title: Activity of disinfectants against foodborne pathogens in suspension and adhered to stainless steel surfaces.
abstract: The purpose of this study was to investigate and compare the efficacy of various disinfectants on planktonic cells and biofilm cells of Listeria monocytogenes, Staphylococcus aureus and Escherichia coli. Numbers of viable biofilm cells decreased after treatment with all tested disinfectants (iodine, biguanide, quaternary ammonium compounds, peracetic acid and sodium hypochlorite). Sodium hypochlorite was the most effective disinfectant against biofilm cells, while biguanide was the least effective. Scanning electron microscopy observations revealed that cells adhered on stainless steel surface after treatment with the disinfectants. No viable planktonic cells were observed after treatment with the same disinfectants. Based on our findings, we concluded that biofilm cells might be more resistant to disinfectants than plancktonic cells.
authors.affiliation: Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas , Araraquara, Sao Paulo , Brasil.
authors.forename: Tatiane Karen
authors.initials: TK
authors.lastname: Cabeça
authors.forename: Antonio Carlos
authors.initials: AC
authors.lastname: Pizzolitto
authors.forename: Elisabeth Loshchagin
authors.initials: EL
authors.lastname: Pizzolitto
keywords: biofilm cells; disinfectants; plancktonic cells
references.citation: Trends Microbiol. 2001 Jan;9(1):34-9
references.pmid: 11166241
references.citation: J Appl Microbiol. 2001 Aug;91(2):248-54
references.pmid: 11473589
references.citation: J Food Prot. 1984 Oct;47(10):756-759
references.pmid: 30934513
references.citation: Appl Environ Microbiol. 2001 May;67(5):2319-25
references.pmid: 11319118
references.citation: J Food Prot. 1990 Jul;53(7):550-554
references.pmid: 31018356
references.citation: J Food Prot. 2002 Jul;65(7):1117-21
references.pmid: 12117244
references.citation: Biol Pharm Bull. 2003 May;26(5):671-4
references.pmid: 12736510
references.citation: J Dairy Sci. 1998 Oct;81(10):2760-4
references.pmid: 9812281
references.citation: Emerg Infect Dis. 1999 Sep-Oct;5(5):607-25
references.pmid: 10511517
references.citation: J Food Prot. 1998 Jul;61(7):833-8
references.pmid: 9678165
references.citation: Antimicrob Agents Chemother. 2002 May;46(5):1469-74
references.pmid: 11959584
references.citation: J Bacteriol. 2000 Aug;182(15):4158-64
references.pmid: 10894722
references.citation: Appl Environ Microbiol. 2003 Dec;69(12):7188-93
references.pmid: 14660365
references.citation: Braz J Microbiol. 2010 Jan;41(1):97-106
references.pmid: 24031469
references.citation: Appl Environ Microbiol. 1988 Mar;54(3):649-54
references.pmid: 3288119
references.citation: J Appl Microbiol. 1999 Apr;86(4):576-82
references.pmid: 10212403
references.citation: J Dairy Sci. 1998 Oct;81(10):2765-70
references.pmid: 9812282
references.citation: Emerg Infect Dis. 1997 Oct-Dec;3(4):425-34
references.pmid: 9366593
references.citation: Compr Rev Food Sci Food Saf. 2003 Jan;2(1):22-32
references.pmid: 33451238
references.citation: Appl Environ Microbiol. 1999 Sep;65(9):4285-7
references.pmid: 10473455
references.citation: J Appl Microbiol. 2001 Jun;90(6):1000-5
references.pmid: 11412331
references.citation: Int J Antimicrob Agents. 1999 May;11(3-4):217-21; discussion 237-9
references.pmid: 10394973