title: Cryopreservation of nematode Caenorhabditis elegans in the adult stage.
abstract: Cryopreservation of nematode Caenorhabditis elegans in the adult stage is of importance as the nematode is a powerful research model organism. In this study, we applied the protocol previously established for cryopreservation of the L4 nematode to the adult one, and achieved a survival rate of 84%. When ice seeding was induced with bacteria P. syringae directly added to the nematode suspension instead of using a pre-cooled steel sticking needle, comparable survival rate was obtained after thawing. Moreover, a simple freezing device composed of a polystyrene foam box surrounded by a Dewar vessel put in a deep freezer was developed for a practical use. This simple method obtained a survival rate of 69 ± 4% for the adult nematode after thawing.
authors.affiliation: School of Science and Engineering, Tokyo Denki University, Hatoyama, Saitama, Japan. masakazuhayashii@gmail.com
authors.forename: M
authors.initials: M
authors.lastname: Hayashi
authors.forename: H
authors.initials: H
authors.lastname: Amino
authors.forename: K
authors.initials: K
authors.lastname: Kita
authors.forename: N
authors.initials: N
authors.lastname: Murase
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000254:growth & development / Q000502:physiology* / Q000648:ultrastructure; D015925:Cryopreservation / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D005615:Freezing; D007053:Ice / Q000032:analysis; D044224:Pseudomonas syringae / Q000737:chemistry
chemical_list: D007053:Ice