title: Variable selection methods for developing a biomarker panel for prediction of dengue hemorrhagic fever.
abstract: BACKGROUND
  The choice of selection methods to identify important variables for binary classification modeling is critical to produce stable models that are interpretable, that generate accurate predictions and have minimum bias. This work is motivated by data on clinical and laboratory features of severe dengue infections (dengue hemorrhagic fever, DHF) obtained from 51 individuals enrolled in a prospective observational study of acute human dengue infections.
  
  
  RESULTS
  We carry out a comprehensive performance comparison using several classification models for DHF over the dengue data set. We compared variable selection results by Multivariate Adaptive Regression Splines, Learning Ensemble, Random Forest, Bayesian Moving Averaging, Stochastic Search Variable Selection, and Generalized Regularized Logistics Regression. Model averaging methods (bagging, boosting and ensemble learners) have higher accuracy, but the generalized regularized regression model has the highest predictive power because the linearity assumptions of candidate predictors are strongly satisfied via deviance chi-square testing procedures. Bootstrapping applications for evaluating predictive regression coefficients in regularized regression model are performed.
  
  
  CONCLUSIONS
  Feature reduction methods introduce inherent biases and therefore are data-type dependent. We propose that these limitations can be overcome using an exhaustive approach for searching feature space. Using this approach, our results suggest that IL-10, platelet and lymphocyte counts are the major features for predicting dengue DHF on the basis of blood chemistries and cytokine measurements.
authors.affiliation: Departments of Preventive Medicine and Community Health, University of Texas Medical Branch (UTMB), Galveston, TX, USA. hyju@utmb.edu.
authors.forename: Hyunsu
authors.initials: H
authors.lastname: Ju
authors.forename: Allan R
authors.initials: AR
authors.lastname: Brasier
mesh_terms: D015415:Biomarkers / Q000032:analysis; D001792:Blood Platelets / Q000276:immunology / Q000473:pathology*; D057225:Data Mining / Q000706:statistics & numerical data*; D006801:Humans; D016753:Interleukin-10 / Q000097:blood*; D016013:Likelihood Functions; D016015:Logistic Models; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000276:immunology / Q000473:pathology*; D010976:Platelet Count; D011379:Prognosis; D011446:Prospective Studies; D019595:Severe Dengue / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology
chemical_list: D015415:Biomarkers; C508609:IL10 protein, human; D016753:Interleukin-10
references.citation: Genet Epidemiol. 2009 Jan;33(1):27-37
references.pmid: 18618760
references.citation: Stat Methods Med Res. 1995 Sep;4(3):197-217
references.pmid: 8548103
references.citation: Stat Methods Med Res. 1995 Sep;4(3):187-96
references.pmid: 8548102
references.citation: Clin Transl Sci. 2012 Feb;5(1):8-20
references.pmid: 22376251
references.citation: Am J Trop Med Hyg. 2012 Feb;86(2):341-8
references.pmid: 22302872
references.citation: Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5116-21
references.pmid: 11309499