title: A chemical quenching- and physical blocking-based method to minimize process-mediated aggregation of antibody-crosslinked nanoparticles for imaging application.
abstract: Critical limitation of nanoparticles (NP) is their aggregation after functionalisation and antibody cross-linking. We analysed the cause of this aggregation with respect to functionalities (carboxyls and amines) on the NP surface. We have devised a low cost novel method to reduce such aggregations during protein cross-linking and validated it by probing the platelet surface with platelet surface-specific anti-CD41 antibody conjugated NPs.
authors.affiliation: School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.
authors.forename: Chandra K
authors.initials: CK
authors.lastname: Dixit
authors.forename: Shibsekhar
authors.initials: S
authors.lastname: Roy
authors.forename: Conor
authors.initials: C
authors.lastname: Byrne
authors.forename: Richard
authors.initials: R
authors.lastname: O'Kennedy
authors.forename: Colette
authors.initials: C
authors.lastname: McDonagh
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism; D002417:Cattle; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism*; D040201:Platelet Membrane Glycoprotein IIb / Q000378:metabolism*
chemical_list: D000906:Antibodies; D040201:Platelet Membrane Glycoprotein IIb