title: Lack of association between GRIA1 polymorphisms and haplotypes with migraine without aura or response to triptans.
abstract: The present study was designed to replicate previous findings reporting a significant association between the rs548294 polymorphism at the glutamate receptor subunit GluR1 gene (GRIA1) and migraine without aura, either as a single marker or in haplotype combination with rs2195450. In addition, the role of GRIA1 polymorphisms and haplotypes was evaluated in migraine patients without aura as predictive factors for consistency in headache response to triptans. Analysis of rs548294 and rs2195450 polymorphisms of GRIA1 was conducted by Real-time PCR allelic discrimination assay in 186 migraine patients without aura and 312 healthy controls, respectively. In the logistic regression analysis adjusted for gender and age, genotype and haplotype frequencies for the two polymorphisms did not significantly differ between migraine patients without aura and controls. In addition, no evidence of association was found between GRIA1 polymorphisms/haplotypes and consistent response to triptans. This study failed to replicate previously reported association between GRIA1 rs548294 and migraine without aura, either as single marker or when analyzed in haplotype combination with rs2195450. In addition, no evidence was found for a relevant role of GRIA1 polymorphisms and haplotypes as modulating factors of headache response to triptans.
authors.affiliation: Department of Pharmaceutical Sciences, Università del Piemonte Orientale "A. Avogadro", Largo Donegani 2, 28100, Novara, Italy.
authors.forename: Sarah
authors.initials: S
authors.lastname: Cargnin
authors.forename: Michele
authors.initials: M
authors.lastname: Viana
authors.forename: Daniela
authors.initials: D
authors.lastname: Mittino
authors.forename: Giorgio
authors.initials: G
authors.lastname: Bellomo
authors.forename: Cristina
authors.initials: C
authors.lastname: Tassorelli
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Nappi
authors.forename: Pier Luigi
authors.initials: PL
authors.lastname: Canonico
authors.forename: Salvatore
authors.initials: S
authors.lastname: Terrazzino
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D020326:Migraine without Aura / Q000188:drug therapy* / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018091:Receptors, AMPA / Q000235:genetics*; D014363:Tryptamines / Q000627:therapeutic use*
chemical_list: D018091:Receptors, AMPA; D014363:Tryptamines
references.citation: Headache. 2003 Mar;43(3):235-44
references.pmid: 12603642
references.citation: J Cereb Blood Flow Metab. 1992 Mar;12(2):223-9
references.pmid: 1312539
references.citation: Nat Genet. 2010 Oct;42(10):869-73
references.pmid: 20802479
references.citation: Histochem J. 1991 Apr;23(4):171-9
references.pmid: 1684179
references.citation: Brain Res. 2001 Dec 27;923(1-2):45-9
references.pmid: 11743971
references.citation: J Headache Pain. 2012 Jan;13(1):1-9
references.pmid: 22072275
references.citation: Mol Pain. 2008 Apr 16;4:12
references.pmid: 18416824
references.citation: Eur J Neurosci. 2006 Sep;24(6):1517-34
references.pmid: 17004916
references.citation: Headache. 2013 Feb;53(2):401-12
references.pmid: 23278104
references.citation: Bioinformatics. 2006 Aug 1;22(15):1928-9
references.pmid: 16720584
references.citation: Nat Genet. 2011 Jun 12;43(7):695-8
references.pmid: 21666692
references.citation: Neuroreport. 2001 Jun 13;12(8):1589-91
references.pmid: 11409721
references.citation: Control Clin Trials. 1986 Sep;7(3):177-88
references.pmid: 3802833
references.citation: Ann Intern Med. 1997 Nov 1;127(9):820-6
references.pmid: 9382404
references.citation: Twin Res Hum Genet. 2006 Feb;9(1):54-63
references.pmid: 16611468
references.citation: Nat Genet. 2012 Jun 10;44(7):777-82
references.pmid: 22683712
references.citation: Headache. 2007 Jun;47(6):842-7
references.pmid: 17578532
references.citation: Eur J Pharmacol. 2010 Sep 1;641(2-3):82-7
references.pmid: 20488209
references.citation: Philos Trans R Soc Lond B Biol Sci. 2002 Dec 29;357(1428):1759-66
references.pmid: 12626010
references.citation: Eur J Hum Genet. 2011 Aug;19(8):901-7
references.pmid: 21448238
references.citation: Headache. 2013 Sep;53(8):1245-9
references.pmid: 23772601
references.citation: CNS Neurol Disord Drug Targets. 2007 Aug;6(4):251-7
references.pmid: 17691981
references.citation: Genet Epidemiol. 2004 Apr;26(3):231-44
references.pmid: 15022209
references.citation: CNS Spectr. 2003 Jun;8(6):446-9
references.pmid: 12858134
references.citation: Twin Res. 2003 Oct;6(5):422-31
references.pmid: 14624726
references.citation: BMC Med Genet. 2010 Jun 25;11:103
references.pmid: 20579352
references.citation: Cephalalgia. 1997 Jun;17(4):488-91; discussion 487
references.pmid: 9209767