title: Microarray chip based identification of a mixed infection of bovine herpesvirus 1 and bovine viral diarrhea 2 from Indian cattle.
abstract: Bovine herpesvirus 1 (BHV1) and bovine viral diarrhea virus 2 (BVD2) are endemic in India although no mixed infection with these viruses has been reported from India. We report first mixed infection of these viruses in cattle during routine screening with a microarray chip. 62 of the 69 probes of BHV1 and 42 of the 57 BVD2 probes in the chip gave positive signals for the virus. The virus infections were subsequently confirmed by RT-PCR. We also discuss the implications of these findings.
authors.affiliation: Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, 243122, India.
authors.forename: Barkha
authors.initials: B
authors.lastname: Ratta
authors.forename: Brijesh Singh
authors.initials: BS
authors.lastname: Yadav
authors.forename: Mayank
authors.initials: M
authors.lastname: Pokhriyal
authors.forename: Meeta
authors.initials: M
authors.lastname: Saxena
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Sharma
mesh_terms: D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000821:virology*; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D060085:Coinfection / Q000662:veterinary / Q000821:virology*; D030242:Diarrhea Virus 2, Bovine Viral / Q000235:genetics* / Q000302:isolation & purification; D007242:Herpesvirus 1, Bovine / Q000235:genetics* / Q000302:isolation & purification; D007194:India; D007241:Infectious Bovine Rhinotracheitis / Q000821:virology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D015203:Reproducibility of Results
chemical_list: D012367:RNA, Viral
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