title: Global study of IS6110 in a successful Mycobacterium tuberculosis strain: clues for deciphering its behavior and for its rapid detection.
abstract: The Mycobacterium tuberculosis insertion sequence IS6110, besides being a very useful tool in molecular epidemiology, seems to have an impact on the biology of bacilli. In the present work, we mapped the 12 points of insertion of IS6110 in the genome of a successful strain named M. tuberculosis Zaragoza (which has been referred to as the MTZ strain). This strain, belonging to principal genetic group 3, caused a large unsuspected tuberculosis outbreak involving 85 patients in Zaragoza, Spain, in 2001 to 2004. The mapping of the points of insertion of IS6110 in the genome of the Zaragoza strain offers clues for a better understanding of the adaptability and virulence of M. tuberculosis. Surprisingly, the presence of one copy of IS6110 was found in Rv2286c, as was recently described for a successful Beijing sublineage. As a result of this analysis, a rapid method for detecting this particular M. tuberculosis strain has been designed.
authors.affiliation: IIS Aragón, Hospital Universitario Miguel Servet, Zaragoza, Spain.
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: Millán-Lou
authors.forename: Ana Isabel
authors.initials: AI
authors.lastname: López-Calleja
authors.forename: Cristina
authors.initials: C
authors.lastname: Colmenarejo
authors.forename: Maria Antonia
authors.initials: MA
authors.lastname: Lezcano
authors.forename: Maria Asunción
authors.initials: MA
authors.lastname: Vitoria
authors.forename: Patricia
authors.initials: P
authors.lastname: del Portillo
authors.forename: Isabel
authors.initials: I
authors.lastname: Otal
authors.forename: Carlos
authors.initials: C
authors.lastname: Martín
authors.forename: Sofía
authors.initials: S
authors.lastname: Samper
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004196:Disease Outbreaks; D016680:Genome, Bacterial; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D013030:Spain / Q000453:epidemiology; D014376:Tuberculosis / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology*
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial