title: Effects of Metoclopramide and Ranitidine on survival of flat template McFarlane skin flaps in a rat wound healing model.
abstract: Wound healing re-provides the morphological integrity after trauma. We investigated the effects of Metoclopramide and Ranitidine on survival of flat template McFarlane skin flaps in an experimental wound healing model.Rats (n:32) were randomly allocated in following groups: Flap control (Control), Metoclopramide(MET), Ranitidine(RAN) and Metoclopramide+Ranitidine (MET+RAN). After flap elevation, ip 10 mg/kg Ranitidin or 5 mg/kg Metoclopramide or the combination of both drugs were administered for 3 days. Next analgesia was maintained. No additional drugs were used for controls. On 10th day, whole cut skin flaps were excised, fixed in buffered formaldehyde and processed with histological techniques. Paraffine sections were stained with Hematoxylen-Eosin, Mallory-Azan and immunohistochemically with Desmin and Fibronectin and then evaluated with light microscopy.Experimental groups showed differences for epidermal degeneration, edema, hypertrophy of the hair follicles, neutrophil infiltration and areolar degeneration. Metoclopramide or Ranitidine administration positively impacts wound healing.This unique study emphasizes the importance of considering Metoclopramide or Ranitidine for possible adverse effects on flap survival in surgical clinics, therefore the combination of both drugs is not more effective.
authors.affiliation: Histology and Embryology, Ege University Faculty of Medicine, Izmir, Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Yilmaz Dilsiz
authors.affiliation: Plastic and Reconstructive Surgery, Ege University Faculty of Medicine, Izmir, Turkey.
authors.forename: I
authors.initials: I
authors.lastname: Akhundzada
authors.affiliation: Plastic and Reconstructive Surgery, Ege University Faculty of Medicine, Izmir, Turkey.
authors.forename: U
authors.initials: U
authors.lastname: Bilkay
authors.affiliation: Histology and Embryology, Ege University Faculty of Medicine, Izmir, Turkey.
authors.forename: Y
authors.initials: Y
authors.lastname: Uyanikgil
authors.affiliation: Plastic and Reconstructive Surgery, Ege University Faculty of Medicine, Izmir, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Teymur
authors.affiliation: Histology and Embryology, Ege University Faculty of Medicine, Izmir, Turkey.
authors.forename: U
authors.initials: U
authors.lastname: Ates
authors.affiliation: Histology and Embryology, Ege University Faculty of Medicine, Izmir, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Baka
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D008297:Male; D008787:Metoclopramide / Q000009:adverse effects*; D011899:Ranitidine / Q000009:adverse effects*; D051381:Rats; D017208:Rats, Wistar; D013524:Surgical Flaps; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D011899:Ranitidine; D008787:Metoclopramide