title: Electrochemical biosensors for biocontaminant detection consisting of carbon nanotubes, platinum nanoparticles, dendrimers, and enzymes.
abstract: The presented approach provides the advanced development of effective, rapid, and versatile electrochemical sensors for a small amount of analytes on potential, cheap, and disposable printed chips. The electrocatalytic activity of this biosensor revealed the feasible detection of hydrogen peroxide at low potential (~0.09 V) and the detection of a biocontaminant inhibitor (organophosphorus pesticide) in a wide range of concentrations. This efficiency comes from the chemical immobilization of catalysts (Pt nanoparticles) and electron transfer-enlarging materials (carbon nanotubes) on an electrode. Especially, dendrimers raise the stable conjugation of enzymes (acetylcholinesterase/choline oxidase/peroxidase) as well as nanoparticles and carbon nanotubes on an electrode.
authors.affiliation: Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 10607, Taiwan, ROC.
authors.forename: Ampornphan
authors.initials: A
authors.lastname: Siriviriyanun
authors.forename: Toyoko
authors.initials: T
authors.lastname: Imae
authors.forename: Naoki
authors.initials: N
authors.lastname: Nagatani
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation*; D050091:Dendrimers / Q000737:chemistry; D055664:Electrochemical Techniques / Q000191:economics / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000378:metabolism; D004867:Equipment Design; D006861:Hydrogen Peroxide / Q000032:analysis*; D053758:Nanoparticles / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D010984:Platinum / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D050091:Dendrimers; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D009943:Organophosphorus Compounds; C104700:PAMAM Starburst; D010575:Pesticides; D010984:Platinum; D006861:Hydrogen Peroxide; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D000110:Acetylcholinesterase
keywords: Electrochemical biosensor; Enzyme reaction; Inhibition reaction; Multiwalled carbon nanotube; Platinum nanoparticle; Poly(amido amine) dendrimer