title: Oral administration of benzyl-isothiocyanate inhibits in vivo growth of subcutaneous xenograft tumors of human malignant melanoma A375.S2 cells.
abstract: A number of experiments have demonstrated that benzyl-isothiocyanate (BITC) induces cytotoxic cell death through the induction of apoptosis in various human cancer cell lines. In the present study, we investigated the effects of BITC on the growth of A375.S2 cell xenograft tumors in nude BALB/c mice in vivo. The A375.S2 cancer cells were inoculated subcutaneously into the lower flanks of each nude mouse. After cancer cell inoculation, all animals were maintained in the animal room for seven days and all mice produced one palpable tumor. Animals were randomly divided into two groups, each mouse was individually given intraperitoneal injections of BITC (20 mg/kg) or not (control). Results from the in vivo experiments indicated that BITC did not significantly affect the body weight of nude BALB/c mice bearing xenograft A375.S2 cell tumors but did significantly decrease the tumor weight.
authors.affiliation: Department of Food and Beverage Management, NanKai University of Technology, Nan-Tou County, Taiwan, ROC.
authors.forename: Wei-Ya
authors.initials: WY
authors.lastname: Ni
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Hsiao
authors.forename: Shu-Chun
authors.initials: SC
authors.lastname: Hsu
authors.forename: Shu-Ching
authors.initials: SC
authors.lastname: Hsueh
authors.forename: Chuan-Hsun
authors.initials: CH
authors.lastname: Chang
authors.forename: Bin-Chuan
authors.initials: BC
authors.lastname: Ji
authors.forename: Jai-Sing
authors.initials: JS
authors.lastname: Yang
authors.forename: Hsu-Feng
authors.initials: HF
authors.lastname: Lu
authors.forename: Jing-Gung
authors.initials: JG
authors.lastname: Chung
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D006801:Humans; D017879:Isothiocyanates / Q000008:administration & dosage*; D008297:Male; D008545:Melanoma / Q000188:drug therapy / Q000473:pathology*; D051379:Mice; D047368:Tumor Burden / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D017879:Isothiocyanates; C031403:benzyl isothiocyanate
keywords: A375.S2 melanoma cells; BITC; in vivo; nude mice