title: Reverse-transcription PCR (RT-PCR).
abstract: RT-PCR is commonly used to test for genetic diseases and to characterize gene expression in various tissue types, cell types, and over developmental time courses. This serves as a form of expression profiling, but typically as a candidate approach. RT-PCR is also commonly used to clone cDNAs for further use with other molecular biology techniques (e.g., see Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates and Circularized RT-PCR (cRT-PCR): analysis of RNA 5' ends, 3' ends, and poly(A) tails).
authors.affiliation: Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: jbachma9@gmail.com.
authors.forename: Julia
authors.initials: J
authors.lastname: Bachman
mesh_terms: D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D012313:RNA / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D048348:Reverse Transcription
chemical_list: D018076:DNA, Complementary; D012313:RNA
keywords: Amplifying/cloning products; Polymerase chain reaction (PCR); Reverse transcriptase enzyme; Reverse-transcription PCR (RT-PCR); Unwinding RNA