title: Induction of amino acid transporters expression by endurance exercise in rat skeletal muscle.
abstract: We here investigated whether an acute bout of endurance exercise would induce the expression of amino acid transporters that regulate leucine transport across plasma and lysosomal membranes in rat skeletal muscle. Rats ran on a motor-driven treadmill at a speed of 28 m/min for 90 min. Immediately after the exercise, we observed that expression of mRNAs encoding L-type amino acid transporter 1 (LAT1) and CD98 was induced in the gastrocnemius, soleus, and extensor digitorum longus (EDL) muscles. Sodium-coupled neutral amino acid transporter 2 (SNAT2) mRNA was also induced by the exercise in those three muscles. Expression of proton-assisted amino acid transporter 1 (PAT1) mRNA was slightly but not significantly induced by a single bout of exercise in soleus and EDL muscles. Exercise-induced mRNA expression of these amino acid transporters appeared to be attenuated by repeated bouts of the exercise. These results suggested that the expression of amino acid transporters for leucine may be induced in response to an increase in the requirement for this amino acid in the cells of working skeletal muscles.
authors.affiliation: Department of Nutrition, Shigakkan University, 55 Nakoyama, Yokone-Machi, Ohbu 474-8651, Japan. Electronic address: tamuraka@sgk.ac.jp.
authors.forename: Taro
authors.initials: T
authors.lastname: Murakami
authors.forename: Mariko
authors.initials: M
authors.lastname: Yoshinaga
mesh_terms: D026924:Amino Acid Transport System A; D026905:Amino Acid Transport Systems / Q000235:genetics* / Q000378:metabolism; D026921:Amino Acid Transport Systems, Neutral / Q000235:genetics / Q000378:metabolism; D000818:Animals; D027261:Fusion Regulatory Protein-1 / Q000235:genetics / Q000378:metabolism; D027283:Large Neutral Amino Acid-Transporter 1 / Q000235:genetics / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D010807:Physical Endurance / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D027981:Symporters / Q000235:genetics / Q000378:metabolism
chemical_list: D026924:Amino Acid Transport System A; D026905:Amino Acid Transport Systems; D026921:Amino Acid Transport Systems, Neutral; D027261:Fusion Regulatory Protein-1; D027283:Large Neutral Amino Acid-Transporter 1; D012333:RNA, Messenger; C493537:Slc36a1 protein, rat; C412568:Slc38a2 protein, rat; D027981:Symporters
keywords: CD98; Exercise; Muscle; Proton-assisted amino acid transporter 1; Sodium-coupled neutral amino acid transporter 2; l-Type amino acid transporter 1