title: Porcine alpha interferon inhibit Japanese encephalitis virus replication by different ISGs in vitro.
abstract: Japanese encephalitis virus (JEV) causes serious zoonosis in South Asia, Southeast Asia and other areas. Pigs are an important reservoir for this virus in nature. The treatment of JEV infection in pigs is important for controlling the prevalence of JEV in humans and economic losses in pig farming. In this study, we selected a high activity porcine alpha-interferon to inhibit JEV replication in porcine kidney cell lines (PK-15). Alpha interferon exhibited high antiviral activity against JEV; the expression of three interferon-stimulated genes (ISGs), ISG15, Mx2 and OAS L, increased significantly after interferon treatment. Furthermore, we verified the anti-JEV effect of these ISGs by RNAi and overexpression. Mx2 and OAS L exhibited strong anti-JEV effects in PK-15 cells. Based on these novel results, alpha interferon (IFN-α) should be considered to be a potential drug against JEV in pigs.
authors.affiliation: Key Laboratory of Animal Disease Diagnosis and Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China; Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No. 518, Ziyue Road, Shanghai 200241, PR China. Electronic address: keliu1982@aliyun.com.
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
authors.forename: Xuewen
authors.initials: X
authors.lastname: Liao
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Huijuan
authors.initials: H
authors.lastname: Yao
authors.forename: Shengchao
authors.initials: S
authors.lastname: Fan
authors.forename: Puyan
authors.initials: P
authors.lastname: Chen
authors.forename: Denian
authors.initials: D
authors.lastname: Miao
mesh_terms: D000818:Animals; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004664:Encephalitis Virus, Japanese / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D050835:Interferon Regulatory Factors / Q000378:metabolism* / Q000502:physiology; D016898:Interferon-alpha / Q000494:pharmacology*; D007668:Kidney / Q000166:cytology / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D050835:Interferon Regulatory Factors; D016898:Interferon-alpha
keywords: IFN-stimulated genes; Japanese encephalitis virus; Porcine alpha interferon