title: Glucose and calcium ions may modulate the efficiency of bovine β-casomorphin-7 permeability through a monolayer of Caco-2 cells.
abstract: Milk and dairy products provide a lot of valuable nutritive elements. They are also sources of biologically active peptides, including β-casomorphins that manifest the properties of morphine. An activity of DPPIV seems to be most crucial factor decreasing the efficiency of the β-casomorphin-7 (BCM7) transport. The increase of BCM7 concentration in blood may intensify symptoms of apparent life threatening events (ALTE), autism, schizophrenia, and allergy. This study aimed at identifying the influence of several selected substances on a transport efficiency of bovine BCM7 through an intestinal monolayer in a Caco-2 cell model system. Applying the ELISA method, the permeability coefficient of BCM7 through the Caco-2 monolayer was calculated. TEER values were used to evaluate the integrity of Caco-2 cell monolayers. An increase of glucose and Ca(2+) concentrations in the culture medium was accompanied by an increase of the BCM7 transport efficiency. The lowest permeability coefficients of BCM7 were observed for the membranes with high electrical resistances. The transport was enhanced in the presence of milk infant formulas, whereas no changes were observed when using μ-opioid receptor antagonist (casoxin-6). The results may be useful in understanding the pathogenesis of inflammation and food allergy in infants.
authors.affiliation: Department of Biochemistry, Faculty of Biology & Biotechnology, University of Warmia and Mazury, ul. Oczapowskiego 1A, 10-719 Olsztyn, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Jarmołowska
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Teodorowicz
authors.forename: Ewa
authors.initials: E
authors.lastname: Fiedorowicz
authors.forename: Edyta
authors.initials: E
authors.lastname: Sienkiewicz-Szłapka
authors.forename: Michał
authors.initials: M
authors.lastname: Matysiewicz
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Kostyra
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D002463:Cell Membrane Permeability; D004723:Endorphins / Q000493:pharmacokinetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D010446:Peptide Fragments / Q000493:pharmacokinetics*
chemical_list: D004723:Endorphins; D010446:Peptide Fragments; C041128:beta-casomorphin 7; D005947:Glucose; D002118:Calcium
keywords: Caco-2 cells monolayer; Food allergy; Infant formula; Permeability coefficient; β-Casomorphin-7