title: Rhesus-D zygosity and hemolytic disease of fetus and newborn.
abstract: Alloimmunization against the Rhesus-D (RhD) antigen still remains as a major cause of hemolytic disease of fetus and newborn (HDFN). Determination of paternal RhDzygosity is performed by molecular testing and is valuable for the management of alloimmunized pregnant women. A 30-year-old pregnant woman with AB negative blood group presented with two consecutive abortions and no history of blood transfusion. By application of the antibody screening, identification panel, and selected cells, she was found to be highly alloimmunized. RhDzygosity was performed on her partner and was shown to be homozygous for RhD. The sequence- specific priming-polymerase chain reaction used in this report is essential to establish whether the mother requires an appropriate immunoprophylaxis or the fetus is at risk of HDFN.
authors.affiliation: Department of Immunohematology, Central Lab. of Iranian Blood Transfusion Organization (IBTO), Tehran, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Moghaddam
authors.forename: Amirali
authors.initials: A
authors.lastname: Naghi
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Hassani
authors.forename: Sedighe
authors.initials: S
authors.lastname: Amini
keywords: Rhesus-Dzygosity; anti-D; hemolytic disease of fetus and newborn