title: Nuclear Wave1 is required for reprogramming transcription in oocytes and for normal development.
abstract: Eggs and oocytes have a remarkable ability to induce transcription of sperm after normal fertilization and in somatic nuclei after somatic cell nuclear transfer. This ability of eggs and oocytes is essential for normal development. Nuclear actin and actin-binding proteins have been shown to contribute to transcription, although their mode of action is elusive. Here, we find that Xenopus Wave1, previously characterized as a protein involved in actin cytoskeleton organization, is present in the oocyte nucleus and is required for efficient transcriptional reprogramming. Moreover, Wave1 knockdown in embryos results in abnormal development and defective hox gene activation. Nuclear Wave1 binds by its WHD domain to active transcription components, and this binding contributes to the action of RNA polymerase II. We identify Wave1 as a maternal reprogramming factor that also has a necessary role in gene activation in development.
authors.affiliation: Wellcome Trust/Cancer Research UK Gurdon Institute, The Henry Wellcome Building of Cancer and Developmental Biology, Cambridge, UK. k.miyamoto@gurdon.cam.ac.uk
authors.forename: Kei
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Marta
authors.initials: M
authors.lastname: Teperek
authors.forename: Kosuke
authors.initials: K
authors.lastname: Yusa
authors.forename: George E
authors.initials: GE
authors.lastname: Allen
authors.forename: Charles R
authors.initials: CR
authors.lastname: Bradshaw
authors.forename: J B
authors.initials: JB
authors.lastname: Gurdon
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D065150:Cellular Reprogramming / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D005801:Genes, Homeobox; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology*; D009865:Oocytes / Q000254:growth & development* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012319:RNA Polymerase II / Q000378:metabolism; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D051300:Wiskott-Aldrich Syndrome Protein Family / Q000235:genetics / Q000502:physiology*; D029867:Xenopus Proteins / Q000235:genetics / Q000502:physiology*; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics
chemical_list: D009687:Nuclear Proteins; C583330:WASF1 protein, Xenopus; D051300:Wiskott-Aldrich Syndrome Protein Family; D029867:Xenopus Proteins; D012319:RNA Polymerase II