title: Lutein extends the lifespan of Drosophila melanogaster.
abstract: Lutein is one of the major carotenoids in most fruits and vegetables. The effect of lutein on the lifespan of Drosophila melanogaster was investigated. Results revealed that 0.1mg lutein/ml diet could prolong their mean lifespan from 49.0 to 54.6 days. This was consistent with a significant reduction in malonyldialdehyde (MDA) level and increase in antioxidant enzyme activities of the flies fed with lutein-treated diet compared with those fed with basal diet. Paraquat (PQ) and H2O2 treatment tests demonstrated that lutein could prolong the survival time of the flies. Real-time polymerase chain reaction (RT-PCR) analysis indicated the gene expression of superoxide dismutase (SOD; SOD1 and SOD2), and catalase (CAT) in the lutein-treated group was up-regulated relative to that of the control group. It was concluded that the lifespan-prolonging activity of lutein was partially by up-regulation of endogenous antioxidant enzymes.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science & Technology, Tianjin 300457, China. Electronic address: zhangzesheng@tust.edu.cn.
authors.forename: Zesheng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Shunkai
authors.initials: S
authors.lastname: Han
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Tingting
authors.initials: T
authors.lastname: Wang
mesh_terms: D000818:Animals; D002374:Catalase / Q000096:biosynthesis / Q000235:genetics; D004331:Drosophila melanogaster / Q000187:drug effects / Q000502:physiology*; D005500:Follow-Up Studies; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008136:Longevity / Q000187:drug effects* / Q000502:physiology; D014975:Lutein / Q000494:pharmacology*; D008297:Male; D012313:RNA / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000235:genetics; D015854:Up-Regulation
chemical_list: D012313:RNA; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D014975:Lutein
keywords: AMD; CAT; Catalase; Drosophila melanogaster; Lifespan; Lutein; MDA; Malonyldialdehyde; SOD1; SOD2; Superoxide dismutase; age-related macular degeneration; catalase; copper–zinc SOD; malonyldialdehyde; manganese SOD