title: Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance.
abstract: Non-tuberculous mycobacteria isolated at the Central Public Health Laboratory from Mato Grosso do Sul in 2003 and 2004 were identified by conventional phenotypic methods (TI) and by PCR-Restriction Enzyme Analysis (PRA) using the hsp65 gene as target (PRA-hsp65). With 15 of the 32 analysed isolates, results of both methods were concordant, being 8 Mycobacterium avium, 3 M. fortutium, 1 M. kansasii, 1 M. flavescens, 1 M. peregrinum and 1 Nocardia brasiliensis. TI of 12 isolates was inconclusive. Novel PRA-hsp65 patterns were observed with 11 isolates. Medical data were evaluated for inference of clinical relevance of these isolates.
authors.affiliation: Laboratório Central de Saúde Pública de Mato Grosso do Sul , Mato Grosso do Sul, MS , Brasil.
authors.forename: Paulo Ricardo
authors.initials: PR
authors.lastname: de Souza Moraes
authors.forename: Erica
authors.initials: E
authors.lastname: Chimara
authors.forename: Maria Alice da Silva
authors.initials: MA
authors.lastname: Telles
authors.forename: Suely Yoko Misuka
authors.initials: SY
authors.lastname: Ueki
authors.forename: Eunice Atsuko Totumi
authors.initials: EA
authors.lastname: Cunha
authors.forename: Michael Robin
authors.initials: MR
authors.lastname: Honer
authors.forename: Sylvia Cardoso
authors.initials: SC
authors.lastname: Leão
keywords: Identification; Mycobacterium; PCR
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