title: Purification, structure, lipid lowering and liver protecting effects of polysaccharide from Lachnum YM281.
abstract: The extracellular polysaccharide (LEP) produced by Lachnum YM281 was obtained from the fermentation broth, and LEP-1b with molecular weight of 4.02×10(4) Da was separated and sequentially purified through DEAE-cellulose 52 column chromatography and Sepharose CL-6B column chromatography. GC-MS, IR and NMR ((1)H, (13)C) spectroscopy analysis indicated that the repeat unit of LEP-1b was: [formula; see text] The effects of LEP-1b on the serum lipids, liver lipids levels and aminotransferase activities of model mice with hyperlipidemic fatty live were studied, and the results showed that LEP-1b had strong lipid lowering and liver protecting effects on mice with hyperlipidemic fatty live.
authors.affiliation: Microbial Resources and Application Laboratory, College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Qiu
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Ma
authors.forename: Ming
authors.initials: M
authors.lastname: Ye
authors.forename: Ruyue
authors.initials: R
authors.lastname: Yuan
authors.forename: Yanna
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000737:chemistry* / Q000166:cytology; D002240:Carbohydrate Sequence; D019610:Cytoprotection / Q000187:drug effects*; D004435:Eating / Q000187:drug effects; D005110:Extracellular Space / Q000737:chemistry; D005260:Female; D000960:Hypolipidemic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects; D008055:Lipids / Q000097:blood; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000960:Hypolipidemic Agents; D008055:Lipids; D011134:Polysaccharides
keywords: Extracellular polysaccharide; Lachnum YM281; Lipid lowering; Liver protecting; Structure