title: Insectivorous bats digest chitin in the stomach using acidic mammalian chitinase.
abstract: The gastrointestinal tract of animals is adapted to their primary source of food to optimize resource use and energy intake. Temperate bat species mainly feed on arthropods. These contain the energy-rich carbohydrate chitin, which is indigestible for the endogenous enzymes of a typical mammalian gastrointestinal tract. However, the gastrointestinal tract of bat species should be adapted to their diet and be able to digest chitin. We hypothesized that (i) European vespertilionid bat species have the digestive enzyme chitinase and that (ii) the chitinolytic activity is located in the intestine, as has been found for North American bat species. The gastrointestinal tracts of seven bat species (Pipistrellus pipistrellus, Plecotus auritus, Myotis bechsteinii, Myotis nattereri, Myotis daubentonii, Myotis myotis, and Nyctalus leisleri) were tested for chitinolytic activity by diffusion assay. Gastrointestinal tracts of P. pipistrellus, P. auritus, M. nattereri, M. myotis, and N. leisleri were examined for acidic mammalian chitinase by western blot analysis. Tissue sections of the gastrointestinal tract of P. pipistrellus were immunohistochemically analyzed to locate the acidic mammalian chitinase. Chitinolytic activity was detected in the stomachs of all bat species. Western blot analysis confirmed the acidic mammalian chitinase in stomach samples. Immunohistochemistry of the P. pipistrellus gastrointestinal tract indicated that acidic mammalian chitinase is located in the stomach chief cells at the base of the gastric glands. In conclusion, European vespertilionid bat species have acidic mammalian chitinase that is produced in the gastric glands of the stomach. Therefore, the gastrointestinal tracts of insectivorous bat species evolved an enzymatic adaptation to their diet.
authors.affiliation: Mammalian Ecology Group, Department of Animal Ecology and Systematics, Justus-Liebig-University of Giessen, Giessen, Germany ; Department of General Zoology and Developmental Biology, Justus-Liebig-University of Giessen, Giessen, Germany.
authors.forename: Sara
authors.initials: S
authors.lastname: Strobel
authors.forename: Anna
authors.initials: A
authors.lastname: Roswag
authors.forename: Nina I
authors.initials: NI
authors.lastname: Becker
authors.forename: Tina E
authors.initials: TE
authors.lastname: Trenczek
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Encarnação
mesh_terms: D000143:Acids / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002685:Chiroptera / Q000502:physiology*; D002686:Chitin / Q000378:metabolism*; D002688:Chitinases / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D006868:Hydrolysis; D007313:Insecta
chemical_list: D000143:Acids; D002686:Chitin; D002688:Chitinases
references.citation: J Histochem Cytochem. 2002 Aug;50(8):1081-9
references.pmid: 12133911
references.citation: J Cell Biol. 1963 Mar;16:541-77
references.pmid: 13957001
references.citation: Eur J Morphol. 1998 Aug;36 Suppl:19-26
references.pmid: 9825887
references.citation: Nature. 1970 Aug 15;227(5259):680-5
references.pmid: 5432063
references.citation: World J Gastroenterol. 2009 Nov 14;15(42):5249-59
references.pmid: 19908331
references.citation: Nature. 1961 Oct 14;192:135-6
references.pmid: 14451758
references.citation: J Histochem Cytochem. 2005 Oct;53(10):1283-92
references.pmid: 15923370
references.citation: Biochim Biophys Acta. 1957 Dec;26(3):517-21
references.pmid: 13499409
references.citation: Am J Clin Nutr. 1992 Jan;55(1 Suppl):299S-308S
references.pmid: 1728844
references.citation: J Microbiol Methods. 2004 Feb;56(2):213-9
references.pmid: 14744450
references.citation: Int Immunol. 2005 Nov;17(11):1505-12
references.pmid: 16214810
references.citation: J Anat. 1985 Oct;142:141-50
references.pmid: 17103582
references.citation: Genetics. 2007 Oct;177(2):959-70
references.pmid: 17720922
references.citation: J Natl Med Assoc. 1924 Jan;16(1):5-8
references.pmid: 20892069
references.citation: Mol Cell Biochem. 1984 Sep;63(2):165-89
references.pmid: 6387440
references.citation: J Biol Chem. 2001 Mar 2;276(9):6770-8
references.pmid: 11085997
references.citation: J Biosci. 2010 Mar;35(1):127-60
references.pmid: 20413917
references.citation: Comp Biochem Physiol A Mol Integr Physiol. 2012 Aug;162(4):386-90
references.pmid: 22561664
references.citation: Folia Microbiol (Praha). 2001;46(1):76-8
references.pmid: 11501484
references.citation: Trends Pharmacol Sci. 2004 Oct;25(10):509-11
references.pmid: 15380933
references.citation: Mol Biotechnol. 2002 Sep;22(1):19-23
references.pmid: 12353911
references.citation: Gene. 2010 Mar 1;452(2):63-71
references.pmid: 20036323
references.citation: Curr Opin Biotechnol. 1998 Jun;9(3):270-7
references.pmid: 9650272
references.citation: Arch Oral Biol. 2003 Oct;48(10):701-7
references.pmid: 12971947