title: Engineered mitochondrial ferritin as a magnetic resonance imaging reporter in mouse olfactory epithelium.
abstract: We report the design of a MRI reporter gene with applications to non-invasive molecular imaging. We modified mitochondrial ferritin to localize to the cell cytoplasm. We confirmed the efficient cellular processing of this engineered protein and demonstrated high iron loading in mammalian cells. The reporter's intracellular localization appears as distinct clusters that deliver robust MRI contrast. We used this new reporter to image in vivo and ex vivo the gene expression in native olfactory sensory neurons in the mouse epithelium. This robust MRI reporter can facilitate the study of the molecular mechanisms of olfaction and to monitor intranasal gene therapy delivery, as well as a wide range of cell tracking and gene expression studies in living subjects.
authors.affiliation: Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania, United States of America ; Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, Pennsylvania, United States of America.
authors.forename: Bistra
authors.initials: B
authors.lastname: Iordanova
authors.forename: T Kevin
authors.initials: TK
authors.lastname: Hitchens
authors.forename: Clinton S
authors.initials: CS
authors.lastname: Robison
authors.forename: Eric T
authors.initials: ET
authors.lastname: Ahrens
mesh_terms: D000818:Animals; D003287:Contrast Media; D005260:Female; D005293:Ferritins; D017930:Genes, Reporter; D057809:HEK293 Cells; D006801:Humans; D007501:Iron / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D024101:Mitochondrial Proteins; D009831:Olfactory Mucosa / Q000378:metabolism*; D015202:Protein Engineering; D011499:Protein Processing, Post-Translational; D021381:Protein Transport; D011984:Sensory Receptor Cells / Q000166:cytology / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D014161:Transduction, Genetic; D019076:Transgenes / Q000235:genetics
chemical_list: D003287:Contrast Media; D024101:Mitochondrial Proteins; C556634:mitochondrial ferritin, human; D005293:Ferritins; D007501:Iron
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