title: 5-Methyldeoxycytidine enhances the substrate activity of DNA polymerase.
abstract: Here, we first demonstrated that 5-MedCTP could be incorporated into the synthetic DNA template by the exonuclease deficient Klenow fragment with a much higher efficiency than dCTP and 5-hydroxymethyl-dCTP. Further, we first conducted a comparable study of primer extension reaction using templates containing deoxycytidine (dC) or 5-methyldeoxycytidine (5-mdC) for incorporating different triphosphates. Based on our findings, 5-methyldeoxycytidine could enhance the substrate activity of the Klenow fragment (exo-) and this feature could potentially be used in DNA methylation analysis.
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Wuhan, Hubei 430072, P. R. China. xzhou@whu.edu.cn srwang@whu.edu.cn.
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.forename: Shuang
authors.initials: S
authors.lastname: Peng
authors.forename: Heng
authors.initials: H
authors.lastname: Xiao
authors.forename: Yuelin
authors.initials: Y
authors.lastname: Long
authors.forename: Boshi
authors.initials: B
authors.lastname: Fu
authors.forename: Xiaoe
authors.initials: X
authors.lastname: Zhang
authors.forename: Shan
authors.initials: S
authors.lastname: Guo
authors.forename: Shaoru
authors.initials: S
authors.lastname: Wang
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
authors.forename: Songmei
authors.initials: S
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
mesh_terms: D004247:DNA / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D020536:Enzyme Activators / Q000494:pharmacology; D013379:Substrate Specificity / Q000187:drug effects
chemical_list: D020536:Enzyme Activators; D003841:Deoxycytidine; D004247:DNA; C016569:5-methyldeoxycytidine; D004259:DNA-Directed DNA Polymerase