title: Identification of Mamu-DPA1, Mamu-DQA1, and Mamu-DRA alleles in a cohort of Chinese rhesus macaques.
abstract: Rhesus macaques have long been used as animal models for various human diseases; the susceptibility and/or resistance to some of these diseases are related to the major histocompatibility complex (MHC). To gain insight into the MHC background and to facilitate the experimental use of Chinese rhesus macaques, Mamu-DPA1, Mamu-DQA1, and Mamu-DRA alleles were investigated in 30 Chinese rhesus macaques by gene cloning and sequencing. A total of 14 Mamu-DPA1, 17 Mamu-DQA1, and 9 Mamu-DRA alleles were identified in this study. Of these alleles, 22 novel sequences have not been documented in earlier studies, including nine Mamu-DPA1, ten Mamu-DQA1, and three Mamu-DRA alleles. Interestingly, like Mafa-DQA1 and Mafa-DPA1, more than two Mamu-DQA1 and Mamu-DPA1 alleles were detected in one animal in this study, which suggested that they might represent gene duplication. If our findings can be validated by other studies, it will further increase the number of known Mamu-DPA1 and Mamu-DQA1 polymorphisms. Our data also indicated significant differences in MHC class II allele distribution among the Chinese rhesus macaques, Vietnamese cynomolgus macaques, and the previously reported rhesus macaques, which were mostly of Indian origin. This information will not only promote the understanding of Chinese rhesus macaque MHC diversity and polymorphism but will also facilitate the use of Chinese rhesus macaques in studies of human disease.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Deng
authors.forename: Huiling
authors.initials: H
authors.lastname: Zhang
authors.forename: Ruirui
authors.initials: R
authors.lastname: Xiang
authors.forename: Zhenwu
authors.initials: Z
authors.lastname: Zhang
authors.forename: Fei
authors.initials: F
authors.lastname: Ling
authors.forename: Min
authors.initials: M
authors.lastname: Zhuo
authors.forename: Hongli
authors.initials: H
authors.lastname: Du
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Wang
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D002681:China; D027963:Chloride-Bicarbonate Antiporters / Q000235:genetics / Q000276:immunology; D005787:Gene Frequency; D005802:Genes, MHC Class II / Q000235:genetics*; D006239:Haplotypes; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008253:Macaca mulatta / Q000235:genetics / Q000276:immunology*; D010802:Phylogeny; D000076988:Sulfate Transporters
chemical_list: D027963:Chloride-Bicarbonate Antiporters; D000949:Histocompatibility Antigens Class II; C092258:Mamu-DQA1 protein, Macaca mulatta; C081011:SLC26A3 protein, human; D000076988:Sulfate Transporters
references.citation: Immunogenetics. 2003 Nov;55(8):540-51
references.pmid: 14566434
references.citation: Tissue Antigens. 2011 Aug;78(2):139-42
references.pmid: 21644929
references.citation: J Virol. 2008 Jan;82(2):859-70
references.pmid: 17989178
references.citation: Hum Immunol. 2012 May;73(5):547-53
references.pmid: 22365968
references.citation: Nat Biotechnol. 2011 Oct 16;29(11):1019-23
references.pmid: 22002653
references.citation: Hum Immunol. 1995 Feb;42(2):184-7
references.pmid: 7744622
references.citation: J Immunol. 2004 May 15;172(10):6152-7
references.pmid: 15128802
references.citation: Immunogenetics. 2007 Jun;59(6):449-62
references.pmid: 17384942
references.citation: Am J Phys Anthropol. 2001 Dec;116(4):296-301
references.pmid: 11745081
references.citation: Immunogenetics. 2013 Sep;65(9):667-74
references.pmid: 23793105
references.citation: Immunogenetics. 2011 Sep;63(9):611-8
references.pmid: 21614582
references.citation: Immunogenetics. 2011 Mar;63(3):155-66
references.pmid: 21132285
references.citation: Immunogenetics. 2010 Mar;62(3):123-36
references.pmid: 20094710
references.citation: Hum Immunol. 2008 Aug;69(8):513-21
references.pmid: 18582516