title: Gene expression in the vascular wall of the aortic arch in spontaneously hypertensive hyperlipidemic model rats using DNA microarray analysis.
abstract: OBJECTIVE
  In recent years, there has been an increase in patients with arteriosclerosis and the risk of lifestyle-related diseases. However, the pathogenesis and medication of atherosclerosis have not been elucidated. We developed a rat model of lifestyle-related diseases by feeding a high-fat diet and 30% sucrose solution (HFDS) to spontaneously hypertensive hyperlipidemic rats (SHHR) and reported that this model is a useful model of early atherosclerosis. In order to elucidate the pathogenesis of early atherosclerosis, we searched for atherosclerosis-related genes by microarray analysis using the aortic arch rat model of lifestyle-related diseases.
  
  
  METHODS
  Four-month-old male Sprague-Dawley rats and SHHR were each divided into two normal diet (ND) groups and two HFDS groups. After a four-month treatment, the expression of mRNA in the aortic arch was detected using the oligo DNA microarray one-color method and quantified using real-time PCR.
  
  
  RESULTS
  In this study, we detected 39 genes in microarray analysis. Esm1, Retnlb Mkks, and Grem2 showed particularly marked changes in gene expression in the SHHR-HFDS group. Compared with the SD-ND group, the SHHR-HFDS group had an increase in Mkks gene expression of about 26-fold and an approximately 22-fold increase in the expression of Grem2. Similarly, the expression of Esm1 increased by about 12-fold and that of Retnlg by about 10-fold as shown by quantitative real-time PCR.
  
  
  CONCLUSIONS
  This study suggested that these four genes might be important in early atherosclerosis development.
authors.affiliation: Department of Pharmacology, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan; Department of Pharmacogenomics, St. Marianna University Graduate School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa 216-8511, Japan; Division of Endocrinology and Metabolism, Department of Internal Medicine, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, Yokohama, Kanagawa 227-8501, Japan. Electronic address: unfiny@gmail.com.
authors.forename: Go
authors.initials: G
authors.lastname: Koizumi
authors.forename: Toshio
authors.initials: T
authors.lastname: Kumai
authors.forename: Shunya
authors.initials: S
authors.lastname: Egawa
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yatomi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hayashi
authors.forename: Go
authors.initials: G
authors.lastname: Oda
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Ohba
authors.forename: Shinichi
authors.initials: S
authors.lastname: Iwai
authors.forename: Minoru
authors.initials: M
authors.lastname: Watanabe
authors.forename: Naoki
authors.initials: N
authors.lastname: Matsumoto
authors.forename: Katsuji
authors.initials: K
authors.lastname: Oguchi
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism / Q000503:physiopathology*; D001794:Blood Pressure / Q000235:genetics / Q000502:physiology; D004195:Disease Models, Animal; D015870:Gene Expression / Q000235:genetics* / Q000502:physiology; D006949:Hyperlipidemias / Q000150:complications / Q000235:genetics* / Q000503:physiopathology; D006973:Hypertension / Q000150:complications / Q000235:genetics* / Q000503:physiopathology; D020411:Oligonucleotide Array Sequence Analysis
keywords: Atherosclerosis; Esm1; Grem2; Microarray analysis; Mkks; Retnlg