title: The effect of PEG-5K grafting level and particle size on tumor accumulation and cellular uptake.
abstract: PEG-modified gold nanoparticles (PEG-modified GNs) with diameters of 40 nm and 70 nm were prepared to elucidate the effect of extent of PEG (M.W. 5000) grafting and particle size on tumor accumulation and cellular uptake. Flow cytometry reveals that cellular uptake is strongly related to the size of PEG-modified GNs, rather than the extent of PEG-5K grafting level. Cytotoxicity analysis based on the intracellular release of drugs showed that the 70 nm PEG-modified GNs have the higher cytotoxicity, beccause of their greater cellular uptake. Also, particle size, rather than PEG-5K grafting level affects tumor accumulation. However, PEG-5K grafting level significantly affects the accumulation of particles in the liver and spleen. This finding is important in determining the proper PEG-5K grafting level and particle size for designing nano-medicines.
authors.affiliation: Department of Biomedical Engineering, National Yang Ming University, Taipei 112, Taiwan, ROC; Biophotonics & Molecular Imaging Research Center (BMIRC), National Yang Ming University, Taipei 112, Taiwan, ROC. Electronic address: cllo@ym.edu.tw.
authors.forename: Chun-Liang
authors.initials: CL
authors.lastname: Lo
authors.forename: Meng-Han
authors.initials: MH
authors.lastname: Chou
authors.forename: Pei-Lin
authors.initials: PL
authors.lastname: Lu
authors.forename: I-Wen
authors.initials: IW
authors.lastname: Lo
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Chiang
authors.forename: Shang-Yu
authors.initials: SY
authors.lastname: Hung
authors.forename: Chieh-Yu
authors.initials: CY
authors.lastname: Yang
authors.forename: Shuian-Yin
authors.initials: SY
authors.lastname: Lin
authors.forename: Shiaw-Pyng
authors.initials: SP
authors.lastname: Wey
authors.forename: Jem-Mau
authors.initials: JM
authors.lastname: Lo
authors.forename: Ging-Ho
authors.initials: GH
authors.lastname: Hsiue
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004705:Endocytosis / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006046:Gold / Q000737:chemistry / Q000493:pharmacokinetics*; D045325:HCT116 Cells; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry / Q000493:pharmacokinetics*
chemical_list: D011092:Polyethylene Glycols; D006046:Gold
keywords: Biodistribution; Cellular uptake; Gold nanoparticles; Polyethylene glycol; Surface modification