title: Annotation of differentially expressed genes in the somatic embryogenesis of musa and their location in the banana genome.
abstract: Analysis of cDNA-AFLP was used to study the genes expressed in zygotic and somatic embryogenesis of Musa acuminata Colla ssp. malaccensis, and a comparison was made between their differential transcribed fragments (TDFs) and the sequenced genome of the double haploid- (DH-) Pahang of the malaccensis subspecies that is available in the network. A total of 253 transcript-derived fragments (TDFs) were detected with apparent size of 100-4000 bp using 5 pairs of AFLP primers, of which 21 were differentially expressed during the different stages of banana embryogenesis; 15 of the sequences have matched DH-Pahang chromosomes, with 7 of them being homologous to gene sequences encoding either known or putative protein domains of higher plants. Four TDF sequences were located in all Musa chromosomes, while the rest were located in one or two chromosomes. Their putative individual function is briefly reviewed based on published information, and the potential roles of these genes in embryo development are discussed. Thus the availability of the genome of Musa and the information of TDFs sequences presented here opens new possibilities for an in-depth study of the molecular and biochemical research of zygotic and somatic embryogenesis of Musa.
authors.affiliation: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C., C 43 No. 130, Col. Chuburná de Hidalgo, Mérida, Yucatán, C.P. 97200, Mexico.
authors.forename: Josefina Ines
authors.initials: JI
authors.lastname: Maldonado-Borges
authors.forename: José Roberto
authors.initials: JR
authors.lastname: Ku-Cauich
authors.forename: Rosa Maria
authors.initials: RM
authors.lastname: Escobedo-Graciamedrano
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D018507:Gene Expression Regulation, Developmental; D018745:Genome, Plant; D058977:Molecular Sequence Annotation; D028521:Musa / Q000235:genetics*; D058439:Plant Somatic Embryogenesis Techniques; D012639:Seeds / Q000196:embryology / Q000235:genetics
references.citation: Front Biosci. 2004 May 01;9:1611-25
references.pmid: 14977572
references.citation: BMC Plant Biol. 2011 May 21;11:95
references.pmid: 21599988
references.citation: Brief Funct Genomic Proteomic. 2005 Feb;3(4):362-71
references.pmid: 15814026
references.citation: Plant J. 1996 May;9(5):745-53
references.pmid: 8653120
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:97-131
references.pmid: 15012205
references.citation: Anal Biochem. 1991 Jul;196(1):80-3
references.pmid: 1716076
references.citation: Plant Physiol. 2009 Dec;151(4):1889-901
references.pmid: 19776160
references.citation: Plant Signal Behav. 2011 Dec;6(12):1993-6
references.pmid: 22105026
references.citation: Mol Biotechnol. 2011 Mar;47(3):189-99
references.pmid: 20803102
references.citation: Trends Biochem Sci. 2002 Apr;27(4):168-70
references.pmid: 11943536
references.citation: Nucleic Acids Res. 1995 Nov 11;23(21):4407-14
references.pmid: 7501463
references.citation: Funct Integr Genomics. 2006 Jan;6(1):36-46
references.pmid: 15856348
references.citation: Nature. 2012 Aug 9;488(7410):213-7
references.pmid: 22801500
references.citation: Plant Physiol. 2009 Mar;149(3):1289-301
references.pmid: 19168645
references.citation: Biotechnol Adv. 2012 Nov-Dec;30(6):1697-706
references.pmid: 22960619