title: New chemodosimetric probe for the specific detection of Hg2+ in physiological condition and its utilisation for cell imaging studies.
abstract: A dithiane derivative of BODIPY is synthesized. This new reagent could be used for the specific and instantaneous detection of Hg(2+) in physiological condition. This dithiane reagent reacts specifically with Hg(2+) to regenerate the parent BODIPY-aldehyde with consequential change in visually detectable optical responses and this provides the possibility of using this reagent as a colorimetric probe or as a fluorescent biomarker/imaging reagent. Further, non-covalent interactions could be utilized for formation of an inclusion complex with biologically benign β-cyclodextrin for enhancing its solubility in aqueous environment and this included adduct could be used as a fluorescent marker and imaging reagent for Hg(2+). Uptake of Hg(2+) ions in live HeLa cells, exposed to a solution having Hg(2+) ion concentration as low as 2 ppb, could also be detected by confocal laser microscopic studies.
authors.affiliation: CSIR-Central Salt and Marine Chemical Research Institute, Bhavnagar 364002, Gujarat, India.
authors.forename: Sukdeb
authors.initials: S
authors.lastname: Saha
authors.forename: Hridesh
authors.initials: H
authors.lastname: Agarwalla
authors.forename: Hariom
authors.initials: H
authors.lastname: Gupta
authors.forename: Mithu
authors.initials: M
authors.lastname: Baidya
authors.forename: E
authors.initials: E
authors.lastname: Suresh
authors.forename: Sudip K
authors.initials: SK
authors.lastname: Ghosh
authors.forename: Amitava
authors.initials: A
authors.lastname: Das
mesh_terms: D001896:Boron Compounds / Q000737:chemistry; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008628:Mercury / Q000737:chemistry*; D057054:Molecular Imaging; D011807:Quinolizines / Q000737:chemistry; D013457:Sulfur Compounds / Q000737:chemistry
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D011807:Quinolizines; D013457:Sulfur Compounds; C438055:dithiane; D008628:Mercury