title: Mycobacterium avium subsp. Paratuberculosis and the expression of selected virulence and pathogenesis genes in response to 6°C, 65°c and ph 2.0.
abstract: The aim of this work was to study the expression of selected Mycobacterium avium subsp. paratuberculosis (MAP) genes connected with MAP virulence, adhesion and stress response. The temperature of 6°C and 65°C were chosen with regard to the food industry, storage conditions (refrigerator) and low-temperature pasteurization. A pH of 2.0, using lactic acid, was selected to mimic the natural environment of the stomach. Expression of selected genes was studied using real time reverse transcription PCR on three different MAP isolates. MAP isolates were chosen according to the number of their preceding cultivations. While isolates 8672 and 8819 were previously cultivated only once, MAP isolate 12146 went through four passages. Different expression profiles were observed in each of the three MAP isolates. However, particular similar patterns were observed. SigE, sigF and ahpC were up-regulated, while sigL was down-regulated under temperature stress. Mmp gene was found to be down-regulated under acidic conditions. Low passage isolates (8672 and 8819) showed certain level of acid resistance.
authors.affiliation: Veterinary Research Institute , Hudcova 70, 621 00 Brno , Czech Republic;
authors.forename: Radka
authors.initials: R
authors.lastname: Pribylova
authors.forename: Petr
authors.initials: P
authors.lastname: Kralik
authors.forename: Neysan
authors.initials: N
authors.lastname: Donnelly
authors.forename: Jan
authors.initials: J
authors.lastname: Matiasovic
authors.forename: Ivo
authors.initials: I
authors.lastname: Pavlik
keywords: Johne’s disease; MAP; real time PCR; stress; treatment
references.citation: Methods Mol Biol. 2000;132:365-86
references.pmid: 10547847
references.citation: Mol Microbiol. 1999 Jan;31(2):715-24
references.pmid: 10027986
references.citation: J Appl Microbiol. 2005;98(6):1282-93
references.pmid: 15916642
references.citation: Trends Microbiol. 2005 Nov;13(11):505-9
references.pmid: 16140533
references.citation: Infect Immun. 2000 Feb;68(2):801-8
references.pmid: 10639449
references.citation: J Wildl Dis. 2006 Oct;42(4):724-31
references.pmid: 17255438
references.citation: Science. 1995 Oct 20;270(5235):484-7
references.pmid: 7570003
references.citation: Cell. 1977 Nov;12(3):721-32
references.pmid: 922889
references.citation: Am J Gastroenterol. 2005 Jul;100(7):1529-36
references.pmid: 15984976
references.citation: Lett Appl Microbiol. 1999 Jun;28(6):461-5
references.pmid: 10389265
references.citation: Genome Biol. 2002 Oct 24;3(11):research0062
references.pmid: 12429061
references.citation: Infect Immun. 2006 Jul;74(7):3825-33
references.pmid: 16790754
references.citation: FEMS Immunol Med Microbiol. 2005 Feb 1;43(2):185-95
references.pmid: 15681149
references.citation: J Bacteriol. 2005 Apr;187(7):2406-15
references.pmid: 15774884
references.citation: J Microbiol Methods. 2004 May;57(2):241-9
references.pmid: 15063064
references.citation: Am J Epidemiol. 2007 Apr 1;165(7):776-83
references.pmid: 17237136
references.citation: Genome Biol. 2002 Jun 18;3(7):RESEARCH0034
references.pmid: 12184808
references.citation: Infect Immun. 2003 Oct;71(10):6083-7
references.pmid: 14500535
references.citation: J Microbiol Methods. 2006 Nov;67(2):310-20
references.pmid: 16753236
references.citation: Int J Food Microbiol. 2008 Dec 10;128(2):250-7
references.pmid: 18824269
references.citation: Proc Natl Acad Sci U S A. 2005 Aug 30;102(35):12344-9
references.pmid: 16116077
references.citation: Vet Microbiol. 2001 Mar 20;79(2):123-31
references.pmid: 11230934
references.citation: FEMS Microbiol Lett. 2001 Mar 15;196(2):195-9
references.pmid: 11267779
references.citation: J Bacteriol. 1993 Jul;175(13):4255-9
references.pmid: 8320241
references.citation: Rev Sci Tech. 2001 Apr;20(1):133-50
references.pmid: 11288509
references.citation: Vet Pathol. 2004 Jul;41(4):326-37
references.pmid: 15232132
references.citation: Infect Immun. 2006 Apr;74(4):2457-61
references.pmid: 16552079
references.citation: Curr Microbiol. 2008 Jun;56(6):574-80
references.pmid: 18324436
references.citation: Infect Genet Evol. 2007 Jul;7(4):424-32
references.pmid: 17292677
references.citation: Infect Immun. 1994 Feb;62(2):476-83
references.pmid: 7507894
references.citation: Lancet. 2004 Sep 18-24;364(9439):1039-44
references.pmid: 15380962
references.citation: Appl Environ Microbiol. 1998 Mar;64(3):999-1005
references.pmid: 9501439
references.citation: Appl Environ Microbiol. 2002 May;68(5):2428-35
references.pmid: 11976118
references.citation: Nucleic Acids Res. 2001 May 1;29(9):e45
references.pmid: 11328886
references.citation: J Bacteriol. 2005 Oct;187(20):7062-71
references.pmid: 16199577
references.citation: Inflamm Bowel Dis. 2006 Jan;12(1):62-9
references.pmid: 16374261
references.citation: J Bacteriol. 2007 Nov;189(21):7877-86
references.pmid: 17693514
references.citation: Infect Immun. 1997 Nov;65(11):4548-57
references.pmid: 9353032
references.citation: Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):2790-4
references.pmid: 8610119
references.citation: Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4
references.pmid: 414220
references.citation: J Clin Microbiol. 2003 Jul;41(7):2915-23
references.pmid: 12843021
references.citation: Nat Genet. 1992 Nov;2(3):173-9
references.pmid: 1345164
references.citation: Microbiology (Reading). 2003 Aug;149(Pt 8):2061-2069
references.pmid: 12904546
references.citation: Curr Opin Drug Discov Devel. 2003 May;6(3):384-95
references.pmid: 12833672
references.citation: J Bacteriol. 1992 Jan;174(1):1-7
references.pmid: 1729202