title: An in vitro study on the secondary caries-prevention properties of three restorative materials.
abstract: BACKGROUND
  Secondary caries is considered to be the most important and common reason for the replacement of all types of restorations.
  
  
  OBJECTIVE
  The purpose of the study was to evaluate in vitro both the anticaries efficacy and marginal integrity of amalgam, glass ionomer cement, and composite resin restorations in a cariogenic condition.
  
  
  METHODS
  Class II cavities were prepared on 60 extracted teeth, which were assigned to 3 groups and restored either with amalgam, glass ionomer, or composite resin. After thermal cycling and sterilization, the teeth were set in an artificial carious environment for 10 days. The bacteria from the margin of the restorations were then collected, and microleakage was evaluated by means of dyeing. According to the locations, the demineralization of tooth tissue around the restoration was divided into 3 parts: artificial outer lesion, artificial enamel wall lesion, and artificial dentin wall lesion. The depths of the artificial outer lesion and the areas of the artificial enamel and dentin wall lesions were investigated with a confocal laser scanning microscope after all the specimens had been stained with 0.1 mM Rhodamine B. Statistical analyses consisted of 1-way ANOVA and Kruskal-Wallis tests (α=.05).
  
  
  RESULTS
  No significant difference was noted in terms of the number of bacteria around the restorations for the 3 materials; the microleakage of amalgam was the least among the 3 types of restorations; the outer enamel lesion depth around the glass ionomer cement was the lowest, and the wall lesion area around the amalgam was the smallest.
  
  
  CONCLUSIONS
  No definite inhibitory effect of fluoride releasing restorative materials (glass ionomer) was apparent on Lactobacillus acidophilus in this study. The amalgam restorations showed the best marginal integrity and the smallest artificial wall lesion among the 3 types of restorations.
authors.affiliation: Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Ninth People's Hospital, Medical College, Shanghai Jiao Tong University, PR China.
authors.forename: Guang-Yun
authors.initials: GY
authors.lastname: Lai
authors.forename: Lai-Kuan
authors.initials: LK
authors.lastname: Zhu
authors.forename: Ming-Yu
authors.initials: MY
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
mesh_terms: D002326:Cariogenic Agents / Q000009:adverse effects; D002327:Cariostatic Agents / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry; D003723:Dental Amalgam / Q000737:chemistry; D003731:Dental Caries / Q000382:microbiology / Q000517:prevention & control*; D003737:Dental Cavity Preparation / Q000145:classification; D003743:Dental Enamel / Q000382:microbiology / Q000473:pathology; D003763:Dental Leakage / Q000145:classification; D018772:Dental Marginal Adaptation; D003764:Dental Materials / Q000737:chemistry*; D003793:Dental Restoration, Permanent / Q000379:methods*; D003804:Dentin / Q000382:microbiology / Q000473:pathology; D004058:Diffusion; D005456:Fluorescent Dyes; D005459:Fluorides / Q000737:chemistry; D005899:Glass Ionomer Cements / Q000737:chemistry; D006801:Humans; D007779:Lactobacillus acidophilus / Q000302:isolation & purification; D012008:Recurrence; D012235:Rhodamines; D013295:Streptococcus mutans / Q000302:isolation & purification; D013696:Temperature; D017001:Tooth Demineralization / Q000382:microbiology / Q000473:pathology
chemical_list: D002326:Cariogenic Agents; D002327:Cariostatic Agents; D003188:Composite Resins; D003764:Dental Materials; D005456:Fluorescent Dyes; D005899:Glass Ionomer Cements; D012235:Rhodamines; D003723:Dental Amalgam; C029773:rhodamine B; D005459:Fluorides