title: Pressure-driven microfluidic perfusion culture device for integrated dose-response assays.
abstract: Cell-based assays are widely used in the various stages of drug discovery. Advances in microfluidic systems over the past two decades have enabled them to become a powerful tool for cell-based assays to achieve both reliability and high throughput. The interface between the micro-world and macro-world is important in industrial assay processes. Therefore, microfluidic cell-based assays using pressure-driven liquid handling are an ideal platform for integrated assays. The aim of this article is to review recent advancements in microfluidic cell-based assays focusing on a pressure-driven perfusion culture device. Here, we review the development of microfluidic cell-based assay devices and discuss the techniques involved in designing a microfluidic network, device fabrication, liquid and cell manipulation, and detection schemes for pressure-driven perfusion culture devices. Finally, we describe recent progress in semiautomatic and reliable pressure-driven microfluidic cell-based assays.
authors.affiliation: 1Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Hattori
authors.forename: Shinji
authors.initials: S
authors.lastname: Sugiura
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kanamori
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D004305:Dose-Response Relationship, Drug; D055808:Drug Discovery / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D006874:Hydrostatic Pressure; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods*; D010477:Perfusion
keywords: cell-based assay; microfluidics; perfusion culture