title: Intestinal expression of peptide transporter 1 (PEPT1) at different life stages of Japanese eel, Anguilla japonica.
abstract: The expression of peptide transporter 1 (PEPT1) was investigated at the different life stages of Japanese eel, Anguilla japonica. The cDNA encoding Japanese eel PEPT1 was cloned and sequenced. The hydrophilicity plot analysis of its deduced amino acid sequence showed high similarities with topological features of known PEPT1 molecules in other species. Tissue distribution analysis confirmed that PEPT1 mRNA was detected specifically in the anterior and posterior intestines of adult eel. In eel larvae at 13days post hatching (dph), PEPT1 mRNA expression was mainly detected in the intestinal tract regions. The trypsinogen mRNA was only detected in the gastric region including the pancreas. Intense immunoreaction for PEPT1 was observed in the apical membrane of the intestinal epithelial cells of both larval and adult eel. These results indicated that PEPT1 was an intestine-specific transporter, which was localized at the luminal side of the epithelial cells, suggesting that di/tri-peptide absorption via PEPT1 takes place in the eel intestine. According to the ontogenetic analyses by quantitative PCR, PEPT1 and trypsinogen mRNA expressions were simultaneously increased at 5-7 dph. It is thus assumed that nutrient absorption systems in the intestinal tracts of larvae become functional at this age.
authors.affiliation: Atmosphere and Ocean Research Institute, The University of Tokyo, Kashiwa, Chiba 277-8564, Japan.
authors.forename: Hyojin
authors.initials: H
authors.lastname: Ahn
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Yamada
authors.forename: Akihiro
authors.initials: A
authors.lastname: Okamura
authors.forename: Katsumi
authors.initials: K
authors.lastname: Tsukamoto
authors.forename: Toyoji
authors.initials: T
authors.lastname: Kaneko
authors.forename: Soichi
authors.initials: S
authors.lastname: Watanabe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary; D004524:Eels / Q000235:genetics* / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D007413:Intestinal Mucosa / Q000378:metabolism*; D008675:Metamorphosis, Biological; D000074011:Peptide Transporter 1; D012333:RNA, Messenger / Q000235:genetics*; D027981:Symporters / Q000096:biosynthesis / Q000235:genetics*; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D000074011:Peptide Transporter 1; D012333:RNA, Messenger; D027981:Symporters
keywords: Intestine; Japanese eel; Nutrient absorption; PEPT1; Peptide transporter 1; day(s) post hatching; dph; mRNA expression; peptide transporter 1