title: [Expression of carbonic anhydrase II in cultured rat osteoclasts after stress stimulation].
abstract: OBJECTIVE
  To test expression of carbonic anhydrase II (Ca II) mRNA in osteoclasts which were applied with fluid shear stress.
  
  
  METHODS
  The bone marrow cells of Sprague-Dawley rats were cultured with the presence of 1,25-(OH)2D3 and dexamethasone. The osteoclast-like cells were identified by tartrate-resistant acid phosphatase(TRAP) staining and scanning electron microscope (SEM) observation, then purified with trypsin/ethylenediamine tetraacetic acid (EDTA). Different values and lasting time of steady fluid shear stress were exerted on the osteoclasts with parallel plate flow system. The Ca II expression of osteoclasts were detected by real time reverse transcription-polymerase chain reaction(RT-PCR) and nested polymerase chain reaction(PCR).
  
  
  RESULTS
  The levels of Ca II mRNA were down-regulated correspondingly with the increase of stress and time (P < 0.05).
  
  
  CONCLUSIONS
  It's indicated that steady fluid shear stress within a certain range may down-regulate the expression of Ca II in osteoclasts.
authors.affiliation: Dept. of Stomatology, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Dong
authors.forename: Qian
authors.initials: Q
authors.lastname: Xia
authors.forename: Jianjiang
authors.initials: J
authors.lastname: Zhou
authors.forename: Hong
authors.initials: H
authors.lastname: Ma
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Xing
authors.initials: X
authors.lastname: Liang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D024402:Carbonic Anhydrase II; D002478:Cells, Cultured; D010010:Osteoclasts; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical
chemical_list: D012333:RNA, Messenger; D024402:Carbonic Anhydrase II