title: Identification of Mycobacterium bovis Isolates by a multiplex PCR.
abstract: Isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-PCR) targeting for RvD1Rv2031c and IS6110 sequences, specific for M. bovis and Mycobacterium tuberculosis complex respectively. The m-PCR successfully identified as M. bovis 88.24% of the isolates.
authors.affiliation: Universidade Federal do Rio de Janeiro , Rio de Janeiro, RJ , Brasil ; Universidade de Cuiabá , Cuiabá, MT , Brasil.
authors.forename: Eduardo Eustáquio
authors.initials: EE
authors.lastname: de Souza Figueiredo
authors.forename: Flávia Galindo
authors.initials: FG
authors.lastname: Silvestre
authors.forename: Wilma Neres
authors.initials: WN
authors.lastname: Campos
authors.forename: Leone Vinícius
authors.initials: LV
authors.lastname: Furlanetto
authors.forename: Luciana
authors.initials: L
authors.lastname: Medeiros
authors.forename: Walter
authors.initials: W
authors.lastname: Lilenbaum
authors.forename: Leila Sousa
authors.initials: LS
authors.lastname: Fonseca
authors.forename: Joab Trajano
authors.initials: JT
authors.lastname: Silva
authors.forename: Vânia Margaret Flosi
authors.initials: VM
authors.lastname: Paschoalin
keywords: Mycobacterium bovis; bovine tuberculosis; multiplex-PCR
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