title: Synthesis and enzymatic evaluation of phosphoramidon and its β anomer: Anomerization of α-l-rhamnose triacetate upon phosphitylation.
abstract: A novel and efficient strategy for the synthesis of phosphoramidon and its β anomer has been developed by manipulating the anomerization of α-l-rhamnose triacetate upon phosphitylation. The experimental results suggest that proton transfer, bond rotation, and N atom are the key factors for the anomerization. The determined Ki and Kd values establish that phosphoramidon prepared by this method possesses excellent biological activity, and indicate that the contacts of rhamnose moiety with the enzyme have limited contribution to the binding.
authors.affiliation: Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, 605 Fenglin Avenue, Nanchang, Jiangxi 330013, PR China. Electronic address: sunqi96@tsinghua.org.cn.
authors.forename: Qi
authors.initials: Q
authors.lastname: Sun
authors.forename: Qingkun
authors.initials: Q
authors.lastname: Yang
authors.forename: Shanshan
authors.initials: S
authors.lastname: Gong
authors.forename: Quanlei
authors.initials: Q
authors.lastname: Fu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xiao
mesh_terms: D006020:Glycopeptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011485:Protein Binding; D011522:Protons; D012210:Rhamnose / Q000737:chemistry*; D013237:Stereoisomerism; D013696:Temperature; D013820:Thermolysin / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D006020:Glycopeptides; D011522:Protons; D013820:Thermolysin; D012210:Rhamnose; C008890:phosphoramidon
keywords: Anomerization; Glycopeptide; Phosphoramidate; Phosphoramidite; Phosphoramidon