title: Antimicrobial activity of some essential oils against microorganisms deteriorating fruit juices.
abstract: Seventeen microbial species including 10 fungal taxa, two yeasts and five bacteria, were isolated from freshly prepared orange, guava and banana juices kept in open bottles at room temperature for 7 days. Eight different essential oils, from local herbs, were tested for their antimicrobial activity against these test organisms. The essential oils of Cymbopogon citratus, Ocimum basilicum and Origanum majorana were found to be highly effective against these microorganisms. Aspergillus niger, A. flavus and Saccharomyces cerevisiae, the most prevalent microorganisms in juice, showed the highest resistance against these essential oils. GC-MS analysis showed that while e-citral, a'-myrcene, and z-citral represent the major components (75.1%) of the essential oil of Cymbopogon citratus; bezynen,1-methyl-4-(2-propenyl), 1,8-cineole and trans-a'-bisabolene were the main components (90.6%) of Ocimum basilicum; whereas 3-cyclohexen-1-01,4-methyl-1(1-methylethyl)-(CAS), c-terpinene and trans-caryophyllene represent the major components (65.1%) of Origanum majorana. These three essential oils were introduced into juices by two techniques namely, fumigation and direct contact. The former technique showed more fungicidal effect than the latter one against A. flavus, A. niger, and S. cerevisiae. The essential oil of Cymbopogon citratus by comparison to other test oils showed the strongest effect against these fungi with a minimum inhibitory concentration of 1.5 µl/ml medium and a sublethal concentration of 1.0 µl/ml. The antimicrobial activity of this oil is thermostable at 121℃ for 30 min.
authors.affiliation: Botany Department, Faculty of Science, Zagazig University, Sharkia Governorate, Egypt.
authors.forename: G A
authors.initials: GA
authors.lastname: Helal
authors.forename: M M
authors.initials: MM
authors.lastname: Sarhan
authors.forename: A N K
authors.initials: AN
authors.lastname: Abu Shahla
authors.forename: E K
authors.initials: EK
authors.lastname: Abou El-Khair
keywords: Antimicrobial activity; Bacteria; Essential oils; Fungi; Yeast
references.citation: Planta Med. 2000 Aug;66(6):556-60
references.pmid: 10985085
references.citation: Appl Environ Microbiol. 1994 Apr;60(4):1101-5
references.pmid: 8017906
references.citation: J Basic Microbiol. 2006;46(6):456-69
references.pmid: 17139611
references.citation: J Agric Food Chem. 2003 Jan 1;51(1):76-81
references.pmid: 12502388
references.citation: J Appl Microbiol. 2001 Feb;90(2):279-84
references.pmid: 11168731
references.citation: Oral Microbiol Immunol. 2005 Apr;20(2):101-5
references.pmid: 15720570
references.citation: J Basic Microbiol. 2006;46(5):375-86
references.pmid: 17009293
references.citation: J Agric Food Chem. 2000 Jun;48(6):2576-81
references.pmid: 10888587
references.citation: Lett Appl Microbiol. 1997 Apr;24(4):269-75
references.pmid: 9134774
references.citation: Int J Food Microbiol. 2000 Sep 25;60(2-3):219-29
references.pmid: 11016611
references.citation: J Ethnopharmacol. 2001 Jul;76(2):183-6
references.pmid: 11390134
references.citation: Mycoses. 2000;43(1-2):17-23
references.pmid: 10838841
references.citation: Lett Appl Microbiol. 1998 Oct;27(4):207-10
references.pmid: 9812396
references.citation: Int J Food Microbiol. 2002 Mar 25;74(1-2):101-9
references.pmid: 11929164
references.citation: Microbios. 1996;86(349):237-46
references.pmid: 8893526
references.citation: Protoplasma. 2005 Apr;225(1-2):57-65
references.pmid: 15868213
references.citation: Mycopathologia. 1994 Dec;128(3):151-3
references.pmid: 7739729
references.citation: J Basic Microbiol. 2007 Feb;47(1):5-15
references.pmid: 17304618
references.citation: Microbiol Immunol. 2001;45(3):201-8
references.pmid: 11345529
references.citation: J Appl Microbiol. 1999 Jun;86(6):985-90
references.pmid: 10438227
references.citation: J Appl Microbiol. 2001 Sep;91(3):492-7
references.pmid: 11556915
references.citation: Lett Appl Microbiol. 2002;34(1):27-31
references.pmid: 11849488
references.citation: J Agric Food Chem. 2002 Nov 6;50(23):6688-91
references.pmid: 12405762
references.citation: Planta Med. 1987 Oct;53(5):395-8
references.pmid: 3324126
references.citation: J Agric Food Chem. 2003 Jan 1;51(1):63-7
references.pmid: 12502386
references.citation: J Appl Bacteriol. 1995 Mar;78(3):264-9
references.pmid: 7730203
references.citation: Z Naturforsch C J Biosci. 2002 May-Jun;57(5-6):478-82
references.pmid: 12132688
references.citation: J Appl Microbiol. 2003;94(4):665-74
references.pmid: 12631202
references.citation: J Appl Microbiol. 2003;94(5):893-9
references.pmid: 12694455
references.citation: J Appl Microbiol. 1998 Mar;84(3):368-76
references.pmid: 9721641
references.citation: J Agric Food Chem. 2004 Sep 22;52(19):6042-8
references.pmid: 15366861