title: Detection of Salmonella typhimurium in retail chicken meat and chicken giblets.
abstract: OBJECTIVE
  To detect Salmonella typhimurium (S. typhimurium), one of the most frequently isolated serovars from food borne outbreaks throughout the world, in retail raw chicken meat and giblets.
  
  
  METHODS
  One hundred samples of retail raw chicken meat and giblets (Liver, heart and gizzard) which were collected from Assiut city markets for detection of the organism and by using Duplex PCR ampliﬁcation of DNA using rfbJ and fliC genes.
  
  
  RESULTS
  S. typhimurium was detected at rate of 44%, 40% and 48% in chicken meat, liver and heart, respectively, but not detected in gizzard.
  
  
  CONCLUSIONS
  The results showed high incidence of S. typhimurium in the examined samples and greater emphasis should be applied on prevention and control of contamination during processing for reducing food-borne risks to consumers.
authors.affiliation: Department of Food Hygiene, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt. doaassiut@yahoo.com
authors.forename: Doaa M Abd
authors.initials: DM
authors.lastname: El-Aziz
mesh_terms: D000818:Animals; D002645:Chickens / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005895:Gizzard, Avian / Q000382:microbiology; D006321:Heart / Q000382:microbiology; D015994:Incidence; D008099:Liver / Q000382:microbiology; D008460:Meat / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012486:Salmonella typhimurium / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial
keywords: Chicken meat; Duplex PCR; Salmonella typhimurium