title: Subcellular localization of the pyoverdine biogenesis machinery of Pseudomonas aeruginosa: a membrane-associated "siderosome".
abstract: The peptidic siderophore pyoverdine is the primary iron uptake system of fluorescent pseudomonads, and a virulence factor in the opportunistic pathogen Pseudomonas aeruginosa. Pyoverdine biogenesis is a co-ordinate process requiring several precursor-generating enzymes and large nonribosomal peptide synthetases (NRPSs) in the cytoplasm, followed by extracytoplasmic maturation. By using cell fractionation, protein-protein interaction, and in vivo labeling assays we obtained evidence that, in P. aeruginosa, pyoverdine NRPSs assemble with precursor-generating enzymes into a membrane-bound multi-enzymatic complex, for which we propose the name "siderosome". The pyoverdine biogenetic complex represents a novel example of subcellular compartmentalization of a secondary metabolic pathway in prokaryotes.
authors.affiliation: Department of Biology and Biotechnology, Sapienza University of Rome, Rome 00185, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Imperi
authors.forename: Paolo
authors.initials: P
authors.lastname: Visca
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D003593:Cytoplasm / Q000201:enzymology / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism*; D010453:Peptide Synthases / Q000737:chemistry / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000378:metabolism; D017262:Siderophores / Q000378:metabolism*; D037521:Virulence Factors / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D009842:Oligopeptides; D017262:Siderophores; D037521:Virulence Factors; C042453:pyoverdin; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase
keywords: Bacteria; Iron; MALDI-TOF; Matrix-Assisted Laser Desorption/Ionization Time-of-Flight; NRPS; Nonribosomal peptide synthesis; Protein interaction; Siderophore; nonribosomal peptide synthetase