title: Dynamics of human telomerase RNA structure revealed by antisense oligonucleotide technique.
abstract: Telomeres are the nucleoprotein complexes that cap the linear chromosome ends. Telomerase is a ribonucleoprotein that maintains telomere length in stem, embryonic and cancer cells. Somatic cells don't contain active telomerase and telomere function as mitotic clock and telomere length determines the number of cell divisions. Telomerase RNA (TER) contains the template for telomere synthesis and serves as a structural scaffold for holoenzyme assembly. We compared different oligonucleotide based methods for telomerase RNA inhibition, such as antisense oligonucleotides, knockdown by transient siRNA transfection and silencing by miRNA derived from short expressed RNA hairpin in HEK293 cells. All of these methods were applied to different TER regions. Our results revealed that CR2/CR3 domain of TER is accessible in vitro and in vivo and could serve as an optimal site for oligonucleotide-based telomerase silencing.
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: Daria V
authors.initials: DV
authors.lastname: Vasilkova
authors.forename: Dulat M
authors.initials: DM
authors.lastname: Azhibek
authors.forename: Timofei S
authors.initials: TS
authors.lastname: Zatsepin
authors.forename: Yulia V
authors.initials: YV
authors.lastname: Naraikina
authors.forename: Vladimir S
authors.initials: VS
authors.lastname: Prassolov
authors.forename: Maria M
authors.initials: MM
authors.lastname: Prokofjeva
authors.forename: Maria I
authors.initials: MI
authors.lastname: Zvereva
authors.forename: Maria P
authors.initials: MP
authors.lastname: Rubtsova
mesh_terms: D057809:HEK293 Cells; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D012313:RNA / Q000037:antagonists & inhibitors / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000235:genetics; D019098:Telomerase / Q000037:antagonists & inhibitors / Q000737:chemistry*; D016615:Telomere / Q000187:drug effects*
chemical_list: D016376:Oligonucleotides, Antisense; D034741:RNA, Small Interfering; C422952:telomerase RNA; D012313:RNA; D019098:Telomerase
keywords: Antisense oligonucleotide; RNA interference; Telomerase