title: Immobilization of sodium alginate sulfates on polysulfone ultrafiltration membranes for selective adsorption of low-density lipoprotein.
abstract: A novel method for the immobilization of sodium alginate sulfates (SAS) on polysulfone (PSu) ultrafiltration membranes to achieve selective adsorption of low-density lipoprotein (LDL) was developed, which involved the photoinduced graft polymerization of acrylamide on the membrane and the Hofmann rearrangement reaction of grafted acrylamide followed by chemical binding of SAS with glutaraldehyde. The surface modification processes were confirmed by attenuated total reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy characterization. Zeta potential and water contact angle measurements were performed to investigate the surface charge and wettability of the membranes. An enzyme-linked immunosorbent assay was used to measure the binding of LDL on plain and modified PSu membranes. It was found that the PSu membrane immobilized with sodium alginate sulfates (PSu-SAS) greatly enhanced the selective adsorption of LDL from protein solutions and the absorbed LDL could be easily eluted with sodium chloride solution, indicating a specific and reversible binding of LDL to SAS, mainly driven by electrostatic forces. Furthermore, the PSu-SAS membrane showed good blood compatibility as examined by platelet adhesion. The results suggest that the PSu-SAS membranes are promising for application in simultaneous hemodialysis and LDL apheresis therapy.
authors.affiliation: College of Materials and Textile Engineering, Jiaxing University, Jiaxing 314001, China. Electronic address: zjxuwangwei@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Huang
authors.forename: Jian-Da
authors.initials: JD
authors.lastname: Cao
authors.forename: Ping
authors.initials: P
authors.lastname: Lan
authors.forename: Wen
authors.initials: W
authors.lastname: Wu
mesh_terms: D020106:Acrylamide / Q000737:chemistry; D000327:Adsorption; D000464:Alginates / Q000737:chemistry*; D000588:Amines / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D008077:Lipoproteins, LDL / Q000302:isolation & purification*; D008567:Membranes, Artificial; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013431:Sulfates / Q000737:chemistry*; D013450:Sulfones / Q000737:chemistry*; D013499:Surface Properties; D014462:Ultrafiltration
chemical_list: D000464:Alginates; D000588:Amines; D006603:Hexuronic Acids; D008077:Lipoproteins, LDL; D008567:Membranes, Artificial; D011108:Polymers; D013431:Sulfates; D013450:Sulfones; D020106:Acrylamide; C017662:polysulfone P 1700; D020723:Glucuronic Acid
keywords: Low-density lipoprotein; Polysulfone; Selective adsorption; Sodium alginate sulfates; Surface modification