title: Immuno-fluorescence staining patterns of leukocyte subsets in the skin of taurine and indicine cattle.
abstract: The immuno-staining patterns of skin leukocytes were investigated in three breeds of cattle: Holstein-Friesian, Brahman and Santa Gertrudis of similar age before and after tick infestation. The antibodies specific for CD45 and CD45RO reacted with cells in the skin of all Holstein-Friesian cattle but did not react with cells in the skin of any Brahman cattle. The same antibodies reacted with cells from the skin of four (CD45) and seven (CD45RO) of twelve Santa Gertrudis cattle. The antibodies specific for T cells and γδ subset of T cells recognized cells from all three breeds of cattle. The antibody specific for MHC class II molecules labelled cells of mostly irregular shape, presumably dermal dendritic cells and/or macrophages and Langerhans cells. The antibody specific for granulocytes (mAb CH138) reacted with cells only in sections cut from skin with lesions. The antibody specific for CD25(+) cells labelled regularly shaped cells that showed a wide range of intensities of staining.
authors.affiliation: Cooperative Research Centre for Beef Genetic Technologies, Armidale 2351, Australia; Queensland Government, Department of Employment Economic Development and Innovation (DEEDI) Yeerongpilly, Queensland 4105, Australia; School of Veterinary Science, The University of Queensland, Gatton, Queensland 4343, Australia. Electronic address: constantin.constantinoiu@jcu.edu.au.
authors.forename: C C
authors.initials: CC
authors.lastname: Constantinoiu
authors.forename: N N
authors.initials: NN
authors.lastname: Jonsson
authors.forename: W K
authors.initials: WK
authors.lastname: Jorgensen
authors.forename: L A
authors.initials: LA
authors.lastname: Jackson
authors.forename: E K
authors.initials: EK
authors.lastname: Piper
authors.forename: A E
authors.initials: AE
authors.lastname: Lew-Tabor
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002417:Cattle; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D007962:Leukocytes / Q000166:cytology* / Q000276:immunology; D012867:Skin / Q000166:cytology* / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes
keywords: Breed; Cattle; Immuno-fluorescence; Leukocyte; Skin