title: Short communication: In vitro antimicrobial susceptibility of Mycoplasma agalactiae strains isolated from dairy goats.
abstract: This study examined the susceptibility to several antimicrobials of 28 isolates of Mycoplasma agalactiae obtained from goats in a region (southeastern Spain) where contagious agalactia is endemic. For each isolate, the minimum inhibitory concentration (MIC) against 12 antimicrobials of the quinolone, macrolide, aminoglycoside, and tetracycline families was determined. The antimicrobials with the lowest MIC were enrofloxacin, ciprofloxacin, tylosin, and doxycycline, all with MIC90 (concentration at which growth of 90% of the isolates is inhibited) <1 µg/mL. Norfloxacin (a quinolone) showed a wide MIC range (0.1-12.8 µg/mL), suggesting a resistance mechanism toward this antimicrobial that was not elicited by enrofloxacin or ciprofloxacin (the other quinolones tested). Erythromycin showed the highest MIC90 such that its use against Mycoplasma agalactiae is not recommended. Finally, Mycoplasma agalactiae isolates obtained from goat herds with clinical symptoms of contagious agalactia featured higher MIC90 and MIC50 (concentration at which growth of 50% of the isolates is inhibited) values for many of the antimicrobials compared with isolates from asymptomatic animals. The relationship between the extensive use of antimicrobials in herds with clinical contagious agalactia and variations in MIC requires further study.
authors.affiliation: Research Group on Ruminant Health, Animal Health Department, School of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum," University of Murcia, 30071 Murcia, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Paterna
authors.affiliation: Research Group on Ruminant Health, Animal Health Department, School of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum," University of Murcia, 30071 Murcia, Spain. Electronic address: asanlope@um.es.
authors.forename: A
authors.initials: A
authors.lastname: Sánchez
authors.affiliation: Research Group on Ruminant Health, Animal Health Department, School of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum," University of Murcia, 30071 Murcia, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Gómez-Martín
authors.affiliation: Research Group on Ruminant Health, Animal Health Department, School of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum," University of Murcia, 30071 Murcia, Spain.
authors.forename: J C
authors.initials: JC
authors.lastname: Corrales
authors.affiliation: Research Group on Ruminant Health, Animal Health Department, School of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum," University of Murcia, 30071 Murcia, Spain.
authors.forename: C
authors.initials: C
authors.lastname: De la Fe
authors.affiliation: Research Group on Ruminant Health, Animal Health Department, School of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum," University of Murcia, 30071 Murcia, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Contreras
authors.affiliation: Research Group on Ruminant Health, Animal Health Department, School of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum," University of Murcia, 30071 Murcia, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Amores
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D000077422:Enrofloxacin; D004917:Erythromycin / Q000494:pharmacology; D005260:Female; D024841:Fluoroquinolones / Q000494:pharmacology; D015511:Goat Diseases / Q000188:drug therapy / Q000382:microbiology; D006041:Goats / Q000382:microbiology*; D018942:Macrolides / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D009175:Mycoplasma Infections / Q000188:drug therapy / Q000662:veterinary; D045644:Mycoplasma agalactiae / Q000187:drug effects*; D015363:Quinolones / Q000494:pharmacology; D013030:Spain; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D018942:Macrolides; D015363:Quinolones; D000077422:Enrofloxacin; D002939:Ciprofloxacin; D004917:Erythromycin; D013752:Tetracycline
keywords: Mycoplasma agalactiae; antimicrobial; contagious agalactia; minimum inhibitory concentration