title: Genetic and phenotypic selection affect natural (auto-) antibody reactivity of chickens.
abstract: Specificity, antibody isotype distribution and levels of natural antibodies (NAb) may be potential informative parameters for immune mediated natural disease resistance, immune modulation, and maintenance of physiological homeostasis. A large proportion of mammalian NAb have affinity for or are directed against self-antigens; so called natural auto antibodies (N(A)Ab). In the present study we showed the presence and typed levels and isotypes (total immunoglobulins, IgG and IgM) of N(A)Ab in plasma binding the 'auto-antigen' complex chicken liver cell lysate (CLL) of one-year old chickens from different genotype and phenotype backgrounds by ELISA and quantitative Western blotting. Higher levels of N(A)Ab binding CLL were found in plasma from chickens genetically selected for high specific antibody responses. In all birds, extensive staining patterns of plasma antibodies binding CLL were found for all isotypes, with IgG binding the highest number of CLL antigens and also showing the highest variation in staining patterns between individuals. Patterns of IgM antibodies binding CLL appeared to be more similar in all lines. Significant differences of binding patterns of N(A)Ab (antigen fragments of CLL and staining intensity) were detected between the different chicken lines, and lines could be clustered on the basis of their auto-antibody profile. In addition, also individual differences within lines were found. The present results indicate that analysis of the levels and the N(A)Ab repertoire of poultry like in mammals could provide a new way of distinguishing differences of immune competence and immune maturation between individuals, and could provide tools to select birds for health traits, or optimize hygiene and husbandry procedures.
authors.affiliation: Adaptation Physiology Group, Department of Animal Sciences, Wageningen University, Wageningen, The Netherlands.
authors.forename: Britt G
authors.initials: BG
authors.lastname: de Jong
authors.forename: Aart
authors.initials: A
authors.lastname: Lammers
authors.forename: Leonora A A
authors.initials: LA
authors.lastname: Oberendorf
authors.forename: Mike G B
authors.initials: MG
authors.lastname: Nieuwland
authors.forename: Huub F J
authors.initials: HF
authors.lastname: Savelkoul
authors.forename: Henk K
authors.initials: HK
authors.lastname: Parmentier
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D030161:Avian Proteins / Q000097:blood / Q000276:immunology; D001921:Brain / Q000276:immunology; D001947:Breeding; D002645:Chickens / Q000235:genetics / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008099:Liver / Q000276:immunology; D010641:Phenotype; D025341:Principal Component Analysis; D011485:Protein Binding
chemical_list: D001323:Autoantibodies; D030161:Avian Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M
references.citation: Autoimmun Rev. 2003 Sep;2(5):264-71
references.pmid: 12965177
references.citation: Proc Natl Acad Sci U S A. 2009 Aug 25;106(34):14484-9
references.pmid: 19667184
references.citation: J Clin Immunol. 1999 Jan;19(1):26-34
references.pmid: 10080102
references.citation: Nat Rev Genet. 2001 Apr;2(4):256-67
references.pmid: 11283698
references.citation: Poult Sci. 2011 Oct;90(10):2263-74
references.pmid: 21934009
references.citation: J Immunol. 1995 Jun 1;154(11):5769-78
references.pmid: 7751627
references.citation: Poult Sci. 2010 Feb;89(2):227-35
references.pmid: 20075273
references.citation: Eur J Immunol. 1997 Jun;27(6):1557-63
references.pmid: 9209510
references.citation: Crit Rev Immunol. 2004;24(5):297-320
references.pmid: 15663361
references.citation: Vaccine. 2000 Feb 25;18(16):1624-8
references.pmid: 10689139
references.citation: Immunol Today. 1991 May;12(5):154-9
references.pmid: 1715166
references.citation: Immunol Today. 2000 Dec;21(12):624-30
references.pmid: 11114423
references.citation: Trends Immunol. 2009 Jan;30(1):43-51
references.pmid: 19058756
references.citation: Eur J Immunol. 1984 Dec;14(12):1127-30
references.pmid: 6083871
references.citation: Springer Semin Immunopathol. 2005 Mar;26(4):347-62
references.pmid: 15633017
references.citation: Poult Sci. 2007 Jun;86(6):1090-9
references.pmid: 17495078
references.citation: Fish Shellfish Immunol. 2006 Oct;21(4):404-13
references.pmid: 16545579
references.citation: Mol Immunol. 2000 Dec;37(18):1141-9
references.pmid: 11451419
references.citation: Eur J Immunol. 1995 Sep;25(9):2598-604
references.pmid: 7589132
references.citation: PLoS One. 2013 Apr 02;8(4):e60726
references.pmid: 23589757
references.citation: J Dairy Sci. 2010 Nov;93(11):5467-73
references.pmid: 20965362
references.citation: Biomed Pharmacother. 2004 Jun;58(5):276-81
references.pmid: 15194162
references.citation: Immunol Rev. 1997 Dec;160:5-8
references.pmid: 9476660
references.citation: Dev Comp Immunol. 2010 Aug;34(8):821-7
references.pmid: 20230852
references.citation: Eur J Immunol. 1993 Nov;23(11):2851-9
references.pmid: 8223861
references.citation: Dev Comp Immunol. 2004 Jan;28(1):39-49
references.pmid: 12962981
references.citation: Scand J Immunol. 1996 Sep;44(3):243-51
references.pmid: 8795718
references.citation: J Immunol Methods. 2000 Jun 23;240(1-2):1-14
references.pmid: 10854596
references.citation: Clin Exp Immunol. 1975 Oct;22(1):84-90
references.pmid: 1212818
references.citation: Science. 1999 Dec 10;286(5447):2156-9
references.pmid: 10591647
references.citation: Science. 2010 Oct 29;330(6004):662-5
references.pmid: 21030656
references.citation: Cancer Invest. 1992;10(6):525-31
references.pmid: 1422890
references.citation: PLoS One. 2011 Mar 08;6(3):e17445
references.pmid: 21408156