title: Association of HHIP polymorphisms with COPD and COPD-related phenotypes in a Chinese Han population.
abstract: The hedgehog signaling pathway plays an important role in lung morphogenesis and cellular responses to lung injury. Genome-wide association studies (GWAS) and integrative genomics approaches have demonstrated the associations between HHIP polymorphisms and chronic obstructive pulmonary disease (COPD) and in non-Asian populations. Here we investigated whether HHIP polymorphisms would also be associated with COPD susceptibility and COPD-related phenotypes in a Chinese Han population. In the present case-control study a total of 680 COPD patients and 687 healthy control subjects were recruited. Six single nucleotide polymorphisms (SNPs) (rs1828591, rs13118928, rs6817273, rs10519717, rs12504628, rs13147758) were selected for genotyping. Allele frequencies and genotype distributions were compared between patients and controls. To estimate the strength of association, odds ratios (OR) (with 95% CI) were calculated and potential confounding variables were tested by using logistic regression analysis. Association between haplotypes and COPD outcome was also assessed. We identified that SNP rs12504628 was associated with FEV1/FVC ratio among cases (P=0.0460). Moreover, the HHIP SNP rs10519717 was associated with the severity of disease (adjusted P-value=0.0300). The six SNPs showed strong linkage disequilibrium (r(2)≥ 0.9). Three major haplotypes were observed but showed no significant difference between case and control groups (P=0.4532, 0.0875, and 0.3484, respectively). In conclusion, our study suggests that the HHIP gene may be involved in COPD susceptibility in Chinese Han population.
authors.affiliation: Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China. Electronic address: wangyujian88@163.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Haixia
authors.initials: H
authors.lastname: Zhou
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Xiao
authors.forename: Binmiao
authors.initials: B
authors.lastname: Liang
authors.forename: Dengxue
authors.initials: D
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.forename: Qingfei
authors.initials: Q
authors.lastname: Zeng
authors.forename: Chunju
authors.initials: C
authors.lastname: Fang
authors.forename: Zhengsheng
authors.initials: Z
authors.lastname: Rao
authors.forename: He
authors.initials: H
authors.lastname: Yu
authors.forename: Xuemei
authors.initials: X
authors.lastname: Ou
authors.forename: Yulin
authors.initials: Y
authors.lastname: Feng
mesh_terms: D000368:Aged; D000483:Alleles; D044466:Asian People; D002352:Carrier Proteins / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D008875:Middle Aged; D010641:Phenotype; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D029424:Pulmonary Disease, Chronic Obstructive / Q000235:genetics* / Q000503:physiopathology*
chemical_list: D002352:Carrier Proteins; C117596:HHIP protein, human; D008562:Membrane Glycoproteins
keywords: BMI; CI; COPD; COPD-related phenotypes; Case–control study; Chronic obstructive pulmonary disease; FEV1; FVC; GOLD; GWAS; Gene polymorphism; HHIP; HWE; Hardy–Weinberg equilibrium; LD; MAF; OR; SD; SHH; SNPs; body mass index; chronic obstructive pulmonary disease; confidence intervals; forced expiratory volume in 1s; forced vital capacity; genome-wide association studies; global Initiative for chronic obstructive lung disease; hedgehog interacting protein; linkage disequilibrium; minor allele frequency; odds ratio; single nucleotide polymorphisms; sonic hedgehog; standard deviation