title: Characterization of the nuclear localization signal of the mouse TET3 protein.
abstract: DNA demethylation is associated with gene activation and is mediated by a family of ten-eleven translocation (TET) dioxygenase. The TET3 protein is a 1668-amino-acid DNA demethylase that is predicted to possess five nuclear localization signals (NLSs). In this paper, we used a series of green fluorescent protein-tagged and mutation constructs to identify a conserved NLS (KKRK) embedded between amino acid 1615 and 1618 of mouse TET3. The KKRK sequence facilitates the cytoplasmic protein's translocation into the nucleus. Additionally TET3 may be imported into the nucleus by importin-α and importin-β.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xiao
authors.forename: Xiao-long
authors.initials: XL
authors.lastname: Zhou
authors.forename: Hong-xiao
authors.initials: HX
authors.lastname: Zhang
authors.forename: Kai
authors.initials: K
authors.lastname: Xiong
authors.forename: Yun
authors.initials: Y
authors.lastname: Teng
authors.forename: Xian-ju
authors.initials: XJ
authors.lastname: Huang
authors.forename: Rui
authors.initials: R
authors.lastname: Cao
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Hong-lin
authors.initials: HL
authors.lastname: Liu
mesh_terms: D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D049308:Dioxygenases; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D041681:NIH 3T3 Cells; D019913:Nuclear Localization Signals; D011518:Proto-Oncogene Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D004268:DNA-Binding Proteins; D019913:Nuclear Localization Signals; D011518:Proto-Oncogene Proteins; D049308:Dioxygenases; C551915:Tet3 protein, mouse
keywords: EGFP; GH; GST; NIH 3T3; NLS; PCR; TET; TET1; TET3; enhanced green fluorescent protein; glutathione S-transferase; growth hormone; nuclear localization signal; polymerase chain reaction; ten-eleven translocation dioxygenase; ten-eleven translocation dioxygenase 1; ten-eleven translocation dioxygenase 3