title: Expression of the 1-SST and 1-FFT genes and consequent fructan accumulation in Agave tequilana and A. inaequidens is differentially induced by diverse (a)biotic-stress related elicitors.
abstract: The expression of genes coding for sucrose:sucrose 1-fructosyltransferase (1-SST; EC 2.4.1.99) and fructan:fructan 1-fructosyltransferase (1-FFT; EC 2.4.1.100), both fructan biosynthesizing enzymes, characterization by TLC and HPAEC-PAD, as well as the quantification of the fructo-oligosaccharides (FOS) accumulating in response to the exogenous application of sucrose, kinetin (cytokinin) or other plant hormones associated with (a)biotic stress responses were determined in two Agave species grown in vitro, domesticated Agave tequilana var. azul and wild A. inaequidens. It was found that elicitors such as salicylic acid (SA), and jasmonic acid methyl ester (MeJA) had the strongest effect on fructo-oligosaccharide (FOS) accumulation. The exogenous application of 1mM SA induced a 36-fold accumulation of FOS of various degrees of polymerization (DP) in stems of A. tequilana. Other treatments, such as 50mM abscisic acid (ABA), 8% Sucrose (Suc), and 1.0 mg L(-1) kinetin (KIN) also led to a significant accumulation of low and high DP FOS in this species. Conversely, treatment with 200 μM MeJA, which was toxic to A. tequilana, induced an 85-fold accumulation of FOS in the stems of A. inaequidens. Significant FOS accumulation in this species also occurred in response to treatments with 1mM SA, 8% Suc, and 10% polyethylene glycol (PEG). Maximum yields of 13.6 and 8.9 mg FOS per g FW were obtained in stems of A. tequilana and A. inaequidens, respectively. FOS accumulation in the above treatments was tightly associated with increased expression levels of either the 1-FFT or the 1-SST gene in tissues of both Agave species.
authors.affiliation: Laboratorio de Biología Molecular, Instituto Tecnológico de Tlajomulco, Jalisco (ITTJ), km 10 Carretera a San Miguel Cuyutlán, CP 45640 Tlajomulco de Zúñiga, Jalisco, Mexico.
authors.forename: Edgar Martín
authors.initials: EM
authors.lastname: Suárez-González
authors.affiliation: Unidad de Biotecnología e Ingeniería Genética de Plantas, Cinvestav-Unidad Irapuato, km 9.6, Libramiento Norte Carretera Irapuato-León, CP 36821 Irapuato, Guanajuato, Mexico.
authors.forename: Mercedes G
authors.initials: MG
authors.lastname: López
authors.affiliation: Unidad de Biotecnología e Ingeniería Genética de Plantas, Cinvestav-Unidad Irapuato, km 9.6, Libramiento Norte Carretera Irapuato-León, CP 36821 Irapuato, Guanajuato, Mexico.
authors.forename: John P
authors.initials: JP
authors.lastname: Délano-Frier
authors.affiliation: Laboratorio de Biología Molecular, Instituto Tecnológico de Tlajomulco, Jalisco (ITTJ), km 10 Carretera a San Miguel Cuyutlán, CP 45640 Tlajomulco de Zúñiga, Jalisco, Mexico. Electronic address: jfleyva@hotmail.com.
authors.forename: Juan Florencio
authors.initials: JF
authors.lastname: Gómez-Leyva
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000085:Acetates / Q000494:pharmacology; D027882:Agave / Q000187:drug effects / Q000378:metabolism*; D003517:Cyclopentanes / Q000494:pharmacology; D005630:Fructans / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D054883:Oxylipins / Q000494:pharmacology; D010940:Plant Proteins / Q000378:metabolism*; D020156:Salicylic Acid / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D005630:Fructans; D054883:Oxylipins; D010940:Plant Proteins; D013395:Sucrose; D000040:Abscisic Acid; C072239:methyl jasmonate; D020156:Salicylic Acid
keywords: Abiotic stress; Agave sp.; Biotic stress; Fructo-oligosaccharides; Fructosyltransferases