title: Genomic study of polyhydroxyalkanoates producing Aeromonas hydrophila 4AK4.
abstract: The complete genome of Gram-negative Aeromonas hydrophila 4AK4 that has been used for industrial production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) was sequenced and annotated. Its chromosome is 4,527,993 bp in size encoding 4,272 genes, including 28 rRNA genes and 104 tRNA genes. Comparative analysis indicated that genome of A. hydrophila 4AK4 was similar to that of the A. hydrophila ATCC 7966(T), an intensively studied aeromonad for its pathogenicity related to its genomic information. Genes possibly coming from other species or even other genus were identified in A. hydrophila 4AK4. A large number of putative virulent genes were predicted. However, a cytotonic enterotoxin (Ast) is absent in A. hydrophila 4AK4, allowing the industrial strain to be different from other A. hydrophila strains, indicating possible reduced virulence of strain 4AK4, which is very important for industrial fermentation. Genes involved in polyhydroxyalkanoate (PHA) metabolism were predicted and analyzed. The resulting genomic information is useful for improved production of PHA via metabolic engineering of A. hydrophila 4AK4.
authors.affiliation: MOE Key Lab of Bioinformatics, School of Life Science, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, 100084, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Gao
authors.forename: Jia
authors.initials: J
authors.lastname: Jian
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Li
authors.forename: Yu-Cheng
authors.initials: YC
authors.lastname: Yang
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Shen
authors.forename: Zhi-Rong
authors.initials: ZR
authors.lastname: Sun
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Chen
mesh_terms: D016980:Aeromonas hydrophila / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D016680:Genome, Bacterial; D023281:Genomics; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D054813:Polyhydroxyalkanoates