title: Production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans 191.
abstract: This study concerned the production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans strain 191. In shaken flasks the maximum yield of chitinase was 6.9 U/mL after 72 h of cultivation at 25°C and 200 rpm. In a 5 L fermenter with 1.5 vvm aeration, the highest yield obtained was 4.19 U/mL after 168 h of fermentation at 25°C and 200 rpm, and using 3 vvm, it was 4.38 U/mL after 144 h of fermentation. The chitinase (61 KDa) was purified about 6.65 times by Sepharose CL 4B 200 gel filtration with a yield of 46.61%. The purified enzyme was able to lyse the cell walls of some fungi and to form protoplasts.
authors.affiliation: Laboratório de Bioquímica de Alimentos, Departamento de Ciências de Alimentos, Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas , Campinas, SP , Brasil.
authors.forename: Luciana F
authors.initials: LF
authors.lastname: Fleuri
authors.forename: Haroldo Y
authors.initials: HY
authors.lastname: Kawaguti
authors.forename: Hélia H
authors.initials: HH
authors.lastname: Sato
keywords: Cellulosimicrobium cellulans; chitinase; fungal lysis; protoplasts
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