title: Regulation of TREM-1 expression by 1,25-dihydroxyvitamin D3 in human monocytes/macrophages.
abstract: Triggering receptor expressed on myeloid cells-1 (TREM-1) is a recently identified cell surface receptor that is expressed mainly on monocytes and neutrophils, and acts as an amplifier of immune responses. In this study, 1,25(OH)2D3 strongly upregulated the expression of TREM-1 in human monocytes and macrophages. 1,25(OH)2D3 stimulated TREM-1 mRNA expression by augmenting transcription, and not by inhibiting mRNA degradation. The upregulated expression of TREM-1 by 1,25(OH)2D3 was dependent on the NF-κB signaling pathway and required new protein synthesis in differentiated U937 macrophages. Our results show that 1,25(OH)2D3 can affect the innate and inflammatory responses by upregulating TREM-1 expression, and suggest that 1,25(OH)2D3 may function as an enhancer of the innate immune response by upregulating TREM-1 expression, in addition to inducing the antimicrobial peptide cathelicidin.
authors.affiliation: Hanyang University Hospital for Rheumatic Diseases, Seoul, Republic of Korea.
authors.forename: Tae-Hwan
authors.initials: TH
authors.lastname: Kim
authors.forename: Bitnara
authors.initials: B
authors.lastname: Lee
authors.forename: Eunji
authors.initials: E
authors.lastname: Kwon
authors.forename: Sung Jae
authors.initials: SJ
authors.lastname: Choi
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Lee
authors.forename: Gwan Gyu
authors.initials: GG
authors.lastname: Song
authors.forename: Jeongwon
authors.initials: J
authors.lastname: Sohn
authors.forename: Jong Dae
authors.initials: JD
authors.lastname: Ji
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000378:metabolism; D002117:Calcitriol / Q000494:pharmacology*; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D016328:NF-kappa B / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D015533:Transcriptional Activation / Q000187:drug effects; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D020298:U937 Cells; D015854:Up-Regulation / Q000187:drug effects; D054804:Cathelicidins
chemical_list: D000276:Adjuvants, Immunologic; D023181:Antimicrobial Cationic Peptides; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D012333:RNA, Messenger; D011971:Receptors, Immunologic; C408842:TREM1 protein, human; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D002117:Calcitriol; D054804:Cathelicidins
keywords: 1,25-Dihydroxyviamin D(3); Macrophages; Monocytes; TREM-1