title: Characterisation and determination of in vitro antioxidant potential of betalains from Talinum triangulare (Jacq.) Willd.
abstract: Talinum triangulare (Jacq.) Willd is an erect, succulent, perennial herb belonging to the family Portulacaceae. Under extreme environmental conditions, the plant produces betalain pigments which get accumulated in the stem region. Hence, in the present study, the betaxanthin and betacyanin patterns from different samples of T. triangulare have been investigated by applying high-performance liquid chromatography photo-diode array detection (HPLC-PDA) coupled with positive ion electro-spray mass spectrometry. Two betacyanins and two betaxanthins were identified in aqueous methanolic extract of flower, stem and leaf. Betanin, isobetanin, immonium conjugates of betalamic acid with dopamine and tyrosine were elucidated. The total betalain content was estimated by photometric analysis. In vitro antioxidant activity for the betalain extract determined by various methods revealed potent scavenging ability. The current work may possibly be considered beneficial in utilisation of the plant T. triangulare as a natural colourant in food and beverage industries.
authors.affiliation: T.A.L. Samy Centre for Plant Tissue Culture and Molecular Biology, Department of Plant Biology and Biotechnology, Loyola College, Nungambakkam, Chennai 600 034, Tamil Nadu, India.
authors.forename: J
authors.initials: J
authors.lastname: Swarna
authors.forename: T S
authors.initials: TS
authors.lastname: Lokeswari
authors.forename: M
authors.initials: M
authors.lastname: Smita
authors.forename: R
authors.initials: R
authors.lastname: Ravindhran
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D050858:Betalains / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005505:Food Coloring Agents / Q000737:chemistry*; D019684:Magnoliopsida / Q000737:chemistry*; D013058:Mass Spectrometry; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D005505:Food Coloring Agents; D010936:Plant Extracts; D050858:Betalains
keywords: Antioxidant; Betalains; HPLC–MS; Portulacaceae; Waterleaf