title: Safety and efficient ex vivo expansion of stem cells using platelet-rich plasma technology.
abstract: The goal of this Review is to provide an overview of the cell culture media supplements used in the ex vivo expansion of stem cells intended for cell therapy. Currently, the gold standard is the culture supplemented with fetal bovine serum, however, their use in cell therapy raises many concerns. The alternatives to its use are presented, ranging from the use of human serum to platelet-rich plasma (PRP), to serum-free media or extracellular matrix components. Finally, various growth factors present in PRP are described, which make it a safe and effective stem cell expansion supplement. These growth factors could be responsible for their efficiency, as they increase both stem cell proliferation and survival. The different PRP formulations are also discussed, as well as the need for protocol standardization.
authors.affiliation: Eduardo Anitua. Instituto Eduardo Anitua; c/Jose Maria Cagigal 19, 01007 Vitoria, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Anitua
authors.forename: Roberto
authors.initials: R
authors.lastname: Prado
authors.forename: Gorka
authors.initials: G
authors.lastname: Orive
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D016895:Culture Media, Serum-Free; D005109:Extracellular Matrix / Q000502:physiology; D006801:Humans; D053657:Platelet-Rich Plasma; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*
chemical_list: D016895:Culture Media, Serum-Free