title: In vitro effectiveness of anidulafungin against Candida sp. biofilms.
abstract: This study furnishes deeper insights to previous works on anidulafungin, demonstrating the potent activity against Candida strains planktonic cells and biofilms. Candida sp., associated with many biomaterial-related infections, give rise to infective pathologies typically associated with biofilm formation. We recently determined the in vitro antifungal activities of echinocandin anidulafungin in association with some antifungal drugs against some Candida strains in their planktonic states. A total of 11 Candida strains biofilms were tested in this study: six Candida albicans, three C. parapsilosis and two C. tropicalis. All yeast isolates and ATCC strains were stored at -20°C in glycerol stocks and were subcultured on antimicrobial agent-free Sabouraud dextrose agar plates. MIC endpoints were determined colorimetrically by using the indicator 2,3-bis(2-methoxy-4-nitro-5-sulphophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) with menadione as electron-coupling agent. The activity of anidulafungin was assessed using in vitro microbiological model relevant for clinical practice. Anidulafungin showed a strong activity in vitro against both planktonic and biofilms cells, and our study confirms that high anidulafungin concentrations might establish paradoxical growth effect in C. albicans and C. tropicalis biofilms.
authors.affiliation: Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari 'Aldo Moro', Via Orabona 4, Bari Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Rosato
authors.affiliation: Scuola di Dottorato e Scienze Biomediche e Oncologia umana, Sezione di Igiene, Università di Bari 'Aldo Moro', Bari, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Piarulli
authors.affiliation: Scuola di Dottorato e Scienze Biomediche e Oncologia umana, Sezione di Igiene, Università di Bari 'Aldo Moro', Bari, Italy.
authors.forename: Brigida Pia Immacolata
authors.initials: BP
authors.lastname: Schiavone
authors.affiliation: Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari 'Aldo Moro', Via Orabona 4, Bari Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Catalano
authors.affiliation: Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari 'Aldo Moro', Via Orabona 4, Bari Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Carocci
authors.affiliation: Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari 'Aldo Moro', Via Orabona 4, Bari Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Carrieri
authors.affiliation: Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari 'Aldo Moro', Via Orabona 4, Bari Italy.
authors.forename: Addolorata
authors.initials: A
authors.lastname: Carone
authors.affiliation: Dipartimento di Scienze Biomediche e Oncologia umana, Sezione di Igiene, Università di Bari 'Aldo Moro', Bari, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Caggiano
authors.affiliation: Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari 'Aldo Moro', Via Orabona 4, Bari Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Franchini
authors.affiliation: Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari 'Aldo Moro', Via Orabona 4, Bari Italy.
authors.forename: Filomena
authors.initials: F
authors.lastname: Corbo
authors.affiliation: Dipartimento di Scienze Biomediche e Oncologia umana, Sezione di Igiene, Università di Bari 'Aldo Moro', Bari, Italy.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Montagna
mesh_terms: D000077612:Anidulafungin; D000935:Antifungal Agents / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002175:Candida / Q000187:drug effects* / Q000254:growth & development; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development; D041022:Candida tropicalis / Q000187:drug effects / Q000254:growth & development; D002177:Candidiasis / Q000517:prevention & control; D055499:Catheter-Related Infections / Q000517:prevention & control; D054714:Echinocandins / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D013045:Species Specificity
chemical_list: D000935:Antifungal Agents; D054714:Echinocandins; D000077612:Anidulafungin