title: Stable knockdown of hCGβ mRNA expression in bladder cancer cells results in significant growth inhibition.
abstract: BACKGROUND
  Expression of human chorionic gonadotropin beta subunit (hCGβ) by epithelial carcinomas is associated with a poor prognosis and has a proposed autocrine growth effect on cancer cells by inhibition of apoptosis.
  
  
  METHODS
  We transduced the hCGβ-expressing bladder cancer cell line SCaBER with short hairpin (sh) RNA lentiviral gene-specific (CGB) constructs and determined its impact on the synthesis of hCGβ and the resultant effect on cancer cell growth.
  
  
  RESULTS
  Stable CGB gene-silenced clones exhibited a 60%-80% reduction in the level of hCGβ expressed and a reduced growth rate of more than 40% compared to wild-type SCaBER cells.
  
  
  CONCLUSIONS
  shRNA Lentiviral particles achieve stable knockdown of hCGβ translation in the bladder cancer cell line SCaBER. This transforms the phenotype by reducing hCGβ expression and cell growth rate. This is consistent with the proposed autocrine/paracrine function of ectopic hCGβ expression during oncogenesis.
authors.affiliation: ELK Foundation for Health Research, An Scoil Monzaird, Crieff, Scotland, PH7 4JT, U.K. ray@iles.net.
authors.forename: Beata
authors.initials: B
authors.lastname: Burczynska
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Booth
authors.forename: Ray K
authors.initials: RK
authors.lastname: Iles
authors.forename: Ankita
authors.initials: A
authors.lastname: Shah
authors.forename: Ashref
authors.initials: A
authors.lastname: Shiled
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Butler
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000235:genetics*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D018997:Chorionic Gonadotropin, beta Subunit, Human; D012333:RNA, Messenger; D034741:RNA, Small Interfering
keywords: Lentiviral siRNA; bladder carcinoma; hCGβ; silencing; stable gene knockdown SCaBER cells