title: Comparison of DNA extraction methods for sweet corn and processed sweet corns.
abstract: DNA was extracted from sweet corn and its processed products using four DNA extraction methods: the CTAB method, the DNeasy Plant Maxi kit, GM Quicker 3, and Genomic-tip 20/G. DNA was successfully extracted from raw sweet corn and baby corn samples using all four methods. Meanwhile, from frozen, canned, and dry pack products, DNA was well extracted using the DNeasy Plant Maxi kit, GM Quicker 3, and Genomic-tip 20/G, but not enough with the CTAB method. The highest yield of DNA was obtained with Genomic-tip 20/G. The degree of degradation of extracted DNA was observed to increase in the order of raw, frozen, canned, dry pack, and baby corn samples. To evaluate the quality of extracted DNA, real-time PCR analyses were conducted using three maize endogenous genes. The DNAs extracted using GM Quicker 3 had high purity, suggesting that GM Quicker 3 would be the most suitable method for DNA extraction from processed sweet corn products.
authors.affiliation: Analytical Science Division, National Food Research Institute, National Agriculture and Food Research Organization.
authors.forename: Reona
authors.initials: R
authors.lastname: Takabatake
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Noritake
authors.forename: Akio
authors.initials: A
authors.lastname: Noguchi
authors.forename: Kosuke
authors.initials: K
authors.lastname: Nakamura
authors.forename: Kazunari
authors.initials: K
authors.lastname: Kondo
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Akiyama
authors.forename: Reiko
authors.initials: R
authors.lastname: Teshima
authors.forename: Junichi
authors.initials: J
authors.lastname: Mano
authors.forename: Kazumi
authors.initials: K
authors.lastname: Kitta
mesh_terms: D018744:DNA, Plant / Q000302:isolation & purification*; D005504:Food Analysis / Q000379:methods*; D005511:Food Handling; D030841:Food, Genetically Modified; D030821:Plants, Genetically Modified; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D003313:Zea mays / Q000235:genetics*
chemical_list: D018744:DNA, Plant