title: Exopolysaccharide from Lactobacillus planterum LP6: antioxidation and the effect on oxidative stress.
abstract: An exopolysaccharide (EPS-3) was isolated from the culture of Lactobacillus planterum LP6 and purified by ion exchange and gel chromatography. The concentrations required to scavenge 50% of the initial radical for DPPH·, OH· and O2(·-) radicals were 1.38, 3.43 and 0.11 mg/mL, respectively. The reducing power (A700 nm) was 0.632 at 5mg/mL. The cell viability of PC12 was improved by 21.67% at 200 μg/mL of EPS-3. Compared with the H2O2 group, the total-antioxidant capacity, activities of superoxide dismutase and catalase were enhanced by 65.81%, 41.34% and 59.05%, respectively. Meanwhile, the level of malondialdehyde and lactate dehydrogenase were decreased by 52.80% and 30.24%. The result indicated that EPS-3 had a notable protective effect against oxidative damage on PC12 cells. The study might lay a theoretical foundation for the comprehensive utilization of lactic acid bacteria source which could result in its application in food systems.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, PR China.
authors.forename: Jing-Yan
authors.initials: JY
authors.lastname: Li
authors.forename: Man-Man
authors.initials: MM
authors.lastname: Jin
authors.forename: Jun
authors.initials: J
authors.lastname: Meng
authors.forename: Shu-Ming
authors.initials: SM
authors.lastname: Gao
authors.forename: Rong-Rong
authors.initials: RR
authors.lastname: Lu
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000737:chemistry*; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D016166:Free Radical Scavengers; D011135:Polysaccharides, Bacterial; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Antioxidant activity; Exopolysaccharide; Isolation and purification; Lactic acid bacteria; Rat pheochromocytoma line 12