title: Affinity-based fluorogenic labeling of ATP-binding proteins with sequential photoactivatable cross-linkers.
abstract: A specific illumination approach has been developed for identification of adenosine triphosphate (ATP)-binding proteins. This strategy utilizes a tandem photoactivatable unit that consists of a diazirine group as a carbene precursor and an o-hydroxycinnamate moiety as a coumarin precursor. The photolysis of diazirine induces a specific cross-link on target proteins and is followed by photoactivation of coumarin generation with a concomitant release of the pre-installed affinity ligand. The ATP, installed with this cross-linker at the γ-position, successfully transferred a coumarin onto ATP-binding proteins using only UV-irradiation.
authors.affiliation: Laboratory of Biorecognition Chemistry, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan. Electronic address: ttomo@pha.u-toyama.ac.jp.
authors.forename: Takenori
authors.initials: T
authors.lastname: Tomohiro
authors.forename: Hirotsugu
authors.initials: H
authors.lastname: Inoguchi
authors.forename: Souta
authors.initials: S
authors.lastname: Masuda
authors.forename: Yasumaru
authors.initials: Y
authors.lastname: Hatanaka
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000345:Affinity Labels / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry; D003978:Diazomethane / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D005969:Glutamate Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D010782:Photolysis; D013237:Stereoisomerism; D014466:Ultraviolet Rays; D018631:ras Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D000345:Affinity Labels; D003374:Coumarins; D005456:Fluorescent Dyes; D003978:Diazomethane; D000255:Adenosine Triphosphate; C030123:coumarin; D005969:Glutamate Dehydrogenase; D018631:ras Proteins
keywords: ATP; Cinnamate; Coumarin; Diazirine; Photoaffinity labeling