title: Isolation of genomic DNA from mammalian cells.
abstract: The isolation of genomic DNA from mammalian cells is a routine molecular biology laboratory technique with numerous downstream applications. The isolated DNA can be used as a template for PCR, cloning, and genotyping and to generate genomic DNA libraries. It can also be used for sequencing to detect mutations and other alterations, and for DNA methylation analyses.
authors.affiliation: Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: ckoh6@jhmi.edu.
authors.forename: Cheryl M
authors.initials: CM
authors.lastname: Koh
mesh_terms: D000818:Animals; D004247:DNA / Q000302:isolation & purification*; D016678:Genome; D008322:Mammals / Q000235:genetics*
chemical_list: D004247:DNA
keywords: Cell lysis; Collection of mammalian cells; Ethanol precipitation; Isolation of genomic DNA; Mammalian cells; Organic extraction