title: Mycoplasmas hyorhinis in different regions of cuba. diagnosis.
abstract: M. hyorhinis is considered one of the etiological agents of arthritis in sucking pigs, but recently as seen, some strains can produce pneumonia that could not be distinguished from the mycoplasmosis caused by M. hyopneumoniae. The study was conducted to research the presence of Mycoplasma hyorhinis (M. hyorhinis ) in different regions of the country from exudates of pig lungs with typical EP lesions. Exudates from 280 pig lungs with typical EP lesions were studied using molecular techniques such as PCR, real time PCR and amplification of the 16S-23S rRNA. It was detected that the 66% of the samples studied resulted positive to M. hyorhinis, and the presence of this species was detected in all the provinces. Amplification and studies on the intergenic region 16S-23S of M. hyorhinis rRNA demonstrated the existing variability among strains of a same species. This study is the first report on M. hyorhinis detection in Cuba.
authors.affiliation: Laboratorio de Diagnóstico de Micoplasmas (MYCOLAB), grupo de Biología Molecular, Dirección de Microbiología , CENSA , Cuba;
authors.forename: Evelyn
authors.initials: E
authors.lastname: Lobo
authors.forename: Carlos
authors.initials: C
authors.lastname: Poveda
authors.forename: Rakesh
authors.initials: R
authors.lastname: Gupta
authors.forename: Alejandro
authors.initials: A
authors.lastname: Suarez
authors.forename: Yenney
authors.initials: Y
authors.lastname: Hernández
authors.forename: Ana
authors.initials: A
authors.lastname: Ramírez
authors.forename: José B
authors.initials: JB
authors.lastname: Poveda
keywords: PCR; diagnostic; enzootic pneumonia; mycoplasma
references.citation: Avian Dis. 2007 Jun;51(2):555-60
references.pmid: 17626483
references.citation: Vet Microbiol. 2006 Jun 15;115(1-3):111-6
references.pmid: 16540266
references.citation: J Clin Microbiol. 2008 Aug;46(8):2491-8
references.pmid: 18524960
references.citation: Methods Mol Biol. 1998;104:145-65
references.pmid: 9711651
references.citation: Acta Vet Scand. 1971;12(1):116-9
references.pmid: 5103408
references.citation: Vet Rec. 2008 Mar 1;162(9):267-71
references.pmid: 18310558
references.citation: J Clin Microbiol. 2000 Apr;38(4):1390-6
references.pmid: 10747113
references.citation: Vet Microbiol. 2000 Oct 1;76(3):299-303
references.pmid: 10973704
references.citation: Vet Microbiol. 2008 Apr 30;128(3-4):279-87
references.pmid: 18055138
references.citation: Vet Q. 1996 Sep;18(3):104-9
references.pmid: 8903144
references.citation: Appl Microbiol Biotechnol. 2006 Aug;71(5):680-98
references.pmid: 16470366
references.citation: Int J Syst Evol Microbiol. 2007 Nov;57(Pt 11):2703-2719
references.pmid: 17978244
references.citation: Vet J. 2008 Feb;175(2):273-5
references.pmid: 17339121
references.citation: J Am Vet Med Assoc. 1994 Jan 1;204(1):102-7
references.pmid: 8125807