title: Antioxidant and cholinesterase inhibitory activity of a new peptide from Ziziphus jujuba fruits.
abstract: Antioxidant agents and cholinesterase inhibitors are the foremost drugs for the treatment of Alzheimer's disease (AD). In this study, a new peptide from Ziziphus jujuba fruits was investigated for its inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes as well as antioxidant activity. This peptide was introduced as a new peptide and named Snakin-Z. The Snakin-Z displayed considerable cholinesterase inhibition against AChE and BChE. The half maximal inhibitory concentration (IC50) values of Snakin-Z against AChE and BChE are 0.58 ± 0.08 and 0.72 ± 0.085 mg/mL, respectively. This peptide has 80% enzyme inhibitory activity on AChE and BChE at 1.5 mg/mL. The Snakin-Z also had the high antioxidant activity (IC50 = 0.75 ± 0.09 mg/mL). Thus, it is suggested that Snakin-Z may be beneficial in the treatment of AD. However, more detailed researches are still required as in vivo testing its anticholinesterase and antioxidant activities.
authors.affiliation: 1Young Researchers and Elite Club, Yazd Branch, Islamic Azad University, Yazd, Islamic Republic of Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Zare-Zardini
authors.forename: Behnaz
authors.initials: B
authors.lastname: Tolueinia
authors.forename: Azam
authors.initials: A
authors.lastname: Hashemi
authors.forename: Leila
authors.initials: L
authors.lastname: Ebrahimi
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Fesahat
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy / Q000201:enzymology*; D000595:Amino Acid Sequence; D000975:Antioxidants / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D005638:Fruit; D008969:Molecular Sequence Data; D010455:Peptides / Q000494:pharmacology*; D028321:Plant Preparations / Q000494:pharmacology*; D031957:Ziziphus / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D010455:Peptides; D028321:Plant Preparations
keywords: Alzheimer’s disease; Ziziphus jujuba fruits; acetylcholinesterase; antioxidant; butyrylcholinesterase