title: Liquid chromatography-tandem mass spectrometry method as the golden standard for therapeutic drug monitoring in renal transplant.
abstract: Kidney transplanted patients need immunosuppressant therapies. Therapeutic drug monitoring approaches produce the optimal clinical outcome is still under debate. This review details strength and limits of methods available: immunoassay and chromatography-based. Liquid chromatography with mass spectrometry detection is a major breakthrough in therapeutic drug monitoring of immunosuppressive agents and is considered as the method of choice in therapeutic drug monitoring (TDM) of immunosuppressants. Despite the initial high cost for the instrumentation, HPLC-MS is more cost effective than microparticle enzyme immunoassay. The main important features of LC-MS/MS methodology for immunosuppressive drugs are the shortened analysis time, an increased throughput, higher selectivity, specificity, and sensitivity, and low cost of analysis.
authors.affiliation: Nephrology and Dialysis Department, I.R.C.C.S. "Casa Sollievo della Sofferenza", S. Giovanni Rotondo (Foggia), Italy.
authors.forename: Filippo
authors.initials: F
authors.lastname: Aucella
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Lauriola
authors.forename: Gennaro
authors.initials: G
authors.lastname: Vecchione
authors.forename: Giovanni Luca
authors.initials: GL
authors.lastname: Tiscia
authors.forename: Elvira
authors.initials: E
authors.lastname: Grandone
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods / Q000592:standards; D016903:Drug Monitoring / Q000379:methods / Q000592:standards*; D006801:Humans; D007166:Immunosuppressive Agents / Q000009:adverse effects*; D016030:Kidney Transplantation / Q000009:adverse effects / Q000592:standards*; D053719:Tandem Mass Spectrometry / Q000379:methods / Q000592:standards*
chemical_list: D007166:Immunosuppressive Agents
keywords: Immunosuppression; Kidney transplantation; Liquid chromatography; Mass spectrometry; Therapeutic drug monitoring