title: Progressive changes in inflammatory and matrix adherence of bronchial epithelial cells with persistent respiratory syncytial virus (RSV) infection (progressive changes in RSV infection).
abstract: In addition to the acute manifestations of respiratory syncytial virus (RSV), persistent infection may be associated with long-term complications in the development of chronic respiratory diseases. To understand the mechanisms underlying RSV-induced long-term consequences, we established an in vitro RSV (strain A2) infection model using human bronchial epithelial (16HBE) cells that persists over four generations and analyzed cell inflammation and matrix adherence. Cells infected with RSV at multiplicity of infection (MOI) 0.0067 experienced cytolytic or abortive infections in the second generation (G2) or G3 but mostly survived up to G4. Cell morphology, leukocyte and matrix adherence of the cells did not change in G1 or G2, but subsequently, leukocyte adherence and cytokine/chemokine secretion, partially mediated by intercellular adhesion molecule-1 (ICAM-1), increased drastically, and matrix adherence, partially mediated by E-cadherin, decreased until the cells died. Tumor necrosis factor-α (TNF-α) secretion was inhibited by ICAM-1 antibody in infected-16HBE cells, suggesting that positive feedback between TNF-α secretion and ICAM-1 expression may be significant in exacerbated inflammation. These data demonstrate the susceptibility of 16HBE cells to RSV and their capacity to produce long-term progressive RSV infection, which may contribute to inflammation mobilization and epithelial shedding.
authors.affiliation: Department of Physiology, School of Basic Medical Science, Central South University, Changsha 410078, China. alicelxa@gmail.com
authors.forename: Xiaoai
authors.initials: X
authors.lastname: Liu
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Qin
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiang
authors.forename: Huijun
authors.initials: H
authors.lastname: Liu
authors.forename: Ge
authors.initials: G
authors.lastname: Gao
authors.forename: Ling
authors.initials: L
authors.lastname: Qin
authors.forename: Chi
authors.initials: C
authors.lastname: Liu
authors.forename: Xiangping
authors.initials: X
authors.lastname: Qu
mesh_terms: D015153:Blotting, Western; D002448:Cell Adhesion / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000821:virology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology* / Q000821:virology; D012136:Respiratory Syncytial Viruses / Q000472:pathogenicity; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1