title: Pilot study using proteomics to identify predictive biomarkers of necrotizing enterocolitis from buccal swabs in very low birth weight infants.
abstract: BACKGROUND
  Necrotizing enterocolitis (NEC) is a major cause of death and morbidity in very low birth weight infants.
  
  
  OBJECTIVE
  To identify biomarker(s) that would predict NEC using buccal swab samples utilizing a proteomic approach.
  
  
  METHODS
  Cumulative buccal swab samples derived from very low birth weight preterm infants (<32 weeks' gestational age and <1,250 g) at 1, 2 and 3 weeks prior to the development of NEC and matched controls were subjected to two-dimensional difference gel electrophoresis and LC-MS/MS analysis for proteomic protein discovery. After identification of 21 altered proteins, we chose 3 candidate proteins using a broad systems biologic analysis approach that suggested several altered cellular processes that could be associated with NEC.
  
  
  RESULTS
  Preliminary validation studies using Western blots on these samples and 10 additional NEC and 10 matched control buccal samples collected within 2 or 3 weeks before NEC diagnosis analysis showed lower interleukin-1 receptor antagonist.
  
  
  CONCLUSIONS
  Our results suggest that interleukin-1 receptor antagonist is worthy of further studies to determine its utility in helping predict NEC.
authors.affiliation: Division of Neonatology, Department of Pediatrics, University of Florida, Gainesville, Fla., USA.
authors.forename: Roberto
authors.initials: R
authors.lastname: Murgas Torrazza
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Christopher
authors.initials: C
authors.lastname: Young
authors.forename: Firas
authors.initials: F
authors.lastname: Kobeissy
authors.forename: Marjorie
authors.initials: M
authors.lastname: Chow
authors.forename: Sixue
authors.initials: S
authors.lastname: Chen
authors.forename: Volker
authors.initials: V
authors.lastname: Mai
authors.forename: Renu
authors.initials: R
authors.lastname: Sharma
authors.forename: Mark
authors.initials: M
authors.lastname: Hudak
authors.forename: Jonathan
authors.initials: J
authors.lastname: Shuster
authors.forename: Josef
authors.initials: J
authors.lastname: Neu
mesh_terms: D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D002853:Chromatography, Liquid; D020345:Enterocolitis, Necrotizing / Q000175:diagnosis* / Q000378:metabolism; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D019102:Infant, Very Low Birth Weight; D008297:Male; D009061:Mouth Mucosa / Q000737:chemistry*; D010865:Pilot Projects; D011237:Predictive Value of Tests; D040901:Proteomics / Q000379:methods*; D049490:Systems Biology; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers
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