title: Differential expression of p53 family proteins in colorectal adenomas and carcinomas: Prognostic and predictive values.
abstract: We studied the contribution of p53 family proteins and their isoforms to the development and progression of colorectal carcinoma in relation to VEGF.
  
  
  
  p53, p63, p73 and VEGF proteins were assessed in 45 colorectal adenomas (CRAs), 80 carcinomas (CRCs) and 36 normal colonic tissue samples (NCT) by immunohistochemistry. Different p63 and p73 isoforms were assessed by RT-PCR. Aberrant protein and RNA expressions were correlated to patients' characteristics, disease free and overall survival (DFS and OS).
  
  
  
  p53, p63, p73 and VEGF proteins were detected in 22.2%, 73.3%, 33.3%, 46.7% CRAs; in 68.8%, 38.8%, 62.5%, 62.5% CRCs and 16.7%, 83.3%; 13.9%, 41.7% NCT (p<0.05 except for VEGF). Commonest isoforms were TAp63α, ΔNp63, TAp73α in CRA and ΔNp63, TAp63α, ΔNp73, TAp73β in CRC. Significant correlations were found between aggressive tumor phenotypes and aberrations in p73, p53, p63, VEGF. DFS correlated with advanced stage, p73 and VEGF aberrations. While advanced stage, positive lymph nodes, p73 and p53 correlated with OS. Prognosis was worse in patients with aberrant p63 and p73 than in those with normal p63 and p73 expression regardless of p53 gene status (p⟨0.05).
  
  
  
  p53 family proteins and VEGF play a pivotal role in colorectal carcinogenesis. p53 prognostic potential is augmented by p73 and p63 aberrations indicating a synergistic effect between the three family members. Nodal status, stage, p73, VEGF and p53 could be used as predictors of DFS and OS.
authors.affiliation: Pathology Department, National Cancer Institute, Cairo University, Cairo, Egypt. chaya2000@hotmail.com.
authors.forename: Abeer A
authors.initials: AA
authors.lastname: Bahnassy
authors.affiliation: Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt.
authors.forename: Abdel-Rahman N
authors.initials: AR
authors.lastname: Zekri
authors.affiliation: Medical Oncology Department, National Cancer Institute, Cairo University, Cairo, Egypt.
authors.forename: Salem E
authors.initials: SE
authors.lastname: Salem
authors.affiliation: Pathology Department, National Cancer Institute, Cairo University, Cairo, Egypt.
authors.forename: Amany A
authors.initials: AA
authors.lastname: Abou-Bakr
authors.affiliation: Pathology Department, National Cancer Institute, Cairo University, Cairo, Egypt.
authors.forename: Mona A
authors.initials: MA
authors.lastname: Sakr
authors.affiliation: Medical Oncology Department, National Cancer Institute, Cairo University, Cairo, Egypt.
authors.forename: Ayman G
authors.initials: AG
authors.lastname: Abdel-Samiaa
authors.affiliation: Radiotherapy Department, National Cancer Institute, Cairo University, Cairo, Egypt.
authors.forename: Manal
authors.initials: M
authors.lastname: Al-Bradei
mesh_terms: D000236:Adenoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D003106:Colon / Q000378:metabolism / Q000473:pathology; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011237:Predictive Value of Tests; D011379:Prognosis; D011446:Prospective Studies; D000072160:Tumor Protein p73; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: C081876:CKAP4 protein, human; D004268:DNA-Binding Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; C000606460:TP73 protein, human; D000072160:Tumor Protein p73; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; D042461:Vascular Endothelial Growth Factor A; C000606369:delta Np73 protein, human