title: Study of microbial community and biodegradation efficiency for single- and two-phase anaerobic co-digestion of brown water and food waste.
abstract: The objective of this work was to study the microbial community and reactor performance for the anaerobic co-digestion of brown water and food waste in single- and two-phase continuously stirred tank reactors (CSTRs). Bacterial and archaeal communities were analyzed after 150 days of reactor operation. As compared to single-phase CSTR, methane production in two-phase CSTR was found to be 23% higher. This was likely due to greater extent of solubilization and acidification observed in the latter. These findings could be attributed to the predominance of Firmicutes and greater bacterial diversity in two-phase CSTR, and the lack of Firmicutes in single-phase CSTR. Methanosaeta was predominant in both CSTRs and this correlated to low levels of acetate in their effluent. Insights gained from this study would enhance the understanding of microorganisms involved in co-digestion of brown water and food waste as well as the complex biochemical interactions promoting digester stability and performance.
authors.affiliation: Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore; Division of Environmental and Water Resources, School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore. Electronic address: jwlim3@e.ntu.edu.sg.
authors.forename: J W
authors.initials: JW
authors.lastname: Lim
authors.affiliation: Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore. Electronic address: clchen@ntu.edu.sg.
authors.forename: C-L
authors.initials: CL
authors.lastname: Chen
authors.affiliation: Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore. Electronic address: ijrho@ntu.edu.sg.
authors.forename: I J R
authors.initials: IJR
authors.lastname: Ho
authors.affiliation: Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore; Division of Environmental and Water Resources, School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore. Electronic address: jywang@ntu.edu.sg.
authors.forename: J-Y
authors.initials: JY
authors.lastname: Wang
mesh_terms: D000693:Anaerobiosis; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001673:Biodegradation, Environmental; D019149:Bioreactors; D017931:DNA Primers; D005502:Food; D017404:In Situ Hybridization, Fluorescence; D010802:Phylogeny; D062065:Wastewater
chemical_list: D017931:DNA Primers; D062065:Wastewater
keywords: Anaerobic co-digestion; Brown water; Food waste; Microbial community structure; Single- and two-phase CSTR