title: Zwitterionic hydrophilic interaction chromatography coupled with post-column derivatization for the analysis of glutathione in wine samples.
abstract: In this study the development, validation and application of a new chromatographic method for the determination of glutathione (GSH) in wine samples is presented. The separation of the GSH was carried out using a sulfobetaine-based hydrophilic interaction chromatography (HILIC) analytical column whereas its detection was carried out spectrofluorimetrically (λext/λem=340/455 nm) after post-column derivatization with o-phthalaldehyde. GSH was separated efficiently from matrix endogenous compounds of wines by using a mobile phase of 15 mmol L(-1) CH3COONH4 (pH=2.5)/CH3CN, 35/65% (v/v). The parameters of the post-column reaction (pH, amount concentration of the reagent and buffer solution, flow rate, length of the reaction coil) were investigated. The linear determination range for GSH was 0.25-5.0 μmol L(-1) and the LOD was 19 nmol L(-1). No matrix effect was observed, while the accuracy was evaluated with recovery experiments and was ranged between 89% and 108%.
authors.affiliation: Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: Constantinos K
authors.initials: CK
authors.lastname: Zacharis
authors.forename: Paraskevas D
authors.initials: PD
authors.lastname: Tzanavaras
authors.forename: Theano D
authors.initials: TD
authors.lastname: Karakosta
authors.forename: Demetrius G
authors.initials: DG
authors.lastname: Themelis
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005978:Glutathione / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007477:Ions / Q000737:chemistry; D014920:Wine / Q000032:analysis*
chemical_list: D007477:Ions; D005978:Glutathione
keywords: Glutathione; Hydrophilic interaction chromatography; Post-column derivatization; Wines; o-Phthalaldehyde