title: Identification and characterization of the endophytic plant growth prompter Bacillus Cereus strain mq23 isolated from Sophora Alopecuroides root nodules.
abstract: Endophytes MQ23 and MQ23R isolated from Sophora alopecuroides root nodules were characterized by observing their ability to promote plant growth and employing molecular analysis techniques. Results showed that MQ23 and MQ23R are potential N2-fixing endophytes and belong to the same species as Bacillus cereus. MQ23 was shown to be able to produce siderophores, IAA, and demonstrate certain antifungal activity to plant pathogenic fungi. Co-inoculation with MQ23+MQ23II showed a more significant effect than inoculation alone in vitro for most of positive actions suggesting they have a cooperative interaction. Results of plant inoculation with endophytes indicated that the growth indexes of co-inoculated MQ23+MQ23II were higher than those of inoculated alone (p<0.05) (the exception being for root fresh weight) when compared to negative control. There have been little of any studies of nonrhizobial putative endophytes with growth-promotion attributes in S. alopecuroides root nodules. This could be exploited as potential bio-inoculants and biocontrol agents in agriculture.
authors.affiliation: College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University , Yangling, Shaanxi, 712100 , China ; College of Life Sciences, Shangqiu Normal University , Shangqiu, Henan, 476000 , China.
authors.forename: Longfei
authors.initials: L
authors.lastname: Zhao
authors.forename: Yajun
authors.initials: Y
authors.lastname: Xu
authors.forename: Ran
authors.initials: R
authors.lastname: Sun
authors.forename: Zhenshan
authors.initials: Z
authors.lastname: Deng
authors.forename: Wenquan
authors.initials: W
authors.lastname: Yang
authors.forename: Gehong
authors.initials: G
authors.lastname: Wei
keywords: Bacillus cereus; Endophytic bacteria; Plant growth promoting characterization; Root nodules; Sophora alopecuroides
references.citation: Int J Syst Evol Microbiol. 2005 Mar;55(Pt 2):569-575
references.pmid: 15774626
references.citation: Nature. 1970 Aug 15;227(5259):680-5
references.pmid: 5432063
references.citation: Can J Microbiol. 2000 Jul;46(7):600-6
references.pmid: 10932352
references.citation: Biosci Biotechnol Biochem. 2009 Jul;73(7):1489-93
references.pmid: 19584554
references.citation: Mol Biol Evol. 1987 Jul;4(4):406-25
references.pmid: 3447015
references.citation: Bioresour Technol. 2008 May;99(8):3016-23
references.pmid: 17706411
references.citation: Microb Ecol. 2006 Oct;52(3):436-43
references.pmid: 16897296
references.citation: Anal Biochem. 1987 Jan;160(1):47-56
references.pmid: 2952030
references.citation: Int J Syst Bacteriol. 1997 Jul;47(3):874-9
references.pmid: 9226921
references.citation: Can J Microbiol. 1959 Feb;5(1):79-85
references.pmid: 13629388
references.citation: Int J Syst Bacteriol. 1999 Jan;49 Pt 1:51-65
references.pmid: 10028247
references.citation: FEMS Microbiol Lett. 2008 Jan;278(1):1-9
references.pmid: 18034833
references.citation: J Exp Bot. 2005 Jul;56(417):1761-78
references.pmid: 15911555
references.citation: Plant Physiol. 1951 Jan;26(1):192-5
references.pmid: 16654351
references.citation: Int J Syst Bacteriol. 1996 Jan;46(1):240-4
references.pmid: 8573502
references.citation: Bioresour Technol. 2008 Jul;99(11):4544-50
references.pmid: 17826983
references.citation: Syst Appl Microbiol. 2005 Jul;28(5):465-77
references.pmid: 16094873
references.citation: J Appl Microbiol. 2005;99(5):1271-81
references.pmid: 16238759
references.citation: FEMS Microbiol Lett. 2006 Jan;254(2):258-67
references.pmid: 16445754
references.citation: Appl Environ Microbiol. 1993 Jul;59(7):2190-6
references.pmid: 8357252
references.citation: Mol Phylogenet Evol. 2004 Mar;30(3):720-32
references.pmid: 15012950
references.citation: Mikrobiologiia. 2003 Jan-Feb;72(1):54-9
references.pmid: 12698792
references.citation: Curr Microbiol. 2008 Feb;56(2):134-9
references.pmid: 18074178
references.citation: Arch Microbiol. 2010 Mar;192(3):229-34
references.pmid: 20098981
references.citation: Syst Appl Microbiol. 2010 Dec;33(8):468-77
references.pmid: 20965680
references.citation: Res Microbiol. 2001 Jan-Feb;152(1):95-103
references.pmid: 11281330