title: Controlled cell adhesion on poly(dopamine) interfaces photopatterned with non-fouling brushes.
abstract: Bioinspired poly(dopamine) (PDA) films are merged with antifouling poly(MeOEGMA) brushes utilizing a nitrile imine-mediated tetrazole-ene cycloaddition (NITEC)-based phototriggered surface encoding protocol. The antifouling brushes were photopatterned on PDA surfaces, leading cells to form confluent layers in the non-irradiated sections, while no adhesion occurred on the brushes resulting in a remarkably precise cell pattern. The presented strategy paves the way for the design of tailor-made patterned cell interfaces.
authors.affiliation: Preparative Macromolecular Chemistry, Institut für Technische Chemie und Polymerchemie, Karlsruhe Institute of Technology (KIT), Engesserstrasse 18, 76131, Karlsruhe, Germany; Zell- und Neurobiologie, Zoologisches Institut, Karlsruhe Institute of Technology (KIT), Haid-und-Neu-Str. 9, Karlsruhe and Institut für Funktionelle Grenzflächen (IFG), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Cesar
authors.initials: C
authors.lastname: Rodriguez-Emmenegger
authors.forename: Corinna M
authors.initials: CM
authors.lastname: Preuss
authors.forename: Basit
authors.initials: B
authors.lastname: Yameen
authors.forename: Ognen
authors.initials: O
authors.lastname: Pop-Georgievski
authors.forename: Michael
authors.initials: M
authors.lastname: Bachmann
authors.forename: Jan O
authors.initials: JO
authors.lastname: Mueller
authors.forename: Michael
authors.initials: M
authors.lastname: Bruns
authors.forename: Anja S
authors.initials: AS
authors.lastname: Goldmann
authors.forename: Martin
authors.initials: M
authors.lastname: Bastmeyer
authors.forename: Christopher
authors.initials: C
authors.lastname: Barner-Kowollik
mesh_terms: D000179:Acrylates / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007211:Indoles / Q000737:chemistry* / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D012825:Silicon / Q000737:chemistry; D000098662:Vasodilator-Stimulated Phosphoprotein
chemical_list: D000179:Acrylates; D015815:Cell Adhesion Molecules; D007211:Indoles; D008840:Microfilament Proteins; D010750:Phosphoproteins; D011108:Polymers; D011993:Recombinant Fusion Proteins; C530664:poly(oligoethylene glycol methyl ether acrylate); C568283:polydopamine; D000098662:Vasodilator-Stimulated Phosphoprotein; D049452:Green Fluorescent Proteins; D011092:Polyethylene Glycols; D012825:Silicon
keywords: antifouling polymer brushes; micropatterning; nitrile imine-mediated tetrazole-ene cycloaddition (NITEC); photoligation; poly(dopamine)