title: Regulated capture by exosomes of mRNAs for cytoplasmic tRNA synthetases.
abstract: Although tRNA synthetases are enzymes that catalyze the first step of translation in the cytoplasm, surprising functions unrelated to translation have been reported. These studies, and the demonstration of novel activities of splice variants, suggest a far broader reach of tRNA synthetases into cell biology than previously recognized. Here we show that mRNAs for most tRNA synthetases can be detected in exosomes. Also detected in exosomes was an mRNA encoding a unique splice variant that others had associated with prostate cancer. The exosomal mRNAs encoding the native synthetase and its cancer-associated splice variant could be translated in vitro and in mammalian cells into stable proteins. Other results showed that selection by exosomes of the splice variant mRNA could be regulated by an external stimulus. Thus, a broad and diverse regulated pool of tRNA synthetase-derived mRNAs is packaged for genetic exchange.
authors.affiliation: From the IAS HKUST-Scripps R&D Laboratory, Institute for Advanced Study and.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Xu
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.forename: Wing-Sze
authors.initials: WS
authors.lastname: Lo
authors.forename: Ching-Fun
authors.initials: CF
authors.lastname: Lau
authors.forename: Leslie A
authors.initials: LA
authors.lastname: Nangle
authors.forename: Xiang-Lei
authors.initials: XL
authors.lastname: Yang
authors.forename: Mingjie
authors.initials: M
authors.lastname: Zhang
authors.forename: Paul
authors.initials: P
authors.lastname: Schimmel
mesh_terms: D017398:Alternative Splicing; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D001943:Breast Neoplasms; D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism; D055354:Exosomes / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D019169:Jurkat Cells; D061986:MCF-7 Cells; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D000604:Amino Acyl-tRNA Synthetases; C020659:glutaminyl-tRNA synthetase
keywords: Alternative Splicing; Aminoacyl tRNA Synthetase; Cell Biology; Exosomes; Jurkat Cell Exosome mRNAs; mRNA; tRNA Synthetase Splice Variant