title: Characterization of a glycyrrhizin-deficient strain of Glycyrrhiza uralensis.
abstract: A triterpene saponin, glucoglycyrrhizin, was isolated from a glycyrrhizin-deficient strain 83-555 of Glycyrrhiza uralensis (Leguminosae), and the structure was determined by chemical and spectral data to be 3-O-[β-D-glucopyranosyl-(1→2)-β-D-glucuronopyranosyl]-glycyrrhetinic acid. Since this saponin has a 2'-O-β-D-glucopyranosyl moiety instead of the 2'-O-β-D-glucuronopyranosyl moiety of glycyrrhizin, the glucuronidation of 3-O-β-D-glucuronopyranosyl-glycyrrhetinic acid leading to glycyrrhizin is inhibited in this strain. All 4 offspring of the 83-555 strain produced glucoglycyrrhizin. Interestingly, 2 of the offspring produced both glycyrrhizin and glucoglycyrrhizin, and sequence analysis of the pkr gene suggested that these 2 offspring were hybrids of 83-555 strain and glycyrrhizin-producing strains.
authors.affiliation: School of Pharmacy, Iwate Medical University.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Hayashi
authors.forename: Isao
authors.initials: I
authors.lastname: Fujii
authors.forename: Munekazu
authors.initials: M
authors.lastname: Iinuma
authors.forename: Makio
authors.initials: M
authors.lastname: Shibano
authors.forename: Kazuo
authors.initials: K
authors.lastname: Ozaki
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Watanabe
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis; D017343:Genes, Plant / Q000235:genetics; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification*; D029882:Glycyrrhiza uralensis / Q000737:chemistry* / Q000235:genetics; D019695:Glycyrrhizic Acid; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000737:chemistry; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D018744:DNA, Plant; D019695:Glycyrrhizic Acid; D000429:Alcohol Oxidoreductases; C087192:polyketide synthase ketoreductase; D006034:Glycyrrhetinic Acid