title: Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish.
abstract: Chikungunya Virus (CHIKV), a re-emerging arbovirus that may cause severe disease, constitutes an important public health problem. Herein we describe a novel CHIKV infection model in zebrafish, where viral spread was live-imaged in the whole body up to cellular resolution. Infected cells emerged in various organs in one principal wave with a median appearance time of ∼14 hours post infection. Timing of infected cell death was organ dependent, leading to a shift of CHIKV localization towards the brain. As in mammals, CHIKV infection triggered a strong type-I interferon (IFN) response, critical for survival. IFN was mainly expressed by neutrophils and hepatocytes. Cell type specific ablation experiments further demonstrated that neutrophils play a crucial, unexpected role in CHIKV containment. Altogether, our results show that the zebrafish represents a novel valuable model to dynamically visualize replication, pathogenesis and host responses to a human virus.
authors.affiliation: Institut Pasteur, Macrophages et Développement de l'Immunité, Department of Developmental and Stem Cells Biology, Paris, France ; CNRS URA2578, Paris, France ; Université Pierre et Marie Curie, Paris, France.
authors.forename: Nuno
authors.initials: N
authors.lastname: Palha
authors.forename: Florence
authors.initials: F
authors.lastname: Guivel-Benhassine
authors.forename: Valérie
authors.initials: V
authors.lastname: Briolat
authors.forename: Georges
authors.initials: G
authors.lastname: Lutfalla
authors.forename: Marion
authors.initials: M
authors.lastname: Sourisseau
authors.forename: Felix
authors.initials: F
authors.lastname: Ellett
authors.forename: Chieh-Huei
authors.initials: CH
authors.lastname: Wang
authors.forename: Graham J
authors.initials: GJ
authors.lastname: Lieschke
authors.forename: Philippe
authors.initials: P
authors.lastname: Herbomel
authors.forename: Olivier
authors.initials: O
authors.lastname: Schwartz
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Levraud
mesh_terms: D018354:Alphavirus Infections / Q000378:metabolism* / Q000473:pathology*; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology / Q000821:virology; D002460:Cell Line; D065632:Chikungunya Fever; D002646:Chikungunya virus / Q000378:metabolism*; D006224:Cricetinae; D004195:Disease Models, Animal; D022781:Hepatocytes / Q000378:metabolism / Q000473:pathology / Q000821:virology; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis*; D009504:Neutrophils / Q000378:metabolism / Q000473:pathology / Q000821:virology; D009928:Organ Specificity; D029961:Zebrafish Proteins / Q000096:biosynthesis*
chemical_list: D007370:Interferon Type I; D029961:Zebrafish Proteins
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