title: Assaying chromatin sirtuins.
abstract: Most of the sirtuins' nuclear substrates identified so far are histones or other chromatin-associated proteins and, thus, it is of special relevance the development of good biochemical techniques to analyze the biology of these proteins in the context of chromatin. Here, we describe several of the chromatin-based techniques to identify sirtuins' substrates, including a chromatin immunoprecipitation (ChIP) protocol, an acid-extraction protocol, and a nucleosomal immunoprecipitation protocol to analyze putative sirtuin chromatin interactors.
authors.affiliation: The Massachusetts General Hospital Cancer Center, Harvard Medical School, Boston MA 02114 USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhong
authors.affiliation: The Massachusetts General Hospital Cancer Center, Harvard Medical School, Boston MA 02114 USA.
authors.forename: Barbara
authors.initials: B
authors.lastname: Martinez-Pastor
authors.affiliation: The Massachusetts General Hospital Cancer Center, Harvard Medical School, Boston MA 02114 USA.
authors.forename: Dafne M
authors.initials: DM
authors.lastname: Silberman
authors.affiliation: The Massachusetts General Hospital Cancer Center, Harvard Medical School, Boston MA 02114 USA.
authors.forename: Carlos
authors.initials: C
authors.lastname: Sebastian
authors.affiliation: The Massachusetts General Hospital Cancer Center, Harvard Medical School, Boston MA 02114 USA.
authors.forename: Raul
authors.initials: R
authors.lastname: Mostoslavsky
mesh_terms: D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D009707:Nucleosomes / Q000235:genetics / Q000378:metabolism*; D037761:Sirtuins / Q000235:genetics / Q000378:metabolism*
chemical_list: D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D037761:Sirtuins