title: [Thyroid hormone inhibits the growth of pancreatic cancer xenograft in nude mice].
abstract: OBJECTIVE
  To investigate the therapeutic effect of thyroid hormone in nude mice bearing human pancreatic cancer xenograft.
  
  
  METHODS
  A BALB/c nude mouse model bearing pancreatic cancer was established with human pancreatic cancer cell line Bx-PC3. The mouse models were divided randomly into 5 groups, namely the control group treated with distilled water, high and low concentrations of thyroid hormone (T3) groups, and high and low concentration of propylthiouracil (PTU) groups. After intervention for 21 days, the changes in body weight and xenograft tumor volume and weight were measured, and the serum T3 concentration was detected by ELISA assay. The expression of proliferating cell nuclear antigen (PCNA) and microvessel density (MVD) were detected using immunohistochemistry.
  
  
  RESULTS
  The body weight of nude mice in T3 groups was significantly reduced after intervention, while that in PTU groups showed no obvious changes. Compared with PTU groups and control group, T3 groups showed significantly reduced tumor volume and weight (P<0.05) with also reduced PCNA expression and MVD, but these effect did not exhibit a dose dependence (P>0.05).
  
  
  CONCLUSIONS
  Thyroid hormone can inhibit the growth of human pancreatic cancer in nude mice by suppressing the proliferation and angiogenesis of the tumor cells, suggesting the potential value of thyroid hormone in pancreatic cancer therapy.
authors.affiliation: Department of Gastroenterology Diseases, Second Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi'an 710004, China. E-mail: pumpkingua@126.com.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Boxin
authors.initials: B
authors.lastname: Shang
authors.forename: Haitao
authors.initials: H
authors.lastname: Shi
authors.forename: Hanwei
authors.initials: H
authors.lastname: Ma
authors.forename: Jiong
authors.initials: J
authors.lastname: Jiang
authors.forename: Bin
authors.initials: B
authors.lastname: Qin
authors.forename: Lei
authors.initials: L
authors.lastname: Dong
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D055806:Microvessels / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000517:prevention & control*; D010190:Pancreatic Neoplasms / Q000098:blood supply* / Q000473:pathology; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D014284:Triiodothyronine / Q000494:pharmacology*; D023041:Xenograft Model Antitumor Assays
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D014284:Triiodothyronine