title: Characterization of a purine permease family gene OsPUP7 involved in growth and development control in rice.
abstract: In this study, PUP-type cytokinin transporter genes were identified in rice (Oryza sativa L.). The Oryza sativa purine permease (OsPUP) family has 12 members that show similar predicted protein sequences with AtPUPs. To reveal the functions of OsPUP genes, we searched the T-DNA mutant library of rice and found one mutant for the member OsPUP7. The T-DNA insertion caused a new transcript that encodes a protein with 26 amino acids different from the native OsPUP7 at the C-terminus. The mutant showed multiple phenotypic changes including increased plant height, big seeds, and delayed flowering. The mutant also showed increased sensitivity to drought and salt stresses and treatments with kinetin and abscisic acid. OsPUP7 is expressed mainly in the vascular bundle, pistil, and stamens. The measurement of cytokinins (CKs) showed that CK content in the mutant spikelets accumulated higher than that in the wild type. Moreover, uptake experiment in the yeast fcy2 mutant suggested that OsPUP7 has the ability to transport caffeine, a CK derivative. Our results indicate that the PUP transport system also exists in rice, and OsPUP7 has an important role in the transport of CK, thus affecting developmental process and stress responses.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Zhuyun
authors.initials: Z
authors.lastname: Qi
authors.forename: Lizhong
authors.initials: L
authors.lastname: Xiong
mesh_terms: D003583:Cytokinins / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics*; D005810:Multigene Family; D016254:Mutagenesis, Insertional / Q000235:genetics; D009154:Mutation / Q000235:genetics; D033702:Nucleobase Transport Proteins / Q000235:genetics* / Q000378:metabolism; D012275:Oryza / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D010641:Phenotype; D063245:Plant Development / Q000187:drug effects / Q000235:genetics*; D010937:Plant Growth Regulators / Q000494:pharmacology; D021381:Protein Transport / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics
chemical_list: D003583:Cytokinins; D004269:DNA, Bacterial; D033702:Nucleobase Transport Proteins; D010937:Plant Growth Regulators; D012333:RNA, Messenger; C036483:T-DNA; C022483:purine permease; D012965:Sodium Chloride
keywords: Abiotic stress; cytokinin; purine permease; rice; transporter