title: [Diagnostic value of BRAFV600E and RET/PTC oncogenes in thyroid nodule aspirates].
abstract: Fine-needle aspiration cytology (FNC) is the primary means to distinguish benign form malignant nodules. Aim of this study is to evaluate the diagnostic value of BRAF(V600E) and RET/PTC oncogenes in a large cohort of thyroid nodules with inconclusive FNC. We searched for BRAF(V600E) and RET/PTC in 299 thyroid nodule aspirates then removed by surgery. RET/PTC demonstrated a poor specificity. The search for BRAF(V600E) demonstrated to be useful in 25 cases, identifying a PTC in 2 false negative, 2 inadequate, 11 indeterminate and 10 suspicious FNC. Detection of BRAF(V600E) revealed to be a useful tool to refine inconclusive cytology.
authors.forename: Anna
authors.initials: A
authors.lastname: Guerra
authors.forename: Mario
authors.initials: M
authors.lastname: Carrano
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Angrisani
authors.forename: Mario
authors.initials: M
authors.lastname: Vitale
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000737:chemistry / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology / Q000601:surgery; D014408:Biomarkers, Tumor / Q000032:analysis*; D044963:Biopsy, Fine-Needle; D002291:Carcinoma, Papillary / Q000737:chemistry / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology / Q000601:surgery; D004273:DNA, Neoplasm / Q000235:genetics; D003937:Diagnosis, Differential; D006801:Humans; D020125:Mutation, Missense; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D009857:Oncogenes; D017354:Point Mutation; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D013959:Thyroid Diseases / Q000175:diagnosis / Q000235:genetics / Q000473:pathology / Q000601:surgery; D013964:Thyroid Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology / Q000601:surgery; D016606:Thyroid Nodule / Q000737:chemistry / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D013965:Thyroidectomy
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D015514:Oncogene Proteins, Fusion; D011505:Protein-Tyrosine Kinases; C093573:ret-PTC fusion oncoproteins, human; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf