title: Cell surface-specific N-glycan profiling in breast cancer.
abstract: Aberrant changes in specific glycans have been shown to be associated with immunosurveillance, tumorigenesis, tumor progression and metastasis. In this study, the N-glycan profiling of membrane proteins from human breast cancer cell lines and tissues was detected using modified DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE). The N-glycan profiles of membrane proteins were analyzed from 7 breast cancer cell lines and MCF 10A, as well as from 100 pairs of breast cancer and corresponding adjacent tissues. The results showed that, compared with the matched adjacent normal tissue samples, two biantennary N-glycans (NA2 and NA2FB) were significantly decreased (p <0.0001) in the breast cancer tissue samples, while the triantennary glycan (NA3FB) and a high-mannose glycan (M8) were dramatically increased (p = 0.001 and p <0.0001, respectively). Moreover, the alterations in these specific N-glycans occurred through the oncogenesis and progression of breast cancer. These results suggested that the modified method based on DSA-FACE is a high-throughput detection technology that is suited for analyzing cell surface N-glycans. These cell surface-specific N-glycans may be helpful in recognizing the mechanisms of tumor cell immunologic escape and could be potential targets for new breast cancer drugs.
authors.affiliation: School of Life Science and Technology, Harbin Institute of Technology, Harbin, Heilongjiang, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.forename: Huan
authors.initials: H
authors.lastname: Nie
authors.forename: Yubao
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yuanfei
authors.initials: Y
authors.lastname: Yao
authors.forename: Alaiyi
authors.initials: A
authors.lastname: Maitikabili
authors.forename: Youpeng
authors.initials: Y
authors.lastname: Qu
authors.forename: Shuliang
authors.initials: S
authors.lastname: Shi
authors.forename: Cuiying
authors.initials: C
authors.lastname: Chen
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D008875:Middle Aged; D009367:Neoplasm Staging; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D055815:Young Adult
chemical_list: D008565:Membrane Proteins; D011134:Polysaccharides