title: MiR-216a and miR-216b as markers for acute phased pancreatic injury.
abstract: MicroRNAs (miRNAs) are endogenous small RNAs (length, 18-ss23 nucleotides) that regulate gene expression. Recently, plasma miRNAs have been investigated as biomarkers for various diseases. In the present study, we explored cell- or tissue-specific miRNAs and assessed the applicability of miRNA profiling for identifying biomarkers of tissue injuries. miRNA analyses in various human and rat tissues identified several candidate miRNAs with possible tissue-specific expression, some of which have already been reported. In the present study, we focused on pancreas-specific miRNAs, miR-216a and miR-216b. Laser microdissection revealed that miR-216a and 216b were predominantly expressed in acinar cells of the pancreas as compared to Langerhans' islet. Plasma concentrations of miR-216a and miR-216b considerably increased in a rat model of L-arginineinduced acute pancreatitis. The current results have confirmed that miRNA expression profiling in various cells is useful for providing biomarkers for cell- or tissue-specific injuries.
authors.affiliation: Department of Genomic Medicine, National Cerebral and Cardiovascular Center, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan. ko-end@ncvc.go.jp
authors.forename: Kosuke
authors.initials: K
authors.lastname: Endo
authors.forename: Huachun
authors.initials: H
authors.lastname: Weng
authors.forename: Naoko
authors.initials: N
authors.lastname: Kito
authors.forename: Yasue
authors.initials: Y
authors.lastname: Fukushima
authors.forename: Naoharu
authors.initials: N
authors.lastname: Iwai
mesh_terms: D000208:Acute Disease; D000818:Animals; D015415:Biomarkers; D016000:Cluster Analysis; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D009928:Organ Specificity / Q000235:genetics; D010195:Pancreatitis / Q000097:blood / Q000235:genetics*; D051381:Rats; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; C575482:MIRN216 microRNA, human; D035683:MicroRNAs