title: Nocardioides endophyticus sp. nov. and Nocardioides conyzicola sp. nov., isolated from herbaceous plant roots.
abstract: Two Gram-stain-positive, non-motile, non-spore-forming, rod-shaped actinobacterial strains were isolated from the surface-sterilized roots of mugwort (Artemisia princeps) and horse-weed (Conyza canadensis), and subjected to taxonomic characterization. 16S rRNA gene sequence analysis indicated that the isolates, designated MWE 3-5(T) and HWE 2-02(T), should be placed in the genus Nocardioides of the family Nocardioidaceae. The strains were closely related to Nocardioides hankookensis DS-30(T), which exhibited 16S rRNA gene sequence similarity values of 97.99 and 99.09 % with strains MWE 3-5(T) and HWE 2-02(T), respectively. The genome relatedness of N. hankookensis DS-30(T) with strain MWE 3-5(T) was 35.8 %, and that with strain HWE 2-02(T) was 36.4 %, whereas that between the two isolates was 43.2 %. Strains MWE 3-5(T) and HWE 2-02(T) possessed MK-8(H4) as the major isoprenoid quinone, and ll-diaminopimelic acid in the cell-wall peptidoglycan. The main fatty acids were iso-C16 : 0, iso-C17 : 0 and C18 : 1ω9c for strain MWE 3-5(T) and iso-C16 : 0, 10-methyl C18 : 0 and C18 : 1ω9c for strain HWE 2-02(T). Based on phenotypic, genotypic and phylogenetic studies, the following two novel species are proposed: Nocardioides endophyticus sp. nov. (type strain, MWE 3-5(T) = KCTC 29122(T) = JCM 18532(T)) and Nocardioides conyzicola sp. nov. (type strain, HWE 2-02(T) = KCTC 29121(T) = JCM 18531(T)).
authors.affiliation: Department of Microbiology and Molecular Biology, School of Bioscience and Biotechnology, Chungnam National University, 220 Gung-Dong, Yuseong, Daejeon 305-764, Republic of Korea.
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Han
authors.affiliation: Department of Microbiology and Molecular Biology, School of Bioscience and Biotechnology, Chungnam National University, 220 Gung-Dong, Yuseong, Daejeon 305-764, Republic of Korea.
authors.forename: Tae-Su
authors.initials: TS
authors.lastname: Kim
authors.affiliation: Department of Microbiology and Molecular Biology, School of Bioscience and Biotechnology, Chungnam National University, 220 Gung-Dong, Yuseong, Daejeon 305-764, Republic of Korea.
authors.forename: Yochan
authors.initials: Y
authors.lastname: Joung
authors.affiliation: Korea Research Institute of Bioscience & Biotechnology, 52 Oun-Dong, Yuseong, Daejeon 305-333, Republic of Korea.
authors.forename: Mi Na
authors.initials: MN
authors.lastname: Kim
authors.affiliation: Korea Research Institute of Bioscience & Biotechnology, 52 Oun-Dong, Yuseong, Daejeon 305-333, Republic of Korea.
authors.forename: Kee-Sun
authors.initials: KS
authors.lastname: Shin
authors.affiliation: Department of Microbiology and Immunology, Indiana University School of Medicine-Northwest, Gary, IN46408, USA.
authors.forename: Taeok
authors.initials: T
authors.lastname: Bae
authors.affiliation: Department of Microbiology and Molecular Biology, School of Bioscience and Biotechnology, Chungnam National University, 220 Gung-Dong, Yuseong, Daejeon 305-764, Republic of Korea.
authors.forename: Seung Bum
authors.initials: SB
authors.lastname: Kim
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019306:Artemisia / Q000382:microbiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D035401:Conyza / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8; D003960:Diaminopimelic Acid