title: Merging a sensitive capillary electrophoresis-ultraviolet detection method with chemometric exploratory data analysis for the determination of phenolic acids and subsequent characterization of avocado fruit.
abstract: Herein we present the development of a powerful CE-UV method able to detect and quantify an important number of phenolic acids in 13 varieties of avocado fruits at 2 ripening stages. All the variables involved in CE separation were exhaustively optimized and the best results were obtained with a capillary of 50 μm i.d. × 50 cm effective length, sodium tetraborate 40 mM at a pH of 9.4, 30 kV, 25 °C, 10s of hydrodynamic injection (0.5 psi) and UV detection at 254 nm. This optimal methodology was fully validated and then applied to different avocado samples. The number of phenolic acids determined varied from 8 to 14 compounds; in general, they were in concentrations ranging from 0.13 ppm to 3.82 ppm, except p-coumaric, benzoic and protocatechuic acids, which were found at higher concentrations. Principal component analysis (PCA) was applied to highlight the differences between varieties and ripening degrees, looking for the most influential analytes.
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Ave. Fuentenueva s/n, E-18071 Granada, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Hurtado-Fernández
authors.forename: Paulina K
authors.initials: PK
authors.lastname: Contreras-Gutiérrez
authors.forename: Luis
authors.initials: L
authors.lastname: Cuadros-Rodríguez
authors.forename: Alegría
authors.initials: A
authors.lastname: Carrasco-Pancorbo
authors.forename: Alberto
authors.initials: A
authors.lastname: Fernández-Gutiérrez
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D062385:Hydroxybenzoates / Q000032:analysis*; D027421:Persea / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D062385:Hydroxybenzoates; C017616:phenolic acid
keywords: Avocado; Capillary electrophoresis; Persea americana; Phenolic acids; Principal component analysis