title: A two-component system regulates hemin acquisition in Porphyromonas gingivalis.
abstract: Porphyromonas gingivalis is a Gram-negative oral anaerobe associated with infection of the periodontia. The organism has a small number of two-component signal transduction systems, and after comparing genome sequences of strains W83 and ATCC 33277 we discovered that the latter was mutant in histidine kinase (PGN_0752), while the cognate response regulator (PGN_0753) remained intact. Microarray-based transcriptional profiling and ChIP-seq assays were carried out with an ATCC 33277 transconjugant containing the functional histidine kinase from strain W83 (PG0719). The data showed that the regulon of this signal transduction system contained genes that were involved in hemin acquisition, including gingipains, at least three transport systems, as well as being self-regulated. Direct regulation by the response regulator was confirmed by electrophoretic mobility shift assays. In addition, the system appears to be activated by hemin and the regulator acts as both an activator and repressor.
authors.affiliation: Department of Microbiology, The Forsyth Institute, Cambridge, Massachusetts, United States of America.
authors.forename: Jodie C
authors.initials: JC
authors.lastname: Scott
authors.forename: Brian A
authors.initials: BA
authors.lastname: Klein
authors.forename: Ana
authors.initials: A
authors.lastname: Duran-Pinedo
authors.forename: Linden
authors.initials: L
authors.lastname: Hu
authors.forename: Margaret J
authors.initials: MJ
authors.lastname: Duncan
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016866:Bacteroidaceae Infections / Q000378:metabolism / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006427:Hemin / Q000378:metabolism*; D000071677:Histidine Kinase; D054884:Host-Pathogen Interactions; D006801:Humans; D007501:Iron / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D016966:Porphyromonas gingivalis / Q000235:genetics / Q000502:physiology*; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D001426:Bacterial Proteins; D006427:Hemin; D007501:Iron; D011494:Protein Kinases; D000071677:Histidine Kinase
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