title: Levels of key enzymes of methionine-homocysteine metabolism in preeclampsia.
abstract: OBJECTIVE
  To evaluate the role of key enzymes in the methionine-homocysteine metabolism (MHM) in the physiopathology of preeclampsia (PE).
  
  
  METHODS
  Plasma and placenta from pregnant women (32 controls and 16 PE patients) were analyzed after informed consent. Protein was quantified by western blot. RNA was obtained with RNA purification kit and was quantified by reverse transcritase followed by real-time PCR (RT-qPCR). Identification of the C677T and A1298C methylenetetrahydrofolate reductase (MTHFR) single-nucleotide polymorphisms (SNPs) and A2756G methionine synthase (MTR) SNP was performed using PCR followed by a high-resolution melting (HRM) analysis. S-adenosyl methionine (SAM) and S-adenosyl homocysteine (SAH) were measured in plasma using high-performance liquid chromatography-tandem mass spectrometry (HPLC/MS/MS). The SNP association analysis was carried out using Fisher's exact test. Statistical analysis was performed using a Mann-Whitney test.
  
  
  RESULTS
  RNA expression of MTHFR and MTR was significantly higher in patients with PE as compared with controls. Protein, SAM, and SAH levels showed no significant difference between preeclamptic patients and controls. No statistical differences between controls and PE patients were observed with the different SNPs studied.
  
  
  CONCLUSIONS
  The RNA expression of MTHFR and MTR is elevated in placentas of PE patients, highlighting a potential compensation mechanism of the methionine-homocysteine metabolism in the physiopathology of this disease.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Universidad de los Andes, San Carlos de Apoquindo, 7620001 Santiago, Chile.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Pérez-Sepúlveda
authors.forename: Pedro P
authors.initials: PP
authors.lastname: España-Perrot
authors.forename: Ximena B
authors.initials: XB
authors.lastname: Fernández
authors.forename: Verónica
authors.initials: V
authors.lastname: Ahumada
authors.forename: Vicente
authors.initials: V
authors.lastname: Bustos
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Arraztoa
authors.forename: Aneta
authors.initials: A
authors.lastname: Dobierzewska
authors.forename: Horacio
authors.initials: H
authors.lastname: Figueroa-Diesel
authors.forename: Gregory E
authors.initials: GE
authors.lastname: Rice
authors.forename: Sebastián E
authors.initials: SE
authors.lastname: Illanes
mesh_terms: D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / Q000096:biosynthesis / Q000235:genetics*; D000328:Adult; D005260:Female; D005786:Gene Expression Regulation; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D008715:Methionine / Q000097:blood*; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000096:biosynthesis / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000097:blood / Q000201:enzymology*; D011247:Pregnancy; D012313:RNA / Q000235:genetics; D012435:S-Adenosylhomocysteine / Q000097:blood; D012436:S-Adenosylmethionine / Q000097:blood
chemical_list: D006710:Homocysteine; D012313:RNA; D012436:S-Adenosylmethionine; D012435:S-Adenosylhomocysteine; D008715:Methionine; C573423:MTHFR protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2); D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase; C585801:MTR protein, human
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