title: The influence of selenium compounds of different structure on morphology, blood biochemistry and phagocytic capability of granulocytes in rats.
abstract: BACKGROUND
  Selenium belongs to important microelements. Numerous studies have revealed relationships between its deficiency and occurrence of diverse illnesses, but the question of the proper form and dose of Se-supplementation still remains unsolved.
  
  
  OBJECTIVE
  In the present study the influence of different selenium compounds on blood morphology and biochemistry as well as on phagocytic capacity of granulocytes and NBT test in rats was investigated.
  
  
  METHODS
  Adolescent male Wistar rats were divided into four groups (ten animals each): I--control, received saline; II--received sodium selenite Na2SeO3; III--received selenoorganic compound A of chain structure 4-(o-tolyl-)-selenosemicarbazide of 2-chlorobenzoic acid; IV--received selenoorganic compound B of cyclic structure 3-(2-chlorobenzoylamino-)-2-(o-tolylimino-)-4-methyl-4-selenazoline. The administration was performed by stomach tube at a dose of 5 x 10(-4) mg Se g(-1) b.w. once a day for 10 days.
  
  
  RESULTS
  Selenium compounds treatment decreased haematocrit. Erythrocytes number was unchanged in all groups receiving Se vs. control, whereas leucocytes number was depressed in groups II and IV. Haemoglobin was significantly decreased in group III. White blood count was altered in groups II and III, where all parameters were markedly decreased except for lymphocytes in group III and remained unchanged in group IV. The outcomes regarding selenium effect on biochemistry parameters of blood showed that urea remained unchanged, glucose was statistically decreased in groups II and III, whereas cholesterol was significantly diminished in group II and increased in group III vs. control. Results concerning phagocytosis and NBT test displayed that % of positive cells were decreased in groups II and III, whereas remained unaltered in group IV vs. control.
  
  
  CONCLUSIONS
  As cyclic selenoorganic compound B did not cause many significant changes of the studied parameters it may be suggested that after further researches it could be taken into account as a possible selenium supplement.
authors.affiliation: Chair and Department of Medical Chemistry, Medical University in Lublin, Poland. irena.musik@umlub.pl
authors.forename: Irena
authors.initials: I
authors.lastname: Musik
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kiełczykowska
authors.forename: Helena
authors.initials: H
authors.lastname: Donica
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects; D006098:Granulocytes / Q000187:drug effects*; D007962:Leukocytes / Q000187:drug effects; D008297:Male; D009504:Neutrophils / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D017670:Sodium Compounds / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D017670:Sodium Compounds