title: [Effects of methyl jasmonate on accumulation and release of main tropane alkaloids in liquid cultures of Datura stramonium hairy root].
abstract: OBJECTIVE
  To study the effects of methyl jasmonate (MJ) on the accumulation and release of main secondary metabolites i. e. scopolamine and hyoscyamine in liquid cultures of Datura stramonium hairy roots.
  
  
  METHODS
  After 18 days liquid culture of D. stramonium hairy roots induced by agrobacterium rhizogenes C58C1, the chemical elicitor methyl jasmonate was added into 1/2 MS liquid cultures and scopolamine and hyoscyamine on the day 0, 3, 6, 9 and 12, after dealing with MJ, was determined by HPLC.
  
  
  RESULTS
  After dealing with MJ on the day 3, 6, 9 and 12,the concentration of scopolamine reached to 0.419, 0.439, 0.431, 0.374 mg x g(-1), respectively, the increase of scopolamine were 1.36, 1.42, 1.17 and 1.12 fold higher than that of the control, respectively. And hyoscyamine reached 1.493, 0.817, 0.723 and 0.698 mg x g(-1), respectively, the increase of hyoscyamine were 2.28, 1.11, 0.63 and 0.70 fold higher than that of the control, respectively.
  
  
  CONCLUSIONS
  MJ could stimulate the accumulation of scopolamine and hyoscyamine (3,6 d) in D. stramonium hairy root and have released them into the culture medium.
authors.affiliation: School of Life Sciense, Southwest University/Key Laboratory of Eco-environments in Three Gorges Reservoir Region, MOE, Chongqing 400715, China. sunjiwei-2008@163.com
authors.forename: Ji-Wei
authors.initials: JW
authors.lastname: Sun
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Feng-Ying
authors.initials: FY
authors.lastname: Wang
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Sun
authors.forename: Min
authors.initials: M
authors.lastname: Sun
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000470:Alkaloids / Q000032:analysis / Q000378:metabolism*; D018929:Cell Culture Techniques; D003517:Cyclopentanes / Q000494:pharmacology*; D013286:Datura stramonium / Q000737:chemistry / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D054883:Oxylipins / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D014326:Tropanes / Q000032:analysis / Q000378:metabolism*
chemical_list: D000085:Acetates; D000470:Alkaloids; D003517:Cyclopentanes; D054883:Oxylipins; D014326:Tropanes; C072239:methyl jasmonate