title: The spinning task: a new protocol to easily assess motor coordination and resistance in zebrafish.
abstract: The increasing use of adult zebrafish in behavioral studies has created the need for new and improved protocols. Our investigation sought to evaluate the swimming behavior of zebrafish against a water current using the newly developed Spinning Task. Zebrafish were individually placed in a beaker containing a spinning magnetic stirrer and their latency to be swept into the whirlpool was recorded. We characterized that larger fish (>4 cm) and lower rpm decreased the swimming time in the Spinning Task. There was also a dose-related reduction in swimming after acute treatment with haloperidol, valproic acid, clonazepam, and ethanol, which alter coordination. Importantly, at doses that reduced swimming time in the Spinning Task, these drugs influenced absolute turn angle (ethanol increased and the other drugs decreased), but had no effect of distance travelled in a regular water tank. These results suggest that the Spinning Task is a useful protocol to add information to the assessment of zebrafish motor behavior.
authors.affiliation: 1 Laboratório de Neuroquímica e Psicofarmacologia, Departamento de Biologia Celular e Molecular, Programa de Pós-Graduação em Biologia Celular e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul , Porto Alegre, Brazil .
authors.forename: Ana R
authors.initials: AR
authors.lastname: Blazina
authors.forename: Mônica R
authors.initials: MR
authors.lastname: Vianna
authors.forename: Diogo R
authors.initials: DR
authors.lastname: Lara
mesh_terms: D032761:Animal Experimentation; D000818:Animals; D049628:Body Size; D002998:Clonazepam; D000431:Ethanol; D005260:Female; D006220:Haloperidol; D008124:Locomotion; D008297:Male; D011597:Psychomotor Performance; D012737:Sex Factors; D013550:Swimming / Q000502:physiology; D014635:Valproic Acid; D015027:Zebrafish / Q000502:physiology*
chemical_list: D000431:Ethanol; D002998:Clonazepam; D014635:Valproic Acid; D006220:Haloperidol