title: Chemical fragmentation for massively parallel sequencing library preparation.
abstract: Fragmentation is essential in most library preparation protocols for use with massively parallel sequencing systems. Complexes that generate hydroxyl radicals, such as iron-EDTA, can be used to introduce random DNA cleavage. Here we describe a chemical fragmentation method that can be incorporated into library preparation protocols for next-generation sequencing workflows. This protocol has been validated by whole genome, amplicon and exome sequencing. Chemical fragmentation is a cost-effective alternative to current fragmentation methods that has no observable sequence bias and requires no instrumentation.
authors.affiliation: KTH Royal Institute of Technology, Science for Life Laboratory, School of Biotechnology, Division of Gene Technology, Solna SE-171 65, Sweden. Electronic address: gyarmp@gmail.com.
authors.forename: P
authors.initials: P
authors.lastname: Gyarmati
authors.forename: Y
authors.initials: Y
authors.lastname: Song
authors.forename: J
authors.initials: J
authors.lastname: Hällman
authors.forename: M
authors.initials: M
authors.lastname: Käller
mesh_terms: D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*
keywords: Fragmentation; High-throughput sequencing; Library preparation