title: Ligation Chain Reaction based gold nanoparticle assembly for ultrasensitive DNA detection.
abstract: A simple and ultrasensitive DNA biosensor was developed utilizing the color and size changes originating from Ligation Chain Reaction (LCR)-based gold nanoparticle assembly as an indicator. Using synthetic DNA of 10-fold serial dilutions as a target, the limit of detection (LOD) was 1.5 aM with a range of DNA concentrations from 0.01 to 1000 fM by UV-vis absorption and 0.1 aM in the range of 0.01-10,000 fM by dynamic light scattering (DLS). This developed method is low-cost, rapid, and much lower LOD for the detection of specific short DNA sequences, and the results can be directly determined either by the color or by the DLS. The capability of high-throughput detection with the aid of PCR amplification apparatus can also be realized.
authors.affiliation: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, China; Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, China.
authors.forename: Honghong
authors.initials: H
authors.lastname: Yin
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.forename: Liguang
authors.initials: L
authors.lastname: Xu
authors.forename: Shuifang
authors.initials: S
authors.lastname: Zhu
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000302:isolation & purification*; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*
chemical_list: D006046:Gold; D004247:DNA
keywords: Assembly; DNA detection; Gold nanoparticle; Ligation Chain Reaction; Ultrasensitive