title: 2,4-Diamino-6,7-dimethylpteridine as a fluorescent ligand for binding and sensing an orphan cytosine in RNA duplexes.
abstract: We report on a new fluorescent ligand, 2,4-diamino-6,7-dimethylpteridine, that can strongly and selectively bind to an orphan cytosine opposite an abasic site in RNA duplexes. We describe a significant effect of the substituents attached to the pteridine ring on the binding behavior in comparison with a structurally-similar pteridine derivative.
authors.affiliation: Department of Chemistry, Graduate School of Science, Tohoku University, Sendai 980-8578, Japan. teramae@m.tohoku.ac.jp.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sato
authors.forename: Yu
authors.initials: Y
authors.lastname: Toriyabe
authors.forename: Seiichi
authors.initials: S
authors.lastname: Nishizawa
authors.forename: Norio
authors.initials: N
authors.lastname: Teramae
mesh_terms: D001665:Binding Sites; D003596:Cytosine / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D015394:Molecular Structure; D011621:Pteridines / Q000737:chemistry* / Q000378:metabolism; D012313:RNA / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
chemical_list: D008024:Ligands; D011621:Pteridines; C020280:2,4-diamino-6,7-dimethylpteridine; D012313:RNA; D003596:Cytosine