title: Efficacy of peracetic acid in rapid disinfection of Resilon and gutta-percha cones compared with sodium hypochlorite, chlorhexidine, and povidone-iodine.
abstract: BACKGROUND
  The aim of this investigation was to compare the effectiveness of 3% sodium hypochlorite (NaOCl), 2% chlorhexidine, 1% peracetic acid, and 10% povidone-iodine in the rapid disinfection of Resilon (Pentron Clinical Technologies, LLC, Wallingford, CT) and gutta-percha cones contaminated with Enterococcus faecalis and Bacillus subtilis.
  
  
  METHODS
  Two hundred fifty-six samples consisting of 128 gutta-percha cones and 128 Resilon cones were used in this study. The materials were tested for disinfection according to the type of solution (3% NaOCl, 2% chlorhexidine, 1% peracetic acid, or 10% povidone-iodine), the time of exposure to each solution (1 or 5 minutes), and the type of microorganisms (E. faecalis or B. subtilis). Subsequent to the disinfection, samples were placed in test tubes containing 10 mL Mueller-Hinton broth and incubated at 37°C for 7 days. All test tubes were observed at 24-hour intervals and visually checked for turbidity, signifying microbial growth.
  
  
  RESULTS
  In this study, 1% peracetic acid showed the best results for both 1 minute and 5 minutes of disinfection, 2% chlorhexidine showed the second best results although it was statistically at par with peracetic acid, and 3% hypochlorite ranked third in disinfection; this was statistically significant when compared with peracetic acid and chlorhexidine. Disinfection by povidone-iodine was the least within all the groups for both contact times although disinfection for 5 minutes showed better results than disinfection for 1 minute for gutta-percha.
  
  
  CONCLUSIONS
  The outcome of this study confirmed the efficacy of 1% peracetic acid and 2% chlorhexidine in the rapid disinfection of both Resilon and gutta-percha.
authors.affiliation: Department of Conservative Dentistry and Endodontics, Sri Ramakrishna Dental College and Hospital, Peelamedu, Coimbatore, Tamil Nadu, India. Electronic address: subha_dr2003@yahoo.com.
authors.forename: N
authors.initials: N
authors.lastname: Subha
authors.forename: V
authors.initials: V
authors.lastname: Prabhakar
authors.forename: Minu
authors.initials: M
authors.lastname: Koshy
authors.forename: K
authors.initials: K
authors.lastname: Abinaya
authors.forename: M
authors.initials: M
authors.lastname: Prabu
authors.forename: Lavanya
authors.initials: L
authors.lastname: Thangavelu
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects; D002710:Chlorhexidine / Q000494:pharmacology*; D004202:Disinfectants / Q000494:pharmacology*; D004203:Disinfection / Q000379:methods; D004340:Drug Contamination / Q000517:prevention & control; D013293:Enterococcus faecalis / Q000187:drug effects; D006171:Gutta-Percha / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D009391:Nephelometry and Turbidimetry; D010463:Peracetic Acid / Q000494:pharmacology*; D011206:Povidone-Iodine / Q000494:pharmacology*; D012387:Root Canal Filling Materials / Q000737:chemistry*; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors
chemical_list: D004202:Disinfectants; D012387:Root Canal Filling Materials; C486892:resilon sealer; D011206:Povidone-Iodine; D006171:Gutta-Percha; D012973:Sodium Hypochlorite; D010463:Peracetic Acid; D002710:Chlorhexidine
keywords: Bacillus subtilis; Enterococcus faecalis; Resilon; contact time; gutta-percha; peracetic acid; rapid disinfection