title: Identification and field evaluation of non-host volatiles disturbing host location by the tea geometrid, Ectropis obliqua.
abstract: Volatile organic compounds derived from non-host plants, Ocimum basilicum, Rosmarinus officinalis, Corymbia citriodora, and Ruta graveolens, can be used to mask host plant odors, and are repellent to the tea geometrid, Ectropis obliqua. Volatile compounds were collected by headspace absorption, and the components were identified and quantified by using gas chromatography/mass spectrometry. The responses of antennae of female E. obliqua to the compounds were evaluated with gas chromatography/electroantennography detection. Qualitative and quantitative differences were found among the four odor profiles. Consistent electroantennographic activity was obtained for eight of the volatiles from the four plants: β-myrcene, α-terpinene, γ-terpinene, linalool, cis-verbenol, camphor, α-terpineol, and verbenone. In a Y-tube bioassay, six chemicals, β-myrcene, γ-terpinene, (R)-(-)-linalool, (S)-(-)-cis-verbenol, (R)-(+)-camphor, and (S)-(-)-verbenone, were the main compounds responsible for repelling E. obliqua. An eight-component mixture including all of the bioactive compounds (in a ratio of 13:2:13:8:1:24:6:17) from R. officinalis was significantly more effective at repelling the moths than any single compound or a mixture of equal amounts of the eight compounds. Field results demonstrated that intercropping tea plants with R. officinalis effectively suppressed E. obliqua infestations in a tea plantation. Our findings suggests that odor blends of R. officinalis play a role in disturbing host orientation behavior, and in repelling E. obliqua adults, and that R. officinalis should be considered when developing "push-pull" strategies aimed at optimizing the control of E. obliqua with semiochemicals.
authors.affiliation: Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou, 310008, People's Republic of China.
authors.forename: Zheng-qun
authors.initials: ZQ
authors.lastname: Zhang
authors.forename: Xiao-ling
authors.initials: XL
authors.lastname: Sun
authors.forename: Zhao-jun
authors.initials: ZJ
authors.lastname: Xin
authors.forename: Zong-xiu
authors.initials: ZX
authors.lastname: Luo
authors.forename: Yu
authors.initials: Y
authors.lastname: Gao
authors.forename: Lei
authors.initials: L
authors.lastname: Bian
authors.forename: Zong-mao
authors.initials: ZM
authors.lastname: Chen
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D028241:Camellia sinensis; D005260:Female; D007302:Insect Repellents / Q000032:analysis* / Q000494:pharmacology*; D007915:Lepidoptera / Q000187:drug effects*; D008297:Male; D009812:Odorants / Q000032:analysis; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000494:pharmacology*
chemical_list: D007302:Insect Repellents; D055549:Volatile Organic Compounds
references.citation: Trends Plant Sci. 2004 May;9(5):253-61
references.pmid: 15130551
references.citation: Plant Cell. 2003 Dec;15(12):2866-84
references.pmid: 14630967
references.citation: J Chem Ecol. 2013 Apr;39(4):481-93
references.pmid: 23460417
references.citation: Annu Rev Entomol. 2011;56:161-80
references.pmid: 21133760
references.citation: J Chem Ecol. 2005 Jan;31(1):111-22
references.pmid: 15839484
references.citation: Bioresour Technol. 2005 Nov;96(16):1749-57
references.pmid: 16051081
references.citation: Pest Manag Sci. 2011 Dec;67(12):1583-8
references.pmid: 21674753
references.citation: Trends Plant Sci. 2005 Jun;10(6):269-74
references.pmid: 15949760
references.citation: PLoS One. 2011 Feb 09;6(2):e17033
references.pmid: 21347337
references.citation: Pest Manag Sci. 2010 Jun;66(6):664-8
references.pmid: 20205230
references.citation: Annu Rev Entomol. 2006;51:45-66
references.pmid: 16332203
references.citation: Phytochemistry. 2011 Sep;72(13):1605-11
references.pmid: 21596403
references.citation: Biochim Biophys Acta. 2005 May 15;1734(2):91-111
references.pmid: 15904867
references.citation: Phytochemistry. 2002 Nov;61(5):573-8
references.pmid: 12409025
references.citation: Annu Rev Entomol. 2007;52:375-400
references.pmid: 16968206
references.citation: J Chem Ecol. 1990 Nov;16(11):3197-212
references.pmid: 24263303
references.citation: Food Chem. 2008 Sep 15;110(2):501-3
references.pmid: 26049245
references.citation: J Chem Ecol. 2012 Aug;38(8):1003-16
references.pmid: 22730107
references.citation: Parasitol Res. 2010 Nov;107(6):1455-61
references.pmid: 20697909
references.citation: Parasitol Res. 2013 Mar;112(3):991-9
references.pmid: 23224707
references.citation: PLoS One. 2012;7(11):e49256
references.pmid: 23166622
references.citation: Fitoterapia. 2006 Dec;77(7-8):491-4
references.pmid: 16890387
references.citation: Annu Rev Entomol. 2002;47:701-31
references.pmid: 11729089
references.citation: PLoS One. 2012;7(8):e42871
references.pmid: 22900060
references.citation: Pest Manag Sci. 2008 Jan;64(1):8-11
references.pmid: 18022796
references.citation: Plant Physiol. 1999 Oct;121(2):325-32
references.pmid: 10517823
references.citation: J Chem Ecol. 2002 Nov;28(11):2203-19
references.pmid: 12523563
references.citation: BMC Complement Altern Med. 2012 Jun 28;12:81
references.pmid: 22742534
references.citation: Bioresour Technol. 2010 Jan;101(1):372-8
references.pmid: 19729299
references.citation: Phytochemistry. 2009 Apr;70(6):751-8
references.pmid: 19394981
references.citation: Cell Mol Life Sci. 2006 Jul;63(14):1658-76
references.pmid: 16786224
references.citation: PLoS Biol. 2012;10(11):e1001435
references.pmid: 23209379
references.citation: Parasitol Res. 2012 Dec;111(6):2253-63
references.pmid: 22903418
references.citation: J Chem Ecol. 2010 Apr;36(4):388-95
references.pmid: 20349338