title: Molecular characterization of Klebsiella pneumoniae carbapenemase-producing isolates in southern Brazil.
abstract: Carbapenem-resistant Enterobacteriaceae have been frequently reported worldwide. They represent a serious concern because of the limited therapeutic options. The aim of this study was to investigate the molecular epidemiology of 14 Klebsiella pneumoniae carbapenemase (KPC) producers among 345 clinical isolates of Enterobacteriaceae with reduced susceptibility to carbapenems recovered from 11 separate hospitals in southern Brazil. The blaKPC-2 gene was detected in 14 isolates (4 %): six Enterobacter cloacae, five K. pneumoniae and three Serratia marcescens. Most of these isolates exhibited high-level resistance against β-lactams and ciprofloxacin, while the most active drugs were polymyxin B and amikacin. Genetic environment analysis, based on the classical Tn4401 structure, revealed six distinct platforms. Plasmids carrying blaKPC-2 were not typable and most were approximately 20 kb. Only KPC carbapenemases were found among the isolates studied, highlighting the local relevance of these enzymes in acquired resistance to carbapenems in Enterobacteriaceae. Our results contribute to the understanding of carbapenem resistance in Enterobacteriaceae and to the molecular characterization of KPC-2-producing isolates in Brazil.
authors.affiliation: Unidade de Microbiologia e Biologia Molecular, Serviço de Patologia Clínica, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, Brazil.|Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
authors.forename: Vanessa Bley
authors.initials: VB
authors.lastname: Ribeiro
authors.affiliation: Universidade de São Paulo, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Ribeirão Preto, Brazil.
authors.forename: Leonardo Neves
authors.initials: LN
authors.lastname: Andrade
authors.affiliation: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
authors.forename: Adriano Rostirolla
authors.initials: AR
authors.lastname: Linhares
authors.affiliation: Programa de Pós-Graduação em Ciências Médicas, Faculdade de Medicina, UFRGS, Porto Alegre, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Barin
authors.affiliation: Universidade de São Paulo, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Ribeirão Preto, Brazil.
authors.forename: Ana Lúcia da Costa
authors.initials: ALDC
authors.lastname: Darini
authors.affiliation: Infectious Diseases Service, HCPA, Porto Alegre, Brazil.
authors.forename: Alexandre P
authors.initials: AP
authors.lastname: Zavascki
authors.affiliation: Unidade de Microbiologia e Biologia Molecular, Serviço de Patologia Clínica, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, Brazil.|Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
authors.forename: Afonso Luis
authors.initials: AL
authors.lastname: Barth
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001938:Brazil / Q000453:epidemiology; D015780:Carbapenems / Q000494:pharmacology; D016972:Enterobacter cloacae; D005838:Genotype; D006761:Hospitals; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D017720:Molecular Epidemiology; D012706:Serratia marcescens / Q000201:enzymology / Q000235:genetics; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases; C063912:carbapenemase