title: Comparison of primary and secondary metabolites for suitability to discriminate the origins of Schisandra chinensis by GC/MS and LC/MS.
abstract: Discrimination of the origins of plants as traditional medicinal herbs or functional foods is important to accurately comprehend their therapeutic effects or to appropriately utilize their qualities because different environmental backgrounds can induce diverse metabolic changes. In the present study, the origins of the herbal medicine Schisandra chinensis were differentiated using two instrumental approaches, GC/MS and LC/MS. The acquired data were processed using various programs to detect metabolites and statistically examined to measure the suitability of the methods. The R(2)X value of the PCA analysis was used to examine the identified metabolites as potential discriminative markers. The identification of markers by primary metabolites using GC/MS analysis was advantageous because of its reproducibility and the use of a constructed database. However, LC/MS analysis using secondary metabolites provided a greater number of distinguishable variables and higher qualitative R(2)X values for the markers, which suggested that determination of the origins of the plants was more favourable using secondary metabolites.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Dong-Kyu
authors.initials: DK
authors.lastname: Lee
authors.forename: Min Hye
authors.initials: MH
authors.lastname: Yoon
authors.forename: Yun Pyo
authors.initials: YP
authors.lastname: Kang
authors.forename: Jin
authors.initials: J
authors.lastname: Yu
authors.forename: Jeong Hill
authors.initials: JH
authors.lastname: Park
authors.forename: Jeongmi
authors.initials: J
authors.lastname: Lee
authors.forename: Sung Won
authors.initials: SW
authors.lastname: Kwon
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016002:Discriminant Analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005843:Geography; D013058:Mass Spectrometry / Q000379:methods*; D032141:Schisandra / Q000737:chemistry* / Q000378:metabolism; D064210:Secondary Metabolism
keywords: GC/MS; LC/MS; Metabolomics; Primary metabolites; Schisandra chinensis; Secondary metabolites