title: Structure-based gene targeting discovery of sphaerimicin, a bacterial translocase I inhibitor.
abstract: Rise and shine: Using a gene-targeting approach aimed at identifying potential L-threonine:uridine-5'-transaldolases that catalyze the formation of (5'S,6'S)-C-glycyluridine, a new bacterial translocase I inhibitor was discovered from an actinomycete following fermentation optimization.
authors.affiliation: Natural Product Research Group, Discovery Science and Technology Department, Drug Discovery and Biomedical Technology Unit, Daiichi Sankyo RD Novare Co., Ltd. Tokyo 134-8630 (Japan).
authors.forename: Masanori
authors.initials: M
authors.lastname: Funabashi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Baba
authors.forename: Toshio
authors.initials: T
authors.lastname: Takatsu
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kizuka
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ohata
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tanaka
authors.forename: Koichi
authors.initials: K
authors.lastname: Nonaka
authors.forename: Anatol P
authors.initials: AP
authors.lastname: Spork
authors.forename: Christian
authors.initials: C
authors.lastname: Ducho
authors.forename: Wei-Chen Leyla
authors.initials: WC
authors.lastname: Chen
authors.forename: Steven G
authors.initials: SG
authors.lastname: Van Lanen
mesh_terms: D039903:Actinobacteria / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D018390:Gene Targeting / Q000379:methods*; D014153:Transaldolase / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D014153:Transaldolase
keywords: actinomycetes; bacterial translocase I inhibitor; gene targeting; nucleoside antibiotics; transaldolase