title: Resistin affects lipid metabolism during adipocyte maturation of 3T3-L1 cells.
abstract: Resistin, an adipose-tissue-specific secretory factor, aggravates metabolic syndrome through impairment of glucose metabolism. Previously, we demonstrated that resistin expression was induced in both 3T3-L1 cells and primary pre-adipocytes derived from Zucker obese rats during the process of differentiation and maturation (Ikeda Y, Hama S, Kajimoto K, Okuno T, Tsuchiya H & Kogure K (2011) Biol Pharm Bull 34, 865-870). However, the biological function of resistin in adipocytes is poorly understood. In the present study, we examined the effects of resistin knockdown on the biological features of 3T3-L1 cells. We found that lipid content was significantly decreased in 3T3-L1 cells transfected with anti-resistin small interfering RNA (siRNA) after adipocyte differentiation. While expression of peroxisome proliferator activated receptor γ and CCAAT/enhancer-binding protein α was not affected, protein expression and transcriptional activity levels of carbohydrate response element binding protein (ChREBP), which upregulates transcription of lipogenic genes, decreased after anti-resistin siRNA treatment. Moreover, gene expression of fatty acid synthase and acetyl-CoA carboxylase 2, which are known to be regulated by ChREBP, were also suppressed by resistin knockdown. In contrast, activity of the fatty acid β-oxidation-regulating protein carnitine palmitoyltransferase 1 increased. These results suggest that resistin knockdown induces suppression of lipid production and activation of fatty acid β-oxidation. Consequently, resistin may affect lipid metabolism during adipocyte maturation.
authors.affiliation: Department of Biophysical Chemistry, Kyoto Pharmaceutical University, Japan.
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Ikeda
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsuchiya
authors.forename: Susumu
authors.initials: S
authors.lastname: Hama
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Kajimoto
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kogure
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics / Q000502:physiology; D000818:Animals; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D050556:Fatty Acid-Binding Proteins / Q000235:genetics; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D050356:Lipid Metabolism; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D052243:Resistin / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D022763:CCAAT-Enhancer-Binding Protein-alpha; C493411:Fabp4 protein, mouse; D050556:Fatty Acid-Binding Proteins; C506001:Mlxipl protein, mouse; D009687:Nuclear Proteins; D047495:PPAR gamma; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D052243:Resistin; C499623:Retn protein, mouse; C497388:Srebf1 protein, mouse; D051780:Sterol Regulatory Element Binding Protein 1; D014157:Transcription Factors
keywords: adipocyte; adipokine; lipid metabolism; lipogenic gene; resistin