title: Proteomic identification of carbonylated proteins in the kidney of trichloroethene-exposed MRL+/+ mice.
abstract: Trichloroethene (TCE), a common environmental and occupational pollutant, is associated with multiorgan toxicity. Kidney is one of major target organs affected as a result of TCE exposure. Our previous studies have shown that exposure to TCE causes increased protein oxidation (protein carbonylation) in the kidneys of autoimmune-prone MRL+/+ mice, and suggested a potential role of protein oxidation in TCE-mediated nephrotoxicity. To assess the impact of chronic TCE exposure on protein oxidation, particularly to identify the carbonylated proteins in kidneys, female MRL+/+ mice were treated with TCE at the dose of 2 mg/ml via drinking water for 36 weeks and kidney protein extracts were analyzed for protein carbonyls and carbonylated proteins identified using proteomic approaches (2D gel, Western blot, MALDI TOF/TOF MS/MS, etc.). TCE treatment led to significantly increased protein carbonyls in the kidney protein extracts (20 000 g pellet fraction). Interestingly, among 18 identified carbonylated proteins, 10 were found only in the kidneys of TCE-treated mice, whereas other 8 were present in the kidneys of both control and TCE-treated mice. The identified carbonylated proteins represent skeletal proteins, chaperones, stress proteins, enzymes, plasma protein and proteins involved in signaling pathways. The findings provide a map for further exploring the role of carbonylated proteins in TCE-mediated nephrotoxicity.
authors.affiliation: Department of Pathology and.
authors.forename: Xiuzhen
authors.initials: X
authors.lastname: Fan
authors.forename: Gangduo
authors.initials: G
authors.lastname: Wang
authors.forename: Robert D
authors.initials: RD
authors.lastname: English
authors.forename: M
authors.initials: M
authors.lastname: Firoze Khan
mesh_terms: D000818:Animals; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D015151:Immunoblotting; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D019463:Mice, Inbred MRL lpr; D008822:Mice, Transgenic; D010084:Oxidation-Reduction; D050050:Protein Carbonylation / Q000187:drug effects*; D014241:Trichloroethylene / Q000633:toxicity*
chemical_list: D014241:Trichloroethylene
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