title: Cell surface markers for T and B lymphocytes activation and adhesion as putative prognostic biomarkers for head and neck squamous cell carcinoma.
abstract: The study population comprised HNSCC patients, risk-positive controls (tabagism and alcoholism habits), and risk-negative controls (without risk factors). Significant increases in the activation status of CD4(+)and CD8(+) T-cells, and higher migration potentials of lymphocytes were observed in HNSCC patients compared with control groups. Although decreased frequency of CD19(+)-B lymphocytes was observed in HSNCC patients, a higher percentage of HLA-DR(+)CD19(+)-B lymphocytes was detected in these individuals as compared with other evaluated groups. Metastasis and tumor grading were the major pathological parameters associated with significant alterations in the expression of activation molecules on circulating CD4(+) and CD8(+) T-cells. A reduced frequency of CD38-expressing CD8(+) T-cells was the most relevant biomarker associated with HNSCC aggressiveness. Performance analysis suggested a cut-off point for the CD8(+)CD38(+)/CD8(+) T-cell ratio of 7.0 for segregating patients according to tumor grading. In contrast, a higher proportion of CD8(+)CD54(+)/CD8(+) T-cells could represent a relevant biomarker associated with metastasis in HNSCC patients, and performance analysis suggested a cut-off point for the CD8(+)CD54(+)/CD8(+) T-cell ratio of 30 for segregating patients according to absence or presence of metastasis. The results obtained can increment immunological aspects of HNSCC and provide tools for the determination of cut-off scores of clinically relevant immunophenotypic prognostic biomarkers.
authors.affiliation: Universidade Estadual de Montes Claros (UNIMONTES), 39401-089 Montes Claros, MG, Brazil. Electronic address: marileia@cpqrr.fiocruz.br.
authors.forename: Mariléia Chaves
authors.initials: MC
authors.lastname: Andrade
authors.forename: Shirlene Barbosa Pimentel
authors.initials: SB
authors.lastname: Ferreira
authors.forename: Luciana C
authors.initials: LC
authors.lastname: Gonçalves
authors.forename: Alfredo Maurício Batista
authors.initials: AM
authors.lastname: De-Paula
authors.forename: Elaine Speziali
authors.initials: ES
authors.lastname: de Faria
authors.forename: Andréa
authors.initials: A
authors.lastname: Teixeira-Carvalho
authors.forename: Olindo Assis
authors.initials: OA
authors.lastname: Martins-Filho
mesh_terms: D000328:Adult; D000368:Aged; D000954:Antigens, Surface; D016175:B-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002465:Cell Movement; D003430:Cross-Sectional Studies; D018450:Disease Progression; D005260:Female; D006258:Head and Neck Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D016130:Immunophenotyping; D008213:Lymphocyte Activation / Q000276:immunology*; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D012307:Risk Factors; D000077195:Squamous Cell Carcinoma of Head and Neck; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism*
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers; D015815:Cell Adhesion Molecules
keywords: AUC; BD; Becton–Dickinson; CD; EDTA; FITC; FSC; HIV; HLA; HNSCC; International Union Against Cancer; LR; NPV; PE; PPV; ROC; SSC; TC; TNM; UICC; WHO; cluster of differentiation; ethylenediaminetetraacetic acid; fluorescein isothiocyanate; forward scatter; global accuracy; head and neck squamous cell carcinoma; human immunodeficiency virus; human leukocyte antigen; likelihood ratio; mAbs; monoclonal antibodies; negative predictive value; phycoerythrin; positive predictive value; receiver operating characteristic; side scatter; tri-color; tumor classification: Tumor, nodes (lymph nodes) metastasis; world health organization