title: Interaction of cationic antimicrobial peptides with Mycoplasma pulmonis.
abstract: We investigated the mode of action underlying the anti-mycoplasma activity of cationic antimicrobial peptides (AMPs) using four known AMPs and Mycoplasma pulmonis as a model mycoplasma. Scanning electron microscopy revealed that the integrity of the M. pulmonis membrane was significantly damaged within 30 min of AMPs exposure, which was confirmed by measuring the uptake of propidium iodine into the mycoplasma cells. The anti-mycoplasma activity of AMPs was found to depend on the binding affinity for phosphatidylcholine, which was incorporated into the mycoplasma membrane from the growth medium and preferentially distributed in the outer leaflet of the lipid bilayer.
authors.affiliation: Department of Biotechnology, Hoseo University, Asan City, Chungnam 336-795, South Korea.
authors.forename: Ho Jin
authors.initials: HJ
authors.lastname: Park
authors.forename: Ki Mo
authors.initials: KM
authors.lastname: Kang
authors.forename: Kevin
authors.initials: K
authors.lastname: Dybvig
authors.forename: Bok Luel
authors.initials: BL
authors.lastname: Lee
authors.forename: Yong Woo
authors.initials: YW
authors.lastname: Jung
authors.forename: In Hee
authors.initials: IH
authors.lastname: Lee
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D002855:Chromatography, Thin Layer; D007612:Kanamycin / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D045803:Mycoplasma pulmonis / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D010743:Phospholipids / Q000737:chemistry
chemical_list: D023181:Antimicrobial Cationic Peptides; D010743:Phospholipids; D007612:Kanamycin
keywords: 1-palmytoyl-2-oleoyl l-α-phosphatidylcholine; 1-palmytoyl-2-oleoyl l-α-phosphatidylglycerol; AMP; Action mechanism; Antimicrobial peptide; CL; EPS; Liposome; Mycoplasma; NaP; PI; POPC; POPG; Phospholipid; SEM; SPM; TLC; Vsa; antimicrobial peptide; cardiolipin; exopolysaccharide; propidium iodine; scanning electron microscopy; sodium phosphate; sphingomyelin; two-dimensional thin layer chromatography; variable surface antigen