title: Protective effects of the total saponins from Rosa laevigata Michx fruit against carbon tetrachloride-induced acute liver injury in mice.
abstract: Saponins are the major chemicals from Rosa laevigata Michx fruit. However, there have no papers to report the activities of the natural product against liver damage. In the present work, total saponins from R. laevigata Michx fruit (RLTS) with purity>70% was produced and then the protective effects of it against CCl4-induced acute liver injury in mice was tested. The results showed that RLTS decreased serum ALT and AST activities compared with model group, as well as the relative liver weight and histological findings. In addition, RLTS remarkably increased the levels of SOD, CAT, GSH-Px, GSH and decreased MDA, iNOS and NO levels in liver. Transmission electron microscopy and TUNEL assays showed that RLTS repaired fragmented DNA and mitochondrial change caused by CCl4. Further investigation demonstrated that RLTS pretreatment down-regulated the protein expression of CYP2E1, ATF6, GRP78, EIF2, COX-2, NF-κB, p53, Caspase-3, Caspase-9, Cytokeratin 18 and the levels of MAPKs phosphorylation, up-regulated Bcl-2 expression, and markedly decreased the gene expression of TNF-α, IL-6, Fas/FasL and Bax. This is the first time to reveal the hepatoprotective effect of total saponins from R. laevigata Michx fruit, which should be developed as a new drug for treatment of live injury in future.
authors.affiliation: College of Pharmacy, Dalian Medical University, 9 Western Lvshun South Road, Dalian 116044, China; Research Institute of Integrated Traditional and Western Medicine of Dalian Medical University, Dalian 116011, China; The First Affiliated Hospital of Dalian Medical University, Dalian 116011, China.
authors.forename: Deshi
authors.initials: D
authors.lastname: Dong
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhang
authors.forename: Lianhong
authors.initials: L
authors.lastname: Yin
authors.forename: Xinqiang
authors.initials: X
authors.lastname: Tang
authors.forename: Youwei
authors.initials: Y
authors.lastname: Xu
authors.forename: Xu
authors.initials: X
authors.lastname: Han
authors.forename: Yan
authors.initials: Y
authors.lastname: Qi
authors.forename: Jinyong
authors.initials: J
authors.lastname: Peng
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000517:prevention & control*; D002853:Chromatography, Liquid / Q000379:methods; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D046529:Microscopy, Electron, Transmission; D020011:Protective Agents / Q000494:pharmacology*; D027826:Rosa / Q000737:chemistry*; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; C000717248:Hspa5 protein, mouse; D020011:Protective Agents; D012503:Saponins; D002251:Carbon Tetrachloride; D019392:Cytochrome P-450 CYP2E1; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: Acute liver injury; Carbon tetrachloride; Hepatoprotective effect; Rosa laevigata Michx; Total saponins