title: Population genetic analysis and origin discrimination of snow crab (Chionoecetes opilio) using microsatellite markers.
abstract: Major habitats for the snow crab Chionoecetes opilio are mostly found within the northwest Atlantic and North Pacific Oceans. However, the East Sea populations of C. opilio, along with its relative the red snow crab (C. japonicas), are two of the most important commercial crustacean species for fisheries on the east coast of the Korean Peninsula. The East Sea populations of C. opilio are facing declining resources due to overfishing and global climate change. Thus, an analysis of population structure is necessary for future management. Five Korean and one Russian group of C. opilio were analyzed using nine microsatellite markers that were recently developed using next-generation sequencing. No linkage disequilibrium was found between any pair of loci, indicating that the markers were independent. The number of alleles per locus varied from 4 to 18 with a mean of 12, and allelic richness per locus ranged from 4.0 to 17.1 across all populations with a mean of 9.7. The Hardy-Weinberg equilibrium test revealed significant deviation in three out of nine loci in some populations after sequential Bonferroni correction and all of them had higher expected heterozygosity than observed heterozygosity. Null alleles were presumed in four loci, which explained the homozygosity in three loci. The pairwise fixation index (F ST ) values among the five Korean snow crab populations did not differ significantly, but all of the pairwise F ST values between each of the Korean snow crab populations and the Russian snow crab population differed significantly. An UPGMA dendrogram revealed clear separation of the Russian snow crab population from the Korean snow crab populations. Assignment tests based on the allele distribution discriminated between Korean and Russian origins with 93 % accuracy. Therefore, the snow crab populations around the Korean Peninsula need to be managed separately from the populations in Bering Sea in global scale resource management. Also, this information can be used for identification of snow crab origin which is problematic in worldwide crab trade.
authors.forename: Jung-Ha
authors.initials: JH
authors.lastname: Kang
authors.forename: Jung-Youn
authors.initials: JY
authors.lastname: Park
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Kim
authors.forename: Hyun-Sook
authors.initials: HS
authors.lastname: Ko
mesh_terms: D000483:Alleles; D000818:Animals; D003386:Brachyura / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D056426:Genetic Loci / Q000235:genetics; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D006239:Haplotypes / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D010140:Pacific Ocean; D010802:Phylogeny; D056910:Republic of Korea; D012426:Russia; D012914:Snow
chemical_list: D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV
references.citation: Am J Bot. 2012 Feb;99(2):193-208
references.pmid: 22186186
references.citation: Trends Ecol Evol. 1996 Oct;11(10):424-9
references.pmid: 21237902
references.citation: Q Rev Biol. 1999 Mar;74(1):21-45
references.pmid: 10081813
references.citation: Mol Ecol. 1998 Mar;7(3):357-8
references.pmid: 9561792
references.citation: Integr Comp Biol. 2011 Oct;51(4):608-22
references.pmid: 21873643
references.citation: Genetics. 1978 Jul;89(3):583-90
references.pmid: 17248844
references.citation: Biotechniques. 1999 Sep;27(3):500-2, 504-7
references.pmid: 10489609
references.citation: Evol Bioinform Online. 2007 Feb 23;1:47-50
references.pmid: 19325852
references.citation: Mol Mar Biol Biotechnol. 1994 Oct;3(5):294-9
references.pmid: 7881515
references.citation: Evolution. 1984 Nov;38(6):1358-1370
references.pmid: 28563791
references.citation: J Hered. 2004 Nov-Dec;95(6):536-9
references.pmid: 15475402
references.citation: Biol Lett. 2005 Dec 22;1(4):450-3
references.pmid: 17148230