title: Distribution of bone-marrow-derived endothelial and immune cells in a murine colitis-associated colorectal cancer model.
abstract: Inflammatory bowel disease (IBD) can lead to an increased risk of developing colorectal cancer (CRC). The aim of this study was to establish a model for combined bone marrow transplantation (BMT) and colitis-associated colorectal cancer (CAC) and to define the contribution of BM-derived cells during the inflammation associated with carcinogenesis. We established a model for BMT using green fluorescent protein (GFP) transgenic mice, followed by AOM/DSS-induced CAC, and performed confocal microscopy analysis on in vivo living tissue and frozen tumor sections. Our imaging analyses showed that GFP-positive cells extensively infiltrated the tumor stroma and that some WGA and GFP or CD31 and GFP double-positive cells were observed in the lining of tumor vessels. Flow cytometry analysis of the tumor-infiltrating cells showed that the GFP-positive CD11c+ DCs cells were one-third of the GFP+/CD11C- cells, and that half of these DCs (0.96% vs 1.02%) were GFP-positive BM-derived cells. The majority of CD4(+) T cells were GFP-negative (12.02% vs 1.9%), and we discovered a novel CD4(+) CD11c(+) DC subset (0.34% vs 1.64%). In conclusion, we defined the distribution of BM-derived endothelial cells, CD11c(+) DCs and CD4(+) T cells in tumors. This model might be useful for elucidating the diverse BM-derived cell types and functions during the progression of colitis-associated colorectal cancer.
authors.affiliation: Department of Gastroenterology, Zhongshan Hospital affiliated to Xiamen University, Xiamen, Fujian Province, China.
authors.forename: Chuan-Xing
authors.initials: CX
authors.lastname: Xiao
authors.forename: Huan-Huan
authors.initials: HH
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Shi
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Liu
authors.forename: Jian-Lin
authors.initials: JL
authors.lastname: Ren
authors.forename: Bayasi
authors.initials: B
authors.lastname: Guleng
mesh_terms: D000818:Animals; D001397:Azoxymethane; D001854:Bone Marrow Cells / Q000378:metabolism*; D016026:Bone Marrow Transplantation / Q000379:methods; D039521:CD11c Antigen / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D002460:Cell Line; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000139:chemically induced / Q000378:metabolism*; D003713:Dendritic Cells / Q000378:metabolism*; D016264:Dextran Sulfate; D042783:Endothelial Cells / Q000378:metabolism*; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: D039521:CD11c Antigen; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D049452:Green Fluorescent Proteins; D016264:Dextran Sulfate; D001397:Azoxymethane
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