title: Gene expression profile and functional analysis of Alzheimer's disease.
abstract: BACKGROUND
  Alzheimer's disease (AD) is a complex neurodegenerative disorder with largely unknown genetic mechanisms. Identifying altered neuronal gene expression in brain regions differentially affected by AD may provide the diagnostic or therapeutic targets of AD.
  
  
  METHODS
  The gene expression profile of AD was analyzed with bioinformatics. Function analysis was performed with Database for Annotation, Visualization and Integrated Discovery (DAVID), and TransFind was used to predict the possible transcriptional regulators in AD. Finally, connectivity map (cMap) database was used to explore small molecules targeted for AD.
  
  
  RESULTS
  The AD gene signatures associated with 6 different brain regions were identified. Functional analysis revealed that biological processes involved with metabolism, protein ubiquitination, and vasculature development were found dysregulated, and synaptic signaling pathways were found perturbated in AD. The WT1 was identified as an important transcriptional regulator in AD, and cMap database predicted that small molecules, such as histone deacetylase (HDAC) inhibitor, may be candidate drugs in the treatment of AD.
  
  
  CONCLUSIONS
  According to our in silico analysis, Wilms' tumor suppressor may play regulatory roles in AD development and progress. The HDAC inhibitor could possibly be used to treat AD.
authors.affiliation: 1Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.forename: Qiang
authors.initials: Q
authors.lastname: Guan
authors.forename: Zhi-Yu
authors.initials: ZY
authors.lastname: Nie
authors.forename: Ling-Jing
authors.initials: LJ
authors.lastname: Jin
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D015398:Signal Transduction; D013569:Synapses / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics* / Q000502:physiology
keywords: Alzheimer’s disease; connectivity map; functional analysis; gene expression profile