title: [FA-MNP-MMP-9-ASODN complex construction and the study of its FA molecular targeting ability].
abstract: OBJECTIVE
  To construct FA targeted magnetic nanocomplex (FA-MNP-MMP-9-ASODN) loading matrix metalloproteinase 9 (MMP-9) antisense oligonucleotide (ASODN) and evaluate its targeting capacity and efficiency of gene transfection to folate receptor (FR) positive NPC.
  
  
  METHODS
  FA-MNP-MMP-9-ASODN was constructed by MMP-9-ASODN coupling with FA-MNP prepared by our research team through the aldehyde-ammonia condensation reaction. To analyze the feasibility of ASODN coupling with nanocarrier agarose gel electrophoresis. Two kinds of HNE-1 and CNE-2 cells and implanted tumors phagocytosis of FA-MNP-MMP-9-ASODN were observed by MRI on tumor-bearing nude mice, iron staining and TEM. To analyze gene transfection of the vector by observing FITC in the cell.
  
  
  RESULTS
  The electrophoresis results revealed ASODN successfully coupling with FA-MNP. HNE-1 cell can effectively ingest the nanocomposite,with more FITC in the cell, but CNE-2 cell had not uptake for the nanocomposite, with no FITC in the cell. By comparing with CNE-2 tumor, HNE-1 tumor also can efficiently swallow the nanocomposite.
  
  
  CONCLUSIONS
  FA-MNP-MMP-9-ASODN nanocomplex is constructed successfully with good FA targeting ability and gene transfection.
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, People's Hospital of Jiangxi Province, Nanchang, 330006, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Minqiang
authors.initials: M
authors.lastname: Xie
authors.forename: Dong
authors.initials: D
authors.lastname: Ma
authors.forename: Yiming
authors.initials: Y
authors.lastname: Xu
authors.forename: Hongzheng
authors.initials: H
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
mesh_terms: D000818:Animals; D005492:Folic Acid / Q000235:genetics*; D005822:Genetic Vectors; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053761:Nanocomposites; D009368:Neoplasm Transplantation; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D014162:Transfection
chemical_list: D016376:Oligonucleotides, Antisense; D005492:Folic Acid; D020780:Matrix Metalloproteinase 9