title: Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis.
abstract: The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass and protein content was observed according to the 2v (5-1) fractional design. These factors were further evaluated using a central composite design and response surface methodology, and an empirical model for protein content was established and validated. The biomass of Yarrowia lipolytica NRRL YB-423 reached 19.5 ± 1.0 g/L in shaken flasks cultivation, with a protein content of 20.1 ± 0.6% (w/w).
authors.affiliation: Universidade Federal do Rio Grande, Escola de Química e Alimentos, Laboratório de Engenharia de Bioprocessos Rio Grande, RS , Brasil.
authors.forename: Elisane Odriosolla
authors.initials: EO
authors.lastname: Dos Santos
authors.forename: Mariano
authors.initials: M
authors.lastname: Michelon
authors.forename: Eliana Badiale
authors.initials: EB
authors.lastname: Furlong
authors.forename: Janaína Fernandes de Medeiros
authors.initials: JF
authors.lastname: Burkert
authors.forename: Susana Juliano
authors.initials: SJ
authors.lastname: Kalil
authors.forename: Carlos André Veiga
authors.initials: CA
authors.lastname: Burkert
keywords: Raw glycerol; Yarrowia lipolytica; single cell protein; yeast biomass
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