title: MicroRNA expression profiles in Kaposi's sarcoma.
abstract: Kaposi's sarcoma (KS) is a mesenchymal tumor, caused by Human herpesvirus 8 (HHV8) with molecular and cytogenetic changes poorly understood. To gain further insight on the underlying molecular changes in KS, we performed microRNA (miRNA) microarray analysis of 17 Kaposi's sarcoma specimens. Three normal skin specimens were used as controls. The most significant differentially expressed miRNA were confirmed by quantitative reverse transcriptase polymerase chain reaction (RT-PCR). We detected in KS versus normal skin 185 differentially expressed miRNAs, 76 were upregulated and 109 were downregulated. The most significantly downregulated miRNAs were miR-99a, miR-200 family, miR-199b-5p, miR-100 and miR-335, whereas kshv-miR-K12-4-3p, kshv-miR-K12-1, kshv-miR-K12-2, kshv-miR-K12-4-5p and kshv-miR-K12-8 were significantly upregulated. High expression levels of kshv-miR-K12-1 (p = 0.004) and kshv-miR-K12-4-3p (p = 0.001) was confirmed by RT-PCR. The predicted target genes for differentially expressed miRNAs included genes which are involved in a variety of cellular processes such as angiogenesis (i.e. THBS1) and apoptosis (i.e. CASP3, MCL1), suggesting a role for these miRNAs in Kaposi's sarcoma pathogenesis.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 050095, Bucharest, Romania.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Catrina Ene
authors.forename: Ioana
authors.initials: I
authors.lastname: Borze
authors.forename: Mohamed
authors.initials: M
authors.lastname: Guled
authors.forename: Mariana
authors.initials: M
authors.lastname: Costache
authors.forename: Gayle
authors.initials: G
authors.lastname: Leen
authors.forename: Maria
authors.initials: M
authors.lastname: Sajin
authors.forename: Elena
authors.initials: E
authors.lastname: Ionica
authors.forename: Aura
authors.initials: A
authors.lastname: Chitu
authors.forename: Sakari
authors.initials: S
authors.lastname: Knuutila
mesh_terms: D017209:Apoptosis / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D015870:Gene Expression; D019288:Herpesvirus 8, Human / Q000302:isolation & purification; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000235:genetics; D012514:Sarcoma, Kaposi / Q000098:blood supply / Q000235:genetics* / Q000473:pathology / Q000821:virology; D012867:Skin / Q000473:pathology; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs
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