title: Pesticide residue screening using a novel artificial neural network combined with a bioelectric cellular biosensor.
abstract: We developed a novel artificial neural network (ANN) system able to detect and classify pesticide residues. The novel ANN is coupled, in a customized way, to a cellular biosensor operation based on the bioelectric recognition assay (BERA) and able to simultaneously assay eight samples in three minutes. The novel system was developed using the data (time series) of the electrophysiological responses of three different cultured cell lines against three different pesticide groups (carbamates, pyrethroids, and organophosphates). Using the novel system, we were able to classify correctly the presence of the investigated pesticide groups with an overall success rate of 83.6%. Considering that only 70,000-80,000 samples are annually tested in Europe with current conventional technologies (an extremely minor fraction of the actual screening needs), the system reported in the present study could contribute to a screening system milestone for the future landscape in food safety control.
authors.affiliation: Laboratory of Informatics, School of Food Science, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.
authors.forename: Konstantinos P
authors.initials: KP
authors.lastname: Ferentinos
authors.forename: Costas P
authors.initials: CP
authors.lastname: Yialouris
authors.forename: Petros
authors.initials: P
authors.lastname: Blouchos
authors.forename: Georgia
authors.initials: G
authors.lastname: Moschopoulou
authors.forename: Spyridon
authors.initials: S
authors.lastname: Kintzios
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002219:Carbamates / Q000032:analysis; D002460:Cell Line; D004560:Electricity; D006801:Humans; D051379:Mice; D016571:Neural Networks, Computer; D010755:Organophosphates / Q000032:analysis; D010573:Pesticide Residues / Q000032:analysis*; D011722:Pyrethrins / Q000032:analysis
chemical_list: D002219:Carbamates; D010755:Organophosphates; D010573:Pesticide Residues; D011722:Pyrethrins