title: Antioxidant response versus selenium accumulation in the liver and kidney of the Siberian sturgeon (Acipenser baeri).
abstract: The aim of this study was to examine the effects of selenium on concentrations of metabolites and enzyme activities acting as antioxidant markers in liver and kidney of Siberian sturgeon Acipenser baeri. Sturgeons were fed selenium cysteine for 30 and 60 d at 1.25, 5, 20mg Sekg(-1). Selenium level in the control feed was 0.32 mg kg(-1). Se concentration was measured in liver, kidney and muscle of every specimen. Sturgeon accumulated Se in tissues with a clear dose-response relationship and the highest Se concentration was recorded in liver. This outcome is lined up with the findings obtained on the antioxidant markers evaluated in both tissues, and in which a dose-response for several biomarkers was recorded in liver. The superoxide dismutase activity in Se-treated fish was generally induced, while catalase activity was lower in liver or unaltered in kidney. The concentrations of glutathione S-transferase, glutathione reductase and total glutathione responded differently for both tissues and were induced in a different way at both endpoints. No changes of glyoxalase I activity were noted for both Se-treated tissues, while for glyoxalase II enzyme in liver a dose-related pattern was found showing a reversible effect (decreased and increased counteractive response) only in the 5 mg kg(-1) group. Moreover, the highest Se concentrations did not cause marked changes in malondialdehyde levels of liver and kidney. The enhancement of glutathione peroxidase activity in Se-treated sturgeon might have prevented the lipid peroxidation in both tissues, providing to the Siberian sturgeon a great defense ability versus the prooxidant effect of selenium.
authors.affiliation: Department of Cellular and Environmental Biology, University of Perugia, 06123 Perugia, Italy.
authors.forename: Nicole
authors.initials: N
authors.lastname: Pacini
authors.forename: Antonia Concetta
authors.initials: AC
authors.lastname: Elia
authors.forename: Maria Cesarina
authors.initials: MC
authors.lastname: Abete
authors.forename: Ambrosius Josef Martin
authors.initials: AJ
authors.lastname: Dörr
authors.forename: Paola
authors.initials: P
authors.lastname: Brizio
authors.forename: Laura
authors.initials: L
authors.lastname: Gasco
authors.forename: Marzia
authors.initials: M
authors.lastname: Righetti
authors.forename: Marino
authors.initials: M
authors.lastname: Prearo
mesh_terms: D000818:Animals; D005399:Fishes / Q000502:physiology*; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012643:Selenium / Q000378:metabolism / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
chemical_list: D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D008315:Malondialdehyde; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione; D012643:Selenium
keywords: Accumulation; Kidney; Liver; Oxidative stress biomarkers; Selenium; Siberian sturgeon