title: Molecular evolution of porcine reproductive and respiratory syndrome virus isolates from central China.
abstract: To investigate the genetic diversity of prevailing porcine reproductive and respiratory syndrome virus (PRRSV) in Henan Province of China, 61 ORF5 gene sequences, originating from Henan Province during 2003-2010, were subjected to amino acid variation and phylogenetic analysis. The analyzed PRRSV ORF5 sequences carried evidence of one unique recombination event. Phylogenetic analysis revealed that all Henan isolates belonged to type 2 genotype and were divided into two subgroups. The dominant isolates had shifted from subgroup 1 to subgroup 2 during 2003-2010. Amino acid variation analysis of the glycoprotein 5 revealed that Henan PRRSV strains tended to accumulate more substitutions within the N-terminus and hypervariable region. Selective pressure analysis revealed evidence that some ORF5 sites have likely evolved in response to immune pressure.
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Chunlong
authors.initials: C
authors.lastname: Mu
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Lu
authors.forename: Erzhen
authors.initials: E
authors.lastname: Duan
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Weijuan
authors.initials: W
authors.lastname: Li
authors.forename: Fenghua
authors.initials: F
authors.lastname: Zhang
authors.forename: Darren P
authors.initials: DP
authors.lastname: Martin
authors.forename: Mingfan
authors.initials: M
authors.lastname: Yang
authors.forename: Pingan
authors.initials: P
authors.lastname: Xia
authors.forename: Baoan
authors.initials: B
authors.lastname: Cui
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D019318:Porcine Reproductive and Respiratory Syndrome / Q000453:epidemiology / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics*; D016415:Sequence Alignment / Q000662:veterinary; D013552:Swine; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins; C114789:glycoprotein 5, PRRSV
keywords: Genetic variation; ORF5; PRRSV; Phylogenetic analysis