title: Occurrence of multi-oocyte follicles in aquaporin 8-deficient mice.
abstract: BACKGROUND
  Granulosa cells play a key role in folliculogenesis and female reproduction. Our previous study demonstrated that water channel aquaporin-8 (AQP8) is expressed in mouse follicular granulosa cells and is an important determinant of granulosa cell apoptosis and follicular maturation. More roles of AQP8 in folliculogenesis remain to be determined.
  
  
  RESULTS
  The present study reports the increased occurrence of multi-oocyte follicles (MOFs) in ovaries of AQP8 knockout mice. The MOFs in AQP8-deficient ovaries contained two or three oocytes, and distributed at various follicle stages including primary (12.5%), secondary (50%), antral (18.8%) and atretic (18.8%) follicles in 5-week ovaries. The MOF is occasionally seen in wild-type ovary only in primary and secondary follicles. The number of MOFs in AQP8-deficient ovary reduced with age (26.7 +/- 5.2 per ovary at 5 weeks old, 14 +/- 5.5 at 10 weeks old, and 3.3 +/- 5.1 at 20 weeks old). mRNA expression of AQP5, AQP7, AQP8, AQP11 and AQP12 was detected in neonatal mouse ovaries and in granulosa cells in 4 week old mouse ovaries. The expression of AQP7, AQP11 and AQP12 mRNAs are decreased significantly in neonatal AQP8-deficient ovaries, whereas AQP5 mRNA expression remains unchanged.
  
  
  CONCLUSIONS
  The emergence of MOFs is associated with AQP8 deficiency. The study suggested the involvement of AQP8 in the formation of follicles and provided new insight into the molecular mechanisms of folliculogenesis.
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Dalian, P,R, China. math.jlu@gmail.com.
authors.forename: Weiheng
authors.initials: W
authors.lastname: Su
authors.forename: Xingang
authors.initials: X
authors.lastname: Guan
authors.forename: Di
authors.initials: D
authors.lastname: Zhang
authors.forename: Meiyan
authors.initials: M
authors.lastname: Sun
authors.forename: Longfei
authors.initials: L
authors.lastname: Yang
authors.forename: Fei
authors.initials: F
authors.lastname: Yi
authors.forename: Feng
authors.initials: F
authors.lastname: Hao
authors.forename: Xuechao
authors.initials: X
authors.lastname: Feng
authors.forename: Tonghui
authors.initials: T
authors.lastname: Ma
mesh_terms: D000818:Animals; D020346:Aquaporins / Q000235:genetics*; D002463:Cell Membrane Permeability / Q000235:genetics; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009865:Oocytes / Q000473:pathology*; D006080:Ovarian Follicle / Q000254:growth & development / Q000473:pathology*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D020346:Aquaporins; D012333:RNA, Messenger; C108330:aquaporin 8
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