title: A kinetic study of biohydrogen production from glucose, molasses and cheese whey by suspended and attached cells of Thermotoga neapolitana.
abstract: Batch tests of H2 production from glucose, molasses and cheese whey by suspended and immobilized cells of Thermotoga neapolitana were conducted to develop a kinetic model of the process. H2 production was inhibited by neither H2 (up to 0.7 mg L(-1)) nor O2 (up to 0.2 mg L(-1)). The H2 specific rates obtained at different substrate concentrations were successfully interpolated with Andrew's inhibition model. With glucose and molasses, biofilms performed better than suspended cells. The suspended-cell process was successfully scaled-up to a 19-L bioreactor. Assays co-fed with molasses and cheese whey led to higher H2 productivities and H2/substrate yields than the single-substrate tests. The simulation of the suspended-cell continuous-flow process indicated the potential attainment of H2 productivities higher than those of the batch tests (up to 3.6 mmol H2 h(-1) L(-1) for molasses and 0.67 mmol H2 h(-1) L(-1) for cheese whey) and allowed the identification of the optimal dilution rate.
authors.affiliation: Department of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, Via Terracini 28, Bologna, Italy. Electronic address: dario.frascari@unibo.it.
authors.forename: Dario
authors.initials: D
authors.lastname: Frascari
authors.affiliation: Department of Pharmacy and BioTechnology, University of Bologna, Via Irnerio 42, Bologna, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Cappelletti
authors.affiliation: Department of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, Via Terracini 28, Bologna, Italy.
authors.forename: Jocelia De Sousa
authors.initials: JS
authors.lastname: Mendes
authors.affiliation: Department of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, Via Terracini 28, Bologna, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Alberini
authors.affiliation: Department of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, Via Terracini 28, Bologna, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Scimonelli
authors.affiliation: Department of Pharmacy and BioTechnology, University of Bologna, Via Irnerio 42, Bologna, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Manfreda
authors.affiliation: Department of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, Via Terracini 28, Bologna, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Longanesi
authors.affiliation: Department of Pharmacy and BioTechnology, University of Bologna, Via Irnerio 42, Bologna, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Zannoni
authors.affiliation: Department of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, Via Terracini 28, Bologna, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Pinelli
authors.affiliation: Department of Pharmacy and BioTechnology, University of Bologna, Via Irnerio 42, Bologna, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Fedi
mesh_terms: D002611:Cheese; D005947:Glucose / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D007700:Kinetics; D008965:Molasses; D046949:Thermotoga neapolitana / Q000378:metabolism*
chemical_list: D006859:Hydrogen; D005947:Glucose
keywords: Biofilm; Biohydrogen; Cheese whey; Kinetic study; Molasses