title: Isolation and fusion of protoplasts from the phytopathogenic fungus sclerotium rolfsii(sacc.).
abstract: Sclerotium rolfsii (Sacc.) is a serious plant pathogenic fungus and lacks perfect (basidial) stage in production. Protoplast fusion technology was employed to reconstruct fusants from this fungus. Two strains designated as A and R were used. Maximum protoplast yields of 3.8x10(5)/g mycelia and 2.8x10(5)/g mycelia were formed in strains A and R respectively. Osmotic stabilizer sucrose 1M gave maximum yield. Lysing enzyme at the rate of 15mg/ml was found best for yield. Fusion of protoplasts from strains A and R was carried out in fusion media containing PEG 4000 30% (w/v) with 0.2mM CaCl2. Four fusants F1, F2, F3 and F4 were recovered. Morphological, physiological and pathogenic characters of fusants were compared with parent strains on carrots, beans and tomato.
authors.affiliation: Post-Doctoral Fellow Alberta Research Council , Vegreville, Alberta , Canada.
authors.forename: Yun
authors.initials: Y
authors.lastname: Cai
authors.forename: Christos
authors.initials: C
authors.lastname: Christias
keywords: Pathogenicity; Protoplast; Sclerotium rolfsii; fusion; osmotic stabilizer
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