title: Highly sensitive and rapid assay of substance P and streptolysin O in human serum using immuno-liposomes and gramicidin channels.
abstract: A highly sensitive and rapid method for the determination of substance P (SP) and streptolysin O (SLO) in human serum is described. The assay is based on enriching the analyte by agglutination/precipitation of immuno-liposomes and enhancing the fluorescence intensity by gramicidin A channels. A mixture of the immuno-liposomes encapsulating a pH-sensitive fluorescence dye BCECF, gramicidin A and a given concentration of SP (or SLO) is preincubated in a solution and captured on anti-SP (or anti-SLO)-modified cover slips, followed by measuring fluorescence images after removing excess liposomes. The method allowed quantifying SP and SLO in the range from sub-pg mL(-1) to pg mL(-1), with detection limits of 0.32 pg mL(-1) and 8 fg mL(-1), respectively. The present method could determine SP and SLO in 50-125 times diluted human serum without any extraction steps. The assay can be completed within 60 min.
authors.affiliation: Department of Chemistry, College of Humanities and Sciences, Nihon University.
authors.forename: Misato
authors.initials: M
authors.lastname: Sakamoto
authors.forename: Atsushi
authors.initials: A
authors.lastname: Shoji
authors.forename: Masao
authors.initials: M
authors.lastname: Sugawara
mesh_terms: D001426:Bacterial Proteins / Q000097:blood; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D006096:Gramicidin / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay; D008081:Liposomes; D013301:Streptolysins / Q000097:blood*; D013373:Substance P / Q000097:blood*
chemical_list: D001426:Bacterial Proteins; D005456:Fluorescent Dyes; D008081:Liposomes; D013301:Streptolysins; C025964:streptolysin O; D006096:Gramicidin; D013373:Substance P