title: Synthesis and characterization of N-(2-aminoethyl)-2-acetamidyl gellan gum with potential biomedical applications.
abstract: N-(2-aminoethyl)-2-acetamidyl gellan gum (GCM-EDA) was prepared by carboxymethylation (via nucleophilic substitution of primary hydroxyl groups of the β-D-glucose unit of gellan gum, in the presence of alkali and chloroacetic acid) and reaction with tert-butyl N-(2-aminoethyl) carbamate (N-Boc-EDA) using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) as an activator, followed by deprotection with trifluoroacetic acid. The structural confirmation and characterization of N-(2-aminoethyl)-2-acetamidyl gellan gum was performed by spectroscopic, rheological and thermogravimetric analysis, and in vitro tests showed a lack of cytotoxicity which is indicative of the potential of this material to be used in biomedical applications.
authors.affiliation: Gh. Asachi Technical University, Faculty of Chemical Engineering and Environmental Protection, Physical-Chemistry Department, 73 A, Prof. Dr. D. Mangeron Street, 700050 Iasi, Romania.
authors.forename: O
authors.initials: O
authors.lastname: Novac
authors.forename: G
authors.initials: G
authors.lastname: Lisa
authors.forename: E
authors.initials: E
authors.lastname: Barbu
authors.forename: F
authors.initials: F
authors.lastname: Alhaique
authors.forename: M I
authors.initials: MI
authors.lastname: Popa
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D060326:Chemistry Techniques, Synthetic; D011135:Polysaccharides, Bacterial / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity; D051381:Rats
chemical_list: D001672:Biocompatible Materials; D011135:Polysaccharides, Bacterial; C048288:gellan gum
keywords: Aminated derivative; Carboxymethylation; Cytotoxicity; Gellan gum; N-Boc deprotection; Rheology