title: A high incidence of adjacent-1 meiotic segregation pattern, revealed by multicolor sperm FISH, in a carrier boar of a new reciprocal translocation t(6;16)(p13;q23).
abstract: Reciprocal translocations pose a serious problem in pig breeding due to the reduced fertility of the carriers. This paper presents a new reciprocal translocation in a phenotypically normal, but hypoprolific (20% reduction) boar. Chromosome banding as well as the FISH technique with the use of BAC and telomeric probes was applied for a detailed characterization of this chromosome rearrangement. The karyotype of the studied boar was described as 38,XY,t(6;16)(p13;q23). The meiotic segregation of the quadrivalent was studied in 1,071 sperms by multicolor FISH. The most frequent segregation patterns were alternate (47.5%) and adjacent 1 (41.9%), while adjacent 2 and 3:1 were less frequent at 1.2 and 9.2%, respectively. Surprisingly, the frequency of the adjacent-1 segregation appeared to be relatively high, when compared with human and pig reciprocal translocations studied by sperm FISH. Our study, along with a review of the literature, shows that a reduction of fertility in the carriers and the incidence of different segregation patterns of the quadrivalent may vary within a broad range, and both aspects seem to be unrelated. A need for obligatory karyotype screening programs of artificial insemination boars is emphasized.
authors.affiliation: Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Poznan, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Kociucka
authors.forename: I
authors.initials: I
authors.lastname: Szczerbal
authors.forename: S
authors.initials: S
authors.lastname: Bugaj
authors.forename: M
authors.initials: M
authors.lastname: Orsztynowicz
authors.forename: M
authors.initials: M
authors.lastname: Switonski
mesh_terms: D000015:Abnormalities, Multiple / Q000235:genetics / Q000662:veterinary; D000818:Animals; D001947:Breeding; D020090:Chromosome Segregation; D033481:Chromosomes, Mammalian / Q000235:genetics / Q000648:ultrastructure*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000662:veterinary*; D007248:Infertility, Male / Q000235:genetics / Q000662:veterinary*; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D013045:Species Specificity; D013094:Spermatozoa / Q000648:ultrastructure; D034421:Sus scrofa / Q000235:genetics*; D013552:Swine; D013553:Swine Diseases / Q000235:genetics*; D014178:Translocation, Genetic