title: Proteome analysis of functionally differentiated bovine (Bos indicus) mammary epithelial cells isolated from milk.
abstract: Mammary gland is made up of a branching network of ducts that end in alveoli. Terminally differentiated mammary epithelial cells (MECs) constitute the innermost layer of aveoli. They are milk-secreting cuboidal cells that secrete milk proteins during lactation. Little is known about the expression profile of proteins in the metabolically active MECs during lactation or their functional role in the lactation process. In the present investigation, we have reported the proteome map of MECs in lactating cows using 2DE MALDI-TOF/TOF MS and 1D-Gel-LC-MS/MS. MECs were isolated from milk using immunomagnetic beads and confirmed by RT-PCR and Western blotting. The 1D-Gel-LC-MS/MS and 2DE-MS/MS based approaches led to identification of 431 and 134 proteins, respectively, with a total of 497 unique proteins. Proteins identified in this study were clustered into functional groups using bioinformatics tools. Pathway analysis of the identified proteins revealed 28 pathways (p < 0.05) providing evidence for involvement of various proteins in lactation function. This study further provides experimental evidence for the presence of many proteins that have been predicted in annotated bovine genome. The data generated further provide a set of bovine MEC-specific proteins that will help the researchers to understand the molecular events taking place during lactation.
authors.affiliation: Animal Biotechnology Center, National Dairy Research Institute (NDRI), Karnal, India.
authors.forename: Jagadeesh
authors.initials: J
authors.lastname: Janjanam
authors.forename: Manu
authors.initials: M
authors.lastname: Jamwal
authors.forename: Surender
authors.initials: S
authors.lastname: Singh
authors.forename: Saravanan
authors.initials: S
authors.lastname: Kumar
authors.forename: Aswini K
authors.initials: AK
authors.lastname: Panigrahi
authors.forename: Gururao
authors.initials: G
authors.lastname: Hariprasad
authors.forename: Manoj K
authors.initials: MK
authors.lastname: Jena
authors.forename: Vijay
authors.initials: V
authors.lastname: Anand
authors.forename: Sudarshan
authors.initials: S
authors.lastname: Kumar
authors.forename: Jai K
authors.initials: JK
authors.lastname: Kaushik
authors.forename: Ajay K
authors.initials: AK
authors.lastname: Dang
authors.forename: Manishi
authors.initials: M
authors.lastname: Mukesh
authors.forename: Bishnu P
authors.initials: BP
authors.lastname: Mishra
authors.forename: Alagiri
authors.initials: A
authors.lastname: Srinivasan
authors.forename: Vanga S
authors.initials: VS
authors.lastname: Reddy
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Mohanty
mesh_terms: D000818:Animals; D002417:Cattle; D004847:Epithelial Cells / Q000737:chemistry*; D005260:Female; D007774:Lactation / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D008892:Milk / Q000166:cytology*; D060066:Protein Interaction Maps; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000737:chemistry
chemical_list: D011506:Proteins; D020543:Proteome
keywords: Animal proteomics; Bovine; Lactation; MEC; Milk