title: Helicobacter pylori infection and MDM2 SNP309 association with gastric cancer susceptibility.
abstract: OBJECTIVE
  MDM2 is a negative regulator of p53, and has also been implicated in carcinogenesis. The aim of this study was to define the causal association of Helicobacter pylori infection and the MDM2 SNP309 among northern Iranian patients with gastric cancer (GC). Two hundred and eight patients with GC and 200 cancer-free controls were genotyped for MDM2 SNP309 using the polymerase chain reaction-restriction fragment length polymorphism method. The ureC (glmM) gene was used for detection of H. pylori in this study.
  
  
  RESULTS
  The G allele was found more frequently among patients with GC (55%) than among controls (37%). The risk of GC for MDM2 309G/G genotypes was considerably increased when compared with TT genotypes (odds ratio [OR]=15.93, 95% confidence interval [CI]=4.17-60.84). Among H. pylori-infected subjects, a significantly increased risk of GC associated with the GG genotype was quite clear (OR=14.66, 95% CI=3.54-60).
  
  
  CONCLUSIONS
  The GG genotype was associated with an increased risk of gastric carcinoma. We also found that there is a joint effect of MDM2 SNP309G/G genotype and H. pylori infection for the development of gastric carcinoma. However, the findings need to be verified in large population-based prospective studies for more rigorous analyses of subgroups and gene-environment and gene-gene interactions.
authors.affiliation: 1 Department of Biology, Faculty of Sciences, University of Guilan , Rasht, Iran .
authors.forename: Mohammad Taher
authors.initials: MT
authors.lastname: Moradi
authors.forename: Zivar
authors.initials: Z
authors.lastname: Salehi
authors.forename: Saba Fakhrieh
authors.initials: SF
authors.lastname: Asl
authors.forename: Keyvan
authors.initials: K
authors.lastname: Aminian
authors.forename: Anna Rafiei
authors.initials: AR
authors.lastname: Hashtchin
mesh_terms: D000368:Aged; D002277:Carcinoma / Q000453:epidemiology / Q000235:genetics* / Q000382:microbiology*; D016022:Case-Control Studies; D004198:Disease Susceptibility; D005260:Female; D005838:Genotype; D016481:Helicobacter Infections / Q000150:complications* / Q000453:epidemiology / Q000235:genetics; D016480:Helicobacter pylori; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D051736:Proto-Oncogene Proteins c-mdm2 / Q000235:genetics*; D013274:Stomach Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000382:microbiology*
chemical_list: C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2