title: The combination of mesenchymal stem cells and a bone scaffold in the treatment of vertebral body defects.
abstract: OBJECTIVE
  Vertebral body defects represent one of the most common orthopedic challenges. In order to advance the transfer of stem cell therapies into orthopedic clinical practice, we performed this study to evaluate the safety and efficacy of a composite bioartificial graft based on a hydroxyapatite bone scaffold (CEM-OSTETIC(®)) combined with human mesenchymal stem cells (MSCs) in a rat model of vertebral body defects.
  
  
  METHODS
  Under general isoflurane anesthesia, a defect in the body of the L2 vertebra was prepared and left to heal spontaneously (group 1), implanted with scaffold material alone (group 2), or implanted with a scaffold together with 0.5 million MSCs (group 3) or 5 million MSCs (group 4). The rats were killed 8 weeks after surgery. Histological and histomorphometrical evaluation of the implant as well as micro-CT imaging of the vertebrae were performed.
  
  
  RESULTS
  We observed a significant effect on the formation of new bone tissue in the defect in group 4 when compared to the other groups and a reduced inflammatory reaction in both groups receiving a scaffold and MSCs. We did not detect any substantial pathological changes or tumor formation after graft implantation.
  
  
  CONCLUSIONS
  MSCs in combination with a hydroxyapatite scaffold improved the repair of a model bone defect and might represent a safe and effective alternative in the treatment of vertebral bone defects.
authors.affiliation: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic (ASCR), Vídeňská 1083, 142 20, Prague 4, Czech Republic.
authors.forename: Václav
authors.initials: V
authors.lastname: Vaněček
authors.forename: Karel
authors.initials: K
authors.lastname: Klíma
authors.forename: Aleš
authors.initials: A
authors.lastname: Kohout
authors.forename: René
authors.initials: R
authors.lastname: Foltán
authors.forename: Ondřej
authors.initials: O
authors.lastname: Jiroušek
authors.forename: Jiří
authors.initials: J
authors.lastname: Šedý
authors.forename: Jan
authors.initials: J
authors.lastname: Štulík
authors.forename: Eva
authors.initials: E
authors.lastname: Syková
authors.forename: Pavla
authors.initials: P
authors.lastname: Jendelová
mesh_terms: D000818:Animals; D016025:Bone Transplantation / Q000379:methods*; D004195:Disease Models, Animal; D017886:Durapatite; D006801:Humans; D008159:Lumbar Vertebrae / Q000000981:diagnostic imaging / Q000293:injuries* / Q000473:pathology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D011859:Radiography; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013124:Spinal Injuries / Q000000981:diagnostic imaging / Q000473:pathology / Q000628:therapy*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014945:Wound Healing
chemical_list: D017886:Durapatite