title: A serum nuclear magnetic resonance-based metabolomic signature of advanced metastatic human breast cancer.
abstract: Breast cancer (BC) displays a high heterogeneity from histology to prognosis, metastatic evolution and treatment responses. We report here a (1)H NMR-based metabolic phenotyping study aiming at identifying coordinated metabolic serum changes associated with advanced metastatic breast cancer (MBC) in comparison to the localized early disease (EBC). A model discriminating EBC and MBC patients is obtained (n=85: 46 EBC and 39 MBC), and validated with an independent cohort (n=112: 61 EBC and 51 MBC; 89.8% sensitivity, 79.3% specificity). We identify 9 statistically significant metabolites involved in this discrimination: histidine, acetoacetate, glycerol, pyruvate, glycoproteins (N-acetyl), mannose, glutamate and phenylalanine. This work illustrates the strong potential of NMR metabolic phenotyping for the diagnosis, prognosis, and management of cancer patients.
authors.affiliation: Université de Lyon, CNRS/ENS Lyon/UCB-Lyon-1, Institut des Sciences Analytiques, Centre de RMN à Très Hauts Champs, 5 rue de la Doua, 69100 Villeurbanne, France; Université de Lyon, Centre Léon Bérard, Département d'Oncologie Médicale, 28 rue Laennec, 69008 Lyon, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Jobard
authors.affiliation: Université de Lyon, CNRS/ENS Lyon/UCB-Lyon-1, Institut des Sciences Analytiques, Centre de RMN à Très Hauts Champs, 5 rue de la Doua, 69100 Villeurbanne, France.
authors.forename: Clément
authors.initials: C
authors.lastname: Pontoizeau
authors.affiliation: Université de Lyon, CNRS/ENS Lyon/UCB-Lyon-1, Institut des Sciences Analytiques, Centre de RMN à Très Hauts Champs, 5 rue de la Doua, 69100 Villeurbanne, France.
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Blaise
authors.affiliation: Université de Lyon, Centre Léon Bérard, Département d'Oncologie Médicale, 28 rue Laennec, 69008 Lyon, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Bachelot
authors.affiliation: Université de Lyon, CNRS/ENS Lyon/UCB-Lyon-1, Institut des Sciences Analytiques, Centre de RMN à Très Hauts Champs, 5 rue de la Doua, 69100 Villeurbanne, France. Electronic address: benedicte.elena@ens-lyon.fr.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Elena-Herrmann
authors.affiliation: Université de Lyon, Centre Léon Bérard, Département d'Oncologie Médicale, 28 rue Laennec, 69008 Lyon, France. Electronic address: olivier.tredan@lyon.unicancer.fr.
authors.forename: Olivier
authors.initials: O
authors.lastname: Trédan
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D001943:Breast Neoplasms / Q000097:blood* / Q000473:pathology*; D015331:Cohort Studies; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D015999:Multivariate Analysis; D009362:Neoplasm Metastasis; D010641:Phenotype; D011379:Prognosis; D012372:ROC Curve; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor
keywords: Breast cancer; Metabolomics; Metabonomics; Metastatic; NMR; Serum