title: 4-Phenyl-α-cyanocinnamic acid amide: screening for a negative ion matrix for MALDI-MS imaging of multiple lipid classes.
abstract: Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) has become a method of choice in lipid analysis, as it provides localization information for defined lipids that is not readily accessible with nonmass spectrometric methods. Most current MALDI matrices have been found empirically. Nevertheless, preferential matrix properties for many analyte classes are poorly understood and may differ between lipid classes. We used rational matrix design and semiautomated screening for the discovery of new matrices suitable for MALDI-IMS of lipids. Utilizing Smartbeam- and nitrogen lasers for MALDI, we systematically compared doubly substituted α-cyanocinnamic acid derivatives (R(1)-CCA-R(2)) with respect to their ability to serve as negative ion matrix for various brain lipids. We identified 4-phenyl-α-cyanocinnamic acid amide (Ph-CCA-NH2) as a novel negative ion matrix that enables analysis and imaging of various lipid classes by MALDI-MS. We demonstrate that Ph-CCA-NH2 displays superior sensitivity and reproducibility compared to matrices commonly employed for lipids. A relatively small number of background peaks and good matrix suppression effect could make Ph-CCA-NH2 a widely applicable tool for lipid analysis.
authors.affiliation: Applied Research Center in Biomedical Mass Spectrometry (ABIMAS), ‡Instrumental Analysis and Bioanalysis, Mannheim University of Applied Sciences , Paul-Wittsack-Strasse 10, 68163 Mannheim, Germany.
authors.forename: Annabelle
authors.initials: A
authors.lastname: Fülöp
authors.forename: Martina B
authors.initials: MB
authors.lastname: Porada
authors.forename: Christian
authors.initials: C
authors.lastname: Marsching
authors.forename: Henning
authors.initials: H
authors.lastname: Blott
authors.forename: Björn
authors.initials: B
authors.lastname: Meyer
authors.forename: Suparna
authors.initials: S
authors.lastname: Tambe
authors.forename: Roger
authors.initials: R
authors.lastname: Sandhoff
authors.forename: Hans-Dieter
authors.initials: HD
authors.lastname: Junker
authors.forename: Carsten
authors.initials: C
authors.lastname: Hopf
mesh_terms: D000818:Animals; D001921:Brain; D002934:Cinnamates / Q000138:chemical synthesis / Q000737:chemistry*; D008055:Lipids / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: C587811:4-phenyl-alpha-cyanocinnamic acid amide; D002934:Cinnamates; D008055:Lipids