title: Anticonvulsant and sedative effects of paederosidic acid isolated from Paederia scandens (Lour.) Merrill. in mice and rats.
abstract: This study was designed to evaluate the anticonvulsant and sedative effects of paederosidic acid isolated from Paederia scandens (Lour.) Merrill. in mice and rats. In the present study, anticonvulsant activities of paederosidic acid were evaluated by maximal electroshock and pentylenetetrazole-induced seizures in male mice. Then, pentobarbital sodium-induced sleeping time and locomotor activity tests in mice were used to evaluate the sedative effects of paederosidic acid. Finally, the mechanism of paederosidic acid was explored by evaluating the contents of Glu and GABA in the brain, and Western blot was used to measure GAD65 expression in the mouse brain. Paederosidic acid (5, 10, 20, and 40 mg/kg, ip) had significant anticonvulsant and sedative effects. Moreover, paederosidic acid increased brain gamma-aminobutyric acid and decreased glutamic acid in the brain, and it up-regulated expressions of GAD 65. In conclusion, our results suggest that paederosidic acid may be a promising future therapeutic agent for treatment of epilepsy.
authors.affiliation: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu 610083, PR China; Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.forename: Bin
authors.initials: B
authors.lastname: Kong
authors.forename: Jian-Wen
authors.initials: JW
authors.lastname: Gu
authors.forename: Yong-Qin
authors.initials: YQ
authors.lastname: Kuang
authors.forename: Lin
authors.initials: L
authors.lastname: Cheng
authors.forename: Wen-Tao
authors.initials: WT
authors.lastname: Yang
authors.forename: Jing-Min
authors.initials: JM
authors.lastname: Cheng
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ma
authors.forename: Xiao-Kun
authors.initials: XK
authors.lastname: Yang
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D005960:Glucosides / Q000494:pharmacology*; D005968:Glutamate Decarboxylase / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D006574:Heterocyclic Compounds, 2-Ring / Q000494:pharmacology*; D006993:Hypnotics and Sedatives / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019666:Rubiaceae / Q000737:chemistry*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D000927:Anticonvulsants; D005960:Glucosides; D006574:Heterocyclic Compounds, 2-Ring; D006993:Hypnotics and Sedatives; D010936:Plant Extracts; C000588809:paederosidic acid; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2
keywords: Anticonvulsant; Epilepsy; Paederia scandens; Paederosidic acid; Sedative