title: Beneficial effects of wheat gluten hydrolysate to extend lifespan and induce stress resistance in nematode Caenorhabditis elegans.
abstract: Previous studies have showed that wheat gluten hydrolysate (WGH) has the anti-oxidative property. In the present study, we examined the possible safety property of WGH and the beneficial effects of WGH to extend lifespan and induce stress resistance using nematode Caenorhabditis elegans as the in vivo assay system. We found that WGH at concentrations of 0.1-1 mg/mL did not cause lethality, influence development, alter locomotion behavior and brood size, and induce significant intestinal autofluorescence and reactive oxygen species (ROS) production in young adults. Treatment with 0.1-1 mg/mL of WGH significantly extended lifespans of nematodes under the normal conditions. Moreover, WGH treatment significantly inhibited the induction of intestinal autofluorescence and suppressed the decrease in locomotion behavior during the aging process of nematodes. Furthermore, pre-treatment with 1 mg/mL of WGH significantly suppressed the adverse effects caused by heat-stress or oxidative stress on nematodes as indicated by the alterations of both lifespan and intestinal ROS production. Therefore, WGH treatment is relatively safe and has beneficial effects on nematodes under both the normal conditions and the stress conditions.
authors.affiliation: College of Life Sciences, Nanjing Normal University, Nanjing, China ; Nanjing Institute for Comprehensive Utilization of Wild Plants, Nanjing, China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Zhang
authors.forename: Ting
authors.initials: T
authors.lastname: Lv
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Qiuli
authors.initials: Q
authors.lastname: Wu
authors.forename: Linsong
authors.initials: L
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Dafeng
authors.initials: D
authors.lastname: Sun
authors.forename: Lingmei
authors.initials: L
authors.lastname: Sun
authors.forename: Ziheng
authors.initials: Z
authors.lastname: Zhuang
authors.forename: Dayong
authors.initials: D
authors.lastname: Wang
mesh_terms: D000375:Aging / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000502:physiology; D052284:Clutch Size / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005983:Glutens / Q000737:chemistry / Q000494:pharmacology*; D006358:Hot Temperature; D006868:Hydrolysis; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects; D008124:Locomotion / Q000187:drug effects / Q000502:physiology; D008136:Longevity / Q000187:drug effects* / Q000502:physiology; D061848:Optical Imaging; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D005983:Glutens
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