title: Correlation of embryonic skeletal muscle myotube physical characteristics with contractile force generation on an atomic force microscope-based bio-microelectromechanical systems device.
abstract: Rigorous analysis of muscle function inin vitrosystems is needed for both acute and chronic biomedical applications. Forces generated by skeletal myotubes on bio-microelectromechanical cantilevers were calculated using a modified version of Stoney's thin-film equation and finite element analysis (FEA), then analyzed for regression to physical parameters. The Stoney's equation results closely matched the more intensive FEA and the force correlated to cross-sectional area (CSA). Normalizing force to measured CSA significantly improved the statistical sensitivity and now allows for close comparison ofin vitrodata toin vivomeasurements for applications in exercise physiology, robotics, and modeling neuromuscular diseases.
authors.affiliation: NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Orlando, Florida 32826, USA.
authors.forename: K L
authors.initials: KL
authors.lastname: Pirozzi
authors.forename: C J
authors.initials: CJ
authors.lastname: Long
authors.forename: C W
authors.initials: CW
authors.lastname: McAleer
authors.forename: A S T
authors.initials: AS
authors.lastname: Smith
authors.forename: J J
authors.initials: JJ
authors.lastname: Hickman
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