title: Development and characterization of a novel bioactive polymer with antibacterial and lysozyme-like activity.
abstract: The development and characterization of a novel bioactive polymer based on the immobilization of glucose oxidase enzyme (GOx) in a polyvinyl alcohol (PVA) film showing antibacterial activity is presented. The PVA-GOx composite material was extensively characterized by UV-vis, X-ray Photoelectron (XPS) spectroscopy and by Fourier Transform Infrared (FTIR) spectroscopy to verify the preservation of enzyme structural integrity and activity. The antimicrobial activity of this composite material against Escherichia coli and Vibrio alginolyticus was assessed. Furthermore the lysozyme-like activity of PVA-GOx was highlighted by a standard assay on Petri dishes employing Micrococcus lysodeikticus cell walls. The findings from this study have implications for future investigations related to the employment of PVA-GOx system as a composite material of pharmaceutical and technological interest.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Via Monteroni, 73100, Lecce, Italy.
authors.forename: Maria Rachele
authors.initials: MR
authors.lastname: Guascito
authors.forename: Daniela
authors.initials: D
authors.lastname: Chirizzi
authors.forename: Cosimino
authors.initials: C
authors.lastname: Malitesta
authors.forename: Livia
authors.initials: L
authors.lastname: Giotta
authors.forename: Disma
authors.initials: D
authors.lastname: Mastrogiacomo
authors.forename: Ludovico
authors.initials: L
authors.lastname: Valli
authors.forename: Loredana
authors.initials: L
authors.lastname: Stabili
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001230:Aspergillus / Q000201:enzymology; D015195:Drug Design; D004926:Escherichia coli / Q000187:drug effects; D005949:Glucose Oxidase / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D008837:Micrococcus / Q000187:drug effects; D009113:Muramidase / Q000378:metabolism*; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000494:pharmacology*; D011142:Polyvinyl Alcohol / Q000494:pharmacology; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D014733:Vibrio / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D011108:Polymers; D011142:Polyvinyl Alcohol; D005949:Glucose Oxidase; D009113:Muramidase
keywords: FTIR; XPS; antibacterial; glucose oxidase; lysozyme-like activity; polyvinyl alcohol