title: Development and characterization of lutein-loaded SNEDDS for enhanced absorption in Caco-2 cells.
abstract: A self-nanoemulsifying drug delivery system (SNEDDS) has been developed for enhanced oral bioavailability of lutein. Its permeation enhancement has been evaluated using monolayers of Caco-2 cells. SNEDDS is composed of a mixture of Lexol® and Emulmetik® 900, Labrasol®, and Tween 80 as oil, surfactant and co-surfactant, respectively. Upon dilution of lutein-loaded SNEDDS with water, a nanoemulsion was obtained in <10 s with spherical droplets of 40-150 nm in diameter. The zeta potential was in the range of -19 to -32 mV. Increasing the ratio of surfactant to co-surfactant decreased the mean droplet size. Dissolution studies showed that lutein was released rapidly (<5 min) from SNEDDS into 0.1 N HCl and pH 6.8 phosphate buffer solution without any aggregation. In vitro studies using Caco-2 cells revealed that lutein-loaded SNEDDS showed shorter lag time and greater (2-fold) cellular accumulation compared with the lutein dispersion.
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Naresuan University , Phitsanulok 65000 , Thailand.
authors.forename: Pattravee
authors.initials: P
authors.lastname: Niamprem
authors.forename: Soravoot
authors.initials: S
authors.lastname: Rujivipat
authors.forename: Waree
authors.initials: W
authors.lastname: Tiyaboonchai
mesh_terms: D000042:Absorption; D001682:Biological Availability; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods; D045703:Emulsifying Agents / Q000737:chemistry*; D004655:Emulsions / Q000737:chemistry; D005989:Glycerides; D006801:Humans; D006863:Hydrogen-Ion Concentration; D014975:Lutein / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D009821:Oils / Q000737:chemistry; D009930:Organic Chemicals / Q000737:chemistry; D010316:Particle Size; D011136:Polysorbates / Q000737:chemistry; D012995:Solubility; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D045703:Emulsifying Agents; D004655:Emulsions; D005989:Glycerides; D009821:Oils; D009930:Organic Chemicals; D011136:Polysorbates; D013501:Surface-Active Agents; C440220:Labrasol; D014975:Lutein