title: Endo-MitoEGFP mice: a novel transgenic mouse with fluorescently marked mitochondria in microvascular endothelial cells.
abstract: Blood vessel-specific fluorescent transgenic mice are excellent tools to study the development of the vasculature and angiogenic processes. There is growing interest in the biological processes relevant to endothelial cells but limited tools exist to selectively evaluate subcellular functions of this cell type in vivo. Here, we report a novel transgenic animal model that expresses mitochondrially targeted enhanced green fluorescent protein (EGFP) via the Hb9 promoter, a homeobox transcription factor with limited known involvement in the vasculature. Random integration of the transgene, containing the entire mouse Hb9 promoter, was found to be expressed in a variety of vascularised tissues. Further inspection revealed that Mito-EGFP localizes to the endothelial cells (ECs) of a subset of microvascular blood vessels, especially in the central nervous system (CNS), heart, spleen, thymus, lymph nodes and skin. We demonstrate the utility of this novel transgenic mouse, named Endo-MitoEGFP, in the detection, imaging, and isolation of microvascular ECs and evaluation of EC mitochondrial function isolated from adult animals. These transgenic mice will be useful to studies of ECs in development, physiology, and pathology.
authors.affiliation: Centre d'Excellence en Neuromique de l'Université de Montréal, Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Université de Montréal, Montréal, Quebec, Canada ; Department of Biochemistry, Université de Montréal, Montréal, Quebec, Canada.
authors.forename: Sarah
authors.initials: S
authors.lastname: Pickles
authors.forename: Maxime
authors.initials: M
authors.lastname: Cadieux-Dion
authors.forename: Jorge I
authors.initials: JI
authors.lastname: Alvarez
authors.forename: Marc-Andre
authors.initials: MA
authors.lastname: Lécuyer
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Peyrard
authors.forename: Laurie
authors.initials: L
authors.lastname: Destroismaisons
authors.forename: Lydia
authors.initials: L
authors.lastname: St-Onge
authors.forename: Simone
authors.initials: S
authors.lastname: Terouz
authors.forename: Patrick
authors.initials: P
authors.lastname: Cossette
authors.forename: Alexandre
authors.initials: A
authors.lastname: Prat
authors.forename: Christine
authors.initials: C
authors.lastname: Vande Velde
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D055806:Microvessels / Q000166:cytology / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
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