title: Circadian measurements of sirtuin biology.
abstract: Many of our behavioral and physiological processes display daily oscillations that are under the control of the circadian clock. The core molecular clock network is present in both the brain and peripheral tissues and is composed of a complex series of interlocking transcriptional/translational feedback loops that oscillate with a periodicity of ~24 h. Recent evidence has implicated NAD(+) biosynthesis and the sirtuin family of NAD(+)-dependent protein deacetylases as part of a novel feedback loop within the core clock network, findings which underscore the importance of taking circadian timing into consideration when designing and interpreting metabolic studies, particularly in regard to sirtuin biology. Thus, this chapter introduces both in vivo and in vitro circadian methods to analyze various sirtuin-related endpoints across the light-dark cycle and discusses the transcriptional, biochemical, and physiological outputs of the clock.
authors.affiliation: Division of Endocrinology, Metabolism and Molecular Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
authors.forename: Kathryn Moynihan
authors.initials: KM
authors.lastname: Ramsey
authors.forename: Alison H
authors.initials: AH
authors.lastname: Affinati
authors.forename: Clara B
authors.initials: CB
authors.lastname: Peek
authors.forename: Biliana
authors.initials: B
authors.lastname: Marcheva
authors.forename: Hee-Kyung
authors.initials: HK
authors.lastname: Hong
authors.forename: Joseph
authors.initials: J
authors.lastname: Bass
mesh_terms: D000818:Animals; D057906:Circadian Clocks / Q000502:physiology*; D002940:Circadian Rhythm / Q000502:physiology*; D025461:Feedback, Physiological; D008124:Locomotion; D051379:Mice; D008810:Mice, Inbred C57BL; D009243:NAD / Q000378:metabolism*; D041681:NIH 3T3 Cells; D037761:Sirtuins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D009243:NAD; D037761:Sirtuins