title: Rapid and simultaneous detection of the chlorhexidine and mupirocin resistance genes qacA/B and mupA in clinical isolates of methicillin-resistant Staphylococcus aureus.
abstract: We describe a real-time PCR-based assay capable of simultaneously detecting femA (Staphylococcus aureus-specific), mecA (methicillin resistance), qacA/B (chlorhexidine tolerance), and mupA (high-level mupirocin resistance) from bacterial cells in less than 90 minutes. The assay was validated with 1968 clinical MRSA submitted to a surveillance network.
authors.affiliation: Multidrug-resistant organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, MD, USA. Electronic address: patrick.t.mcgann4@mail.mil.
authors.forename: Patrick
authors.initials: P
authors.lastname: Mc Gann
authors.forename: Michael
authors.initials: M
authors.lastname: Milillo
authors.forename: Yoon I
authors.initials: YI
authors.lastname: Kwak
authors.forename: Reyes
authors.initials: R
authors.lastname: Quintero
authors.forename: Paige E
authors.initials: PE
authors.lastname: Waterman
authors.forename: Emil
authors.initials: E
authors.lastname: Lesho
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D002710:Chlorhexidine / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D006801:Humans; D026901:Membrane Transport Proteins / Q000235:genetics*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification; D016712:Mupirocin / Q000494:pharmacology; D009687:Nuclear Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013203:Staphylococcal Infections / Q000382:microbiology*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C096686:FemA protein, Bacteria; D026901:Membrane Transport Proteins; D009687:Nuclear Proteins; C099272:QacB protein, Staphylococcus aureus; C480380:mupA protein, Staphylococcus aureus; C068498:qacA protein, Staphylococcus aureus; D016712:Mupirocin; D002710:Chlorhexidine
keywords: Chlorhexidine; MRSA; MupA; Mupirocin; QacA