title: Production of the Acetylcholinesterase Inhibitor from Yarrowia lipolytica S-3.
abstract: The acetylcholinesterase (AChE) inhibitor of Yarrowia lipolytica S-3 was maximally produced when it was incubated at 30℃ for 36 h in an optimal medium containing 1% yeast extract, 2% peptone and 2% glucose, with an initial pH 6.0. The final AChE inhibitory activity under these conditions was an IC50 value of 64 mg/ml. After partial purification of the AChE inhibitor by means of systematic solvent extraction, the final IC50 value of the partially purified AChE inhibitor was 0.75 mg/ml. We prepared a test product by using the partially purified AChE inhibitor and then determined its stability for the development of a new antidementia commercial product. The test product was stable at room temperature for 15 weeks.
authors.affiliation: Department of Life Science and Genetic Engineering, Paichai University, Daejeon 302-735, Korea.
authors.forename: Dae-Hyung
authors.initials: DH
authors.lastname: Lee
authors.forename: Ji-Su
authors.initials: JS
authors.lastname: Lee
authors.forename: Sung-Hun
authors.initials: SH
authors.lastname: Yi
authors.forename: Jong-Soo
authors.initials: JS
authors.lastname: Lee
keywords: Acetylcholinesterase inhibitor; Antidementia; Yarrowia lipolytica S-3