title: Preliminary characterization and immunostimulatory activity of polysaccharides from Glossaulax didyma.
abstract: In the present study, we investigated the preliminary characterization, both in vitro and in vivo immunostimulatory activity of polysaccharides from Glossaulax didyma (GPS). The results of chemical and gas chromatography analysis indicated that GPS was mainly composed of glucose with high contents of uronic acid and sulfate. For immunostimulatory activity in vitro, GPS could stimulate the proliferation of splenocytes and enhance neutral red phagocytosis, TNF-α production level and acid phosphatase activity in mouse peritoneal macrophages. For immunostimulatory activity in vivo, the administration of GPS could significantly increase the level of serum hemolysin in cyclophosphamide-treated mice. The results provided evidences that GPS possessed significant immunostimulatory activity and might be one of important active substances responsible for the immunostimulatory effect of G. didyma.
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Changxing
authors.initials: C
authors.lastname: Jiang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.forename: Songlin
authors.initials: S
authors.lastname: Li
authors.forename: Xirong
authors.initials: X
authors.lastname: Zhao
authors.forename: Qianghua
authors.initials: Q
authors.lastname: Zhang
authors.forename: Qingping
authors.initials: Q
authors.lastname: Xiong
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005260:Female; D049851:Gastropoda / Q000737:chemistry; D053878:Hemolytic Agents / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism; D008813:Mice, Inbred ICR; D009005:Monosaccharides / Q000032:analysis; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000276:Adjuvants, Immunologic; D053878:Hemolytic Agents; D009005:Monosaccharides; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D000135:Acid Phosphatase
keywords: Characterization; Glossaulax didyma; Immunostimulatory activity; Polysaccharide