title: Molecular characterisation and the light-dark regulation of carotenoid biosynthesis in sprouts of tartary buckwheat (Fagopyrum tataricum Gaertn.).
abstract: Seven partial-length cDNAs and 1 full-length cDNA that were involved in carotenoid biosynthesis and 2 partial-length cDNAs that encoded carotenoid cleavage dioxygenases were first isolated and characterised in 2 tartary buckwheat cultivars (Fagopyrum tataricum Gaertn.), Hokkai T8 and Hokkai T10. They were constitutively expressed at high levels in the leaves and flowers, where carotenoids are mostly distributed. During the seed development of tartary buckwheat, an inverse correlation between transcription level of carotenoid cleavage dioxygenase and carotenoid content was observed. The light-grown sprouts exhibited higher levels of expression of carotenoid biosynthetic genes in T10 and carotenoid content in both T8 and T10 compared to the dark-grown sprouts. The predominant carotenoids in tartary buckwheat were lutein and β-carotene, and very abundant amounts of these carotenoids were found in light-grown sprouts. This study might broaden our understanding of the molecular mechanisms involved in carotenoid biosynthesis and indicates targets for increasing the production of carotenoids in tartary buckwheat.
authors.affiliation: Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-Gu, Daejeon 305-764, South Korea.
authors.forename: Pham Anh
authors.initials: PA
authors.lastname: Tuan
authors.forename: Aye Aye
authors.initials: AA
authors.lastname: Thwe
authors.forename: Jae Kwang
authors.initials: JK
authors.lastname: Kim
authors.forename: Yeon Bok
authors.initials: YB
authors.lastname: Kim
authors.forename: Sanghyun
authors.initials: S
authors.lastname: Lee
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Park
mesh_terms: D053898:Biosynthetic Pathways / Q000528:radiation effects; D002338:Carotenoids / Q000096:biosynthesis*; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism; D019612:Fagopyrum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D018525:Germination / Q000528:radiation effects; D008027:Light; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism / Q000528:radiation effects
chemical_list: D010940:Plant Proteins; D002338:Carotenoids; D049308:Dioxygenases; C431939:carotenoid cleavage dioxygenase 1
keywords: 9-cis-epoxycarotenoid dioxygenase; ABA; CCD; CHXB; CHXE; Carotenoids; DAS; DEPC; GGDP; Gene characterization; HPLC; LCYB; LCYE; NCED; PDS; PSY; Seed; Sprout; Tartary buckwheat; ZDS; ZEP; abscisic acid; carotenoid cleavage dioxygenase; days after sowing; diethylpyrocarbonate; geranylgeranyl diphosphate; high-performance liquid chromatography; lycopene β-cyclase; lycopene ε-cyclase; phytoene desaturase; phytoene synthase; zeaxanthin epoxidase; β-ring carotene hydroxylase; ε-ring carotene hydroxylase; ξ-carotene desaturase