title: Impact of elevated plasma serotonin on global gene expression of murine megakaryocytes.
abstract: BACKGROUND
  Serotonin (5-HT) is a biogenic amine that also acts as a mitogen and a developmental signal early in rodent embryogenesis. Genetic and pharmacological disruption of 5-HT signaling causes various diseases and disorders via mediating central nervous system, cardiovascular system, and serious abnormalities on a growing embryo. Today, neither the effective modulators on 5-HT signaling pathways nor the genes affected by 5-HT signal are well known yet.
  
  
  RESULTS
  In an attempt to identify the genes altered by 5-HT signaling pathways, we analyzed the global gene expression via the Illumina array platform using the mouse WG-6 v2.0 Expression BeadChip containing 45,281 probe sets representing 30,854 genes in megakaryocytes isolated from mice infused with 5-HT or saline. We identified 723 differentially expressed genes of which 706 were induced and 17 were repressed by elevated plasma 5-HT.
  
  
  CONCLUSIONS
  Hierarchical gene clustering analysis was utilized to represent relations between groups and clusters. Using gene ontology mining tools and canonical pathway analyses, we identified multiple biological pathways that are regulated by 5-HT: (i) cytoskeletal remodeling, (ii) G-protein signaling, (iii) vesicular transport, and (iv) apoptosis and survival. Our data encompass the first extensive genome-wide based profiling in the progenitors of platelets in response to 5-HT elevation in vivo.
authors.affiliation: Biochemistry and Molecular Biology, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas, United States of America.
authors.forename: Charles P
authors.initials: CP
authors.lastname: Mercado
authors.forename: Stephanie
authors.initials: S
authors.lastname: Byrum
authors.forename: Marjorie L
authors.initials: ML
authors.lastname: Beggs
authors.forename: Endrit
authors.initials: E
authors.lastname: Ziu
authors.forename: Preeti
authors.initials: P
authors.lastname: Singh
authors.forename: Vinay R
authors.initials: VR
authors.lastname: Raj
authors.forename: Randy S
authors.initials: RS
authors.lastname: Haun
authors.forename: Fusun
authors.initials: F
authors.lastname: Kilic
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D008533:Megakaryocytes / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D012701:Serotonin / Q000097:blood*
chemical_list: D012701:Serotonin