title: The polyketide synthase gene pks4 of Trichoderma reesei provides pigmentation and stress resistance.
abstract: Species of the fungal genus Trichoderma (Hypocreales, Ascomycota) are well-known for their production of various secondary metabolites. Nonribosomal peptides and polyketides represent a major portion of these products. In a recent phylogenomic investigation of Trichoderma polyketide synthase (PKS)-encoding genes, the pks4 from T. reesei was shown to be an orthologue of pigment-forming PKSs involved in synthesis of aurofusarin and bikaverin in Fusarium spp. In this study, we show that deletion of this gene in T. reesei results in loss of green conidial pigmentation and in pigmentation alteration of teleomorph structures. It also has an impact on conidial cell wall stability and the antagonistic abilities of T. reesei against other fungi, including formation of inhibitory metabolites. In addition, deletion of pks4 significantly influences the expression of other PKS-encoding genes of T. reesei. To our knowledge, this is the first indication that a low-molecular-weight pigment-forming PKS is involved in defense, mechanical stability, and stress resistance in fungi.
authors.affiliation: Microbiology Group, Research Area Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria.
authors.forename: Lea
authors.initials: L
authors.lastname: Atanasova
authors.forename: Benjamin P
authors.initials: BP
authors.lastname: Knox
authors.forename: Christian P
authors.initials: CP
authors.lastname: Kubicek
authors.forename: Irina S
authors.initials: IS
authors.lastname: Druzhinina
authors.forename: Scott E
authors.initials: SE
authors.lastname: Baker
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D010858:Pigmentation / Q000235:genetics*; D010860:Pigments, Biological / Q000096:biosynthesis; D048630:Polyketide Synthases / Q000235:genetics / Q000378:metabolism*; D013172:Spores, Fungal / Q000378:metabolism; D013312:Stress, Physiological; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D010860:Pigments, Biological; D048630:Polyketide Synthases
references.citation: FEMS Microbiol Lett. 2007 Oct;275(1):62-70
references.pmid: 17681008
references.citation: Eukaryot Cell. 2011 Nov;10(11):1527-35
references.pmid: 21890820
references.citation: Plant Physiol. 1996 Aug;111(4):1263-1269
references.pmid: 12226360
references.citation: FEMS Microbiol Lett. 2008 Jul;284(2):231-6
references.pmid: 18510561
references.citation: J Am Chem Soc. 2011 Oct 5;133(39):15773-85
references.pmid: 21866960
references.citation: Nucleic Acids Res. 2002 May 1;30(9):e36
references.pmid: 11972351
references.citation: Lancet. 2000 Jun 10;355(9220):2049-50
references.pmid: 10885360
references.citation: Genome Biol. 2011;12(4):R40
references.pmid: 21501500
references.citation: Microbiology (Reading). 2003 Dec;149(Pt 12):3485-3491
references.pmid: 14663081
references.citation: Curr Genet. 2009 Apr;55(2):185-98
references.pmid: 19277664
references.citation: G3 (Bethesda). 2013 Feb;3(2):369-78
references.pmid: 23390613
references.citation: Med Microbiol Immunol. 1998 Oct;187(2):79-89
references.pmid: 9832321
references.citation: Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15670-5
references.pmid: 14676319
references.citation: J Biol Chem. 2011 Mar 25;286(12):10419-28
references.pmid: 21296881
references.citation: PLoS Genet. 2012;8(8):e1002869
references.pmid: 22876203
references.citation: Microbiology (Reading). 2012 Jan;158(Pt 1):147-154
references.pmid: 22096146
references.citation: Mol Genet Genomics. 2002 Jul;267(5):636-46
references.pmid: 12172803
references.citation: Fungal Genet Biol. 2011 Apr;48(4):430-7
references.pmid: 21176790
references.citation: J Bacteriol. 1998 Jun;180(12):3031-8
references.pmid: 9620950
references.citation: Mol Microbiol. 2009 May;72(4):931-46
references.pmid: 19400779
references.citation: Med Mycol. 1999 Dec;37(6):375-89
references.pmid: 10647118
references.citation: Chem Biol. 1999 Jul;6(7):429-39
references.pmid: 10381407
references.citation: FEMS Microbiol Lett. 2012 Dec;337(1):1-9
references.pmid: 22924408
references.citation: Fungal Genet Biol. 2002 Nov;37(2):134-48
references.pmid: 12409099
references.citation: Curr Opin Microbiol. 2000 Aug;3(4):354-8
references.pmid: 10972493
references.citation: Appl Environ Microbiol. 2011 Jul;77(13):4508-19
references.pmid: 21571888
references.citation: Microbiology (Reading). 2012 Jan;158(Pt 1):35-45
references.pmid: 21998165
references.citation: Infect Immun. 2001 Sep;69(9):5760-7
references.pmid: 11500453
references.citation: Infect Immun. 2000 May;68(5):2845-53
references.pmid: 10768981
references.citation: J Chromatogr Sci. 2012 Apr;50(4):358-67
references.pmid: 22407347
references.citation: Infect Immun. 2003 Jul;71(7):4026-33
references.pmid: 12819091
references.citation: Chem Biol. 2010 May 28;17(5):483-94
references.pmid: 20534346
references.citation: Appl Environ Microbiol. 2004 May;70(5):2984-8
references.pmid: 15128560
references.citation: Infect Immun. 2002 Sep;70(9):5124-31
references.pmid: 12183562
references.citation: BMC Microbiol. 2009 Aug 24;9:177
references.pmid: 19703288
references.citation: Fungal Genet Biol. 2011 May;48(5):544-53
references.pmid: 21277986
references.citation: FEMS Microbiol Lett. 2000 Nov 1;192(1):39-44
references.pmid: 11040426
references.citation: J Chromatogr A. 2000 Oct 27;896(1-2):61-8
references.pmid: 11093640
references.citation: Nat Rev Microbiol. 2011 Sep 16;9(10):749-59
references.pmid: 21921934
references.citation: J Biol Chem. 2001 Aug 3;276(31):29292-8
references.pmid: 11350964
references.citation: Appl Microbiol Biotechnol. 2012 Feb;93(3):931-40
references.pmid: 22173481
references.citation: J Immunol Methods. 2000 Oct 20;244(1-2):69-80
references.pmid: 11033020
references.citation: Appl Environ Microbiol. 2006 Mar;72(3):2126-33
references.pmid: 16517662
references.citation: Front Microbiol. 2013 Jan 18;3:440
references.pmid: 23346079
references.citation: Int J Biochem Cell Biol. 1997 Nov;29(11):1235-9
references.pmid: 9451820
references.citation: Gene. 1987;61(2):155-64
references.pmid: 3127274
references.citation: Can J Microbiol. 1976 Feb;22(2):318-21
references.pmid: 944079
references.citation: Infect Immun. 2000 Jun;68(6):3696-703
references.pmid: 10816530
references.citation: PLoS One. 2010 Feb 12;5(2):e9191
references.pmid: 20169200
references.citation: Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):13909-14
references.pmid: 19667182
references.citation: Fungal Genet Biol. 2004 Nov;41(11):973-81
references.pmid: 15465386
references.citation: BMC Genomics. 2013 Feb 22;14:121
references.pmid: 23432824
references.citation: J Zhejiang Univ Sci B. 2010 Mar;11(3):151-68
references.pmid: 20205302
references.citation: Med Mycol. 2006 Sep;44 Suppl 1:S17-21
references.pmid: 17050415
references.citation: BMC Genomics. 2011 May 27;12:269
references.pmid: 21619626
references.citation: Fungal Genet Biol. 2005 May;42(5):420-33
references.pmid: 15809006
references.citation: Annu Rev Microbiol. 1996;50:491-512
references.pmid: 8905089
references.citation: Appl Environ Microbiol. 2005 Apr;71(4):1701-8
references.pmid: 15811992
references.citation: Can J Microbiol. 1973 Jun;19(6):725-34
references.pmid: 4712507