title: CD14 C-159T polymorphism and its association with chronic lung diseases: A pilot study on isocyanate exposed population of Central India.
abstract: BACKGROUND
  CD14 functions as a multifunctional receptor for bacterial cell wall components including endotoxin and lipopolysaccharide and is likely to influence the cytokine profile and subsequent immunoglobulin E production in response to antigen/allergen contact in allergic phenotypes.
  
  
  OBJECTIVE
  The present study was to investigate genetic polymorphism in CD14 gene - 159C/T, which may be one of the risk factor for increased prevalence of Chronic Lung Diseases in the Central India.
  
  
  METHODS
  Survivors of Methyl isocyanates toxicity in Bhopal still suffering from various respiratory ailments were examined.
  
  
  METHODS
  Polymerase chain reaction-restriction fragment length polymorphism was performed to determine the polymorphism of C-159T.
  
  
  RESULTS
  The genotype and allelic frequencies were in Hardy-Weinberg's equilibrium. Prevalence of CC, CT, and TT were 5.5%, 22.2% and 9.25% respectively in asthmatics; 16.6%, 20.3% and 5.5% respectively in chronic obstructive pulmonary disease (COPD) patients and 5.5%, 14.8% and 1.85 respectively among interstitial lung disorder (ILD) patients; whereas the control cohort with no methyl isocyanate exposure displayed (CC, CT, and TT) cytosine, thymine as 2%, 1.6% and 2% respectively. Increased risk of Asthma among those carrying TT genotype and T allele (odds ratio [OR] =2.61 and 2.02 respectively).
  
  
  CONCLUSIONS
  COPD risk significantly found among those with CC genotype and C allele (OR = 2.81 and 1.50 respectively), whereas ILD risk found significantly among CT genotype and C allele (OR = 1.75 and 1.40 respectively). Therefore, single nucleotide polymorphism (SNP) C-159T polymorphism in CD14 gene might be a risk factor for development of CLD in this population.
authors.affiliation: Department of Research, Bhopal Memorial Hospital and Research Center, Madhya Pradesh, India.
authors.forename: Protiti
authors.initials: P
authors.lastname: Bose
authors.forename: Rashmi
authors.initials: R
authors.lastname: Bathri
authors.forename: Sajal
authors.initials: S
authors.lastname: De
authors.forename: K K
authors.initials: KK
authors.lastname: Maudar
keywords: Asthma; Bhopal; CD14; chronic obstructive methyl isocyanate; pulmonary disease
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