title: Iron status and lipopolysaccharide regulate Ndfip1 by activation of nuclear factor-kappa B.
abstract: Nedd4 family interacting protein 1 (Ndfip1) is an adaptor protein for the Nedd4 family of ubiquitin ligases that target proteins for degradation. Recent studies confirmed the role of Ndfip1 as a regulator of iron metabolism and pointed out that Ndfip1 was involved in iron homeostasis by regulating the degradation of iron importer divalent metal transporter 1 (DMT1). However, little is known about how Ndfip1 is regulated. The aim of this article was to investigate the regulation of Ndfip1 levels and the possible mechanisms. In this study, we investigated the effect of various stimuli, including iron status and lipopolysaccharide (LPS) on Ndfip1 expression in MES23.5 dopaminergic cell lines. Results showed that Ndfip1 expression in these cells was enhanced by ferrous iron overload, but not ferric iron overload, and decreased after iron deprivation by deferoxamine. In addition, LPS could significantly increase the expression of Ndfip1. Furthermore, we demonstrated that the regulation of Ndfip1 expression by these various stimuli was achieving by activation of nuclear factor-kappa B. We speculate that iron status and LPS may contribute to the changes of Ndfip1 expression by activation of nuclear factor-kappa B.
authors.affiliation: Department of Physiology, Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological Disorders and State Key Disciplines: Physiology, Medical College of Qingdao University, No. 308 Ningxia Road, Qingdao, 266071, China.
authors.forename: Huamin
authors.initials: H
authors.lastname: Xu
authors.forename: Qing
authors.initials: Q
authors.lastname: Chang
authors.forename: Wenting
authors.initials: W
authors.lastname: Jia
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.forename: Peng
authors.initials: P
authors.lastname: Sun
authors.forename: Junxia
authors.initials: J
authors.lastname: Xie
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D003676:Deferoxamine / Q000494:pharmacology; D005290:Ferric Compounds; D005296:Ferrous Compounds; D005786:Gene Expression Regulation; D036341:Intercellular Signaling Peptides and Proteins; D007501:Iron / Q000378:metabolism* / Q000494:pharmacology; D007502:Iron Chelating Agents / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000644:Quaternary Ammonium Compounds; D000099236:Solute Carrier Family 11, Member 2
chemical_list: D002352:Carrier Proteins; D027682:Cation Transport Proteins; D003676:Deferoxamine; D005290:Ferric Compounds; D005296:Ferrous Compounds; D036341:Intercellular Signaling Peptides and Proteins; D007501:Iron; D007502:Iron Chelating Agents; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D016328:NF-kappa B; D000644:Quaternary Ammonium Compounds; D000099236:Solute Carrier Family 11, Member 2; C499188:Ndfip1 protein, mouse; C020748:ferrous sulfate; C013531:ferric ammonium citrate