title: Carbon disulfide exposure at peri-implantation disrupts embryo implantation by decreasing integrin β3 expression in the uterine tissue of pregnant mice.
abstract: Carbon disulfide (CS2) exposure might disrupt embryo implantation in females during pregnancy but the relevant mechanism remains unclear. Since integrin β3 has shown to be one of the cell adhesion molecules involved in embryo implantation, the current study examined the effect of CS2 exposure during the peri-implantation period on the protein and mRNA expression of integrin β3 and its DNA methylation degree in the uterine tissue of gestational mice. Exposure was on the 3rd day of gestation (GD3), GD4, GD5 and GD6, respectively, one time administration (631.4 mg/kg). The number of implanted embryos on GD9 was observed and compared with the control, it was decreased by 32.92%, 56.11%, 56.11% and 51.21%, respectively, which revealed that GD4 and GD5 were the most sensitive time for embryotoxicity. The protein and mRNA expression of integrin β3 were detected each day after exposure till the GD7 endpoint. It down-regulated the protein and mRNA expression of integrin β3 in uterine tissue and there was a positive correlation between the number of embryos and the expression of protein on the first and second day after exposure. However, the degree of integrin β3 DNA methylation was not affected, which was detected by bisulfite sequencing polymerase chain reaction (BSP) method. These findings suggest that the decreased protein and mRNA level of integrin β3 in the uterine tissue after mice exposure to CS2 might be relevant to the underlying mechanism of embryo implantation disorders, but DNA methylation of integrin β3 does not contribute to this action.
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Shandong University, 44 Wenhua Xi Road, Jinan, Shandong 250012, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Sun
authors.forename: Bingqin
authors.initials: B
authors.lastname: Dai
authors.forename: Yanling
authors.initials: Y
authors.lastname: Wu
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002246:Carbon Disulfide / Q000633:toxicity*; D002584:Cervix Uteri / Q000378:metabolism*; D019175:DNA Methylation; D010064:Embryo Implantation / Q000187:drug effects*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D005865:Gestational Age; D039661:Integrin beta3 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D039661:Integrin beta3; D012333:RNA, Messenger; D002246:Carbon Disulfide
keywords: BSP; CS(2); Carbon disulfide; DEPC; DNA methylation; Embryo implantation; GD; IOD; Integrin β(3); PMSF; RT-PCR; SDS; SDS–PAGE; bisulfite sequencing polymerase chain reaction; carbon disulfide; diethylpyrocarbonate; gestational day; integrated optical density; mRNA; phenylmethanesulfonyl fluoride; qRT-PCR; quantitative real-time polymerase chain reaction; reverse transcription-polymerase chain reaction; sodium dodecyl sulfonate; sodium dodecyl sulfonate–polyacrylamide gel electrophoresis