title: Fucomannogalactan and glucan from mushroom Amanita muscaria: structure and inflammatory pain inhibition.
abstract: A fucomannogalactan (FMG-Am) and a (1→3), (1→6)-linked β-D-glucan (βGLC-Am) were isolated from Amanita muscaria fruiting bodies. These compounds' structures were determined using mono- and bi-dimensional NMR spectroscopy, methylation analysis, and controlled Smith degradation. FMG-Am was shown to be a heterogalactan formed by a (1→6)-linked α-D-galactopyranosyl main chain partially substituted at O-2 mainly by α-L-fucopyranose and a minor proportion of β-D-mannopyranose non-reducing end units. βGLC-Am was identified as a (1→3)-linked β-D-glucan partially substituted at O-6 by mono- and a few oligosaccharide side chains, which was confirmed after controlled Smith degradation. Both the homo- and heteropolysaccharide were evaluated for their anti-inflammatory and antinociceptive potential, and they produced potent inhibition of inflammatory pain, specifically, 91±8% (30 mg kg(-1)) and 88±7% (10 mg kg(-1)), respectively.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, CEP 81531-980, Curitiba, PR, Brazil.
authors.forename: Andrea Caroline
authors.initials: AC
authors.lastname: Ruthes
authors.forename: Elaine R
authors.initials: ER
authors.lastname: Carbonero
authors.forename: Marina Machado
authors.initials: MM
authors.lastname: Córdova
authors.forename: Cristiane Hatsuko
authors.initials: CH
authors.lastname: Baggio
authors.forename: Guilherme Lanzi
authors.initials: GL
authors.lastname: Sassaki
authors.forename: Philip Albert James
authors.initials: PA
authors.lastname: Gorin
authors.forename: Adair Roberto Soares
authors.initials: AR
authors.lastname: Santos
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacomini
mesh_terms: D000545:Amanita / Q000737:chemistry*; D000818:Animals; D005260:Female; D005557:Formaldehyde / Q000009:adverse effects; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D005685:Galactans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D007249:Inflammation / Q000150:complications; D008297:Male; D051379:Mice; D008970:Molecular Weight; D059225:Nociception / Q000187:drug effects; D010146:Pain / Q000139:chemically induced / Q000150:complications* / Q000188:drug therapy*; D012995:Solubility; D013329:Structure-Activity Relationship
chemical_list: D005685:Galactans; D005936:Glucans; D005557:Formaldehyde
keywords: (1→3), (1→6) β-d-Glucan; Amanita muscaria; Anti-inflammatory; Fucomannogalactan; Pain