title: Vitamin C in infant formula and adult/pediatric nutritional formula by HPLC with UV detection: First Action 2012.21.
abstract: This method for the determination of vitamin C by HPLC allows for the separation and quantitation of L-ascorbic acid in infant, pediatric, and adult nutritional products. Liquids, semisolids, and powders ranging from 2 to 1000 mg/kg in their consumable forms were analyzed during the method validation. The method met the standard method performance requirements and was approved by an AOAC Expert Review Panel on Infant Formula and Adult Nutritionals on October 2, 2012. During validation, the overall intermediate precision was 2.1% RSD (triplicate determinations on 7-10 days); the within-day precision, or repeatability, was 1.54% RSD (triplicate determinations). Accuracy, as spike recovery, ranged from 97.0 to 100.9%. The method detection and quantitation limits were determined experimentally to be 0.02 and 0.06 mg/L, respectively, in prepared samples.
authors.affiliation: Abbott Nutrition, 3300 Stelzer Rd, Nutritional Research Center, Columbus, OH 43219, USA. karen.schimpf@abbott.com
authors.forename: Karen
authors.initials: K
authors.lastname: Schimpf
authors.forename: Linda
authors.initials: L
authors.lastname: Thompson
authors.forename: Steve
authors.initials: S
authors.lastname: Baugh
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D041943:Infant Formula / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001205:Ascorbic Acid