title: Seed shattering in a wild sorghum is conferred by a locus unrelated to domestication.
abstract: Suppression of seed shattering was a key step during crop domestication that we have previously suggested to be convergent among independent cereal lineages. Positional, association, expression, and mutant complementation data all implicate a WRKY transcription factor, SpWRKY, in conferring shattering to a wild sorghum relative, Sorghum propinquum. We hypothesize that SpWRKY functions in a manner analogous to Medicago and Arabidopsis homologs that regulate cell wall biosynthesis genes, with low expression toward the end of floral development derepressing downstream cell wall biosynthesis genes to allow deposition of lignin that initiates the abscission zone in the seed-pedicel junction. The recent discovery of a YABBY locus that confers shattering within Sorghum bicolor and other cereals validated our prior hypothesis that some parallel domestication may have been convergent. Ironically, however, the shattering allele of SpWRKY appears to be recently evolved in S. propinquum and illustrates a case in which the genetic control of a trait in a wild relative fails to extrapolate even to closely related crops. Remarkably, the SpWRKY and YABBY loci lie only 300 kb apart and may have appeared to be a single genetic locus in some sorghum populations.
authors.affiliation: Plant Genome Mapping Laboratory, University of Georgia, Athens, GA 30605.
authors.forename: Haibao
authors.initials: H
authors.lastname: Tang
authors.forename: Hugo E
authors.initials: HE
authors.lastname: Cuevas
authors.forename: Sayan
authors.initials: S
authors.lastname: Das
authors.forename: Uzay U
authors.initials: UU
authors.lastname: Sezen
authors.forename: Chengbo
authors.initials: C
authors.lastname: Zhou
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.forename: Valorie H
authors.initials: VH
authors.lastname: Goff
authors.forename: Zhengxiang
authors.initials: Z
authors.lastname: Ge
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Clemente
authors.forename: Andrew H
authors.initials: AH
authors.lastname: Paterson
mesh_terms: D000595:Amino Acid Sequence; D018556:Crops, Agricultural / Q000235:genetics* / Q000502:physiology*; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D056726:Genetic Association Studies; D005816:Genetic Complementation Test; D056426:Genetic Loci / Q000235:genetics*; D018745:Genome, Plant / Q000235:genetics; D008031:Lignin / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D020161:Physical Chromosome Mapping; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D019655:Quantitative Trait, Heritable; D012639:Seeds / Q000235:genetics* / Q000502:physiology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D045868:Sorghum / Q000235:genetics* / Q000502:physiology*; D013718:Tensile Strength
chemical_list: D010455:Peptides; D010940:Plant Proteins; D008031:Lignin
keywords: association mapping; cereal crop; seed dispersal
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