title: High throughput detection of antibody self-interaction by bio-layer interferometry.
abstract: Self-interaction of an antibody may lead to aggregation, low solubility or high viscosity. Rapid identification of highly developable leads remains challenging, even though progress has been made with the introduction of techniques such as self-interaction chromatography (SIC) and cross-interaction chromatography (CIC). Here, we report a high throughput method to detect antibody clone self-interaction (CSI) using bio-layer interferometry (BLI) technology. Antibodies with strong self-interaction responses in the CSI-BLI assay also show delayed retention times in SIC and CIC. This method allows hundreds of candidates to be screened in a matter of hours with minimal material consumption.
authors.affiliation: Protein Analytics; Adimab; Lebanon, NH USA.
authors.forename: Tingwan
authors.initials: T
authors.lastname: Sun
authors.affiliation: Protein Analytics; Adimab; Lebanon, NH USA.
authors.forename: Felicia
authors.initials: F
authors.lastname: Reid
authors.affiliation: Protein Analytics; Adimab; Lebanon, NH USA.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Protein Analytics; Adimab; Lebanon, NH USA.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Protein Analytics; Adimab; Lebanon, NH USA.
authors.forename: Patricia
authors.initials: P
authors.lastname: Estep
authors.affiliation: Protein Analytics; Adimab; Lebanon, NH USA.
authors.forename: Claire
authors.initials: C
authors.lastname: Nauman
authors.affiliation: Protein Analytics; Adimab; Lebanon, NH USA.
authors.forename: Yingda
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000906:Antibodies / Q000378:metabolism*; D057166:High-Throughput Screening Assays; D007368:Interferometry
chemical_list: D000906:Antibodies
keywords: aggregation; bio-layer interferometry; developability; monoclonal antibody; self-interaction; solubility