title: The in vitro activity of products formed from exposure of chlorpromazine to a 266 nm laser beam against species of mycobacteria of human interest.
abstract: Chlorpromazine (CPZ) was exposed to a 266 nm laser beam for different periods of time ranging from minutes to 24 h. At intervals, the products from irradiation were evaluated by thin-layer chromatography (TLC) and evaluated for their activity against mycobacteria of human interest (Mycobacterium tuberculosis, M. avium, M. intracellulare and their corresponding reference strains or clinical isolates). With the exception of the M. avium 47/07 clinical strain, the products produced from the irradiation of CPZ for 4 h had greater activity against M. intracellulare ATCC, M. avium ATCC, H37Rv and the Multidrug-resistant tuberculosis (MDR-TB) strains as opposed to that produced by the unirradiated control. The level of products from the 4-h exposure of CPZ remained the same throughout the next 20 h of irradiation. Of significant note is that the irradiation products of CPZ had lower in vitro cytotoxicity against human cells, suggesting that this approach may be useful for the development of compounds more bioactive than the parental species.
authors.affiliation: Center from Malaria and Other Tropical Diseases (CMDT), Institute of Hygiene and Tropical Medicine (IHMT), New University of Lisbon, Lisbon, Portugal.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Armada
authors.forename: Tatiana
authors.initials: T
authors.lastname: Alexandru
authors.forename: Diana
authors.initials: D
authors.lastname: Machado
authors.forename: Balazs
authors.initials: B
authors.lastname: Danko
authors.forename: Attila
authors.initials: A
authors.lastname: Hunyadi
authors.forename: Andra
authors.initials: A
authors.lastname: Dinache
authors.forename: Viorel
authors.initials: V
authors.lastname: Nastasa
authors.forename: Mihai
authors.initials: M
authors.lastname: Boni
authors.forename: Jorge
authors.initials: J
authors.lastname: Ramos
authors.forename: Miguel
authors.initials: M
authors.lastname: Viveiros
authors.forename: Joseph
authors.initials: J
authors.lastname: Molnar
authors.forename: Mihail Lucian
authors.initials: ML
authors.lastname: Pascu
authors.forename: Leonard
authors.initials: L
authors.lastname: Amaral
mesh_terms: D000995:Antitubercular Agents / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D002460:Cell Line; D002746:Chlorpromazine / Q000737:chemistry* / Q000528:radiation effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D007834:Lasers; D008826:Microbial Sensitivity Tests; D009161:Mycobacterium / Q000187:drug effects*
chemical_list: D000995:Antitubercular Agents; D002746:Chlorpromazine
keywords: 266nm; Laser beam; chlorpromazine; cytotoxicity of products; laser beam; mycobacteria