title: Calcium imaging of vomeronasal organ response using slice preparations from transgenic mice expressing G-CaMP2.
abstract: The vomeronasal organ (VNO) in vertebrate animals detects pheromones and interspecies chemical signals. We describe in this chapter a Ca(2+) imaging approach using transgenic mice that express the genetically encoded Ca(2+) sensor G-CaMP2 in VNO tissue. This approach allows us to analyze the complex patterns of the vomeronasal neuron response to large number of chemosensory stimuli.
authors.affiliation: Stowers Institute for Medical Research, Kansas City, MO, USA.
authors.forename: C Ron
authors.initials: CR
authors.lastname: Yu
mesh_terms: D000818:Animals; D002118:Calcium / Q000737:chemistry; D002147:Calmodulin / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D052139:Intracellular Calcium-Sensing Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009219:Myosin-Light-Chain Kinase / Q000235:genetics; D009831:Olfactory Mucosa / Q000294:innervation / Q000378:metabolism; D010446:Peptide Fragments / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D019147:Vomeronasal Organ / Q000294:innervation* / Q000378:metabolism*
chemical_list: D002147:Calmodulin; D052139:Intracellular Calcium-Sensing Proteins; C107660:M13 protein (myosin light-chain kinase); D010446:Peptide Fragments; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D009219:Myosin-Light-Chain Kinase; D002118:Calcium