title: BioBrick™ assembly using the In-Fusion PCR Cloning Kit.
abstract: Synthetic biologists assemble genetic circuits from standardized biological parts called BioBricks™. BioBrick™ examples include promoters, ribosome binding sites, DNA or RNA-coding sequences, and transcriptional terminators. Standard BioBrick™ assembly normally involves assembly of two BioBricks™ at a time using restriction enzymes and DNA ligase. Here we describe an alternative BioBrick™ assembly protocol that describes the assembly of two BioBricks™ using the In-Fusion PCR Cloning Kit. This protocol can also be adapted to use similar recombination-based assembly methods, such as SLIC and Gibson assembly.
authors.affiliation: Department of Bioengineering, University of Washington, Seattle, WA, USA.
authors.forename: Sean C
authors.initials: SC
authors.lastname: Sleight
authors.forename: Herbert M
authors.initials: HM
authors.lastname: Sauro
mesh_terms: D019295:Computational Biology / Q000379:methods*; D004274:DNA, Recombinant; D016133:Polymerase Chain Reaction / Q000379:methods*; D058615:Synthetic Biology / Q000379:methods
chemical_list: D004274:DNA, Recombinant