title: [Analysis of capsaicin in rat plasma using liquid chromatography-tandem mass spectrometry].
abstract: OBJECTIVE
  To establish a simple high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS) system for determination of capsaicin in rat plasma.
  
  
  METHODS
  Rat plasma (100 µl) was extracted with mixed solvent (ethyl acetate:acetone 85:15) after addition of 50 ng/ml internal standard, and analyzed by Agilent 6460 Triple Quadrupole LC/MS system. The detection was performed with multiple reactions monitoring using electrospray ionization. The precursor/product ion transitions were monitored at m/z 306→137 (+Ion mode) for capsaicin. Verapamil was used as the internal standard at m/z455→165.
  
  
  RESULTS
  The analysis time was only 1.5 min in the positive mode; the calibration curve was linear in the concentration range of 1.85-370 ng/ml. The lowest limit of quantification reached 1.85 ng/ml. The extraction recoveries were 77.34%, 70.64% and 78.02% for the three quality control concentration levels (3.7, 37, and 370 ng/ml), respectively.
  
  
  CONCLUSIONS
  The LC-MS/MS system is simple, accurate, reliable and time-saving for determination of trace amount of capsaicin in plasma.
authors.affiliation: Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. E-mail: danieldl@126.com.
authors.forename: Lian
authors.initials: L
authors.lastname: Duan
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yan
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Sijia
authors.initials: S
authors.lastname: Liu
authors.forename: Guofeng
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D002211:Capsaicin / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D010949:Plasma / Q000737:chemistry; D051381:Rats; D053719:Tandem Mass Spectrometry
chemical_list: D002211:Capsaicin