title: Molecular cloning.
abstract: This protocol describes the basic steps involved in conventional plasmid-based cloning. The goals are to insert a DNA fragment of interest into a receiving vector plasmid, transform the plasmid into E. coli, recover the plasmid DNA, and check for correct insertion events.
authors.affiliation: Department of Biochemistry and Molecular Biology, Johns Hopkins School of Public Health, Baltimore, MD, USA. Electronic address: jkellne2@jhmi.edu.
authors.forename: Juliane C
authors.initials: JC
authors.lastname: Lessard
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D004926:Escherichia coli; D005822:Genetic Vectors; D010957:Plasmids; D016133:Polymerase Chain Reaction
chemical_list: D004247:DNA
keywords: E. coli; Ligation; Molecular cloning; Primer design; Successful ligation events