title: Markers of angiogenesis (CD31, CD34, rCBV) and their prognostic value in low-grade gliomas.
abstract: OBJECTIVE
  Antigens CD31 and CD34 and relative cerebral blood volume (rCBV) in gliomas reflect in different ways neoangiogenesis of the tumour. Thus, we decided: (1) to estimate the correlation between the values of CD31 and CD34 and the value of rCBV in low-grade gliomas (LGG), and (2) to establish the prognostic value of these markers.
  
  
  METHODS
  The investigated group consisted of 53 patients with LGG who were operated on in the Neurosurgical Department at Sosnowiec between 2005 and 2011. On the basis of perfusion-weighted imaging (PWI-MRI) in the tumour texture, rCBV was calculated. The values of CD31 and CD34 were estimated on the basis of immunohistochemical investigation. Three outcome measures were assessed: (1) overall survival, (2) progression-free survival, and (3) malignant-free survival. Statistical analyses were done using the STATISTICA 9.0 program.
  
  
  RESULTS
  Higher value of rCBV in the texture of LGG significantly correlated with higher CD31 (p = 0.0006) and CD34 values (p = 0.0043). Progression-free survival was significantly longer in patients with rCBV < 1.75 than for persons with rCBV > 1.75 (p = 0.015). Lower expression of CD31 correlated with probability of longer survival of the patients after the operation of LGG (p = 0.068).
  
  
  CONCLUSIONS
  Density of microvessels as assessed immunohistochemically with CD31+ and CD34+ in LGG correlated with the value of rCBV in the tumour. The value of 1.75 for rCBV may be the threshold for better or poorer outcome of these patients. Expression of CD31 antigen is an important prognostic factor for the time of survival for patients with LGG.
authors.affiliation: Katedra i Oddział Kliniczny Neurochirurgii, Sosnowiec. wkaspera@wp.pl
authors.forename: K
authors.initials: K
authors.lastname: Majchrzak
authors.forename: W
authors.initials: W
authors.lastname: Kaspera
authors.forename: J
authors.initials: J
authors.lastname: Szymaś
authors.forename: B
authors.initials: B
authors.lastname: Bobek-Billewicz
authors.forename: A
authors.initials: A
authors.lastname: Hebda
authors.forename: H
authors.initials: H
authors.lastname: Majchrzak
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001810:Blood Volume; D001921:Brain / Q000098:blood supply / Q000503:physiopathology; D001932:Brain Neoplasms / Q000098:blood supply* / Q000401:mortality / Q000503:physiopathology; D002560:Cerebrovascular Circulation; D018572:Disease-Free Survival; D005910:Glioma / Q000098:blood supply* / Q000401:mortality / Q000503:physiopathology; D006801:Humans; D007150:Immunohistochemistry; D008279:Magnetic Resonance Imaging; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism*; D011379:Prognosis; D015996:Survival Rate
chemical_list: D018952:Antigens, CD34; D014408:Biomarkers, Tumor; D019408:Platelet Endothelial Cell Adhesion Molecule-1