title: Evaluation of clay/poly (L-lactide) microcomposites as anticancer drug, 6-mercaptopurine reservoir through in vitro cytotoxicity, oxidative stress markers and in vivo pharmacokinetics.
abstract: Intercalation of 6-mercaptopurine (6-MP), an antineoplastic drug in interlayer gallery of Na(+)-clay (MMT) was further entrapped in poly (L-lactide) matrix to form microcomposite spheres (MPs) in order to reduce the cell toxicity and enhance in vitro release and pharmacokinetic proficiency. The drug-clay hybrid was fabricated via intercalation by ion-exchange method to form MPs from hybrid. In vitro drug release showed controlled pattern, fitted to kinetic models suggested controlled exchange and partial diffusion through swollen matrix of clay inter layered gallery. The in vitro efficacy of formulated composites drug was tested in Human neuroblastoma cell line (IMR32) by various cell cytotoxic and oxidative stress marker indices. In vivo pharmacokinetics suggested that the intensity of formulated drug level in plasma was within remedial borders as compared to free drug. These clay based composites therefore have great potential of becoming a new dosage form of 6-MP.
authors.affiliation: Discipline of Inorganic Materials and Catalysis, Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific and Industrial Research (CSIR), Gijubhai Badheka Marg, Bhavnagar 364 002, Gujarat, India; Institute of Science, Nirma University, Ahmedabad 382481, Gujarat, India.
authors.forename: Bhavesh D
authors.initials: BD
authors.lastname: Kevadiya
authors.forename: Shiva Shankaran
authors.initials: SS
authors.lastname: Chettiar
authors.forename: Shalini
authors.initials: S
authors.lastname: Rajkumar
authors.forename: Hari C
authors.initials: HC
authors.lastname: Bajaj
authors.forename: Kalpeshgiri A
authors.initials: KA
authors.lastname: Gosai
authors.forename: Harshad
authors.initials: H
authors.lastname: Brahmbhatt
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D001546:Bentonite / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D000077215:Clay; D004304:Dosage Forms; D004337:Drug Carriers / Q000737:chemistry*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D015122:Mercaptopurine / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D018384:Oxidative Stress / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D000538:Aluminum Silicates; D000970:Antineoplastic Agents; D004304:Dosage Forms; D004337:Drug Carriers; D011091:Polyesters; D001546:Bentonite; C033616:poly(lactide); D015122:Mercaptopurine; D000077215:Clay
keywords: 6-Mercaptopurine; Cytotoxicity; Na(+)-clay; Neuroblastoma; Oxidative stress