title: Zhihengliuella flava sp. nov., an actinobacterium isolated from sea sediment, and emended description of the genus Zhihengliuella.
abstract: A novel Gram-staining-positive actinobacterium, designated H85-3(T), was isolated from a sea sediment sample and its taxonomic position was investigated by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain H85-3(T) was closely related to the members of the genus Zhihengliuella with pairwise sequence similarities of 97.4-98.6 %. The peptidoglycan of strain H85-3(T) was found to be of the A4α type with lysine as the diagnostic diamino acid. The menaquinones were MK-9, MK-10 and MK-8 (56 : 30 : 14) and the major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0. These data supported the affiliation of strain H85-3(T) to the genus Zhihengliuella. Meanwhile, the results of DNA-DNA hybridization, along with the differences in some phenotypic characteristics, indicated that strain H85-3(T) should be distinguished from the recognized species of the genus Zhihengliuella. Therefore, strain H85-3(T) represents a novel species of the genus Zhihengliuella, for which the name Zhihengliuella flava sp. nov. is proposed; the type strain is H85-3(T) ( = NBRC 109021(T) = DSM 26152(T)). An emended description of the genus Zhihengliuella is also proposed.
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC) 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Moriyuki
authors.initials: M
authors.lastname: Hamada
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC) 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Chiyo
authors.initials: C
authors.lastname: Shibata
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC) 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Tamura
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC) 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Ken-Ichiro
authors.initials: KI
authors.lastname: Suzuki
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D007564:Japan; D008835:Micrococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2