title: Antishigellosis and Cytotoxic Potency of Crude Extracts and Isolated Constituents from Duranta repens.
abstract: The crude ethanol extracts (stem and fruits), their fractions and two triterpenes, β-Amyrin and 12-Oleanene 3β, 21β-diol, isolated as a mixture from the chloroform soluble fraction of an ethanolic extract of Duranta repens stem, were evaluated for antibacterial, antifungal activities by the disc diffusion method and cytotoxicity by brine shrimp lethality bioassay. The structures of the two compounds were confirmed by IR, (1)H-NMR, (13)C-NMR and LC-MS spectral data. The chloroform soluble fraction of stem and ethanol extract of fruits possess potent antishigellosis activity and also exhibited moderate activity against some pathogenic bacteria and fungi but the isolated compound 1 (mixture of β-Amyrin and 12-Oleanene 3β, 21β-diol) showed mild to moderate inhibitory activity to microbial growth. The minimum inhibitory concentrations (MICs) of the extracts (stem and fruits), their fractions and compound 1 were found to be in the range of 32~128 µg/ml. The chloroform soluble fractions of stem and ethanol extract of fruit showed significant cytotoxicity with LC50 value of 0.94 µg/ml and 0.49 µg/ml, respectively against brine shrimp larvae.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Rajshahi-6205, Bangladesh.
authors.forename: Farjana
authors.initials: F
authors.lastname: Nikkon
authors.forename: M Rowshanul
authors.initials: MR
authors.lastname: Habib
authors.forename: M Rezaul
authors.initials: MR
authors.lastname: Karim
authors.forename: M Shamim
authors.initials: MS
authors.lastname: Hossain
authors.forename: M Ashik
authors.initials: MA
authors.lastname: Mosaddik
authors.forename: M Ekramul
authors.initials: ME
authors.lastname: Haque
keywords: 12-Oleanene-3-β; 21-β-diol; Antimicrobial activity; Cytotoxicity; Duranta repens; β-Amyrin