title: Insight into the structural stability of wild type and mutants of the tobacco etch virus protease with molecular dynamics simulations.
abstract: The efficiency and high specificity of tobacco etch virus protease (TEVp) has made it widely used for cleavage of recombinant fusion proteins. However, TEVp suffers from a few intrinsic defects such as self-cleavage, poorly expressed in E. coli and less soluble. So some mutants were designed to improve it, such as S219V, T17S/N68D/I77V and L56V/S135G etc. MD simulations for the WT TEVp and its mutants were performed to explore the underlying dynamic effects of mutations on TEVp. Although the globular domains are fairly conserved, the three mutations have diverse effects on the dynamics properties of TEVp, including the elongation of β-sheet, conversion of loop to helix and the flexibility of active core. Our present study indicates that the three mutants for TEVp can change their secondary structure and tend to form more helixes and sheets to improve stability. The study also helps us to understand the effects of some mutations on TEVp, provides us insights into the change of them at the atomic level and gives a potential rational method to design an improved protein.
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Guo-Fei
authors.initials: GF
authors.lastname: Zhu
authors.forename: Si-Yan
authors.initials: SY
authors.lastname: Ren
authors.forename: Yong-Guang
authors.initials: YG
authors.lastname: Han
authors.forename: Yue
authors.initials: Y
authors.lastname: Luo
authors.forename: Lin-Fang
authors.initials: LF
authors.lastname: Du
mesh_terms: D010450:Endopeptidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D009154:Mutation; D011993:Recombinant Fusion Proteins / Q000235:genetics
chemical_list: D011993:Recombinant Fusion Proteins; D010450:Endopeptidases; C099488:TEV protease
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