title: Species differentiation of Bacteroides dorei from Bacteroides vulgatus and Bacteroides ovatus from Bacteroides xylanisolvens - back to basics.
abstract: We present the results from 16S sequencing and phenotypic tests for differentiation of Bacteroides dorei from Bacteroides vulgatus and Bacteroides ovatus from Bacteroides xylanisolvens, which was not possible with MALDI-TOF MS. Testing with β-glucosidase could differentiate B. dorei from B. vulgatus and a negative catalase reaction could identify B. xylanisolvens.
authors.affiliation: Department of Clinical Microbiology, Odense University Hospital, Odense, Denmark. Electronic address: rune.pedersen@rsyd.dk.
authors.forename: Rune M
authors.initials: RM
authors.lastname: Pedersen
authors.forename: Ea S
authors.initials: ES
authors.lastname: Marmolin
authors.forename: Ulrik S
authors.initials: US
authors.lastname: Justesen
mesh_terms: D015373:Bacterial Typing Techniques; D001439:Bacteroides / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D001617:beta-Glucosidase / Q000032:analysis*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D001617:beta-Glucosidase
keywords: Bacteroides; MALDI-TOF MS; β-Glucosidase