title: Global profiling of stimulus-induced polyadenylation in cells using a poly(A) trap.
abstract: Polyadenylation of mRNA leads to increased protein expression in response to diverse stimuli, but it is difficult to identify mRNAs that become polyadenylated in living cells. Here we describe a click chemistry-compatible nucleoside analog that is selectively incorporated into poly(A) tails of transcripts in cells. Next-generation sequencing of labeled mRNAs enables a transcriptome-wide profile of polyadenylation and provides insights into the mRNA sequence elements that are correlated with polyadenylation.
authors.affiliation: Department of Pharmacology, Weill Medical College, Cornell University, New York, New York, USA.
authors.forename: Dusica
authors.initials: D
authors.lastname: Curanovic
authors.forename: Michael
authors.initials: M
authors.lastname: Cohen
authors.forename: Irtisha
authors.initials: I
authors.lastname: Singh
authors.forename: Christopher E
authors.initials: CE
authors.lastname: Slagle
authors.forename: Christina S
authors.initials: CS
authors.lastname: Leslie
authors.forename: Samie R
authors.initials: SR
authors.lastname: Jaffrey
mesh_terms: D057930:Click Chemistry; D011061:Poly A / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D026723:Polyadenylation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D011061:Poly A