title: Orthogonal test design for optimization of lipid accumulation and lipid property in Nannochloropsis oculata for biodiesel production.
abstract: In order to improve the property of Nannochloropsis oculata as biodiesel feedstock, a L9(3(4)) orthogonal test on limited nitrogen supplementation (0, 0.22 and 0.44 mmol N L(-1)), high iron concentration (1.2×10(-2), 1.2×10(-1) and 1.2 mmol Fe L(-1)) and culture temperature (10, 20 and 30 °C) was conducted to select the most effective combinational measurement. Results showed that microalgae displayed the highest total lipid content (60.44±0.68%), the highest neutral lipid proportion (90.74±0.18%), the highest lipid yield (152.70±7.40 mg L(-1)) and the largest cetane number (CN, 64.34±0.13) under different combined conditions. There were significant interaction among nitrogen supplementation, iron concentration and culture temperature on the lipid yield and CN of N. oculata. Nitrogen supplementation, followed by iron concentration and temperature orderly, was the most influential factor in lipid yield. It is therefore suggested that the combination of 0.44 mmol N L(-1), 1.2×10(-1) mmol Fe L(-1) and 20 °C was the best measurement for improving the property of N. oculata as biodiesel feedstock.
authors.affiliation: Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture, No. 999, Hucheng Ring Road, Lingang New City, Shanghai 201306, People's Republic of China.
authors.forename: Likun
authors.initials: L
authors.lastname: Wei
authors.affiliation: Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture, No. 999, Hucheng Ring Road, Lingang New City, Shanghai 201306, People's Republic of China; Shanghai Engineering Research Center of Aquaculture, No. 999, Hucheng Ring Road, Lingang New City, Shanghai 201306, People's Republic of China; Shanghai University Knowledge Service Platform, Shanghai Ocean University Aquatic Animal Breeding Center (ZF1206), No. 999, Hucheng Ring Road, Lingang New City, Shanghai 201306, People's Republic of China. Electronic address: xxhuang@shou.edu.cn.
authors.forename: Xuxiong
authors.initials: X
authors.lastname: Huang
authors.affiliation: Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture, No. 999, Hucheng Ring Road, Lingang New City, Shanghai 201306, People's Republic of China.
authors.forename: Zhenzheng
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture, No. 999, Hucheng Ring Road, Lingang New City, Shanghai 201306, People's Republic of China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D056804:Biofuels; D008401:Gas Chromatography-Mass Spectrometry; D007501:Iron / Q000378:metabolism; D050356:Lipid Metabolism; D009584:Nitrogen / Q000378:metabolism; D058009:Stramenopiles / Q000378:metabolism*
chemical_list: D056804:Biofuels; D007501:Iron; D009584:Nitrogen
keywords: Biodiesel; Iron concentration; Nannochloropsis oculata; Nitrogen supplementation; Orthogonal test