title: Reverse transcriptase dideoxy sequencing of RNA.
abstract: During the course of studying the structure and function of RNA molecules and the proteins that they may encode, as well as studies of the expression of a given gene, there often arises a need to learn the nucleotide sequence of an RNA molecule. While the sequence of a particular RNA is ordinarily encompassed within a corresponding DNA sequence, RNA sequencing lends the advantage of giving the transcription initiation and termination sequences, and the sites where modifications from the original DNA sequence occur.
authors.affiliation: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Walker
authors.forename: Jon
authors.initials: J
authors.lastname: Lorsch
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D012313:RNA / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D017423:Sequence Analysis, RNA / Q000379:methods*; D014508:Urea / Q000737:chemistry
chemical_list: D012313:RNA; D014508:Urea; D012194:RNA-Directed DNA Polymerase
keywords: Anneal primer; Label primer; Primer extension and chain termination; Reverse transcriptase dideoxy sequencing; UREA-PAGE