title: Effect of administration of bifidobacteria on intestinal microbiota in low-birth-weight infants and transition of administered bifidobacteria: a comparison between one-species and three-species administration.
abstract: The effects of administration of bifidobacteria on the intestinal microbiota in low-birth-weight infants, and the transition of each strain of administered bifidobacteria were investigated. A single strain of Bifidobacterium breve M-16V (5 × 10(8); one-species group) or a mixture of three species composed of B. breve M-16V, Bifidobacterium longum subsp. infantis M-63 and B. longum subsp. longum BB536 (5 × 10(8) of each strain; three-species group) were administered daily for 6 weeks. Bifidobacterial administration significantly increased the detection rates and cell numbers of bifidobacteria in the feces (weeks 1-6). The proportion of bifidobacteria was significantly higher in the one-species group at weeks 1-4, and in the three-species group at weeks 1-6 compared with the control group. Furthermore, the proportion of bifidobacteria in the three-species group was significantly higher than that in the one-species group at weeks 1 and 6. The proportion of infants with bifidobacteria-predominant microbiota was significantly higher in the three-species group than in the control group during the test period. The detection rates of Clostridium were lower in the bifidobacteria-administered groups. The proportions of Enterobacteriaceae were significantly lower in the three-species group compared to the other groups (weeks 4 and 6). Among the three strains administered, B. breve M-16V and Bifidobacterium infantis M-63 were detected in 85% or more of the infants during the administration period, while B. longum BB536 was detected in 40% or less. Compared with administration of one species, administration of three species of bifidobacteria resulted in earlier formation of a bifidobacteria-predominant fecal microbiota and maintenance of this microbiota.
authors.affiliation: Ishizeki Clinic, Saitama, Japan.
authors.forename: Shinobu
authors.initials: S
authors.lastname: Ishizeki
authors.forename: Masaoki
authors.initials: M
authors.lastname: Sugita
authors.forename: Masaaki
authors.initials: M
authors.lastname: Takata
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yaeshima
mesh_terms: D058491:Bacterial Load; D001644:Bifidobacterium / Q000502:physiology*; D058448:Biota; D004032:Diet / Q000379:methods*; D005243:Feces / Q000382:microbiology; D006801:Humans; D007230:Infant, Low Birth Weight; D007231:Infant, Newborn; D019936:Probiotics / Q000008:administration & dosage*; D016896:Treatment Outcome
keywords: Bifidobacteria; Intestinal microbiota; Premature infant