title: Isolation and characterization of chicken dermis-derived mesenchymal stem/progenitor cells.
abstract: Dermis-derived mesenchymal stem/progenitor cells (DMS/PCs) were isolated from the skin tissue of 16-day-old chick embryos and then characterized by immunofluorescence and RT-PCR. We found that primary DMS/PCs could be expanded for 15 passages. Expression of β -integrin, CD44, CD71, and CD73 was observed by immunofluorescence and RT-PCR. Passage 3 DMS/PCs were successfully induced to differentiate into osteoblasts, adipocytes, and neurocytes. The results indicate the potential for multilineage differentiation of DMS/PCs that may represent an ideal candidate for cellular transplantation therapy.
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Gao
authors.forename: Chunyu
authors.initials: C
authors.lastname: Bai
authors.forename: Hui
authors.initials: H
authors.lastname: Xiong
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Shan
authors.forename: Linsheng
authors.initials: L
authors.lastname: Huang
authors.forename: Yuehui
authors.initials: Y
authors.lastname: Ma
authors.forename: Weijun
authors.initials: W
authors.lastname: Guan
mesh_terms: D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D002469:Cell Separation / Q000379:methods*; D048430:Cell Shape; D002478:Cells, Cultured; D002645:Chickens; D020405:Dermis / Q000166:cytology*; D005786:Gene Expression Regulation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D055495:Neurogenesis / Q000235:genetics; D010012:Osteogenesis / Q000235:genetics
chemical_list: D000954:Antigens, Surface