title: Stable expression and potential use of west nile virus envelope glycoproteins preM/E as antigen in diagnostic tests.
abstract: West Nile virus (WNV) envelope glycoproteins preM/E were stably expressed in baby hamster kidney cells and tested as antigen in a fluorescent antibody assay for WNV antibodies. Sera from horses, mice and chicken immunized with an inactivated WNV vaccine and, less consistently, sera from horses acutely infected with WNV, reacted specifically with viral antigens present in preM/E-expressing cells.
authors.affiliation: Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria , Santa Maria, RS , Brasil.
authors.forename: Juliana Felipetto
authors.initials: JF
authors.lastname: Cargnelutti
authors.forename: Mário Celso Sperotto
authors.initials: MC
authors.lastname: Brum
authors.forename: Rudi
authors.initials: R
authors.lastname: Weiblen
authors.forename: Eduardo Furtado
authors.initials: EF
authors.lastname: Flores
keywords: M/E glycoproteins; WNV; West Nile virus; diagnostic; stable expression
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