title: Simulated microgravity combined with polyglycolic acid scaffold culture conditions improves the function of pancreatic islets.
abstract: The in vitro culture of pancreatic islets reduces their immunogenicity and prolongs their availability for transplantation. Both simulated microgravity (sMG) and a polyglycolic acid scaffold (PGA) are believed to confer advantages to cell culture. Here, we evaluated the effects of sMG combined with a PGA on the viability, insulin-producing activity and morphological alterations of pancreatic islets. Under PGA-sMG conditions, the purity of the islets was ≥85%, and the islets had a higher survival rate and an increased ability to secrete insulin compared with islets cultured alone in the static, sMG, or PGA conditions. In addition, morphological analysis under scanning electron microscopy (SEM) revealed that the PGA-sMG treatment preserved the integral structure of the islets and facilitated islet adhesion to the scaffolds. These results suggest that PGA-sMG coculture has the potential to improve the viability and function of islets in vitro and provides a promising method for islet transplantation.
authors.affiliation: Department of General Surgery, Peking Union Medical College Hospital, Beijing 100730, China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Song
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wei
authors.forename: Chun
authors.initials: C
authors.lastname: Song
authors.forename: Shanshan
authors.initials: S
authors.lastname: Xie
authors.forename: Jinfa
authors.initials: J
authors.lastname: Feng
authors.forename: Jiehou
authors.initials: J
authors.lastname: Fan
authors.forename: Zengling
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yubo
authors.initials: Y
authors.lastname: Shi
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D018920:Coculture Techniques; D006801:Humans; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000648:ultrastructure*; D016381:Islets of Langerhans Transplantation; D008855:Microscopy, Electron, Scanning; D011100:Polyglycolic Acid / Q000008:administration & dosage*; D051381:Rats; D018474:Weightlessness Simulation
chemical_list: D007328:Insulin; D011100:Polyglycolic Acid
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