title: [Impact and potential mechanism of human α-defensin 1 on low-density lipoprotein oxidation ability of ECV304 cells].
abstract: OBJECTIVE
  To explore the role and potential mechanism of human α-defensin 1 (HNP-1) on low-density lipoprotein (LDL) oxidation ability of human endothelial cells (EVC304).
  
  
  METHODS
  Post incubation with LDL for 3 h, the malondialdehyde (MDA) and protein carbonyl (PCO) were detected in untreated ECV304 (control) and in HNP-1 transfected ECV304 in the presence and absence of siRNA against HNP-1. Flow cytometry and fluorescence microscopy were used to detect the generation of oxygen free radical in the ECV304 which have been pretreated by LDL, LPS and HNP-1, respectively.
  
  
  RESULTS
  Compared with control group, MDA level was significantly increased in HNP-1 transfected [(4.21 ± 0.03) vs. (3.15 ± 0.02) nmol/mg · pro] or in HNP-1 stimulated ECV304 cells [(14.49 ± 1.10) vs. (9.47 ± 1.18) nmol/mg · pro], which could be significantly downregulated by siRNA [(3.76 ± 0.48) vs. (4.54 ± 0.28) nmol/mg·pro, all P < 0.05]. PCO was also significantly increased in HNP-1 transfected ECV304 cells. The levels of free radical were significantly increased in HNP-1 transfected or HNP-1 stimulated ECV304 cells.
  
  
  CONCLUSIONS
  HNP-1 can enhance the LDL oxidation ability of human endothelial cells via promoting the generation of free radicals.
authors.affiliation: Department of Molecular Biology, Wuhan University, Wuhan, China.
authors.forename: Cheng-jie
authors.initials: CJ
authors.lastname: Xu
authors.forename: Jing-wei
authors.initials: JW
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Song
authors.forename: Jun-zhu
authors.initials: JZ
authors.lastname: Wu
authors.forename: Ting-ting
authors.initials: TT
authors.lastname: Song
mesh_terms: D002460:Cell Line; D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D034741:RNA, Small Interfering; D014162:Transfection; D023084:alpha-Defensins / Q000235:genetics* / Q000378:metabolism
chemical_list: D008077:Lipoproteins, LDL; D034741:RNA, Small Interfering; D023084:alpha-Defensins; C406413:human neutrophil peptide 1; C099252:oxidized low density lipoprotein