title: Mammalian genes induce partially reprogrammed pluripotent stem cells in non-mammalian vertebrate and invertebrate species.
abstract: Cells are fundamental units of life, but little is known about evolution of cell states. Induced pluripotent stem cells (iPSCs) are once differentiated cells that have been re-programmed to an embryonic stem cell-like state, providing a powerful platform for biology and medicine. However, they have been limited to a few mammalian species. Here we found that a set of four mammalian transcription factor genes used to generate iPSCs in mouse and humans can induce a partially reprogrammed pluripotent stem cell (PRPSCs) state in vertebrate and invertebrate model organisms, in mammals, birds, fish, and fly, which span 550 million years from a common ancestor. These findings are one of the first to show cross-lineage stem cell-like induction, and to generate pluripotent-like cells for several of these species with in vivo chimeras. We suggest that the stem-cell state may be highly conserved across a wide phylogenetic range. DOI:http://dx.doi.org/10.7554/eLife.00036.001.
authors.affiliation: Department of Biochemistry , University of Puerto Rico Medical Sciences Campus , San Juan , Puerto Rico ; Department of Neurobiology , Duke University Medical Center , Durham , United States ; Howard Hughes Medical Institute, Duke University Medical Center , Durham , United States.
authors.forename: Ricardo Antonio
authors.initials: RA
authors.lastname: Rosselló
authors.forename: Chun-Chun
authors.initials: CC
authors.lastname: Chen
authors.forename: Rui
authors.initials: R
authors.lastname: Dai
authors.forename: Jason T
authors.initials: JT
authors.lastname: Howard
authors.forename: Ute
authors.initials: U
authors.lastname: Hochgeschwender
authors.forename: Erich D
authors.initials: ED
authors.lastname: Jarvis
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D019070:Cell Lineage; D049109:Cell Proliferation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D002642:Chick Embryo; D002678:Chimera; D004330:Drosophila; D053595:Embryonic Stem Cells / Q000378:metabolism*; D046369:Finches; D018507:Gene Expression Regulation, Developmental; D005838:Genotype; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism*; D007621:Karyotyping; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D011784:Quail; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D019098:Telomerase / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D015027:Zebrafish
chemical_list: D015415:Biomarkers; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; C489428:Myc protein, mouse; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D016271:Proto-Oncogene Proteins c-myc; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; D014157:Transcription Factors; D019098:Telomerase
keywords: Chicken; Drosophila; Mouse; Zebrafish; iPS; quail; zebra finch