title: Aberrant septin 9 DNA methylation in colorectal cancer is restricted to a single CpG island.
abstract: BACKGROUND
  The septin 9 gene (SEPT9) codes for a GTP-binding protein associated with filamentous structures and cytoskeleton formation. SEPT9 plays a role in multiple cancers as either an oncogene or a tumor suppressor gene. Regulation of SEPT9 expression is complex and not well understood; however, hypermethylation of the gene was recently introduced as a biomarker for early detection of colorectal cancer (CRC) and has been linked to cancer of the breast and of the head and neck. Because the DNA methylation landscape of different regions of SEPT9 is poorly understood in cancer, we analyzed the methylation patterns of this gene in distinct cell populations from normal and diseased colon mucosa.
  
  
  METHODS
  Laser capture microdissection was performed to obtain homogeneous populations of epithelial and stromal cells from normal, adenomatous, and tumorous colon mucosa. Microdissected samples were analyzed using direct bisulfite sequencing to determine the DNA methylation status of eight regions within and near the SEPT9 gene. Septin-9 protein expression was assessed using immunohistochemistry (IHC).
  
  
  RESULTS
  Regions analyzed in SEPT9 were unmethylated in normal tissue except for a methylation boundary detected downstream of the largest CpG island. In adenoma and tumor tissues, epithelial cells displayed markedly increased DNA methylation levels (>80%, p <0.0001) in only one of the CpG islands investigated. SEPT9 methylation in stromal cells increased in adenomatous and tumor tissues (≤50%, p <0.0001); however, methylation did not increase in stromal cells of normal tissue close to the tumor. IHC data indicated a significant decrease (p <0.01) in Septin-9 protein levels in epithelial cells derived from adenoma and tumor tissues; Septin-9 protein levels in stromal cells were low in all tissues.
  
  
  CONCLUSIONS
  Hypermethylation of SEPT9 in adenoma and CRC specimens is confined to one of several CpG islands of this gene. Tumor-associated aberrant methylation originates in epithelial cells; stromal cells appear to acquire hypermethylation subsequent to epithelial cells, possibly through field effects. The region in SEPT9 with disease-related hypermethylation also contains the CpGs targeted by a novel blood-based screening test (Epi proColon®), providing further support for the clinical relevance of this biomarker.
authors.affiliation: Epigenomics AG, Berlin, Germany. reinhold.wasserkort@gmx.net.
authors.forename: Reinhold
authors.initials: R
authors.lastname: Wasserkort
authors.forename: Alexandra
authors.initials: A
authors.lastname: Kalmar
authors.forename: Gabor
authors.initials: G
authors.lastname: Valcz
authors.forename: Sandor
authors.initials: S
authors.lastname: Spisak
authors.forename: Manuel
authors.initials: M
authors.lastname: Krispin
authors.forename: Kinga
authors.initials: K
authors.lastname: Toth
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Tulassay
authors.forename: Andrew Z
authors.initials: AZ
authors.lastname: Sledziewski
authors.forename: Bela
authors.initials: B
authors.lastname: Molnar
mesh_terms: D000328:Adult; D000368:Aged; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D018899:CpG Islands; D019175:DNA Methylation; D005260:Female; D023061:Gene Order; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D058112:Septins / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult
chemical_list: C474315:SEPTIN9 protein, human; D058112:Septins