title: Blood compatible N-maleyl chitosan-graft-PAMAM copolymer for enhanced gene transfection.
abstract: To improve transfection efficiency, we prepared N-maleyl chitosan-graft-polyamidoamine (NMCTS-graft-PAMAM) copolymer. Self-assembled NMCTS-graft-PAMAM/pDNA complexes were prepared by complex coacervation method at different N/P (nitrogen to phosphate ratio) ratios. The copolymer effectively formed complexes with pDNA at lower N/P ratio (N/P ratio 1.0) than that of unmodified chitosan (N/P ratio 2.0) and the complexes were spherical with particle size of 100-150 nm. The copolymer showed significant protection of DNA from nuclease attack with lower toxicity against HeLa cell. The copolymer also showed no noticeable hemolytic effects up to 10mg/mL indicating no detectable disturbance of the red blood cell membranes. The transfection efficiency of the copolymer was increased significantly compared to that of chitosan and reached up to 36±2% at N/P ratio 7.0 which was higher than that of PEI (30±3% at N/P ratio 10). Therefore, the copolymer may be a strong alternative candidate as effective nonviral vector.
authors.affiliation: Department of Polymer Science & Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 700009, India.
authors.forename: Kishor
authors.initials: K
authors.lastname: Sarkar
authors.forename: Abhisek
authors.initials: A
authors.lastname: Chatterjee
authors.forename: Gopal
authors.initials: G
authors.lastname: Chakraborti
authors.forename: Patit P
authors.initials: PP
authors.lastname: Kundu
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry; D010316:Particle Size; D011073:Polyamines / Q000737:chemistry*; D012995:Solubility; D014162:Transfection / Q000379:methods*; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; C531249:Poly(amidoamine); D011073:Polyamines; D014867:Water; D004247:DNA; D048271:Chitosan; D003850:Deoxyribonuclease I
keywords: Chitosan; Hemolysis; In vitro transfection; Nanoparticle; PAMAM dendrimer; Self-assembled