title: Enantioseparation and determination of the chiral fungicide furametpyr enantiomers in rice, soil, and water by high-performance liquid chromatography.
abstract: The chiral fungicide furametpyr is widely used in the rice field to control rice sheath blight; however, furametpyr enantiomers are treated as just one compound in traditional achiral analysis, which gives only partial information. An effective chiral analytical method was developed for the resolution and determination of the fungicide furametpyr enantiomers in rice, soil, and water samples. Furametpyr enantiomers were excellently separated and determined on a Chiralpak AD-H column with n-hexane/ethanol (90:10, v/v) as mobile phase at a flow rate of 0.8 mL min(-1) with UV detection at 220 nm. The resolution was up to 8.85. The first eluted enantiomer was (+)-furametpyr and the second eluted one was (-)-furametpyr. The effects of mobile-phase composition and column temperature on the enantioseparation were evaluated. The method was validated for linearity, repeatability, accuracy, limit of detection (LOD), and limit of quantification LOQ. LOD was 2.0 µg kg(-1) in water, 0.02 mg kg(-1) in soil, and 0.07 mg kg(-1) in rice with an LOQ of 6.7 µg kg(-1) in water, 0.07 mg kg(-1) in soil, and 0.23 mg kg(-1) in rice. The average recoveries of the pesticide in all matrices ranged from 73.1 to 101.8% for all fortification levels. The precision values associated with the analytical method, expressed as relative standard deviation (RSD) values, were below 14.0% in all matrices. The methodology was successfully applied for the enantioselective analysis of furametpyr enantiomers in real samples.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P. R. China.
authors.forename: Fengshou
authors.initials: F
authors.lastname: Dong
authors.forename: Xiu
authors.initials: X
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.forename: Xingang
authors.initials: X
authors.lastname: Liu
authors.forename: Zenglong
authors.initials: Z
authors.lastname: Chen
authors.forename: Yuanbo
authors.initials: Y
authors.lastname: Li
authors.forename: Hongjun
authors.initials: H
authors.lastname: Zhang
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D001572:Benzofurans / Q000032:analysis* / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D005659:Fungicides, Industrial / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D015394:Molecular Structure; D012275:Oryza / Q000737:chemistry*; D011720:Pyrazoles / Q000032:analysis* / Q000737:chemistry; D012987:Soil / Q000737:chemistry*; D013237:Stereoisomerism; D013997:Time Factors; D014867:Water / Q000737:chemistry*
chemical_list: D001572:Benzofurans; D005659:Fungicides, Industrial; D011720:Pyrazoles; D012987:Soil; D014867:Water; C417978:furametpyr
keywords: chiral fungicides; furametpyr; residue; rice; soil; water