title: Identification of CtpL as a chromosomally encoded chemoreceptor for 4-chloroaniline and catechol in Pseudomonas aeruginosa PAO1.
abstract: Bacterial chemotaxis influences the ability of bacteria to survive and thrive in most environments, including polluted ones. Despite numerous reports of the phenotypic characterization of chemotactic bacteria, only a few molecular details of chemoreceptors for aromatic pollutants have been described. In this study, the molecular basis of chemotaxis toward an environmentally toxic chlorinated aromatic pollutant, 4-chloroaniline (4CA), was evaluated. Among the three Pseudomonas spp. tested, Pseudomonas aeruginosa PAO1 exhibited positive chemotaxis both to the nonmetabolizable 4CA, where 4-chloroacetanilide was formed as a dead-end transformation product, and to the metabolizable catechol. Molecular analysis of all 26 mutants with a disrupted methyl-accepting chemotaxis gene revealed that CtpL, a chromosomally encoded chemoreceptor, was responsible for the positive chemotactic response toward 4CA. Since CtpL has previously been described to be a major chemoreceptor for inorganic phosphate at low concentrations in PAO1, this report describes a fortuitous ability of CtpL to function toward aromatic pollutants. In addition, its regulation not only was dependent on the presence of the chemoattractant inducer but also was regulated by conditions of phosphate starvation. These results expand the range of known chemotactic transducers and their function in the environmental bacterium PAO1.
authors.affiliation: Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Alisa S
authors.initials: AS
authors.lastname: Vangnai
authors.forename: Kazuki
authors.initials: K
authors.lastname: Takeuchi
authors.forename: Shota
authors.initials: S
authors.lastname: Oku
authors.forename: Naoya
authors.initials: N
authors.lastname: Kataoka
authors.forename: Tisana
authors.initials: T
authors.lastname: Nitisakulkan
authors.forename: Takahisa
authors.initials: T
authors.lastname: Tajima
authors.forename: Junichi
authors.initials: J
authors.lastname: Kato
mesh_terms: D000814:Aniline Compounds / Q000378:metabolism*; D002396:Catechols / Q000378:metabolism*; D002633:Chemotaxis; D004785:Environmental Pollutants / Q000378:metabolism*; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*
chemical_list: D000814:Aniline Compounds; D002396:Catechols; D004785:Environmental Pollutants; D011956:Receptors, Cell Surface; C034221:catechol; C004658:4-chloroaniline
references.citation: J Bacteriol. 1984 Nov;160(2):622-8
references.pmid: 6501217
references.citation: Appl Environ Microbiol. 2000 Sep;66(9):4098-104
references.pmid: 10966434
references.citation: Appl Environ Microbiol. 2007 Dec;73(23):7793-5
references.pmid: 17933940
references.citation: Chemosphere. 2010 Sep;81(2):177-86
references.pmid: 20637490
references.citation: J Bacteriol. 1981 Jan;145(1):145-55
references.pmid: 6780510
references.citation: Microbiology (Reading). 1997 Oct;143 ( Pt 10):3223-3229
references.pmid: 9353923
references.citation: Appl Environ Microbiol. 1988 Jun;54(6):1399-404
references.pmid: 3415220
references.citation: Biosci Biotechnol Biochem. 1999 Jan;63(1):155-61
references.pmid: 10052136
references.citation: J Biosci Bioeng. 2008 Jul;106(1):1-7
references.pmid: 18691523
references.citation: BMC Microbiol. 2012 Feb 01;12:19
references.pmid: 22292983
references.citation: Microbiology (Reading). 2000 Oct;146 ( Pt 10):2345-2350
references.pmid: 11021911
references.citation: FEMS Microbiol Lett. 2004 Feb 16;231(2):247-52
references.pmid: 14987771
references.citation: J Bacteriol. 2001 Aug;183(16):4681-6
references.pmid: 11466269
references.citation: Appl Environ Microbiol. 1992 Jul;58(7):2250-4
references.pmid: 16348736
references.citation: Environ Microbiol. 2011 Jul;13(7):1733-44
references.pmid: 21605304
references.citation: J Hazard Mater. 2011 Feb 28;186(2-3):1300-7
references.pmid: 21177022
references.citation: J Biosci Bioeng. 2005 Apr;99(4):396-402
references.pmid: 16233808
references.citation: J Bacteriol. 1999 May;181(10):3310-6
references.pmid: 10322041
references.citation: J Bacteriol. 2007 May;189(9):3502-14
references.pmid: 17277060
references.citation: J Biotechnol. 2012 Jul 31;160(1-2):25-32
references.pmid: 22321573
references.citation: J Bacteriol. 2000 Jun;182(12):3400-4
references.pmid: 10852870
references.citation: J Appl Microbiol. 2013 Apr;114(4):923-33
references.pmid: 23163356
references.citation: Microbiology (Reading). 2000 Oct;146 ( Pt 10):2351-2364
references.pmid: 11021912
references.citation: Appl Environ Microbiol. 1997 Oct;63(10):4111-5
references.pmid: 9327579
references.citation: Appl Environ Microbiol. 2012 Sep;78(17):6365-8
references.pmid: 22729534
references.citation: Mikrobiologiia. 2002 Sep-Oct;71(5):581-95
references.pmid: 12449623
references.citation: Appl Environ Microbiol. 2002 Feb;68(2):968-72
references.pmid: 11823246
references.citation: J Bacteriol. 1992 Aug;174(15):5149-51
references.pmid: 1629173
references.citation: J Biosci Bioeng. 2004;97(3):184-90
references.pmid: 16233612
references.citation: Appl Environ Microbiol. 2009 Sep;75(17):5481-8
references.pmid: 19581468
references.citation: Nature. 2000 Aug 31;406(6799):959-64
references.pmid: 10984043
references.citation: Appl Environ Microbiol. 2004 Jan;70(1):285-92
references.pmid: 14711654
references.citation: Adv Appl Microbiol. 2009;66:53-75
references.pmid: 19203648
references.citation: Appl Environ Microbiol. 1988 Oct;54(10):2432-8
references.pmid: 3144244
references.citation: FEMS Microbiol Ecol. 2007 May;60(2):322-8
references.pmid: 17374130
references.citation: FEMS Microbiol Rev. 2009 Mar;33(2):324-75
references.pmid: 19178567
references.citation: J Bacteriol. 1995 Dec;177(24):7019-25
references.pmid: 8522505
references.citation: Gene. 1991 Jan 2;97(1):109-21
references.pmid: 1899844