title: Significant modulation of macrophages associated cytokines TNF-α, VEGF and apoptotoic protein Bax, Bcl2 abrogates tumor cells.
abstract: T11 target structure (T11TS), a membrane glycoprotein has been documented with anti neoplastic activity in glioma bearing animal model in our lab. In this study, we have evaluated the phagocytic potential, expression of VEGF, TNF-α in T11TS treated and untreated macrophages in all four grades of glioma. The data indicates the significant enhancement of phagocytosis in T11TS treated macrophages of grades I and II glioma. There was significant up regulation in TNF-α and significant down regulation in VEGF expression in T11TS treated macrophages in grade I and II glioma. We also attempted to know any possible apoptotic role of T11TS in tumor cells by comparing Bax and Bcl2 in treated and untreated tumor cells of all four grades. We found significant up regulation in Bax expression and down regulation in Bcl2 expression of grades I and II glioma. The outcome may help in pushing this molecule into pharmaceutical domain.
authors.affiliation: Department of Laboratory Medicine, School of Tropical Medicine, 108 Chittaranjan Avenue, Kolkata 700 073, India. Electronic address: pankajvatsa2002@gmail.com.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Kumar
authors.forename: Sirshendu
authors.initials: S
authors.lastname: Chatterjee
authors.forename: Sagar
authors.initials: S
authors.lastname: Acharya
authors.forename: Annpurna
authors.initials: A
authors.lastname: Kumari
authors.forename: Suhnrita
authors.initials: S
authors.lastname: Chaudhuri
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Singh
authors.forename: Samarendra Nath
authors.initials: SN
authors.lastname: Ghosh
authors.forename: Swapna
authors.initials: S
authors.lastname: Chaudhuri
mesh_terms: D000293:Adolescent; D000328:Adult; D017209:Apoptosis / Q000276:immunology; D001932:Brain Neoplasms / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology; D018801:CD2 Antigens / Q000276:immunology / Q000494:pharmacology*; D002219:Carbamates / Q000627:therapeutic use*; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D005910:Glioma / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D042461:Vascular Endothelial Growth Factor A / Q000276:immunology; D055815:Young Adult; D051028:bcl-2-Associated X Protein / Q000276:immunology
chemical_list: D018801:CD2 Antigens; D002219:Carbamates; D014409:Tumor Necrosis Factor-alpha; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D051028:bcl-2-Associated X Protein; C492029:transkarbam 12
keywords: Cytokine; HIF; Immunomodulation; Macrophages; Phagocytosis; T11 target structure; T11TS; TNF; VEGF; hypoxia inducible factor; tumor necrosis factor; vascular endothelial growth factor