title: Mesenchymal stromal cells versus betamethasone can dampen disease activity in the collagen arthritis mouse model.
abstract: The objective of this study was to compare between the effects of mesenchymal stem cell (MSC) and betamethasone in the treatment of rheumatoid arthritis. Sixty male albino mice were divided equally into 2 models. They are MSC model, group 1: saline control group, group 2: collagen-induced arthritis (CIA), group 3: induced arthritis mice that received intravenous injection of MSCs. Betamethasone model, group 1: phosphate buffer saline, group 2: CIA, group 3: induced arthritis mice that received intraperitoneal injection of betamethasone. Mice arthritis models were assessed by clinical paw edema and X-rays, at the proper time of sacrefaction, tissues were collected and examined using real-time PCR, and synovial tissue was examined for interleukin-10, tumor necrosis factor α, cartilage oligomeric matrix protein and matrix metalloproteinase 3. While serum levels of rheumatoid factor and C-reactive protein were detected by enzyme-linked immunosorbent assay kits. Also blood erythrocyte sedimentation rate was detected. Histopathological, paw edema and PCR results showed improvement in the groups that received MSC compared with the diseased group and the groups which received betamethasone. MSC significantly enhanced the effect of collagen-induced arthritis treatment, which is superior to betamethasone treatment, likely through the modulation of the expression of various cytokines.
authors.affiliation: Faculty of Pharmacy, Cairo University, Cairo, Egypt.
authors.forename: E S M
authors.initials: ES
authors.lastname: El-Denshary
authors.forename: L A
authors.initials: LA
authors.lastname: Rashed
authors.forename: M
authors.initials: M
authors.lastname: Elhussiny
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D001169:Arthritis, Experimental / Q000473:pathology* / Q000628:therapy*; D001623:Betamethasone / Q000008:administration & dosage*; D001799:Blood Sedimentation; D002356:Cartilage / Q000473:pathology; D004195:Disease Models, Animal; D005121:Extremities / Q000000981:diagnostic imaging / Q000473:pathology; D020869:Gene Expression Profiling; D006651:Histocytochemistry; D016753:Interleukin-10 / Q000032:analysis; D008297:Male; D064235:Matrilin Proteins / Q000032:analysis; D019278:Matrix Metalloproteinase 3 / Q000032:analysis; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D011859:Radiography; D013582:Synovial Fluid; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D000893:Anti-Inflammatory Agents; D064235:Matrilin Proteins; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D001623:Betamethasone; D019278:Matrix Metalloproteinase 3