title: A spectrophotometric flow injection system for streptomycin determination in veterinary samples.
abstract: In this work a spectrophotometric flow injection analysis system for streptomycin determination in veterinary samples, is being proposed. The method is based on streptomycin alkaline hydrolysis that forms guanidine, followed by the reaction with Fe(II). The colored product has absorption peak at 520 nm. To evaluate and optimize the system parameters, chemometrics tools, such as factorial design, Pareto chart and Doelhert design, were used. The veterinary samples are diluted in water and introduced in the FIA system, therefore no sample preparation is required. The optimized system presented: linear range of 60 up to 1000 mg L(-1), limit of detection of 18 mg L(-1) and sampling rate of 36 readings per hour. The precision was checked and the CV for veterinary sample readings were always less than 6.5%. The accuracy was studied by comparison with chromatographic method, thus, five samples of pharmaceutical veterinary were determined by HPLC and by the proposed method, and the results are in agreement (t-test, p=0.05).
authors.affiliation: Universidade Federal de Alfenas (UNIFAL-MG), Instituto de Química, Rua Gabriel Monteiro da Silva, 714, CEP 37130-000 Alfenas, MG, Brazil.
authors.forename: Pedro Marcos
authors.initials: PM
authors.lastname: Frugeri
authors.forename: Ayla Campos
authors.initials: AC
authors.lastname: do Lago
authors.forename: Célio
authors.initials: C
authors.lastname: Wisniewski
authors.forename: Pedro Orival
authors.initials: PO
authors.lastname: Luccas
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005296:Ferrous Compounds / Q000737:chemistry; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000662:veterinary*; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation / Q000662:veterinary*; D013307:Streptomycin / Q000032:analysis*
chemical_list: D005296:Ferrous Compounds; D013307:Streptomycin
keywords: Doelhert design; FIA system; Streptomycin