title: [Study on serum fingerprint of menoprogen].
abstract: OBJECTIVE
  To establish chromatographic fingerprint of components of Menoprogen absorbed into blood by RP-HPLC.
  
  
  METHODS
  Kromasil C18 column was used, with of 0.1% phosphoric acid and acetonitrile as mobile phase in a gradient elution. The flow rate was 1.0 mL/min, the column temperature was 30 degrees C, the detection wavelength was 205 nm. 10 SD rats were administered 10 different batches of Menoprogen respectively.
  
  
  RESULTS
  The fingerprint of Menoprogen was established. 18 common peaks were identified, the similarities were over 0. 95. By comparison drug and blank serum wiith contained drug serum, identified eight prototype components aborbed into blood directly and 10 metabolic components.
  
  
  CONCLUSIONS
  The serum fingerprint of Menoprogen is established for the first time. 18 components are identifided in blood,one of them is hyperoside. It can reveal the change of chemical constituents after ingestion, and provide some data on material basis study in vivo for Menoprogen.
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China. ruwenwen999@126.com
authors.forename: Wen-Wen
authors.initials: WW
authors.lastname: Ru
authors.forename: Guang-Lin
authors.initials: GL
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Wei
authors.forename: Ye
authors.initials: Y
authors.lastname: Lv
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002214:Capsules; D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics*; D010946:Plants, Medicinal / Q000737:chemistry; D011794:Quercetin / Q000031:analogs & derivatives* / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C529990:menoprogen; C021304:hyperoside; D011794:Quercetin