title: A humidity-sensitive hydrogel-Bacillus spore composite for micropatterning of biomolecular gradients.
abstract: A composite material consisting of Bacillus subtilis spores suspended in a humidity sensitive hydrogel can be used to pattern biomolecules in different concentrations directly onto glass surfaces using a mechanical micromanipulator. By altering the relative humidity surrounding the composite gel during deposition, surface concentration of patterned biomolecules can be controlled and varied to create user-defined, biomolecular surface concentrations.
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: Richard L
authors.initials: RL
authors.lastname: Gieseck
authors.forename: Bin-Da
authors.initials: BD
authors.lastname: Chan
authors.forename: Cagri A
authors.initials: CA
authors.lastname: Savran
mesh_terms: D001412:Bacillus subtilis; D005898:Glass / Q000737:chemistry; D006813:Humidity; D020100:Hydrogels / Q000737:chemistry*; D055616:Microtechnology / Q000295:instrumentation*; D013171:Spores, Bacterial; D013499:Surface Properties
chemical_list: D020100:Hydrogels