title: Persistent overexpression of SERCA2a affects bladder functions under physiological conditions, but not in bladder outlet obstruction-induced sub-acute pathological conditions.
abstract: A functional impairment of the bladder and heart in a decompensated state caused by a pressure overload is accompanied by a decrease in the sarcoplasmic reticulum Ca(2+)-ATPase (SERCA2). The beneficial effects of SERCA2 overexpression in preserving cardiac functions have been previously reported. The aim of the present study was to investigate the effects of overexpressed SERCA2 on bladder functions under physiological and pathological conditions using partial bladder outlet obstruction (BOO) in SERCA2a transgenic Wistar rats (TG). Bladder cystometry and western blot analysis were performed using the wild-type Wistar rats (WT), TG, and BOO models (WTBOO and TGBOO). Persistent overexpression of SERCA2 induces reduced bladder compliance without hypertrophy in TG. BOO induces reduced bladder compliance and hypertrophy in WT and TG in the sub-acute phase, but persistent overexpression of SERCA2a in TG does not aggravate the bladder compliance and hypertrophy. In conclusion, SERCA2a overexpression affects bladder functions under physiological conditions, but not in BOO-induced sub-acute pathological conditions.
authors.affiliation: Department of Urology, Nara Medical University School of Medicine, 840 Shijo-cho, Kashihara, Nara, 634-8521, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamada
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Torimoto
authors.forename: Koji
authors.initials: K
authors.lastname: Obata
authors.forename: Akihide
authors.initials: A
authors.lastname: Hirayama
authors.forename: Kiyohide
authors.initials: K
authors.lastname: Fujimoto
authors.forename: Miyako
authors.initials: M
authors.lastname: Takaki
mesh_terms: D000208:Acute Disease; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006984:Hypertrophy / Q000209:etiology / Q000473:pathology; D051381:Rats; D055647:Rats, Transgenic; D017208:Rats, Wistar; D012519:Sarcoplasmic Reticulum / Q000378:metabolism; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D001743:Urinary Bladder / Q000473:pathology* / Q000503:physiopathology*; D001748:Urinary Bladder Neck Obstruction / Q000150:complications / Q000503:physiopathology*; D014554:Urination / Q000502:physiology
chemical_list: D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases
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