title: [Effects of bisphenol A on testis testosterone synthesis in mouse cultured in vitro].
abstract: OBJECTIVE
  To investigate the effects of BPA on testosterone synthesis and the related genes expression in the fetal testis of male mouse by organ culture in vitro.
  
  
  METHODS
  The testis tissues cultured in vitro were randomly divided into five groups, DMSO control group and 4 different concentration of BPA groups (10(-7), 10(-6), 10(-5) and 10(-4) mol/L respectively). The culture medium and gas were changed every 24 h. The histomorphological examination of the testis was conducted under optical microscope, the testosterone levels in the cultured medium were measured by radioimmunoassay, the expressions of related proteins were measured by immunohistochemistry, and the expression of 3betaHSD, P450scc, P450c17 and Vimentin were determined by real-time PCR.
  
  
  RESULTS
  Compared to the control group, the number of sertoli cells were decreased and flocculent changes appear in some Leydig ceils in 10(-) mol/L dose group. Testosterone secretion was gradually reduced as time goes on. The testosterone synthesis of 10(-5) mol/L dose group was increased in 48h (P < 0.05), and the rest groups were decreased, especially the 10(-4) mol/L dose group (P < 0.1). The expression of genes coding for proteins involved in testosterone biosynthesis, 3betaHSD, P450scc, P450c17 and Vimentin were reduced.
  
  
  CONCLUSIONS
  Bisphenol A can affect testosterone synthesis of mouse cultured in vitro through suppression of 3betaHSD ,P450scc, P450c17 and Vimentin.
authors.affiliation: West China School of Public Health Sichuan University, Chengdu 610041, China. penggehuang@163.com
authors.forename: Jie
authors.initials: J
authors.lastname: Huang
authors.forename: Hua
authors.initials: H
authors.lastname: Xu
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Gao
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Zhu
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D008297:Male; D051379:Mice; D009924:Organ Culture Techniques; D010636:Phenols / Q000633:toxicity*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013737:Testis / Q000187:drug effects / Q000378:metabolism*; D013739:Testosterone / Q000096:biosynthesis*; D000098826:Bisphenol A Compounds
chemical_list: D015096:3-Hydroxysteroid Dehydrogenases; D001559:Benzhydryl Compounds; D010636:Phenols; D012333:RNA, Messenger; D013739:Testosterone; C006780:bisphenol A; D000098826:Bisphenol A Compounds