title: Terriglobus aquaticus sp. nov., isolated from an artificial reservoir.
abstract: A pink-pigmented, chemo-organotrophic bacterium, designated strain 03SUJ4(T), was isolated from the freshwater of Juam reservoir, Republic of Korea (35° 03' 43'' N 127° 14' 15'' E). Cells were aerobic, Gram-reaction-negative and non-motile rods. Strain 03SUJ4(T) grew at pH 6-7 (optimum, pH 6) and at 15-30 °C (optimum, 25 °C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the genus Terriglobus, showing sequence similarities of 97.09 % and 96.82 % to Terriglobus roseus DSM 18391(T) and Terriglobus saanensis SP1PR4(T), respectively. Low rpoB gene sequence similarity with members of the genus Terriglobus and different fingerprints with the repetitive primers BOX, ERIC and REP indicated that the isolate represented a novel species of the genus Terriglobus. The major cellular fatty acids were iso-C15 : 0, C16 : 0, C20 : 1ω9c, C14 : 0 and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c). The DNA G+C content of strain 03SUJ4(T) was 63.2±0.1 mol% (mean±sd of three determinations). The predominant menaquinone was MK-8. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and two unidentified phospholipids. Several phenotypic characteristics served to differentiate the novel isolate from recognized members of the genus Terriglobus. On the basis of the evidence presented in this study, a novel species, Terriglobus aquaticus sp. nov. is proposed for strain 03SUJ4(T) ( = KCTC 23332(T) = JCM 17517(T)).
authors.affiliation: Department of Biological Sciences, Korea Basic Science Institute, Daejeon 305-806, Republic of Korea.
authors.forename: Keun Sik
authors.initials: KS
authors.lastname: Baik
authors.affiliation: Department of Biological Sciences, Korea Basic Science Institute, Daejeon 305-806, Republic of Korea.
authors.forename: Jong-Soon
authors.initials: JS
authors.lastname: Choi
authors.affiliation: Gwangju Center, Korea Basic Science Institute, Gwangju 500-757, Republic of Korea.
authors.forename: Joseph
authors.initials: J
authors.lastname: Kwon
authors.affiliation: Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors.forename: Seong Chan
authors.initials: SC
authors.lastname: Park
authors.affiliation: Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors.forename: Yeoung Min
authors.initials: YM
authors.lastname: Hwang
authors.affiliation: Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors.forename: Mi Sun
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Department of Dental Hygiene, Gwangju Health College, Gwangju 506-701, Republic of Korea.
authors.forename: Eun Mi
authors.initials: EM
authors.lastname: Kim
authors.affiliation: Department of Bio-Environmental Sciences, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors.forename: Dong-Cheol
authors.initials: DC
authors.lastname: Seo
authors.affiliation: Department of Bio-Environmental Sciences, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors.forename: Ju-Sik
authors.initials: JS
authors.lastname: Cho
authors.affiliation: Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors.forename: Chi Nam
authors.initials: CN
authors.lastname: Seong
mesh_terms: D061271:Acidobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005618:Fresh Water / Q000382:microbiology*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8