title: Dose-related cerebellar abnormality in rats with prenatal exposure to X-irradiation by magnetic resonance imaging volumetric analysis.
abstract: Cerebellar abnormalities in 4-week-old rats with a single whole body X-irradiation at a dose of 0.5, 1.0, or 1.5 Gy on embryonic day (ED) 15 were examined by magnetic resonance imaging (MRI) volumetry. A 3D T2 W-MRI anatomical sequence with high-spatial resolution at 11.7-tesla was acquired from the fixed rat heads. By MRI volumetry, whole cerebellar volumes decreased dose-dependently. Multiple linear regression analysis revealed that the cortical volume (standardized β=0.901; P<0.001) was a major explanatory variable for the whole cerebellar volume, whereas both volumes of the white matter and deep cerebellar nuclei also decreased depending on the X-irradiation dose. The present MRI volumetric analysis revealed a dose-related cerebellar cortical hypoplasia by prenatal exposure to X-irradiation on E15.
authors.affiliation: Department of Physical Therapy, Faculty of Medical and Health Sciences, Tsukuba International University, Tsuchiura, Ibaraki, Japan. k-sawada@tius-hs.jp
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Sawada
authors.forename: Shigeyoshi
authors.initials: S
authors.lastname: Saito
authors.forename: Miwa
authors.initials: M
authors.lastname: Horiuchi-Hirose
authors.forename: Yuki
authors.initials: Y
authors.lastname: Mori
authors.forename: Yoshichika
authors.initials: Y
authors.lastname: Yoshioka
authors.forename: Kenya
authors.initials: K
authors.lastname: Murase
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000473:pathology; D002531:Cerebellum / Q000002:abnormalities* / Q000528:radiation effects; D002658:Developmental Disabilities / Q000175:diagnosis; D004307:Dose-Response Relationship, Radiation; D005260:Female; D008279:Magnetic Resonance Imaging; D018811:Maternal Exposure / Q000009:adverse effects*; D009421:Nervous System Malformations / Q000175:diagnosis*; D009929:Organ Size; D011247:Pregnancy; D051381:Rats; D014965:X-Rays / Q000009:adverse effects*
keywords: Purkinje cells; cerebellar hypoplasia; ionizing irradiation; magnetic resonance imaging; voxel-based morphometry