title: Novel albendazole formulations given during the intestinal phase of Trichinella spiralis infection reduce effectively parasitic muscle burden in mice.
abstract: Trichinellosis is a zoonotic disease affecting people all over the world, for which there is no speedy and reliable treatment. Albendazole (ABZ), an inexpensive benzimidazole used in oral chemotherapy against helminthic diseases, has a broad spectrum activity and is well tolerated. However, the low absorption and variable bioavailability of the drug due to its low aqueous solubility are serious disadvantages for a successful therapy. In this study, we evaluated the in vivo antiparasitic activity of three novel solid microencapsulated formulations, designed to improve ABZ dissolution rate, in a murine model of trichinellosis. Both ABZ and the microparticulate formulations were administered during the intestinal phase of the parasite cycle, on days 5 and 6 post-infection. This protocol significantly decreased muscle larval burden measured in the parenteral phase, on day 30 post-infection, when compared with the untreated control. Moreover, two of the three microencapsulated formulations both strongly and consistently reduced worm burden.
authors.affiliation: Departamento de Farmacia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 570, 2000 Rosario, Argentina; IQUIR-CONICET, Suipacha 570, 2000 Rosario, Argentina.
authors.forename: Agustina
authors.initials: A
authors.lastname: García
authors.forename: María G
authors.initials: MG
authors.lastname: Barrera
authors.forename: Gisela
authors.initials: G
authors.lastname: Piccirilli
authors.forename: María D
authors.initials: MD
authors.lastname: Vasconi
authors.forename: Ricardo J
authors.initials: RJ
authors.lastname: Di Masso
authors.forename: Darío
authors.initials: D
authors.lastname: Leonardi
authors.forename: Lucila I
authors.initials: LI
authors.lastname: Hinrichsen
authors.forename: María C
authors.initials: MC
authors.lastname: Lamas
mesh_terms: D015766:Albendazole / Q000008:administration & dosage* / Q000737:chemistry; D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage* / Q000737:chemistry; D002626:Chemistry, Pharmaceutical; D004195:Disease Models, Animal; D007422:Intestines / Q000469:parasitology; D007814:Larva; D008297:Male; D051379:Mice; D009132:Muscles / Q000469:parasitology; D012996:Solutions; D017160:Trichinella spiralis / Q000187:drug effects*; D014235:Trichinellosis / Q000188:drug therapy* / Q000469:parasitology
chemical_list: D000871:Anthelmintics; D012996:Solutions; D015766:Albendazole
keywords: Albendazole; In vivo antiparasitic assay; Microparticulate formulations; Trichinellosis