title: Promotion of cancer metastasis: candidate validation using an iTRAQ-based approach.
abstract: Evaluation of: Ghosh D, Li Z, Tan XF, Lim TK, Mao Y, Lin Q. RAQ based quantitative proteomics approach validated the role of calcyclin binding protein (CacyBP) in promoting colorectal cancer metastasis. Mol. Cell Proteomics 12(7), 1865-1880 (2013). The use of iTRAQ-based relative quantification is demonstrated by Ghosh et al. to analyze the downstream effectors of a calcyclin-binding protein, which is proposed to promote colorectal cancer progression. These findings are reviewed and discussed in relation to the need to establish robust in vitro model cell lines for identification of cancer-specific pathways and to confirm the leads generated by proteomic analysis.
authors.affiliation: ChELSI Institute, Department of Chemical and Biological Engineering, University of Sheffield, Mappin Street, Sheffield, S1 3JD, UK.
authors.forename: Caroline
authors.initials: C
authors.lastname: Evans
authors.forename: Bernard
authors.initials: B
authors.lastname: Corfe
mesh_terms: D002135:Calcium-Binding Proteins / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism*; D006801:Humans; D009362:Neoplasm Metastasis
chemical_list: D002135:Calcium-Binding Proteins