title: The presence of disease-associated prion protein in skeletal muscle of cattle infected with classical bovine spongiform encephalopathy.
abstract: The aim of this study was to investigate the presence of disease-associated prion protein (PrP(Sc)) in the skeletal muscle of cattle infected with classical bovine spongiform encephalopathy (C-BSE). The study was carried out systematically in 12 different muscle samples from 43 (3 field and 40 experimental) cases of C-BSE; however, muscle spindles were not available in many of these cases. Therefore, analysis became restricted to a total of 31 muscles in 23 cattle. Even after this restriction, low levels of PrP(Sc) were detected in the muscle spindles of the masseter, intercostal, triceps brachii, psoas major, quadriceps femoris and semitendinosus muscles from 3 field and 6 experimental clinical-stage cases. The present data indicate that small amounts of PrP(Sc) are detectable by immunohistochemistry in the skeletal muscles of animals terminally affected with C-BSE.
authors.affiliation: National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Okada
authors.forename: Kohtaro
authors.initials: K
authors.lastname: Miyazawa
authors.forename: Shigeo
authors.initials: S
authors.lastname: Fukuda
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Iwamaru
authors.forename: Morikazu
authors.initials: M
authors.lastname: Imamura
authors.forename: Kentaro
authors.initials: K
authors.lastname: Masujin
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Matsuura
authors.forename: Takashi
authors.initials: T
authors.lastname: Fujii
authors.forename: Kei
authors.initials: K
authors.lastname: Fujii
authors.forename: Soichi
authors.initials: S
authors.lastname: Kageyama
authors.forename: Miyako
authors.initials: M
authors.lastname: Yoshioka
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Murayama
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokoyama
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle; D016643:Encephalopathy, Bovine Spongiform / Q000473:pathology*; D018482:Muscle, Skeletal / Q000473:pathology*; D018620:PrPSc Proteins / Q000032:analysis*
chemical_list: D018620:PrPSc Proteins