title: Effects of resveratrol on obesity-related inflammation markers in adipose tissue of genetically obese rats.
abstract: OBJECTIVE
  The aim of this study was to examine whether resveratrol might represent a promising therapeutic tool with which to combat adipose tissue chronic inflammation in a model of genetic obesity and to link its anti-inflammatory activity with its effect on body fat reduction.
  
  
  METHODS
  Twenty 6-wk-old male Zucker (fa/fa) rats were randomly distributed into two experimental groups. Resveratrol (RSV) was given orally (15 mg/kg body weight/d in RSV group) by means of an orogastric catheter for 6 wk. Enzyme activities were measured spectrophotometrically or fluorimetrically. Gene and protein expressions were analyzed by reverse transcriptase polymerase chain reaction and Western blot respectively. Cytokine concentrations and the activity of nuclear factor κ-light-chain-enhancer of activated β cells (NF-κB) were measured by using commercial kits.
  
  
  RESULTS
  RSV reduced the weight of internal adipose tissues. In epididymal depot glucose-6P-dehydrogenase, acetyl-CoA carboxylase activities, as well as lipoprotein lipase expression and activity were reduced by RSV. The expression of hormone-sensitive lipase was increased, and that of the cluster of differentiation 36 was reduced. Serum concentrations of tumor necrosis factor-α, monocyte chemoattractant protein 1, and C-reactive protein were lower in the RSV-treated group than in the control group. Protein expression of interleukin-6 and the activity of NF-κB, were decreased by RSV.
  
  
  CONCLUSIONS
  The present results provide evidence that fatty acid uptake and lipolysis are metabolic pathways involved in the response of adipose tissue to RSV. This polyphenol modulates plasma cytokine levels partially by reducing macrophage infiltration in adipose tissue and inhibiting NF-κB activity.
authors.affiliation: Nutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country, Vitoria, Spain; CIBERobn Physiopathology of Obesity and Nutrition, Institute of Health Carlos III, Madrid, Spain.
authors.forename: Saioa
authors.initials: S
authors.lastname: Gómez-Zorita
authors.forename: Alfredo
authors.initials: A
authors.lastname: Fernández-Quintela
authors.forename: Arrate
authors.initials: A
authors.lastname: Lasa
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Hijona
authors.forename: Luis
authors.initials: L
authors.lastname: Bujanda
authors.forename: María P
authors.initials: MP
authors.lastname: Portillo
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D052242:Adiponectin / Q000097:blood; D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002097:C-Reactive Protein / Q000378:metabolism; D018932:Chemokine CCL2 / Q000097:blood; D005227:Fatty Acids / Q000008:administration & dosage / Q000493:pharmacokinetics; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007249:Inflammation / Q000097:blood / Q000209:etiology / Q000378:metabolism*; D015850:Interleukin-6 / Q000097:blood; D008066:Lipolysis / Q000187:drug effects; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D009765:Obesity / Q000150:complications / Q000378:metabolism*; D051381:Rats; D011924:Rats, Zucker; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D052242:Adiponectin; C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D005227:Fatty Acids; D015850:Interleukin-6; D016328:NF-kappa B; D013267:Stilbenes; D014409:Tumor Necrosis Factor-alpha; D002097:C-Reactive Protein; D005954:Glucosephosphate Dehydrogenase; D000103:Acetyl-CoA Carboxylase; D000077185:Resveratrol
keywords: Adipose tissue; Inflammation; Lipases; Lipogenesis; Resveratrol; Zucker rat