title: [LC-MS quantification and pharmacokinetics of the multi-constituents of Huangqin Tang in rat plasma after different single oral doses].
abstract: The current study aims to investigate the pharmacokinetic properties of Huangqin Tang on different oral doses. An LC-MS method for simultaneous determination of flavonoids and terpenoids in rat plasma was developed and validated. Plasma samples were treated with hydrochloric acid (containing 1% ascorbic acid), precipitated with acetonitrile, separated on a Zorbax SB-C18 column, detected by single quadruple mass spectrometry with an electrospray ionization interface, and quantified using selected ion monitoring mode. All pharmacokinetic parameters were processed by non-compartmental analysis using WinNonlin software. The results of specificity, linearity, intra-day and inter-day precisions, accuracy, and stability for LC-MS assay were suitable for the quantification of paeoniflorin, baicalin, wogonoside, baicalein, wogonin, oroxylin A, glycyrrhizic acid and glycyrrhetinic acid in rat plasma. The concentration-time profiles of baicalin, wogonoside, baicalein, wogonin, oroxylin A and glycyrrhizic acid showed double-peak phenomenon after Huangqin Tang was orally administered at 40 g x kg(-1) dose; all eight constituents in rat plasma showed good dose-exposure relationship within the dosage of 10-40 g x kg(-1); although plasma concentrations were different, the flavonoids with the same backbone showed the similar fate in the body with the corresponding dosage. In conclusion, the LC-MS assay was successfully applied for the pharmacokinetic study of multi-constituents of Huangqin Tang with different doses. Additionally, these constituents demonstrated good pharmacokinetic properties in the body.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Wang
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Wang
authors.forename: Ri-Xin
authors.initials: RX
authors.lastname: Liang
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.forename: Hui-Hui
authors.initials: HH
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Zhong-Ming
authors.initials: ZM
authors.lastname: Zhou
authors.forename: Wei-Peng
authors.initials: WP
authors.lastname: Yang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002853:Chromatography, Liquid; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry*; D044950:Flavanones / Q000097:blood* / Q000493:pharmacokinetics; D005419:Flavonoids / Q000097:blood / Q000493:pharmacokinetics; D005960:Glucosides / Q000097:blood / Q000493:pharmacokinetics; D006034:Glycyrrhetinic Acid / Q000097:blood / Q000493:pharmacokinetics; D019695:Glycyrrhizic Acid / Q000097:blood / Q000493:pharmacokinetics; D008297:Male; D039821:Monoterpenes / Q000097:blood / Q000493:pharmacokinetics; D053978:Pentacyclic Triterpenes / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D021241:Spectrometry, Mass, Electrospray Ionization; D006027:Glycosides
chemical_list: D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; D006034:Glycyrrhetinic Acid; D019695:Glycyrrhizic Acid; D039821:Monoterpenes; D053978:Pentacyclic Triterpenes; C439205:Huangqin-Tang; C015423:peoniflorin; D006027:Glycosides; C038044:baicalin; C006680:baicalein; C080669:5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one; C473995:wogonoside; C085514:wogonin