title: Lack of association of a common polymorphism in the 3' -UTR of interleukin 8 with non small cell lung cancer in Kashmir.
abstract: BACKGROUND
  Chronic inflammation is considered as an important factor in the pathogenesis of lung cancer. The presence of inflammatory cells and higher levels of pro-inflammatory cytokines in the tumor microenvironment and their surrounding tissues is gaining much importance in research.
  
  
  METHODS
  One hundred ninety NSCLC cases and 200 age, smoking and sex matched controls were evaluated for association of IL-8 -251 (rs4073) and IL-8 -845 (rs2227532) in our population. Restriction fragment length polymorphism (RFLP) was used followed by direct sequencing for the detection of SNPs.
  
  
  RESULTS
  The IL-8 -845 polymorphism was not found in our population. No significant association was observed between the IL-8 -251 AT genotypes and IL-8 -25 AA genotypes and NSCLC (p=0.05) in our population. The IL-8 -251 A allele was also non-significant (p=0.05) in NSCLC patients.
  
  
  CONCLUSIONS
  In conclusion, this report reveals lack of association between IL-8 - 251 A/T polymorphism and NSCLC in our Kashmir Valley population.
authors.affiliation: Department of Immunology and Molecular Medicine, Sher i Kashmir institute of Medical Sciences Srinagar, India. imty82@gmail.com
authors.forename: Imtiyaz Ahmad
authors.initials: IA
authors.lastname: Bhat
authors.forename: Arshid A
authors.initials: AA
authors.lastname: Pandith
authors.forename: Bashir A
authors.initials: BA
authors.lastname: Bhat
authors.forename: Niyaz A
authors.initials: NA
authors.lastname: Naykoo
authors.forename: Iqbal
authors.initials: I
authors.lastname: Qasim
authors.forename: Roohi
authors.initials: R
authors.lastname: Rasool
authors.forename: Sheik Aejaz
authors.initials: SA
authors.lastname: Aziz
authors.forename: Zafar Amin
authors.initials: ZA
authors.lastname: Shah
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000235:genetics / Q000473:pathology; D018287:Carcinoma, Large Cell / Q000235:genetics / Q000473:pathology; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D005838:Genotype; D006801:Humans; D007194:India; D016209:Interleukin-8 / Q000235:genetics*; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011379:Prognosis
chemical_list: D020413:3' Untranslated Regions; D016209:Interleukin-8