title: Occurrence of yeasts, pseudomonads and enteric bacteria in the oral cavity of patients undergoing head and neck radiotherapy.
abstract: The aim of this study was to evaluate the occurrence of yeasts, pseudomonads and enteric bacteria in the oral cavity of patients undergoing radiotherapy (RT) for treatment of head and neck cancer. Fifty patients receiving RT were examined before, during and 30 days after RT. Saliva, mucosa, and biofilm samples were collected and microorganisms were detected by culture and polymerase chain reaction (PCR). The most prevalent yeasts in patients submitted to RT were Candida albicans, C. tropicalis, C. krusei, C. glabrata and C. parapsilosis. Citrobacter, Enterobacter, Enterococcus, Klebsiella, Proteus, and Pseudomonas were the most frequently cultivated bacteria. Before RT, targeted bacteria were cultivated from 22.2% of edentulous patients and 16.6% of dentate patients; 30 days after RT, these microorganisms were recovered from 77.8% edentulous and 46.8% dentate patients. By PCR, these microorganisms were detected from all edentulous patients, 78.1% of dentate patients. The presence of Gram-negative enteric roads and fungi was particularly frequent in patients presenting mucositis level III or IV. Modifications in the oral environment due to RT treatment seem to facilitate the colonization of oral cavity by members of family Enterobacteriaceae, genera Enterococcus and Candida.
authors.affiliation: Departamento de Patologia e Propedêutica Clínica, Faculdade de Odontologia, Universidade Estadual Paulista , Araçatuba, SP , Brasil.
authors.forename: Elerson Júnior
authors.initials: EJ
authors.lastname: Gaetti-Jardim
authors.forename: Francisco Isaak Nicolas
authors.initials: FI
authors.lastname: Ciesielski
authors.forename: Fátima Regina Nunes
authors.initials: FR
authors.lastname: de Sousa
authors.forename: Francisca
authors.initials: F
authors.lastname: Nwaokorie
authors.forename: Christiane Marie
authors.initials: CM
authors.lastname: Schweitzer
authors.forename: Mario Júlio
authors.initials: MJ
authors.lastname: Avila-Campos
keywords: Cancer; Enterobacteriaceae; Prevalence; Radiotherapy
references.citation: Braz J Microbiol. 2008 Apr;39(2):257-61
references.pmid: 24031212
references.citation: Crit Rev Oral Biol Med. 2003;14(3):199-212
references.pmid: 12799323
references.citation: Braz Dent J. 2006;17(4):336-42
references.pmid: 17262149
references.citation: Braz J Microbiol. 2010 Oct;41(4):1056-64
references.pmid: 24031586
references.citation: Clin Radiol. 1988 May;39(3):291-4
references.pmid: 3293887
references.citation: J Clin Microbiol. 1999 Dec;37(12):3896-900
references.pmid: 10565903
references.citation: Microbiol Res. 2001;156(1):95-102
references.pmid: 11372661
references.citation: Braz J Microbiol. 2010 Apr;41(2):286-94
references.pmid: 24031493
references.citation: Oral Microbiol Immunol. 2001 Feb;16(1):1-9
references.pmid: 11169132
references.citation: Adv Med Sci. 2006;51 Suppl 1:86-90
references.pmid: 17458066
references.citation: Int J Radiat Oncol Biol Phys. 2000 Apr 1;47(1):1-12
references.pmid: 10758302
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1997 Aug;84(2):149-53
references.pmid: 9269016
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007 Jan;103(1):48-59
references.pmid: 17178494
references.citation: J Periodontol. 2004 Jan;75(1):57-65
references.pmid: 15025217
references.citation: Oral Surg Oral Med Oral Pathol. 1993 Aug;76(2):169-74
references.pmid: 8361726
references.citation: Oral Microbiol Immunol. 2002 Apr;17(2):79-84
references.pmid: 11929553
references.citation: Lett Appl Microbiol. 2007 May;44(5):488-94
references.pmid: 17451514
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007 Mar;103(3):355-8
references.pmid: 17321446
references.citation: J Am Dent Assoc. 2003 Jan;134(1):61-9; quiz 118-9
references.pmid: 12555958
references.citation: Oral Dis. 2008 Sep;14(6):541-9
references.pmid: 18208474
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007 Mar;103 Suppl:S66.e1-19
references.pmid: 17379158