title: Liquid chromatography-mass spectrometry determination in plasma of maslinic acid, a bioactive compound from Olea europaea L.
abstract: Maslinic acid, a pentacyclic triterpene from Olea europaea L., exerts hypoglycemic, antioxidant, cardioprotective and antitumoral activities. Here, an LC-APCI-MS method to determine this compound in plasma is presented as a first step in pharmacokinetic studies. Maslinic acid was extracted from plasma with ethyl acetate and separated on a C18 column using a gradient elution of water and acetonitrile. The target ions were m/z 471.3 for maslinic acid and m/z 455.3 for I.S. The method was validated by the analysis of spiked plasma samples obtaining a linear correlation, a recovery of 99.0±0.9% and a quantification limit of 5 nM. The precision and accuracy were ≤8.38% and ≤4.82%, respectively. Finally, the method was verified by measuring the rat plasmatic concentrations 24h after the single oral administration of 10, 25 and 50 mg/kg of maslinic acid, thereby ensuring that the procedure is appropriate for its use in bioavailability studies.
authors.affiliation: Departament de Fisiologia and Institut de Recerca en Nutrició i Seguretat Alimentària (INSA-UB), Facultat de Farmàcia, Universitat de Barcelona (UB), Av. Joan XXIII s/n, 08028 Barcelona, Spain. marta_sanchez@ub.edu
authors.forename: Marta
authors.initials: M
authors.lastname: Sánchez-González
authors.forename: Glòria
authors.initials: G
authors.lastname: Lozano-Mena
authors.forename: M Emília
authors.initials: ME
authors.lastname: Juan
authors.forename: Andrés
authors.initials: A
authors.lastname: García-Granados
authors.forename: Joana M
authors.initials: JM
authors.lastname: Planas
mesh_terms: D000818:Animals; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D031658:Olea / Q000737:chemistry*; D010936:Plant Extracts / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D014315:Triterpenes / Q000097:blood* / Q000493:pharmacokinetics; D009828:Oleanolic Acid / Q000031:analogs & derivatives
chemical_list: D010936:Plant Extracts; D014315:Triterpenes; C412811:maslinic acid; D009828:Oleanolic Acid
keywords: LC-MS; Maslinic acid; Olives; Pentacyclic triterpenes; Rat plasma