title: Decolorization and biodegradation of congo red dye by a novel white rot fungus Alternaria alternata CMERI F6.
abstract: A novel white rot fungus Alternaria alternata CMERI F6 decolorized 99.99% of 600 mg/L congo red within 48 h in yeast extract-glucose medium at 25 °C, pH 5 and 150 rpm. Physicochemical parameters like carbon and nitrogen sources, temperature, pH and aeration were optimized to develop faster decolorization process. Dye decolorization rate was maximal (20.21 mg/L h) at 25 °C, pH 5, 150 rpm and 800 mg/L dye, giving 78% final decolorization efficiency. Scanning electron microscopy and X-ray Diffraction analysis revealed that the fungus become amorphous after dye adsorption. HPLC and FTIR analysis of the extracted metabolites suggested that the decolorization occurred through biosorption and biodegradation. Thermogravimetric analysis (TGA), differential thermal analysis (DTA) and acid-alkali and 70% ethanol treatment revealed the efficient dye retention capability of the fungus. The foregoing results justify the applicability of the strain in removal of congo red from textile wastewaters and their safe disposal.
authors.affiliation: Department of Biotechnology, National Institute of Technology Durgapur, Mahatma Gandhi Avenue, Durgapur 713209, India.
authors.forename: Samayita
authors.initials: S
authors.lastname: Chakraborty
authors.affiliation: Department of Biotechnology, National Institute of Technology Durgapur, Mahatma Gandhi Avenue, Durgapur 713209, India.
authors.forename: Bikram
authors.initials: B
authors.lastname: Basak
authors.affiliation: Department of Biotechnology, National Institute of Technology Durgapur, Mahatma Gandhi Avenue, Durgapur 713209, India.
authors.forename: Subhasish
authors.initials: S
authors.lastname: Dutta
authors.affiliation: Department of Bio Engineering, National Institute of Technology Agartala, Barjala, Tripura 799055, India. Electronic address: bbhunia@gmail.com.
authors.forename: Biswanath
authors.initials: B
authors.lastname: Bhunia
authors.affiliation: Department of Biotechnology, National Institute of Technology Durgapur, Mahatma Gandhi Avenue, Durgapur 713209, India. Electronic address: apurbadey.bt@gmail.com.
authors.forename: Apurba
authors.initials: A
authors.lastname: Dey
mesh_terms: D000528:Alternaria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D003116:Color; D003224:Congo Red / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D003224:Congo Red
keywords: Alternaria alternata; Azo dyes; Biodegradation; Congo red; Decolorization