title: [Opportunistic bacteria and microbial flora in children with leukemia and neutropenic enterocolitis].
abstract: OBJECTIVE
  to determine the prevalence of opportunistic microorganisms and microbial flora in neutropenic enterocolitis in oncohematological pediatric patients.
  
  
  METHODS
  a prospective and observational study was done. Patients with diagnosis of acute leukemia and neutropenia were included. Stool cultures were taken to identify microorganisms and microbial flora. A χ(2) test with Yates corrections and Fisher exact test were used in the statistical analysis.
  
  
  RESULTS
  21 patients were included (12 male, 57.1 %). The stool cultures showed that 68 % of microorganisms were Gram-negative. The presence of microorganisms Gram-positive was 20 %, 6 % for Candida sp.; 3 % for Cryptosporidium sp.; and in 3 % were acid fast bacilli. Staphylococcus epidermidis, Enterobacter sp., and Escherichia coli were presented in pure culture. No association was found between Gram-positive and Gram-negative microorganisms with age, white cell count or pure or mixed cultures.
  
  
  CONCLUSIONS
  although Gram-negative microorganisms were the most frequent, Gram-positive and other microorganisms that are not detected habitually in feces culture were isolated.
authors.affiliation: Comité de Investigación y Bioética, Hospital de Infectología, Distrito Federal, Mexico. gelorriaga@webtelmex.net.mx.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: García-Elorriaga
authors.forename: Juan C
authors.initials: JC
authors.lastname: Corona-de Los Santos
authors.forename: Socorro
authors.initials: S
authors.lastname: Méndez-Tovar
authors.forename: Guillermo
authors.initials: G
authors.lastname: del Rey-Pineda
authors.forename: Ruy X
authors.initials: RX
authors.lastname: Pérez-Casillas
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D044504:Enterocolitis, Neutropenic / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D015470:Leukemia, Myeloid, Acute / Q000382:microbiology*; D008297:Male; D009894:Opportunistic Infections / Q000382:microbiology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000382:microbiology*; D011446:Prospective Studies
keywords: enterocolitis neutropénica; hematology; hematología; metagenoma; neutropenic enterocolitis