title: Association of Estrogen Receptor α Genes PvuII and XbaI Polymorphisms with Type 2 Diabetes Mellitus in the Inpatient Population of a Hospital in Southern Iran.
abstract: BACKGROUND
  Estrogen plays a fundamental role in the pathogenesis of type 2 diabetes mellitus (T2DM). Very few studies have shown the association between estrogen receptor α (ERα), PvuII and XbaI gene polymorphisms with T2DM in both men and women. We evaluated the hypothesis that PvuII and XbaI polymorphisms of ERα gene may be associated with T2DM in adult.
  
  
  METHODS
  From spring of 2010 to the fall of 2011, a case-control study was performed at clinical centers of Jahrom University of Medical Sciences. We included 174 patients with T2DM including men and women and 174 age, sex, and body mass index frequency-matched health controls. We analyzed the PvuII and XbaI polymorphisms of ERα by using the polymerase chain reaction-based restriction fragment length polymorphism method.
  
  
  RESULTS
  No significant differences between demographic characteristics of control and patients groups were observed. Allele frequencies of both PvuII and XbaI polymorphisms were significantly different between patients and control subjects (P=0.014 vs. P=0.002, respectively). When the group was separated into women and men, logistic regression analysis of genotype distribution of PvuII (pp vs. Pp+PP) in both sexes revealed that there was no significant association of PvuII genotype with men (odds ratio [OR], 1.67; confidence interval [CI], 0.86 to 3.28; P=0.89) and women (OR, 0.96; CI, 0.53 to 1.74; P=0.12).
  
  
  CONCLUSIONS
  PvuII and XbaI polymorphisms in ERα are related with T2DM in the inpatient population.
authors.affiliation: Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Mohammadi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Pourahmadi
authors.forename: Mohadeseh
authors.initials: M
authors.lastname: Mosalanejad
authors.forename: Houshang
authors.initials: H
authors.lastname: Jamali
authors.forename: Mohamed Amin
authors.initials: MA
authors.lastname: Ghobadifar
authors.forename: Saeideh
authors.initials: S
authors.lastname: Erfanian
keywords: Diabetes mellitus, type 2; Estrogen receptor alpha; Genetic; Humans; PvuII; XbaI