title: Multiple complexation of cyclodextrin with soy isoflavones present in an enriched fraction.
abstract: In the present study we evaluated the complexation of daidzein/genistein/glycitein, present in an isoflavone enriched fraction (IEF), with β-cyclodextrin and 2-hydroxypropyl-β-cyclodextrin (HPβCD). Based on the increased solubility and higher complexation efficiency, IEF and HPβCD solid complexes were prepared by kneading, freeze-drying, co-evaporation, spray-drying and microwave. The solid complexes were characterized using Fourier transformed-infrared spectroscopy, differential scanning calorimetry, X-ray diffraction, scanning electron microscopy, and nuclear magnetic resonance spectroscopy, and the isoflavone content and solubility were determined by liquid chromatography. The results suggest that the isoflavones daidzein, genistein and glycitein may be externally associated to HPβCD as well as that isoflavones/HPβCD inclusion complexes are formed through the insertion of B-ring into the cyclodextrin cavity. Except for the freeze-dried IEF/HPβCD solid complex, all complexes showed similar content and solubility. In conclusion, the three isoflavones showed to be able to simultaneously complex with HPβCD.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752, CEP 90610-000 Porto Alegre, RS, Brazil. franciniyatsu@gmail.com
authors.forename: Francini K J
authors.initials: FK
authors.lastname: Yatsu
authors.forename: Letícia S
authors.initials: LS
authors.lastname: Koester
authors.forename: Ivana
authors.initials: I
authors.lastname: Lula
authors.forename: Joel J
authors.initials: JJ
authors.lastname: Passos
authors.forename: Rubén
authors.initials: R
authors.lastname: Sinisterra
authors.forename: Valquiria L
authors.initials: VL
authors.lastname: Bassani
mesh_terms: D003505:Cyclodextrins / Q000737:chemistry*; D007529:Isoflavones / Q000737:chemistry*; D012995:Solubility; D013025:Glycine max / Q000737:chemistry*
chemical_list: D003505:Cyclodextrins; D007529:Isoflavones
keywords: Cyclodextrin; Daidzein; Genistein; Glycitein; Isoflavones