title: A Salmonella Enteritidis hilAssrAfliG deletion mutant is a safe live vaccine strain that confers protection against colonization by Salmonella Enteritidis in broilers.
abstract: Consumption of contaminated poultry meat is an important cause of Salmonella infections in humans. Therefore, there is a need for control methods that protect broilers from day-of-hatch until slaughter age against infection with Salmonella. Colonization-inhibition, a concept in which a live Salmonella strain is orally administered to day-old chickens and protects against subsequent challenge, can potentially be used as control method. In this study, the safety and efficacy of a Salmonella Enteritidis ΔhilAssrAfliG strain as a colonization-inhibition strain for protection of broilers against Salmonella Enteritidis was evaluated. After administration of the Salmonella Enteritidis ΔhilAssrAfliG strain to day-old chickens, this strain could not be isolated from the gut, internal organs or faeces after 21 days of age. In addition, administration of this strain to one-day-old broiler chickens decreased faecal shedding and caecal and internal organ colonization of a Salmonella Enteritidis challenge strain administered one day later using a seeder bird model. To our knowledge, this is the first report of an attenuated Salmonella strain for which both the safety and efficacy has been shown in long-term experiments (until slaughter age) in broiler strain can potentially be used as a live colonization-inhibition strain for controlling Salmonella Enteritidis infections in broilers.
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
authors.forename: W
authors.initials: W
authors.lastname: De Cort
authors.forename: S
authors.initials: S
authors.lastname: Geeraerts
authors.forename: V
authors.initials: V
authors.lastname: Balan
authors.forename: M
authors.initials: M
authors.lastname: Elroy
authors.forename: F
authors.initials: F
authors.lastname: Haesebrouck
authors.forename: R
authors.initials: R
authors.lastname: Ducatelle
authors.forename: F
authors.initials: F
authors.lastname: Van Immerseel
mesh_terms: D000818:Animals; D057138:Bacterial Shedding; D002432:Cecum / Q000382:microbiology; D002645:Chickens / Q000276:immunology* / Q000382:microbiology; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control*; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000517:prevention & control*; D022562:Salmonella Vaccines / Q000627:therapeutic use*; D012477:Salmonella enteritidis / Q000235:genetics* / Q000276:immunology; D017384:Sequence Deletion; D014611:Vaccination / Q000662:veterinary; D014613:Vaccines, Attenuated / Q000627:therapeutic use
chemical_list: D022562:Salmonella Vaccines; D014613:Vaccines, Attenuated
keywords: Colonization-inhibition; Poultry; Salmonella