title: Ofloxacin determination in urine, serum and pharmaceuticals using an automatic flow potentiometric system.
abstract: An automatic system was developed to determine ofloxacin in biological fluids and pharmaceutical formulations. Drug detection was carried out by a potentiometric membrane sensor based on [bis(trifluoromethyl)phenyl]borate as molecular-recognition material. The tubular shaped detector system was solidly attached to the manifold, creating a high-throughput stable setup (50 samples per hour) appropriate for routine antibiotic assessment. Under the optimized flow conditions, the sensor displayed a mean detection limit of 1 × 10(-5) M, a linear response over the concentrations of 2 × 10(-5) to 5 × 10(-3) M (slope of 57.4 mV decade(-1)) and a wide working pH range (2.1 - 6.6). The procedure was successfully applied to ofloxacin analysis in pharmaceuticals (relative deviation lower than 6%) and biological fluids at levels usually found after drug administration of clinical doses (recoveries between 91 and 106%). No significant interference from common excipients found in commercial formulations and inorganic ions usually present in biological fluids was noticed.
authors.affiliation: REQUIMTE, CEBIMED, Faculty of Health Sciences, University Fernando Pessoa.
authors.forename: Adriana M
authors.initials: AM
authors.lastname: Pimenta
authors.forename: M Renata S
authors.initials: MR
authors.lastname: Souto
authors.forename: Rita I L
authors.initials: RI
authors.lastname: Catarino
authors.forename: M Fernanda C
authors.initials: MF
authors.lastname: Leal
authors.forename: José L F Costa
authors.initials: JL
authors.lastname: Lima
mesh_terms: D001331:Automation / Q000295:instrumentation; D006801:Humans; D015394:Molecular Structure; D015242:Ofloxacin / Q000032:analysis / Q000097:blood* / Q000652:urine*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D011199:Potentiometry / Q000295:instrumentation
chemical_list: D004364:Pharmaceutical Preparations; D015242:Ofloxacin