title: Fabrication of poly(lactic-co-glycolic acid) scaffolds containing silk fibroin scaffolds for tissue engineering applications.
abstract: The present study deals with the fabrication of poly(lactic-co-glycolic acid) (PLGA) scaffolds modified with silk fibroin for biomedical application. The PLGA solutions were added with salt particles and pressed with high pressures; which were further subjected to salt leaching resulting in the creation of large sized pores in the PLGA scaffolds. To fill up these pores, 2%, 4%, and 8% of silk solutions were added, however, the addition created extra small sized pores. The scaffolds were characterized by various state of techniques; the scanning electronic microscopy revealed the large sized pores in the pristine scaffold can be tailored into smaller architecture by the addition of silk fibroin. The contact angle measurements confirmed the introduction of silk helped to change the hydrophobic nature of PLGA into hydrophilic, which is the main constrain for PLGA. The mechanical properties of scaffold can be easily improved by applying the higher amounts of silk into the scaffolds. The thermal gravimetric analyses and fourier transform infrared spectroscopy confirmed the presence of silk fibroin in scaffolds. The cell viability and cell attachment was checked by culturing the scaffolds with NIH 3T3 fibroblasts and chondrocytes. Furthermore, these results revealed that the introduction of silk had significant impact on the viability of fibroblast also had a good affinity for cell attachment and infiltration of human chondrocytes in scaffolds after culturing the cells for 2 and 5 weeks of time.
authors.affiliation: Nano-Bio Regenerative Medical Institute, Collage of Medicine, Hallym University, Chuncheon, 200-702, South Korea.
authors.forename: Hyung Woo
authors.initials: HW
authors.lastname: Ju
authors.forename: Faheem A
authors.initials: FA
authors.lastname: Sheikh
authors.forename: Bo Mi
authors.initials: BM
authors.lastname: Moon
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Park
authors.forename: Ok Joo
authors.initials: OJ
authors.lastname: Lee
authors.forename: Jung Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Jang Ji
authors.initials: JJ
authors.lastname: Eun
authors.forename: Gilson
authors.initials: G
authors.lastname: Khang
authors.forename: Chan Hum
authors.initials: CH
authors.lastname: Park
mesh_terms: D000818:Animals; D012831:Bombyx; D002448:Cell Adhesion; D002470:Cell Survival; D019902:Chondrocytes / Q000166:cytology / Q000648:ultrastructure; D005347:Fibroblasts / Q000166:cytology / Q000648:ultrastructure; D005349:Fibroins / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013314:Stress, Mechanical; D013818:Thermogravimetry; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D005349:Fibroins
keywords: PLGA; cell viability; fibroblasts; hydrophilic; silk