title: Protective effect of the probiotic Saccharomyces boulardii in Toxocara canis infection is not due to direct action on the larvae.
abstract: In a previous study our group found that the probiotic Saccharomyces boulardii was capable of reducing the intensity of infection in mice with toxocariasis. In order to assess whether the mechanism involved would be a direct action of the probiotic on Toxocara canis larvae, this study was designed. Both probiotics were singly cultivated in plates containing RPMI 1640 medium and T. canis larvae. S. boulardii and B. cereus var. toyoi cultures presented 97.6% and 95.7% of larvae with positive motility, respectively, and absence of color by the dye trypan blue, not representing significant difference to the control group (p > 0.05). We conclude that none of the probiotics showed in vitro effects on T. canis larvae and that the interaction with the intestinal mucosa is necessary for the development of the protective effect of S. boulardii.
authors.affiliation: Universidade Federal de Pelotas, 96010-900PelotasRSBrazilPost-Graduate Program in Parasitology, Universidade Federal de Pelotas (UFPEL); Campus Universitário, Caixa Postal 354, 96010-900 Pelotas, RS, Brazil. Tel.: 55.53.3275-7350, Fax: 55.53.3275-7350.
authors.forename: Luciana Farias da Costa de
authors.initials: LF
authors.lastname: Avila
authors.forename: Paula de Lima
authors.initials: Pde L
authors.lastname: Telmo
authors.forename: Lourdes Helena Rodrigues
authors.initials: LH
authors.lastname: Martins
authors.forename: Thaís Aimeé
authors.initials: TA
authors.lastname: Glaeser
authors.forename: Fabricio Rochedo
authors.initials: FR
authors.lastname: Conceição
authors.forename: Fábio Pereira Leivas
authors.initials: FP
authors.lastname: Leite
authors.forename: Carlos James
authors.initials: CJ
authors.lastname: Scaini
mesh_terms: D000818:Animals; D007814:Larva; D051379:Mice; D019936:Probiotics / Q000627:therapeutic use*; D012440:Saccharomyces / Q000737:chemistry*; D017166:Toxocara canis; D014120:Toxocariasis / Q000517:prevention & control*
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