title: Clinical peptidomic analysis by a one-step direct transfer technology: its potential utility for monitoring of pathophysiological status in female reproductive system disorders.
abstract: To date, numerous studies have searched for candidate molecules or clinical examination methods as potential biomarkers for monitoring intractable diseases, such as carcinomas. Evidence accumulated over the past decade shows that many proteolytic peptides appear in human humoral fluids, including peripheral blood, in association with an individual's health condition. Although an analysis of the whole peptide (the 'peptidome') using mass spectrometry is thought to be one of the most powerful and promising experimental approaches, it has failed to identify biomarkers in the clinical blood samples, presumably due to the methodological limitations. In general, commonly used techniques for proteomic analysis of blood require the removal of large amounts of serum/plasma proteins prior to mass spectrometry analysis, and this step seems to have resulted in the overlooking of important biomarkers during the analytical process. Here, we provide a brief overview of a new quantitative peptidomic analysis by a one-step direct transfer technology without depletion of major blood proteins. Using this technology, we herein report experimental data on serum peptidomic analysis for patients with pregnancy-induced hypertension as a clinical model. In addition, we refer to the potential utility of this approach for the monitoring of pathophysiological status in female reproductive system disorders in general.
authors.affiliation: Institute for Environmental and Gender-specific Medicine, Juntendo University Graduate School of Medicine, Chiba, Japan; Department of Obstetrics and Gynecology, Juntendo University Graduate School of Medicine, Tokyo, Japan.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Araki
authors.forename: Daisuke
authors.initials: D
authors.lastname: Nonaka
authors.forename: Kensuke
authors.initials: K
authors.lastname: Hamamura
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Yanagida
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ishikawa
authors.forename: Michio
authors.initials: M
authors.lastname: Banzai
authors.forename: Mayuko
authors.initials: M
authors.lastname: Maruyama
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Endo
authors.forename: Atsushi
authors.initials: A
authors.lastname: Tajima
authors.forename: Lyang-Ja
authors.initials: LJ
authors.lastname: Lee
authors.forename: Michio
authors.initials: M
authors.lastname: Nojima
authors.forename: Kenji
authors.initials: K
authors.lastname: Takamori
authors.forename: Koyo
authors.initials: K
authors.lastname: Yoshida
authors.forename: Satoru
authors.initials: S
authors.lastname: Takeda
authors.forename: Kenji
authors.initials: K
authors.lastname: Tanaka
mesh_terms: D005260:Female; D005831:Genital Diseases, Female / Q000378:metabolism* / Q000503:physiopathology; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000378:metabolism; D040901:Proteomics / Q000639:trends*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018581:Technology Transfer
keywords: BLOTCHIP analysis; clinical biomarker; peptidome; pregnancy-induced hypertension