title: [Analysis of three wheat cytoplasmic male sterile lines mitochondrial DNA by AFLP].
abstract: Cytoplasmic male sterility is an important way to utilize wheat heterosis. The purpose of thisstudy was to identify cytoplasmic type of three wheat male sterile lines. Amplified fragment length polymorphism (AFLP) marker technique was used to analyze the wheat mitochondrial DNA. We isolated mitochondria by differential centrifugation and density gradient ultracentrifugation. The results show that the extracted mitochondrial DNA was pure. It was suitable for PCR and genetic analysis. We got 4 pairs of specific primers from 64 primers combinations. Primer E1/M7 amplified 3 specific fragments in ms(Kots)-90-110. Primer E4/M2 generated 2 specific fragments in ms(Ven)-90-110. Primer E7/M6 amplified 2 specific fragments in ms(S)-90-110. Primer E6/M4 produced 2 specific fragments in ms(Kots)-90-110. Four specific primers could be used to identify three cytoplasmic types of Aegilops kotschyi, Ae. ventricosa and Triticum spelta. It provided the molecular basis to further study the mechanism of wheat cytoplasmic male sterility.
authors.affiliation: College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Qidi
authors.initials: Q
authors.lastname: Zhu
authors.forename: Xinbo
authors.initials: X
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Ejaz
authors.forename: Gaisheng
authors.initials: G
authors.lastname: Zhang
authors.forename: Huixue
authors.initials: H
authors.lastname: Che
authors.forename: Shuping
authors.initials: S
authors.lastname: Wang
authors.forename: Qilu
authors.initials: Q
authors.lastname: Song
authors.forename: Shuling
authors.initials: S
authors.lastname: Yang
authors.forename: Longyu
authors.initials: L
authors.lastname: Zhang
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D003593:Cytoplasm / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D020869:Gene Expression Profiling; D005838:Genotype; D051479:Plant Infertility / Q000235:genetics*; D014908:Triticum / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D018744:DNA, Plant