title: Establishing the reference range for T lymphocytes subpopulations in adults and children from Brazil.
abstract: In Brazil, the existing reference values for T-lymphocytes subsets are based on data originated in other countries. There is no local information on normal variation for these parameters in Brazilian adults and children. We evaluated the normal variation found in blood donors from five large Brazilian cities, in different regions, and in children living in Salvador, and Rio de Janeiro. All samples were processed by flow cytometry. The results were analyzed according to region, gender, and lifestyle of blood donors. A total of 641 adults (63% males), and 280 children (58% males) were involved in the study. The absolute CD3+, and CD4+ cells count were significantly higher for females (adults and children). Higher CD4+ cell count in adults was associated with smoking, while higher CD8+ count was found among female children. Higher counts, for all T-cells subsets, were detected in blood donors from southeast / south regions while those living in the northern region had the lowest values. Individuals from midwestern and northeastern regions had an intermediate count for all these cells subsets. However, these differences did not reach statistical significance. In Brazil, gender and smoking, were the main determinants of differences in T-lymphocytes reference values.
authors.affiliation: Federal University of Bahia, SalvadorBA, Brazil.
authors.forename: Alex José Leite
authors.initials: AJ
authors.lastname: Torres
authors.forename: Ana Luiza Dias
authors.initials: AL
authors.lastname: Angelo
authors.forename: Márcio Oliveira
authors.initials: MO
authors.lastname: Silva
authors.forename: Milena de Carvalho
authors.initials: Mde C
authors.lastname: Bastos
authors.forename: Denise Ferreira de
authors.initials: DF
authors.lastname: Souza
authors.forename: Lílian Amaral
authors.initials: LA
authors.lastname: Inocêncio
authors.forename: José Alexandre Rodrigues de
authors.initials: JA
authors.lastname: Lemos
authors.forename: Ruy S
authors.initials: RS
authors.lastname: Junior
authors.forename: Andréa Cauduro de
authors.initials: AC
authors.lastname: Castro
authors.forename: Patrícia Vianna Bonnini
authors.initials: PV
authors.lastname: Palma
authors.forename: Loredana
authors.initials: L
authors.lastname: Ceci
authors.forename: Eduardo Martins
authors.initials: EM
authors.lastname: Netto
authors.forename: Carlos
authors.initials: C
authors.lastname: Brites
mesh_terms: D000328:Adult; D000367:Age Factors; D001782:Blood Donors; D001938:Brazil; D002648:Child; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology; D008297:Male; D012016:Reference Values
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