title: Comparison of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay with conventional methods for detection of IMP-6, VIM-2, NDM-1, SIM-1, KPC-1, OXA-23, and OXA-51 carbapenemase-producing Acinetobacter spp., Pseudomonas aeruginosa, and Klebsiella pneumoniae.
abstract: Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay was able to detect carbapenemase producers, including SIM-1 or OXA-51, within 4 hours using 20 μL of 0.5 g/L ertapenem solution as a substrate. This assay is more rapid and accurate than the modified Hodge test and 3-dimensional extract bioassay. Hence, it can be used an alternative test to identify carbapenemase-mediated carbapenem resistance in Gram-negative bacteria.
authors.affiliation: Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Korea; Department of Laboratory Medicine, Keimyung University School of Medicine, Daegu 700-712, Korea.
authors.forename: Wonmok
authors.initials: W
authors.lastname: Lee
authors.forename: Hae-Sun
authors.initials: HS
authors.lastname: Chung
authors.forename: Yangsoon
authors.initials: Y
authors.lastname: Lee
authors.forename: Dongeun
authors.initials: D
authors.lastname: Yong
authors.forename: Seok Hoon
authors.initials: SH
authors.lastname: Jeong
authors.forename: Kyungwon
authors.initials: K
authors.lastname: Lee
authors.forename: Yunsop
authors.initials: Y
authors.lastname: Chong
mesh_terms: D000150:Acinetobacter / Q000201:enzymology*; D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D001618:beta-Lactamases
keywords: Acinetobacter spp; Carbapenemase; MALDI-TOF MS; OXA; SIM-1