title: Bacterial Lipopolysaccharides Induce Steroid Sulfatase Expression and Cell Migration through IL-6 Pathway in Human Prostate Cancer Cells.
abstract: Steroid sulfatase (STS) is responsiblefor the conversion of estrone sulfate to estrone that can stimulate growth in endocrine-dependent tumors such as prostate cancer. Although STS is considered as a therapeutic target for the estrogen-dependent diseases, cellular function of STS are still not clear. Previously, we found that tumor necrosis factor (TNF)-α significantly enhances steroid sulfatase expression in PC-3 human prostate cancer cells through PI3K/Akt-dependent pathways. Here, we studied whether bacterial lipopolysaccharides (LPS) which are known to induce TNF-α may increase STS expression. Treatment with LPS in PC-3 cells induced STS mRNA and protein in concentration- and time-dependent manners. Using luciferase reporter assay, we found that LPS enhanced STS promoter activity. Moreover, STS expression induced by LPS increased PC-3 tumor cell migration determined by wound healing assay. We investigated that LPS induced IL-6 expression and IL-6 increased STS expression. Taken together, these data strongly suggest that LPS induces STS expression through IL-6 pathway in human prostate cancer cells.
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Im
authors.forename: Na-Hee
authors.initials: NH
authors.lastname: Park
authors.forename: Yeo-Jung
authors.initials: YJ
authors.lastname: Kwon
authors.forename: Sangyun
authors.initials: S
authors.lastname: Shin
authors.forename: Donghak
authors.initials: D
authors.lastname: Kim
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Chun
keywords: Interleukin-6; Lipopolysaccharides; PC-3; Steroid sulfatase; Tumor cell migration