title: A new amperometric glucose biosensor based on one-step electrospun poly(vinyl alcohol)/chitosan nanofibers.
abstract: In this work, a new glucose amperometric biosensor was developed by directly electrospinning poly(vinyl alcohol)/chitostan nanofibers on the surface of the platinum electrode, in which glucose oxidase (GOD) was effectively immobilized in nanofibers by encapsulation. After been cross-linked in glutaraldehyde vapor and modified with a thin nafion film, the nanofibers (PVA/chitosan/GOD)/nafion electrode was used for glucose amperometric measurements. The electrospun nanofibrous enzyme membrane served as a better sensing element than the casing one due to the unique properties of nanofibers such as the special three-dimensional network structure, large pores, high porosity, and large surface to volume ratios. The as-prepared biosensor showed a wide linear calibration range, low detection limit, and low apparent Michaelis-Menten constant in the glucose determination. The stability, reproducibility and anti-interference capability of biosensor were also presented. Furthermore, the new biosensor was successfully applied to detect glucose in human serum samples.
authors.affiliation: Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Su
authors.forename: Jianfei
authors.initials: J
authors.lastname: Wei
authors.forename: Xiangling
authors.initials: X
authors.lastname: Ren
authors.forename: Linlin
authors.initials: L
authors.lastname: Li
authors.forename: Xianwei
authors.initials: X
authors.lastname: Meng
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.forename: Fangqiong
authors.initials: F
authors.lastname: Tang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D048271:Chitosan / Q000737:chemistry*; D003217:Conductometry / Q000295:instrumentation*; D004563:Electrochemistry / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation; D011142:Polyvinyl Alcohol / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D011142:Polyvinyl Alcohol; D048271:Chitosan; D005949:Glucose Oxidase; D005947:Glucose