title: self-assembling GFP: a versatile tool for plant (membrane) protein analyses.
abstract: The investigation of cellular processes on the molecular level is important to understand the functional network within plant cells. self-assembling GFP has evolved to be a versatile tool for (membrane) protein analyses. Based on the autocatalytical reassembling property of the nonfluorescent strands 1-10 and 11, protein distribution and membrane protein topology can be analyzed in vivo. Here, we provide basic protocols to determine membrane protein topology in Arabidopsis thaliana protoplasts.
authors.affiliation: Cluster of Excellence Frankfurt, Center for Membrane Proteomics, Department of Biosciences, Molecular Cell Biology, Goethe University, Frankfurt, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Wiesemann
authors.forename: Lucia E
authors.initials: LE
authors.lastname: Groß
authors.forename: Manuel
authors.initials: M
authors.lastname: Sommer
authors.forename: Enrico
authors.initials: E
authors.lastname: Schleiff
authors.forename: Maik S
authors.initials: MS
authors.lastname: Sommer
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008853:Microscopy; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics / Q000302:isolation & purification; D011523:Protoplasts / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D029681:Arabidopsis Proteins; D008565:Membrane Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins