title: Immunogenicity and protective efficacy of heparan sulphate binding proteins of Entamoeba histolytica in a guinea pig model of intestinal amoebiasis.
abstract: Entamoeba histolytica infection is associated with considerable morbidity and mortality in the form of intestinal and extraintestinal amoebiasis. No vaccine is yet available for amoebiasis. Heparan Sulphate Binding Proteins (HSBPs) from E. histolytica were evaluated for immunogenicity and protective efficacy in a Guinea pig model. Animals were immunized subcutaneously with 30μg of HSBP by three weekly inoculations. The immunogenicity of HSBP was determined by antibody response (IgG, IgM and IgA), splenocyte proliferation assay and in vitro direct amoebicidal assay with splenic lymphocytes and monocytes from vaccinated and control animals. The efficacy of the vaccine was evaluated by challenge infection to vaccinated and control animals by intra-caecal inoculation of E. histolytica trophozoites and comparing gross and histopathological findings in caeca of these animals. HSBP was found to induce specific anti-amoebic response as seen by specific antibody production and direct amoebicidal activity of splenocytes. The vaccine also showed partial protection against challenge infection in vaccinated animals as shown by mild/absent lesions and histopathological findings.
authors.affiliation: Departments of Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, India.
authors.forename: Upninder
authors.initials: U
authors.lastname: Kaur
authors.forename: Sumeeta
authors.initials: S
authors.lastname: Khurana
authors.forename: Uma Nahar
authors.initials: UN
authors.lastname: Saikia
authors.forename: M L
authors.initials: ML
authors.lastname: Dubey
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis / Q000097:blood; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism; D002432:Cecum / Q000469:parasitology / Q000473:pathology; D004195:Disease Models, Animal; D004404:Dysentery, Amebic / Q000276:immunology* / Q000517:prevention & control*; D004748:Entamoeba histolytica / Q000737:chemistry / Q000276:immunology*; D006168:Guinea Pigs; D006497:Heparan Sulfate / Q000378:metabolism*; D007111:Immunity, Cellular; D007136:Immunoglobulins / Q000096:biosynthesis / Q000097:blood; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D009000:Monocytes / Q000276:immunology; D015800:Protozoan Proteins / Q000276:immunology* / Q000378:metabolism; D016052:Protozoan Vaccines / Q000592:standards; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014611:Vaccination
chemical_list: D000913:Antibodies, Protozoan; D002352:Carrier Proteins; D006497:Heparan Sulfate; D007136:Immunoglobulins; D015800:Protozoan Proteins; D016052:Protozoan Vaccines
keywords: Entamoeba histolytica; Guinea pig model amoebiasis; Heparan Sulphate Binding Proteins