title: Assays for GH, IGF-I, and IGF binding protein-3.
abstract: The growth hormone/insulin-like growth factor (GH/IGF) axis is widely investigated in disorders of growth, development, and anterior pituitary function. Methods are described for three hormones in this axis (growth hormone, IGF-I, and IGF binding protein-3). Enzyme-linked immunosorbent assays (ELISAs) are described as they combine the specificity of antibody-antigen interactions with the sensitivity of simple enzyme assays. Many commercial immunoassays are available for the measurement of GH and IGF-I, and the main problems encountered in the measurement of these clinically important hormones are discussed.
authors.affiliation: Department of Clinical Biochemistry, John Radcliffe Hospital, Oxford, UK.
authors.forename: Nishan
authors.initials: N
authors.lastname: Guha
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D019382:Human Growth Hormone / Q000097:blood*; D006801:Humans; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000097:blood*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D013048:Specimen Handling / Q000379:methods
chemical_list: D018972:Insulin-Like Growth Factor Binding Protein 3; D019382:Human Growth Hormone; D007334:Insulin-Like Growth Factor I