title: Bacterial community analysis of the water surface layer from a rice-planted and an unplanted flooded field.
abstract: The bacterial communities in floodwater, from a rice-planted and an unplanted field were characterized at the beginning (flooding stage) and at the end (harvest stage) of the rice cropping cycle. Most probable number estimations and plate counts of aerobic and anaerobic heterotrophic bacteria and of several metabolic bacterial groups (methanogens, sulfate-reducers, anaerobic sulfur and nonsulfur phototrophs, denitrifiers and ammonifiers) were similar in rice and unplanted floodwater at both sampling times. The analysis of denitrifiers and methanogens by fluorescent in situ hybridization revealed a shift in the phylogenetic affiliation only of the former group in the rice-planted floodwater. Terminal restriction fragment length polymorphism of 16S rRNA gene amplicons indicated that the bacterial communities of the rice-planted and unplanted soils were consistently diverse and strongly influenced by the season.
authors.affiliation: Cátedra de Microbiología, Facultad de Química y Facultad de Ciencias, Universidad de la República , Montevideo , Uruguay.
authors.forename: Ana Fernández
authors.initials: AF
authors.lastname: Scavino
authors.forename: Javier
authors.initials: J
authors.lastname: Menes
authors.forename: Lucía
authors.initials: L
authors.lastname: Ferrando
authors.forename: Silvana
authors.initials: S
authors.lastname: Tarlera
keywords: T-RFLP; floodwater; microbial community; rice
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