title: Constitutive and inducible pectinolytic enzymes from Aspergillus flavipes FP-500 and their modulation by pH and carbon source.
abstract: Growth and enzymes production by Aspergillus flavipes FP-500 were evaluated on pectin, polygalacturonic acid, galacturonic acid, arabinose, rhamnose, xylose, glycerol and glucose at different initial pH values. We found that the strain produced exopectinases, endopectinases and pectin lyases. Exopectinases and pectin lyase were found to be produced at basal levels as constitutive enzymes and their production was modulated by the available carbon source and pH of culture medium and stimulated by the presence of inducer in the culture medium. Endo-pectinase was basically inducible and was only produced when pectin was used as carbon source. Our results suggest that pectinases in A. flavipes FP-500 are produced in a concerted way. The first enzyme to be produced was exopectinase followed by Pectin Lyase and Endo-pectinase.
authors.affiliation: Laboratory of Enzymatic Catalysis, Technologic Institute for Higher Studies of Ecatepec , Ecatepec , Estado de Mexico ; Department of Bioengineering, Professional Unit of Biotechnology, National Polytechnic Institute of Mexico, UPIBI-IPN, Col. La Laguna Ticoman, D.F. , Mexico.
authors.forename: Aurora
authors.initials: A
authors.lastname: Martínez-Trujillo
authors.forename: Juan S
authors.initials: JS
authors.lastname: Aranda
authors.forename: Carlos
authors.initials: C
authors.lastname: Gómez-Sánchez
authors.forename: Blanca
authors.initials: B
authors.lastname: Trejo-Aguilar
authors.forename: Guillermo
authors.initials: G
authors.lastname: Aguilar-Osorio
keywords: Aspergillus flavipes; constitutive enzymes; inducible enzymes; pectin; pectinolytic enzymes
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