title: Immunization of Mastomys coucha with Brugia malayi recombinant trehalose-6-phosphate phosphatase results in significant protection against homologous challenge infection.
abstract: Development of a vaccine to prevent or reduce parasite development in lymphatic filariasis would be a complementary approach to existing chemotherapeutic tools. Trehalose-6-phosphate phosphatase of Brugia malayi (Bm-TPP) represents an attractive vaccine target due to its absence in mammals, prevalence in the major life stages of the parasite and immunoreactivity with human bancroftian antibodies, especially from endemic normal subjects. We have recently reported on the cloning, expression, purification and biochemical characterization of this vital enzyme of B. malayi. In the present study, immunoprophylactic evaluation of Bm-TPP was carried out against B. malayi larval challenge in a susceptible host Mastomys coucha and the protective ability of the recombinant protein was evaluated by observing the adverse effects on microfilarial density and adult worm establishment. Immunization caused 78.4% decrease in microfilaremia and 71.04% reduction in the adult worm establishment along with sterilization of 70.06% of the recovered live females. The recombinant protein elicited a mixed Th1/Th2 type of protective immune response as evidenced by the generation of both pro- and anti-inflammatory cytokines IL-2, IFN-γ, TNF-α, IL-4 and an increased production of antibody isotypes IgG1, IgG2a, IgG2b and IgA. Thus immunization with Bm-TPP conferred considerable protection against B. malayi establishment by engendering a long-lasting effective immune response and therefore emerges as a potential vaccine candidate against lymphatic filariasis (LF).
authors.affiliation: Division of Parasitology, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India.
authors.forename: Susheela
authors.initials: S
authors.lastname: Kushwaha
authors.forename: Prashant Kumar
authors.initials: PK
authors.lastname: Singh
authors.forename: Ajay Kumar
authors.initials: AK
authors.lastname: Rana
authors.forename: Shailja
authors.initials: S
authors.lastname: Misra-Bhattacharya
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D017178:Brugia malayi / Q000201:enzymology / Q000235:genetics / Q000276:immunology; D005368:Filariasis / Q000201:enzymology / Q000235:genetics / Q000276:immunology / Q000517:prevention & control; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D051189:Murinae / Q000276:immunology* / Q000469:parasitology; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D015801:Helminth Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011994:Recombinant Proteins; C023178:trehalose-phosphatase; D010744:Phosphoric Monoester Hydrolases