title: Development of chitosan-crosslinked nanofibrous PHBV guide for repair of nerve defects.
abstract: The aim of this study was to produce a chitosan-crosslinked nanofibrous biodegradable poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nerve conduit. The artificial scaffold was designed by electrospinning method, and cross-linked with chitosan by chemical method. The scaffolds were evaluated by microscopic, physical, and mechanical analyses, and cell culture assays with Schwann cells. Results of analyses showed a good resilience and compliance with movement as a neural graft. Cellular experiments showed a better cell adhesion and growth inside the crosslinked nanofibrous scaffolds compared with un-crosslinked ones. This neural conduit appears to have the right organization for testing in vivo nerve tissue engineering studies.
authors.affiliation: Department of Biomaterial Engineering, Tonekabon Branch, Islamic Azad University , Tonekabon , Iran.
authors.forename: Esmaeil
authors.initials: E
authors.lastname: Biazar
authors.forename: Saeed
authors.initials: S
authors.lastname: Heidari Keshel
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D048708:Cell Growth Processes; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry / Q000627:therapeutic use*; D048091:Guided Tissue Regeneration / Q000379:methods*; D057139:Nanofibers / Q000737:chemistry / Q000627:therapeutic use*; D009416:Nerve Regeneration; D011091:Polyesters / Q000737:chemistry; D051381:Rats; D012583:Schwann Cells / Q000502:physiology*; D009435:Synaptic Transmission; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000706:statistics & numerical data; D000094122:Polyhydroxybutyrates
chemical_list: D001672:Biocompatible Materials; D048271:Chitosan; D011091:Polyesters; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates
keywords: cell culture; chitosan-crosslinked nanofibrous PHBV; electrospinning; neural guide