title: Characterization by culture-dependent and culture-independent methods of the bacterial population of suckling-lamb packaged in different atmospheres.
abstract: This study offers insight into the dynamics of bacterial populations in fresh cuts of suckling lamb under four different atmospheric conditions: air (A), and three Modified Atmosphere Packaging (MAP) environments, 15%O2/30%CO2/55%N2 (C, commercial), 70%O2/30%CO2 (O), and 15%O2/85%CO2 (H) for 18 days. Microbial analyses by both conventional methods and PCR-DGGE were performed. Controversial and surprising results emerged from comparing both methods in relation to the genus Pseudomonas. Thus, conventional methods detected the presence of high numbers of Pseudomonas colonies, although PCR-DGGE only detected this genus in air-packaged samples. PCR-DGGE detected higher microbial diversity in the control samples (A) than in the modified atmospheres (C, O, H), having atmosphere H the fewest number of species. Brochothrix thermosphacta, LAB (Carnobacterium divergens and Lactobacillus sakei), and Escherichia spp. were detected in all the atmospheres throughout storage. Moreover, previously undescribed bacteria from lamb meat such as Enterobacter hormaechei, Staphylococcus equorum and Jeotgalicoccus spp. were also isolated in this study by DGGE. Additionally, qPCR analysis was used to detect and characterize strains of Escherichia coli. Virulence genes (stx1, stx2 and eae) were detected throughout storage in 97% of the samples. A high CO2 atmosphere was the most effective packaging combination doubling storage time in comparison with commercial atmosphere.
authors.affiliation: Department of Biotechnology and Food Science, University of Burgos, Burgos, Spain.
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Osés
authors.forename: Ana M
authors.initials: AM
authors.lastname: Diez
authors.forename: Beatriz
authors.initials: B
authors.lastname: Melero
authors.forename: Pieternel A
authors.initials: PA
authors.lastname: Luning
authors.forename: Isabel
authors.initials: I
authors.lastname: Jaime
authors.forename: Jordi
authors.initials: J
authors.lastname: Rovira
mesh_terms: D000818:Animals; D001272:Atmosphere; D001419:Bacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D018857:Food Packaging / Q000379:methods*; D008460:Meat / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012756:Sheep
keywords: MAP; PCR-DGGE; Shelf-life; Suckling lamb; qPCR