title: Mechanistic studies of sanguinamide B derivatives: a unique inhibitor of eukaryotic ribosomes.
abstract: Described are mechanistic studies of two Sanguinamide B (San B) derivatives. These compounds were identified as eukaryotic ribosomal inhibitors. Two biotinylated San B derivatives were synthesized and used to capture protein targets in a pull-down assay. LC/MS/MS analysis of the San B-captured targets identified several proteins that comprise eukaryotic ribosomal subunits. The translation inhibitory effect of San B was confirmed using an in vitro translation assay. Moreover, an evaluation of cell death mechanisms is reported.
authors.affiliation: School of Chemistry, The University of New South Wales , Kensington, NSW 2052 Australia.
authors.forename: Worawan
authors.initials: W
authors.lastname: Tantisantisom
authors.forename: Deborah M
authors.initials: DM
authors.lastname: Ramsey
authors.forename: Shelli R
authors.initials: SR
authors.lastname: McAlpine
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D006801:Humans; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010080:Oxazoles / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D010456:Peptides, Cyclic / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects* / Q000378:metabolism; D013844:Thiazoles / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology
chemical_list: D010080:Oxazoles; D010456:Peptides, Cyclic; D011506:Proteins; D013844:Thiazoles; C572270:sanguinamide B