title: Accelerated calcium phosphate cement degradation due to incorporation of glucono-delta-lactone microparticles.
abstract: Injectable calcium phosphate cements (CPC) are frequently used for filling of bone defects due to their excellent osteocompatibility. Their poor degradability, however, limits complete regeneration of bone defects. Organic additives that produce acid by-products are particularly attractive to create macroporosity in situ since CPC degrade by acid dissolution. The aim of the current study was to investigate whether glucono-delta-lactone (GDL) can be used as acid-producing microparticles for incorporation into CPC without compromising its osteocompatibility. Characterization studies confirmed that CPCs containing either low or high amounts of GDL were injectable and self-setting, while a considerable amount of porosity was formed already within 1 day of incubation in phosphate buffered saline due to dissolution of GDL. Histomorphometrical evaluation after 2 weeks of implantation revealed that CPC containing 10% of GDL degraded faster and was replaced by more bone tissue than CPCs containing either Poly (lactic-co-glycolic acid) (PLGA) or gelatin microspheres. Summarizing, the current study showed that CPCs containing appropriate amounts of GDL display accelerated degradation and new bone formation compared with CPCs containing microparticles made of conventional polymers such as PLGA or gelatin.
authors.affiliation: Department of Biomaterials, Radboud University Nijmegen Medical Centre , Nijmegen, The Netherlands .
authors.forename: Rosa P
authors.initials: RP
authors.lastname: Félix Lanao
authors.forename: Kemal
authors.initials: K
authors.lastname: Sariibrahimoglu
authors.forename: Huanan
authors.initials: H
authors.lastname: Wang
authors.forename: Joop G C
authors.initials: JG
authors.lastname: Wolke
authors.forename: John A
authors.initials: JA
authors.lastname: Jansen
authors.forename: Sander C G
authors.initials: SC
authors.lastname: Leeuwenburgh
mesh_terms: D000818:Animals; D001843:Bone Cements / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D002417:Cattle; D005260:Female; D005942:Gluconates / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D007783:Lactones / Q000737:chemistry*; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010012:Osteogenesis / Q000187:drug effects; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D019919:Prosthesis Implantation; D011817:Rabbits; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D001843:Bone Cements; D002130:Calcium Phosphates; D005942:Gluconates; D007783:Lactones; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C020243:calcium phosphate; C010730:beta-glucono-1,5-lactone