title: Effects of hepatitis B virus X protein on human T cell cytokines.
abstract: Chronic infection with hepatitis B virus (HBV) plays a significant role in hepatocellular carcinoma development. To investigate the effect of hepatitis B virus X protein (HBx) on inflammatory cytokines of human T cell, a eukaryotic expression vector, HBx-pEGFP-C1, was constructed and transfected into the Jurkat human T-cell line. Jurkat cells were transfected transiently using Lipofectamine 2000 and activated by phytohemagglutinin (PHA). Interleukin-1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), IL-4, IL-10, IL-13, and IL-14 mRNA was measured. The results showed that the vector HBx-pEGFP-C1 was successfully constructed, and HBx was expressed in Jurkat cells. Compared with a control group, mRNA of IL-1β and TNF-α was significantly elevated in the HBx-pEGFP-C1 group (p < 0.05), while IL-4, IL-10, IL-13, and IL-14 mRNA was decreased (p < 0.05). Therefore, transient overexpression of HBx promoted PHA-induced pro-inflammatory cytokine secretion and repressed anti-inflammatory cytokine secretion in human T cells.
authors.affiliation: Department of Central Laboratory, Songjiang Hospital Affiliated First People's Hospital, Shanghai Jiao Tong University, Shanghai 201600, People's Republic of China.
authors.forename: XiaoLi
authors.initials: X
authors.lastname: Lou
authors.forename: YanQiang
authors.initials: Y
authors.lastname: Hou
authors.forename: DongYu
authors.initials: D
authors.lastname: Liang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000821:virology; D016207:Cytokines / Q000276:immunology*; D005786:Gene Expression Regulation; D006515:Hepatitis B virus / Q000502:physiology; D019694:Hepatitis B, Chronic / Q000276:immunology* / Q000821:virology*; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics; D019169:Jurkat Cells; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism*; D014158:Transcription, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D016207:Cytokines; D053583:Interleukin-1beta; D015534:Trans-Activators; D014409:Tumor Necrosis Factor-alpha; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein