title: [Comparison between icariin and genistein in osteogenic activity of marrow stromal cells].
abstract: OBJECTIVE
  To compare the effect of icariin and genistein in the osteogenic differentiation of rat bone marrow stromal cells (rBMSC).
  
  
  METHODS
  Rat marrow stromal cells were seperated in vitro, and the optimal concentration of genisten and icriin were screened. Genistein and icariin with the concentration of 1 x 10(-5) mol x L(-1) were adopted to intereven rBMSCs cultured in vitro. Alkaline phosphatase (ALP) was determined at 3, 6, 9, 12,15 d after intervention; calcified nodule was detected with alizarin red staining at 12 d; OXS, Runx-2, bone morphogenetic protein (BMP-2) and Collagen-I mRNA expression were observed with Real-time RT-PCR at 12, 24, 48, 72, 96 h.
  
  
  RESULTS
  Genistein and icariin with the concentration of 1 x 10(-5) mol x L(-1) could increase the activity of ALP and the content of Ca, regulate OXS, BMP-2, Runx-2 and Collagen-I mRNA expression.
  
  
  CONCLUSIONS
  Icariin showed a stronger effect in improving the osteogenic differentiation of rat bone marrow stromal cells than genistein.
authors.affiliation: Institute of Orthopaedics, Lanzhou General Hospital of PLA, Lanzhou 730050, China. zhoujian715412@126.com
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.forename: Ke-Ming
authors.initials: KM
authors.lastname: Chen
authors.forename: Bao-Feng
authors.initials: BF
authors.lastname: Ge
authors.forename: Xiao-Ni
authors.initials: XN
authors.lastname: Ma
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Guo
authors.forename: Kui
authors.initials: K
authors.lastname: Cheng
authors.forename: Yu-Hai
authors.initials: YH
authors.lastname: Gao
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Yan
authors.forename: Wen-Gui
authors.initials: WG
authors.lastname: Shi
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005419:Flavonoids / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism
chemical_list: D055396:Bone Morphogenetic Protein 2; D005419:Flavonoids; D016212:Transforming Growth Factor beta; D019833:Genistein; D000469:Alkaline Phosphatase; C056599:icariin