title: Acetylcholinesterase activity in the cerebrospinal fluid of dogs with seizures.
abstract: Recent studies in animal models have focused on the role of cholinergic elements, mainly acetylcholinesterase (AChE) and the 'readthrough' acetylcholinesterase isoform (AChE-R), in seizures. A prospective double-masked study was conducted to assess the activity of AChE and AChE-R in cerebrospinal fluid (CSF) of 26 dogs post-seizure, 28 dogs with intervertebral disc disease (IVDD) and 16 healthy dogs. AChE was also measured in the serum in the post-seizure and IVDD groups. The results showed no significant differences in CSF AChE among the three groups. AChE-R was not detected in any dog and AChE in the serum was similar between groups. This preliminary study provides new information on AChE and AChE-R in the CSF and sera of dogs following naturally-occurring seizures.
authors.affiliation: Koret School of Veterinary Medicine, Hebrew University of Jerusalem, Rehovot 76100, Israel. Electronic address: oritchai@yahoo.com.
authors.forename: Orit
authors.initials: O
authors.lastname: Chai
authors.forename: Adi
authors.initials: A
authors.lastname: Sommer
authors.forename: Gabriel
authors.initials: G
authors.lastname: Zimmerman
authors.forename: Hermona
authors.initials: H
authors.lastname: Soreq
authors.forename: Alon
authors.initials: A
authors.lastname: Friedman
authors.forename: Tali
authors.initials: T
authors.lastname: Bdolah-Abram
authors.forename: Itamar
authors.initials: I
authors.lastname: Aroch
authors.forename: Merav H
authors.initials: MH
authors.lastname: Shamir
mesh_terms: D000110:Acetylcholinesterase / Q000097:blood* / Q000134:cerebrospinal fluid*; D000818:Animals; D003124:Colorimetry / Q000662:veterinary; D004228:Dithionitrobenzoic Acid / Q000378:metabolism; D004283:Dog Diseases / Q000097:blood / Q000201:enzymology* / Q000503:physiopathology; D004285:Dogs; D004311:Double-Blind Method; D004586:Electrophoresis / Q000662:veterinary; D005260:Female; D007557:Israel; D008297:Male; D011446:Prospective Studies; D012640:Seizures / Q000097:blood / Q000201:enzymology / Q000503:physiopathology / Q000662:veterinary*; D018709:Statistics, Nonparametric
chemical_list: D004228:Dithionitrobenzoic Acid; D000110:Acetylcholinesterase
keywords: Acetylcholinesterase; Cerebrospinal fluid; Dog; Epilepsy; Readthrough acetylcholinesterase