title: Renal capsule xenografting and subcutaneous pellet implantation for the evaluation of prostate carcinogenesis and benign prostatic hyperplasia.
abstract: New therapies for two common prostate diseases, prostate cancer (PrCa) and benign prostatic hyperplasia (BPH), depend critically on experiments evaluating their hormonal regulation. Sex steroid hormones (notably androgens and estrogens) are important in PrCa and BPH; we probe their respective roles in inducing prostate growth and carcinogenesis in mice with experiments using compressed hormone pellets. Hormone and/or drug pellets are easily manufactured with a pellet press, and surgically implanted into the subcutaneous tissue of the male mouse host. We also describe a protocol for the evaluation of hormonal carcinogenesis by combining subcutaneous hormone pellet implantation with xenografting of prostate cell recombinants under the renal capsule of immunocompromised mice. Moreover, subcutaneous hormone pellet implantation, in combination with renal capsule xenografting of BPH tissue, is useful to better understand hormonal regulation of benign prostate growth, and to test new therapies targeting sex steroid hormone pathways.
authors.affiliation: Department of Urology, University of Wisconsin-Madison.
authors.forename: Tristan M
authors.initials: TM
authors.lastname: Nicholson
authors.forename: Kristen S
authors.initials: KS
authors.lastname: Uchtmann
authors.forename: Conrad D
authors.initials: CD
authors.lastname: Valdez
authors.forename: Ashleigh B
authors.initials: AB
authors.lastname: Theberge
authors.forename: Tihomir
authors.initials: T
authors.lastname: Miralem
authors.forename: William A
authors.initials: WA
authors.lastname: Ricke
mesh_terms: D000818:Animals; D063646:Carcinogenesis; D006728:Hormones / Q000008:administration & dosage*; D006801:Humans; D007668:Kidney / Q000601:surgery; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation / Q000379:methods*; D011470:Prostatic Hyperplasia / Q000139:chemically induced / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000139:chemically induced / Q000473:pathology* / Q000601:surgery; D014183:Transplantation, Heterologous / Q000379:methods*
chemical_list: D006728:Hormones
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