title: Prevalence of adenovirus in children with acute respiratory tract infection in Lanzhou, China.
abstract: BACKGROUND
  Human adenovirus (HAdV) is an important agent causing respiratory tract infection in children. Information on the epidemiological and clinical features of HAdV is limited in children with acute respiratory tract infections (ARTIs) in China, especially those of a novel genotype, Ad55.
  
  
  METHODS
  In total, 1169 nasopharyngeal aspirates were collected from children younger than 14 years with ARTIs between November 2006 and November 2009. The polymerase chain reaction (PCR) was used to screen HAdVs. All PCR-positive products were sequenced.
  
  
  RESULTS
  74 of 1169 (6.33%) specimens were positive for HAdVs. Among positive cases, AdV3 (58/74) was detected most frequently, followed by AdV11 (10/74), AdV2 (2/74), AdV7 (2/69), AdV6 (1/74), and AdV1 (1/74). AdV55 was found in one case. The incidence of HAdV infection peaked in children aged 3-7 years. The most common clinical diagnosis was upper respiratory infection, and the most common syndrome was fever and cough.The comparison of HAdV and RSV group revealed that Children infected with group AdV were significant older than children infected with group RSV, had more fever but less frequently wheezing, and cough, crackles, and cyanosis, The duration of hospitalization between the AdV group and RSV group was not significant, but a greater frequency of LRTIs was observed in RSV group.
  
  
  CONCLUSIONS
  HAdV is an important viral agent in children with ARTIs in Lanzhou City, China. Multiple HAdV serotypes co-circulated with Ad3, which was predominant in this 3-year study. The novel AdV55 genotype was found in one case. No fixed seasonal rhythm could be identified.
authors.affiliation: Key Laboratory for Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China. zhaojund@126.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jin
authors.forename: Rong-fang
authors.initials: RF
authors.lastname: Zhang
authors.forename: Zhi-ping
authors.initials: ZP
authors.lastname: Xie
authors.forename: Kun-long
authors.initials: KL
authors.lastname: Yan
authors.forename: Han-chun
authors.initials: HC
authors.lastname: Gao
authors.forename: Jing-rong
authors.initials: JR
authors.lastname: Song
authors.forename: Xin-Hui
authors.initials: XH
authors.lastname: Yuan
authors.forename: Yun-de
authors.initials: YD
authors.lastname: Hou
authors.forename: Zhao-jun
authors.initials: ZJ
authors.lastname: Duan
mesh_terms: D000257:Adenoviridae Infections / Q000453:epidemiology*; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D009305:Nasopharynx / Q000821:virology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012141:Respiratory Tract Infections / Q000453:epidemiology*; D012703:Serotyping