title: Real-time PCR for differentiation of F18 variants among enterotoxigenic and Shiga toxin-producing Escherichia coli from piglets with diarrhoea and oedema disease.
abstract: One-step real-time PCR using one set of primers and four probes was developed for differentiation of F18 variants (F18 common, F18ab, F18ac, F18new variant) of enterotoxigenic (ETEC) and Shiga toxin-producing (STEC) Escherichia coli from piglets with diarrhoea and oedema disease. The limits of detection for F18common, F18ab, F18ac, and F18new variant were 10(7), 10(7), 10(5) and 10(7)colony forming units/g faeces, respectively. Of 94 Korean isolates of E. coli encoding F18, 70 were F18ac (43 STEC/ETEC, 4 STEC and 23 ETEC), 15 were F18ab (all STEC) and nine were F18new variant (1 STEC/ETEC, 7 STEC, 1 ETEC).
authors.affiliation: Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, 175 Anyangro Manangu, Anyang 430-757, Republic of Korea; College of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea. Electronic address: jaewon8911@korea.kr.
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Byun
authors.forename: Byeong Yeal
authors.initials: BY
authors.lastname: Jung
authors.forename: Ha-Young
authors.initials: HY
authors.lastname: Kim
authors.forename: John M
authors.initials: JM
authors.lastname: Fairbrother
authors.forename: Myoung-Heon
authors.initials: MH
authors.lastname: Lee
authors.forename: Wan-Kyu
authors.initials: WK
authors.lastname: Lee
mesh_terms: D000818:Animals; D003967:Diarrhea / Q000175:diagnosis / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*; D004488:Edema Disease of Swine / Q000175:diagnosis* / Q000235:genetics / Q000382:microbiology; D054307:Enterotoxigenic Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D036781:Fimbriae Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods* / Q000662:veterinary; D056910:Republic of Korea; D017422:Sequence Analysis, DNA / Q000662:veterinary; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000235:genetics / Q000382:microbiology
chemical_list: D029968:Escherichia coli Proteins; C113053:FedA protein, E coli; D036781:Fimbriae Proteins
keywords: Enteropathogenic Escherichia coli; F18 variants; Pigs; Real-time PCR