title: Pentamethylquercetin reduces fat deposition via Sirt1-mediated pathways in male obese mice induced by a high fat diet.
abstract: Pentamethylquercetin (PMQ) is a natural quercetin derivative found in a variety of edible herb. Although PMQ has potential as anti-diabetic agent, there have been no reports on its anti-adipogenic effects in the obese animals. This study investigated whether PMQ attenuates high-fat diet (HFD)-induced adipogenesis in the epididymal fat tissues of mice and explored its underlying mechanisms. In comparison with HFD-fed mice, mice fed with PMQ showed significantly lower body weight gain, adipose tissue mass, and plasma levels of triglyceride, total cholesterol, low-density lipoprotein cholesterol, and glucose, but higher plasma levels of high-density lipoprotein cholesterol. PMQ significantly reversed the HFD-induced regulation of Sirt1/mTOR signaling genes (Sirt1, mTOR, 4EBP1, and S6K1), and key adipogenic genes (PPARγ, SREBP1, FAS, ATGL, HSL, and Perilipin) in the epididymal adipose tissues of obese mice. However, nicotinamide appeared to partly inhibit PMQ-mediated anti-adipogenic effects involved in this attenuation. These results suggested that PMQ inhibited visceral adipogenesis by suppressing the Sirt1-mediated mTOR and adipogenesis signaling cascades. It might be a potential candidate for the treatment of obesity.
authors.affiliation: Zhejiang Academy of Medical Sciences, Hangzhou 310013, China. Electronic address: Jellycook2002@163.com.
authors.forename: Hua-Zong
authors.initials: HZ
authors.lastname: Ying
authors.forename: Jia-Na
authors.initials: JN
authors.lastname: Zang
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Deng
authors.forename: Zhi-Yuan
authors.initials: ZY
authors.lastname: Wang
authors.forename: Chen-huan
authors.initials: CH
authors.lastname: Yu
mesh_terms: D050156:Adipogenesis / Q000187:drug effects; D000273:Adipose Tissue / Q000187:drug effects*; D052436:Adipose Tissue, White / Q000187:drug effects; D000818:Animals; D059305:Diet, High-Fat; D005786:Gene Expression Regulation / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009536:Niacinamide / Q000494:pharmacology; D009765:Obesity / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology; D015398:Signal Transduction; D056564:Sirtuin 1 / Q000378:metabolism*; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D014280:Triglycerides / Q000097:blood; D015430:Weight Gain / Q000187:drug effects
chemical_list: C548139:3,3',4',5,7-pentamethylquercetin; D014280:Triglycerides; D009536:Niacinamide; D011794:Quercetin; C546843:mTOR protein, mouse; D058570:TOR Serine-Threonine Kinases; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1
keywords: Mammalian target of rapamycin (mTOR); Obesity; Pentamethylquercetin; Sirtuin1 (Sirt1)