title: Comparative genome analysis of Mycobacterium avium revealed genetic diversity in strains that cause pulmonary and disseminated disease.
abstract: Mycobacterium avium complex (MAC) infection causes disseminated disease in immunocompromised hosts, such as human immunodeficiency virus (HIV)-positive patients, and pulmonary disease in persons without systemic immunosuppression, which has been increasing in many countries. In Japan, the incidence of pulmonary MAC disease caused by M. avium is about 7 times higher than that caused by M. intracellulare. To explore the bacterial factors that affect the pathological state of MAC disease caused by M. avium, we determined the complete genome sequence of the previously unreported M. avium subsp. hominissuis strain TH135 isolated from a HIV-negative patient with pulmonary MAC disease and compared it with the known genomic sequence of M. avium strain 104 derived from an acquired immunodeficiency syndrome patient with MAC disease. The genome of strain TH135 consists of a 4,951,217-bp circular chromosome with 4,636 coding sequences. Comparative analysis revealed that 4,012 genes are shared between the two strains, and strains TH135 and 104 have 624 and 1,108 unique genes, respectively. Many strain-specific regions including virulence-associated genes were found in genomes of both strains, and except for some regions, the G+C content in the specific regions was low compared with the mean G+C content of the corresponding chromosome. Screening of clinical isolates for genes located in the strain-specific regions revealed that the detection rates of strain TH135-specific genes were relatively high in specimens isolated from pulmonary MAC disease patients, while, those of strain 104-specific genes were relatively high in those from HIV-positive patients. Collectively, M. avium strains that cause pulmonary and disseminated disease possess genetically distinct features, and it suggests that the acquisition of specific genes during strain evolution has played an important role in the pathological manifestations of MAC disease.
authors.affiliation: Department of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, Japan.
authors.forename: Kei-ichi
authors.initials: K
authors.lastname: Uchiya
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Takahashi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yagi
authors.forename: Makoto
authors.initials: M
authors.lastname: Moriyama
authors.forename: Takayuki
authors.initials: T
authors.lastname: Inagaki
authors.forename: Kazuya
authors.initials: K
authors.lastname: Ichikawa
authors.forename: Taku
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Nikai
authors.forename: Kenji
authors.initials: K
authors.lastname: Ogawa
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics; D001482:Base Composition / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004270:DNA, Circular / Q000737:chemistry / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D014644:Genetic Variation; D016680:Genome, Bacterial / Q000235:genetics; D023281:Genomics / Q000379:methods*; D006801:Humans; D008171:Lung Diseases / Q000150:complications / Q000382:microbiology*; D008969:Molecular Sequence Data; D015269:Mycobacterium avium Complex / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D015270:Mycobacterium avium-intracellulare Infection / Q000150:complications / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004270:DNA, Circular