title: An electrochemical DNA biosensor based on gold nanorods decorated graphene oxide sheets for sensing platform.
abstract: A simple electrochemical sensor for sensitive and selective DNA detection was constructed based on gold nanorods (Au NRs) decorated graphene oxide (GO) sheets. The high-quality Au NRs-GO nanocomposite was synthesized via the electrostatic self-assembly technique, which is considered a potential sensing platform. Differential pulse voltammetry was used to monitor the DNA hybridization event using methylene blue as an electrochemical indicator. Under optimal conditions, the peak currents of methylene blue were linear with the logarithm of the concentrations of complementary DNA from 1.0 × 10(-9) to 1.0 × 10(-14)M with a detection limit of 3.5 × 10(-15)M (signal/noise=3). Moreover, the prepared electrochemical sensor can effectively distinguish complementary DNA sequences in the presence of a large amount of single-base mismatched DNA (1000:1), indicating that the biosensor has high selectivity.
authors.affiliation: College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, People's Republic of China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Han
authors.forename: Xian
authors.initials: X
authors.lastname: Fang
authors.forename: Anqi
authors.initials: A
authors.lastname: Shi
authors.forename: Jiao
authors.initials: J
authors.lastname: Wang
authors.forename: Yuzhong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000032:analysis*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization; D010087:Oxides / Q000737:chemistry
chemical_list: D010087:Oxides; D006046:Gold; D006108:Graphite; D004247:DNA
keywords: DNA biosensor; Differential pulse voltammetry; Gold nanorods; Graphene oxide