title: Bioguided isolation of myricetin-3-O-β-galactopyranoside with antinociceptive activity from the aerial part of Davilla elliptica St.-Hil.
abstract: BACKGROUND
  Davilla elliptica St.-Hil. (Dilleniaceae) is a medicinal plant traditionally used in Brazil to treat inflammatory processes, to relieve pain, as diuretic, gastro- and hepatoprotective agents.
  
  
  OBJECTIVE
  To undertake the fractionation of the ethanolic extract from Davilla elliptica leaves guided by an antinociceptive assay.
  
  
  METHODS
  The antinociceptive activity was evaluated through the formalin test in mice. Extract fractionation was performed by percolation through silica gel and partition between immiscible solvents, followed by successive column chromatography over Sephadex LH-20 and preparative RP-HPLC. Structure elucidation of the isolated compound was accomplished by spectroscopic data.
  
  
  RESULTS
  The EtOAc and MeOH fractions derived from the crude extract reduced significantly the licking time in the late phase of the formalin test. The bioguided fractionation of the MeOH fraction resulted in the isolation of myricetin-3-O-β-galactopyranoside, which produced significant inhibition on nociception induced by formalin (ID50=0.26 mg/kg; p.o.).
  
  
  CONCLUSIONS
  These results point out that myricetin-3-O-β-galactopyranoside contributes for the antinociceptive effect of Davilla elliptica extract, a constituent considerably more potent than diclofenac, employed as reference drug.
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Campus Pampulha, Belo Horizonte, CEP 31.270-901, Brazil.
authors.forename: Jussara Júlia
authors.initials: JJ
authors.lastname: Campos
authors.forename: Adolfo de Oliveira
authors.initials: Ade O
authors.lastname: Azevedo
authors.forename: José Dias de Souza
authors.initials: JD
authors.lastname: Filho
authors.forename: Andrea Castro
authors.initials: AC
authors.lastname: Perez
authors.forename: Fernão Castro
authors.initials: FC
authors.lastname: Braga
mesh_terms: D000700:Analgesics / Q000302:isolation & purification / Q000627:therapeutic use*; D000818:Animals; D045466:Dilleniaceae; D005419:Flavonoids / Q000302:isolation & purification / Q000627:therapeutic use*; D005557:Formaldehyde; D005697:Galactosides / Q000302:isolation & purification / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy*; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D018515:Plant Leaves
chemical_list: D000700:Analgesics; D005419:Flavonoids; D005697:Galactosides; D010936:Plant Extracts; C588401:myricetin-3-O-galactopyranoside; D005557:Formaldehyde
keywords: Antinociceptive action; Davilla elliptica; Dilleniaceae; Myricetin-3-O-β-galactopyranoside