title: Mdodeling a nitrite-dependent anaerobic methane oxidation process: parameters identification and model evaluation.
abstract: Nitrite-dependent anaerobic methane oxidation (n-damo) is a recently discovered process that is intermediated by n-damo bacteria that oxidize methane with nitrite to generate nitrogen gas. In this work, a kinetic model based on Monod type kinetics and diffusion-reaction model was developed to describe the bioprocess. Some key kinetic parameters needed in the model were obtained from a series of batch activity tests and a sequencing batch reactor (SBR) operation over 100 days. The growth rate, decay rate, methane affinity constant, nitrite affinity constant and inhibition constant were 0.0277±0.0022 d(-1), 0.00216±0.00010 d(-1), 0.092±0.005 mmol L(-1), 0.91±0.09 mmol L(-1) and 4.1±0.5 mmol L(-1) for n-damo bacteria at 30 °C, respectively. The results showed that the model could simulate actual performance of the SBR in the first 76 days, that methane was not a limiting factor at atmospheric pressure for its high affinity, and that the optimum nitrite concentration was 1.92 mmol L(-1).
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China.
authors.forename: Zhanfei
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Cai
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Geng
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Lou
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zheng
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China. Electronic address: blhu@zju.edu.cn.
authors.forename: Baolan
authors.initials: B
authors.lastname: Hu
mesh_terms: D000693:Anaerobiosis; D018533:Biomass; D007700:Kinetics; D008697:Methane / Q000378:metabolism*; D008962:Models, Theoretical; D010084:Oxidation-Reduction
chemical_list: D008697:Methane
keywords: Anaerobic methane oxidation; Kinetic parameter; Model; N-damo