title: Effects of voluntary alcohol drinking on corticotropin-releasing factor and preprodynorphin mRNA levels in the central amygdala of Sardinian alcohol-preferring rats.
abstract: The stress-response corticotropin-releasing factor (CRF) and dynorphin systems are critically involved in alcohol drinking and "anxiety"-related behaviors. Selectively bred Sardinian alcohol-preferring (sP) rats display high inherent "anxiety"-related behaviors, in comparison with their alcohol-nonpreferring counterpart (sNP rats). The present study was undertaken to investigate: (1) if there were genetically determined differences in basal gene expression levels of CRF, CRF-R1, preprodynorphin (ppDyn) and kappa opioid receptor (KOP-r) between sP and sNP rats; specifically, mRNA levels of the above genes were measured in the central amygdala (CeA), hypothalamus and other stress responsive and mesolimbic regions of alcohol-naive sP and sNP rats; and (2) if the above mRNA levels were altered by voluntary alcohol drinking in sP rats exposed to the standard, homecage 2-bottle "alcohol vs. water" choice regimen 24h/day for 17 days. Higher basal CRF mRNA levels were found only in CeA of alcohol-naive sP rats, compared with sNP rats; these levels were decreased after alcohol consumption. In contrast, ppDyn mRNA levels in CeA of sP rats were increased by alcohol consumption, but with no basal difference from sNP rats. Although higher basal ppDyn mRNA levels were found in hypothalamus of sP rats, compared with sNP rats, there was no alteration after alcohol drinking in sP rats. No difference for the above mRNA levels was observed in other regions, including nucleus accumbens shell or core, caudate-putamen, ventral tegmental area and medial/basolateral amygdala, between the two rat lines before or after alcohol consumption. Our results demonstrate the existence of genetically determined high basal CRF mRNA levels in CeA of sP rats. Alcohol consumption decreased CeA CRF mRNA levels with parallel increases in CeA ppDyn mRNA levels.
authors.affiliation: Laboratory of the Biology of Addictive Diseases, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA. Electronic address: yan.zhou@rockefeller.edu.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Colombo
authors.forename: Gian Luigi
authors.initials: GL
authors.lastname: Gessa
authors.forename: Mary Jeanne
authors.initials: MJ
authors.lastname: Kreek
mesh_terms: D000428:Alcohol Drinking / Q000235:genetics / Q000378:metabolism*; D000679:Amygdala / Q000378:metabolism*; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism*; D004399:Dynorphins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009714:Nucleus Accumbens / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D011699:Putamen / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D018019:Receptors, Corticotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism; D017473:Receptors, Opioid, kappa / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D017557:Ventral Tegmental Area / Q000378:metabolism; D000099052:CRF Receptor, Type 1
chemical_list: D011498:Protein Precursors; D012333:RNA, Messenger; D018019:Receptors, Corticotropin-Releasing Hormone; D017473:Receptors, Opioid, kappa; C036448:pre-prodynorphin; D000099052:CRF Receptor, Type 1; D004399:Dynorphins; D003346:Corticotropin-Releasing Hormone
keywords: Alcohol drinking; Central amygdala; Corticotropin-releasing factor; Gene expression; Preprodynorphin; Sardinian alcohol-preferring (sP) and -nonpreferring (sNP) rats
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