title: The integral and extrinsic bioactive proteins in the aqueous extracted soybean oil bodies.
abstract: Soybean oil bodies (OBs), naturally pre-emulsified soybean oil, have been examined by many researchers owing to their great potential utilizations in food, cosmetics, pharmaceutical, and other applications requiring stable oil-in-water emulsions. This study was the first time to confirm that lectin, Gly m Bd 28K (Bd 28K, one soybean allergenic protein), Kunitz trypsin inhibitor (KTI), and Bowman-Birk inhibitor (BBI) were not contained in the extracted soybean OBs even by neutral pH aqueous extraction. It was clarified that the well-known Gly m Bd 30K (Bd 30K), another soybean allergenic protein, was strongly bound to soybean OBs through a disulfide bond with 24 kDa oleosin. One steroleosin isoform (41 kDa) and two caleosin isoforms (27 kDa, 29 kDa), the integral bioactive proteins, were confirmed for the first time in soybean OBs, and a considerable amount of calcium, necessary for the biological activities of caleosin, was strongly bound to OBs. Unexpectedly, it was found that 24 kDa and 18 kDa oleosins could be hydrolyzed by an unknown soybean endoprotease in the extracted soybean OBs, which might give some hints for improving the enzyme-assisted aqueous extraction processing of soybean free oil.
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University , 1800 Lihu Avenue, Wuxi, Jiangsu Province 214122, People's Republic of China.
authors.forename: Luping
authors.initials: L
authors.lastname: Zhao
authors.forename: Yeming
authors.initials: Y
authors.lastname: Chen
authors.forename: Yanyun
authors.initials: Y
authors.lastname: Cao
authors.forename: Xiangzhen
authors.initials: X
authors.lastname: Kong
authors.forename: Yufei
authors.initials: Y
authors.lastname: Hua
mesh_terms: D000485:Allergens / Q000032:analysis* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004655:Emulsions / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D020033:Protein Isoforms / Q000737:chemistry; D013024:Soybean Oil / Q000737:chemistry*; D030262:Soybean Proteins / Q000737:chemistry* / Q000276:immunology; D013025:Glycine max / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014358:Trypsin Inhibitor, Bowman-Birk Soybean / Q000737:chemistry / Q000276:immunology; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000737:chemistry / Q000276:immunology
chemical_list: D000485:Allergens; D004655:Emulsions; D020033:Protein Isoforms; D030262:Soybean Proteins; D014358:Trypsin Inhibitor, Bowman-Birk Soybean; D013024:Soybean Oil; D014360:Trypsin Inhibitor, Kunitz Soybean