title: Lateral flow biosensors for the detection of nucleic acid.
abstract: The detection of nucleic acid is of central importance for the diagnosis of genetic diseases, infectious agents, and biowarfare agents. Traditional strategies and technologies for nucleic acid detection are time-consuming and labor-intensive. Recently, isothermal strand-displacement reaction-based lateral flow biosensors have attracted a great deal of research interest because they are sensitive, simple, fast, and easy to use. Here, we describe a lateral flow biosensor based on isothermal strand-displacement polymerase reaction and gold nanoparticles for the visual detection of nucleic acid.
authors.affiliation: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
authors.forename: Lingwen
authors.initials: L
authors.lastname: Zeng
authors.forename: Puchang
authors.initials: P
authors.lastname: Lie
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Fang
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Xiao
mesh_terms: D000818:Animals; D001483:Base Sequence; D015374:Biosensing Techniques; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics; D006046:Gold / Q000737:chemistry; D016174:Hepacivirus / Q000235:genetics; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D009169:Mycobacterium tuberculosis / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D004279:DNA, Viral; D006046:Gold