title: Efficacy of dinotefuran, permethrin and pyriproxyfen combination spot-on on dogs against Phlebotomus perniciosus and Ctenocephalides canis.
abstract: This study was conducted to evaluate the efficacy of a new topical ectoparasiticidal spot-on containing 4.95% dinotefuran (w/w), 36.08% permethrin (w/w) and 0.44% pyriproxyfen (w/w) (Vectra 3D, Ceva, Libourne, France) against Portuguese strain of Phlebotomus perniciosus and a French strain of Ctenocephalides canis in dogs. Twelve beagle dogs were exposed for 1 h to 100 P. perniciosus on day 6 for allocation in two groups. One group was treated on day 0, and the other group was the control group. The dogs were exposed for 1 h to 100 P. perniciosus on days 1, 7, 14, 21 and 28. After each sandfly challenge, the same dogs were infested with 100 C. canis. Counts of living fleas were determined 48 h after infestation on days 4, 3, 9, 16, 23 and 30. For sandflies, the anti-feeding effect was 96.9, 99.7, 98.7, 83.5 and 87.0 % on days 1, 7, 14, 21 and 28, respectively. The mortality effect was 97.8, 99.8, 73.7, 27.5 and 39.6% on days 1, 7, 14, 21 and 28, respectively. At each challenge point, the mortality and anti-feeding effects on sandflies were significantly different between the control and treatment groups (p < 0.05). The adulticidal effect on C. canis remained above 99% throughout the study period. The results indicate that a combination with dinotefuran, permethrin and pyriproxyfen may be used as an effective part of an overall flea and sandfly control strategy in dogs for monthly use.
authors.affiliation: Laboratory of Parasitology and Parasitic Diseases, INP-ENVT, National Veterinary School of Toulouse, 31076, Toulouse, France.
authors.forename: E
authors.initials: E
authors.lastname: Liénard
authors.forename: E
authors.initials: E
authors.lastname: Bouhsira
authors.forename: P
authors.initials: P
authors.lastname: Jacquiet
authors.forename: S
authors.initials: S
authors.lastname: Warin
authors.forename: V
authors.initials: V
authors.lastname: Kaltsatos
authors.forename: M
authors.initials: M
authors.lastname: Franc
mesh_terms: D000818:Animals; D058251:Ctenocephalides / Q000187:drug effects; D004283:Dog Diseases / Q000188:drug therapy*; D004285:Dogs; D004359:Drug Therapy, Combination / Q000379:methods; D058267:Flea Infestations / Q000188:drug therapy / Q000662:veterinary*; D006146:Guanidines / Q000627:therapeutic use*; D007306:Insecticides / Q000627:therapeutic use*; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000627:therapeutic use*; D059208:Parasite Load; D026023:Permethrin / Q000627:therapeutic use*; D010691:Phlebotomus / Q000187:drug effects; D010217:Phlebotomus Fever / Q000188:drug therapy / Q000662:veterinary*; D011725:Pyridines / Q000627:therapeutic use*; D016019:Survival Analysis; D016896:Treatment Outcome
chemical_list: D006146:Guanidines; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; D011725:Pyridines; C465368:dinotefuran; C055613:pyriproxyfen; D026023:Permethrin