title: Detection of Bartonella henselae in defibrinated sheep blood used for culture media supplementation.
abstract: Bartonella henselae was detected in defibrinated sheep blood employed in supplementing a selective bacteria culture medium by nested PCR. We recommended that highly sensitive technical tests be run to ensure a sterile culture medium for Bartonella spp. isolation, since infected blood samples used in preparation could lead to false-positive results.
authors.affiliation: Departamento de Medicina Interna, Faculdade de Ciências Médicas, Universidade Estadual de Campinas , Campinas, SP , Brasil.
authors.forename: Marina Rovani
authors.initials: MR
authors.lastname: Drummond
authors.forename: Luiza H U
authors.initials: LH
authors.lastname: Pitassi
authors.forename: Bruno G
authors.initials: BG
authors.lastname: Lania
authors.forename: Silvio R C
authors.initials: SR
authors.lastname: Dos Santos
authors.forename: Rovilson
authors.initials: R
authors.lastname: Gilioli
authors.forename: Paulo E N F
authors.initials: PE
authors.lastname: Velho
keywords: Bartonella; Culture Media; False Positive Reactions; Polymerase Chain Reaction; Sheep-Blood Contamination
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