title: Assessing the response of indigenous loquat cultivar Mardan to phytohormones for in vitro shoot proliferation and rooting.
abstract: In vitro cultures of loquat cultivar Mardan were established using shoot apices after treating with NaOCl (5%, 7%, 10%, 12%, 14% (v/v)) for 12 min and HgCl2 (0.01%, 0.05%, 0.10%, 0.20%, 0.25% (w/v)) for 2 min. A maximum survival rate of 70% was recorded after surface sterilization with 10% NaOCl. Caulogenic response was assessed on Murashige and Skoog (MS) medium fortified with assorted combinations of the cytokinins, benzylaminopurine (BAP), kinetin, and N6-(2-isopentyl)adenine (2iP). Treatment of BAP 1.5 mg/L combined with 2iP 9.0 mg/L and kinetin 1.5 mg/L was found to be optimum for shoot morphogenesis in terms of the number and subsequent growth of shoots, while the highest shoot length was yielded by the combination of BAP 0.5 mg/L, kinetin 0.5 mg/L, and 2iP 3 mg/L. Higher levels of cytokinins induced callogenesis, vitrification and stunted growth to some extent. For rhizogenesis, uniform sized micro-shoots were excised and transferred to half-strength MS medium containing auxins. The best rooting expression was observed with naphthaleneacetic acid (NAA) 1 mg/L combined with indole-3-butyric acid (IBA) 2 mg/L and paclobutrazol (PBZ) 1 mg/L.
authors.affiliation: Plant Tissue Culture Laboratory, Department of Horticulture, Pir Mehr Ali Shah-Arid Agriculture University, Rawalpindi 46300, Pakistan.
authors.forename: Nadeem Akhtar
authors.initials: NA
authors.lastname: Abbasi
authors.forename: Tariq
authors.initials: T
authors.lastname: Pervaiz
authors.forename: Ishfaq Ahmed
authors.initials: IA
authors.lastname: Hafiz
authors.forename: Mehwish
authors.initials: M
authors.lastname: Yaseen
authors.forename: Azhar
authors.initials: A
authors.lastname: Hussain
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D001593:Benzyl Compounds / Q000494:pharmacology; D004202:Disinfectants / Q000494:pharmacology; D031983:Eriobotrya / Q000187:drug effects* / Q000254:growth & development*; D007211:Indoles / Q000494:pharmacology; D007701:Kinetin / Q000494:pharmacology; D008627:Mercuric Chloride / Q000494:pharmacology; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development; D011687:Purines / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology
chemical_list: D001593:Benzyl Compounds; D004202:Disinfectants; D007211:Indoles; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; D014230:Triazoles; C014612:indolebutyric acid; D008627:Mercuric Chloride; C053370:paclobutrazol; D012973:Sodium Hypochlorite; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin