title: Presenting a foreign antigen on live attenuated Edwardsiella tarda using twin-arginine translocation signal peptide as a multivalent vaccine.
abstract: The twin-arginine translocation (Tat) system is a major pathway for transmembrane translocation of fully folded proteins. In this study, a multivalent vaccine to present foreign antigens on live attenuated vaccine Edwardsiella tarda WED using screened Tat signal peptide was constructed. Because the Tat system increases the yields of folded antigens in periplasmic space or extracellular milieu, it is expected to contribute to the production of conformational epitope-derived specific antibodies. E. tarda Tat signal peptides fused with the green fluorescent protein (GFP) was constructed under the control of an in vivo inducible dps promoter. The resulting plasmids were electroporated into WED and the subcellular localizations of GFP were analyzed with Western blotting. Eight signal peptides with optimized GFP translocation efficiency were further fused to a protective antigen glyceraldehyde-3-phosphate dehydrogenase (GapA) from a fish pathogen Aeromonas hydrophila. Signal peptides of DmsA, NapA, and SufI displayed high efficiency for GapA translocation. The relative percent survival (RPS) of turbot was measured with a co-infection of E. tarda and A. hydrophila, and the strain with DmsA signal peptide showed the maximal protection. This study demonstrated a new platform to construct multivalent vaccines using optimized Tat signal peptide in E. tarda.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Yamin
authors.initials: Y
authors.lastname: Wang
authors.forename: Weizheng
authors.initials: W
authors.lastname: Yang
authors.forename: Qiyao
authors.initials: Q
authors.lastname: Wang
authors.forename: Jiangbo
authors.initials: J
authors.lastname: Qu
authors.forename: Yuanxing
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D016980:Aeromonas hydrophila / Q000201:enzymology; D000818:Animals; D017951:Antigen Presentation / Q000235:genetics*; D020609:Edwardsiella tarda / Q000235:genetics / Q000378:metabolism; D004756:Enterobacteriaceae Infections / Q000517:prevention & control; D029968:Escherichia coli Proteins / Q000235:genetics / Q000276:immunology*; D005399:Fishes / Q000382:microbiology*; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000235:genetics / Q000276:immunology; D026901:Membrane Transport Proteins / Q000235:genetics / Q000276:immunology*; D011401:Promoter Regions, Genetic; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology; D022223:Vaccines, Subunit / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology
chemical_list: D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D014613:Vaccines, Attenuated; D022223:Vaccines, Subunit; C408585:twin-arginine translocase complex, E coli; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)
keywords: Aeromonas hydrophila; Edwardsiella tarda; Multivalent vaccine; Signal peptide; Twin-arginine translocation system