title: Protective effects of different extracts of Eucommia ulmoides Oliv. against thioacetamide-induced hepatotoxicity in mice.
abstract: A comparison of analysis in evaluating the hepatoprotective action of fractional ethanolic (F0), ethyl acetic (F1), n-butanol (F2) and aqueous (F3) extracts of E. ulmoides Oliv. (EUO) against thioacetamide (TAA) induced hepatic damage was studied in mice. The extract (453 mg/kg-F0, 104 mg/kg-F1, 95 mg/kg-F2 and 237 mg/kg-F3 body weight, po, once daily for 15 days) restored serum marker enzymes levels to normal in TAA treated mice. The biochemical biomarkers viz., total protein, albumin and total bilirubin were also restored forward normal level expression pattern of liver protein profile of mice by using sodium dodecyl sulfate polyacrylamide gel electrophoresis and two-dimensional gel electrophoresis showed 144 spots in TAA administered group which were significantly reduced in EUO extracts treated group. Among the four extracts ethyl acetate (F1) and n-butanol (F2) extracts showed more significant liver protection. TAA induced injury can be correlated with its high phenolic content in these extracts which may have hepatoprotective effects in regulating liver proteins by scavenging free radicals.
authors.affiliation: Department of Pharmaceutical Engineering, School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Yan
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Zheng
authors.forename: Suqing
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000209:etiology / Q000517:prevention & control*; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D015180:Electrophoresis, Gel, Two-Dimensional; D031283:Eucommiaceae / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008106:Liver Cirrhosis, Experimental / Q000097:blood / Q000139:chemically induced / Q000517:prevention & control*; D051379:Mice; D010636:Phenols / Q000302:isolation & purification / Q000627:therapeutic use; D008517:Phytotherapy; D012997:Solvents; D013481:Superoxides / Q000378:metabolism; D013853:Thioacetamide / Q000633:toxicity
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D010636:Phenols; D012997:Solvents; D013853:Thioacetamide; D013481:Superoxides