title: Simultaneous visualization of multiple protein interactions using multicolor bimolecular fluorescence complementation (BiFC) analysis.
abstract: This multicolor bimolecular fluorescence complementation procedure is designed for the analysis of alternative protein interactions in cultured mammalian cells, but it can be readily adapted to any cell type or aerobically grown organism that can be genetically modified to express the fusion proteins.
authors.forename: Tom K
authors.initials: TK
authors.lastname: Kerppola
mesh_terms: D002478:Cells, Cultured; D003116:Color; D003584:Cytological Techniques / Q000379:methods*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins