title: Efficient disruption of Zebrafish genes using a Gal4-containing gene trap.
abstract: BACKGROUND
  External development and optical transparency of embryos make zebrafish exceptionally suitable for in vivo insertional mutagenesis using fluorescent proteins to visualize expression patterns of mutated genes. Recently developed Gene Breaking Transposon (GBT) vectors greatly improve the fidelity and mutagenicity of transposon-based gene trap vectors.
  
  
  RESULTS
  We constructed and tested a bipartite GBT vector with Gal4-VP16 as the primary gene trap reporter. Our vector also contains a UAS:eGFP cassette for direct detection of gene trap events by fluorescence. To confirm gene trap events, we generated a UAS:mRFP tester line. We screened 270 potential founders and established 41 gene trap lines. Three of our gene trap alleles display homozygous lethal phenotypes ranging from embryonic to late larval: nsf( tpl6), atp1a3a(tpl10) and flr(tpl19). Our gene trap cassette is flanked by direct loxP sites, which enabled us to successfully revert nsf( tpl6), atp1a3a(tpl10) and flr(tpl19) gene trap alleles by injection of Cre mRNA. The UAS:eGFP cassette is flanked by direct FRT sites. It can be readily removed by injection of Flp mRNA for use of our gene trap alleles with other tissue-specific GFP-marked lines. The Gal4-VP16 component of our vector provides two important advantages over other GBT vectors. The first is increased sensitivity, which enabled us to detect previously unnoticed expression of nsf in the pancreas. The second advantage is that all our gene trap lines, including integrations into non-essential genes, can be used as highly specific Gal4 drivers for expression of other transgenes under the control of Gal4 UAS.
  
  
  CONCLUSIONS
  The Gal4-containing bipartite Gene Breaking Transposon vector presented here retains high specificity for integrations into genes, high mutagenicity and revertibility by Cre. These features, together with utility as highly specific Gal4 drivers, make gene trap mutants presented here especially useful to the research community.
authors.affiliation: Department of Biology, College of Science and Technology, Temple University, Philadelphia, PA 19122, USA. darius@temple.edu.
authors.forename: Jorune
authors.initials: J
authors.lastname: Balciuniene
authors.forename: Danielle
authors.initials: D
authors.lastname: Nagelberg
authors.forename: Kathleen T
authors.initials: KT
authors.lastname: Walsh
authors.forename: Diana
authors.initials: D
authors.lastname: Camerota
authors.forename: Daphné
authors.initials: D
authors.lastname: Georlette
authors.forename: Frédéric
authors.initials: F
authors.lastname: Biemar
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Bellipanni
authors.forename: Darius
authors.initials: D
authors.lastname: Balciunas
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics; D004251:DNA Transposable Elements; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D016254:Mutagenesis, Insertional / Q000379:methods*; D015027:Zebrafish / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D029961:Zebrafish Proteins; D049452:Green Fluorescent Proteins
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