title: Enzymatic synthesis of feruloylated lysophospholipid in a selected organic solvent medium.
abstract: Feruloylated lysophospholipids (FLPs) were firstly synthesized from phosphatidylcholine (PC) and ethyl ferulate (EF) using lipase-catalysed interesterification in selected solvents at controlled water content. Kinds of lipases and single solvents were screened. Novozym 435 and toluene were found to be the suitable biocatalyst and solvent, respectively. Then tert-butanol, n-butanol, chloroform, isopropanol, acetone and DMSO were respectively added into toluene in order to increase conversion of products. The results showed that toluene/chloroform could significantly increase the conversion and the optimal combination of toluene and chloroform was 90:10 (v/v). The optimal conditions generated for FLPs production were a substrate molar ratio of 5:1 (PC/EF), a PC's hydrolytic time of 1.5h, an enzyme load of 60 mg/ml, a solvent dosage of 5 ml and a molecular sieves concentration (4Å) of 100mg/ml. Under these conditions, 40.51% of EF can be converted to FLPs, which were identified by TLC and HPLC-MS.
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, People's Republic of China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Mu
authors.forename: Hongtao
authors.initials: H
authors.lastname: Chen
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Xiu
authors.forename: Tiankui
authors.initials: T
authors.lastname: Yang
mesh_terms: D055162:Biocatalysis; D001709:Biotechnology / Q000379:methods*; D002109:Caffeic Acids / Q000737:chemistry; D004800:Enzymes, Immobilized; D004951:Esterification; D005656:Fungal Proteins; D008049:Lipase / Q000737:chemistry*; D008246:Lysophospholipids / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry
chemical_list: D002109:Caffeic Acids; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D008246:Lysophospholipids; D010713:Phosphatidylcholines; C099085:ethyl ferulate; C093667:Novozyme 435; D008049:Lipase
keywords: Enzymatic modification; Ethyl ferulate; Feruloylated lysophospholipids; Lipase; Phosphatidylcholine