title: Interleukin-1 gene polymorphisms in chronic gastritis patients infected with Helicobacter pylori as risk factors of gastric cancer development.
abstract: Epidemiological investigations indicated association of the Helicobacter pylori infections with the occurrence of inflammatory conditions of the gastric mucosa and development of chronic gastritis and intestinal type of gastric cancer. IL1A and IL1B genes have been proposed as key factors in determining risk of gastritis and malignant transformation. The aim of this paper was to evaluate association of interleukin-1 gene polymorphisms with chronic gastritis, atrophy, intestinal metaplasia, dysplasia and intestinal type of gastric cancer in H. pylori-infected patients. Patients subjected to analysis represent group of 144 consecutive cases that suffered from dyspepsia with coexisting infection of H. pylori and chronic gastritis, chronic atrophic gastritis, intestinal metaplasia, dysplasia or gastric cancer. Molecular studies involved analysis of -889C>T polymorphism of IL1A gene and +3954C>T polymorphism of IL1B gene. Statistical analysis of association of polymorphism -889C>T of gene IL1A with changes in gastric mucosa showed lack of significance, whereas +3954C>T polymorphism of IL1B gene showed significant association. Frequency of allele T of +3954C>T polymorphism of IL1B gene was higher in group of patients with chronic gastritis, atrophy, intestinal metaplasia, dysplasia or intestinal type of gastric cancer (32.1 %) as compared with population group (23 %), χ(2) = 4.61 and p = 0.03. This corresponds to odds ratio: 1.58, 95 % CI: 1.04-2.4. Our results indicate that +3954C>T polymorphism of IL1B gene increase susceptibility to inflammatory response of gastric mucosa H. pylori-infected patients and plays a significant role in the development of chronic gastritis, atrophy, intestinal metaplasia, dysplasia and the initiation of carcinogenesis.
authors.affiliation: Department of Internal Medicine, District Hospital, Drawsko Pomorskie, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Hnatyszyn
authors.forename: Karolina
authors.initials: K
authors.lastname: Wielgus
authors.forename: Marta
authors.initials: M
authors.lastname: Kaczmarek-Rys
authors.forename: Marzena
authors.initials: M
authors.lastname: Skrzypczak-Zielinska
authors.forename: Marlena
authors.initials: M
authors.lastname: Szalata
authors.forename: Joanna
authors.initials: J
authors.lastname: Mikolajczyk-Stecyna
authors.forename: Jerzy
authors.initials: J
authors.lastname: Stanczyk
authors.forename: Ireneusz
authors.initials: I
authors.lastname: Dziuba
authors.forename: Adam
authors.initials: A
authors.lastname: Mikstacki
authors.forename: Ryszard
authors.initials: R
authors.lastname: Slomski
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D063646:Carcinogenesis / Q000235:genetics; D002277:Carcinoma / Q000276:immunology*; D004252:DNA Mutational Analysis; D005756:Gastritis / Q000276:immunology*; D005787:Gene Frequency; D056726:Genetic Association Studies; D005838:Genotype; D016481:Helicobacter Infections / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D007413:Intestinal Mucosa / Q000473:pathology*; D008297:Male; D011110:Polymorphism, Genetic; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000276:immunology*; D055815:Young Adult
chemical_list: D007375:Interleukin-1