title: Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate.
abstract: To minimize the change of lignocellulosic hydrolysate composition during storage, the effects of storage conditions (temperature, pH and time) on the composition and fermentability of hydrolysate prepared from AFEX™ (Ammonia Fiber Expansion - a trademark of MBI, Lansing, MI) pretreated corn stover were investigated. Precipitates formed during hydrolysate storage increased with increasing storage pH and time. The precipitate amount was the least when hydrolysate was stored at 4 °C and pH 4.8, accounting for only 0.02% of the total hydrolysate weight after 3-month storage. No significant changes of NMR (Nuclear Magnetic Resonance) spectra and concentrations of sugars, minerals and heavy metals were observed after storage under this condition. When pH was adjusted higher before fermentation, precipitates also formed, consisting of mostly struvite (MgNH4PO4·6H2O) and brushite (CaHPO4·2H2O). Escherichia coli and Saccharomyces cerevisiae fermentation studies and yeast cell growth assays showed no significant difference in fermentability between fresh hydrolysate and stored hydrolysate.
authors.affiliation: Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, 3900 Collins Road, Lansing, MI 48910, United States; DOE Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI 48824, United States. Electronic address: jinmingj@egr.msu.edu.
authors.forename: Mingjie
authors.initials: M
authors.lastname: Jin
authors.affiliation: DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, 1552 University Ave., Madison, WI 53706, United States.
authors.forename: William
authors.initials: W
authors.lastname: Bothfeld
authors.affiliation: DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, 1552 University Ave., Madison, WI 53706, United States; Department of Civil and Environmental Engineering, University of Wisconsin-Madison, Madison, WI 53706, United States.
authors.forename: Samantha
authors.initials: S
authors.lastname: Austin
authors.affiliation: DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, 1552 University Ave., Madison, WI 53706, United States.
authors.forename: Trey K
authors.initials: TK
authors.lastname: Sato
authors.affiliation: DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, 1552 University Ave., Madison, WI 53706, United States.
authors.forename: Alex
authors.initials: A
authors.lastname: La Reau
authors.affiliation: DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, 1552 University Ave., Madison, WI 53706, United States.
authors.forename: Haibo
authors.initials: H
authors.lastname: Li
authors.affiliation: DOE BioEnergy Science Center, Washington University in St. Louis, 1 Brookings Drive, St. Louis, MO 63130, United States.
authors.forename: Marcus
authors.initials: M
authors.lastname: Foston
authors.affiliation: Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, 3900 Collins Road, Lansing, MI 48910, United States; DOE Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI 48824, United States.
authors.forename: Christa
authors.initials: C
authors.lastname: Gunawan
authors.affiliation: DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, 1552 University Ave., Madison, WI 53706, United States.
authors.forename: Richard D
authors.initials: RD
authors.lastname: LeDuc
authors.affiliation: Center for Microbial Ecology, Michigan State University, 540 Plant and Soil Science Bldg, East Lansing, MI 48824, United States.
authors.forename: John F
authors.initials: JF
authors.lastname: Quensen
authors.affiliation: DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, 1552 University Ave., Madison, WI 53706, United States.
authors.forename: Mick
authors.initials: M
authors.lastname: Mcgee
authors.affiliation: Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, 3900 Collins Road, Lansing, MI 48910, United States; DOE Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI 48824, United States.
authors.forename: Nirmal
authors.initials: N
authors.lastname: Uppugundla
authors.affiliation: DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, 1552 University Ave., Madison, WI 53706, United States.
authors.forename: Alan
authors.initials: A
authors.lastname: Higbee
authors.affiliation: DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, 1552 University Ave., Madison, WI 53706, United States.
authors.forename: Ruwan
authors.initials: R
authors.lastname: Ranatunga
authors.affiliation: Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, 3900 Collins Road, Lansing, MI 48910, United States; DOE Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI 48824, United States.
authors.forename: Charles W
authors.initials: CW
authors.lastname: Donald
authors.affiliation: DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, 1552 University Ave., Madison, WI 53706, United States.
authors.forename: Gwen
authors.initials: G
authors.lastname: Bone
authors.affiliation: DOE BioEnergy Science Center, Georgia Institute of Technology, 500 10th Street, Atlanta, GA 30332, United States.
authors.forename: Arthur J
authors.initials: AJ
authors.lastname: Ragauskas
authors.affiliation: Center for Microbial Ecology, Michigan State University, 540 Plant and Soil Science Bldg, East Lansing, MI 48824, United States.
authors.forename: James M
authors.initials: JM
authors.lastname: Tiedje
authors.affiliation: DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, 1552 University Ave., Madison, WI 53706, United States; Department of Civil and Environmental Engineering, University of Wisconsin-Madison, Madison, WI 53706, United States.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Noguera
authors.affiliation: Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, 3900 Collins Road, Lansing, MI 48910, United States; DOE Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI 48824, United States.
authors.forename: Bruce E
authors.initials: BE
authors.lastname: Dale
authors.affiliation: DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, 1552 University Ave., Madison, WI 53706, United States. Electronic address: yzhang8@wisc.edu.
authors.forename: Yaoping
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, 3900 Collins Road, Lansing, MI 48910, United States; DOE Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI 48824, United States. Electronic address: balan@egr.msu.edu.
authors.forename: Venkatesh
authors.initials: V
authors.lastname: Balan
mesh_terms: D004795:Enzyme Stability; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013696:Temperature
chemical_list: C036909:lignocellulose; D008031:Lignin
keywords: AFEX; Hydrolysate characterization; Hydrolysate fermentation; Hydrolysate precipitate; Hydrolysate storage