title: [Detection of uncultivable bacteria forms in probiotic lyophilized preparations].
abstract: OBJECTIVE
  Detection of viable but uncultivable forms among lyophilized cells of commercial probiotics.
  
  
  METHODS
  9 series of probiotic preparations (colibacterin, bificol, bifidumbacterin, bifiform) with expired (up to 30 years of storage) or valid shelf life were objects of the study. Total quantity of the bacteria was calculated under the microscope in Goryaev chamber, the number of viable cells was determined in luminescence microscope after staining by an array of fluorescent dyes, the quantity of CFU/ml was evaluated by method of seeding into the respective solid or semi-liquid cultivation medium.
  
  
  RESULTS
  Juxtaposition of the specified parameter values allowed to establish that in lyophilized preparations of colibacterin with expired shelf life the amount of viable but not forming colonies (uncultivable forms) cells varied from 4.13 to 99.73% depending on date of production. For bifidumbacterin with unexpired shelf life live cells constituted 95.45 and 70.73%. The amount of viable bifidobacteria forming colonies was on the level of 100 and 50%, respectively. In bifidopreparations micro colonies that may possibly be formed spontaneously by awakened uncultivable forms were noted. A possibility of growth stimulation of these cells by 1 and 10% aminopeptide was shown.
  
  
  CONCLUSIONS
  The presence of uncultivable forms in lyophilized preparations of probiotics was proven experimentally.
authors.forename: L P
authors.initials: LP
authors.lastname: Blinkova
authors.forename: Iu D
authors.initials: IuD
authors.lastname: Pakhomov
authors.forename: O V
authors.initials: OV
authors.lastname: Dmitrieva
mesh_terms: D058491:Bacterial Load; D001430:Bacteriocins / Q000032:analysis*; D001688:Biological Products / Q000032:analysis*; D003087:Colicins / Q000032:analysis*; D015169:Colony Count, Microbial; D003470:Culture Media; D061353:Food Storage; D005612:Freeze Drying; D050296:Microbial Viability / Q000187:drug effects; D010455:Peptides / Q000494:pharmacology; D019936:Probiotics / Q000032:analysis*
chemical_list: D001430:Bacteriocins; D001688:Biological Products; D003087:Colicins; D003470:Culture Media; D010455:Peptides; C010546:bifidobacterin; C012775:bifikol; C016501:colibacterin