title: Recombination-dependent oligomerization of human papillomavirus genomes upon transient DNA replication.
abstract: We describe the extensive and progressive oligomerization of human papillomavirus (HPV) genomes after transfection into the U2OS cell line. The HPV genomic oligomers are extrachromosomal concatemeric molecules containing the viral genome in a head-to-tail orientation. The process of oligomerization does not depend on the topology of the input DNA, and it does not require any other viral factors besides replication proteins E1 and E2. We provide evidence that oligomerization of the HPV18 and HPV11 genomes involves homologous recombination. We also demonstrate oligomerization of the HPV18 and HPV11 genomes in SiHa, HeLa, and C-33 A cell lines and provide examples of oligomeric HPV genomes in clinical samples obtained from HPV-infected patients.
authors.affiliation: University of Tartu, Institute of Technology, Tartu, Estonia.
authors.forename: Marit
authors.initials: M
authors.lastname: Orav
authors.forename: Liisi
authors.initials: L
authors.lastname: Henno
authors.forename: Helen
authors.initials: H
authors.lastname: Isok-Paas
authors.forename: Jelizaveta
authors.initials: J
authors.lastname: Geimanen
authors.forename: Mart
authors.initials: M
authors.lastname: Ustav
authors.forename: Ene
authors.initials: E
authors.lastname: Ustav
mesh_terms: D015139:Blotting, Southern; D001859:Bone Neoplasms / Q000235:genetics / Q000473:pathology / Q000821:virology; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D005260:Female; D016679:Genome, Viral; D052140:Human papillomavirus 11 / Q000235:genetics* / Q000472:pathogenicity; D052161:Human papillomavirus 18 / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D012516:Osteosarcoma / Q000235:genetics / Q000473:pathology / Q000821:virology; D010212:Papilloma / Q000235:genetics / Q000821:virology*; D030361:Papillomavirus Infections / Q000235:genetics / Q000821:virology*; D011995:Recombination, Genetic; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000473:pathology / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins