title: Microwave-assisted green synthesis of ultrasmall fluorescent water-soluble silver nanoclusters and its application in chiral recognition of amino acids.
abstract: In this article, we established a fluorescent chiral recognition and detection method for cysteine based on the L-glutathione (L-GSH)-stabilized Ag nanoclusters (AgNCs) with high selectivity and sensitivity. A fast and green microwave (MW)-assisted strategy has been employed for synthesizing water-soluble fluorescent L-GSH-AgNCs. The reaction time was shortened from hours to several minutes. The synthetic process utilized L-glutathione as a stabilizing agent and a reducing agent without any other toxic reducing agent, such as NaBH4 or N2H4. The method is environmental friendly. Due to the different responses to AgNCs from chiral cysteine, we found d-cysteine hardly affects the fluorescence intensity of the AgNCs, whereas L-cysteine distinctly weakened its fluorescence intensity. This experiment indicated that fluorescence quenching efficiency of AgNCs was proportional to the concentration of L-cysteine in the range from 0.025 to 50 μmol L(-1).
authors.affiliation: Key Lab of Green Chemistry & Technology of MOE, College of Chemistry, Sichuan University, No. 29, Wangjiang Road, Chengdu, Sichuan 610064, China. lvy@scu.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Yingying
authors.initials: Y
authors.lastname: Su
authors.forename: Hongjie
authors.initials: H
authors.lastname: Song
authors.forename: Yi
authors.initials: Y
authors.lastname: Lv
mesh_terms: D000596:Amino Acids / Q000032:analysis / Q000737:chemistry; D003545:Cysteine / Q000032:analysis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D055772:Green Chemistry Technology / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008872:Microwaves; D012834:Silver / Q000737:chemistry*; D012995:Solubility; D013237:Stereoisomerism; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D000596:Amino Acids; D005456:Fluorescent Dyes; D014867:Water; D012834:Silver; D003545:Cysteine