title: Isothermal strand-displacement polymerase reaction for visual detection of the Southeast Asian-type deletion of α-thalassemia.
abstract: A rapid and reliable screening test for thalassemia carrier couples is the most effective strategy to decrease the risk of conceiving fetuses with severe thalassemia. We present an approach based on the isothermal strand-displacement polymerase reaction and the use of a lateral flow strip for the visual detection of an α-thalassemia Southeast Asian-type deletion. This assay was used to evaluate 86 clinical samples (72 cases of Southeast Asian-type deletions and 14 cases of other types of thalassemia), and the results obtained were 100% consistent with those obtained using conventional gap-PCR. The approach thus provides a simple, sensitive, rapid, and cost-effective method for the diagnosis of thalassemia genotypes.
authors.affiliation: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
authors.forename: Luxin
authors.initials: L
authors.lastname: Yu
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Puchang
authors.initials: P
authors.lastname: Lie
authors.forename: Yunhua
authors.initials: Y
authors.lastname: Liu
authors.forename: Lingwen
authors.initials: L
authors.lastname: Zeng
mesh_terms: D016022:Case-Control Studies; D005820:Genetic Testing; D006801:Humans; D025202:Molecular Diagnostic Techniques; D021141:Nucleic Acid Amplification Techniques; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D017384:Sequence Deletion; D055542:alpha-Globins / Q000235:genetics; D017085:alpha-Thalassemia / Q000175:diagnosis* / Q000235:genetics
chemical_list: D011933:Reagent Kits, Diagnostic; D055542:alpha-Globins