title: [Design and evaluation of a kind of biomimetic peptides of dentin matrix protein-1].
abstract: OBJECTIVE
  To design a kind of biomimetic polypeptide of dentin matrix protein-1 (DMP-1), which can bind to dentine collagen fibers and initiate mineralization.
  
  
  METHODS
  A novel polypeptide was developed by connecting the collagen binding domain of DMP-1 "DSESSEEDR" to the hydrophilic C-terminal of amelogenin "TKREEVD". The polypeptide was synthetically prepared by standard solid-phase peptide synthesis. Human dentine slices were completely demineralized by hydrochloric acid to expose the dentine collagen. Fluorescein isothiocyanate coupled polypeptide was applied to the exposed dentine collagen. Fluorescent microscopy was used to examine the polypeptide specially bond to the dentine collagen. Nucleation and growth of hydroxyapatite was initiated by immersing the polypeptide into calcium chloride and sodium hypophosphate solutions respectively. Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and selected area diffraction (SAD) were used to examine the hydroxyapatites formed. RESULTS Fluorescent dentine collagen was identified in the demineralized dentine specimens. Nucleation and growth of crystals were detected after immersing the polypeptide into calcium chloride and sodium hypophosphate solutions by SEM and TEM. SAD confirmed the crystals were hydroxyapatites.
  
  
  CONCLUSIONS
  The polypeptide of "DSESSEEDRTKREEVD" can simulate DMP-1 binding collagen and initiate hydroxyapatite nucleation and growth. It may be a potential molecular tool for dentine remineralization.
authors.affiliation: Dept. of Prosthodontics, Stomatologic Hospital & College of Anhui Medical University, Key Laboratory of Oral Diseases Research of Anhui Province, Hefei 230032, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Cao
authors.forename: Jun
authors.initials: J
authors.lastname: Shen
authors.forename: Qiangjian
authors.initials: Q
authors.lastname: Xu
authors.forename: Zhenxiong
authors.initials: Z
authors.lastname: Zhu
authors.forename: Quanli
authors.initials: Q
authors.lastname: Li
mesh_terms: D032701:Biomimetics; D002130:Calcium Phosphates; D003094:Collagen; D003804:Dentin; D017886:Durapatite; D006801:Humans; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010455:Peptides
chemical_list: D002130:Calcium Phosphates; D010455:Peptides; D003094:Collagen; D017886:Durapatite