title: Human umbilical cord-derived MSC culture: the replacement of animal sera with human cord blood plasma.
abstract: Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) hold great potential for their therapeutic use in various clinical diseases. Many publications have reported on human blood-derived alternatives to animal serum for culturing mesenchymal stem cells, such as human serum, allogenic umbilical cord blood serum, and human platelet derivatives. However, it is not clear whether human umbilical cord blood plasma (UCBP), as the surplusage of umbilical cord blood mesenchymal stem cell extraction, could be used. In this study, in order to make the best of umbilical cord blood, the human UCBP was dialyzed to replace fetal bovine serum (FBS) in the culture medium. hUC-MSCs were cultured in the new medium. Cell growth rate, specific biomarkers, and differentiation properties were detected to characterize the cell proliferation and MSC-specific properties. The hUC-MSCs cultured in such derived medium were verified with proliferation rate, cluster differentiation markers, cell cycle, as well as differentiation capabilities. Such dialyzed human UCBP is fully comparable with, if not superior to, FBS in deriving and culturing hUC-MSCs.
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology, Beijing, 100124, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ding
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
authors.forename: Jing Bo
authors.initials: JB
authors.lastname: Feng
authors.forename: Ying
authors.initials: Y
authors.lastname: Qiu
authors.forename: Dong Sheng
authors.initials: DS
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D017931:DNA Primers / Q000235:genetics; D005312:Fetal Blood; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D010949:Plasma / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D017931:DNA Primers
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