title: Defining a substrate-binding model of a polysialyltransferase.
abstract: Highly disciplined transfers: Polysialyltransferases are important enzymes responsible for the biosynthesis of α-linked polysialic acids. We used a multidisciplinary approach, and propose the first substrate-binding model for a bacterial polysialyltransferase. Furthermore, we identify key amino acid residues involved in catalysis.
authors.affiliation: Institute for Cellular Chemistry, Hannover Medical School, Carl-Neuberg Strasse 1, 30625 Hannover (Germany).
authors.forename: Friedrich
authors.initials: F
authors.lastname: Freiberger
authors.forename: Raphael
authors.initials: R
authors.lastname: Böhm
authors.forename: David
authors.initials: D
authors.lastname: Schwarzer
authors.forename: Rita
authors.initials: R
authors.lastname: Gerardy-Schahn
authors.forename: Thomas
authors.initials: T
authors.lastname: Haselhorst
authors.forename: Mark
authors.initials: M
authors.lastname: von Itzstein
mesh_terms: D019295:Computational Biology; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009345:Neisseria meningitidis / Q000201:enzymology; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D017433:Protein Structure, Secondary; D012799:Sialyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D012799:Sialyltransferases
keywords: NMR spectroscopy; enzyme models; glycosyltransferases; polysialic acids; sialic acids