title: Monocyte chemotactic protein-1 level in the aqueous humour as a prognostic factor for the outcome of trabeculectomy.
abstract: BACKGROUND
  The aim of this study was to elucidate the effects of the aqueous humour monocyte chemotactic protein-1 level on the surgical outcome of trabeculectomy in cases of phakic glaucoma.
  
  
  METHODS
  Retrospective study.
  
  
  METHODS
  Fifty-three cases of open-angle glaucoma.
  
  
  METHODS
  At the time of trabeculectomy, aqueous humour samples were collected before any incisions were made. The monocyte chemotactic protein-1 level was then determined by immunoassay. The Cox proportional hazards test was used to analyse the clinical factors related to failure of trabeculectomy. Subsequently, the success probability was calculated by Kaplan-Meier analysis and cumulative success probabilities were analysed by the log-rank test.
  
  
  METHODS
  Probability of success.
  
  
  RESULTS
  In 30 phakic glaucoma and 23 pseudophakic glaucoma cases, the mean monocyte chemotactic protein-1 levels in aqueous humour were 1165.2 and 2152.9 pg/mL, respectively; the difference was statistically significant (P < 0.0001). Univariate analysis showed that the outcomes of surgery were correlated with the monocyte chemotactic protein-1 level, but not with age, gender, diagnosis of exfoliation glaucoma, pseudophakic status or pretrabeculectomy intraocular pressure. Subsequent multivariate analysis revealed that only the aqueous humour monocyte chemotactic protein-1 level was significantly correlated with the outcomes of trabeculectomy (P = 0.043). Among the 30 phakic glaucoma eyes, the success probabilities after trabeculectomy were significantly different between the high and low monocyte chemotactic protein-1 groups (P = 0.018).
  
  
  CONCLUSIONS
  In open-angle glaucoma patients, the monocyte chemotactic protein-1 level is a prognostic factor for the results of trabeculectomy.
authors.affiliation: Department of Ophthalmology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Inoue
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kawaji
authors.forename: Hidenobu
authors.initials: H
authors.lastname: Tanihara
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001082:Aqueous Humor / Q000378:metabolism*; D018932:Chemokine CCL2 / Q000378:metabolism*; D005260:Female; D005902:Glaucoma, Open-Angle / Q000378:metabolism / Q000601:surgery*; D006801:Humans; D007118:Immunoassay; D007429:Intraocular Pressure; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D019591:Pseudophakia / Q000378:metabolism / Q000601:surgery; D012189:Retrospective Studies; D014130:Trabeculectomy; D016896:Treatment Outcome
chemical_list: D018932:Chemokine CCL2
keywords: MCP-1; aqueous humour; glaucoma; multiplex immunoassay; trabeculectomy