title: Balanced production of ribosome components is required for proper G1/S transition in Saccharomyces cerevisiae.
abstract: Cell cycle regulation is a very accurate process that ensures cell viability and the genomic integrity of daughter cells. A fundamental part of this regulation consists in the arrest of the cycle at particular points to ensure the completion of a previous event, to repair cellular damage, or to avoid progression in potentially risky situations. In this work, we demonstrate that a reduction in nucleotide levels or the depletion of RNA polymerase I or III subunits generates a cell cycle delay at the G1/S transition in Saccharomyces cerevisiae. This delay is concomitant with an imbalance between ribosomal RNAs and proteins which, among others, provokes an accumulation of free ribosomal protein L5. Consistently with a direct impact of free L5 on the G1/S transition, rrs1 mutants, which weaken the assembly of L5 and L11 on pre-60S ribosomal particles, enhance both the G1/S delay and the accumulation of free ribosomal protein L5. We propose the existence of a surveillance mechanism that couples the balanced production of yeast ribosomal components and cell cycle progression through the accumulation of free ribosomal proteins. This regulatory pathway resembles the p53-dependent nucleolar-stress checkpoint response described in human cells, which indicates that this is a general control strategy extended throughout eukaryotes.
authors.affiliation: From the Departamento de Genética, Universidad de Sevilla and Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, 41012 Sevilla, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Gómez-Herreros
authors.forename: Olga
authors.initials: O
authors.lastname: Rodríguez-Galán
authors.forename: Macarena
authors.initials: M
authors.lastname: Morillo-Huesca
authors.forename: Douglas
authors.initials: D
authors.lastname: Maya
authors.forename: María
authors.initials: M
authors.lastname: Arista-Romero
authors.forename: Jesús
authors.initials: J
authors.lastname: de la Cruz
authors.forename: Sebastián
authors.initials: S
authors.lastname: Chávez
authors.forename: Mari Cruz
authors.initials: MC
authors.lastname: Muñoz-Centeno
mesh_terms: D016193:G1 Phase / Q000502:physiology*; D006801:Humans; D012318:RNA Polymerase I / Q000235:genetics / Q000378:metabolism; D012319:RNA Polymerase II / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000096:biosynthesis* / Q000235:genetics; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism*; D016196:S Phase / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; C027624:ribosomal protein L11; D012319:RNA Polymerase II; D012318:RNA Polymerase I
keywords: Cell Cycle; Free Ribosomal Proteins; G1/S Transition; RNA Polymerase I; RNA Polymerase II; RNA Polymerase III; Ribosomal RNA (rRNA); Ribosome Assembly; Ribosomes; Transcription
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