title: Isolation and identification of Profenofos degrading bacteria.
abstract: An enrichment culture technique was used to isolate bacterial strains responsible for the biodegradation of profenofos in a soil from Hubei province of central China. Two pure bacterial cultures, named W and Y, were isolated and subsequently characterized by sequencing of 16S rRNA genes and biochemical tests. Isolate W showed 96% similarity to the 16S rRNA gene of a Pseudomonas putida unlike Y which showed 99% similarity to the 16S rRNA gene of Burkholderia gladioli. Both strains grew well at pH 5.5-7.2 with a broad temperature profile ranging from 28° to 36 °C. Bioremediation of profenofos-contaminated soil was examined using soil treated with 200 ug g(-1); profenofos resulted in a higher degradation rate than control soils without inoculation. In a mineral salt medium (FTW) reduction in profenofos concentration was 90% within 96 hours of incubation. A literature survey revealed that no data is available regarding the role of Burkholderia gladioli on pesticide biodegradation as well as on profenofos.
authors.affiliation: Key Laboratory of Biogeology and Environmental Geology, Ministry of Education (BGEG), School of Environmental Studies, China University of Geosciences , 388 Lumo Road, Wuhan, Hubei 430074 , China.
authors.forename: Saadatullah
authors.initials: S
authors.lastname: Malghani
authors.forename: Nivedita
authors.initials: N
authors.lastname: Chatterjee
authors.forename: Hu Xue
authors.initials: HX
authors.lastname: Yu
authors.forename: Zejiao
authors.initials: Z
authors.lastname: Luo
keywords: Bioremediation; Gas Chromatography; Organophosphate pesticide; Profenofos
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