title: Effects of polycystic ovary syndrome (PCOS), sex hormones, and obesity on circulating miRNA-21, miRNA-27b, miRNA-103, and miRNA-155 expression.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are small, noncoding RNA sequences that negatively regulate gene expression at the post-transcriptional level. miRNA-21, miRNA-27b, miRNA-103, and miRNA-155 have been associated with metabolic disorders such as obesity and diabetes, which are also associated with polycystic ovary syndrome (PCOS).
  
  
  OBJECTIVE
  We aimed to evaluate the effects of sex, sex hormones, and PCOS and their interactions with obesity on the expression in the circulation of these miRNAs.
  
  
  METHODS
  This was a case-control study.
  
  
  METHODS
  The setting was an academic hospital.
  
  
  METHODS
  We included 12 control women, 12 patients with PCOS, and 12 men selected as to have similar body mass index (BMI) and age. Six subjects per group had normal weight (BMI < 25 kg/m(2)), and six subjects per group were obese (BMI ≥ 30 kg/m(2)).
  
  
  METHODS
  Blood samples were collected early in the morning after a 12-hour fast.
  
  
  METHODS
  We measured whole blood expression of miRNA-21, miRNA-27b, miRNA-103, and miRNA-155.
  
  
  RESULTS
  Obesity significantly reduced the expression of miRNA-21, miRNA-27b, and miRNA-103. However, there was a significant interaction between obesity and the group of subjects in the expression of miRNA-21, miRNA-27b, miRNA-103, and miRNA-155 consisting of obesity reducing the expression of these miRNAs in control woman and men, but tending to increase their expression in women with PCOS. These differences paralleled those observed in serum T concentrations.
  
  
  CONCLUSIONS
  The present results suggest that miRNAs that play an important role in metabolic and immune system processes are influenced by obesity and circulating androgen concentrations.
authors.affiliation: or Héctor F. Escobar-Morreale, Department of Endocrinology and Nutrition, Hospital Universitario Ramón y Cajal, Carretera de Colmenar km 9'1, E-28034 Madrid, Spain. moramurri@gmail.com.
authors.forename: Mora
authors.initials: M
authors.lastname: Murri
authors.forename: María
authors.initials: M
authors.lastname: Insenser
authors.forename: Elena
authors.initials: E
authors.lastname: Fernández-Durán
authors.forename: José L
authors.initials: JL
authors.lastname: San-Millán
authors.forename: Héctor F
authors.initials: HF
authors.lastname: Escobar-Morreale
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression / Q000502:physiology; D012739:Gonadal Steroid Hormones / Q000097:blood; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D009765:Obesity / Q000235:genetics* / Q000378:metabolism; D011085:Polycystic Ovary Syndrome / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult
chemical_list: D012739:Gonadal Steroid Hormones; C544749:MIRN103 microRNA, human; C524942:MIRN155 microRNA, human; C524940:MIRN21 microRNA, human; C531388:MIRN27 microRNA, human; D035683:MicroRNAs