title: Characterization and hypoglycemic effect of a polysaccharide extracted from the fruit of Lycium barbarum L.
abstract: Diabetes mellitus is a group of complicated metabolic disorders characterized by high blood glucose level and inappropriate insulin secreting capacity due to decreased glucose metabolism and pancreatic β cell mass or dysfunction of β cells. Thus, improving glucose metabolism and preserving β cell mass and function might be useful for the treatment of diabetes. In this study, a novel acidic polysaccharide LBP-s-1 extracted from Lycium barbarum L. was obtained by purification using macroporous resin and ion-exchanged column. Monosaccharide composition analysis indicated that LBP-s-1 was comprised of rhamnose, arabinose, xylose, mannose, glucose, galactose, galacturonic acid in the molar ratio of 1.00:8.34:1.25:1.26:1.91:7.05:15.28. The preliminary structure features of LBP-s-1 were investigated by FT-IR, (1)H NMR and (13)C NMR. In vitro and in vivo hypoglycemic experiments showed that LBP-s-1 had significant hypoglycemic effects and insulin-sensitizing activity through increasing glucose metabolism and insulin secretion and promoting pancreatic β cell proliferation. Preliminary mechanisms were also elucidated.
authors.affiliation: State Key Laboratory of Natural Medicines, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Juping
authors.initials: J
authors.lastname: Yu
authors.forename: Shan
authors.initials: S
authors.lastname: Zou
authors.forename: Jiajia
authors.initials: J
authors.lastname: Wang
authors.forename: Wenbing
authors.initials: W
authors.lastname: Yao
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Gao
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D032305:Lycium / Q000737:chemistry*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D016062:Porosity; D051381:Rats
chemical_list: D004365:Drugs, Chinese Herbal; D007004:Hypoglycemic Agents; D007328:Insulin; D009005:Monosaccharides; C500223:lycium barbarum polysaccharide; D005947:Glucose
keywords: Glucose utilization; Hypoglycemic effect; Insulin resistance; Lycium barbarum polysaccharide