title: [An outbreak of imported dengue fever from Myanmar to the border of China, with its viral molecular epidemiological features].
abstract: OBJECTIVE
  To understand the epidemiologic characteristics of dengue fever, imported from Myanmar to the border of Yunnan province, China. Viral molecular epidemiologic features were also studied.
  
  
  METHODS
  Questionnaires were used on each diagnosed, suspected dengue fever, case or unknown cases with fever when coming from Myanmar entering the port and hospitals in Ruili city of Yunnan province. Serum samples of these patients were collected to detect IgM antibody against dengue virus and RT-PCR assay. Homology and phylogenetic tree based on the whole nucleotide sequence of PrM-C and NS5 gene of dengue virus were further analyzed.
  
  
  RESULTS
  A total of 103 sera were collected from patients at acute stage in Ruili city in July to November 2008. Among them, 49 cases were confirmed for dengue fever according to IgM and nucleic acid testings. Except one, other 48 cases were all imported into Ruili, from Myanmar. Of those, 18 patients were residents from Mujie city of Myanmar and hospitalized in Ruili and the rest 30 patients were Chinese citizens who had finished business and returned from Myanmar. Two isolates of serum samples from the imported cases were identified and both homology and phylogenetic analysis were performed, using the nucleotide sequences of PrM and NS5 genes. They were divided into dengue type 1 (RLB61) and dengue type 3 (RLC31) and were closer to the dengue virus strains isolated from Southeast Asia countries.
  
  
  CONCLUSIONS
  It is confirmed that an epidemic of dengue fever which was imported from Myanmar to Ruili city of Yunnan province, China. Evidence also showed that both type I and III epidemic strains of dengue virus did exist in Mujie city of Myanmar in 2008.
authors.affiliation: Yunnan Institute of Endemic Diseases Control and Prevention, Dali, China.
authors.forename: Hai-lin
authors.initials: HL
authors.lastname: Zhang
authors.forename: Shi-hong
authors.initials: SH
authors.lastname: Fu
authors.forename: Zhang
authors.initials: Z
authors.lastname: Deng
authors.forename: Jun
authors.initials: J
authors.lastname: Yuan
authors.forename: Hong-yue
authors.initials: HY
authors.lastname: Jiang
authors.forename: Ming-hua
authors.initials: MH
authors.lastname: Li
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Gao
authors.forename: Jing-lin
authors.initials: JL
authors.lastname: Wang
authors.forename: Yong-hua
authors.initials: YH
authors.lastname: Liu
authors.forename: Zheng-liu
authors.initials: ZL
authors.lastname: Yin
authors.forename: Wei-hong
authors.initials: WH
authors.lastname: Yang
authors.forename: Yu-zhen
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Yun
authors.initials: Y
authors.lastname: Feng
authors.forename: Huan-yu
authors.initials: HY
authors.lastname: Wang
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Liang
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D003715:Dengue / Q000453:epidemiology*; D003716:Dengue Virus / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D002052:Myanmar / Q000453:epidemiology; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics
chemical_list: D012367:RNA, Viral