title: Impact of bacteria and bacterial components on osteogenic and adipogenic differentiation of adipose-derived mesenchymal stem cells.
abstract: Adult mesenchymal stem cells (MSC) are present in several tissues, e.g. bone marrow, heart muscle, brain and subcutaneous adipose tissue. In invasive infections MSC get in contact with bacteria and bacterial components. Not much is known about how bacterial pathogens interact with MSC and how contact to bacteria influences MSC viability and differentiation potential. In this study we investigated the impact of three different wound infection relevant bacteria, Escherichia coli, Staphylococcus aureus, and Streptococcus pyogenes, and the cell wall components lipopolysaccharide (LPS; Gram-negative bacteria) and lipoteichoic acid (LTA; Gram-positive bacteria) on viability, proliferation, and osteogenic as well as adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells (adMSC). We show that all three tested species were able to attach to and internalize into adMSC. The heat-inactivated Gram-negative E. coli as well as LPS were able to induce proliferation and osteogenic differentiation but reduce adipogenic differentiation of adMSC. Conspicuously, the heat-inactivated Gram-positive species showed the same effects on proliferation and adipogenic differentiation, while its cell wall component LTA exhibited no significant impact on adMSC. Therefore, our data demonstrate that osteogenic and adipogenic differentiation of adMSC is influenced in an oppositional fashion by bacterial antigens and that MSC-governed regeneration is not necessarily reduced under infectious conditions.
authors.affiliation: Institute for Medical Microbiology, Virology, and Hygiene, Rostock University Medical Center, Schillingallee 70, D-18057 Rostock, Germany. Electronic address: tomas.fiedler@med.uni-rostock.de.
authors.forename: Tomas
authors.initials: T
authors.lastname: Fiedler
authors.forename: Achim
authors.initials: A
authors.lastname: Salamon
authors.forename: Stefanie
authors.initials: S
authors.lastname: Adam
authors.forename: Nicole
authors.initials: N
authors.lastname: Herzmann
authors.forename: Jan
authors.initials: J
authors.lastname: Taubenheim
authors.forename: Kirsten
authors.initials: K
authors.lastname: Peters
mesh_terms: D050156:Adipogenesis / Q000187:drug effects*; D000273:Adipose Tissue / Q000166:cytology; D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000378:metabolism; D001419:Bacteria / Q000737:chemistry / Q000378:metabolism; D001422:Bacterial Adhesion; D018407:Bacterial Physiological Phenomena; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006358:Hot Temperature; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000382:microbiology; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000187:drug effects*; D060888:Real-Time Polymerase Chain Reaction; D013682:Teichoic Acids / Q000494:pharmacology
chemical_list: D000276:Adjuvants, Immunologic; D008070:Lipopolysaccharides; D013682:Teichoic Acids; C009900:lipoteichoic acid; D000469:Alkaline Phosphatase
keywords: Endotoxin; Escherichia coli; LPS; LTA; MSC; Staphylococcus aureus; Streptococcus pyogenes; adMSC