title: The human kinesin-14 HSET tracks the tips of growing microtubules in vitro.
abstract: Tip-tracking of kinesin-14 motor proteins is believed to be crucial for the assembly and maintenance of dynamic microtubule arrays. However, in contrast to other members of the kinesin-14 family, H. sapiens kinesin-14 HSET has so far never been observed to be prominently located at microtubule plus ends. Here, using an in vitro microtubule dynamics reconstitution assay we observe tip-tracking of GFP-HSET in the presence of H. sapiens EB1 (hsEB1). Tip-tracking depended on the SxIP-like motif in HSET as well as on the EB homology domain in hsEB1. D. melanogaster Ncd and S. pombe Klp2 tip-tracking reconstitution assays accompanied by kinesin-14 amino acid sequence comparisons suggest that SxIP-like motif mediated tip-tracking dependent on EB family proteins is conserved in the kinesin-14 family of molecular motors.
authors.affiliation: B CUBE - Center for Molecular Bioengineering, Technische Universität Dresden, Arnoldstrasse 18, Dresden, 01307, Germany; Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, Dresden, 01307, Germany.
authors.forename: Marcus
authors.initials: M
authors.lastname: Braun
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Lansky
authors.forename: Seweryn
authors.initials: S
authors.lastname: Bajer
authors.forename: Gero
authors.initials: G
authors.lastname: Fink
authors.forename: Andrzej A
authors.initials: AA
authors.lastname: Kasprzak
authors.forename: Stefan
authors.initials: S
authors.lastname: Diez
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004331:Drosophila melanogaster; D006801:Humans; D016547:Kinesins / Q000737:chemistry / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data
chemical_list: C486566:KIFC1 protein, human; D008869:Microtubule-Associated Proteins; D016547:Kinesins
keywords: EB1; HSET; kinesin-14; microtubule; tip-tracking