title: Histochemical study of the hepatopancreas in adult females of the pink-shrimp Farfantepenaeus brasiliensis Latreille, 1817.
abstract: This study provides histochemical data of the hepatopancreatic cells of adult female pink-shrimp (Farfantepenaeus brasiliensis) at two different developmental stages (those with developed gonads and those with exhausted gonads). The F. brasiliensis females were collected in seawater off the Guarapari coast, Espirito Santo, Brazil. Five cell types were identified in this digestive gland: B (vesicular), E (embryonic), F (fibrillar), M (basal) and R (resorptive). The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids). Acid glycoconjugates were found inside vacuoles in the R cells, while neutral polysaccharides were present in the B cells and near to the microvilli. In females with exhausted gonads polysaccharides were also seen in the intertubular spaces and inside the lumina of the tubules. The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells. The bound calcium was mainly found in the F and M cells. The F cells showed strong positive staining for lipid while the R cell only stained weakly. The E cells did not react to any of the applied staining techniques. The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis. Also, they may be more related to the molt stage, as in the case of calcium salts.
authors.affiliation: Departamento de Biologia, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo, Alto Universitário, s/n(o), Alegre, ES 29500-000, Brazil. Electronic address: erikatnunes@yahoo.com.br.
authors.forename: Erika Takagi
authors.initials: ET
authors.lastname: Nunes
authors.affiliation: Departamento de Biologia, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo, Alto Universitário, s/n(o), Alegre, ES 29500-000, Brazil.
authors.forename: Adriane Araújo
authors.initials: AA
authors.lastname: Braga
authors.affiliation: Departamento de Biologia, Instituto de Biociências, Universidade Estadual Paulista, Av. 24A, 1515, Bela Vista, Rio Claro, SP 13506-765, Brazil.
authors.forename: Maria Izabel
authors.initials: MI
authors.lastname: Camargo-Mathias
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D005260:Female; D043143:Hepatopancreas / Q000166:cytology / Q000378:metabolism; D033561:Penaeidae / Q000166:cytology* / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism
chemical_list: D060829:Arthropod Proteins; D011134:Polysaccharides; D002118:Calcium
keywords: Digestive gland; Farfantepenaeus brasiliensis; Hepatopancreas; Histochemistry