title: Plasma functionalization of poly(vinyl alcohol) hydrogel for cell adhesion enhancement.
abstract: Tailoring the interface interactions between a biomaterial and the surrounding tissue is a capital aspect to consider for the design of medical devices. Poly(vinyl alcohol) (PVA) hydrogels present suitable mechanical properties for various biological substitutes, however the lack of cell adhesion on their surface is often a problem. The common approach is to incorporate biomolecules, either by blending or coupling. But these modifications disrupt PVA intra- and intermolecular interactions leading therefore to a loss of its original mechanical properties. In this work, surface modification by glow discharge plasma, technique known to modify only the surface without altering the bulk properties, has been investigated to promote cell attachment on PVA substrates. N2/H2 microwave plasma treatment has been performed, and the chemical composition of PVA surface has been investigated. X-ray photoelectron and Fourier transform infrared analyses on the plasma-treated films revealed the presence of carbonyl and nitrogen species, including amine and amide groups, while the main structure of PVA was unchanged. Plasma modification induced an increase in the PVA surface wettability with no significant change in surface roughness. In contrast to untreated PVA, plasma-modified films allowed successful culture of mouse fibroblasts and human endothelial cells. These results evidenced that the grafting was stable after rehydration and that it displayed cell adhesive properties. Thus plasma amination of PVA is a promising approach to improve cell behavior on contact with synthetic hydrogels for tissue engineering.
authors.affiliation: Inserm, U698; Cardiovascular Bio-Engineering; X. BichatHospital; Paris, France; Institut Galilée; University Paris 13; Villetaneuse, France.
authors.forename: Julia M
authors.initials: JM
authors.lastname: Ino
authors.affiliation: Laboratory for Biomaterials and Bioengineering; Department of Materials Engineering & University Hospital Research Center; Laval University; Quebec City, QC Canada.
authors.forename: Pascale
authors.initials: P
authors.lastname: Chevallier
authors.affiliation: Inserm, U698; Cardiovascular Bio-Engineering; X. BichatHospital; Paris, France; Institut Galilée; University Paris 13; Villetaneuse, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Letourneur
authors.affiliation: Laboratory for Biomaterials and Bioengineering; Department of Materials Engineering & University Hospital Research Center; Laval University; Quebec City, QC Canada.
authors.forename: Diego
authors.initials: D
authors.lastname: Mantovani
authors.affiliation: Inserm, U698; Cardiovascular Bio-Engineering; X. BichatHospital; Paris, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Le Visage
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis*; D002448:Cell Adhesion / Q000502:physiology*; D002460:Cell Line; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D058626:Plasma Gases / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D058626:Plasma Gases; D011142:Polyvinyl Alcohol
keywords: amine grafting; hydrogel; nitrogen plasma; poly(vinyl alcohol); surface analysis