title: Acetylcholinesterase based biosensor for monitoring of Malathion and Acephate in food samples: a voltammetric study.
abstract: Acetylcholinesterase (AChE) biosensor was developed through silica sol-gel (SiSG) immobilisation of AChE on the carbon paste electrode (CPE) and used as working electrode. AChE catalyses the cleavage of acetylthiocholine chloride (ASChCl or substrate) to thiocholine, which was oxidised to give a disulphide compound by dimerisation at 0.60V versus saturated calomel electrode. All the experiments were carried out in 0.1M phosphate buffer solution (PBS) at pH 7.0 and 0.1M KCl solution at room temperature. The limit of detection and limit of quantification values were found to be 0.058ppm, 0.044ppm and 0.194ppm, 0.147ppm for Malathion and Acephate, respectively. The response of the biosensor showed a good linearity range with an incubation time of 4min for Malathion and Acephate, respectively. This biosensor was used for the direct determination of pesticides without any pretreatment and it requires less time for analysis.
authors.affiliation: Electrochemical Research Laboratory, Department of Chemistry, S.V.U. College of Sciences, Sri Venkateswara University, Tirupati 517502 Andhra Pradesh, India.
authors.forename: P
authors.initials: P
authors.lastname: Raghu
authors.forename: T
authors.initials: T
authors.lastname: Madhusudana Reddy
authors.forename: K
authors.initials: K
authors.lastname: Reddaiah
authors.forename: B E
authors.initials: BE
authors.lastname: Kumara Swamy
authors.forename: M
authors.initials: M
authors.lastname: Sreedhar
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002523:Edible Grain / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005506:Food Contamination / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D008294:Malathion / Q000032:analysis*; D009946:Organothiophosphorus Compounds / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D063088:Phosphoramides
chemical_list: D004800:Enzymes, Immobilized; D009946:Organothiophosphorus Compounds; D010575:Pesticides; D063088:Phosphoramides; C001969:acephate; D000110:Acetylcholinesterase; D008294:Malathion
keywords: Acetylthiocholine chloride; Biosensor; Carbon paste electrode; Immobilisation; Malathion and Acephate