title: In vitro comparison of enzymatic effects among Brazilian Bothrops spp. venoms.
abstract: In various types of snake venom, the major toxic components are proteinases and members of the phospholipase A2 family, although other enzymes also contribute to the toxicity. In this study, we evaluated the proteolytic, phospholipase, and L-Amino acid oxidase activities in the venom of five Bothrops species-Bothrops jararaca, Bothrops jararacussu, Bothrops moojeni, Bothrops neuwiedi, and Bothrops alternatus-all of which are used in the production of commercial antivenom, prepared in horses. The enzymatic activities of each species' venom were classified as high, moderate, or low. B. moojeni venom demonstrated the highest enzymatic activity profile, followed by the venom of B. neuwiedi, B. jararacussu, B. jararaca, and B. alternatus. To our knowledge, this is the first study to compare all of these enzymes from multiple species, which is significant in view of the activity of L-amino acid oxidase across Bothrops species.
authors.affiliation: Department of Biochemistry and Immunology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900, 14049-900 Ribeirão Preto, SP, Brazil.
authors.forename: Lucas B
authors.initials: LB
authors.lastname: Campos
authors.forename: Manuela B
authors.initials: MB
authors.lastname: Pucca
authors.forename: Eduardo C
authors.initials: EC
authors.lastname: Roncolato
authors.forename: Thaís B
authors.initials: TB
authors.lastname: Bertolini
authors.forename: Joaquim C
authors.initials: JC
authors.lastname: Netto
authors.forename: José E
authors.initials: JE
authors.lastname: Barbosa
mesh_terms: D000818:Animals; D017837:Bothrops; D001938:Brazil; D002417:Cattle; D003435:Crotalid Venoms / Q000737:chemistry / Q000201:enzymology*; D050830:L-Amino Acid Oxidase / Q000737:chemistry; D010447:Peptide Hydrolases / Q000737:chemistry; D010740:Phospholipases / Q000737:chemistry; D059748:Proteolysis; D012756:Sheep; D013045:Species Specificity
chemical_list: D003435:Crotalid Venoms; D050830:L-Amino Acid Oxidase; D010740:Phospholipases; D010447:Peptide Hydrolases
keywords: Bothrops; Phospholipase A2; Proteinase; Venom; l-amino acid oxidase