title: [Effect of curcumin combined with ATRA on differentiation of ATRA-resistant acute promyelocytic leukemia cells].
abstract: In order to investigate the effect of curcumin combined with all-trans retinoid acid (ATRA) on differentiation of ATRA-resistant acute promyelocytic leukemia (APL) cells and its molecular mechanism, the NB4-R1, an ATRA-resistant APL cells, was used as a model, counting of NB4-R1 and cell morphologic observation were performed, the effect of curcumin alone or combined with ATRA on proliferation, differentiation of NB4-R1 cells was detected by flow cytometry (FCM), the change of AKT phosphorylation in cell differentiation was detected by Western blot. The results showed that ATRA had no influence on NB4-R1 cell proliferation, but enhanced the inhibitory effect of curcumin on NB4-R1 cell growth; the curcumin or ATRA alone did not affect NB4-R1 differentiation; curcumin combined with ATRA could obviously induce CD11b expression; the cell morphology showed obvious differentiation characteristics. ATRA could promote phosphorylation of AKT in NB4 cells at short time, but not had effect on phosphorylation of AKT in NB4-R1 cells; the curcumin could enhance the phosphorylation of AKT in NB4-1R cells, the curcumin combined with ATRA could further enhance the phosphorylation of AKT. It is concluded that PI3K/AKT pathway inactivation may be one of the factors of drug resistance in APL and curcumin promotes differentiation of NB4-R1 through activating PI3K/AKT pathway.
authors.affiliation: Clinical Laboratory, the First Affilianted Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China.
authors.forename: Tang-Yong
authors.initials: TY
authors.lastname: Chen
authors.forename: Fen
authors.initials: F
authors.lastname: Xu
authors.forename: Yun-Yuan
authors.initials: YY
authors.lastname: Kong
authors.forename: Fang
authors.initials: F
authors.lastname: Wen
authors.forename: Fu-Yuan
authors.initials: FY
authors.lastname: Xie
authors.forename: La-Gen
authors.initials: LG
authors.lastname: Wan
authors.forename: Zhang-Lin
authors.initials: ZL
authors.lastname: Zhang
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D014212:Tretinoin; D051057:Proto-Oncogene Proteins c-akt; D003474:Curcumin