title: Shuffling of DNA cassettes in a synthetic integron.
abstract: The complexity of even small gene networks makes them hardly amenable to rational design. Testing random combinations of genetic elements in a directed evolution procedure is thus of interest for many applications including metabolic engineering. Here we describe how the recombination machinery of class 1 integrons can be used to deliver and shuffle genetic elements at a chromosomal locus in E. coli.
authors.affiliation: Bacterial Genome Plasticity Unit, Institut Pasteur, Paris, France.
authors.forename: David
authors.initials: D
authors.lastname: Bikard
authors.forename: Didier
authors.initials: D
authors.lastname: Mazel
mesh_terms: D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D032621:DNA Shuffling / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D019426:Integrases / Q000235:genetics; D032023:Integrons / Q000235:genetics*; D058615:Synthetic Biology / Q000379:methods*
chemical_list: D019426:Integrases; C115006:integron integrase IntI1