title: Changes in the protein patterns in pea (Pisum sativum L.) roots under the influence of long- and short-term chilling stress and post-stress recovery.
abstract: Amongst many factors restricting geographical distribution of plants and crop productivity, low temperature is one of the most important. To gain better understanding of the molecular response of germinating pea (Pisum sativum L.) to low temperature, we investigated the influence of long and short chilling stress as well as post-stress recovery on the alterations in the root proteomes. The impact of long stress was examined on the pea seeds germinating in the continuous chilling conditions of 10 °C for 8 days (LS). To examine the impact of short stress, pea seeds germinating for 72 h in the optimal temperature of 20 °C were subjected to 24-h chilling (SS). Additionally, both stress treatments were followed by 24 h of recovery in the optimal conditions (accordingly LSR and SR). Using the 2D gel electrophoresis and MALDI-TOF MS protein identification, it was revealed, that most of the proteins undergoing regulation under the applied conditions were implicated in metabolism, protection against stress, cell cycle regulation, cell structure maintenance and hormone synthesis, which altogether may influence root growth and development in the early stages of plant life. The obtained results have shown that most of detected alterations in the proteome patterns of pea roots are dependent on stress duration. However, there are some analogical response pathways which are triggered regardless of stress length. The functions of proteins which accumulation has been changed by chilling stress and post-stress recovery are discussed here in relation to their impact on pea roots development.
authors.affiliation: Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Street 1a, 10-957 Olsztyn, Poland. Electronic address: anna.badowiec@uwm.edu.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Badowiec
authors.forename: Sylwia
authors.initials: S
authors.lastname: Swigonska
authors.forename: Stanisław
authors.initials: S
authors.lastname: Weidner
mesh_terms: D003080:Cold Temperature; D015180:Electrophoresis, Gel, Two-Dimensional; D018525:Germination / Q000502:physiology; D018532:Pisum sativum / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
keywords: 2D; C1, C2; Chilling stress; Germination; LS; LSR; MALDI-TOF MS; PAGE two-dimensional electrophoresis in polyacrylamide gels; Pea roots; Proteomics; ROS; Recovery; SR; SS; control samples of the seeds germinating in the optimal temperature; long, continuous chilling stress; matrix-assisted lased desorption/ionization time-of-flight mass spectrometer; reactive oxygen species; recovery after long chilling stress; recovery after short stress; short, rapid chilling stress