title: CXCL12 induces lung cancer cell migration by polarized mtDNA redistribution.
abstract: Instability of mitochondrial DNA (mtDNA) has been associated with the initiation and development of cancer, but the specific role of mtDNA in the invasiveness and migration of cancer cells remains unclear. In this study, we investigated whether the chemokine CXCL12 causes intact mitochondria to redistribute in cancer cells and, in this way, to increase cell invasiveness and migration. A549 lung cancer cells with intact mtDNA (mtDNA+) and ρ(0)A549 cells depleted of mtDNA (mtDNA-) by long-term ethidium bromide incubation were examined for their responses to CXCL12 in a transwell migration assay and for mitochondrial distribution by fluorescence microscopy. Intact A549 cells showed significantly increased migration and increased polar distribution of mitochondria (asymmetry)in response to CXCL12. However, ρ(0)A549 cells showed no changes in mitochondrial distribution in response to CXCL12, and only a few ρ(0)A549 cells migrated across the transwell membrane after CXCL12 treatment. These results demonstrate that, in A549 lung cancer cells, intact mitochondrial DNA is necessary for mitochondrial redistribution and a chemotactic response to CXCL12.
authors.forename: Jietao
authors.initials: J
authors.lastname: Ma
authors.forename: Jiahe
authors.initials: J
authors.lastname: Zheng
authors.forename: Yaoyong
authors.initials: Y
authors.lastname: Li
authors.forename: Shuling
authors.initials: S
authors.lastname: Zhang
authors.forename: Dongmei
authors.initials: D
authors.lastname: Bai
authors.forename: Huawei
authors.initials: H
authors.lastname: Zou
authors.forename: Chengbo
authors.initials: C
authors.lastname: Han
mesh_terms: D002465:Cell Movement / Q000235:genetics*; D054377:Chemokine CXCL12 / Q000502:physiology*; D004272:DNA, Mitochondrial / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000648:ultrastructure; D008928:Mitochondria / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D004272:DNA, Mitochondrial
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