title: Efficient direct oxygen reduction by laccases attached and oriented on pyrene-functionalized polypyrrole/carbon nanotube electrodes.
abstract: We report the functionalization of multi-walled carbon nanotube (MWCNT) electrodes by oxidative electropolymerization of pyrrole monomers bearing pyrene and N-hydroxysuccinimide groups. Both polymers were applied to the immobilization and electrical wiring of Trametes versicolor laccase via chemical grafting or non-covalent binding. A "pseudo" host-guest interaction of polymerized pyrene with a hydrophobic cavity of laccase was used for the oriented enzyme immobilization on MWCNT electrodes. The latter leads to higher catalytic current for oxygen reduction (1.85 mA cm(-2)) and higher electroenzymatic stability (50% after one month).
authors.affiliation: UJF-Grenoble 1, CNRS, Département de Chimie Moléculaire, UMR-5250, ICMG FR-2607, BP-53, 38041 Grenoble Cedex 9, France. serge.cosnier@ujf-grenoble.fr.
authors.forename: Noémie
authors.initials: N
authors.lastname: Lalaoui
authors.forename: Kamal
authors.initials: K
authors.lastname: Elouarzaki
authors.forename: Alan
authors.initials: A
authors.lastname: Le Goff
authors.forename: Michael
authors.initials: M
authors.lastname: Holzinger
authors.forename: Serge
authors.initials: S
authors.lastname: Cosnier
mesh_terms: D055162:Biocatalysis; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D042845:Laccase / Q000737:chemistry / Q000378:metabolism*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D055454:Trametes / Q000201:enzymology
chemical_list: D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D011108:Polymers; D011721:Pyrenes; D011758:Pyrroles; C067635:polypyrrole; C030984:pyrene; D042845:Laccase; D010100:Oxygen