title: Specific features of telomerase RNA from Hansenula polymorpha.
abstract: Telomerase, a ribonucleoprotein, is responsible for the maintenance of eukaryotic genome integrity by replicating the ends of chromosomes. The core enzyme comprises the conserved protein TERT and an RNA subunit (TER) that, in contrast, displays large variations in size and structure. Here, we report the identification of the telomerase RNA from thermotolerant yeast Hansenula polymorpha (HpTER) and describe its structural features. We show further that the H. polymorpha telomerase reverse transcribes the template beyond the predicted boundary and adds a nontelomeric dT in vitro. Sequencing of the chromosomal ends revealed that this nucleotide is specifically present as a terminal nucleotide at the 3' end of telomeres. Mutational analysis of HpTER confirmed that the incorporation of dT functions to limit telomere length in this species.
authors.forename: Elena M
authors.initials: EM
authors.lastname: Smekalova
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Malyavko
authors.forename: Maria I
authors.initials: MI
authors.lastname: Zvereva
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Mardanov
authors.forename: Nikolai V
authors.initials: NV
authors.lastname: Ravin
authors.forename: Konstantin G
authors.initials: KG
authors.lastname: Skryabin
authors.forename: Eric
authors.initials: E
authors.lastname: Westhof
authors.forename: Olga A
authors.initials: OA
authors.lastname: Dontsova
mesh_terms: D001483:Base Sequence; D055786:Gene Knockout Techniques; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010843:Pichia / Q000235:genetics*; D012313:RNA / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017423:Sequence Analysis, RNA; D019098:Telomerase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D059505:Telomere Homeostasis; D013941:Thymine
chemical_list: D012331:RNA, Fungal; C422952:telomerase RNA; D012313:RNA; D019098:Telomerase; D013941:Thymine
keywords: telomerase RNA; telomere biogenesis
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