title: [Effect of paeonol on LPS-induced rat vascular endothelial cell adhesion reaction].
abstract: OBJECTIVE
  To observe the effect of Paeonol (Pae) on lipopolysaccharide (LPS) induced rat mononuclear cells (MCs) adhesion to vascular endothelial cells (VECs) and provide basis foundation for inflammatary mechanisms of Pae against atherosclerosis.
  
  
  METHODS
  Rat vascular endothelial cells were isolated with tissue predigested adherent method. LPS was used as stimulator to induce VEC injury. Serum containing Pae obtained from healthy rats which were given Pae in intragastric. RP-HPLC method was used for detecting the concentration of Pae in serum. MTT assay was used to determine the protective effect of Pae on injured VECs. Rose Bengal Staining was used to detect the effect of Pae on LPS-induced MCs adhesion to VECs.
  
  
  RESULTS
  LPS induced rat MCs adhesion to VECs. The effect was the strongest when the concentration was 10 ng/mL and incubated with VECs for 5 h. Pae in concentration of 2. 5,5 and 10 microg/mL and incubated for 24 h could effectively inhibit the adhesion and improve the survival rate of LPS injured VECs significantly.
  
  
  CONCLUSIONS
  LPS can damage VECs. Pae could protect VECs from LPS injury via inhibiting MCs adhesion to VECs and improving the VEC survival rate.
authors.affiliation: Key Laboratory of Xin'an Medicine, Ministry of Education/Department of Pharmacy, Anhui University of Traditional Chinese Medicine/Key Laboratory of Research and Development for Chinese Traditional Medicine, Hefei 230031, China. chenjunjun8812@sina.com
authors.forename: Jun-Jun
authors.initials: JJ
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Dai
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
mesh_terms: D000098:Acetophenones / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D050197:Atherosclerosis / Q000188:drug therapy* / Q000473:pathology; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000187:drug effects* / Q000473:pathology; D007249:Inflammation / Q000473:pathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D009000:Monocytes / Q000187:drug effects*; D029596:Paeonia / Q000737:chemistry; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000098:Acetophenones; D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D020011:Protective Agents; C013638:paeonol