title: [Sensitization and oral challenge with ovoalbumin in an animal model of food allergy].
abstract: BACKGROUND
  Gut-associated lymphoid tissue (GALT) is mainly formed by the gut mucosa and associated lymphatic structures that under normal conditions induces hyporesponsiveness, a phenomenon termed oral tolerance. However, the potential brakeup of oral tolerance could otherwise lead to disorders such as food allergy.
  
  
  OBJECTIVE
  The aim of the study is to characterise the histopathological and immunohistochemical modifications in intestinal gut mucosa in an animal model of food allergy.
  
  
  METHODS
  New Zealand rabbits were subcutaneously sensitized twice with ovalbumin (OVA), on day 30 after first sensitization, animals were oral challenged with the same antigen. Lymphatic cell population and accessory cells from gut mucosa were studied by conventional histology, histochemistry and immunohistochemistry.
  
  
  RESULTS
  An important increase in number of eosinophils were observed in sensitized and challenged group as well as CD25+cells increase in sensitized animals without challenge.
  
  
  CONCLUSIONS
  Data obtained demonstrated that subcutaneous sensitization and challenge with OVA induced generation of specific IgE antibodies and an anaphylactic inflammatory response. This pattern induced quantitative modifications in studied cells and structural changes in mucosa like oedema at intestinal villi in sensitized and challenged rabbits in this animal model of food allergy.
authors.affiliation: Laboratorio de InmunohistoquÌmica, Facultad de Ciencias MÈdicas, Universidad Nacional de Rosario, Rosario, Santa Fe, Argentina. vinuesamiguel@gmail.com.
authors.forename: Miguel Ngel
authors.initials: MN
authors.lastname: Vinuesa
authors.forename: Norberto David
authors.initials: ND
authors.lastname: Bassan
authors.forename: Soledad
authors.initials: S
authors.lastname: Chaparro
authors.forename: Adriel
authors.initials: A
authors.lastname: Martìnez
authors.forename: Rocìo
authors.initials: R
authors.lastname: Batle
authors.forename: Florencia
authors.initials: F
authors.lastname: Giacomozzi
authors.forename: Valentìn
authors.initials: V
authors.lastname: Torres
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D004195:Disease Models, Animal; D005512:Food Hypersensitivity / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D010047:Ovalbumin
chemical_list: D000485:Allergens; D007073:Immunoglobulin E; D010047:Ovalbumin