title: Determination of enterohemorrhagic Escherichia coli serotype O165:HNM infection in a hemolytic uremic syndrome patient with adenovirus seroype 41.
abstract: A 4-year-old girl who was positive for adenovirus according to a rapid immunochromatographic test conducted at a hospital, progressed to hemorrhagic diarrhea and hemolytic uremic syndrome (HUS). The presence of adenovirus serotype 41 (AdV-41) was confirmed by TaqMan real-time PCR and sequence analysis. However, most enteric viral infections cause mild to moderate diarrhea. In the present case, enterohemorrhagic Escherichia coli (EHEC) O165:HNM was isolated concomitantly with AdV-41. In addition, O165 antibody was specifically detected in patient sera. The EHEC isolate was positive for the virulence genes stx1, stx2a, eae type ε, ehxA, and norV. Therefore, we concluded that EHEC O165:HNM was the precise pathogen leading to HUS in this patient.
authors.affiliation: Health and Hygiene Division, Akita Prefectural Research Center for Public Health and Environment.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Konno
authors.forename: Jun
authors.initials: J
authors.lastname: Yatsuyanagi
authors.forename: Shiho
authors.initials: S
authors.lastname: Takahashi
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kumagai
authors.forename: Eriko
authors.initials: E
authors.lastname: Wada
authors.forename: Machiko
authors.initials: M
authors.lastname: Chiba
authors.forename: Shioko
authors.initials: S
authors.lastname: Saito
mesh_terms: D000257:Adenoviridae Infections / Q000150:complications* / Q000821:virology; D000260:Adenoviruses, Human / Q000145:classification / Q000302:isolation & purification*; D000907:Antibodies, Bacterial / Q000097:blood; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D054324:Enterohemorrhagic Escherichia coli / Q000145:classification / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000150:complications* / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics; D005260:Female; D006463:Hemolytic-Uremic Syndrome / Q000150:complications* / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction; D012703:Serotyping; D037521:Virulence Factors / Q000235:genetics
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D004279:DNA, Viral; D029968:Escherichia coli Proteins; D037521:Virulence Factors