title: Functionalization of quartz crystal microbalances with liposomes containing the N-hydroxysuccinimide ester of palmitic acid.
abstract: We developed a fast and simple method to functionalize a quartz crystal microbalance (QCM) with liposomes composed of phosphatidylcholine lipid and the N-hydroxysuccinimide (NHS) ester of palmitic acid. The liposome was applied directly to a bare gold surface of a QCM to prepare the lipid bilayer presenting NHS groups on the surface. The whole functionalization process was completed within 1 h using stored lipid films. Streptavidin immobilization efficiency of the method was comparable to the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)/NHS chemical method, and the activity of the biotinylated antibody immobilized through the streptavidin was stably retained in repeated surface regeneration with the dissociation buffer.
authors.affiliation: Department of Chemistry, Gangneung-Wonju National University, Gangneung, Gangwondo 210-702, Republic of Korea.
authors.forename: Sora
authors.initials: S
authors.lastname: Eom
authors.forename: Eunkyeong
authors.initials: E
authors.lastname: Yu
authors.forename: Suk-Jung
authors.initials: SJ
authors.lastname: Choi
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D019921:Biotinylation; D006041:Goats; D006046:Gold; D055767:Immobilized Proteins / Q000737:chemistry; D007074:Immunoglobulin G / Q000032:analysis*; D008081:Liposomes / Q000737:chemistry*; D010169:Palmitic Acids / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry; D058525:Quartz Crystal Microbalance Techniques / Q000295:instrumentation* / Q000379:methods; D012680:Sensitivity and Specificity; D019809:Streptavidin / Q000737:chemistry; D013388:Succinimides / Q000737:chemistry*
chemical_list: D055767:Immobilized Proteins; D007074:Immunoglobulin G; D008081:Liposomes; D010169:Palmitic Acids; D010713:Phosphatidylcholines; D013388:Succinimides; D006046:Gold; D019809:Streptavidin; C001426:N-hydroxysuccinimide
keywords: Biosensor; Functionalization; Liposome; N-Hydroxysuccinimide; Quartz crystal microbalance (QCM)