title: Interspecies heterokaryon assay to characterize the nucleocytoplasmic shuttling of herpesviral proteins.
abstract: Nucleocytoplasmic trafficking of proteins plays important roles in processes of the viral life cycle. Interspecies heterokaryon assay is one of the most effective methods to investigate the nucleocytoplasmic trafficking properties of a protein. In our lab, the interspecies heterokaryon assay has been applied to identify a few herpesviral proteins with nucleocytoplasmic shuttling property. In this chapter, the detailed information and methods of the heterokaryon assay are presented.
authors.affiliation: Institute of Biology and Medical Sciences, Soochow University, Jiangsu Suzhou, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.forename: Kezheng
authors.initials: K
authors.lastname: Wang
authors.forename: Chunfu
authors.initials: C
authors.lastname: Zheng
mesh_terms: D000818:Animals; D019556:COS Cells; D036022:Capsid Proteins; D002459:Cell Fusion / Q000379:methods*; D002467:Cell Nucleus / Q000378:metabolism*; D002522:Chlorocebus aethiops; D003593:Cytoplasm / Q000378:metabolism*; D018259:Herpesvirus 1, Human / Q000378:metabolism*; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D021381:Protein Transport; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D036022:Capsid Proteins; C082563:VP19 protein, Human herpesvirus 1; D014764:Viral Proteins