title: Preliminary investigation of anticancer activity by determining the DNA binding and antioxidant potency of new ferrocene incorporated N,N',N″-trisubstituted phenylguanidines.
abstract: Six new bioactive ferrocene based phenylguanidines were successively synthesized and characterized by means of various analytical techniques like elemental analysis, FT-IR, multinuclear ((1)H and (13)C) NMR, UV-Vis spectroscopy and cyclic voltammetry. The interaction of compounds with DNA was investigated by spectroscopic and cyclic voltammetric measurements. The interaction was found to be the electrostatic and the binding constants values were impressively larger. Compounds f-1, f-2, f-3 have slight larger binding constant values ranging from 0.8×10(5) to 2.4×10(5) as compared to g-1, g-2 and g-3 ranging from 7.6×10(4) to 1.1×10(5) which is most probably due to the presence of ferrocene at para position where the delocalization of electrons is maximum. Antioxidant activity was determined by UV-Vis spectrophotometer by using DPPH as a free radical. All the compounds exhibit good antioxidant activity and the results so obtained support the structure activity relationship.
authors.affiliation: Department of Chemistry, Gomal University, Dera Ismail Khan 29050, Pakistan.
authors.forename: Rukhsana
authors.initials: R
authors.lastname: Gul
authors.forename: Amin
authors.initials: A
authors.lastname: Badshah
authors.forename: Azim
authors.initials: A
authors.lastname: Khan
authors.forename: Asif
authors.initials: A
authors.lastname: Junaid
authors.forename: Muhammad Khawar
authors.initials: MK
authors.lastname: Rauf
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D004247:DNA / Q000378:metabolism*; D004563:Electrochemistry; D005296:Ferrous Compounds / Q000737:chemistry*; D006146:Guanidines / Q000737:chemistry / Q000494:pharmacology*; D007202:Indicators and Reagents / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D000075163:Metallocenes; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010851:Picrates / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D005296:Ferrous Compounds; D006146:Guanidines; D007202:Indicators and Reagents; D000075163:Metallocenes; D010851:Picrates; C026943:phenylguanidine; D004247:DNA; C004931:1,1-diphenyl-2-picrylhydrazyl; C004998:ferrocene
keywords: Antioxidant activity; DNA binding; Trisubstituted ferrocenyl phenylguanidines