title: High-performance liquid chromatographic determination of Ethamsylate in urine and tablets.
abstract: A simple, selective, sensitive, accurate, and precise method was developed for determination of ethamsylate (ET) in human urine and in ET tablets using RP-HPLC. The method uses a C18 (5 pm particle size) column at ambient temperature with the mobile phase 14.7 mM potassium dihydrogen phosphate (pH 4.6)-8.15 mM tetraheptylammonium bromide in acetonitrile (50 + 50, v/v) at a flow rate of 1.0 mL/min. Quantitation was achieved with UV detection at 300 nm, based on peak area with a linear calibration curve in the concentration range of 0.1-100 microg/mL. The proposed method was applied for the determination of the urinary excretion pattern of ET as the cumulative amounts excreted have been calculated without pretreatment of urine samples. The proposed method was completely validated according to U.S. Food and Drug administration guidelines.
authors.affiliation: Suez Canal University, Faculty of Pharmacy, Analytical Chemistry Department, Ismailia, Egypt.
authors.forename: Alaa
authors.initials: A
authors.lastname: El-Gindy
authors.forename: Mohammad Wafaa
authors.initials: MW
authors.lastname: Nassar
authors.forename: Khalid Abdel-Salam
authors.initials: KA
authors.lastname: Attia
authors.forename: Hamed Hamed
authors.initials: HH
authors.lastname: Abu-Seada
authors.forename: Mahmoud
authors.initials: M
authors.lastname: El-Ghandour
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004979:Ethamsylate / Q000032:analysis* / Q000652:urine; D006490:Hemostatics / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008297:Male; D015203:Reproducibility of Results; D013607:Tablets / Q000737:chemistry
chemical_list: D006490:Hemostatics; D013607:Tablets; D004979:Ethamsylate