title: Engineering of cofactor regeneration enhances (2S,3S)-2,3-butanediol production from diacetyl.
abstract: (2S,3S)-2,3-Butanediol ((2S,3S)-2,3-BD) is a potentially valuable liquid fuel and an excellent building block in asymmetric synthesis. In this study, cofactor engineering was applied to improve the efficiency of (2S,3S)-2,3-BD production and simplify the product purification. Two NADH regeneration enzymes, glucose dehydrogenase and formate dehydrogenase (FDH), were introduced into Escherichia coli with 2,3-BD dehydrogenase, respectively. Introduction of FDH resulted in higher (2S,3S)-2,3-BD concentration, productivity and yield from diacetyl, and large increase in the intracellular NADH concentration. In fed-batch bioconversion, the final titer, productivity and yield of (2S,3S)-2,3-BD on diacetyl reached 31.7 g/L, 2.3 g/(L·h) and 89.8%, the highest level of (2S,3S)-2,3-BD production thus far. Moreover, cosubstrate formate was almost totally converted to carbon dioxide and no organic acids were produced. The biocatalytic process presented should be a promising route for biotechnological production of NADH-dependent microbial metabolites.
authors.affiliation: 1] State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China [2] State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Lixiang
authors.initials: L
authors.lastname: Li
authors.forename: Cuiqing
authors.initials: C
authors.lastname: Ma
authors.forename: Chao
authors.initials: C
authors.lastname: Gao
authors.forename: Fei
authors.initials: F
authors.lastname: Tao
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
mesh_terms: D002072:Butylene Glycols / Q000378:metabolism*; D002384:Catalysis; D003931:Diacetyl / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005560:Formate Dehydrogenases / Q000378:metabolism; D015870:Gene Expression; D005798:Genes, Bacterial; D005800:Genes, Fungal; D009243:NAD / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D002072:Butylene Glycols; D009243:NAD; C026978:2,3-butylene glycol; D005560:Formate Dehydrogenases; D003931:Diacetyl
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