title: SUMO-specific protease 2 suppresses cell migration and invasion through inhibiting the expression of MMP13 in bladder cancer cells.
abstract: BACKGROUND
  SUMO-specific protease 2 (SENP2) is a de-SUMOylation protease family member which has an indispensable role in the regulation of NF-κB transcriptional activation and Wnt signaling. However, whether SENP2 plays a role in tumor metastasis is completely unknown.
  
  
  METHODS
  Real-time PCR and Western blot was used to detect the expression of SENP2 in human bladder cancer samples and cell lines. Small interfering RNA (siRNA) was used to silencing the expression of SENP2. Matrigel-coated invasion chambers were used to detect the invasion ability of SENP2 in bladder cancer cells.
  
  
  RESULTS
  SENP2 was down-regulated in bladder cancer samples. SENP2 inhibited bladder cancer cells migration and invasion in vitro. Transcriptional analysis of several genes associated with tumor metastasis and invasion demonstrated that SENP2 selectively down-regulated MMP13 in bladder cancer cells. Further analysis indicated that silencing of MMP13 rescued the invasive phenotype in SENP2 expressing T24 cells.
  
  
  CONCLUSIONS
  SENP2 functions as a tumor metastasis suppressor in bladder cancer. The effects of SENP2 on bladder cancer invasion are partially mediated by inhibiting the expression of MMP13.
authors.affiliation: Department of Urology, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai, China.
authors.forename: Ming-Yue
authors.initials: MY
authors.lastname: Tan
authors.forename: Xing-Yu
authors.initials: XY
authors.lastname: Mu
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Er-Dun
authors.initials: ED
authors.lastname: Bao
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Qiu
authors.forename: Yu
authors.initials: Y
authors.lastname: Fan
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D057809:HEK293 Cells; D006801:Humans; D053509:Matrix Metalloproteinase 13 / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000473:pathology
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D003546:Cysteine Endopeptidases; C501833:SENP2 protein, human; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13