title: Highly efficient extraction and lipase-catalyzed transesterification of triglycerides from Chlorella sp. KR-1 for production of biodiesel.
abstract: We developed a method for the highly efficient lipid extraction and lipase-catalyzed transesterification of triglyceride from Chlorella sp. KR-1 using dimethyl carbonate (DMC). Almost all of the total lipids, approximately 38.9% (w/w) of microalgae dry weight, were extracted from the dried microalgae biomass using a DMC and methanol mixture (7:3 (v/v)). The extracted triglycerides were transesterified into fatty acid methyl esters (FAMEs) using Novozyme 435 as the biocatalyst in DMC. Herein, DMC was used as the reaction medium and acyl acceptor. The reaction conditions were optimized and the FAMEs yield was 293.82 mg FAMEs/g biomass in 6 h of reaction time at 60 °C in the presence of 0.2% (v/v) water. Novozyme 435 was reused more than ten times while maintaining relative FAMEs conversion that was greater than 90% of the initial FAMEs conversion.
authors.affiliation: Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Ok Kyung
authors.initials: OK
authors.lastname: Lee
authors.affiliation: Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Young Hyun
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Clean Fuel Department, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea.
authors.forename: Jeong-Geol
authors.initials: JG
authors.lastname: Na
authors.affiliation: Clean Fuel Department, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea. Electronic address: ykoh@kier.re.kr.
authors.forename: You-Kwan
authors.initials: YK
authors.lastname: Oh
authors.affiliation: Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Republic of Korea. Electronic address: eunylee@khu.ac.kr.
authors.forename: Eun Yeol
authors.initials: EY
authors.lastname: Lee
mesh_terms: D055162:Biocatalysis; D056804:Biofuels; D002708:Chlorella / Q000378:metabolism*; D002849:Chromatography, Gas; D004951:Esterification; D008049:Lipase / Q000378:metabolism*; D014280:Triglycerides / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D056804:Biofuels; D014280:Triglycerides; D008049:Lipase
keywords: Chlorella sp. KR-1; Dimethyl carbonate; Fatty acid methyl esters; Microalgae; Novozyme 435