title: Identification of Candida spp. by phenotypic tests and PCR.
abstract: The correct identification of Candida species is of great importance, as it presents prognostic and therapeutical significance, allowing an early and appropriate antifungical therapy. The purpose of this study was to identify isolates of Candida spp. from oral mucosa of 38 patients with oral candidosis evaluated in 2004 by phenotypic methods and PCR, discriminating C. albicans from the other Candida species. The tests used for phenotypic analysis were germ-tube and chlamydoconidia production, culture in CHROMAgar™ Candida, carbohydrate assimilation test, growth at 45ºC and culture in Tween 80 agar. Genotypic confirmation was performed by PCR. Phenotypic tests showed that 63.2% strains formed germ-tubes, 73.7% produced chlamydoconidia, and 63.2% showed green colonies in chromogenic medium, presumptively indicating C. albicans or C. dubliniensis. The carbohydrate assimilation test confirmed these results. A total of 21% strains were identified as C. krusei and 13.2% were indicative of C. tropicalis. Of these later strains, three produced chlamydoconidia. The association of other phenotypic tests with culture in Tween 80 agar identified 95.8% of strains as C. albicans and 4.2% as C. dubliniensis. All 24 strains indicative of C. albicans and C. dubliniensis were confirmed by PCR as C. albicans.
authors.affiliation: Laboratório de Imunologia e Microbiologia, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul , Porto Alegre, RS , Brasil ; Serviço de Estomatologia, Hospital São Lucas, Pontifícia Universidade Católica do Rio Grande do Sul , Porto Alegre, RS , Brasil.
authors.forename: Sandra Aparecida
authors.initials: SA
authors.lastname: Marinho
authors.forename: Alice Becker
authors.initials: AB
authors.lastname: Teixeira
authors.forename: Otávio Silveira
authors.initials: OS
authors.lastname: Santos
authors.forename: Ricardo Flores
authors.initials: RF
authors.lastname: Cazanova
authors.forename: Carlos Alexandre Sanchez
authors.initials: CA
authors.lastname: Ferreira
authors.forename: Karen
authors.initials: K
authors.lastname: Cherubini
authors.forename: Sílvia Dias
authors.initials: SD
authors.lastname: de Oliveira
keywords: Candida spp.; – identification – PCR – phenotypic tests
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