title: Effects of polishing methods on Candida albicans adhesion on cast pure titanium surfaces.
abstract: OBJECTIVE
  To explore the effects of different polishing methods on Candida albicans adhesion to cast pure titanium surfaces.
  
  
  METHODS
  Pure titanium test specimens randomly divided into 4 groups: not specially treated (CT), manual polishing (MPT), electrolytic polishing (EPT), and centrifugal barrel polishing (CPT). Surface roughness was measured with surface profilometer. Contact angles of water, diiodomethane, and ethylene glycol on specimen surface were measured, and the free energy was calculated. Each group were immersed in a C. albicans suspension for 48 hours, and cell count of C. albicans adhesion were taken. Morphology of C. albicans adhesion was observed by SEM.
  
  
  RESULTS
  Surface roughness (Ra) were in the following order: CT > EPT > MPT > CPT. The amount of C. albicans adhesion to the surface of CPT was significantly less than CT, MPT, and EPT by cells count (P < 0.05). In SEM, less C. albicans aggregation formed on the surface of CPT than other 3 groups.
  
  
  CONCLUSIONS
  Specimens treated by the CPT method showed superior effect to the specimens treated with MPT and EPT methods in reducing the adhesion of C. albicans.
authors.affiliation: *Attending Physician, Department of Prosthodontics, Affiliated Stomatological Hospital of the Fourth Military Medical University, Xi'an, China. †Professor, Chief Physician, Department of Prosthodontics, Affiliated Stomatological Hospital of the Fourth Military Medical University, Xi'an, China. ‡Instructor, Attending Physician, Department of Oral Implantology, Affiliated Stomatological Hospital of Nanchang University, Nanchang, China. §Resident Physician, Department of Prosthodontics, Affiliated Stomatological Hospital of the Fourth Military Medical University, Xi'an, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Li
authors.forename: Tianwen
authors.initials: T
authors.lastname: Guo
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
mesh_terms: D002176:Candida albicans / Q000502:physiology*; D002448:Cell Adhesion; D015921:Dental Implants / Q000382:microbiology; D003775:Dental Polishing / Q000379:methods*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D013499:Surface Properties; D014025:Titanium
chemical_list: D015921:Dental Implants; D014025:Titanium