title: Relative effect of different inorganic acids on selective enrichment of acidogenic biocatalyst for fermentative biohydrogen production from wastewater.
abstract: The effect of different inorganic acids viz., HNO3, HCl, H2SO4 and H3PO4 on inoculum pretreatment to selectively enrich hydrogen (H2) producing acidogenic bacteria was evaluated in anaerobic sequencing batch bioreactors. Relative positive efficiency of HNO3 pretreated consortia in enhancing H2 production (11.85 mol H2/kg CODR) was noticed compared to other acids (HCl, 5.64 mol H2/kg CODR; H2SO4, 7.65 mol H2/kg CODR; H3PO4, 6.90 mol H2/kg CODR) and untreated-parent consortia (control, 6.80 mol H2/kg CODR). On the contrary, substrate degradation (COD removal) was higher with the control operation (ξCOD, 66.3%; substrate degradation rate (SDR), 1.42 kg CODR/m(3)-day) compared to pre-treated culture. HNO3 pre-treatment resulted in a shift in the fermentation pathway towards more acetic acid production, while other acid pretreatment and untreated culture showed mixed type fermentation (acetic, butyric, propionic acids). The bio-electrochemical analysis and dehydrogenase activity supported the biocatalyst performance after HNO3 pretreatment with specific enrichment of Firmicutes and Bacillus.
authors.affiliation: Bioengineering and Environmental Centre (BEEC), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India.
authors.forename: Omprakash
authors.initials: O
authors.lastname: Sarkar
authors.affiliation: Bioengineering and Environmental Centre (BEEC), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India.
authors.forename: R
authors.initials: R
authors.lastname: Kannaiah Goud
authors.affiliation: Bioengineering and Environmental Centre (BEEC), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India.
authors.forename: G
authors.initials: G
authors.lastname: Venkata Subhash
authors.affiliation: Bioengineering and Environmental Centre (BEEC), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India. Electronic address: vmohan_s@yahoo.com.
authors.forename: S
authors.initials: S
authors.lastname: Venkata Mohan
mesh_terms: D000693:Anaerobiosis; D001419:Bacteria / Q000378:metabolism; D055162:Biocatalysis; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D017404:In Situ Hybridization, Fluorescence; D012722:Sewage; D062065:Wastewater / Q000737:chemistry*
chemical_list: D006859:Hydrogen; D012722:Sewage; D062065:Wastewater
keywords: Anaerobic consortia; Anaerobic sequencing reactors (AnSBR); Dehydrogenase activity; FISH; Pretreatment