title: Ovatodiolide inhibits the maturation of allergen-induced bone marrow-derived dendritic cells and induction of Th2 cell differentiation.
abstract: Ovatodiolide was a unique macrocyclic diterpenoid isolated from the traditional Chinese medicinal herb Anisomeles indica. The present study attempted to examine the ovatodiolide effects on dendritic cell (DC) maturation and immuno-stimulatory activities. The effects of ovatodiolide on DC surface molecule expression, cytokine production, and capacity to induce T-cell differentiation were examined in ovalbumin (OVA)/thymic stromal lymphopoietin (TSLP)-stimulated DCs. Ovatodiolide attenuated the expression of DC surface molecules CD80, CD86, histocompatibility complex (MHC) class II, and Th2 subset of CD4(+) T cells co-stimulatory molecule-OX40 ligand production. Additionally, ovatodiolide suppressed the CD4(+) T cells proliferation, and production of inflammatory cytokines interleukin (IL)-4, IL-5, and tumor necrosis factor (TNF)-α. This study may be useful to develop ovatodiolide as a therapeutic adjuvant.
authors.affiliation: Institute of Biochemical Sciences and Technology, Chaoyang University of Technology, Taichung, Taiwan.
authors.forename: Yerra Koteswara
authors.initials: YK
authors.lastname: Rao
authors.forename: Yu-Ching
authors.initials: YC
authors.lastname: Chen
authors.forename: Shih-Hua
authors.initials: SH
authors.lastname: Fang
authors.forename: Chih-Ho
authors.initials: CH
authors.lastname: Lai
authors.forename: Madamanchi
authors.initials: M
authors.lastname: Geethangili
authors.forename: Chen-Chen
authors.initials: CC
authors.lastname: Lee
authors.forename: Yew-Min
authors.initials: YM
authors.lastname: Tzeng
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D003713:Dendritic Cells / Q000187:drug effects* / Q000502:physiology; D004224:Diterpenes / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D053278:OX40 Ligand; D010047:Ovalbumin / Q000276:immunology; D018418:Th2 Cells / Q000166:cytology / Q000187:drug effects*; D048069:Tumor Necrosis Factors / Q000276:immunology; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D000485:Allergens; D000954:Antigens, Surface; D016207:Cytokines; D004224:Diterpenes; D008562:Membrane Glycoproteins; D053278:OX40 Ligand; C504586:Tnfsf4 protein, mouse; D048069:Tumor Necrosis Factors; C432447:ovatodiolide; D010047:Ovalbumin; D000094632:Thymic Stromal Lymphopoietin; C000721967:TSLP protein, mouse
keywords: CD80; CD86; Dendritic cell maturation; Inflammatory cytokines; MHC class II; Ovatodiolide