title: Biphasic calcium phosphate loading on polycaprolactone/poly(lacto-co-glycolic acid) membranes for improved tensile strength, in vitro biocompatibility, and in vivo tissue regeneration.
abstract: Electrospun polycaprolactone and poly(lacto-co-glycolide) membranes were loaded with biphasic calcium phosphate powder to facilitate osteoconductivity. Different concentrations of biphasic calcium phosphate powder were added to the polymer solution, and successful loading was confirmed by X-ray diffraction analysis, transmission electron microscope, and scanning electron microscope with energy-dispersive spectroscopy visualization. The effect of the added biphasic calcium phosphate on the polymer membrane was investigated in terms of the material's tensile strength and strain, in vitro cytocompatibility, and in vivo tissue regeneration. It was observed that the tensile strength of the membranes increased with the addition of the biphasic calcium phosphate powder. Immersion in simulated body fluid solution for seven days leads to the formation of apatite-like deposits in the fibers, which further improved the mechanical stability. Moreover, proliferation and adhesion of osteoblast-like cells were more apparent upon the addition of the biphasic calcium phosphate powder as seen with the increasing cell density from (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and micrographs from scanning electron microscope and confocal microscopy. Sample membranes were also implanted to investigate the membrane's ability to regenerate bone in a rat calvarium. Histological staining and micro-CT histomorphometric analyses showed neo-bone formation in the implanted rat skull.
authors.affiliation: 1Department of Biomedical Engineering and Materials, Soonchunhyang University, 366-1, Ssangyoung-dong, Cheonan, Chungnam 330-090, South Korea.
authors.forename: Rose Ann
authors.initials: RA
authors.lastname: Franco
authors.forename: Alexander
authors.initials: A
authors.lastname: Sadiasa
authors.forename: Hyung Seok
authors.initials: HS
authors.lastname: Seo
authors.forename: Byong-Taek
authors.initials: BT
authors.lastname: Lee
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000008:administration & dosage* / Q000737:chemistry* / Q000633:toxicity; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D006882:Hydroxyapatites / Q000008:administration & dosage* / Q000737:chemistry / Q000633:toxicity; D019344:Lactic Acid / Q000737:chemistry* / Q000633:toxicity; D008297:Male; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D057139:Nanofibers / Q000737:chemistry / Q000633:toxicity / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D011091:Polyesters / Q000737:chemistry* / Q000633:toxicity; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000633:toxicity; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D033362:Powder Diffraction; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000187:drug effects / Q000502:physiology; D013718:Tensile Strength
chemical_list: D018786:Bone Substitutes; D006882:Hydroxyapatites; D011091:Polyesters; C074950:hydroxyapatite-beta tricalcium phosphate; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; C016240:polycaprolactone; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: BCP; electrospinning; in vitro and in vivo biocompatibility; tensile strength