title: Allele-specific polymerase chain reaction for detection of a mutation in the relax circular DNA and the covalently closed circular DNA of hepatitis B virus.
abstract: The relax circle DNA (rcDNA) sequence and the covalently closed circle DNA (cccDNA) sequence in hepatitis B virus (HBV) are crucial regions for HBV infections. To analyze mutations in rcDNA and cccDNA, DNA sequencing is often used, although it is time-consuming and expensive. Herein, we report a simple, economic, albeit accurate allele-specific polymerase chain reaction (AS-PCR) to detect mutations in these regions of HBV. This method can be extensively used to screen for mutations at specific positions of HBV genome.
authors.affiliation: Key Laboratory of Molecular Biology on Infection Diseases of the Ministry of Education, Chongqing Medical University, Chongqing 400016, China; Department of Microbiology and Immunology, North Sichuan Medical College, Nanchong 637000, China.
authors.forename: Wan-Long
authors.initials: WL
authors.lastname: Pan
authors.forename: Jie-Li
authors.initials: JL
authors.lastname: Hu
authors.forename: Yan
authors.initials: Y
authors.lastname: Fang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Luo
authors.forename: Ge
authors.initials: G
authors.lastname: Xu
authors.forename: Lei
authors.initials: L
authors.lastname: Xu
authors.forename: Zhou-Hong
authors.initials: ZH
authors.lastname: Jing
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Shan
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Zhu
authors.forename: Ai-Long
authors.initials: AL
authors.lastname: Huang
mesh_terms: D000483:Alleles; D003365:Costs and Cost Analysis; D004270:DNA, Circular / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D008403:Mass Screening / Q000379:methods; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D012680:Sensitivity and Specificity; D014773:Virology / Q000191:economics / Q000379:methods*
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral
keywords: Allele-specific polymerase chain reaction; Point mutation