title: Predictors of the yield of mobilized peripheral blood CD34+ cells in HLA-matched sibling donor.
abstract: We examined the donor factors that may affect the yield of peripheral blood stem cell (PBSC) mobilized from healthy donors. Pre-apheresis PB-CD34(+) cell count was the only factor that correlated with PBSC yield. Leukocyte count (LC) and monocyte count (MC) correlated with PB-CD34(+) cell. Male gender and PB-CD34(+) cell count of at least 87.1/μL and 69.8/μL on day-4 and -5 of G-CSF were associated with the ability to harvest at least 5×10(6)/kg CD34(+) cells after one apheresis. We concluded that gender and PB-CD34(+) cell count are important predictors of PBSC yield. LC and MC may serve as surrogate markers for estimating the PB-CD34(+) cell count.
authors.affiliation: Cell Therapy Centre, Kuala Lumpur, Malaysia; Department of Medicine, Kuala Lumpur, Malaysia. Electronic address: sfadilah@ppukm.ukm.edu.my.
authors.forename: S A W
authors.initials: SA
authors.lastname: Fadilah
authors.forename: M I
authors.initials: MI
authors.lastname: Mohd-Razif
authors.forename: Z A Z
authors.initials: ZA
authors.lastname: Seery
authors.forename: T
authors.initials: T
authors.lastname: Nor-Rafeah
authors.forename: W J
authors.initials: WJ
authors.lastname: Wan-Fariza
authors.forename: A
authors.initials: A
authors.lastname: Habsah
authors.forename: C F
authors.initials: CF
authors.lastname: Leong
mesh_terms: D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000276:immunology*; D001781:Blood Component Removal / Q000379:methods*; D002648:Child; D005260:Female; D006680:HLA Antigens / Q000276:immunology*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation / Q000379:methods*; D012189:Retrospective Studies; D035781:Siblings; D014019:Tissue Donors; D055815:Young Adult
chemical_list: D018952:Antigens, CD34; D006680:HLA Antigens
keywords: Healthy donors; Peripheral blood CD34(+) cell count; Peripheral blood stem cell (PBSC) mobilization