title: The inhibitory effects of phenolic and terpenoid compounds from Baccharis trimera in Siha cells: differences in their activity and mechanism of action.
abstract: Baccharis trimera is used in folk medicine as a tea for digestive and liver diseases. It possesses anti-inflammatory and antioxidant properties that are related to the presence of phenolic compounds. The aim of this work was to investigate the anti-proliferative properties of phenolic (PHE) and terpenoid (SAP) compounds from B. trimera on human cervical cancer. The treatment of SiHa cells with PHE for 24 h suppressed colony formation in a dose-dependent manner, inhibited proliferation and inhibited cell motility. Although SAP inhibited the proliferation of SiHa cells in a dose-dependent manner, it increased colony formation and did not inhibit cell motility. PHE and SAP also promoted a significant increase in lactate dehydrogenase levels in the culture medium in a dose-dependent manner, indicating a loss of cell membrane integrity. Moreover, PHE promoted necrotic cell death, whereas SAP induced apoptosis. These compounds are new anticancer prototypes due their significant anticancer activity demonstrated herein.
authors.affiliation: Laboratório de Fitoquímica e Síntese Orgânica (LAFIS), Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90610-000, RS, Brazil. cristiane_gabi@yahoo.com.br
authors.forename: Cristiane B
authors.initials: CB
authors.lastname: de Oliveira
authors.forename: Lucimara N
authors.initials: LN
authors.lastname: Comunello
authors.forename: Erica S
authors.initials: ES
authors.lastname: Maciel
authors.forename: Scheron R
authors.initials: SR
authors.lastname: Giubel
authors.forename: Alessandra N
authors.initials: AN
authors.lastname: Bruno
authors.forename: Eduardo C F
authors.initials: EC
authors.lastname: Chiela
authors.forename: Guido
authors.initials: G
authors.lastname: Lenz
authors.forename: Simone C B
authors.initials: SC
authors.lastname: Gnoatto
authors.forename: Andréia
authors.initials: A
authors.lastname: Buffon
authors.forename: Grace
authors.initials: G
authors.lastname: Gosmann
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis; D031175:Baccharis / Q000737:chemistry*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020128:Inhibitory Concentration 50; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009336:Necrosis; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D013729:Terpenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010636:Phenols; D010936:Plant Extracts; D013729:Terpenes; D007770:L-Lactate Dehydrogenase
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