title: [Study on inhibitory effect of lycium pigment on lipopolysaccharide-induced uveitis in rats and its mechanism].
abstract: OBJECTIVE
  To investigate the inhibitory effect of lycium pigment on lipopolysaccharide (LPS)-induced uveitis in rats and its mechanism.
  
  
  METHODS
  The rat uveitis model was established by 30-day oral administration of lycium pigment (50, 100, 200 mg x kg(-1)) and footpad injection of LPS. Ocular tissues were collected for a histopathological inspection. The protein, nitric oxide and ADMA in aqueous humor, level of inducible nitric oxide synthase (iNOS) in retina, activities of serum total antioxidant capacity (T-AOC), superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), and content of malondialdehyde (MDA) were determined by using Western blot, ELISA and biochemical methods.
  
  
  RESULTS
  According to the pathological study, lycium pigment (50, 100, 200 mg x kg(-1)) could notably reduce the inflammatory cell infiltration around corpus ciliare matrix of uveitis rats, and the concentration of protein and nitric oxide, and increased ADMA in aqueous humor. Lycium pigment (100, 200 mg x kg(-1)) could significantly inhibit the expression of iNOS in ocular tissues. In addition, lycium pigment (100, 200 mg x kg(-1)) also decrease the activities of serum T-AOC, SOD, GSH-PX, and the content of lipid peroxide MDA.
  
  
  CONCLUSIONS
  Lycium pigment has the inhibitory effect on LPS-induced uveitis in rats. Its mechanism is related to the regulation of nitric oxide/ADMA pathway and the improvement of oxidation resistance.
authors.affiliation: School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China. 7253918@qq.com
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Zhao
authors.forename: Shuang
authors.initials: S
authors.lastname: Bai
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
authors.forename: Ping
authors.initials: P
authors.lastname: Zheng
authors.forename: Gui-Dong
authors.initials: GD
authors.lastname: Dai
mesh_terms: D000818:Animals; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008070:Lipopolysaccharides / Q000009:adverse effects*; D032305:Lycium / Q000737:chemistry*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D010860:Pigments, Biological / Q000008:administration & dosage*; D010936:Plant Extracts / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014605:Uveitis / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*
chemical_list: D008070:Lipopolysaccharides; D010860:Pigments, Biological; D010936:Plant Extracts; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D052247:Nitric Oxide Synthase Type II; D013482:Superoxide Dismutase