title: Molecular taxonomy of the two Leishmania vectors Lutzomyia umbratilis and Lutzomyia anduzei (Diptera: Psychodidae) from the Brazilian Amazon.
abstract: BACKGROUND
  Lutzomyia umbratilis (a probable species complex) is the main vector of Leishmania guyanensis in the northern region of Brazil. Lutzomyia anduzei has been implicated as a secondary vector of this parasite. These species are closely related and exhibit high morphological similarity in the adult stage; therefore, they have been wrongly identified, both in the past and in the present. This shows the need for employing integrated taxonomy.
  
  
  METHODS
  With the aim of gathering information on the molecular taxonomy and evolutionary relationships of these two vectors, 118 sequences of 663 base pairs (barcode region of the mitochondrial DNA cytochrome oxidase I - COI) were generated from 72 L. umbratilis and 46 L. anduzei individuals captured, respectively, in six and five localities of the Brazilian Amazon. The efficiency of the barcode region to differentiate the L. umbratilis lineages I and II was also evaluated. The data were analyzed using the pairwise genetic distances matrix and the Neighbor-Joining (NJ) tree, both based on the Kimura Two Parameter (K2P) evolutionary model.
  
  
  RESULTS
  The analyses resulted in 67 haplotypes: 32 for L. umbratilis and 35 for L. anduzei. The mean intra-specific genetic distance was 0.008 (0.002 to 0.010 for L. umbratilis; 0.008 to 0.014 for L. anduzei), whereas the mean interspecific genetic distance was 0.044 (0.041 to 0.046), supporting the barcoding gap. Between the L. umbratilis lineages I and II, it was 0.009 to 0.010. The NJ tree analysis strongly supported monophyletic clades for both L. umbratilis and L. anduzei, whereas the L. umbratilis lineages I and II formed two poorly supported monophyletic subclades.
  
  
  CONCLUSIONS
  The barcode region clearly separated the two species and may therefore constitute a valuable tool in the identification of the sand fly vectors of Leishmania in endemic leishmaniasis areas. However, the barcode region had not enough power to separate the two lineages of L. umbratilis, likely reflecting incipient species that have not yet reached the status of distinct species.
authors.affiliation: Laboratório de Genética de Populações e Evolução de Mosquitos Vetores, Coordenação de Biodiversidade, Instituto Nacional de Pesquisas da Amazônia, Avenida André Araujo, 2,936, Bairro Petrópolis, Manaus CEP 69,067-375, Amazonas, Brazil. vera@inpa.gov.br.
authors.forename: Vera Margarete
authors.initials: VM
authors.lastname: Scarpassa
authors.forename: Ronildo Baiatone
authors.initials: RB
authors.lastname: Alencar
mesh_terms: D000818:Animals; D001938:Brazil; D004199:Disease Vectors; D006239:Haplotypes; D018334:Leishmania guyanensis / Q000302:isolation & purification; D008853:Microscopy; D008969:Molecular Sequence Data; D010802:Phylogeny; D011576:Psychodidae / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
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