title: Effect of storage temperature on the microbial composition of ready-to-use vegetables.
abstract: Four different salad preparations were investigated from microbiological point of view: two were packaged in air and two under Modified Atmosphere. The samples were stored at 4 and 10 °C, and analysed at established times. Total bacterial count (TBC) was taken as the most relevant index to define their hygiene and quality at both temperatures. Lactic acid bacteria, yeasts and moulds were found only occasionally. In general, the most important factor was the packaging technique: TBC was lower when the product is packed under modified conditions. The packaging technique also influences the microbial population: Gram-negative aerobic rods are dominant in air-packaged products, whilst the presence of Enterobacteriaceae becomes important in salads packaged under Modified Atmosphere. Pseudomonas fluorescens, with all its biovars, was the most frequently found species amongst the aerobic isolates, whilst for the Enterobacteriaceae strains, there was no dominant species.
authors.affiliation: Department of Food, Environmental and Nutritional Sciences, DeFENS, Università degli Studi di Milano, via Celoria 2, 20133, Milan, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Caldera
authors.forename: L
authors.initials: L
authors.lastname: Franzetti
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D058491:Bacterial Load; D004269:DNA, Bacterial; D021901:DNA, Intergenic; D005516:Food Microbiology; D005519:Food Preservation; D006801:Humans; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013696:Temperature; D014675:Vegetables / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D021901:DNA, Intergenic; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
references.citation: FEMS Microbiol Lett. 2004 Jul 15;236(2):257-60
references.pmid: 15251205
references.citation: J Lab Clin Med. 1954 Aug;44(2):301-7
references.pmid: 13184240
references.citation: Microbiology (Reading). 2003 Mar;149(Pt 3):807-813
references.pmid: 12634348
references.citation: J Food Prot. 2001 Feb;64(2):228-34
references.pmid: 11271772
references.citation: Int J Syst Evol Microbiol. 2000 Jul;50 Pt 4:1629-1639
references.pmid: 10939670
references.citation: Int J Food Microbiol. 2008 Mar 31;123(1-2):121-9
references.pmid: 18237811
references.citation: Food Microbiol. 2007 Oct-Dec;24(7-8):711-7
references.pmid: 17613368
references.citation: Int J Food Microbiol. 2012 Jun 15;157(1):52-8
references.pmid: 22561064
references.citation: Int J Food Microbiol. 2005 Mar 1;99(1):71-7
references.pmid: 15718030
references.citation: Proc Natl Acad Sci U S A. 1985 Oct;82(20):6955-9
references.pmid: 2413450
references.citation: J Bacteriol. 1953 Jul;66(1):24-6
references.pmid: 13069461
references.citation: Microbiology (Reading). 2000 Aug;146 ( Pt 8):2027-2038
references.pmid: 10931907
references.citation: J Gen Microbiol. 1966 May;43(2):159-271
references.pmid: 5963505
references.citation: J Appl Bacteriol. 1966 Dec;29(3):470-89
references.pmid: 5980915
references.citation: J Appl Microbiol. 2010 Sep;109(3):996-1006
references.pmid: 20408920
references.citation: Food Microbiol. 2010 Dec;27(8):1071-7
references.pmid: 20832687
references.citation: Arch Latinoam Nutr. 2002 Sep;52(3):282-8
references.pmid: 12448343
references.citation: J Food Prot. 2007 May;70(5):1277-80
references.pmid: 17536694