title: A myeloid-derived suppressor cell-mediated T-cell suppression assay for functional evaluation of immune cells in tumor-bearing mice.
abstract: This protocol describes a procedure to evaluate the ability of Gr-1(+) CD11b(+) myeloid-derived suppressor cells (MDSCs) isolated from the spleen or tumor of a tumor-bearing mouse to suppress antigen-specific T-cell proliferation. MDSCs are cultured with responder splenocytes from an OT-1 T-cell receptor transgenic mouse stimulated with cognate peptide (OVA-257 peptide) or with an irrelevant, control peptide. This protocol spans a 4-d period. The readout is T-cell proliferation by (3)[H]-thymidine incorporation during an 18-h pulse between Days 3 and 4.
authors.affiliation: Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.
authors.forename: Lauren J
authors.initials: LJ
authors.lastname: Bayne
authors.forename: Robert H
authors.initials: RH
authors.lastname: Vonderheide
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D007165:Immunosuppression Therapy / Q000379:methods*; D051379:Mice; D008822:Mice, Transgenic; D009369:Neoplasms / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D010047:Ovalbumin