title: Haemoproteus syrnii in Strix aluco from France: morphology, stages of sporogony in a hippoboscid fly, molecular characterization and discussion on the identification of Haemoproteus species.
abstract: In France, Haemoproteus syrnii is frequently found in the Tawny Owl, Strix aluco. Additional and complementary features of this species, and in particular the characteristics of volutin, are presented. The authors consider the volutin granules as constant in a given species, and discuss their taxonomic value. These cytoplasmic inclusions appear early during the first stages of development of the gametocytes as an initial granule which multiplies as the parasite develops. They were reported in some species of Haemoproteus but are seldom considered as a specific character and described with precision. Sporogony from ookinete to apparently mature sporozoites appears to take place in a pupiparous hippoboscid (Ornithomyia sp.). One specimen was crushed between two slides and stained with Giemsa. Gametocytes of H. syrnii, many ookinetes, an immature oocyst and mature sporozoites were observed spread all over the smear. This would allow classifying this species in the Haemoproteus subgenus. We provide associated molecular data derived from the cyt b and cox 1 gene from this parasite. We discuss the problems of multiple infections and the difficulties in identifying Haemoproteus species and in deriving conclusions from sequences deposited in databases.
authors.affiliation: UMR 7245 MCAM MNHN CNRS, Muséum National d'Histoire Naturelle, 61 rue Buffon, CP 52, 75231 Paris Cedex 05, France.
authors.forename: Grégory
authors.initials: G
authors.lastname: Karadjian
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Puech
authors.forename: Linda
authors.initials: L
authors.lastname: Duval
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Chavatte
authors.forename: Georges
authors.initials: G
authors.lastname: Snounou
authors.forename: Irène
authors.initials: I
authors.lastname: Landau
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000453:epidemiology / Q000469:parasitology*; D002467:Cell Nucleus / Q000648:ultrastructure; D045303:Cytochromes b / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D004912:Erythrocytes / Q000469:parasitology / Q000648:ultrastructure; D005260:Female; D005602:France / Q000453:epidemiology; D016787:Haemosporida / Q000145:classification* / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D011529:Protozoan Infections, Animal / Q000453:epidemiology / Q000469:parasitology*; D033761:Spores, Protozoan / Q000502:physiology; D020305:Strigiformes / Q000469:parasitology*
chemical_list: D045303:Cytochromes b; D003576:Electron Transport Complex IV
references.citation: Biochem J. 1999 Jun 15;340 ( Pt 3):759-66
references.pmid: 10359662
references.citation: Mol Ecol. 2007 Mar;16(6):1281-90
references.pmid: 17391413
references.citation: J Parasitol. 2008 Jun;94(3):709-15
references.pmid: 18605786
references.citation: FEBS Lett. 1999 Oct 29;460(2):217-20
references.pmid: 10544238
references.citation: Trends Parasitol. 2008 Jun;24(6):247-8
references.pmid: 18440865
references.citation: J Egypt Soc Parasitol. 1998 Apr;28(1):221-8
references.pmid: 9617059
references.citation: J Parasitol. 2012 Apr;98(2):388-97
references.pmid: 21992108
references.citation: Biochem J. 1998 Apr 15;331 ( Pt 2):583-9
references.pmid: 9531501
references.citation: Parasitology. 2006 Sep;133(Pt 3):279-88
references.pmid: 16740182
references.citation: Zootaxa. 2013 Feb 18;3616:85-94
references.pmid: 24758794
references.citation: J Parasitol. 2013 Jun;99(3):513-21
references.pmid: 23240808
references.citation: J Parasitol. 2010 Aug;96(4):783-92
references.pmid: 20486741
references.citation: Malar J. 2007 Nov 29;6:157
references.pmid: 18045505
references.citation: J Biol Chem. 2001 Jul 13;276(28):26114-21
references.pmid: 11371561
references.citation: Parasitol Res. 2013 Jun;112(6):2159-69
references.pmid: 23504040
references.citation: J Parasitol. 1996 Jun;82(3):489-91
references.pmid: 8636858
references.citation: Can J Zool. 1965 Nov;43(6):927-32
references.pmid: 4953820
references.citation: Biochem J. 2000 Aug 1;349 Pt 3:737-45
references.pmid: 10903134
references.citation: Biochem J. 2000 Apr 1;347 Pt 1:243-53
references.pmid: 10727425
references.citation: Int J Parasitol. 2011 Aug 15;41(10):1019-27
references.pmid: 21683082
references.citation: Mol Phylogenet Evol. 2008 Apr;47(1):261-73
references.pmid: 18248741
references.citation: Syst Biol. 2010 May;59(3):307-21
references.pmid: 20525638