title: Protection of renal function by green tea extract during Plasmodium berghei infection.
abstract: Impairment of renal function from oxidative stress during malaria infection is one of the leading causes of death in endemic areas. Since blood urea nitrogen and creatinine levels in plasma can be used as markers for monitoring renal damage, this study investigated the effect of green tea extract on reduction of blood urea nitrogen and creatinine levels during malaria infection using Plasmodium berghei ANKA infected mice as in vivo model. For in vivo testing, ICR mice were infected with 1 × 10(7) parasitized erythrocytes and green tea extract was subsequently administered orally twice a day for 10 consecutive days. Parasitemia was estimated by standard microscopy, and blood urea nitrogen and creatinine levels in plasma were also measured. It was found that parasitemia kept increasing until animal death, and is strongly correlated with high blood urea nitrogen and creatinine. The highest levels of blood urea nitrogen and creatinine in plasma were found on day 10 after infection. However, blood urea nitrogen and creatinine levels in plasma were reduced and decreased significantly (p<0.01) in green tea extract treated mice, compared with untreated group. It can be concluded that green tea extract can protect and maintain renal function during malaria infection, and this extract can be developed for use as a supplement and combination therapy.
authors.affiliation: Department of Clinical Chemistry, Faculty of Medical Technology, Western University, Kanchanaburi 71170, Thailand. Electronic address: voravuthsomsak@gmail.com.
authors.forename: Voravuth
authors.initials: V
authors.lastname: Somsak
authors.forename: Ubonwan
authors.initials: U
authors.lastname: Jaihan
authors.forename: Somdet
authors.initials: S
authors.lastname: Srichairatanakool
authors.forename: Chairat
authors.initials: C
authors.lastname: Uthaipibull
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001806:Blood Urea Nitrogen; D028241:Camellia sinensis / Q000737:chemistry*; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D007668:Kidney / Q000378:metabolism; D008288:Malaria / Q000188:drug therapy*; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010962:Plasmodium berghei / Q000187:drug effects*; D013047:Specific Pathogen-Free Organisms; D016896:Treatment Outcome
chemical_list: D000962:Antimalarials; D010936:Plant Extracts; D003404:Creatinine
keywords: Green tea extract; Plasmodium berghei; Renal function