title: Biosurfactant production by Serratia rubidaea SNAU02 isolated from hydrocarbon contaminated soil and its physico-chemical characterization.
abstract: The aim of the study was to characterize and optimize the growth media for biosurfactant production from Serratia rubidaea SNAU02 isolated from hydrocarbon-contaminated soil from Cuddalore district, Tamilnadu, India. The biosurfactant produced by S. rubidaea SNAU02, was able to reduce the surface tension to 34.4 mN m(-1) in MSM medium. The biosurfactant was characterized by FT-IR and GC-MS analysis. The GC-MS analysis shows that dirhamnolipid was detected in abundance as predominant congener than monorhamnolipid. The response surface methodology (RSM) -central composite design (CCD) was performed to optimize the media for biosurfactant production. The maximum emulsification index was obtained under the optimal condition of 29.31 g L(-1) mannitol; 2.06 g L(-1) yeast extract, medium pH 6.97 and 5.69 g L(-1) NaCl. The biosurfactant produced by S. rubidaea recovered 92% of used engine oil adsorbed to a sand sample, suggested the potential application in microbial enhanced oil recovery and bioremediation.
authors.affiliation: Department of Microbiology, Faculty of Science, Annamalai University, Annamalai Nagar 608002, Tamil Nadu, India. Electronic address: snalini.msc@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Nalini
authors.affiliation: Department of Microbiology, Faculty of Agriculture, Annamalai University, Annamalai Nagar 608002, Tamil Nadu, India.
authors.forename: R
authors.initials: R
authors.lastname: Parthasarathi
mesh_terms: D006838:Hydrocarbons / Q000378:metabolism*; D012705:Serratia / Q000378:metabolism*; D012989:Soil Pollutants / Q000378:metabolism*; D013501:Surface-Active Agents / Q000378:metabolism*
chemical_list: D006838:Hydrocarbons; D012989:Soil Pollutants; D013501:Surface-Active Agents
keywords: Emulsification index; GC–MS; Optimization; Rhamnolipid; Serratia rubidaea