title: A colormetric and fluorescent chemosensor for adenosine-5'-triphosphate based on rhodamine derivative.
abstract: A rhodamine spirolactam derivative (1) was developed as a colormetric and fluorescent chemosensor for adenosine-5'-triphosphate (ATP) via hydrogen bonds interaction. As far as we know, this is the first case to explore ATP-induced ring-opening of spirolactam in rhodamine derivatives. It exhibited a highly sensitive "turn-on" fluorescent response toward ATP with a 47-fold fluorescence intensity enhancement under 20 equiv. of ATP added. The chemosensor can be applied to the quantification of ATP with a linear range covering from 1.0×10(-7) to 2.0×10(-4) M and a detection limit of 2.5×10(-8) M. The experiment results show that the response behavior of 1 toward ATP is pH independent in medium condition (pH 6.0-8.0). Most importantly, the novel chemosensor has well solved the problem of serious interferences from other nucleoside polyphosphates such as ADP and AMP generally met by previously reported typical fluorescent chemosensors for ATP. Moreover, the response of the chemosensor toward ATP is fast (response time less than 3 min). In addition, the chemosensor can be used for the fluorescence assay for protein kinase activity with satisfactory results. The chemosensor for ATP based on hydrogen bonds interaction provided a novel strategy for the design of colormetric and ratiometric fluorescent probes for other target anions with high sensitivity and selectivity.
authors.affiliation: Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, PR China. lichunyan79@sina.com
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Li
authors.forename: Chun-Xiang
authors.initials: CX
authors.lastname: Zou
authors.forename: Yong-Fei
authors.initials: YF
authors.lastname: Li
authors.forename: Xue-Fei
authors.initials: XF
authors.lastname: Kong
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.forename: Yin-Shuang
authors.initials: YS
authors.lastname: Wu
authors.forename: Wei-Guo
authors.initials: WG
authors.lastname: Zhu
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012235:Rhodamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D000255:Adenosine Triphosphate; D017868:Cyclic AMP-Dependent Protein Kinases
keywords: Adenosine-5′-triphosphate; Chemosensor; Fluorescence; Rhodamine