title: Restrictionless cloning.
abstract: Many methods have been developed for the cloning of PCR products. These methods include blunt-end cloning, TA cloning, and using restriction sites incorporated into the PCR primers. The restrictionless cloning technique allows efficient directional cloning of PCR products into any cloning site within a vector regardless of whether the sites are contained within the insert to be cloned.
authors.affiliation: J. Craig Venter Institute, Synthetic Biology Group, Rockville, MD, USA. Electronic address: malgire@jcvi.org.
authors.forename: Mikkel A
authors.initials: MA
authors.lastname: Algire
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers
keywords: Denaturation and annealing; Ligation and transformation; Polymerase chain reaction (PCR); Primer design; Restrictionless cloning; Vector preparation