title: A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis.
abstract: Sample preparation for Two-dimensional gel electrophoresis (2DE) is tedious and not sufficient to provide a comparative profile of secreted proteins for various strains of M. tuberculosis. High lipid content in mycobacteria limits the use of common methods as it can hinder the 2DE run. This study highlights the significance of SDS-TCA procedure over common used methods for the preparation of sample from culture filtrate as well as other proteinaceous fluids.
authors.affiliation: Department of Immunology, National JALMA Institute for Leprosy and Other Mycobacterial Diseases (ICMR) , Tajganj , Agra 282 001(India).
authors.forename: Gavish
authors.initials: G
authors.lastname: Kumar
authors.forename: Hari
authors.initials: H
authors.lastname: Shankar
authors.forename: Deepa
authors.initials: D
authors.lastname: Bisht
authors.forename: Prashant
authors.initials: P
authors.lastname: Sharma
authors.forename: Neelja
authors.initials: N
authors.lastname: Singhal
authors.forename: V M
authors.initials: VM
authors.lastname: Katoch
authors.forename: Beenu
authors.initials: B
authors.lastname: Joshi
keywords: Culture filtrate proteins; M. tuberculosis; SDS-TCA procedure; Two-dimensional gel electrophoresis
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