title: [Molecular biological identification of fungal pathogens in FFPE tissue from cases of cephalic mycosis].
abstract: BACKGROUND
  Due to the lack of histopathological differentiation the unequivocal identification of fungal pathogens is rarely possible. In order to understand the pathogen spectrum causing cephalic mycosis the use of alternative methods is essential.
  
  
  METHODS
  In a retrospective study 24 formalin-fixed, paraffin-embedded (FFPE) samples from patients with histologically confirmed cerebral or cephalic mycosis were analyzed with molecular biological methods.
  
  
  RESULTS
  In two samples obtained during the patients' lifetime human as well as fungal DNA was detected, making an unambiguous diagnosis possible. For tissue that had been fixed over a longer period, detection of human and fungal DNA was possible merely in 60% and 47 % of the samples, respectively. Most frequently diagnosed were aspergillosis (n = 9), followed by mucormycosis (n = 2) and imported blastomycosis (n = 1).
  
  
  CONCLUSIONS
  Using biopsy material a DNA analysis seems promising although only with limited success using brain samples taken at autopsy which have been fixed over a longer period. For unambiguous retrospective diagnostics of pathogens when cephalic mycosis is suspected, the sample extraction for postmortem diagnostics should be performed prior to a long period of formalin fixation.
authors.affiliation: Abt. FG 16, Erreger von Pilz- und Parasiteninfektionen und Mykobakteriosen, Robert Koch-Institut, Nordufer 20, 13353, Berlin, Deutschland, BernhardtA@rki.de.
authors.forename: A
authors.initials: A
authors.lastname: Bernhardt
authors.forename: L
authors.initials: L
authors.lastname: de Boni
authors.forename: H A
authors.initials: HA
authors.lastname: Kretzschmar
authors.forename: K
authors.initials: K
authors.lastname: Tintelnot
mesh_terms: D000328:Adult; D000368:Aged; D001921:Brain / Q000382:microbiology / Q000473:pathology; D001927:Brain Diseases / Q000382:microbiology* / Q000473:pathology*; D020314:Central Nervous System Fungal Infections / Q000382:microbiology* / Q000473:pathology*; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D005260:Female; D005404:Fixatives; D005557:Formaldehyde; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D016533:Mycological Typing Techniques; D009894:Opportunistic Infections / Q000382:microbiology / Q000473:pathology; D016612:Paraffin Embedding; D010254:Paranasal Sinus Diseases / Q000382:microbiology / Q000473:pathology*
chemical_list: D004271:DNA, Fungal; D005404:Fixatives; D005557:Formaldehyde
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