title: [Monitoring of enterovirus circulation in Irkutsk region].
abstract: OBJECTIVE
  Monitoring of circulation of enteroviruses (EVI) in Irkutsk Region and study of regional specter of circulating enteroviruses.
  
  
  METHODS
  1419 samples from patients with suspected EVI, contact in foci ofenterovirus infection, acute intestine infections and 964 samples of sewage water were studied in total. In 2011 isolation of viral agents from 97 samples positive on enterovirus by RT-PCR from patients with preliminary EVI diagnosis and 5 samples of sewage water of Irkutsk city was carried out. Transplantable line of human rhabdomyosarcoma RD cell culture was used for isolation of enteroviruses. Infection of cells and 2 serial passages of the studied material were carried out. The isolates were typed in neutralization reaction (NR) with a set of 32 diagnostic type-specific immune sera against viral poliomyelitis I-III; Coxsackie B1-6; Coxsackie A2, A4, A7, A9, A10; ECHO 68 - 71; ECHO 2, 4, 7, 8, 9, 12, 16, 20, 25, 26, 27, 29, 31, 33.
  
  
  RESULTS
  In 2011 circulation of enterovirus serotypes that were previously absent on the territory of the region was established: ECHO 68, ECHO 70, ECHO 71. These strains were isolated from patients, circulation of ECHO 70 serotype was established also in samples of sewage water. The analysis of enterovirus landscape carried out showed the possibility of complication of epidemic situation on the territory of the region due to change of serovariants of causative agents of non-polioenterovirus infections and detection ofepidemically significant enteroviruses - ECHO 68, 70 and 71 serotypes.
  
  
  CONCLUSIONS
  Determination of specter ofenterovirus serotypes, detection of serotypes that had not previously circulated in Irkutsk Region allows to prognose epidemic situation on morbidity of enterovirus infections and timely develop and make decisions for ensuring epidemiologic welfare of the population.
authors.forename: A V
authors.initials: AV
authors.lastname: Sevostianova
authors.forename: T A
authors.initials: TA
authors.lastname: Gavrilova
authors.forename: T I
authors.initials: TI
authors.lastname: Borisova
authors.forename: E I
authors.initials: EI
authors.lastname: Andaev
authors.forename: L P
authors.initials: LP
authors.lastname: Nursaianova
authors.forename: M D
authors.initials: MD
authors.lastname: Bibaeva
authors.forename: M I
authors.initials: MI
authors.lastname: Khakimova
authors.forename: V B
authors.initials: VB
authors.lastname: Kazanova
authors.forename: M M
authors.initials: MM
authors.lastname: Verkhozina
authors.forename: T A
authors.initials: TA
authors.lastname: Kirillova
mesh_terms: D000293:Adolescent; D045744:Cell Line, Tumor / Q000821:virology; D002648:Child; D002675:Child, Preschool; D004770:Enterovirus / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000097:blood / Q000453:epidemiology* / Q000276:immunology / Q000821:virology; D062665:Epidemiological Monitoring; D006801:Humans; D007106:Immune Sera / Q000737:chemistry; D007223:Infant; D058889:Molecular Typing; D009500:Neutralization Tests; D012621:Seasons; D012722:Sewage / Q000821:virology; D012800:Siberia / Q000453:epidemiology
chemical_list: D007106:Immune Sera; D012722:Sewage