title: FishMicrosat: a microsatellite database of commercially important fishes and shellfishes of the Indian subcontinent.
abstract: BACKGROUND
  Microsatellite DNA is one of many powerful genetic markers used for the construction of genetic linkage maps and the study of population genetics. The biological databases in public domain hold vast numbers of microsatellite sequences for many organisms including fishes. The microsatellite data available in these data sources were extracted and managed into a database that facilitates sequences analysis and browsing relevant information. The system also helps to design primer sequences for flanking regions of repeat loci for PCR identification of polymorphism within populations.
  
  
  METHODS
  FishMicrosat is a database of microsatellite sequences of fishes and shellfishes that includes important aquaculture species such as Lates calcarifer, Ctenopharyngodon idella, Hypophthalmichthys molitrix, Penaeus monodon, Labeo rohita, Oreochromis niloticus, Fenneropenaeus indicus and Macrobrachium rosenbergii. The database contains 4398 microsatellite sequences of 41 species belonging to 15 families from the Indian subcontinent. GenBank of NCBI was used as a prime data source for developing the database. The database presents information about simple and compound microsatellites, their clusters and locus orientation within sequences. The database has been integrated with different tools in a web interface such as primer designing, locus finding, mapping repeats, detecting similarities among sequences across species, and searching using motifs and keywords. In addition, the database has the ability to browse information on the top 10 families and the top 10 species, through record overview.
  
  
  CONCLUSIONS
  FishMicrosat database is a useful resource for fish and shellfish microsatellite analyses and locus identification across species, which has important applications in population genetics, evolutionary studies and genetic relatedness among species. The database can be expanded further to include the microsatellite data of fishes and shellfishes from other regions and available information on genome sequencing project of species of aquaculture importance.
authors.affiliation: Division of Molecular Biology and Biotechnology, National Bureau of Fish Genetic Resources, Lucknow 226002, India. mahender@scientist.com.
authors.forename: Naresh Sahebrao
authors.initials: NS
authors.lastname: Nagpure
authors.forename: Iliyas
authors.initials: I
authors.lastname: Rashid
authors.forename: Rameshwar
authors.initials: R
authors.lastname: Pati
authors.forename: Ajey Kumar
authors.initials: AK
authors.lastname: Pathak
authors.forename: Mahender
authors.initials: M
authors.lastname: Singh
authors.forename: Shri Prakash
authors.initials: SP
authors.lastname: Singh
authors.forename: Uttam Kumar
authors.initials: UK
authors.lastname: Sarkar
mesh_terms: D000465:Algorithms; D000818:Animals; D030561:Databases, Nucleic Acid; D005399:Fishes / Q000235:genetics*; D007194:India; D020407:Internet; D018895:Microsatellite Repeats; D033561:Penaeidae / Q000235:genetics*; D012758:Shellfish; D014584:User-Computer Interface
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