title: Flavobacterium limnosediminis sp. nov., isolated from sediment of a freshwater lake.
abstract: A Gram-stain-negative, rod-shaped, pale yellow, aerobic bacterial strain, JC2902(T), was isolated from a sediment sample of Ungok Lake in Gochang, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain JC2902(T) belongs to the genus Flavobacterium and forms a distinct phyletic line within a clade containing four recognized species of the genus Flavobacterium. The genomic relatedness between strain JC2902(T) and closely related strains was calculated using average nucleotide identity values of whole genome sequences, which indicated that the new isolate represents a novel genomic species. Through comparison of chemotaxonomic and other phenotypic characteristics between strain JC2902(T) and the type strains of the four phylogenetically related species, a number of characteristics differentiated strain JC2902(T) from the previously described type strains. Differential characteristics of strain JC2902(T) include fatty acid profiles, cellular motility, inability to grow on Luria-Bertani and tripticase soy agar media, and absence of N-acetyl-β-glucosaminidase and flexirubin-type pigments. Based on data from this polyphasic taxonomic study, strain JC2902(T) is considered to represent a novel species of the genus Flavobacterium, for which the name Flavobacterium limnosediminis sp. nov. is proposed. The type strain is JC2902(T) ( = KACC 16937(T) = JCM 18661(T)).
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Kihyun
authors.initials: K
authors.lastname: Lee
authors.affiliation: Interdisciplinary Program in Bioinformatics, Seoul National University, Seoul, Republic of Korea.
authors.forename: Sang-Cheol
authors.initials: SC
authors.lastname: Park
authors.affiliation: Korea University Guro Hospital, Korea University College of Medicine, Seoul, Republic of Korea.|Department of Public Health Science, Graduate School, Korea University, Seoul, Republic of Korea.
authors.forename: Hana
authors.initials: H
authors.lastname: Yi
authors.affiliation: Interdisciplinary Program in Bioinformatics, Seoul National University, Seoul, Republic of Korea.|School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jongsik
authors.initials: J
authors.lastname: Chun
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology*; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011090:Polyenes / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011090:Polyenes; D012336:RNA, Ribosomal, 16S; C017313:flexirubins