title: Development of a membrane-based microwave-mediated electrochemical ELISA method for TNF-α detection in patients with periodontitis.
abstract: The tumor necrosis factor-α (TNF-α) is implicated in periodontal disease, and there was an attempt to quantitate it by a membrane-based microwave-mediated electrochemical enzyme-linked immunosorbent assay (MMeELISA) using p-aminophenyl phosphate (pAPP) with an over-all time of 1.5 h. A differential pulse voltammetric signal increased linearly with an increase in the amount of TNF-α with a detection limit of 0.48 pg mL(-1). This bio-sensing platform revealed a significant difference in the TNF-α level between GCF samples from periodontal patients and healthy volunteers.
authors.affiliation: Research Center for Bio-microsensing Technology, Kyushu Institute of Technology.
authors.forename: Irmina
authors.initials: I
authors.lastname: Diala
authors.forename: Shinobu
authors.initials: S
authors.lastname: Sato
authors.forename: Michihiko
authors.initials: M
authors.lastname: Usui
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nakashima
authors.forename: Tatsuji
authors.initials: T
authors.lastname: Nishihara
authors.forename: Shigeori
authors.initials: S
authors.lastname: Takenaka
mesh_terms: D000328:Adult; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D055664:Electrochemical Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry; D006801:Humans; D008297:Male; D008872:Microwaves; D008875:Middle Aged; D010518:Periodontitis / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
chemical_list: D014409:Tumor Necrosis Factor-alpha; C020253:cellulose ester plastic; D002482:Cellulose