title: Study of total phenol, flavonoids contents and phytochemical screening of various leaves crude extracts of locally grown Thymus vulgaris.
abstract: OBJECTIVE
  To prepare various crude extracts using different polarities of solvent and to quantitatively evaluate their total phenol, flavonoids contents and phytochemical screening of Thymus vulgaris collected from Al Jabal Al Akhdar, Nizwa, Sultanate of Oman.
  
  
  METHODS
  The leave sample was extracted with methanol and evaporated. Then it was defatted with water and extracted with different polarities organic solvents with increasing polarities. The prepare hexane, chloroform, ethyl acetate, butanol and methanol crude extracts were used for their evaluation of total phenol, flavonoids contents and phytochemical screening study. The established conventional methods were used for quantitative determination of total phenol, flavonoids contents and phytochemical screening.
  
  
  RESULTS
  Phytochemical screening for various crude extracts were tested and shown positive result for flavonoids, saponins and steroids compounds. The result for total phenol content was the highest in butanol and the lowest in methanol crude extract whereas the total flavonoids contents was the highest in methanol and the lowest hexane crude extract.
  
  
  CONCLUSIONS
  The crude extracts from locally grown Thymus vulgaris showed high concentration of flavonoids and it could be used as antibiotics for different curable and uncurable diseases.
authors.affiliation: School of Pharmacy, College of Pharmacy and Nursing, University of Nizwa, P. O. Box 33, Postal Code 616, Nizwa, Sultanate of Oman. hossainabi@gmail.com
authors.forename: Mohammad Amzad
authors.initials: MA
authors.lastname: Hossain
authors.forename: Khulood Ahmed Salim
authors.initials: KA
authors.lastname: AL-Raqmi
authors.forename: Zawan Hamood
authors.initials: ZH
authors.lastname: AL-Mijizy
authors.forename: Afaf Mohammed
authors.initials: AM
authors.lastname: Weli
authors.forename: Qasim
authors.initials: Q
authors.lastname: Al-Riyami
mesh_terms: D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D005707:Gallic Acid; D000432:Methanol / Q000737:chemistry; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D011794:Quercetin / Q000032:analysis / Q000737:chemistry; D046930:Thymus Plant / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D005707:Gallic Acid; D011794:Quercetin; D000432:Methanol
keywords: Flavonoids contents; Phytochemical screening; Soxhlet extractor; Thymus vulgaris L.; Total phenol; Various crude extracts