title: Expression pattern and subcellular localization of Arabidopsis purple acid phosphatase AtPAP9.
abstract: Purple acid phosphatase (PAP; EC 3.1.3.2) enzymes are metallophosphoesterases that hydrolysis phosphate ester bonds in a wide range of substrates. Twenty-nine PAP-encoding loci have been identified in the Arabidopsis genome, many of which have multiple transcript variants expressed in response to diverse environmental conditions. Having analyzed T-DNA insertion mutants, we have provided strong pieces of evidence that AtPAP9 locus encodes at least two types of transcripts, designated as AtPAP9-1 and AtPAP9-2. These transcript variants expressed distinctly during the course of growth in medium containing sufficient phosphate or none. Further histochemical analysis by the use of AtPAP9-1 promoter fused to β-glucuronidase reporter gene indicated the expression of this gene is regulated in a tissue-specific manner. AtPAP9-1 was highly expressed in stipule and vascular tissue, particularly in response to fungal infection. Subcellular localization of AtPAP9-1:green fluorescent fusion protein showed that it must be involved in plasma membrane and cell wall adhesion.
authors.affiliation: Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14965/161, Tehran, Islamic Republic of Iran. Electronic address: katayounzamani@yahoo.com.
authors.forename: Katayoun
authors.initials: K
authors.lastname: Zamani
authors.affiliation: Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14965/161, Tehran, Islamic Republic of Iran. Electronic address: narcis@nigeb.ac.ir.
authors.forename: Tahmineh
authors.initials: T
authors.lastname: Lohrasebi
authors.affiliation: Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tarbiat Modares University, P. O. Box 14115-336, Tehran, Islamic Republic of Iran. Electronic address: ssabet2000@yahoo.com.
authors.forename: Mohammad S
authors.initials: MS
authors.lastname: Sabet
authors.affiliation: Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14965/161, Tehran, Islamic Republic of Iran. Electronic address: malboobi@nigeb.ac.ir.
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Malboobi
authors.affiliation: Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14965/161, Tehran, Islamic Republic of Iran. Electronic address: m-amir@nigeb.ac.ir.
authors.forename: Amir
authors.initials: A
authors.lastname: Mousavi
mesh_terms: D000135:Acid Phosphatase / Q000235:genetics* / Q000378:metabolism*; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002462:Cell Membrane / Q000502:physiology; D002473:Cell Wall / Q000502:physiology; D004269:DNA, Bacterial / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000235:genetics; D018520:Plant Shoots / Q000235:genetics; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; D006023:Glycoproteins; D012333:RNA, Messenger; C036483:T-DNA; C091137:purple acid phosphatase; D000135:Acid Phosphatase
keywords: Arabidopsis; AtPAP9; Cell adhesion; Fungal infection; Phosphate