title: Effects of imperatorin on nicotine-induced anxiety- and memory-related responses and oxidative stress in mice.
abstract: The purpose of the reported experiments was to examine the effects of imperatorin [9-[(3-methylbut-2-en-1-yl)oxy]-7H-furo[3,2-g]chromen-7-one] on anxiety and memory-related responses induced by nicotine in mice and their relation to the level of nicotine-induced oxidative stress in brain as well as in the hippocampus and the prefrontal cortex. Male Swiss mice were tested for anxiety in the elevated plus maze test (EPM), and for cognition using passive avoidance (PA) procedures. Imperatorin, purified by high-speed counter-current chromatography from methanol extract of fruits of Angelica officinalis, acutely administered at the doses of 10 and 20mg/kg impaired the anxiogenic effect of nicotine (0.1mg/kg, s.c.). Furthermore, acute injections of subthreshold dose of imperatorin (1mg/kg, i.p.) improved processes of memory acquisition when co-administered with nicotine used at non-active dose of 0.05 mg/kg, s.c. Additionally, repeated administration of imperatorin (1mg/kg, i.p., twice daily, for 6 days) improved different stages of memory processes (both acquisition and consolidation) when injected in combination with non-active dose of nicotine (0.05 mg/kg, s.c.) in the PA task. Oxidative stress was assessed by determination of antioxidant enzymes (glutathione peroxidases (GPx), superoxide dismutase (SOD), glutathione reductase (GR)) activities as well as of malondialdehyde (MDA) concentration in the whole brain, the hippocampus and the prefrontal cortex after repeated administration of imperatorin (1mg/kg, 6 days) and single nicotine injection (0.05 mg/kgs.c.) on the seventh day. The results of our research suggest strong behavioural interaction between imperatorin and nicotine at the level of anxiety- and cognitive-like processes. Furthermore, imperatorin inhibited nicotine-induced changes in examined indicators of oxidative stress, especially in the hippocampus and the cortex.
authors.affiliation: Department of Pharmacology and Pharmacodynamics, Medical University of Lublin, Poland. Electronic address: basia.budzynska@am.lublin.pl.
authors.forename: Barbara
authors.initials: B
authors.lastname: Budzynska
authors.forename: Anna
authors.initials: A
authors.lastname: Boguszewska-Czubara
authors.forename: Marta
authors.initials: M
authors.lastname: Kruk-Slomka
authors.forename: Krystyna
authors.initials: K
authors.lastname: Skalicka-Wozniak
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Michalak
authors.forename: Irena
authors.initials: I
authors.lastname: Musik
authors.forename: Grazyna
authors.initials: G
authors.lastname: Biala
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Glowniak
mesh_terms: D000818:Animals; D001007:Anxiety / Q000139:chemically induced*; D001522:Behavior, Animal / Q000187:drug effects; D003071:Cognition / Q000187:drug effects; D011564:Furocoumarins / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008568:Memory / Q000187:drug effects*; D051379:Mice; D009538:Nicotine / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D017397:Prefrontal Cortex / Q000187:drug effects / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D011564:Furocoumarins; D008315:Malondialdehyde; D009538:Nicotine; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; C031534:imperatorin
keywords: Angelica officinalis; Anxiety; Imperatorin; Memory; Mice; Oxidative stress