title: Repeatability and sensitivity of high resolution blood volume mapping in mouse kidney disease.
abstract: OBJECTIVE
  To evaluate the repeatability of MRI-derived relative blood volume (RBV) measurements in mouse kidneys across subjects and days and to evaluate sensitivity of this approach to renal pathology.
  
  
  METHODS
  A 7 Tesla MRI system and an intravascular iron-oxide contrast agent were used to acquire spin-echo-based renal RBV maps in 10 healthy mice on 2 consecutive days. Renal RBV maps were also acquired in the Alport and unilateral ureteral obstruction mouse models of renal disease.
  
  
  RESULTS
  The average renal RBV measured on consecutive days was 19.97 ± 1.50 and 19.86 ± 1.62, yielding a concordance correlation coefficient of 0.94, indicating that this approach is highly repeatable. In the disease models, the RBV values were regionally dissimilar and substantially lower than those found in control mice.
  
  
  CONCLUSIONS
  In vivo renal iron-oxide-based RBV mapping in mice complements the physiological information obtained from conventional assays of kidney function and could shed new insights into the pathological mechanisms of kidney disease.
authors.affiliation: Vanderbilt University Institute of Imaging Science, Nashville, Tennessee, USA; Department of Radiology and Radiological Sciences, Vanderbilt University, Nashville, Tennessee, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Rosie T
authors.initials: RT
authors.lastname: Jiang
authors.forename: Mohammed Noor
authors.initials: MN
authors.lastname: Tantawy
authors.forename: Dorin B
authors.initials: DB
authors.lastname: Borza
authors.forename: Keiko
authors.initials: K
authors.lastname: Takahashi
authors.forename: John C
authors.initials: JC
authors.lastname: Gore
authors.forename: Raymond C
authors.initials: RC
authors.lastname: Harris
authors.forename: Takamune
authors.initials: T
authors.lastname: Takahashi
authors.forename: C Chad
authors.initials: CC
authors.lastname: Quarles
mesh_terms: D000465:Algorithms; D000818:Animals; D001783:Blood Flow Velocity; D001810:Blood Volume; D001811:Blood Volume Determination / Q000379:methods*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods; D007674:Kidney Diseases / Q000175:diagnosis* / Q000503:physiopathology*; D007677:Kidney Function Tests / Q000379:methods*; D018810:Magnetic Resonance Angiography / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012079:Renal Circulation; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
keywords: renal blood volume; renal function; renal models of disease; renal perfusion; susceptibility contrast MRI