title: Fast characterisation of cell-derived extracellular vesicles by nanoparticles tracking analysis, cryo-electron microscopy, and Raman tweezers microspectroscopy.
abstract: The joint use of 3 complementary techniques, namely, nanoparticle tracking analysis (NTA), cryo-electron microscopy (Cryo-EM) and Raman tweezers microspectroscopy (RTM), is proposed for a rapid characterisation of extracellular vesicles (EVs) of various origins. NTA is valuable for studying the size distribution and concentration, Cryo-EM is outstanding for the morphological characterisation, including observation of vesicle heterogeneity, while RTM provides the global chemical composition without using any exogenous label. The capabilities of this approach are evaluated on the example of cell-derived vesicles of Dictyostelium discoideum, a convenient general model for eukaryotic EVs. At least 2 separate species differing in chemical composition (relative amounts of DNA, lipids and proteins, presence of carotenoids) were found for each of the 2 physiological states of this non-pathogenic microorganism, that is, cell growth and starvation-induced aggregation. These findings demonstrate the specific potency of RTM. In addition, the first Raman spectra of human urinary exosomes are reported, presumably constituting the primary step towards Raman characterisation of EVs for the purpose of human diseases diagnoses.
authors.affiliation: Laboratoire Jean Perrin, FRE 3231 CNRS, Université Pierre et Marie Curie, Paris, France.
authors.forename: Irène
authors.initials: I
authors.lastname: Tatischeff
authors.forename: Eric
authors.initials: E
authors.lastname: Larquet
authors.forename: Juan M
authors.initials: JM
authors.lastname: Falcón-Pérez
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Turpin
authors.forename: Sergei G
authors.initials: SG
authors.lastname: Kruglik
keywords: Dictyostelium discoideum; Raman tweezers microspectroscopy; cryo-electron microscopy; extracellular vesicles; nanoparticle tracking analysis; urinary exosomes