title: Identification of circulating porcine-human reassortant G4P[6] rotavirus from children with acute diarrhea in China by whole genome analyses.
abstract: P[6] group A rotavirus (RVA) strains identified in four stool specimens collected from children with acute diarrhea in Guangxi Province, southern China in 2010, with unknown G type were further analyzed by full genomic analysis. It was revealed by whole genome sequencing that 11 genomic cognate gene segments of these P[6] RVA strains shared almost 100% nucleotide identities and all exhibited an identical G4-P[6]-I1-R1-C1-M1-A8-N1-T1-E1-H1 genotype constellation. Phylogenetic analyses of VP7, VP1-VP4, NSP1, NSP2, NSP4 and NSP5 genes revealed that these Guangxi G4P[6] RVA strains were closely related to porcine and porcine-like human RVAs, while VP6 and NSP3 were closely related to those of common human RVAs. Interestingly, the four infants from whom these specimens were collected had come from different villages and/or towns. They had not contacted with each other and had had acute diarrhea before admitted into the same hospital. The genomic analyses and the clinical data revealed that these four Guangxi G4P[6] RVA strains from China were reassortants possessing VP6 and NSP3 gene segments of human origin yet all other nine gene segments of porcine origin. It is the first report on porcine-human reassortant G4P[6] RVA with identical genome configuration circulating in children.
authors.affiliation: Laboratory of Virology, Capital Institute of Pediatrics, Beijing 100020, China.
authors.forename: Hui-Jin
authors.initials: HJ
authors.lastname: Dong
authors.forename: Yuan
authors.initials: Y
authors.lastname: Qian
authors.forename: Teng
authors.initials: T
authors.lastname: Huang
authors.forename: Ru-Nan
authors.initials: RN
authors.lastname: Zhu
authors.forename: Lin-Qing
authors.initials: LQ
authors.lastname: Zhao
authors.forename: You
authors.initials: Y
authors.lastname: Zhang
authors.forename: Rong-Cheng
authors.initials: RC
authors.lastname: Li
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology*; D005243:Feces / Q000821:virology; D016679:Genome, Viral / Q000235:genetics; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D010802:Phylogeny; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000235:genetics* / Q000821:virology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D014764:Viral Proteins
keywords: Child diarrhea; Full genome analysis; G4P[6]; Porcine–human; Reassortant; Rotavirus