title: Sedimentation velocity analytical ultracentrifugation in hydrogenated and deuterated solvents for the characterization of membrane proteins.
abstract: This chapter is a step-by-step protocol for setting up, realizing, and analyzing sedimentation velocity experiments in hydrogenated and deuterated solvents, in the context of the characterization of membrane protein, in terms of homogeneity, association state, and amount of bound detergent, based on a real case study of the membrane protein BmrA solubilized in n-Dodecyl-β-D-Maltopyranoside) detergent.
authors.affiliation: Institut de Biologie Structurale, CEA, Grenoble, France.
authors.forename: Aline
authors.initials: A
authors.lastname: Le Roy
authors.forename: Hugues
authors.initials: H
authors.lastname: Nury
authors.forename: Benjamin
authors.initials: B
authors.lastname: Wiseman
authors.forename: Jonathan
authors.initials: J
authors.lastname: Sarwan
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Jault
authors.forename: Christine
authors.initials: C
authors.lastname: Ebel
mesh_terms: D002499:Centrifugation, Density Gradient / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*
chemical_list: D008565:Membrane Proteins; D012997:Solvents