title: GC-MS/MS method for the quantification of α-cedrene in rat plasma and its pharmacokinetic application.
abstract: α-Cedrene is a pharmacologically active ingredient isolated from the essential oil of cedar. A selective and sensitive GC-MS/MS method was developed for the quantification of α-cedrene in rat plasma for the first time. α-Cedrene was extracted from rat plasma using ethyl acetate at neutral pH. The analytes were determined in selective reaction monitoring mode using MS/MS: m/z 204.3→119.0 for α-cedrene and m/z 146.0→111.0 for 1,4-dichlorobenzene (internal standard). The standard curve was linear (r(2) ≥ 0.995) over the concentration ranges of 5-800 ng/mL. The lower limit of quantification was 5 ng/mL using 50 μL of rat plasma. The coefficient of variation and relative error for intra- and interassays at four quality control levels were 3.1-13.9% and -4.0-2.6%, respectively. The stability of processing (freeze-thaw, long-term storage at -80°C, and short-term storage at room temperature) and chromatography (reinjection) was shown to be of insignificant effect. The present method was applied successfully to the pharmacokinetic study of α-cedrene after its intravenous (10 mg/kg) and oral (25 mg/kg) administration in male Sprague-Dawley rats.
authors.affiliation: Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy, The Catholic University of Korea, Bucheon, Korea; College of Pharmacy, Kyung Hee University, Seoul, Korea.
authors.forename: Joo Yeon
authors.initials: JY
authors.lastname: Hong
authors.forename: Byung Ho
authors.initials: BH
authors.lastname: Lee
authors.forename: Tae Hwan
authors.initials: TH
authors.lastname: Kim
authors.forename: Jongki
authors.initials: J
authors.lastname: Hong
authors.forename: Kyoung Mee
authors.initials: KM
authors.lastname: Lee
authors.forename: Sun Dong
authors.initials: SD
authors.lastname: Yoo
authors.forename: Hye Suk
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002849:Chromatography, Gas; D007275:Injections, Intravenous; D008297:Male; D000081223:Polycyclic Sesquiterpenes; D051381:Rats; D017207:Rats, Sprague-Dawley; D012717:Sesquiterpenes / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C000393:cedrene
keywords: Cedar; Gas chromatography tandem mass spectrometry; Pharmacokinetics; Rat plasma; α-Cedrene