title: In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil.
abstract: An Ehrlichia canis isolate was obtained from an naturally infected dog exhibiting clinical signs of ehrlichiosis in São Paulo Municipality, state of São Paulo, Brazil. The isolate was characterized by PCR and DNA sequencing of portions of the ehrlichial genes dsb, 16SrRNA, and p28. Partial dsb and 16S rRNA sequences were identical to three and five other E. canis strains, respectively, from different countries and continents (including North America, Africa, Asia and Europe). Conversely, the p28 partial sequence for this E. canis (São Paulo) differed by 1, 2, and 2 nucleotides from the corresponding sequences of the E. canis strains Jake (from USA), Oklahoma (USA), and VHE (Venezuela), respectively. The results in this study indicate that E. canis is the only recognized Ehrlichia species infecting dogs in Brazil.
authors.affiliation: Departamento de Clínica Médica Veterinária, Faculdade de Agronomia e Medicina Veterinária, Universidade Federal de Mato Grosso , Cuiabá, MT , Brasil ; Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo , São Paulo, SP , Brasil.
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Aguiar
authors.forename: Mitika K
authors.initials: MK
authors.lastname: Hagiwara
authors.forename: Marcelo B
authors.initials: MB
authors.lastname: Labruna
keywords: Dog; Ehrlichia canis; Ehrlichiosis; Molecular Characterization
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