title: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) induction on Snail expression during mouse decidualization.
abstract: Embryo implantation requires a precise synchronism between the receptive uterus and activated blastocyst and is regulated by complicated molecular networks. Although many implantation-related genes have been identified, the crosstalk among them is still unknown. Snail, a transcription repressor, plays a central role during epithelial-mesenchymal transition. Our previous study showed that Snail is highly expressed at implantation site in mouse uterus. This study was to examine how Snail is related with other implantation-related genes in mice. Uterine stromal cells were isolated from mouse uteri on day 4 of pregnancy and treated with HB-EGF. Snail was induced significantly by HB-EGF. By using specific inhibitors and siRNA, we demonstrated that HB-EGF induction on Snail expression is dependent on the EGFR-ERK-Stat3 pathway. Cox-2 was regulated by Snail. The current findings demonstrate that Snail can relate with HB-EGF, Stat3 and Cox-2 and may play a role during mouse embryo implantation and decidualization.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; School of Life Science, Anhui Medical University, Hefei, China; College of Life Science, Xiamen University, Xiamen 361005, China.
authors.forename: Xiu-Hong
authors.initials: XH
authors.lastname: Zhang
authors.forename: Xuan
authors.initials: X
authors.lastname: Liang
authors.forename: Tong-Song
authors.initials: TS
authors.lastname: Wang
authors.forename: Xiao-Huan
authors.initials: XH
authors.lastname: Liang
authors.forename: Ru-Juan
authors.initials: RJ
authors.lastname: Zuo
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Deng
authors.forename: Zhi-Rong
authors.initials: ZR
authors.lastname: Zhang
authors.forename: Fu-Niu
authors.initials: FN
authors.lastname: Qin
authors.forename: Zhen-Ao
authors.initials: ZA
authors.lastname: Zhao
authors.forename: Zeng-Ming
authors.initials: ZM
authors.lastname: Yang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D003656:Decidua / Q000378:metabolism*; D010064:Embryo Implantation; D066246:ErbB Receptors / Q000378:metabolism; D005260:Female; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins / Q000502:physiology*; D020935:MAP Kinase Signaling System; D008297:Male; D051379:Mice; D010766:Phosphorylation; D011247:Pregnancy; D011499:Protein Processing, Post-Translational; D050796:STAT3 Transcription Factor / Q000378:metabolism; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: C587884:Hbegf protein, mouse; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D050796:STAT3 Transcription Factor; D000071250:Snail Family Transcription Factors; C494087:Stat3 protein, mouse; D014157:Transcription Factors; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; C541485:EGFR protein, mouse; D066246:ErbB Receptors
keywords: Decidua; Embryo implantation; HB-EGF; Snail; Uterus