title: An in-house multiplex pcr method to detect of putative virulence factors in aeromonas species.
abstract: A pentaplex PCR was developed and optimised to detect the genes that encode the five most important putative virulence factors in Aeromonas isolates. It seems to be more efficient than previously reported techniques and promises to be a powerful tool for more accurate risk assessments and for monitoring pathogenic strains.
authors.affiliation: Escuela Nacional de Ciencias Biológicas , Instituto Politécnico Nacional , Mexico, D.F.
authors.forename: Ma Guadalupe
authors.initials: MG
authors.lastname: Aguilera-Arreola
authors.forename: Alma Aidee Carmona
authors.initials: AA
authors.lastname: Martínez
authors.forename: Graciela
authors.initials: G
authors.lastname: Castro-Escarpulli
keywords: Aeromonas; pathogenesis; pentaplex-PCR; virulence potential
references.citation: Int J Syst Evol Microbiol. 2000 Nov;50 Pt 6:2069-2073
references.pmid: 11155981
references.citation: Appl Environ Microbiol. 2010 Jan;76(2):425-33
references.pmid: 19933350
references.citation: J Food Prot. 2008 Oct;71(10):2094-9
references.pmid: 18939759
references.citation: Syst Appl Microbiol. 2010 Jan;33(1):15-9
references.pmid: 20005654
references.citation: Antonie Van Leeuwenhoek. 2003;84(4):269-78
references.pmid: 14574104
references.citation: Syst Appl Microbiol. 2009 Oct;32(7):471-9
references.pmid: 19570633
references.citation: Antonie Van Leeuwenhoek. 2009 Jun;96(1):53-61
references.pmid: 19347601
references.citation: Int J Syst Evol Microbiol. 2011 Feb;61(Pt 2):242-248
references.pmid: 20207806
references.citation: Can J Microbiol. 2007 Feb;53(2):261-9
references.pmid: 17496975
references.citation: J Appl Microbiol. 2004;97(5):1077-86
references.pmid: 15479425
references.citation: J Fish Dis. 2005 Jul;28(7):437-41
references.pmid: 16083449
references.citation: Microb Pathog. 2007 May-Jun;42(5-6):193-203
references.pmid: 17368824
references.citation: Clin Microbiol Rev. 2010 Jan;23(1):35-73
references.pmid: 20065325
references.citation: PCR Methods Appl. 1994 Feb;3(4):S65-75
references.pmid: 8173510
references.citation: Diagn Microbiol Infect Dis. 2002 Nov;44(3):221-5
references.pmid: 12493167
references.citation: Int J Syst Evol Microbiol. 2010 Jan;60(Pt 1):72-77
references.pmid: 19648345
references.citation: Appl Environ Microbiol. 1999 Dec;65(12):5293-302
references.pmid: 10583979
references.citation: FEMS Microbiol Lett. 2005 Jan 15;242(2):231-40
references.pmid: 15621443
references.citation: Can J Microbiol. 2007 Jul;53(7):877-87
references.pmid: 17898843
references.citation: Int J Food Microbiol. 2003 Jul 15;84(1):41-9
references.pmid: 12781953
references.citation: J Microbiol. 2007 Aug;45(4):297-304
references.pmid: 17846582
references.citation: J Clin Microbiol. 1997 Jul;35(7):1671-4
references.pmid: 9196171
references.citation: Int J Syst Evol Microbiol. 2010 Sep;60(Pt 9):2048-2055
references.pmid: 19819994
references.citation: J Appl Microbiol. 2002;92(2):221-7
references.pmid: 11849349
references.citation: Clin Microbiol Rev. 2000 Oct;13(4):559-70
references.pmid: 11023957
references.citation: Environ Microbiol. 2004 Mar;6(3):198-208
references.pmid: 14871204
references.citation: Mol Microbiol. 2002 Jan;43(2):383-97
references.pmid: 11985716
references.citation: Infect Immun. 2002 Apr;70(4):1924-35
references.pmid: 11895956
references.citation: FEMS Immunol Med Microbiol. 2002 Feb 18;32(3):243-7
references.pmid: 11934570