title: [The techniques to detect apoptosis of spermatozoids].
abstract: The article presents the classification and evaluation of techniques applied in documentation of apoptosis of spermatozoids. The main point of techniques detecting the changes in DNA of apoptosis spermatozoids (SCSA technique, TUNEL test and Comet assay) is revealed The annexin technique, the technique of detection of changes of cells transmembrane potential, the technique of detection of activity of caspase and technique of detection the degree of expression of proteins-regulators of apoptosis are discussed too. The advantages and shortcomings of these techniques are considered.
authors.forename: M V
authors.initials: MV
authors.lastname: Ploskonos
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D020552:Comet Assay; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D020287:In Situ Nick-End Labeling; D008297:Male; D013094:Spermatozoa / Q000166:cytology*
chemical_list: D004247:DNA