title: Fucan effect on CHO cell proliferation and migration.
abstract: Fucan is a term used to denominate sulfated L-fucose rich polysaccharides. Here, a heterofucan, named fucan B, was extracted from the Spatoglossum schröederi seaweed. This 21.5 kDa galactofucan inhibited CHO-K1 proliferation and migration when fibronectin was the substrate. Fucan B derivatives revealed that such effects depend on their degree of sulfation. Fucan B did not induce cell death, but promoted G1 cell cycle arrest. Western blotting and flow cytometry analysis suggest that fucan B binds to fibronectin and activates integrin, mainly integrin α5β1, which induces FAK/RAS/MEK/ERK activation. FAK activation inhibits CHO-K1 migration on fibronectin and ERK blocks cell cycle progression. This study indicates that fucan B could be applied in developing new antitumor drugs.
authors.affiliation: Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte - UFRN, Av. Senador Salgado Filho, 3000, Lagoa Nova, 59072-970 Natal, RN, Brazil.
authors.forename: Leonardo Thiago Duarte Barreto
authors.initials: LT
authors.lastname: Nobre
authors.forename: Arthur Anthunes Jacome
authors.initials: AA
authors.lastname: Vidal
authors.forename: Jailma
authors.initials: J
authors.lastname: Almeida-Lima
authors.forename: Ruth Medeiros
authors.initials: RM
authors.lastname: Oliveira
authors.forename: Edgar Jean
authors.initials: EJ
authors.lastname: Paredes-Gamero
authors.forename: Valquiria Pereira
authors.initials: VP
authors.lastname: Medeiros
authors.forename: Edvaldo Silva
authors.initials: ES
authors.lastname: Trindade
authors.forename: Celia Regina Cavichiolo
authors.initials: CR
authors.lastname: Franco
authors.forename: Helena Bonciani
authors.initials: HB
authors.lastname: Nader
authors.forename: Hugo Alexandre Oliveira
authors.initials: HA
authors.lastname: Rocha
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D016466:CHO Cells; D002453:Cell Cycle / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D055808:Drug Discovery; D004789:Enzyme Activation / Q000187:drug effects; D005353:Fibronectins / Q000378:metabolism; D005643:Fucose / Q000737:chemistry; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D005353:Fibronectins; D011134:Polysaccharides; D005643:Fucose; D020928:Mitogen-Activated Protein Kinases
keywords: Antiproliferative; Cancer; Fucoidan; MAPK pathway