title: Composition analysis and anti-proliferation activity of polysaccharides from Dendrobium chrysotoxum.
abstract: Water-soluble polysaccharides (DCCP, DCPP) were extracted from the stems of Dendrobium chrysotoxum using boiling water and ultrasound. DCPP were successively purified by chromatography on DEAE-Cellulose-52 and Sephadex G-200 column, giving three major polysaccharide fractions termed DCPP-I, DCPP-I-a and DCPP-II. Among these fractions, DCPP-I-a exhibited the highest abundance (79.5%). The number-average molecular weight and weight-average molecular weight of DCPP-I-a were 67 kDa and 122 kDa, respectively. Monosaccharide components analysis indicated that DCPP-I-a were composed of xylose, glucose, galactose in a molar ratio of 1.44:6.93:12.79. Infrared spectra (IR) and (1)H NMR showed that DCPP-I-a was composed of a β-D pyran ring with a β-configuration. The evaluation of anti-proliferation activity suggested that DCPP-I-a and DCPP-II had a higher inhibitory effect on the SPC-A-1 cells than DCCP as determined with in vitro anti-proliferation test.
authors.affiliation: College of Life Science, South China Normal University, Guangdong Province Key Lab for Biotechnology of Plant Development, Guangzhou 510631, PR China. Electronic address: 41378579@qq.com.
authors.forename: Yan-Dong
authors.initials: YD
authors.lastname: Sun
authors.forename: Zai-Hua
authors.initials: ZH
authors.lastname: Wang
authors.forename: Qing-Sheng
authors.initials: QS
authors.lastname: Ye
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D031667:Dendrobium / Q000737:chemistry*; D006801:Humans; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D009005:Monosaccharides; D011134:Polysaccharides
keywords: Anti-proliferation activity; Dendrobium chrysotoxum; Polysaccharide