title: Impacts of light and temperature on shoot branching gradient and expression of strigolactone synthesis and signalling genes in rose.
abstract: Light and temperature are two environmental factors that deeply affect bud outgrowth. However, little is known about their impact on the bud burst gradient along a stem and their interactions with the molecular mechanisms of bud burst control. We investigated this question in two acrotonic rose cultivars. We demonstrated that the darkening of distal buds or exposure to cold (5 °C) prior to transfer to mild temperatures (20 °C) both repress acrotony, allowing the burst of quiescent medial and proximal buds. We sequenced the strigolactone pathway MAX-homologous genes in rose and studied their expression in buds and internodes along the stem. Only expressions of RwMAX1, RwMAX2 and RwMAX4 were detected. Darkening of the distal part of the shoot triggered a strong increase of RwMAX2 expression in darkened buds and bark-phloem samples, whereas it suppressed the acropetal gradient of the expression of RwMAX1 observed in stems fully exposed to light. Cold treatment induced an acropetal gradient of expression of RwMAX1 in internodes and of RwMAX2 in buds along the stem. Our results suggest that the bud burst gradient along the stem cannot be explained by a gradient of expression of RwMAX genes but rather by their local level of expression at each individual position.
authors.affiliation: Université d'Angers, UMR1345 Institut de Recherche en Horticulture et Semences, SFR 4207 QUASAV, PRES L'UNAM, F-49045, Angers, France; INRA, UMR1345 Institut de Recherche en Horticulture et Semences, F-49071, Beaucouzé, France.
authors.forename: Samia
authors.initials: S
authors.lastname: Djennane
authors.forename: Laurence
authors.initials: L
authors.lastname: Hibrand-Saint Oyant
authors.forename: Koji
authors.initials: K
authors.lastname: Kawamura
authors.forename: David
authors.initials: D
authors.lastname: Lalanne
authors.forename: Michel
authors.initials: M
authors.lastname: Laffaire
authors.forename: Tatiana
authors.initials: T
authors.lastname: Thouroude
authors.forename: Séverine
authors.initials: S
authors.lastname: Chalain
authors.forename: Soulaiman
authors.initials: S
authors.lastname: Sakr
authors.forename: Rachid
authors.initials: R
authors.lastname: Boumaza
authors.forename: Fabrice
authors.initials: F
authors.lastname: Foucher
authors.forename: Nathalie
authors.initials: N
authors.lastname: Leduc
mesh_terms: D002874:Chromosome Mapping; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D017343:Genes, Plant / Q000235:genetics; D007783:Lactones / Q000378:metabolism*; D008027:Light; D010802:Phylogeny; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development* / Q000528:radiation effects; D040641:Quantitative Trait Loci / Q000235:genetics; D027826:Rosa / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology / Q000528:radiation effects; D015398:Signal Transduction / Q000235:genetics* / Q000528:radiation effects; D013696:Temperature
chemical_list: D007783:Lactones
keywords: MAX genes; Rosa; axillary bud; bud burst; environmental factors; growth regulators; hormones; plant architecture