title: Preparation and characterization of stable cross-linked enzyme aggregates of novel laccase enzyme from Shewanella putrefaciens and using malachite green decolorization.
abstract: A novel type laccase from Shewanella putrefaciens was identified, expressed in Escherichia coli, characterized, prepared in cross-linked enzyme aggregates (CLEA) for industrial applications and investigated of decolorization activity on malchite green dye. Enzyme characterization was investigated by enzyme assay, SDS-PAGE and other biochemical reactions. Moreover, cross-linked enzyme aggregates were prepared and characterized. Saturated ammonium sulphate solution was used as the precipitating agent and cross linked with glutaraldehyde. These CLEA-laccase aggregates showed more catalytic efficiency and more stabilities compared to free laccase against harsh conditions of thermal and chemical agents as well as high reusability. Also it showed more decolorization ability. These results suggest that this CLEA is potentially usable in industrial applications.
authors.affiliation: Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, 34500 Buyukcekmece, Istanbul, Turkey. Electronic address: zeynepaydin@fatih.edu.tr.
authors.forename: Zeynep Aydin
authors.initials: ZA
authors.lastname: Sinirlioglu
authors.affiliation: Department of Chemistry, Faculty of Art and Sciences, Fatih University, 34500 Buyukcekmece, Istanbul, Turkey. Electronic address: dsinirlioglu@fatih.edu.tr.
authors.forename: Deniz
authors.initials: D
authors.lastname: Sinirlioglu
authors.affiliation: Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, 34500 Buyukcekmece, Istanbul, Turkey. Electronic address: fahriakbas@fatih.edu.tr.
authors.forename: Fahri
authors.initials: F
authors.lastname: Akbas
mesh_terms: D002384:Catalysis; D002701:Chloramphenicol / Q000737:chemistry; D003001:Cloning, Molecular; D004396:Coloring Agents / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004800:Enzymes, Immobilized / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D005976:Glutaral / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D042845:Laccase / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry; D012394:Rosaniline Dyes / Q000737:chemistry; D020614:Shewanella putrefaciens / Q000201:enzymology*; D013696:Temperature
chemical_list: D004396:Coloring Agents; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D011994:Recombinant Proteins; D012394:Rosaniline Dyes; C005095:malachite green; D002701:Chloramphenicol; D042845:Laccase; D005976:Glutaral
keywords: Cloning laccase enzyme; Cross-linked enzyme aggregates; Decolorization; Shewanella putrefaciens