title: Preparation of fragment libraries for next-generation sequencing on the applied biosystems SOLiD platform.
abstract: The primary purpose of this protocol is to prepare genomic DNA libraries that can then be analyzed by massively parallel next-generation sequencing on the Applied Biosystems SOLiD platform. This protocol can be adapted to next-generation sequencing workflows to ultimately generate up to 1 billion 50 bp sequence tags from the ends of each of the DNA molecules in the library in a single next-generation sequencing run.
authors.affiliation: Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: syegnasu@jhmi.edu.
authors.forename: Srinivasan
authors.initials: S
authors.lastname: Yegnasubramanian
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000235:genetics; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods*; D016133:Polymerase Chain Reaction
chemical_list: D004247:DNA
keywords: Adaptor ligation; Amplification; DNA fragments; End-Repair of fragmented DNA; Integrated DNA Technologies (IDT); Invitrogen E-Gel system; Next-generation sequencing