title: Low-potential amperometric determination of purine derivatives through surface oxide regeneration method.
abstract: This article described a novel amperometry which can be used for determination of purine derivatives including uric acid, xanthine, hypoxanthine, guanine, and adenine without surface contamination. By applying a constant potential of -0.125 V (vs. Ag/AgCl) in a flow injection system, the chelating capability of these purine derivatives converts the cuprous oxide layer into a soluble complex. This behavior would dissolve the passive oxide layer and expose the bottom copper layer to the solution, subsequently; an oxidation current which attributed to the regeneration of the original cuprous oxide layer is used to reflect the concentration of these purine derivatives. In a 50mM phosphate buffer, pH 7.0, this approach provides a high sensitivity with LOQ of sub-micro molar level of five purines and high stability with a RSD of 2.5% for 10 μM xanthine (N=12). This method does not suffer from most biological species including ascorbic acid, acetaminophen, creatine, dopamine, sarcosine, ammonium ion, chloride ion, and urea at equal or higher than its physiological concentration.
authors.affiliation: Department of Chemistry, Tamkang University, Tamsui 25137, Taiwan.
authors.forename: Chi-Hao
authors.initials: CH
authors.lastname: Chen
authors.forename: Ya-Ting
authors.initials: YT
authors.lastname: Lin
authors.forename: Meng-Shan
authors.initials: MS
authors.lastname: Lin
mesh_terms: D000225:Adenine / Q000032:analysis; D003300:Copper / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D006147:Guanine / Q000032:analysis; D019271:Hypoxanthine / Q000032:analysis; D057230:Limit of Detection; D010084:Oxidation-Reduction; D011687:Purines / Q000032:analysis*; D014527:Uric Acid / Q000032:analysis; D019820:Xanthine / Q000032:analysis
chemical_list: D011687:Purines; D019820:Xanthine; D014527:Uric Acid; D019271:Hypoxanthine; D006147:Guanine; D003300:Copper; D000225:Adenine; C000520:cuprous oxide
keywords: Adenine; Amperometry; Guanine; Hypoxanthine; Uric acid; Xanthine