title: Potential barriers to human hepatocyte transplantation in MUP-uPAtg(⁺/⁺)Rag2⁻/⁻γC⁻/⁻ mice.
abstract: Primary human fetal and adult hepatocytes have been considered feasible donor cell sources for cell transplantation. We compared the engraftment efficiencies between adult human, fetal human, and adult porcine hepatocytes after transplantation into MUP-uPA(tg(+/+))Rag2(-/-)γC(-/-)mice. Transplantation of adult human hepatocytes yielded a 1,000-fold higher serum albumin level compared to transplantation of fetal human hepatocytes, while transplantation of adult porcine hepatocytes resulted in a 100-fold higher serum albumin level than adult human hepatocytes. These results suggest that adult liver cells are superior to fetal liver cells for transplantation, and caution should be applied if porcine hepatocytes are used for preclinical studies as a proof of concept for human hepatocytes.
authors.affiliation: McGowan Institute for Regenerative Medicine, Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
authors.forename: Junji
authors.initials: J
authors.lastname: Komori
authors.forename: Aaron D
authors.initials: AD
authors.lastname: DeWard
authors.forename: Roberto
authors.initials: R
authors.lastname: Gramignoli
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Strom
authors.forename: Paulo
authors.initials: P
authors.lastname: Fontes
authors.forename: Eric
authors.initials: E
authors.lastname: Lagasse
mesh_terms: D000328:Adult; D000818:Animals; D004268:DNA-Binding Proteins / Q000172:deficiency* / Q000378:metabolism; D022781:Hepatocytes / Q000637:transplantation*; D006801:Humans; D053631:Interleukin Receptor Common gamma Subunit / Q000172:deficiency* / Q000378:metabolism; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011506:Proteins / Q000378:metabolism*; D034421:Sus scrofa; D014183:Transplantation, Heterologous; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D053631:Interleukin Receptor Common gamma Subunit; D011506:Proteins; C499308:Rag2 protein, mouse; C035737:major urinary proteins; D014568:Urokinase-Type Plasminogen Activator