title: Optimization of insect cell based protein production processes - online monitoring, expression systems, scale up.
abstract: Due to the increasing use of insect cell based expression systems in research and industrial recombinant protein production, the development of efficient and reproducible production processes remains a challenging task. In this context, the application of online monitoring techniques is intended to ensure high and reproducible product qualities already during the early phases of process development. In the following chapter, the most common transient and stable insect cell based expression systems are briefly introduced. Novel applications of insect cell based expression systems for the production of insect derived antimicrobial peptides/proteins (AMPs) are discussed using the example of G. mellonella derived gloverin. Suitable in situ sensor techniques for insect cell culture monitoring in disposable and common bioreactor systems are outlined with respect to optical and capacitive sensor concepts. Since scale up of production processes is one of the most critical steps in process development, a conclusive overview is given about scale up aspects for industrial insect cell culture processes.
authors.affiliation: Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Giessen, Germany.
authors.forename: Damir
authors.initials: D
authors.lastname: Druzinec
authors.forename: Denise
authors.initials: D
authors.lastname: Salzig
authors.forename: Alexander
authors.initials: A
authors.lastname: Brix
authors.forename: Matthias
authors.initials: M
authors.lastname: Kraume
authors.forename: Andreas
authors.initials: A
authors.lastname: Vilcinskas
authors.forename: Christian
authors.initials: C
authors.lastname: Kollewe
authors.forename: Peter
authors.initials: P
authors.lastname: Czermak
mesh_terms: D000818:Animals; D061249:Batch Cell Culture Techniques / Q000379:methods*; D019149:Bioreactors; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000502:physiology; D036341:Intercellular Signaling Peptides and Proteins; D007915:Lepidoptera / Q000166:cytology / Q000502:physiology*; D008991:Monitoring, Physiologic / Q000379:methods*; D009862:Online Systems; D010865:Pilot Projects; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins; D011994:Recombinant Proteins; C107617:gloverin