title: Pancreatic lipase inhibitory gallotannins from Galla Rhois with inhibitory effects on adipocyte differentiation in 3T3-L1 cells.
abstract: Activity-guided isolation of a methanolic extract of Galla Rhois using pancreatic lipase and 3T3-L1 adipocytes led to the isolation of seven phenolic compounds: protoaphin-fb (1), 2-O-digalloyl-1,3,4,6-tetra-O-galloyl-β-D-glucose (2), 1,2,3,4,6-penta-O-galloyl-β-D-glucose (3), 1,2,4,6-tetra-O-galloyl-β-D-glucose (4), 3-hydroxy-5-methoxy-phenol 1-O-β-D-glucoside (5), methylgallate (6), and gallic acid (7). Their structures were established on the basis of NMR and MS spectroscopic data interpretation. All isolates were evaluated for their inhibitory effects on pancreatic lipase, and compounds 1-5 exhibited potent inhibitory effects on this enzyme, with IC₅₀ values ranging from 30.6 ± 2.4 to 3.5 ± 0.5 mM. In addition, the highly galloylated compound 2 was also found to induce potent inhibition of adipocyte differentiation in 3T3-L1 cells.
authors.affiliation: Daegyeong Institute for Regional Program Evaluation, Gyeongsan 712-210, Korea.
authors.forename: O Jun
authors.initials: OJ
authors.lastname: Kwon
authors.forename: Jong-Sup
authors.initials: JS
authors.lastname: Bae
authors.forename: Ha Yeong
authors.initials: HY
authors.lastname: Lee
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Hwang
authors.forename: Eun-Woo
authors.initials: EW
authors.lastname: Lee
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Ito
authors.forename: Tae Hoon
authors.initials: TH
authors.lastname: Kim
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D047348:Hydrolyzable Tannins / Q000737:chemistry / Q000494:pharmacology*; D055670:Medicine, Korean Traditional; D051379:Mice; D020799:Pancrelipase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010941:Plant Tumors; D029045:Rhus / Q000737:chemistry*
chemical_list: D004791:Enzyme Inhibitors; D047348:Hydrolyzable Tannins; D010936:Plant Extracts; D020799:Pancrelipase