title: Screening of extracts from natural feed ingredients for their ability to reduce enterotoxigenic Escherichia coli (ETEC) K88 adhesion to porcine intestinal epithelial cell-line IPEC-J2.
abstract: Enterotoxigenic Escherichia coli (ETEC) K88 is the most prevalent enteropathogen in weaned piglets, with the ability to express fimbria F4 and specifically attach to intestinal receptors in the young piglet. The prevention of ETEC K88 adhesion to the epithelium by interfering in this fimbria-receptor recognition provides an alternative approach to prevent the initial stage of disease. The aim of this study is to screen, among different feed ingredients (FI), their ability to reduce ETEC K88 attachment to the porcine intestinal epithelial cell-line (IPEC-J2). The selected FI consisted of products of a vegetable or dairy origin, and microbial by-products, which could be suitable to be included in piglet's diet. Incubation of a mixture of each FI extract with the bacteria on IPEC-J2 monolayer was allowed. After washing with PBS to remove the non-adhered bacteria, the culture medium was added to grow the adhered bacteria and, simultaneously, to keep the cells alive. Then, the bacterial growth was monitored in a spectrophotometer reader for 12h. Casein glycomacropeptide (CGMP), locust bean (LB), exopolysaccharide (EPS) and wheat bran (WB) reduced the number of attached ETEC K88 to IPEC-J2, but no anti-adhesive effect was found for soybean hulls, sugar-beet pulp, locust gum, fructooligosaccharides, inulin, mushroom, mannanoligosaccharides or the fermented product from Aspergillus oryzae. The lineal analysis of dose responses demonstrated lineal activity (P<0.0001) for CGMP, LB, EPS and WB. These in vitro results suggest CGMP, LB, EPS and WB as good candidates to be included in piglet's diet with supported functional activity against colibacillosis.
authors.affiliation: Servei de Nutrició i Benestar Animal (SNiBA), Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Barcelona, Spain. Electronic address: gemma.gonzalez.ortiz@gmail.com.
authors.forename: G
authors.initials: G
authors.lastname: González-Ortiz
authors.forename: R G
authors.initials: RG
authors.lastname: Hermes
authors.forename: R
authors.initials: R
authors.lastname: Jiménez-Díaz
authors.forename: J F
authors.initials: JF
authors.lastname: Pérez
authors.forename: S M
authors.initials: SM
authors.lastname: Martín-Orúe
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects*; D002460:Cell Line; D054307:Enterotoxigenic Escherichia coli / Q000502:physiology*; D004847:Epithelial Cells / Q000382:microbiology*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D010861:Fimbriae, Bacterial / Q000378:metabolism; D007422:Intestines / Q000382:microbiology; D010936:Plant Extracts / Q000494:pharmacology*; D011134:Polysaccharides / Q000494:pharmacology; D013234:Stem Cells; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology / Q000517:prevention & control*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D011134:Polysaccharides
keywords: IPEC-J2 cell-line, Enterotoxigenic E. coli K88, Fimbriae, Natural feed ingredients, Anti-adhesive therapies, Colibacillosis