title: Functional conservation between mammalian MGRN1 and plant LOG2 ubiquitin ligases.
abstract: Plant LOSS OF GDU 2 (LOG2) and Mammalian Mahogunin Ring Finger 1 (MGRN1) proteins are RING-type E3 ligases sharing similarity N-terminal to the RING domain. Deletion of this region disrupts the interaction of LOG2 with the plant membrane protein GLUTAMINE DUMPER1 (GDU1). Phylogenetic analysis identified two clades of LOG2/MGRN1-like proteins in vertebrates and plants. The ability of MGRN1 to functionally replace LOG2 was tested. MGRN1 ubiquitylates GDU1 in vitro and can partially substitute for LOG2 in the plant, partially restoring amino acid resistance to a GDU1-myc over-expression, log2-2 background. Altogether, these results suggest a conserved function for the N-terminal domain in evolution.
authors.affiliation: Department of Molecular and Cellular Biology, UC Davis, Davis, CA 95616, United States; UC Davis Biochemistry, Molecular, Cellular, Developmental Biology Graduate Group, United States.
authors.forename: Damian D
authors.initials: DD
authors.lastname: Guerra
authors.forename: Réjane
authors.initials: R
authors.lastname: Pratelli
authors.forename: Edward
authors.initials: E
authors.lastname: Kraft
authors.forename: Judy
authors.initials: J
authors.lastname: Callis
authors.forename: Guillaume
authors.initials: G
authors.lastname: Pilot
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008322:Mammals; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D025801:Ubiquitin / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics* / Q000378:metabolism; D054875:Ubiquitination
chemical_list: C492757:GDU1 protein, Arabidopsis; D008565:Membrane Proteins; D010940:Plant Proteins; D025801:Ubiquitin; D044767:Ubiquitin-Protein Ligases
keywords: DAR2; DOMAIN ASSOCIATED WITH RING 2; ESCRT; GDU; GDU1; GLUTAMINE DUMPER; GST; LOG2; LOSS OF GDU 2; MCR; MGRN1; Mahogunin; Membrane trafficking; RING; RNF; Ubiquitin ligase; VIMAG; Val-Ile-Met-Ala-Gly; cGDU1; cytosolic domain of GDU1; endosomal sorting complex required for transport; glutathione-S-transferase; mahogunin ring finger 1; melancortin receptors; really interesting new gene; ring finger
references.citation: PLoS One. 2012;7(6):e38591
references.pmid: 22723868
references.citation: Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W526-31
references.pmid: 15215442
references.citation: Plant J. 1998 Dec;16(6):735-43
references.pmid: 10069079
references.citation: J Exp Bot. 2006;57(7):1579-89
references.pmid: 16595578
references.citation: Mol Biol Cell. 2007 Apr;18(4):1129-42
references.pmid: 17229889
references.citation: Plant Physiol. 2010 Feb;152(2):762-73
references.pmid: 20018597
references.citation: J Biol Chem. 2009 Nov 13;284(46):31714-25
references.pmid: 19737927
references.citation: BMC Genomics. 2004 Jun 17;5(1):37
references.pmid: 15202951
references.citation: Plant Physiol. 2013 Jul;162(3):1733-49
references.pmid: 23696092
references.citation: Mol Biol Evol. 2011 Oct;28(10):2731-9
references.pmid: 21546353
references.citation: Genetics. 1997 Aug;146(4):1407-15
references.pmid: 9258683
references.citation: Plant Mol Biol. 2011 May;76(1-2):85-96
references.pmid: 21442383
references.citation: Plant Cell. 2004 Jul;16(7):1827-40
references.pmid: 15208395
references.citation: Plant Physiol. 2012 Apr;158(4):1628-42
references.pmid: 22291198
references.citation: Plant Physiol. 2012 Sep;160(1):56-64
references.pmid: 22730427
references.citation: Proc Int Conf Intell Syst Mol Biol. 1994;2:28-36
references.pmid: 7584402
references.citation: Annu Rev Biochem. 2009;78:399-434
references.pmid: 19489725
references.citation: Biochim Biophys Acta. 1999 Aug 12;1451(1):1-16
references.pmid: 10446384
references.citation: Endocrinology. 2011 Nov;152(11):4224-31
references.pmid: 21862608
references.citation: Pigment Cell Melanoma Res. 2013 Mar;26(2):263-8
references.pmid: 23253940
references.citation: Proteomics. 2005 Jan;5(1):35-45
references.pmid: 15602774
references.citation: Proteomics. 2010 May;10(9):1780-93
references.pmid: 20213681
references.citation: J Biosci Bioeng. 2007 Jul;104(1):34-41
references.pmid: 17697981
references.citation: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references.pmid: 9254694
references.citation: Biochem Soc Trans. 2009 Oct;37(Pt 5):937-53
references.pmid: 19754430
references.citation: Cell Signal. 1997 Jan;9(1):15-35
references.pmid: 9067626
references.citation: J Biol Chem. 2011 May 27;286(21):18914-29
references.pmid: 21460229
references.citation: Eur J Pharmacol. 2011 Jun 11;660(1):125-30
references.pmid: 21208602
references.citation: Biochim Biophys Acta. 2009 Oct;1792(10):1027-35
references.pmid: 19703557