title: The aflatoxin B1 isolating potential of two lactic acid bacteria.
abstract: OBJECTIVE
  To determine lactic acid bacteria's capability to enhance the process of binding and isolating aflatoxin B1 and to utilize such lactic acid bacteria as a food supplement or probiotic products for preventing absorption of aflatoxin B1 in human and animal bodies.
  
  
  METHODS
  In the present research, the bacteria were isolated from five different sources. For surveying the capability of the bacteria in isolating aflatoxin B1, ELISA method was implemented, and for identifying the resultant strains through 16S rRNA sequencing method, universal primers were applied.
  
  
  RESULTS
  Among the strains which were isolated, two strains of Lactobacillus pentosus and Lactobacillus beveris exhibited the capability of absorbing and isolating aflatoxin B1 by respectively absorbing and discharging 17.4% and 34.7% of the aforementioned toxin existing in the experiment solution.
  
  
  CONCLUSIONS
  Strains of Lactobacillus pentosus and Lactobacillus beveris were isolated from human feces and local milk samples, respectively. And both strains has the ability to isolate or bind with aflatoxin B1.
authors.affiliation: Department of Microbiology, Razi Vaccine and Serum Research Institute, Arak, Iran.
authors.forename: Adel
authors.initials: A
authors.lastname: Hamidi
authors.forename: Reza
authors.initials: R
authors.lastname: Mirnejad
authors.forename: Emad
authors.initials: E
authors.lastname: Yahaghi
authors.forename: Vahid
authors.initials: V
authors.lastname: Behnod
authors.forename: Ali
authors.initials: A
authors.lastname: Mirhosseini
authors.forename: Sajad
authors.initials: S
authors.lastname: Amani
authors.forename: Sara
authors.initials: S
authors.lastname: Sattari
authors.forename: Ebrahim Khodaverdi
authors.initials: EK
authors.lastname: Darian
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D006801:Humans; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008892:Milk / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D016604:Aflatoxin B1
keywords: Aflatoxin; Bacteria; ELISA test; Lactic acid; Mycotoxin