title: The cytogenetic effects of acrylamide on Carassius auratus periperial blood cells.
abstract: The cytogenetic effects of acrylamide on golden fish Carassius auratus peripheral blood cells were investigated in vivo. The fish were exposed to 5, 10, and 20 mg/L acrylamide in water for 96 h. Following exposure, peripheral blood samples were obtained to assay for nuclear anomalies and DNA damage. Acrylamide induced micronuclei and other nuclear anomalies including binuclei, nucleoplasm-bridged nuclei, and nuclear buds. The frequencies of these nuclear anomalies were significantly higher after treatment with 10 and 20 mg/L acrylamide (p<0.05). DNA damage was assayed using the alkaline comet assay. Both olive tail moment and the percentage of tail DNA intensity significantly increased after treatment with 20 mg/L acrylamide (p<0.05). In conclusion, acrylamide induces obvious genotoxicity in C. auratus peripheral blood cells.
authors.affiliation: College of Food, Shenyang Agricultural University, 110866 Shenyang City, China. Electronic address: tandehongsy@126.com.
authors.forename: Dehong
authors.initials: D
authors.lastname: Tan
authors.forename: Lingnan
authors.initials: L
authors.lastname: Li
authors.forename: Sha
authors.initials: S
authors.lastname: Wang
authors.forename: Baodong
authors.initials: B
authors.lastname: Wei
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
authors.forename: Bingxin
authors.initials: B
authors.lastname: Sun
authors.forename: Shujuan
authors.initials: S
authors.lastname: Ji
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000818:Animals; D001773:Blood Cells / Q000187:drug effects*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006054:Goldfish; D015162:Micronucleus Tests; D009152:Mutagenicity Tests / Q000379:methods; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D020106:Acrylamide
keywords: Acrylamide; Alkaline comet; BN; C. auratus; CCD; Carassius auratus; DMSO; EDTA; GSH; IARC; International Agency for Research on Cancer; LMP; MN; Micronuclei; NBUD; NPB; OTM; Olive tail moment; PBS; Phosphate buffer saline; Tris; binuclei; charge-coupled device; dimethylsulfoxide; ethylene diamine tetra acetic acid; glutathione; i.p; intraperitoneal; low melting point; micronucleus; nuclear buds; nucleoplasm bridged nuclei; tris (hydroxymethyl) amino methane