title: Association of CYBA rs7195830 polymorphism with estimated glomerular filtration rate in an adult Han sample from Jiangsu province, China.
abstract: BACKGROUND
  Reactive oxygen species are thought to contribute to the development of renal damage. The P22phox subunit of nicotinamide adenine dinucleotide phosphate (NAPDH) oxidase, encoded by the cytochrome b245a polypeptide gene, CYBA, plays a key role in superoxide anion production. We investigated the association of CYBA rs7195830 polymorphism with estimated glomerular filtration rate (eGFR) and the role it plays in the pathogenesis of chronic kidney disease (CKD) in a Han Chinese sample.
  
  
  METHODS
  The Gaoyou study enrolled 4473 participants. Serum levels of creatinine were measured and eGFR was estimated using the Chronic Kidney Disease Epidemiology Collaboration equations. The CYBA polymorphisms were genotyped. Then we investigated the association between eGFR and the rs7195830 polymorphism in the recessive model.
  
  
  RESULTS
  The AA genotype of rs7195830 was associated with significantly lower values of eGFR compared with the GG and AG genotypes ((102.76 ± 17.07) ml×min(-1)×1.73 m(-2) vs. (105.08 ± 16.30) ml×min(-1)± 1.73 m(-2)). The association remained significant in the recessive model after adjusting for age, gender, body mass index, smoking, hypertension, diabetes mellitus, uric acid, triglyceride, low density lipoprotein cholesterol and high density lipoprotein cholesterol (β=1.666, P=0.031). The rs7195832 AA genotype was an independent risk factor for CKD: eGFR <60 ml×min(-1)×1.73 m(-2) (odds ratio=3.32; 95% CI=1.21-9.13).
  
  
  CONCLUSIONS
  The AA genotype of rs7195830 is independently associated with lower estimated glomerular filtration rate and is significantly associated with CKD.
authors.affiliation: Department of Cardiology, First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, China; Department of Cardiology, Second Affiliated Hospital, Xuzhou Medical College, Xuzhou, Jiangsu 221000, China.
authors.forename: Kuan-Lu
authors.initials: KL
authors.lastname: Fan
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Zhang
authors.forename: Zhen-Yan
authors.initials: ZY
authors.lastname: Zhu
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Yao
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
authors.forename: Ning-Xia
authors.initials: NX
authors.lastname: Liang
authors.forename: Lei
authors.initials: L
authors.lastname: Gong
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Yin
authors.forename: Ke-Jiang
authors.initials: KJ
authors.lastname: Cao
authors.forename: Xin-Li
authors.initials: XL
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D005260:Female; D005919:Glomerular Filtration Rate / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D019255:NADPH Oxidases / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D051436:Renal Insufficiency, Chronic / Q000453:epidemiology / Q000235:genetics; D055815:Young Adult
chemical_list: D019255:NADPH Oxidases; C086986:CYBA protein, human