title: Integrative taxonomy identifies a new species of Phyllodistomum (Digenea: Gorgoderidae) from the twospot livebearer, Heterandria bimaculata (Teleostei: Poeciliidae), in Central Veracruz, Mexico.
abstract: Phyllodistomum inecoli n. sp. is described from the twospot livebearer, Heterandria bimaculata (Teleostei: Poeciliidae), collected in the Río La Antigua basin, Veracruz, Mexico. The new species is described and characterised by using a combination of morphology, scanning electron microscopy, and sequences of nuclear and mitochondrial genes. Diagnostic characters of the new species of Phyllodistomum include a genital pore opening at the level of the caecal bifurcation; oval vitellarium, situated just posterior to the ventral sucker and not extended laterally and anterior extracaecal uterine loops variable in extension (reaching the anterior, median or posterior margin of the ventral sucker). P. inecoli n. sp. most closely resembles P. brevicecum, a species described as a parasite of the central mudminnow, Umbra limi, in other parts of North America; however, the genital pore in P. brevicecum is situated between the caecal bifurcation and the ventral sucker, the ovary is larger, the vitellarium is lobed and extended laterally and the anterior portion of the uterus extends to the posterior margin of the ventral sucker. Comparison of about 1,500–2,200 nucleotides of cox1 and 28S rDNA and ITS1 strongly supports the status of P. inecoli as a new species. Bayesian inference analysis of combined datasets of 28S rDNA and cox1 sequences showed that P. inecoli n. sp. and the other species found in freshwater fishes of Mexico, including the species complex of P. lacustri, are not sister species. Phylogenetic analysis based on 28S rDNA sequences of several gorgoderid taxa revealed the close relationship of P. inecoli n. sp. with several species of Phyllodistomum, Gorgodera and Gorgoderina with cystocercous cercariae developing in sphaeriid bivalves. Dot-plot analysis of ITS1 sequences of P. inecoli n. sp. revealed the presence of eight repetitive elements with different length, which together represent almost half the length of ITS1.
authors.forename: Ulises
authors.initials: U
authors.lastname: Razo-Mendivil
authors.forename: Gerardo
authors.initials: G
authors.lastname: Pérez-Ponce de León
authors.forename: Miguel
authors.initials: M
authors.lastname: Rubio-Godoy
mesh_terms: D000818:Animals; D001499:Bayes Theorem; D058487:Cercaria; D003532:Cyprinodontiformes / Q000469:parasitology*; D018509:DNA, Helminth / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005618:Fresh Water; D008800:Mexico; D010802:Phylogeny; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D016415:Sequence Alignment; D014200:Trematoda / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary
chemical_list: D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer; D012339:RNA, Ribosomal, 28S
references.citation: Nat Methods. 2012 Jul 30;9(8):772
references.pmid: 22847109
references.citation: Int J Parasitol. 2002 Mar;32(3):321-8
references.pmid: 11835972
references.citation: Parasitol Res. 2013 Aug;112(8):3063-74
references.pmid: 23760874
references.citation: Parasitology. 1998 Apr;116 ( Pt 4):311-7
references.pmid: 9585933
references.citation: Syst Biol. 2012 May;61(3):539-42
references.pmid: 22357727
references.citation: Int J Parasitol. 2010 Mar 15;40(4):471-86
references.pmid: 19853608
references.citation: J Parasitol. 2013 Feb;99(1):93-101
references.pmid: 22924927
references.citation: Mol Phylogenet Evol. 1999 Jun;12(1):67-73
references.pmid: 10222162
references.citation: Mol Phylogenet Evol. 2011 May;59(2):331-41
references.pmid: 21356320
references.citation: Mol Phylogenet Evol. 2007 Jun;43(3):986-98
references.pmid: 17185005
references.citation: Eur J Biochem. 1970 Sep;16(1):1-11
references.pmid: 5456129
references.citation: Mol Biol Evol. 2001 Apr;18(4):648-60
references.pmid: 11264417
references.citation: J Parasitol. 2013 Jun;99(3):501-8
references.pmid: 23327447
references.citation: Parasitol Res. 2005 Nov;97(5):420-3
references.pmid: 16151738
references.citation: Syst Biol. 2005 Oct;54(5):844-51
references.pmid: 16243769
references.citation: Parasitology. 2002 Mar;124(Pt 3):307-13
references.pmid: 11922432
references.citation: Syst Biol. 2003 Oct;52(5):696-704
references.pmid: 14530136
references.citation: J Parasitol. 2010 Feb;96(1):236-44
references.pmid: 19803544
references.citation: Mol Phylogenet Evol. 1995 Jun;4(2):103-9
references.pmid: 7663756
references.citation: Comput Appl Biosci. 1996 Dec;12(6):543-8
references.pmid: 9021275
references.citation: J Parasitol. 2011 Aug;97(4):661-70
references.pmid: 21506841
references.citation: J Parasitol. 2013 Dec;99(6):1114-21
references.pmid: 23915018
references.citation: Annu Rev Entomol. 2010;55:421-38
references.pmid: 19737081
references.citation: J Parasitol. 2003 Apr;89(2):346-50
references.pmid: 12760652
references.citation: Nucleic Acids Res. 2004 Mar 19;32(5):1792-7
references.pmid: 15034147