title: Effect of F68, F127, and P85 pluronic block copolymers on odontogenic differentiation of human tooth germ stem cells.
abstract: BACKGROUND
  The major challenge in dental pulp engineering is to make a successful combination of stem cells and biomaterials with the aim of providing the differentiation of stem cells into odontogenic cell types. Among biomaterials, some types of pluronics have been reported to increase bone formation of stem cells. The effect of these pluronics on odontogenic differentiation has not been addressed yet. This study aimed to examine the effect of pluronics F68, F127, and P85 on odontogenic differentiation of stem cells derived from third molar tooth germs of young adults.
  
  
  METHODS
  Human tooth germ stem cells (hTGSCs) were induced to differentiate into odontogenic cells in the presence of different concentrations of pluronics. Differentiation efficiency was assessed by quantitative real-time polymerase chain reaction for determining expression messenger RNA levels and by immunocytostaining for determining the protein expression of odontogenic markers (ie, dentin sialoprotein, dentin matrix protein 1, bone morphogenic protein 2, bone morphogenic protein 7) by measuring alkaline phosphatase enzyme activity and lastly by von Kossa staining for determining mineralization.
  
  
  RESULTS
  The results revealed for the first time that F68 has a great potential to boost odontogenic differentiation of hTGSCs. P85 was found to reduce cell viability during differentiation. F127 was nontoxic to hTGSCs but did not have any effect on differentiation.
  
  
  CONCLUSIONS
  The positive effect of F68 on odontogenic differentiation might enable more efficient pulp regeneration. Yet, the exact mechanism of how F68 alters the differentiation pattern of hTGSCs remains to be investigated in the future studies.
authors.affiliation: Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Istanbul, Turkey.
authors.forename: Pakize Neslihan
authors.initials: PN
authors.lastname: Taşlı
authors.forename: Mehmet Emir
authors.initials: ME
authors.lastname: Yalvaç
authors.forename: Nesimi
authors.initials: N
authors.lastname: Sofiev
authors.forename: Fikrettin
authors.initials: F
authors.lastname: Sahin
mesh_terms: D000293:Adolescent; D000469:Alkaline Phosphatase / Q000032:analysis / Q000187:drug effects; D055396:Bone Morphogenetic Protein 2 / Q000032:analysis / Q000187:drug effects; D055419:Bone Morphogenetic Protein 7 / Q000032:analysis / Q000187:drug effects; D002113:Calcification, Physiologic / Q000187:drug effects; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D024042:Collagen Type I / Q000032:analysis / Q000187:drug effects; D000090602:Collagen Type I, alpha 1 Chain; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000187:drug effects; D006801:Humans; D008964:Molar, Third / Q000166:cytology; D009805:Odontogenesis / Q000187:drug effects*; D010750:Phosphoproteins / Q000032:analysis / Q000187:drug effects; D011060:Poloxalene / Q000494:pharmacology*; D020442:Poloxamer / Q000494:pharmacology*; D012795:Sialoglycoproteins / Q000032:analysis / Q000187:drug effects; D013234:Stem Cells / Q000187:drug effects*; D014083:Tooth Germ / Q000166:cytology*; D000099311:Dentin Sialophosphoprotein
chemical_list: D000469:Alkaline Phosphatase; D055396:Bone Morphogenetic Protein 2; D055419:Bone Morphogenetic Protein 7; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D011060:Poloxalene; D020442:Poloxamer; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C525718:BMP2 protein, human; C525819:BMP7 protein, human; C513309:DMP1 protein, human; C422648:pluronic block copolymer p85
keywords: Odontogenic differentiation; pluronics; pulp regeneration; stem cells; tooth germs