title: Kinetic intermediates en route to the final serpin-protease complex: studies of complexes of α1-protease inhibitor with trypsin.
abstract: Serpin protein protease inhibitors inactivate their target proteases through a unique mechanism in which a major serpin conformational change, resulting in a 70-Å translocation of the protease from its initial reactive center loop docking site to the opposite pole of the serpin, kinetically traps the acyl-intermediate complex. Although the initial Michaelis and final trapped acyl-intermediate complexes have been well characterized structurally, the intermediate stages involved in this remarkable transformation are not well understood. To better characterize such intermediate steps, we undertook rapid kinetic studies of the FRET and fluorescence perturbation changes of site-specific fluorophore-labeled derivatives of the serpin, α1-protease inhibitor (α1PI), which report the serpin and protease conformational changes involved in transforming the Michaelis complex to the trapped acyl-intermediate complex in reactions with trypsin. Two kinetically resolvable conformational changes were observed in the reactions, ascribable to (i) serpin reactive center loop insertion into sheet A with full protease translocation but incomplete protease distortion followed by, (ii) full conformational distortion and movement of the protease and coupled serpin conformational changes involving the F helix-sheet A interface. Kinetic studies of calcium effects on the labeled α1PI-trypsin reactions demonstrated both inactive and low activity states of the distorted protease in the final complex that were distinct from the intermediate distorted state. These studies provide new insights into the nature of the serpin and protease conformational changes involved in trapping the acyl-intermediate complex in serpin-protease reactions and support a previously proposed role for helix F in the trapping mechanism.
authors.affiliation: From the Center for Molecular Biology of Oral Diseases and Department of Periodontics and.
authors.forename: Ashoka A
authors.initials: AA
authors.lastname: Maddur
authors.forename: Richard
authors.initials: R
authors.lastname: Swanson
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Izaguirre
authors.forename: Peter G W
authors.initials: PG
authors.lastname: Gettins
authors.forename: Steven T
authors.initials: ST
authors.lastname: Olson
mesh_terms: D000818:Animals; D020134:Catalytic Domain; D002417:Cattle; D006801:Humans; D007700:Kinetics; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000378:metabolism; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D014357:Trypsin / Q000737:chemistry* / Q000378:metabolism; D000515:alpha 1-Antitrypsin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D046912:Multiprotein Complexes; D000515:alpha 1-Antitrypsin; D014357:Trypsin
keywords: Calcium; F Helix; Fluorescence Resonance Energy Transfer (FRET); Protease Inhibitor; Serine Protease; Serpin; Trypsin; α1-Protease Inhibitor
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