title: Comprehensive analysis of the transcriptional landscape of the human FMR1 gene reveals two new long noncoding RNAs differentially expressed in Fragile X syndrome and Fragile X-associated tremor/ataxia syndrome.
abstract: The majority of the human genome is transcribed but not translated, giving rise to noncoding RNAs (ncRNAs), including long ncRNAs (lncRNAs, >200 nt) that perform a wide range of functions in gene regulation. The Fragile X mental retardation 1 (FMR1) gene is a microsatellite locus that in the general population contains <55 CGG repeats in its 5'-untranslated region. Expansion of this repeat region to a size of 55-200 CGG repeats, known as premutation, is associated with Fragile X tremor and ataxia syndrome (FXTAS). Further expansion beyond 200 CGG repeats, or full mutation, leads to FMR1 gene silencing and results in Fragile X syndrome (FXS). Using a novel technology called "Deep-RACE", which combines rapid amplification of cDNA ends (RACE) with next generation sequencing, we systematically interrogated the FMR1 gene locus for the occurrence of novel lncRNAs. We discovered two transcripts, FMR5 and FMR6. FMR5 is a sense lncRNA transcribed upstream of the FMR1 promoter, whereas FMR6 is an antisense transcript overlapping the 3' region of FMR1. FMR5 was expressed in several human brain regions from unaffected individuals and from full and premutation patients. FMR6 was silenced in full mutation and, unexpectedly, in premutation carriers suggesting abnormal transcription and/or chromatin remodeling prior to transition to the full mutation. These lncRNAs may thus be useful as biomarkers, allowing for early detection and therapeutic intervention in FXS and FXTAS. Finally we show that FMR5 and FMR6 are expressed in peripheral blood leukocytes and propose future studies that correlate lncRNA expression with clinical outcomes.
authors.affiliation: Department of Psychiatry and Behavioral Sciences and Center for Therapeutic Innovation, Hussman Institute for Human Genomics, University of Miami, Miller School of Medicine, Miami, FL, 33136, USA, cpastori@med.miami.edu.
authors.forename: Chiara
authors.initials: C
authors.lastname: Pastori
authors.forename: Veronica J
authors.initials: VJ
authors.lastname: Peschansky
authors.forename: Deborah
authors.initials: D
authors.lastname: Barbouth
authors.forename: Arpit
authors.initials: A
authors.lastname: Mehta
authors.forename: Jose P
authors.initials: JP
authors.lastname: Silva
authors.forename: Claes
authors.initials: C
authors.lastname: Wahlestedt
mesh_terms: D020121:5' Untranslated Regions; D001259:Ataxia / Q000235:genetics*; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000235:genetics* / Q000378:metabolism; D005600:Fragile X Syndrome / Q000235:genetics*; D005786:Gene Expression Regulation; D056426:Genetic Loci; D006579:Heterozygote; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D009154:Mutation; D011401:Promoter Regions, Genetic; D062085:RNA, Long Noncoding / Q000235:genetics / Q000302:isolation & purification*; D014202:Tremor / Q000235:genetics*; D019680:Trinucleotide Repeat Expansion
chemical_list: D020121:5' Untranslated Regions; D051860:Fragile X Messenger Ribonucleoprotein 1; D062085:RNA, Long Noncoding; C497683:FMR1 protein, human; C000613324:long non-coding RNA FMR6, human