title: Analysis of isotype-specific antibody responses to bovine herpesviruses 1.1 and 1.2a allows to estimate the stage of infection.
abstract: Specific IgM, IgA, IgG1, IgG2, as well as neutralizing antibody responses were evaluated in sera of calves experimentally infected with two isolates of bovine herpesvirus type 1 (BoHV1) of distinct subtypes (subtype 1, BoHV1.1; subtype 2a, BoHV-1.2a). No significant differences were observed in the antibody responses induced by each BoHV-1 subtype. The antibody responses following primary acute infection were characterized by an increase in specific IgM and IgA levels between days 2 and 14 post inoculation (pi). IgG1 was detected from days 11 to 30 pi. IgG2 was detected on the sample taken on day 30 pi. Reactivation of infection following dexamethasone administration induced a significant rise in IgA levels, whereas IgG1 and IgG2 levels, which were at high levels from the beginning of the reactivation process, showed a slight alteration after corticosteroid treatment. These results suggest that it is possible to estimate the dynamics of BoHV-1 infections with basis on the analysis of class- and subclass-specific antibody responses. Such information may be particularly useful for the study of the kinetics of the infection in a herd and to aid in the adoption of appropriate control measures..
authors.affiliation: Laboratório de Microbiologia Molecular, Instituto de Ciências da Saúde, Universidade Feevale , Novo Hamburgo, RS , Brasil.
authors.forename: Fernando Rosado
authors.initials: FR
authors.lastname: Spilki
authors.forename: Ana Cláudia
authors.initials: AC
authors.lastname: Franco
authors.forename: Paulo Michel
authors.initials: PM
authors.lastname: Roehe
keywords: ELISA; bovine herpesvirus type 1; immunoglobulin subclasses; infectious bovine rhinotracheitis
references.citation: Immunology. 2005 Jan;114(1):101-11
references.pmid: 15606800
references.citation: Arch Virol. 2009;154(1):171-7
references.pmid: 19066710
references.citation: Am J Vet Res. 1985 Apr;46(4):893-8
references.pmid: 2990266
references.citation: Am J Vet Res. 1984 Apr;45(4):790-4
references.pmid: 6329043
references.citation: Vet Microbiol. 1996 Nov;53(1-2):143-51
references.pmid: 9011006
references.citation: Vet Immunol Immunopathol. 1995 Jun;46(3-4):267-83
references.pmid: 7502487
references.citation: Am J Vet Res. 1988 Oct;49(10):1653-6
references.pmid: 2847601
references.citation: J Virol Methods. 2005 Nov;129(2):191-3
references.pmid: 16046004
references.citation: Virus Res. 2008 Jan;131(1):16-22
references.pmid: 17889957
references.citation: Am J Vet Res. 1986 Feb;47(2):223-8
references.pmid: 2420240
references.citation: Vet Microbiol. 2002 Sep 24;88(4):315-24
references.pmid: 12220807
references.citation: Vet Immunol Immunopathol. 1995 Jul;47(1-2):81-92
references.pmid: 8533302