title: Megaprimer method for mutagenesis of DNA.
abstract: To create mutations in a gene of interest, including point mutations, short insertions, or deletions.
authors.affiliation: Department of Molecular and Cellular Biochemistry and Center for Structural Biology, University of Kentucky, Lexington, KY, USA. Electronic address: craig.vanderkooi@uky.edu.
authors.forename: Craig W
authors.initials: CW
authors.lastname: Vander Kooi
mesh_terms: D004247:DNA / Q000235:genetics*; D017931:DNA Primers; D054643:INDEL Mutation / Q000235:genetics*; D016297:Mutagenesis, Site-Directed / Q000379:methods; D017354:Point Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004247:DNA
keywords: DNA mutagenesis; Design mutagenic primer; Gel purification; Megaprimer method; Polymerase chain reaction (PCR)