title: Development of analytical methods for the quantification of metabolites of lesogaberan in a MIST investigation.
abstract: Analytical methods were developed for the determination of six metabolites of lesogaberan to be used in quantitative determinations of metabolites according to the guidelines of Metabolites in Safety Testing. The γ-amino butyric acid type B receptor agonist lesogaberan and its metabolites are small polar molecules and hydrophilic interaction liquid chromatography was found to be a suitable separation mode. The samples were prepared using protein precipitation and negative electrospray ionization tandem mass spectrometry was used for detection. Initially, exploratory methods for six metabolites were set up for analysis of human plasma samples taken after repeated administration of a high oral dose of lesogaberan. The purpose was to establish which metabolites were present at concentrations significant for further investigation. Four of the six metabolites were then found at clearly detectable concentrations. The analytical methods for these four metabolites were further elaborated and then taken through a qualification procedure, which showed acceptable accuracy (86-114%), precision (<9%) and good linearity in the range 0.03-5 µmol/L. No interferences were seen from endogenous plasma components.
authors.affiliation: Regulatory Bioanalysis, Global IM, AstraZeneca R&D, Mölndal, Sweden.
authors.forename: Kristina
authors.initials: K
authors.lastname: Dunér
authors.forename: Pernilla
authors.initials: P
authors.lastname: Bottner
authors.forename: Anna-Karin
authors.initials: AK
authors.lastname: Norlén
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004285:Dogs; D004355:Drug Stability; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D013058:Mass Spectrometry; D010721:Phosphinic Acids / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D011437:Propylamines / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014481:United States; D014486:United States Food and Drug Administration
chemical_list: D010721:Phosphinic Acids; D011437:Propylamines; C545087:lesogaberan
keywords: HILIC; MIST; lesogaberan; metabolites