title: Relative effect of bioaugmentation with electrochemically active and non-active bacteria on bioelectrogenesis in microbial fuel cell.
abstract: Bioelectrogenic activity of microbial fuel cells (MFC) augmented with electrochemically active bacteria (EAB, Pseudomonas aeruginosa) and non-EAB (Escherichia coli) as biocatalysts was investigated. Anodic microflora augmented with P. aeruginosa (AMFCP) yielded higher electrogenic activity (418 mV; 3.87 mA) than E. coli (AMFCE; 254 mV; 1.67 mA) and non-augmented native microflora (MFCC; 235 mV; 1.37 mA). Higher redox currents along with lower Tafel-slopes were observed with AMFCP operation compared to AMFCE and MFCC due to manifestation of bioaugmentation thereby minimizing the losses. A fourfold and twofold increase in capacitance and exchange current was observed with AMFCP and AMFCE operation respectively, when compared to MFCC. Tracking of augmented biocatalyst by fluorescent in situ hybridization (FISH) with defined probes documented the survivability of Pseudomonas sp. in higher numbers than Enterobacteriaceae. Study corroborated enhanced electron transfer capability of mixed consortia owing to the synergistic interaction with EAB due to augmentation.
authors.affiliation: Bioengineering and Environmental Centre (BEEC), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India.
authors.forename: S Veer
authors.initials: SV
authors.lastname: Raghavulu
authors.affiliation: Bioengineering and Environmental Centre (BEEC), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India.
authors.forename: J Annie
authors.initials: JA
authors.lastname: Modestra
authors.affiliation: Bioengineering and Environmental Centre (BEEC), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India.
authors.forename: K
authors.initials: K
authors.lastname: Amulya
authors.affiliation: Bioengineering and Environmental Centre (BEEC), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India.
authors.forename: C Nagendranatha
authors.initials: CN
authors.lastname: Reddy
authors.affiliation: Bioengineering and Environmental Centre (BEEC), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India. Electronic address: vmohan_s@yahoo.com.
authors.forename: S
authors.initials: S
authors.lastname: Venkata Mohan
mesh_terms: D001673:Biodegradation, Environmental; D001674:Bioelectric Energy Sources; D057919:Biological Oxygen Demand Analysis; D002384:Catalysis; D004563:Electrochemistry / Q000379:methods*; D004583:Electrons; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D017404:In Situ Hybridization, Fluorescence; D059013:Microbial Consortia; D010084:Oxidation-Reduction; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*; D053038:Quorum Sensing; D013997:Time Factors; D062065:Wastewater; D014874:Water Pollutants, Chemical / Q000032:analysis; D018508:Water Purification / Q000379:methods
chemical_list: D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Bioelectricity; Electron transfer; Mediators; Quorum sensing; Wastewater