title: A chemical genomics approach to identification of interactions between bioactive molecules and alternative reading frame proteins.
abstract: Magic Tag® immobilisation of bioactive molecules coupled with bacteriophage display, followed by an ELISA assay, provides a protocol that can probe interactions of drugs with putative products of alternative initiation of translation as exemplified by the binding of immobilised flecainide to protein products of genes linked to sudden cardiac death.
authors.affiliation: Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK. p.c.taylor@warwick.ac.uk.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Taylor
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Clark
authors.forename: Andrew
authors.initials: A
authors.lastname: Marsh
authors.forename: Donald R J
authors.initials: DR
authors.lastname: Singer
authors.forename: Suzanne J
authors.initials: SJ
authors.lastname: Dilly
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000378:metabolism*; D017123:Bacteriophage T7 / Q000235:genetics; D001483:Base Sequence; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005424:Flecainide / Q000378:metabolism*; D023281:Genomics / Q000379:methods*; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis; D016364:Reading Frames
chemical_list: D000889:Anti-Arrhythmia Agents; D029721:Drosophila Proteins; D019151:Peptide Library; D010455:Peptides; D005424:Flecainide