title: Application of caged fluorescent nucleotides to live-cell RNA imaging.
abstract: A caged fluorescent nucleic acid probe, which contains a nucleotide modified with one photolabile nitrobenzyl unit and two hybridization-sensitive thiazole orange units, has been synthesized for area-specific fluorescence imaging of RNA in a cell. The probe emits very weak fluorescence regardless of the presence of the complementary RNA, whereas it shows hybridization-sensitive fluorescence emission after photoirradiation for uncaging. Such probes are generated via several chemical synthetic steps and are applicable to area-specific RNA imaging in a cell. Only probes that exist in the defined irradiation area are activated via uncaging irradiation.
authors.affiliation: The Research Center for Advanced Science and Technology (RCAST), The University of Tokyo, Tokyo, Japan.
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D055668:Photochemical Processes; D011804:Quinolines / Q000737:chemistry*; D059010:Single-Cell Analysis
chemical_list: D052160:Benzothiazoles; D015342:DNA Probes; D005456:Fluorescent Dyes; D011804:Quinolines; C051000:thiazole orange