title: Induction of somatic embryogenesis in endangered butterfly ginger Hedychium coronarium J. Koenig.
abstract: An efficient protocol has been developed for regeneration of complete plants through somatic embryogenesis in H. coronarium. Creamish white, pale yellow and brown calli were obtained on MS medium supplemented with different concentrations of auxins [2, 4-Dichlorophenoxy acetic acid (2, 4-D), Indole-3 acetic acid (IAA) and 1-Naphthylacetic acid (NAA)] after 4 weeks. Creamy white calli developed on 0.5 mg L(-1) 2, 4-D turned embryogenic when subcultured on basal medium and produced small globular somatic embryos after 6 weeks. Further growth of somatic embryos required their transfer to medium containing 6-benzylaminopurine (BAP) or kinetin (KN). BAP was more effective than KN in promoting shoot proliferation. Maximum shoot length was obtained with 0.5 mg L(-1) BAP whereas maximum shoot number was obtained with 1.0 mg L(-1) BAP. The plantlets thus formed were successfully hardened, and transferred to sand-soil and farm yard manure (1:1:1) with 95% survival.
authors.affiliation: Plant Tissue Culture Laboratory, Department of P.G. Studies and Research in Biological Science, R. D University, Jabalpur 482 001, India.
authors.forename: Manju
authors.initials: M
authors.lastname: Verma
authors.forename: Y K
authors.initials: YK
authors.lastname: Bansal
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology*; D001593:Benzyl Compounds; D007210:Indoleacetic Acids / Q000494:pharmacology; D007701:Kinetin / Q000494:pharmacology; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000254:growth & development; D058439:Plant Somatic Embryogenesis Techniques; D011687:Purines; D030521:Zingiberaceae / Q000187:drug effects / Q000196:embryology* / Q000254:growth & development
chemical_list: D001593:Benzyl Compounds; D007210:Indoleacetic Acids; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; D015084:2,4-Dichlorophenoxyacetic Acid; C034182:1-naphthaleneacetic acid; C030737:indoleacetic acid; C480551:benzylaminopurine; D007701:Kinetin