title: Human mitochondrial NDUFS3 protein bearing Leigh syndrome mutation is more prone to aggregation than its wild-type.
abstract: NDUFS3 is an integral subunit of the Q module of the mitochondrial respiratory Complex-I. The combined mutation (T145I + R199W) in the subunit is reported to cause optic atrophy and Leigh syndrome accompanied by severe Complex-I deficiency. In the present study, we have cloned and overexpressed the human NDUFS3 subunit and its double mutant in a soluble form in Escherichia coli. The wild-type (w-t) and mutant proteins were purified to homogeneity through a serial two-step chromatographic purification procedure of anion exchange followed by size exclusion chromatography. The integrity and purity of the purified proteins was confirmed by Western blot analysis and MALDI-TOF/TOF. The conformational transitions of the purified subunits were studied through steady state as well as time resolved fluorescence and CD spectroscopy under various denaturing conditions. The mutant protein showed altered polarity around tryptophan residues, changed quenching parameters and also noticeably altered secondary and tertiary structure compared to the w-t protein. Mutant also exhibited a higher tendency than the w-t protein for aggregation which was examined using fluorescent (Thioflavin-T) and spectroscopic (Congo red) dye binding techniques. The pH stability of the w-t and mutant proteins varied at extreme acidic pH and the molten globule like structure of w-t at pH1 was absent in case of the mutant protein. Both the w-t and mutant proteins showed multi-step thermal and Gdn-HCl induced unfolding. Thus, the results provide insight into the alterations of NDUFS3 protein structure caused by the mutations, affecting the overall integrity of the protein and finally leading to disruption of Complex-I assembly.
authors.affiliation: Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Dr Homi Bhabha Road, Pune 411008, India.
authors.forename: Tulika M
authors.initials: TM
authors.lastname: Jaokar
authors.forename: Deepak P
authors.initials: DP
authors.lastname: Patil
authors.forename: Yogesh S
authors.initials: YS
authors.lastname: Shouche
authors.forename: Sushama M
authors.initials: SM
authors.lastname: Gaikwad
authors.forename: C G
authors.initials: CG
authors.lastname: Suresh
mesh_terms: D002942:Circular Dichroism; D003001:Cloning, Molecular; D042967:Electron Transport Complex I / Q000235:genetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007888:Leigh Disease / Q000235:genetics; D008928:Mitochondria / Q000235:genetics; D009154:Mutation; D009245:NADH Dehydrogenase / Q000737:chemistry* / Q000235:genetics*; D011487:Protein Conformation / Q000187:drug effects; D011489:Protein Denaturation; D055550:Protein Stability; D020836:Protein Structure, Quaternary; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000737:chemistry
chemical_list: D014364:Tryptophan; D009245:NADH Dehydrogenase; D042967:Electron Transport Complex I; C472512:NDUFS3 protein, human
keywords: Aggregation; CD; CR; Complex-I; Congo red; Fluorescence lifetime; Leigh syndrome; NADH dehydrogenase (ubiquinone) Fe–S protein 3; NDUFS3; ORF; PBS; PVDF; RT-PCR; Th-T; Thioflavin-T; cDNA; circular dichroism; complementary DNA; open reading frame; phosphate-buffered saline; polyvinylidene fluoride; reverse transcriptase PCR; w-t; wild type