title: IL-17C expression in nasal epithelial cells of chronic rhinosinusitis with nasal polyposis.
abstract: Interleukin 17C (IL-17C) is a functionally distinct member of the IL-17 family that is selectively induced in epithelia by bacterial challenge and inflammatory stimuli. The goal of this study was to explore the expression of IL-17C in nasal epithelial cells and their role in the pathogenesis of chronic rhinosinusitis with nasal polyposis (CRSwNPs). IL-17C expression was detected using immunohistochemistry (IHC) of the epithelial cell layers and using the western blot assay on whole tissue homogenates from control subjects (n = 10) and CRSwNP patients [10 non-eosinophilic polyps and 10 eosinophilic polyps (EPs)]. Expression of IL-17C and P47-phox were evaluated in the human nasal epithelial cells (RPMI-2650 cells) after treatment with staphylococcal enterotoxin B (SEB) and pretreatment with reactive oxygen species (ROS) scavenger, N-acetyl L-cysteine (NAC). Finally, IL-17C expression was demonstrated in eosinophilic rhinosinusitis murine model using IHC. Epithelial expression of IL-17C was higher in nasal polyps (especially in EPs) compared to control mucosa. SEB increased the expression of IL-17C and P47-phox in RPMI-2650 cells. SEB-induced expressions of both IL-17C and P47-phox were significantly decreased in NAC-pretreated cells. Epithelial expression of IL-17C was significantly higher in experimental mice compared to control mice. SEB-induced IL-17C expression in nasal epithelial cells is mediated by ROS production. This pathway may be associated with the pathogenesis of CRSwNP, especially eosinophilic nasal polyps.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Research Institute for Medical Science, Chungnam National University School of Medicine, 282 Munhwa-ro Jung-gu, Daejeon, 301-721, Korea.
authors.forename: Jun
authors.initials: J
authors.lastname: Jin
authors.forename: Ki-Sang
authors.initials: KS
authors.lastname: Rha
authors.forename: Dong Woon
authors.initials: DW
authors.lastname: Kim
authors.forename: Yong Min
authors.initials: YM
authors.lastname: Kim
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002908:Chronic Disease; D004195:Disease Models, Animal; D004802:Eosinophilia / Q000276:immunology; D004847:Epithelial Cells / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology; D007124:Immunoenzyme Techniques; D020381:Interleukin-17 / Q000032:analysis*; D051379:Mice; D019255:NADPH Oxidases / Q000032:analysis; D009297:Nasal Mucosa / Q000276:immunology*; D009298:Nasal Polyps / Q000276:immunology*; D012220:Rhinitis / Q000276:immunology*; D012852:Sinusitis / Q000276:immunology*
chemical_list: C515930:Il17c protein, mouse; D020381:Interleukin-17; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1