title: A modified microsurgical model for end-to-side selective portacaval shunt in the rat. Intraoperative microcirculatory investigations.
abstract: OBJECTIVE
  To investigate the intraoperative microcirculatory changes of the affected organs (small bowel, liver and kidney) during the making of a modified selective portacaval (PC) shunt.
  
  
  METHODS
  On ten anaesthetized Sprague-Dawley rats the selective end-to-side mesocaval anastomosis was performed, where only the rostral mesenteric vein is utilized and the portal vein with the splenic vein are left intact. Morphometric and microcirculatory investigations using a LDF device determining flux units (BFU) were carried out.
  
  
  RESULTS
  After completing the shunts the microcirculatory flux values did not recover in the same manner on the surface of the small intestine, the liver or the kidney. BFU values showed deterioration in the small intestine and in the liver (p<0.001). During the reperfusion the BFU values improved, but not in the same manner. The small intestine values left behind the kidney and liver data.
  
  
  CONCLUSIONS
  Technically, the advantages of the models include the selective characteristic, the mesocaval localization and the relatively easy access to those vessels. However, its major disadvantage is the time needed for positioning the vessels without coiling or definitive stretching. Intraoperative LDF may provide useful data on the microcirculatory affection of the organs suffering from hypoperfusion or ischemia during creating the shunts.
authors.affiliation: Department of Operative Techniques and Surgical Research, Institute of Surgery, Medical and Health Science Center, University of Debrecen, Hungary.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Klarik
authors.forename: Eniko
authors.initials: E
authors.lastname: Toth
authors.forename: Ferenc
authors.initials: F
authors.lastname: Kiss
authors.forename: Iren
authors.initials: I
authors.lastname: Miko
authors.forename: Istvan
authors.initials: I
authors.lastname: Furka
authors.forename: Norbert
authors.initials: N
authors.lastname: Nemeth
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D007432:Intraoperative Period; D008642:Mesenteric Veins / Q000033:anatomy & histology; D008833:Microcirculation / Q000502:physiology*; D008866:Microsurgery / Q000379:methods*; D023421:Models, Animal; D011167:Portacaval Shunt, Surgical / Q000379:methods*; D011169:Portal Vein / Q000033:anatomy & histology / Q000601:surgery*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D015203:Reproducibility of Results; D014682:Vena Cava, Inferior / Q000601:surgery*