title: [Clinicopathological significance of abnormal metabolism of polyunsaturated fatty acids in colorectal cancer tissue].
abstract: OBJECTIVE
  To explore the relationship between different tissue levels of polyunsaturated fatty acid (PUFA) and the clinicopathologic parameters in colorectal carcinoma (CRC) and evaluate their prognostic significance.
  
  
  METHODS
  Fresh frozen malignant tissue was obtained from 82 colorectal cancer patients at PLA general Hospital from December 2010 to March 2011. The immunohistochemical results were obtained for vascular endothelial growth factor (VEGF), P53 and Ki-67. The relationship between the PUFA level and such clinicopathological profiles as age, gender, location, differentiation degree, TNM (tumor, node and metastasis) stage, VEGF, Ki-67 and P53 was analyzed.
  
  
  RESULTS
  Tissue level of arachidonic acid (AA) in patients aged over 60 years (n = 44) was significantly lower than those under 60 years (n = 38) (0.12% ± 0.06% vs 0.17% ± 0.09%, P = 0.045). In patients with tumor size < 5 cm (n = 42), tissue level of EPA was significantly higher (0.29% ± 0.13% vs 0.20% ± 0.14%, P = 0.030) while ω-6/ω-3 PUFA lower (10.8 ± 2.6 vs 13.2 ± 6.4, P = 0.031). Significant statistical difference existed in tissue level of LA, AA/ω-3 PUFA in different differentiation degrees(P = 0.013, 0.027). Tissue level of linoleic acid(LA) in poorly differentiated tumor was the highest (19.9% ± 6.3%) while AA/ω-3 PUFA the lowest (4.1 ± 2.0, P < 0.05). Tissue level of LA was higher in VEGF-positive tumors than those in VEGF-negative counterparts(16.2% ± 3.7% vs 13.9% ± 2.7%, P = 0.009) while the ratios AA/ω-3 PUFA, AA/ω-6 PUFA, AA/LA in VEGF-positive tumors were lower than those in VEGF-negative counterparts (5.0 ± 1.8 vs 6.7 ± 3.3, 0.30 ± 0.09 vs 0.34 ± 0.09, 0.50 ± 0.21 vs 0.61 ± 0.21, P = 0.004, 0.038, 0.030) . In Ki-67 negative LA was highest (22.5% ± 10.1%, P = 0.048). No significant differences existed in the level of PUFA among the gender, the clinicopathological stage, lymph node metastasis and groups with differential expressions of P53 (all P > 0.05).
  
  
  CONCLUSIONS
  The tissue levels of PUFA are somewhat correlated with the clinicopathologic parameters of CRC. And the prognosis of CRC may be evaluated through the test of PUFA.
authors.affiliation: Department of General Surgery, Chinese People's Liberation Army General Hospital, Beijing, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.forename: Wen
authors.initials: W
authors.lastname: Tian
authors.forename: Po
authors.initials: P
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016718:Arachidonic Acid / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D015525:Fatty Acids, Omega-3 / Q000378:metabolism*; D043371:Fatty Acids, Omega-6 / Q000378:metabolism*; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D019394:Ki-67 Antigen; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D016718:Arachidonic Acid