title: Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping.
abstract: We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.
authors.affiliation: Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas , Araraquara, SP , Brasil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Miyata
authors.forename: Adolfo Carlos Barreto
authors.initials: AC
authors.lastname: Santos
authors.forename: Natália Helena
authors.initials: NH
authors.lastname: Mendes
authors.forename: Eunice Atsuko
authors.initials: EA
authors.lastname: Cunha
authors.forename: Fernando Augusto Fiúza
authors.initials: FA
authors.lastname: de Melo
authors.forename: Clarice Queico Fujimura
authors.initials: CQ
authors.lastname: Leite
keywords: CTAB; DNA extraction; Mycobacterium tuberculosis; thermolysis
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