title: Long-Term Regeneration and Functional Recovery of a 15 mm Critical Nerve Gap Bridged by Tremella fuciformis Polysaccharide-Immobilized Polylactide Conduits.
abstract: Novel peripheral nerve conduits containing the negatively charged Tremella fuciformis polysaccharide (TF) were prepared, and their efficacy in bridging a critical nerve gap was evaluated. The conduits were made of poly(D,L-lactide) (PLA) with asymmetric microporous structure. TF was immobilized on the lumen surface of the nerve conduits after open air plasma activation. The TF-modified surface was characterized by the attenuated total reflection Fourier-transformed infrared spectroscopy and the scanning electron microscopy. TF modification was found to enhance the neurotrophic gene expression of C6 glioma cells in vitro. TF-modified PLA nerve conduits were tested for their ability to bridge a 15 mm gap of rat sciatic nerve. Nerve regeneration was monitored by the magnetic resonance imaging. Results showed that TF immobilization promoted the nerve connection in 6 weeks. The functional recovery in animals receiving TF-immobilized conduits was greater than in those receiving the bare conduits during an 8-month period. The degree of functional recovery reached ~90% after 8 months in the group of TF-immobilized conduits.
authors.affiliation: Institute of Polymer Science and Engineering, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan ; Rehabilitation Engineering Research Center, National Taiwan University, Taipei 10617, Taiwan ; Institute of Cellular and System Medicine, National Health Research Institutes, Miaoli County 35053, Taiwan.
authors.forename: Shan-Hui
authors.initials: SH
authors.lastname: Hsu
authors.forename: Shan-Ho
authors.initials: SH
authors.lastname: Chan
authors.forename: Chih-Tsung
authors.initials: CT
authors.lastname: Weng
authors.forename: Shu-Hui
authors.initials: SH
authors.lastname: Yang
authors.forename: Ching-Fen
authors.initials: CF
authors.lastname: Jiang
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