title: Phenotypic characterization of GPR120-expressing cells in the interstitial tissue of pancreas.
abstract: GPR120 functions as a plasma membrane receptor for unsaturated long-chain free fatty acids and involves in GLP-1 secretion, adipogenesis and the control of energy balance. Pancreas is the key organ in fuel and energy metabolism. Here GPR120 expression in human and rat pancreas was observed by RT-PCR, and the distribution and phenotypes of GPR120-positive cells in human and rat pancreas were shown by immunohistochemical staining. GPR120 mRNA expression was found in human and rat pancreas. GPR120-positive cells were scattered mainly in the interstitial tissues of human and rat pancreas, and they were not co-localized with nestin, vimentin, alpha-SMA and glucagon, respectively. However, GPR120 was distributed on the cells positively stained by CD68, the specific marker of macrophages, and on the cells positive stained by CD34 and CD117, the markers of interstitial cells. In conclusion, this study demonstrates the expression of GPR120 in pancreas and shows the distribution of GPR120 in human and rat pancreas.
authors.affiliation: Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Zhao
authors.forename: Dingjun
authors.initials: D
authors.lastname: Zha
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Qiao
authors.forename: Lianjun
authors.initials: L
authors.lastname: Lu
authors.forename: Lin
authors.initials: L
authors.lastname: Mei
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Jianhua
authors.initials: J
authors.lastname: Qiu
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics; D002460:Cell Line; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005786:Gene Expression Regulation; D005934:Glucagon / Q000235:genetics; D052216:Glucagon-Like Peptide 1 / Q000096:biosynthesis / Q000378:metabolism*; D006801:Humans; D008264:Macrophages / Q000378:metabolism*; D010179:Pancreas / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis* / Q000235:genetics; D014018:Tissue Distribution; D014746:Vimentin / Q000235:genetics; D000097382:CD68 Molecule
chemical_list: D000199:Actins; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D005231:Fatty Acids, Unsaturated; D005934:Glucagon; D052216:Glucagon-Like Peptide 1; D043562:Receptors, G-Protein-Coupled; D014746:Vimentin; C541116:ACTA2 protein, human; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C533284:Ffar4 protein, rat
keywords: CD117; CD34; CD68; GPR120; Pancreas