title: Fatty alcohols for wax esters in Marinobacter aquaeolei VT8: two optional routes in the wax biosynthesis pathway.
abstract: The biosynthesis of wax esters in bacteria is accomplished by a unique pathway that combines a fatty alcohol and a fatty acyl coenzyme A substrate. Previous in vitro enzymatic studies indicated that two different enzymes could be involved in the synthesis of the required fatty alcohol in Marinobacter aquaeolei VT8. In this study, we demonstrate through a series of gene deletions and transcriptional analysis that either enzyme is capable of fulfilling the role of providing the fatty alcohol required for wax ester biosynthesis in vivo, but evolution has clearly selected one of these, a previously characterized fatty aldehyde reductase, as the preferred enzyme to perform this reaction under typical wax ester-accumulating conditions. These results complement previous in vitro studies and provide the first glimpse into the role of each enzyme in vivo in the native organism.
authors.affiliation: Department of Bioproducts and Biosystems Engineering.
authors.forename: Eric M
authors.initials: EM
authors.lastname: Lenneman
authors.forename: Janet M
authors.initials: JM
authors.lastname: Ohlert
authors.forename: Nagendra P
authors.initials: NP
authors.lastname: Palani
authors.forename: Brett M
authors.initials: BM
authors.lastname: Barney
mesh_terms: D000217:Acyltransferases / Q000378:metabolism; D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004952:Esters / Q000737:chemistry; D019143:Evolution, Molecular; D005233:Fatty Alcohols / Q000737:chemistry*; D017353:Gene Deletion; D005798:Genes, Bacterial; D053520:Marinobacter / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D010641:Phenotype; D012329:RNA, Bacterial / Q000235:genetics; D013379:Substrate Specificity; D015533:Transcriptional Activation; D014885:Waxes / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D004952:Esters; D005233:Fatty Alcohols; D012329:RNA, Bacterial; D014885:Waxes; D000445:Aldehyde Oxidoreductases; D000217:Acyltransferases