title: Differences in biofilm formation of produce and poultry Salmonella enterica isolates and their persistence on spinach plants.
abstract: Spinach plants were irrigated biweekly with water containing 2.1 log CFU Salmonella/100 ml water (the maximum Escherichia coli MPN recommended by the Leafy Greens Marketing Agreement; LGMA), or 4.1 CFU Salmonella/100 ml water to determine Salmonella persistence on spinach leaves. Green Fluorescent protein expressing Salmonella were undetectable by most-probable number (MPN) at 24 h and 7 days following each irrigation event. This study indicates that Salmonella are unlikely to persist on spinach leaves when irrigation water is contaminated at a level below the LGMA standards. In a parallel study, persistence of Salmonella isolated from poultry or produce was compared following biweekly irrigation of spinach plants with water containing 6 log CFU Salmonella/100 ml. Produce Salmonella isolates formed greater biofilms on polystyrene, polycarbonate and stainless steel surfaces and persisted at significantly higher numbers on spinach leaves than those Salmonella from poultry origin during 35 days study. Poultry Salmonella isolates were undetectable (<1 log CFU/g) on spinach plants 7 days following each irrigation event when assayed by direct plating. This study indicates that Salmonella persistence on spinach leaves is affected by the source of contamination and the biofilm forming ability of the strain.
authors.affiliation: US Department of Agriculture, Agricultural Research Service, Beltsville, MD 20707, USA. jitu.patel@ars.usda.gov
authors.forename: Jitendra
authors.initials: J
authors.lastname: Patel
authors.forename: Manpreet
authors.initials: M
authors.lastname: Singh
authors.forename: Dumitru
authors.initials: D
authors.lastname: Macarisin
authors.forename: Manan
authors.initials: M
authors.lastname: Sharma
authors.forename: Daniel
authors.initials: D
authors.lastname: Shelton
mesh_terms: D057914:Agricultural Irrigation; D000818:Animals; D018441:Biofilms; D003257:Consumer Product Safety; D005506:Food Contamination; D018515:Plant Leaves / Q000254:growth & development / Q000382:microbiology; D011200:Poultry / Q000382:microbiology*; D019779:Salmonella enterica / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D018724:Spinacia oleracea / Q000254:growth & development / Q000382:microbiology*
keywords: Biofilm; Persistence; Salmonella; Spinach