title: Characterisation and stability evaluation of bixin nanocapsules.
abstract: The aim of this study was to produce bixin nanocapsules by the interfacial deposition of preformed poly-ε-caprolactone (PCL). PCL (250 mg), capric/caprylic triglyceride (400 μL), sorbitan monostearate (95 mg) and bixin were dissolved in a mixture of acetone (60 mL) and ethanol (7.5 mL) under stirring (40 °C). This organic solution was added to the aqueous solution (130 mL) containing Tween 80 (195 mg). The size distributions in the formulations with bixin concentration from 11 to 100 μg/mL were evaluated periodically during 3 weeks of storage at ambient temperature. The optimal formulation (bixin concentration of 16.92±0.16 μg/mL) was characterised in terms of particle size distribution, zeta potential, bixin content and encapsulation efficiency, and showed a volume-weighted mean diameter (D4,3) of 195±27 nm, around 100% of encapsulation efficiency and the nanocapsules were considered physically stable during 119 days of storage at ambient temperature.
authors.affiliation: Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, n. 9500, CEP 91501-970, Porto Alegre, RS, Brazil.
authors.forename: Kleidson Brito de Sousa
authors.initials: KB
authors.lastname: Lobato
authors.forename: Karina
authors.initials: K
authors.lastname: Paese
authors.forename: Joana Casanova
authors.initials: JC
authors.lastname: Forgearini
authors.forename: Silvia Stanisçuaski
authors.initials: SS
authors.lastname: Guterres
authors.forename: André
authors.initials: A
authors.lastname: Jablonski
authors.forename: Alessandro de Oliveira
authors.initials: Ade O
authors.lastname: Rios
mesh_terms: D002338:Carotenoids / Q000737:chemistry*; D004339:Drug Compounding / Q000379:methods*; D004355:Drug Stability; D004356:Drug Storage; D053769:Nanocapsules / Q000737:chemistry*; D010316:Particle Size
chemical_list: D053769:Nanocapsules; D002338:Carotenoids; C004588:bixin
keywords: Bioactive compounds; Carotenoid; Lipid-core nanocapsules; Nanoencapsulation