title: Chaperones of the endoplasmic reticulum are required for Ve1-mediated resistance to Verticillium.
abstract: The tomato receptor-like protein (RLP) Ve1 mediates resistance to the vascular fungal pathogen Verticillium dahliae. To identify the proteins required for Ve1 function, we transiently expressed and immunopurified functional Ve1-enhanced green fluorescent protein (eGFP) from Nicotiana benthamiana leaves, followed by mass spectrometry. This resulted in the identification of peptides originating from the endoplasmic reticulum (ER)-resident chaperones HSP70 binding proteins (BiPs) and a lectin-type calreticulin (CRT). Knock-down of the different BiPs and CRTs in tomato resulted in compromised Ve1-mediated resistance to V. dahliae in most cases, showing that these chaperones play an important role in Ve1 functionality. Recently, it has been shown that one particular CRT is required for the biogenesis of the RLP-type Cladosporium fulvum resistance protein Cf-4 of tomato, as silencing of CRT3a resulted in a reduced pool of complex glycosylated Cf-4 protein. In contrast, knock-down of the various CRTs in N. benthamiana or N. tabacum did not result in reduced accumulation of mature complex glycosylated Ve1 protein. Together, this study shows that the BiP and CRT ER chaperones differentially contribute to Cf-4- and Ve1-mediated immunity.
authors.affiliation: Laboratory of Phytopathology, Wageningen University, 6708 PB, Wageningen, the Netherlands; Centre for BioSystems Genomics, 6700 AB, Wageningen, the Netherlands.
authors.forename: Thomas W H
authors.initials: TW
authors.lastname: Liebrand
authors.forename: Anja
authors.initials: A
authors.lastname: Kombrink
authors.forename: Zhao
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jan
authors.initials: J
authors.lastname: Sklenar
authors.forename: Alexandra M E
authors.initials: AM
authors.lastname: Jones
authors.forename: Silke
authors.initials: S
authors.lastname: Robatzek
authors.forename: Bart P H J
authors.initials: BP
authors.lastname: Thomma
authors.forename: Matthieu H A J
authors.initials: MH
authors.lastname: Joosten
mesh_terms: D037282:Calreticulin / Q000235:genetics; D060467:Disease Resistance; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D020868:Gene Silencing; D017343:Genes, Plant / Q000235:genetics; D006031:Glycosylation; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D018551:Solanum lycopersicum / Q000276:immunology* / Q000382:microbiology*; D018832:Molecular Chaperones / Q000378:metabolism*; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D057865:Plant Immunity; D010940:Plant Proteins / Q000378:metabolism*; D011485:Protein Binding; D014026:Nicotiana / Q000378:metabolism; D020172:Verticillium / Q000502:physiology*
chemical_list: D037282:Calreticulin; D018840:HSP70 Heat-Shock Proteins; D018832:Molecular Chaperones; D010940:Plant Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
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