title: Iron-binding properties of sugar cane yeast peptides.
abstract: The extract of sugar-cane yeast (Saccharomyces cerevisiae) was enzymatically hydrolysed by Alcalase, Protex or Viscozyme. Hydrolysates were fractionated using a membrane ultrafiltration system and peptides smaller than 5kDa were evaluated for iron chelating ability through measurements of iron solubility, binding capacity and dialyzability. Iron-chelating peptides were isolated using immobilized metal affinity chromatography (IMAC). They showed higher content of His, Lys, and Arg than the original hydrolysates. In spite of poor iron solubility, hydrolysates of Viscozyme provided higher iron dialyzability than those of other enzymes. This means that more chelates of iron or complexes were formed and these kept the iron stable during simulated gastro-intestinal digestion in vitro, improving its dialyzability.
authors.affiliation: Instituto de Tecnologia de Alimentos, Food Chemistry and Applied Nutrition Center, Avenida Brasil, 2880, P.Box 139, Campinas, SP 13070-178,Brazil. lucia.delahoz@gmail.com
authors.forename: Lucia
authors.initials: L
authors.lastname: de la Hoz
authors.forename: Alexandre N
authors.initials: AN
authors.lastname: Ponezi
authors.forename: Raquel F
authors.initials: RF
authors.lastname: Milani
authors.forename: Vera S
authors.initials: VS
authors.lastname: Nunes da Silva
authors.forename: A
authors.initials: A
authors.lastname: Sonia de Souza
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Bertoldo-Pacheco
mesh_terms: D007501:Iron / Q000737:chemistry; D033862:Iron-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D011485:Protein Binding; D011492:Protein Hydrolysates / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000302:isolation & purification; D013381:Subtilisins / Q000737:chemistry
chemical_list: D033862:Iron-Binding Proteins; D010455:Peptides; D011492:Protein Hydrolysates; D029701:Saccharomyces cerevisiae Proteins; D007501:Iron; D013381:Subtilisins
keywords: Dialyzability; Immobilized metal affinity chromatography; Iron-chelating peptides; Protein hydrolysates; Sugar-cane yeast