title: Facile method for large scale alignment of one dimensional nanoparticles and control over myoblast orientation and differentiation.
abstract: A facile and robust method to align one-dimensional (1D) nanoparticles (NPs) in large scale has been developed. Using flow assembly, representative rod-like nanoparticles, including tobacco mosaic virus (TMV), gold nanorods, and bacteriophage M13, have been aligned inside glass tubes by controlling flow rate and substrate surface properties. The properties of 1D NPs, such as stiffness and aspect ratio, play a critical role in the alignment. Furthermore, these hierarchically organized structures can be used to support cell growth and control the cell orientation and morphology. When C2C12 myoblasts were cultured on surfaces coated with aligned TMV, we found that nanoscale topographic features were critical to guide the cell orientation and myogenic differentiation. This method can therefore be used in the fabrication of complex assemblies with 1D NPs and have wide applications in tissue engineering, sensing, electronics, and optical fields.
authors.affiliation: Department of Chemistry and Biochemistry, University of South Carolina , Columbia, South Carolina 29208, United States.
authors.forename: Xingjie
authors.initials: X
authors.lastname: Zan
authors.forename: Sheng
authors.initials: S
authors.lastname: Feng
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Balizan
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000818:Animals; D017104:Bacteriophage M13 / Q000737:chemistry; D002454:Cell Differentiation; D002460:Cell Line; D006046:Gold / Q000737:chemistry; D051379:Mice; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D032446:Myoblasts / Q000166:cytology*; D053758:Nanoparticles; D014027:Tobacco Mosaic Virus / Q000737:chemistry
chemical_list: D006046:Gold