title: Exposure of maternal mice to cis-bifenthrin enantioselectively disrupts the transcription of genes related to testosterone synthesis in male offspring.
abstract: The commercial bifenthrin (BF) contains two cis isomers. In the present study, a dose of 15mg/kg of 1R-cis-BF or 1S-cis-BF was orally administered for 3 weeks to female mice before or during pregnancy. Then, the expression of steroidogenesis related genes which were considered as effective biomarkers of endocrine disruption were analyzed in the male offspring. Maternal exposure to 1S-cis-BF during pregnancy significantly reduced the mRNA levels of peripheral benzodiazepine receptor (PBR) and steroidogenic acute regulatory protein (StAR) in the testes of 3- or 6-week old male offspring. In addition, a significant decrease of cytochrome P450 17α-hydroxysteroid dehydrogenase (P450-17α) was also observed in the testes of 6-week old male offspring when dams were treated with 1S-cis-BF during pregnancy but not before pregnancy. Moreover, the scavenger receptor class B type 1 (SRB1) and cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc) decreased significantly in the testes of 6-week old male offspring when dams were treated with 1S-cis-BF during and before pregnancy. Thus, oral administration of the maternal mice to cis-BF for 3 weeks, particularly during pregnancy, resulted in endocrine disruption in the male offspring, with the 1S-cis-BF causing more significant alterations than the 1R-cis-BF form.
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Yuanxiang
authors.initials: Y
authors.lastname: Jin
authors.forename: Jiangcong
authors.initials: J
authors.lastname: Wang
authors.forename: Xueqing
authors.initials: X
authors.lastname: Sun
authors.forename: Yang
authors.initials: Y
authors.lastname: Ye
authors.forename: Minjie
authors.initials: M
authors.lastname: Xu
authors.forename: Jianai
authors.initials: J
authors.lastname: Wang
authors.forename: Shaoping
authors.initials: S
authors.lastname: Chen
authors.forename: Zhengwei
authors.initials: Z
authors.lastname: Fu
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics; D052244:Endocrine Disruptors / Q000633:toxicity*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007306:Insecticides / Q000737:chemistry / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008431:Maternal-Fetal Exchange; D051379:Mice; D008813:Mice, Inbred ICR; D010750:Phosphoproteins / Q000235:genetics; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D011722:Pyrethrins / Q000737:chemistry / Q000633:toxicity*; D011973:Receptors, LDL / Q000235:genetics; D012098:Reproduction / Q000187:drug effects; D051122:Scavenger Receptors, Class B / Q000235:genetics; D013237:Stereoisomerism; D013737:Testis / Q000187:drug effects / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism*; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D052244:Endocrine Disruptors; D007306:Insecticides; D010750:Phosphoproteins; D011722:Pyrethrins; D011973:Receptors, LDL; C454739:Scarb1 protein, mouse; D051122:Scavenger Receptors, Class B; D000098682:Steroidogenic Acute Regulatory Protein; D013739:Testosterone; C099952:bifenthrin; D002784:Cholesterol; D002786:Cholesterol Side-Chain Cleavage Enzyme
keywords: Enantioselectivity; Gene expression; Male offspring; Reproductive toxicity; cis-Bifenthrin