title: Comparative quantitative analysis of headspace volatiles and their association with BADH2 marker in non-basmati scented, basmati and non-scented rice (Oryza sativa L.) cultivars of India.
abstract: For the first time, 91 Indian rice (Oryza sativa L.) cultivars, belonging to non-basmati scented (77), basmati (9) and non-scented (5) categories, were quantitatively analysed for 23 headspace volatiles. In addition, the BADH2 marker related to 2-acetyl-1-pyrroline (2AP) synthesis is validated among the cultivars representing these categories. The non-basmati scented cultivars (15) excelled in 2-acetyl-1-pyrroline (2AP) content compared to basmati cultivars. The major aroma volatile (2AP) correlated positively with 1-tetradecene and indole, and negatively with benzyl alcohol. On the basis of nonanal, octanal, decanal and 1-octen-3-ol contents, basmati cultivars were different from non-scented cultivars. Benzyl alcohol, 2-phenylethanol, 2-amino acetophenone, indole, 1-hexanol and nonanoic acid exhibited significant variation among rice categories under study. This study reports 16 non-basmati scented cultivars with variations in the BADH2 locus rendering a marker based on 8bp deletion in BADH2 as unsuitable for MAS in rice cultivars under study.
authors.affiliation: Department of Botany, University of Pune, Pune 411007, India; Molecular Biology Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
authors.forename: Sarika V
authors.initials: SV
authors.lastname: Mathure
authors.forename: Narendra
authors.initials: N
authors.lastname: Jawali
authors.forename: Ratnakar J
authors.initials: RJ
authors.lastname: Thengane
authors.forename: Altafhusian B
authors.initials: AB
authors.lastname: Nadaf
mesh_terms: D050728:Betaine-Aldehyde Dehydrogenase / Q000235:genetics*; D005819:Genetic Markers; D007194:India; D009812:Odorants / Q000032:analysis; D012275:Oryza / Q000737:chemistry* / Q000145:classification / Q000201:enzymology / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000378:metabolism
chemical_list: D005819:Genetic Markers; D010940:Plant Proteins; D055549:Volatile Organic Compounds; D050728:Betaine-Aldehyde Dehydrogenase
keywords: BADH2 marker; Headspace volatiles; Indian non-basmati scented rice; SPME; Solid phase micro extraction