title: Neuropharmcological potential of methanolic extract and a triterpene isolated from Madhuca longifolia L leaves in mice.
abstract: The methanolic extract of M. longifolia (MLME) and a compound a triterpene, derivative of madhucic acid (dMA) isolated from the leaves of M. longifolia, were investigated for their possible neuropharmacological activities in mice using phenobarbitone induced sleeping time, spontaneous motor activity, marble burying test and Eddy's hot plate method. LD50 for MLME and dMA were 100 and 10 mg/kg of body weight, respectively. Both MLME and dMA (10 mg/kg and 2 mg/kg oral route respectively) exhibited significant increase in phenobarbitone induced sleeping time, greater reduction in spontaneous motor activity and marble burying activity, confirming their sedative nature. Both MLME and dMA also exhibited considerable antinociceptive activity in experimental animals. The results suggest that both MLME and dMA have CNS depressant activity in mice.
authors.affiliation: Department of Biochemistry, Gulbarga University, Gulbarga 585106, India.
authors.forename: Triveni S
authors.initials: TS
authors.lastname: Inganakal
authors.forename: Md Liyakhat
authors.initials: ML
authors.lastname: Ahmed
authors.forename: Paramjyothi
authors.initials: P
authors.lastname: Swamy
mesh_terms: D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D002492:Central Nervous System Depressants / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D003192:Compulsive Behavior / Q000188:drug therapy; D004353:Drug Evaluation, Preclinical; D006358:Hot Temperature / Q000009:adverse effects; D006993:Hypnotics and Sedatives / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D007928:Lethal Dose 50; D032128:Madhuca / Q000737:chemistry*; D008297:Male; D000432:Methanol; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D059225:Nociception / Q000187:drug effects; D010634:Phenobarbital / Q000494:pharmacology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry; D012890:Sleep / Q000187:drug effects; D012997:Solvents; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000700:Analgesics; D002492:Central Nervous System Depressants; D006993:Hypnotics and Sedatives; D010936:Plant Extracts; D012997:Solvents; D014315:Triterpenes; D000432:Methanol; D010634:Phenobarbital