title: [Effect of total ginsenoside on content of protein and activity of digestive enzyme of Mythimna separata larvae].
abstract: OBJECTIVE
  This study aims to reveal the effect of total ginsenoside on the protein content and digestive enzyme activities of 4th-instar Mythimna separata larvae, including alpha-amylase and cellulose, and explore the ecological function of total ginsenoside.
  
  
  METHODS
  While simulating natural growing condition indoors, 4th-instar M. separata larvae were fed by poison leaf disk method. The protein content was tested by Lowry Protein Assay Kit method, the activity of alpha-amylase was measured by dinitrosalicylic acid test, and the activity of cellulase was determined by the filter paper method.
  
  
  RESULTS
  The total ginsenoside could reduce the content of protein of 4th-instar M. separata larvae significantly, and the activity of digestive enzyme, including alpha-amylase and cellulase. The protein content, alpha-amylase and cellulase activity of treatments were obviously lower than that of the control. Inhibition ratio of alpha-amylase and cellulase activity was positively correlated with total ginsenoside concentration: i. e. 20 g x L(-1) > 10 g x L(-1) > 5 g x L(-1).
  
  
  CONCLUSIONS
  The results suggest that the inhibition effect of total ginsenoside on protein content and digestive enzymes may be one of the causes to antifeedant and dysplasia of M. separata larvae.
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agricultural University, Ginseng Engineering Research Centre of Jilin, Changchun 130018, China. choohcho@163.com
authors.forename: Shi-Qiang
authors.initials: SQ
authors.lastname: Tan
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Zhang
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Xu
authors.forename: Lian-Xue
authors.initials: LX
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004063:Digestion; D036145:Ginsenosides / Q000494:pharmacology*; D019476:Insect Proteins / Q000378:metabolism*; D007814:Larva / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D009036:Moths / Q000187:drug effects* / Q000201:enzymology* / Q000254:growth & development
chemical_list: D036145:Ginsenosides; D019476:Insect Proteins