title: A new manual retinal thickness measurement protocol to evaluate high myopia patients.
abstract: OBJECTIVE
  To validate a manual measurement protocol for quantifying retinal thickness (RT) using an optical coherence tomography (OCT) device in pathologic myopia patients.
  
  
  METHODS
  The macular Cross Hair protocol of Stratus OCT3 (Carl Zeiss Meditec, Inc., Dublin, Calif., USA) was applied and manual RT gauging was performed using the caliper tool. Foveal and paramacular RT, located at 1 and 2 mm distances from the fovea in both vertical and horizontal scans, were measured. Three consecutive RT measurements were taken to assess measurement reliability. The within-subject coefficient of variation (CVw) and intraclass correlation coefficients (ICC) were calculated to validate the manual method.
  
  
  RESULTS
  The mean axial length of the 29 eyes assessed was 28.28 ± 2.72 mm and the mean spherical refraction was -13.61 ± 6.68 diopters. CVw ranged from 0.86 to 8.73% and ICC varied from 0.81 to 0.98.
  
  
  CONCLUSIONS
  A manual RT measurement protocol could reliably be used in the daily clinic for assessing pathologic myopic patients when OCT software segmentation fails.
authors.affiliation: IOBA, Universidad de Valladolid, Valladolid, Spain.
authors.forename: José Alberto
authors.initials: JA
authors.lastname: de-Lázaro-Yagüe
authors.forename: Alberto
authors.initials: A
authors.lastname: López-Miguel
authors.forename: María Rosa
authors.initials: MR
authors.lastname: Sanabria
authors.forename: Itziar
authors.initials: I
authors.lastname: Fernández-Martínez
authors.forename: Rosa María
authors.initials: RM
authors.lastname: Coco-Martín
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D058452:Axial Length, Eye / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D047728:Myopia, Degenerative / Q000175:diagnosis*; D009929:Organ Size; D012029:Refraction, Ocular; D015203:Reproducibility of Results; D012160:Retina / Q000473:pathology*; D041623:Tomography, Optical Coherence / Q000379:methods; D055815:Young Adult