title: Fabrication, characterization and antimicrobial activities of thymol-loaded zein nanoparticles stabilized by sodium caseinate-chitosan hydrochloride double layers.
abstract: Thymol-loaded zein nanoparticles stabilized with sodium caseinate (SC) and chitosan hydrochloride (CHC) were prepared and characterized. The SC stabilized nanoparticles had well-defined size range and negatively charged surface. Due to the presence of SC, the stabilized zein nanoparticles showed a shift of isoelectric point from 6.18 to 5.05, and had a desirable redispersibility in water at neutral pH after lyophilization. Coating with CHC onto the SC stabilized zein nanoparticles resulted in increased particle size, reversal of zeta potential value from negative to positive, and improved encapsulation efficiency. Both thymol-loaded zein nanoparticles and SC stabilized zein nanoparticles had a spherical shape and smooth surface, while the surfaces of CHC-SC stabilized zein nanoparticles seemed rough and had some clumps. Encapsulated thymol was more effective in suppressing gram-positive bacterium than un-encapsulated thymol for a longer time period.
authors.affiliation: Institute of Food and Nutraceutical Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yuge
authors.initials: Y
authors.lastname: Niu
authors.forename: Yangchao
authors.initials: Y
authors.lastname: Luo
authors.forename: Mei
authors.initials: M
authors.lastname: Ge
authors.forename: Tian
authors.initials: T
authors.lastname: Yang
authors.forename: Liangli Lucy
authors.initials: LL
authors.lastname: Yu
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002364:Caseins / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D013943:Thymol / Q000737:chemistry* / Q000494:pharmacology; D015028:Zein / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D002364:Caseins; D004337:Drug Carriers; D013943:Thymol; D015028:Zein; D048271:Chitosan
keywords: Antimicrobial activity; Chitosan hydrochloride; Nanoparticle; Sodium caseinate; Thymol; Zein