title: Effect of biodiesel addition on microbial community structure in a simulated fuel storage system.
abstract: Understanding changes in microbial structure due to biodiesel storage is important both for protecting integrity of storage systems and fuel quality management. In this work a simulated storage system was used to study the effect of biodiesel (0%, 25%, 50%, 75% and 100%) on a microbial population, which was followed by community level physiological profiling (CLPP), 16s rDNA analysis and plating in selective media. Results proved that structure and functionality were affected by biodiesel. CLPP showed at least three populations: one corresponding to diesel, one to biodiesel and one to blends of diesel and biodiesel. Analysis of 16s rDNA revealed that microbial composition was different for populations growing in diesel and biodiesel. Genera identified are known for degradation of hydrocarbons and emulsifier production. Maximum growth was obtained in biodiesel; however, microbial counts in standard media were lower for this samples. Acidification of culture media was observed at high biodiesel concentration.
authors.affiliation: Chemical and Biochemical Engineering, University of Western Ontario, 1151 Richmond Street, London, Ontario N6A 3K7, Canada.
authors.forename: Juan-Manuel
authors.initials: JM
authors.lastname: Restrepo-Flórez
authors.affiliation: Chemical and Biochemical Engineering, University of Western Ontario, 1151 Richmond Street, London, Ontario N6A 3K7, Canada. Electronic address: abassi@uwo.ca.
authors.forename: Amarjeet
authors.initials: A
authors.lastname: Bassi
authors.affiliation: Chemical and Biochemical Engineering, University of Western Ontario, 1151 Richmond Street, London, Ontario N6A 3K7, Canada.
authors.forename: Lars
authors.initials: L
authors.lastname: Rehmann
authors.affiliation: Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4L7, Canada.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Thompson
mesh_terms: D001483:Base Sequence; D056804:Biofuels; D015169:Colony Count, Microbial; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics; D064307:Microbiota; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D056804:Biofuels; D017931:DNA Primers; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: 16s rDNA; Biodiesel; CLPP; Microbial community structure