title: MicroRNA hsa-miR-125a-3p activates p53 and induces apoptosis in lung cancer cells.
abstract: The mature microRNA hsa-miR-125a-3p is derived from the 3' end of pre-miR-125a. Here, we reported that hsa-miR-125a-3p suppressed proliferation and induced apoptosis in A549 cells. In addition, wild-type p53 mRNA and protein expression was increased by hsa-miR-125a-3p over-expression. Moreover, blocking wild-type p53 attenuated the effect of hsa-miR-125a-3p on apoptosis but could not restore completely. In p53-deficient cell line H1299, hsa-miR-125a-3p still induced apoptosis. Taken together, these data suggest that hsa-miR-125a-3p induces apoptosis not only via the p53 pathway in human lung cancer cells. These results provide new insight into the roles of the miR-125a family in lung cancer.
authors.affiliation: Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences, China Medical University , Shenyang, Liaoning , China,1.
authors.forename: Lili
authors.initials: L
authors.lastname: Jiang
authors.forename: Jihong
authors.initials: J
authors.lastname: Chang
authors.forename: Qingfu
authors.initials: Q
authors.lastname: Zhang
authors.forename: Limei
authors.initials: L
authors.lastname: Sun
authors.forename: Xueshan
authors.initials: X
authors.lastname: Qiu
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004789:Enzyme Activation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008168:Lung / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53