title: Rapid and sensitive immunochromatographic strip for on-site detection of sulfamethazine in meats and eggs.
abstract: A rapid immunochromatographic (ICG) strip based on a conjugate of colloidal gold and monoclonal antibody (mAb) was developed for the rapid, sensitive, and on-site detection of sulfamethazine in meat and egg samples. The detection limit of the ICG strip is 2 ng/mL, and the assay can be completed in 10 min. A cross-reactivity test indicated that the ICG strip was highly specific to sulfamethazine with no cross-reaction with sulfonamide compounds and other antibiotics. The results of the recovery test from meat and egg samples spiked with sulfamethazine were in good agreement with those obtained by the indirect competitive enzyme-linked immunosorbent assay. These results demonstrated that the ICG strip can be used as a rapid and qualitative tool for on-site screening of sulfamethazine in meat and egg samples.
authors.affiliation: School of Physics and Chemistry, Gwangju Inst. of Science and Technology, Gwangju, 500-712, Republic of Korea.
authors.forename: Won-Bo
authors.initials: WB
authors.lastname: Shim
authors.affiliation: Inst. of Agriculture and Life Science, Gyeongsang Natl. Univ., Jinju, Gyeongnam 660-701, Republic of Korea.
authors.forename: Jeong-Sook
authors.initials: JS
authors.lastname: Kim
authors.affiliation: School of Physics and Chemistry, Gwangju Inst. of Science and Technology, Gwangju, 500-712, Republic of Korea.
authors.forename: Min-Gon
authors.initials: MG
authors.lastname: Kim
authors.affiliation: Inst. of Agriculture and Life Science, Gyeongsang Natl. Univ., Jinju, Gyeongnam 660-701, Republic of Korea.|Div. of Applied Life Science, Graduate School, Gyeongsang Natl. Univ., Jinju, Gyeongnam 660-701, Republic of Korea.
authors.forename: Duck-Hwa
authors.initials: DH
authors.lastname: Chung
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal / Q000378:metabolism; D002417:Cattle; D002645:Chickens; D002846:Chromatography, Affinity / Q000379:methods*; D004531:Eggs / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013418:Sulfamethazine / Q000302:isolation & purification*; D013552:Swine
chemical_list: D000890:Anti-Infective Agents; D000911:Antibodies, Monoclonal; D013418:Sulfamethazine
keywords: immunochromatographic strip; monoclonal antibody; rapid detection; sulfamethazine