title: Tannase Production by Penicillium Atramentosum KM under SSF and its Applications in Wine Clarification and Tea Cream Solubilization.
abstract: Tannin acyl hydrolase commonly known as tannase is an industrially important enzyme having a wide range of applications, so there is always a scope for novel tannase with better characteristics. A newly isolated tannase-yielding fungal strain identified as Penicillium atramentosum KM was used for tannase production under solid-state fermentation (SSF) using different agro residues like amla (Phyllanthus emblica), ber (Zyzyphus mauritiana), jamun (Syzygium cumini), Jamoa (Eugenia cuspidate) and keekar (Acacia nilotica) leaves. Among these substrates, maximal extracellular tannase production i.e. 170.75 U/gds and 165.56 U/gds was obtained with jamun and keekar leaves respectively at 28ºC after 96 h. A substrate to distilled water ratio of 1:2 (w/v) was found to be the best for tannase production. Supplementation of sodium nitrate (NaNO3) as nitrogen source had enhanced tannase production both in jamun and keekar leaves. Applications of the enzyme were studied in wine clarification and tea cream solubilization. It resulted in 38.05% reduction of tannic acid content in case of jamun wine, 43.59% reduction in case of grape wine and 74% reduction in the tea extract after 3 h at 35°C.
authors.affiliation: Department of Biotechnology, Kurukshetra University , Kurukshetra-136119, Haryana , India.
authors.forename: Manjit K
authors.initials: MK
authors.lastname: Selwal
authors.forename: Anita
authors.initials: A
authors.lastname: Yadav
authors.forename: Krishan K
authors.initials: KK
authors.lastname: Selwal
authors.forename: N K
authors.initials: NK
authors.lastname: Aggarwal
authors.forename: Ranjan
authors.initials: R
authors.lastname: Gupta
authors.forename: S K
authors.initials: SK
authors.lastname: Gautam
keywords: Agro residues; Jamun leaves; Penicillium atramentosum KM; SSF; Tannin acyl hydrolase
references.citation: Acta Microbiol Immunol Hung. 2007 Jun;54(2):159-66
references.pmid: 17899795
references.citation: Anal Biochem. 2001 Aug 15;295(2):168-71
references.pmid: 11488618
references.citation: Appl Environ Microbiol. 1983 Aug;46(2):525-7
references.pmid: 16346377
references.citation: Appl Microbiol Biotechnol. 2007 Nov;77(1):77-89
references.pmid: 17786433
references.citation: J Basic Microbiol. 1999;39(3):161-8
references.pmid: 10427737
references.citation: Appl Biochem Biotechnol. 2007 Apr;137-140(1-12):675-88
references.pmid: 18478425
references.citation: Pol J Microbiol. 2006;55(4):297-301
references.pmid: 17416066
references.citation: Appl Microbiol Biotechnol. 2007 Aug;76(1):47-59
references.pmid: 17530245
references.citation: Bioresour Technol. 2007 Feb;98(3):721-4
references.pmid: 16574410
references.citation: J Microbiol Biotechnol. 2007 Jun;17(6):1049-53
references.pmid: 18050927
references.citation: J Basic Microbiol. 2000;40(4):223-32
references.pmid: 10986668
references.citation: Bioresour Technol. 2005 Jul;96(11):1223-8
references.pmid: 15734308
references.citation: Adv Appl Microbiol. 1997;44:215-60
references.pmid: 9311108