title: Optimization of exopolysaccharides production from a novel strain of Ganoderma lucidum CAU5501 in submerged culture.
abstract: This study aimed at optimizing the medium of a new Ganoderma lucidum strain CAU5501 to enhance the yield of exopolysaccharides (EPS) and mycelial growth. Firstly, the suitable level of glucose, magnesium, phosphate and C/N ratio was determined by single factor experiment. Subsequently, the optimum concentrations of these medium components were investigated using the orthogonal matrix method. The results indicated that the higher levels of EPS were correlated with the level of cell growth when glucose concentration was studied (data no show). The optimum medium for EPS yield was found to be 70 g/l glucose, 5 C/N ratio, 2.5 g/l KH2PO4, 0.75 g/l MgSO4·7H2O, and for mycelial growth was 50 g/l glucose, 5 C/N ratio, 1.5 g/l KH2PO4, 0.5 g/l MgSO4·7H2O. When cultivated in the obtained optimal media in 3 L shake flask, compared to the basal medium, the EPS yield increased markedly from 1.003 to 1.723 g/l, and the mycelium formation was also markedly improved from 2.028 to 7.235 g/l. Results obtained in this study are beneficial to further study for enhancing the production of Ganoderma lucidum polysaccharides in large scale commercialized production.
authors.affiliation: Laboratory of Feed Biotechnology, State Key Lab. of Animal Nutrition, College of Animal Science & Technology, China Agricultural University , No.2 Yuanmingyuan West Road, Haidian District, Beijing 100193 , China.
authors.forename: Baojing
authors.initials: B
authors.lastname: Yuan
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Chi
authors.forename: Rijun
authors.initials: R
authors.lastname: Zhang
keywords: Ganoderma lucidum; Optimization; exopolysaccharides; orthogonal matrix method; submerged culture
references.citation: Carcinogenesis. 2004 Jul;25(7):1177-83
references.pmid: 15205366
references.citation: J Biol Chem. 1967 Aug 25;242(16):3719-22
references.pmid: 5341484
references.citation: J Microbiol. 2006 Apr;44(2):233-42
references.pmid: 16728961
references.citation: Bioresour Technol. 2008 May;99(8):3187-94
references.pmid: 17624770
references.citation: J Cell Physiol. 2007 Aug;212(2):537-50
references.pmid: 17474083
references.citation: Braz J Microbiol. 2008 Apr;39(2):195-208
references.pmid: 24031202
references.citation: Prikl Biokhim Mikrobiol. 2005 Mar-Apr;41(2):194-9
references.pmid: 15859463
references.citation: RNA. 2004 Sep;10(9):1366-79
references.pmid: 15317974
references.citation: Biogerontology. 2009 Oct;10(5):627-36
references.pmid: 19123066
references.citation: Braz J Microbiol. 2008 Jul;39(3):561-8
references.pmid: 24031266
references.citation: Bioresour Technol. 2005 Jul;96(10):1175-82
references.pmid: 15683909