title: Antioxidant activity of a novel synthetic hexa-peptide derived from an enzymatic hydrolysate of duck skin by-products.
abstract: A peptide was synthesized on the basis of our previous study from solid phase peptide synthesis using ASP48S (Peptron Inc.) and identified by the reverse phase high-performance liquid chromatography (HPLC) using a Vydac Everest C18 column. The molecular mass of the peptide found to be 693.90 Da, and the amino acid sequences of the peptide was Trp-Tyr-Pro-Ala-Ala-Pro. The purpose of this study was to evaluate antioxidant effects of the peptide by electron spin resonance (ESR) spectrometer, and on t-BHP-induced liver cells damage in Chang cells. The antioxidative activity of the peptide was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, alkyl and superoxide radical scavenging activity using an ESR spectrometer. The half maximal inhibitory concentration (IC50) value of the peptide for hydroxyl, DPPH, alkyl, and superoxide radical scavenging activity were 45.2, 18.5, 31.5, and 33.4 μM, respectively. In addition, the peptide inhibited productions of cell death against t-BHP-induced liver cell damage in Chang cells. It was presumed to be peptide involved in regulating the apoptosis-related gene expression in the cell environment. The present results indicate that the peptide substantially contributes to antioxidative properties in liver cells.
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju 380-701, Republic of Korea.
authors.forename: Seung-Jae
authors.initials: SJ
authors.lastname: Lee
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Cheong
authors.forename: Yon-Suk
authors.initials: YS
authors.lastname: Kim
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Hwang
authors.forename: Hyuck-Ju
authors.initials: HJ
authors.lastname: Kwon
authors.forename: Seo-Hee
authors.initials: SH
authors.lastname: Kang
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Moon
authors.forename: Byong-Tae
authors.initials: BT
authors.lastname: Jeon
authors.forename: Pyo-Jam
authors.initials: PJ
authors.lastname: Park
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004372:Ducks; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D008099:Liver / Q000166:cytology / Q000187:drug effects; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology; D011492:Protein Hydrolysates; D012867:Skin / Q000737:chemistry*; D013481:Superoxides / Q000378:metabolism; D020122:tert-Butylhydroperoxide / Q000633:toxicity
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010455:Peptides; D020011:Protective Agents; D011492:Protein Hydrolysates; D013481:Superoxides; D020122:tert-Butylhydroperoxide
keywords: Antioxidant activity; Electron spin resonance spectrometer; Free radicals; High-performance liquid chromatography; Synthetic hexa-peptide