title: miR-26a suppresses tumor growth and metastasis by targeting FGF9 in gastric cancer.
abstract: The role of miR-26a in cancer cells seemed controversial in previous studies. Until now, the role of miR-26a in gastric cancer remains undefined. In this study, we found that miR-26a was strongly downregulated in gastric cancer (GC) tissues and cell lines, and its expression levels were associated with lymph node metastasis and clinical stage, as well as overall survival and replase-free survival of GC. We also found that ectopic expression of miR-26a inhibited GC cell proliferation and GC metastasis in vitro and in vivo. We further identified a novel mechanism of miR-26a to suppress GC growth and metastasis. FGF9 was proved to be a direct target of miR-26a, using luciferase assay and western blot. FGF9 overexpression in miR-26a-expressing cells could rescue invasion and growth defects of miR-26a. In addition, miR-26a expression inversely correlated with FGF9 protein levels in GC. Taken together, our data suggest that miR-26a functions as a tumor suppressor in GC development and progression, and holds promise as a prognostic biomarker and potential therapeutic target for GC.
authors.affiliation: Cancer Hospital and Cancer Research Institute, Guangzhou Medical University, Guangzhou, China ; Cancer Research Institute, University of South China, Hengyang, China.
authors.forename: Min
authors.initials: M
authors.lastname: Deng
authors.forename: Hai-lin
authors.initials: HL
authors.lastname: Tang
authors.forename: Xi-hong
authors.initials: XH
authors.lastname: Lu
authors.forename: Mei-yuan
authors.initials: MY
authors.lastname: Liu
authors.forename: Xiao-min
authors.initials: XM
authors.lastname: Lu
authors.forename: Yi-xue
authors.initials: YX
authors.lastname: Gu
authors.forename: Ji-fang
authors.initials: JF
authors.lastname: Liu
authors.forename: Zhi-min
authors.initials: ZM
authors.lastname: He
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D051525:Fibroblast Growth Factor 9 / Q000096:biosynthesis* / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000628:therapy
chemical_list: D014408:Biomarkers, Tumor; D051525:Fibroblast Growth Factor 9; D035683:MicroRNAs; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; C496459:FGF9 protein, human; C528163:MIRN26 microRNA, human