title: Influence of several sources and amounts of iron on DNA, lipid and protein oxidative damage during anaemia recovery.
abstract: The study was designed to assess the effect of several Fe amounts and sources on haematological parameters, DNA, lipid and protein oxidative damage during the course of Fe-deficiency anaemia recovery. Peripheral DNA damage was assessed using an alkaline comet assay. The brain, liver, erythrocyte and duodenal mucosa lipid peroxidation and protein damage were assessed in control and anaemic rats after Fe repletion with three different sources (FeSO4, haem Fe, and FeSO4 + haem Fe) and amounts (45, 12, and 31 mg Fe/kg diet) of Fe: F diet, H diet or C diet, respectively. After supplying the diets, the haematological parameters studied were recovered; being remarkable is the haemoglobin increase. The DNA damage was lower in rats with the H diet, as revealed by the percentage of DNA in head, tail and Olive tail moment compared in rats with the F (P < 0.001) and C (P < 0.05) diets. Lipid peroxidation was similar in all the tissues, except in the duodenal mucosa which was lower with H and C diets (P < 0.001). The animals fed with C diet showed lower oxidative protein damage in the duodenal mucosa (P < 0.001) and was also lower in the liver and erythrocytes for H and C diets (P < 0.001). No differences were found in the brain under our experimental conditions. In conclusion, Fe supplementation with low doses of haem Fe or combined forms of non-haem and haem Fe (FeSO4 + haem) are efficient in restoring the impaired haematological parameters and prevent the evoked oxidative stress associated with Fe supplements.
authors.affiliation: Department of Physiology and Institute of Nutrition and Food Technology "José Mataix", University of Granada, 18071, Granada, Spain, javierdc@ugr.es.
authors.forename: Javier
authors.initials: J
authors.lastname: Díaz-Castro
authors.forename: Yenela
authors.initials: Y
authors.lastname: García
authors.forename: Inmaculada
authors.initials: I
authors.lastname: López-Aliaga
authors.forename: María J M
authors.initials: MJ
authors.lastname: Alférez
authors.forename: Silvia
authors.initials: S
authors.lastname: Hijano
authors.forename: Aurora
authors.initials: A
authors.lastname: Ramos
authors.forename: Margarita S
authors.initials: MS
authors.lastname: Campos
mesh_terms: D018798:Anemia, Iron-Deficiency / Q000188:drug therapy / Q000378:metabolism; D000818:Animals; D004247:DNA / Q000187:drug effects*; D005260:Female; D007501:Iron / Q000494:pharmacology* / Q000627:therapeutic use; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004247:DNA; D007501:Iron