title: Antioxidant and antimicrobial properties of phenolic rich fraction of Seabuckthorn (Hippophae rhamnoides L.) leaves in vitro.
abstract: Phenolic rich fraction (PRF) from Seabuckthorn leaves was prepared by sequential fractionation. Total phenolic content of PRF estimated as gallic acid equivalent was found to be 319.33±7.02 mg/g of PRF. Its major constituents gallic acid, rutin, quercetin-3-galactoside, quercetin-3-glucoside, myricetin, quercetin, kaempferol and isorhamnetin, were found in the range of 1.551-196.89 mg/g of PRF as determined by RP-HPLC. Antioxidant activity of PRF evaluated using 2,2-diphenyl-2-picrylhydrazyl, superoxide and nitric oxide scavenging assays. Reducing power of PRF increased with increasing amount of PRF; the equation of reducing power (y) and amount of PRF (x) was y=8.004x (r(2)=0.99), indicating that reducing ability correlated well with amount of PRF. Antibacterial activity of PRF, tested against certain medically important bacterial species showed growth inhibiting effect against Escherichia coli, Salmonella typhi, Shigella dysenteriae, Streptococcus pneumoniae and Staphylococcus aureus. In conclusion, PRF has potent antioxidant and broad spectrum antibacterial properties.
authors.affiliation: Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi 110 054, India. msyogendra@gmail.com
authors.forename: M S
authors.initials: MS
authors.lastname: Yogendra Kumar
authors.forename: R J
authors.initials: RJ
authors.lastname: Tirpude
authors.forename: D T
authors.initials: DT
authors.lastname: Maheshwari
authors.forename: Anju
authors.initials: A
authors.lastname: Bansal
authors.forename: Ksipra
authors.initials: K
authors.lastname: Misra
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D031281:Hippophae / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts
keywords: Antimicrobial activity; Antioxidant; HPLC; Phenol; Seabuckthorn