title: [Detection of human parvovirus B19, human bocavirus and human parvovirus 4 infections in blood samples among 95 patients with liver disease in Nanjing by nested PCR].
abstract: OBJECTIVE
  To analyze the infection of human parvovirus B19, human bocavirus (HBoV) and human parvovirus 4 (PARV4) in blood samples among patients with liver disease in Nanjing by molecular detection.
  
  
  METHODS
  Nested PCR assays were designed and validated to detect B19, HBoV and PARV4, respectively. The assays were used to screen three parvoviruses in blood samples from 95 patients with different liver disease in Nanjing. The parvovirus infection was analyzed statistically.
  
  
  RESULTS
  The detection limits were 10 copies of genomic DNA equivalents per reaction for each assays and the good specificity were observed. The frequency of B19 and HBoV were 2/95 (2.1%) and 9/95 (9.5%) in blood samples respectively. No PARV4 was detected. HBoV was detected in 3/5 patients with drug-induced hepatitis.
  
  
  CONCLUSIONS
  Both B19 and HBoV infection were detected in blood from patients with liver disease.
authors.affiliation: Institute of Medical Virology, Wenzhou Medical College, Wenzhou 325035, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Tong
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Zhou
authors.forename: Xi-Jun
authors.initials: XJ
authors.lastname: Liu
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Yong-Liang
authors.initials: YL
authors.lastname: Lou
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Tan
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D060085:Coinfection / Q000821:virology*; D005260:Female; D056661:Human bocavirus / Q000302:isolation & purification*; D006801:Humans; D008107:Liver Diseases / Q000821:virology*; D008297:Male; D008875:Middle Aged; D017992:Parvovirus / Q000302:isolation & purification*; D016732:Parvovirus B19, Human / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D014766:Viremia / Q000821:virology*