title: Bisphenol A modifies the regulation exerted by testosterone on 5 α -reductase isozymes in ventral prostate of adult rats.
abstract: The development, growth, and function of the prostate gland depend on androgen stimulation. The primary androgen in prostate is 5α-dihydrotestosterone (DHT) which is synthesized from circulating testosterone (T) through the action of 5α-reductase (5α-R). Although 5α-R occurs as five isozymes, only 5α-R1 and 5α-R2 are physiologically involved in steroidogenesis. The endocrine disruptor bisphenol A (BPA) alters sexual organs, including the prostate. Our previous findings indicated that BPA decreased the expression of 5α-R1 and 5α-R2 in rat prostate but also circulating T. Thus, it is unclear whether BPA exerts this effect on 5α-R isozymes by reducing circulating T or by any other mechanism. In this study, we examine the effects of short-term exposure to BPA at doses below 25 μg/Kg/d and above 300 μg/Kg/d of the TDI on mRNA levels of 5α-R1 and 5α-R2 in prostate of adult castrated rats supplemented with T to achieve constant circulating T levels. mRNA levels were measured by absolute quantitative RT-PCR, T levels by RIA, and DHT levels by ELISA. Our results indicated that in castrated rats treated with T BPA at the two doses studied significantly decreased the mRNA levels of both 5α-R isozymes in a dose-dependent manner without modifications in circulating T.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, Avenida de Madrid, 18012 Granada, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Sánchez
authors.forename: Beatriz
authors.initials: B
authors.lastname: Castro
authors.forename: Jesús M
authors.initials: JM
authors.lastname: Torres
authors.forename: Asunción
authors.initials: A
authors.lastname: Olmo
authors.forename: Raimundo G
authors.initials: RG
authors.lastname: del Moral
authors.forename: Esperanza
authors.initials: E
authors.lastname: Ortega
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D002369:Castration; D042944:Cholestenone 5 alpha-Reductase / Q000235:genetics / Q000378:metabolism*; D013196:Dihydrotestosterone / Q000097:blood; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010636:Phenols / Q000633:toxicity*; D011467:Prostate / Q000187:drug effects* / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013739:Testosterone / Q000097:blood / Q000494:pharmacology*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D042944:Cholestenone 5 alpha-Reductase; D013196:Dihydrotestosterone; D007527:Isoenzymes; D010636:Phenols; D012333:RNA, Messenger; D013739:Testosterone; C006780:bisphenol A; D000098826:Bisphenol A Compounds