title: Anti-inflammatory effects of total isoflavones from Pueraria lobata on cerebral ischemia in rats.
abstract: Puerariae radix, the dried root of Pueraria lobata Ohwi, is one of earliest and most important edible crude herbs used for various medical purposes in Oriental medicine. The aim of the present study was to determine the anti-inflammatory effects of Total Isoflavones from P. lobata (TIPL), which contains the unique isoflavone puerarin, in ischemia in vivo models. Oral administration of TIPL (100 mg/kg) reduced the brain infarct volume and attenuated ischemia-induced cyclooxygenase-2 (COX-2) up-regulation at 2 days after middle cerebral artery occlusion (MCAo) in rats. Moreover, TIPL reduced activation of glial fibrillary acid protein (GFAP) and CD11b antibody (OX-42) at 7 days after MCAo in hippocampal CA1 region. These results show that TIPL can protect the brain from ischemic damage after MCAo. Regarding the immunohistochemical study, the effects of TIPL may be attributable to its anti-inflammatory properties by the inhibition of COX-2 expression, astrocyte expression, and microglia.
authors.affiliation: Functionality Evaluation Research Group, Korea Food Research Institute, Seongnam 463-746, Korea.
authors.forename: Dong Wook
authors.initials: DW
authors.lastname: Lim
authors.forename: Changho
authors.initials: C
authors.lastname: Lee
authors.forename: In-Ho
authors.initials: IH
authors.lastname: Kim
authors.forename: Yun Tai
authors.initials: YT
authors.lastname: Kim
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001253:Astrocytes / Q000378:metabolism; D056547:CA1 Region, Hippocampal / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D039481:CD11b Antigen / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D020244:Infarction, Middle Cerebral Artery / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D007529:Isoflavones / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D029908:Pueraria / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000893:Anti-Inflammatory Agents; D039481:CD11b Antigen; D005904:Glial Fibrillary Acidic Protein; D007529:Isoflavones; D010936:Plant Extracts; D051546:Cyclooxygenase 2