title: A C-type lectin could selectively facilitate bacteria clearance in red swamp crayfish, Procambarus clarkii.
abstract: C-type lectins function as pattern recognition receptors and play important roles in the innate immune system of crustaceans. In this study, we reported a new CTL gene (designated as PcLec5) from red swamp crayfish, Procambarus clarkii. PcLec5 was mainly distributed in hepatopancreas, gills and intestine, and the PcLec5 transcripts were up-regulated in all the three tissues after challenge with bacteria Vibrio anguillarum. For further functional analyses, PcLec5 was recombinantly expressed in Escherichia coli and anti-PcLec5 polyclonal antiserum was prepared. The results of bacteria binding assay revealed that PcLec5 could selectively bind to 5 of 9 kinds of bacteria we used and had a tendency to bind to Gram-negative bacteria. Sugar binding assay showed that PcLec5 could bind to peptidoglycan, lipoteichoic acid and lipopolysaccharide, with the highest affinity to LPS. Furthermore, bacteria-clearance experiment showed PcLec5 could selectively facilitate the clearance of injected bacteria in crayfish, and the bacteria-clearance facilitating spectrum of PcLec5 was totally in agreement with its bacteria binding spectrum. These results suggested PcLec5 function as a pattern recognition receptor in crayfish immune system and had certain selectivity on bacteria pathogens.
authors.affiliation: School of Life Science, Henan Normal University, Xinxiang, Henan 453002, China. Electronic address: hzz78@mail.missouri.edu.
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Ren
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Zhang
authors.forename: Ke-Ke
authors.initials: KK
authors.lastname: Wang
authors.forename: Jin-Xing
authors.initials: JX
authors.lastname: Wang
mesh_terms: D000818:Animals; D003400:Astacoidea / Q000276:immunology* / Q000382:microbiology*; D001483:Base Sequence; D015153:Blotting, Western; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli; D005786:Gene Expression Regulation / Q000276:immunology*; D005880:Gills / Q000378:metabolism; D043143:Hepatopancreas / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D037181:Lectins, C-Type / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013682:Teichoic Acids / Q000378:metabolism; D014733:Vibrio / Q000276:immunology*
chemical_list: D018076:DNA, Complementary; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D010457:Peptidoglycan; D013682:Teichoic Acids; C009900:lipoteichoic acid
keywords: Bacteria clearance; C-type lectin; Immunity; Pattern recognition receptor; Procambarus clarkii