title: [Reverse genetics platform construction of influenza pandemic virus strain].
abstract: OBJECTIVE
  Reverse genetics was used to construct the platform of flu pandemic strain A/California/07/2009 (H1N1).
  
  
  METHODS
  Eight genes fragements were amplified and ligated with bidirectional vector, recombinant plasmids were co transfected to the 293 T cells and rescued the virus. Gene sequencing, antigenic analysis and growth property were used to evaluate the rescued virus.
  
  
  RESULTS
  Rescued virus show the genes sequence correct, keep the same antigenicity and similar growth property compared with wild type virus.
  
  
  CONCLUSIONS
  The pandemic virus reverse genetics platform of A/California/07/2009 (H1N1) were built. Based on this platform, rescued virus hold the similarity of antigenicity and growth ability with wild type virus.
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Bo
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wei-Juan
authors.initials: WJ
authors.lastname: Huang
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhao
authors.forename: Jun-Feng
authors.initials: JF
authors.lastname: Guo
authors.forename: Da-Yan
authors.initials: DY
authors.lastname: Wang
authors.forename: Yue-Long
authors.initials: YL
authors.lastname: Shu
mesh_terms: D009975:Orthomyxoviridae / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D058873:Pandemics; D010957:Plasmids; D059386:Reverse Genetics