title: [Evaluation of the reliability of thiophen-2-carboxylic acid hydrazine result for the identification of Mycobacterium bovis].
abstract: OBJECTIVE
  To evaluate the reliability of thiophen-2-carboxylic acid hydrazine (TCH) test for identification of Mycobacterium bovis (M. bovis).
  
  
  METHODS
  A total of 4069 clinically isolated strains were identified by P-Nitrobenzoic acid medium (500 mg/L) and TCH medium (5 mg/L). Mycobacterium tuberculosis (M. tuberculosis) complex strains susceptible to 5 mg/L TCH were further tested for susceptibility to 2 mg/L TCH. Spacer oligonucleotide typing (spoligotyping) and multi-loci PCR were also performed to identify TCH susceptible strains.
  
  
  RESULTS
  Among the 4069 isolated strains there were 3929 strains belonging to M. tuberculosis complex (MTBC) of which 245 were susceptible to 5 mg/L TCH. Of these 245 strains, 20 were also susceptible to 2 mg/L TCH, while only 1 strain was identified to be M. bovis by both spoligotyping and multi-loci PCR.
  
  
  CONCLUSIONS
  TCH susceptibility test (either 5 mg/L or 2 mg/L) is not a reliable method for identification of M. bovis.
authors.affiliation: National Tuberculosis Clinical Laboratory, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
authors.forename: Guang-lu
authors.initials: GL
authors.lastname: Jiang
authors.forename: Qian
authors.initials: Q
authors.lastname: Liang
authors.forename: Yu-hong
authors.initials: YH
authors.lastname: Fu
authors.forename: Ling-ling
authors.initials: LL
authors.lastname: Dong
authors.forename: Yan-lin
authors.initials: YL
authors.lastname: Zhao
authors.forename: Hai-rong
authors.initials: HR
authors.lastname: Huang
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002417:Cattle; D004269:DNA, Bacterial / Q000235:genetics; D008826:Microbial Sensitivity Tests; D009163:Mycobacterium bovis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D013876:Thiophenes / Q000008:administration & dosage / Q000494:pharmacology*; D014376:Tuberculosis / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D013876:Thiophenes; C016144:thiophene-2-carboxylic acid hydrazide