title: The effect of bioengineered acellular collagen patch on cardiac remodeling and ventricular function post myocardial infarction.
abstract: Regeneration of the damaged myocardium is one of the most challenging fronts in the field of tissue engineering due to the limited capacity of adult heart tissue to heal and to the mechanical and structural constraints of the cardiac tissue. In this study we demonstrate that an engineered acellular scaffold comprising type I collagen, endowed with specific physiomechanical properties, improves cardiac function when used as a cardiac patch following myocardial infarction. Patches were grafted onto the infarcted myocardium in adult murine hearts immediately after ligation of left anterior descending artery and the physiological outcomes were monitored by echocardiography, and by hemodynamic and histological analyses four weeks post infarction. In comparison to infarcted hearts with no treatment, hearts bearing patches preserved contractility and significantly protected the cardiac tissue from injury at the anatomical and functional levels. This improvement was accompanied by attenuated left ventricular remodeling, diminished fibrosis, and formation of a network of interconnected blood vessels within the infarct. Histological and immunostaining confirmed integration of the patch with native cardiac cells including fibroblasts, smooth muscle cells, epicardial cells, and immature cardiomyocytes. In summary, an acellular biomaterial with specific biomechanical properties promotes the endogenous capacity of the infarcted myocardium to attenuate remodeling and improve heart function following myocardial infarction.
authors.affiliation: Stanford University, Department of Pediatrics, 300 Pasteur Dr., Stanford, CA 94305, USA.
authors.forename: Vahid
authors.initials: V
authors.lastname: Serpooshan
authors.forename: Mingming
authors.initials: M
authors.lastname: Zhao
authors.forename: Scott A
authors.initials: SA
authors.lastname: Metzler
authors.forename: Ke
authors.initials: K
authors.lastname: Wei
authors.forename: Parisha B
authors.initials: PB
authors.lastname: Shah
authors.forename: Andrew
authors.initials: A
authors.lastname: Wang
authors.forename: Morteza
authors.initials: M
authors.lastname: Mahmoudi
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Malkovskiy
authors.forename: Jayakumar
authors.initials: J
authors.lastname: Rajadas
authors.forename: Manish J
authors.initials: MJ
authors.lastname: Butte
authors.forename: Daniel
authors.initials: D
authors.lastname: Bernstein
authors.forename: Pilar
authors.initials: P
authors.lastname: Ruiz-Lozano
mesh_terms: D000818:Animals; D057005:Bioengineering; D001696:Biomechanical Phenomena; D003094:Collagen / Q000494:pharmacology*; D005782:Gels / Q000494:pharmacology; D006321:Heart / Q000187:drug effects* / Q000503:physiopathology*; D006334:Heart Function Tests; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D009203:Myocardial Infarction / Q000503:physiopathology*; D009206:Myocardium / Q000473:pathology; D010969:Plastics / Q000494:pharmacology; D051381:Rats; D016276:Ventricular Function / Q000187:drug effects*; D020257:Ventricular Remodeling / Q000187:drug effects*
chemical_list: D005782:Gels; D010969:Plastics; D003094:Collagen
keywords: Angiogenesis; Cardiac tissue engineering; Cardiomyocyte; Collagen; Heart; Scaffold