title: Cryoprotection of fibroblasts by carboxylated poly-L-lysine upon repeated freeze/thaw cycles.
abstract: The cryoprotection of carboxylated h-poly-L-lysine (COOH-PLL) was investigated on fibroblasts [L-929 cells and human dermal fibroblasts (HDFs)] during multiple freeze/thaw cycles. COOH-PLL was not toxic to two fibroblast cell types even at 25% (w/v) concentration, whereas dimethylsulphoxide (DMSO) was highly toxic over 3.13% (v/v). When L-929 cells were subjected to 5 freeze/thaw cycles, the media containing 7.5% (w/v) COOH-PLL maintained cell morphology and significantly suppressed growth inhibition as well as cell detachment (P < 0.05). The result was comparable to the media containing 10% (v/v) DMSO. For HDFs, COOH-PLL could effectively retain cell viability and proliferation against 3 freeze/thaw cycles. Cell viability of HDFs was decreased after 5 freeze/thaw cycles, but COOH-PLL exerted better cryoprotection. The cell type might account for the difference in the observations. The data demonstrated that COOH-PLL is a good cryoprotectant for mammalian cells against repeated freeze/thaw cycles, and may be used for cell preservation in fields of cell transplantation, tissue engineering and regenerative medicine.
authors.affiliation: Department of Applied Nanoscience, Pusan National University, Busan, Korea.
authors.forename: O S
authors.initials: OS
authors.lastname: Jin
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
authors.forename: Y C
authors.initials: YC
authors.lastname: Shin
authors.forename: E J
authors.initials: EJ
authors.lastname: Lee
authors.forename: J J
authors.initials: JJ
authors.lastname: Lee
authors.forename: K
authors.initials: K
authors.lastname: Matsumura
authors.forename: S H
authors.initials: SH
authors.lastname: Hyon
authors.forename: D W
authors.initials: DW
authors.lastname: Han
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000378:metabolism* / Q000633:toxicity; D020405:Dermis / Q000166:cytology; D004121:Dimethyl Sulfoxide / Q000378:metabolism / Q000633:toxicity; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D005615:Freezing; D006801:Humans; D007231:Infant, Newborn; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000633:toxicity; D051379:Mice
chemical_list: D003451:Cryoprotective Agents; D008239:Lysine; D004121:Dimethyl Sulfoxide