title: The effects of early life Pb exposure on the expression of IL1-β, TNF-α and Aβ in cerebral cortex of mouse pups.
abstract: OBJECTIVE
  To investigate the effects of maternal lead (Pb) exposure on the learning and memory ability and expression of interleukin1-β (IL1-β), tumor necrosis factor (TNF-α) and beta amyloid protein (Aβ) in cerebral cortex of mice offspring.
  
  
  METHODS
  Pb exposure initiated from beginning of gestation to weaning. Pb acetate administered in drinking solutions was dissolved in distilled deionized water at the concentrations of 0.1%, 0.5% and 1% groups, respectively. On the PND21, the learning and memory ability were tested by water maze test and the Pb levels were also determined by graphite furnace atomic absorption spectrometry. The expression of IL1-β, TNF-α and Aβ in cerebral cortex was measured by immunohistochemistry and western blotting.
  
  
  RESULTS
  The Pb levels in blood and cerebral cortex of all exposure groups were significantly higher than that of the control group (P<0.05). In water maze test, the performances of 0.5% and 1% groups were worse than that of the control group (P<0.05). The expression of IL1-β, TNF-α and Aβ was increased in Pb exposed groups than that of the control group (P<0.05).
  
  
  CONCLUSIONS
  The high expression of IL1-β, TNF-α and Aβ in the cerebral cortex of pups may contribute to the impairment of learning and memory associated with maternal Pb exposure.
authors.affiliation: Food Science Technology College of Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
authors.forename: FangLi
authors.initials: F
authors.lastname: Liu
authors.affiliation: Food Science Technology College of Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: LianJun
authors.initials: L
authors.lastname: Song
authors.affiliation: Food Science Technology College of Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: PingAn
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Food Science Technology College of Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: MingWu
authors.initials: M
authors.lastname: Qiao
authors.affiliation: Food Science Technology College of Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: QiuYan
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: College of Public Health, Zhengzhou University, Zhengzhou 450001, China. Electronic address: lwj@zzu.edu.cn.
authors.forename: WenJie
authors.initials: W
authors.lastname: Li
mesh_terms: D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000378:metabolism*; D007854:Lead / Q000633:toxicity*; D007858:Learning / Q000187:drug effects; D018811:Maternal Exposure / Q000009:adverse effects; D008568:Memory / Q000187:drug effects; D051379:Mice; D011247:Pregnancy; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D007854:Lead
keywords: Aβ; Cerebral cortex; IL1-β; Lead (Pb); TNF-α