title: Effects of rutaecarpine on hydrogen peroxide-induced apoptosis in murine hepa-1c1c7 cells.
abstract: The aim of this study was to investigate the inhibitory effects of rutaecarpine on DNA strand breaks and apoptosis induced by hydrogen peroxide (H2O2) in murine Hepa-1c1c7 cells. Oxidative DNA damage was estimated by nuclear condensation assessment, fluorescence-activated cell sorting analysis, and Comet assay. Rutaecarpine inhibited cell death induced by 500 μM H2O2, as assessed by 4',6-diamidino-2-phenylindole (DAPI) staining. Treatment with rutaecarpine reduced the number of DNA strand breaks induced by H2O2, as assessed by DAPI staining and Comet assay, and increased quinone reductase, phosphatidylinositol 3-kinase, and pAkt protein levels, as assessed by western blotting.
authors.affiliation: Department of Animal Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Sung-Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Hyunjin
authors.initials: H
authors.lastname: Ahn
authors.forename: Kung-Woo
authors.initials: KW
authors.lastname: Nam
authors.forename: Kyeong Ho
authors.initials: KH
authors.lastname: Kim
authors.forename: Woongchon
authors.initials: W
authors.lastname: Mar
keywords: Apoptosis; DNA damage; Hydrogen peroxide; Oxidative stress; Quinone reductase; Rutaecarpine