title: Expression of phenazine biosynthetic genes during the arbuscular mycorrhizal symbiosis of Glomus intraradices.
abstract: To explore the molecular mechanisms that prevail during the establishment of the arbuscular mycorrhiza symbiosis involving the genus Glomus, we transcriptionally analysed spores of Glomus intraradices BE3 during early hyphal growth. Among 458 transcripts initially identified as being expressed at presymbiotic stages, 20% of sequences had homology to previously characterized eukaryotic genes, 30% were homologous to fungal coding sequences, and 9% showed homology to previously characterized bacterial genes. Among them, GintPbr1a encodes a homolog to Phenazine Biosynthesis Regulator (Pbr) of Burkholderia cenocepacia, an pleiotropic regulatory protein that activates phenazine production through transcriptional activation of the protein D isochorismatase biosynthetic enzyme phzD (Ramos et al., 2010). Whereas GintPbr1a is expressed during the presymbiotic phase, the G. intraradices BE3 homolog of phzD (BGintphzD) is transcriptionally active at the time of the establishment of the arbuscular mycorrhizal symbiosis. DNA from isolated bacterial cultures found in spores of G. intraradices BE3 confirmed that both BGintPbr1a and BGintphzD are present in the genome of its potential endosymbionts. Taken together, our results indicate that spores of G. intraradices BE3 express bacterial phenazine biosynthetic genes at the onset of the fungal-plant symbiotic interaction.
authors.affiliation: Laboratorio de Bioquímica Ecológica. Centro de Investigación y Estudios Avanzados del IPN. CP 36821 , Irapuato Guanajuato , México ; Grupo de Desarrollo Reproductivo y Apomixis. Departamento de Ingeniería Genética de Plantas y Laboratorio Nacional de Genómica para la Biodiversidad , Centro de Investigación y Estudios Avanzados, CP 36821, Irapuato Guanajuato , México.
authors.forename: Dionicia Gloria
authors.initials: DG
authors.lastname: León-Martínez
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Vielle-Calzada
authors.forename: Víctor
authors.initials: V
authors.lastname: Olalde-Portugal
keywords: Glomus intradices; biosynthesis; mycorrhizal fungi; phenazine
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