title: Preparation and in vitro anti-tumor properties of toad venom extract-loaded solid lipid nanoparticles.
abstract: In this study, we prepared solid lipid nanoparticles (TV-SLNs) loaded with toad venom extract and investigated their anti-tumor effects in vitro in HeLa and SKOV-3 cells. TV-SLNs were prepared using a cold homogenization technique, and the formulation was optimized by central composite design and response surface methods. The anti-tumor activities of TV-SLNs were evaluated by analyzing cell division and cell cycle distribution by using the MTT assay and flow cytometry. After incubation with TV-SLNs, the growth of both HeLa and SKOV-3 cells was inhibited significantly. The percentage of HeLa cells in G0/G1 phase decreased, whereas that in the S and G2/M phases increased. Thus, the S and G2/M phases were blocked after the incubation of HeLa cells with TV-SLNs for 24 h. In contrast, the percentage of SKOV-3 cells in G0/G1 phase increased and then decreased in S and G2/M phases, with the G0/G1 phase being blocked after incubation with TV-SLNs for 24 h. Our results demonstrate that TV-SLNs inhibited the fissiparism of HeLa and SKOV-3 cells in a time-and dose-dependent manner. TV-SLNs may be effective as a novel TV vaginal delivery system for the treatment of cervical and ovarian cancers.
authors.affiliation: School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Su-Juan
authors.initials: SJ
authors.lastname: Zhang
authors.forename: Yong-Tai
authors.initials: YT
authors.lastname: Zhang
authors.forename: Ji-Hui
authors.initials: JH
authors.lastname: Zhao
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Shen
authors.forename: Feng
authors.initials: F
authors.lastname: Shi
authors.forename: Nian-Ping
authors.initials: NP
authors.lastname: Feng
mesh_terms: D000282:Administration, Intravaginal; D000664:Amphibian Venoms / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D004339:Drug Compounding; D016503:Drug Delivery Systems; D015195:Drug Design; D005260:Female; D006367:HeLa Cells; D006801:Humans; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D010051:Ovarian Neoplasms / Q000188:drug therapy; D010316:Particle Size; D013535:Suspensions; D013778:Tetrazolium Salts; D013844:Thiazoles; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy
chemical_list: D000664:Amphibian Venoms; D000970:Antineoplastic Agents; D013535:Suspensions; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue