title: Detection of S-nitrosothiols.
abstract: BACKGROUND
  S-nitrosothiols have been recognized as biologically-relevant products of nitric oxide that are involved in many of the diverse activities of this free radical.
  
  
  METHODS
  This review serves to discuss current methods for the detection and analysis of protein S-nitrosothiols. The major methods of S-nitrosothiol detection include chemiluminescence-based methods and switch-based methods, each of which comes in various flavors with advantages and caveats.
  
  
  CONCLUSIONS
  The detection of S-nitrosothiols is challenging and prone to many artifacts. Accurate measurements require an understanding of the underlying chemistry of the methods involved and the use of appropriate controls.
  
  
  CONCLUSIONS
  Nothing is more important to a field of research than robust methodology that is generally trusted. The field of S-nitrosation has developed such methods but, as S-nitrosothiols are easy to introduce as artifacts, it is vital that current users learn from the lessons of the past. This article is part of a Special Issue entitled Current methods to study reactive oxygen species - pros and cons and biophysics of membrane proteins. Guest Editor: Christine Winterbourn.
authors.affiliation: Department of Biophysics and Redox Biology Program, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors.forename: Anne R
authors.initials: AR
authors.lastname: Diers
authors.forename: Agnes
authors.initials: A
authors.lastname: Keszler
authors.forename: Neil
authors.initials: N
authors.lastname: Hogg
mesh_terms: D000818:Animals; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D026403:S-Nitrosothiols / Q000032:analysis*
chemical_list: D026403:S-Nitrosothiols
keywords: Chemiluminescence; Methodology; Proteomics; S-nitrosothiol