title: [Adipose tissue-derived adult stem cells transfected with the gene of hyperpolarization-activated cyclic nucleotide-gated ion channel 2 differentiated into pacemaker-like cells].
abstract: OBJECTIVE
  To transfect rat adipose tissue-derived adult stem cells (ADSCs) with the pacemaker gene of human hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (hHCN2), and observe its feasibility of functioning as pacemaker cells.
  
  
  METHODS
  First of all, healthy ADSCs were harvested and transfected with hHCN2 gene. Thereafter, we detected the expression of hHCN2 gene and protein by quantitative real-time PCR (qRT-PCR), Western blot analysis and immunofluorescence cytochemistry and measured the electrophysiological properties and recorded the inward currents using patch clamp techniques. In addition, we mixed and co-cultured the transfected ADSCs and rat cadiomyocytes to observe the influence on the spontaneous beating rate of cadiomyocytes.
  
  
  RESULTS
  Compared with the control ADSCs, the transfected ADSCs expressed the higher level of hHCN2 gene and protein(P<0.05), and they could produce pacemaker current. When co-cultured with rat ventricular myocytes, they could accelerate the spontaneous beating rate of the ventricular myocytes(P<0.05).
  
  
  CONCLUSIONS
  After transfected with hHCN2 gene, ADSCs can work as pacemaker-like cells, which provides materials for the research of biological pacemakers.
authors.affiliation: Department of Cardiology, Jiangxi Institute of Hypertensive Diseases, Nanchang, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Bingong
authors.initials: B
authors.lastname: Li
authors.forename: Zhe
authors.initials: Z
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Minghui
authors.initials: M
authors.lastname: Zeng
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D053687:Adult Stem Cells / Q000378:metabolism*; D000818:Animals; D001683:Biological Clocks; D002454:Cell Differentiation; D016764:Cell Polarity; D005260:Female; D005455:Fluorescent Antibody Technique; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D007473:Ion Channels / Q000235:genetics*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection
chemical_list: D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D007473:Ion Channels