title: ERK and RSK regulate distinct steps of a cellular program that induces transition from multicellular epithelium to single cell phenotype.
abstract: The ERK (extracellular signal-regulated kinases) cascade has an evolutionarily conserved three tier architecture consisting of protein kinases Raf, MEK (MAPK/ERK kinase) and ERK. Following activation, ERK phosphorylates various cellular elements leading to diverse cellular responses. Downstream of ERK the family of p90 ribosomal S6 kinases (RSKs) has been proven to be an important conveyor of ERK signaling, however, little is known if ERK and RSK coordinate their functions to generate a specific biological response. Here we show that in epithelial cells conditional activation of the ERK pathway causes phenotypic conversion of epithelial cells to autonomously migrating cells. This process involves two sequential steps characterized by loss of apical-basal polarity followed by cell scattering. The activation of ERK, but not RSK, is sufficient for the execution of the first step and it requires calpain mediated remodeling of actin cytoskeleton. Conversely, RSK regulates the successive stage characterized by cell-cell contact weakening and increased cellular migration. Thus, ERK and RSK regulate different cellular subprograms and coordinated execution of these subprograms in time generates a relevant biological response. Our data also suggest that the mechanism by which the ERK pathway controls a cellular response may be distributed between ERK and RSK, rather than being elicited by a single effector kinase.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Videňská 1083, Prague, Czech Republic.
authors.forename: Josef
authors.initials: J
authors.lastname: Čáslavský
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Klímová
authors.forename: Tomáš
authors.initials: T
authors.lastname: Vomastek
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002154:Calpain / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement; D016764:Cell Polarity; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D006801:Humans; D007365:Intercellular Junctions / Q000378:metabolism; D020935:MAP Kinase Signaling System; D038744:Ribosomal Protein S6 Kinases, 90-kDa / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D015820:Cadherins; D038744:Ribosomal Protein S6 Kinases, 90-kDa; D048049:Extracellular Signal-Regulated MAP Kinases; D002154:Calpain
keywords: 4-hydroxytamoxifen; 4HT; ALLM; ALLN; Calpain; Cell–cell junctions; EMT; ER; MAPK/ERK; MDCK; Madin–Darby Canine Kidney; Migration; N-Acetyl-l-leucine-l-leucine-l-norleucinal; N-Acetyl-l-leucyl-l-leucyl-l-methional; Polarity; RSK; epithelial–mesenchymal transition; ligand binding domain of estrogen receptor