title: Regulation of microRNA-1288 in colorectal cancer: altered expression and its clinicopathological significance.
abstract: We aim to examine the miR-1288 expression in cancer cell lines and a large cohort of patients with colorectal cancer. Two colon cancer cell lines (SW480 and SW48) and one normal colonic epithelial cell line (FHC) were recruited. The miRNA expressions of miR-1288 were tested on these cell lines by using quantitative real-time polymerase chain reaction (qRT-PCR). An exogenous miR-1288 (mimic) was used to detect cell proliferation and cell cycle changes in SW480 using MTT calorimetric assay and flow cytometry, respectively. In addition, tissues from 122 patients with surgical resection of colorectum (82 adenocarcinomas, 20 adenomas, and 20 non-neoplastic tissues) were tested for miR-1288 expression by qRT-PCR. The colon cancer cell lines showed reduced expression of miR-1288 compared to normal colonic epithelial cell line. Over expression of miR-1288 in SW480 cell line showed increased cell proliferation and increased G2-M phase cells. In tissues, reduced miR-1288 expression was noted in majority of colorectal adenocarcinoma compared to colorectal adenoma and non-neoplastic tissues. Reduced or absent expression of miR-1288 was noted in 76% (n = 62/82) of the cancers. The expression levels of miR-1288 were higher in distal colorectal adenocarcinomas (P = 0.013) and in cancers of lower T staging (P = 0.033). To conclude, alternation of miR-1288 expression is important in the progression of colorectal cancer. The differential regulation of miR-1288 was found to be related to cancer location and pathological staging in colorectal cancers.
authors.affiliation: Cancer Molecular Pathology of Griffith Health Institute, Griffith University, Gold Coast, Queensland, Australia.
authors.forename: Vinod
authors.initials: V
authors.lastname: Gopalan
authors.forename: Suja
authors.initials: S
authors.lastname: Pillai
authors.forename: Faeza
authors.initials: F
authors.lastname: Ebrahimi
authors.forename: Ali
authors.initials: A
authors.lastname: Salajegheh
authors.forename: Tommy C
authors.initials: TC
authors.lastname: Lam
authors.forename: Tran K
authors.initials: TK
authors.lastname: Le
authors.forename: Nicole
authors.initials: N
authors.lastname: Langsford
authors.forename: Yik-Hong
authors.initials: YH
authors.lastname: Ho
authors.forename: Robert A
authors.initials: RA
authors.lastname: Smith
authors.forename: Alfred K-Y
authors.initials: AK
authors.lastname: Lam
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000401:mortality / Q000556:secondary; D000236:Adenoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D015153:Blotting, Western; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D009364:Neoplasm Recurrence, Local / Q000188:drug therapy / Q000473:pathology; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate; D014407:Tumor Cells, Cultured
chemical_list: C576906:MIRN1228 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger
keywords: MicroRNA; cell cycle; cell proliferation; colorectum; miR-1288