title: Wild type and mutants of the HET-s(218-289) prion show different flexibility at fibrillar ends: a simulation study.
abstract: The C-terminal segment (residues 218-289) of the HET-s protein of the filamentous fungus Podosporina anserina is a prion-forming domain. The structural model of the HET-s(218-289) amyloid fibril based on solid-state nuclear magnetic resonance (NMR) restraints shows a β solenoid topology which is comprised of a β-sheet core and interconnecting loops. For the single-point mutants Phe286Ala and Trp287Ala, slower aggregation rates in vitro and loss of prionic infectivity have been reported recently. Here we have used molecular dynamics to compare the flexibility of the mutants and wild type. The simulations, initiated from a trimeric aggregate extracted from the NMR structural model, show structural stability on a 100-ns time scale for wild type and mutants. Analysis of the fluctuations along the simulations reveals that the mutants are less flexible than the wild type in the C-terminal segment at only one of the two external monomers. Analysis of interaction energy and buried accessible surface indicates that residue Phe286 in particular is stabilized in the Trp287Ala mutant. The simulation results provide an atomistic explanation of the suggestion (based on indirect experimental evidence) that flexibility at the protofibril end(s) is required for fibril elongation. Moreover, they provide further evidence that the growth of the HET-s amyloid fibril is directional.
authors.affiliation: Department of Chemistry and Biomedical Sciences, Linnaeus University, 391 82, Kalmar, Sweden; Linnaeus University, Center for Biomaterials Chemistry, 391 82, Kalmar, Sweden; Department of Biochemistry, University of Zürich, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland.
authors.forename: Ran
authors.initials: R
authors.lastname: Friedman
authors.forename: Amedeo
authors.initials: A
authors.lastname: Caflisch
mesh_terms: D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D056004:Molecular Dynamics Simulation; D009154:Mutation / Q000235:genetics*; D018583:Pliability; D011487:Protein Conformation; D055503:Protein Multimerization
chemical_list: D005656:Fungal Proteins; C083891:HET-S protein, Podospora anserina
keywords: aggregation; amyloid; fibril growth; molecular dynamics