title: Synthesis and effects of conjugated tocopherol analogues on peptide nucleic acid hybridisation.
abstract: To the N-terminus of a nonamer peptide nucleic acid sequence, H-GCACGACTT-NH2, was attached a number of lipophilic conjugate molecules including three synthetic tocopherol (vitamin E) analogues. Studies were then undertaken with complementary PNA and DNA sequences to explore the effects of the conjugates using the techniques of UV monitored melting curves and isothermal calorimetry. Duplex formation was observed when the benzopyran group of vitamin E was conjugated. However, in the presence of the phytyl chain of vitamin E, binding was found to be temperature dependent.
authors.affiliation: Department of Chemistry, La Trobe University, Bundoora, Australia. b.abbott@latrobe.edu.au.
authors.forename: Elisse C
authors.initials: EC
authors.lastname: Browne
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Langford
authors.forename: Belinda M
authors.initials: BM
authors.lastname: Abbott
mesh_terms: D001665:Binding Sites; D015195:Drug Design; D015394:Molecular Structure; D009693:Nucleic Acid Hybridization; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature; D024505:Tocopherols / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D020135:Peptide Nucleic Acids; D024505:Tocopherols