title: A new approach for the study of lung smooth muscle phenotypes and its application in a murine model of allergic airway inflammation.
abstract: Phenotypes of lung smooth muscle cells in health and disease are poorly characterized. This is due, in part, to a lack of methodologies that allow for the independent and direct isolation of bronchial smooth muscle cells (BSMCs) and vascular smooth muscle cells (VSMCs) from the lung. In this paper, we describe the development of a bi-fluorescent mouse that permits purification of these two cell populations by cell sorting. By subjecting this mouse to an acute allergen based-model of airway inflammation that exhibits many features of asthma, we utilized this tool to characterize the phenotype of so-called asthmatic BSMCs. First, we examined the biophysical properties of single BSMCs from allergen sensitized mice and found increases in basal tone and cell size that were sustained ex vivo. We then generated for the first time, a comprehensive characterization of the global gene expression changes in BSMCs isolated from the bi-fluorescent mice with allergic airway inflammation. Using statistical methods and pathway analysis, we identified a number of differentially expressed mRNAs in BSMCs from allergen sensitized mice that code for key candidate proteins underlying changes in matrix formation, contractility, and immune responses. Ultimately, this tool will provide direction and guidance for the logical development of new markers and approaches for studying human lung smooth muscle.
authors.affiliation: The Pulmonary Center, Boston University School of Medicine, Boston, Massachusetts, United States of America.
authors.forename: Jesus
authors.initials: J
authors.lastname: Paez-Cortez
authors.forename: Ramaswamy
authors.initials: R
authors.lastname: Krishnan
authors.forename: Anneliese
authors.initials: A
authors.lastname: Arno
authors.forename: Linh
authors.initials: L
authors.lastname: Aven
authors.forename: Sumati
authors.initials: S
authors.lastname: Ram-Mohan
authors.forename: Kruti R
authors.initials: KR
authors.lastname: Patel
authors.forename: Jining
authors.initials: J
authors.lastname: Lu
authors.forename: Oliver D
authors.initials: OD
authors.lastname: King
authors.forename: Xingbin
authors.initials: X
authors.lastname: Ai
authors.forename: Alan
authors.initials: A
authors.lastname: Fine
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D001249:Asthma / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D001980:Bronchi / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D016535:Bronchial Hyperreactivity / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D048429:Cell Size; D004195:Disease Models, Animal; D005453:Fluorescence; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D007114:Immunization; D007249:Inflammation / Q000235:genetics / Q000276:immunology / Q000473:pathology; D051379:Mice; D008822:Mice, Transgenic; D009131:Muscle, Smooth, Vascular / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D032389:Myocytes, Smooth Muscle / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D010047:Ovalbumin / Q000276:immunology; D010641:Phenotype; D020543:Proteome / Q000235:genetics / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics / Q000276:immunology; D059010:Single-Cell Analysis
chemical_list: D000485:Allergens; D020543:Proteome; D012333:RNA, Messenger; D010047:Ovalbumin
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