title: Analysis of monosaccharide composition of Cyclocarya paliurus polysaccharide with anion exchange chromatography.
abstract: A simple, rapid and sensitive analytical method for the determination of monosaccharide composition in Cyclocarya paliurus polysaccharide was developed and validated. This method was based on hydrolysis of the polysaccharides followed by high-performance anion-exchange chromatography analysis. The effects of sodium hydroxide concentration and column temperature on retention and separation of the monosaccharide were investigated with Carbo PAC™ PA10 analytical column. The established method was validated and the results showed that this method had good linearity (R(2), 0.9987-0.9999), adequate accuracy (98.53-102.13% recovery), high precision (relative standard deviation <3.8%) and sensitive detection limits (2.57-7.86 nM), with a simple preparation of the samples (no need to derivatize the samples) and short run time (20 min). The results showed that the C. paliurus polysaccharide consisted of rhamnose, arabinose, galactose, glucose, mannose and xylose in the molar ratio of 1.00:1.85:3.26:3.12:0.85:0.29.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. jhxie@ncu.edu.cn
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Xie
authors.forename: Ming-Yue
authors.initials: MY
authors.lastname: Shen
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Nie
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Ming-Yong
authors.initials: MY
authors.lastname: Xie
mesh_terms: D002852:Chromatography, Ion Exchange; D004563:Electrochemistry; D029525:Juglandaceae / Q000737:chemistry*; D057230:Limit of Detection; D009005:Monosaccharides / Q000032:analysis* / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D009005:Monosaccharides; D011134:Polysaccharides
keywords: Cyclocarya paliurus (Batal.) Iljinskaja; High-performance anion-exchange chromatography; Monosaccharide composition; Polysaccharide