title: Overexpression of the Galega orientalis gibberellin receptor improves biomass production in transgenic tobacco.
abstract: Gibberellins (GAs) are well-known phytohormones that contribute to a wide range of plant growth and development functions including stem elongation and leaf expansion. GA receptors perceive GA and transmit signals to activate GA-regulated reactions. In this study, a GA receptor gene with homology to other leguminous plants was isolated from Galega orientalis and termed GoGID. The 1732-bp full-length GoGID gene included an open reading frame of 1035 bp encoding a peptide of 344 amino acids. Sequence analysis indicated that GoGID shares conserved HGGS motif and active amino acid sites (Ser-Asp-Val/IIe) that are essential for maintaining it GA-binding activity. GoGID mRNA expression was more abundant in leaves than in roots or stems and could be up-regulated by the exogenous hormones. Overexpression of GoGID in transgenic tobacco plants promoted plant elongation and improved biomass production. These results suggested that GoGID functions as a GA receptor to alter GA-mediated signaling. GoGID may have a role in genetic engineering for the improvement of forage crops.
authors.affiliation: Department of Forage Germplasm Resources, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Hongwen
authors.initials: H
authors.lastname: Gao
authors.forename: Jishan
authors.initials: J
authors.lastname: Jiang
authors.forename: Nikolay
authors.initials: N
authors.lastname: Dzyubenko
authors.forename: Vladimir
authors.initials: V
authors.lastname: Chapurin
authors.forename: Zan
authors.initials: Z
authors.lastname: Wang
authors.forename: Xuemin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism; D018533:Biomass; D029881:Galega / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005875:Gibberellins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010937:Plant Growth Regulators / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018514:Plant Structures / Q000378:metabolism; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D000596:Amino Acids; D005875:Gibberellins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface
keywords: Biomass; Galega orientalis L; Gibberellin receptor; Transgenic tobacco