title: Purification, characterization and immunostimulatory activity of polysaccharide from Cipangopaludina chinensis.
abstract: In this study, we investigated the purification, preliminary characterization and immunostimulatory activity in vivo of polysaccharide from Cipangopaludina chinensis (CCPS). Firstly, crude CCPS was prepared by hot water extraction. And the crude CCPS was sequentially purified by chromatography of DEAE-52 and Sephadex G-100, resulting in two purified fractions of CCPS-1 and CCPS-2. We found the two fractions were homogeneous heteropolysaccharides mainly composed of rhamnose and glucose with the average molecular weight of 226 and 235 kDa, respectively. CCPS-2 was quite different from CCPS-1. It had much higher content of uronic acid and sulfuric radical. For immunostimulatory activity in vivo, crude CCPS could significantly increase the thymus and spleen indices, enhance the macrophage function, and increase the level of serum hemolysin in cyclophosphamide-treated mice, suggesting CCPS had a potent immunostimulatory activity and could be explored as a potential natural immunomodulatory agent.
authors.affiliation: College of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian, Jiangsu 223003, PR China.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Xiong
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Jiao
authors.forename: Xirong
authors.initials: X
authors.lastname: Zhao
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Chen
authors.forename: Qianghua
authors.initials: Q
authors.lastname: Zhang
authors.forename: Changxing
authors.initials: C
authors.lastname: Jiang
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology; D049851:Gastropoda / Q000737:chemistry*; D006460:Hemolysin Proteins / Q000097:blood; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D006460:Hemolysin Proteins; D011134:Polysaccharides; D003520:Cyclophosphamide
keywords: Cipangopaludina chinensis; Immunostimulatory activity; Preliminary characterization; Purification