title: The mouse digit tip: from wound healing to regeneration.
abstract: A challenge to the study of regeneration is determining at what point the processes of wound healing and regeneration diverge. The mouse displays level-specific regeneration responses. An amputation through the distal third of the terminal phalanx will prompt a regeneration response and result in a new digit tip that mimics the morphology of the lost digit tip. Conversely, an amputation through the distal third of the intermediate phalanx initiates a wound healing and scarring response. The mouse, therefore, provides a model for studying the transition between wound healing and regeneration in the same animal. This chapter details the methods used in the study of mammalian digit regeneration, including a method to introduce exogenous protein into the mouse digit amputation model via microcarrier beads and methods for analysis of bone regeneration.
authors.affiliation: Division of Cell and Molecular Biology, Tulane University, New Orleans, LA, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Simkin
authors.forename: Manjong
authors.initials: M
authors.lastname: Han
authors.forename: Ling
authors.initials: L
authors.lastname: Yu
authors.forename: Mingquan
authors.initials: M
authors.lastname: Yan
authors.forename: Ken
authors.initials: K
authors.lastname: Muneoka
mesh_terms: D000671:Amputation, Surgical / Q000379:methods; D000818:Animals; D005121:Extremities / Q000601:surgery; D005260:Female; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D051379:Mice; D023421:Models, Animal; D012038:Regeneration / Q000502:physiology*; D014945:Wound Healing / Q000502:physiology*