title: Modifying the vicinity of the isopeptide bond to reveal differential behavior of ubiquitin chains with interacting proteins: organic chemistry applied to synthetic proteins.
abstract: In every direction: Chemical protein synthesis allows the construction of 14 di-ubiquitin analogues modified in the vicinity of the isopeptide bond to examine their behavior with deubiquitinases and ubiquitin binding domains. The results set the ground for the generation of unique probes for studying the interactions of these chains with various ubiquitin-interacting proteins.
authors.affiliation: Department of Chemistry, Ben Gurion University of the Negev, Beer Sheva 84105 (Israel) http://www.bgu.ac.il/∼abrik.
authors.forename: Najat
authors.initials: N
authors.lastname: Haj-Yahya
authors.forename: Mahmood
authors.initials: M
authors.lastname: Haj-Yahya
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Castañeda
authors.forename: Liat
authors.initials: L
authors.lastname: Spasser
authors.forename: Hosahalli P
authors.initials: HP
authors.lastname: Hemantha
authors.forename: Muhammad
authors.initials: M
authors.lastname: Jbara
authors.forename: Marlin
authors.initials: M
authors.lastname: Penner
authors.forename: Aaron
authors.initials: A
authors.lastname: Ciechanover
authors.forename: David
authors.initials: D
authors.lastname: Fushman
authors.forename: Ashraf
authors.initials: A
authors.lastname: Brik
mesh_terms: D002625:Chemistry, Organic; D008958:Models, Molecular; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D025801:Ubiquitin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D010455:Peptides; D025801:Ubiquitin
keywords: deubiquitinases; peptide ligation; protein synthesis; ubiquitin chains; ubiquitination