title: Citrate inhibition of cisplatin reaction with DNA studied using fluorescently labeled oligonucleotides: implication for selectivity towards guanine.
abstract: The reaction between cisplatin and DNA is conveniently studied using fluorescently labeled oligonucleotides and gel electrophoresis; as an example of application, the inhibition of this reaction by citrate is demonstrated, which might increase selectivity of cisplatin towards guanine over adenine.
authors.affiliation: Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada. liujw@uwaterloo.ca.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Po-Jung Jimmy
authors.initials: PJ
authors.lastname: Huang
authors.forename: Juewen
authors.initials: J
authors.lastname: Liu
mesh_terms: D002945:Cisplatin / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000494:pharmacology*; D019343:Citric Acid / Q000494:pharmacology; D004247:DNA / Q000187:drug effects*; D004591:Electrophoresis, Polyacrylamide Gel; D005452:Fluoresceins / Q000737:chemistry*; D006147:Guanine / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D005452:Fluoresceins; D009841:Oligonucleotides; D019343:Citric Acid; D006147:Guanine; D004247:DNA; D002945:Cisplatin