title: Multiple gene genealogical analyses of a nematophagous fungus Paecilomyces lilacinus from China.
abstract: Paecilomyces lilacinus is a geographically widespread nematophagous fungus and a promising biological control agent against plant parasitic nematodes. However, relatively little is known about its patterns of genetic variation through its broad geographic and ecological contexts. In this study, we analyzed the genetic variation of 2 virulence-associated genes (PLS and PLC) and 4 housekeeping gene fragments (ITS, RPB1, RPB2, and β-tubulin) among 80 P. lilacinus specimens collected from 7 locations in China. Various degrees of polymorphism and haplotype diversity were observed among the six gene fragments. However, no genetic differentiation was observed among the geographic populations, consistent with extensive gene flow among these geographic populations of P. lilacinus in China. Our analysis also suggested that clonal reproduction was the predominant mode of reproduction in natural populations of P. lilacinus.
authors.affiliation: Laboratory for Conservation and Utilization of Bio-resources, and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, Yunnan 650091, P. R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Bi
authors.forename: Ke-Qin
authors.initials: KQ
authors.lastname: Zhang
mesh_terms: D004783:Environmental Microbiology; D019143:Evolution, Molecular; D005800:Genes, Fungal; D008040:Genetic Linkage; D014644:Genetic Variation; D005828:Genetics, Population; D006239:Haplotypes; D015810:Linkage Disequilibrium; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D009154:Mutation; D016366:Open Reading Frames; D010143:Paecilomyces / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic