title: SLAT negatively regulates RANKL-induced osteoclast differentiation.
abstract: RANKL induces the formation of osteoclasts, which are responsible for bone resorption. Herein, we investigated the role of SWAP-70-like adapter of T cells (SLAT) in RANKL-induced osteoclastogenesis. Expression levels of SLAT were reduced during RANKL-induced osteoclastogenesis. Overexpression of SLAT in BMMs inhibited TRAP-positive multinuclear osteoclast formation and attenuated the expression of NFATc1, which is an important modulator in osteoclastogenesis. Furthermore, silencing of SLAT by RNA interference enhanced osteoclast formation as well as NFATc1 expression. In addition, SLAT was involved in RANKL-induced JNK activation in osteoclasts. Taken together, our data suggest that SLAT acts as a negative modulator of RANKL-induced osteoclastogenesis.
authors.affiliation: Department of Pharmacology, Medical Research Center for Gene Regulation, Chonnam National University Medical School, Gwangju, 501-746, Korea.
authors.forename: Bang Ung
authors.initials: BU
authors.lastname: Youn
authors.forename: Kabsun
authors.initials: K
authors.lastname: Kim
authors.forename: Jung Ha
authors.initials: JH
authors.lastname: Kim
authors.forename: Jongwon
authors.initials: J
authors.lastname: Lee
authors.forename: Jang Bae
authors.initials: JB
authors.lastname: Moon
authors.forename: Inyoung
authors.initials: I
authors.lastname: Kim
authors.forename: Yong-Wook
authors.initials: YW
authors.lastname: Park
authors.forename: Nacksung
authors.initials: N
authors.lastname: Kim
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D001862:Bone Resorption / Q000378:metabolism; D019070:Cell Lineage; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D020662:Guanine Nucleotide Exchange Factors; D007527:Isoenzymes / Q000378:metabolism; D020935:MAP Kinase Signaling System; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D050778:NFATC Transcription Factors / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010010:Osteoclasts / Q000166:cytology / Q000502:physiology*; D053245:RANK Ligand / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D000135:Acid Phosphatase; D004268:DNA-Binding Proteins; D020662:Guanine Nucleotide Exchange Factors; D007527:Isoenzymes; D050778:NFATC Transcription Factors; D009687:Nuclear Proteins; D053245:RANK Ligand; D000071681:Tartrate-Resistant Acid Phosphatase; C494005:Nfatc1 protein, mouse; C473249:Def6 protein, mouse; C000605511:Acp5 protein, mouse