title: Sandwich assay for mixed-sequence recognition of double-stranded DNA: invader-based detection of targets specific to foodborne pathogens.
abstract: A 96-well plate sandwich assay based on Invader capture/signalling probes is used to recognize 28-mer mixed-sequence dsDNA targets specific to Salmonella enterica, Campylobacter jejuni, Escherichia coli. Targets are detected down to 20-55 pM concentration with excellent binding specificity.
authors.affiliation: Department of Chemistry, University of Idaho, 875 Perimeter Drive MS 2343, Moscow, ID 83844-2343, USA. hrdlicka@uidaho.edu.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Denn
authors.forename: Saswata
authors.initials: S
authors.lastname: Karmakar
authors.forename: Dale C
authors.initials: DC
authors.lastname: Guenther
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Hrdlicka
mesh_terms: D001665:Binding Sites; D016123:Campylobacter jejuni / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D004269:DNA, Bacterial / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000737:chemistry*; D019779:Salmonella enterica / Q000737:chemistry*
chemical_list: D004269:DNA, Bacterial; D004247:DNA
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