title: Single channel analysis of membrane proteins in artificial bilayer membranes.
abstract: The planar lipid bilayer technique is a powerful experimental approach for electrical single channel recordings of pore-forming membrane proteins in a chemically well-defined and easily modifiable environment. Here we provide a general survey of the basic materials and procedures required to set up a robust bilayer system and perform electrophysiological single channel recordings of reconstituted proteins suitable for the in-depth characterization of their functional properties.
authors.affiliation: Biophysics, Department of Biology/Chemistry, University of Osnabrueck, Osnabrueck, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Bartsch
authors.forename: Anke
authors.initials: A
authors.lastname: Harsman
authors.forename: Richard
authors.initials: R
authors.lastname: Wagner
mesh_terms: D004553:Electric Conductivity; D004566:Electrodes; D008051:Lipid Bilayers / Q000737:chemistry*; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D008565:Membrane Proteins