title: Epidermal growth factor can optimize a serum-free culture system for bone marrow stem cell proliferation in a miniature pig model.
abstract: Bone marrow-derived mesenchymal stem cells have become an attractive cell source for periodontal ligament regeneration treatment because of their potential to engraft to several tissue types after injury. Most researchers have focused on the transplantation process, but few have paid attention to cell safety concerns and rapid proliferation before transplantation. Using serum-free medium to culture stem cells may be an effective method to avoid problems associated with exogenous serum and the addition of growth factors to promote cell proliferation. Here, we randomly divided our serum-free cultures and treated them with different levels of epidermal growth factor (EGF). We then evaluated changes in rates of cell adhesion, proliferation, apoptosis, and cell cycle ratio as well as their differentiation potential. The data showed that all of these parameters were significantly different when comparing serum-free cultures with and without 10 nM/L EGF (p < 0.05/0.01); however, cells with 10 nM/L EGF did not respond differently than cells grown in standard serum-containing media without EGF (p > 0.05). In summary, our results demonstrate that 10 nM/L EGF was the optimal dose for serum-free culture, which can replace traditional standard serum medium for in vitro expansion of miniature pig bone marrow-derived mesenchymal stem cells.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University, No. 110 Xiangya Road, Changsha, Hunan, 410078, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Zheng
authors.forename: Ousheng
authors.initials: O
authors.lastname: Liu
authors.forename: Shutao
authors.initials: S
authors.lastname: Zheng
authors.forename: Yishan
authors.initials: Y
authors.lastname: Liu
authors.forename: Yuehong
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhangui
authors.initials: Z
authors.lastname: Tang
authors.forename: Liangjun
authors.initials: L
authors.lastname: Zhong
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D017931:DNA Primers / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine; D013556:Swine, Miniature; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D016895:Culture Media, Serum-Free; D017931:DNA Primers; D013778:Tetrazolium Salts; D013844:Thiazoles; D004815:Epidermal Growth Factor; C022616:thiazolyl blue