title: [Expression level of Th22 cells and its cytokines in patients with acute lymphoblastic leukemia and its significance].
abstract: This study was purposed to analyze the expression level of Th22 cells and their cytokines in patients with acute lymphoblastic leukemia (ALL) and evaluate its significance. Forty-eight patients with ALL were selected. According to the treatment, all patients were divided into the newly diagnosed group (n = 26) and complete remission (CR) group (n = 22). The proportion of Th22 cells in peripheral blood was detected by flow cytometry (FCM). The expression levels of cytokines IL-22, IL-6, TNF-α and TGF-β in peripheral blood were measured by ELISA. The expression level of IL-22 mRNA in peripheral blood mononuclear cells was examined by semi-quantitative-reverse transcription PCR (RT-PCR). Meanwhile, 30 healthy individuals were selected as a control group. The parameters of the 3 groups were compared. The results showed that the percentage of Th22 cells and the expression levels of IL-22, IL-6, TNF-α and IL-22 mRNA in newly diagnosed group and the CR group were significantly lower than that in control group, the expression level of TGF-β in above mentioned two group was obviously higher than that in control group (P < 0.05). The percentage of Th22 cells and the expression levels of IL-22, IL-6, TNF-α and IL-22 mRNA in newly diagnosed group were evidently lower than that in CR group (P < 0.05), but the expression level of TGF-β in newly diagnosed group obviously higher than that in CR group. The expression level of IL-22 in newly diagnosed group was positively related with expression level of IL-6 and TNF-α, but it was negatively related with expression level of TGF-β. It is concluded that the decreasing of Th22 cells and down-regulation of IL-22 expression level may be related with pathogenesis of ALL, the decreasing of Th22 cells is risk factor for ALL.
authors.affiliation: Department of Hematology, Zhengzhou People's Hospital, Zhengzhou 450003, Henan Province, China (E-mail:chenronghua4you@qq.com).
authors.forename: Rong-Hua
authors.initials: RH
authors.lastname: Cheng
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D007378:Interleukins / Q000097:blood*; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D012074:Remission Induction; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D055815:Young Adult; D000097043:Interleukin-22
chemical_list: D015850:Interleukin-6; D007378:Interleukins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D000097043:Interleukin-22