title: Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells.
abstract: Transient gene expression (TGE) from mammalian cells is an increasingly important tool for the rapid production of recombinant proteins for research applications in biochemistry, structural biology, and biomedicine. Here we review methods for the transfection of human embryo kidney (HEK-293) and Chinese hamster ovary (CHO) cells in suspension culture using the cationic polymer polyethylenimine (PEI) for gene delivery.
authors.affiliation: Protein Expression Core Facility, EPFL, CH-1015 Lausanne, Switzerland; Laboratory of Cellular Biotechnology, EPFL, CH-1015 Lausanne, Switzerland. Electronic address: david.hacker@epfl.ch.
authors.forename: David L
authors.initials: DL
authors.lastname: Hacker
authors.forename: Divor
authors.initials: D
authors.lastname: Kiseljak
authors.forename: Yashas
authors.initials: Y
authors.lastname: Rajendra
authors.forename: Sarah
authors.initials: S
authors.lastname: Thurnheer
authors.forename: Lucia
authors.initials: L
authors.lastname: Baldi
authors.forename: Florian M
authors.initials: FM
authors.lastname: Wurm
mesh_terms: D000818:Animals; D016466:CHO Cells; D018929:Cell Culture Techniques / Q000295:instrumentation; D006224:Cricetinae; D003412:Cricetulus; D004337:Drug Carriers / Q000378:metabolism*; D004867:Equipment Design; D005822:Genetic Vectors / Q000008:administration & dosage*; D057809:HEK293 Cells; D006801:Humans; D011094:Polyethyleneimine / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics; D014162:Transfection / Q000379:methods*; D014780:Viruses / Q000235:genetics
chemical_list: D004337:Drug Carriers; D011994:Recombinant Proteins; D011094:Polyethyleneimine
keywords: CHO-DG44; HEK-293; Mammalian cells; Orbital shaking; Polyethylenimine; Recombinant protein; Suspension culture; Transfection; Transient gene expression
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