title: Transcriptome profile reveals heat response mechanism at molecular and metabolic levels in rice flag leaf.
abstract: Flag leaf is one of the key photosynthesis organs during rice reproductive stage. A time course microarray analysis of rice flag leaf was done after 40°C treatment for 0 min, 20 min, 60 min, 2h, 4h, and 8h. The identified significant heat responsive genes were mainly involved in transcriptional regulation, transport, protein binding, antioxidant, and stress response. KMC analysis discovered the time-dependent gene expression pattern under heat. MapMan analysis demonstrated that, under heat treatment, Hsp genes and genes involved in glycolysis and ubiquitin-proteasome were enhanced, and genes involved in TCA, carotenoid, dihydroflavonol and anthocyanin metabolisms and light-reaction in the photosynthesis were widely repressed. Meanwhile, some rate-limiting enzyme genes in shikimate, lignin, and mevalonic acid metabolisms were up-regulated, revealing the importance of maintaining specific secondary metabolites under heat stress. The present study increased our understanding of heat response in rice flag leaf and provided good candidate genes for crop improvement.
authors.affiliation: Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China.
authors.forename: Xianwen
authors.initials: X
authors.lastname: Zhang
authors.forename: Wirat
authors.initials: W
authors.lastname: Rerksiri
authors.forename: Ailing
authors.initials: A
authors.lastname: Liu
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Zhou
authors.forename: Hairong
authors.initials: H
authors.lastname: Xiong
authors.forename: Jianhua
authors.initials: J
authors.lastname: Xiang
authors.forename: Xinbo
authors.initials: X
authors.lastname: Chen
authors.forename: Xingyao
authors.initials: X
authors.lastname: Xiong
mesh_terms: D000222:Adaptation, Physiological; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018869:Heat-Shock Response / Q000235:genetics; D006358:Hot Temperature; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome
chemical_list: D010940:Plant Proteins
keywords: GO; HR; Heat stress; Hsf; Hsp; K-means clustering; KMC; Leaf; MVA; Metabolism; Microarray; Rice; TCA; TF; UPS; gene ontology; heat shock protein; heat shock transcription factor; heat-responsive; mevalonic acid; qRT-PCR; quantitative real-time PCR; transcription factor; tricarboxylic acid cycle; ubiquitin-proteasome system