title: Growth Inhibitory Effect of (E)-2,4-bis(p-hydroxyphenyl)-2-Butenal Diacetate through Induction of Apoptotic Cell Death by Increasing DR3 Expression in Human Lung Cancer Cells.
abstract: The Maillard Reaction Products (MRPs) are chemical compounds which have been known to be effective in chemoprevention. Death receptors (DR) play a central role in directing apoptosis in several cancer cells. In our previous study, we demonstrated that (E)-2,4-bis(p-hydroxyphenyl)-2-butenal, a MRP product, inhibited human colon cancer cell growth by inducing apoptosis via nuclear factor-κB (NF-κB) inactivation and G2/M phase cell cycle arrest. In this study, (E)-2,4-bis(p-hydroxyphenyl)-2-butenal diacetate, a new (E)-2,4-bis(p-hydroxyphenyl)-2-butenal derivative, was synthesized to improve their solubility and stability in water and then evaluated against NCI-H460 and A549 human lung cancer cells. (E)-2,4-bis(p-hydroxyphenyl)-2-butenal diacetate reduced the viability in both cell lines in a time and dose-dependent manner. We also found that (E)-2,4-bis(p-hydroxyphenyl)-2-butenal diacetate increased apoptotic cell death through the upregulation of the expression of death receptor (DR)-3 and DR6 in both lung cancer cell lines. In addition to this, the transfection of DR3 siRNA diminished the growth inhibitory and apoptosis inducing effect of (E)-2,4-bis(p-hydroxyphenyl)-2-butenal diacetate on lung cancer cells, however these effects of (E)-2,4-bis(p-hydroxyphenyl)-2-butenal diacetate was not changed by DR6 siRNA. These results indicated that (E)-2,4-bis(p-hydroxyphenyl)-2-butenal diacetate inhibits human lung cancer cell growth via increasing apoptotic cell death by upregulation of the expression of DR3.
authors.affiliation: Department of Food Science & Technology, Korea National University of Transportation, Jeungpyeong 368-701, Republic of Korea.
authors.forename: Ung-Soo
authors.initials: US
authors.lastname: Lee
authors.forename: Jung Ok
authors.initials: JO
authors.lastname: Ban
authors.forename: Eung Tae
authors.initials: ET
authors.lastname: Yeon
authors.forename: Hee Pom
authors.initials: HP
authors.lastname: Lee
authors.forename: Venkatareddy
authors.initials: V
authors.lastname: Udumula
authors.forename: Young Wan
authors.initials: YW
authors.lastname: Ham
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
keywords: (E)-2; 4-bis(p-hydroxyphenyl)-2-butenal diacetate; Apoptosis; DR6; Human lung cancer cells