title: Internal ribosome entry segment activity of ATXN8 opposite strand RNA.
abstract: Spinocerebellar ataxia type 8 (SCA8) involves the expansion of CTG/CAG repeats from the overlapping ataxin 8 opposite strand (ATXN8OS) and ataxin 8 (ATXN8) genes located on chromosome 13q21. Although being transcribed, spliced and polyadenylated in the CTG orientation, ATXN8OS does not itself appear to be protein coding, as only small open reading frames (ORFs) were noted. In the present study we investigated the translation of a novel 102 amino acids containing-ORF in the ATXN8OS RNA. Expression of chimeric construct with an in-frame ORF-EGFP gene demonstrated that ATXN8OS RNA is translatable. Using antiserum raised against ORF, ATXN8OS ORF expression was detected in various human cells including lymphoblastoid, embryonic kidney 293, neuroblastoma IMR-32, SK-N-SH, SH-SY5Y cells and human muscle tissue. The biological role of the ATXN8OS ORF and its connection to SCA8 remains to be determined.
authors.affiliation: Department of Life Science, National Taiwan Normal University, Taipei, Taiwan ; Department of Neurology, Chang Gung Memorial Hospital, Chang-Gung University College of Medicine, Taipei, Taiwan.
authors.forename: I-Cheng
authors.initials: IC
authors.lastname: Chen
authors.forename: Hsuan-Yuan
authors.initials: HY
authors.lastname: Lin
authors.forename: Ya-Chin
authors.initials: YC
authors.lastname: Hsiao
authors.forename: Chiung-Mei
authors.initials: CM
authors.lastname: Chen
authors.forename: Yih-Ru
authors.initials: YR
authors.lastname: Wu
authors.forename: Hsin-Chieh
authors.initials: HC
authors.lastname: Shiau
authors.forename: Yu-Fang
authors.initials: YF
authors.lastname: Shen
authors.forename: Kuo-Shiu
authors.initials: KS
authors.lastname: Huang
authors.forename: Ming-Tsan
authors.initials: MT
authors.lastname: Su
authors.forename: Hsiu-Mei
authors.initials: HM
authors.lastname: Hsieh-Li
authors.forename: Guey-Jen
authors.initials: GJ
authors.lastname: Lee-Chen
mesh_terms: D002460:Cell Line; D015870:Gene Expression; D023061:Gene Order; D006801:Humans; D016366:Open Reading Frames; D012313:RNA / Q000235:genetics* / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: C415906:ATXN8OS gene product, human; D062085:RNA, Long Noncoding; D011993:Recombinant Fusion Proteins; D012313:RNA
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