title: The mitochondrial protective mechanism of olfactory ensheathing cells conditioned medium protects against H2O2-induced injury in astrocytes.
abstract: Our previous studies showed that olfactory ensheathing cells conditioned medium (OECCM), has a positive effect against apoptosis in ASTs and prevents the morphology changes in the, mitochondria, but the accurate mechanism is still unknown. In this study, we examined the mitochondrial mechanism of OECCM which may protect against H2O2-induced injury in ASTs. It was, found that OECCM could protect ASTs from the injury of 500μmol/L H2O2, decrease the intracellular, ROS and Ca(2+) level, as well inhibit apoptosis and the expression of cleaved caspase-3. Further, investigations showed that OECCM could increase both the mitochondrial membrane potential and the, ATP level, as well enhance the cell respiratory function. In summary, OECCM could protect ASTs from, damages induced by H2O2. Its mechanism may be related to the decrease of ROS generation and the, overloading of Ca(2+), then stabilization of the mitochondrial function.
authors.affiliation: Department of Orthopedics, The Third Affiliated Hospital of Suzhou University, Changzhou 213003, PR China.
authors.forename: Jinbo
authors.initials: J
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Qiu
authors.forename: Yuyun
authors.initials: Y
authors.lastname: Xiong
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
mesh_terms: D000818:Animals; D017209:Apoptosis; D001253:Astrocytes / Q000166:cytology* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002470:Cell Survival; D017077:Culture Media, Conditioned / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology*; D008928:Mitochondria / Q000378:metabolism*; D009830:Olfactory Bulb / Q000166:cytology / Q000378:metabolism*; D009832:Olfactory Nerve / Q000166:cytology / Q000378:metabolism*; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D002118:Calcium
keywords: Apoptosis; Astrocytes (ASTs); H(2)O(2); Mitochondria; Olfactory ensheathing cells conditioned medium (OECCM)