title: Simultaneous determination of three purines in Alysicarpus vaginalis (L.) DC. by hollow fiber-based liquid-phase microextraction combined with high-performance liquid chromatography.
abstract: In this paper, a three-phase hollow fiber liquid-phase microextraction (HF-LPME) method combined with high-performance liquid chromatography (HPLC) was developed for the determination of hypoxanthine (HX), xanthine (Xan) and adenine (A) and then for the first time successfully applied to the analysis of HX, Xan and A in Alysicarpus vaginalis (L.) DC. medicinal materials. Different factors affecting the HF-LPME procedure were investigated and optimized. Under optimal extraction conditions (1-octanol as organic solvent, pH of the donor and acceptor phase 10.0 and 3.5, respectively, extraction time 40 min, stirring rate 800 rpm and salt addition 10%, w/v), HX, Xan and A could be determined within the test ranges with a good correlation coefficient (r(2) > 0.9992). The limit of detection for HX, Xan and A was 153, 173 and 97 ng/mL, respectively, and the intra- and inter-day relative standard deviations were no more than 9.8%. The content of HX, Xan and A in Alysicarpus vaginalis (L.) DC. medicinal materials was 120.40, 18.37 and 62.75 µg/g, respectively. This procedure afforded a convenient, sensitive, accurate and inexpensive method with a high extraction efficiency for determination of HX, Xan and A.
authors.affiliation: Department of Pharmaceutics, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, People's Republic of China.
authors.forename: Hongjiao
authors.initials: H
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Lei
authors.forename: Xiao
authors.initials: X
authors.lastname: Liang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Jiang
authors.forename: Xingjie
authors.initials: X
authors.lastname: Guo
mesh_terms: D020003:1-Octanol; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007887:Fabaceae / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D059627:Liquid Phase Microextraction / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry; D011687:Purines / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012965:Sodium Chloride
chemical_list: D010936:Plant Extracts; D011687:Purines; D012965:Sodium Chloride; D020003:1-Octanol
keywords: Alysicarpus vaginalis (L.) DC; adenine; hollow fiber liquid-phase microextraction; hypoxanthine; xanthine