title: Investigation of potential markers of acid resistance in Lactobacillus plantarum by comparative proteomics.
abstract: OBJECTIVE
  To investigate cell determinants of resistance to gastric acidity in Lactobacillus plantarum using comparative proteomics.
  
  
  RESULTS
  Among ten Lact. plantarum strains that were tested for their acid resistance in vitro, three strains with different phenotypes were selected for comparative proteomic analysis: LC 804 (resistant), CIP A159 (intermediate) and CECT 4185 (sensitive). Constitutive differences between whole-cell proteomes of the three strains were studied. Among the differentially expressed proteins between strains, 17 have previously been reported to be involved in acid resistance processes. The effect of a low-pH exposure on these proteomic patterns was investigated, which led to the identification of five putative determinants of acid resistance (heat-shock protein GrpE, methionine synthase and 30S ribosomal protein S2) or sensitivity (phosphotransacetylase and adenylosuccinate synthase). Analysis also revealed three additional proteins involved in cell envelope biogenesis (3-oxoacyl-synthase II, dTDP-glucose 4,6-dehydratase and dTDP-4-dehydrorhamnose 3,5-epimerase) likely to be key factors of intrinsic acid tolerance in Lact. plantarum.
  
  
  CONCLUSIONS
  The approach used in this study enabled the identification of potential markers of acid tolerance in Lact. plantarum, which may serve for phenotyping and screening purposes.
  
  
  CONCLUSIONS
  The present work represents a further step towards the identification of bacterial biomarkers for each particular probiotic feature.
authors.affiliation: Equipe de Chimie Analytique des Molécules Bio-Actives, IPHC-DSA, Université de Strasbourg, CNRS, Illkirch-Graffenstaden, France; Aérial Parc d'Innovation, Illkirch-Graffenstaden, France.
authors.forename: E
authors.initials: E
authors.lastname: Hamon
authors.forename: P
authors.initials: P
authors.lastname: Horvatovich
authors.forename: E
authors.initials: E
authors.lastname: Marchioni
authors.forename: D
authors.initials: D
authors.lastname: Aoudé-Werner
authors.forename: S
authors.initials: S
authors.lastname: Ennahar
keywords: Lactobacillus plantarum; acid resistance; biomarker; comparative proteomics; probiotics