title: Multivariate optimization of separation conditions for simultaneous determination of acemetacin and chlorzoxazone in a pharmaceutical preparation by HPLC using response surface methodology.
abstract: A new, fast, accurate, precise, and sensitive RP-HPLC method for the simultaneous determination of acemetacin and chlorzoxazone has been developed. Response surface methodology with a central composite design was used to optimize the acetonitrile and ammonium acetate percentage in the mobile phase and pH of ammonium acetate. The optimum separation was achieved on a C18 column (250 x 4.6 mm id, 5 microm particle size) using the mobile phase methanol-acetonitrile-0.02 M ammonium acetate, pH 9.4 (25 + 35 + 40, v/v/v) at a flow rate of 1.5 mL/min; UV detection at 270 nm, and cyanocobalamin as an internal standard. This developed method was validated and successfully applied to a coated tablet pharmaceutical preparation.
authors.affiliation: University of Ankara, Faculty of Pharmacy, Department of Analytical Chemistry, 06100 Tandogan, Ankara, Turkey.
authors.forename: Cigdem
authors.initials: C
authors.lastname: Aybaba
authors.forename: Ismail Murat
authors.initials: IM
authors.lastname: Palabiyik
authors.forename: Mehmet Gokhan
authors.initials: MG
authors.lastname: Caglayan
authors.forename: Feyyaz
authors.initials: F
authors.lastname: Onur
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D002753:Chlorzoxazone / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007213:Indomethacin / Q000031:analogs & derivatives* / Q000032:analysis; D009125:Muscle Relaxants, Central / Q000032:analysis*; D013607:Tablets
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D009125:Muscle Relaxants, Central; D013607:Tablets; C026784:acemetacin; D002753:Chlorzoxazone; D007213:Indomethacin