title: The anti-inflammatory property of human bone marrow-derived mesenchymal stem/stromal cells is preserved in late-passage cultures.
abstract: Human mesenchymal stem/stromal cells (hMSCs) have been reported to improve neural damage via anti-inflammation and multi-differentiation abilities. Here, we investigated immunosuppression effects of hMSCs by mixed-culturing with interferon-γ (IFNγ) stimulated BV-2 mouse microglial cells. We show that hMSCs decreased nitrite oxide (NO) production from BV-2 cells in cell density dependent manner. Aged hMSCs and peroxisome proliferator-activated receptor-γ (PPARγ) knockdown hMSCs decreased differentiation abilities but maintained NO suppressive function. We finally confirmed NO suppression activities of hMSCs in IFNγ-stimulated primary microglia/macrophages. It suggested that hMSCs significantly modified NO production in activated phagocytes and it might be preserved in late passage cultures.
authors.affiliation: Department of Anatomy, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Dandan
authors.initials: D
authors.lastname: Song
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Ohtaki
authors.forename: Tomomi
authors.initials: T
authors.lastname: Tsumuraya
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Junko
authors.initials: J
authors.lastname: Shibato
authors.forename: Randeep
authors.initials: R
authors.lastname: Rakwal
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Xu
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Hiraizumi
authors.forename: Tomio
authors.initials: T
authors.lastname: Inoue
authors.forename: Seiji
authors.initials: S
authors.lastname: Shioda
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology* / Q000473:pathology; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D018920:Coculture Techniques; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000473:pathology / Q000517:prevention & control; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors* / Q000502:physiology; D059630:Mesenchymal Stem Cells / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000276:immunology / Q000473:pathology; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000276:immunology
chemical_list: D018836:Inflammation Mediators; D009569:Nitric Oxide
keywords: Human mesenchymal stem/stromal cells; Inflammation; Interferon-γ; Microglial cells; Nitric oxide