title: Molecular architecture of the ATP-dependent chromatin-remodeling complex SWR1.
abstract: The ATP-dependent chromatin-remodeling complex SWR1 exchanges a variant histone H2A.Z/H2B dimer for a canonical H2A/H2B dimer at nucleosomes flanking histone-depleted regions, such as promoters. This localization of H2A.Z is conserved throughout eukaryotes. SWR1 is a 1 megadalton complex containing 14 different polypeptides, including the AAA+ ATPases Rvb1 and Rvb2. Using electron microscopy, we obtained the three-dimensional structure of SWR1 and mapped its major functional components. Our data show that SWR1 contains a single heterohexameric Rvb1/Rvb2 ring that, together with the catalytic subunit Swr1, brackets two independently assembled multisubunit modules. We also show that SWR1 undergoes a large conformational change upon engaging a limited region of the nucleosome core particle. Our work suggests an important structural role for the Rvbs and a distinct substrate-handling mode by SWR1, thereby providing a structural framework for understanding the complex dimer-exchange reaction.
authors.affiliation: Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Vu Q
authors.initials: VQ
authors.lastname: Nguyen
authors.forename: Anand
authors.initials: A
authors.lastname: Ranjan
authors.forename: Florian
authors.initials: F
authors.lastname: Stengel
authors.forename: Debbie
authors.initials: D
authors.lastname: Wei
authors.forename: Ruedi
authors.initials: R
authors.lastname: Aebersold
authors.forename: Carl
authors.initials: C
authors.lastname: Wu
authors.forename: Andres E
authors.initials: AE
authors.lastname: Leschziner
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly; D004265:DNA Helicases / Q000737:chemistry* / Q000378:metabolism; D019281:Dimerization; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D009707:Nucleosomes / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism
chemical_list: D046912:Multiprotein Complexes; D009707:Nucleosomes; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000251:Adenosine Triphosphatases; C428198:RVB1 protein, S cerevisiae; C428199:RVB2 protein, S cerevisiae; C481261:Swr1 protein, S cerevisiae; D004265:DNA Helicases
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