title: Loop-mediated isothermal amplification method for a differential identification of human Taenia tapeworms.
abstract: Loop-mediated isothermal amplification (LAMP), which employs a Bst DNA polymerase with strand-displacement activity and four primers (two inner primers and two outer primers) recognizing six distinct regions on the target DNA, is a highly sensitive, specific, simple, and rapid nucleotide amplification method. Moreover, because the Bst DNA polymerase resists much DNA polymerase inhibitors present in biological specimens, the LAMP method is suitable for the detection of infectious agents from clinical material such as fecal samples. Here, we describe the LAMP method which can differentially detect and identify human Taenia tapeworms, Taenia solium, T. saginata, and T. asiatica, using DNA specimens prepared from parasite tissue and human fecal sample.
authors.affiliation: Department of Parasitology, Asahikawa Medical University, Asahikawa, Japan.
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Sako
authors.forename: Agathe
authors.initials: A
authors.lastname: Nkouawa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yanagida
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
mesh_terms: D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000235:genetics / Q000302:isolation & purification; D003937:Diagnosis, Differential; D005243:Feces / Q000469:parasitology; D017238:Genes, Helminth; D006801:Humans; D025202:Molecular Diagnostic Techniques; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D041202:Taenia saginata / Q000235:genetics*; D013622:Taeniasis / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D018509:DNA, Helminth