title: Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis.
abstract: Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique.
authors.affiliation: Nuffield Department of Obstetrics and Gynaecology, University of Oxford, Level 3, Women's Centre, John Radcliffe Hospital, Oxford, UK.
authors.forename: Chris
authors.initials: C
authors.lastname: Gardiner
authors.forename: Yannick J
authors.initials: YJ
authors.lastname: Ferreira
authors.forename: Rebecca A
authors.initials: RA
authors.lastname: Dragovic
authors.forename: Christopher W G
authors.initials: CW
authors.lastname: Redman
authors.forename: Ian L
authors.initials: IL
authors.lastname: Sargent
keywords: extracellular vesicles; light scattering; nanoparticle tracking analysis; standardisation