title: Adsorption properties and preparative separation of phenylethanoid glycosides from Cistanche deserticola by use of macroporous resins.
abstract: A simple and efficient chromatographic method for large-scale preparative separation of phenylethanoid glycosides (mainly contain echinacoside and acteoside) from Cistanche deserticola was developed. The adsorption properties of eight macroporous resins were evaluated. Three selected resins were further screened depending on the adsorption kinetics curves, in which HPD300 resin showed the best separation efficiency. The adsorption isotherm data on HPD300 resin were fitted to the Freundlich equation in certain concentration range. The dynamic adsorption and desorption experiments were carried out on columns packed with HPD300 resin to optimize the separation process. The breakthrough curves showed that acteoside had a higher affinity to the resin than echinacoside. The contents of echinacoside and acteoside in the product increased from 1.79% and 1.43% in the crude extracts to 16.66% and 15.17%, with recovery yields of 80.41% and 90.17%, respectively. The purity of total phenylethanoid glycosides in the product was 76.58%.
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190 Beijing, PR China; Department of Food Science and Engineering, College of Biological Science and Biotechnology, Beijing Forestry University, 100083 Beijing, PR China.
authors.forename: Boyan
authors.initials: B
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Ouyang
authors.forename: Xiaofan
authors.initials: X
authors.lastname: Yuan
authors.forename: Liwei
authors.initials: L
authors.lastname: Wang
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D031671:Cistanche / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D006027:Glycosides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D007700:Kinetics; D010636:Phenols / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*; D013696:Temperature; D059808:Polyphenols
chemical_list: D005960:Glucosides; D006027:Glycosides; D010636:Phenols; D010936:Plant Extracts; C058956:acteoside; D059808:Polyphenols; C060297:echinacoside
keywords: Acteoside; Cistanche deserticola Y. C. Ma; Echinacoside; Macroporous resin; Phenylethanoid glycosides; Separation