title: [Biosensors as a tool to use in the experiments on blood and selected blood proteins].
abstract: Over last few years, biosensors have become increasingly used as a research tool. Using innovative techniques of detection and estimation of the strength of intermolecular bonds, is particularly important in biochemical processes, including the study of mechanisms of interactions between plasma proteins in the circulatory system. With the numerous enhancements biosensors have become extremely sensitive devices, allowing for analysis of diverse biological material. Moreover, the use of immobilization techniques enables to use sample repeatedly, which significantly reduces costs and the ability to monitor tests in real-time shorten the time of experiment. The presented work discusses examples of the usage of biosensors in the research on the mechanisms of the interactions of blood plasma proteins. The experiments on cancer biomarkers present in the blood circulation system, protein C deficiency causing non-controlled hemorrhagic accidents, and on the level of fibrinogen, as well as 20S proteasome concentration in plasma, are just some examples of biosensors usage in the analyses of blood. They are also applicable in the research work performed the project WROVASC--Integrated Cardiovascular Center, concerning the mechanisms of anticoagulant activity in blood plasma of the polyphenolic-polysaccharide macromolecules of plant origin.
authors.affiliation: Wrovasc--Zintegrowane Centrum Medycyny, Sercowo-Naczyniowej, Wojewódzki Szpital Specjalistyczny we Wrocławiu, Ośrodek Badawczo-Rozwojowy. izabela.pawlaczyk@pwr.wroc.pl
authors.forename: Izabela
authors.initials: I
authors.lastname: Pawlaczyk
authors.forename: Rafał
authors.initials: R
authors.lastname: Ziewiecki
authors.forename: Leszek
authors.initials: L
authors.lastname: Czerchawski
authors.forename: Hubert
authors.initials: H
authors.lastname: Krotkiewski
authors.forename: Roman
authors.initials: R
authors.lastname: Gancarz
mesh_terms: D000925:Anticoagulants / Q000097:blood; D015415:Biomarkers / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood*; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation* / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis*; D003363:Cost Control; D006470:Hemorrhage / Q000097:blood; D006801:Humans
chemical_list: D000925:Anticoagulants; D015415:Biomarkers; D014408:Biomarkers, Tumor; D001798:Blood Proteins