title: [Influence of HLA-E polymorphism on cytomegalovirus infection after HLA-matched hematopoietic stem cell transplantation].
abstract: This study was aimed to explore the potential association of HLA-E polymorphism with the incidence of cytomegalovirus (CMV) infection after HLA-matched hematopoietic stem cell transplantation, 119 HLA-genoidentical sibling pairs for HLA-E polymorphism were analyzed, HLA-E DNA was amplified by polymerase chain reaction (PCR), and the amplified DNA products was also sequenced directly after purification to confirm the genotype. The results showed that the homozygous HLA-E*0101/0101 accounted for 20.17%, the homozygous HLA-E*0103/0103 accounted for 27.73%; heterozygous HLA-E*0101/0103 accounted for 52.10%; in homozygous HLA-E*0101/0101 group, 15 cases were infected with CMV and the CMV infection rate was 62.50%; in homozygous HLA-E*0103/0103 group, 16 cases were infected with CMV and the CMV infection rate was 48.48%; in heterozygous HLA-E*0101/0103 group 20 cases were infected with CMV and the CMV infection rate was 32.25%. As compared with the homozygous HLA-E*0101/0101 group, the CMV infection rate in HLA-E*0103 group displays statistical significance (P = 0.0295). The CMV infection rate occurred higher and its significance is statistical (P = 0.0074). It is concluded that the HLA-E gene polymorphism is associated with CMV infection after HLA-genoidentical bone marrow transplantation.
authors.affiliation: Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, Jiangsu Province, China.
authors.forename: Zi-Ling
authors.initials: ZL
authors.lastname: Zhu
authors.forename: Xiao-Jin
authors.initials: XJ
authors.lastname: Wu
authors.forename: De-Pei
authors.initials: DP
authors.lastname: Wu
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Feng
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Xiao
authors.initials: X
authors.lastname: Ma
authors.forename: Yue
authors.initials: Y
authors.lastname: Han
authors.forename: Yue-Jun
authors.initials: YJ
authors.lastname: Liu
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D003586:Cytomegalovirus Infections / Q000209:etiology / Q000235:genetics*; D005260:Female; D005838:Genotype; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D035781:Siblings; D055815:Young Adult; D000097625:HLA-E Antigens
chemical_list: D015395:Histocompatibility Antigens Class I; D000097625:HLA-E Antigens