title: Effects of 90-day feeding of transgenic Bt rice TT51 on the reproductive system in male rats.
abstract: Rice is a staple food crop; however, the threat of pests leads to a serious decline in its output and quality. The CryAb/CryAc gene, encodes a synthetic fusion Bacillus thuringiensis (Bt) crystal protein, was introduced into rice MingHui63 to produce insect-resistant rice TT51. This study was undertaken to investigate potential unintended effects of TT51 on the reproductive system in male rats. Male rats were treated with diets containing 60% of either TT51 or MingHui63 by weight, nutritionally balanced to an AIN93G diet, for 90days. An additional negative control group of rats were fed with a rice-based AIN93G diet. Body weights, food intake, hematology, serum chemistry, serum hormone levels, sperm parameters and relative organ/body weights were measured, and gross as well as microscopic pathology were examined. No diet-related significant differences in the values of response variables were observed between rats that were fed with diet containing transgenic TT51, MingHui63 and the control in this 90-day feeding study. In addition, necropsy and histopathology examination indicated no treatment-related changes. The results from the present study indicated that TT51 does not appear to exert any effect on the reproductive system in male rats compared with MingHui63 or the control.
authors.affiliation: National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, No. 7 Pan Jia Yuan Nan Li, Beijing 100021, China; National Center for Food Safety Risk Assessment, Beijing, China.
authors.forename: Er Hui
authors.initials: EH
authors.lastname: Wang
authors.forename: Zhou
authors.initials: Z
authors.lastname: Yu
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.forename: Hai Bin
authors.initials: HB
authors.lastname: Xu
mesh_terms: D000821:Animal Feed / Q000009:adverse effects / Q000633:toxicity*; D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics; D001769:Blood / Q000187:drug effects; D001774:Blood Chemical Analysis; D001835:Body Weight / Q000187:drug effects; D004731:Endotoxins / Q000235:genetics; D012739:Gonadal Steroid Hormones / Q000097:blood; D006460:Hemolysin Proteins / Q000235:genetics; D008297:Male; D009929:Organ Size / Q000187:drug effects; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified; D051381:Rats; D017208:Rats, Wistar; D012098:Reproduction / Q000187:drug effects; D013081:Sperm Motility / Q000187:drug effects; D018675:Toxicity Tests / Q000379:methods
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D012739:Gonadal Steroid Hormones; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis
keywords: Bacillus thuringiensis; Reproductive toxicity; Sperm parameter; Transgenic rice