title: Biological activities and physicochemical properties of Maillard reaction products in sugar-bovine casein peptide model systems.
abstract: The purpose of this study was to evaluate the biological activities and physicochemical properties of Maillard reaction products (MRPs), derived from aqueous reducing sugar (ribose, galactose and lactose) and bovine casein peptide (BCP) model systems. The fluorescence intensity of ribose-BCP MRPs reached the maximum value within 1h, while it took 3h for galactose-BCP MRPs. Size exclusion chromatography of all the MRPs indicated molecular rearrangements and production of new smaller molecules, as a function of the heating time. The consumption of ribose and amino groups was the highest in the ribose-BCP MRPs. BCP lost its known angiotensin-I-converting enzyme (ACE) inhibitory activity by the Maillard reaction with reducing sugars. Ribose-BCP MRPs had the lowest ACE inhibitory activity, but they showed the highest 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging activity and ferrous reducing power among all the MRPs. Galactose-BCP MRPs inhibited, slightly the growth of Caco-2 cells, while ribose-BCPand lactose-BCP MRPs had no cytotoxicity.
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, PR China. zhanmei.jiang@gmail.com
authors.forename: Zhanmei
authors.initials: Z
authors.lastname: Jiang
authors.forename: Lizhe
authors.initials: L
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D002364:Caseins / Q000737:chemistry* / Q000494:pharmacology; D002417:Cattle; D002470:Cell Survival; D005690:Galactose / Q000737:chemistry* / Q000494:pharmacology; D005947:Glucose / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D015416:Maillard Reaction; D008956:Models, Chemical; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology; D012266:Ribose / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D002364:Caseins; D010455:Peptides; D012266:Ribose; D005947:Glucose; D005690:Galactose
keywords: ACE inhibitory activity; Antioxidant activity; Antiproliferative activity; Bovine casein peptide; Maillard reaction products