title: Analysis of DNA by Southern blotting.
abstract: The purpose of this protocol is to detect specific DNA sequences in a complex sample by hybridization to a labeled probe.
authors.affiliation: Ear Institute, University College London, London, United Kingdom. Electronic address: gary638@gmail.com.
authors.forename: Gary
authors.initials: G
authors.lastname: Glenn
authors.forename: Lefkothea-Vasiliki
authors.initials: LV
authors.lastname: Andreou
mesh_terms: D015139:Blotting, Southern / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization / Q000379:methods*
chemical_list: D004247:DNA
keywords: Digoxigenin-11-dUTP; Hybridized probe; Labeled probe; Nylon membrane; Restriction enzyme(s); Southern blotting; [α-(32)P]-dCTP