title: Role of Janus kinase 3 in mucosal differentiation and predisposition to colitis.
abstract: Janus kinase 3 (Jak3) is a nonreceptor tyrosine kinase expressed in both hematopoietic and nonhematopoietic cells. Previously, we characterized the functions of Jak3 in cytoskeletal remodeling, epithelial wound healing, and mucosal homeostasis. However, the role of Jak3 in mucosal differentiation and inflammatory bowel disease was not known. In this report, we characterize the role of Jak3 in mucosal differentiation, basal colonic inflammation, and predisposition toward colitis. Using the Jak3 knock-out (KO) mouse model, we show that Jak3 is expressed in colonic mucosa of mice, and the loss of mucosal expression of Jak3 resulted in reduced expression of differentiation markers for the cells of both enterocytic and secretory lineages. Jak3 KO mice showed reduced expression of colonic villin, carbonic anhydrase, secretory mucin muc2, and increased basal colonic inflammation reflected by increased levels of pro-inflammatory cytokines IL-6 and IL-17A in colon along with increased colonic myeloperoxidase activity. The inflammations in KO mice were associated with shortening of colon length, reduced cecum length, decreased crypt heights, and increased severity toward dextran sulfate sodium-induced colitis. In differentiated human colonic epithelial cells, Jak3 redistributed to basolateral surfaces and interacted with adherens junction (AJ) protein β-catenin. Jak3 expression in these cells was essential for AJ localization of β-catenin and maintenance of epithelial barrier functions. Collectively, these results demonstrate the essential role of Jak3 in the colon where it facilitated mucosal differentiation by promoting the expression of differentiation markers and enhanced colonic barrier functions through AJ localization of β-catenin.
authors.affiliation: From the Department of Pharmaceutical Sciences, College of Pharmacy, Texas A&M University System Health Science Center, Kingsville, Texas 78363 and.
authors.forename: Jayshree
authors.initials: J
authors.lastname: Mishra
authors.forename: Raj K
authors.initials: RK
authors.lastname: Verma
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Alpini
authors.forename: Fanyin
authors.initials: F
authors.lastname: Meng
authors.forename: Narendra
authors.initials: N
authors.lastname: Kumar
mesh_terms: D022005:Adherens Junctions / Q000201:enzymology / Q000473:pathology; D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis / Q000235:genetics; D002432:Cecum / Q000201:enzymology / Q000473:pathology; D002454:Cell Differentiation; D002460:Cell Line; D003092:Colitis / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D003106:Colon / Q000201:enzymology / Q000473:pathology; D004847:Epithelial Cells / Q000201:enzymology / Q000473:pathology; D005786:Gene Expression Regulation / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000473:pathology; D053616:Janus Kinase 3 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: D000943:Antigens, Differentiation; D051176:beta Catenin; C507933:JAK3 protein, human; C507935:Jak3 protein, mouse; D053616:Janus Kinase 3
keywords: Colitis; Differentiation; Inflammatory Bowel Disease; Innate Immunity; Jak Kinase; β-Catenin
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