title: Effects of titanium surface topography on morphology and in vitro activity of human gingival fibroblasts.
abstract: OBJECTIVE
  The aim of the present study was to evaluate in vitro the biological behavior of human gingival fibroblasts cultured on two different titanium surfaces.
  
  
  METHODS
  Titanium test disks were prepared with a machined, relatively smooth (S) surface or a rough surface (O) obtained by a double acid etching procedure. Primary cultures of human gingival fibroblasts were plated on the experimental titanium disks and cultured up to 14 days. Titanium disk surfaces were analysed by scanning electron microscopy (SEM). Cell proliferation and a quantitative analysis by ELISA in situ of ECM components as CoI, FN and TN were performed.
  
  
  RESULTS
  Results have shown different effects of titanium surface microtopography on cell expression and differentiation. At 96 hours of culture on experimental surfaces human gingival fibroblasts displayed a favourable cell attachment and proliferation on both surfaces although showing some differences.
  
  
  CONCLUSIONS
  Both the relatively smooth and the etched surfaces interacted actively with in vitro cultures of human gingival fibroblasts, promoting cell proliferation and differentiation. Results suggested that the microtopography of a double acid-etched rough surface may induce a greater Co I and FN production, thus conditioning in vivo the biological behaviour of human gingival fibroblasts during the process of peri-implant soft tissue healing.
authors.affiliation: Department of Oral and Maxillo-Facial Sciences Federico II University, Naples, Italy - luca.ramaglia@unina.it.
authors.forename: L
authors.initials: L
authors.lastname: Ramaglia
authors.forename: G
authors.initials: G
authors.lastname: Capece
authors.forename: G
authors.initials: G
authors.lastname: Di Spigna
authors.forename: M P
authors.initials: MP
authors.lastname: Bruno
authors.forename: N
authors.initials: N
authors.lastname: Buonocore
authors.forename: L
authors.initials: L
authors.lastname: Postiglione
mesh_terms: D001672:Biocompatible Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000295:instrumentation; D002455:Cell Division / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D016895:Culture Media, Serum-Free; D016326:Extracellular Matrix Proteins; D014025:Titanium