title: Transfer of auxinic herbicide resistance fromBrassica kabertoBrassica junceaandBrassica rapathrough embryo rescue.
abstract: Auxinic herbicides are widely used in agriculture to selectively control broadleaf weeds. Prolonged use of auxinic herbicides has resulted in the evolution of resistance to these herbicides in some biotypes ofBrassica kaber(wild mustard), a common weed in agricultural crops. In this study, auxinic herbicide resistance fromB. kaberwas transferred toBrassica junceaandBrassica rapa, two commercially importantBrassicacrops, by traditional breeding coupled within vitroembryo rescue. A high frequency of embryo regeneration and hybrid plant establishment was achieved. Transfer of auxinic herbicide resistance fromB. kaberto the hybrids was assessed by whole-plant screening of hybrids with dicamba, a widely used auxinic herbicide. Furthermore, the hybrids were tested for fertility (both pollen and pistil) and their ability to produce backcross progeny. The auxinic herbicide-resistant trait was introgressed intoB. junceaby backcross breeding. DNA ploidy of the hybrids as well as of the backcross progeny was estimated by flow cytometry. Creation of auxinic herbicide-resistantBrassicacrops by non-transgenic approaches should facilitate effective weed control, encourage less tillage, provide herbicide rotation options, minimize occurrence of herbicide resistance, and increase acceptance of these crops.
authors.affiliation: Department of Agronomy, Kansas State University, Manhattan, KS USA.
authors.forename: J
authors.initials: J
authors.lastname: Mithila
authors.forename: J Christopher
authors.initials: JC
authors.lastname: Hall
keywords: Auxinic herbicides; Dicamba; Embryo rescue; Introgression