title: Point of care creatinine measurement for diagnosis of renal disease using a disposable microchip.
abstract: A point-of-care device for the determination of elevated creatinine levels in blood is reported. This device potentially offers a new and simple clinical regime for the determination of creatinine that will give huge time savings and removal of several steps of determination. The test employs a disposable prefilled microchip and the handheld Medimate Multireader®. By optimizing the analytical conditions it was found that the LOD of the proposed method was 87 μM creatinine, close to the normal human serum levels that are in the range of 60 to 100 μM. A statistical analysis of the residual shows a normal distribution, indicating the absence of systematic errors in the proposed method. The test can be used to distinguish patients with renal insufficiency (creatinine levels >100 μM) from healthy persons. Long-term monitoring could furthermore distinguish between acute renal failure and chronic kidney disease by the rate of creatinine concentration rise.
authors.affiliation: Department of Analytical Chemistry and Food Technology, University of Castilla-La Mancha, Ciudad Real, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Ávila
authors.forename: Arjan
authors.initials: A
authors.lastname: Floris
authors.forename: Steven
authors.initials: S
authors.lastname: Staal
authors.forename: Ángel
authors.initials: Á
authors.lastname: Ríos
authors.forename: Jan
authors.initials: J
authors.lastname: Eijkel
authors.forename: Albert
authors.initials: A
authors.lastname: van den Berg
mesh_terms: D003404:Creatinine / Q000097:blood*; D004867:Equipment Design; D006801:Humans; D007674:Kidney Diseases / Q000097:blood* / Q000175:diagnosis; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D019095:Point-of-Care Systems
chemical_list: D003404:Creatinine
keywords: Creatinine; Kidney disease; Microchip; Point-of-care; Screening