title: Spectroscopic and electrochemical characterization of the [NiFeSe] hydrogenase from Desulfovibrio vulgaris Miyazaki F: reversible redox behavior and interactions between electron transfer centers.
abstract: Characterizing a new hydrogenase: The newly isolated [NiFeSe] hydrogenase from Desulfovibrio vulgaris Miyazaki F displays catalytic properties distinct from other hydrogenase proteins. Here we apply site-specific spectroscopic and electrochemical techniques to characterize these unique features at the molecular level.
authors.affiliation: Max Planck Institute for Chemical Energy Conversion, Stiftstrasse 34-36, Muelheim an der Ruhr 45470 (Germany).
authors.forename: Jana
authors.initials: J
authors.lastname: Riethausen
authors.forename: Olaf
authors.initials: O
authors.lastname: Rüdiger
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Gärtner
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Lubitz
authors.forename: Hannah S
authors.initials: HS
authors.lastname: Shafaat
mesh_terms: D016969:Desulfovibrio vulgaris / Q000201:enzymology*; D055664:Electrochemical Techniques; D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport; D004583:Electrons; D006864:Hydrogenase / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: C058560:nickel-iron-selenium hydrogenase; D006864:Hydrogenase
keywords: electrochemistry; hydrogenase; iron-sulfur clusters; selenium; selenocysteine; spectroscopy