title: The Lin28/let-7a/c-Myc pathway plays a role in non-muscle invasive bladder cancer.
abstract: We investigate the role of the Lin28/let-7a/c-Myc pathway in non-muscle invasive bladder cancer (NMIBC). Using RT-PCR, western blot and immunohistochemistry techniques, the levels of pre-let-7a, let-7a, Lin28 and c-Myc RNA and/or proteins were determined in samples of normal bladder tissue and bladder cancer. Expression of pre-let-7a was found to be negatively correlated with the pathological grade of bladder cancer, while let-7a showed a positive correlation with bladder cancer pathological grade. Expression of Lin28 RNA and protein was not significantly different between normal bladder tissue and low-grade transitional cell carcinoma of bladder (TCC) but the expression levels in high-grade TCC were remarkably increased. Expression of c-Myc RNA and protein was significantly higher in bladder cancer samples in comparison to normal bladder tissue without correlation with cancer differentiation. Expression of all the above RNAs and proteins showed no significant difference in Ta and T1 stages. The Lin28/let-7a/c-Myc pathway plays an important role in NMIBC. In particular, expression levels of let-7a correlate with the degree of cancer differentiation but not cancer stage.
authors.affiliation: Department of Urology, the First Affiliated Hospital, Jinan University, Avenue West 601, Guangzhou, Guangdong, 510630, People's Republic of China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Li
authors.forename: Haitao
authors.initials: H
authors.lastname: Liu
authors.forename: Caiyong
authors.initials: C
authors.lastname: Lai
authors.forename: Xinghua
authors.initials: X
authors.lastname: Du
authors.forename: Zexuan
authors.initials: Z
authors.lastname: Su
authors.forename: Shuangquan
authors.initials: S
authors.lastname: Gao
mesh_terms: D000368:Aged; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D008875:Middle Aged; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis* / Q000235:genetics; D016601:RNA-Binding Proteins / Q000032:analysis* / Q000235:genetics; D015398:Signal Transduction; D001743:Urinary Bladder / Q000378:metabolism / Q000473:pathology*; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000473:pathology*
chemical_list: C000715187:Lin28A protein, human; D035683:MicroRNAs; D016271:Proto-Oncogene Proteins c-myc; D016601:RNA-Binding Proteins; C487931:mirnlet7 microRNA, human