title: Enzymatic oxidation of rutin by horseradish peroxidase: kinetic mechanism and identification of a dimeric product by LC-Orbitrap mass spectrometry.
abstract: Flavonoid oxidation is important issue in food processing and quality. The kinetic mechanism of enzymatic oxidation of rutin by horseradish peroxidase (HRP) was studied. Rutin oxidation reaction was followed by recording of spectral changes over the time at 360 nm. The studied oxidation is mostly enzymatic and less part non-enzymatic. The reaction with HRP has a higher rate compared with the reaction without of HRP, whereby is part of non-enzymatic reaction about 10% of the total reaction. Kinetic parameters were determined from graphics of linear Michaelis-Menten equation, and it was found that investigated reactions of rutin oxidation by HRP take place in a ping-pong kinetic mechanism. High resolution HPLC-MS analysis of the mixture of oxidized products of rutin revealed the presence of rutin dimer. Because of widely distribution of rutin as well as presence of peroxidases and hydrogen peroxide in fresh foods identification of this enzymatic modification product can be beneficial for foods quality and safety.
authors.affiliation: Faculty of Technology, University of Nis, Bulevar Oslobodjenja 124, 16000 Leskovac, Serbia.
authors.forename: Sasa
authors.initials: S
authors.lastname: Savic
authors.forename: Katarina
authors.initials: K
authors.lastname: Vojinovic
authors.forename: Sanja
authors.initials: S
authors.lastname: Milenkovic
authors.forename: Andrija
authors.initials: A
authors.lastname: Smelcerovic
authors.forename: Marc
authors.initials: M
authors.lastname: Lamshoeft
authors.forename: Zivomir
authors.initials: Z
authors.lastname: Petronijevic
mesh_terms: D055162:Biocatalysis; D002851:Chromatography, High Pressure Liquid; D019281:Dimerization; D006735:Horseradish Peroxidase / Q000737:chemistry*; D007700:Kinetics; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D012431:Rutin / Q000737:chemistry*
chemical_list: D012431:Rutin; D006735:Horseradish Peroxidase
keywords: Horseradish peroxidase; Kinetic mechanism; Orbitrap; Rutin