title: Immunohistochemical analysis of TIMP-3 and MMP-9 in actinic keratosis, squamous cell carcinoma of the skin, and basal cell carcinoma.
abstract: The expression of metalloproteinases and their inhibitors has been related to different invasive and metastatic potentials in cancer. This study aims to investigate the immunohistochemical expression of TIMP-3 and MMP-9 in samples of basal cell carcinoma (BCC), squamous cell carcinoma of the skin (SCC), and actinic keratosis (AK). Immunohistochemistry was performed to evaluate the expression of TIMP-3 and MMP-9 in samples of BCC (n=22), SCC (n=10), and AK (n=15). Ten fields of both tumor parenchyma and tumor stroma were photographed and counted in image software. The ratio of positive cells to total cells was used to quantify the staining. A higher expression of MMP-9 was found in tumor stroma of SCC compared to BCC and AK. No significant differences in TIMP-3 expression were observed among the groups. Considering the well-described differences between these neoplasms, these results provide additional evidence of the role of MMP-9 in tumor invasiveness of keratinocyte-derived tumors.
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Minas Gerais, Brazil; Department of Medicine, Universidade Estadual de Montes Claros, Minas Gerais, Brazil.
authors.forename: Fabiano O
authors.initials: FO
authors.lastname: Poswar
authors.forename: Carlos A C
authors.initials: CA
authors.lastname: Fraga
authors.forename: Lucyana C
authors.initials: LC
authors.lastname: Farias
authors.forename: John D
authors.initials: JD
authors.lastname: Feltenberger
authors.forename: Vitória P D
authors.initials: VP
authors.lastname: Cruz
authors.forename: Sérgio H S
authors.initials: SH
authors.lastname: Santos
authors.forename: Christine M
authors.initials: CM
authors.lastname: Silveira
authors.forename: Alfredo M B
authors.initials: AM
authors.lastname: de Paula
authors.forename: André L S
authors.initials: AL
authors.lastname: Guimarães
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D002280:Basal Cell Carcinoma / Q000201:enzymology* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D055623:Keratosis, Actinic / Q000201:enzymology* / Q000473:pathology; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000032:analysis*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D012878:Skin Neoplasms / Q000201:enzymology* / Q000473:pathology; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; D019717:Tissue Inhibitor of Metalloproteinase-3; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: Actinic keratosis; Basal cell carcinoma; Matrix metalloproteinase-9; Squamous cell carcinoma; Tissue inhibitor of metalloproteinase 3