title: [Effects of ABA on tanshinones accumulation of Salvia miltiorrhiza hairy root].
abstract: OBJECTIVE
  To study the effects of Abscisic Acid (ABA) on the accumulation of tanshinones in suspension cultures of Salvia miltiorrhiza hairy roots.
  
  
  METHODS
  After suspension culture of Salvia miltiorrhiza hairy roots induced by agrobacterium rhizogenes ATCC15834 for 20 days, the ABA was added into suspension cultures, the contents of tanshinones (including tanshinone I , cryptotanshinone, dihydrotanshinon I and tanshinone II A) were detected by HPLC after 7 days.
  
  
  RESULTS
  The growth of Salvia miltiorrhiza hairy roots was inhibited by ABA. 20 micromol/L ABA treatment had no significant effect. The dry weight of Salvia miltiorrhiza hairy roots of other ABA treatments reduced to 65.2% ,71% and 56.5% of the control, respectively. Different levels of ABA could significantly increased the yields of four tanshinones in Salvia miltiorrhiza hairy roots. The yields of tanshinone I , cryptotanshinone, dihydrotanshinon I and tanshinone II A were significantly increased to 5.4, 9. 5, 1.5 and 1.8 times that of the control group after dealing with 210 micromol/ L ABA.
  
  
  CONCLUSIONS
  ABA can stimulate the accumulation of tanshinones in Salvia miltiorrhiza hairy roots.
authors.affiliation: Department of Life Science, Zhoukou Normal College, Zhoukou 466001, China. shengdongfeng@126.com
authors.forename: Dong-Feng
authors.initials: DF
authors.lastname: Sheng
authors.forename: Yong-Liang
authors.initials: YL
authors.lastname: Zhang
mesh_terms: D045784:Abietanes / Q000378:metabolism*; D000040:Abscisic Acid / Q000494:pharmacology*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism*; D012231:Rhizobium; D027561:Salvia miltiorrhiza / Q000254:growth & development / Q000378:metabolism*; D046509:Tissue Culture Techniques
chemical_list: D045784:Abietanes; C021751:tanshinone; D000040:Abscisic Acid