title: Utility of cryopreserved hepatocytes suspended in serum to predict hepatic clearance in dogs and monkeys.
abstract: An in vitro-in vivo correlation analysis between observed and predicted metabolic clearance in multiple preclinical species including dogs and monkeys constitutes an integral part of prediction for the pharmacokinetics in humans by using liver-derived in vitro preparations. Empirical values of the scaling factor for the extrapolation of metabolic (intrinsic) clearance in the in vitro preparation to that for whole liver were calculated for each preparation of 8 and 5 cryopreserved dog and monkey hepatocytes, respectively, by optimizing the objective function of average fold error between predicted and observed metabolic (intrinsic) clearance for eight and 11 standard compounds for dogs and monkeys, respectively. Thus obtained values of the scaling factor ranged from 5.46 × 10(9) to 19.9 × 10(9) cells/kg body weight with an average of 10.3 × 10(9) cells/kg body weight in dogs, and the value ranged from 2.36 × 10(9) to 4.21 × 10(9) cells/kg body weight with an average of 3.17 × 10(9) cells/kg body weight in monkeys, which were both consistent with biologically calculated values in corresponding species. These results demonstrated the utility of commercially available cryopreserved preparations of dog and monkey hepatocytes for the in vitro-in vivo correlation analyses with the aid of empirically or biologically obtained scaling factors at the early development stage of new drug candidates.
authors.affiliation: Discovery Drug Metabolism & Pharmacokinetics, Pharmacokinetics Research Laboratories, Tsukuba Research Center, Taiho Pharmaceutical Co., Ltd.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Shibata
authors.forename: Jiro
authors.initials: J
authors.lastname: Kuze
authors.forename: Masato
authors.initials: M
authors.lastname: Chiba
mesh_terms: D000818:Animals; D001835:Body Weight; D015925:Cryopreservation; D004285:Dogs; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D008252:Macaca fascicularis; D008297:Male; D008657:Metabolic Clearance Rate; D008954:Models, Biological; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D010599:Pharmacokinetics; D044967:Serum / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D004364:Pharmaceutical Preparations