title: Oxidative stress in leaves of two olive cultivars under freezing conditions.
abstract: Olive is one of the most important cultivated Mediterranean plants. In order to determine the differences in frost resistance of two, two-year-old olive cultivars (Olea europaea cv. Leccino and cv. Oblica) growing on different types of nutrient substrates (soil and coconut fibres), the trees were exposed to low temperature (-5 °C) in the dark. It was shown that low temperature caused an increase in H2O2 concentration, level of lipid peroxidation and carbonyl protein content in both cultivars and on both nutrient substrates, respectively. The CAT and APX activities significantly varied depending on the cultivar, the nutrient substrate type and the time of exposure to low temperature. Cv. Oblica and cv. Leccino growing on coconut fibres showed a better antioxidative response to low temperature probably due to the higher nitrogen and phosphorus concentration established in this type of nutrient substrate. That positive antioxidative response determined on coconut fibres was more pronounced in leaves of cv. Leccino.
authors.affiliation: University of J. J. Strossmayer in Osijek Department of Biology Cara Hadrijana 8/A HR-31000 Osijek Croatia.
authors.forename: Tanja Žuna
authors.initials: TŽ
authors.lastname: Pfeiffer
authors.forename: Ivna
authors.initials: I
authors.lastname: Štolfa
authors.forename: M
authors.initials: M
authors.lastname: Žanić
authors.forename: N
authors.initials: N
authors.lastname: Pavičić
authors.forename: Vera
authors.initials: V
authors.lastname: Cesar
authors.forename: H
authors.initials: H
authors.lastname: Lepeduš
mesh_terms: D060387:Ascorbate Peroxidases / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism*; D005615:Freezing; D031658:Olea / Q000201:enzymology*; D018384:Oxidative Stress; D018515:Plant Leaves / Q000201:enzymology; D013045:Species Specificity
chemical_list: D060387:Ascorbate Peroxidases; D002374:Catalase