title: Genetic similarity between staphylococcus sp isolated from human and hospital settings, and susceptibility to different antimicrobials.
abstract: One hundred and forty-three samples from human hands and hospital beds were collected at a teaching hospital in the city of Ribeirão Preto/SP by swabs, and placed in BHI broth. Following a 24 h incubation period at 37°C, they were seeded on Petri dishes containing Agar "Staphylococcus Medium 110". Colonies typical of the genus Staphylococcus were collected and stored at 4°C until tested for catalase, mannitol, hemolysis, DNAse and coagulase. Strains were analyzed by RAPD-PCR to verify their similarity, and tested for sensitivity to ten different antibiotics. From the ninety-two isolated strains, 67 (72,8%) were coagulase- negative and 25 (27,2%) coagulase-positive. Similarity analysis showed a great heterogeneity among strains, but some presented 100% similarity. Resistance to oxacilin was encountered in 39 (42%) of the strains. Two coagulase-negative strains were resistant to vancomycin, and eleven (12%) were considered multiresistant. Measures such as hand disinfection of the staff and hospital beds and rationalization of antibiotic use could contribute to decrease pathogen transmission and selection pressure, diminishing the frequency and lethality of nosocomial infections.
authors.affiliation: Programa de Pós-Graduação em Microbiologia Agropecuária, Universidade Estadual Paulista , Jaboticabal, SP , Brasil.
authors.forename: Telma Luciana Trovó
authors.initials: TL
authors.lastname: Fabiano
authors.forename: Bruna Helena Pinheiro
authors.initials: BH
authors.lastname: de Ávila
authors.forename: Cleusa Cascaes
authors.initials: CC
authors.lastname: Dias
authors.forename: Renato Pariz
authors.initials: RP
authors.lastname: Maluta
authors.forename: Fernando Antonio
authors.initials: FA
authors.lastname: de Ávila
keywords: RAPD-PCR; Similarity; Staphylococcus; multiresistance
references.citation: J Hosp Infect. 2004 May;57(1):31-7
references.pmid: 15142713
references.citation: Clin Microbiol Rev. 2006 Jul;19(3):512-30
references.pmid: 16847083
references.citation: Crit Care Med. 1997 Nov;25(11):1862-7
references.pmid: 9366771
references.citation: J Hosp Infect. 2004 Jan;56(1):56-63
references.pmid: 14706272
references.citation: Clin Infect Dis. 1997 Sep;25(3):584-99
references.pmid: 9314444
references.citation: N Engl J Med. 1998 Aug 20;339(8):520-32
references.pmid: 9709046
references.citation: Microbiol Immunol. 2007;51(9):787-95
references.pmid: 17895595
references.citation: Infect Control Hosp Epidemiol. 2006 Oct;27(10):1128-30
references.pmid: 17006822
references.citation: J Hosp Infect. 2003 Apr;53(4):297-303
references.pmid: 12660127
references.citation: Diagn Microbiol Infect Dis. 1999 Jul;34(3):185-91
references.pmid: 10403098
references.citation: J Clin Microbiol. 2000 Aug;38(8):2985-8
references.pmid: 10921964
references.citation: Int J Med Microbiol. 2002 Jul;292(2):67-74
references.pmid: 12195737
references.citation: J Hosp Infect. 2007 Jun;66(2):192-3
references.pmid: 17512637
references.citation: J Clin Microbiol. 2000 Feb;38(2):866-9
references.pmid: 10655401
references.citation: Infect Control Hosp Epidemiol. 2001 Jul;22(7):443-8
references.pmid: 11583214
references.citation: Lancet Infect Dis. 2008 May;8(5):289-301
references.pmid: 18471774
references.citation: J Clin Microbiol. 1998 Jul;36(7):2038-42
references.pmid: 9650958
references.citation: J Hosp Infect. 2008 May;69(1):8-23
references.pmid: 18355943
references.citation: J Infect. 2006 Apr;52(4):282-9
references.pmid: 16045993
references.citation: J Clin Microbiol. 1998 Apr;36(4):1020-7
references.pmid: 9542929
references.citation: Braz J Med Biol Res. 2004 Sep;37(9):1345-51
references.pmid: 15334200
references.citation: Diagn Microbiol Infect Dis. 2000 Apr;36(4):261-8
references.pmid: 10764969