title: Cicer α-galactosidase immobilization onto functionalized graphene nanosheets using response surface method and its applications.
abstract: Cicer α-galactosidase was immobilized onto functionalized graphene with immobilization efficiency of 84% using response surface methodology (Box-Behnken design). The immobilized enzyme had higher thermal stability than the soluble one, attractive for industrial applications. Immobilization of the enzyme lowered the Km to 1/3rd compared to the soluble enzyme. Raffinose family oligosaccharides (RFOs) are mainly responsible for flatulence by taking soybean derived food products. The immobilized enzyme can be used effectively for the hydrolysis of RFOs. After ten successive runs, the immobilized enzyme still retained approximately 60% activity, with soybean RFOs. The easy availability of enzyme source, ease of its immobilization on matrices, non-toxicity, increased stability of immobilized enzyme and effective hydrolysis of RFOs increase the Cicer α-galactosidase application in food processing industries.
authors.affiliation: School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Neelesh
authors.initials: N
authors.lastname: Singh
authors.forename: Garima
authors.initials: G
authors.lastname: Srivastava
authors.forename: Mahe
authors.initials: M
authors.lastname: Talat
authors.forename: Himanshu
authors.initials: H
authors.lastname: Raghubanshi
authors.forename: Onkar Nath
authors.initials: ON
authors.lastname: Srivastava
authors.forename: Arvind M
authors.initials: AM
authors.lastname: Kayastha
mesh_terms: D029863:Cicer / Q000201:enzymology*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D049329:Nanostructures / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry*; D013696:Temperature; D000519:alpha-Galactosidase / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D009844:Oligosaccharides; D010940:Plant Proteins; D006108:Graphite; D000519:alpha-Galactosidase
keywords: Flatulence; Functionalized graphene; Immobilization; Response surface methodology; α-Galactosidase