title: A graphene oxide-based platform for the assay of RNA synthesis by RNA polymerase using a fluorescent peptide nucleic acid probe.
abstract: We report a simple, direct fluorometric assay based on graphene oxide (GO) for RNA polymerase-mediated RNA synthesis. In principle, fluorescent peptide nucleic acid (PNA) probes were designed, and annealed with RNA products and the resultant RNA-PNA hybrids induced the recovery of fluorescence intensity of the PNA probes adsorbed onto the GO surface.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea. kimde@konkuk.ac.kr.
authors.forename: Joon Soo
authors.initials: JS
authors.lastname: Park
authors.forename: Ahruem
authors.initials: A
authors.lastname: Baek
authors.forename: Il-Soo
authors.initials: IS
authors.lastname: Park
authors.forename: Bong-Hyun
authors.initials: BH
authors.lastname: Jun
authors.forename: Dong-Eun
authors.initials: DE
authors.lastname: Kim
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005470:Fluorometry; D006108:Graphite / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D010087:Oxides / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000737:chemistry / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D010087:Oxides; D020135:Peptide Nucleic Acids; D012313:RNA; D006108:Graphite; D012321:DNA-Directed RNA Polymerases