title: Effect of cerium oxide nanoparticles on intestinal serotonin in zebrafish.
abstract: Cerium oxide nanoparticles or nanoceria are emerging as a new and promising class of nanoparticle technology for biomedical applications. The safe implementation of these particles in clinical applications requires evaluation of their redox properties and reactivity that might cause neurotoxic effects by interacting with redox components of the physiological environment. We reportin vitroandin vivostudies to evaluate the impact of nanoceria exposure on serotonin (5-HT), an important neurotransmitter that plays a critical role in various physiological processes including motility and secretion in the digestive system.In vitrostudies of 5-HT in the presence of nanoceria using spectroscopic, electrochemical and surface characterization methods demonstrate that nanoceria interacts with 5-HT and forms a surface adsorbed 5-HT-nanoceria complex. Furtherin vivostudies in live zebrafish embryos indicate depletion of the 5-HT level in the intestine for exposure periods longer than three days. Intestinal 5-HT was assessed quantitatively in live embryos using implantable carbon fiber microelectrodes and the results were compared to immunohistochemistry of the dissected intestine. 20 and 50 ppm nanoparticle exposure decreased the 5-HT level to 20.5 (±1.3) and 5.3 (±1.5) nM respectively as compared to 30.8 (±3.4) nM for unexposed control embryos. The results suggest that internalized nanoceria particles can concentrate 5-HT at the nanoparticle accumulation site depleting it from the surrounding tissue. This finding might have long term implications in the neurophysiology and functional development of organisms exposed to these particles through intended or unintended exposure.
authors.affiliation: Department of Chemistry and Biomolecular Science, Clarkson University Potsdam, NY 13699-5810, USA.
authors.forename: Rıfat Emrah
authors.initials: RE
authors.lastname: Ozel
authors.forename: Akhtar
authors.initials: A
authors.lastname: Hayat
authors.forename: Kenneth N
authors.initials: KN
authors.lastname: Wallace
authors.forename: Silvana
authors.initials: S
authors.lastname: Andreescu
keywords: implantable microelectrodes; intestine; nanoceria; nanotoxicity; neurotoxicity; serotonin; zebrafish embryos