title: Androgen receptor deficiency alters the arginine-vasopressin sexually dimorphic system in Tfm rats.
abstract: In rodents as well as in many other mammalian and non-mammalian species, the arginine-vasopressin (AVP) system includes a parvocellular sexually dimorphic portion located within the bed nucleus of the stria terminalis (BST), the medial amygdaloid nucleus (MeA) and the lateral septum. In this system, males have more cells and denser projections than females, neurons show androgen and estrogen receptors, and gonadal hormones are required for the activation. However, the role of these hormones for the differentiation of the system is not clear. Previous studies performed on aromatase knockout mice suggested that estradiol is not necessary for the differentiation of the system, but it is important for its activation in adulthood. To elucidate the role of androgens on differentiation and functioning of AVP parvocellular system, we compared male and female rats with a non-functional mutation of androgen receptor (Tfm, testicular feminization mutation) to their control littermates. Our data show that the lack of a functional androgen receptor significantly decreases the expression of AVP immunoreactivity within the BST and MeA of male Tfm. Thus supporting the hypothesis that androgens, through the action of their receptor, should have a relevant role in the organization and modulation of the AVP parvocellular sexually dimorphic system.
authors.affiliation: Department of Neuroscience, University of Torino, Torino, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Allieri
authors.forename: G
authors.initials: G
authors.lastname: Spigolon
authors.forename: R C
authors.initials: RC
authors.lastname: Melcangi
authors.forename: P
authors.initials: P
authors.lastname: Collado
authors.forename: A
authors.initials: A
authors.lastname: Guillamón
authors.forename: S
authors.initials: S
authors.lastname: Gotti
authors.forename: G C
authors.initials: GC
authors.lastname: Panzica
mesh_terms: D000704:Analysis of Variance; D013734:Androgen-Insensitivity Syndrome / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D000831:Animals, Newborn; D001127:Arginine Vasopressin / Q000378:metabolism*; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D009154:Mutation / Q000235:genetics; D009474:Neurons / Q000473:pathology; D051381:Rats; D011944:Receptors, Androgen / Q000172:deficiency*; D012727:Sex Characteristics
chemical_list: D011944:Receptors, Androgen; D001127:Arginine Vasopressin
keywords: AFP; AFP-KO; AIS; ANOVA; AR; AVP; AVP-ir; BST; DHT; E(2); ED; ER; LS; MPOA; MeA; MeCP2; PBS; PND; T; Tfm; Tfm rats; analysis of variance; androgen insensitivity syndrome; androgen receptor; arginine vasopressin; arginine-vasopressin; arginine-vasopressin immunoreactive; bed nucleus of the stria terminalis; dihydrotestosterone; embryonic day; estradiol; estrogen receptor; lateral septum; medial amygdaloid nucleus; medial preoptic area; methyl-CpG-binding protein 2; phosphate-buffered saline; postnatal day; testicular feminization mutation; testosterone; α-fetoprotein; α-fetoprotein knockout mice