title: Anti-inflammatory and antinociceptive activity of chitin-binding lectin from Canna limbata seeds.
abstract: Lectins are a structurally heterogeneous group of proteins or glycoproteins with at least one noncatalytic domain binding reversibly to a specific mono- or oligosaccharide. Monocot mannose-binding lectins are an extended superfamily of structurally and evolutionarily related proteins. In this study, we evaluated anti-inflammatory and antinociceptive effects of monocot lectin from the Canna limbata seeds (CLL). To accomplish this, CLL was purified and subjected to pharmacological assays: abdominal writhing induced by acetic acid, formalin, hot plate and Zymosan A-induced peritonitis tests. The CLL was purified by chromatographic chitin column, and the relative mass of 21 kDa observed in electrophoresis was confirmed by electrospray mass spectrometry, which also revealed that purified CLL consists of a dimer having a weight of 49,676 Da. The CLL showed nociceptive activity in the acetic acid test as well as peripheral antinociceptive response. The CLL also showed anti-inflammatory effect with the reduction of inflammation in the formalin test and neutrophil migration into the peritoneal cavity. This is the first report of anti-inflammatory activity for a monocot lectin, and it suggests a new pharmacological tool to understand inflammatory and antinociceptive processes mediated through lectins.
authors.affiliation: Laboratório de Farmacologia e Imunidade (LaFI), Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Alagoas, Maceió, AL, Brazil.
authors.forename: Theolyta S
authors.initials: TS
authors.lastname: Araújo
authors.forename: Claudener S
authors.initials: CS
authors.lastname: Teixeira
authors.forename: Maria A P
authors.initials: MA
authors.lastname: Falcão
authors.forename: Vanir R Pinto
authors.initials: VR
authors.lastname: Junior
authors.forename: Mayara Quiroz
authors.initials: MQ
authors.lastname: Santiago
authors.forename: Raquel G
authors.initials: RG
authors.lastname: Benevides
authors.forename: Plínio
authors.initials: P
authors.lastname: Delatorre
authors.forename: Jorge L
authors.initials: JL
authors.lastname: Martins
authors.forename: Magna S
authors.initials: MS
authors.lastname: Alexandre-Moreira
authors.forename: Benildo S
authors.initials: BS
authors.lastname: Cavada
authors.forename: Eliane A
authors.initials: EA
authors.lastname: Campesatto
authors.forename: Bruno A M
authors.initials: BA
authors.lastname: Rocha
mesh_terms: D000700:Analgesics / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002686:Chitin / Q000378:metabolism*; D005557:Formaldehyde / Q000633:toxicity; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D037102:Lectins / Q000378:metabolism*; D051379:Mice; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011485:Protein Binding; D012639:Seeds / Q000737:chemistry*; D019862:Zingiberales / Q000737:chemistry; D015054:Zymosan / Q000633:toxicity
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D037102:Lectins; D010936:Plant Extracts; D002686:Chitin; D005557:Formaldehyde; D015054:Zymosan