title: Phosphorylation of the Drosophila transient receptor potential ion channel is regulated by the phototransduction cascade and involves several protein kinases and phosphatases.
abstract: Protein phosphorylation plays a cardinal role in regulating cellular processes in eukaryotes. Phosphorylation of proteins is controlled by protein kinases and phosphatases. We previously reported the light-dependent phosphorylation of the Drosophila transient receptor potential (TRP) ion channel at multiple sites. TRP generates the receptor potential upon stimulation of the photoreceptor cell by light. An eye-enriched protein kinase C (eye-PKC) has been implicated in the phosphorylation of TRP by in vitro studies. Other kinases and phosphatases of TRP are elusive. Using phosphospecific antibodies and mass spectrometry, we here show that phosphorylation of most TRP sites depends on the phototransduction cascade and the activity of the TRP ion channel. A candidate screen to identify kinases and phosphatases provided in vivo evidence for an involvement of eye-PKC as well as other kinases and phosphatases in TRP phosphorylation.
authors.affiliation: Department of Biosensorics, Institute of Physiology, Universität Hohenheim, Stuttgart, Germany.
authors.forename: Olaf
authors.initials: O
authors.lastname: Voolstra
authors.forename: Jonas-Peter
authors.initials: JP
authors.lastname: Bartels
authors.forename: Claudia
authors.initials: C
authors.lastname: Oberegelsbacher
authors.forename: Jens
authors.initials: J
authors.lastname: Pfannstiel
authors.forename: Armin
authors.initials: A
authors.lastname: Huber
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D015153:Blotting, Western; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D008027:Light; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009154:Mutation; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010766:Phosphorylation / Q000528:radiation effects; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D012694:Serine / Q000235:genetics / Q000378:metabolism; D013912:Threonine / Q000235:genetics / Q000378:metabolism; D050051:Transient Receptor Potential Channels / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014364:Tryptophan / Q000235:genetics / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D050051:Transient Receptor Potential Channels; C063169:trp protein, Drosophila; D013912:Threonine; D012694:Serine; D014364:Tryptophan; D011494:Protein Kinases; D011493:Protein Kinase C; D010744:Phosphoric Monoester Hydrolases
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