title: Phosphorylation of Calcineurin at a novel serine-proline rich region orchestrates hyphal growth and virulence in Aspergillus fumigatus.
abstract: The fungus Aspergillus fumigatus is a leading infectious killer in immunocompromised patients. Calcineurin, a calmodulin (CaM)-dependent protein phosphatase comprised of calcineurin A (CnaA) and calcineurin B (CnaB) subunits, localizes at the hyphal tips and septa to direct A. fumigatus invasion and virulence. Here we identified a novel serine-proline rich region (SPRR) located between two conserved CnaA domains, the CnaB-binding helix and the CaM-binding domain, that is evolutionarily conserved and unique to filamentous fungi and also completely absent in human calcineurin. Phosphopeptide enrichment and tandem mass spectrometry revealed the phosphorylation of A. fumigatus CnaA in vivo at four clustered serine residues (S406, S408, S410 and S413) in the SPRR. Mutation of the SPRR serine residues to block phosphorylation led to significant hyphal growth and virulence defects, indicating the requirement of calcineurin phosphorylation at the SPRR for its activity and function. Complementation analyses of the A. fumigatus ΔcnaA strain with cnaA homologs from the pathogenic basidiomycete Cryptococcus neoformans, the pathogenic zygomycete Mucor circinelloides, the closely related filamentous fungi Neurospora crassa, and the plant pathogen Magnaporthe grisea, revealed filamentous fungal-specific phosphorylation of CnaA in the SPRR and SPRR homology-dependent restoration of hyphal growth. Surprisingly, circular dichroism studies revealed that, despite proximity to the CaM-binding domain of CnaA, phosphorylation of the SPRR does not alter protein folding following CaM binding. Furthermore, mutational analyses in the catalytic domain, CnaB-binding helix, and the CaM-binding domains revealed that while the conserved PxIxIT substrate binding motif in CnaA is indispensable for septal localization, CaM is required for its function at the hyphal septum but not for septal localization. We defined an evolutionarily conserved novel mode of calcineurin regulation by phosphorylation in filamentous fungi in a region absent in humans. These findings suggest the possibility of harnessing this unique SPRR for innovative antifungal drug design to combat invasive aspergillosis.
authors.affiliation: Department of Pediatrics, Division of Pediatric Infectious Diseases, Duke University Medical Center, Durham, North Carolina, United States of America.
authors.forename: Praveen R
authors.initials: PR
authors.lastname: Juvvadi
authors.forename: Christopher
authors.initials: C
authors.lastname: Gehrke
authors.forename: Jarrod R
authors.initials: JR
authors.lastname: Fortwendel
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lamoth
authors.forename: Erik J
authors.initials: EJ
authors.lastname: Soderblom
authors.forename: Erik C
authors.initials: EC
authors.lastname: Cook
authors.forename: Michael A
authors.initials: MA
authors.lastname: Hast
authors.forename: Yohannes G
authors.initials: YG
authors.lastname: Asfaw
authors.forename: M Arthur
authors.initials: MA
authors.lastname: Moseley
authors.forename: Trevor P
authors.initials: TP
authors.lastname: Creamer
authors.forename: William J
authors.initials: WJ
authors.lastname: Steinbach
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000627:therapeutic use; D001228:Aspergillosis / Q000188:drug therapy / Q000201:enzymology / Q000235:genetics; D001232:Aspergillus fumigatus / Q000201:enzymology* / Q000235:genetics; D019703:Calcineurin / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D065095:Calcineurin Inhibitors; D005656:Fungal Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D025301:Hyphae / Q000201:enzymology* / Q000235:genetics; D008297:Male; D051379:Mice; D008954:Models, Biological; D010766:Phosphorylation; D017434:Protein Structure, Tertiary
chemical_list: D000935:Antifungal Agents; D065095:Calcineurin Inhibitors; D005656:Fungal Proteins; D019703:Calcineurin