title: Inhibition of angiogenesis by a novel neutralizing antibody targeting human VEGFR-3.
abstract: Vascular endothelial growth factor receptor 3 (VEGFR-3) is a receptor for the vascular endothelial growth factor C and D (VEGF-C and D) and plays a critical role in the development of embryonic vascular system and regulation of tumor lymphangiogenesis. In this report, we generated a novel panel of 17 monoclonal antibodies (mAbs) against human VEGFR-3 and determined their ability to inhibit the proliferation of human erythroleukemia (HEL) cells and angiogenesis of chick embryo chorioallantoic membrane (CAM). Among these mAbs, BDD073 was demonstrated to inhibit the interaction of soluble VEGFR-3 with VEGF-D and the proliferation of HEL cells. Furthermore, in chick embryo CAM angiogenesis experiments, the angiogenesis induced by recombinant glutathione-S-transferase-VEGF-D was decreased in the presence of antibody BDD073. These data suggest that this novel neutralizing antibody against human VEGFR-3 could be a tool for the investigations into the biology of VEGFR-3, and potentially a reagent for blocking VEGF-D-induced angiogenesis and lymphogenesis.
authors.affiliation: The Second People's Hospital of Shenzhen; The First Affiliated Hospital of Shenzhen University; Shenzhen, PR China; Epithelial Cell Biology Research Center; School of Biomedical Sciences; Faculty of Medicine; The Chinese University of Hong Kong; Shatin, Hong Kong.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Immunology; Beijing Institute of Radiation Medicine; Beijing, P.R. China.
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Ding
authors.affiliation: Department of Immunology; Beijing Institute of Radiation Medicine; Beijing, P.R. China; Antibody Engineering Laboratory; Beijing Proteome Research Center; Beijing, P.R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Epithelial Cell Biology Research Center; School of Biomedical Sciences; Faculty of Medicine; The Chinese University of Hong Kong; Shatin, Hong Kong.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Immunology; Beijing Institute of Radiation Medicine; Beijing, P.R. China; Antibody Engineering Laboratory; Beijing Proteome Research Center; Beijing, P.R. China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Immunology; Beijing Institute of Radiation Medicine; Beijing, P.R. China; Antibody Engineering Laboratory; Beijing Proteome Research Center; Beijing, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Immunology; Beijing Institute of Radiation Medicine; Beijing, P.R. China; Antibody Engineering Laboratory; Beijing Proteome Research Center; Beijing, P.R. China.
authors.forename: Jianen
authors.initials: J
authors.lastname: Gao
authors.affiliation: The Second People's Hospital of Shenzhen; The First Affiliated Hospital of Shenzhen University; Shenzhen, PR China.
authors.forename: Xiaping
authors.initials: X
authors.lastname: Zhou
authors.affiliation: The Second People's Hospital of Shenzhen; The First Affiliated Hospital of Shenzhen University; Shenzhen, PR China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Department of Immunology; Beijing Institute of Radiation Medicine; Beijing, P.R. China; Antibody Engineering Laboratory; Beijing Proteome Research Center; Beijing, P.R. China.
authors.forename: Qihong
authors.initials: Q
authors.lastname: Sun
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D057134:Antibodies, Neutralizing / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology* / Q000627:therapeutic use; D015153:Blotting, Western; D002642:Chick Embryo; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009389:Neovascularization, Pathologic / Q000188:drug therapy; D011485:Protein Binding / Q000187:drug effects; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040321:Vascular Endothelial Growth Factor Receptor-3 / Q000378:metabolism*
chemical_list: D020533:Angiogenesis Inhibitors; D057134:Antibodies, Neutralizing; D011994:Recombinant Proteins; D040321:Vascular Endothelial Growth Factor Receptor-3
keywords: VEGF-D; VEGFR-3; angiogenesis; antibody