title: Biological removal of nitrate and ammonium under aerobic atmosphere by Paracoccus versutus LYM.
abstract: The bacterium isolated from sea sludge Paracoccus versutus LYM was characterized with the ability of aerobic denitrification. Strain LYM performs perfect activity in aerobically converting over 95% NO3(-)-N (approximate 400mg L(-1)) to gaseous products via nitrite with maximum reduction rate 33 mg NO3(-)-N L(-1) h(-1). Besides characteristic of aerobic denitrification, strain LYM was confirmed in terms of the ability to be heterotrophic nitrification and aerobic denitrification (HNAD) with few accumulations of intermediates. After the nitrogen balance and enzyme assays, the putative nitrogen pathway of HNAD could be NH4(+) → NH2OH → NO2(-)→ NO3(-), then NO3(-) was denitrified to gaseous products via nitrite. N2 was sole denitrification product without any detection of N2O by gas chromatography. Strain LYM could also simultaneously remove ammonium and additional nitrate. Meanwhile, the accumulated nitrite had inhibitory effect on ammonium reduction rate.
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Shi
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jiti
authors.initials: J
authors.lastname: Zhou
authors.forename: Mingxiang
authors.initials: M
authors.lastname: Chen
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Wang
mesh_terms: D000332:Aerobiosis / Q000187:drug effects; D064751:Ammonium Compounds / Q000302:isolation & purification*; D001673:Biodegradation, Environmental / Q000187:drug effects; D058440:Denitrification / Q000187:drug effects; D052836:Heterotrophic Processes / Q000187:drug effects; D008969:Molecular Sequence Data; D009566:Nitrates / Q000302:isolation & purification* / Q000494:pharmacology; D058465:Nitrification / Q000187:drug effects; D010230:Paracoccus / Q000187:drug effects / Q000378:metabolism*
chemical_list: D064751:Ammonium Compounds; D009566:Nitrates
keywords: Aerobic denitrification; Heterotrophic nitrification and aerobic denitrification; Nitrogen removal; Paracoccus versutus