title: Novel polymorphisms of the APOA2 gene and its promoter region affect body traits in cattle.
abstract: Apolipoprotein A-II (APOA2) is one of the major constituents of high-density lipoprotein and plays a critical role in lipid metabolism and obesity. However, similar research for the bovine APOA2 gene is lacking. In this study, polymorphisms of the bovine APOA2 gene and its promoter region were detected in 1021 cows from four breeds by sequencing and PCR-RFLP methods. Totally, we detected six novel mutations which included one mutation in the promoter region, two mutations in the exons and three mutations in the introns. There were four polymorphisms within APOA2 gene were analyzed. The allele A, T, T and G frequencies of the four loci were predominant in the four breeds when in separate or combinations analysis which suggested cows with those alleles to be more adapted to the steppe environment. The association analysis indicated three SVs in Nangyang cows, two SVs in Qinchun cows and the 9 haplotypes in Nangyang cows were significantly associated with body traits (P<0.05 or P<0.01). The results of this study suggested the bovine APOA2 gene may be a strong candidate gene for body traits in the cattle breeding program.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Caixia
authors.initials: C
authors.lastname: Li
authors.forename: Hanfang
authors.initials: H
authors.lastname: Cai
authors.forename: Yao
authors.initials: Y
authors.lastname: Xu
authors.forename: Xianyong
authors.initials: X
authors.lastname: Lan
authors.forename: Chuzhao
authors.initials: C
authors.lastname: Lei
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D016633:Apolipoprotein A-II / Q000235:genetics*; D001483:Base Sequence; D001947:Breeding; D002417:Cattle / Q000235:genetics* / Q000254:growth & development; D005260:Female; D050437:Genes, Developmental; D056726:Genetic Association Studies / Q000662:veterinary; D008297:Male; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics*; D019655:Quantitative Trait, Heritable; D013008:Somatotypes / Q000235:genetics*
chemical_list: D016633:Apolipoprotein A-II
keywords: APOA1; APOA2; Apoa2 gene; Apolipoprotein A-II; Association; Body traits; Bovine; CRS; Chinese Red Steppe cattle; HDL; Haplotype; JX; Jiaxian cattle; NY; Nanyang cattle; QC; Qinchuan cattle; SVs; UTR; apolipoprotein A-I; high-density lipoprotein; sequence variants; untranslated regions