title: Identification of serum biomarkers for occupational medicamentosa-like dermatitis induced by trichloroethylene using mass spectrometry.
abstract: Occupational medicamentosa-like dermatitis induced by trichloroethylene (OMLDT) is an autoimmune disease and it has become a serious occupational health hazard. In the present study, we collected fasting blood samples from patients with OMLDT (n=18) and healthy volunteers (n=33) to explore serum peptidome patterns. Peptides in sera were purified using weak cation exchange magnetic beads (MB-WCX), and analyzed by matrix-assisted laser desorption ionization time-of-flight-mass spectrometry (MALDI-TOF-MS) and ClinProTools bioinformatics software. The intensities of thirty protein/peptide peaks were significantly different between the healthy control and OMLDT patients. A pattern of three peaks (m/z 2106.3, 2134.5, and 3263.67) was selected for supervised neural network (SNN) model building to separate the OMLDT patients from the healthy controls with a sensitivity of 95.5% and a specificity of 73.8%. Furthermore, two peptide peaks of m/z 4091.61 and 4281.69 were identified as fragments of ATP-binding cassette transporter family A member 12 (ABCA12), and cationic trypsinogen (PRRS1), respectively. Our findings not only show that specific proteomic fingerprints in the sera of OMLDT patients can be served as a differentiated tool of OMLDT patients with high sensitivity and high specificity, but also reveal the novel correlation between OMLDT with ABC transports and PRRS1, which will be of potential value for clinical and mechanistic studies of OMLDT.
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Guangdong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
authors.forename: Wen-Xu
authors.initials: WX
authors.lastname: Hong
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Peiwu
authors.initials: P
authors.lastname: Huang
authors.forename: Xifei
authors.initials: X
authors.lastname: Yang
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Ren
authors.forename: Jinbo
authors.initials: J
authors.lastname: Ye
authors.forename: Haiyan
authors.initials: H
authors.lastname: Huang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Tang
authors.forename: Guifeng
authors.initials: G
authors.lastname: Zhou
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Huang
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Zhuang
authors.forename: Jianjun
authors.initials: J
authors.lastname: Liu
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000097:blood; D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D019295:Computational Biology; D003875:Drug Eruptions / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D010455:Peptides / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D012680:Sensitivity and Specificity; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014241:Trichloroethylene / Q000633:toxicity*; D014357:Trypsin / Q000097:blood; D055815:Young Adult
chemical_list: C473801:ABCA12 protein, human; D018528:ATP-Binding Cassette Transporters; D015415:Biomarkers; D010455:Peptides; D014241:Trichloroethylene; C489303:PRSS1 protein, human; D014357:Trypsin
keywords: Biomarkers; ClinProTools; MALDI-TOF-MS; Occupational medicamentosa-like dermatitis induced by trichloroethylene (OMLDT)