title: Water deficit and aluminum tolerance are associated with a high antioxidative enzyme capacity in Indica rice seedlings.
abstract: Plant growth and productivity are greatly affected due to changes in the environmental conditions. In the present investigation, the interactive effects of two important abiotic stresses, i.e., water deficit and Al toxicity, were examined in the seedlings of two rice (Oryza sativa L.) cvs. Malviya-36 (water deficit/Al sensitive) and Vandana (water deficit/Al tolerant). When 15 days grown seedlings were exposed to water deficit (created with 15 % polyethylene glycol 6000) or Al (1 mM AlCl3) treatment or both the treatments together for 48 h, the lengths of root/shoot, relative water content, and chlorophyll greatly declined in the seedlings of the sensitive cultivar, whereas in the tolerant seedlings, either little or insignificant decline in these parameters was observed due to the treatments. Seedlings subjected to water deficit or Al treatment alone or in combination showed increased intensity of the isoenzyme activity bands of superoxide dismutase (SOD), guaiacol peroxidase (GPX), and ascorbate peroxidase (APX) in in-gel activity staining studies. Water deficit caused decrease in intensity of catalase (CAT) activity bands; however, when seedlings were exposed to AlCl3 alone or in combination with water deficit, the intensity of the CAT isoforms increased in both the rice cultivars. The level of expression of the activity bands of SOD, CAT, GPX, and APX was always higher in the seedlings of tolerant cv. Vandana compared to the sensitive cv. Malviya-36 under both controls as well as stress treatments. Higher intensity of isozymes representing higher activity levels of antioxidative enzymes in the rice seedlings and their further increase under water deficit, Al exposure, or in combination of both the stresses appears to serve as useful marker for specifying a combination of water deficit and Al tolerance in rice.
authors.affiliation: Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India.
authors.forename: Poonam
authors.initials: P
authors.lastname: Pandey
authors.forename: Rajneesh Kumar
authors.initials: RK
authors.lastname: Srivastava
authors.forename: R S
authors.initials: RS
authors.lastname: Dubey
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D003681:Dehydration / Q000201:enzymology; D012275:Oryza / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D010088:Oxidoreductases / Q000378:metabolism*; D036226:Seedlings / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D014867:Water; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D014874:Water Pollutants, Chemical; D014867:Water; D000535:Aluminum; D010088:Oxidoreductases