title: Dioxin pollution disrupts reproduction in male Japanese field mice.
abstract: Dioxins cause various adverse effects in animals including teratogenesis, induction of drug metabolizing enzymes, tumor promotion, and endocrine disruption. Above all, endocrine disruption is known to disturb reproduction in adult animals and may, also seriously impact their offspring. However, most previous studies have quantified the species-specific accumulation of dioxins, whereas few studies have addressed the physiological impacts of dioxins on wildlife, such as reduced reproductive function. Here we clarify an effect of endocrine disruption caused by dioxins on the Japanese field mouse, Apodemus speciosus. Japanese field mice collected from various sites polluted with dioxins accumulated high concentrations of dioxins in their livers. Some dioxin congeners, especially, 1,2,3,4,6,7,8-heptachlorodibenzo-p-dioxin, 3,3',4,4',5-pentachloro biphenyl, 1,2,3,4,6,7,8-heptachlorodibenzofuran, and octachlorodibenzo-p-dioxin, which showed high biota-soil accumulation factors, contributed to concentration of dioxins in mouse livers with an increase of accumulation of total dioxins. As for physiological effects on the Japanese field mouse, high levels of cytochrome P450 1A1 (CYP1A1) mRNA, a drug metabolizing enzyme induced by dioxins, were found in the livers of mice captured at polluted sites. Furthermore, at such sites polluted with dioxins, increased CYP1A1 expression coincided with reduced numbers of active spermatozoa in mice. Thus, disruption in gametogenesis observed in these mice suggests that dioxins not only negatively impact reproduction among Japanese field mice, but might also act as a kind of selection pressure in a chemically polluted environment.
authors.affiliation: Department of Environmental Science and Technology, Graduate School of Science and Technology, Niigata University, 8050, Ikarashi 2-no-cho, Nishi-ku, Niigata City, 950-2181, Japan, h_ishiniwa@yahoo.co.jp.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Ishiniwa
authors.forename: Mizuki
authors.initials: M
authors.lastname: Sakai
authors.forename: Shimon
authors.initials: S
authors.lastname: Tohma
authors.forename: Hidenori
authors.initials: H
authors.lastname: Matsuki
authors.forename: Yukio
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Hideo
authors.initials: H
authors.lastname: Kajiwara
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Sekijima
mesh_terms: D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004147:Dioxins / Q000032:analysis / Q000633:toxicity*; D008099:Liver / Q000737:chemistry*; D008297:Male; D051379:Mice; D051189:Murinae; D012098:Reproduction / Q000187:drug effects*; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000032:analysis / Q000633:toxicity*; D013076:Sperm Count; D013091:Spermatogenesis / Q000187:drug effects
chemical_list: D004147:Dioxins; D012987:Soil; D012989:Soil Pollutants; D019363:Cytochrome P-450 CYP1A1
references.citation: Biochim Biophys Acta. 2003 Feb 17;1619(3):263-8
references.pmid: 12573486
references.citation: Environ Sci Technol. 2001 Feb 15;35(4):732-8
references.pmid: 11349285
references.citation: Nature. 2002 Jul 11;418(6894):174-7
references.pmid: 12110888
references.citation: Arch Environ Contam Toxicol. 2000 Oct;39(3):398-410
references.pmid: 10948292
references.citation: Biochem Biophys Res Commun. 1998 Dec 9;253(1):44-8
references.pmid: 9875217
references.citation: Chemosphere. 2003 Oct;53(4):347-62
references.pmid: 12946393
references.citation: Arch Toxicol. 2002 Mar;76(2):113-8
references.pmid: 11914781
references.citation: Sci Total Environ. 1999 Jun 1;230(1-3):145-207
references.pmid: 10466229
references.citation: J Toxicol Environ Health. 1991 Aug;33(4):455-520
references.pmid: 1875430
references.citation: J Evol Biol. 2005 May;18(3):557-67
references.pmid: 15842485
references.citation: BJU Int. 2008 Jan;101(2):250-5
references.pmid: 17868417
references.citation: Toxicol Sci. 2007 Jun;97(2):318-35
references.pmid: 17383972
references.citation: Nature. 2007 Mar 29;446(7135):562-6
references.pmid: 17392787
references.citation: Arch Toxicol. 1996;70(8):510-3
references.pmid: 8783816
references.citation: Annu Rev Pharmacol Toxicol. 1982;22:517-54
references.pmid: 6282188
references.citation: J Comp Pathol. 1988 Apr;98(3):287-311
references.pmid: 3134469
references.citation: Environ Health Perspect. 2011 May;119(5):713-8
references.pmid: 21262597
references.citation: Chem Res Toxicol. 2008 Jan;21(1):102-16
references.pmid: 18076143
references.citation: Arch Toxicol. 2003 May;77(5):280-4
references.pmid: 12734642
references.citation: Arch Environ Contam Toxicol. 1989 Sep;18(5):706-27
references.pmid: 2802675
references.citation: J Toxicol Environ Health. 1991 Aug;33(4):549-85
references.pmid: 1908526
references.citation: Chemosphere. 2001 Aug;44(4):873-85
references.pmid: 11482680
references.citation: Environ Sci Pollut Res Int. 1996 Sep;3(3):122-8
references.pmid: 24235049
references.citation: Arch Environ Contam Toxicol. 2002 Feb;42(2):244-55
references.pmid: 11815817
references.citation: Pharmacol Rev. 1994 Dec;46(4):483-549
references.pmid: 7899475
references.citation: Annu Rev Pharmacol Toxicol. 2003;43:309-34
references.pmid: 12540743
references.citation: Ecotoxicology. 2002 Apr;11(2):119-25
references.pmid: 11990768
references.citation: Mol Cell Endocrinol. 2004 Jun 30;221(1-2):87-96
references.pmid: 15223135
references.citation: Environ Toxicol Chem. 2002 Apr;21(4):794-806
references.pmid: 11951954
references.citation: Mar Pollut Bull. 2006 Sep;52(9):1066-76
references.pmid: 16540125
references.citation: Proc Natl Acad Sci U S A. 1996 Mar 19;93(6):2322-7
references.pmid: 8637871
references.citation: Chemosphere. 2003 Oct;53(4):337-45
references.pmid: 12946392
references.citation: Biomarkers. 2001;6(1):19-25
references.pmid: 23886053
references.citation: Bull Environ Contam Toxicol. 2005 Jul;75(1):15-22
references.pmid: 16228867
references.citation: Toxicol Sci. 2006 Oct;93(2):223-41
references.pmid: 16829543
references.citation: Sci Total Environ. 1998 Aug 12;219(1):53-81
references.pmid: 9770324
references.citation: J Biol Chem. 1994 Nov 4;269(44):27337-43
references.pmid: 7961644
references.citation: Arch Environ Contam Toxicol. 2000 May;38(4):509-21
references.pmid: 10787103
references.citation: Toxicol Sci. 2005 Dec;88(2):384-99
references.pmid: 16177242
references.citation: PLoS One. 2010 Mar 08;5(3):e9581
references.pmid: 20221392
references.citation: Environ Pollut. 2006 Apr;140(3):525-35
references.pmid: 16159690
references.citation: Chemosphere. 2003 Oct;53(4):315-24
references.pmid: 12946390
references.citation: Environ Sci Technol. 2005 Apr 15;39(8):2417-25
references.pmid: 15884330
references.citation: PLoS One. 2007 Jan 24;2(1):e170
references.pmid: 17245446
references.citation: Sci Total Environ. 2010 Jul 1;408(15):2995-3043
references.pmid: 19910021
references.citation: Chemosphere. 2003 Oct;53(4):427-36
references.pmid: 12946400
references.citation: Mutat Res. 2004 Aug 18;552(1-2):73-100
references.pmid: 15288543
references.citation: Environ Pollut. 2008 Jan;151(1):252-61
references.pmid: 17544187
references.citation: Sci Total Environ. 1996 Apr 5;182(1-3):93-103
references.pmid: 8854941