title: Analyzing Myc in cell transformation and evolution.
abstract: The myc oncogene was originally identified as a transduced allele (v-myc) in the genome of a highly oncogenic avian retrovirus. The protein product (Myc) of the cellular c-myc proto-oncogene represents the key component of a transcription factor network controlling the expression of a large fraction of all human genes. Myc regulates fundamental cellular processes like growth, metabolism, proliferation, differentiation, and apoptosis. Mutational deregulation of c-myc leading to increased levels of the Myc protein is a frequent event in the etiology of human cancers. In this chapter, we describe cell systems and experimental strategies to monitor and quantify the oncogenic potential of myc alleles and to isolate and characterize transcriptional targets of Myc that are relevant for the cell transformation process. We also describe experimental procedures to study the evolutionary origin of myc and to analyze structure and function of the ancestral myc proto-oncogenes.
authors.affiliation: Center for Chemistry and Biomedicine, Institute of Biochemistry, University of Innsbruck, Innsbruck, Austria.
authors.forename: Markus
authors.initials: M
authors.lastname: Hartl
authors.forename: Klaus
authors.initials: K
authors.lastname: Bister
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D004247:DNA / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods; D019143:Evolution, Molecular; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D061251:Primary Cell Culture / Q000379:methods; D011485:Protein Binding; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics* / Q000378:metabolism*; D011784:Quail; D012190:Retroviridae / Q000235:genetics; D015533:Transcriptional Activation; D014161:Transduction, Genetic; D014162:Transfection; D014410:Tumor Stem Cell Assay
chemical_list: C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc; D004247:DNA