title: Polymer-based alternative method to extract bromelain from pineapple peel waste.
abstract: Bromelain is a mixture of proteolytic enzymes present in all tissues of the pineapple (Ananas comosus Merr.), and it is known for its clinical therapeutic applications, food processing, and as a dietary supplement. The use of pineapple waste for bromelain extraction is interesting from both an environmental and a commercial point of view, because the protease has relevant clinical potential. We aimed to study the optimization of bromelain extraction from pineapple waste, using the aqueous two-phase system formed by polyethylene glycol (PEG) and poly(acrylic acid). In this work, bromelain partitioned preferentially to the top/PEG-rich phase and, in the best condition, achieved a yield of 335.27% with a purification factor of 25.78. The statistical analysis showed that all variables analyzed were significant to the process.
authors.affiliation: Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Science, University of São Paulo, São Paulo, Brazil.
authors.forename: Letícia Celia de Lencastre
authors.initials: LC
authors.lastname: Novaes
authors.forename: Valéria de Carvalho Santos
authors.initials: Vde C
authors.lastname: Ebinuma
authors.forename: Priscila Gava
authors.initials: PG
authors.lastname: Mazzola
authors.forename: Adalberto
authors.initials: A
authors.lastname: Pessoa
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D031230:Ananas / Q000201:enzymology*; D001963:Bromelains / Q000737:chemistry / Q000302:isolation & purification*; D007220:Industrial Waste; D011092:Polyethylene Glycols / Q000737:chemistry*; D012037:Refuse Disposal
chemical_list: D000180:Acrylic Resins; D007220:Industrial Waste; D011092:Polyethylene Glycols; C006903:carbopol 940; D001963:Bromelains
keywords: aqueous two-phase system; bromelain; pineapple waste; poly(acrylic acid); polyethylene glycol