title: [Quercetin regulates cell cycle-related gene expression in a model of glucose-oxygen deprivation in astrocytes].
abstract: OBJECTIVE
  To study the effect of quercetin on gene expression in astrocytes after glucose-oxygen deprivation and the underlying mechanism.
  
  
  METHODS
  The primary cultured astrocytes were randomly divided into glucose-oxygen deprivation group (only treated with glucose-oxygen deprivation for 4 hours) and glucose-oxygen deprivation combined with quercetin-treated group (glucose-oxygen deprivation for 4 hours combined with quercetin treatment for 24 hours). Their mRNA expressions were analyzed by the large-scale oligo microarray. The differential genes obtained were further confirmed by real-time quantitative PCR (qRT-PCR).
  
  
  RESULTS
  Compared with the glucose-oxygen deprivation group, the glucose-oxygen deprivation combined with quercetin-treated group presented the changes in the expressions of 31 genes that were related to cell cycle, of which 5 genes were up-regulated and 26 were down-regulated. Six of those differential genes were confirmed by qRT-PCR and the result of their differential expressions was consistent with that by large-scale oligo microarray.
  
  
  CONCLUSIONS
  Quercetin can regulate some of cell cycle-related genes in astrocytes after glucose-oxygen deprivation.
authors.affiliation: Research Center of Clinical Medicine, Southern Medical University, Guangzhou, China. fangfang5087@126.com
authors.forename: Fang
authors.initials: F
authors.lastname: Yao
authors.forename: Lanlan
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhaohu
authors.initials: Z
authors.lastname: Yuan
authors.forename: Yong
authors.initials: Y
authors.lastname: Zeng
authors.forename: Bingyi
authors.initials: B
authors.lastname: Wu
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D002453:Cell Cycle; D015687:Cell Hypoxia; D002478:Cells, Cultured; D051357:Cyclin-Dependent Kinase 2 / Q000235:genetics; D016213:Cyclins / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D005947:Glucose; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D016213:Cyclins; D011794:Quercetin; D051357:Cyclin-Dependent Kinase 2; D005947:Glucose