title: Sanger dideoxy sequencing of DNA.
abstract: While the ease and reduced cost of automated DNA sequencing has largely obviated the need for manual dideoxy sequencing for routine purposes, specific applications require manual DNA sequencing. For instance, in studies of enzymes or proteins that bind or modify DNA, a DNA ladder is often used to map the site at which an enzyme is bound or a modification occurs. In these cases, the Sanger method for dideoxy sequencing provides a rapid and facile method for producing a labeled DNA ladder.
authors.affiliation: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Walker
authors.forename: Jon
authors.initials: J
authors.lastname: Lorsch
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004247:DNA
keywords: DNA labeling; Dideoxy sequencing of DNA; Primer annealing; Sanger method; Urea-PAGE