title: [Effect of indole-3-carbinol and rutin on rats' provision by vitamins' A and E with different fat content in its diet].
abstract: Effect of indole-3-carbinol (I-3-C) and rutin (R) supplementation on vitamins A and E status of growing Wistar rats, receiving for 6 or 4 week semi-synthetic diets with different levels (1, 11 and 31%) of fat (lard and sunflower oil at a ratio of 1:1) has been studied. The content of vitamin E was 6, 9 and 15 IU, vitamin A - 400 IU in 100 g of ration. Against the various fat content during the last 7 or 14 days of the experiment rats received respectively I-3-C (20 mg per 1 kg of body weight per day) or R (0.4% of the feed weight). Rat tissues were analyzed for vitamins A (retinol and retinyol palmitate) and E (alpha-tocopherol) by HPLC. Reducing fat content in diet from 11 to 1% was associated with significant (p<0.05) decrease of hepatic retinyl palmitate and alpha-tocopherol (1,6-1,7 times) with constant plasma concentration of retinol and alpha-tocopherol. Raising fat content from 11 to 31% , in contrast, led to increased levels of hepatic retinyl palmitate and alpha-tocopherol respectively by 13% (p=0.248) and 89% (p=0.006) and plasma ROL of 26% (p=0,024), while the plasma concentration of alpha- tocopherol has not changed. I-3-C and R do not affect the availability of vitamin E in rats, regardless of the fat content in the diet. With excess fat content (31%) in the diet, supplementation of I-3-C and R lowered hepatic RP by 22-52% (p<0.05) compared to rats receiving a diet with adequate fat. Adding of I-3-C to the high-fat diets resulted to a significant reduction of vitamin A concentration in blood plasma by 12% (p=0.024) and in liver by 37% (p=0.002).
authors.forename: N A
authors.initials: NA
authors.lastname: Beketova
authors.forename: L V
authors.initials: LV
authors.lastname: Kravchenko
authors.forename: O V
authors.initials: OV
authors.lastname: Kosheleva
authors.forename: O A
authors.initials: OA
authors.lastname: Vrzhesinskaia
authors.forename: V M
authors.initials: VM
authors.lastname: Kodentsova
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D004041:Dietary Fats / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012431:Rutin / Q000494:pharmacology*; D014801:Vitamin A / Q000097:blood*; D014810:Vitamin E / Q000097:blood*
chemical_list: D016588:Anticarcinogenic Agents; D004041:Dietary Fats; D007211:Indoles; D014801:Vitamin A; D014810:Vitamin E; D012431:Rutin; C016517:indole-3-carbinol