title: Down-regulation of miR-30c promotes the invasion of non-small cell lung cancer by targeting MTA1.
abstract: BACKGROUND
  The connection between microRNA expression and lung cancer development has been identified in recent literature. However, the mechanism of microRNA has been poorly elucidated in non-small-cell lung cancer (NSCLC).
  
  
  RESULTS
  Comparing with adjacent tissues (n=75), miR-30c has a lower expression in lung cancer specimens (n=75). The knockdown of miR-30c enhanced the invasion of A549 cells; meanwhile, the overexpression of miR-30c could reverse the effect of the knockdown of miR-30c in vitro. A luciferase assay revealed that miR-30c was directly bound to the 3'-untranslated regions (3'-UTR) of MTA1. QRT-PCR and western blot shows MTA1 was up-regulated in mRNA and protein levels. The effect taken on the invasion of NSCLC by overexpression of MTA1 works the same as down-regulated miR-30c.
  
  
  CONCLUSIONS
  miR-30c may play a pivotal role in controlling lung cancer invasion through regulating MTA1in NSCLC.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xia
authors.forename: Qiyou
authors.initials: Q
authors.lastname: Chen
authors.forename: Zhaopeng
authors.initials: Z
authors.lastname: Zhong
authors.forename: Caihua
authors.initials: C
authors.lastname: Xu
authors.forename: Chen
authors.initials: C
authors.lastname: Wu
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Yijiang
authors.initials: Y
authors.lastname: Chen
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D015536:Down-Regulation; D006655:Histone Deacetylases / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000503:physiopathology; D016133:Polymerase Chain Reaction; D012097:Repressor Proteins / Q000378:metabolism*; D015534:Trans-Activators
chemical_list: C546086:MIRN30b microRNA, human; D035683:MicroRNAs; C089320:MTA1 protein, human; D012097:Repressor Proteins; D015534:Trans-Activators; D006655:Histone Deacetylases