title: Methods for determining Myc-induced apoptosis.
abstract: Although many oncoproteins promote cell growth and proliferation, some also possess the potential to induce cell death by apoptosis. Deregulated expression of the myc oncogene promotes apoptosis in both cultured cells and in some tissues in vivo. Here we describe techniques to detect Myc-induced apoptosis in vitro using flow cytometry and microscopy and in vivo using immunohistochemical staining.
authors.affiliation: Department of Biochemistry, University of Cambridge, Cambridge, UK.
authors.forename: Dan
authors.initials: D
authors.lastname: Lu
authors.forename: Trevor D
authors.initials: TD
authors.lastname: Littlewood
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008853:Microscopy / Q000379:methods; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics* / Q000378:metabolism
chemical_list: D016271:Proto-Oncogene Proteins c-myc