title: Explanatory chapter: troubleshooting PCR.
abstract: The purpose of this chapter is to assist with any problems you are having with the polymerase chain reaction (PCR) protocol from General PCR.
authors.affiliation: Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: kirstieadams26@yahoo.com.
authors.forename: Kirstie
authors.initials: K
authors.lastname: Canene-Adams
mesh_terms: D017931:DNA Primers; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase / Q000737:chemistry*
chemical_list: D017931:DNA Primers; D019914:Taq Polymerase
keywords: Annealing; Band amplification; DNA template; Denaturation; Extension; Taq DNA polymerase and primers; Troubleshooting PCR