title: Archaea dominate ammonia oxidizers in the permian water ecosystem of midland basin.
abstract: We investigated the existence and characteristics of ammonia oxidizers in Permian water from Midland Basin. Molecular surveys targeting the amoA gene showed that only ammonia-oxidizing archaea (AOA) exist and have potential activity in this special environment. In contrast, no ammonia-oxidizing bacteria (AOB) were detected in the water. Phylogenetic analysis indicated that 72-89% of the total screened AOA clones were affiliated with those found in underground water, and 10-24% of the AOA clones were related to those found in marine water or sediments. Our results indicate AOA might be the most abundant ammonia-oxidizing microbes in this ecological niche.
authors.affiliation: State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences.
authors.forename: Yiguo
authors.initials: Y
authors.lastname: Hong
authors.forename: Wang
authors.initials: W
authors.lastname: Youshao
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D001105:Archaea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D044822:Biodiversity; D017753:Ecosystem; D019015:Geologic Sediments / Q000382:microbiology; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010802:Phylogeny; D012623:Seawater / Q000382:microbiology*
chemical_list: D000641:Ammonia