title: Characterization of a methyltransferase involved in herboxidiene biosynthesis.
abstract: The herboxidiene biosynthetic gene cluster contains a regulatory gene and six biosynthetic genes that encode three polyketide synthases (HerB, HerC and HerD) and three tailoring enzymes (HerE, HerF and HerG). Through single crossover recombination, an integrative plasmid was inserted into the genome of Streptomyces chromofuscus ATCC 49982 between herE and herF, resulting in low-level expression of herF and the downstream herG. The mutant strain produced two new compounds, 18-deoxy-25-demethyl-herboxidiene and 25-demethyl-herboxidiene. HerF was expressed in Escherichia coli and biochemically characterized as the dedicated methyltransferase in herboxidiene biosynthesis. It prefers 25-demethyl-herboxidiene to 18-deoxy-25-demethyl-herboxidiene, suggesting that C-25 methylation is the last tailoring step.
authors.affiliation: Department of Applied Chemistry and Biological Engineering, College of Chemical Engineering, Northeast Dianli University, Jilin, Jilin 132012, China; Department of Biological Engineering, Utah State University, 4105 Old Main Hill, Logan, UT 84322, United States.
authors.forename: Dayu
authors.initials: D
authors.lastname: Yu
authors.forename: Fuchao
authors.initials: F
authors.lastname: Xu
authors.forename: Shuwei
authors.initials: S
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Shao
authors.forename: Siyuan
authors.initials: S
authors.lastname: Wang
authors.forename: Jixun
authors.initials: J
authors.lastname: Zhan
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D005233:Fatty Alcohols / Q000737:chemistry / Q000378:metabolism*; D016680:Genome, Bacterial; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D008968:Molecular Conformation; D005810:Multigene Family; D009154:Mutation; D048630:Polyketide Synthases / Q000235:genetics / Q000378:metabolism; D011714:Pyrans / Q000737:chemistry / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D005233:Fatty Alcohols; D011714:Pyrans; C076087:herboxidiene; D048630:Polyketide Synthases; D008780:Methyltransferases
keywords: Anti-cholesterol; Herboxidiene; Methyltransferase; Polyketide biosynthesis; Tailoring reaction