title: The emerging links between sirtuins and autophagy.
abstract: Evidence suggests a role for acetylation and deacetylation in regulating autophagy. In this chapter, we describe the methods useful for understanding this important connection. In particular, we discuss methods for the measurements of sirtuin deacetylase activity, in vivo acetylation detection, and the common assays used to monitor both autophagy and the more selective process of mitophagy.
authors.affiliation: Center for Molecular Medicine, NHLBI, National Institutes of Health, Bethesda, MD, USA.
authors.forename: In Hye
authors.initials: IH
authors.lastname: Lee
authors.forename: Jeanho
authors.initials: J
authors.lastname: Yun
authors.forename: Toren
authors.initials: T
authors.lastname: Finkel
mesh_terms: D000107:Acetylation; D000818:Animals; D001343:Autophagy; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D051379:Mice; D018345:Mice, Knockout; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology*; D011499:Protein Processing, Post-Translational; D037761:Sirtuins / Q000502:physiology*
chemical_list: D037761:Sirtuins