title: Transcriptome sequencing and phylogenetic analysis of floral and leaf MIKC(C) MADS-box and R2R3 MYB transcription factors from the monocot Iris fulva.
abstract: The Louisiana Irises serve as an important system for the study of the evolutionary processes of speciation, including reproductive isolation, hybridization, and adaptation. Sequencing methods today allow for the generation of resources key to elucidating the genetic basis of these phenomena. Here we describe the transcriptomes of floral and young leaf tissue from Iris fulva generated by massively parallel pyrosequencing. In order to identify potential candidates for the study of reproductive isolation and adaptation in the Louisiana Irises we phylogenetically analyzed the type II MIKC(C) MADS-box and R2R3 MYB transcription factors expressed in these tissues. A total of 25 Iris MIKC(C) MADS-box genes in 9 clades and 42 Iris R2R3 MYB genes in 19 clades were identified. Through the identification of eudicot and monocot specific clades, these analyses contribute to our understanding of the evolution of these prominent transcription factor families in the angiosperms.
authors.affiliation: Dept. of Genetics, University of Georgia, 120 E. Green St., Athens, GA 30602, USA. Electronic address: evangeline.ballerini@lifesci.ucsb.edu.
authors.forename: Evangeline S
authors.initials: ES
authors.lastname: Ballerini
authors.forename: Keithanne
authors.initials: K
authors.lastname: Mockaitis
authors.forename: Michael L
authors.initials: ML
authors.lastname: Arnold
mesh_terms: D019143:Evolution, Molecular; D035264:Flowers / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D063990:Gene Ontology; D017343:Genes, Plant; D020570:Genes, myb; D031323:Iris Plant / Q000235:genetics*; D026161:MADS Domain Proteins / Q000235:genetics*; D005810:Multigene Family / Q000235:genetics; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics* / Q000378:metabolism; D017423:Sequence Analysis, RNA; D014157:Transcription Factors / Q000235:genetics*; D059467:Transcriptome
chemical_list: D026161:MADS Domain Proteins; D014157:Transcription Factors
keywords: EST; Evolution; GA; GO; Iris; K(S); Ka; MADS box genes; MYB genes; Monocot; NCBI; National Center for Biotechnology Information; TAIR; TIGR; The Arabidopsis Information Resource; The Institute for Genomic Research; Transcriptome; expressed sequence tag; gene ontology; gibberillic acid; non-synonymous substitution rate; synonymous substitution rate