title: Effect of thiamine pyrophosphate on ischemia-reperfusion induced oxidative damage in rat kidney.
abstract: OBJECTIVE
  The biochemical effects of thiamine pyrophosphate on ischemia-reperfusion (IR) induced oxidative damage and DNA mutation in rat kidney tissue were investigated, and compared to thiamine.
  
  
  METHODS
  Rats were divided into four groups: renal ischemia-reperfusion (RIR); thiamine pyrophosphate + RIR (TPRIR); thiamine + RIR (TRIR); and sham group (SG).
  
  
  RESULTS
  The results of biochemical experiments have shown that malondialdehyde (MDA) levels in rat kidney tissue after TRIR and TPRIR treatment were 7.2 ± 0.5 (P > 0.05) and 3.3 ± 0.3 (P < 0.0001) μmol/g protein, respectively. The MDA levels in the SG rat kidney tissue and in RIR group were 3.6 ± 0.2 (P < 0.0001) and 7.6 ± 0.6 μmol/g protein, respectively. Total glutathione (tGSH) levels in TRIR, TPRIR, SG, and RIR animal groups were 2.2 ± 0.3 (P > 0.05), 5.8 ± 0.4 (P < 0.0001), 6.2 ± 0.2 (P < 0.0001), and 1.7 ± 0.2 nmol/g protein, respectively. In the TRIR, TPRIR, SG, and RIR animal groups; 8-hydroxyguanine (8-OHGua)/Gua levels, which indicate mutagenic DNA, were 1.75 ± 0.12 (P > 0.05), 0.93 ± 0.1 (P < 0.0001), 0.85 ± 0.08 (P < 0.0001), and 1.93 ± 0.24 pmol/L, respectively.
  
  
  CONCLUSIONS
  It has been shown that thiamine pyrophosphate prevents increase in mutagenic DNA in IR induced oxidative damage, whereas thiamine does not have this effect.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey.
authors.forename: Durdu
authors.initials: D
authors.lastname: Altuner
authors.forename: Nihal
authors.initials: N
authors.lastname: Cetin
authors.forename: Bahadir
authors.initials: B
authors.lastname: Suleyman
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Aslan
authors.forename: Ahmet
authors.initials: A
authors.lastname: Hacimuftuoglu
authors.forename: Mine
authors.initials: M
authors.lastname: Gulaboglu
authors.forename: Neslihan
authors.initials: N
authors.lastname: Isaoglu
authors.forename: Ismail
authors.initials: I
authors.lastname: Demiryilmaz
authors.forename: Halis
authors.initials: H
authors.lastname: Suleyman
mesh_terms: D000818:Animals; D004249:DNA Damage; D005978:Glutathione / Q000378:metabolism; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D013835:Thiamine Pyrophosphate / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D020011:Protective Agents; D008315:Malondialdehyde; C024829:8-hydroxyguanine; D006147:Guanine; D005978:Glutathione; D013835:Thiamine Pyrophosphate
keywords: DNA mutation; ischemia-reperfusion; oxidative damage; rat; thiamine pyrophosphate