title: Synthetic analogues of bovine bactenecin dodecapeptide reduce herpes simplex virus type 2 infectivity in mice.
abstract: We have evaluated the potential of four synthetic peptides (denoted HH-2, 1002, 1006, 1018) with a distant relationship to the host defense peptide bovine bactenecin dodecapeptide for their ability to prevent genital infections with herpes simplex virus type 2 (HSV-2) in mice. All four peptides showed antiviral properties in vitro and reduced HSV-2 infection of Vero cells in a dose-dependent manner. Detailed analysis showed that the peptides were able to interfere with both viral attachment and entry, but not with replication post-entry, and were effective antivirals also when HSV-2 was introduced in human semen. Two of the peptides proved especially effective in reducing HSV-2 infection also in vivo. When admixed with virus prior to inoculation, both HH-2 and 1018 reduced viral replication and disease development in a genital model of HSV-2 infection in mice, and also when using very high infectious doses of HSV-2. These data show that peptides HH-2 and 1018 have antiviral properties and can be used to prevent genital herpes infection in mice.
authors.affiliation: Department of Rheumatology and Inflammation Research, University of Gothenburg, Sweden.
authors.forename: Andrey
authors.initials: A
authors.lastname: Shestakov
authors.forename: Håvard
authors.initials: H
authors.lastname: Jenssen
authors.forename: Robert E W
authors.initials: RE
authors.lastname: Hancock
authors.forename: Inger
authors.initials: I
authors.lastname: Nordström
authors.forename: Kristina
authors.initials: K
authors.lastname: Eriksson
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use*; D018890:Chemoprevention / Q000379:methods; D002522:Chlorocebus aethiops; D004195:Disease Models, Animal; D006558:Herpes Genitalis / Q000517:prevention & control*; D018258:Herpesvirus 2, Human / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008826:Microbial Sensitivity Tests; D010456:Peptides, Cyclic / Q000494:pharmacology / Q000627:therapeutic use*; D016896:Treatment Outcome; D014709:Vero Cells; D053586:Virus Internalization / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D010456:Peptides, Cyclic; C056454:bactenecin
keywords: AMPs; Antimicrobial peptides; Bovine bactenecin; GAG; Genital herpes; HDPs; HNP; HS; HSV-2; Synthetic analogues; anti-microbial peptides; glycosaminoglycans; heparan sulfate; herpes simplex virus type 2; host defense peptides; human neutrophil peptides