title: Detention of HPV L1 Capsid Protein and hTERC Gene in Screening of Cervical Cancer.
abstract: OBJECTIVE
  To investigate the expression of human papilloma virus (HPV) L1 capsid protein, and human telomerase RNA component (hTERC) in cervical cancer and the role of detection of both genes in screening of cervical cancer.
  
  
  METHODS
  A total of 309 patients were recruited and cervical exfoliated cells were collected. Immunocytochemistry was employed to detect HPV L1 capsid protein, and fluorescent in situ hybridization (FISH) was performed to detect the hTERC.
  
  
  RESULTS
  The expression of HPV L1 capsid protein reduced with the increase of the histological grade of cervical cells and was negatively related to the grade of cervical lesions. However, the expression of hTERC increased with the increase of the histological grade and positively associated with the grade of cervical lesions. The proportion of patients with L1(-)/hTERC(+) was higher in patients with histological grade of CIN2 or higher than that in those with histological grade of CIN1. The L1(+)/hTERC(-) and L1(-)/hTERC(-) were negatively related to the grade of cervical lesions. L1(-)/hTERC(+) was positively associated with the grade of cervical lesions. The L1/hTERC ratio increased. The negative predictive value of both HPV L1 and hTERC was higher than that of HPV L1 or hTERC, but there was no marked difference in the screening efficacy of cervical cancer among HPV L1, hTERC and HPV L1+hTERC.
  
  
  CONCLUSIONS
  HPV L1 capsid protein and hTERC gene may serve as markers for the early diagnosis and prediction of cervical lesions. The increase in L1/hTERC ratio reflects the progression of cervical lesions to a certain extent.
authors.affiliation: Department of Gynecology and Obstetrics, Shenzhen Hospital of Beijing University, Shenzhen 518036, China.
authors.forename: Huang
authors.initials: H
authors.lastname: Bin
authors.forename: Wu
authors.initials: W
authors.lastname: Ruifang
authors.forename: Li
authors.initials: L
authors.lastname: Ruizhen
authors.forename: Liang
authors.initials: L
authors.lastname: Yiheng
authors.forename: Liu
authors.initials: L
authors.lastname: Zhihong
authors.forename: Li
authors.initials: L
authors.lastname: Juan
authors.forename: Wang
authors.initials: W
authors.lastname: Chun
authors.forename: Zhou
authors.initials: Z
authors.lastname: Yanqiu
authors.forename: Weng
authors.initials: W
authors.lastname: Leiming
keywords: Cervical lesion; Human papilloma virus; Human telomerase RNA component; L1 capsid protein; Squamous cell carcinomas