title: BH3-only proteins Noxa, Bik, Bmf, and Bid activate Bax and Bak indirectly when studied in yeast model.
abstract: BH3-only proteins of the Bcl-2 family regulate programmed cell death in mammals through activation of multidomain proapoptotic proteins Bax and Bak in response to various proapoptotic stimuli by mechanism that remains under dispute. Here, we report that the cell death-promoting activity of BH3-only proteins Bik, Bmf, Noxa, and tBid can only be reconstituted in yeast when both multidomain proapoptotic and antiapoptotic Bcl-2 family proteins are present. Inability of these proteins to induce cell death in the absence of antiapoptotic proteins suggests that all tested BH3-only proteins likely activate Bax and Bak indirectly by inhibiting antiapoptotic proteins.
authors.affiliation: Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Gérecová
authors.forename: Jana
authors.initials: J
authors.lastname: Kopanicová
authors.forename: Petra
authors.initials: P
authors.lastname: Jaká
authors.forename: Lucia
authors.initials: L
authors.lastname: Běhalová
authors.forename: Barbora
authors.initials: B
authors.lastname: Juhásová
authors.forename: Ingrid
authors.initials: I
authors.lastname: Bhatia-Kiššová
authors.forename: Michael
authors.initials: M
authors.lastname: Forte
authors.forename: Peter
authors.initials: P
authors.lastname: Polčic
authors.forename: Marek
authors.initials: M
authors.lastname: Mentel
mesh_terms: D017209:Apoptosis / Q000235:genetics; D051023:BH3 Interacting Domain Death Agonist Protein / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D050998:bcl-2 Homologous Antagonist-Killer Protein / Q000235:genetics / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D051023:BH3 Interacting Domain Death Agonist Protein; D019253:Proto-Oncogene Proteins c-bcl-2; D050998:bcl-2 Homologous Antagonist-Killer Protein; D051028:bcl-2-Associated X Protein
keywords: BH3-only proteins; Bax; Bcl-XL; apoptosis; yeast