title: Long-lived luminogenic probe for detection of RNA in a crude solution of living bacterial cells.
abstract: A pre-type sensitizer for a lanthanide complex on an oligonucleotide was successfully converted to a perfect final structure in a target DNA/RNA-templated reaction, without any chemical reagent or enzyme, under neutral conditions. The final form of the lanthanide-oligonucleotide provided a long-lived luminescence signal, appropriate for time-gated luminescence analysis and signal amplification. Target DNA/RNA-assisted time-gated luminescence analysis is a powerful tool for elimination of autofluorescence and detection of target RNA in living bacterial cells.
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN , 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Hisao
authors.initials: H
authors.lastname: Saneyoshi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Abe
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D056831:Coordination Complexes / Q000737:chemistry; D004247:DNA / Q000032:analysis / Q000235:genetics; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry*; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D057230:Limit of Detection; D008163:Luminescent Measurements; D015394:Molecular Structure; D012313:RNA / Q000032:analysis* / Q000235:genetics
chemical_list: D056831:Coordination Complexes; D005456:Fluorescent Dyes; D028581:Lanthanoid Series Elements; D012313:RNA; D004247:DNA