title: Maternal acrylamide treatment reduces ovarian follicle number in newborn guinea pig offspring.
abstract: Acrylamide is an industrial chemical which has toxic effects on reproduction. In this study, we investigated whether acrylamide administered prenatally can induce follicular atresia in the newborn guinea pig ovary. Another aim was to describe the localization of vimentin filaments and determine their participation in atresia. After prenatal acrylamide treatment, the pool of primordial and primary follicles was significantly reduced. The number of caspase 3 and TUNEL positive oocytes increased compared to the control group. There were no differences in Lamp1 (autophagy marker) staining. A vimentin immunosignal was present in the granulosa cells of primordial, primary and secondary follicles. Interestingly, in contrast to the control group, the oocytes from all follicles in the ACR-treated females were negative for vimentin. These data suggest that prenatal exposure to acrylamide reduced the number of ovarian follicles by inducing follicular atresia mediated by oocyte apoptosis. Acrylamide-induced apoptosis may be associated with destruction of vimentin filaments.
authors.affiliation: Department of Anatomy and Anthropology, Maria Curie-Sklodowska University, Akademicka St. 19, 20-033 Lublin, Poland. Electronic address: monhul@o2.pl.
authors.forename: Monika
authors.initials: M
authors.lastname: Hułas-Stasiak
authors.forename: Piotr
authors.initials: P
authors.lastname: Dobrowolski
authors.forename: Ewa
authors.initials: E
authors.lastname: Tomaszewska
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Kostro
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000818:Animals; D000831:Animals, Newborn; D005260:Female; D005496:Follicular Atresia / Q000378:metabolism; D006168:Guinea Pigs; D008431:Maternal-Fetal Exchange; D006080:Ovarian Follicle / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D014746:Vimentin / Q000378:metabolism
chemical_list: D014746:Vimentin; D020106:Acrylamide
keywords: Acrylamide; Apoptosis; Atresia; Guinea pig; Ovarian follicles; Vimentin filaments