title: Explanatory chapter: how plasmid preparation kits work.
abstract: To isolate plasmid DNA from bacteria using a commercial plasmid miniprep kit (if interested, compare this protocol with Isolation of plasmid DNA from bacteria).
authors.affiliation: Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: laurakoontz@gmail.com.
authors.forename: Laura
authors.initials: L
authors.lastname: Koontz
mesh_terms: D004269:DNA, Bacterial / Q000302:isolation & purification*; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D010957:Plasmids / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic
chemical_list: D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic
keywords: Binding buffer through column; DNA elution; Lyse bacteria; Neutralization; Pellet bacteria; Plasmid preparation kit; Washing column