title: In situ recognition of cell-surface glycans and targeted imaging of cancer cells.
abstract: Fluorescent sensors capable of recognizing cancer-associated glycans, such as sialyl Lewis X (sLe(x)) tetrasaccharide, have great potential for cancer diagnosis and therapy. Studies on water-soluble and biocompatible sensors for in situ recognition of cancer-associated glycans in live cells and targeted imaging of cancer cells are very limited at present. Here we report boronic acid-functionalized peptide-based fluorescent sensors (BPFSs) for in situ recognition and differentiation of cancer-associated glycans, as well as targeted imaging of cancer cells. By screening BPFSs with different structures, it was demonstrated that BPFS₁ with a FRGDF peptide could recognize cell-surface glycan of sLe(x) with high specificity and thereafter fluorescently label and discriminate cancer cells through the cooperation with the specific recognition between RGD and integrins. The newly developed peptide-based sensor will find great potential as a fluorescent probe for cancer diagnosis.
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China.
authors.forename: Xiao-Ding
authors.initials: XD
authors.lastname: Xu
authors.forename: Han
authors.initials: H
authors.lastname: Cheng
authors.forename: Wei-Hai
authors.initials: WH
authors.lastname: Chen
authors.forename: Si-Xue
authors.initials: SX
authors.lastname: Cheng
authors.forename: Ren-Xi
authors.initials: RX
authors.lastname: Zhuo
authors.forename: Xian-Zheng
authors.initials: XZ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D016023:Integrins / Q000378:metabolism; D008024:Ligands; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging; D015394:Molecular Structure; D009369:Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D000080506:Sialyl Lewis X Antigen
chemical_list: D005456:Fluorescent Dyes; D016023:Integrins; D008024:Ligands; D009844:Oligosaccharides; D011134:Polysaccharides; D000080506:Sialyl Lewis X Antigen
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