title: [Optimization of processing parameters of microwave-assisted extraction technique of Moerella iridescens polysaccharide by response surface methodology].
abstract: OBJECTIVE
  To optimize the extraction conditions of polysaccharide from Moerella iridescens with the microwave-assisted extraction technology by Response surface methodology (RSM).
  
  
  METHODS
  Extracting temperature, extracting time and the solvent/solid ratio were selected as factors. The yield of polysaccharide was used as the responsive value. Based on the single factors experiments, a three-factor-three-level experiments design was developed by Box-Benhken central composite design method. The response surface method was employed to analyze the results of experiments.
  
  
  RESULTS
  The optimum extraction conditions of polysaccharide were as follows: extracting temperature was 45 degrees C, extracting time was 2.7 h and solvent/solid ratio was 50:1. The predictive maximum yield of polysaccharide was 1.17%. The average yield of polysaccharide in 3 validation experiments was 1.19% and the relative error was 1.70%.
  
  
  CONCLUSIONS
  RSM analysis optimizing the extraction conditions for polysaccharide from Moerella iridescens is feasible, which can improve the extraction rate of Moerella iridescens polysaccharide.
authors.affiliation: Sanatorium of Nanjing General Hospital of Nanjing Military Command/Medical Rehabilitation Center of PLA, Nanjing 211131, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiang
authors.forename: Xin
authors.initials: X
authors.lastname: Din
authors.forename: Xue-Lei
authors.initials: XL
authors.lastname: Zhang
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wei
authors.forename: Song-Hua
authors.initials: SH
authors.lastname: Chen
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D008872:Microwaves; D015233:Models, Statistical; D011134:Polysaccharides / Q000302:isolation & purification*; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D011134:Polysaccharides; D014867:Water