title: Cattle immunized against the pathogenic L-α-glycerol-3-phosphate oxidase of Mycoplasma mycoides subs. mycoides fail to generate neutralizing antibodies and succumb to disease on challenge.
abstract: The membrane-associated enzyme L-α-glycerol-3-phosphate oxidase (GlpO) of Mycoplasma mycoides subs. mycoides (Mmm), the causal agent of contagious bovine pleuropneumonia (CBPP) has been identified as a virulence factor responsible for the release of toxic by-products such as H2O2 that mediate host cell injury. Since CBPP pathogenesis is based on host inflammatory reactions, we have determined the capacity of recombinant GlpO to generate in vivo protective responses against challenge in immunized cattle. We also investigated whether sera raised against recombinant GlpO in cattle and mice inhibit production of H2O2 by Mmm. Immunization of cattle with recombinant GlpO did not protect against challenge with a virulent strain of Mmm. Further, although both murine and bovine antisera raised against recombinant GlpO detected recombinant and native forms of GlpO in immunoblot assays with similar titres, only murine antibodies could neutralize GlpO enzymatic function. The data raise the possibility that Mmm has adapted to evade potential detrimental antibody responses in its definitive host.
authors.affiliation: International Livestock Research Institute, P.O. Box 30709, 00100 Nairobi, Kenya; Royal Veterinary College, University of London, United Kingdom. Electronic address: mum184@mail.usask.ca.
authors.forename: Musa M
authors.initials: MM
authors.lastname: Mulongo
authors.forename: Joachim
authors.initials: J
authors.lastname: Frey
authors.forename: Ken
authors.initials: K
authors.lastname: Smith
authors.forename: Christian
authors.initials: C
authors.lastname: Schnier
authors.forename: Hezron
authors.initials: H
authors.lastname: Wesonga
authors.forename: Jan
authors.initials: J
authors.lastname: Naessens
authors.forename: Declan
authors.initials: D
authors.lastname: McKeever
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D057134:Antibodies, Neutralizing / Q000276:immunology*; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control*; D005993:Glycerolphosphate Dehydrogenase / Q000276:immunology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D007106:Immune Sera / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009176:Mycoplasma mycoides / Q000201:enzymology* / Q000472:pathogenicity; D011002:Pleuropneumonia, Contagious / Q000276:immunology / Q000517:prevention & control*; D011994:Recombinant Proteins / Q000276:immunology; D037521:Virulence Factors / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D001428:Bacterial Vaccines; D007106:Immune Sera; D011994:Recombinant Proteins; D037521:Virulence Factors; D006861:Hydrogen Peroxide; D005993:Glycerolphosphate Dehydrogenase; C098831:glycerol-3-phosphate oxidase
keywords: Antibody; Cattle; Evolution; Mycoplasma; Vaccination; l-α-glycerol-3-phosphate oxidase
references.citation: Crit Rev Microbiol. 1997;23(4):269-354
references.pmid: 9439886
references.citation: Clin Diagn Lab Immunol. 2001 Jan;8(1):85-92
references.pmid: 11139200
references.citation: Vet Microbiol. 1998 Aug 28;63(1):13-28
references.pmid: 9810618
references.citation: Bull Epizoot Dis Afr. 1965 Jun;13:149-55
references.pmid: 14344194
references.citation: Vet J. 2007 Nov;174(3):513-21
references.pmid: 17157043
references.citation: Microbiology (Reading). 1994 Aug;140 ( Pt 8):2033-42
references.pmid: 7921253
references.citation: Vaccine. 2008 Apr 7;26(16):2010-9
references.pmid: 18342996
references.citation: Microbiology (Reading). 1999 Aug;145 ( Pt 8):2105-2116
references.pmid: 10463177
references.citation: Vet Res. 2009 Jul-Aug;40(4):35
references.pmid: 19364458
references.citation: Vet Res. 2006 Sep-Oct;37(5):733-44
references.pmid: 16820137
references.citation: J Bacteriol. 2005 Oct;187(19):6824-31
references.pmid: 16166545
references.citation: Clin Diagn Lab Immunol. 1996 Nov;3(6):746-52
references.pmid: 8914769
references.citation: Vet Immunol Immunopathol. 2008 Jul 15;124(1-2):192-7
references.pmid: 18406471
references.citation: J Mol Biol. 1990 Oct 5;215(3):403-10
references.pmid: 2231712
references.citation: Bioinformatics. 2010 Jul 1;26(13):1608-15
references.pmid: 20472543
references.citation: Vet Res. 2008 Jan-Feb;39(1):8
references.pmid: 18073095
references.citation: Vet Immunol Immunopathol. 2005 Sep 15;107(3-4):217-33
references.pmid: 15946743
references.citation: Int J Syst Evol Microbiol. 2009 Jun;59(Pt 6):1353-8
references.pmid: 19502315
references.citation: Physiol Rev. 2003 Apr;83(2):417-32
references.pmid: 12663864
references.citation: Rev Sci Tech. 1987 Sep;6(3):565-679
references.pmid: 32370343
references.citation: Ann N Y Acad Sci. 2000;916:71-80
references.pmid: 11193704
references.citation: Infect Immun. 2008 Jan;76(1):263-9
references.pmid: 17998309