title: Analysis of cytotoxic T-lymphocyte-associated antigen-4 and MMP-9 genes' methylation and their expression profiles with risk of non-alcoholic fatty liver disease.
abstract: OBJECTIVE
  To investigate the effect of promoter methylation of cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) gene and matrix metalloproteinases (MMPs) on the risk of non-alcoholic fatty liver disease (NAFLD).
  
  
  METHODS
  CTLA-4 and MMP-9 promoter methylation were investigated using a methylation-specific polymerase chain reaction (MS-PCR) in blood samples taken from 80 NAFLD individuals and 95 healthy controls. The expression levels of CTLA-4 and MMP-9 were also assessed in 10 blood and 9 liver tissues mRNA samples from NAFLD patients. These cases were compared to the blood (n = 10) samples of healthy controls with real-time quantitative reverse transcriptase PCR.
  
  
  RESULTS
  No significant relationship was found for methylation of CTLA-4 and MMP-9 between cases and controls. The relative expression of CTLA-4 and MMP-9 mRNA in NAFLD was not significantly different compared to healthy control samples.
  
  
  CONCLUSIONS
  For the first time, our outcomes indicate that the methylation status of CTLA-4 and MMP-9 genes has no significant function on the process of NAFLD.
authors.affiliation: Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran.
authors.forename: Dor Mohammad Kordi
authors.initials: DM
authors.lastname: Tamandani
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hashemi
authors.forename: Sara
authors.initials: S
authors.lastname: Shafiepour
keywords: Cytotoxic T-lymphocyte-associated antigen-4; expression; gene; matrix metalloproteinases-9; methylation; non-alcoholic fatty liver disease
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