title: microRNA-9 targets the long non-coding RNA MALAT1 for degradation in the nucleus.
abstract: microRNAs regulate the expression of over 60% of protein coding genes by targeting their mRNAs to AGO2-containing complexes in the cytoplasm and promoting their translational inhibition and/or degradation. There is little evidence so far for microRNA-mediated regulation of other classes of non-coding RNAs. Here we report that microRNA-9 (miR-9) regulates the expression of the Metastasis Associated Lung Adenocarcinoma Transcript 1 (MALAT-1), one of the most abundant and conserved long non-coding RNAs. Intriguingly, we find that miR-9 targets AGO2-mediated regulation of MALAT1 in the nucleus. Our findings reveal a novel direct regulatory link between two important classes of non-coding RNAs, miRs and lncRNAs, and advance our understanding of microRNA functions.
authors.affiliation: 1] Biotech Research and Innovation Centre and Centre for Epigenetics, University of Copenhagen, Denmark [2].
authors.forename: Eleonora
authors.initials: E
authors.lastname: Leucci
authors.forename: Francesca
authors.initials: F
authors.lastname: Patella
authors.forename: Johannes
authors.initials: J
authors.lastname: Waage
authors.forename: Kim
authors.initials: K
authors.lastname: Holmstrøm
authors.forename: Morten
authors.initials: M
authors.lastname: Lindow
authors.forename: Bo
authors.initials: B
authors.lastname: Porse
authors.forename: Sakari
authors.initials: S
authors.lastname: Kauppinen
authors.forename: Anders H
authors.initials: AH
authors.lastname: Lund
mesh_terms: D060565:Argonaute Proteins / Q000378:metabolism; D020029:Base Pairing; D001483:Base Sequence; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009690:Nucleic Acid Conformation; D020871:RNA Stability; D062085:RNA, Long Noncoding / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: C466422:AGO2 protein, human; D060565:Argonaute Proteins; C557041:MALAT1 long non-coding RNA, human; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding