title: Pseudoalteromonas shioyasakiensis sp. nov., a marine polysaccharide-producing bacterium.
abstract: A novel exopolysaccharide-producing bacterium, designated strain SE3(T), was isolated from Pacific Ocean sediment. The strain was Gram-stain-negative, motile, strictly aerobic, oxidase-positive and catalase-positive, and required Na(+) for growth. Its major isoprenoid quinone was ubiquinone-8 (Q-8), and its cellular fatty acid profile mainly consisted of C16 : 1ω7c, C16 : 0 and C18 : 1ω7c. The DNA G+C content was 46.9 mol%. 16S rRNA gene sequence analysis suggested that strain SE3(T) is a member of the genus Pseudoalteromonas. Strain SE3(T) exhibited close phylogenetic affinity to Pseudoalteromonas arabiensis JCM 17292(T) (99.0 % 16S rRNA gene sequence similarity), Pseudoalteromonas lipolytica LMEB 39(T) (98.39 %) and Pseudoalteromonas donghaensis HJ51(T) (97.65 %). The DNA-DNA reassociation values between strain SE3(T) and P. arabiensis JCM 17292(T), P. lipolytica JCM 15903(T) and P. donghaensis LMG 24469(T) were 31, 26 and 44 %, respectively. Owing to the significant differences in phenotypic and chemotaxonomic characteristics, phylogenetic analysis based on 16S rRNA gene sequences and DNA-DNA relatedness data, the new isolate merits classification as a representative of novel species, for which the name Pseudoalteromonas shioyasakiensis is proposed. The type strain is SE3(T) ( = JCM 18891(T) = NCIMB 14852(T)).
authors.affiliation: School of Biological Science and Engineering, Tokai University, Minamisawa, Minami-ku, Sapporo 005-8601, Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Matsuyama
authors.affiliation: Graduate School of Science and Engineering, Tokai University, Minamisawa, Minami-ku, Sapporo 005-8601, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Sawazaki
authors.affiliation: School of Biological Sciences, Tokai University, Minamisawa, Minami-ku, Sapporo 005-8601, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Minami
authors.affiliation: School of Biological Sciences, Tokai University, Minamisawa, Minami-ku, Sapporo 005-8601, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kasahara
authors.affiliation: Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Horikawa
authors.affiliation: Bioprocess Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Yumoto
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010140:Pacific Ocean; D010802:Phylogeny; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D031161:Pseudoalteromonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011135:Polysaccharides, Bacterial; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8