title: Live/dead state is not the factor influencing adhesion ability of Bifidobacterium animalis KLDS2.0603.
abstract: Two essential requirements for probiotic bifidobacteria are that they be "live" and have "colonization" ability, following FAO/WHO guideline recommendations. The amount of research on the adhesion ability of bifidobacteria compares poorly with that of other probiotic bacteria, such as lactobacilli. The aim of the present study was to determine how gastrointestinal conditions affect the adhesion ability of bifidobacteria, and to investigate the relationship between the adhesion ability and the live/dead state of bifidobacteria. The adhesion ability of Bifidobacterium animalis KLDS2.0603 that had been subjected to the digestive enzymes, pepsin, trypsin, and proteinase K, was decreased significantly, but these treatments did not significantly change the strain's survival rates, which were 98.78%, 97.60%, and 97.63% respectively. B. animalis KLDS2.0603 subjected to LiCl retained its adhesion ability but had a lower survival rate (59.28%) than the control group (P<0.01). B. animalis KLDS 2.0603 subjected to sodium metaperiodate exhibited higher adhesion ability than the control group (P<0.01), but the bacterial cells were killed totally. The results of transmission electron microscopy and laser scanning confocal microscopy showed that live/dead state of bifidobacteria was not one of the main factors that affected the adhesion ability of bifidobacteira, and that the substances affecting the adhesion ability of bifidobacteria were on the outer surface layer of the bifidobacterial cells. Our results also indicated that the substances related to the adhesion ability of bifidobacteria are proteinaceous. The above results will help us to understand the adhesion and colonization processes of bifidobacteria in the human gastrointestinal tract.
authors.affiliation: Key Laboratory of Dairy Science (Ministry of Education), Northeast Agricultural University, Harbin, 150030, P. R. China.
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.forename: Xiang-Chen
authors.initials: XC
authors.lastname: Meng
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects*; D001644:Bifidobacterium / Q000187:drug effects* / Q000502:physiology*; D018021:Lithium Chloride / Q000378:metabolism*; D050296:Microbial Viability / Q000187:drug effects*; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D010447:Peptide Hydrolases / Q000378:metabolism*; D010504:Periodic Acid / Q000378:metabolism*
chemical_list: D010504:Periodic Acid; C009288:metaperiodate; D010447:Peptide Hydrolases; D018021:Lithium Chloride
references.citation: J Biomed Biotechnol. 2008;2008:357964
references.pmid: 18401442
references.citation: Anaerobe. 2007 Oct-Dec;13(5-6):215-9
references.pmid: 17646115
references.citation: Anaerobe. 2012 Oct;18(5):498-503
references.pmid: 22967793
references.citation: Antonie Van Leeuwenhoek. 2005 Oct-Nov;88(3-4):207-19
references.pmid: 16284927
references.citation: Appl Environ Microbiol. 2008 Aug;74(15):4695-702
references.pmid: 18539800
references.citation: Am J Clin Nutr. 2001 Feb;73(2 Suppl):399S-405S
references.pmid: 11157348
references.citation: J Appl Microbiol. 2010 Sep;109(3):927-35
references.pmid: 20408914
references.citation: Int J Food Microbiol. 2004 Nov 15;96(3):307-13
references.pmid: 15454321
references.citation: Appl Environ Microbiol. 1998 Jan;64(1):21-6
references.pmid: 9435057
references.citation: Curr Microbiol. 2009 Aug;59(2):167-72
references.pmid: 19452211
references.citation: Anaerobe. 2011 Apr;17(2):69-72
references.pmid: 21514395
references.citation: Appl Environ Microbiol. 1994 Dec;60(12):4487-94
references.pmid: 7811085
references.citation: J Appl Microbiol. 2009 Oct;107(4):1230-40
references.pmid: 19486400
references.citation: J Appl Microbiol. 2003;94(6):981-7
references.pmid: 12752805
references.citation: Res Microbiol. 2005 Sep;156(8):887-95
references.pmid: 16024231
references.citation: Appl Environ Microbiol. 1993 Dec;59(12):4121-8
references.pmid: 8285709
references.citation: J Microbiol Methods. 2004 Nov;59(2):211-21
references.pmid: 15369857
references.citation: J Appl Microbiol. 2005;98(2):285-92
references.pmid: 15659182
references.citation: Lett Appl Microbiol. 2000 Dec;31(6):438-42
references.pmid: 11123552
references.citation: J Dairy Res. 2004 Aug;71(3):340-5
references.pmid: 15354581
references.citation: J Dairy Sci. 2007 Jun;90(6):2710-6
references.pmid: 17517710