title: Femtosecond digital lensless holographic microscopy to image biological samples.
abstract: The use of femtosecond laser radiation in digital lensless holographic microscopy (DLHM) to image biological samples is presented. A mode-locked Ti:Sa laser that emits ultrashort pulses of 12 fs intensity FWHM, with 800 nm mean wavelength, at 75 MHz repetition rate is used as a light source. For comparison purposes, the light from a light-emitting diode is also used. A section of the head of a drosophila melanogaster fly is studied with both light sources. The experimental results show very different effects of the pinhole size on the spatial resolution with DLHM. Unaware phenomena on the field of the DLHM are analyzed.
authors.affiliation: Institut de Noves Tecnologies de la Imatge (INIT), Universitat Jaume I, 12071 Castelló, Spain.
authors.forename: Omel
authors.initials: O
authors.lastname: Mendoza-Yero
authors.forename: Alejandro
authors.initials: A
authors.lastname: Calabuig
authors.forename: Enrique
authors.initials: E
authors.lastname: Tajahuerce
authors.forename: Jesús
authors.initials: J
authors.lastname: Lancis
authors.forename: Pedro
authors.initials: P
authors.lastname: Andrés
authors.forename: Jorge
authors.initials: J
authors.lastname: Garcia-Sucerquia
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster; D006257:Head; D006696:Holography / Q000295:instrumentation / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors; D017971:Tin Compounds
chemical_list: D017971:Tin Compounds; C109984:indium tin oxide