title: Ontogeny of the avian intestinal immunoglobulin repertoire: modification in CDR3 length and conserved VH-pseudogene usage.
abstract: Immunoglobulins play an important role in maintenance of mucosal homeostasis in the gut. The antigen binding specificity of these immunoglobulins depends for a large part on the hypervariable CDR3 region. To gain knowledge about isotype-specific development of the CDR3 repertoire we examined CDR3 spectratypes at multiple time points between 4 and 70 days post hatch. In order to identify clonal expansions deviation from the normal distribution (SS) and the average CDR3 length was calculated. IgA-CDR3 regions were studied in more detail by DNA sequence analysis at day 7 and 70 and preferential VH pseudogene usage was estimated. The SS of CDR3 repertoires of the IgM, IgG and IgA isotypes successively increased, but for each isotype this increase was transiently. The length of the CDR3 regions decreased with age for IgM becoming similar to the CDR3 length of IgA at day 70. The IgA- and IgG-CDR3 lengths did not change with age. On average, the CDR3 length of IgA was the shortest. IgA CDR3 sequences were similar between animals aged 7 and 70 days. A limited number of pseudogenes was used, and no differences in pseudogene usage were observed between animals aged 7 and 70 days. Of the identified VH pseudogenes, half of the sequences used VH15, whilst a number of the pseudogenes were not used at all. We conclude that CDR3 spectratype profiles change during aging, whilst at the CDR3-sequence level, variation in VH pseudogene usage for ileal IgA is limited suggesting conservation during ontogeny.
authors.affiliation: Cell Biology and Immunology Group, Wageningen University, The Netherlands; Adaptation Physiology Group, Wageningen University, The Netherlands. Electronic address: cdenhartog@ucsd.edu.
authors.forename: Gerco
authors.initials: G
authors.lastname: den Hartog
authors.forename: Richard P M A
authors.initials: RP
authors.lastname: Crooijmans
authors.forename: Henk K
authors.initials: HK
authors.lastname: Parmentier
authors.forename: Huub F J
authors.initials: HF
authors.lastname: Savelkoul
authors.forename: Nicolaas A
authors.initials: NA
authors.lastname: Bos
authors.forename: Aart
authors.initials: A
authors.lastname: Lammers
mesh_terms: D000818:Animals; D000916:Antibody Diversity / Q000235:genetics; D030161:Avian Proteins / Q000145:classification / Q000235:genetics*; D002642:Chick Embryo; D002645:Chickens; D016000:Cluster Analysis; D022801:Complementarity Determining Regions / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000235:genetics* / Q000276:immunology; D007070:Immunoglobulin A / Q000235:genetics; D007074:Immunoglobulin G / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007075:Immunoglobulin M / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007136:Immunoglobulins / Q000145:classification / Q000235:genetics*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000254:growth & development / Q000276:immunology; D011544:Pseudogenes / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D030161:Avian Proteins; D022801:Complementarity Determining Regions; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region; D007136:Immunoglobulins
keywords: Avian/chicken; CDR3 repertoire; IgA; Immunoglobulin; Intestine; VH pseudogene