title: Heat-induced antigen retrieval: an effective method to detect and identify progenitor cell types during adult hippocampal neurogenesis.
abstract: Traditional methods of immunohistochemistry (IHC) following tissue fixation allow visualization of various cell types. These typically proceed with the application of antibodies to bind antigens and identify cells with characteristics that are a function of the inherent biology and development. Adult hippocampal neurogenesis is a sequential process wherein a quiescent neural stem cell can become activated and proceed through stages of proliferation, differentiation, maturation and functional integration. Each phase is distinct with a characteristic morphology and upregulation of genes. Identification of these phases is important to understand the regulatory mechanisms at play and any alterations in this process that underlie the pathophysiology of debilitating disorders. Our heat-induced antigen retrieval approach improves the intensity of the signal that is detected and allows correct identification of the progenitor cell type. As discussed in this paper, it especially allows us to circumvent current problems in detection of certain progenitor cell types.
authors.affiliation: Department of Neurologic Surgery, Mayo Clinic College of Medicine.
authors.forename: Syed M Q
authors.initials: SM
authors.lastname: Hussaini
authors.forename: Heechul
authors.initials: H
authors.lastname: Jun
authors.forename: Chang Hoon
authors.initials: CH
authors.lastname: Cho
authors.forename: Hyo Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Woon Ryoung
authors.initials: WR
authors.lastname: Kim
authors.forename: Mi-Hyeon
authors.initials: MH
authors.lastname: Jang
mesh_terms: D000818:Animals; D000941:Antigens / Q000032:analysis*; D006624:Hippocampus / Q000737:chemistry / Q000166:cytology*; D007150:Immunohistochemistry / Q000379:methods*; D051379:Mice; D058953:Neural Stem Cells / Q000737:chemistry / Q000166:cytology*; D055495:Neurogenesis
chemical_list: D000941:Antigens
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