title: Lack of the host membrane protease FtsH hinders release of the Lactococcus lactis bacteriophage TP712.
abstract: The temperate bacteriophage TP712 was unable to plaque on Lactococcus lactis ΔftsH lacking the membrane protease FtsH and complementation in trans restored the WT phenotype. Absence of ftsH did not hinder phage adsorption, phage DNA delivery or activation of the lytic cycle. Thin sections revealed that TP712 virions appeared to be correctly assembled inside the ΔftsH host, but were not released. These virions were infective, demonstrating that a functional host FtsH is required by TP712 to proceed effectively with lysis of the host.
authors.affiliation: DairySafe Group, Department of Technology and Biotechnology of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Villaviciosa, Asturias, Spain.
authors.forename: Clara
authors.initials: C
authors.lastname: Roces
authors.affiliation: Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom.
authors.forename: Udo
authors.initials: U
authors.lastname: Wegmann
authors.affiliation: DairySafe Group, Department of Technology and Biotechnology of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Villaviciosa, Asturias, Spain.
authors.forename: Ana B
authors.initials: AB
authors.lastname: Campelo
authors.affiliation: DairySafe Group, Department of Technology and Biotechnology of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Villaviciosa, Asturias, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: García
authors.affiliation: DairySafe Group, Department of Technology and Biotechnology of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Villaviciosa, Asturias, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Rodríguez
authors.affiliation: DairySafe Group, Department of Technology and Biotechnology of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Villaviciosa, Asturias, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Martínez
mesh_terms: D049069:ATP-Dependent Proteases / Q000235:genetics* / Q000378:metabolism; D001435:Bacteriophages / Q000378:metabolism / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D013294:Lactococcus lactis / Q000502:physiology* / Q000821:virology; D008242:Lysogeny; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014771:Virion / Q000378:metabolism; D057074:Virus Release
chemical_list: D029968:Escherichia coli Proteins; D049069:ATP-Dependent Proteases; C513383:FtsH protein, E coli