title: Analysis of the coverage capacity of the StreptInCor candidate vaccine against Streptococcus pyogenes.
abstract: Streptococcus pyogenes is responsible for infections as pharyngitis, sepsis, necrotizing fasciitis and streptococcal toxic shock syndrome. The M protein is the major bacterial antigen and consists of both polymorphic N-terminal portion and a conserved region. In the present study, we analyzed the in vitro ability of StreptInCor a C-terminal candidate vaccine against S. pyogenes to induce antibodies to neutralize/opsonize the most common S. pyogenes strains in Sao Paulo by examining the recognition by sera from StreptInCor immunized mice. We also evaluated the presence of cross-reactive antibodies against human heart valve tissue. Anti-StreptInCor antibodies were able to neutralize/opsonize at least 5 strains, showing that immunization with StreptInCor is effective against several S. pyogenes strains and can prevent infection and subsequent sequelae without causing autoimmune reactions.
authors.affiliation: Heart Institute (InCor), School of Medicine, University of Sao Paulo, Sao Paulo, Brazil; Immunology Investigation Institute, National Institute for Science and Technology, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Karine M
authors.initials: KM
authors.lastname: De Amicis
authors.affiliation: Heart Institute (InCor), School of Medicine, University of Sao Paulo, Sao Paulo, Brazil; Immunology Investigation Institute, National Institute for Science and Technology, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Samar
authors.initials: S
authors.lastname: Freschi de Barros
authors.affiliation: Heart Institute (InCor), School of Medicine, University of Sao Paulo, Sao Paulo, Brazil; Immunology Investigation Institute, National Institute for Science and Technology, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Raquel E
authors.initials: RE
authors.lastname: Alencar
authors.affiliation: Heart Institute (InCor), School of Medicine, University of Sao Paulo, Sao Paulo, Brazil; Immunology Investigation Institute, National Institute for Science and Technology, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Edilberto
authors.initials: E
authors.lastname: Postól
authors.affiliation: Heart Institute (InCor), School of Medicine, University of Sao Paulo, Sao Paulo, Brazil; Immunology Investigation Institute, National Institute for Science and Technology, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Carlo de Oliveira
authors.initials: Cde O
authors.lastname: Martins
authors.affiliation: Heart Institute (InCor), School of Medicine, University of Sao Paulo, Sao Paulo, Brazil; Immunology Investigation Institute, National Institute for Science and Technology, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Helen Andrade
authors.initials: HA
authors.lastname: Arcuri
authors.affiliation: Biotechnology Center, Butantan Institute, Sao Paulo, Brazil.
authors.forename: Cibelly
authors.initials: C
authors.lastname: Goulart
authors.affiliation: Heart Institute (InCor), School of Medicine, University of Sao Paulo, Sao Paulo, Brazil; Immunology Investigation Institute, National Institute for Science and Technology, University of Sao Paulo, Sao Paulo, Brazil; Clinical Immunology and Allergy Division, School of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Jorge
authors.initials: J
authors.lastname: Kalil
authors.affiliation: Heart Institute (InCor), School of Medicine, University of Sao Paulo, Sao Paulo, Brazil; Immunology Investigation Institute, National Institute for Science and Technology, University of Sao Paulo, Sao Paulo, Brazil. Electronic address: luizagui@usp.br.
authors.forename: Luiza
authors.initials: L
authors.lastname: Guilherme
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D057134:Antibodies, Neutralizing / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D015551:Autoimmunity; D003429:Cross Reactions; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008943:Mitral Valve / Q000276:immunology; D010587:Phagocytosis; D013290:Streptococcal Infections / Q000517:prevention & control*; D022541:Streptococcal Vaccines / Q000276:immunology*; D013297:Streptococcus pyogenes
chemical_list: D000907:Antibodies, Bacterial; D057134:Antibodies, Neutralizing; D000942:Antigens, Bacterial; D022541:Streptococcal Vaccines
keywords: Immune response; Immunization coverage; Rheumatic fever; Streptococcus pyogenes; Vaccine