title: Chemical transformation of yeast.
abstract: Transformation of chemically competent yeast cells is a method for introducing exogenous DNA into living cells. Typically, the DNA is either a plasmid carrying an autonomous replication sequence that allows for propagation or a linear piece of DNA to be integrated into the genome. The DNA usually also carries a marker that allows for selection of successfully transformed cells by plating on the appropriate selective media.
authors.affiliation: Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA.
authors.forename: Megan
authors.initials: M
authors.lastname: Bergkessel
authors.forename: Christine
authors.initials: C
authors.lastname: Guthrie
mesh_terms: D004271:DNA, Fungal / Q000737:chemistry* / Q000235:genetics; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal
keywords: Autonomous replication sequence; Competent cells preparation; Polymerase chain reaction (PCR); Transformation of yeast cells; YPAD medium