title: Triterpenes from Euphorbia spinidens with immunomodulatory activity.
abstract: Dried aceton-chloroform extract of aerial parts of Euphorbia spinidens Bornm. ex Prokh. endemic to Iran, yielded two new triterpenoids, lup-20(29)-ene-33, 28 diol commonly known as betulin and (3β,23E)-Cycloarta-23-ene-3,25-diol. The structures of the isolated compounds were elucidated by 13C- and 1H-NMR as well as 2D-NMR, IR and by the aid of mass fragmentation pattern. In phagocyte chemiluminescence assay, different concentrations of compounds were incubated with the human whole blood in triplicate and the chemiluminescence activity of phagocytic cells were measured by using serum opsonized zymosan and luminol. For lymphocyte proliferation assay, peripheral human blood lymphocytes were incubated with different concentrations of the test compound in supplemented Roswell Park Memorial Institute (RPMI) 1640 medium along with 5.0 μg/ml phytohemagglutinin (PHA) at 37°C in CO2 environment for 72 h and proliferation level was determined by Beta-scintillation counter. In phagocyte chemiluminescence assay, betulin showed moderate inhibitory effect on the oxidative burst in the neutrophils, while addition of betulin triterpene was able to stimulate the proliferation of the phytohemagglutinin (PHA) treated human peripheral blood lymphocytes (hPBLs).
authors.affiliation: Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
authors.forename: M
authors.initials: M
authors.lastname: Ghannadian
authors.forename: A
authors.initials: A
authors.lastname: Akhavan
authors.forename: O M
authors.initials: OM
authors.lastname: Abdalla
authors.forename: A M
authors.initials: AM
authors.lastname: Ayatollahi
authors.forename: M
authors.initials: M
authors.lastname: Mohammadi-Kamalabadi
authors.forename: H
authors.initials: H
authors.lastname: Ghazanfari
keywords: Euphorboa spinidens; Immunomodulation; Iran; cycloartane; hPBLs; lupane type triterpenes
references.citation: Curr Med Chem. 2005;12(6):657-66
references.pmid: 15790304
references.citation: Toxicology. 2011 Feb 27;280(3):152-63
references.pmid: 21172400
references.citation: Clin Chim Acta. 2002 Nov;325(1-2):91-6
references.pmid: 12367771
references.citation: IDrugs. 2004 Apr;7(4):359-73
references.pmid: 15057642
references.citation: Bioorg Med Chem. 2006 Feb 15;14(4):938-43
references.pmid: 16202612
references.citation: Iran J Pharm Res. 2011 Winter;10(1):105-12
references.pmid: 24363688
references.citation: Phytomedicine. 2010 Apr;17(5):379-82
references.pmid: 19748254
references.citation: Exp Clin Immunogenet. 1998;15(2):61-8
references.pmid: 9691200
references.citation: Phytochemistry. 2006 Sep;67(18):1977-84
references.pmid: 16889806