title: Protein interactions between surface annexin A2 and S100A10 mediate adhesion of breast cancer cells to microvascular endothelial cells.
abstract: Annexin A2 (AnxA2) and S100A10 are known to form a molecular complex. Using fluorescence-based binding assays, we show that both proteins are localised on the cell surface, in a molecular form that allows mutual interaction. We hypothesized that binding between these proteins could facilitate cell-cell interactions. For cells that express surface S100A10 and surface annexin A2, cell-cell interactions can be blocked by competing with the interaction between these proteins. Thus an annexin A2-S100A10 molecular bridge participates in cell-cell interactions, revealing a hitherto unexplored function of this protein interaction.
authors.affiliation: School of Pharmacy, Centre for Biomolecular Sciences, University of Nottingham, Nottingham NG7 2RD, United Kingdom.
authors.forename: Helene K
authors.initials: HK
authors.lastname: Myrvang
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Guo
authors.forename: Chan
authors.initials: C
authors.lastname: Li
authors.forename: Lodewijk V
authors.initials: LV
authors.lastname: Dekker
mesh_terms: D017306:Annexin A2 / Q000378:metabolism* / Q000502:physiology; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion / Q000502:physiology*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D005260:Female; D006801:Humans; D011485:Protein Binding; D009418:S100 Proteins / Q000378:metabolism* / Q000502:physiology; D000099248:S100 Calcium Binding Protein A10
chemical_list: D017306:Annexin A2; D009418:S100 Proteins; D000099248:S100 Calcium Binding Protein A10
keywords: 2′,7′-bis-(2-carboxyethyl)-5,6-carboxyfluorescein acetoxymethylester; Annexin; AnxA2; BCECF-AM; Cell–cell interaction; EGM-2; HBME cells; HMEC; HUVEC; Protein interaction; S100 protein; annexin A2; endothelial growth medium-2; human bone marrow endothelial cells; human microvascular endothelial cell; human umbilical vein endothelial cell