title: Tracking the spectroscopic and chromatographic changes of algal derived organic matter in a microbial fuel cell.
abstract: Changes in the characteristics of algae-derived organic matter (AOM) were examined upon the operation of a microbial fuel cell (MFC) using multiple analytical methods. Temporal variations in the UV absorption and fluorescence excitation-emission matrix of the AOM revealed that less condensed humic-like components and large-sized protein-like fluorescent compounds were preferentially decomposed over the period of electricity generation. They also showed that low UV-absorbing extracellular organic matters (EOM) were produced at the end of the operation. SEC chromatograms demonstrated that smaller-sized UV-absorbing components were initially decomposed, followed by the net production of EOM with an intermediate molecular weight. Fourier transform infrared (FT-IR) spectra showed that proteins and polysaccharides were the two most dominant structures of the AOM in the MFC. Two-dimensional correlation spectroscopy combined with FT-IR provided additional valuable information on the sequential changes of the AOM, which occurred in the order of proteins → acidic functional groups → polysaccharides → amino acids/proteins.
authors.affiliation: Department of Environment and Energy, Sejong University, Seoul, 143-747, South Korea. jinhur@sejong.edu.
authors.forename: Jin
authors.initials: J
authors.lastname: Hur
authors.affiliation: Department of Environment and Energy, Sejong University, Seoul, 143-747, South Korea.
authors.forename: Bo-Mi
authors.initials: BM
authors.lastname: Lee
authors.affiliation: Department of Environmental Science and Engineering, Kyung Hee University, Youngin, 446-701, South Korea.
authors.forename: Kwang-Soon
authors.initials: KS
authors.lastname: Choi
authors.affiliation: Department of Environmental Science and Engineering, Kyung Hee University, Youngin, 446-701, South Korea. bkmin@khu.ac.kr.
authors.forename: Booki
authors.initials: B
authors.lastname: Min
mesh_terms: D001674:Bioelectric Energy Sources; D004560:Electricity; D058086:Microalgae; D008970:Molecular Weight; D011506:Proteins; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D011506:Proteins
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