title: Directed differentiation and neurite extension of mouse embryonic stem cell on aligned poly(lactide) nanofibers functionalized with YIGSR peptide.
abstract: End-functional PLLA nanofibers were fabricated into mats of random or aligned fibers and functionalized post-spinning using metal-free "click chemistry" with the peptide Tyr-Ile-Gly-Ser-Arg (YIGSR). Fibers that were both aligned and functionalized with YIGSR were found to significantly increase the fraction of mouse embryonic stem cells (mESC) expressing neuron-specific class III beta-tubulin (TUJ1), the level of neurite extension and gene expression for neural markers compared to mESC cultured on random fiber mats and unfunctionalized matrices. Precise functionalization of degradable polymers with bioactive peptides created translationally-relevant materials that capitalize on the advantages of both synthetic and natural systems, while mitigating the classic limitations of each.
authors.affiliation: Department of Polymer Science, The University of Akron, Akron, OH 44325, USA.
authors.forename: Laura A Smith
authors.initials: LA
authors.lastname: Callahan
authors.forename: Sibai
authors.initials: S
authors.lastname: Xie
authors.forename: Ian A
authors.initials: IA
authors.lastname: Barker
authors.forename: Jukuan
authors.initials: J
authors.lastname: Zheng
authors.forename: Darrell H
authors.initials: DH
authors.lastname: Reneker
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Dove
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Becker
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002850:Chromatography, Gel; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D051379:Mice; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D016501:Neurites / Q000187:drug effects / Q000378:metabolism*; D009842:Oligopeptides / Q000494:pharmacology*; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D009842:Oligopeptides; D011091:Polyesters; C081571:tyrosyl-isoleucyl-glycyl-seryl-arginine; C033616:poly(lactide)
keywords: Electrospinning; Nanofiber; Neural differentiation; Polymer; YIGSR