title: Zunongwangia atlantica sp. nov., isolated from deep-sea water.
abstract: A taxonomic study was carried out on strain 22II14-10F7(T), which was isolated from the deep-sea water of the Atlantic Ocean with oil-degrading enrichment. The bacterium was Gram-stain-negative, oxidase- and catalase-positive and rod-shaped. Growth was observed at salinities from 0.5 to 15 % and at temperatures from 4 to 37 °C; it was unable to hydrolyse Tween 40, 80 or gelatin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II14-10F7(T) represented a member of the genus Zunongwangia, with highest sequence similarity of 97.3 % to Zunongwangia profunda SM-A87(T), while the similarities to other species were all below 94.0 %. The DNA-DNA hybridization estimate of the similarity between strain 22II14-10F7(T) and Z. profunda SM-A87(T) was 27.20±2.43 % according to their genome sequences. The principal fatty acids were iso-C15 : 0, anteiso-C15 : 0 , iso-C15 : 1 G, iso-C17 : 0 3-OH, summed feature 3 (C16 : 1ω7c/ω6c) and summed feature 9 (iso-C17 : 1ω9c or C16 : 0 10-methyl). The G+C content of the chromosomal DNA was 35.5 mol%. The major respiratory quinone was determined to be MK-6. Phosphatidylethanolamine (PE), two aminolipids (AL1 and AL2) and five unknown lipids (L1-L5) were present. The combined genotypic and phenotypic data show that strain 22II14-10F7(T) represents a novel species of the genus Zunongwangia, for which the name Zunongwangia atlantica sp. nov. is proposed, with the type strain 22II14-10F7(T) ( = CGMCC1.12470(T) = LMG 27421(T) = MCCC 1A06481(T)).
authors.affiliation: Life Science College, Xiamen University, Xiamen 361005, PR China.|State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Shao
authors.affiliation: Life Science College, Xiamen University, Xiamen 361005, PR China.|State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Lai
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.
authors.forename: Xiupian
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.
authors.forename: Fengqin
authors.initials: F
authors.lastname: Sun
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Du
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Li
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.
authors.forename: Zongze
authors.initials: Z
authors.lastname: Shao
mesh_terms: D001267:Atlantic Ocean; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6