title: [Study on the effect of extracts from the leaves of Phyllanthus emblica on immune function of mice].
abstract: OBJECTIVE
  To explore the effect of extracts from the leaves of Phyllanthus emblica (PLFs) on the immune function of mice.
  
  
  METHODS
  70 Kunming mice were choosed to conduct the acute toxicity test of PLFs. The mice were randomly divided into four groups: PLFs high-dosage group, mid-dosage group, low-dosage group and control group. The high,mid,low-dosage groups were treated with PLFs 1.982, 0.991 and 0.496 g/kg respectively per day. The same volume of double distilled water was given to the control group. All by intragastric administration for 7 d. The animals were killed and indexes of thymus and spleen were calculated. The expurgation index K and phagocyte index a were detected after the mice being injected with a dilute India ink through caudal vein. In addition, prepared spleen cells conventionally,the activity of Natural Killer cells was measured and the proliferation of T and B cells were detected. The effect of the extracts on serum hemolysin was detected after the SRBC was injected into the enterocoelia.
  
  
  RESULTS
  The LD50 of PLFs was 9. 911 g/kg. Compared with the control group, the indexes of thymus and spleen in the treatment groups had no markedly difference (P > 0.05). The high- and mid-dosage groups could obviously improve the expurgation index K (P < 0.05), phagocyte index alpha (P < 0.05) and NK cell activity (P < 0.05).
  
  
  CONCLUSIONS
  The extracts from Phyllanthus emblica leaves can promote nonspecific immunity immune function in mice.
authors.affiliation: Center of Research & Development of New Drugs, Guangxi University of Chinese Medicine, Nanning 530001, China. zhongzg@gxtcmu.edu.cn
authors.forename: Zhen-Guo
authors.initials: ZG
authors.lastname: Zhong
authors.forename: Xue-Fei
authors.initials: XF
authors.lastname: Luo
authors.forename: Jin-Lan
authors.initials: JL
authors.lastname: Huang
authors.forename: Wen
authors.initials: W
authors.lastname: Cui
authors.forename: Dan
authors.initials: D
authors.lastname: Huang
authors.forename: Ying-Qin
authors.initials: YQ
authors.lastname: Feng
authors.forename: Cai-Ping
authors.initials: CP
authors.lastname: Li
authors.forename: Man-Li
authors.initials: ML
authors.lastname: Li
authors.forename: Zhi-Qi
authors.initials: ZQ
authors.lastname: Huang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D005260:Female; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D007928:Lethal Dose 50; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D008297:Male; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D029842:Phyllanthus emblica / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D023382:Toxicity Tests, Acute
chemical_list: D004365:Drugs, Chinese Herbal