title: The effects of nasal irritant induced responses on breathing and cough in anaesthetized and conscious animal models.
abstract: There is little evidence to support the down-regulation of coughing from the nose. The cough response to citric acid (CA) was studied in anesthetized and conscious guinea pigs after nasal pretreatment with saline, 1% DMSO, allylisothiocyanate (TRPA1 agonist) and allylisothiocyanate +AP-18 (TRPA1 antagonist). Cough was induced by adding citric acid (CA) to the tracheal perfusion in anaesthetized animals, or by inhaling 0.4M CA in conscious animals. The cough response was counted from the dose response curves, airflow traces and cough sound analysis. In conscious animals, nasal allylisothiocyanate induced reproducible, dose dependent nasal symptoms and a significant drop in respiratory rate. Cough induced by CA was suppressed after nasal allylisothiocyanate (p<0.05), and this effect was prevented by AP-18 (1mM). In anaesthetized animals, nasal allylisothiocyanate induced a significant drop in respiratory rate. Cough induced subsequently by CA was suppressed when compared to baseline and vehicle responses (p<0.05). The reasons for the suppression of CA induced cough by TRPA1 agonist applied to the nose are not clear and remain to be elucidated.
authors.affiliation: Department of Pathophysiology, Comenius University, Jessenius Faculty of Medicine, Martin, Slovak Republic; Clinic of Anesthesiology and Emergency Medicne, University hospital in Martin, Comenius University, Jessenius Faculty of Medicine, Martin, Slovak Republic.
authors.forename: Z
authors.initials: Z
authors.lastname: Biringerova
authors.forename: S
authors.initials: S
authors.lastname: Gavliakova
authors.forename: M
authors.initials: M
authors.lastname: Brozmanova
authors.forename: M
authors.initials: M
authors.lastname: Tatar
authors.forename: E
authors.initials: E
authors.lastname: Hanuskova
authors.forename: I
authors.initials: I
authors.lastname: Poliacek
authors.forename: J
authors.initials: J
authors.lastname: Plevkova
mesh_terms: D000281:Administration, Intranasal; D000758:Anesthesia; D000818:Animals; D000925:Anticoagulants / Q000633:toxicity; D000996:Antitussive Agents / Q000008:administration & dosage; D019343:Citric Acid / Q000633:toxicity; D003243:Consciousness / Q000187:drug effects / Q000502:physiology*; D003371:Cough / Q000139:chemically induced / Q000503:physiopathology* / Q000517:prevention & control; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D017879:Isothiocyanates / Q000008:administration & dosage; D008297:Male; D012119:Respiration / Q000187:drug effects
chemical_list: D000925:Anticoagulants; D000996:Antitussive Agents; D017879:Isothiocyanates; D019343:Citric Acid; C041942:2,3,4-tri-O-acetylarabinopyranosyl isothiocyanate
keywords: Animal models; Cough; Regulation; TRPA1