title: Efficient, specific, developmentally appropriate cre-mediated recombination in anterior pituitary gonadotropes and thyrotropes.
abstract: Tissue-specific expression of cre recombinase is a well-established genetic tool to analyze gene function, and it is limited only by the efficiency and specificity of available cre mouse strains. Here, we report the generation of a transgenic line containing a cre cassette with codon usage optimized for mammalian cells (iCre) under the control of a mouse glycoprotein hormone α-subunit (αGSU) regulatory sequences in a bacterial artificial chromosome genomic clone. Initial analysis of this transgenic line, Tg(αGSU-iCre), with cre reporter strains reveals onset of cre activity in the differentiating cells of the developing anterior pituitary gland at embryonic day 12.5, with a pattern characteristic of endogenous αGSU. In adult mice, αGSU-iCre was active in the anterior lobe of the pituitary gland and in the cells that produce αGSU (gonadotropes and thyrotropes) with high penetrance. Little or no activity was observed in other tissues, including skeletal and cardiac muscle, brain, kidney, lungs, testis, ovary, and liver. This αGSU-iCre line is suitable for efficient, specific, and developmentally regulated deletion of floxed alleles in anterior pituitary gonadotropes and thyrotropes.
authors.affiliation: Department of Human Genetics, University of Michigan, Ann Arbor, Michigan.
authors.forename: María Inés
authors.initials: MI
authors.lastname: Pérez-Millán
authors.forename: Michael G
authors.initials: MG
authors.lastname: Zeidler
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Saunders
authors.forename: Sally A
authors.initials: SA
authors.lastname: Camper
authors.forename: Shannon W
authors.initials: SW
authors.lastname: Davis
mesh_terms: D000483:Alleles; D000818:Animals; D022202:Chromosomes, Artificial, Bacterial; D003001:Cloning, Molecular; D004622:Embryo, Mammalian; D005260:Female; D005838:Genotype; D015292:Glycoprotein Hormones, alpha Subunit / Q000235:genetics* / Q000378:metabolism; D052681:Gonadotrophs / Q000378:metabolism*; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009928:Organ Specificity; D011995:Recombination, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D052684:Thyrotrophs / Q000378:metabolism*
chemical_list: D015292:Glycoprotein Hormones, alpha Subunit; C045073:Cre recombinase; D019426:Integrases
keywords: BAC; Cga; alpha-GSU; chorionic gonadotropin alpha subunit; site specific recombination; transgenic mouse