title: miR-203 inhibits cell proliferation and migration of lung cancer cells by targeting PKCα.
abstract: PKCα (protein kinase C alpha, PRKCA) is an important protein involved in several steps of signaling pathways in lung cancer, and microRNAs (miRNAs) have also been shown to participate in lung carcinogenesis. However, it is not clear how PKCα and miRNAs are correlated in the disease. In this report, we aimed to identify novel miRNAs that target PKCα and to study their biological function. Using bioinformatics analysis, we predicted one novel candidate, miR-203, and found differential expression patterns of miR-203 and PKCα in human lung cancer tissues. Moreover, we experimentally validated miR-203 as a direct regulator of PKCα. Finally, we demonstrated that the targeting of PKCα by miR-203 played a critical role in regulating cell proliferation, apoptosis and migration in lung cancer cells. In summary, this study identifies a novel miRNA that targets PKCα and illustrates that the downregulation of PKCα by miR-203 modulates biological processes in lung cancer cells.
authors.affiliation: Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Xueliang
authors.initials: X
authors.lastname: Wang
authors.forename: Hongwei
authors.initials: H
authors.lastname: Liang
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Yan
authors.forename: Minghui
authors.initials: M
authors.lastname: Cao
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Suyang
authors.initials: S
authors.lastname: Zhang
authors.forename: Ke
authors.initials: K
authors.lastname: Zen
authors.forename: Chenyu
authors.initials: C
authors.lastname: Zhang
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
mesh_terms: D020413:3' Untranslated Regions; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D020029:Base Pairing; D001483:Base Sequence; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009367:Neoplasm Staging; D051571:Protein Kinase C-alpha / Q000235:genetics* / Q000378:metabolism; D015203:Reproducibility of Results
chemical_list: D020413:3' Untranslated Regions; C546703:MIRN203 microRNA, human; D035683:MicroRNAs; D051571:Protein Kinase C-alpha
references.citation: Toxicology. 2008 Aug 19;250(1):55-61
references.pmid: 18590793
references.citation: Cancer Res. 2007 Sep 15;67(18):8433-8
references.pmid: 17823410
references.citation: J Clin Oncol. 1998 Feb;16(2):651-7
references.pmid: 9469354
references.citation: Nat Rev Cancer. 2006 Apr;6(4):259-69
references.pmid: 16557279
references.citation: Cancer Res. 2004 Jun 1;64(11):3753-6
references.pmid: 15172979
references.citation: Oncogene. 2007 Jul 26;26(34):5017-22
references.pmid: 17297439
references.citation: Genes Dev. 2006 Mar 1;20(5):515-24
references.pmid: 16510870
references.citation: CA Cancer J Clin. 2012 Jul-Aug;62(4):220-41
references.pmid: 22700443
references.citation: Chin J Physiol. 2008 Oct 31;51(5):269-74
references.pmid: 19175182
references.citation: Clin Cancer Res. 2009 Feb 15;15(4):1177-83
references.pmid: 19228723
references.citation: J Clin Invest. 1995 Apr;95(4):1906-15
references.pmid: 7706498
references.citation: Clin Chim Acta. 2007 Jul;382(1-2):54-8
references.pmid: 17459358
references.citation: Cell. 2004 Jan 23;116(2):281-97
references.pmid: 14744438
references.citation: J Cell Biochem. 2008 Jan 1;103(1):9-20
references.pmid: 17486587
references.citation: J Cell Physiol. 2009 Apr;219(1):183-93
references.pmid: 19097143
references.citation: Nat Genet. 2005 May;37(5):495-500
references.pmid: 15806104
references.citation: Nat Med. 2011 Dec 11;18(1):74-82
references.pmid: 22157681
references.citation: Clin Cancer Res. 2011 Aug 15;17(16):5287-98
references.pmid: 21159887
references.citation: Nature. 2005 Feb 17;433(7027):769-73
references.pmid: 15685193
references.citation: Chin J Physiol. 2005 Sep 30;48(3):139-43
references.pmid: 16304840
references.citation: Cancer Res. 2003 Feb 15;63(4):780-6
references.pmid: 12591726
references.citation: FASEB J. 1995 Apr;9(7):484-96
references.pmid: 7737456
references.citation: PLoS Biol. 2004 Nov;2(11):e363
references.pmid: 15502875
references.citation: FEBS Lett. 2012 Mar 23;586(6):804-9
references.pmid: 22306317
references.citation: Nature. 2005 Jun 9;435(7043):828-33
references.pmid: 15944707
references.citation: Curr Cancer Drug Targets. 2009 Jun;9(4):572-94
references.pmid: 19519323
references.citation: Oncogene. 2006 Oct 9;25(46):6156-62
references.pmid: 17028594
references.citation: Cell Cycle. 2011 Apr 1;10(7):1121-31
references.pmid: 21368580
references.citation: Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15472-7
references.pmid: 17875987
references.citation: Curr Biol. 2007 Aug 7;17(15):1298-307
references.pmid: 17656095
references.citation: Eur J Biochem. 1997 Aug 15;248(1):1-9
references.pmid: 9310352
references.citation: Nature. 2004 Sep 16;431(7006):350-5
references.pmid: 15372042
references.citation: Cell. 2003 Dec 26;115(7):787-98
references.pmid: 14697198
references.citation: Trends Mol Med. 2006 Dec;12(12):580-7
references.pmid: 17071139
references.citation: J Exp Clin Cancer Res. 2012 Jun 19;31:58
references.pmid: 22713668
references.citation: J Biol Chem. 2011 Dec 16;286(50):43559-68
references.pmid: 22027822
references.citation: Mol Biol Cell. 2006 May;17(5):2476-87
references.pmid: 16525022
references.citation: BMC Cancer. 2009 Jun 08;9:174
references.pmid: 19500428
references.citation: Nat Rev Cancer. 2006 Nov;6(11):857-66
references.pmid: 17060945
references.citation: Cell Signal. 1998 Sep;10(8):529-42
references.pmid: 9794251
references.citation: J Biochem. 2002 Oct;132(4):509-11
references.pmid: 12359062