title: Bovine papillomavirus type 4 L1 gene transfection in a Drosophila S2 cell expression system: absence of L1 protein expression.
abstract: The development of a bovine papillomavirus (BPV) vaccine is an outstanding challenge. BPV protein L1 gene transfection in the Drosophila melanogaster S2 cell expression system failed to produce L1 protein notwithstanding correct L1 gene insertion. Severe genetic inbalance in the host cell line, including cytogenetic alterations, may account for the lack of protein expression.
authors.affiliation: Laboratório de Genética, Instituto Butantan , São Paulo, SP , Brasil.
authors.forename: Luiz Gustavo Bentim
authors.initials: LG
authors.lastname: Góes
authors.forename: Antonio Carlos
authors.initials: AC
authors.lastname: de Freitas
authors.forename: Oilita Pereira
authors.initials: OP
authors.lastname: Ferraz
authors.forename: Tania Tassinari
authors.initials: TT
authors.lastname: Rieger
authors.forename: José Ferreira
authors.initials: JF
authors.lastname: Dos Santos
authors.forename: Alexandre
authors.initials: A
authors.lastname: Pereira
authors.forename: Willy
authors.initials: W
authors.lastname: Beçak
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Lindsey
authors.forename: Rita
authors.initials: R
authors.lastname: de Cassia Stocco
keywords: BPV4; Bovine Papillomavirus; S2 expression cells.
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