title: [Bone marrow-derived dendritic cells rather than spleen-derived dendritic cells can generate regulatory T cells].
abstract: There are many evidences that dendritic cells (DC) can establish and maintain immunological tolerance through inducing the differentiation of regulatory T cells Treg. This study was purposed to explore the possibility to gene-rate Treg from bone marrow-derived DC (BM-DC) or spleen-derived DC (spDC) generated CD4(+) CD25(+) FOXP3(+) Treg by induction. Bone marrow immature DC (imDC) induced from bone marrow precursor cells of C57BL/6 mice by GM-CSF and IL-4; after culture for 6 day, imDC were stimulated by LPS for additional 16 hours and the mature DC (mDC) have been got; the spDC were collected from spleen of C57BL/6 mice by MACS. Co-culturing fresh BALB/c mouse CD4(+) T cells with these three sorts of DC above mentioned respectively was performed to generate CD4(+) CD25(+) FOXP3(+) Treg. The expression of FOXP3 in CD4(+) T cells was detected by flow cytometry, and the capacity of different DC generated CD4(+) CD25(+) FOXP3(+) Treg was evaluated. The results showed that stimulated by C57BL/6 immature or mature DC, the positive rate of FOXP3 in BALB/c CD4(+) T cells increased from (8.57 ± 1.14)% to (15.80 ± 1.35)%, (17.93 ± 1.45)% respectively (P < 0.01); while stimulated by spDC, the positive rate of FOXP3 in BALB/c CD4(+) T cells decreased from (8.57 ± 1.14)% to (3.95 ± 0.79)% (P < 0.05). It is concluded that the BM-DC but not spDC can generate Treg from CD4(+) T cells, BM-DC may mediate immune tolerance rather than the immune response.
authors.affiliation: Medical Blood Laboratory, Department of Laboratory Medicine, Guangdong Provincial Hospital of Traditional Chinese Medical, Guangzhou 510120, Guangdong Province, China.
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Zhou
authors.forename: Xian-Zhang
authors.initials: XZ
authors.lastname: Huang
authors.forename: Xin-Zhong
authors.initials: XZ
authors.lastname: Wu
authors.forename: Wei-Ye
authors.initials: WY
authors.lastname: Chen
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Wu
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology*; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000166:cytology*
chemical_list: D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse