title: Synthesis of molecularly imprinted polymer sorbents and application for the determination of aminoglycosides antibiotics in honey.
abstract: Aminoglycoside antibiotic (AA)-selective molecularly imprinted polymer (MIP) sorbent was synthesized by polymerization of methacrylic acid and ethylene glycol dimethacrylate in the presence of streptomycin as template molecule. The MIP sorbent was in detail characterized and its performance was evaluated by selecting four model AAs including streptomycin, gentamicin, spectinomycin and dihydrostreptomycin. Relative to non-imprinted polymer (NIP), the MIPs exhibited much higher recognizable capacity and specificity to the AAs and negligible adsorption for non-AAs as well. In combination of hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS), the MIP sorbent-based solid phase extraction (SPE) was able to effectively determine the residue of four model AAs in honey samples with recovery ranging from 90% to 110%. The limit of detection (and the limit of quantitation) of streptomycin, gentamicin, spectinomycin and dihydrostreptomycin was 4.5 (15.0), 2.4 (8.0), 6.0 (20.0) and 1.8 (6.0) μg/kg, respectively. Relative standard deviations of intraday and inter-day assay under three spiked levels were 4.4-12.0% and 6.8-14.6%, respectively.
authors.affiliation: School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Shunli
authors.initials: S
authors.lastname: Ji
authors.forename: Feifang
authors.initials: F
authors.lastname: Zhang
authors.forename: Xi
authors.initials: X
authors.lastname: Luo
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Yang
authors.forename: Gaowa
authors.initials: G
authors.lastname: Jin
authors.forename: Jingyu
authors.initials: J
authors.lastname: Yan
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D000327:Adsorption; D000617:Aminoglycosides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D002853:Chromatography, Liquid / Q000379:methods; D006722:Honey / Q000032:analysis*; D057230:Limit of Detection; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D011108:Polymers
keywords: Aminoglycoside antibiotics; HILIC–MS/MS; Honey samples; Molecularly imprinted polymer; Solid phase extraction