title: [Telomere length and gene expression of shelterin in CD3(+) T cell of severe aplastic anemia patients].
abstract: OBJECTIVE
  To explore the changes in telomere length and gene expression of complex shelterin (composed of 6 core components: TRF1, TRF2, POT1, TIN2, TPP1 and RAP1) in severe aplastic anemia (SAA).
  
  
  METHODS
  Bone marrow samples were obtained from 20 SAA patients and 10 normal controls. CD3(+)T cells were sorted by immunomagnetic separation. Telomere length was tested by Southern blot and the gene expressions of TRF1, TRF2, POT1, TIN2, TPP1 and RAP1 were detected by reverse transcription-PCR(RT-PCR).
  
  
  RESULTS
  Telomeres of CD3(+)T cells were found significantly shorter in SAA untreated ((4.4 ± 1.1) kb, n = 9) and recovering groups((5.8 ± 1.0) kb, n = 11) than control group ((9.2 ± 3.3) kb, P < 0.05). Telomere length of CD3(+)T cells shortened with TH/S decreasing (r = 0.564, P = 0.029). The mRNA expression of POT1 decreased in untreated SAA patients (0.16(0.02-0.29)) and over-expressed in recovering patients (1.17(0.82-1.86), P < 0.05). The mRNA expression of RAP1 was significantly higher in untreated patients (4.14 (1.93-6.92)) than that in recovering group (0.87 (0.30-1.73) ) and controls (0.62 (0.45-4.07) , both P < 0.05).
  
  
  CONCLUSIONS
  Changes in telomere length and shelterin gene expression occur in CD3(+)T cells of SAA patients and may be correlated with disease severity.
authors.affiliation: Department of Hematology & Oncology, General Hospital, Tianjin Medical University, Tianjin, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.forename: Rong
authors.initials: R
authors.lastname: Fu
authors.forename: Hua-quan
authors.initials: HQ
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Qu
authors.forename: Er-bao
authors.initials: EB
authors.lastname: Ruan
authors.forename: Guo-jin
authors.initials: GJ
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Yu-hong
authors.initials: YH
authors.lastname: Wu
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Wang
authors.forename: Jia
authors.initials: J
authors.lastname: Song
authors.forename: Li-min
authors.initials: LM
authors.lastname: Xing
authors.forename: Jing
authors.initials: J
authors.lastname: Guan
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Chun-yan
authors.initials: CY
authors.lastname: Liu
authors.forename: Yi-hao
authors.initials: YH
authors.lastname: Wang
authors.forename: Hui-juan
authors.initials: HJ
authors.lastname: Jiang
authors.forename: Kai
authors.initials: K
authors.lastname: Ding
authors.forename: Xi-feng
authors.initials: XF
authors.lastname: Dong
authors.forename: Hong-lei
authors.initials: HL
authors.lastname: Wang
authors.forename: Tian
authors.initials: T
authors.lastname: Zhang
authors.forename: Zong-hong
authors.initials: ZH
authors.lastname: Shao
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000741:Anemia, Aplastic / Q000235:genetics / Q000378:metabolism*; D017252:CD3 Complex / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D000089804:Shelterin Complex; D013601:T-Lymphocytes / Q000378:metabolism*; D016615:Telomere / Q000378:metabolism*; D034501:Telomere-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: C495876:ACD protein, human; D017252:CD3 Complex; D000089804:Shelterin Complex; D034501:Telomere-Binding Proteins