title: Platelet-rich plasma, low-level laser therapy, or their combination promotes periodontal regeneration in fenestration defects: a preliminary in vivo study.
abstract: BACKGROUND
  This study histomorphometrically analyzes the influence of platelet-rich plasma (PRP), low-level laser therapy (LLLT), or their combination on the healing of periodontal fenestration defects (PFDs) in rats.
  
  
  METHODS
  PFDs were surgically created in the mandibles of 80 rats. The animals were randomly divided into four groups: 1) C (control) and 2) PRP, defects were filled with blood clot or PRP, respectively; 3) LLLT and 4) PRP/LLLT, defects received laser irradiation, were filled with blood clot or PRP, respectively, and then irradiated again. Animals were euthanized at either 10 or 30 days post-surgery. Percentage of new bone (NB), density of newly formed bone (DNB), new cementum (NC), and extension of remaining defect (ERD) were histomorphometrically evaluated. Data were statistically analyzed (analysis of variance; Tukey test, P <0.05).
  
  
  RESULTS
  At 10 days, group PRP presented ERD significantly lower than group C. At 30 days, group PRP presented NB and DNB significantly greater than group C. Groups LLLT, PRP, and PRP/LLLT showed significant NC formation at 30 days, with collagen fibers inserted obliquely or perpendicularly to the root surface. NC formation was not observed in any group C specimen.
  
  
  CONCLUSIONS
  LLLT, PRP, or their combination all promoted NC formation with a functional periodontal ligament. The combination PRP/LLLT did not show additional positive effects compared to the use of either therapy alone.
authors.affiliation: Department of Surgery and Integrated Clinic, Dental School of Araçatuba, UNESP - São Paulo State University, Araçatuba, São Paulo, Brazil.
authors.forename: Maria J H
authors.initials: MJ
authors.lastname: Nagata
authors.forename: Natália
authors.initials: N
authors.lastname: de Campos
authors.forename: Michel R
authors.initials: MR
authors.lastname: Messora
authors.forename: Natália M
authors.initials: NM
authors.lastname: Pola
authors.forename: Carolina S
authors.initials: CS
authors.lastname: Santinoni
authors.forename: Suely R M
authors.initials: SR
authors.lastname: Bomfim
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Fucini
authors.forename: Edilson
authors.initials: E
authors.lastname: Ervolino
authors.forename: Juliano M
authors.initials: JM
authors.lastname: de Almeida
authors.forename: Letícia H
authors.initials: LH
authors.lastname: Theodoro
authors.forename: Valdir G
authors.initials: VG
authors.lastname: Garcia
mesh_terms: D016301:Alveolar Bone Loss / Q000532:radiotherapy / Q000628:therapy*; D000539:Alveolar Process / Q000473:pathology / Q000528:radiation effects; D000818:Animals; D001777:Blood Coagulation / Q000502:physiology; D015519:Bone Density / Q000502:physiology / Q000528:radiation effects; D001861:Bone Regeneration / Q000502:physiology* / Q000528:radiation effects; D003094:Collagen / Q000737:chemistry / Q000528:radiation effects; D003131:Combined Modality Therapy; D003739:Dental Cementum / Q000473:pathology / Q000528:radiation effects; D005347:Fibroblasts / Q000473:pathology / Q000528:radiation effects; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D008336:Mandibular Diseases / Q000532:radiotherapy / Q000628:therapy; D010012:Osteogenesis / Q000502:physiology / Q000528:radiation effects; D053657:Platelet-Rich Plasma / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014092:Tooth Root / Q000473:pathology / Q000528:radiation effects
chemical_list: D003094:Collagen
keywords: Laser therapy; animal; low-level; models; periodontics; platelet-rich plasma; regeneration; wounds and injuries