title: Osteogenic potential of a chalcone in a critical-size defect in rat calvaria bone.
abstract: BACKGROUND
  This study describes the bone formation stimulated by the application of a type of chalcone to critical-size defects in rat calvarial bone.
  
  
  METHODS
  Sixty female Wistar rats were divided into 6 groups of 10 animals per group: control (no treatment), vehicle (vaseline) and the chalcone (1-phenyl-3-(4-chlorophenyl)-2-propen-1-one) suspended in vaseline at 10%. A critical-size defect of 5 mm was prepared using a trephine in the calvarial bone, after which the treatment was applied, in a single dose, according to the experimental group. The samples were evaluated macroscopically using ImageJ software, and histologically 30 and 45 days after surgery.
  
  
  RESULTS
  At 30 days after surgery, there was significant bone formation (p < 0.05) in the groups treated with chalcone, compared with the other groups. Many active osteoblasts were observed adjacent to the borders of the newly formed bone tissue. 45 days after surgery in the chalcone group, the surgical defects showed complete bone closure.
  
  
  CONCLUSIONS
  The results of this study suggest that chalcone has significant potential to induce the formation of new bone.
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Laboratory of Histology, University of Vale do Itajaí - UNIVALI, Itajaí, Santa Catarina, Brazil.
authors.forename: Xana Raquel
authors.initials: XR
authors.lastname: Ortolan
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Laboratory of Histology, University of Vale do Itajaí - UNIVALI, Itajaí, Santa Catarina, Brazil.
authors.forename: Bruna Proiss
authors.initials: BP
authors.lastname: Fenner
authors.affiliation: Department of Cell and Tissue Biology, Laboratory of Histology, University of Vale do Itajaí (UNIVALI), Itajaí, Santa Catarina, Brazil.
authors.forename: Telmo José
authors.initials: TJ
authors.lastname: Mezadri
authors.affiliation: Department of Sciences/Histology, Laboratory of Histology, University of Vale do Itajaí (UNIVALI), Itajaí, Santa Catarina, Brazil.
authors.forename: David Rivero
authors.initials: DR
authors.lastname: Tames
authors.affiliation: Department of Chemistry, Chemical-Pharmaceutical Investigations Center (NIQFAR)/CCS, University of Vale do Itajaí (UNIVALI), Itajaí, Santa Catarina, Brazil.
authors.forename: Rogério
authors.initials: R
authors.lastname: Corrêa
authors.affiliation: Department of Chemistry, Chemical-Pharmaceutical Investigations Center (NIQFAR)/CCS, University of Vale do Itajaí (UNIVALI), Itajaí, Santa Catarina, Brazil. Electronic address: fcamposbuzzi@univali.br.
authors.forename: Fátima
authors.initials: F
authors.lastname: de Campos Buzzi
mesh_terms: D000098:Acetophenones / Q000737:chemistry; D000818:Animals; D001547:Benzaldehydes / Q000737:chemistry; D001847:Bone Diseases / Q000188:drug therapy*; D001857:Bone Matrix / Q000187:drug effects / Q000473:pathology; D002113:Calcification, Physiologic / Q000187:drug effects; D002599:Chalcone / Q000138:chemical synthesis / Q000627:therapeutic use*; D047188:Chalcones / Q000627:therapeutic use*; D003238:Connective Tissue / Q000187:drug effects / Q000473:pathology; D005260:Female; D010006:Osteoblasts / Q000187:drug effects / Q000473:pathology; D010011:Osteocytes / Q000187:drug effects / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects*; D010577:Petrolatum; D014677:Pharmaceutical Vehicles; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000187:drug effects* / Q000473:pathology; D012972:Sodium Hydroxide / Q000737:chemistry; D013997:Time Factors
chemical_list: C000599124:1-phenyl-3-(4-chlorophenyl)-2-propen-1-one; D000098:Acetophenones; D001547:Benzaldehydes; D047188:Chalcones; D014677:Pharmaceutical Vehicles; D012972:Sodium Hydroxide; D002599:Chalcone; D010577:Petrolatum; C052044:4-chlorobenzaldehyde; C038699:acetophenone
keywords: Chalcone; Critical-size; Osteogenesis