title: High sensitivity chemiluminescence enzyme immunoassay for detecting staphylococcal enterotoxin A in multi-matrices.
abstract: In this study, detection of staphylococcal enterotoxin A (SEA) in multi-matrices using a highly sensitive and specific microplate chemiluminescence enzyme immunoassay (CLEIA) has been established. A pair of monoclonal antibodies (mAbs) was selected from 37 anti-SEA mAbs by pairwise analysis, and the experimental conditions of the CLEIA were optimized. This CLEIA exhibited high performance with a wide dynamic range from 6.4 pg mL(-1) to 1600 pg mL(-1), and the measured low limit of detection (LOD) was 3.2 pg mL(-1). No cross-reactivity was observed when this method was applied to test SEB, SEC1, and SED. It has also been successfully applied for analyzing SEA in a variety of environmental, biological, and clinical matrices, such as sewage, tap water, river water, roast beef, peanut butter, cured ham, 10% nonfat dry milk, milk, orange juice, human urine, and serum. Thus, the highly sensitive and SEA-specific CLEIA should make it attractive for quantifying SEA in public health and diagnosis in near future.
authors.affiliation: Department of Immunology, The Fourth Military Medical University, No. 169 Changle West Road, Xi'an 710032, Shaanxi Province, PR China. Electronic address: hzcm1981@126.com.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Zhang
authors.forename: Zhijia
authors.initials: Z
authors.lastname: Liu
authors.forename: Yongming
authors.initials: Y
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.forename: Chaojun
authors.initials: C
authors.lastname: Song
authors.forename: Zhuwei
authors.initials: Z
authors.lastname: Xu
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yusi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.forename: Yuanjie
authors.initials: Y
authors.lastname: Sun
authors.forename: Lihua
authors.initials: L
authors.lastname: Chen
authors.forename: Liang
authors.initials: L
authors.lastname: Fang
authors.forename: Angang
authors.initials: A
authors.lastname: Yang
authors.forename: Kun
authors.initials: K
authors.lastname: Yang
authors.forename: Boquan
authors.initials: B
authors.lastname: Jin
mesh_terms: D000818:Animals; D002079:Butter / Q000382:microbiology; D002417:Cattle; D004768:Enterotoxins / Q000032:analysis* / Q000097:blood / Q000652:urine; D005516:Food Microbiology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D008460:Meat / Q000382:microbiology; D008892:Milk / Q000382:microbiology; D013210:Staphylococcus / Q000302:isolation & purification; D013552:Swine; D014871:Water Microbiology
chemical_list: D004768:Enterotoxins; C026066:enterotoxin A, Staphylococcal; D002079:Butter
keywords: CLEIA; Chemiluminescence enzyme immunoassay; Detection; Diagnosis; LOD; SEA; SEs; SFP; Sensitivity; Staphylococcal enterotoxin A; chemiluminescence enzyme immunoassay; detection of staphylococcal enterotoxin A; low limit of detection; mAbs; monoclonal antibodies; staphylococcal enterotoxins; staphylococcal food poisoning