title: Development and evaluation of pluronic- and methylcellulose-based thermoreversible drug delivery system for insulin.
abstract: The objective of the current work was to develop and evaluate thermoreversible subcutaneous drug delivery system for Insulin. Thermoreversible in-situ gel system was developed and evaluated both in-vitro and in-vivo comprising of pluronic F-127 alone or in combination with methylcellulose in different ratios. The drug release kinetics and mechanism was predicted by applying various mathematical models to the in-vitro dissolution data. Rabbits were used as animal model following subcutaneous injection to predict various pharmacokinetic parameters by applying Pk-Summit® software. The in-vitro and in-vivo data revealed that the formulation IPM 15/3 consisting of the pluronic F-127 (15% w/v) and methylcellulose (3% w/v) was the most robust and capable formulation for extending the drug release and maintaining basal plasma insulin level between 10 and 40 µU/ml for 240 h (10 d).
authors.affiliation: Department of Pharmacy and.
authors.forename: Fazli
authors.initials: F
authors.lastname: Nasir
authors.forename: Zafar
authors.initials: Z
authors.lastname: Iqbal
authors.forename: Amirzada
authors.initials: A
authors.lastname: Khan
authors.forename: Jamshaid A
authors.initials: JA
authors.lastname: Khan
authors.forename: Abad
authors.initials: A
authors.lastname: Khan
authors.forename: Fazli
authors.initials: F
authors.lastname: Khuda
authors.forename: Shahida
authors.initials: S
authors.lastname: Zakir
authors.forename: Nageen
authors.initials: N
authors.lastname: Yousaf
authors.forename: Ismail
authors.initials: I
authors.lastname: Khan
authors.forename: Yasar
authors.initials: Y
authors.lastname: Shah
authors.forename: Muhammad Imran
authors.initials: MI
authors.lastname: Khan
authors.forename: Naila
authors.initials: N
authors.lastname: Shahbaz
mesh_terms: D000818:Animals; D016503:Drug Delivery Systems; D065546:Drug Liberation; D005782:Gels / Q000737:chemistry*; D007004:Hypoglycemic Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D007279:Injections, Subcutaneous; D007328:Insulin / Q000008:administration & dosage* / Q000493:pharmacokinetics; D008747:Methylcellulose / Q000737:chemistry*; D020442:Poloxamer / Q000737:chemistry*; D011817:Rabbits; D013678:Technology, Pharmaceutical; D014783:Viscosity
chemical_list: D005782:Gels; D007004:Hypoglycemic Agents; D007328:Insulin; D020442:Poloxamer; D008747:Methylcellulose
keywords: Insulin; methylcellulose; pluronics; subcutaneous; thermoreversible in-situ gels