title: The deSUMOylase SENP7 promotes chromatin relaxation for homologous recombination DNA repair.
abstract: SUMO conjugation is known to occur in response to double-stranded DNA breaks in mammalian cells, but whether SUMO deconjugation has a role remains unclear. Here, we show that the SUMO/Sentrin/Smt3-specific peptidase, SENP7, interacts with the chromatin repressive KRAB-associated protein 1 (KAP1) through heterochromatin protein 1 alpha (HP1α). SENP7 promotes the removal of SUMO2/3 from KAP1 and regulates the interaction of the chromatin remodeler CHD3 with chromatin. Consequently, in the presence of CHD3, SENP7 is required for chromatin relaxation in response to DNA damage, for homologous recombination repair and for cellular resistance to DNA-damaging agents. Thus, deSUMOylation by SENP7 is required to promote a permissive chromatin environment for DNA repair.
authors.affiliation: School of Cancer Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT.
authors.forename: Alexander J
authors.initials: AJ
authors.lastname: Garvin
authors.forename: Ruth M
authors.initials: RM
authors.lastname: Densham
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Blair-Reid
authors.forename: Kenny M
authors.initials: KM
authors.lastname: Pratt
authors.forename: Helen R
authors.initials: HR
authors.lastname: Stone
authors.forename: Daniel
authors.initials: D
authors.lastname: Weekes
authors.forename: Kirsty J
authors.initials: KJ
authors.lastname: Lawrence
authors.forename: Joanna R
authors.initials: JR
authors.lastname: Morris
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D002843:Chromatin / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone; D053903:DNA Breaks, Double-Stranded; D004249:DNA Damage; D004265:DNA Helicases / Q000378:metabolism; D010450:Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D056245:Mi-2 Nucleosome Remodeling and Deacetylase Complex / Q000378:metabolism; D008969:Molecular Sequence Data; D011485:Protein Binding; D059767:Recombinational DNA Repair; D025842:SUMO-1 Protein / Q000378:metabolism
chemical_list: C000715594:CBX5 protein, human; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D025842:SUMO-1 Protein; D000090266:Chromobox Protein Homolog 5; D010450:Endopeptidases; C533959:SENP7 protein, human; D056245:Mi-2 Nucleosome Remodeling and Deacetylase Complex; D004265:DNA Helicases; C115322:CHD3 protein, human
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