title: Increased protein expression of LHCG receptor and 17α-hydroxylase/17-20-lyase in human polycystic ovaries.
abstract: OBJECTIVE
  Does the expression of LHCG receptor (LHCGR) protein and key enzymes in the androgen biosynthetic pathway differ in normal human versus polycystic ovarian tissue?
  
  
  CONCLUSIONS
  LHCGR and 17α-hydroxylase/17-20-lyase (CYP17A1) protein levels are increased in polycystic ovaries (PCOs).
  
  
  BACKGROUND
  The predominant source of excess androgen secretion in women with polycystic ovary syndrome (PCOS) is ovarian theca cells but few studies have directly assessed the presence and abundance of protein for key molecules involved in androgen production by theca, including LHCGR and the rate-limiting enzyme in androgen production, CYP17A1.
  
  
  METHODS
  This is a laboratory-based, cross-sectional study comparing protein expression of key molecules in the androgen biosynthetic pathway in archived ovarian tissue from women with normal ovaries (n = 10) with those with PCOs (n = 16).
  
  
  METHODS
  A quantitative morphometric study was performed using sections of archived human ovaries (n = 26) previously characterized as normal or polycystic. The distribution and abundance of LHCGR, CYP17A1, 3β-hydroxysteroid dehydrogenase type 2 (3βHSDII) and 17β-hydroxysteroid dehydrogenase type 5 (17βHSD5) proteins were evaluated by immunohistochemistry and quantified.
  
  
  RESULTS
  A higher proportion of theca cells from anovulatory PCO expressed LHCGR protein when compared with control ovaries (P = 0.01). A significant increase in the intensity of immunostaining for CYP17A1 was identified in antral follicles in sections of PCO compared with ovaries from normal women (P = 0.04).
  
  
  CONCLUSIONS
  As the study used formalin-fixed ovarian tissue sections, it was not possible to carry out studies 'in vitro' using the same ovarian tissues in order to also demonstrate increased functional activity of LHCGR and CYP17A1.
  
  
  CONCLUSIONS
  The data are in keeping with the results of previous studies in isolated theca cells and support the notion of an intrinsic abnormality of theca cell androgen production in women with PCOS.
  
  
  BACKGROUND
  The research was supported by a Programme Grant, G0802782, from the Medical Research Council (MRC) UK and by the National Institute for Health Research (NIHR) Biomedical Research Centre based at Imperial College Healthcare NHS Trust and Imperial College London. F.V.C was supported by Capes Foundation (Brazilian Ministry of Education). The authors have no conflicts of interest to disclose.
authors.affiliation: Institute of Reproductive and Developmental Biology, Imperial College London, London, UK.
authors.forename: F V
authors.initials: FV
authors.lastname: Comim
authors.forename: K
authors.initials: K
authors.lastname: Teerds
authors.forename: K
authors.initials: K
authors.lastname: Hardy
authors.forename: S
authors.initials: S
authors.lastname: Franks
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D005260:Female; D006801:Humans; D010053:Ovary / Q000378:metabolism* / Q000473:pathology; D011085:Polycystic Ovary Syndrome / Q000378:metabolism* / Q000473:pathology; D011974:Receptors, LH / Q000378:metabolism*; D013254:Steroid 17-alpha-Hydroxylase / Q000378:metabolism*
chemical_list: D011974:Receptors, LH; D015096:3-Hydroxysteroid Dehydrogenases; C501892:CYP17A1 protein, human; D013254:Steroid 17-alpha-Hydroxylase
keywords: 17α-hydroxylase/17-20-lyase; LHCG receptor; ovarian steroidogenesis; polycystic ovary syndrome; theca cells