title: Non-lethal heat shock increased Hsp70 and immune protein transcripts but not Vibrio tolerance in the white-leg shrimp.
abstract: Non-lethal heat shock boosts bacterial and viral disease tolerance in shrimp, possibly due to increases in endogenous heat shock protein 70 (Hsp70) and/or immune proteins. To further understand the mechanisms protecting shrimp against infection, Hsp70 and the mRNAs encoding the immune-related proteins prophenoloxidase (proPO), peroxinectin, penaeidin, crustin and hemocyanin were studied in post-larvae of the white-leg shrimp Litopenaeus vannamei, following a non-lethal heat shock. As indicated by RT-qPCR, a 30 min abrupt heat shock increased Hsp70 mRNA in comparison to non-heated animals. Immunoprobing of western blots and quantification by ELISA revealed that Hsp70 production after heat shock was correlated with enhanced Hsp70 mRNA. proPO and hemocyanin mRNA levels were augmented, whereas peroxinectin and crustin mRNA levels were unchanged following non-lethal heat shock. Penaeidin mRNA was decreased by all heat shock treatments. Thirty min abrupt heat shock failed to improve survival of post-larvae in a standardized challenge test with Vibrio harveyi, indicating that under the conditions of this study, L. vannamei tolerance to Vibrio infection was influenced neither by Hsp70 accumulation nor the changes in the immune-related proteins, observations dissimilar to other shrimp species examined.
authors.affiliation: Department of Aquaculture Science, Faculty of Fisheries and Aqua-Industry, Universiti Malaysia Terengganu, Kuala Terengganu, Malaysia.
authors.forename: Nguyen Hong
authors.initials: NH
authors.lastname: Loc
authors.forename: Thomas H
authors.initials: TH
authors.lastname: Macrae
authors.forename: Najiah
authors.initials: N
authors.lastname: Musa
authors.forename: Muhd Danish Daniel
authors.initials: MD
authors.lastname: Bin Abdullah
authors.forename: Mohd Effendy
authors.initials: ME
authors.lastname: Abdul Wahid
authors.forename: Yeong Yik
authors.initials: YY
authors.lastname: Sung
mesh_terms: D000818:Animals; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D018869:Heat-Shock Response / Q000235:genetics* / Q000276:immunology; D033561:Penaeidae / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014733:Vibrio / Q000276:immunology; D014735:Vibrio Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology
chemical_list: D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger
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