title: Migration of glial cells differentiated from neurosphere-forming neural stem/progenitor cells depends on the stiffness of the chemically cross-linked collagen gel substrate.
abstract: Substrate stiffness affects cell migration and spreading. Our study revealed that the stiffness of the cell-adhesive substrate affected the migration pattern of neural cells. We observed the migration of neural cells differentiated from neurosphere-forming neural stem/progenitor cells (NSPCs) on collagen gels with various degrees of stiffness achieved by chemical cross-linking. Both glial and neuronal cells broadly spread and migrated when stiff collagen gels (G'=5.5kPa, G″=0.2kPa) were used as the substrate. In contrast, the migration of glial cells was suppressed within the limited area on the soft collagen gels (G'=0.8kPa, G″=0.2kPa). Filopodia were rarely observed in glial cells on the soft collagen gels. Analysis of the intercellular distance between the closest neural cells after differentiation from NSPCs indicated that glial cells more broadly spread on the stiff collagen gels than on the soft gels. Immunocytochemical analysis showed that most of the migrated cells were glial cells, suggesting that migration of glial cells was dependent on the stiffness of substrate.
authors.affiliation: Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan. Electronic address: morihide@b.s.osakafu-u.ac.jp.
authors.forename: Hideki
authors.initials: H
authors.lastname: Mori
authors.forename: Ayumi
authors.initials: A
authors.lastname: Takahashi
authors.forename: Ayano
authors.initials: A
authors.lastname: Horimoto
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hara
mesh_terms: D000818:Animals; D002234:Carbodiimides / Q000737:chemistry; D002454:Cell Differentiation; D002465:Cell Movement; D002540:Cerebral Cortex / Q000166:cytology; D024042:Collagen Type I / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D053595:Embryonic Stem Cells / Q000166:cytology*; D005782:Gels; D051379:Mice; D008813:Mice, Inbred ICR; D058953:Neural Stem Cells / Q000166:cytology*; D009457:Neuroglia / Q000166:cytology / Q000502:physiology*
chemical_list: C518491:1-ethyl-3-(3-(diethylamino)propyl)carbodiimide; D002234:Carbodiimides; D024042:Collagen Type I; D003432:Cross-Linking Reagents; D005782:Gels
keywords: Cell migration; Collagen gel; Neural stem cell; Neurosphere; Stiffness