title: Identifying and characterizing Yarrowia keelungensis sp. nov., an oil-degrading yeast isolated from the sea surface microlayer.
abstract: Ascomycetous yeast strain SM-22 was isolated from the sea-surface microlayer near the Keelung City off the northern coast of Taiwan. This strain showed a cell surface hydrophobicity higher than 90 %, moderate UV A/B resistance, and it degraded 68 % of the total petroleum hydrocarbon content of an artificial seawater medium containing 1 % (v v(-1)) diesel oil within 15 days at 25 °C. The closest phylogenetic relative of this strain is Candida oslonensis CBS 10146(T), but it differs from strain SM-22 by a 3.7 % divergence (including 18 nucleotide substitutions and 2 gaps) in the D1/D2 domain sequence of the large subunit rRNA gene. This difference clearly suggests that the strain SM-22 represents a distinct species. Strain SM-22 does not produce ascospores on common sporulation media and it can therefore be considered an anamorph of the genus Yarrowia. Thus, the name Yarrowia keelungensis sp. nov. (type strain SM-22(T) = BCRC 23110(T) = JCM 14894(T) = CBS 11062(T)) is proposed as a novel species of genus Yarrowia.
authors.affiliation: Graduate Institute of Marine Biology, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung, 20224, Taiwan.
authors.forename: Chin-Feng
authors.initials: CF
authors.lastname: Chang
authors.forename: Chih-Chen
authors.initials: CC
authors.lastname: Chen
authors.forename: Ching-Fu
authors.initials: CF
authors.lastname: Lee
authors.forename: Shiu-Mei
authors.initials: SM
authors.lastname: Liu
mesh_terms: D001711:Biotransformation; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D008853:Microscopy; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D009821:Oils / Q000378:metabolism*; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013172:Spores, Fungal / Q000254:growth & development; D013624:Taiwan; D025062:Yarrowia / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D003470:Culture Media; D004271:DNA, Fungal; D021901:DNA, Intergenic; D004275:DNA, Ribosomal; D009821:Oils; D012331:RNA, Fungal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S