title: Detection of SNP-containing human DNA sequences using a split sensor with a universal molecular beacon reporter.
abstract: Hybridization-based techniques have been extensively employed for the analysis of specific DNA/RNA sequences. Herein, we describe highly specific inexpensive smart hybridization-based sensor that takes advantage of a universal molecular beacon probe as a fluorescent reporter. The sensor has a straightforward design, and demonstrates improved selectivity and specificity of nucleic acid recognition. It is cost-efficient since it utilizes the same molecular beacon probe for the analysis of many nucleic acid sequences.
authors.affiliation: Department of Chemistry, University of Central Florida, Orlando, FL, USA.
authors.forename: Yulia V
authors.initials: YV
authors.lastname: Gerasimova
authors.forename: Jack
authors.initials: J
authors.lastname: Ballantyne
authors.forename: Dmitry M
authors.initials: DM
authors.lastname: Kolpashchikov
mesh_terms: D001483:Base Sequence; D053837:DNA Cleavage; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics / Q000302:isolation & purification; D005456:Fluorescent Dyes / Q000737:chemistry; D005819:Genetic Markers; D060005:Genotyping Techniques; D006801:Humans; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
chemical_list: D015342:DNA Probes; D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D005819:Genetic Markers