title: Hollow graphitic nanocapsules as efficient electrode materials for sensitive hydrogen peroxide detection.
abstract: Carbon nanomaterials are typically used in electrochemical biosensing applications for their unique properties. We report a hollow graphitic nanocapsule (HGN) utilized as an efficient electrode material for sensitive hydrogen peroxide detection. Methylene blue (MB) molecules could be efficiently adsorbed on the HGN surfaces, and this adsorption capability remained very stable under different pH regimes. HGNs were used as three-dimensional matrices for coimmobilization of MB electron mediators and horseradish peroxidase (HRP) to build an HGN-HRP-MB reagentless amperometric sensing platform to detect hydrogen peroxide. This simple HGN-HRP-MB complex demonstrated very sensitive and selective hydrogen peroxide detection capability, as well as high reproducibility and stability. The HGNs could also be utilized as matrices for immobilization of other enzymes, proteins or small molecules and for different biomedical applications.
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha 410082, PR China.
authors.forename: Wei-Na
authors.initials: WN
authors.lastname: Liu
authors.forename: Ding
authors.initials: D
authors.lastname: Ding
authors.forename: Zhi-Ling
authors.initials: ZL
authors.lastname: Song
authors.forename: Xia
authors.initials: X
authors.lastname: Bian
authors.forename: Xiang-Kun
authors.initials: XK
authors.lastname: Nie
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Zhang
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Chen
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000379:methods*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000302:isolation & purification*; D008751:Methylene Blue; D053769:Nanocapsules / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D053769:Nanocapsules; D037742:Nanotubes, Carbon; D011506:Proteins; D006108:Graphite; D006861:Hydrogen Peroxide; D008751:Methylene Blue
keywords: Amperometric sensors; Electrode materials; Hollow graphitic nanocapsule; Hydrogen peroxide; Methylene blue