title: Distinct roles of LAFL network genes in promoting the embryonic seedling fate in the absence of VAL repression.
abstract: The transition between seed and seedling phases of development is coordinated by an interaction between the closely related ABSCISIC ACID-INSENSITIVE3 (ABI3), FUSCA3 (FUS3), and LEAFY COTYLEDON2 (LEC2; AFL) and VIVIPAROUS1/ABI3-LIKE (VAL) clades of the B3 transcription factor family that respectively activate and repress the seed maturation program. In the val1 val2 double mutant, derepression of the LEC1, LEC1-LIKE (L1L), and AFL (LAFL) network is associated with misexpression of embryonic characteristics resulting in arrested seedling development. We show that while the frequency of the embryonic fate in val1 val2 seedlings depends on the developmental timing of seed rescue, VAL proteins repress LAFL genes during germination, but not during seed development. Quantitative analysis of LAFL mutants that suppress the val1 val2 seedling phenotype revealed distinct roles of LAFL genes in promoting activation of the LAFL network. LEC2 and FUS3 are both essential for coordinate activation of the network, whereas effects of LEC1, L1L, and ABI3 are additive. Suppression of the val1 val2 seedling phenotype by the B3 domain-deficient abi3-12 mutation indicates that ABI3 activation of the LAFL network requires the B3 DNA-binding domain. In the VAL-deficient background, coordinate regulation of the LAFL network is observed over a wide range of genetic and developmental conditions. Our findings highlight distinct functional roles and interactions of LAFL network genes that are uncovered in the absence of VAL repressors.
authors.affiliation: Horticultural Sciences Department, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Jia
authors.forename: Donald R
authors.initials: DR
authors.lastname: McCarty
authors.forename: Masaharu
authors.initials: M
authors.lastname: Suzuki
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks / Q000235:genetics*; D018525:Germination / Q000235:genetics; D009154:Mutation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000235:genetics*; D012639:Seeds / Q000235:genetics* / Q000254:growth & development; D014157:Transcription Factors / Q000235:genetics
chemical_list: C091601:ABI3 protein, Arabidopsis; D029681:Arabidopsis Proteins; C502726:FUSCA3 protein, Arabidopsis; C504240:LEC2 protein, Arabidopsis; D014157:Transcription Factors
references.citation: Plant Cell. 2013 Jan;25(1):134-48
references.pmid: 23362207
references.citation: Plant Cell. 1997 May;9(5):799-807
references.pmid: 9165754
references.citation: Cell Res. 2010 Dec;20(12):1332-44
references.pmid: 21060339
references.citation: Genes Dev. 1995 Oct 15;9(20):2459-69
references.pmid: 7590227
references.citation: Plant Cell. 1994 May;6(5):589-600
references.pmid: 12244252
references.citation: Cell. 1991 Sep 6;66(5):895-905
references.pmid: 1889090
references.citation: Plant Cell Physiol. 1994 Apr;35(3):509-13
references.pmid: 8055176
references.citation: Plant Physiol. 2007 Feb;143(2):902-11
references.pmid: 17158584
references.citation: Plant J. 2001 Jun;26(6):627-35
references.pmid: 11489176
references.citation: Development. 2001 Jan;128(2):243-52
references.pmid: 11124119
references.citation: Plant J. 2009 Jun;58(5):843-56
references.pmid: 19207209
references.citation: Proc Natl Acad Sci U S A. 2007 Feb 13;104(7):2543-7
references.pmid: 17267611
references.citation: Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13839-44
references.pmid: 10570159
references.citation: Plant Physiol. 2010 Feb;152(2):516-28
references.pmid: 19783648
references.citation: Plant Cell. 1997 Aug;9(8):1265-77
references.pmid: 9286105
references.citation: Plant Cell. 1994 Nov;6(11):1567-82
references.pmid: 7827492
references.citation: PLoS Genet. 2009 Aug;5(8):e1000605
references.pmid: 19680533
references.citation: Proc Natl Acad Sci U S A. 2006 Feb 28;103(9):3468-73
references.pmid: 16492731
references.citation: Development. 2003 Dec;130(24):6065-73
references.pmid: 14597573
references.citation: Plant Cell Physiol. 2005 Mar;46(3):399-406
references.pmid: 15695450
references.citation: Plant Physiol. 2013 Mar;161(3):1251-64
references.pmid: 23314941
references.citation: Genetics. 2002 Jul;161(3):1247-55
references.pmid: 12136027
references.citation: Planta. 2012 Jul;236(1):1-17
references.pmid: 22476218
references.citation: Wiley Interdiscip Rev Dev Biol. 2014 Jan-Feb;3(1):135-45
references.pmid: 24902838
references.citation: Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):3151-6
references.pmid: 18287041
references.citation: BMC Res Notes. 2010 May 05;3:126
references.pmid: 20444276
references.citation: PLoS Genet. 2012;8(3):e1002512
references.pmid: 22457632
references.citation: Plant Physiol. 2008 Jan;146(1):149-61
references.pmid: 18024558
references.citation: Plant Cell. 1994 Dec;6(12):1731-1745
references.pmid: 12244233
references.citation: Plant Cell. 2006 Jul;18(7):1642-51
references.pmid: 16731585
references.citation: Plant J. 1998 Sep;15(6):755-64
references.pmid: 9807814
references.citation: Plant J. 2000 Mar;21(5):401-8
references.pmid: 10758492
references.citation: Plant Cell. 1992 Oct;4(10):1251-61
references.pmid: 1359917
references.citation: Plant Cell. 1996 Jul;8(7):1171-9
references.pmid: 8768375
references.citation: EMBO Rep. 2006 Sep;7(9):947-52
references.pmid: 16878125
references.citation: Genes Dev. 1992 Apr;6(4):609-18
references.pmid: 1532784
references.citation: Plant Cell Physiol. 2010 Dec;51(12):2031-46
references.pmid: 21045071
references.citation: Plant Cell. 1994 Aug;6(8):1049-1064
references.pmid: 12244265
references.citation: Plant J. 1997 Dec;12(6):1231-40
references.pmid: 9450338
references.citation: Plant Physiol. 2008 Jul;147(3):1143-57
references.pmid: 18508955
references.citation: Plant J. 2012 Aug;71(3):427-42
references.pmid: 22429691
references.citation: Development. 2011 Jul;138(14):2977-86
references.pmid: 21693514
references.citation: Plant J. 2003 Jul;35(1):33-43
references.pmid: 12834400
references.citation: Planta. 2004 May;219(1):158-66
references.pmid: 14767767
references.citation: Dev Cell. 2004 Sep;7(3):373-85
references.pmid: 15363412
references.citation: Plant Physiol. 2005 Jun;138(2):675-85
references.pmid: 15894743
references.citation: Plant J. 1999 Aug;19(4):371-7
references.pmid: 10504559
references.citation: Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11806-11
references.pmid: 11573014
references.citation: Cell. 1998 Jun 26;93(7):1195-205
references.pmid: 9657152
references.citation: Plant Cell. 2009 Jan;21(1):54-71
references.pmid: 19155348
references.citation: Science. 1997 Jul 4;277(5322):91-4
references.pmid: 9204906
references.citation: Plant Cell. 2003 Jan;15(1):5-18
references.pmid: 12509518
references.citation: Nucleic Acids Res. 2012 Sep 1;40(17):8240-54
references.pmid: 22730287
references.citation: Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15348-53
references.pmid: 10611387
references.citation: Plant J. 1997 Apr;11(4):693-702
references.pmid: 9161030