title: Pregnancy-associated genes contribute to antiluteolytic mechanisms in ovine corpus luteum.
abstract: The hypothesis that ovine luteal gene expression differs due to pregnancy status and day of estrous cycle was tested. RNA was isolated from corpora lutea (CL) on days 12 and 14 of the estrous cycle (NP) or pregnancy (P) and analyzed with the Affymetrix bovine microarray. RNA also was isolated from luteal cells on day 10 of estrous cycle that were cultured for 24 h with luteolytic hormones (OXT and PGF) and secretory products of the conceptus (IFNT and PGE2). Differential gene expression (>1.5-fold, P < 0.05) was confirmed using semiquantitative real-time PCR. Serum progesterone concentrations decreased from day 12 to day 15 in NP ewes (P < 0.05) reflecting luteolysis and remained >1.7 ng/ml in P ewes reflecting rescue of the CL. Early luteolysis (days 12-14) was associated with differential expression of 683 genes in the CL, including upregulation of SERPINE1 and THBS1. Pregnancy on day 12 (55 genes) and 14 (734 genes) also was associated with differential expression of genes in the CL, many of which were ISGs (i.e., ISG15, MX1) that were induced when culturing luteal cells with IFNT, but not PGE2. Finally, many genes, such as PTX3, IL6, VEGF, and LHR, were stabilized during pregnancy and downregulated during the estrous cycle and in response to culture of luteal cells with luteolytic hormones. In conclusion, pregnancy circumvents luteolytic pathways and activates or stabilizes genes associated with interferon, chemokine, cell adhesion, cytoskeletal, and angiogenic pathways in the CL.
authors.affiliation: Animal Reproduction and Biotechnology Laboratory, Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado;
authors.forename: Jared J
authors.initials: JJ
authors.lastname: Romero
authors.forename: Alfredo Q
authors.initials: AQ
authors.lastname: Antoniazzi
authors.forename: Natalia P
authors.initials: NP
authors.lastname: Smirnova
authors.forename: Brett T
authors.initials: BT
authors.lastname: Webb
authors.forename: Fang
authors.initials: F
authors.lastname: Yu
authors.forename: John S
authors.initials: JS
authors.lastname: Davis
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Hansen
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003338:Corpus Luteum / Q000166:cytology / Q000378:metabolism*; D015237:Dinoprost / Q000235:genetics / Q000378:metabolism; D015232:Dinoprostone / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D007370:Interferon Type I / Q000235:genetics / Q000378:metabolism; D003341:Luteolysis / Q000378:metabolism*; D010121:Oxytocin / Q000235:genetics / Q000378:metabolism; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics / Q000378:metabolism; D011270:Pregnancy, Animal / Q000235:genetics*; D011374:Progesterone / Q000097:blood; D034561:Sheep, Domestic / Q000502:physiology*; D015398:Signal Transduction; D013997:Time Factors
chemical_list: D007370:Interferon Type I; D011257:Pregnancy Proteins; C020947:interferon tau; D011374:Progesterone; D010121:Oxytocin; D015237:Dinoprost; D015232:Dinoprostone
keywords: corpus luteum; interferon; luteolysis; pregnancy; progesterone
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