title: On the potential of mass spectrometry-based metabolite profiling approaches to the study of biochemical adaptation in psychrophilic yeast.
abstract: To move beyond targeted approaches to the biochemical characterization of psychrophilic yeast and provide a more holistic understanding of the chemistry of physiological adaptation of psychrophiles at the molecular level, ultraperformance liquid chromatography combined with simultaneous acquisition of low- and high-collision energy mass spectra (UPLC/MS(e)) was employed for a preliminary comparative analysis of cell extracts of psychrophilic Antarctic yeasts Cryptococcus vishniacii CBS 10616 and Dioszegia cryoxerica CBS 10919 versus the mesophile Saccharomyces cerevisiae 'cry havoc'. A detailed workflow for providing high-confidence preliminary identifications of psychrophilic yeast-specific molecular features is presented. Preliminary identifications of psychrophile-specific features in C. vishniacii and D. cryoxerica determined with the described method include the glycerophospholipids lysophosphatidylcholine 18:2, lysophosphatidylcholine 18:3, lysophosphatidylethanolamine 18:3, and lysophosphatidylethanolamine 18:2. In addition, levels of guanosine diphosphate appear significantly elevated in cell extracts of the psychrophilic yeasts as compared to Saccharomyces cerevisiae. Finally, five psychrophilic yeast-specific peptides have been discovered. All of these are demonstrated to be glycine- and/or proline-rich, a known structural characteristic of many naturally occurring bioactive peptides. The potential of this untargeted metabolite profiling approach as a tool for knowledge discovery and hypothesis generation in the study of biodiversity and microbial adaptation is highlighted.
authors.affiliation: Department of Chemistry, University of Minnesota, Minneapolis, MN, 55455, USA, jdalluge@umn.edu.
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Dalluge
authors.forename: Laurie B
authors.initials: LB
authors.lastname: Connell
mesh_terms: D000222:Adaptation, Physiological; D001487:Basidiomycota / Q000378:metabolism*; D003080:Cold Temperature; D003454:Cryptococcus / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D008246:Lysophospholipids / Q000378:metabolism; D013058:Mass Spectrometry; D055442:Metabolome; D012441:Saccharomyces cerevisiae / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D008246:Lysophospholipids