title: UPLC-MS/MS method for determination of bepotastine in human plasma.
abstract: A sensitive and rapid method for quantitation of bepotastine in human plasma has been established using ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS). Valsartan was used as an internal standard. Bepotastine and internal standard in plasma sample were extracted using ethylacetate (liquid-liquid extraction). A centrifuged upper layer was then evaporated and reconstituted with the mobile phase of acetonitrile--5 mM ammonium formate (pH 3.5) (85:15, v/v). The reconstituted samples were injected into a phenyl column. Using MS/MS in the multiple reaction monitoring mode, bepotastine and valsartan were detected without severe interference from human plasma matrix. Bepotastine produced a protonated precursor ion ([M+H](+)) at m/z 389 and a corresponding product ion at m/z 167. And the internal standard produced a protonated precursor ion ([M+H](+)) at m/z 436 and a corresponding product ion at m/z 291. Detection of bepotastine in human plasma by the UPLC-ESI-MS/MS method was accurate and precise with a quantitation limit of 0.2 ng/mL. The validation, reproducibility, stability and recovery of the method were evaluated. The method has been successfully applied to pharmacokinetic studies of bepotastine in human plasma.
authors.affiliation: Korea Medicine Research Institute, Inc., Gyeonggi-do 462-120, South Korea College of Medicine, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, South Korea.
authors.forename: Yun Kyoung
authors.initials: YK
authors.lastname: Choi
authors.affiliation: College of Medicine, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, South Korea.
authors.forename: Yoon Hee
authors.initials: YH
authors.lastname: Chung
authors.affiliation: College of Medicine, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, South Korea.
authors.forename: Yun Sung
authors.initials: YS
authors.lastname: Nam
authors.affiliation: Korea Medicine Research Institute, Inc., Gyeonggi-do 462-120, South Korea.
authors.forename: Da Young
authors.initials: DY
authors.lastname: Kang
authors.affiliation: Korea Medicine Research Institute, Inc., Gyeonggi-do 462-120, South Korea.
authors.forename: Hohyun
authors.initials: H
authors.lastname: Kim
authors.affiliation: College of Medicine, Hanyang University, Seoul 133-791, South Korea.
authors.forename: Seo Eun
authors.initials: SE
authors.lastname: Lee
authors.affiliation: College of Medicine, Dankook University.
authors.forename: Hak Rim
authors.initials: HR
authors.lastname: Kim
authors.affiliation: Hallym University College of Medicine, Seoul 150-950, South Korea.
authors.forename: Yong Seong
authors.initials: YS
authors.lastname: Lee
authors.affiliation: College of Medicine, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, South Korea jhjeong3@cau.ac.kr.
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Jeong
mesh_terms: D000284:Administration, Oral; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D008297:Male; D010880:Piperidines / Q000097:blood* / Q000493:pharmacokinetics; D011725:Pyridines / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010880:Piperidines; D011725:Pyridines; C108476:bepotastine