title: [Expression of molecular markers detected by immunohistochemistry and risk of lymph node metastasis in stage T1 and T2 colorecrectal cancers].
abstract: OBJECTIVE
  To study the molecular risk factors of lymph node metastasis in stage T1 and T2 colorectal cancers by tissue microarray and immunohistochemistry techniques.
  
  
  METHODS
  Two hundred and three patients with stage T1 and T2 colorectal carcinoma who underwent radical surgery from 1999 to 2010 in our department were included in this study. Their clinicopathological data were retrospectively analyzed. Expression of the following 14 molecular markers were selected and assayed by tissue microarray and immunohistochemistry: VEGFR-3, HER2, CD44v6, CXCR4, TIMP-1, EGFR, IGF-1R, IGF-2, IGFBP-1, ECAD, MMP-9, RKIP, CD133, MSI. Chi-squared test and logistic regression were used to evaluate the variables as potential risk factors for lymph node metastasis.
  
  
  RESULTS
  The positive expression rates of biomarkers were as following: VEGFR-3 (44.3%), EGFR (30.5%), HER-2 (28.1%), IGF-1R (63.5%), IGF-2 (44.8%), IGFBP-1 (70.9%), ECAD (45.8%), CD44v6 (51.2%), MMP-9 (44.3%), TIMP-1 (41.4%), RKIP (45.3%), CXCR4 (40.9%), and CD133 (49.8%). The positive rate of MSI expression was 22.2%. Both univariate and multivariate analyses showed that VEGFR-3, HER-2, and TIMP-1 were significant predictors of lymph node metastasis. Univariate analysis showed that CD44v6 and CXCR4 were significant significant predictors of lymph node metastasis.
  
  
  CONCLUSIONS
  VEGFR-3, HER2 and TIMP-1 are independent factors for lymph node metastasis in stage T1 and T2 colorectal cancers.
authors.affiliation: State Key Laboratory of Oncology in South China, Department of Colorectal Surgery, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
authors.forename: Fu-long
authors.initials: FL
authors.lastname: Wang
authors.forename: De-sen
authors.initials: DS
authors.lastname: Wan
authors.forename: Zhen-hai
authors.initials: ZH
authors.lastname: Lu
authors.forename: Yu-jing
authors.initials: YJ
authors.lastname: Fang
authors.forename: Li-ren
authors.initials: LR
authors.lastname: Li
authors.forename: Gong
authors.initials: G
authors.lastname: Chen
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Wu
authors.forename: Pei-rong
authors.initials: PR
authors.lastname: Ding
authors.forename: Ling-heng
authors.initials: LH
authors.lastname: Kong
authors.forename: Jun-zhong
authors.initials: JZ
authors.lastname: Lin
authors.forename: Zhi-zhong
authors.initials: ZZ
authors.lastname: Pan
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D053842:Microsatellite Instability; D008875:Middle Aged; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D019718:Receptors, CXCR4 / Q000378:metabolism; D012004:Rectal Neoplasms / Q000378:metabolism / Q000473:pathology; D012189:Retrospective Studies; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism*; D040321:Vascular Endothelial Growth Factor Receptor-3 / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D018960:Hyaluronan Receptors; D018719:Erb-b2 Receptor Tyrosine Kinases; D019718:Receptors, CXCR4; D019715:Tissue Inhibitor of Metalloproteinase-1; D040321:Vascular Endothelial Growth Factor Receptor-3; C112697:CD44v6 antigen; C527276:CXCR4 protein, human; C560822:TIMP1 protein, human; C508053:ERBB2 protein, human; C581309:FLT4 protein, human