title: Intestinal lamina propria dendritic cells maintain T cell homeostasis but do not affect commensalism.
abstract: Dendritic cells (DCs) in the intestinal lamina propria (LP) are composed of two CD103(+) subsets that differ in CD11b expression. We report here that Langerin is expressed by human LP DCs and that transgenic human langerin drives expression in CD103(+)CD11b(+) LP DCs in mice. This subset was ablated in huLangerin-DTA mice, resulting in reduced LP Th17 cells without affecting Th1 or T reg cells. Notably, cognate DC-T cell interactions were not required for Th17 development, as this response was intact in huLangerin-Cre I-Aβ(fl/fl) mice. In contrast, responses to intestinal infection or flagellin administration were unaffected by the absence of CD103(+)CD11b(+) DCs. huLangerin-DTA x BatF3(-/-) mice lacked both CD103(+) LP DC subsets, resulting in defective gut homing and fewer LP T reg cells. Despite these defects in LP DCs and resident T cells, we did not observe alterations of intestinal microbial communities. Thus, CD103(+) LP DC subsets control T cell homeostasis through both nonredundant and overlapping mechanisms.
authors.affiliation: Department of Dermatology, Center for Immunology and 2 Department of Soil, Water, and Climate, Biotechnology Institute, University of Minnesota, Minneapolis, MN 55455.
authors.forename: Nathan E
authors.initials: NE
authors.lastname: Welty
authors.forename: Christopher
authors.initials: C
authors.lastname: Staley
authors.forename: Nico
authors.initials: N
authors.lastname: Ghilardi
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Sadowsky
authors.forename: Botond Z
authors.initials: BZ
authors.lastname: Igyártó
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Kaplan
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D039481:CD11b Antigen / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D004167:Diphtheria Toxin / Q000235:genetics; D006706:Homeostasis / Q000235:genetics / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D039001:Integrin alpha Chains / Q000378:metabolism; D007413:Intestinal Mucosa / Q000276:immunology* / Q000382:microbiology*; D037181:Lectins, C-Type / Q000235:genetics; D037241:Mannose-Binding Lectins / Q000235:genetics; D054892:Metagenome; D051379:Mice; D008822:Mice, Transgenic; D010641:Phenotype; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism; D058504:Th17 Cells / Q000276:immunology / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D039481:CD11b Antigen; C121916:CD207 protein, human; D004167:Diphtheria Toxin; D039001:Integrin alpha Chains; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins; C091367:alpha E integrins
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