title: M-cadherin-mediated intercellular interactions activate satellite cell division.
abstract: Adult muscle stem cells and their committed myogenic precursors, commonly referred to as the satellite cell population, are involved in both muscle growth after birth and regeneration after damage. It has been previously proposed that, under these circumstances, satellite cells first become activated, divide and differentiate, and only later fuse to the existing myofiber through M-cadherin-mediated intercellular interactions. Our data show that satellite cells fuse with the myofiber concomitantly to cell division, and only when the nuclei of the daughter cells are inside the myofiber, do they complete the process of differentiation. Here we demonstrate that M-cadherin plays an important role in cell-to-cell recognition and fusion, and is crucial for cell division activation. Treatment of satellite cells with M-cadherin in vitro stimulates cell division, whereas addition of anti-M-cadherin antibodies reduces the cell division rate. Our results suggest an alternative model for the contribution of satellite cells to muscle development, which might be useful in understanding muscle regeneration, as well as muscle-related dystrophies.
authors.affiliation: Center of Regenerative Medicine in Barcelona, Dr. Aiguader, 88, 08003 Barcelona, Spain.
authors.forename: Merce
authors.initials: M
authors.lastname: Marti
authors.forename: Núria
authors.initials: N
authors.lastname: Montserrat
authors.forename: Cristina
authors.initials: C
authors.lastname: Pardo
authors.forename: Lola
authors.initials: L
authors.lastname: Mulero
authors.forename: Laia
authors.initials: L
authors.lastname: Miquel-Serra
authors.forename: Alexandre Miguel Cavaco
authors.initials: AM
authors.lastname: Rodrigues
authors.forename: José
authors.initials: J
authors.lastname: Andrés Vaquero
authors.forename: Bernd
authors.initials: B
authors.lastname: Kuebler
authors.forename: Cristina
authors.initials: C
authors.lastname: Morera
authors.forename: María José
authors.initials: MJ
authors.lastname: Barrero
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Izpisua Belmonte
mesh_terms: D000818:Animals; D000906:Antibodies; D015820:Cadherins / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002450:Cell Communication / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D016189:Dystrophin / Q000235:genetics; D006801:Humans; D051379:Mice; D024510:Muscle Development / Q000235:genetics*; D018482:Muscle, Skeletal / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D014981:Xenopus
chemical_list: D000906:Antibodies; D015820:Cadherins; D016189:Dystrophin; C070896:M-cadherin
keywords: Cell division; Dystrophin; M-cadherin; Myogenesis; Satellite cells