title: Upregulated expression of NF-YC contributes to neuronal apoptosis via proapoptotic protein bim in rats' brain hippocampus following middle cerebral artery occlusion (MCAO).
abstract: Cerebral ischemia represents a severe brain injury that could lead to significant neuronal damage and death. In this study, we performed a middle cerebral artery occlusion (MCAO) in adult rats and observed that a subunit of nuclear factor-Y (NF-Y) transcriptional factor, NF-YC, was accumulated in rat hippocampal CA1 neurons. Immunochemistrical and immunofluorescent analysis revealed that NF-YC was primarily expressed in the nucleus of neurons. Meanwhile, we found that the changes of bim, one of the target genes of NF-Y, were consistent with the expression of NF-YC and Bim was mainly located in the NF-YC positive cells. Moreover, there was a concomitant upregulation of active caspase-3 and TUNEL positive cells. Taken together, these results suggested that the upregulation of NF-YC might play an important role in the pathophysiology via proapoptotic protein Bim after MCAO and further research is needed to have a better understanding of its function and mechanism.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, 215006, People's Republic of China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Wang
authors.forename: Chunhua
authors.initials: C
authors.lastname: Wan
authors.forename: Shanshan
authors.initials: S
authors.lastname: Yu
authors.forename: Lixiang
authors.initials: L
authors.lastname: Yang
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Ting
authors.initials: T
authors.lastname: Lu
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Bi
authors.forename: Junkang
authors.initials: J
authors.lastname: Jiang
authors.forename: Gang
authors.initials: G
authors.lastname: Cui
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics* / Q000502:physiology; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D000072224:Bcl-2-Like Protein 11; D056547:CA1 Region, Hippocampal / Q000473:pathology* / Q000502:physiology; D023081:CCAAT-Binding Factor / Q000235:genetics* / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D004195:Disease Models, Animal; D020287:In Situ Nick-End Labeling; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D009474:Neurons / Q000473:pathology* / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000502:physiology
chemical_list: D051017:Apoptosis Regulatory Proteins; D000072224:Bcl-2-Like Protein 11; C000606778:Bcl2l11 protein, rat; D023081:CCAAT-Binding Factor; D008565:Membrane Proteins; C000593008:Nfyc protein, rat; D011518:Proto-Oncogene Proteins; C402244:nuclear factor Y; C505410:Casp3 protein, rat; D053148:Caspase 3