title: Association between PRO12ALA polymorphism of the PPAR-γ2 gene and type 2 diabetes mellitus in Iranian patients.
abstract: BACKGROUND
  Peroxisome proliferator-activated receptor (PPARs) have been identified as ligand-activated transcription factors that belong to the nuclear receptor superfamily. It has been shown that an association exists between Proline 12 alanine (Pro12Ala) polymorphism of PPAR-GAMMA2 (PPAR-γ2) gene and increased risk of type 2 diabetes mellitus (T2DM) in different populations. Therefore, the present study was designed to investigate the association between Pro12Ala polymorphism of PPAR-γ2 gene and T2DM in an Iranian population.
  
  
  METHODS
  Two hundred unrelated people, including 100 healthy controls and 100 diabetic patients were recruited diagnosed based on American Diabetes Association criteria. Blood samples were used for isolation of genomic deoxyribonucleic acid (DNA). Having extracted the genomic DNA from human blood leukocytes by means of High Pure polymerase chain reaction (PCR) Template preparation kit, we carried out polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) on each blood sample. Then, Genomic DNA was digested by BstU-I restriction enzyme. Thereafter, restriction products were analyzed by means of Polyacrylamide gel electrophoresis and stained by Ethidium Bromide.
  
  
  RESULTS
  We found that the frequency of Ala allele in healthy subjects was significantly higher than in diabetic subjects (P = 0003). Moreover, the genotype frequency of Ala/Ala in healthy subjects was significantly higher than in diabetic subjects (P < 0.001). However, the genotype frequency of Ala/Pro in diabetic subjects was significantly higher than in healthy subjects (P < 0.001).
  
  
  CONCLUSIONS
  The present study suggests that polymorphism of PPAR-γ2 gene is associated with T2DM. Furthermore, Ala allele is significantly found in non-diabetic individual's Iranian population.
authors.affiliation: Department of Pharmacology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
authors.forename: Azadeh
authors.initials: A
authors.lastname: Motavallian
authors.forename: Sasan
authors.initials: S
authors.lastname: Andalib
authors.forename: Golnaz
authors.initials: G
authors.lastname: Vaseghi
authors.forename: Hamid
authors.initials: H
authors.lastname: Mirmohammad-Sadeghi
authors.forename: Masoud
authors.initials: M
authors.lastname: Amini
keywords: Polymorphism; peroxisome proliferator-activated receptor-GAMMA 2 gene; type 2 diabetes mellitus
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