title: [Role of the surgical pathologist for tissue management in oncology].
abstract: Currently, the increasing number of ancillary methods to be performed from tumoral tissues in a pathology laboratory determines the necessity to have an optimal strategy for tissue management. The size of tissue samples dedicated for a pathological examination becomes smaller and smaller, as the diagnosis can be made with non or less invasive methods. However, the samples should also allow to provide the prognosis as well as to realise biological molecular testing in order to found a genomic alteration. Thus, it is critical to think about how to share and to pool the different expertises and abilities in a pathology laboratory in order to optimize the achievement of the different ancillary methods. Thus, following the morphological study made in hematoxylin-eosin staining, it is necessary to preempt the number of immunohistochemical and in situ hybridization studies, which will be potentially done from the tissue samples. Moreover, since the genomic alteration detection in tumours is mainly performed from DNA extracted from tissues, it is necessary to take in account some numerous parameters, in particular the nature and the time of fixation, the percentage of tumour cells, the presence of necrotic area, the percentage of inflammatory cells and the sample size. The strategy for an optimal tissue management in an oncology-pathology laboratory is critical and takes part of the different steps allowing to get an accreditation according the ISO15189 norm.
authors.affiliation: Centre hospitalo-universitaire de Nice (CHUN), hôpital Pasteur, université de Nice - Sophia-Antipolis, laboratoire de pathologie clinique et expérimentale et biobanque du CHUN, 30, avenue de la Voie-Romaine, 06002 Nice cedex 01, France.
authors.forename: Élodie
authors.initials: É
authors.lastname: Long
authors.forename: Marius
authors.initials: M
authors.lastname: Ilie
authors.forename: Véronique
authors.initials: V
authors.lastname: Hofman
authors.forename: Sandra
authors.initials: S
authors.lastname: Lassalle
authors.forename: Catherine
authors.initials: C
authors.lastname: Butori
authors.forename: Saad
authors.initials: S
authors.lastname: Alsubaie
authors.forename: Paul
authors.initials: P
authors.lastname: Hofman
mesh_terms: D015925:Cryopreservation / Q000379:methods / Q000592:standards; D004273:DNA, Neoplasm / Q000032:analysis; D019983:Guideline Adherence / Q000592:standards; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods / Q000592:standards; D017403:In Situ Hybridization / Q000379:methods / Q000592:standards; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D010340:Pathology, Surgical / Q000379:methods* / Q000592:standards; D012334:RNA, Neoplasm / Q000032:analysis; D012380:Role; D013048:Specimen Handling / Q000379:methods* / Q000592:standards; D013194:Staining and Labeling / Q000379:methods / Q000592:standards; D016707:Tissue Fixation / Q000379:methods* / Q000592:standards
chemical_list: D004273:DNA, Neoplasm; D012334:RNA, Neoplasm
keywords: biomarker; biopsy; fixative; frozen procedure; immunohistochemistry; in situ hybridization; molecular biology