title: Inhibitory effect of sanguinarine on PKC-CPI-17 pathway mediating by muscarinic receptors in dispersed intestinal smooth muscle cells.
abstract: This study investigated the inhibitory effects of sanguinarine (SA) on PKC-CPI-17 pathway in rat intestinal smooth muscle cells (ISMC). Previous studies indicate that the inhibitory effects of SA on ISMC contraction are possibly mediated by the Ca(2+) influx. ISMC was treated with 1 μM SA for 24h remarkably inhibited the mRNA expression of m2 and m3 receptors. ISMC treated with 1 or 3 μM SA for 30 min significantly decreased the mRNA expression of PKC-δ, PKC-ε, PKC-η, and CPI-17. 1 μM SA could markedly inhibit carbachol (CCh)-mediated increase PKC-δ, PKC-η, and CPI-17 mRNA but had no effect in PKC-ε.Treatment of ISMC with SA (1 μM, 30 min) caused a decrease in protein expression of PKC-δ. However, the expression of CPI-17 was significantly inhibited in a time-dependent manner. These results demonstrate that the inhibitory effect of SA is coupled with alteration of PKC-mediated signal transduction and intracellular Ca(2+) concentration.
authors.affiliation: College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan 410128, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Guohua
authors.initials: G
authors.lastname: Yin
authors.forename: Chun-Hong
authors.initials: CH
authors.lastname: Yu
authors.forename: Yueshang
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhi-Liang
authors.initials: ZL
authors.lastname: Sun
mesh_terms: D000818:Animals; D053119:Benzophenanthridines / Q000494:pharmacology*; D015153:Blotting, Western; D002118:Calcium / Q000032:analysis; D007422:Intestines / Q000187:drug effects; D007546:Isoquinolines / Q000494:pharmacology*; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000737:chemistry / Q000187:drug effects*; D010749:Phosphoprotein Phosphatases / Q000187:drug effects*; D011493:Protein Kinase C / Q000187:drug effects*; D051745:Protein Kinase C-delta / Q000187:drug effects; D051381:Rats; D011976:Receptors, Muscarinic / Q000187:drug effects*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D053119:Benzophenanthridines; D007546:Isoquinolines; D011976:Receptors, Muscarinic; C005705:sanguinarine; D011493:Protein Kinase C; D051745:Protein Kinase C-delta; D010749:Phosphoprotein Phosphatases; D002118:Calcium
keywords: Ca(2+); Intestinal smooth muscle cells (ISMC); M receptors; PKC-CPI-17 pathway; Sanguinarine (SA)