title: Comparison of the effect of intra-tendon applications of recombinant human platelet-derived growth factor-BB, platelet-rich plasma, steroids in a rat achilles tendon collagenase model.
abstract: This study compared the effect of intra-tendon (IT) delivery of recombinant human platelet-derived growth factor-BB (rhPDGF-BB), platelet-rich plasma (PRP) and corticosteroids in a rat tendinopathy model. Seven days after collagenase induction of tendinopathy, a 30-µl IT injection was administered. Treatments included: saline; 3 µg rhPDGF-BB; 10 µg rhPDGF-BB; PRP; and 300 µg triamcinolone acetonide (TCA). Outcomes were assessed 7 and 21 days after treatment. All groups exhibited good to excellent repair. Relative to saline, cell proliferation increased 65% in the 10 µg rhPDGF-BB group and decreased 74% in the TCA group; inflammation decreased 65% in the TCA group. At 7 days, maximum load-to-failure was increased in the 3 µg rhPDGF-BB group relative to saline, PRP, and TCA (p < 0.025). On day 21, maximum load-to-rupture was increased in the 10 µg rhPDGF-BB group relative to saline, PRP, and TCA (p < 0.035) and in the 3 µg rhPDGF-BB group compared to saline and TCA (p < 0.027). Stiffness in the 10 µg rhPDGF-BB group was increased compared to saline, PRP, and TCA (p < 0.038). Histology demonstrated similar repair in all groups. PRP and TCA did not improve mechanical properties compared to saline. Injections of rhPDGF-BB increased maximum load-to-failure (3 and 10 µg) and stiffness (10 µg) relative to controls and commonly used treatments. © 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:145-150, 2014.
authors.affiliation: Research and Development, BioMimetic Therapeutics, LLC, Franklin, Tennessee.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Solchaga
authors.forename: Alison
authors.initials: A
authors.lastname: Bendele
authors.forename: Vivek
authors.initials: V
authors.lastname: Shah
authors.forename: Leo B
authors.initials: LB
authors.lastname: Snel
authors.forename: Hans K
authors.initials: HK
authors.lastname: Kestler
authors.forename: Joshua S
authors.initials: JS
authors.lastname: Dines
authors.forename: Christopher K
authors.initials: CK
authors.lastname: Hee
mesh_terms: D000125:Achilles Tendon / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D000305:Adrenal Cortex Hormones / Q000494:pharmacology*; D000818:Animals; D000077214:Becaplermin; D001696:Biomechanical Phenomena / Q000187:drug effects / Q000502:physiology; D004195:Disease Models, Animal; D006801:Humans; D015552:Injections, Intralesional; D008297:Male; D053657:Platelet-Rich Plasma; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D052256:Tendinopathy / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D016896:Treatment Outcome
chemical_list: D000305:Adrenal Cortex Hormones; D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins; D000077214:Becaplermin
keywords: biomechanics; corticosteroid; platelet-derived growth factor-BB; platelet-rich plasma; tendinopathy