title: Development of a new method to prepare nano-/microparticles loaded with extracts of Azadirachta indica, their characterization and use in controlling Plutella xylostella.
abstract: Biodegradable nanoparticles have been widely explored as carriers for controlled delivery of therapeutic molecules; however, studies describing the development of nanoparticles as carriers for biopesticide products are few. In this work, a new method to prepare nanoparticles loaded with neem (Azadirachta indica) extracts is presented. In this study, nanoparticles were formulated as colloidal suspension and (spray-dried) powder and characterized by evaluating pH, particle size, zeta potential, morphology, absolute recovery, and entrapment efficiency. A high-performance liquid chromatography method was used for nanoparticle characterization. The best formulations presented absolute recovery and entrapment efficiencies of approximately 100% and a release profile based on swelling and relaxation of the polymer or polymer erosion. The biological data of the formulated products against Plutella xylostella showed 100% larval mortality. The nanoparticle information improved the stability of neem products against ultraviolet radiation and increased their dispersion in the aqueous phase.
authors.affiliation: Department of Chemistry, Federal University of São Carlos , Rod. Washington Luiz, Km 235, PB 676, São Carlos, SP 13.565-905, Brazil.
authors.forename: Moacir Rossi
authors.initials: MR
authors.lastname: Forim
authors.forename: Eveline Soares
authors.initials: ES
authors.lastname: Costa
authors.forename: Maria Fátima das Graças Fernandes
authors.initials: MF
authors.lastname: da Silva
authors.forename: João Batista
authors.initials: JB
authors.lastname: Fernandes
authors.forename: Janaina Marques
authors.initials: JM
authors.lastname: Mondego
authors.forename: Arlindo Leal
authors.initials: AL
authors.lastname: Boiça Junior
mesh_terms: D000818:Animals; D031601:Azadirachta / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004337:Drug Carriers / Q000737:chemistry*; D004355:Drug Stability; D007300:Insect Control; D007306:Insecticides / Q000737:chemistry* / Q000494:pharmacology; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D009036:Moths / Q000187:drug effects* / Q000254:growth & development; D053758:Nanoparticles / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D004337:Drug Carriers; D007306:Insecticides; D010936:Plant Extracts