title: The immunostimulatory effect of novel immunostimulator CH2b with a thiazolidin-4-one ring on the functions of LPS-activated RAW 264.7 macrophages in vitro.
abstract: This study was designed to confirm the effect of the novel immunostimulators CH1b and CH2b with a thiazolidin-4-one ring on the function of macrophages. We used these two molecules to stimulate LPS-activated RAW 264.7 macrophages in vitro. After a series of essential assays, we found that CH1b and CH2b could significantly increase the production of nitric oxide (NO) by the LPS-activated RAW 264.7 macrophages, and also found that CH2b could more significantly increase the proliferation, phagocytic activity, and secretion of IL-6, IL-8 and TNF-α by LPS-activated RAW 264.7 cells more than CH1b. Furthermore, CH2b could increase the degradation of IκB-α and could promote the nuclear translocation of nuclear factor (NF)-κB p65 in LPS-activated RAW 264.7 cells. However, CH2b could also increase the phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK). Taken together, we got that CH2b could further increase the LPS-induced activation of NF-κB and p38 MAPK in RAW 264.7 macrophages to elevate the function of macrophages, including iNOS expression, NO production, cytokines secretion, and phagocytosis.
authors.affiliation: Department of Immunology, School of Medicine, Hebei University, Baoding 071002, China.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Meng
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Chen
authors.forename: Zerui
authors.initials: Z
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Han
authors.forename: Hua
authors.initials: H
authors.lastname: Chen
authors.forename: Jinchao
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiaoliu
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D007155:Immunologic Factors / Q000494:pharmacology*; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D009705:Nucleosides / Q000494:pharmacology*; D053778:Thiazolidines / Q000494:pharmacology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D016207:Cytokines; D007155:Immunologic Factors; D008070:Lipopolysaccharides; D016328:NF-kappa B; D009705:Nucleosides; D053778:Thiazolidines; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Immunostimulator; NF-κB; RAW 264.7; iNOS; p38 MAPK