title: Bioactive components and preventive effect of green tea (Camellia sinensis) extract on post-laparotomy intra-abdominal adhesion in rats.
abstract: BACKGROUND
  Adhesion formation is an important complication of abdomino-pelvic surgery. Green tea (Camellia sinensis) has anti-oxidant and anti-inflammatory effects which prevent production and accumulation of collagen and, thus, may reduce adhesion formation. The present study examined the effect of green tea alcoholic extract on intra-abdominal adhesion formation. Total phenolic, flavonoid and flavonol contents as well as anti-oxidant activity were also evaluated.
  
  
  METHODS
  Thirty healthy male Wistar rats were randomly assigned to two equal groups of green tea (A) and distilled water (B). After anesthesia, the abdominal wall was opened and three shallow longitudinal and transverse incisions of 2 cm in length were made on the right side of the peritoneum by scalpel blade. A 2 × 2 cm square of the left abdominal wall peritoneum was removed by surgical scissors. Green tea extract or distilled water was introduced into the abdominal cavity of each rat. The rats were sacrificed two weeks post-laparotomy and adhesion bands were scored according to severity, extent and appearance. Fibrosis and inflammation were also scored via histopathological examination.
  
  
  RESULTS
  There was a significant difference in mean adhesion scores between the green tea and distilled water groups (3.2 ± 3.503 and 7.33 ± 0.51, respectively) (p = 0.001). In terms of fibrosis (p = 0.002) and inflammation (p = 0.003) a statistically significant difference was also seen between the two groups following histopathological examination.
  
  
  CONCLUSIONS
  Green tea extract reduces intra-peritoneal adhesions in an animal model.
authors.affiliation: Member of Young Researchers Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran. Electronic address: pouyaparsaei@yahoo.com.
authors.forename: Pouya
authors.initials: P
authors.lastname: Parsaei
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Karimi
authors.forename: Sayyed Yazdan
authors.initials: SY
authors.lastname: Asadi
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Rafieian-Kopaei
mesh_terms: D034841:Abdominal Cavity / Q000473:pathology / Q000601:surgery; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D028241:Camellia sinensis / Q000737:chemistry*; D007813:Laparotomy / Q000009:adverse effects / Q000379:methods*; D008297:Male; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D000267:Tissue Adhesions / Q000188:drug therapy* / Q000473:pathology / Q000517:prevention & control*
chemical_list: D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts
keywords: Green tea; Intra-abdominal adhesions; Laparotomy; Rat