title: Image analysis methods for assessment of H2O2 production and Plasmopara viticola development in grapevine leaves: application to the evaluation of resistance to downy mildew.
abstract: The grapevine downy mildew (Plasmopara viticola) provokes severe damages and destroys the harvest in the absence of an effective protection. Numerous fungicide treatments are thus generally necessary. To promote a sustainable production, alternative strategies of protection including new antifungal molecules, resistant genotypes or elicitor-induced resistance are under trial. To evaluate the relevance of these strategies, resistance tests are required. In this context, three image analysis methods were developed to read the results of tests performed to assess P. viticola sporulation and mycelial development, and H(2)O(2) production in leaves. They have been validated using elicitors of plant defenses. These methods are reliable, innovative, rapid, and their modular concept allows their further adaptation to other host-pathogen systems.
authors.affiliation: Université de Bourgogne, UMR AgroSup/INRA/uB 1347 Agroécologie, ERL CNRS 6300, BP 86510, F-21000 Dijon, France. Electronic address: Ian.Li-Kim-Khiook@u-bourgogne.fr.
authors.forename: Ian Li
authors.initials: IL
authors.lastname: Kim Khiook
authors.forename: Charles
authors.initials: C
authors.lastname: Schneider
authors.forename: Marie-Claire
authors.initials: MC
authors.lastname: Heloir
authors.forename: Benjamin
authors.initials: B
authors.lastname: Bois
authors.forename: Xavier
authors.initials: X
authors.lastname: Daire
authors.forename: Marielle
authors.initials: M
authors.lastname: Adrian
authors.forename: Sophie
authors.initials: S
authors.lastname: Trouvelot
mesh_terms: D060467:Disease Resistance; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D009868:Oomycetes / Q000254:growth & development*; D010935:Plant Diseases / Q000469:parasitology*; D018515:Plant Leaves / Q000378:metabolism / Q000469:parasitology*; D015203:Reproducibility of Results; D027843:Vitis / Q000378:metabolism / Q000469:parasitology*
chemical_list: D006861:Hydrogen Peroxide
keywords: Downy mildew; Grapevine; H(2)O(2); Image analysis; Plasmopara viticola; Resistance tests