title: [The value of low-molecular-weight DNA of blood plasma in the diagnostic of the patological processes of different genesis].
abstract: The low-molecular-weight DNA appears in blood plasma of irradiated rats, and its content correlates directly with the irradiation dose. Cloning has shown, that enrichment of low-molecular-weight DNA with G+C content and features of its nucleotide sequences point to its ability to form rather stable nucleosomes. DNA obtained after irradiation of rats with principally different doses 8 and 100 Gy differed not only quantitatively, but also by content of the dinucleotides CpG and CpT; this suggests their origin from different sites of genome. For the first time it has been shown that exposure to low-frequency noise results in an increase of the contents of blood plasma low-molecular-weight DNA. In stroke patients blood concentrations of this DNA increased 3 days after the beginning of the acute period, and dynamics of its excretion differs in ischemic and hemorrhagic forms; in the case of ischemia low-molecular-weight DNA appears in cerebrospinal fluid. The chronic obstructive pulmonary disease in the state of remission is characterized by the decline of the level of low-molecular-weight DNA in the blood plasma unlike in the case of the chronic nonobstructive bronchitis. The clear dependence between formation and special features of the low-molecular-weight DNA fraction in blood plasma makes it possible to consider the low-molecular fraction as an universal index of apoptosis, which allows to distinguish basically different conditions of the body.
authors.forename: I N
authors.initials: IN
authors.lastname: Vasil'eva
authors.forename: V N
authors.initials: VN
authors.lastname: Zinkin
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D001991:Bronchitis / Q000097:blood / Q000175:diagnosis; D004247:DNA / Q000097:blood* / Q000134:cerebrospinal fluid*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays / Q000009:adverse effects; D006801:Humans; D020300:Intracranial Hemorrhages / Q000097:blood / Q000134:cerebrospinal fluid / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood / Q000175:diagnosis; D011833:Radiation Injuries, Experimental / Q000097:blood; D051381:Rats; D020521:Stroke / Q000097:blood / Q000134:cerebrospinal fluid / Q000175:diagnosis
chemical_list: D004247:DNA