title: ScFKBP12 bridges rapamycin and AtTOR in Arabidopsis.
abstract: FKBP12 encodes a prolyl isomerase and highly conserved in eukaryotic species. In yeasts and animals, FKBP12 can interact with rapamycin and FK506 to form rapamycin-FKBP12 and FK506-FKBP12 complex, respectively. In higher plants, FKBP12 protein lost its function to bind rapamycin and FK506. Early studies showed that yeast and human FKBP12 protein can restore the rapamycin sensitivity in Arabidopsis, but the used concentration is 100-1000 folds higher than that in yeast and animals. High concentration of drugs would increase the cost and cause the potential secondary effects on plant growth and development. Here we further discovered that BP12 plants generated in our previous study are hypersensitive to rapamycin at the concentration as low as that is effective in yeast and animals. It is surprising to observe that WT and BP12 plants are not sensitive to FK506 in normal growth condition. These findings advance the current understanding of rapamycin-TOR signaling in plants.
authors.affiliation: Biotechnology Research Institute; Chinese Academy of Agricultural Sciences; Beijing, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Biotechnology Research Institute; Chinese Academy of Agricultural Sciences; Beijing, PR China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Meng
authors.affiliation: Biotechnology Research Institute; Chinese Academy of Agricultural Sciences; Beijing, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Biotechnology Research Institute; Chinese Academy of Agricultural Sciences; Beijing, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Deng
authors.affiliation: School of Life Sciences; Chongqing University; Chongqing, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.affiliation: Biotechnology Research Institute; Chinese Academy of Agricultural Sciences; Beijing, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Biotechnology Research Institute; Chinese Academy of Agricultural Sciences; Beijing, PR China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Sun
authors.affiliation: Biotechnology Research Institute; Chinese Academy of Agricultural Sciences; Beijing, PR China.
authors.forename: Sandui
authors.initials: S
authors.lastname: Guo
authors.affiliation: Biotechnology Research Institute; Chinese Academy of Agricultural Sciences; Beijing, PR China; School of Life Sciences; Chongqing University; Chongqing, PR China.
authors.forename: Maozhi
authors.initials: M
authors.lastname: Ren
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D030821:Plants, Genetically Modified; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D020123:Sirolimus / Q000494:pharmacology*; D022061:Tacrolimus Binding Protein 1A / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D019869:Phosphatidylinositol 3-Kinases; D029701:Saccharomyces cerevisiae Proteins; D020123:Sirolimus; D022061:Tacrolimus Binding Protein 1A; C512171:TOR protein, Arabidopsis
keywords: Arabidopsisgrowth; FK506; rapamycin; yeast FKBP12