title: Recombinant bacteriophages as gold binding bio-templates.
abstract: Bacteriophages are nano-sized virion particles infecting bacteria. In this study, it is shown that metal binding properties of filamentous fd-bacteriophages can be enhanced by genetic engineering. Quartz crystal microbalance (QCM) analyses, UV-vis absorption spectra measurements and scanning electron microscopy (SEM) imaging revealed that expression of MMM short amino acid sequence on major coat protein p8 facilitates recombinant MMM-phage binding to Au surfaces and nanoparticles (NPs) via gold-sulfur (AuS) interaction. Electroless deposition of Au particles on phage assemblies was investigated upon chemical reduction reaction with NaBH4 at different HAuCl4 precursor concentrations. Energy dispersive X-ray spectroscopy (EDX) measurements confirmed the presence of Au on both AuNP decorated and chemically metallized phage structures. Further studies on patterning and controlled immobilization of recombinant bacteriophages on specific surfaces may contribute to bio-templated nanowire development field and biosensor application studies.
authors.affiliation: Convergence Bioscience Group, Nanomedicine Team, Korea Institute of Science and Technology, Europe Forschungsgesellschaft mbH, Campus E 71, D-66123 Saarbrücken, Germany. Electronic address: n.korkmaz@kist-europe.de.
authors.forename: Nuriye
authors.initials: N
authors.lastname: Korkmaz
mesh_terms: D017104:Bacteriophage M13 / Q000235:genetics* / Q000378:metabolism* / Q000648:ultrastructure; D001665:Binding Sites; D015374:Biosensing Techniques; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D005818:Genetic Engineering; D006046:Gold; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D053770:Nanowires; D058525:Quartz Crystal Microbalance Techniques; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013052:Spectrometry, X-Ray Emission; D013053:Spectrophotometry; D013499:Surface Properties
chemical_list: D036022:Capsid Proteins; D011994:Recombinant Proteins; D006046:Gold
keywords: Bacteriophage; Genetic engineering; Gold; Metallization; Nanoparticle; SEM