title: Fluorescence turn-on detection of a protein using cytochrome c as a quencher.
abstract: A new approach for the sensitive and selective sensing of a protein has been developed. Cytochrome c could quench the fluorescence of a fluorophore labeled aptamer efficiently. The specific binding between the aptamer and the target protein resulted in a turn-on emission signal, which could be used for protein quantification.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.
authors.forename: Dongli
authors.initials: D
authors.lastname: Liao
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Wenying
authors.initials: W
authors.lastname: Li
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Zhang
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Wang
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Li
authors.forename: Cong
authors.initials: C
authors.lastname: Yu
mesh_terms: D045304:Cytochromes c / Q000737:chemistry*; D057230:Limit of Detection; D011506:Proteins / Q000032:analysis*; D013050:Spectrometry, Fluorescence
chemical_list: D011506:Proteins; D045304:Cytochromes c