title: Nitrogen starvation affects bacterial adhesion to soil.
abstract: One of the main factors limiting the bioremediation of subsoil environments based on bioaugmentation is the transport of selected microorganisms to the contaminated zones. The characterization of the physiological responses of the inoculated microorganisms to starvation, especially the evaluation of characteristics that affect the adhesion of the cells to soil particles, is fundamental to anticipate the success or failure of bioaugmentation. The objective of this study was to investigate the effect of nitrogen starvation on cell surface hydrophobicity and cell adhesion to soil particles by bacterial strains previously characterized as able to use benzene, toluene or xilenes as carbon and energy sources. The strains LBBMA 18-T (non-identified), Arthrobacter aurescens LBBMA 98, Arthrobacter oxydans LBBMA 201, and Klebsiella sp. LBBMA 204-1 were used in the experiments. Cultivation of the cells in nitrogen-deficient medium caused a significant reduction of the adhesion to soil particles by all the four strains. Nitrogen starvation also reduced significantly the strength of cell adhesion to the soil particles, except for Klebsiella sp. LBBMA 204-1. Two of the four strains showed significant reduction in cell surface hydrophobicity. It is inferred that the efficiency of bacterial transport through soils might be potentially increased by nitrogen starvation.
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Viçosa , Viçosa, MG , Brasil.
authors.forename: Maria Tereza
authors.initials: MT
authors.lastname: Borges
authors.forename: Antônio Galvão
authors.initials: AG
authors.lastname: Nascimento
authors.forename: Ulisses Nunes
authors.initials: UN
authors.lastname: Rocha
authors.forename: Marcos Rogério
authors.initials: MR
authors.lastname: Tótola
keywords: bacterial adhesion; hydrophobicity; nitrogen starvation.
references.citation: Appl Environ Microbiol. 1983 Mar;45(3):1109-15
references.pmid: 16346231
references.citation: Appl Environ Microbiol. 1998 May 1;64(5):1708-14
references.pmid: 9572940
references.citation: Microbiology (Reading). 1997 Jul;143(7):2305-2312
references.pmid: 33657723
references.citation: Appl Environ Microbiol. 1989 Apr;55(4):832-6
references.pmid: 2499255
references.citation: Appl Environ Microbiol. 1991 Sep;57(9):2497-501
references.pmid: 1662934
references.citation: FEMS Microbiol Ecol. 2005 Jan 1;51(2):237-45
references.pmid: 16329872
references.citation: Appl Environ Microbiol. 1993 Oct;59(10):3455-62
references.pmid: 16349075
references.citation: Int J Food Microbiol. 2000 Apr 10;55(1-3):19-25
references.pmid: 10791712
references.citation: Colloids Surf B Biointerfaces. 2005 Mar 10;41(1):33-41
references.pmid: 15698754
references.citation: J Contam Hydrol. 2002 Jun;56(3-4):227-46
references.pmid: 12102320
references.citation: Appl Environ Microbiol. 1991 Nov;57(11):3193-9
references.pmid: 16348583
references.citation: J Contam Hydrol. 2004 May;70(1-2):37-62
references.pmid: 15068868
references.citation: Appl Environ Microbiol. 2000 Sep;66(9):3905-10
references.pmid: 10966407
references.citation: Appl Environ Microbiol. 1999 Nov;65(11):4995-5002
references.pmid: 10543814
references.citation: Appl Environ Microbiol. 1994 Jul;60(7):2637-42
references.pmid: 16349340
references.citation: J Bacteriol. 1994 Jan;176(1):7-14
references.pmid: 8282712
references.citation: J Microbiol Methods. 2001 Jun;45(2):95-101
references.pmid: 11311394
references.citation: Appl Environ Microbiol. 1999 Nov;65(11):5082-8
references.pmid: 10543826
references.citation: Can J Microbiol. 2000 Jan;46(1):28-37
references.pmid: 10696469
references.citation: Curr Biol. 2000 Nov 2;10(21):R777-80
references.pmid: 11084348
references.citation: Ann N Y Acad Sci. 1994 Nov 30;745:61-76
references.pmid: 7832533
references.citation: J Bacteriol. 1996 Feb;178(3):817-22
references.pmid: 8550518
references.citation: FEMS Microbiol Ecol. 2000 Jul 1;33(1):1-9
references.pmid: 10922497
references.citation: Appl Microbiol Biotechnol. 1999 Nov;52(6):839-44
references.pmid: 10616718
references.citation: FEMS Microbiol Rev. 1990 Mar;6(1):19-43
references.pmid: 2186759
references.citation: Appl Environ Microbiol. 1985 Jan;49(1):1-7
references.pmid: 3883894
references.citation: Int J Food Microbiol. 2001 Apr 11;65(1-2):105-11
references.pmid: 11322692