title: Cloning and characterization of a putative R2R3 MYB transcriptional repressor of the rosmarinic acid biosynthetic pathway from Salvia miltiorrhiza.
abstract: Salvia miltiorrhiza Bunge is one of the most renowned traditional medicinal plants in China. Phenolic acids that are derived from the rosmarinic acid pathway, such as rosmarinic acid and salvianolic acid B, are important bioactive components in S. miltiorrhiza. Accumulations of these compounds have been reported to be induced by various elicitors, while little is known about transcription factors that function in their biosynthetic pathways. We cloned a subgroup 4 R2R3 MYB transcription factor gene (SmMYB39) from S. miltiorrhiza and characterized its roles through overexpression and RNAi-mediated silencing. As the results showed, the content of 4-coumaric acid, rosmarinic acid, salvianolic acid B, salvianolic acid A and total phenolics was dramatically decreased in SmMYB39-overexpressing S. miltiorrhiza lines while being enhanced by folds in SmMYB39-RNAi lines. Quantitative real-time PCR and enzyme activities analyses showed that SmMYB39 negatively regulated transcripts and enzyme activities of 4-hydroxylase (C4H) and tyrosine aminotransferase (TAT). These data suggest that SmMYB39 is involved in regulation of rosmarinic acid pathway and acts as a repressor through suppressing transcripts of key enzyme genes.
authors.affiliation: College of Life Sciences, Northwest A & F University, Yangling, People's Republic of China.
authors.forename: Shuncang
authors.initials: S
authors.lastname: Zhang
authors.forename: Pengda
authors.initials: P
authors.lastname: Ma
authors.forename: Dongfeng
authors.initials: D
authors.lastname: Yang
authors.forename: Wenjing
authors.initials: W
authors.lastname: Li
authors.forename: Zongsuo
authors.initials: Z
authors.lastname: Liang
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Fenghua
authors.initials: F
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D053898:Biosynthetic Pathways; D002934:Cinnamates / Q000378:metabolism*; D019976:Cloning, Organism; D053630:Depsides / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000097123:Rosmarinic Acid
chemical_list: D002934:Cinnamates; D053630:Depsides; D010940:Plant Proteins; D014157:Transcription Factors; D000097123:Rosmarinic Acid
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