title: Application of microbial α-amylase in industry - A review.
abstract: Amylases are one of the main enzymes used in industry. Such enzymes hydrolyze the starch molecules into polymers composed of glucose units. Amylases have potential application in a wide number of industrial processes such as food, fermentation and pharmaceutical industries. α-Amylases can be obtained from plants, animals and microorganisms. However, enzymes from fungal and bacterial sources have dominated applications in industrial sectors. The production of α-amylase is essential for conversion of starches into oligosaccharides. Starch is an important constituent of the human diet and is a major storage product of many economically important crops such as wheat, rice, maize, tapioca, and potato. Starch-converting enzymes are used in the production of maltodextrin, modified starches, or glucose and fructose syrups. A large number of microbial α-amylases has applications in different industrial sectors such as food, textile, paper and detergent industries. The production of α-amylases has generally been carried out using submerged fermentation, but solid state fermentation systems appear as a promising technology. The properties of each α-amylase such as thermostability, pH profile, pH stability, and Ca-independency are important in the development of fermentation process. This review focuses on the production of bacterial and fungal α-amylases, their distribution, structural-functional aspects, physical and chemical parameters, and the use of these enzymes in industrial applications.
authors.affiliation: Departamento de Ciências Farmacêuticas, Faculdade de Ciências da Saúde, Universidade de Brasília , Brasília, DF , Brasil.
authors.forename: Paula Monteiro
authors.initials: PM
authors.lastname: de Souza
authors.forename: Pérola
authors.initials: P
authors.lastname: de Oliveira Magalhães
keywords: bacterial and fungal amylase; enzyme production; starch; α-Amylases
references.citation: Biotechnol Appl Biochem. 2000 Apr;31(2):135-52
references.pmid: 10744959
references.citation: Biotechnol Adv. 2009 Jul-Aug;27(4):423-31
references.pmid: 19328842
references.citation: Curr Opin Biotechnol. 2002 Aug;13(4):345-51
references.pmid: 12323357
references.citation: N Biotechnol. 2009 Oct 1;26(1-2):68-74
references.pmid: 19439206
references.citation: FEMS Microbiol Lett. 2004 Feb 16;231(2):165-9
references.pmid: 14987760
references.citation: FEMS Microbiol Lett. 1998 Oct 15;167(2):139-43
references.pmid: 9867468
references.citation: Bioresour Technol. 2008 Sep;99(13):5270-95
references.pmid: 18158236
references.citation: Lett Appl Microbiol. 2002;35(1):78-84
references.pmid: 12081555
references.citation: Biochim Biophys Acta. 2004 Feb 12;1696(2):275-87
references.pmid: 14871668
references.citation: Extremophiles. 2005 Dec;9(6):487-95
references.pmid: 16075161
references.citation: Biochem Eng J. 2000 Aug 1;6(1):33-39
references.pmid: 10908866
references.citation: Folia Microbiol (Praha). 1994;39(5):392-8
references.pmid: 7729774
references.citation: J Biotechnol. 2004 Aug 5;111(3):311-8
references.pmid: 15246667
references.citation: Yeast. 2006 Sep;23(12):849-56
references.pmid: 17001624
references.citation: Bioresour Technol. 2006 Jul;97(10):1217-24
references.pmid: 16112858
references.citation: Plant Physiol Biochem. 2005 Sep;43(9):821-7
references.pmid: 16297635
references.citation: Protein Sci. 1995 Sep;4(9):1730-42
references.pmid: 8528071
references.citation: Bioresour Technol. 2007 Jan;98(1):150-7
references.pmid: 16376073
references.citation: Biochim Biophys Acta. 2000 Dec 29;1543(2):253-274
references.pmid: 11150610
references.citation: J Biosci Bioeng. 2010 Jun;109(6):531-8
references.pmid: 20471589
references.citation: Bioresour Technol. 2008 Jul;99(11):4597-602
references.pmid: 17761415
references.citation: J Microbiol Methods. 2003 Mar;52(3):353-9
references.pmid: 12531504
references.citation: Bioresour Technol. 2008 Sep;99(13):5602-9
references.pmid: 18180155
references.citation: Syst Appl Microbiol. 2005 Jul;28(5):415-20
references.pmid: 16094868
references.citation: Bioresour Technol. 2003 Nov;90(2):207-14
references.pmid: 12895565
references.citation: Bioresour Technol. 2003 Mar;87(1):57-61
references.pmid: 12733576
references.citation: Biochim Biophys Acta. 2004 Feb 12;1696(2):171-80
references.pmid: 14871658
references.citation: Appl Environ Microbiol. 1977 Oct;34(4):391-7
references.pmid: 21612
references.citation: J Biotechnol. 2002 Mar 28;94(2):137-55
references.pmid: 11796168
references.citation: Bioresour Technol. 2007 Jan;98(2):345-52
references.pmid: 16473003
references.citation: Int J Biochem Cell Biol. 2000 Nov-Dec;32(11-12):1195-204
references.pmid: 11137459
references.citation: Dig Dis Sci. 2007 Jan;52(1):1-17
references.pmid: 17205399
references.citation: Bioresour Technol. 2007 Jan;98(2):260-5
references.pmid: 16524725
references.citation: Appl Microbiol Biotechnol. 1995 Nov;43(6):1067-76
references.pmid: 8590658
references.citation: Appl Biochem Biotechnol. 2010 Apr;160(8):2401-14
references.pmid: 19763902
references.citation: Int J Food Microbiol. 2003 Jan 25;80(2):161-9
references.pmid: 12381402
references.citation: FEMS Microbiol Lett. 2000 Feb 1;183(1):67-71
references.pmid: 10650204
references.citation: Int J Biol Macromol. 2010 Apr 1;46(3):289-97
references.pmid: 20109486
references.citation: Bioresour Technol. 2008 May;99(8):3044-50
references.pmid: 17644331
references.citation: Bioresour Technol. 2001 Jan;76(2):137-41
references.pmid: 11131797
references.citation: Enzyme Microb Technol. 2000 Sep 1;27(6):406-413
references.pmid: 10938420
references.citation: Biotechnol Adv. 2005 Nov;23(7-8):471-99
references.pmid: 15919172
references.citation: J Biosci Bioeng. 2005 Aug;100(2):168-71
references.pmid: 16198259