title: Ethanol fermentation of sugarcane molasses by Zymomonas mobilis MTCC 92 immobilized in Luffa cylindrica L. sponge discs and Ca-alginate matrices.
abstract: Bio-ethanol production from cane molasses (diluted to 15 % sugar w/v) was studied using the bacterium, Zymomonas mobilis MTCC 92 entrapped in luffa (Luffa cylindrica L.) sponge discs and Ca-alginate gel beads as the immobilizing matrices. At the end of 96 h fermentation, the final ethanol concentrations were 58.7 ± 0.09 and 59.1 ± 0.08 g/l molasses with luffa and Ca-alginate entrapped Z. mobilis cells, respectively exhibiting 83.25 ± 0.03 and 84.6 ± 0.02 % sugar conversion. There was no statistical significant difference (Fischer's LSD) in sugar utilization (t = 0.254, p<0.801) and ethanol production (t =-0.663, p<0.513) between the two immobilization matrices used. Further, the immobilized cells in both the matrices were physiologically active for three more cycles of operation with less than 15 % decrease in ethanol yield in the 4(th) cycle, which was due to some leakage of cells. In conclusion, luffa sponge was found to be equally good as Ca-alginate as a carrier material for bacterial (Z. mobilis) cell immobilization for ethanol production. Further, it has added advantages such as it is cheap, non-corrosive and has no environmental hazard.
authors.affiliation: Department of Botany, Utkal University , Vanivihar, Bhubaneswar -751004, Orissa , India.
authors.forename: Shuvashish
authors.initials: S
authors.lastname: Behera
authors.forename: Rama C
authors.initials: RC
authors.lastname: Mohanty
authors.forename: Ramesh C
authors.initials: RC
authors.lastname: Ray
keywords: Bio-ethanol; Cell immobilization; Fermentation; Molasses; Zymomonas mobilis MTCC 92
references.citation: Z Naturforsch C J Biosci. 1998 Nov-Dec;53(11-12):968-72
references.pmid: 9933964
references.citation: AAPS PharmSciTech. 2005 Oct 19;6(3):E391-7
references.pmid: 16353996
references.citation: Braz J Microbiol. 2011 Apr;42(2):676-84
references.pmid: 24031679
references.citation: Bioresour Technol. 2001 Jan;76(1):1-8
references.pmid: 11315804
references.citation: Braz J Microbiol. 2009 Oct;40(4):715-24
references.pmid: 24031418
references.citation: Chemosphere. 2005 Oct;61(4):510-8
references.pmid: 16202804
references.citation: J Biosci Bioeng. 2001;92(6):560-4
references.pmid: 16233146
references.citation: Pol J Microbiol. 2008;57(1):49-57
references.pmid: 18610656
references.citation: Appl Biochem Biotechnol. 2009 Feb;152(2):177-88
references.pmid: 18521760
references.citation: J Biosci Bioeng. 2007 Sep;104(3):214-7
references.pmid: 17964486
references.citation: Biotechnol Bioeng. 1994 Feb 5;43(3):189-94
references.pmid: 18615650
references.citation: J Biotechnol. 2000 Jun 23;80(2):189-93
references.pmid: 10908799
references.citation: Braz J Microbiol. 2010 Oct;41(4):931-45
references.pmid: 24031573
references.citation: Bioresour Technol. 2007 Nov;98(15):2824-8
references.pmid: 17420121