title: Improving the success rate of protein crystallization by random microseed matrix screening.
abstract: Random microseed matrix screening (rMMS) is a protein crystallization technique in which seed crystals are added to random screens. By increasing the likelihood that crystals will grow in the metastable zone of a protein's phase diagram, extra crystallization leads are often obtained, the quality of crystals produced may be increased, and a good supply of crystals for data collection and soaking experiments is provided. Here we describe a general method for rMMS that may be applied to either sitting drop or hanging drop vapor diffusion experiments, established either by hand or using liquid handling robotics, in 96-well or 24-well tray format.
authors.affiliation: School of Biochemistry, University of Bristol.
authors.forename: Marisa
authors.initials: M
authors.lastname: Till
authors.forename: Alice
authors.initials: A
authors.lastname: Robson
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Byrne
authors.forename: Asha V
authors.initials: AV
authors.lastname: Nair
authors.forename: Stefan A
authors.initials: SA
authors.lastname: Kolek
authors.forename: Patrick D
authors.initials: PD
authors.lastname: Shaw Stewart
authors.forename: Paul R
authors.initials: PR
authors.lastname: Race
mesh_terms: D003460:Crystallization / Q000295:instrumentation / Q000379:methods*; D004058:Diffusion; D005740:Gases / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012371:Robotics / Q000379:methods
chemical_list: D005740:Gases; D011506:Proteins
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