title: Peptidolytic Microbial Community of Methanogenic Reactors from two Modified Uasbs of Brewery Industries.
abstract: We studied the peptide-degrading anaerobic communities of methanogenic reactors from two mesophilic full-scale modified upflow anaerobic sludge blanket (UASB) reactors treating brewery wastewater in Colombia. Most probable number (MPN) counts varied between 7.1 x 10(8) and 6.6 × 10(9) bacteria/g volatile suspended solids VSS (Methanogenic Reactor 1) and 7.2 × 10(6) and 6.4 × 10(7) bacteria/g (VSS) (Methanogenic Reactor 2). Metabolites detected in the highest positive MPN dilutions in both reactors were mostly acetate, propionate, isovalerate and, in some cases, negligible concentrations of butyrate. Using the highest positive dilutions of MPN counts, 50 dominant strains were isolated from both reactors, and 12 strains were selected for sequencing their 16S rRNA gene based on their phenotypic characteristics. The small-subunit rRNA gene sequences indicated that these strains were affiliated to the families Propionibacteriaceae, Clostridiaceae and Syntrophomonadaceae in the low G + C gram-positive group and Desulfovibrio spp. in the class δ-Proteobacteria. The main metabolites detected in the highest positive dilutions of MPN and the presence of Syntrophomonadaceae indicate the effect of the syntrophic associations on the bioconversion of these substrates in methanogenic reactors. Additionally, the potential utilization of external electron acceptors for the complete degradation of amino acids by Clostridium strains confirms the relevance of these acceptors in the transformation of peptides and amino acids in these systems.
authors.affiliation: Unidad de Saneamiento y Biotecnología Ambiental (USBA), Departamento de Biología , Pontificia Universidad Javeriana, P.O.B. 56710, Bogotá , Colombia.
authors.forename: C
authors.initials: C
authors.lastname: Díaz
authors.forename: S
authors.initials: S
authors.lastname: Baena
authors.forename: B K C
authors.initials: BK
authors.lastname: Patel
authors.forename: M L
authors.initials: ML
authors.lastname: Fardeau
keywords: 16S rRNA gene; MPN; UASB; anaerobic peptide-degrading bacteria
references.citation: Water Res. 2002 Apr;36(7):1767-75
references.pmid: 12044076
references.citation: Appl Environ Microbiol. 2005 Apr;71(4):2145-53
references.pmid: 15812049
references.citation: Water Res. 2007 Apr;41(7):1554-68
references.pmid: 17291558
references.citation: Syst Appl Microbiol. 2005 Mar;28(2):175-85
references.pmid: 15830810
references.citation: J Biosci Bioeng. 2005 Feb;99(2):150-64
references.pmid: 16233772
references.citation: Int J Syst Bacteriol. 1999 Jul;49 Pt 3:975-82
references.pmid: 10425753
references.citation: Microbiol Mol Biol Rev. 1997 Jun;61(2):262-80
references.pmid: 9184013
references.citation: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references.pmid: 9254694
references.citation: Evolution. 1985 Jul;39(4):783-791
references.pmid: 28561359
references.citation: Int J Syst Evol Microbiol. 2007 Aug;57(Pt 8):1914-1918
references.pmid: 17684281
references.citation: Anaerobe. 2007 Jun-Aug;13(3-4):99-106
references.pmid: 17631395
references.citation: Environ Microbiol. 2005 Feb;7(2):213-24
references.pmid: 15658988
references.citation: Int J Syst Evol Microbiol. 2002 Mar;52(Pt 2):401-407
references.pmid: 11931148
references.citation: FEMS Microbiol Lett. 1993 Oct 1;113(1):81-6
references.pmid: 8243987
references.citation: Nucleic Acids Res. 1997 Jan 1;25(1):111-6
references.pmid: 9016516
references.citation: Syst Appl Microbiol. 1998 Dec;21(4):498-504
references.pmid: 9924817
references.citation: Biodegradation. 2001;12(4):247-57
references.pmid: 11826907
references.citation: Int J Syst Bacteriol. 1997 Jul;47(3):818-24
references.pmid: 9226912
references.citation: Appl Microbiol. 1974 May;27(5):985-7
references.pmid: 4598231
references.citation: Int J Syst Evol Microbiol. 2002 Sep;52(Pt 5):1461-1468
references.pmid: 12361247
references.citation: FEMS Microbiol Lett. 1997 Oct 15;155(2):169-77
references.pmid: 9351198
references.citation: Appl Environ Microbiol. 2006 Jul;72(7):4942-9
references.pmid: 16820491
references.citation: Microbiology (Reading). 1998 Sep;144 ( Pt 9):2655-2665
references.pmid: 9782515
references.citation: Int J Syst Evol Microbiol. 2000 Jan;50 Pt 1:259-264
references.pmid: 10826812
references.citation: Mol Biol Evol. 1987 Jul;4(4):406-25
references.pmid: 3447015
references.citation: Int J Syst Evol Microbiol. 2002 May;52(Pt 3):795-800
references.pmid: 12054240
references.citation: Antonie Van Leeuwenhoek. 1994;66(1-3):271-94
references.pmid: 7747937
references.citation: J Environ Sci (China). 2008;20(5):626-31
references.pmid: 18575118
references.citation: Int J Syst Evol Microbiol. 2008 Sep;58(Pt 9):2003-12
references.pmid: 18768595
references.citation: Anaerobe. 2004 Jun;10(3):185-90
references.pmid: 16701517
references.citation: Microbiology (Reading). 2001 Feb;147(Pt 2):373-382
references.pmid: 11158354
references.citation: FEMS Microbiol Lett. 2003 Feb 28;219(2):297-304
references.pmid: 12620635
references.citation: Appl Environ Microbiol. 1997 Jul;63(7):2802-13
references.pmid: 9212428
references.citation: Microb Ecol. 2008 Jul;56(1):121-32
references.pmid: 18034358
references.citation: Environ Microbiol. 2000 Oct;2(5):506-15
references.pmid: 11233159
references.citation: Bioresour Technol. 2006 Jul;97(10):1201-8
references.pmid: 16055329