title: Mitochondrial targeting functional peptides as potential devices for the mitochondrial delivery of a DF-MITO-Porter.
abstract: To achieve mitochondrial therapy, we previously reported on the use of an octaarginine (R8) modified Dual Function (DF)-MITO-Porter for delivering molecules to mitochondria in living cells. In this study, using isolated mitochondria, homogenates and living cells, we evaluated the utility of mitochondrial targeting functional peptides as a ligand for delivering carriers. The S2 peptide modified carrier showed a high mitochondrial targeting activity in homogenates and living cells. In addition, the S2 peptide had a lower cell toxicity compared to R8 modified liposomes. The S2 peptide represents a potentially useful moiety for constructing an efficient and safe mitochondrial delivery system.
authors.affiliation: Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Kawamura
authors.forename: Yuma
authors.initials: Y
authors.lastname: Yamada
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Harashima
mesh_terms: D000818:Animals; D004337:Drug Carriers; D006367:HeLa Cells; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D051381:Rats
chemical_list: D004337:Drug Carriers; D010455:Peptides
keywords: MITO-Porter; Mitochondria; Mitochondrial drug delivery; Mitochondrial targeting peptide; Nanoparticles; S2 peptide