title: Peroxiredoxin 1 is a tumor-associated antigen in esophageal squamous cell carcinoma.
abstract: Peroxiredoxin 1 (Prdx1) is an antioxidant and plays an important role in H2O2-mediated cell signaling. We previously found that the expression level of Prdx1 was elevated in esophagus squamous cell carcinoma (ESCC) tissue using a proteomics approach. Since overexpressed protein can induce an autoimmune response, to further examine whether serum from ESCC patients exhibits immunoreactivity against Prdx1, autoantibody responses to Prdx1 were evaluated by ELISA, western blotting and indirect immunofluorescence assay in sera from patients with ESCC and normal individuals. Immunohistochemical study with tissue array slides and western blot analysis with cancer cell lines were also performed to analyze the protein expression profiles of Prdx1 in ESCC tissues and cancer cell lines. The results demonstrated that the positive rate of autoantibody against Prdx1 in ESCC sera was 13.2% (9/68), whereas this rate was 0% (0/89) in normal individuals. Data also showed that expression of Prdx1 was significantly increased in ESCC tissues when compared to expression in paired adjacent normal tissues (P<0.05). The data indicate that Prdx1 may contribute to malignant transformation of the esophagus, and may be used as a biomarker in the immunodiagnosis of ESCC.
authors.affiliation: Henan Key Laboratory of Tumor Epidemiology and College of Public Health, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Ren
authors.forename: Hua
authors.initials: H
authors.lastname: Ye
authors.forename: Liping
authors.initials: L
authors.lastname: Dai
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.forename: Xinxin
authors.initials: X
authors.lastname: Liu
authors.forename: Yurong
authors.initials: Y
authors.lastname: Chai
authors.forename: Qing
authors.initials: Q
authors.lastname: Shao
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Ningjing
authors.initials: N
authors.lastname: Lei
authors.forename: Bo
authors.initials: B
authors.lastname: Peng
authors.forename: Wu
authors.initials: W
authors.lastname: Yao
authors.forename: Jianying
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics* / Q000276:immunology; D001323:Autoantibodies; D014408:Biomarkers, Tumor / Q000097:blood / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000235:genetics* / Q000276:immunology* / Q000473:pathology; D045744:Cell Line, Tumor; D004938:Esophageal Neoplasms / Q000097:blood / Q000235:genetics* / Q000276:immunology* / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D015972:Gene Expression Regulation, Neoplastic / Q000276:immunology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D054464:Peroxiredoxins / Q000097:blood / Q000235:genetics* / Q000276:immunology; D015398:Signal Transduction
chemical_list: D000951:Antigens, Neoplasm; D001323:Autoantibodies; D014408:Biomarkers, Tumor; D006861:Hydrogen Peroxide; C515940:PRDX1 protein, human; D054464:Peroxiredoxins