title: From invisible structures of SWCNTs toward fluorescent and targeting architectures for cell imaging.
abstract: Single-walled carbon nanotubes (SWNTs) are unique nanostructures used as cargo systems for variety of diagnostic and therapeutic agents. For taking advantage of these structures in biological processes, they should be visible. Therefore, fluorescence labeling of SWCNTs with various probes is a significant issue. Herein, we demonstrate a simple approach for cell specific imaging and diagnosis by combining SWCNTs with a copolymer poly(para-phenylene) (PPP) containing polystyrene (PSt) and poly(ε-caprolactone) (PCL) side chains (PPP-g-PSt-PCL). In this approach PPP-g-PSt-PCL is noncovalently attached on carboxyl functional SWCNTs. The obtained fluorescent probe is bound to folic acid (FA) for targeted imaging of folate receptor (FR) positive HeLa cells. In vitro studies demonstrate that this conjugate can specifically bind to HeLa cells and indicate great potential for targeting and imaging studies.
authors.affiliation: Department of Biochemistry, Faculty of Science, Ege University , 35100 Bornova-Izmir, Turkey.
authors.forename: Didem
authors.initials: D
authors.lastname: Ag
authors.forename: Muharrem
authors.initials: M
authors.lastname: Seleci
authors.forename: Rebecca
authors.initials: R
authors.lastname: Bongartz
authors.forename: Mustafa
authors.initials: M
authors.lastname: Can
authors.forename: Seda
authors.initials: S
authors.lastname: Yurteri
authors.forename: Ioan
authors.initials: I
authors.lastname: Cianga
authors.forename: Frank
authors.initials: F
authors.lastname: Stahl
authors.forename: Suna
authors.initials: S
authors.lastname: Timur
authors.forename: Thomas
authors.initials: T
authors.lastname: Scheper
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Yagci
mesh_terms: D045744:Cell Line, Tumor; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry; D005492:Folic Acid / Q000737:chemistry; D058972:Folic Acid Transporters / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D057054:Molecular Imaging; D015394:Molecular Structure; D037742:Nanotubes, Carbon / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D058972:Folic Acid Transporters; D037742:Nanotubes, Carbon; D005492:Folic Acid