title: IL-13 gene polymorphisms and their association with atopic asthma and rhinitis in Pakistani patients.
abstract: Interleukin-13 (IL-13) is known to be a key regulator in immunoglobulin E (IgE) synthesis, mucus hypersecretion and airway hyperresponsiveness. Single nucleotide polymorphisms in IL-13 are associated with allergic phenotypes in several ethnically diverse populations.This study was performed in 214 atopic patients (asthma n=108, allergic rhinitis n=106) and sex-matched healthy controls (n=120). Genotyping of IL-13 gene polymorphisms was performed using polymerase chain reaction-based restriction fragment length polymorphism method.A statistically significant association of the A-1512C polymorphism in IL13 gene was observed with atopy (p&lt;0.001; χ2=19.0). Upon stratification of the data into asthma and AR association was revealed with both asthma (p=0.01; χ2=8.80) and AR (p&lt;0.001; χ2=24.3) in Pakistani patients. Higher odds ratio (OR 95% CI) was observed for AR 3.42 (2.04-5.76) relative to asthma 2.40 (1.41-4.09) for the C allele compared to controls.In conclusion the study provides the evidence A-1512C polymorphisms in IL-13 is a risk factor for asthma and AR.
authors.affiliation: Department of Biosciences, COMSATS Institute of Information Technology, Islamabad-44000, Pakistan and Department of Ophthalmology, Radboud University Nijmegen Medical Centre; Nijmegen, Netherlands. shaziamicheal@gmail.com.
authors.forename: Micheal
authors.initials: M
authors.lastname: Shazia
authors.forename: Minhas
authors.initials: M
authors.lastname: Kanza
authors.forename: Ishaque
authors.initials: I
authors.lastname: Mehwish
authors.forename: Shabbir
authors.initials: S
authors.lastname: Irum
authors.forename: Ahmed
authors.initials: A
authors.lastname: Farida
authors.forename: Ahmed
authors.initials: A
authors.lastname: Asifa
mesh_terms: D001249:Asthma / Q000209:etiology / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D018793:Interleukin-13 / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide; D065631:Rhinitis, Allergic; D012221:Rhinitis, Allergic, Perennial / Q000209:etiology / Q000235:genetics*
chemical_list: C578095:IL13 protein, human; D018793:Interleukin-13