title: A case of Diphyllobothrium nihonkaiense infection as confirmed by mitochondrial COX1 gene sequence analysis.
abstract: Diphyllobothrium nihonkaiense has been reported in Korea as Diphyllobothrium latum because of their close morphologic resemblance. We have identified a human case of D. nihonkaiense infection using the mitochondrial cytochrome c oxidase subunit I (cox1) gene sequence analysis. On 18 February 2012, a patient who had consumed raw fish a month earlier visited our outpatient clinic with a long tapeworm parasite excreted in the feces. The body of the segmented worm was 2 m long and divided into the scolex (head) and proglottids. It was morphologically close to D. nihonkaiense and D. latum. The cox1 gene analysis showed 99.4% (340/342 bp) homology with D. nihonkaiense but only 91.8% (314/342 bp) homology with D. latum. The present study suggested that the Diphyllobothrium spp. infection in Korea should be analyzed with specific DNA sequence for an accurate species identification.
authors.affiliation: Department of Internal Medicine, Chuncheon Sacred Heart Hospital, Chuncheon 200-704, Korea.
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Keeseon S
authors.initials: KS
authors.lastname: Eom
authors.forename: Min Sun
authors.initials: MS
authors.lastname: Park
authors.forename: Oh Kyoung
authors.initials: OK
authors.lastname: Kwon
authors.forename: Hyo Sun
authors.initials: HS
authors.lastname: Kim
authors.forename: Jai Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D000818:Animals; D051545:Cyclooxygenase 1 / Q000235:genetics*; D004169:Diphyllobothriasis / Q000469:parasitology*; D004170:Diphyllobothrium / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D015801:Helminth Proteins / Q000235:genetics*; D006801:Humans; D024101:Mitochondrial Proteins / Q000235:genetics*
chemical_list: D015801:Helminth Proteins; D024101:Mitochondrial Proteins; D051545:Cyclooxygenase 1
keywords: DNA; Diphyllobothrium latum; Diphyllobothrium nihonkaiense; cox1; diagnosis; sequence
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