title: [On-line monitoring of extraction process of danhong injection based on near-infrared spectroscopy].
abstract: OBJECTIVE
  To establish a rapid quantitative analysis method for the quality control of Danhong injection extraction using near-infrared (NIR) spectroscopy.
  
  
  METHODS
  Online collecting the NIR spectra during the mixed extraction process of Salvia miltiorrhiza and Carthamus tinctorius, partial least squares regression (PLSR) models were developed for the quality indicators rosmarinic acid (RA), salvia acid B (SaB), lithospermic acid (LA), hydroxysafflor yellow A (HSYA) and solid content (SSC), with HPLC and weight-loss method as reference methods.
  
  
  RESULTS
  The correlation coefficients of the cross validation for RA, SaB, LA, HSYA and SSC were 0.909 3, 0.915 2, 0.901 9, 0.747 7 and 0.931 4, respectively. And the root mean square errors of cross validation (RMSECV) were 0.012 1, 0.251, 0.017 7, 0.038 1 g x L(-1) and 0.359%, respectively.
  
  
  CONCLUSIONS
  In this study, NIR spectroscopy was successfully applied to achieve the real-time determination of the contents of RA, SaB, LA and SSC, while the performance of the HSYA calibration model needed to be improved.
authors.affiliation: Pharmaceutical Informatics Institute, Zhejiang University, Hangzhou 310058, China.
authors.forename: Shuang-Yue
authors.initials: SY
authors.lastname: Liu
authors.forename: Wen-Long
authors.initials: WL
authors.lastname: Li
authors.forename: Hai-Bin
authors.initials: HB
authors.lastname: Qu
authors.forename: Bu-Chang
authors.initials: BC
authors.lastname: Zhao
authors.forename: Tao
authors.initials: T
authors.lastname: Zhao
mesh_terms: D031187:Carthamus tinctorius / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical / Q000379:methods / Q000592:standards*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification*; D009862:Online Systems; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; C540581:danhong