title: [Impact of ethane dimethane sulfonate on the histological structures of seminal vesicles in adult rats].
abstract: OBJECTIVE
  To study the effect of ethane dimethane sulfonate (EDS) injection on the volumes of different histological structures in the seminal vesicles of adult rats.
  
  
  METHODS
  Twenty-seven male SD rats aged approximately 90 days were randomly divided into a control group (n = 14) and an EDS group (n = 13) to receive one intraperitoneal injection of normal saline and EDS (75 mg/kg bodyweight), respectively. At 7 and 12 days after treatment, the unilateral seminal vesicles were removed, methacrylate resin-embedded sections prepared and the total volumes of various structures in the seminal vesicles estimated using stereological methods.
  
  
  RESULTS
  EDS treatment almost completely destroyed the Leydig cells in the testis, resulting in a drastic testosterone deficiency. The volume of the seminal vesicle (including the coagulating gland attached to the vesicle) was decreased by 53% in the 7 d EDS group (n = 6) in comparison with the 7 d control group (n = 7) ([138.2 +/- 12.9] vs [64.9 +/- 3.6] mm3, P < 0.01), but showed no significant difference between the 7 d and the 12 d EDS (n = 7) groups ([64.9 +/- 3.6] vs [55.4 +/- 7.7] mm3, P > 0.05). The total volumes of the glandular lumen, glandular epithelium, smooth muscular layer and adventitia were decreased by 96.7, 80.3, 57.6 and 67.0%, respectively, in the 12 d EDS group as compared with the 12 d control group (n = 7).
  
  
  CONCLUSIONS
  EDS induces drastic testosterone deficiency in adult rats, and significantly reduces the total volumes of the seminal vesicle lumen, glandular epithelium, smooth muscular layer and adventitia.
authors.affiliation: Morphometric Research Laboratory, North Sichuan Medical College, Nanchong, Sichuan 637007, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Guo
authors.forename: Zheng-Wei
authors.initials: ZW
authors.lastname: Yang
mesh_terms: D000818:Animals; D007985:Leydig Cells / Q000187:drug effects; D008297:Male; D008698:Mesylates / Q000494:pharmacology*; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012669:Seminal Vesicles / Q000187:drug effects* / Q000473:pathology; D013737:Testis / Q000166:cytology / Q000187:drug effects* / Q000473:pathology
chemical_list: D008698:Mesylates; C002994:ethylene dimethanesulfonate