title: Molecular basis of tubulin transport within the cilium by IFT74 and IFT81.
abstract: Intraflagellar transport (IFT) of ciliary precursors such as tubulin from the cytoplasm to the ciliary tip is involved in the construction of the cilium, a hairlike organelle found on most eukaryotic cells. However, the molecular mechanisms of IFT are poorly understood. Here, we found that the two core IFT proteins IFT74 and IFT81 form a tubulin-binding module and mapped the interaction to a calponin homology domain of IFT81 and a highly basic domain in IFT74. Knockdown of IFT81 and rescue experiments with point mutants showed that tubulin binding by IFT81 was required for ciliogenesis in human cells.
authors.affiliation: Department of Structural Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.
authors.forename: Sagar
authors.initials: S
authors.lastname: Bhogaraju
authors.forename: Lukas
authors.initials: L
authors.lastname: Cajanek
authors.forename: Cécile
authors.initials: C
authors.lastname: Fort
authors.forename: Thierry
authors.initials: T
authors.lastname: Blisnick
authors.forename: Kristina
authors.initials: K
authors.lastname: Weber
authors.forename: Michael
authors.initials: M
authors.lastname: Taschner
authors.forename: Naoko
authors.initials: N
authors.lastname: Mizuno
authors.forename: Stefan
authors.initials: S
authors.lastname: Lamla
authors.forename: Philippe
authors.initials: P
authors.lastname: Bastin
authors.forename: Erich A
authors.initials: EA
authors.lastname: Nigg
authors.forename: Esben
authors.initials: E
authors.lastname: Lorentzen
mesh_terms: D045744:Cell Line, Tumor; D016825:Chlamydomonas reinhardtii / Q000235:genetics / Q000378:metabolism; D002923:Cilia / Q000235:genetics / Q000502:physiology*; D018360:Crystallography, X-Ray; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D006801:Humans; D009124:Muscle Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017354:Point Mutation; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D034741:RNA, Small Interfering / Q000235:genetics; D014404:Tubulin / Q000378:metabolism*
chemical_list: D003598:Cytoskeletal Proteins; C490080:IFT74 protein, human; C481012:IFT81 protein, human; D009124:Muscle Proteins; D010940:Plant Proteins; D034741:RNA, Small Interfering; D014404:Tubulin