title: Expression of miR-15a and miR-16-1 in patients with chronic lymphocytic leukemia.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are small non-coding single-stranded RNA molecules that regulate gene expression at the post-transcriptional level. In the pathogenesis of chronic lymphocytic leukemia (CLL), miR-15a and miR-16-1 play an important role. These miRNAs are located on chromosome 13 in the 13q14.3 region, which is deleted in more than 55% of CLL patients. This aberration affects expression of miRNAs.
  
  
  OBJECTIVE
  The study aimed at performing a molecular genetic analysis of miR-15a and miR-16-1 expression in a group of 39 patients diagnosed with CLL and determining the association between the expression of the two miRNAs and types of deletions in the 13q14 region.
  
  
  METHODS
  We used fluorescence in situ hybridiziation (FISH) for determination of mono- or biallelic deletion 13q and quantitative polymerase chain reaction (Q-RT-PCR) to revealed expression miR-15a and miR-16-1 in 39 patients suffering from CLL.
  
  
  RESULTS
  The analysis comprised 19 patients with monoallelic 13q14 deletion, 3 patients with biallelic deletion, 9 patients with both monoallelic and biallelic deletions, and 8 patients without 13q14 deletion serving as controls. The results showed different levels of miRNA expression in individual patients. Significantly higher normalized levels of miR-15a expression were found in the control group and patients with monoallelic 13q14 expression compared with patients with biallelic deletion. There was a significantly decreased expression of both miRNAs in patients with biallelic deletion of the 13q14 region but only when deletions were present in 77% or more of cells, as detected by fluorescent in situ hybridization (FISH).
authors.affiliation: Department of Hemato-Oncology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Humplikova
authors.forename: Sona
authors.initials: S
authors.lastname: Kollinerova
authors.forename: Tomas
authors.initials: T
authors.lastname: Papajik
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Pikalova
authors.forename: Milena
authors.initials: M
authors.lastname: Holzerova
authors.forename: Vit
authors.initials: V
authors.lastname: Prochazka
authors.forename: Martina
authors.initials: M
authors.lastname: Divoka
authors.forename: Martin
authors.initials: M
authors.lastname: Modriansky
authors.forename: Karel
authors.initials: K
authors.lastname: Indrak
authors.forename: Marie
authors.initials: M
authors.lastname: Jarosova
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D008875:Middle Aged
chemical_list: C505361:MIRN15 microRNA, human; C505362:MIRN16 microRNA, human; D035683:MicroRNAs