title: Characterization of bone marrow mast cells in acute myeloid leukemia with t(8;21) (q22;q22); RUNX1-RUNX1T1.
abstract: Mast cells are often increased in AML with t(8;21). We analyzed characteristics of mast cells to elucidate their relationship with leukemic blasts. In 31 cases in which the results of KIT mutation analysis were available, five cases showed mutations. None of the cases positive for KIT mutation showed increased mast cells. In five cases with increased mast cells in which targeted-FISH analysis was performed for RUNX1-RUNX1T1, fusion signals demonstrated the translocation in mast cells in all cases. These findings confirm the shared origin between mast cells and leukemic blasts in AML with t(8;21).
authors.affiliation: Department of Pathology and Laboratory Medicine, UCLA David Geffen School of Medicine, Los Angeles, CA, United States.
authors.forename: Sheeja T
authors.initials: ST
authors.lastname: Pullarkat
authors.forename: Vinod
authors.initials: V
authors.lastname: Pullarkat
authors.forename: Anand
authors.initials: A
authors.lastname: Lagoo
authors.forename: Russell
authors.initials: R
authors.lastname: Brynes
authors.forename: Lawrence M
authors.initials: LM
authors.lastname: Weiss
authors.forename: Victoria
authors.initials: V
authors.lastname: Bedell
authors.forename: Wengang
authors.initials: W
authors.lastname: Chen
authors.forename: Qin
authors.initials: Q
authors.lastname: Huang
authors.forename: Karl
authors.initials: K
authors.lastname: Gaal
authors.forename: Dennis D
authors.initials: DD
authors.lastname: Weisenburger
authors.forename: Young S
authors.initials: YS
authors.lastname: Kim
mesh_terms: D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology*; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics*; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008407:Mast Cells / Q000378:metabolism / Q000473:pathology*; D009154:Mutation / Q000235:genetics; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics; D000075142:RUNX1 Translocation Partner 1 Protein; D014178:Translocation, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: C107844:AML1-ETO fusion protein, human; D050676:Core Binding Factor Alpha 2 Subunit; D015514:Oncogene Proteins, Fusion; D000075142:RUNX1 Translocation Partner 1 Protein; D019009:Proto-Oncogene Proteins c-kit
keywords: AML with t(8 ;21); Mast cells; Mastocytosis; Shared origin of mast cells and leukemic blasts