title: Increased longevity mediated by yeast NDI1 expression in Drosophila intestinal stem and progenitor cells.
abstract: A functional decline in tissue stem cells and mitochondrial dysfunction have each been linked to aging and multiple aging-associated pathologies. However, the interplay between energy homeostasis, stem cells, and organismal aging remains poorly understood. Here, we report that expression of the single-subunit yeast alternative NADH dehydrogenase, ndi1, in Drosophila intestinal stem and progenitor cells delays the onset of multiple markers of intestinal aging and extends lifespan. In addition, expression of ndi1 in the intestine increases feeding behavior and results in organismal weight gain. Consistent with increased nutrient uptake, flies expressing ndi1 in the digestive tract display a systemic reduction in the activity of AMP-activated protein kinase (AMPK), a key cellular energy sensor. Together, these results demonstrate that ndi1 expression in the intestinal epithelium is an effective strategy to delay tissue and organismal aging.
authors.affiliation: Department of Integrative Biology and Physiology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Jae H
authors.initials: JH
authors.lastname: Hur
authors.forename: Sepehr
authors.initials: S
authors.lastname: Bahadorani
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Graniel
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Koehler
authors.forename: Matthew
authors.initials: M
authors.lastname: Ulgherait
authors.forename: Michael
authors.initials: M
authors.lastname: Rera
authors.forename: D Leanne
authors.initials: DL
authors.lastname: Jones
authors.forename: David W
authors.initials: DW
authors.lastname: Walker
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000201:enzymology; D000375:Aging / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000818:Animals; D030801:Animals, Genetically Modified; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D042967:Electron Transport Complex I / Q000235:genetics / Q000378:metabolism*; D005247:Feeding Behavior; D005260:Female; D015870:Gene Expression; D005800:Genes, Fungal; D007422:Intestines / Q000166:cytology / Q000201:enzymology; D008136:Longevity / Q000235:genetics / Q000502:physiology*; D008297:Male; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: C493237:Ndi1 protein, S cerevisiae; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D042967:Electron Transport Complex I
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