title: Nanohybrids of silver particles on clay platelets delaminate Pseudomonas biofilms.
abstract: OBJECTIVE
  To evaluate the effectiveness of novel nanohybrids, composed of silver nanoparticles and nanoscale silicate platelets, to clear Pseudomonas aeruginosa biofilms.
  
  
  METHODS
  The nanohybrids were manufactured from an in situ reduction of silver salts in the silicate platelet dispersion, and then applied to biofilms in vitro and in vivo.
  
  
  RESULTS
  In reference to the biocidal effects of gentamycin, the nanohybrids mitigated the spreading of the biofilms, and initiated robust cell death and exfoliation from the superficial layers of the biofilms in vitro. In vivo, the nanohybrids exhibited significant therapeutic effects by eliminating established biofilms from infected corneas and promoting the recovery of corneal integrity.
  
  
  CONCLUSIONS
  All of the evaluations indicate the high potency of the newly developed silver nanoparticle/nanoscale silicate platelet nanohybrids for eliminating biofilms.
authors.affiliation: Department of Life Sciences, National Chung Hsing University, Taiwan.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Tseng
authors.forename: Chih-Yin
authors.initials: CY
authors.lastname: Juan
authors.forename: Shiun-Long
authors.initials: SL
authors.lastname: Lin
authors.forename: Michael R
authors.initials: MR
authors.lastname: Doran
authors.forename: Jiang-Jen
authors.initials: JJ
authors.lastname: Lin
authors.forename: Shan-Hui
authors.initials: SH
authors.lastname: Hsu
authors.forename: Jiunn-Wang
authors.initials: JW
authors.lastname: Liao
authors.forename: Ching-I
authors.initials: CI
authors.lastname: Shen
authors.forename: Hong-Lin
authors.initials: HL
authors.lastname: Su
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry* / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D000077215:Clay; D005839:Gentamicins / Q000737:chemistry / Q000494:pharmacology; D053768:Metal Nanoparticles / Q000737:chemistry*; D011549:Pseudomonas / Q000187:drug effects*; D012834:Silver / Q000737:chemistry*
chemical_list: D000538:Aluminum Silicates; D005839:Gentamicins; D012834:Silver; D000077215:Clay
keywords: biofilm; clay; infection; keratitis; silver nanoparticle