title: In vivo optical coherence tomography of light-driven melanosome translocation in retinal pigment epithelium.
abstract: Optical coherence tomography (OCT) may revolutionize fundamental investigation and clinical management of age-related macular degeneration and other eye diseases. However, quantitative OCT interpretation is hampered due to uncertain sub-cellular correlates of reflectivity in the retinal pigment epithelium (RPE) and photoreceptor. The purpose of this study was twofold: 1) to test OCT correlates in the RPE, and 2) to demonstrate the feasibility of longitudinal OCT monitoring of sub-cellular RPE dynamics. A high resolution OCT was constructed to achieve dynamic imaging of frog eyes, in which light-driven translocation of RPE melanosomes occurred within the RPE cell body and apical processes. Comparative histological examination of dark- and light-adapted eyes indicated that the RPE melanin granule, i.e., melanosome, was a primary OCT correlate. In vivo OCT imaging of RPE melanosomes opens the opportunity for quantitative assessment of RPE abnormalities associated with disease, and enables longitudinal investigation of RPE kinetics correlated with visual function.
authors.affiliation: Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294.
authors.forename: Qiu-Xiang
authors.initials: QX
authors.lastname: Zhang
authors.forename: Rong-Wen
authors.initials: RW
authors.lastname: Lu
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Messinger
authors.forename: Christine A
authors.initials: CA
authors.lastname: Curcio
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Guarcello
authors.forename: Xin-Cheng
authors.initials: XC
authors.lastname: Yao
mesh_terms: D000221:Adaptation, Ocular / Q000502:physiology; D000818:Animals; D001692:Biological Transport; D008027:Light; D020460:Melanosomes / Q000378:metabolism*; D011894:Rana pipiens; D055213:Retinal Pigment Epithelium / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D041623:Tomography, Optical Coherence
references.citation: Invest Ophthalmol Vis Sci. 2006 Dec;47(12):5522-8
references.pmid: 17122144
references.citation: Ophthalmology. 2012 Nov;119(11):2203-2204.e1
references.pmid: 23122463
references.citation: Opt Express. 2009 Nov 23;17(24):21634-51
references.pmid: 19997405
references.citation: Prog Brain Res. 2001;131:477-85
references.pmid: 11420964
references.citation: Invest Ophthalmol Vis Sci. 2010 Apr;51(4):2149-57
references.pmid: 19797228
references.citation: Invest Ophthalmol Vis Sci. 2011 Jun 06;52(7):3943-54
references.pmid: 21421869
references.citation: Nat Med. 2001 Apr;7(4):502-7
references.pmid: 11283681
references.citation: J Invest Dermatol. 1995 Jun;104(6):946-52
references.pmid: 7769264
references.citation: Mol Aspects Med. 2012 Aug;33(4):295-317
references.pmid: 22542780
references.citation: Physiology (Bethesda). 2010 Feb;25(1):8-15
references.pmid: 20134024
references.citation: Retina. 2001;21(1):57-61
references.pmid: 11217931
references.citation: J Biomed Opt. 2012 Mar;17(3):036010
references.pmid: 22502568
references.citation: Invest Ophthalmol Vis Sci. 1985 Dec;26(12):1659-94
references.pmid: 2933359
references.citation: Retina. 2011 Sep;31(8):1609-19
references.pmid: 21844839
references.citation: J Biomed Opt. 2002 Jul;7(3):457-63
references.pmid: 12175297
references.citation: Neurosurgery. 1998 Oct;43(4):834-41
references.pmid: 9766311
references.citation: Vis Neurosci. 2005 Mar-Apr;22(2):203-9
references.pmid: 15935112
references.citation: Arch Ophthalmol. 2004 Apr;122(4):477-85
references.pmid: 15078664
references.citation: J Photochem Photobiol B. 1992 Feb 28;12(3):215-58
references.pmid: 1635010
references.citation: Arch Ophthalmol. 2004 Apr;122(4):564-72
references.pmid: 15078675
references.citation: Opt Express. 2008 Oct 13;16(21):16410-22
references.pmid: 18852747
references.citation: Exp Eye Res. 1989 May;48(5):589-603
references.pmid: 2786817
references.citation: IEEE Trans Med Imaging. 2001 Sep;20(9):900-16
references.pmid: 11585207
references.citation: Eye (Lond). 1990;4 ( Pt 2):326-32
references.pmid: 2199240
references.citation: Science. 1991 Nov 22;254(5035):1178-81
references.pmid: 1957169
references.citation: Invest Ophthalmol Vis Sci. 2013 May 01;54(5):3721-8
references.pmid: 23633665
references.citation: Invest Ophthalmol Vis Sci. 1983 Sep;24(9):1203-10
references.pmid: 6885308
references.citation: Lasers Surg Med. 2007 Oct;39(9):687-95
references.pmid: 17960754
references.citation: Bull World Health Organ. 2004 Nov;82(11):844-51
references.pmid: 15640920
references.citation: Opt Lett. 1995 Mar 15;20(6):524-6
references.pmid: 19859243
references.citation: Ophthalmic Surg Lasers Imaging Retina. 2013 Mar-Apr;44(2):208-10
references.pmid: 23413892
references.citation: Opt Express. 2004 May 31;12(11):2404-22
references.pmid: 19475077
references.citation: Pharmacol Rep. 2005 Jul-Aug;57(4):467-74
references.pmid: 16129913
references.citation: Physiol Rev. 2005 Jul;85(3):845-81
references.pmid: 15987797
references.citation: J Cell Sci Suppl. 1993;17:189-95
references.pmid: 8144697
references.citation: Technol Cancer Res Treat. 2003 Dec;2(6):525-35
references.pmid: 14640764
references.citation: Invest Ophthalmol Vis Sci. 2011 Dec 28;52(13):9703-9
references.pmid: 22110066
references.citation: Invest Ophthalmol Vis Sci. 1983 Jan;24(1):1-15
references.pmid: 6826305
references.citation: Biomed Opt Express. 2012 Jul 1;3(7):1670-83
references.pmid: 22808437
references.citation: Invest Ophthalmol Vis Sci. 2012 Dec 13;53(13):8139-45
references.pmid: 23150616