title: Production of monoclonal antibody against ORF72 of koi herpesvirus isolated in Taiwan.
abstract: A monoclonal antibody (MAb) was generated against the capsid protein (ORF 72) of koi herpesvirus (KHV) isolated from diseased koi Cyprinus carpio in Taiwan. The clone of MAb-B2 was obtained by immunizing mice with whole virus particles and further identified using indirect enzyme-linked immunosorbent assay and Western blot assay. In addition, it detected KHV in KHV-infected cells but not in those of mock-infected cells as demonstrated by indirect immunofluorescence assay. The neutralization test showed that MAb-B2 neutralized KHV. Furthermore, we uncovered that MAb-B2 recognizes the ORF72 of KHV as revealed by liquid chromatography-tandem mass spectrometry and Western blot assays. Additionally, MAb-B2 has been used as a diagnostic tool for detection of KHV in clinical samples by immunohistochemistry. Collectively, our results indicated that MAb-B2 could be used in the development of a diagnostic kit for diagnosis of KHV infections and ORF72 protein of KHV might be a candidate for future vaccine development.
authors.affiliation: Animal Health Research Institute, Council of Agriculture, Executive Yuan No. 376, Chung-Cheng Road, Tansui, New Taipei City, 25158, Taiwan.
authors.forename: Chien
authors.initials: C
authors.lastname: Tu
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Lu
authors.forename: Chia-Yu
authors.initials: CY
authors.lastname: Hsieh
authors.forename: Su-Ming
authors.initials: SM
authors.lastname: Huang
authors.forename: Shao-Kuang
authors.initials: SK
authors.lastname: Chang
authors.forename: Meei-Mei
authors.initials: MM
authors.lastname: Chen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D057134:Antibodies, Neutralizing / Q000276:immunology* / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000276:immunology* / Q000302:isolation & purification; D000956:Antigens, Viral / Q000276:immunology*; D015153:Blotting, Western; D036022:Capsid Proteins / Q000276:immunology*; D002347:Carps / Q000821:virology*; D002853:Chromatography, Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006564:Herpesviridae / Q000276:immunology* / Q000302:isolation & purification; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D013624:Taiwan; D053719:Tandem Mass Spectrometry
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins
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