title: Distinct reactivity of the commercially available monoclonal antibodies of D-dimer and plasma FDP testing to the molecular variants of fibrin degradation products.
abstract: Fibrin degradation products (FDP) are an important marker of coagulopathy. We assessed the reactivity of the monoclonal antibodies used in clinical laboratory testing (6 D-dimer reagents, D-dimer-1-6; 4 plasma FDP reagents, plasma FDP-1-4) to quantify FDP using in vitro-generated FDP as well as FDP in clinical samples. The monoclonal antibodies used in D-dimer-1, -2, -5, and -6 reacted poorly to the low molecular weight forms of in vitro-generated FDP. The monoclonal antibodies used in D-dimer-3 and -4 had better reactivity to the low molecular weight forms of in vitro-generated FDP. The monoclonal antibodies used in plasma FDP-2, -3, and -4 reacted well to the high and low molecular weight FDP forms, while the monoclonal antibody in plasma FDP-1 reacted poorly to the low molecular weight FDP forms. Analysis of clinical samples revealed deviations in FDP molecular weight forms in DIC samples. The reactivity of the monoclonal antibodies of laboratory FDP testing to FDP variants in clinical samples was similar to that of in vitro-generated FDP. In conclusion, the monoclonal antibodies used in clinical laboratories to detect FDP have distinct reactivity to the molecular variants of FDP generated in vitro as well as those present in clinical samples. Our findings support the consensus for the standardization of D-dimer and plasma FDP testing.
authors.affiliation: Division of Cell and Molecular Medicine, Center for Molecular Medicine, Hematology Division of Department of Internal Medicine, Jichi Medical University and Jichi Medical University Hospital, Shimotsuke, Tochigi-ken 329-0498, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Madoiwa
authors.forename: Isao
authors.initials: I
authors.lastname: Kitajima
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Ohmori
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Sakata
authors.forename: Jun
authors.initials: J
authors.lastname: Mimuro
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D004211:Disseminated Intravascular Coagulation / Q000097:blood*; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D054556:Venous Thromboembolism / Q000097:blood*
chemical_list: D000911:Antibodies, Monoclonal; D005338:Fibrin Fibrinogen Degradation Products; C036309:fibrin fragment D
keywords: D-dimer; DIC; FDP; FgDP; VTE; disseminated intravascular coagulation; fibrin degradation product; fibrinogen degradation product; monoclonal antibody; venous thromboembolism