title: Prevalence of newcastle disease virus in broiler chickens (Gallus gallus) in Brazil.
abstract: This study was carried out during 2002/2003, aiming to determine the prevalence of virulent Newcastle disease virus strains (NDV) in Brazilian commercial poultry farms. Clinical samples were obtained from the Southeastern, Southern and Central-Western regions, which comprise the main area of the Brazilian poultry production. Serum samples and tracheal and cloacal swabs of 23,745 broiler chickens from 1,583 flocks, including both vaccinated chickens and those with no vaccination information, were tested for NDV using a diagnostic ELISA kit. The seropositivity was 39.1%, and the isolation percentage by flock varied from 1.0 to 7.6%, and by region from 6.5 to 58.4%. Higher isolation rates (74.3-83.3%) were obtained after three passages in embryonated chicken eggs. All isolates preliminarily identified as NDV were characterized as nonpathogenic strains, as their Intracerebral Pathogenicity Index (ICPI) was below 0.7. Based on results of this study, Brazil can claim a virulent NDV-free status for commercial flocks.
authors.affiliation: Laboratório Nacional Agropecuário, Ministério da Agricultura, Pecuária e Abastecimento , Campinas, SP , Brasil ; Faculdade de Ciências Médicas, Universidade Estadual de Campinas , Campinas, SP , Brasil.
authors.forename: M A
authors.initials: MA
authors.lastname: Orsi
authors.forename: L
authors.initials: L
authors.lastname: Doretto
authors.forename: S C A
authors.initials: SC
authors.lastname: Camillo
authors.forename: D
authors.initials: D
authors.lastname: Reischak
authors.forename: S A M
authors.initials: SA
authors.lastname: Ribeiro
authors.forename: A
authors.initials: A
authors.lastname: Ramazzoti
authors.forename: A O
authors.initials: AO
authors.lastname: Mendonça
authors.forename: F R
authors.initials: FR
authors.lastname: Spilki
authors.forename: M G
authors.initials: MG
authors.lastname: Buzinaro
authors.forename: H L
authors.initials: HL
authors.lastname: Ferreira
authors.forename: C W
authors.initials: CW
authors.lastname: Arns
keywords: NDV-free status; Newcastle Disease Virus; biological characterization; broiler chickens; pathogenicity
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