title: Testing Rhodiola sachalinensis saccharide as cryoprotectant for bovine spermatozoa.
abstract: Rhodiola sachalinensis saccharide (RSS) was extracted from the rhizome of Herba Rhodiolae and was expected as a novel cryoprotectant. The aim of this study was to test the effects of RSS on motility of bull sperm and the activities of superoxide dismutase (SOD), lactate dehydrogenase (LDH), and glutamic oxaloacetic transaminase (GOT) in bull sperm during cryopreservation. Rhodiola sachalinensis saccharide was added at the concentrations of 0.02, 0.04, 0.06, 0.08, and 0.10 mg/mL to the extenders, which were used to store bovine semen. It was found that the RSS-added extends resulted in a higher percentage of cryopreserved sperm motility, mitochondrial activity, and membrane and acrosome integrity than those of RSS-free extenders. The SOD, LDH, and GOT activities were all decreased during the process of freezing and thawing. The extenders supplemented with RSS improved the SOD, LDH, and GOT activities after cryopreservation compared with the RSS-free groups. In conclusion, RSS conferred great cryoprotective capacity to the basic extender for bull spermatozoa during the process of freezing-thawing, and the optimal concentration of RSS for the extender was 0.06 mg/mL.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Hualin
authors.initials: H
authors.lastname: Cao
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Xiuzhu
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China; College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, Hebei 066004, China. Electronic address: ysulqw@126.com.
authors.forename: Qingwang
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Shengxian
authors.initials: S
authors.lastname: Zhou
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Xiangbin
authors.initials: X
authors.lastname: Nan
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Hu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Yamei
authors.initials: Y
authors.lastname: He
mesh_terms: D000177:Acrosome / Q000187:drug effects; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002417:Cattle / Q000502:physiology*; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005615:Freezing; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D029771:Rhodiola / Q000737:chemistry*; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D003451:Cryoprotective Agents; D011134:Polysaccharides; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases
keywords: Rhodiola sachalinensis saccharide; bull semen; cryopreservation; superoxide dismutase