title: Porous hydrogels from shark skin collagen crosslinked under dense carbon dioxide atmosphere.
abstract: The possibility to fabricate marine collagen porous structures crosslinked with genipin under high pressure carbon dioxide is investigated. Collagen from shark skin is used to prepare pre-scaffolds by freeze-drying. The poor stability of the structures and low mechanical properties require crosslinking of the structures. Under dense CO2 atmosphere, crosslinking of collagen pre-scaffolds is allowed for 16 h. Additionally, the hydrogels are foamed and the scaffolds obtained present a highly porous structure. In vitro cell culture tests performed with a chondrocyte-like cell line show good cell adherence and proliferation, which is a strong indication of the potential of these scaffolds to be used in tissue cartilage tissue engineering.
authors.affiliation: 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineAvePark, 4806-909, Taipas, Guimarães, Portugal; ICVS/3B's - PT Government Associate LaboratoryBraga/Guimarães, Portugal.
authors.forename: Susana
authors.initials: S
authors.lastname: Fernandes-Silva
authors.forename: Joana
authors.initials: J
authors.lastname: Moreira-Silva
authors.forename: Tiago H
authors.initials: TH
authors.lastname: Silva
authors.forename: Ricardo I
authors.initials: RI
authors.lastname: Perez-Martin
authors.forename: Carmen G
authors.initials: CG
authors.lastname: Sotelo
authors.forename: João F
authors.initials: JF
authors.lastname: Mano
authors.forename: Ana Rita C
authors.initials: AR
authors.lastname: Duarte
authors.forename: Rui L
authors.initials: RL
authors.lastname: Reis
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects; D003094:Collagen / Q000737:chemistry* / Q000302:isolation & purification; D003432:Cross-Linking Reagents / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005612:Freeze Drying; D020100:Hydrogels / Q000737:chemistry* / Q000494:pharmacology; D039823:Iridoids / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D016062:Porosity; D012754:Sharks; D012867:Skin / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D020100:Hydrogels; D039823:Iridoids; D002245:Carbon Dioxide; D003094:Collagen; C007834:genipin
keywords: collagen; hydrogels; marine biotechnology; supercritical fluids; tissue engineering