title: Crystallization and preliminary X-ray diffraction analysis of human importin β-Snail zinc finger domain complex.
abstract: Snail is a C2H2-type zinc finger transcriptional repressor that induces epithelial-mesenchymal transition by repression of E-cadherin expression levels during embryonic development and tumour progression. Snail is imported into the nucleus by importin β through direct binding with its four zinc finger domain. The complex between importin β and Snail four zinc finger domain was crystallized in order to understand the nuclear transport mechanism of Snail. The constituents of the complex were separately expressed and were then co-purified and crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to space group C2, with unit-cell parameters a = 228.2, b = 77.5, c = 72.0 Å, β = 100.9° and diffracted to 2.5 Å resolution.
authors.affiliation: College of Pharmacy, Chungbuk National University, 410 Seungbong, Heungduk, Cheongju 361-763, Republic of Korea.
authors.forename: Saehae
authors.initials: S
authors.lastname: Choi
authors.forename: Jinsue
authors.initials: J
authors.lastname: Song
authors.forename: Se-Young
authors.initials: SY
authors.lastname: Son
authors.forename: Il Yeong
authors.initials: IY
authors.lastname: Park
authors.forename: Eiki
authors.initials: E
authors.lastname: Yamashita
authors.forename: Soo Jae
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D021581:Active Transport, Cell Nucleus; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D028961:beta Karyopherins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D011993:Recombinant Fusion Proteins; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D028961:beta Karyopherins
keywords: Snail; importin β; zinc finger domain