title: Molten globule of hemoglobin proceeds into aggregates and advanced glycated end products.
abstract: Conformational alterations of bovine hemoglobin (Hb) upon sequential addition of glyoxal over a range of 0-90% v/v were investigated. At 20% v/v glyoxal, molten globule (MG) state of Hb was observed by altered tryptophan fluorescence, high ANS binding, existence of intact heme, native-like secondary structure as depicted by far-UV circular dichroism (CD) and ATR-FTIR spectra as well as loss in tertiary structure as confirmed by near-UV CD spectra. In addition, size exclusion chromatography analysis depicted that MG state at 20% v/v glyoxal corresponded to expanded pre-dissociated dimers. Aggregates of Hb were detected at 70% v/v glyoxal. These aggregates of Hb had altered tryptophan environment, low ANS binding, exposed heme, increased β-sheet secondary structure, loss in tertiary structure, enhanced thioflavin T (ThT) fluorescence and red shifted Congo Red (CR) absorbance. On incubating Hb with 30% v/v glyoxal for 0-20 days, advanced glycation end products (AGEs) were detected on day 20. These AGEs were characterised by enhanced tryptophan fluorescence at 450 nm, exposure of heme, increase in intermolecular β-sheets, enhanced ThT fluorescence and red shift in CR absorbance. Comet assay revealed aggregates and AGEs to be genotoxic in nature. Scanning electron microscopy confirmed the amorphous structure of aggregates and branched fibrils of AGEs. The transformation of α-helix to β-sheet usually alters the normal protein to amyloidogenic resulting in a variety of protein conformational disorders such as diabetes, prion and Huntington's.
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India.
authors.forename: Afshin
authors.initials: A
authors.lastname: Iram
authors.forename: Tauqeer
authors.initials: T
authors.lastname: Alam
authors.forename: Javed M
authors.initials: JM
authors.lastname: Khan
authors.forename: Taqi A
authors.initials: TA
authors.lastname: Khan
authors.forename: Rizwan H
authors.initials: RH
authors.lastname: Khan
authors.forename: Aabgeena
authors.initials: A
authors.lastname: Naeem
mesh_terms: D000818:Animals; D052160:Benzothiazoles; D002417:Cattle; D002942:Circular Dichroism; D003224:Congo Red / Q000737:chemistry; D017127:Glycation End Products, Advanced / Q000737:chemistry*; D006031:Glycosylation; D006037:Glyoxal / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D013844:Thiazoles / Q000737:chemistry; D013997:Time Factors; D014364:Tryptophan / Q000737:chemistry
chemical_list: D052160:Benzothiazoles; D017127:Glycation End Products, Advanced; D006454:Hemoglobins; D013844:Thiazoles; C009462:thioflavin T; D003224:Congo Red; D006037:Glyoxal; D014364:Tryptophan