title: Guided bone regeneration using acellular bovine pericardium in a rabbit mandibular model: in-vitro and in-vivo studies.
abstract: OBJECTIVE
  To investigate the feasibility of acellular bovine pericardium (BP) for guided bone regeneration (GBR) in vitro and in vivo. The success of GBR relies on the fact that various cellular components possess different migration rates into the defect site and that a barrier membrane plays a significant role in these processes.
  
  
  METHODS
  BP membrane was isolated and decellularized using an enzymatic method. The microarchitecture, mechanical properties, cytotoxicity and cell chemotaxis properties of the acellular BP were evaluated in vitro, and the in-vivo efficacy of the acellular BP was also investigated in a rabbit mandibular model.
  
  
  RESULTS
  The acellular BP membrane possessed an interconnected fibrous structure. Glutaraldehyde (GA) treatment was efficient for enhancement of the mechanical properties of the acellular BP bur and resulted in negligible cytotoxicity. After 16 wk, standardized osseous defects created in the rabbit mandible, and covered with acellular BP, were associated with an enhanced deposition of mineralized tissue when compared with defects left to spontaneous healing.
  
  
  CONCLUSIONS
  GA-treated acellular BP is promising as a barrier membrane for GBR for further in-vivo and clinical studies.
authors.affiliation: Department of Stomatology, Second Xiangya Hospital, Central South University, Changsha, 410011, Hunan, China.
authors.forename: M
authors.initials: M
authors.lastname: Bai
authors.forename: T
authors.initials: T
authors.lastname: Zhang
authors.forename: T
authors.initials: T
authors.lastname: Ling
authors.forename: Z
authors.initials: Z
authors.lastname: Zhou
authors.forename: H
authors.initials: H
authors.lastname: Xie
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: G
authors.initials: G
authors.lastname: Hu
authors.forename: C
authors.initials: C
authors.lastname: Jiang
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: B
authors.initials: B
authors.lastname: Feng
authors.forename: H
authors.initials: H
authors.lastname: Wu
mesh_terms: D016475:3T3 Cells; D015502:Absorptiometry, Photon; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001705:Bioprosthesis; D015519:Bone Density / Q000502:physiology; D002113:Calcification, Physiologic / Q000502:physiology; D002417:Cattle; D002633:Chemotaxis / Q000502:physiology; D004195:Disease Models, Animal; D005240:Feasibility Studies; D048091:Guided Tissue Regeneration / Q000379:methods*; D008336:Mandibular Diseases / Q000601:surgery; D008567:Membranes, Artificial; D051379:Mice; D010496:Pericardium / Q000737:chemistry / Q000648:ultrastructure; D011817:Rabbits; D013314:Stress, Mechanical; D013499:Surface Properties; D013696:Temperature; D013718:Tensile Strength; D014021:Tissue Preservation / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial
keywords: acellular bovine pericardium; cytotoxicity; guided bone regeneration; rabbit mandibular model