title: Anionic polymers and 10 nm Fe₃O₄@UA wound dressings support human foetal stem cells normal development and exhibit great antimicrobial properties.
abstract: The aims of this study were the development, characterization and bioevaluation of a novel biocompatible, resorbable and bio-active wound dressing prototype, based on anionic polymers (sodium alginate--AlgNa, carboximethylcellulose--CMC) and magnetic nanoparticles loaded with usnic acid (Fe₃O₄@UA). The antimicrobial activity was tested against Staphylococcus aureus grown in biofilms. The biocompatibility testing model included an endothelial cell line from human umbilical vein and human foetal progenitor cells derived from the amniotic fluid, that express a wide spectrum of surface molecules involved in different vascular functions and inflammatory response, and may be used as skin regenerative support. The obtained results demonstrated that CMC/Fe₃O₄@UA and AlgNa/Fe₃O₄@UA are exhibiting structural and functional properties that recommend them for further applications in the biomedical field. They could be used alone or coated with different bio-active compounds, such as Fe₃O₄@UA, for the development of novel, multifunctional porous materials used in tissues regeneration, as antimicrobial substances releasing devices, providing also a mechanical support for the eukaryotic cells adhesion, and exhibiting the advantage of low cytotoxicity on human progenitor cells. The great antimicrobial properties exhibited by the newly synthesized nano-bioactive coatings are recommending them as successful candidates for improving the implanted devices surfaces used in regenerative medicine.
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Department of Science and Engineering of Oxide Materials and Nanomaterials, Polizu Street No. 1-7, 011061 Bucharest, Romania.
authors.forename: Alexandru Mihai
authors.initials: AM
authors.lastname: Grumezescu
authors.affiliation: University of Bucharest, Faculty of Biology, Microbiology Department, Aleea Portocalelor, No. 1-3, 060101 Bucharest, Romania. Electronic address: alina_m_h@yahoo.com.
authors.forename: Alina Maria
authors.initials: AM
authors.lastname: Holban
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Department of Science and Engineering of Oxide Materials and Nanomaterials, Polizu Street No. 1-7, 011061 Bucharest, Romania.
authors.forename: Ecaterina
authors.initials: E
authors.lastname: Andronescu
authors.affiliation: Department of Pharmacognosy & Phytotherapy, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, 2 Petru Rareş Street, 200349 Craiova, Romania.
authors.forename: George Dan
authors.initials: GD
authors.lastname: Mogoşanu
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Department of Science and Engineering of Oxide Materials and Nanomaterials, Polizu Street No. 1-7, 011061 Bucharest, Romania.
authors.forename: Bogdan Stefan
authors.initials: BS
authors.lastname: Vasile
authors.affiliation: University of Bucharest, Faculty of Biology, Microbiology Department, Aleea Portocalelor, No. 1-3, 060101 Bucharest, Romania.
authors.forename: Mariana Carmen
authors.initials: MC
authors.lastname: Chifiriuc
authors.affiliation: University of Bucharest, Faculty of Biology, Microbiology Department, Aleea Portocalelor, No. 1-3, 060101 Bucharest, Romania.
authors.forename: Veronica
authors.initials: V
authors.lastname: Lazar
authors.affiliation: Flow Cytometry and Cell Therapy Laboratory, Institute of Cellular Biology and Pathology "Nicolae Simionescu" (ICBP), Bucharest, Romania.
authors.forename: Eugen
authors.initials: E
authors.lastname: Andrei
authors.affiliation: Flow Cytometry and Cell Therapy Laboratory, Institute of Cellular Biology and Pathology "Nicolae Simionescu" (ICBP), Bucharest, Romania.
authors.forename: Andrei
authors.initials: A
authors.lastname: Constantinescu
authors.affiliation: Flow Cytometry and Cell Therapy Laboratory, Institute of Cellular Biology and Pathology "Nicolae Simionescu" (ICBP), Bucharest, Romania.
authors.forename: Horia
authors.initials: H
authors.lastname: Maniu
mesh_terms: D000464:Alginates / Q000008:administration & dosage* / Q000737:chemistry; D000890:Anti-Infective Agents / Q000008:administration & dosage* / Q000737:chemistry; D001458:Bandages; D001572:Benzofurans / Q000008:administration & dosage* / Q000737:chemistry; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D002266:Carboxymethylcellulose Sodium / Q000008:administration & dosage* / Q000737:chemistry; D002460:Cell Line; D002478:Cells, Cultured; D053686:Fetal Stem Cells / Q000502:physiology; D020723:Glucuronic Acid / Q000008:administration & dosage / Q000737:chemistry; D006603:Hexuronic Acids / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D058185:Magnetite Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology; D014961:X-Ray Diffraction
chemical_list: D000464:Alginates; D000890:Anti-Infective Agents; D001572:Benzofurans; D006603:Hexuronic Acids; D058185:Magnetite Nanoparticles; C073339:usnic acid; D020723:Glucuronic Acid; D002266:Carboxymethylcellulose Sodium
keywords: Carboximethylcellulose; Human foetal progenitor cells; Magnetite nanoparticles; Regenerative medicine; Sodium alginate; Wound dressing