title: Reactive oxygen species mediate dopamine-induced signaling in renal proximal tubule cells.
abstract: Intrarenally-produced dopamine (DA) induces a large increase in urinary sodium excretion mainly due to the inhibition of tubular sodium reabsorption. We aimed to study the participation of reactive oxygen species (ROS) in DA signaling pathway in proximal tubule cells. Our results show that DA increased ROS production in OK cells and indicate the mitochondria as the main source of ROS. DA also increased ERK1/2, superoxide dismutase (SOD) and transcription factor κB (NF-κB) activity. These findings suggest that DA generates mitochondria-derived ROS that activate ERK1/2 and subsequently NF-κB and SOD activity at concentrations that exert a physiological regulation of renal function.
authors.affiliation: Institute for Biomedical Research (INBIOMED), Department of Biochemistry, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina; Pharmacology Unit, School of Dentistry, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: Andrea B
authors.initials: AB
authors.lastname: Acquier
authors.forename: Mercedes
authors.initials: M
authors.lastname: Mori Sequeiros García
authors.forename: Alejandra B
authors.initials: AB
authors.lastname: Gorostizaga
authors.forename: Cristina
authors.initials: C
authors.lastname: Paz
authors.forename: Carlos F
authors.initials: CF
authors.lastname: Mendez
mesh_terms: D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004298:Dopamine / Q000502:physiology*; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D008928:Mitochondria / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D009893:Opossums; D010766:Phosphorylation; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D017382:Reactive Oxygen Species; D002374:Catalase; D001094:Arachidonate 5-Lipoxygenase; D051546:Cyclooxygenase 2; D013482:Superoxide Dismutase; D004298:Dopamine
keywords: DA; Dopamine; ERK1/2; Kidney; MAO; NF-κB; OK; Proximal tubule; ROS; SOD; dopamine; extracellular signal-regulated kinases 1/2; monoaminoxidase; nuclear transcription factor κB; opossum kidney cells; reactive oxygen species; superoxide dismutase