title: Optimization of nutrition factors on chitinase production from a newly isolated Chitiolyticbacter meiyuanensis SYBC-H1.
abstract: The present study reports statistical medial optimization for chitinase production by a novel bacterial strain isolated from soil recently, which the name Chitinolyticbacter meiyuanensis SYBC-H1 is proposed. A sequential statistical methodology comprising of Plackett-Burman and response surface methodology (RSM) was applied to enhance the fermentative production of chitinase, in which inulin was firstly used as an effective carbon source. As a result, maximum chitinase activity of 5.17 U/mL was obtained in the optimized medium, which was 15.5-fold higher than that in the basal medium. The triplicate verification experiments were performed under the optimized nutrients levels which indicated that it well agreed with the predicted value.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi 214122 , People's Republic of China ; Fishery Extension Station of Yingzhou District , 28 Yingzhou Road, Fuyang 236000, Anhui Province , People's Republic of China.
authors.forename: Zhikui
authors.initials: Z
authors.lastname: Hao
authors.forename: Yujie
authors.initials: Y
authors.lastname: Cai
authors.forename: Xiangru
authors.initials: X
authors.lastname: Liao
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhiyou
authors.initials: Z
authors.lastname: Fang
authors.forename: Dabing
authors.initials: D
authors.lastname: Zhang
keywords: chitinase; inulin; medium optimization; meiyuanensis SYBC-H1; response surface method
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