title: Halomonas olivaria sp. nov., a moderately halophilic bacterium isolated from olive-processing effluents.
abstract: A moderately halophilic, Gram-stain-negative, non-sporulating bacterium designed as strain TYRC17(T) was isolated from olive-processing effluents. The organism was a straight rod, motile by means of peritrichous flagella and able to respire both oxygen and nitrate. Growth occurred with 0-25 % (w/v) NaCl (optimum, 7 %), at pH 5-11 (optimum, pH 7.0) and at 4-50 °C (optimally at 35 °C). It accumulated poly-β-hydroxyalkanoate granules and produced exopolysaccharides. The predominant fatty acids were C18 : 1ω7c, C16 : 1ω7c and C16 : 0. Ubiquinone 9 (Q-9) was the only respiratory quinone. The DNA G+C content of TYRC17(T) was 53.9 mol%. Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia. TYRC17(T) showed high 16S-rRNA sequence identities in particular with the three last species listed (99.4-99.5 %). A multilocus sequence analysis (MLSA) using the 23S rRNA, gyrB, rpoD and secA genes allowed clarifying the phylogenetic position of TYRC17(T). This, combined with the level of DNA-DNA hybridization between TYRC17(T) and its closest relatives ranging from 21.6 % to 48.4 %, indicated that TYRC17(T) did not represent any of these species. On the basis of phenotypic and genotypic characteristics, and also genomic and phylogenetic evidence, it was concluded that strain TYRC17(T) represented a novel species of the genus Halomonas. The name Halomonas olivaria sp. nov. is proposed with TYRC17(T) ( = DSM 19074(T) = CCUG 53850B(T)) as the type strain.
authors.affiliation: Aix Marseille Université, CNRS/INSU, IRD, Mediterranean Institute of Oceanography (MIO), UM 110, 13288 Marseille.
authors.forename: Agnès
authors.initials: A
authors.lastname: Amouric
authors.affiliation: Aix Marseille Université, CNRS/INSU, IRD, Mediterranean Institute of Oceanography (MIO), UM 110, 13288 Marseille.
authors.forename: Pierre-Pol
authors.initials: PP
authors.lastname: Liebgott
authors.affiliation: Aix Marseille Université, CNRS/INSU, IRD, Mediterranean Institute of Oceanography (MIO), UM 110, 13288 Marseille.
authors.forename: Manon
authors.initials: M
authors.lastname: Joseph
authors.affiliation: Université de Lyon, Université Lyon 1, CNRS, UMR5558, Laboratoire de Biométrie et Biologie Evolutive, 43 Boulevard du 11 Novembre 1918, F-69622 Villeurbanne, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Brochier-Armanet
authors.affiliation: Aix Marseille Université, CNRS/INSU, IRD, Mediterranean Institute of Oceanography (MIO), UM 110, 13288 Marseille.
authors.forename: Jean
authors.initials: J
authors.lastname: Lorquin
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D001499:Bayes Theorem; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D020632:Halomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006885:Hydroxybutyrates / Q000378:metabolism; D008969:Molecular Sequence Data; D009018:Morocco; D058885:Multilocus Sequence Typing; D009693:Nucleic Acid Hybridization; D031658:Olea / Q000382:microbiology*; D010802:Phylogeny; D011091:Polyesters / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014451:Ubiquinone / Q000737:chemistry; D062065:Wastewater / Q000382:microbiology*; D000094122:Polyhydroxybutyrates
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006885:Hydroxybutyrates; D011091:Polyesters; D011135:Polysaccharides, Bacterial; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D062065:Wastewater; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C030571:ubiquinone 9