title: Characterisation of apoptosis in myb-transformed hematopoietic cell (MTHC-A) lines: TNF-α-induced apoptosis and prevention by cAMP.
abstract: Myb-transformed haematopoietic cell (MTHC) lines have been developed and clones selected based on their ability to respond to tumor necrosis factor (TNF)-α. MTHC-A cells underwent apoptosis in response to TNF-α (MTHC-A). The apoptotic effect of TNF-α in MTHC-A was mimicked by a specific inhibitor of protein kinase A (PKI 5-24) and by the tyrosine kinase inhibitor genistein, suggesting that phosphorylation of tyrosine and PKA activity were important in protecting MTHC from apoptosis. Agents that elevate intracellular levels of cAMP, e.g. cholera toxin and dibuteryl cAMP, protected MTHC-A from the apoptotic effects of TNF-α, and also reduced the apoptotic effects of PKI and genistein. MTHC-A thus provides a useful model for investigating the role of TNF-mediated apoptosis in regulation of the myeloid lineage of cells.
authors.affiliation: Department of Obstetrics and Gynecology, Dokkyo Medical University, Mibu, Tochigi, Japan. kenjis@jcom.home.ne.jp
authors.forename: Kenji
authors.initials: K
authors.lastname: Sorimachi
authors.forename: Paul
authors.initials: P
authors.lastname: Waring
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Hapel
authors.forename: Ichio
authors.initials: I
authors.lastname: Fukasawa
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kaneko
authors.forename: Nobuhide
authors.initials: N
authors.lastname: Masawa
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology; D015479:Leukemia, Myelomonocytic, Acute / Q000235:genetics / Q000378:metabolism; D051379:Mice; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D047428:Protein Kinase Inhibitors; D020598:Proto-Oncogene Proteins c-myb; D014409:Tumor Necrosis Factor-alpha; D000242:Cyclic AMP