title: Genetic diversity of Oenoccoccus oeni isolated from wines treated with phenolic extracts as antimicrobial agents.
abstract: Molecular techniques have been applied to study the evolution of wine-associated lactic acid bacteria from red wines produced in the absence and presence of antimicrobial phenolic extracts, eucalyptus leaves and almond skins, and to genetically characterize representative Oenococcus oeni strains. Monitoring microbial populations by PCR-DGGE targeting the rpoB gene revealed that O. oeni was, as expected, the species responsible for malolactic fermentation (MLF). Representative strains from both extract-treated and not-treated wines were isolated and all were identified as O. oeni species, by 16S rRNA sequencing. Typing of isolated O. oeni strains based on the mutation of the rpoB gene suggested a more favorable adaptation of L strains (n = 63) than H strains (n = 3) to MLF. Moreover, PFGE analysis of the isolated O. oeni strains revealed 27 different genetic profiles, which indicates a rich biodiversity of indigenous O. oeni species in the winery. Finally, a higher number of genetic markers were shown in the genome of strains from control wines than strains from wines elaborated with phenolic extracts. These results provide a basis for further investigation of the molecular and evolutionary mechanisms leading to the prevalence of O. oeni in wines treated with polyphenols as inhibitor compounds.
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación (CIAL), CSIC-UAM, CEI UAM+CSIC, Campus de Cantoblanco, Universidad Autónoma de Madrid, 28049 Madrid, Spain.
authors.forename: Almudena
authors.initials: A
authors.lastname: García-Ruiz
authors.forename: Raquel
authors.initials: R
authors.lastname: Tabasco
authors.forename: Teresa
authors.initials: T
authors.lastname: Requena
authors.forename: Olivier
authors.initials: O
authors.lastname: Claisse
authors.forename: Aline
authors.initials: A
authors.lastname: Lonvaud-Funel
authors.forename: Begoña
authors.initials: B
authors.lastname: Bartolomé
authors.forename: M Victoria
authors.initials: MV
authors.lastname: Moreno-Arribas
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D005052:Eucalyptus / Q000737:chemistry*; D014644:Genetic Variation / Q000187:drug effects; D056585:Oenococcus / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D027861:Prunus / Q000737:chemistry*; D014920:Wine / Q000032:analysis / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D010636:Phenols; D010936:Plant Extracts
keywords: Antimicrobial phenolic extracts; Genetic characterization; Malolactic fermentation; Oenococcus oeni