title: Determination of biflavonoids in four Mexican species of Selaginella by HPLC.
abstract: The genus Selaginella comprises several species, some of which grow on the American continent. Among these species, S. nothohybrida Valdespino, S. lepidophylla Hook et Grev, S. pallescens (Presl) Spring, and S. reflexa Underw. are found in several states of the Mexican Republic. The aforementioned three species are used medicinally, typically to treat renal disorders. This paper describes the development of an HPLC-UV method for the determination of amentoflavone (1), robustaflavone (2), and (2S)-2,3-dihydrorobustaflavone (3) as major components of the above-mentioned species of Selaginella. Components 1, 2, and 3 were quantitatively determined using an XBridge Waters C18 5 microm column, with a gradient system consisting of mixtures of acetonitrile and water with 0.4% acetic acid. The flow rate was 0.4 mL/min, with UV detection at 367 nm. LOD and LOQ values were in the range of 0.025 to 0.216 microg/mL. Compounds 1, 2, and 3 showed good linearity in the 1.2 to 18 microg/mL range; recovery was within 98.2 and 101.9% for all three cases. Compounds 1 and 2 were detected in all eight samples; their concentration ranged from 0.35 to 1.79 mg/g of plant. Thus, compounds 1 and 2 could be used as markers for S. nothohybrida, S. lepidophylla, S. pallescens, and S. reflexa. In addition, trehalose was detected in all samples as two peaks at 1.5 and 2.0 min. The HPLC method described here was shown to be reliable, reproducible, and accurate and can be used for QC of Selaginella medicinal materials.
authors.affiliation: Universidad Nacional Autónoma de Mexico, Facultad de Química, Departamento de Farmacia, Ciudad Universitaria, Coyoacán 04510, México D.F., México.
authors.forename: María Isabel
authors.initials: MI
authors.lastname: Aguilar
authors.forename: Itzel A
authors.initials: IA
authors.lastname: Mejía
authors.forename: César
authors.initials: C
authors.lastname: Menchaca
authors.forename: Iraís
authors.initials: I
authors.lastname: Vázquez
authors.forename: Andrés
authors.initials: A
authors.lastname: Navarrete
authors.forename: María Isabel
authors.initials: MI
authors.lastname: Chávez
authors.forename: Alberto
authors.initials: A
authors.lastname: Reyes-García
authors.forename: Ramiro
authors.initials: R
authors.lastname: Rios-Gómez
mesh_terms: D044946:Biflavonoids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015203:Reproducibility of Results; D032503:Selaginellaceae / Q000737:chemistry*
chemical_list: D044946:Biflavonoids