title: [Expression of angiopoietin-2 and vascular endothelial growth factor in human colon cancer].
abstract: OBJECTIVE
  To investigate the association of the expressions of angiopoietin-2 (Ang-2), vascular endothelial growth factor (VEGF) in colorectal cancer tissues with Dukes' clinicopathological features.
  
  
  METHODS
  Ang-2 and Tie-2 mRNA expressions were detected in colorectal cancer tissues, adjacent tissues, and normal tissues by real time-PCR. Quantikine immunoassays were used to measure the protein expressions of Ang-2 and VEGF in the tissues and serum samples.
  
  
  RESULTS
  Ang-2 and Tie-2 levels were significantly higher in the serum of the patients than in the normal tissues (P<0.05), and their expressions were strongly correlated (r=0.879, P=0.000). Tumor tissue Ang-2 and VEGF levels were significantly higher than their levels in the adjacent and normal tissues (P<0.05). In colorectal cancer patients, the peripheral blood level of Ang-2 was significantly higher than that in healthy control subjects, and comparable with that in mesenteric blood (P>0.05). In Dukes' stage C and D patients, serum Ang-2 and VEGF levels were significantly higher than those in patients in Dukes' stage A and B (P<0.05).
  
  
  CONCLUSIONS
  Ang-2 and VEGF over expressions may play an important role in the occurrence and progression of colorectal cancer.
authors.affiliation: Department of Gastroenterology, Shanghai Sixth People's Hospital South Campus, Shanghai Jiao Tong University, Shanghai 201499, China. E-mail: ruihuawang@vip.sina.com.
authors.forename: Ruihua
authors.initials: R
authors.lastname: Wang
authors.forename: Jianguo
authors.initials: J
authors.lastname: Xie
authors.forename: Hua
authors.initials: H
authors.lastname: Chen
authors.forename: Tingting
authors.initials: T
authors.lastname: Ren
authors.forename: Yicui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D042702:Angiopoietin-2 / Q000097:blood / Q000378:metabolism*; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D042461:Vascular Endothelial Growth Factor A / Q000097:blood / Q000378:metabolism*
chemical_list: C586513:ANGPT2 protein, human; D042702:Angiopoietin-2; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A