title: In vitro antibacterial activities of ethanol extract of iranian propolis (EEIP) against fish pathogenic bacteria (Aeromonas hydrophila, Yersinia ruckeri & Streptococcus iniae).
abstract: The "in vitro" antibacterial activity of ethanol extract of propolis (EEIP) from Urmia, Iran was investigated against three prevalent species of fish bacterial pathogens including: Aeromonas hydrophila LMG 3770, Yersinia ruckeri LMG 3279 and Streptococcus iniae LMG 14520. In this study two standard susceptibility testing techniques (Micro-broth dilution method and Agar-well diffusion method) were used to evaluation of the antibacterial activity of EEIP against the mentioned micro-organisms. Also the chemical composition of propolis was determined by the method of Gas chromatography-mass spectrometry (GC-MS). Twenty-six compounds were identified by gas chromatography-mass spectrometry analysis. Results showed Chemical composition of EEIP contained significant amounts of flavonoids, Sesquiterpenes - mainly Eudesmol and Caryophyllene oxide - aromatic acid, and low amounts of aldehydes and triterpens. Furthermore the ethanol extract of propolis inhibited the growth of all examined micro-organisms with the highest antimicrobial activity against Gram-positive bacteria Streptococcus iniae. Ethanol did not influence the antimicrobial effect of EEIP. These antibacterial properties would warrant further studies on the clinical applications of propolis in aquaculture field.
authors.affiliation: Department of Pathobiology & Quality Control, Artemia &Aquatic Animal Research Institute, Urmia University , Urmia, West Azerbaijan , Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Tukmechi
authors.forename: Abdolghaffar
authors.initials: A
authors.lastname: Ownagh
authors.forename: Ali
authors.initials: A
authors.lastname: Mohebbat
keywords: antibacterial activity; bee glue; chemical composition; fish pathogenic bacteria; propolis
references.citation: Appl Environ Microbiol. 2002 Dec;68(12):6036-42
references.pmid: 12450826
references.citation: Arzneimittelforschung. 1993 May;43(5):607-9
references.pmid: 8329008
references.citation: Pharmazie. 1977 Nov;32(11):730
references.pmid: 609603
references.citation: J Nat Prod. 2005 Sep;68(9):1404-7
references.pmid: 16180824
references.citation: Arzneimittelforschung. 1977;27(4):889-90
references.pmid: 577469
references.citation: Braz J Microbiol. 2008 Oct;39(4):756-60
references.pmid: 24031303
references.citation: BMC Complement Altern Med. 2009 Jul 30;9:25
references.pmid: 19643008
references.citation: Acta Pharm. 2003 Dec;53(4):275-85
references.pmid: 14769234
references.citation: Int J Food Microbiol. 2005 Feb 1;98(2):131-4
references.pmid: 15747443
references.citation: Genet Mol Res. 2009;8(2):635-40
references.pmid: 19554760
references.citation: Food Chem Toxicol. 1998 Apr;36(4):347-63
references.pmid: 9651052
references.citation: Pharmazie. 1993 Oct;48(10):785-6
references.pmid: 8265716
references.citation: Phytother Res. 2001 Nov;15(7):561-71
references.pmid: 11746834
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007 Nov;104(5):709-16
references.pmid: 17964476
references.citation: Int J Food Microbiol. 2005 Jul 15;102(2):213-20
references.pmid: 15992620
references.citation: Antimicrob Agents Chemother. 2002 Oct;46(10):3133-41
references.pmid: 12234835
references.citation: J R Soc Med. 1990 Mar;83(3):159-60
references.pmid: 2182860
references.citation: Planta Med. 1994 Jun;60(3):222-7
references.pmid: 8073087
references.citation: Lett Appl Microbiol. 2000 Aug;31(2):174-7
references.pmid: 10972723