title: Preliminary assessment of differential expression of candidate genes associated with atherosclerosis.
abstract: Identifying susceptible genes associated with the pathogenesis of atherosclerosis (ATH) may contribute toward better management of this condition. This preliminary study was aimed at assessing the expression levels of 11 candidate genes, namely tumor protein (TP53), transforming growth factor, beta receptor II (TGFBR2), cysthathionenine-beta-synthase (CBS), insulin receptor substrate 1 (IRS1), lipoprotein lipase (LPL), methylenetetrahydrofolate reductase (MTHFR), thrombomodulin (THBD), lecithin-cholesterol acyltransferase (LCAT), matrix metallopeptidase 9 (MMP9), low density lipoprotein receptor (LDLR), and arachidonate 5-lipoxygenase-activating protein (ALOX5AP) genes associated with ATH. Twelve human coronary artery tissues (HCATs) were obtained from deceased subjects who underwent post-mortem procedures. Six atherosclerotic coronary artery tissue (ACAT) samples representing the cases and non-atherosclerotic coronary artery tissue (NCAT) samples as controls were gathered based on predetermined inclusion and exclusion criteria. Gene expression levels were assessed using the GenomeLab Genetic Analysis System (GeXP). The results showed that LDLR, TP53, and MMP9 expression levels were significantly increased in ACAT compared to NCAT samples (p < 0.05). Thus, LDLR, TP53, and MMP9 genes may play important roles in the development of ATH in a Malaysian study population.
authors.affiliation: Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM).
authors.forename: Joan
authors.initials: J
authors.lastname: Blin
authors.forename: Zalinah
authors.initials: Z
authors.lastname: Ahmad
authors.forename: Lekhraj Rampal S O Gyanchand
authors.initials: LR
authors.lastname: Rampal
authors.forename: Norhafizah
authors.initials: N
authors.lastname: Mohtarrudin
authors.forename: Abdul Karim Haji
authors.initials: AK
authors.lastname: Tajudin
authors.forename: Rohayu Shahar
authors.initials: RS
authors.lastname: Adnan
mesh_terms: D000328:Adult; D003324:Coronary Artery Disease / Q000235:genetics*; D003331:Coronary Vessels / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics; D008296:Malaysia; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D000077294:Receptor, Transforming Growth Factor-beta Type II; D011973:Receptors, LDL / Q000235:genetics*; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: C526292:IRS1 protein, human; D055504:Insulin Receptor Substrate Proteins; C573412:LDLR protein, human; D011973:Receptors, LDL; D018125:Receptors, Transforming Growth Factor beta; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9