title: The study of absorption kinetics of berberine based on portal vein in rat, and the influence of verapamil and borneol to its absorption ability by UHPLC method.
abstract: This paper aims to investigate the portal-vein absorption kinetics of berberine in rat, and the influence of P-gp inhibitors such as verapamil and borneol, to its absorption ability. In the paper, a validated UHPLC method was established to determine the berberine in plasma, and the portal-vein absorption model was applied to conduct the pharmacokinetic study. Animals were divided into four groups as follow: group berberine group (BG); group verapamil + berberine group (VBG); group borneol + berberine group (BBG) and group long-term use of borneol + berberine group (LBBG). Plasma samples were obtained at regular time intervals after administration and separated on Agilent 1290 Infinity UHPLC instrument. The method showed good linearity (r = 0.9988) over wide dynamic ranges (4.08-163.20 ng/mL). Variations within- and between-batch never exceeded 7.58 and 2.21 %, respectively. The extraction recovery rates ranged from 85.34 to 111.62 %. We discovered that the AUC of four group exhibited no significant differences (P > 0.05), and co-administration of berberine with borneol of group BBG and group LBBG in advance could both reduce the T max and C max as compared to group BG (P < 0.05), while co-administration of verapamil seems to have had little influence to berberine's absorption ability.
authors.affiliation: College of Biological Industry, Chengdu University, Chengdu, 610106, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zou
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiao
authors.forename: Li-Jia
authors.initials: LJ
authors.lastname: Xu
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: He
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.forename: Lian-Xin
authors.initials: LX
authors.lastname: Peng
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D001599:Berberine / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D001682:Biological Availability; D002138:Calibration; D001892:Camphanes / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D004347:Drug Interactions; D005260:Female; D065569:Gastrointestinal Absorption / Q000187:drug effects*; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D008954:Models, Biological; D011169:Portal Vein; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D014700:Verapamil / Q000494:pharmacology*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D001892:Camphanes; D001599:Berberine; D014700:Verapamil; C022871:isoborneol