title: Analyzing membrane dynamics with live cell fluorescence microscopy with a focus on yeast mitochondria.
abstract: With the availability of increasing numbers of fluorescent protein variants and state-of-the-art imaging techniques, live cell microscopy has become a standard procedure in modern cell biology. Fluorescent markers are used to visualize the dynamic processes that take place in living cells, including the behavior of membrane-bound organelles. Here, we provide two examples of how we analyze the membrane dynamics of mitochondria in living yeast cells using wide field and confocal microscopy: (1) Long-term observation of mitochondrial shape changes using mitochondria-targeted fluorescent proteins and (2) monitoring the behavior of individual mitochondria using a mitochondria-targeted version of a photoconvertible fluorescent protein.
authors.affiliation: Institut für Zellbiologie, Universität Bayreuth, Bayreuth, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Scholz
authors.forename: Johannes
authors.initials: J
authors.lastname: Förtsch
authors.forename: Stefan
authors.initials: S
authors.lastname: Böckler
authors.forename: Till
authors.initials: T
authors.lastname: Klecker
authors.forename: Benedikt
authors.initials: B
authors.lastname: Westermann
mesh_terms: D015966:Gene Expression Regulation, Fungal; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods; D051336:Mitochondrial Membranes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*
chemical_list: D008164:Luminescent Proteins