title: Red blood cells derived from peripheral blood and bone marrow CD34⁺ human haematopoietic stem cells are permissive to Plasmodium parasites infection.
abstract: The production of fully functional human red cells in vitro from haematopoietic stem cells (hHSCs) has been successfully achieved. Recently, the use of hHSCs from cord blood represented a major improvement to develop the continuous culture system for Plasmodium vivax. Here, we demonstrated that CD34⁺ hHSCs from peripheral blood and bone marrow can be expanded and differentiated to reticulocytes using a novel stromal cell. Moreover, these reticulocytes and mature red blood cells express surface markers for entrance of malaria parasites contain adult haemoglobin and are also permissive to invasion by P. vivax and Plasmodium falciparum parasites.
authors.affiliation: Barcelona Centre for International Health Research, Hospital Clinic, Universitat de Barcelona, BarcelonaSpain, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Fernandez-Becerra
authors.forename: Joel
authors.initials: J
authors.lastname: Lelievre
authors.forename: Mireia
authors.initials: M
authors.lastname: Ferrer
authors.forename: Nuria
authors.initials: N
authors.lastname: Anton
authors.forename: Richard
authors.initials: R
authors.lastname: Thomson
authors.forename: Cristina
authors.initials: C
authors.lastname: Peligero
authors.forename: Maria Jesus
authors.initials: MJ
authors.lastname: Almela
authors.forename: Marcus Vg
authors.initials: MV
authors.lastname: Lacerda
authors.forename: Esperanza
authors.initials: E
authors.lastname: Herreros
authors.forename: Hernando A
authors.initials: HA
authors.lastname: Del Portillo
mesh_terms: D018952:Antigens, CD34 / Q000302:isolation & purification*; D002454:Cell Differentiation; D018920:Coculture Techniques / Q000379:methods; D004912:Erythrocytes / Q000469:parasitology*; D006412:Hematopoietic Stem Cells / Q000469:parasitology*; D006801:Humans; D008288:Malaria / Q000097:blood*; D016780:Malaria, Vivax; D010963:Plasmodium falciparum; D012156:Reticulocytes / Q000166:cytology / Q000469:parasitology
chemical_list: D018952:Antigens, CD34
references.citation: Blood. 2011 Nov 10;118(19):5071-9
references.pmid: 21885599
references.citation: Blood. 2011 Sep 29;118(13):e74-81
references.pmid: 21768300
references.citation: Int J Parasitol. 2007 Dec;37(14):1551-7
references.pmid: 17610880
references.citation: Nat Biotechnol. 2005 Jan;23(1):69-74
references.pmid: 15619619
references.citation: Tissue Eng Part C Methods. 2011 Nov;17(11):1131-7
references.pmid: 21736478
references.citation: Blood. 1995 Jun 1;85(11):3127-33
references.pmid: 7756644
references.citation: J Exp Med. 1994 Aug 1;180(2):497-506
references.pmid: 8046329
references.citation: PLoS One. 2011 May 04;6(5):e19307
references.pmid: 21573240
references.citation: PLoS One. 2012;7(7):e40798
references.pmid: 22844411
references.citation: Science. 1976 Aug 20;193(4254):673-5
references.pmid: 781840
references.citation: Mem Inst Oswaldo Cruz. 2011 Aug;106 Suppl 1:79-84
references.pmid: 21881760