title: An ultra-sensitive detection of a whole virus using dual aptamers developed by immobilization-free screening.
abstract: In this study, we successfully developed a ssDNA aptamer pairs by using an advanced immobilization-free SELEX method with affinity-based selection and counter-screening process at every round. By implementing this method, two different aptamers specifically binding to bovine viral diarrhea virus type 1(BVDV type 1) with high affinity were successfully screened. This aptamer pair was applied to ultrasensitive detection platform for BVDV type 1 in a sandwich manner. The ultrasensitive detection of BVDV type 1 using one of aptamers conjugated with gold nanoparticles was obtained in aptamer-aptamer sandwich type sensing format, with the limit of detection of 800 copies/ml, which is comparable to a real-time PCR method.
authors.affiliation: College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-713, South Korea.
authors.forename: Jee-Woong
authors.initials: JW
authors.lastname: Park
authors.forename: Su
authors.initials: S
authors.lastname: Jin Lee
authors.forename: Eun-Jin
authors.initials: EJ
authors.lastname: Choi
authors.forename: Jaejo
authors.initials: J
authors.lastname: Kim
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Song
authors.forename: Man
authors.initials: M
authors.lastname: Bock Gu
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000175:diagnosis* / Q000821:virology; D002417:Cattle / Q000821:virology*; D030241:Diarrhea Virus 1, Bovine Viral / Q000302:isolation & purification*; D057230:Limit of Detection; D052156:SELEX Aptamer Technique / Q000379:methods
chemical_list: D052157:Aptamers, Nucleotide
keywords: Bovine viral diarrhea virus (BVDV); Dual aptamers; SELEX; SPR; Sandwich