title: Genetic analysis of an H5N2 highly pathogenic avian influenza virus isolated from a chicken in a live bird market in Northern Vietnam in 2012.
abstract: In August 2012, A/chicken/Vietnam/OIE-2215/2012 (H5N2) was isolated from a chicken in a live bird market (LBM) in Northern Vietnam. Intravenous pathogenicity test revealed that this virus is highly pathogenic in chickens. The PA, HA, NP and M, PB2 and NA, and PB1 and NS genes of the isolate were phylogenetically closely related to those of A/duck/Vietnam/OIE-2202/2012 (H5N1) of clade 2.3.2.1, A/chicken/Vietnam/OIE-1611/2012 (H9N2) and A/chicken/Vietnam/OIE-2468/2012 (H9N2), respectively. All of these viruses were isolated from birds in LBMs in the same province. These results indicate that A/chicken/Vietnam/OIE-2215/2012 (H5N2) is a genetic reassortant and that surveillance of avian influenza in LBMs and stamping out policy are essential for the eradication of highly pathogenic avian influenza viruses from Asia.
authors.affiliation: Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nishi
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Okamatsu
authors.forename: Kenji
authors.initials: K
authors.lastname: Sakurai
authors.forename: Huy Duc
authors.initials: HD
authors.lastname: Chu
authors.forename: Long Pham
authors.initials: LP
authors.lastname: Thanh
authors.forename: Long
authors.initials: L
authors.lastname: van Nguyen
authors.forename: Nam
authors.initials: N
authors.lastname: van Hoang
authors.forename: Diep Nguyen
authors.initials: DN
authors.lastname: Thi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sakoda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kida
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D002645:Chickens; D053125:Influenza A Virus, H5N2 Subtype / Q000235:genetics*; D005585:Influenza in Birds / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D016865:Reassortant Viruses / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014744:Vietnam
chemical_list: D012367:RNA, Viral