title: Assessing the role of IL-35 in colorectal cancer progression and prognosis.
abstract: Despite the recent realization of Interleukin (IL)-35 in tumorigenesis, its exact impact on colorectal cancer (CRC) progression and prognosis, however, is yet to be elucidated clearly. We thus in the present report conducted comparative analysis of IL-35 levels between CRC patients and matched control subjects. IL-35 is highly expressed in all CRC tissues, which can be detected in vast majority of colorectal cancer cells. IL-35 levels in CRC lysates and serum samples are highly correlated to the severity of malignancy and the clinical stage of tumor. Particularly, a significant reduction for serum IL-35 was noted in patients after surgical resection, indicating that IL-35 promotes CRC progression associated with poor prognosis. Mechanistic study demonstrated a significant correlation between serum IL-35 levels and the number of peripheral regulatory T (Treg) cells in CRC patients, suggesting that IL-35 implicates in CRC pathogenesis probably by inducing Treg cells, while cancer cell-derived IL-35 may also recruit Treg cells into the tumor microenvironment in favor of tumor growth. Together, our data support that IL-35 could be a valuable biomarker for assessing CRC progression and prognosis in clinical settings.
authors.affiliation: Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical College Dongguan, China, 523808 ; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics Dongguan 523808, China.
authors.forename: Jin-Cheng
authors.initials: JC
authors.lastname: Zeng
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhang
authors.forename: Tian-Yu
authors.initials: TY
authors.lastname: Li
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Liang
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Wang
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Bao
authors.forename: Jun-Ai
authors.initials: JA
authors.lastname: Zhang
authors.forename: Wan-Dang
authors.initials: WD
authors.lastname: Wang
authors.forename: Wen-Yu
authors.initials: WY
authors.lastname: Xiang
authors.forename: Bin
authors.initials: B
authors.lastname: Kong
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Wang
authors.forename: Bin-Hua
authors.initials: BH
authors.lastname: Wu
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Chen
authors.forename: Long
authors.initials: L
authors.lastname: He
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.forename: Cong-Yi
authors.initials: CY
authors.lastname: Wang
authors.forename: Jun-Fa
authors.initials: JF
authors.lastname: Xu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood / Q000378:metabolism*; D016022:Case-Control Studies; D018797:Cell Cycle Proteins / Q000378:metabolism; D002634:Chemotaxis, Leukocyte; D003082:Colectomy; D015179:Colorectal Neoplasms / Q000097:blood / Q000276:immunology / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D018450:Disease Progression; D005260:Female; D006801:Humans; D007378:Interleukins / Q000097:blood / Q000378:metabolism*; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015778:Minor Histocompatibility Antigens; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism; D016896:Treatment Outcome; D059016:Tumor Microenvironment; D055815:Young Adult
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; C500695:CDCA5 protein, human; D018797:Cell Cycle Proteins; C097732:EBI3 protein, human; D007378:Interleukins; D015778:Minor Histocompatibility Antigens; C532417:interleukin-35, human
keywords: EBI3; IL-12p35; Interleukin-35; colorectal cancer; regulatory T cell
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