title: Mass spectrometry-based detection of protein acetylation.
abstract: Improved sample preparation techniques and increasingly sensitive mass spectrometry (MS) analysis have revolutionized the study of protein post-translational modifications (PTMs) (Rush et al., Nat Biotechnol 23:94-101, 2005). Here, we describe a general approach for immunopurification and MS-based identification of acetylated proteins in biological samples. This approach is useful to characterize changes in the acetylome in response to biological interventions (Schwer et al., Aging Cell 8:604-606, 2009).
authors.affiliation: Cell Signaling Technology, Danvers, MA, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Jeffrey C
authors.initials: JC
authors.lastname: Silva
authors.forename: Mary E
authors.initials: ME
authors.lastname: Skinner
authors.forename: David B
authors.initials: DB
authors.lastname: Lombard
mesh_terms: D000107:Acetylation; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D015151:Immunoblotting; D047468:Immunoprecipitation / Q000379:methods*; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D011506:Proteins