title: Vibrio anguillarum bacterin uptake via the gills of Japanese flounder and subsequent immune responses.
abstract: The mucosal surfaces of fish allow for the introduction of foreign substances, including antigens, from the surrounding environment. In this study, uptake of Vibrio anguillarum J-O-3 serotype bacterin by Japanese flounder, and the subsequent immune responses were investigated. Immunohistochemistry revealed that the bacterin was taken up through the epithelial cells of gills. The transcription levels of inflammatory cytokines such as interleukin (IL)-1β, IL-6 and tumor necrosis factor α were significantly up-regulated in the gills at 3 days following exposure to the bacterin. There was also a corresponding increase in IL-8 receptor, CD4-1, CD4-2 and CD8α transcript levels in the gills. Our findings suggest that the gills play a major role in the uptake of V. anguillarum bacterin and induction of inflammation, which results in an activation of the adaptive immune response in teleost fish.
authors.affiliation: Tamaki Laboratory, National Research Institute of Aquaculture, Fisheries Research Agency, Tamaki, Mie 519-0423, Japan; The Japan Society for the Promotion of Science, 102-0083 Chiyoda, Tokyo, Japan. Electronic address: goshi540@affrc.go.jp.
authors.forename: Goshi
authors.initials: G
authors.lastname: Kato
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Takano
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Sakai
authors.forename: Tomomasa
authors.initials: T
authors.lastname: Matsuyama
authors.forename: Chihaya
authors.initials: C
authors.lastname: Nakayasu
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D017756:Aquaculture / Q000379:methods; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology*; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005432:Flounder / Q000276:immunology* / Q000382:microbiology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology*; D005880:Gills / Q000378:metabolism; D007150:Immunohistochemistry / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D014733:Vibrio / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D017931:DNA Primers
keywords: Immersion vaccine; Japanese flounder; Mucosal immunity; Paralichthys olivaceus; Vibrio anguillarum