title: Evaluation of pneumococcal serotyping by multiplex PCR and quellung reactions.
abstract: Screening of 1,750 pneumococcal isolates for common serotypes by PCR was followed by Quellung reaction analysis of PCR-negative isolates with a comparison to the conventional (Quellung reaction only) approach. PCR agreed with Quellung reaction results for 99% of isolates. The sequential PCR/Quellung reaction algorithm is accurate and more cost-effective than the conventional approach.
authors.affiliation: Cleveland Clinic, Cleveland, Ohio, USA.
authors.forename: Sandra S
authors.initials: SS
authors.lastname: Richter
authors.forename: Kristopher P
authors.initials: KP
authors.lastname: Heilmann
authors.forename: Cassie L
authors.initials: CL
authors.lastname: Dohrn
authors.forename: Fathollah
authors.initials: F
authors.lastname: Riahi
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Diekema
authors.forename: Gary V
authors.initials: GV
authors.lastname: Doern
mesh_terms: D000465:Algorithms; D006801:Humans; D058889:Molecular Typing / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D011008:Pneumococcal Infections / Q000382:microbiology*; D012703:Serotyping / Q000379:methods; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
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