title: Epstein-barr virus latent membrane protein 1 polymorphism in nasopharyngeal carcinoma and other oral cavity tumors in Russia.
abstract: The genetic structure of EBV LMP1 alleles isolated from tumor, blood, and throat washing samples of 22 nasopharyngeal carcinoma patients, 17 patients with other non-EBV-related tumors of the oral cavity, and 19 blood donors have been studied in representatives of Central Russia and the Republics of Northern Caucasus, regions which are non-endemic for nasopharyngeal carcinoma. The analysis of the LMP1 alleles collected revealed that they practically matched previously described LMP1 variants; however, some characteristic features were also detected. In particular, the G212S substitution in LMP1 isolates investigated was not observed at all. Tumor samples obtained from nasopharyngeal carcinoma and other tumors of the oral cavity did not differ significantly either in the frequency of "high oncogenic" LMP1 alleles with 10 aa and/or 23 aa deletions (LMP1(China1) and/or LMP1(Med+)), nor in the number of 11 aa repeats and the frequency of 5 aa motif insertions. No differences in the frequency of amino acid substitutions between LMP1 alleles obtained from tumor and throat washing samples of both patient groups were also detected. The data obtained may indicate that in both nasopharyngeal carcinoma patients and patients with other tumors of the oral cavity, the EBV strains with similar LMP1 variants are found to persist. This observation allows us to suggest that in non-endemic areas, EBV strains with any LMP1 alleles can initiate the nasopharyngeal carcinoma development but only in those individuals who have a genetic predisposition to the disease and are subjected to specific environmental, and/or dietary factors present in certain geographic areas.
authors.affiliation: Laboratory of Viral Carcinogenicity, N.N. Blokhin Cancer Research Center, Moscow, Russia.
authors.forename: Natalia
authors.initials: N
authors.lastname: Senyuta
authors.forename: Larissa
authors.initials: L
authors.lastname: Yakovleva
authors.forename: Elena
authors.initials: E
authors.lastname: Goncharova
authors.forename: Liana
authors.initials: L
authors.lastname: Scherback
authors.forename: Sergey
authors.initials: S
authors.lastname: Diduk
authors.forename: Ksenia
authors.initials: K
authors.lastname: Smirnova
authors.forename: Dmitry
authors.initials: D
authors.lastname: Maksimovich
authors.forename: Vladimir
authors.initials: V
authors.lastname: Gurtsevitch
mesh_terms: D000595:Amino Acid Sequence; D001782:Blood Donors; D002277:Carcinoma; D016000:Cluster Analysis; D005260:Female; D014644:Genetic Variation; D064368:Healthy Volunteers; D004854:Herpesvirus 4, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009062:Mouth Neoplasms / Q000821:virology*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000821:virology*; D010802:Phylogeny; D012426:Russia; D016415:Sequence Alignment; D014763:Viral Matrix Proteins / Q000235:genetics*
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins
keywords: Epstein-Barr virus; LMP1 sequence variations; Russia; phylogenetic tree