title: Caffeic acid phenethyl ester (CAPE) protects against acute urogenital injury following pneumoperitoneum in the rat.
abstract: OBJECTIVE
  Our aim is to determine the biochemical and histologic changes induced in the kidneys, testis and prostate on possible ischemia and reperfusion (I/R) injury caused by pneumoperitoneum (PNP) in a rat model and to evaluate the ability of protective effects of caffeic acid phenethyl ester (CAPE).
  
  
  METHODS
  Twenty-eight adult male Wistar albino rats were randomly divided to one of three treatment groups, with seven animals in each group. Sham, laparoscopy (L), and laparoscopy plus CAPE (L + C) group were subjected to 60 min of PNP with 15 mmHg one hour before the desufflation period. Total oxidant status (TOS) and total antioxidant status (TAS) levels were determined in kidney, testis, and prostate. Kidney and testis tissues were removed to obtain a histologic score. Also, Johnsen scoring system was used for testicular tissue analysis.
  
  
  RESULTS
  L group had significantly higher TOS and lower TAS levels on kidney and testis compared to the other groups. In prostate biochemical analysis, there was not any difference between groups. No difference was found between groups according to kidney and testis tissues' histologic evaluation. In evaluation of Johnsen scoring, L group showed significant lower score compared to the other two groups.
  
  
  CONCLUSIONS
  Increased intraabdominal pressure (IAP) had an oxidative effect on kidney and testis but not on prostate in rats. Moreover, it could affect the testicular Johnsen score. All these adverse effects of IAP on both kidney and testis could be prevented by CAPE administration.
authors.affiliation: Department of Urology, Faculty of Medicine, University of Mustafa Kemal , Hatay , Turkey .
authors.forename: Murat Mehmet
authors.initials: MM
authors.lastname: Rifaioglu
authors.forename: Mursel
authors.initials: M
authors.lastname: Davarci
authors.forename: Ahmet
authors.initials: A
authors.lastname: Nacar
authors.forename: Harun
authors.initials: H
authors.lastname: Alp
authors.forename: Murat
authors.initials: M
authors.lastname: Celik
authors.forename: Nebihat Kaplan
authors.initials: NK
authors.lastname: Sefil
authors.forename: Mehmet
authors.initials: M
authors.lastname: Inci
mesh_terms: D058186:Acute Kidney Injury / Q000209:etiology* / Q000473:pathology / Q000517:prevention & control; D000818:Animals; D002109:Caffeic Acids / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical; D005832:Genital Diseases, Male / Q000209:etiology* / Q000473:pathology / Q000517:prevention & control; D010535:Laparoscopy / Q000009:adverse effects; D008297:Male; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000627:therapeutic use; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D013737:Testis / Q000293:injuries / Q000378:metabolism / Q000473:pathology
chemical_list: D002109:Caffeic Acids; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol