title: Isolation and characterization of a novel cinosulfuron degrading Kurthia sp. from a methanogenic microbial consortium.
abstract: A novel bacterial strain LAM0713 was isolated from a methanogenic bacterial complexes and identified as Kurthia sp. based on morphological, cultural, physio-biochemical characteristics and analysis of 16S rDNA sequence. Strain LAM0713 was found to be capable of utilizing cinosulfuron as sole nitrogen source for growth over a wide range of temperature (20-40 °C) and pH (5.0-9.0). Response surface methodology was used to optimize the degradation conditions. Strain LAM0713 could efficiently degrade 92.4% of initially supplemented 50 mg·L(1) cinosulfuron under the optimum conditions (pH 6.9, 31.8 °C) within 5 days. Five intermediates formed during cinosulfuron degradation were detected by liquid chromatography mass spectrometry (LC-MS), and a metabolic pathway for cinosulfuron degradation was proposed via cleavage of the sulfonylurea bridge. It is the first report showing that Kurthia sp. strain could degrade sulfonylurea herbicides, suggesting that strain LAM0713 may provide new insight into microbial degradation of herbicides.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Microbial Resources Collection and Preservation, Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Ruan
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Civil and Environmental Engineering, Stanford University, Stanford, CA 94305, USA.
authors.forename: Shenghua
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin 150000, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.affiliation: Institute of Agriculture Engineering, Chongqing Academy of Agricultural Sciences, Chongqing 401329, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhai
authors.affiliation: Key Laboratory of Microbial Resources Collection and Preservation, Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Microbial Resources Collection and Preservation, Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: binzhao@mail.hzau.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
mesh_terms: D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D006540:Herbicides / Q000378:metabolism*; D013058:Mass Spectrometry; D059013:Microbial Consortia; D013453:Sulfonylurea Compounds / Q000378:metabolism*; D000096926:Benzenesulfonamides
chemical_list: D006540:Herbicides; D013453:Sulfonylurea Compounds; C469574:cinosulfuron; D000096926:Benzenesulfonamides
keywords: Biodegradation; Cinosulfuron; Kurthia sp.; Novel species