title: Advancing allele group-specific amplification of the complete HLA-C gene--isolation of novel alleles from three allele groups (C*04, C*07 and C*08).
abstract: A variety of strategies have been designed for sequence-based HLA typing (SBT) and for the isolation of new human leucocyte antigen (HLA) alleles, but unambiguous characterization of complete genomic sequences remains a challenge. We recently reported a simple method for the group-specific amplification (GSA) and sequencing of a full-length C*04 genomic sequence in isolation from the accompanying allele. Here we build on this strategy and present homologous methods that enable the isolation of HLA-C alleles belonging to another two allele groups. Using this approach, which can be applied to sequence-based typing in some clinical settings, we have successfully characterized three novel HLA-C alleles (C*04:128, C*07:01:01:02, and C*08:62).
authors.affiliation: Laboratorio de Inmunogenética-HLA, Hospital Universitario Puerta de Hierro, Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Cisneros
authors.forename: N
authors.initials: N
authors.lastname: Martínez-Pomar
authors.forename: M
authors.initials: M
authors.lastname: Vilches
authors.forename: P
authors.initials: P
authors.lastname: Martín
authors.forename: R
authors.initials: R
authors.lastname: de Pablo
authors.forename: N
authors.initials: N
authors.lastname: Nuñez Del Prado
authors.forename: A
authors.initials: A
authors.lastname: Nieto
authors.forename: N
authors.initials: N
authors.lastname: Matamoros
authors.forename: M
authors.initials: M
authors.lastname: Moraru
authors.forename: C
authors.initials: C
authors.lastname: Vilches
mesh_terms: D020121:5' Untranslated Regions; D000483:Alleles; D001483:Base Sequence; D005091:Exons; D015236:HLA-C Antigens / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006650:Histocompatibility Testing; D006801:Humans; D007438:Introns; D008958:Models, Molecular; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D020121:5' Untranslated Regions; D015236:HLA-C Antigens
keywords: genomic DNA; human leucocyte antigen-C; human variation; new allele; sequence-based typing; untranslated regions