title: Light-induced reactivation of O2-tolerant membrane-bound [Ni-Fe] hydrogenase from the hyperthermophilic bacterium Aquifex aeolicus under turnover conditions.
abstract: We report the effect of UV-Vis light on the membrane-bound [Ni-Fe] hydrogenase from Aquifex aeolicus under turnover conditions. Using electrochemistry, we show a potential dependent light sensitivity and propose that a light-induced structural change of the [Ni-Fe] active site is related to an enhanced reactivation of the hydrogenase under illumination at high potentials.
authors.affiliation: Bioénergétique et Ingénierie des Protéines, Institut de Microbiologie de la Méditerranée - CNRS-AMU, 31 Chemin Aiguier, 13009 Marseille, France. lojou@imm.cnrs.fr.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Ciaccafava
authors.forename: Cyrille
authors.initials: C
authors.lastname: Hamon
authors.forename: Pascale
authors.initials: P
authors.lastname: Infossi
authors.forename: Valérie
authors.initials: V
authors.lastname: Marchi
authors.forename: Marie-Thérèse
authors.initials: MT
authors.lastname: Giudici-Orticoni
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Lojou
mesh_terms: D001419:Bacteria / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D004789:Enzyme Activation; D006358:Hot Temperature; D006864:Hydrogenase / Q000737:chemistry* / Q000378:metabolism*; D007700:Kinetics; D008027:Light; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D010100:Oxygen / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; C043296:nickel-iron hydrogenase; D006864:Hydrogenase; D010100:Oxygen