title: The antioxidant properties of solid-culture extracts of basidiomycetous fungi.
abstract: Current studies of the antioxidant activity of fungal resources are mainly focused on the fruiting bodies of edible mushrooms. To access the potential of basidiomycetes in culture-state applications, extracts of solid cultures of 83 basidiomycetous fungi newly isolated from woody materials were prepared at the same concentration (10 mg/ml), and their antioxidant activities were measured using ABTS (2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical-scavenging assays. Among the basidiomycetes tested, Cryptoporus volvatus, Daedalea dickinsii, Gloeophyllum abietinum, G. trabeum, Pseudomerulius curtisii and Stereum hirsutum exhibited good antioxidant activities. The EC50 value for the removal of free radicals was lowest (i.e., most effective) in the crude extract of S. sanguinolentum (19.61 g/ml), and reduced DNA damage based on a DNA nicking assay was observed for the extracts from the six basidiomycetous species above.
authors.affiliation: Division of Environmental Science & Ecological Engineering, Korea University, Seoul, Korea.
authors.forename: Jaejung
authors.initials: J
authors.lastname: Lee
authors.forename: Joo-Hyun
authors.initials: JH
authors.lastname: Hong
authors.forename: Jeong-Do
authors.initials: JD
authors.lastname: Kim
authors.forename: Byoung Jun
authors.initials: BJ
authors.lastname: Ahn
authors.forename: Beom Seok
authors.initials: BS
authors.lastname: Kim
authors.forename: Gyu-Hyeok
authors.initials: GH
authors.lastname: Kim
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Kim
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification* / Q000378:metabolism; D001487:Basidiomycota / Q000737:chemistry* / Q000254:growth & development*; D052160:Benzothiazoles / Q000378:metabolism; D001713:Biphenyl Compounds / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D010851:Picrates / Q000378:metabolism; D013451:Sulfonic Acids / Q000378:metabolism
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D003470:Culture Media; D010851:Picrates; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl