title: Biosurfactant-producing yeasts isolated from flowering plants and bees.
abstract: The yeast strains (n=160) have been isolated from various flowering plants and bees Apis mellifera. Oil-spreading method was used to assay the ability of the isolated yeasts to produce biosurfactants. Five most active strains able to synthesize glycolipid biosurfactants produced the oil-spreading zone with diameter 3.66-50 cm The addition of oleic acid, sunflower oil and octadecane significantly increased biosurfactant activity of the studied strains. Crude biosurfactants produced by the strains Candida sp. 79a and 156a were isolated as ethyl acetate extract and proved to be a mixture of glycolipids by thin-layer chromatography.
authors.affiliation: Zabolotnv Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154. Acad. Zabolotnv St., Kyiv, MSP D03680, Ukraine.
authors.forename: O D
authors.initials: OD
authors.lastname: Ianieva
mesh_terms: D000473:Alkanes / Q000378:metabolism; D000818:Animals; D001516:Bees / Q000382:microbiology; D002175:Candida / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D035264:Flowers / Q000382:microbiology; D006017:Glycolipids / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D019301:Oleic Acid / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism; D010944:Plants / Q000382:microbiology; D000074242:Sunflower Oil; D013501:Surface-Active Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000473:Alkanes; D006017:Glycolipids; D010938:Plant Oils; D000074242:Sunflower Oil; D013501:Surface-Active Agents; D019301:Oleic Acid; C022883:octadecane