title: Association between polymorphism of beta3-adrenoceptor gene and overactive bladder.
abstract: OBJECTIVE
  In human urinary bladder, beta3-ARs play an important role in promoting detrusor relaxation during the storage phase of the micturition cycle. The present study investigated whether a Trp64Arg polymorphism of the gene encoding the beta3-AR is associated with overactive bladder (OAB) syndrome.
  
  
  METHODS
  This study involved 100 women with OAB and 101 healthy control women without OAB. Hair root samples were obtained from all subjects and used for beta3-AR gene analysis. Polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis was performed to analyze a polymorphism in the gene of Trp64Arg.
  
  
  RESULTS
  The overall frequency of the 64Arg variant (heterozygous plus homozygous) in OAB patients was 47% and significantly higher than the frequency of 22.8% found in non-OAB control women. Within OAB patients, numbers of micturitions per day, urgency episodes per day, and urgency incontinence episodes per day in the 64Arg variant carriers were not significantly different from those in the normal gene carriers.
  
  
  CONCLUSIONS
  This study shows that the Trp64Arg polymorphism in the beta3-AR gene is weakly but significantly associated with OAB syndrome.
authors.affiliation: Ooizumihonda Clinic, Date City, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Honda
authors.forename: Osamu
authors.initials: O
authors.lastname: Yamaguchi
authors.forename: Masanori
authors.initials: M
authors.lastname: Nomiya
authors.forename: Keiichi
authors.initials: K
authors.lastname: Shishido
authors.forename: Kei
authors.initials: K
authors.lastname: Ishibashi
authors.forename: Norio
authors.initials: N
authors.lastname: Takahashi
authors.forename: Ken
authors.initials: K
authors.lastname: Aikawa
mesh_terms: D000328:Adult; D000368:Aged; D004247:DNA / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D022702:Receptors, Adrenergic, beta-3 / Q000235:genetics*; D053201:Urinary Bladder, Overactive / Q000235:genetics*
chemical_list: D022702:Receptors, Adrenergic, beta-3; D004247:DNA
keywords: beta3-adrenoceptor; overactive bladder; polymorphism