title: [Formation of microbial biofilms in causative agents of acute and chronic pyelonephritis].
abstract: OBJECTIVE
  Study the intensity of formation of microbial biofilms by Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus strains isolated during various forms of pyelonephritis.
  
  
  METHODS
  150 clinical isolates of microorganisms isolated from urine ofpatientswith acute and chronic pyelonephritiswere included into the study. Determination of intensity of film-formation was carried out by staining of the formed biofilms by crystal violet with consequent extraction of the dye and measurement of its concentration in washout solution.
  
  
  RESULTS
  Among causative agents ofpyelonephritis P. aeruginosa isolates had the maximum film-forming ability. The intensity of biofilm formation of these isolates was 2-3 time higher than staphylococcus and enterobacteria strains. Strains isolated from patients with chronic pyelonephritis by ability to form biofilms significantly surpassed strains isolated from acute pyelonephritis patients. A higher ability to form microbial biofilms for microorganisms--causative agents of pyelonephritis progressing against the background ofurolithiasis was noted.
  
  
  CONCLUSIONS
  The ability to form biofilms is determined by both causative agent species and character of the infectious process in which this microorganism participates. Intensive formation of biofilms by E. coli, P. aeruginosa, K. pneumoniae, S. aureus clinical isolates may be an important factor of chronization of urinary tract infections.
authors.forename: L V
authors.initials: LV
authors.lastname: Lagun
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Atanasova
authors.forename: D V
authors.initials: DV
authors.lastname: Tapal'skiĭ
mesh_terms: D000208:Acute Disease; D018441:Biofilms / Q000254:growth & development*; D002908:Chronic Disease; D004926:Escherichia coli / Q000502:physiology*; D005840:Gentian Violet; D006801:Humans; D007711:Klebsiella pneumoniae / Q000502:physiology*; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D011704:Pyelonephritis / Q000150:complications / Q000382:microbiology* / Q000473:pathology / Q000652:urine; D013211:Staphylococcus aureus / Q000502:physiology*; D052878:Urolithiasis / Q000150:complications / Q000382:microbiology* / Q000473:pathology / Q000652:urine
chemical_list: D005840:Gentian Violet