title: Raddeanin A induces human gastric cancer cells apoptosis and inhibits their invasion in vitro.
abstract: Raddeanin A is one of the triterpenoid saponins in herbal medicine Anemone raddeana Regel which was reported to suppress the growth of liver and lung cancer cells. However, little was known about its effect on gastric cancer (GC) cells. This study aimed to investigate its inhibitory effect on three kinds of different differentiation stage GC cells (BGC-823, SGC-7901 and MKN-28) in vitro and the possible mechanisms. Proliferation assay and flow cytometry demonstrated Raddeanin A's dose-dependent inhibitory effect and determined its induction of cells apoptosis, respectively. Transwell assay, wounding heal assay and cell matrix adhesion assay showed that Raddeanin A significantly inhibited the abilities of the invasion, migration and adhesion of the BGC-823 cells. Moreover, quantitative real time PCR and Western blot analysis found that Raddeanin A increased Bax expression while reduced Bcl-2, Bcl-xL and Survivin expressions and significantly activated caspase-3, caspase-8, caspase-9 and poly-ADP ribose polymerase (PARP). Besides, Raddeanin A could also up-regulate the expression of reversion inducing cysteine rich protein with Kazal motifs (RECK), E-cadherin (E-cad) and down-regulate the expression of matrix metalloproteinases-2 (MMP-2), MMP-9, MMP-14 and Rhoc. In conclusion, Raddeanin A inhibits proliferation of human GC cells, induces their apoptosis and inhibits the abilities of invasion, migration and adhesion, exhibiting potential to become antitumor drug.
authors.affiliation: Department of Oncology, Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Xue
authors.forename: Xi
authors.initials: X
authors.lastname: Zou
authors.forename: Jin-Yong
authors.initials: JY
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.forename: Jia-Li
authors.initials: JL
authors.lastname: Xu
authors.forename: Rui-Ping
authors.initials: RP
authors.lastname: Wang
mesh_terms: D031883:Anemone / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005753:Gastric Mucosa / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D012333:RNA, Messenger / Q000235:genetics; D012503:Saponins / Q000737:chemistry / Q000494:pharmacology*; D013270:Stomach / Q000187:drug effects / Q000473:pathology; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D012333:RNA, Messenger; D012503:Saponins; C584473:raddeanin A
keywords: Adhesion; Apoptosis; E-cad; E-cadherin; GC; Gastric cancer cell; Invasion; MMP; OD; PARP; RECK; Raddeanin A; gastric cancer; matrix metalloproteinase; optical density; poly-ADP ribose polymerase; reversion inducing cysteine rich protein with Kazal motifs