title: Knockdown of CRM1 inhibits the nuclear export of p27(Kip1) phosphorylated at serine 10 and plays a role in the pathogenesis of epithelial ovarian cancer.
abstract: In a previous study, the nuclear export protein chromosomal region maintenance (CRM1) was correlated with p27(Kip1) in glioma. The aims of the present study were to investigate the expression of CRM1 and pSer10p27 and their functional roles in epithelial ovarian cancer (EOC) tissues. Using immunohistochemical analysis, CRM1 and pSer10p27 expression levels were shown to be associated with histologic stage and grade (P<0.05). High CRM1 and pSer10p27 expression levels were prognostic indicators of overall survival (P<0.05). Knockdown of CRM1 and pSer10p27 expression arrested cell cycle progression and inhibited the proliferation of SKOV3 cells both in vitro and in vivo. These data support the idea that pSer10p27 and CRM1 play cooperative roles in EOC.
authors.affiliation: Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200001, PR China; Department of Obstetrics and Gynecology, Wuxi Maternal and Child Health Hospital, Nanjing Medical University, Wuxi 214000, PR China.
authors.forename: You
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Nantong University Cancer Hospital, Nantong University, Nantong 226001, Jiangsu, PR China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Wuxi Maternal and Child Health Hospital, Nanjing Medical University, Wuxi 214000, PR China.
authors.forename: Jingying
authors.initials: J
authors.lastname: Xiang
authors.affiliation: Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200001, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Ji
authors.affiliation: Department of Obstetrics and Gynecology, Nantong University Cancer Hospital, Nantong University, Nantong 226001, Jiangsu, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Obstetrics and Gynecology, Nantong University Cancer Hospital, Nantong University, Nantong 226001, Jiangsu, PR China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Tang
authors.affiliation: Department of Obstetrics and Gynecology, Nantong University Cancer Hospital, Nantong University, Nantong 226001, Jiangsu, PR China.
authors.forename: Shuyun
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Obstetrics and Gynecology, Nantong University Cancer Hospital, Nantong University, Nantong 226001, Jiangsu, PR China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Xi
authors.affiliation: Department of Obstetrics and Gynecology, Nantong University Cancer Hospital, Nantong University, Nantong 226001, Jiangsu, PR China. Electronic address: liurong0513@sina.cn.
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200001, PR China. Electronic address: diwen163@163.com.
authors.forename: Wen
authors.initials: W
authors.lastname: Di
mesh_terms: D021581:Active Transport, Cell Nucleus; D000328:Adult; D000818:Animals; D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D028884:Karyopherins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D009368:Neoplasm Transplantation; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics* / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism; D010766:Phosphorylation; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics* / Q000378:metabolism; D012694:Serine / Q000737:chemistry; D000097606:Exportin 1 Protein
chemical_list: D050760:Cyclin-Dependent Kinase Inhibitor p27; D028884:Karyopherins; D018160:Receptors, Cytoplasmic and Nuclear; D012694:Serine; D000097606:Exportin 1 Protein; C436291:CDKN1B protein, human
keywords: Chromosomal region maintenance; Epithelial ovarian cancer; Phosphorylation; Proliferation; p27(Kip1)