title: [The immune-enzyme technique of detection of Ca2+ ATPase of sarcoplasmic reticulum--a new biologic marker of acute cardiac infarction].
abstract: The immune-enzyme system of testing was developed to detect the level of Ca2+ ATPase of sarcoplasmic reticulum using monoclonal IgM and IgG antibodies to Ca2+ ATPase. The clinical approbation of the technique was carried out using the sample of 19 patients with acute cardiac infarction Q validated by the increase of content of such common markers of myocardium necrosis as troponin T and creatine phosphokinase MB. The increase of the level of Ca2+ ATPase of sarcoplasmic reticulum was detected in all patients. This occurrence is detected approximately in 4-6 hours after debut and it disappears in 144 hours (6 days). At the same time, Ca2+ ATPase was not detected in 10 Patients with acute traumatic damages of skeleton musculature, in 10 patients with chronic renal failure under hemodialysis and in 20 patients with acute coronary syndrome without rise of ST-segment of cardiogram. These facts testify rather high specificity and sensitivity of the developed technique of detection of the level of Ca2+ ATPase of sarcoplasmic reticulum in blood as biological marker of myocardium necrosis.
authors.forename: L M
authors.initials: LM
authors.lastname: Tchobanu
authors.forename: S I
authors.initials: SI
authors.lastname: Syrbu
authors.forename: I M
authors.initials: IM
authors.lastname: Popovitch
authors.forename: V M
authors.initials: VM
authors.lastname: Ivanova
authors.forename: V S
authors.initials: VS
authors.lastname: Gudumak
authors.forename: M I
authors.initials: MI
authors.lastname: Popovitch
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D052279:Creatine Kinase, MB Form / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009203:Myocardial Infarction / Q000175:diagnosis* / Q000473:pathology; D009336:Necrosis; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000097:blood*; D014336:Troponin / Q000097:blood
chemical_list: D015415:Biomarkers; D014336:Troponin; D052279:Creatine Kinase, MB Form; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases