title: Comprehensive analysis of genic male sterility-related genes in Brassica rapa using a newly developed Br300K oligomeric chip.
abstract: To identify genes associated with genic male sterility (GMS) that could be useful for hybrid breeding in Chinese cabbage (Brassicarapa ssp. pekinensis), floral bud transcriptome analysis was carried out using a B. rapa microarray with 300,000 probes (Br300K). Among 47,548 clones deposited on a Br300K microarray with seven probes of 60 nt length within the 3' 150 bp region, a total of 10,622 genes were differentially expressed between fertile and sterile floral buds; 4,774 and 5,848 genes were up-regulated over 2-fold in fertile and sterile buds, respectively. However, the expression of 1,413 and 199 genes showed fertile and sterile bud-specific features, respectively. Genes expressed specifically in fertile buds, possibly GMS-related genes, included homologs of several Arabidopsis male sterility-related genes, genes associated with the cell wall and synthesis of its surface proteins, pollen wall and coat components, signaling components, and nutrient supplies. However, most early genes for pollen development, genes for primexine and callose formation, and genes for pollen maturation and anther dehiscence showed no difference in expression between fertile and sterile buds. Some of the known genes associated with Arabidopsis pollen development showed similar expression patterns to those seen in this study, while others did not. BrbHLH89 and BrMYP99 are putative GMS genes. Additionally, 17 novel genes identified only in B. rapa were specifically and highly expressed only in fertile buds, implying the possible involvement in male fertility. All data suggest that Chinese cabbage GMS might be controlled by genes acting in post-meiotic tapetal development that are different from those known to be associated with Arabidopsis male sterility.
authors.affiliation: Department of Biological Sciences, Chungnam National University, Daejeon, Korea.
authors.forename: Xiangshu
authors.initials: X
authors.lastname: Dong
authors.forename: Hui
authors.initials: H
authors.lastname: Feng
authors.forename: Ming
authors.initials: M
authors.lastname: Xu
authors.forename: Jeongyeo
authors.initials: J
authors.lastname: Lee
authors.forename: Yeon Ki
authors.initials: YK
authors.lastname: Kim
authors.forename: Yong Pyo
authors.initials: YP
authors.lastname: Lim
authors.forename: Zhongyun
authors.initials: Z
authors.lastname: Piao
authors.forename: Young Doo
authors.initials: YD
authors.lastname: Park
authors.forename: Hong
authors.initials: H
authors.lastname: Ma
authors.forename: Yoonkang
authors.initials: Y
authors.lastname: Hur
mesh_terms: D029741:Brassica rapa / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D016000:Cluster Analysis; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005838:Genotype; D020411:Oligonucleotide Array Sequence Analysis; D051479:Plant Infertility / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011058:Pollen / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D003577:Cytochrome P-450 Enzyme System
references.citation: Plant Cell. 2006 Nov;18(11):2999-3014
references.pmid: 17138695
references.citation: Phytochemistry. 2009 Dec;70(17-18):1918-29
references.pmid: 19818976
references.citation: Plant J. 2007 Jun;50(5):751-66
references.pmid: 17419837
references.citation: Plant Physiol. 2010 Aug;153(4):1445-52
references.pmid: 20508141
references.citation: Genes Dev. 2002 Aug 1;16(15):2021-31
references.pmid: 12154130
references.citation: Plant Cell. 2001 Jul;13(7):1625-38
references.pmid: 11449055
references.citation: Plant Cell. 2005 Mar;17(3):705-21
references.pmid: 15722475
references.citation: Plant Cell Physiol. 2007 Jun;48(6):812-21
references.pmid: 17468126
references.citation: Genome Biol. 2004;5(11):R85
references.pmid: 15535861
references.citation: J Exp Bot. 2009;60(5):1479-92
references.pmid: 19321648
references.citation: Plant Cell. 2004;16 Suppl:S142-53
references.pmid: 15037731
references.citation: Plant Physiol. 2009 Feb;149(2):694-707
references.pmid: 19091874
references.citation: Proc Natl Acad Sci U S A. 2008 Feb 12;105(6):2220-5
references.pmid: 18250314
references.citation: Plant Cell Physiol. 2011 Sep;52(9):1459-66
references.pmid: 21743085
references.citation: Plant Sci. 2013 Feb;199-200:7-17
references.pmid: 23265314
references.citation: Genome. 1998 Feb;41(1):62-9
references.pmid: 9549059
references.citation: Plant Physiol. 2003 Jun;132(2):640-52
references.pmid: 12805594
references.citation: Plant J. 2008 Jul;55(2):266-77
references.pmid: 18397379
references.citation: Science. 2009 Sep 25;325(5948):1688-92
references.pmid: 19779199
references.citation: Plant Cell. 2010 Jan;22(1):91-107
references.pmid: 20118226
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:627-654
references.pmid: 15012303
references.citation: Plant Mol Biol. 2008 Jan;66(1-2):177-92
references.pmid: 18040866
references.citation: Plant Cell. 2004;16 Suppl:S46-60
references.pmid: 15131249
references.citation: Plant Physiol. 2009 Nov;151(3):1401-11
references.pmid: 19726570
references.citation: Plant Cell. 2008 Oct;20(10):2631-42
references.pmid: 18849494
references.citation: Planta. 2006 Feb;223(3):613-7
references.pmid: 16283376
references.citation: Plant Cell. 1999 Mar;11(3):431-44
references.pmid: 10072402
references.citation: J Exp Bot. 2003 Jan;54(382):525-31
references.pmid: 12508063
references.citation: Curr Opin Plant Biol. 2011 Feb;14(1):66-73
references.pmid: 21145279
references.citation: Plant J. 1993 Apr;3(4):495-507
references.pmid: 8220457
references.citation: Plant J. 1998 Aug;15(3):345-56
references.pmid: 9750346
references.citation: Plant Cell Rep. 2009 Sep;28(9):1363-72
references.pmid: 19562345
references.citation: Plant Physiol. 2005 Jun;138(2):744-56
references.pmid: 15908605
references.citation: Plant Cell Physiol. 1995 Dec;36(8):1599-606
references.pmid: 8589934
references.citation: Nucleic Acids Res. 2003 Feb 15;31(4):e15
references.pmid: 12582260
references.citation: Development. 2000 Oct;127(19):4227-38
references.pmid: 10976054
references.citation: Sex Plant Reprod. 2010 Sep;23(3):199-205
references.pmid: 20162305
references.citation: Plant J. 2008 Nov;56(3):351-63
references.pmid: 18644001
references.citation: Plant Physiol. 2007 Nov;145(3):747-62
references.pmid: 17905860
references.citation: Genome Biol. 2002 Aug 30;3(9):research0048
references.pmid: 12225587
references.citation: Plant Cell. 2011 May;23(5):1932-44
references.pmid: 21540435
references.citation: Plant J. 1999 Feb;17(3):321-7
references.pmid: 10097390
references.citation: Plant Cell Physiol. 2002 Nov;43(11):1285-92
references.pmid: 12461128
references.citation: Protoplasma. 2006 Aug;228(1-3):31-9
references.pmid: 16937052
references.citation: Proteomics. 2006 Jan;6(1):301-11
references.pmid: 16287169
references.citation: Plant J. 2003 Jan;33(2):413-23
references.pmid: 12535353
references.citation: Plant Mol Biol. 1998 Jul;37(4):607-19
references.pmid: 9687065
references.citation: Plant Cell. 2007 Nov;19(11):3530-48
references.pmid: 18032629
references.citation: Trends Plant Sci. 2005 Feb;10(2):79-87
references.pmid: 15708345
references.citation: Plant Cell. 2000 Jan;12(1):151-64
references.pmid: 10634914
references.citation: Plant Cell. 2005 Oct;17(10):2705-22
references.pmid: 16141453
references.citation: Plant J. 2006 Jun;46(6):984-1008
references.pmid: 16805732
references.citation: Annu Rev Plant Biol. 2005;56:393-434
references.pmid: 15862102
references.citation: Annu Rev Plant Biol. 2011;62:437-60
references.pmid: 21275644
references.citation: Development. 2006 Aug;133(16):3085-95
references.pmid: 16831835
references.citation: Nat Genet. 2011 Aug 28;43(10):1035-9
references.pmid: 21873998
references.citation: Science. 2001 Jun 29;292(5526):2482-5
references.pmid: 11431566
references.citation: Plant Cell. 2005 Dec;17(12):3337-49
references.pmid: 16284305
references.citation: Curr Biol. 2002 Oct 15;12(20):1718-27
references.pmid: 12401166
references.citation: Plant Cell. 1993 Oct;5(10):1217-29
references.pmid: 8281038
references.citation: Genes Dev. 1999 Aug 15;13(16):2108-17
references.pmid: 10465788
references.citation: Plant Cell. 2004 May;16(5):1314-26
references.pmid: 15100403
references.citation: Ann Bot. 2011 Sep;108(4):627-36
references.pmid: 21307038
references.citation: Plant Cell Rep. 2008 Nov;27(11):1741-54
references.pmid: 18758783
references.citation: Sex Plant Reprod. 2009 Sep;22(3):197-204
references.pmid: 20033440
references.citation: Plant Physiol. 2011 Mar;155(3):1403-15
references.pmid: 21257792
references.citation: Plant Cell. 2005 Dec;17(12):3350-61
references.pmid: 16284306
references.citation: Plant Mol Biol. 2010 Jan;72(1-2):111-23
references.pmid: 19862484
references.citation: Curr Biol. 2010 Nov 23;20(22):R988-97
references.pmid: 21093795
references.citation: BMC Plant Biol. 2009 Jun 03;9:68
references.pmid: 19493335
references.citation: Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5116-21
references.pmid: 11309499
references.citation: Nat Genet. 2007 Sep;39(9):1156-61
references.pmid: 17721535
references.citation: Plant Cell. 2007 Nov;19(11):3549-62
references.pmid: 18032630
references.citation: Int J Dev Biol. 2005;49(5-6):595-614
references.pmid: 16096968
references.citation: Plant Biotechnol J. 2004 Jan;2(1):45-57
references.pmid: 17166142
references.citation: Plant Physiol. 2009 Aug;150(4):1822-30
references.pmid: 19493971
references.citation: Plant J. 2007 Oct;52(1):14-29
references.pmid: 17666023
references.citation: Proc Natl Acad Sci U S A. 2010 Mar 30;107(13):6100-5
references.pmid: 20231470
references.citation: Plant Physiol. 2003 Oct;133(2):713-25
references.pmid: 14500793
references.citation: Plant J. 2007 Nov;52(3):528-38
references.pmid: 17727613
references.citation: J Exp Bot. 2010 Jun;61(10):2693-706
references.pmid: 20427415
references.citation: Plant Cell. 2003 Jan;15(1):207-22
references.pmid: 12509532