title: Differentiation of bovine coronavirus (bcov) genotypes by a restriction enzyme assay.
abstract: This article reports the use of the GsuI restriction enzyme to differentiate genotypes of Bovine Coronavirus (BCoV), based on an 18-nucleotide deletion of S1-coding region found in one of the two genotypes. It was concluded that this assay can be used as a rapid tool for BCoV genotypes differentiation.
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo , São Paulo, SP , Brasil ; Grupo de Pesquisa Coronavirus , São Paulo, SP , Brasil.
authors.forename: Sibele
authors.initials: S
authors.lastname: Pinheiro de Souza
authors.forename: Karen
authors.initials: K
authors.lastname: Miyuki Asano
authors.forename: Thaisa
authors.initials: T
authors.lastname: Lucas Sandri
authors.forename: Iracema
authors.initials: I
authors.lastname: Nunes de Barros
authors.forename: Leonardo
authors.initials: L
authors.lastname: José Richtzenhain
authors.forename: Paulo
authors.initials: P
authors.lastname: Eduardo Brandão
keywords: Bovine coronavirus; gene S; genotypes; restriction enzyme
references.citation: Arch Virol. 2006 Sep;151(9):1735-48
references.pmid: 16583154
references.citation: Adv Virus Res. 2006;66:193-292
references.pmid: 16877062
references.citation: J Vet Med Sci. 2009 Jan;71(1):83-6
references.pmid: 19194080
references.citation: Virology. 1982 Jun;119(2):358-71
references.pmid: 6281979
references.citation: Arch Virol. 2003 Nov;148(11):2207-35
references.pmid: 14579179
references.citation: Zentralbl Veterinarmed B. 1999 Dec;46(10):693-700
references.pmid: 10676147
references.citation: J Gen Virol. 1995 May;76 ( Pt 5):1263-70
references.pmid: 7730812