title: Characterization of a novel polyclonal anti-hypusine antibody.
abstract: The translation factor eIF5A is the only protein known to contain the amino acid hypusine, which is formed posttranslationally. Hypusinated eIF5A is necessary for cellular proliferation and responses to extracellular stressors, and has been proposed as a target for pharmacologic therapy. Here, we provide the first comprehensive characterization of a novel polyclonal antibody (IU-88) that specifically recognizes the hypusinated eIF5A. IU-88 will be useful for the investigation of eIF5A biology and for the development of assays recognizing hypusinated eIF5A.
authors.affiliation: Departments of Medicine, Cellular and Integrative Physiology, and Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202 USA.
authors.forename: Yurika
authors.initials: Y
authors.lastname: Nishiki
authors.forename: Thomas B
authors.initials: TB
authors.lastname: Farb
authors.forename: Jessica
authors.initials: J
authors.lastname: Friedrich
authors.forename: Krister
authors.initials: K
authors.lastname: Bokvist
authors.forename: Raghavendra G
authors.initials: RG
authors.lastname: Mirmira
authors.forename: Bernhard
authors.initials: B
authors.lastname: Maier
keywords: Antibody; Cell lines; Hypusine; Recombinant proteins; eIF5A
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