title: Binding investigation on the interaction between Methylene Blue (MB)/TiO2 nanocomposites and bovine serum albumin by resonance light-scattering (RLS) technique and fluorescence spectroscopy.
abstract: The interaction between Methylene Blue (MB)/TiO2 nanocomposites and bovine serum albumin (BSA) was investigated by resonance light scattering (RLS), fluorescence, three-dimension spectra and UV-vis absorbance spectroscopy. Several factors which may influence the RLS intensity were also investigated before characterizing MB/TiO2-BSA complex. It was proved that the mechanism of MB/TiO2 nanocomposites binding to BSA was mainly a result of the formation of MB/TiO2-BSA complex. The binding constant of MB/TiO2-BSA is 0.762 × 10(-5) L mol(-1) at 298K. By calculating the binding constant at different temperature, the thermodynamic parameters ΔH, ΔG, and ΔS can be observed and deduced that the hydrophobic interactions played an important role to stabilize the complex. The distance r (3.73 nm) between donor (BSA) and acceptor (MB/TiO2) was obtained according to fluorescence resonance energy transfer (FRET). The binding site for MB/TiO2 on BSA was mainly located in sub-domain IIA. The UV-vis absorbance, circular dichroism and three dimension fluorescence have also been used to investigate the effect of MB/TiO2 on the conformation of BSA.
authors.affiliation: Non-power Nuclear Technology Collaborative Innovation Center, Hubei University of Science & Technology, Xianning 437100, PR China; State Key Laboratory of Virology & Key Laboratory of Analytical Chemistry for Biology and Medicine (MOE), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China. Electronic address: frank78929@163.com.
authors.forename: Yuesheng
authors.initials: Y
authors.lastname: Li
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shaofa
authors.initials: S
authors.lastname: Sun
authors.forename: Aiqing
authors.initials: A
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D031541:Fluorescence Resonance Energy Transfer; D006863:Hydrogen-Ion Concentration; D008027:Light; D008751:Methylene Blue / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D011485:Protein Binding; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013816:Thermodynamics; D014025:Titanium / Q000737:chemistry*
chemical_list: C009495:titanium dioxide; D012710:Serum Albumin, Bovine; D014025:Titanium; D008751:Methylene Blue
keywords: Bovine serum albumin; Fluorescence; Interaction; MB/TiO(2) nanocomposites; Resonance light scattering