title: Low titer lentiviral transgenesis in rodents with simian immundeficiency virus vector.
abstract: Efficient production of transgenic animals using low-titer lentiviral constructs remains challenging. Here we demonstrate that microinjection of simian immundeficiency virus-derived lentiviral constructs can produce transgenic mice and rats with high efficiency even when using low-titer virus preparations.
authors.affiliation: Institute of Animal Biotechnology, Agricultural Biotechnology Center, Gödöllő, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Bender
authors.forename: Orsolya Ivett
authors.initials: OI
authors.lastname: Hoffmann
authors.forename: Didier
authors.initials: D
authors.lastname: Negre
authors.forename: Krisztián
authors.initials: K
authors.lastname: Kvell
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Bősze
authors.forename: László
authors.initials: L
authors.lastname: Hiripi
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D004622:Embryo, Mammalian; D005260:Female; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells; D051379:Mice; D008846:Micromanipulation; D051381:Rats; D017208:Rats, Wistar; D015302:Simian Immunodeficiency Virus / Q000235:genetics*
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: Lentiviral transgenesis; SIV; low titer; transgenic mice; transgenic rat