title: Isolation and characterization of plant growth promoting bacteria from non-rhizospheric soil and their effect on cowpea (Vigna unguiculata (L.) Walp.) seedling growth.
abstract: The efficacy of four potential phosphate solubilizing Enterobacter isolated from non-rhizospheric soil in Western ghat forest in India. Plant growth promoting ability of these isolates was evaluated in cowpea. All are gram negative, rod shaped, 0.8-1.6 mm in size, and psychrotrophic in nature, grow from 5 to 40°C (optimum temp. 28 ± 2°C). All isolates exhibits growth at a wide range of pH 6-12, optimum at pH 7.0 and tolerates up to 7% (w/v) salt concentration. 16S rRNA gene sequencing reveals the confirmation of isolates to Enterobacter aerogenes sp. (NII-0907 and NII-0929), Enterobacter cloacae subsp. cloacae sp. (NII-0931) and Enterobacter asburiae sp. (NII-0934) with which they share >99% sequence similarity. Under in vitro conditions, all the four isolates were found to produce indole acetic acid, P-solubilization and hydrogen cyanide. The P-solubilizing activity coincided with a concomitant decrease in pH of the medium (pH 7.0-<3.0). The plant growth promotion properties were demonstrated through a cow pea (Vigna unguiculata (L.) walp) based bioassay under greenhouse conditions. Although the bacterial inoculation was found to result in significant increment in root, shoot and biomass and it stimulated bacterial counts in the rhizosphere. Hence, these isolates can further formulated and used for field application.
authors.affiliation: Biotechnology Division, National Institute of Interdisciplinary Science and Technology (CSIR), Industrial Estate, Pappanamcode, Trivandrum, 695019, India.
authors.forename: C K
authors.initials: CK
authors.lastname: Deepa
authors.forename: Syed G
authors.initials: SG
authors.lastname: Dastager
authors.forename: Ashok
authors.initials: A
authors.lastname: Pandey
references.citation: Curr Microbiol. 2009 Apr;58(4):378-83
references.pmid: 19067045
references.citation: Curr Opin Plant Biol. 2001 Feb;4(1):57-62
references.pmid: 11163169
references.citation: Crit Rev Microbiol. 1995;21(1):1-18
references.pmid: 7576148
references.citation: Antonie Van Leeuwenhoek. 2004 Jul;86(1):1-25
references.pmid: 15103234
references.citation: Curr Microbiol. 2001 Jul;43(1):51-6
references.pmid: 11375664
references.citation: Microbiol Res. 2005;160(2):127-33
references.pmid: 15881829
references.citation: J Appl Microbiol. 2004;96(3):473-80
references.pmid: 14962127
references.citation: An Acad Bras Cienc. 2006 Dec;78(4):791-807
references.pmid: 17143413
references.citation: Nucleic Acids Res. 2005 Jan 1;33(Database issue):D294-6
references.pmid: 15608200
references.citation: Antonie Van Leeuwenhoek. 2002 Aug;81(1-4):343-51
references.pmid: 12448732
references.citation: Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
references.pmid: 9396791
references.citation: J Mol Evol. 1980 Dec;16(2):111-20
references.pmid: 7463489
references.citation: Plant Physiol. 1951 Jan;26(1):192-5
references.pmid: 16654351
references.citation: Curr Microbiol. 2008 Jan;56(1):73-9
references.pmid: 17909886
references.citation: Mol Biol Evol. 1987 Jul;4(4):406-25
references.pmid: 3447015
references.citation: Brief Bioinform. 2004 Jun;5(2):150-63
references.pmid: 15260895
references.citation: Anal Biochem. 1987 Jan;160(1):47-56
references.pmid: 2952030
references.citation: Microbiol Res. 2007;162(1):69-76
references.pmid: 17140781
references.citation: J Am Mosq Control Assoc. 2008 Jun;24(2):219-27
references.pmid: 18666529
references.citation: Res Microbiol. 2008 Nov-Dec;159(9-10):635-42
references.pmid: 18996187