title: JMJD1C, a JmjC domain-containing protein, is required for long-term maintenance of male germ cells in mice.
abstract: JmjC domain-containing proteins are a class of enzymes responsible for histone demethylation. Previous studies revealed that the JmjC domain-containing protein KDM3A possesses intrinsic demethylase activity toward lysine 9 of histone H3 and plays essential roles in spermiogenesis. In contrast, the biological roles of JMJD1C, a KDM3A homolog in mice, are largely unknown. Here we present the crucial role of JMJD1C in male gametogenesis. Jmjd1c-deficient males became infertile due to the progressive reduction of germ cells after 3 mo of age. Importantly, Jmjd1c-deficient testes frequently contained abnormal tubules lacking developmentally immature germ cells. JMJD1C is most abundantly expressed in undifferentiated spermatogonia in mouse testis. The numbers of ZBTB16-positive spermatogonia and apoptotic germ cells in Jmjd1c-deficient testes decreased and increased in an age-dependent manner, respectively. Our studies demonstrated that JMJD1C contributes to the long-term maintenance of the male germ line.
authors.affiliation: Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, Kyoto, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Kuroki
authors.forename: Mika
authors.initials: M
authors.lastname: Akiyoshi
authors.forename: Mikiyo
authors.initials: M
authors.lastname: Tokura
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Miyachi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nakai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kimura
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Shinkai
authors.forename: Makoto
authors.initials: M
authors.lastname: Tachibana
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D000375:Aging; D017398:Alternative Splicing; D000735:Androstenedione / Q000097:blood; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D015971:Gene Expression Regulation, Enzymologic; D007248:Infertility, Male / Q000097:blood / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D056484:Jumonji Domain-Containing Histone Demethylases / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D050505:Mutant Proteins / Q000378:metabolism; D013091:Spermatogenesis; D013093:Spermatogonia / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D013737:Testis / Q000166:cytology / Q000254:growth & development / Q000378:metabolism / Q000473:pathology; D013739:Testosterone / Q000097:blood
chemical_list: D007527:Isoenzymes; D050505:Mutant Proteins; D013739:Testosterone; D000735:Androstenedione; C554520:JMJD1c protein, mouse; D056484:Jumonji Domain-Containing Histone Demethylases
keywords: aging; epigenetics; histone modifications; male infertility