title: Memory-enhancing effects of silk fibroin-derived peptides in scopolamine-treated mice.
abstract: Although enzyme-hydrolyzed silk fibroin has been reported to enhance cognitive function before, it has been still unknown which peptides can improve memory. Here we report that amino acid sequences of three novel peptides were identified from fibroin hydrolysate. Fibroin hydrolysate was obtained by hydrolysis with protease after partial hydrolysis with 5M CaCl2. Synthesized peptides derived from these sequences improved scopolamine-induced memory impairments in mice. We confirmed this hydrolysate had effects that improved learning and memory abilities by performing the Rey-Kim test. From this hydrolysate of silk fibroin, amino acid sequences of eight peptides were identified by LC-MS/MS. Three peptides (GAGAGTGSSGFGPY, GAGAGSGAGSGAGAGSGAGAGY, and SGAGSGAGAGSGAGAGSGA) were synthesized to investigate whether they could improve memory. Passive avoidance test and Morris water maze test were performed, and all peptides showed memory-enhancing abilities on scopolamine-induced memory impairments in mice. In this study, we identified three novel peptides that could improve memory, and that silk fibroin hydrolysate was a mixture of various active peptides that could enhance memory.
authors.affiliation: BrainOn Inc., Seoul 150-037, Republic of Korea.
authors.forename: Yong Koo
authors.initials: YK
authors.lastname: Kang
authors.forename: Woojoo
authors.initials: W
authors.lastname: Lee
authors.forename: Byunghoon
authors.initials: B
authors.lastname: Kang
authors.forename: Hannah
authors.initials: H
authors.lastname: Kang
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D005349:Fibroins / Q000302:isolation & purification* / Q000378:metabolism*; D006868:Hydrolysis; D008568:Memory / Q000187:drug effects*; D051379:Mice; D010447:Peptide Hydrolases / Q000378:metabolism; D010455:Peptides / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D012601:Scopolamine / Q000008:administration & dosage* / Q000009:adverse effects; D047011:Silk / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D010455:Peptides; D047011:Silk; D005349:Fibroins; D012601:Scopolamine; D010447:Peptide Hydrolases