title: Detection of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil.
abstract: To determine the presence of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil, 80 animals slaughtered in the public slaughterhouse of Patos city were used. Before slaughter, blood samples were collected by jugular venopuncture from each animal, and after slaughter, testicles, epidydimus and uterus were aseptically collected. For the serological diagnosis of B. ovis and B. abortus infections, the agar gel immunodiffusion (AGID) and Rose Bengal (RBT) tests were carried out, respectively. In addition, microbiological culture and polymerase chain reaction (PCR) were performed on testicle, epidydimus and uterus samples. Six animals (7.5%) tested positive for the presence of B. ovis antibodies and all animals tested negative for the presence of B. abortus antibodies. One AGID-positive animal tested positive at uterine swab culture. PCR was able to amplify DNA of Brucella spp. from the pool of testicle, epidydimus and uterus samples from AGID-positive animals. This is the first report of isolation and detection of B. ovis DNA by PCR in ovine from the Northeast region of Brazil.
authors.affiliation: Unidade Acadêmica de Medicina Veterinária, Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande , Patos, PB , Brasil.
authors.forename: Clebert José
authors.initials: CJ
authors.lastname: Alves
authors.forename: Salomão Moreira
authors.initials: SM
authors.lastname: de Figueiredo
authors.forename: Sérgio Santos
authors.initials: SS
authors.lastname: de Azevedo
authors.forename: Inácio José
authors.initials: IJ
authors.lastname: Clementino
authors.forename: Lara Borges
authors.initials: LB
authors.lastname: Keid
authors.forename: Sílvio Arruda
authors.initials: SA
authors.lastname: Vasconcellos
authors.forename: Carolina
authors.initials: C
authors.lastname: de Sousa Américo Batista
authors.forename: Vivianne Cambuí Mesquita
authors.initials: VC
authors.lastname: Rocha
authors.forename: Severino Silvano
authors.initials: SS
authors.lastname: Higino
keywords: Brucella ovis; PCR; Paraíba State; isolation; ovine
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