title: High sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles.
abstract: Improved detection of anti-carbohydrate antibodies is a need in clinical identification of biomarkers for cancer cells or pathogens. Here, we report a new ELISA approach for the detection of specific immunoglobulins (IgGs) against carbohydrates. Two nanometer gold glyconanoparticles bearing oligosaccharide epitopes of HIV or Streptococcus pneumoniae were used as antigens to coat ELISA-plates. A ~3,000-fold improved detection of specific IgGs in mice immunized against S. pneumoniae respect to the well known BSA-glycoconjugate ELISA was achieved. Moreover, these multivalent glyconanoparticles have been employed in solid phase assays to detect the carbohydrate-dependent binding of human dendritic cells and the lectin DC-SIGN. Multivalent glyconanoparticles in ELISA provide a versatile, easy and highly sensitive method to detect and quantify the binding of glycan to proteins and to facilitate the identification of biomarkers.
authors.affiliation: Laboratory of GlycoNanotechnology, Biofunctional Nanomaterials Unit, CIC biomaGUNE, San Sebastián, Spain.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Chiodo
authors.forename: Marco
authors.initials: M
authors.lastname: Marradi
authors.forename: Boris
authors.initials: B
authors.lastname: Tefsen
authors.forename: Harm
authors.initials: H
authors.lastname: Snippe
authors.forename: Irma
authors.initials: I
authors.lastname: van Die
authors.forename: Soledad
authors.initials: S
authors.lastname: Penadés
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology; D000818:Animals; D000907:Antibodies, Bacterial / Q000737:chemistry* / Q000276:immunology; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D005260:Female; D015483:HIV Antibodies / Q000737:chemistry* / Q000276:immunology; D015497:HIV-1 / Q000737:chemistry / Q000276:immunology; D006801:Humans; D008297:Male; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000276:immunology; D022541:Streptococcal Vaccines / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000737:chemistry / Q000276:immunology
chemical_list: D016915:AIDS Vaccines; D000907:Antibodies, Bacterial; D000939:Epitopes; D015483:HIV Antibodies; D011135:Polysaccharides, Bacterial; D022541:Streptococcal Vaccines