title: [Genetic variability of anaplasmataceae bacteria determined in Haemaphysalis spp. and Dermacentor sp. ticks in the territory of the Russian Far East].
abstract: Totally 484 Haemaphysalis japonica, 359 Haemaphysalis concinna and 221 Dermacentor silvarumn collected in Amur region and Khabarovsk Territory of the Russian Far East were examined on the presence of Anaplasmataceae bacteria using nested PCR. All positive samples were characterized by analysis of the 16S rRNA gene and/or groESL operone nucleotide sequences. Forty nine H. japonica and three H. concinna were shown to contain DNA of two new Ehrlichia genetic variants. These genetic variants on the basis of the 16S rRNA gene and groESL operone nucleotide sequences analysis were most closely related to Ehrlichia spp. revealed in Haemaphysalis spp. ticks in Japan. Four H. concinna from Amur region were shown to contain DNA of a new Anaplasma bovis genetic variant, which corresponded to A. bovis genetic variant revealed in a red gray-backed vole and a Siberian chipmunk from the Far East. Three H. concinna and nine D. silvarum contained DNA of non-typical bacteria which can't be attributed to any Anaplasmataceae genera based on the determined sequences of the 16S rRNA gene fragments. The revealed non-typical bacteria on the basis of 16S rRNA gene sequences significantly differed from each other and didn't form a separate genetic group.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, RAS, Novosibirsk, Russia
authors.forename: V A
authors.initials: VA
authors.lastname: Rar
authors.forename: T I
authors.initials: TI
authors.lastname: Epikhina
authors.forename: N M
authors.initials: NM
authors.lastname: Pukhovskaya
authors.forename: N P
authors.initials: NP
authors.lastname: Vysochina
authors.forename: L I
authors.initials: LI
authors.lastname: Ivanov
mesh_terms: D000710:Anaplasmataceae / Q000235:genetics*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D018833:Chaperonins / Q000235:genetics; D014644:Genetic Variation; D026863:Ixodidae / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012800:Siberia
chemical_list: D001426:Bacterial Proteins; C097970:GroESL protein, Bacteria; D012336:RNA, Ribosomal, 16S; D018833:Chaperonins