title: Simultaneous analysis of furfural metabolites from Rehmanniae radix preparata by HPLC-DAD-ESI-MS.
abstract: A method based on HPLC with diode array detector (DAD) and electrospray ionisation mass spectrometry (ESI-MS) was established for the simultaneous determination of 5-HMF and its derivatives, including a new 5-HMF derivative, in Rehmanniae radix preparata. Validation parameters, such as linearity, limit of detection, limit of quantification, recovery, accuracy, and precision, were successfully obtained. In addition, the efficiencies of diverse extraction methods were compared for the development of a standard analytical method. The verified method was successfully applied to the quantitative determination of four representative metabolites in eighteen R. radix preparata samples from Korea and China. Additionally, the increase in the amount of 5-HMF derivatives was monitored during the processing of three dried R. radix samples. The results showed that a newly isolated diglycosylated 5-HMF derivative, 5-(α-D-galactopyranosyl-(1→6)-α-D-galactopyranosyloxymethyl)-2-furancarboxaldehyde, appeared in concentrations comparable to that of 5-HMF, suggesting its potential to serve as a marker compound in R. radix preparata.
authors.affiliation: Natural Products Research Institute, College of Pharmacy, Seoul National University, San 56-1, Shillim, Kwanak, Seoul 151-742, Republic of Korea.
authors.forename: Tae Hyung
authors.initials: TH
authors.lastname: Won
authors.forename: Lijuan
authors.initials: L
authors.lastname: Liao
authors.forename: Sam Sik
authors.initials: SS
authors.lastname: Kang
authors.forename: Jongheon
authors.initials: J
authors.lastname: Shin
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000378:metabolism; D005662:Furaldehyde / Q000032:analysis* / Q000378:metabolism; D032267:Rehmannia / Q000737:chemistry* / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D005662:Furaldehyde
keywords: 5-HMF; Furfural compounds; HPLC-DAD-ESI-MS; Rehmannia glutinosa; Rehmanniae radix preparata