title: Intraspecific 16S rRNA gene diversity among clinical isolates of Neisseria species.
abstract: In the present work, nearly the entire 16S rRNA gene sequences of 46 clinical samples of Neisseria spp. were determined, and the aligned sequences were analyzed to investigate the diversity of 16S rRNA genes in each commensal Neisseria species. Two 16S rRNA types were identified in two Neisseria sicca strains, three 16S rRNA types in five Neisseria macacae strains, fourteen 16S rRNA types in twenty Neisseria flavescens isolates, and fourteen 16S rRNA types in nineteen Neisseria mucosa isolates. The number of nucleotides that were different between 16S rRNA sequences within specie ranged from 1 to 15. We found high intraspecific sequence variation in 16S rRNA genes of Neisseria spp. strains.
authors.affiliation: Service des Laboratoires, Centre National de Greffe de Moelle Osseuse de Tunis, Faculté de Médecine de Tunis, Tunis, Tunisia.
authors.forename: Arij
authors.initials: A
authors.lastname: Mechergui
authors.forename: Wafa
authors.initials: W
authors.lastname: Achour
authors.forename: Assia Ben
authors.initials: AB
authors.lastname: Hassen
mesh_terms: D000595:Amino Acid Sequence; D015373:Bacterial Typing Techniques; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009343:Neisseria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA; Neisseria spp.; genetic diversity