title: The POZ-ZF transcription factor Kaiso (ZBTB33) induces inflammation and progenitor cell differentiation in the murine intestine.
abstract: Since its discovery, several studies have implicated the POZ-ZF protein Kaiso in both developmental and tumorigenic processes. However, most of the information regarding Kaiso's function to date has been gleaned from studies in Xenopus laevis embryos and mammalian cultured cells. To examine Kaiso's role in a relevant, mammalian organ-specific context, we generated and characterized a Kaiso transgenic mouse expressing a murine Kaiso transgene under the control of the intestine-specific villin promoter. Kaiso transgenic mice were viable and fertile but pathological examination of the small intestine revealed distinct morphological changes. Kaiso transgenics (Kaiso(Tg/+)) exhibited a crypt expansion phenotype that was accompanied by increased differentiation of epithelial progenitor cells into secretory cell lineages; this was evidenced by increased cell populations expressing Goblet, Paneth and enteroendocrine markers. Paradoxically however, enhanced differentiation in Kaiso(Tg/+) was accompanied by reduced proliferation, a phenotype reminiscent of Notch inhibition. Indeed, expression of the Notch signalling target HES-1 was decreased in Kaiso(Tg/+) animals. Finally, our Kaiso transgenics exhibited several hallmarks of inflammation, including increased neutrophil infiltration and activation, villi fusion and crypt hyperplasia. Interestingly, the Kaiso binding partner and emerging anti-inflammatory mediator p120(ctn) is recruited to the nucleus in Kaiso(Tg/+) mice intestinal cells suggesting that Kaiso may elicit inflammation by antagonizing p120(ctn) function.
authors.affiliation: Department of Biology, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Roopali
authors.initials: R
authors.lastname: Chaudhary
authors.forename: Christina C
authors.initials: CC
authors.lastname: Pierre
authors.forename: Kyster
authors.initials: K
authors.lastname: Nanan
authors.forename: Daria
authors.initials: D
authors.lastname: Wojtal
authors.forename: Simona
authors.initials: S
authors.lastname: Morone
authors.forename: Christopher
authors.initials: C
authors.lastname: Pinelli
authors.forename: Geoffrey A
authors.initials: GA
authors.lastname: Wood
authors.forename: Sylvie
authors.initials: S
authors.lastname: Robine
authors.forename: Juliet M
authors.initials: JM
authors.lastname: Daniel
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D051177:Catenins / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D020895:Enterocytes / Q000378:metabolism / Q000473:pathology; D018703:Founder Effect; D005786:Gene Expression Regulation; D020397:Goblet Cells / Q000378:metabolism / Q000473:pathology; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D007249:Inflammation / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008822:Mice, Transgenic; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D020556:Neutrophil Infiltration / Q000235:genetics*; D019879:Paneth Cells / Q000378:metabolism / Q000473:pathology; D011401:Promoter Regions, Genetic; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013234:Stem Cells / Q000378:metabolism / Q000473:pathology; D000072056:Transcription Factor HES-1; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D019076:Transgenes; D000095002:Delta Catenin
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D051177:Catenins; D018398:Homeodomain Proteins; D008840:Microfilament Proteins; D051880:Receptors, Notch; D000072056:Transcription Factor HES-1; D014157:Transcription Factors; D000095002:Delta Catenin; C489734:Hes1 protein, mouse; C118822:Zbtb33 protein, mouse; C026615:villin; C000722349:Ctnnd1 protein, mouse
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