title: Selection and characterization of single-chain recombinant antibodies against spring viraemia of carp virus from mouse phage display library.
abstract: Antibody-displaying phage library was selected after three rounds of panning against spring viraemia of carp virus (SVCV) by phage display technology. Eight positive clones which could produce soluble single-chain fragment variable (scFv) antibody induced by isopropyl-beta-d-thiogalactopyranoside (IPTG) were obtained. Dot blot results showed that the eight scFv antibodies could recognize SVCV. The soluble scFv antibodies showed a molecular weight 29 kD by Western blot. All scFv antibodies could recognize SVCV proteins specifically without cross-reaction with other virus proteins by ELISA. Indirect immunofluorescence results showed that all of these scFv antibodies reacted positively with virus in the SVCV-infected cells. These scFv antibodies will be useful tools to establish immunological detection methods for SVCV.
authors.affiliation: The Key Laboratory of Aquatic Animal Diseases, Shenzhen Exit & Entry Inspection and Quarantine Bureau, Shenzhen 518001, PR China; College of Fisheries, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Xiaocong
authors.initials: X
authors.lastname: Zheng
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhang
authors.forename: Heping
authors.initials: H
authors.lastname: Dai
authors.forename: Qunyi
authors.initials: Q
authors.lastname: Hua
authors.forename: Xiujie
authors.initials: X
authors.lastname: Shi
authors.forename: Wensheng
authors.initials: W
authors.lastname: Lan
authors.forename: Peng
authors.initials: P
authors.lastname: Jia
authors.forename: Li
authors.initials: L
authors.lastname: Yuan
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification*; D015153:Blotting, Western; D002347:Carps; D061505:Cell Surface Display Techniques; D051379:Mice; D008970:Molecular Weight; D057127:Single-Chain Antibodies / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification*; D018116:Vesiculovirus / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D057127:Single-Chain Antibodies
keywords: Phage display; Single-chain fragment variable; Spring viraemia of carp virus