title: [IL-1β promotes the hematopoietic support of human umbilical cord mesenchymal stem cells].
abstract: This study was aimed to investigate the effect of IL-1β on hematopoietic support of human umbilical cord mesenchymal stem cells (hUC-MSC). 2×10(6) hUC-MSC were seeded in 75 cm(2) flasks, after adherence to wall for 2 h, 10 ng/ml IL-1β was added in hUC-MSC supernatant and cultured for 36 h, then the culture supernatants and cells were harvested. The effect of conditioned medium with/without IL-1β on CD34(+) cell hematopoietic support was observed, mRNA expression changes of hUC-MSC cultured in medium with/without IL-1β were monitored by real time PCR, the differences in hematopoiesis-related factors were detected by ELISA. The results showed that the conditioned culture medium of hUC-MSC with IL-1β enhanced the ability to form colony of CD34(+) cells, especially CFU-G and CFU-GM in vitro; IL-1β promoted the mRNA expression of GM-CSF, G-CSF, IL-6 on MSC; IL-1β also promoted the secretion of GM-CSF, G-CSF, and IL-6 protein from hUC-MSC. It is concluded that IL-1β enhances hematopoietic support capacity especially, capability of MSC to myeloid differentiation.
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
authors.forename: Yue-Ru
authors.initials: YR
authors.lastname: Ji
authors.forename: Zhou-Xin
authors.initials: ZX
authors.lastname: Yang
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Li
authors.forename: Zhi-Bo
authors.initials: ZB
authors.lastname: Han
authors.forename: Ying
authors.initials: Y
authors.lastname: Chi
authors.forename: Zhong-Chao
authors.initials: ZC
authors.lastname: Han
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006413:Hematopoietic System / Q000187:drug effects; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology*; D015850:Interleukin-6 / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D003470:Culture Media; C508600:IL6 protein, human; D053583:Interleukin-1beta; D015850:Interleukin-6; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor