title: A 5-fluorouracil-loaded polydioxanone weft-knitted stent for the treatment of colorectal cancer.
abstract: In-stents restenosis caused by tumour ingrowth is a major problem for patients undergoing stent displacement because the conventional stents often lack a sustained anti-tumour capability. The aim of this paper was to develop a weft-knitted polydioxanone stent which can slow release 5-fluorouracil (5-FU). In order to determine the most suitable drug concentration, the 5-FU safe concentration in vivo and appropriate loading percentage in the membranes were investigated, and then 5-FU-loaded poly-l-lactide membranes at concentration of 3.2%, 6.4% and 12.8% were coated onto the stent using electro-spinning method, respectively. The morphology, chemical structure and in vitro drug release property of the coating membranes were subsequently examined. Their anti-tumour activity and mechanism were assessed in vitro and in vivo using a human colorectal cancer cell line HCT-116 and tumour-bearing BALB/c nude mice. The half maximal inhibitory concentration (IC50) and the median lethal dose (LD50) demonstrated that the 6.4% and 12.8% membranes had better anti-tumour effects than pure 5-FU due to the sustainable drug releasing property of the coated membranes on the stent. The membranes possessing appropriate drug loading doses, such as 6.4% or 12.8% also provided better anti-in-stents restenosis effects than other groups tested. Therefore, it is concluded that the drug-loaded stents have great potential for the use in the treatment of intestinal cancers in the future.
authors.affiliation: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Kowloon, Hong Kong; Guangdong-HK International Textile Bioengineering Joint Research Center, The Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen, PR China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Wu
authors.forename: Junyan
authors.initials: J
authors.lastname: Hu
authors.forename: Xiaowen
authors.initials: X
authors.lastname: He
authors.forename: Jiashen
authors.initials: J
authors.lastname: Li
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhao
authors.forename: Zexian
authors.initials: Z
authors.lastname: Chen
authors.forename: Yuling
authors.initials: Y
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Hu
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Lan
mesh_terms: D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage* / Q000627:therapeutic use; D003106:Colon / Q000187:drug effects / Q000473:pathology; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D054855:Drug-Eluting Stents; D005472:Fluorouracil / Q000008:administration & dosage* / Q000627:therapeutic use; D045325:HCT116 Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016687:Polydioxanone / Q000737:chemistry*; D012007:Rectum / Q000187:drug effects / Q000473:pathology; D015607:Stents
chemical_list: D000964:Antimetabolites, Antineoplastic; D016687:Polydioxanone; D005472:Fluorouracil
keywords: 5-FU-loaded weft-knitted stents; Anti-tumour effect; Drug release; Intestinal stenosis; In vitro and in vivo