title: Curcumin protects rat heart mitochondria against anoxia-reoxygenation induced oxidative injury.
abstract: It is an important therapeutic strategy to protect mitochondria from oxidative stress, especially during ischemia-reperfusion. Curcumin is a naturally occurring phenolic compound isolated as a yellow pigment from turmeric (Curcuma longa). This compound has received much attention due to its diversity of biological and pharmacological activities. In this study, an attempt has been made to evaluate the protective effects of curcumin on rat heart mitochondrial injuries induced by in vitro anoxia-reoxygenation. It was found that curcumin added before anoxia or immediately prior to reoxygenation exhibited remarkable protective effects against anoxia-reoxygenation induced oxidative damage to mitochondria, in concentrations ranging from picomoles to micromoles, with EC50s in the nanomolar range. The protective effects include inhibition of the decrease of state 3 respiratory activity, the decrease of respiratory control ratio (RCR) and ADP:oxygen (ADP:O) ratio, as well as the increase of state 4 respiratory activity; inhibition of the decrease of the membrane fluidity; inhibition of lipoperoxidation and protein carbonylation; as well as inhibition of the enhanced release of cardiolipin (CL) and cytochrome c (Cyt c). These results demonstrate the superior antioxidative properties of curcumin, and make it a promising candidate for the prevention and (or) therapy for ischemia-reperfusion injuries and the related free radical initiated diseases.
authors.affiliation: Department of Cardiovascular Medicine, First Affiliated Hospital of Lanzhou University, 1 West Dong Gang Road, Lanzhou, Gansu 730000, P.R. China.
authors.forename: Peihan
authors.initials: P
authors.lastname: Xu
authors.forename: Yali
authors.initials: Y
authors.lastname: Yao
authors.forename: Pengjun
authors.initials: P
authors.lastname: Guo
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.forename: Bingwu
authors.initials: B
authors.lastname: Yang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002308:Cardiolipins / Q000378:metabolism; D015687:Cell Hypoxia; D003474:Curcumin / Q000494:pharmacology*; D045304:Cytochromes c / Q000378:metabolism; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D018384:Oxidative Stress / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors
chemical_list: D000975:Antioxidants; D002308:Cardiolipins; D017392:Thiobarbituric Acid Reactive Substances; D000244:Adenosine Diphosphate; D045304:Cytochromes c; D003474:Curcumin