title: How pure are your vesicles?
abstract: We propose a straightforward method to estimate the purity of vesicle preparations by comparing the ratio of nano-vesicle counts to protein concentration, using tools such as the increasingly available NanoSight platform and a colorimetric protein assay such as the BCA-assay. Such an approach is simple enough to apply to every vesicle preparation within a given laboratory, assisting researchers as a routine quality control step. Also, the approach may aid in comparing/standardising vesicle purity across diverse studies, and may be of particular importance in evaluating vesicular biomarkers. We herein propose some criteria to aid in the definition of pure vesicles.
authors.affiliation: Institute of Cancer and Genetics, School of Medicine, Velindre Cancer Centre, Cardiff University, Cardiff, UK.
authors.forename: Jason
authors.initials: J
authors.lastname: Webber
authors.forename: Aled
authors.initials: A
authors.lastname: Clayton
keywords: extracellular vesicles; nano-particle tracking; sample purity; vesicle counting