title: Study on the interaction between gliadins and a coumarin as molecular model system of the gliadins-anthocyanidins complexes.
abstract: To clarify the conformational changes of gliadins (Glia) upon complexation with anthocyanidins (in particular cyanidin, Cya), the interaction of Glia with a coumarin derivative (3-ethoxycarbonylcoumarin, 3-EcC), having a benzocondensed structure similar to that of Cya, has been investigated by NMR, IR, and Raman spectroscopy under acidic and neutral conditions. Raman spectra showed that both molecules produce a similar effect on the Glia structure, i.e. an increase in the α-helix conformation and a decrease in β-sheet and β-turns content. In the presence of both molecules, this effect is more marked; the spectroscopic results showed that both Cya and 3-EcC interact with Glia and 3-EcC favors the complex formation with Glia. The results obtained in this study provide new insights into anthocyanidins-Glia interactions and may have relevance to human health, in the field of the attempts to modify gluten proteins to decrease allergen immunoreactivity.
authors.affiliation: Dipartimento di Scienze Biomediche e Neuromotorie, Università di Bologna, Via Belmeloro 8/2, 40126 Bologna, Italy. silvia.tozzi@unibo.it
authors.forename: Silvia
authors.initials: S
authors.lastname: Tozzi
authors.forename: Nicola
authors.initials: N
authors.lastname: Zanna
authors.forename: Paola
authors.initials: P
authors.lastname: Taddei
mesh_terms: D000872:Anthocyanins / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry*; D005903:Gliadin / Q000737:chemistry*; D008958:Models, Molecular; D011485:Protein Binding; D017433:Protein Structure, Secondary; D013059:Spectrum Analysis, Raman
chemical_list: D000872:Anthocyanins; D003374:Coumarins; D005903:Gliadin; C030123:coumarin
keywords: Anthocyanidin; Coumarin; DFT calculations; Gliadins; NMR spectroscopy; Raman spectroscopy