title: Isolation of leptospira Serovars Canicola and Copenhageni from cattle urine in the state of ParanÁ, Brazil.
abstract: In 2001, 698 urine samples were randomly collected from cattle at a slaughterhouse in the State of Paraná, Brazil. Direct examination using dark field microscopy was carried out immediately after collection. Five putative positive samples were cultured in modified EMJH medium, yielding two positive cultures (LO-14 and LO-10). Typing with monoclonal antibodies revealed that the two isolates were similar to Canicola (LO-14) and Copenhageni (LO-10). Microscopic agglutination test results show that Hardjo is the most common serovar in cattle in Brazil. Rats and dogs are the common maintenance hosts of serovars Copenhageni and Canicola. The excretion of highly pathogenic serovars such as Copenhageni and Canicola by cattle can represent an increasing risk for severe leptospirosis is large populations, mainly living in rural areas.
authors.affiliation: Programa de Pós-graduação em Ciência Animal, Laboratório de Leptospirose Animal, Universidade Estadual de Londrina , Londrina, PR , Brasil.
authors.forename: Francielle Gibson da Silva
authors.initials: FG
authors.lastname: Zacarias
authors.forename: Silvio Arruda
authors.initials: SA
authors.lastname: Vasconcellos
authors.forename: Eleine Kuroki
authors.initials: EK
authors.lastname: Anzai
authors.forename: Nilson
authors.initials: N
authors.lastname: Giraldi
authors.forename: Julio Cesar
authors.initials: JC
authors.lastname: de Freitas
authors.forename: Rudy
authors.initials: R
authors.lastname: Hartskeerl
keywords: Leptospira; cattle; culture; diagnosis; leptospirosis
references.citation: Am J Vet Res. 1986 Aug;47(8):1694-6
references.pmid: 3752677
references.citation: Vet Rec. 1996 Mar 23;138(12):272-6
references.pmid: 8711883
references.citation: Lancet. 1999 Sep 4;354(9181):820-5
references.pmid: 10485724
references.citation: Rev Inst Med Trop Sao Paulo. 1992 May-Jun;34(3):217-21
references.pmid: 1342073
references.citation: Vet Rec. 1982 Feb 13;110(7):147-50
references.pmid: 7039082
references.citation: Ann Clin Microbiol Antimicrob. 2006 Nov 23;5:28
references.pmid: 17121682
references.citation: Zentralbl Bakteriol A. 1981 Aug;249(3):400-6
references.pmid: 7269858
references.citation: Vet Rec. 1992 Nov 7;131(19):435-9
references.pmid: 1455593
references.citation: Onderstepoort J Vet Res. 1982 Mar;49(1):57-62
references.pmid: 7122066
references.citation: Res Vet Sci. 1985 Nov;39(3):292-5
references.pmid: 2417297
references.citation: Vet Microbiol. 1986 Nov;12(4):377-81
references.pmid: 3788051
references.citation: Curr Opin Infect Dis. 2005 Oct;18(5):376-86
references.pmid: 16148523
references.citation: Vet Rec. 1981 Jun 27;108(26):555-7
references.pmid: 7303438
references.citation: Aust Vet J. 1993 Oct;70(10):393-4
references.pmid: 8257323
references.citation: Vet Clin North Am Food Anim Pract. 1994 Nov;10(3):463-78
references.pmid: 7728630
references.citation: Int J Syst Bacteriol. 1999 Apr;49 Pt 2:839-58
references.pmid: 10319510
references.citation: Vet Rec. 1992 May 16;130(20):446-8
references.pmid: 1621343
references.citation: PLoS Med. 2006 Aug;3(8):e308
references.pmid: 16933963