title: Rehydration capacities and rates for various porcine tissues after dehydration.
abstract: The biphasic effects of liquid on tissue biomechanics are well known in cartilage and vocal folds, yet not extensively in other tissue types. Past studies have shown that tissue dehydration significantly impacts biomechanical properties and that rehydration can restore these properties in certain tissue types. However, these studies failed to consider how temporal exposure to dehydrating or rehydrating agents may alter tissue rehydration capacity, as overexposure to dehydration may permanently prevent rehydration to the initial liquid volume. Select porcine tissues were dehydrated until they reached between 100% and 40% of their initial mass. Each sample was allowed to rehydrate for 5 hours in a 0.9% saline solution, and the percent change between the initial and rehydrated mass values was calculated. Spearman correlation tests indicated a greater loss in mass despite rehydration when tissues were previously exposed to greater levels of dehydration. Additionally, Pearson correlation tests indicated the total liquid mass of samples after complete rehydration decreased when previously exposed to higher levels of dehydration. Rehydration rates were found by dehydrating tissues to 40% of their initial mass followed by rehydration in a 0.9% saline solution for 60 minutes, with mass measurements occurring in 15 minute intervals. All tissues rehydrated nonlinearly, most increasing significantly in mass up to 30 minutes after initial soaking. This study suggests the ability for tissues to rehydrate is dependent on the level of initial dehydration exposure. In vitro rehydration experiments therefore require controlled dosage and temporal exposure to dehydrating and rehydrating agents to avoid incomplete rehydration, and caution should be taken when combining different tissue types in models of hydration.
authors.affiliation: University of Wisconsin-Madison School of Medicine and Public Health, Department of Surgery, Division of Otolaryngology - Head and Neck Surgery, Madison, Wisconsin, United States of America.
authors.forename: Jacob P
authors.initials: JP
authors.lastname: Meyer
authors.forename: Kieran E
authors.initials: KE
authors.lastname: McAvoy
authors.forename: Jack
authors.initials: J
authors.lastname: Jiang
mesh_terms: D000818:Animals; D003681:Dehydration / Q000503:physiopathology*; D005440:Fluid Therapy; D013552:Swine
references.citation: Acta Otolaryngol. 1992;112(1):151-9
references.pmid: 1575031
references.citation: J Bone Joint Surg Am. 1982 Jan;64(1):88-94
references.pmid: 7054208
references.citation: J Biomech. 1999 Sep;32(9):907-14
references.pmid: 10460127
references.citation: J Biomech. 1992 Aug;25(8):831-7
references.pmid: 1639827
references.citation: J Biomech. 2005 Aug;38(8):1636-42
references.pmid: 15958221
references.citation: J Biomech. 1998 Oct;31(10):927-34
references.pmid: 9840758
references.citation: J Biomech. 1997 Jan;30(1):79-81
references.pmid: 8970928
references.citation: J Orthop Res. 2001 Sep;19(5):845-51
references.pmid: 11562131
references.citation: Skin Res Technol. 2004 Nov;10(4):231-41
references.pmid: 15479446
references.citation: Otolaryngol Head Neck Surg. 2002 May;126(5):528-37
references.pmid: 12075228
references.citation: J Voice. 2011 Nov;25(6):657-62
references.pmid: 20951551
references.citation: J Biomech Eng. 1980 Feb;102(1):73-84
references.pmid: 7382457
references.citation: Lasers Surg Med. 1999;24(2):133-41
references.pmid: 10100651
references.citation: Ann Biomed Eng. 2003 Apr;31(4):482-91
references.pmid: 12723689