title: Nuclear pool of phosphatidylinositol 4 phosphate 5 kinase 1α is modified by polySUMO-2 during apoptosis.
abstract: Phosphatidylinositol 4 phosphate 5 kinase 1α (PIP5K) is mainly localized in the cytosol and plasma membrane. Studies have also indicated its prominent association with nuclear speckles. The exact nature of this nuclear pool of PIP5K is not clear. Using biochemical and microscopic techniques, we have demonstrated that the nuclear pool of PIP5K is modified by SUMO-1 in HEK-293 cells stably expressing PIP5K. Moreover, this SUMOylated pool of PIP5K increased during apoptosis. PolySUMO-2 chain conjugated PIP5K was detected by pull-down experiment using affinity-tagged RNF4, a polySUMO-2 binding protein, during late apoptosis.
authors.affiliation: Department of Biochemistry, University of Calcutta, India.
authors.forename: Rajarshi
authors.initials: R
authors.lastname: Chakrabarti
authors.forename: Debajit
authors.initials: D
authors.lastname: Bhowmick
authors.forename: Varsha
authors.initials: V
authors.lastname: Bhargava
authors.forename: Kaushik
authors.initials: K
authors.lastname: Bhar
authors.forename: Anirban
authors.initials: A
authors.lastname: Siddhanta
mesh_terms: D017209:Apoptosis; D002466:Cell Nucleolus / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000032:analysis / Q000378:metabolism*; D025842:SUMO-1 Protein / Q000378:metabolism; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism*
chemical_list: D025842:SUMO-1 Protein; C117909:SUMO2 protein, human; D025841:Small Ubiquitin-Related Modifier Proteins; D017853:Phosphotransferases (Alcohol Group Acceptor); C020077:1-phosphatidylinositol-4-phosphate 5-kinase
keywords: Apoptosis; Phosphatidylinositol 4 phosphate 5 kinase 1α; PolySUMOylation; RNF4; SUMO