title: Release-active dilutions of diclofenac enhance anti-inflammatory effect of diclofenac in carrageenan-induced rat paw edema model.
abstract: The study was aimed to investigate the effect of technologically treated diclofenac (release-active dilutions of diclofenac (RAD of diclofenac)) on anti-inflammatory activity of diclofenac in carrageenan-induced rat paw edema model. Ninety male Wistar albino rats (6-8 weeks) divided into nine groups (n = 10) were used. Anti-inflammatory activity was assessed at 1, 2, 3, 4, and 6 h after subplantar injection of carrageenan (0.1 ml of a 1 % solution in normal saline). Diclofenac alone was studied at 5 and 20 mg/kg, RAD of diclofenac alone at 7.5 ml/kg and their combination at 5 and 7.5 ml/kg, respectively. Diclofenac reduced (p < 0.05 at least) paw edema at all time points. RAD of diclofenac enhanced (p < 0.05) anti-inflammatory effect of diclofenac (5 mg/kg) at 2, 4, and 6 h on concurrent and at 2 and 4 h on sequential administration. Moreover at 2 h, anti-inflammatory effect of combination treatment reached values comparable to those of diclofenac (20 mg/kg). In conclusion, RAD of diclofenac enhanced anti-inflammatory effect of diclofenac.
authors.affiliation: Dabur Research Foundation, 22, Site IV, Sahibabad, 201-010, Ghaziabad, Uttar Pradesh, India, dr.sachin.sakat@daburresearch.in.
authors.forename: Sachin S
authors.initials: SS
authors.lastname: Sakat
authors.forename: Kamaraj
authors.initials: K
authors.lastname: Mani
authors.forename: Yulia O
authors.initials: YO
authors.lastname: Demidchenko
authors.forename: Evgeniy A
authors.initials: EA
authors.lastname: Gorbunov
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Tarasov
authors.forename: Archna
authors.initials: A
authors.lastname: Mathur
authors.forename: Oleg I
authors.initials: OI
authors.lastname: Epstein
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D002351:Carrageenan; D016861:Cyclooxygenase Inhibitors / Q000627:therapeutic use; D004008:Diclofenac / Q000031:analogs & derivatives / Q000627:therapeutic use*; D004487:Edema / Q000188:drug therapy*; D005528:Foot / Q000473:pathology; D007249:Inflammation / Q000188:drug therapy*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016861:Cyclooxygenase Inhibitors; D004008:Diclofenac; D002351:Carrageenan