title: Potassium ion-dependent trehalose phosphorylase from halophilic Bacillus selenitireducens MLS10.
abstract: We discovered a potassium ion-dependent trehalose phosphorylase (Bsel_1207) belonging to glycoside hydrolase family 65 from halophilic Bacillus selenitireducens MLS10. Under high potassium ion concentrations, the recombinant Bsel_1207 produced in Escherichia coli existed as an active dimeric form that catalyzed the reversible phosphorolysis of trehalose in a typical sequential bi bi mechanism releasing β-D-glucose 1-phosphate and D-glucose. Decreasing potassium ion concentrations significantly reduced thermal and pH stabilities, leading to formation of inactive monomeric Bsel_1207.
authors.affiliation: Graduate School of Science and Technology, Niigata University, Niigata 950-2181, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Nihira
authors.forename: Yuka
authors.initials: Y
authors.lastname: Saito
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Chiku
authors.forename: Motomitsu
authors.initials: M
authors.lastname: Kitaoka
authors.forename: Ken'ichi
authors.initials: K
authors.lastname: Ohtsubo
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakai
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D016680:Genome, Bacterial; D005964:Glucosyltransferases / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009995:Osmosis; D011188:Potassium / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D005964:Glucosyltransferases; C023181:alpha,alpha-trehalose phosphorylase; D011188:Potassium
keywords: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; ABC; ATP-binding cassette; Bacillus selenitireducens; GH; Glycoside hydrolase family 65; HEPES; PAGE; PTS; Potassium ion-dependent; Trehalose phosphorylase; glycoside hydrolase family; phosphoenolpyruvate: carbohydrate phosphotransferase; polyacrylamide-gel electrophoresis; β-d-glucose 1-phosphate; βGlc1P