title: Quantitative proteomics of extracellular vesicles derived from human primary and metastatic colorectal cancer cells.
abstract: Cancer cells actively release extracellular vesicles (EVs), including exosomes and microvesicles, into surrounding tissues. These EVs play pleiotropic roles in cancer progression and metastasis, including invasion, angiogenesis, and immune modulation. However, the proteomic differences between primary and metastatic cancer cell-derived EVs remain unclear. Here, we conducted comparative proteomic analysis between EVs derived from human primary colorectal cancer cells (SW480) and their metastatic derivatives (SW620). Using label-free quantitation, we identified 803 and 787 proteins in SW480 EVs and SW620 EVs, respectively. Based on comparison between the estimated abundance of EV proteins, we identified 368 SW480 EV-enriched and 359 SW620 EV-enriched proteins. SW480 EV-enriched proteins played a role in cell adhesion, but SW620 EV-enriched proteins were associated with cancer progression and functioned as diagnostic indicators of metastatic cancer; they were overexpressed in metastatic colorectal cancer and played roles in multidrug resistance. As the first proteomic analysis comparing primary and metastatic cancer-derived EVs, this study increases our understanding of the pathological function of EVs in the metastatic process and provides useful biomarkers for cancer metastasis.
authors.affiliation: Department of Life Science and Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
authors.forename: Dong-Sic
authors.initials: DS
authors.lastname: Choi
authors.forename: Do-Young
authors.initials: DY
authors.lastname: Choi
authors.forename: Bok Sil
authors.initials: BS
authors.lastname: Hong
authors.forename: Su Chul
authors.initials: SC
authors.lastname: Jang
authors.forename: Dae-Kyum
authors.initials: DK
authors.lastname: Kim
authors.forename: Jaewook
authors.initials: J
authors.lastname: Lee
authors.forename: Yoon-Keun
authors.initials: YK
authors.lastname: Kim
authors.forename: Kwang Pyo
authors.initials: KP
authors.lastname: Kim
authors.forename: Yong Song
authors.initials: YS
authors.lastname: Gho
keywords: APEX; biomarker; colorectal cancer; ectosomes; exosomes; label-free quantitative proteomics; metastasis; microvesicles; nanoparticle tracking analysis; secretome