title: Characterization and expression profiling of cucumber kinesin genes during early fruit development: revealing the roles of kinesins in exponential cell production and enlargement in cucumber fruit.
abstract: Rapid cell division and expansion in early fruit development are important phases for cucumber fruit yield and quality. Kinesin proteins are microtubule-based motors responsible for modulating cell division and enlargement. In this work, the candidate kinesin genes involved in rapid cell division and expansion during cucumber fruit development were investigated. The morphological and cellular changes during early fruit development were compared in four cucumber genotypes with varied fruit size. The correlation between the expression profiles of cucumber kinesin genes and cellular changes in fruit was investigated. Finally, the biochemical characteristics and subcellular localizations of three candidate kinesins were studied. The results clarified the morphological and cellular changes during early cucumber fruit development. This study found that CsKF2-CsKF6 were positively correlated with rapid cell production; CsKF1 and CsKF7 showed a strongly positive correlation with rapid cell expansion. The results also indicated that CsKF1 localized to the plasma membrane of fast-expanding fruit cells, that CsKF2 might play a role in fruit chloroplast division, and that CsKF3 is involved in the function or formation of phragmoplasts in fruit telophase cells. The results strongly suggest that specific fruit-enriched kinesins are specialized in their functions in rapid cell division and expansion during cucumber fruit development.
authors.affiliation: Institute of Vegetables and Flowers (IVF), the Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
authors.forename: Xue Yong
authors.initials: XY
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Wei Jie
authors.initials: WJ
authors.lastname: Jiang
authors.forename: Xiao Ling
authors.initials: XL
authors.lastname: Liu
authors.forename: Xiao Meng
authors.initials: XM
authors.lastname: Zhang
authors.forename: Hong Jun
authors.initials: HJ
authors.lastname: Yu
authors.forename: San Wen
authors.initials: SW
authors.lastname: Huang
authors.forename: Guo Qin
authors.initials: GQ
authors.lastname: Liu
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D017360:Arabidopsis / Q000378:metabolism; D002455:Cell Division / Q000235:genetics; D049109:Cell Proliferation; D048429:Cell Size; D003001:Cloning, Molecular; D016000:Cluster Analysis; D018553:Cucumis sativus / Q000033:anatomy & histology / Q000166:cytology* / Q000235:genetics* / Q000254:growth & development; D005638:Fruit / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D015151:Immunoblotting; D016547:Kinesins / Q000235:genetics* / Q000378:metabolism; D009929:Organ Size / Q000235:genetics; D059828:Plant Cells / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011523:Protoplasts / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D000251:Adenosine Triphosphatases; D016547:Kinesins
keywords: Cell division; chloroplast; cucumber; expansion; fruit; kinesin; phragmoplast; plasma membrane.
references.citation: Anal Biochem. 1978 Mar;85(1):86-9
references.pmid: 147037
references.citation: Trends Plant Sci. 2002 Mar;7(3):103-5
references.pmid: 11906832
references.citation: Plant Cell. 1998 Dec;10(12):1991-2004
references.pmid: 9836740
references.citation: J Struct Biol. 1998 Dec 15;124(2-3):303-10
references.pmid: 10049813
references.citation: Plant J. 2004 Sep;39(5):697-714
references.pmid: 15315633
references.citation: Plant Physiol. 2005 Oct;139(2):750-69
references.pmid: 16183847
references.citation: Curr Biol. 2000 Jun 29;10(13):797-800
references.pmid: 10898978
references.citation: Protoplasma. 2008;232(3-4):255-65
references.pmid: 18421552
references.citation: Annu Rev Cell Dev Biol. 1997;13:745-77
references.pmid: 9442886
references.citation: Planta. 2004 Nov;220(1):156-64
references.pmid: 15258761
references.citation: Trends Plant Sci. 2006 Apr;11(4):167-75
references.pmid: 16530461
references.citation: FEBS Lett. 1998 Jul 3;430(3):278-82
references.pmid: 9688555
references.citation: J Exp Bot. 2011 Jan;62(1):205-19
references.pmid: 20732881
references.citation: Protoplasma. 2012 Jun;249 Suppl 2:S95-100
references.pmid: 22167300
references.citation: J Exp Bot. 2008;59(9):2299-308
references.pmid: 18515830
references.citation: Plant Cell. 2011 Jan;23(1):111-29
references.pmid: 21282525
references.citation: BMC Genomics. 2012 Oct 02;13:518
references.pmid: 23031452
references.citation: Biochem J. 2009 Jun 26;421(2):171-80
references.pmid: 19416090
references.citation: Mol Biol Cell. 2005 Feb;16(2):811-23
references.pmid: 15574882
references.citation: Plant Cell. 1993 Oct;5(10):1439-1451
references.pmid: 12271039
references.citation: EMBO J. 1991 Jan;10(1):183-93
references.pmid: 1989883
references.citation: Nat Rev Mol Cell Biol. 2009 Oct;10(10):682-96
references.pmid: 19773780
references.citation: Plant Biol (Stuttg). 2010 Jan;12(1):98-107
references.pmid: 20653892
references.citation: Plant Cell Physiol. 2010 Aug;51(8):1315-29
references.pmid: 20587735
references.citation: Nat Protoc. 2007;2(7):1565-72
references.pmid: 17585298
references.citation: Plant Physiol. 2009 Mar;149(3):1505-28
references.pmid: 19144766
references.citation: Plant Cell. 2007 Jan;19(1):226-36
references.pmid: 17220198
references.citation: Plant Physiol. 1997 Jun;114(2):557-564
references.pmid: 12223728
references.citation: Development. 2002 May;129(10):2401-9
references.pmid: 11973272
references.citation: Plant Cell. 2011 Feb;23(2):628-40
references.pmid: 21325138
references.citation: Nat Genet. 2009 Dec;41(12):1275-81
references.pmid: 19881527
references.citation: Plant Cell. 2001 Nov;13(11):2427-39
references.pmid: 11701879
references.citation: J Cell Sci. 2007 Aug 15;120(Pt 16):2819-27
references.pmid: 17652157
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: BMC Plant Biol. 2008 Feb 17;8:16
references.pmid: 18279528
references.citation: Protoplasma. 2012 Oct;249(4):887-99
references.pmid: 22038119
references.citation: Annu Rev Cell Biol. 1986;2:421-57
references.pmid: 3548773
references.citation: Curr Biol. 2006 May 9;16(9):888-94
references.pmid: 16682350
references.citation: Proc Natl Acad Sci U S A. 2011 Oct 25;108(43):17844-9
references.pmid: 22006334
references.citation: Plant Physiol. 2004 Dec;136(4):3877-83
references.pmid: 15591445