title: Polyamidoamine dendrimer conjugated chitosan nanoparticles for the delivery of methotrexate.
abstract: Encapsulating anticancer drugs to synthetic polymer is a promising approach to improve the efficiency and reduce the side effects of anticancer drugs. In this study, novel chitosan derivatives with polyamidoamine moieties (CS-PAMAM) were synthesized and characterized by morphology, particle size, and zeta potential. Then the anticancer drug-methotrexate-encapsulated CS-PAMAM was prepared by hydrophobic-hydrophilic interactions. The drug release assay showed that the amount of the methotrexate release from CS-PAMAM was pH depended. Meanwhile, the cell viability assay illustrated that CS-PAMAM was suitable for the drug delivery because of its low cytotoxicity on cells. Moreover, our results showed that the CS-PAMAM could significantly improve the cytotoxicity of free methotrexate on A549 cells. These results demonstrate that CS-PAMAM may provide a suitable platform for the water-insoluble drug delivery.
authors.affiliation: School of Chemistry and Materials Science, Guizhou Normal University, Guiyang, Guizhou, China.
authors.forename: Zhen-Hua
authors.initials: ZH
authors.lastname: Leng
authors.forename: Qian-Fen
authors.initials: QF
authors.lastname: Zhuang
authors.forename: Yan-Chao
authors.initials: YC
authors.lastname: Li
authors.forename: Zeng
authors.initials: Z
authors.lastname: He
authors.forename: Zhao
authors.initials: Z
authors.lastname: Chen
authors.forename: Sai-Peng
authors.initials: SP
authors.lastname: Huang
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Jia
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Zhou
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Li-Bo
authors.initials: LB
authors.lastname: Du
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry*; D001692:Biological Transport; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D050091:Dendrimers / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D042541:Intracellular Space / Q000378:metabolism; D008727:Methotrexate / Q000737:chemistry*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D010974:Platelet Aggregation / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D050091:Dendrimers; D004337:Drug Carriers; C104700:PAMAM Starburst; D048271:Chitosan; D016650:Fluorescein-5-isothiocyanate; D008727:Methotrexate
keywords: Chitosan; Drug delivery; Methotrexate; Nanoparticles; PAMAM