title: [Cloning and sequence characterization of replication region in plasmid pJTU112 from Micoromonospora sp. 40027].
abstract: Micoromonospora sp. 40027, the producer of fortimicin A, harbors two plasmids pJTU101 and pJTU112.
  
  
  OBJECTIVE
  Cloning and sequencing of replication region of pJTU12, analyzing replication region sequence of pJTU112.
  
  
  METHODS
  Different fragments of pJTU112 were cloned and introduced by conjugation into Micromonospora sp. LXH20. The replication region of pJTU112 was identified.
  
  
  RESULTS
  The replication region of pJTU112 was located in the 4.7 kb SacI-KpnI DNA fragment. DNA sequencing and analysis revealed that the 4.7 kb SacI-KpnI DNA fragment encoded five open reading frames. The pJTU112.1 and pJTU112.2 were related to plasmid conjugation, pJTU112.3, pJTU112.4 and pJTU112.5 were related to plasmid conjugation.
  
  
  CONCLUSIONS
  The replication region of pJTU112 was located in the 4.7kb SacI-KpnI DNA fragment.
authors.affiliation: Key Lab for Biotechnology of the State Ethnic Affairs Commission, College of Life Science, South-Central University for Nationalities, Wuhan 430074, China. gengxumei@163.com
authors.forename: Xumei
authors.initials: X
authors.lastname: Geng
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Yang
authors.forename: Jia
authors.initials: J
authors.lastname: Guo
authors.forename: Guifa
authors.initials: G
authors.lastname: Zhai
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D008847:Micromonospora / Q000235:genetics*; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics*; D018741:Replication Origin; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins