title: Evaluation of antimicrobial activity of extracts of Tibouchina candolleana (melastomataceae), isolated compounds and semi-synthetic derivatives against endodontic bacteria.
abstract: This work describes the phytochemical study of the extracts from aerial parts of Tibouchina candolleana as well as the evaluation of the antimicrobial activity of extracts, isolated compounds, and semi-synthetic derivatives of ursolic acid against endodontic bacteria. HRGC analysis of the n-hexane extract of T. candolleana allowed identification of β-amyrin, α-amyrin, and β-sitosterol as major constituents. The triterpenes ursolic acid and oleanolic acid were isolated from the methylene chloride extract and identified. In addition, the flavonoids luteolin and genistein were isolated from the ethanol extract and identified. The antimicrobial activity was investigated via determination of the minimum inhibitory concentration (MIC) using the broth microdilution method. Amongst the isolated compounds, ursolic acid was the most effective against the selected endodontic bacteria. As for the semi-synthetic ursolic acid derivatives, only the methyl ester derivative potentiated the activity against Bacteroides fragilis.
authors.affiliation: Núcleo de Pesquisa em Ciências Exatas e Tecnológicas da Universidade de Franca , Franca, SP , Brasil.
authors.forename: Fernanda M
authors.initials: FM
authors.lastname: Dos Santos
authors.forename: Maria Gorete
authors.initials: MG
authors.lastname: de Souza
authors.forename: Antônio E Miller
authors.initials: AE
authors.lastname: Crotti
authors.forename: Carlos H G
authors.initials: CH
authors.lastname: Martins
authors.forename: Sérgio R
authors.initials: SR
authors.lastname: Ambrósio
authors.forename: Rodrigo C S
authors.initials: RC
authors.lastname: Veneziani
authors.forename: Márcio L Andrade
authors.initials: ML
authors.lastname: E Silva
authors.forename: Wilson R
authors.initials: WR
authors.lastname: Cunha
keywords: Tibouchina candolleana; antimicrobial activity; ursolic acid
references.citation: Planta Med. 2003 May;69(5):470-2
references.pmid: 12802734
references.citation: Methods. 2007 Aug;42(4):321-4
references.pmid: 17560319
references.citation: J Agric Food Chem. 2010 Oct 13;58(19):10404-9
references.pmid: 20809630
references.citation: Arch Oral Biol. 2005 Jul;50(7):645-51
references.pmid: 15892950
references.citation: Molecules. 2009 Jan 04;14(1):191-9
references.pmid: 19127247
references.citation: Chem Biodivers. 2010 Jul;7(7):1835-40
references.pmid: 20658673
references.citation: Fitoterapia. 2009 Oct;80(7):432-6
references.pmid: 19524643
references.citation: Oral Microbiol Immunol. 2004 Apr;19(2):71-6
references.pmid: 14871344
references.citation: Biomed Chromatogr. 2006 Aug;20(8):827-30
references.pmid: 16345013
references.citation: Curr Med Chem. 2004 Jun;11(11):1375-89
references.pmid: 15180572
references.citation: Chem Pharm Bull (Tokyo). 1999 Jun;47(6):824-7
references.pmid: 10507887
references.citation: Solid State Nucl Magn Reson. 1997 Dec;10(1-2):33-8
references.pmid: 9472789
references.citation: Phytother Res. 2006 Jun;20(6):474-8
references.pmid: 16619351
references.citation: Molecules. 2011 Jan 13;16(1):543-51
references.pmid: 21233793
references.citation: Int Endod J. 2007 Jan;40(1):2-10
references.pmid: 17209826
references.citation: J Ethnopharmacol. 1995 Dec 1;49(2):57-68
references.pmid: 8847885
references.citation: Planta Med. 2000 Jun;66(5):485-6
references.pmid: 10909277
references.citation: Phytochemistry. 1999 Dec;52(8):1661-6
references.pmid: 10647222
references.citation: Int Endod J. 2009 Apr;42(4):288-302
references.pmid: 19220510
references.citation: Z Naturforsch C J Biosci. 2008 Jul-Aug;63(7-8):497-502
references.pmid: 18810991
references.citation: Life Sci. 2005 Dec 22;78(5):431-41
references.pmid: 16198377
references.citation: J Chem Soc Perkin 1. 1976;(23):2475-83
references.pmid: 1034642
references.citation: Z Naturforsch C J Biosci. 2006 Jul-Aug;61(7-8):477-82
references.pmid: 16989305
references.citation: Int Endod J. 2009 Jul;42(7):555-67
references.pmid: 19467048
references.citation: Z Naturforsch C J Biosci. 2007 Sep-Oct;62(9-10):668-72
references.pmid: 18069238
references.citation: Biol Pharm Bull. 2004 Oct;27(10):1576-9
references.pmid: 15467198