title: Frequent promoter methylation of CDH1 in non-neoplastic mucosa of sporadic diffuse gastric cancer.
abstract: OBJECTIVE
  To identify promoter methylation as a major silencing mechanism in potential precursor lesions of sporadic diffuse gastric cancer (DGC), we investigated promoter methylation of CDH1 (E-Cadherin gene) in a series of DGCs and matched normal mucosa.
  
  
  METHODS
  The extent of CDH1 gene promoter methylation was explored using methylation-specific polymerase chain reaction (MSP) and pyrosequencing (PS) in 72 DGCs with a matched pair of normal mucosa.
  
  
  RESULTS
  MSP and PS revealed CDH1 promoter methylation in 73.6% (53/72) and 77.8% (56/72) of DGC samples, respectively. PS detected CDH1 methylation in 70.8% (51/72) and 72.2% (52/72) of matched normal mucosa from adjacent and remote foci, respectively. In comparison, CDH1 promoter methylation was detected in 12/24 normal gastric mucosa samples without DGC, and this difference was statistically significant (p=0.016).
  
  
  CONCLUSIONS
  The current study showed that the majority of DGC cases demonstrated concurrent CDH1 promoter methylation in tumor tissues and in non-neoplastic mucosa.
authors.affiliation: Department of Pathology, Chonnam National University Medical School, #8, Hak-dong, Dong-gu, Gwangju, 501-757, Republic of Korea. jhlee@jnu.ac.kr.
authors.forename: Kyung Hwa
authors.initials: KH
authors.lastname: Lee
authors.forename: David
authors.initials: D
authors.lastname: Hwang
authors.forename: Ki Young
authors.initials: KY
authors.lastname: Kang
authors.forename: Soong
authors.initials: S
authors.lastname: Lee
authors.forename: Dong Yi
authors.initials: DY
authors.lastname: Kim
authors.forename: Young Eun
authors.initials: YE
authors.lastname: Joo
authors.forename: Jae Hyuk
authors.initials: JH
authors.lastname: Lee
mesh_terms: D015703:Antigens, CD; D001483:Base Sequence; D015820:Cadherins / Q000235:genetics*; D019175:DNA Methylation; D017931:DNA Primers; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D017931:DNA Primers
keywords: Methylation; diffuse gastric cancer; human CDH1 protein; methylation-specific polymerase chain reaction; pyrosequencing; stomach neoplasm