title: Photoprotective effects of Romanian propolis on skin of mice exposed to UVB irradiation.
abstract: We aimed at investigating the antioxidant, antiinflamatory, antiapoptotic and antigenotoxic effects of a Romanian Propolis (RP) extract in two concentrations (RP1 3 mg, respectively RP2 1.5 mg polyphenols/cm(2)), topically administered, either prior to or after UVB exposure, in a Swiss mouse model. Our results showed that both concentrations of RP extract, independent of the time of administration, significantly attenuated the malondialdehyde (MDA) formation and restored glutathione peroxidase (GPx) activity. However, the 8-hydroxy-2-deoxy-guanosine (8-oxo-dG), nitric oxide (NO) and glutathione (GSH) levels were not influenced by UVB exposure and RP treatment. Interleukin (IL)-6 levels were significantly decreased by RP treatment, both before and after UVB-exposure. RP2 extract, in both regimens, significantly reduced the epidermal hyperplasia and dermal inflammation, whereas RP1 pre-treatment diminished only the dermal inflammation. The effect of our RP extract in terms of reduction of sunburn cell formation and of activated caspase-3 and TUNEL-positive cells was observed in both subsets of the experiment, RP2 having a slightly better protective effect as compared to RP1. The antigenotoxic effect of RP was demonstrated by significantly reduced cyclobutane pyrimidine dimers (CPDs) formation. Our results suggest that RP extract might be a potential chemopreventive candidate by modulation of multiple UVB-induced signaling pathways in skin.
authors.affiliation: Department of Pathology, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania.
authors.forename: Pompei
authors.initials: P
authors.lastname: Bolfa
authors.forename: Raluca
authors.initials: R
authors.lastname: Vidrighinescu
authors.forename: Andrei
authors.initials: A
authors.lastname: Petruta
authors.forename: Dan
authors.initials: D
authors.lastname: Dezmirean
authors.forename: Laura
authors.initials: L
authors.lastname: Stan
authors.forename: Laurian
authors.initials: L
authors.lastname: Vlase
authors.forename: Grigore
authors.initials: G
authors.lastname: Damian
authors.forename: Cornel
authors.initials: C
authors.lastname: Catoi
authors.forename: Adriana
authors.initials: A
authors.lastname: Filip
authors.forename: Simona
authors.initials: S
authors.lastname: Clichici
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000287:Administration, Topical; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D053148:Caspase 3 / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006965:Hyperplasia / Q000188:drug therapy; D015850:Interleukin-6 / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D059808:Polyphenols / Q000032:analysis / Q000494:pharmacology; D011429:Propolis / Q000737:chemistry* / Q000494:pharmacology*; D011740:Pyrimidine Dimers / Q000378:metabolism; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D011855:Radiodermatitis / Q000188:drug therapy / Q000473:pathology; D012383:Romania; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D000975:Antioxidants; D015850:Interleukin-6; D059808:Polyphenols; D011740:Pyrimidine Dimers; D011837:Radiation-Protective Agents; D009569:Nitric Oxide; D000080242:8-Hydroxy-2'-Deoxyguanosine; D011429:Propolis; D053148:Caspase 3; D003849:Deoxyguanosine; D005978:Glutathione
keywords: Apoptosis; Genotoxic lesions; Inflammation; Romanian propolis; Ultraviolet radiation