title: Pepper osmotin-like protein 1 (CaOSM1) is an essential component for defense response, cell death, and oxidative burst in plants.
abstract: Osmotin or osmotin-like protein, a PR-5 family member, is differentially induced in plants by abiotic and biotic stresses. Here, we demonstrate that the pepper (Capsicum annuum) osmotin-like protein 1 gene, CaOSM1, was required for the defense and hypersensitive cell death response and oxidative burst signaling during Xanthomonas campestris pv. vesicatoria (Xcv) infection. CaOSM1 protein was localized to the plasma membrane in leaf cells of Nicotiana benthamiana. Agrobacterium-mediated transient expression of CaOSM1 in pepper distinctly induced the hypersensitive cell death response and H2O2 accumulation. Knock-down of CaOSM1 in pepper by virus-induced gene silencing increased the susceptibility to Xcv infection, which was accompanied by attenuation of the cell death response and decreased accumulation of H2O2. CaOSM1 overexpression in transgenic Arabidopsis conferred reduced susceptibility and accelerated cell death response and H2O2 accumulation to infection by Pseudomonas syringe pv. tomato and Hyaloperonospora arabidopsidis. Together, these results suggest that CaOSM1 is involved in cell death and oxidative burst responses during plant defense against microbial pathogens.
authors.affiliation: Laboratory of Molecular Plant Pathology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-ku, Seoul, 136-713, Republic of Korea.|Department of Plant Biology and The Genome Center, College of Biological Sciences, University of California Davis, Davis, CA, 95616, USA.
authors.forename: Du Seok
authors.initials: du S
authors.lastname: Choi
authors.affiliation: Laboratory of Plant Pathology and Protection, Department of Horticulture, Gyeongnam National University of Science and Technology, Chilam-dong, Jinju, 660-758, Republic of Korea.
authors.forename: Jeum Kyu
authors.initials: JK
authors.lastname: Hong
authors.affiliation: Laboratory of Molecular Plant Pathology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-ku, Seoul, 136-713, Republic of Korea. bkhwang@korea.ac.kr.
authors.forename: Byung Kook
authors.initials: BK
authors.lastname: Hwang
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D002212:Capsicum / Q000235:genetics* / Q000378:metabolism* / Q000382:microbiology; D016923:Cell Death; D020868:Gene Silencing; D017343:Genes, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified; D044224:Pseudomonas syringae / Q000472:pathogenicity; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016897:Respiratory Burst; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D016959:Xanthomonas campestris / Q000472:pathogenicity
chemical_list: D010940:Plant Proteins; D011994:Recombinant Proteins; C053376:pathogenesis-related proteins, plant; D006861:Hydrogen Peroxide
keywords: Capsicum annuum; Cell death; Defense response; Osmotin-like protein; Pathogens; Transgenic Arabidopsis; Virus-induced gene silencing
references.citation: Plant Cell. 1992 Jun;4(6):645-56
references.pmid: 1392589
references.citation: Plant Cell. 1996 Oct;8(10):1773-91
references.pmid: 8914325
references.citation: Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):15107-11
references.pmid: 9844023
references.citation: Mol Cell. 2005 Jan 21;17(2):171-80
references.pmid: 15664187
references.citation: Plant Physiol. 1992 Sep;100(1):409-15
references.pmid: 16652977
references.citation: Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1888-92
references.pmid: 11607463
references.citation: Plant Cell. 1992 May;4(5):513-524
references.pmid: 12297654
references.citation: Plant J. 2011 May;66(4):642-55
references.pmid: 21299658
references.citation: Cell Host Microbe. 2008 Mar 13;3(3):126-35
references.pmid: 18329612
references.citation: Plant Mol Biol. 2000 Oct;44(3):321-34
references.pmid: 11199391
references.citation: Proc Natl Acad Sci U S A. 1997 Jun 24;94(13):7082-7
references.pmid: 9192695
references.citation: Plant Physiol. 1993 Mar;101(3):1113-4
references.pmid: 8310049
references.citation: J Mol Biol. 2000 Jul 21;300(4):1005-16
references.pmid: 10891285
references.citation: Plant Physiol. 2004 Sep;136(1):2818-30
references.pmid: 15347794
references.citation: Plant Mol Biol. 2004 Sep;56(1):15-27
references.pmid: 15604726
references.citation: Plant J. 1998 Dec;16(6):735-43
references.pmid: 10069079
references.citation: Plant Physiol. 2006 Jun;141(2):384-90
references.pmid: 16760492
references.citation: Plant Cell. 2011 Feb;23(2):823-42
references.pmid: 21335377
references.citation: Proteomics. 2004 Jun;4(6):1581-90
references.pmid: 15174128
references.citation: Plant Cell. 2011 May;23(5):1932-44
references.pmid: 21540435
references.citation: Plant Physiol. 2007 Nov;145(3):890-904
references.pmid: 17905862
references.citation: Plant Cell. 2012 Apr;24(4):1675-90
references.pmid: 22492811
references.citation: Plant Physiol. 2011 Aug;156(4):2011-25
references.pmid: 21628629
references.citation: Mol Cell. 2001 Oct;8(4):921-30
references.pmid: 11684026
references.citation: Plant J. 2004 Sep;39(6):920-32
references.pmid: 15341634
references.citation: Plant J. 2001 May;26(4):447-59
references.pmid: 11439131
references.citation: Plant Physiol. 2011 Jan;155(1):447-63
references.pmid: 21205632
references.citation: Plant Physiol. 2008 Nov;148(3):1695-706
references.pmid: 18790995
references.citation: Plant J. 2011 Jun;66(6):969-82
references.pmid: 21395887
references.citation: Plant J. 2003 Feb;33(4):621-32
references.pmid: 12609037
references.citation: Plant Cell. 1997 Jul;9(7):1157-1168
references.pmid: 12237381
references.citation: Mol Cell. 1998 May;1(6):807-17
references.pmid: 9660964
references.citation: Plant J. 2002 Sep;31(6):777-86
references.pmid: 12220268
references.citation: Genes Dev. 2002 Feb 15;16(4):490-502
references.pmid: 11850411
references.citation: Plant Cell. 1991 Jun;3(6):619-28
references.pmid: 1841721
references.citation: Annu Rev Phytopathol. 2006;44:135-62
references.pmid: 16602946
references.citation: Annu Rev Phytopathol. 2004;42:185-209
references.pmid: 15283665
references.citation: Plant Physiol. 1987 Oct;85(2):529-36
references.pmid: 16665731
references.citation: Plant Physiol. 1995 Jul;108(3):929-37
references.pmid: 7630973
references.citation: Plant Physiol. 1999 Aug;120(4):1137-46
references.pmid: 10444097
references.citation: Plant J. 2007 Sep;51(6):941-54
references.pmid: 17651371
references.citation: Plant Cell Physiol. 1997 Apr;38(4):441-8
references.pmid: 9177030
references.citation: Proc Natl Acad Sci U S A. 2001 Oct 9;98(21):12295-300
references.pmid: 11593047
references.citation: Cell Host Microbe. 2007 May 17;1(3):175-85
references.pmid: 18005697
references.citation: Proc Natl Acad Sci U S A. 1998 May 26;95(11):5857-64
references.pmid: 9600884
references.citation: Nature. 2001 Jun 14;411(6839):826-33
references.pmid: 11459065