title: Identification and characterisation of microRNAs in young adults of Angiostrongylus cantonensis via a deep-sequencing approach.
abstract: Angiostrongylus cantonensis is an important causative agent of eosinophilic meningitis and eosinophilic meningoencephalitis in humans. MicroRNAs (miRNAs) are small non-coding RNAs that participate in a wide range of biological processes. This study employed a deep-sequencing approach to study miRNAs from young adults of A. cantonensis. Based on 16,880,456 high-quality reads, 252 conserved mature miRNAs including 10 antisense miRNAs that belonging to 90 families, together with 10 antisense miRNAs were identified and characterised. Among these sequences, 53 miRNAs from 25 families displayed 50 or more reads. The conserved miRNA families were divided into four groups according to their phylogenetic distribution and a total of nine families without any members showing homology to other nematodes or adult worms were identified. Stem-loop real-time polymerase chain reaction analysis of aca-miR-1-1 and aca-miR-71-1 demonstrated that their level of expression increased dramatically from infective larvae to young adults and then decreased in adult worms, with the male worms exhibiting significantly higher levels of expression than female worms. These findings provide information related to the regulation of gene expression during the growth, development and pathogenesis of young adults of A. cantonensis.
authors.affiliation: Institute of Biomedical Sciences, College of Medicine, Chang Gung University, TaoyuanCounty, Taiwan.
authors.forename: Shih-Hsin
authors.initials: SH
authors.lastname: Chang
authors.forename: Petrus
authors.initials: P
authors.lastname: Tang
authors.forename: Cheng-Hung
authors.initials: CH
authors.lastname: Lai
authors.forename: Ming-Ling
authors.initials: ML
authors.lastname: Kuo
authors.forename: Lian-Chen
authors.initials: LC
authors.lastname: Wang
mesh_terms: D017185:Angiostrongylus cantonensis / Q000235:genetics* / Q000254:growth & development; D000818:Animals; D005260:Female; D015870:Gene Expression / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D008018:Life Cycle Stages / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000302:isolation & purification*; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D017423:Sequence Analysis, RNA / Q000379:methods*; D017206:Strongylida Infections / Q000235:genetics*
chemical_list: D035683:MicroRNAs
references.citation: Nat Rev Genet. 2012 Apr 18;13(5):358-69
references.pmid: 22510765
references.citation: Nat Genet. 2005 Jul;37(7):766-70
references.pmid: 15965474
references.citation: Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2009 Jun;27(3):248-50
references.pmid: 19852370
references.citation: BMC Mol Biol. 2009 Oct 25;10:97
references.pmid: 19852860
references.citation: Cell. 2009 Jan 23;136(2):215-33
references.pmid: 19167326
references.citation: Mol Biochem Parasitol. 2011 Sep;179(1):47-50
references.pmid: 21624399
references.citation: BMC Genomics. 2012 Jan 04;13:4
references.pmid: 22216965
references.citation: PLoS One. 2008;3(12):e4034
references.pmid: 19107204
references.citation: Parasitol Res. 2010 Sep;107(4):915-22
references.pmid: 20567985
references.citation: Science. 2005 Jul 8;309(5732):310-1
references.pmid: 15919954
references.citation: Am J Trop Med Hyg. 2004 Aug;71(2):222-6
references.pmid: 15306715
references.citation: Exp Parasitol. 2011 Jun;128(2):116-20
references.pmid: 21356210
references.citation: Am J Trop Med Hyg. 2011 Oct;85(4):685-90
references.pmid: 21976573
references.citation: Nan Fang Yi Ke Da Xue Xue Bao. 2007 May;27(5):584-7
references.pmid: 17545061
references.citation: Methods Mol Biol. 2011;721:43-66
references.pmid: 21431678
references.citation: Nucleic Acids Res. 2010 Jul;38(Web Server issue):W385-91
references.pmid: 20478825
references.citation: Nat Genet. 2006 Jun;38 Suppl:S2-7
references.pmid: 16736019
references.citation: Nature. 2000 Feb 24;403(6772):901-6
references.pmid: 10706289
references.citation: Methods Mol Biol. 2012;822:183-8
references.pmid: 22144199
references.citation: J Biol Chem. 2011 Jan 28;286(4):2550-8
references.pmid: 20966081
references.citation: Emerg Infect Dis. 2011 Jul;17(7):1335-6
references.pmid: 21762614
references.citation: Nature. 2010 Feb 25;463(7284):1084-8
references.pmid: 20118916
references.citation: Aging Cell. 2006 Jun;5(3):235-46
references.pmid: 16842496
references.citation: Acta Trop. 2010 Mar;113(3):289-94
references.pmid: 19945419
references.citation: Mol Biochem Parasitol. 2010 Feb;169(2):87-94
references.pmid: 19874857
references.citation: Mol Cell. 2010 Jun 25;38(6):803-14
references.pmid: 20417140
references.citation: Infection. 2004 Apr;32(2):116-8
references.pmid: 15057577
references.citation: Circ Res. 2011 Jan 21;108(2):219-34
references.pmid: 21252150
references.citation: Cell. 2008 May 30;133(5):903-15
references.pmid: 18510933
references.citation: PLoS Negl Trop Dis. 2009;3(2):e368
references.pmid: 19190771
references.citation: Int J Parasitol. 1989 Sep;19(6):617-20
references.pmid: 2807717
references.citation: Curr Opin Genet Dev. 2011 Aug;21(4):511-7
references.pmid: 21530229
references.citation: Parasitol Res. 2012 Jun;110(6):2501-7
references.pmid: 22218922
references.citation: Lancet Infect Dis. 2008 Oct;8(10):621-30
references.pmid: 18922484
references.citation: BMC Genomics. 2010 Jan 21;11:55
references.pmid: 20092619
references.citation: Adv Parasitol. 1965;3:223-48
references.pmid: 5334821
references.citation: Am J Trop Med Hyg. 1975 Nov;24(6 Pt 1):921-31
references.pmid: 1200257
references.citation: Parasitol Res. 2011 Aug;109(2):369-78
references.pmid: 21344211
references.citation: Cell. 1993 Dec 3;75(5):843-54
references.pmid: 8252621
references.citation: Am J Epidemiol. 1966 Jul;84(1):120-31
references.pmid: 5944728
references.citation: Clin Infect Dis. 2009 Feb 1;48(3):322-7
references.pmid: 19123863
references.citation: Parasitol Res. 2010 Apr;106(5):1027-32
references.pmid: 20177908
references.citation: Travel Med Infect Dis. 2008 Jan-Mar;6(1-2):41-4
references.pmid: 18342273
references.citation: Nat Rev Genet. 2010 Sep;11(9):597-610
references.pmid: 20661255
references.citation: Intern Med. 2011;50(7):771-4
references.pmid: 21467715
references.citation: Jpn J Infect Dis. 2004 Aug;57(4):184-6
references.pmid: 15329455
references.citation: Nucleic Acids Res. 2005 Nov 27;33(20):e179
references.pmid: 16314309
references.citation: Plant Mol Biol. 2011 Sep;77(1-2):47-58
references.pmid: 21607657
references.citation: Soc Sci Med. 1992 Jan;34(2):199-212
references.pmid: 1738873
references.citation: Travel Med Infect Dis. 2008 Sep;6(5):301-4
references.pmid: 18760253
references.citation: Nature. 2005 Jul 14;436(7048):214-20
references.pmid: 15951802
references.citation: Nature. 2004 Sep 16;431(7006):350-5
references.pmid: 15372042
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: Evol Dev. 2003 Jul-Aug;5(4):372-8
references.pmid: 12823453
references.citation: Parasitol Res. 2012 Jun;110(6):2413-22
references.pmid: 22215189
references.citation: Genomics. 2011 Aug;98(2):96-111
references.pmid: 21640815
references.citation: J Travel Med. 2009 Jul-Aug;16(4):292-4
references.pmid: 19674273
references.citation: BMC Genomics. 2010 Sep 28;11:521
references.pmid: 20920166
references.citation: Trends Genet. 2007 May;23(5):243-9
references.pmid: 17368621
references.citation: Parasitol Res. 2011 Sep;109(3):553-8
references.pmid: 21327987
references.citation: Nucleic Acids Res. 2011 Jan;39(Database issue):D152-7
references.pmid: 21037258
references.citation: Genes Dev. 2005 Oct 1;19(19):2343-54
references.pmid: 16166373
references.citation: J Parasitol. 1968 Jun;54(3):432-6
references.pmid: 5757723
references.citation: Exp Parasitol. 2012 Jun;131(2):210-4
references.pmid: 22542800
references.citation: Am J Epidemiol. 1967 May;85(3):395-402
references.pmid: 6025084
references.citation: Parasitol Res. 2012 Jun;110(6):2145-53
references.pmid: 22146998
references.citation: PLoS Genet. 2011 Sep;7(9):e1002306
references.pmid: 21980307