title: Using protein nanofibrils to remove azo dyes from aqueous solution by the coagulation process.
abstract: The ever-increasing applications of hazardous azo dyes as industrialized coloring agents have led to serious remediation challenges. In this study, proteinaceous nanofibrils were examined as coagulants for decolorization of azo dyes in aqueous solutions. The results provided some insight regarding the mechanism of dye removal. The strength of nanofibrils to remove dyes from solution was evaluated by remediation of acid red 88, Bismarck brown R, direct violet 51, reactive black 5, and Congo red. However, the efficiency of nanofibrils to coagulate with different dyes was variable (60-98%) and dependent on the structures of dyes and the physicochemical conditions of the solutions. Increasing the temperature or ionic strength declined the coagulation time and induced the rate of dye removal. Changing pH had contradictory effects on the dye removal efficiency which was more affected by the chemical structure of the dye rather than the change in stability of the coagulant. The efficiency of nanofibrils to remove dyes was more than that of charcoal, which is considered as one of the most common substances used for azo dye remediation which may be due to its well dispersion in the aqueous solutions, and slower rates of the coagulation than that of the adsorption process. Furthermore, cytotoxicity was not detected after treating cell cultures with the decolorized solutions. Accordingly, by integrating biological and biophysicochemical processes, proteinaceous nanofibrils can be promising candidates for treatment of colored wastewaters. Ease of production, proper and quick dispersion in water, without the production of dangerous dye by-products and derivatives, are some of the main advantages of nanofibrils.
authors.affiliation: Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran. Electronic address: morshedi@nigeb.ac.ir.
authors.forename: Dina
authors.initials: D
authors.lastname: Morshedi
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Mohammadi
authors.forename: Masoud Mashhadi
authors.initials: MM
authors.lastname: Akbar Boojar
authors.forename: Farhang
authors.initials: F
authors.lastname: Aliakbari
mesh_terms: D000327:Adsorption; D000818:Animals; D030161:Avian Proteins / Q000737:chemistry; D001391:Azo Compounds / Q000302:isolation & purification* / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002606:Charcoal; D004396:Coloring Agents / Q000302:isolation & purification* / Q000633:toxicity; D052918:Environmental Restoration and Remediation / Q000379:methods*; D056945:Hep G2 Cells; D006801:Humans; D009113:Muramidase / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011506:Proteins / Q000737:chemistry*; D012996:Solutions; D062065:Wastewater / Q000737:chemistry; D014867:Water
chemical_list: D030161:Avian Proteins; D001391:Azo Compounds; D002606:Charcoal; D004396:Coloring Agents; D009113:Muramidase; D011506:Proteins; D012996:Solutions; D062065:Wastewater; D014867:Water; C421864:hen egg lysozyme
keywords: Azo dyes; Coagulation process; Dye removal; Proteinaceous nanofibrils