title: Three new neuritogenic steroidal saponins from Ophiopogon japonicus (Thunb.) Ker-Gawl.
abstract: Three new steroidal saponins (1-3) and a known saponin (4) were isolated from Ophiopogon japonicus (Thunb.) Ker-Gawl. Their structures were determined by spectroscopic analyses and chemical derivatization. The isolated compounds (1-4) were potent inducers of neuritogenesis on PC12 cells. Compound 1 showed the highest neuritogenic activity of 46% at 1 μM. The study of structure-activity relationships suggests that aglycone is important for the neuritogenic activity of the compounds. Specific inhibitor experiments and Western blot analysis suggest that 1-induced neuritogenic activity depends on the activation of mitogen-activated protein kinase kinase (MEK)/extracellular signal regulated kinase (ERK) signaling pathway on PC12 cells.
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Yu Hang Tang Road 866, Hangzhou 310058, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ye
authors.forename: Yuan
authors.initials: Y
authors.lastname: Qu
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Tang
authors.forename: Shining
authors.initials: S
authors.lastname: Cao
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.forename: Lan
authors.initials: L
authors.lastname: Xiang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Qi
mesh_terms: D000818:Animals; D020935:MAP Kinase Signaling System; D016501:Neurites / Q000187:drug effects / Q000502:physiology*; D031441:Ophiopogon / Q000737:chemistry*; D016716:PC12 Cells; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: C000590051:25-ruscogenin 1-O-xylopyranosyl-(1-2)-(xylopyranosyl-(1-3))fucopyranoside; D010936:Plant Extracts; D012503:Saponins
keywords: ERK phosphorylation; Neuritogenic activity; Ophiopogon japonicus; Steroidal saponins