title: Microfluidic-integrated biosensors: prospects for point-of-care diagnostics.
abstract: There is a growing demand to integrate biosensors with microfluidics to provide miniaturized platforms with many favorable properties, such as reduced sample volume, decreased processing time, low cost analysis and low reagent consumption. These microfluidics-integrated biosensors would also have numerous advantages such as laminar flow, minimal handling of hazardous materials, multiple sample detection in parallel, portability and versatility in design. Microfluidics involves the science and technology of manipulation of fluids at the micro- to nano-liter level. It is predicted that combining biosensors with microfluidic chips will yield enhanced analytical capability, and widen the possibilities for applications in clinical diagnostics. The recent developments in microfluidics have helped researchers working in industries and educational institutes to adopt some of these platforms for point-of-care (POC) diagnostics. This review focuses on the latest advancements in the fields of microfluidic biosensing technologies, and on the challenges and possible solutions for translation of this technology for POC diagnostic applications. We also discuss the fabrication techniques required for developing microfluidic-integrated biosensors, recently reported biomarkers, and the prospects of POC diagnostics in the medical industry.
authors.affiliation: Department of Biotechnology, Delhi Technological University, Shahbad Daulatpur, Delhi, India.
authors.forename: Suveen
authors.initials: S
authors.lastname: Kumar
authors.forename: Saurabh
authors.initials: S
authors.lastname: Kumar
authors.forename: Md Azahar
authors.initials: MA
authors.lastname: Ali
authors.forename: Pinki
authors.initials: P
authors.lastname: Anand
authors.forename: Ved Varun
authors.initials: VV
authors.lastname: Agrawal
authors.forename: Renu
authors.initials: R
authors.lastname: John
authors.forename: Sagar
authors.initials: S
authors.lastname: Maji
authors.forename: Bansi D
authors.initials: BD
authors.lastname: Malhotra
mesh_terms: D015415:Biomarkers; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D019095:Point-of-Care Systems
chemical_list: D015415:Biomarkers
keywords: Biomarker; Biosensor; Microfluidics; Point-of-care diagnostic