title: Age and heat exposure-dependent changes in antioxidant enzymes activities in rat's liver and brain mitochondria: role of alpha-tocopherol.
abstract: To investigate the role of mitochondrial antioxidant capacity during increased susceptibility to heat accompanied by the aging, young and aged Wistar rats were exposed on heat for 60 min. After heat exposure, hepatic and brain mitochondria were isolated. Our results revealed changes in antioxidant enzyme activities in liver and brain mitochondria from young and to a greater extent in aged rats. Our measurements of MnSOD, GPx and GR activity indicate greater reactive oxygen species production from the mitochondria of aged heat exposed in comparison to young heat exposed rats. Also in the aged rats, the effect of alpha-tocopherol treatment in the prevention of oxidative stress occurred as a result of heat exposure, is less pronounced. Taken together, our data suggest that mitochondria in aged rats are more vulnerable and less able to prevent oxidative changes that occur in response to acute heat exposure.
authors.affiliation: Faculty of Natural Sciences and Mathematics, Institute of Biology, "Sts, Cyril and Methodius" University, Skopje, Macedonia. mitkom@pmf.ukim.mk.
authors.forename: V
authors.initials: V
authors.lastname: Stojkovski
authors.forename: N
authors.initials: N
authors.lastname: Hadzi-Petrushev
authors.forename: V
authors.initials: V
authors.lastname: Ilieski
authors.forename: R
authors.initials: R
authors.lastname: Sopi
authors.forename: I
authors.initials: I
authors.lastname: Gjorgoski
authors.forename: D
authors.initials: D
authors.lastname: Mitrov
authors.forename: N
authors.initials: N
authors.lastname: Jankulovski
authors.forename: M
authors.initials: M
authors.lastname: Mladenov
mesh_terms: D000367:Age Factors; D000375:Aging / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D004798:Enzymes / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D018869:Heat-Shock Response / Q000187:drug effects*; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000201:enzymology; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000201:enzymology; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D024502:alpha-Tocopherol / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D004798:Enzymes; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D024502:alpha-Tocopherol