title: Molecular and biological properties of tomato necrotic stunt virus and development of a sensitive real-time RT-PCR assay.
abstract: The newly identified tomato necrotic stunt virus (ToNStV), with a genome of 10,057 nucleotides, had 60 % nucleotide sequence and 70 % coat protein amino acid sequence identity to other potyviruses. Analysis of a second ToNStV isolate revealed two single nucleotide substitutions. ToNStV induced stunting and chlorotic to necrotic leaf symptoms on tomato plants. In host-range assays, ToNStV systemically infected many species in the family Solanaceae and locally infected some species in the families Amaranthaceae, Brassicaceae and Cucurbitaceae. A sensitive and reliable real-time RT-PCR assay was developed using simply prepared crude tissue extract.
authors.affiliation: U.S. Vegetable Laboratory, USDA-Agricultural Research Service, 2700 Savannah Highway, Charleston, SC, 29414, USA, Rugang.Li@ars.usda.gov.
authors.forename: Rugang
authors.initials: R
authors.lastname: Li
authors.forename: Zhangjun
authors.initials: Z
authors.lastname: Fei
authors.forename: Kai-Shu
authors.initials: KS
authors.lastname: Ling
mesh_terms: D016000:Cluster Analysis; D058507:Host Specificity; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D020641:Polymorphism, Single Nucleotide; D017800:Potyvirus / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012367:RNA, Viral / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014773:Virology / Q000379:methods*
chemical_list: D012367:RNA, Viral