title: Extracellular Ca(2+) and Mg(2+) modulate aminoglycoside blockade of mechanotransducer channel-mediated Ca(2+) entry in zebrafish hair cells: an in vivo study with the SIET.
abstract: Zebrafish lateral-line hair cells are an in vivo model for studying hair cell development, function, and ototoxicity. However, the molecular identification and properties of the mechanotransducer (MET) channel in hair cells are still controversial. In this study, a noninvasive electrophysiological method, the scanning ion-electrode technique (SIET), was applied for the first time to investigate properties of MET channels in intact zebrafish embryos. With the use of a Ca(2+)-selective microelectrode to deflect hair bundles and simultaneously record the Ca(2+) flux, the inward Ca(2+) flux was detected at stereocilia of hair cells in 2- to ~4-day postfertilization embryos. Ca(2+) influx was blocked by MET channel blockers (BAPTA, La(3+), Gd(3+), and curare). In addition, 10 μM aminoglycoside antibiotics (neomycin and gentamicin) were found to effectively block Ca(2+) influx within 10 min. Elevating the external Ca(2+) level (0.2-2 mM) neutralized the effects of neomycin and gentamicin. However, elevating the Mg(2+) level up to 5 mM neutralized blockade by gentamicin but not by neomycin. This study demonstrated MET channel-mediated Ca(2+) entry at hair cells and showed that the SIET to be a sensitive approach for functionally assaying MET channels in zebrafish.
authors.affiliation: Department of Life Science, National Taiwan Normal University, Taipei, Taiwan, Republic of China;
authors.forename: Li-Yih
authors.initials: LY
authors.lastname: Lin
authors.forename: Wei
authors.initials: W
authors.lastname: Pang
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Chuang
authors.forename: Giun-Yi
authors.initials: GY
authors.lastname: Hung
authors.forename: Yuan-Hsiang
authors.initials: YH
authors.lastname: Lin
authors.forename: Jiun-Lin
authors.initials: JL
authors.lastname: Horng
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005839:Gentamicins / Q000494:pharmacology*; D006198:Hair Cells, Auditory / Q000187:drug effects* / Q000378:metabolism; D017736:Ion-Selective Electrodes; D008274:Magnesium / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D040542:Mechanotransduction, Cellular / Q000502:physiology; D009355:Neomycin / Q000494:pharmacology*; D015027:Zebrafish
chemical_list: D000617:Aminoglycosides; D005839:Gentamicins; D009355:Neomycin; D008274:Magnesium; D002118:Calcium
keywords: MET channel; SIET; aminoglycoside; hair cell; neuromast