title: Characterization of four nr5a genes and gene expression profiling for testicular steroidogenesis-related genes and their regulatory factors in response to bisphenol A in rare minnow Gobiocypris rarus.
abstract: Bisphenol A (BPA) widely used in the manufacture of numerous products is ubiquitous in aquatic environment. To explore the mechanisms of BPA-mediated actions, male rare minnow Gobiocypris rarus were exposed to BPA at concentrations of 5, 15, and 50 μg/L for 14 and 35 days in the present study. Four subtypes of nr5a gene encoding important transcription factors for steroidogenesis were characterized, and tissue distribution analysis demonstrated distinct expression profiling of the four genes in G. rarus. BPA at environmentally relevant concentration (5 μg/L) caused increase of gonadosomatic index (GSI) of male fish. In response to BPA, no obvious changes on the testis development were observed. Modulation of vtg mRNA expression by BPA suggests estrogenic and/or anti-estrogenic effects of BPA were dependent on exposed duration (14 or 35 days). Gene expression profiling for testicular steroidogenesis-related genes, sexual steroid receptors, gonadotropin receptors, and transcription factors indicates differential regulation was dependent on exposure duration and dose of BPA. The correlation analysis at mRNA level demonstrates that the BPA-mediated actions on testicular steroidogenesis might involve sex steroid hormone receptor signaling, gonadotropin/gonadotropin receptor pathway, and transcription factors such as nuclear receptor subfamily 5, group A (Nr5a), fork head box protein L2 (Foxl2).
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Cong
authors.initials: C
authors.lastname: Yuan
authors.forename: Guojun
authors.initials: G
authors.lastname: Hu
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Yao
authors.initials: Y
authors.lastname: Zheng
authors.forename: Jiancao
authors.initials: J
authors.lastname: Gao
authors.forename: Yanping
authors.initials: Y
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Zaizhao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D003530:Cyprinidae / Q000235:genetics / Q000378:metabolism*; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D008297:Male; D010636:Phenols / Q000633:toxicity*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011967:Receptors, Gonadotropin / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D029941:Fish Proteins; D010636:Phenols; D012333:RNA, Messenger; D011967:Receptors, Gonadotropin; D014157:Transcription Factors; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Gobiocypris rarus; Gonadotropin receptor; Sex steroid hormone receptor; Steroidogenesis-related gene; Transcription factor