lastname: Wong
authors.forename: Peter
authors.initials: P
authors.lastname: Bergquist
authors.forename: Anwar
authors.initials: A
authors.lastname: Sunna
mesh_terms: D000906:Antibodies / Q000737:chemistry; D016785:Cryptosporidium parvum / Q000235:genetics / Q000302:isolation & purification*; D004566:Electrodes; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D033661:Oocysts; D011199:Potentiometry / Q000379:methods*
chemical_list: D000906:Antibodies
keywords: Antibodies; Cryptosporidium oocysts; Potentiometry; Screen-printed electrodes