title: Clusters of cerebellar Purkinje cells control their afferent climbing fiber discharge.
abstract: Climbing fibers, the projections from the inferior olive to the cerebellar cortex, carry sensorimotor error and clock signals that trigger motor learning by controlling cerebellar Purkinje cell synaptic plasticity and discharge. Purkinje cells target the deep cerebellar nuclei, which are the output of the cerebellum and include an inhibitory GABAergic projection to the inferior olive. This pathway identifies a potential closed loop in the olivo-cortico-nuclear network. Therefore, sets of Purkinje cells may phasically control their own climbing fiber afferents. Here, using in vitro and in vivo recordings, we describe a genetically modified mouse model that allows the specific optogenetic control of Purkinje cell discharge. Tetrode recordings in the cerebellar nuclei demonstrate that focal stimulations of Purkinje cells strongly inhibit spatially restricted sets of cerebellar nuclear neurons. Strikingly, such stimulations trigger delayed climbing-fiber input signals in the stimulated Purkinje cells. Therefore, our results demonstrate that Purkinje cells phasically control the discharge of their own olivary afferents and thus might participate in the regulation of cerebellar motor learning.
authors.affiliation: Institut des Neurosciences Cellulaires et Intégratives, Centre National de la Recherche Scientifique, Unité Propre de Recherche 3212, Université de Strasbourg, 67084 Strasbourg, France.
authors.forename: Joseph
authors.initials: J
authors.lastname: Chaumont
authors.forename: Nicolas
authors.initials: N
authors.lastname: Guyon
authors.forename: Antoine M
authors.initials: AM
authors.lastname: Valera
authors.forename: Guillaume P
authors.initials: GP
authors.lastname: Dugué
authors.forename: Daniela
authors.initials: D
authors.lastname: Popa
authors.forename: Paikan
authors.initials: P
authors.lastname: Marcaggi
authors.forename: Vanessa
authors.initials: V
authors.lastname: Gautheron
authors.forename: Sophie
authors.initials: S
authors.lastname: Reibel-Foisset
authors.forename: Stéphane
authors.initials: S
authors.lastname: Dieudonné
authors.forename: Aline
authors.initials: A
authors.lastname: Stephan
authors.forename: Michel
authors.initials: M
authors.lastname: Barrot
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Cassel
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Dupont
authors.forename: Frédéric
authors.initials: F
authors.lastname: Doussau
authors.forename: Bernard
authors.initials: B
authors.lastname: Poulain
authors.forename: Fekrije
authors.initials: F
authors.lastname: Selimi
authors.forename: Clément
authors.initials: C
authors.lastname: Léna
authors.forename: Philippe
authors.initials: P
authors.lastname: Isope
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000166:cytology*; D000075402:Channelrhodopsins; D004525:Efferent Pathways / Q000166:cytology*; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D009847:Olivary Nucleus / Q000166:cytology*; D062308:Optogenetics; D011689:Purkinje Cells / Q000502:physiology*; D045442:Rotarod Performance Test
chemical_list: D000075402:Channelrhodopsins
keywords: complex spikes; motor control; olivo-cerebellar loop
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