title: Dissecting the oligonucleotide binding properties of a disordered chaperone protein using surface plasmon resonance.
abstract: We have used surface plasmon resonance to investigate the nucleic acid binding properties of the core protein of hepatitis C virus, a disordered protein believed to chaperone the genomic RNA. It was previously shown that a peptide (peptide E) corresponding to the association of two basic clusters of core enhances the annealing and the dimerization of nucleic acid fragments derived from a stem loop (SL2) in the 3' untranslated region of the hepatitis C virus genome. However, strong aggregation of nucleic acids by core or peptide E in the excess of the latter precluded the characterization of their binding parameters up to now. By careful design of surface plasmon resonance experiments, we obtained accurate binding parameters for the interaction of peptide E with SL2-derived oligonucleotides of different lengths and sequences, in form of stem-loop, duplex or strand. Peptide E was found to bind in a salt dependent manner to all oligonucleotides assayed. Affinity data identify at least two binding modes, of which one is independent of sequence/structure, and the other is specific to the SL2 stem-loop fold. Stoichiometry data support a multi-motif binding model allowing formation of higher-order complexes. We propose that the modular binding mode demonstrated for structured RNA-binding proteins also applies to this disordered chaperone and is relevant to its activity.
authors.affiliation: Biotechnologie et signalisation cellulaire, Université de Strasbourg, CNRS, BP10413, 67412 Illkirch, France and Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Faculté de Pharmacie, Université de Strasbourg, 67401, Illkirch, France.
authors.forename: Mireille
authors.initials: M
authors.lastname: Baltzinger
authors.forename: Kamal Kant
authors.initials: KK
authors.lastname: Sharma
authors.forename: Yves
authors.initials: Y
authors.lastname: Mély
authors.forename: Danièle
authors.initials: D
authors.lastname: Altschuh
mesh_terms: D064267:Intrinsically Disordered Proteins / Q000737:chemistry / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D009843:Oligoribonucleotides / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D014758:Viral Core Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D064267:Intrinsically Disordered Proteins; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D010455:Peptides; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins; C069130:nucleocapsid protein, Hepatitis C virus
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