title: [Preparation of PVA-SA-PHB-AC composite carrier and m-cresol biodegradation by immobilized Lysinibacillus cresolivorans].
abstract: Due to the effects of outer environment and concentration limit on the biodegradation of m-cresol, a carrier with adsorption ability was synthesized. A PVA-SA-PHB-AC composite membrane was prepared by adding SA, PHB and AC into PVA immobilization carrier using the combination of freezing-thawing and boric acid methods. A highly-effective m-cresol-degrading strain Lysinibacillus cresolivorans was entrapped in it and the effects of structural properties such as micro-structure, stability and diffusion coefficient on m-cresol biodegradation were investigated. The results showed that PVA-SA-PHB-AC composite membrane had uniform pore opening, of which the average pore size, specific surface area, m-cresol adsorption capacity and diffusion coefficient was 33.68 nm, 15.30 m2 x g(-1), 3.86 mg x g(-1) and 5.62 x 10(-8) m2 x min(-1), respectively. It could be reused for more than two months, m-Cresol removal by immobilized L. cresolivorans was the coupling of adsorption and biodegradation, and the removal rate was jointly determined by mass-transfer rate and biodegradation rate. When the initial concentration of m-cresol was lower than 350 mg x L(-1), the mass-transfer rate of PVA-SA-PHB-AC was smaller than the biodegradation rate. The m-cresol removal rate depended on the mass-transfer rate, when the concentration was higher than 380 mg x L(-1), it was determined by the biodegradation rate. The addition of adsorbent could decrease the mass transfer coefficient in the carrier, while the higher concentration of substrate could be tolerated and the efficient biodegradation could be achieved in a wider range of concentrations. The biodegradation of m-cresol by immobilized microorganism showed that the modified carrier increased the reaction kinetics in a range of initial concentrations.
authors.affiliation: College of Environment and Energy, South China University of Technology, Guangzhou 510006, China. liting198801@163.com
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ren
authors.forename: Chao-Hai
authors.initials: CH
authors.lastname: Wei
mesh_terms: D000327:Adsorption; D000464:Alginates / Q000737:chemistry*; D001406:Bacillaceae / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D018914:Cells, Immobilized; D002606:Charcoal / Q000737:chemistry; D003408:Cresols / Q000302:isolation & purification* / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006885:Hydroxybutyrates / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000737:chemistry*; D000094122:Polyhydroxybutyrates
chemical_list: D000464:Alginates; D002606:Charcoal; D003408:Cresols; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006885:Hydroxybutyrates; D011091:Polyesters; D011142:Polyvinyl Alcohol; D062065:Wastewater; C032538:4-cresol; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates