title: Characterization of a novel nm23 gene and its potential roles in gametogenesis in the prawn Macrobrachium rosenbergii (de Man, 1879) (Crustacea: Decapoda).
abstract: Nm23 is a family of genes encoding the nucleoside diphosphate (NDP) kinase, which functions in a wide variety of biological processes, including growth, development, differentiation and tumor metastasis. In this study, a novel nm23 gene, designated as Mrnm23, was identified from the freshwater giant prawn Macrobrachium rosenbergii. The full-length cDNA was 776bp in length, encoding for a protein of 176 amino acids with one typical NDP kinase domain that harbored all the crucial residues for nucleotide binding and enzymatic activity. Like human novel nm23-H1B, the putative protein contained a unique 21-amino-acid NH2-terminal extension as compared to human nm23 (nm23-H1) homologs. Further, 3 extra amino acid residues prolonged the COOH-terminus. The Mrnm23 was ubiquitously expressed in all tissues examined, including androgenic gland, gill, heart, liver, muscle, ovary, and testis. In situ hybridization to gonad sections indicated that the Mrnm23 mRNA was localized in the cytoplasm of cup-base of differentiating spermatids, in the spike of the umbrella-shaped spermatozoa and in the cytoplasm of the early previtellogenic oocytes, suggesting that the Mrnm23 has potential roles in spermiogenesis and early differentiation of oocyte.
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Certificated by Ministry of Education, College of Fisheries and Life Science, Shanghai Ocean University, 999 Hucheng Huan Road, Shanghai, 201306, PR China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Song
authors.forename: Cui-Yun
authors.initials: CY
authors.lastname: Lu
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Gao-Feng
authors.initials: GF
authors.lastname: Qiu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005260:Female; D005718:Gametogenesis / Q000235:genetics*; D015870:Gene Expression; D008297:Male; D008958:Models, Molecular; D008969:Molecular Sequence Data; D054778:NM23 Nucleoside Diphosphate Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D009928:Organ Specificity / Q000235:genetics; D010053:Ovary / Q000378:metabolism; D033541:Palaemonidae / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D011487:Protein Conformation; D016415:Sequence Alignment; D013737:Testis / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D054778:NM23 Nucleoside Diphosphate Kinases
keywords: DIG; DNA complementary to RNA; EST; ISH; Macrobrachium rosenbergii; NDP; NDP-k; Nm23; Oogenesis; PBS; PCR; RT-PCR; Spermatogenesis; UTR; cDNA; digoxigenin; expressed sequence tags; in situ hybridization; nucleoside diphosphate; nucleoside diphosphate kinase; phosphate-buffered saline; polymerase chain reaction; reverse transcription PCR; untranslated region