title: Chemically induced pluripotent stem cells (CiPSCs): a transgene-free approach.
abstract: Induced pluripotent stem cells (iPSCs) could be generated by a single gene Oct4 and chemical compounds, in which exogenous expression of Oct4 was indispensable for reprogramming. Recent advances in chemical-mediated cellular reprogramming suggest that small molecules alone (i.e. without Yamanaka factors) can successfully establish iPSCs from mouse somatic cells.
authors.affiliation: Gene Expression Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Masuda
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Hishida
authors.forename: Ganesh N
authors.initials: GN
authors.lastname: Pandian
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Izpisua Belmonte
mesh_terms: D000818:Animals; D065150:Cellular Reprogramming / Q000187:drug effects; D005576:Colforsin / Q000494:pharmacology*; D004622:Embryo, Mammalian / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D019076:Transgenes / Q000235:genetics*
chemical_list: D050814:Octamer Transcription Factor-3; D049452:Green Fluorescent Proteins; D005576:Colforsin