title: Explanatory chapter: PCR primer design.
abstract: This chapter is intended as a guide on polymerase chain reaction (PCR) primer design (for information on PCR, see General PCR and Explanatory Chapter: Troubleshooting PCR). In the next section, general guidelines will be provided, followed by a discussion on primer design for specific applications. A list of recommended software tools is shown at the end.
authors.affiliation: Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom. Electronic address: rubenaf.uo@gmail.com.
authors.forename: Rubén
authors.initials: R
authors.lastname: Álvarez-Fernández
mesh_terms: D000465:Algorithms; D017931:DNA Primers; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012984:Software
chemical_list: D017931:DNA Primers
keywords: Homologous recombination; Multiplex PCR; PCR Primer design; Primer length; Real-time PCR; Reverse-transcription PCR (RT-PCR); Target cloning