title: Effect of methylcellulose on the formation and drug release behavior of silk fibroin hydrogel.
abstract: In this study, methylcellulose (MC) was used to control the gelation time of silk fibroin (SF) aqueous solution. The gelation time was measured using a Vibro Viscometer at 50 °C. Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and a texture meter were used to investigate the effect of MC on the hydrogelation of SF solution. SF/MC hydrogels could be formed by the addition of MC, although their gelation time was increased with MC content. To examine the conformational change of SF/MC hydrogels, time-resolved FT-IR spectra were obtained at constant temperature using a custom-made IR chamber. From FT-IR spectra focused on the amide I peak position, the transition of SF molecules in SF/MC solution from a random coil to a β-sheet structure was inhibited in the presence of MC molecules. In addition, the drug release of SF/MC hydrogels loaded with 5-aminosalicylic acid was studied in 2-dimensional (2-D) and 3-dimensional (3-D) conditions in vitro. The drug release behavior of SF or SF/MC hydrogels was measured using UV-Vis spectroscopy. The release rate of 5-aminosalicylic acid in SF/MC hydrogel was lower than that of SF hydrogel, which may be closely associated with the hydrophilic interaction between MC and 5-aminosalicylic acid. This approach to controlling the sol-gel transition and the drug release of SF hydrogels by the addition of MC will be useful in the design and tailoring of novel materials for biomedical applications.
authors.affiliation: Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University, Daejeon 305-764, South Korea.
authors.forename: Cho Hee
authors.initials: CH
authors.lastname: Park
authors.forename: Lim
authors.initials: L
authors.lastname: Jeong
authors.forename: Donghwan
authors.initials: D
authors.lastname: Cho
authors.forename: Oh Hyeong
authors.initials: OH
authors.lastname: Kwon
authors.forename: Won Ho
authors.initials: WH
authors.lastname: Park
mesh_terms: D000818:Animals; D004337:Drug Carriers / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D019804:Mesalamine / Q000737:chemistry; D008747:Methylcellulose / Q000737:chemistry*; D012996:Solutions; D014867:Water / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D020100:Hydrogels; D012996:Solutions; D014867:Water; D019804:Mesalamine; D008747:Methylcellulose; D005349:Fibroins
keywords: Drug release; Gelation; Methylcellulose; Silk fibroin