title: Quinoline-based fluorescent probe for ratiometric detection of lysosomal pH.
abstract: A new pH-responsive fluorescent probe has been reported based on protonation-activable resonance charge transfer. In aqueous solution, probe PQ-Lyso exhibits ratiometric detection of pH changes with a large hypsochromic shift of 76 nm and remarkable changes in the fluorescence intensity ratio (R = F(494 nm)/F(570 nm), R/R(0) = 105). Furthermore, PQ-Lyso, which is localized to lysosomes in living cells, can calibrate lysosomal pH using fluorescence ratiometry.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China, and College of Chemistry, Beijing Normal University , Beijing 100875, P. R. China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Li
authors.forename: Dongjian
authors.initials: D
authors.lastname: Zhu
authors.forename: Lin
authors.initials: L
authors.lastname: Xue
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000737:chemistry* / Q000378:metabolism; D011804:Quinolines / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D011804:Quinolines