title: [Efficacy of cyclosporine A-nanoparticles emulsion combined with stem cell transplantation therapy for acute myocardial infarction].
abstract: OBJECTIVE
  To evaluate the efficacy of cyclosporine A-nanoparticles emulsion (CsA-NP) combined with adipose tissue-derived stem cells (ASCs)transplantation therapy for acute myocardial infarction (AMI) in a miniswine model.
  
  
  METHODS
  CsA-NP emulsion was prepared by the high-pressure homogenization method. Models were performed by coronary angioplasty for percutaneous balloon occlusion of left anterior descending artery (LAD). A total of 17 miniswines survived after AMI were divided into four groups: control group (n=5), CsA-NP group (n=4), ASCs group (n=4), and CsA-NP+ASCs group (n=4). ASCs or saline were delivered by intracoronary injection one week after AMI.Before cell transplantation and 8 weeks after cell transplantation, delayed-enhanced magnetic resonance imaging (DE-MRI) was performed to evaluate cardiac function and viability. The infarcted myocardium and implanted cells were histologically studied.
  
  
  RESULTS
  Eight weeks after treatment, the left ventricular ejection fraction (LVEF)significantly increased in the CsA-NP+ASCs group when compared with the ASCs group [(53.6 ± 2.4)% vs. (48.3 ± 1.8)%, P<0.05]; meanwhile, the infarct size significantly decreased [(6.2 ± 1.7)cm(3) vs.(7.5 ± 0.6) cm(3), P<0.05] and the thickness of the ventricular wall significantly increased (P<0.05). Histology showed that the number of surviving cells increased nearly by three times in the CsA-NP+ASCs group, and the expressions of the cardiomyocyte specific markers (cTnT and α-actin) were detected. Histological samples also showed that CsA-NP+ASCs group reduced fibrotic tissue, and down-regulated the activation of Caspase-3.
  
  
  CONCLUSIONS
  The CsA-NP+ASCs combination therapy can enhance the viability of ASCs by improving LVEF and preventing LV expansion, which may be explained that CsA-NP has the anti-apoptotic effect and can promote the survivals and proliferation of ASCs.
authors.affiliation: Department of Geriatric Cardiology, Chinese General Hospital of Air Force, Beijing, China.
authors.forename: Qiao-xiang
authors.initials: QX
authors.lastname: Yin
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Pei
authors.forename: Yu-sheng
authors.initials: YS
authors.lastname: Zhao
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D016572:Cyclosporine / Q000627:therapeutic use*; D004195:Disease Models, Animal; D009203:Myocardial Infarction / Q000628:therapy*; D053758:Nanoparticles; D011897:Random Allocation; D033581:Stem Cell Transplantation; D013552:Swine
chemical_list: D016572:Cyclosporine; D053148:Caspase 3