title: Cohnella lupini sp. nov., an endophytic bacterium isolated from root nodules of Lupinus albus.
abstract: A bacterial strain designated RLAHU4B(T) was isolated from root nodules of Lupinus albus in León (Spain). The 16S rRNA gene sequence of this strain showed similarities lower than 97 % with respect to species of the genus Cohnella. The strain was a Gram-variable, sporulating rod, motile by means of peritrichous flagella, and facultatively anaerobic. It was positive for oxidase, catalase and β-galactosidase production but negative for urease, amylase and gelatinase. Strain RLAHU4B(T) grew in the presence of 5 % NaCl. MK-7 was the predominant menaquinone and meso-diaminopimelic acid was present in the peptidoglycan. anteiso-C15 : 0, iso-C16 : 0, iso-C15 : 0 and C16 : 0 were the major fatty acids. Major polar lipids of strain RLAHU4B(T) were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unknown phospholipids, two unknown aminophospholipids and one unknown lipid. The DNA G+C content was 57.8 mol%. Strain RLAHU4B(T) presented phenotypic differences from all recognized species of the genus Cohnella. The phylogenetic, chemotaxonomic and phenotypic data indicated that strain RLAHU4B(T) belongs to a novel species of the genus Cohnella, for which the name Cohnella lupini sp. nov. is proposed, with strain RLAHU4B(T) ( = LMG 27416(T) = CECT 8236(T)) as the type strain.
authors.affiliation: Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
authors.forename: José David
authors.initials: JD
authors.lastname: Flores-Félix
authors.affiliation: Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
authors.forename: Lorena
authors.initials: L
authors.lastname: Carro
authors.affiliation: Unidad Asociada Grupo de Interacción Planta-Microorganismo Universidad de Salamanca-IRNASA-CSIC, Salamanca, Spain.|Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), Salamanca, Spain.
authors.forename: Martha-Helena
authors.initials: MH
authors.lastname: Ramírez-Bahena
authors.affiliation: Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Tejedor
authors.affiliation: Unidad Asociada Grupo de Interacción Planta-Microorganismo Universidad de Salamanca-IRNASA-CSIC, Salamanca, Spain.|Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), Salamanca, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Igual
authors.affiliation: Unidad Asociada Grupo de Interacción Planta-Microorganismo Universidad de Salamanca-IRNASA-CSIC, Salamanca, Spain.|Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), Salamanca, Spain.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Peix
authors.affiliation: Unidad Asociada Grupo de Interacción Planta-Microorganismo Universidad de Salamanca-IRNASA-CSIC, Salamanca, Spain.|Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
authors.forename: Encarna
authors.initials: E
authors.lastname: Velázquez
mesh_terms: D056449:Bacillales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D060026:Endophytes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry; D031297:Lupinus / Q000382:microbiology*; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013030:Spain; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7