title: [High concentration uric acid regulates endothelial function via miR-155].
abstract: OBJECTIVE
  To investigate high-concentration uric acid-induced endothelial dysfunction mediated by miR-155.
  
  
  METHODS
  Human umbilical vein endothelial cells (HUVECs) were incubated with 600 µmol/L uric acid for 24 and 48 h, and eNOS expression and NO content in the cell culture were measured. The target genes of miR-155 were predicted using on-line analysis software and validated by dual-luciferase system. Real-time PCR was used to detect the expression of miR-155 in endothelial cells incubated with high-concentration uric acid. The effect of miR-155 on endothelial dysfunction was assessed by transfection of its inhibitor into HUVECs.
  
  
  RESULTS
  The expression of eNOS and NO secretion decreased obviously in HUVECs incubated with 600 µmol/L uric acid. MicroRNA on-line analysis software and dual luciferase reporter experiments suggested that the level of eNOS translation was directly regulated by miR-155. The expression of miR-155 in endothelial cells was upregulated after stimulation with high-concentration uric acid, and was inhibited by transfection of miR-155 inhibitor. Expression of eNOS and secretion of NO were elevated in endothelial cells transfected with miR-155 inhibitor after incubation with high-concentration uric acid.
  
  
  CONCLUSIONS
  High-concentration uric acid can down-regulate eNOS expression via miR-155 to induce endothelial dysfunction.
authors.affiliation: Department of Nephrology, State Key Laboratory of Kidney Disease, General Hospital of PLA, Beijing 100853, China. E-mail: xg301301@163.com.
authors.forename: Xueguang
authors.initials: X
authors.lastname: Zhang
authors.forename: Quan
authors.initials: Q
authors.lastname: Hong
authors.forename: Kai
authors.initials: K
authors.lastname: Hou
authors.forename: Yuanda
authors.initials: Y
authors.lastname: Wang
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Chen
mesh_terms: D002478:Cells, Cultured; D015536:Down-Regulation; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D033461:Hyperuricemia / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D014527:Uric Acid / Q000097:blood*
chemical_list: C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D014527:Uric Acid; D009569:Nitric Oxide; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III