title: Inhibition of HIV-1 replication by balsamin, a ribosome inactivating protein of Momordica balsamina.
abstract: Ribosome-inactivating proteins (RIPs) are endowed with several medicinal properties, including antiviral activity. We demonstrate here that the recently identified type I RIP from Momordica balsamina also possesses antiviral activity, as determined by viral growth curve assays and single-round infection experiments. Importantly, this activity is at play even as doses where the RIP has no cytotoxic effect. In addition, balsamin inhibits HIV-1 replication not only in T cell lines but also in human primary CD4(+) T cells. This antiviral compound exerts its activity at a viral replicative step occurring later than reverse-transcription, most likely on viral protein translation, prior to viral budding and release. Finally, we demonstrate that balsamin antiviral activity is broad since it also impedes influenza virus replication. Altogether our results demonstrate that type I RIP can exert a potent anti-HIV-1 activity which paves the way for new therapeutic avenues for the treatment of viral infections.
authors.affiliation: Department of Microbiology and Molecular Medicine, University of Geneva, Switzerland University of Geneva, Geneva, Switzerland ; Fermentation and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, India.
authors.forename: Inderdeep
authors.initials: I
authors.lastname: Kaur
authors.forename: Munish
authors.initials: M
authors.lastname: Puri
authors.forename: Zahra
authors.initials: Z
authors.lastname: Ahmed
authors.forename: Fabien P
authors.initials: FP
authors.lastname: Blanchet
authors.forename: Bastien
authors.initials: B
authors.lastname: Mangeat
authors.forename: Vincent
authors.initials: V
authors.lastname: Piguet
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D024882:Drug Resistance, Viral / Q000235:genetics; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D054884:Host-Pathogen Interactions / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D019169:Jurkat Cells; D029775:Momordica / Q000378:metabolism*; D009154:Mutation; D010940:Plant Proteins / Q000494:pharmacology*; D054788:Ribosome Inactivating Proteins / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000473:pathology / Q000821:virology; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D010940:Plant Proteins; C576086:balsamin; D054788:Ribosome Inactivating Proteins
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