title: Analysis of a new histological and molecular-based classification of canine mammary neoplasia.
abstract: Canine mammary tumors (CMTs) are morphologically and biologically heterogeneous, prompting several attempts to classify such tumors on the basis of their histopathological characteristics. Recently, molecular-based analysis methods borrowed from human breast cancer research have also been applied to the classification of CMTs. In this study, canine mammary neoplasms (n = 648) occurring in Korea from 2008 to 2011 were analyzed according to the histological classification and grading system proposed by Goldschmidt et al. Furthermore, randomly selected mammary carcinomas (n = 159) were classified according to the molecular subtype using immunohistochemical characteristics. Canine mammary neoplasia accounted for 52.6% (648/1250) of the tumors in female dogs, and 51.7% (340/648) of these were malignant. All of the carcinoma-anaplastic subtypes were grade III tumors (5/5, 100%), while most of the carcinoma-tubular subtypes (15/18, 83.3%) and carcinoma arising in a complex adenoma/mixed-tumor subtype (115/135, 85.2%) were grade I tumors. Tumor cell invasion into lymphatic vessels was most common in the comedocarcinoma, carcinoma-anaplastic, and inflammatory carcinoma subtypes. The most frequently occurring molecular subtype (70/159, 44%) was luminal A. However, the basal-like subtype was the most malignant and was frequently associated with grade III tumors and lymphatic invasion. The carcinoma-solid subtypes were also often of the basal-like subtype. Reclassification of CMTs using the newly proposed histopathological classification system and molecular subtyping could aid in determining the prognosis and the most suitable anticancer treatment for each case.
authors.affiliation: Department of Veterinary Pathology, Small Animal Tumor Diagnostic Center, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 143-701, Korea. Email: jsur@konkuk.ac.kr.
authors.forename: K S
authors.initials: KS
authors.lastname: Im
authors.forename: N H
authors.initials: NH
authors.lastname: Kim
authors.forename: H Y
authors.initials: HY
authors.lastname: Lim
authors.forename: H W
authors.initials: HW
authors.lastname: Kim
authors.forename: J I
authors.initials: JI
authors.lastname: Shin
authors.forename: J H
authors.initials: JH
authors.lastname: Sur
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002277:Carcinoma / Q000145:classification* / Q000473:pathology / Q000662:veterinary*; D004283:Dog Diseases / Q000145:classification* / Q000453:epidemiology* / Q000473:pathology; D004285:Dogs; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D008207:Lymphatic Metastasis; D015674:Mammary Neoplasms, Animal / Q000145:classification* / Q000453:epidemiology* / Q000473:pathology; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D056910:Republic of Korea / Q000453:epidemiology
keywords: canine mammary neoplasia; histological classification; histological grading; molecular subtype