title: Simultaneous isolation of seven compounds from Glehnia littoralis roots by off-line overpressured layer chromatography guided by a TLC antioxidant autographic assay.
abstract: A combinative method using overpressured layer chromatography (OPLC) and TLC bioautography against O2(•-) was developed to separate antioxidants from Glehnia littoralis roots. Through target-directed isolation by the TLC bioautographic method, seven compounds including five antioxidants were rapidly isolated by OPLC and identified as 1-linoloyl-3-palmitoylglycerol, facarindiol, panaxynol, isoimperatorin, β-sitosterol, scopoletin, and umbelliferone from G. littoralis roots. This OPLC method is a very suitable separation technique for light-sensitive polyacetylenes (panaxynol and facarindiol) with higher isolated yields compared to conventional open column chromatography. This is the first report on the separation of polyacetylenes by OPLC.
authors.affiliation: Key Laboratory of Standardization of Chinese Medicines of Ministry of Education, The Shanghai Key Laboratory for Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, P. R. China; Department of Pharmacognosy, China Pharmaceutical University, Nanjing, P. R. China.
authors.forename: Xing
authors.initials: X
authors.lastname: Su
authors.forename: Xiangkun
authors.initials: X
authors.lastname: Li
authors.forename: Hongxun
authors.initials: H
authors.lastname: Tao
authors.forename: Jinge
authors.initials: J
authors.lastname: Zhou
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.forename: Guixin
authors.initials: G
authors.lastname: Chou
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Cheng
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D019661:Apiaceae / Q000737:chemistry*; D001345:Autoradiography; D002855:Chromatography, Thin Layer; D018517:Plant Roots / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry / Q000302:isolation & purification*; D011312:Pressure
chemical_list: D000975:Antioxidants; D053279:Polyynes
keywords: Antioxidants; Bioautography; Glehnia littoralis; Overpressured layer chromatography