title: Structure and interaction with phospholipids of a prokaryotic lipoxygenase from Pseudomonas aeruginosa.
abstract: Lipoxygenases (LOXs), which are essential in eukaryotes, have no confirmed function in prokaryotes that are devoid of polyunsaturated fatty acids. The structure of a secretable LOX from Pseudomonas aeruginosa (Pa_LOX), the first available from a prokaryote, presents significant differences with respect to eukaryotic LOXs, including a cluster of helices acting as a lid to the active center. The mobility of the lid and the structural variability of the N-terminal region of Pa_LOX was confirmed by comparing 2 crystal forms. The binding pocket contains a phosphatidylethanolamine phospholipid with branches of 18 (sn-1) and 14/16 (sn-2) carbon atoms in length. Carbon atoms from the sn-1 chain approach the catalytic iron in a manner that sheds light on how the enzymatic reaction might proceed. The findings in these studies suggest that Pa_LOX has the capacity to extract and modify unsaturated phospholipids from eukaryotic membranes, allowing this LOX to play a role in the interaction of P. aeruginosa with host cells.
authors.affiliation: 1Institut de Biologia Molecular, Parc Científic de Barcelona, Baldiri Reixac 10, 08028 Barcelona, Spain. xcvcri@ibmb.csic.es.
authors.forename: Albert
authors.initials: A
authors.lastname: Garreta
authors.forename: Silvana P
authors.initials: SP
authors.lastname: Val-Moraes
authors.forename: Queralt
authors.initials: Q
authors.lastname: García-Fernández
authors.forename: Montserrat
authors.initials: M
authors.lastname: Busquets
authors.forename: Carlos
authors.initials: C
authors.lastname: Juan
authors.forename: Antonio
authors.initials: A
authors.lastname: Oliver
authors.forename: Antonio
authors.initials: A
authors.lastname: Ortiz
authors.forename: Betty J
authors.initials: BJ
authors.lastname: Gaffney
authors.forename: Ignacio
authors.initials: I
authors.lastname: Fita
authors.forename: Àngels
authors.initials: À
authors.lastname: Manresa
authors.forename: Xavi
authors.initials: X
authors.lastname: Carpena
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D020134:Catalytic Domain; D008084:Lipoxygenase / Q000737:chemistry* / Q000378:metabolism; D062105:Molecular Docking Simulation; D008969:Molecular Sequence Data; D010714:Phosphatidylethanolamines / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011550:Pseudomonas aeruginosa / Q000201:enzymology*
chemical_list: D010714:Phosphatidylethanolamines; C483858:phosphatidylethanolamine; D008084:Lipoxygenase
keywords: enzyme mechanisms; host–pathogen interactions; lipid peroxidation; membrane interaction; protein–phospholipid complexes