title: The effect of ultrasound on enzymatic degumming process of rapeseed oil by the use of phospholipase A(1).
abstract: Comparative studies of enzymatic degumming process of rapeseed oil were carried out in mechanical-stirring and ultrasonic-assisted mechanical-stirring systems. The influences of enzyme dosage (10-50 mg/kg), pH (4.5-6), temperature (45-65 °C), water amount (1-3%), ultrasonic power (0.06-0.09 W/cm(3)) and reaction time were investigated subsequently. A suitable ultrasonic power of 0.07 W/cm(3) was determined to guarantee satisfactory degumming efficiency and enzyme activity. Compared to the mechanical-stirring system, optimum temperature of phospholipase A (PLA) in the ultrasonic-assisted mechanical-stirring system was about 5 °C higher, while the effects of pH on both of the two systems were quite similar. Less time and water were used in the ultrasonic-assisted mechanical-stirring system for enzymatic degumming. The study on the quality changes of degummed oils showed that ultrasound could accelerate the oxidation of edible oils due to the effect of cavitation, thus more attention should be paid on the oxidative stability in the further application.
authors.affiliation: School of Food Science and Technology, Jiangnan University, State Key Laboratory of Food Science and Technology, 1800 Lihu Road, Wuxi 214122, Jiangsu, PR China. Electronic address: jxfhstkad@163.com.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Jiang
authors.forename: Ming
authors.initials: M
authors.lastname: Chang
authors.forename: Xiaosan
authors.initials: X
authors.lastname: Wang
authors.forename: Qingzhe
authors.initials: Q
authors.lastname: Jin
authors.forename: Xingguo
authors.initials: X
authors.lastname: Wang
mesh_terms: D005229:Fatty Acids, Monounsaturated; D005511:Food Handling / Q000379:methods*; D063427:Food Quality; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D054466:Phospholipases A1 / Q000378:metabolism*; D053149:Plant Gums / Q000302:isolation & purification* / Q000378:metabolism; D010938:Plant Oils / Q000737:chemistry*; D000074262:Rapeseed Oil; D013696:Temperature; D014465:Ultrasonics; D014867:Water / Q000737:chemistry
chemical_list: D005229:Fatty Acids, Monounsaturated; D053149:Plant Gums; D010938:Plant Oils; D000074262:Rapeseed Oil; D014867:Water; D054466:Phospholipases A1
keywords: Degumming; Phospholipase A; Rapeseed oil; Ultrasound