title: Expression and localization of aging markers in lacrimal gland of chronic graft-versus-host disease.
abstract: Aging is commonly defined as the accumulation of diverse deleterious changes in cells and tissues with advancing age. To investigate whether aging changes are involved in the lacrimal glands of chronic graft-versus-host disease (cGVHD) model mice, we obtained the specimens from cGVHD model mice, untreated aged and young mice, and examined by histopathology, and immunoblotting. Oxidative stress markers, 8-OHdG, 4-HNE, and hexonoyl lesion (HEL), and other aging markers, p16 and p38, were used to assess the samples. The infiltrating mononuclear cells and endothelia of capillaries in the cGVHD and aged mice expressed the oxidative stress markers and other aging markers, but not in the young mice. Histological changes and the expression of aging markers in the samples from cGVHD mice exhibited similar features to those in aging mice. These results suggest that changes that typically appear with advanced age occur earlier in the lives of mice with lacrimal gland cGVHD.
authors.affiliation: Department of Ophthalmology, Keio University, School of Medicine.
authors.forename: Masataka
authors.initials: M
authors.lastname: Kawai
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ogawa
authors.forename: Shigeto
authors.initials: S
authors.lastname: Shimmura
authors.forename: Shigeki
authors.initials: S
authors.lastname: Ohta
authors.forename: Takanori
authors.initials: T
authors.lastname: Suzuki
authors.forename: Naoshi
authors.initials: N
authors.lastname: Kawamura
authors.forename: Masataka
authors.initials: M
authors.lastname: Kuwana
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kawakami
authors.forename: Kazuo
authors.initials: K
authors.lastname: Tsubota
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000375:Aging / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers; D002908:Chronic Disease; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D006086:Graft vs Host Disease / Q000209:etiology / Q000378:metabolism*; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects; D007765:Lacrimal Apparatus / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D015227:Lipid Peroxidation; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D015415:Biomarkers; D017382:Reactive Oxygen Species; D000080242:8-Hydroxy-2'-Deoxyguanosine; D048051:p38 Mitogen-Activated Protein Kinases; D003849:Deoxyguanosine