title: Novel mastadenovirus infection and clinical disease in a pygmy marmoset (Callithrix [Cebuella] pygmaea).
abstract: We describe the detection and successful isolation of a novel mastadenovirus from a pygmy marmoset (Callithrix [Cebuella] pygmaea) that died following an episode of severe respiratory signs. Pathologic/histopathologic examination revealed hydrothorax and catarrhal bronchopneumonia with pronounced desquamation of the bronchiolar epithelial cells, while in other airways a marked hyperplasia of the epithelial lining and numerous giant cells could be observed. We obtained partial sequence data from the adenoviral DNA-dependent DNA-polymerase gene of the isolated strain and analyses of this region showed the highest level of identity to the recently described bat adenoviruses (strains PPV1 and TJM) and the type 2 canine adenovirus. Similar results were gained by phylogenetic calculations indicating that this novel marmoset adenovirus is only distantly related to reference Old and New World primate adenoviruses and formed a monophyletic group with bat and canine adenoviruses and the equine adenovirus 1. Even though the source of the infection remained unknown, our results could imply the possibility of a cross-species transmission of the virus from an anonymous host to the pygmy marmoset.
authors.affiliation: Department of Pathology and Forensic Veterinary Medicine, Division of Exotic and Wild Animal Medicine, Szent István University, Faculty of Veterinary Science, István u. 2, H-1078 Budapest, Hungary.
authors.forename: János
authors.initials: J
authors.lastname: Gál
authors.forename: Ákos
authors.initials: Á
authors.lastname: Hornyák
authors.forename: Míra
authors.initials: M
authors.lastname: Mándoki
authors.forename: Tamás
authors.initials: T
authors.lastname: Bakonyi
authors.forename: Gyula
authors.initials: G
authors.lastname: Balka
authors.forename: Levente
authors.initials: L
authors.lastname: Szeredi
authors.forename: Miklós
authors.initials: M
authors.lastname: Marosán
authors.forename: Tibor
authors.initials: T
authors.lastname: Ludányi
authors.forename: Petra
authors.initials: P
authors.lastname: Forgách
authors.forename: Endre
authors.initials: E
authors.lastname: Sós
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Demeter
authors.forename: Szilvia L
authors.initials: SL
authors.lastname: Farkas
mesh_terms: D000257:Adenoviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D000818:Animals; D000836:Animals, Zoo / Q000821:virology; D002144:Callithrix / Q000821:virology*; D002417:Cattle; D002460:Cell Line; D002522:Chlorocebus aethiops; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D006814:Hungary; D008297:Male; D017921:Mastadenovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D008992:Monkey Diseases / Q000473:pathology / Q000821:virology*; D010802:Phylogeny; D014709:Vero Cells
chemical_list: D004259:DNA-Directed DNA Polymerase
keywords: Adenoviridae; Callithrix (Cebuella) pygmaea; Mastadenovirus; Pygmy marmoset