title: Molecular design for enhanced sensitivity of a FRET aptasensor built on the graphene oxide surface.
abstract: We designed a biomolecular probe for highly sensitive protein detection by modifying an aptamer with a DNA spacer. The spacer controls the distance between a fluorescence dye and a quencher, which is crucial for FRET-based sensors. We successfully demonstrated an improvement in the sensitivity of an on-chip graphene oxide aptasensor.
authors.affiliation: NTT Microsystem Integration Laboratories, NTT Corporation, 3-1 Morinosato Wakamiya, Atsugi, Kanagawa, Japan. ueno.yuko@lab.ntt.co.jp.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ueno
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Furukawa
authors.forename: Kota
authors.initials: K
authors.lastname: Matsuo
authors.forename: Suzuyo
authors.initials: S
authors.lastname: Inoue
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Hayashi
authors.forename: Hiroki
authors.initials: H
authors.lastname: Hibino
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010087:Oxides / Q000737:chemistry*; D011487:Protein Conformation; D013499:Surface Properties; D013917:Thrombin / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D010087:Oxides; D006108:Graphite; D013917:Thrombin