title: CD31 expression on peripheral blood stem cells predicts both early neutrophil and platelet engraftments.
abstract: No detailed information currently exists about the immune phenotypic profiles of peripheral blood stem cells (PBSCs) obtained by different mobilization regimens. The effects of these profiles on the outcome of transplantation are largely unknown. In this prospective study, the surface immune phenotypic features (CD11a, CD18, CD31, CD38, CD44, CD62e, CD62L, CD90, CD117, CD135 and CD184 expression) of sorted PBSCs that had been mobilized by growth factor with (group I and group II) or without (group III) disease-specific chemotherapies were investigated. The immune phenotypic features on mobilized PBSCs in groups I, II and III were not significantly different. The CD31 (platelet endothelial cell adhesion molecule-1) positivity ratio on PBSCs inversely correlated with both the duration of neutrophil (r=-0.32, p=0.03) and platelet (r=-0.36, p=0.02) engraftment. No relationship was found between the engraftment (neutrophil and platelet) durations and CD184 (chemokine receptor CXC motif receptor 4 [CXCR4]) expression on PBSCs. We demonstrated that the surface immune phenotypic profiles on PBSCs obtained by several mobilization regimens were not different. To our knowledge, this is the first study to demonstrate that CD31 expression on human PBSCs may positively affect both neutrophil and platelet engraftment. Contrary to our expectations, CD184 (CXCR4) expression on PBSCs has no effect on neutrophil or platelet engraftment. Considered together, our results suggest that additional surface antigens (such as CD31) may be more effective in the homing process.
authors.affiliation: Ege University Medical School, Department of Hematology, 35100 Izmir, Turkey. Electronic address: ayhan.donmez@ege.edu.tr.
authors.forename: Ayhan
authors.initials: A
authors.lastname: Donmez
authors.forename: Murat
authors.initials: M
authors.lastname: Tombuloglu
authors.forename: Okan
authors.initials: O
authors.lastname: Gulbahar
authors.forename: Bahar
authors.initials: B
authors.lastname: Arik
authors.forename: Seckin
authors.initials: S
authors.lastname: Cagirgan
authors.forename: Filiz
authors.initials: F
authors.lastname: Vural
authors.forename: Nihal
authors.initials: N
authors.lastname: Gokmen
mesh_terms: D000328:Adult; D000368:Aged; D001792:Blood Platelets / Q000378:metabolism / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006085:Graft Survival; D019337:Hematologic Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism / Q000473:pathology; D036102:Peripheral Blood Stem Cell Transplantation; D011237:Predictive Value of Tests; D011446:Prospective Studies
keywords: CD31; CXCR4; Homing; Mobilization; Peripheral blood stem cells