title: MicroRNA-130b targets Fmr1 and regulates embryonic neural progenitor cell proliferation and differentiation.
abstract: Fragile X syndrome, one of the most common forms of inherited mental retardation, is caused by expansion of the CGG repeat in the 5'-untranslated region of the X-linked Fmr1 gene, which results in transcriptional silencing and loss of expression of its encoded protein FMRP. The loss of FMRP increases proliferation and alters fate specification in adult neural progenitor cells (aNPCs). However, little is known about Fmr1 mRNA regulation at the transcriptional and post-transcriptional levels. In the present study, we report that miR-130b regulated Fmr1 expression by directly targeting its 3'-untranslated region (3' UTR). Up-regulation of miR-130b in mouse embryonic neural progenitor cells (eNPCs) decreased Fmr1 expression, markedly increased eNPC proliferation and altered the differentiation tendency of eNPCs, suggesting that antagonizing miR-130b may be a new therapeutic entry point for treating Fragile X syndrome.
authors.affiliation: State Key Laboratory of Food Science and Technology, College of Life Sciences and Food Engineering, Nanchang University, Nanchang 330047, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Gong
authors.forename: Kunshan
authors.initials: K
authors.lastname: Zhang
authors.forename: Yanlu
authors.initials: Y
authors.lastname: Wang
authors.forename: Junbang
authors.initials: J
authors.lastname: Wang
authors.forename: Yaru
authors.initials: Y
authors.lastname: Cui
authors.forename: Siguang
authors.initials: S
authors.lastname: Li
authors.forename: Yuping
authors.initials: Y
authors.lastname: Luo
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D049109:Cell Proliferation; D004622:Embryo, Mammalian; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000235:genetics* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D014162:Transfection; D015854:Up-Regulation
chemical_list: D020121:5' Untranslated Regions; D051860:Fragile X Messenger Ribonucleoprotein 1; D035683:MicroRNAs; C497684:Fmr1 protein, mouse; C582652:MIRN130 microRNA, mouse
keywords: Differentiation; Fmr1; Neural progenitor cells; Proliferation; miR-130b