title: Lectin-functionalized poly(glycidyl methacrylate)-block-poly(vinyldimethyl azlactone) surface scaffolds for high avidity microbial capture.
abstract: Microbial exopolysaccharides (EPS) play a critical and dynamic role in shaping the interactions between microbial community members and their local environment. The capture of targeted microbes using surface immobilized lectins that recognize specific extracellular oligosaccharide moieties offers a nondestructive method for functional characterization of EPS content. In this report, we evaluate the use of the block copolymer, poly(glycidyl methacrylate)-block-4,4-dimethyl-2-vinylazlactone (PGMA-b-PVDMA), as a surface scaffold for lectin-specific microbial capture. Three-dimensional polymer films were patterned on silicon substrates to provide discrete, covalent coupling sites for Triticum vulgare and Lens culinaris lectins. This material increased the number of Pseudomonas fluorescens microbes captured by up to 43% compared to control scaffolds that did not contain the copolymer. These results demonstrate that PGMA-b-PVDMA scaffolds provide a platform for improved microbe capture and screening of EPS content by combining high avidity lectin surfaces with three-dimensional surface topography.
authors.affiliation: Center for Nanophase Materials Sciences, Oak Ridge National Laboratory , Oak Ridge, Tennessee 37831, United States.
authors.forename: Ryan R
authors.initials: RR
authors.lastname: Hansen
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Hinestrosa
authors.forename: Katherine R
authors.initials: KR
authors.lastname: Shubert
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Morrell-Falvey
authors.forename: Dale A
authors.initials: DA
authors.lastname: Pelletier
authors.forename: Jamie M
authors.initials: JM
authors.lastname: Messman
authors.forename: S Michael
authors.initials: SM
authors.lastname: Kilbey
authors.forename: Bradley S
authors.initials: BS
authors.lastname: Lokitz
authors.forename: Scott T
authors.initials: ST
authors.lastname: Retterer
mesh_terms: D027806:Lens Plant / Q000737:chemistry*; D015394:Molecular Structure; D010316:Particle Size; D037121:Plant Lectins / Q000737:chemistry* / Q000378:metabolism*; D011109:Polymethacrylic Acids / Q000737:chemistry / Q000378:metabolism*; D011145:Polyvinyls / Q000737:chemistry / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000302:isolation & purification*; D013499:Surface Properties; D014908:Triticum / Q000737:chemistry*
chemical_list: D037121:Plant Lectins; D011109:Polymethacrylic Acids; D011145:Polyvinyls