title: Indium chloride-induced micronuclei via reactive oxygen species in Chinese hamster lung fibroblast V79 cells.
abstract: We study the cytotoxicity of indium chloride (InCl₃) in Chinese hamster lung fibroblasts, the V79 cells, using MTT assay. The results showed that InCl₃ did not induce significant cytotoxicity at various concentrations tested. In addition, the frequency of micronuclei (MN) was assayed to evaluate the genotoxic effects of InCl₃ in V79 cells. InCl₃ at concentrations ranged 0.1-1 μM significantly increased MN frequency in a concentration-dependent manner. Both catalase and superoxide dismutase at concentrations of 75 and 150 μg/mL significantly inhibited InCl₃-induced MN. Similarly, Germanium oxide (GeO₂) and dimercaprol expressed antigenotoxic effects. From these findings, it is concluded that InCl₃ is a potent genotoxic chemical, which may be mediated partly by inducing oxidative stress. The significance of this study shows that the workers in the semiconductor factories should be cautious in exposing to the hazardous genotoxic InCl₃.
authors.affiliation: Department of Pharmacology, School of Medicine, Chung Shan Medical University and Chung Shan Medical University Hospital, Taichung, Taiwan, Republic of China; Institute of Medicine, College of Medicine, Chung Shan Medical University, Taichung, Taiwan, Republic of China.
authors.forename: Ruey-Hseng
authors.initials: RH
authors.lastname: Lin
authors.forename: Ming-Ling
authors.initials: ML
authors.lastname: Yang
authors.forename: Yi-Ching
authors.initials: YC
authors.lastname: Li
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Chang
authors.forename: Yu-Hsiang
authors.initials: YH
authors.lastname: Kuan
mesh_terms: D000818:Animals; D002374:Catalase / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D004112:Dimercaprol / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D005857:Germanium / Q000494:pharmacology; D007204:Indium / Q000633:toxicity*; D008168:Lung / Q000166:cytology*; D015162:Micronucleus Tests; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000494:pharmacology
chemical_list: D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D005857:Germanium; D007204:Indium; D004112:Dimercaprol; C020758:indium trichloride; C040516:germanium oxide; D002374:Catalase; D013482:Superoxide Dismutase
keywords: InCl3; catalase; micronuclei; superoxide dismutase