title: Taxonomic study and partial characterization of antimicrobial compounds from a moderately halophilic strain of the genus Actinoalloteichus.
abstract: A moderately halophilic actinomycete strain designated AH97 was isolated from a saline Saharan soil, and selected for its antimicrobial activities against bacteria and fungi. The AH97 strain was identified by morphological, chemotaxonomic and phylogenetic analyses to the genus Actinoalloteichus. Analysis of the 16S rDNA sequence of strain AH97 showed a similarity level ranging between 95.8% and 98.4% within Actinoalloteichus species, with A. hymeniacidonis the most closely related. The comparison of the physiological characteristics of AH97 with those of known species of Actinoalloteichus showed significant differences. Strain AH97 showed an antibacterial and antifungal activity against broad spectrum of microorganisms known to be human and plant pathogens. The bioactive compounds were extracted from the filtrate culture with n-butanol and purified using thin layer chromatography and high pressure liquid chromatography procedures. Two active products were isolated, one hydrophilic fraction (F1) and another hydrophobic (F2). Ultraviolet-visible, infrared, mass and (1)H and (13)C nuclear magnetic resonance spectroscopy studies suggested that these molecules were the dioctyl phthalate (F2) and an aminoglycosidic compound (F1).
authors.affiliation: Laboratoire de Recherche sur les Produits Bioactifs et la Valorisation de la Biomasse, Ecole Normale Supérieure de Kouba , BP 92, 16050 Kouba, Alger , Algérie.
authors.forename: Farida
authors.initials: F
authors.lastname: Boudjelal
authors.forename: Abdelghani
authors.initials: A
authors.lastname: Zitouni
authors.forename: Florence
authors.initials: F
authors.lastname: Mathieu
authors.forename: Ahmed
authors.initials: A
authors.lastname: Lebrihi
authors.forename: Nasserdine
authors.initials: N
authors.lastname: Sabaou
keywords: Actinoalloteichus; biological activities; dioctyl phthalate; structure; taxonomy
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