title: Cohesin and the nucleolus constrain the mobility of spontaneous repair foci.
abstract: The regulation of chromatin mobility in response to DNA damage is important for homologous recombination in yeast. Anchorage reduces rates of recombination, whereas increased chromatin mobility correlates with more efficient homology search. Here we tracked the mobility and localization of spontaneous S-phase lesions bound by Rad52, and find that these foci have reduced movement, unlike enzymatically induced double-strand breaks. Moreover, spontaneous repair foci are positioned in the nuclear core, abutting the nucleolus. We show that cohesin and nucleolar integrity constrain the mobility of these foci, consistent with the notion that spontaneous, S-phase damage is preferentially repaired from the sister chromatid.
authors.affiliation: Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, Basel, Switzerland.
authors.forename: Vincent
authors.initials: V
authors.lastname: Dion
authors.forename: Véronique
authors.initials: V
authors.lastname: Kalck
authors.forename: Andrew
authors.initials: A
authors.lastname: Seeber
authors.forename: Thomas
authors.initials: T
authors.lastname: Schleker
authors.forename: Susan M
authors.initials: SM
authors.lastname: Gasser
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism*; D002466:Cell Nucleolus / Q000378:metabolism*; D002842:Chromatids / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004249:DNA Damage; D004260:DNA Repair; D059765:Homologous Recombination; D051721:Rad52 DNA Repair and Recombination Protein / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D000097722:Cohesins
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D051721:Rad52 DNA Repair and Recombination Protein; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D000097722:Cohesins; C497197:RAD52 protein, S cerevisiae
references.citation: Curr Biol. 2013 Jan 7;23(1):64-9
references.pmid: 23219725
references.citation: Curr Biol. 2002 Mar 19;12(6):439-45
references.pmid: 11909528
references.citation: Mol Cell. 2012 Oct 12;48(1):98-108
references.pmid: 22885006
references.citation: Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8276-82
references.pmid: 11459964
references.citation: Cell Mol Life Sci. 2012 May;69(9):1447-73
references.pmid: 22083606
references.citation: Curr Biol. 2004 Dec 14;14(23):2107-12
references.pmid: 15589152
references.citation: Genetics. 2009 Apr;181(4):1195-206
references.pmid: 19189942
references.citation: Genes Dev. 2012 Feb 15;26(4):369-83
references.pmid: 22345518
references.citation: Cell. 2013 Jan 31;152(3):417-29
references.pmid: 23374339
references.citation: Cell. 2004 Sep 17;118(6):699-713
references.pmid: 15369670
references.citation: PLoS Genet. 2013;9(1):e1003237
references.pmid: 23357952
references.citation: EMBO J. 2013 May 2;32(9):1307-21
references.pmid: 23563117
references.citation: Genes Dev. 2009 Apr 15;23(8):912-27
references.pmid: 19390086
references.citation: Mol Biol Cell. 2004 Apr;15(4):1736-45
references.pmid: 14742714
references.citation: Nat Cell Biol. 2007 Aug;9(8):923-31
references.pmid: 17643116
references.citation: Science. 2008 Oct 24;322(5901):597-602
references.pmid: 18948542
references.citation: Nucleic Acids Res. 2011 Aug;39(15):6489-99
references.pmid: 21511815
references.citation: Nat Methods. 2009 Oct;6(10):753-7
references.pmid: 19749762
references.citation: Annu Rev Genet. 2009;43:525-58
references.pmid: 19886810
references.citation: Curr Biol. 2004 Dec 14;14(23):2096-106
references.pmid: 15589151
references.citation: Mol Cell Biol. 2012 Jan;32(1):154-72
references.pmid: 22025679
references.citation: Nat Cell Biol. 2012 Apr 08;14(5):510-7
references.pmid: 22484485
references.citation: Cell. 2011 Mar 4;144(5):732-44
references.pmid: 21353298
references.citation: Mol Cell Biol. 2009 Feb;29(3):835-48
references.pmid: 19047366
references.citation: Nat Cell Biol. 2006 Sep;8(9):1032-4
references.pmid: 16892052
references.citation: Annu Rev Genet. 2010;44:113-39
references.pmid: 20690856
references.citation: Cell. 2013 Mar 14;152(6):1355-64
references.pmid: 23498942
references.citation: Mutat Res. 1996 Aug 17;355(1-2):71-89
references.pmid: 8781578
references.citation: Science. 2001 Dec 7;294(5549):2181-6
references.pmid: 11739961
references.citation: Mol Cell. 2009 Apr 10;34(1):26-35
references.pmid: 19362534
references.citation: Nat Cell Biol. 2013 Jun;15(6):694-9
references.pmid: 23644470
references.citation: EMBO Rep. 2006 Sep;7(9):919-26
references.pmid: 16888651
references.citation: Cell. 2000 Oct 27;103(3):375-86
references.pmid: 11081625
references.citation: Methods Enzymol. 2010;470:535-67
references.pmid: 20946824
references.citation: Nat Cell Biol. 2012 Apr 08;14(5):502-9
references.pmid: 22484486
references.citation: J Cell Biol. 2001 Jan 22;152(2):385-400
references.pmid: 11266454