title: Crystallization of the HigBA2 toxin-antitoxin complex from Vibrio cholerae.
abstract: The genome of Vibrio cholerae encodes two higBA toxin-antitoxin (TA) modules that are activated by amino-acid starvation. Here, the TA complex of the second module, higBA2, as well as the C-terminal domain of the corresponding HigA2 antitoxin, have been purified and crystallized. The HigBA2 complex crystallized in two crystal forms. Crystals of form I belonged to space group P2(1)2(1)2, with unit-cell parameters a = 129.0, b = 119.8, c = 33.4 Å, and diffracted to 3.0 Å resolution. The asymmetric unit is likely to contain a single complex consisting of two toxin monomers and one antitoxin dimer. The second crystal form crystallized in space group P3(2)21, with unit-cell parameters a = 134.5, c = 55.4 Å. These crystals diffracted to 2.2 Å resolution and probably contain a complex with a different stoichiometry. Crystals of the C-terminal domain of HigA2 belonged to space group C2, with unit-cell parameters a = 115.4, b = 61.2, c = 73.8 Å, β = 106.7°, and diffracted to 1.8 Å resolution.
authors.affiliation: Structural Biology Brussels, Department of Biotechnology, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel, Belgium.
authors.forename: San
authors.initials: S
authors.lastname: Hadži
authors.forename: Abel
authors.initials: A
authors.lastname: Garcia-Pino
authors.forename: Sergio
authors.initials: S
authors.lastname: Martinez-Rodriguez
authors.forename: Koen
authors.initials: K
authors.lastname: Verschueren
authors.forename: Mikkel
authors.initials: M
authors.lastname: Christensen-Dalsgaard
authors.forename: Kenn
authors.initials: K
authors.lastname: Gerdes
authors.forename: Jurij
authors.initials: J
authors.lastname: Lah
authors.forename: Remy
authors.initials: R
authors.lastname: Loris
mesh_terms: D000992:Antitoxins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002772:Cholera Toxin / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D013058:Mass Spectrometry; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D014734:Vibrio cholerae / Q000737:chemistry* / Q000235:genetics / Q000472:pathogenicity
chemical_list: D000992:Antitoxins; D011994:Recombinant Proteins; D002772:Cholera Toxin
keywords: intrinsic disorder; macromolecular complexes; persistence; ribonucleases; toxin–antitoxin modules