title: The effect of vacuum packaging, EDTA, oregano and thyme oils on the microbiological quality of chicken's breast.
abstract: The effect of ethylenediaminetetraacetate (EDTA), oregano (Origanum vulgare) and thyme (Thymus vulgaris) oils, on the chicken breast fillets was examined in this study. The chicken breast fillets were stored under vacuum packaging (VP), at 4 ± 0.5 °C for a period of 18 days. There were used the following treatments of chicken breast fillets: Air-packaged (AC, control samples), vacuum-packaged (VPC, control samples), VP with EDTA solution 1.50% w/w (VPEC, control samples), VP with oregano oil 0.20% v/w (VP + O) and VP with thyme oil 0.20% v/w, (VP + T). The quality assessment for vacuum packaging of the product in accordance with the terms above and EDTA treatment, oregano and thyme oil was established by microbiological analyzes. The microbiological properties as the total viable counts on Plate Count Agar, after incubation for 2 days at 37 °C and coliform bacteria on Violet Red Bile Glucose agar incubated at 37 °C for 24 h, lactobacilli on Rogosa and Sharpe agar after incubation 48-78 h at 37 °C in an aerobic atmosphere supplemented with carbon dioxide (5% CO2) and Pseudomonas aeruginosa on Pseudomonas Isolation agar (PIA, Oxoid, UK) after incubation at 48 h at 35 °C were monitored. The using of oregano, thyme oil and EDTA with combination of vacuum packaging has significant effects to reduction of all followed groups of microorganisms compared with control group without vacuum packaging and untreated control group. The natural preservatives can be used as alternatives to chemical additives which could extend the meat and meat products shelf life. The knowledge about them can have an important economic feedback by reducing losses attributed to spoilage and by allowing the products to reach distant and new markets. This study shows how using of natural antimicrobials can extend the shelf-life of the meat product.
authors.affiliation: Department of Animal Products Evaluation and Processing, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic.
authors.forename: Adriana
authors.initials: A
authors.lastname: Pavelková
authors.affiliation: Department of Microbiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. Andreja Hlinku 2, 949 76 Nitra, Slovak Republic. Electronic address: miroslava.kacaniova@gmail.com.
authors.forename: Miroslava
authors.initials: M
authors.lastname: Kačániová
authors.affiliation: Department of Marketing, Faculty of Economy, Slovak University of Agriculture, Nitra, Slovak Republic.
authors.forename: Elena
authors.initials: E
authors.lastname: Horská
authors.affiliation: Department of Green's Biotechnics, Horticulture and Landscape Engineering Faculty, Slovak University of Agriculture, Nitra, Slovak Republic.
authors.forename: Katarína
authors.initials: K
authors.lastname: Rovná
authors.affiliation: Department of Microbiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. Andreja Hlinku 2, 949 76 Nitra, Slovak Republic.
authors.forename: Lukáš
authors.initials: L
authors.lastname: Hleba
authors.affiliation: Department of Microbiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. Andreja Hlinku 2, 949 76 Nitra, Slovak Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Petrová
mesh_terms: D000818:Animals; D058491:Bacterial Load; D001688:Biological Products / Q000302:isolation & purification / Q000494:pharmacology*; D002645:Chickens; D003257:Consumer Product Safety; D004492:Edetic Acid / Q000494:pharmacology; D018857:Food Packaging / Q000379:methods*; D005520:Food Preservatives / Q000302:isolation & purification / Q000494:pharmacology*; D007778:Lactobacillus / Q000187:drug effects / Q000254:growth & development; D008460:Meat / Q000382:microbiology*; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology*; D031341:Origanum / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D012034:Refrigeration; D046930:Thymus Plant / Q000737:chemistry; D014618:Vacuum
chemical_list: D001688:Biological Products; D005520:Food Preservatives; D009822:Oils, Volatile; D004492:Edetic Acid
keywords: Chicken meat; Essential oils; Microflora; Packaging