title: Effect of blood handling conditions on progesterone assay results obtained by chemiluminescence in the bitch.
abstract: Assay of blood progesterone (P4) is commonly practiced to determine the time of ovulation, diagnose luteal insufficiency, and predict time of parturition in bitches. Because of practical constraints, most blood samples cannot be assayed on site immediately after collection. The aim of this work was to evaluate the effects of various sampling and storage conditions on concentrations of P4 as determined by chemiluminescence immunoassay. The blood of 5 Beagle bitches was collected from the jugular vein to study the effect of the type of collection tube (silicone, lithium heparin, EDTA), the storage time of unseparated or separated plasma (2 h to 14 d), and the number of freeze-thaw cycles (1-10) on P4. The effect of each factor was tested within one assay session. None of the factors significantly affected P4. Thus, P4 appears to remain relatively stable in canine blood samples exposed to various processing and storage conditions.
authors.affiliation: INRA, UMR 1198 Biologie du Développement et Reproduction, 78350 Jouy en Josas, France; ENVA, Université Paris Est, 7 avenue du Général de Gaulle, 94704 Maisons-Alfort, France.
authors.forename: M Z
authors.initials: MZ
authors.lastname: Tahir
authors.forename: S
authors.initials: S
authors.lastname: Thoumire
authors.forename: M
authors.initials: M
authors.lastname: Raffaelli
authors.forename: B
authors.initials: B
authors.lastname: Grimard
authors.forename: K
authors.initials: K
authors.lastname: Reynaud
authors.forename: S
authors.initials: S
authors.lastname: Chastant-Maillard
mesh_terms: D000818:Animals; D000925:Anticoagulants; D001793:Blood Preservation / Q000662:veterinary; D001800:Blood Specimen Collection / Q000295:instrumentation / Q000379:methods / Q000662:veterinary; D015925:Cryopreservation / Q000662:veterinary; D004285:Dogs / Q000097:blood*; D004355:Drug Stability; D005260:Female; D007118:Immunoassay / Q000379:methods / Q000662:veterinary; D008163:Luminescent Measurements / Q000662:veterinary; D011374:Progesterone / Q000097:blood*; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors
chemical_list: D000925:Anticoagulants; D011374:Progesterone
keywords: Anticoagulant; Canine; Chemiluminescence; Freeze-thaw cycles; Progesterone; Storage time