title: Shoot-tip cryopreservation by droplet vitrification of Byrsonima intermedia A. Juss.: a woody tropical and medicinal plant species from Brazilian cerrado.
abstract: Cryopreservation of plant species is poorly investigated in Brazil. The aim of this study was to cryopreserve Byrsonima intermedia shoot apical meristems through droplet vitrification. A culture medium for shoot-tips growth was established using the Woody Plant Medium supplemented with 2.22 uM 6-benzylaminopurine. Excised shoot-tips were subjected to pre-culture and/or post-culture treatments on Murashige and Skoog medium with 0.3 M sucrose for 24 h prior dehydration on PVS2 at 0°C for 15, 30 or 45 minutes prior to plunging in liquid nitrogen. The effect of 15 days of shoot pre-growth on a high osmotic medium (0.3 M sucrose or 0.21 M sorbitol + 0.09 M sucrose) prior to meristem excision and cryopreservation was also investigated. Pre-culturing shoot-tips on 0.3 M sucrose for 24 h prior to cryopreservation increased the regrowth level after thawing to 90%. Shoot-tips excised from shoots pre-grown on MS + 0.21 M sorbitol + 0.09 M sucrose for 15 days presented a satisfactory regrowth level (67%).
authors.affiliation: Departamento de Biologia, Universidade Federal de Lavras, Brasil. lucoutsilva@yahoo.com.br
authors.forename: L C
authors.initials: LC
authors.lastname: Silva
authors.forename: R
authors.initials: R
authors.lastname: Paiva
authors.forename: R
authors.initials: R
authors.lastname: Swennen
authors.forename: E
authors.initials: E
authors.lastname: Andre
authors.forename: B
authors.initials: B
authors.lastname: Panis
mesh_terms: D001593:Benzyl Compounds; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000378:metabolism; D007701:Kinetin / Q000378:metabolism; D029581:Malpighiaceae / Q000187:drug effects / Q000254:growth & development*; D018519:Meristem / Q000187:drug effects / Q000254:growth & development; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development*; D010946:Plants, Medicinal / Q000187:drug effects / Q000254:growth & development; D011687:Purines; D013012:Sorbitol / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D058989:Vitrification
chemical_list: D001593:Benzyl Compounds; D003451:Cryoprotective Agents; D011687:Purines; D013012:Sorbitol; D013395:Sucrose; C480551:benzylaminopurine; D007701:Kinetin