title: Utilization of proteinaceous materials for power generation in a mediatorless microbial fuel cell by a new electrogenic bacteria Lysinibacillus sphaericus VA5.
abstract: In this study, a bacterial strain, Lysinibacillus sphaericus which is relatively new in the vast list of biocatalysts known to produce electricity has been tested for its potential in power production. It is cited from the literature that the organism is deficient in some sugar or polysaccharide processing enzymes and thus is tested for its ability to utilize substrates mainly rich in protein components like beef extract and with successive production of electricity. The particular species has been found to generate a maximum power density of 85mW/m(2) and current density of ≈270mA/m(2) using graphite felt as electrode. The maximum Open Circuit Voltage and current has been noted as 0.7Vand 0.8mA during these operational cycles. Cyclic voltammetry studies indicate the presence of some electroactive compounds which can facilitate electron transfer from bacteria to electrode. The number of electrogens able to generate electricity in mediator free conditions are few, and the study introduces more divergence to that population. Substrate specificity and electricity generation efficacy of the strain in treating wastewater, specially rich in protein content has been reported in the study. As the species has been found to be efficient in utilizing proteinaceous material, the technique can be useful to treat specific type of wastewaters like wastewater from slaughterhouses or from meat packaging industry. Treating them in a more economical way which generates electricity as a outcome must be preferred over the conventional aerobic treatments. Emphasizing on substrate specificity, the study introduces this novel Lysinibacillus strain as a potent biocatalyst and its sustainable role in MFC application for bioenergy generation.
authors.affiliation: Department of Polymer Science and Technology, University of Calcutta, 92, A.P.C Road, Kolkata 700009, India.
authors.forename: Arpita
authors.initials: A
authors.lastname: Nandy
authors.forename: Vikash
authors.initials: V
authors.lastname: Kumar
authors.forename: Patit P
authors.initials: PP
authors.lastname: Kundu
mesh_terms: D001406:Bacillaceae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D018441:Biofilms; D057919:Biological Oxygen Demand Analysis; D001709:Biotechnology; D008855:Microscopy, Electron, Scanning; D011506:Proteins / Q000378:metabolism; D059205:Renewable Energy
chemical_list: D011506:Proteins
keywords: Electrogenic; Lysinibacillus sphaericus; Mediatorless; Microbial fuel cell; Proteinaceous substrate