title: Genomic resources for gene discovery, functional genome annotation, and evolutionary studies of maize and its close relatives.
abstract: Maize is one of the most important food crops and a key model for genetics and developmental biology. A genetically anchored and high-quality draft genome sequence of maize inbred B73 has been obtained to serve as a reference sequence. To facilitate evolutionary studies in maize and its close relatives, much like the Oryza Map Alignment Project (OMAP) (www.OMAP.org) bacterial artificial chromosome (BAC) resource did for the rice community, we constructed BAC libraries for maize inbred lines Zheng58, Chang7-2, and Mo17 and maize wild relatives Zea mays ssp. parviglumis and Tripsacum dactyloides. Furthermore, to extend functional genomic studies to maize and sorghum, we also constructed binary BAC (BIBAC) libraries for the maize inbred B73 and the sorghum landrace Nengsi-1. The BAC/BIBAC vectors facilitate transfer of large intact DNA inserts from BAC clones to the BIBAC vector and functional complementation of large DNA fragments. These seven Zea Map Alignment Project (ZMAP) BAC/BIBAC libraries have average insert sizes ranging from 92 to 148 kb, organellar DNA from 0.17 to 2.3%, empty vector rates between 0.35 and 5.56%, and genome equivalents of 4.7- to 8.4-fold. The usefulness of the Parviglumis and Tripsacum BAC libraries was demonstrated by mapping clones to the reference genome. Novel genes and alleles present in these ZMAP libraries can now be used for functional complementation studies and positional or homology-based cloning of genes for translational genomics.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Xue
authors.initials: X
authors.lastname: Shi
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Haiyan
authors.initials: H
authors.lastname: Li
authors.forename: Jetty S S
authors.initials: JS
authors.lastname: Ammiraju
authors.forename: David A
authors.initials: DA
authors.lastname: Kudrna
authors.forename: Wentao
authors.initials: W
authors.lastname: Xiong
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Zhaozhao
authors.initials: Z
authors.lastname: Dai
authors.forename: Yonglian
authors.initials: Y
authors.lastname: Zheng
authors.forename: Jinsheng
authors.initials: J
authors.lastname: Lai
authors.forename: Weiwei
authors.initials: W
authors.lastname: Jin
authors.forename: Joachim
authors.initials: J
authors.lastname: Messing
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Bennetzen
authors.forename: Rod A
authors.initials: RA
authors.lastname: Wing
authors.forename: Meizhong
authors.initials: M
authors.lastname: Luo
mesh_terms: D022202:Chromosomes, Artificial, Bacterial; D019143:Evolution, Molecular; D015723:Gene Library; D018745:Genome, Plant; D023281:Genomics; D008969:Molecular Sequence Data; D006109:Poaceae / Q000235:genetics; D045868:Sorghum / Q000235:genetics; D003313:Zea mays / Q000235:genetics*
keywords: BAC; BIBAC; Tripsacum; maize; parviglumis
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