title: Measurement of aldosterone in blood.
abstract: This chapter describes the measurement of aldosterone concentration in plasma or serum. Aldosterone specificity is improved by first extracting the aldosterone into dichloromethane. A radioimmunoassay is then performed on the dried and reconstituted extract using an anti-aldosterone antibody and iodinated aldosterone. The antibody-bound fraction is separated from the unbound fraction using activated charcoal. The notes section provides extra information for steps in the assay that can be problematic.
authors.affiliation: Imperial College Healthcare NHS Trust, Charing Cross Hospital, London, UK.
authors.forename: Sophie C
authors.initials: SC
authors.lastname: Barnes
mesh_terms: D000450:Aldosterone / Q000097:blood*; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods
chemical_list: D000450:Aldosterone