title: Chitinophaga polysaccharea sp. nov., an exopolysaccharide-producing bacterium isolated from the rhizoplane of Dioscorea japonica.
abstract: A novel strain designated MRP-15(T), belonging to the class Sphingobacteriia (phylum Bacteroidetes), was isolated from the rhizoplane of Dioscorea japonica in South Korea and was characterized taxonomically using a polyphasic approach. The strain was found to comprise Gram-stain-negative, aerobic, non-motile, non-spore-forming rods. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain belonged to the genus Chitinophaga but was clearly separated from established species of the genus Chitinophaga. 16S rRNA gene sequence similarities between strain MRP-15(T) and type strains of established species of the genus Chitinophaga ranged from 90.3 to 97.8 %. Phenotypic and chemotaxonomic data (major menaquinone, MK-7; major fatty acids, iso-C15 : 0 and C16 : 1ω5c) supported the affiliation of strain MRP-15(T) with the genus Chitinophaga. Therefore strain MRP-15(T) represents a novel species of the genus Chitinophaga, for which the name Chitinophaga polysaccharea sp. nov. is proposed. The type strain is MRP-15(T) ( = KACC 17184(T) = NCAIMB 02530(T)).
authors.affiliation: Department of Microbial & Nano Materials, Mokwon University, Mokwon Gil-21, Doan-dong, Seo-gu, Daejeon, Republic of Korea.
authors.forename: Song-Ih
authors.initials: SI
authors.lastname: Han
authors.affiliation: Department of Microbial & Nano Materials, Mokwon University, Mokwon Gil-21, Doan-dong, Seo-gu, Daejeon, Republic of Korea.
authors.forename: Hyo-Jin
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Institute of Microbial Ecology and Resources, Mokwon University, Mokwon Gil-21, Doan-dong, Seo-gu, Daejeon, Republic of Korea.|Department of Microbial & Nano Materials, Mokwon University, Mokwon Gil-21, Doan-dong, Seo-gu, Daejeon, Republic of Korea.
authors.forename: Kyung-Sook
authors.initials: KS
authors.lastname: Whang
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D027742:Dioscorea / Q000382:microbiology*; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7