title: Avian-specific real-time PCR assay for authenticity control in farm animal feeds and pet foods.
abstract: A highly sensitive TaqMan real-time PCR assay targeting the mitochondrial 12S rRNA gene was developed for detection of an avian-specific DNA fragment (68bp) in farm animal and pet feeds. The specificity of the assay was verified against a wide representation of animal and plant species. Applicability assessment of the avian real-time PCR was conducted through representative analysis of two types of compound feeds: industrial farm animal feeds (n=60) subjected to extreme temperatures, and commercial dog and cat feeds (n=210). Results obtained demonstrated the suitability of the real-time PCR assay to detect the presence of low percentages of highly processed avian material in the feed samples analysed. Although quantification results were well reproducible under the experimental conditions tested, an accurate estimation of the target content in feeds is impossible in practice. Nevertheless, the method may be useful as an alternative tool for traceability purposes within the framework of feed control.
authors.affiliation: Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain. npegels@vet.ucm.es
authors.forename: Nicolette
authors.initials: N
authors.lastname: Pegels
authors.forename: Isabel
authors.initials: I
authors.lastname: González
authors.forename: Teresa
authors.initials: T
authors.lastname: García
authors.forename: Rosario
authors.initials: R
authors.lastname: Martín
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D030161:Avian Proteins / Q000032:analysis / Q000235:genetics*; D001717:Birds / Q000235:genetics; D005399:Fishes / Q000235:genetics; D005506:Food Contamination / Q000032:analysis*; D008322:Mammals / Q000235:genetics; D057805:Pets / Q000378:metabolism; D010944:Plants / Q000235:genetics; D011786:Quality Control; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D030161:Avian Proteins
keywords: 12S rRNA gene; Avian; Farm animal feeds; Pet feeds; TaqMan real-time PCR; Traceability