title: A two-domain elevator mechanism for sodium/proton antiport.
abstract: Sodium/proton (Na(+)/H(+)) antiporters, located at the plasma membrane in every cell, are vital for cell homeostasis. In humans, their dysfunction has been linked to diseases, such as hypertension, heart failure and epilepsy, and they are well-established drug targets. The best understood model system for Na(+)/H(+) antiport is NhaA from Escherichia coli, for which both electron microscopy and crystal structures are available. NhaA is made up of two distinct domains: a core domain and a dimerization domain. In the NhaA crystal structure a cavity is located between the two domains, providing access to the ion-binding site from the inward-facing surface of the protein. Like many Na(+)/H(+) antiporters, the activity of NhaA is regulated by pH, only becoming active above pH 6.5, at which point a conformational change is thought to occur. The only reported NhaA crystal structure so far is of the low pH inactivated form. Here we describe the active-state structure of a Na(+)/H(+) antiporter, NapA from Thermus thermophilus, at 3 Å resolution, solved from crystals grown at pH 7.8. In the NapA structure, the core and dimerization domains are in different positions to those seen in NhaA, and a negatively charged cavity has now opened to the outside. The extracellular cavity allows access to a strictly conserved aspartate residue thought to coordinate ion binding directly, a role supported here by molecular dynamics simulations. To alternate access to this ion-binding site, however, requires a surprisingly large rotation of the core domain, some 20° against the dimerization interface. We conclude that despite their fast transport rates of up to 1,500 ions per second, Na(+)/H(+) antiporters operate by a two-domain rocking bundle model, revealing themes relevant to secondary-active transporters in general.
authors.affiliation: Division of Molecular Biosciences, Imperial College London, London SW7 2AZ, UK.
authors.forename: Chiara
authors.initials: C
authors.lastname: Lee
authors.forename: Hae Joo
authors.initials: HJ
authors.lastname: Kang
authors.forename: Christoph
authors.initials: C
authors.lastname: von Ballmoos
authors.forename: Simon
authors.initials: S
authors.lastname: Newstead
authors.forename: Povilas
authors.initials: P
authors.lastname: Uzdavinys
authors.forename: David L
authors.initials: DL
authors.lastname: Dotson
authors.forename: So
authors.initials: S
authors.lastname: Iwata
authors.forename: Oliver
authors.initials: O
authors.lastname: Beckstein
authors.forename: Alexander D
authors.initials: AD
authors.lastname: Cameron
authors.forename: David
authors.initials: D
authors.lastname: Drew
mesh_terms: D001224:Aspartic Acid / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D018360:Crystallography, X-Ray; D029968:Escherichia coli Proteins / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D011522:Protons; D012964:Sodium / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D055672:Static Electricity; D016963:Thermus thermophilus / Q000737:chemistry* / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; C102839:NhaA protein, E coli; D011522:Protons; D017923:Sodium-Hydrogen Exchangers; D001224:Aspartic Acid; D012964:Sodium