title: Integrated mass spectrometry approach to profile proanthocyanidins occurring in food supplements: analysis of Potentilla erecta L. rhizomes.
abstract: Potentilla erecta (L.) is known for the high concentration of proanthocyanidin oligomers (PAs) in its underground parts. The use of its preparations as food supplements and the lack of chemical-analytical studies concerning with an efficient chromatographic separation procedure for these compounds led us to develop a strategy to profile PAs occurring in this species. Their presence has been rationalised on the basis of mass spectrometric profiles. Direct flow injection/electrospray ionisation (ESI)/ion trap (IT) mass spectrometry was used to investigate the hydroalcoholic extract of P. erecta. To achieve deeper structural information and to focus the analysis on PAs with high polimerisation degree (DP), matrix assisted laser desorption ionisation (MALDI)-time of flight (TOF)-mass spectrometry, was used. Finally, liquid chromatography tandem mass spectrometry (LC-MS(2)) analyses were executed by using a diol stationary phase to detect PAs from DP1 to DP10. Results suggested that all of them were B-type procyanidins with 4→8 linkages.
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Salerno, via Ponte don Melillo, 84084 Fisciano (SA), Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Mari
authors.forename: Daniela
authors.initials: D
authors.lastname: Eletto
authors.forename: Cosimo
authors.initials: C
authors.lastname: Pizza
authors.forename: Paola
authors.initials: P
authors.lastname: Montoro
authors.forename: Sonia
authors.initials: S
authors.lastname: Piacente
mesh_terms: D002851:Chromatography, High Pressure Liquid; D019587:Dietary Supplements / Q000032:analysis*; D015394:Molecular Structure; D031987:Potentilla / Q000737:chemistry*; D044945:Proanthocyanidins / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D044945:Proanthocyanidins; C013221:proanthocyanidin
keywords: LC–ESIMS; MALDIMS; Potentilla erecta; Proanthocyanidins