title: A novel high throughput method based on the DPPH dry reagent array for determination of antioxidant activity.
abstract: A stable chromogenic radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) is commonly used for the determination of antioxidant activity. In this paper, DPPH was dried into 96 well microplate to produce DPPH dry reagent array plate, based on which the highly sensitive and high throughput determination of antioxidant activities was achieved. The spectrophotometric characterization of the microplate containing dried or fresh DPPH free radicals was reported. The response of the DPPH dry reagent array towards different standard antioxidants was studied. The reaction for DPPH in fresh or dry reagent array with Trolox was reported and compared. The DPPH dry reagent array was used to study the antioxidant activity of banana, green tea, pink guava, and honeydew and the results were compared to the samples reacted with freshly prepared DPPH. The proposed method is comparable to the classical DPPH method, more convenient, simple to operate with minimal solvent required and excellent sensitivity.
authors.affiliation: School of Chemical Sciences & Food Technology, Faculty of Science & Technology, 43600 UKM, Bangi Selangor, Malaysia. khalid.hamid@ukm.my
authors.forename: Khalid Hamid
authors.initials: KH
authors.lastname: Musa
authors.forename: Aminah
authors.initials: A
authors.lastname: Abdullah
authors.forename: Bambang
authors.initials: B
authors.lastname: Kuswandi
authors.forename: M Amrun
authors.initials: MA
authors.lastname: Hidayat
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D001628:Beverages / Q000032:analysis*; D001713:Biphenyl Compounds / Q000737:chemistry*; D028241:Camellia sinensis / Q000737:chemistry*; D057166:High-Throughput Screening Assays / Q000379:methods*; D028521:Musa / Q000737:chemistry*; D010084:Oxidation-Reduction; D010851:Picrates / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D031647:Psidium / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: 96 well microplate; Antioxidant activity; DPPH free radicals; Dry reagent array