title: Peptide interaction with and insertion into membranes.
abstract: Natural and synthetic membrane active peptides as well as fragments from membrane proteins interact with membranes. In several cases, such interactions cause the insertion of the peptides to the membrane and their assembly within the lipid bilayer. Here we present spectroscopic approaches utilizing NBD and rhodamine fluorescently labeled peptides to measure peptide-membrane interaction and peptide-peptide interaction within the membrane. The usage of the physical properties of NBD and rhodamine in solution and in membranes provides useful information on the interplay between peptides and lipids.
authors.affiliation: Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Ron
authors.initials: R
authors.lastname: Saar-Dover
authors.forename: Avraham
authors.initials: A
authors.lastname: Ashkenazi
authors.forename: Yechiel
authors.initials: Y
authors.lastname: Shai
mesh_terms: D019286:Endopeptidase K / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D008566:Membranes / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding; D059748:Proteolysis; D013194:Staining and Labeling / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D008565:Membrane Proteins; D010455:Peptides; D019286:Endopeptidase K