title: Extraction of antioxidative and antihypertensive bioactive peptides from Parkia speciosa seeds.
abstract: Antioxidative and antihypertensive bioactive peptides were successfully derived from Parkia speciosa seed using alcalase. The effects of temperature (25 and 50 °C), substrate-to-enzyme ratio (S/E ratio, 20 and 50), and incubation time (0.5, 1, 2 and 5h) were evaluated based on 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and angiotensin-converting enzyme (ACE) assays. Bioactive peptide extracted at a hydrolysis condition of: temperature=50 °C, S/E ratio=50 and incubation time=2h, exhibited the highest DPPH radical scavenging activity (2.9 mg GAE/g), reducing power (11.7 mM) and %ACE-inhibitory activity (80.2%). The sample was subsequently subjected to fractionation and the peptide fraction of <10 kDa showed the strongest bioactivities. A total of 29 peptide sequences from peptide fraction of <10 kDa were identified as the most potent contributors to the bioactivities. These novel bioactive peptides were suggested to be beneficial to nutraceutical and food industries.
authors.affiliation: Bioprocess Division, School of Industrial Technology, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.
authors.forename: Hwee-Leng
authors.initials: HL
authors.lastname: Siow
authors.forename: Chee-Yuen
authors.initials: CY
authors.lastname: Gan
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D000959:Antihypertensive Agents / Q000737:chemistry* / Q000302:isolation & purification; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D007887:Fabaceae / Q000737:chemistry*; D006868:Hydrolysis; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D007703:Peptidyl-Dipeptidase A / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D000975:Antioxidants; D010455:Peptides; D010936:Plant Extracts; D007703:Peptidyl-Dipeptidase A
keywords: Antihypertensive; Antioxidative; Bioactive peptide; Parkia speciosa