title: Multianalyte sensing of addictive over-the-counter (OTC) drugs.
abstract: A supramolecular sensor array composed of two fluorescent cucurbit[n]uril-type receptors (probe 1 and probe 2) displaying complementary selectivities was tested for its ability to detect and quantify drug-related amines. The fluorimetric titration of the individual probes showed highly variable and cross-reactive analyte-dependent changes in fluorescence. An excellent ability to recognize a variety of analytes was demonstrated in qualitative as well as quantitative assays. Importantly, a successful quantitative analysis of several analytes of interest was achieved in mixtures and in human urine. The throughput and sensitivity surpass those of the current state-of-the-art methods that usually require analyte solid-phase extraction (SPE). These results open up the opportunity for new applications of cucurbit[n]uril-type receptors in sensing and pave the way for the development of simple high-throughput assays for various drugs in the near future.
authors.affiliation: Department of Chemistry and Center for Photochemical Sciences, Bowling Green State University , Bowling Green, Ohio 43403, United States.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Minami
authors.forename: Nina A
authors.initials: NA
authors.lastname: Esipenko
authors.forename: Ali
authors.initials: A
authors.lastname: Akdeniz
authors.forename: Ben
authors.initials: B
authors.lastname: Zhang
authors.forename: Lyle
authors.initials: L
authors.lastname: Isaacs
authors.forename: Pavel
authors.initials: P
authors.lastname: Anzenbacher
mesh_terms: D000588:Amines / Q000032:analysis / Q000737:chemistry / Q000652:urine; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D006801:Humans; D007093:Imidazoles / Q000737:chemistry; D008958:Models, Molecular; D008968:Molecular Conformation; D004366:Nonprescription Drugs / Q000032:analysis* / Q000737:chemistry; D019966:Substance-Related Disorders; D013997:Time Factors
chemical_list: D000588:Amines; D007093:Imidazoles; D004366:Nonprescription Drugs; C029899:imidazole