title: Identification and structure elucidation of a novel antifungal compound produced by Pseudomonas aeruginosa PGPR2 against Macrophomina phaseolina.
abstract: Pseudomonas aeruginosa PGPR2 was found to protect mungbean plants from charcoal rot disease caused by Macrophomina phaseolina. Secondary metabolites from the culture supernatant of P. aeruginosa PGPR2 were extracted with ethyl acetate and the antifungal compound was purified by preparative HPLC using reverse phase chromatography. The purified compound showed antifungal activity against M. phaseolina and other phytopathogenic fungi (Fusarium sp., Rhizoctonia sp. Alternaria sp., and Aspergillus sp.). The structure of the purified compound was determined using (1)H, (13)C, 2D NMR spectra and liquid chromatography-mass spectrometry (LC-MS). Spectral data suggest that the antifungal compound is 3,4-dihydroxy-N-methyl-4-(4-oxochroman-2-yl)butanamide, with the chemical formula C14H17NO5 and a molecular mass of 279. Though chemically synthesized chromanone derivatives have been shown to have antifungal activity, we report for the first time, the microbial production of a chromanone derivative with antifungal activity. This ability of P. aeruginosa PGPR2 makes it a suitable strain for biocontrol.
authors.affiliation: Department of Genetics, Centre for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai, 625 021, India.
authors.forename: Devaraj
authors.initials: D
authors.lastname: Illakkiam
authors.forename: Paramasivan
authors.initials: P
authors.lastname: Ponraj
authors.forename: Manoharan
authors.initials: M
authors.lastname: Shankar
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Muthusubramanian
authors.forename: Jeyaprakash
authors.initials: J
authors.lastname: Rajendhran
authors.forename: Paramasamy
authors.initials: P
authors.lastname: Gunasekaran
mesh_terms: D000898:Antibiosis; D000935:Antifungal Agents / Q000737:chemistry* / Q000302:isolation & purification; D001203:Ascomycota / Q000187:drug effects / Q000472:pathogenicity; D015394:Molecular Structure; D010935:Plant Diseases / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000737:chemistry*
chemical_list: D000935:Antifungal Agents