title: Improvement of the enzymatic hydrolysis of furfural residues by pretreatment with combined green liquor and hydrogen peroxide.
abstract: A potential commercial pretreatment for furfural residues (FRs) was investigated by using a combination of green liquor and hydrogen peroxide (GL-H2O2). The results showed that 56.2% of lignin removal was achieved when the sample was treated with 0.6 g H2O2/g-DS (dry substrate) and 6 mL GL/g-DS at 80 °C for 3 h. After 96 h hydrolysis with 18 FPU/g-cellulose for cellulase, 27 CBU/g-cellulose for β-glucosidase, the glucose yield increased from 71.2% to 83.6%. Ethylenediaminetetraacetic acid was used to reduce the degradation of H2O2, the glucose yield increased to 90.4% after the addition of 1% (w/w). The untreated FRs could bind more easily to cellulase than pretreated FRs could. The structural changes on the surface of sample were characterized by X-ray photoelectron spectroscopy. The results indicated that the surface lignin could be effectively removed during pretreatment, thereby decreasing the enzyme-lignin binding activity. Moreover, the carbonyl from lignin plays an important role in cellulase binding.
authors.affiliation: Department of Chemistry and Chemical Engineering, Beijing Forestry University, Beijing 100083, China.
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Yu
authors.affiliation: Department of Chemistry and Chemical Engineering, Beijing Forestry University, Beijing 100083, China; GuangXi Key Laboratory of Chemistry and Engineering of Forest Products, Nanning 530006, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Chemistry and Chemical Engineering, Beijing Forestry University, Beijing 100083, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xing
authors.affiliation: Department of Chemistry and Chemical Engineering, Beijing Forestry University, Beijing 100083, China.
authors.forename: Li-Wei
authors.initials: LW
authors.lastname: Zhu
authors.affiliation: Department of Chemistry and Chemical Engineering, Beijing Forestry University, Beijing 100083, China; GuangXi Key Laboratory of Chemistry and Engineering of Forest Products, Nanning 530006, China. Electronic address: jiangjx2004@hotmail.com.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Jiang
mesh_terms: D000327:Adsorption; D002480:Cellulase / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry; D005662:Furaldehyde / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006868:Hydrolysis; D056951:Photoelectron Spectroscopy
chemical_list: D004492:Edetic Acid; D006861:Hydrogen Peroxide; D005662:Furaldehyde; D002480:Cellulase
keywords: Enzymatic hydrolysis; Furfural residues; Green liquor; Hydrogen peroxide