title: A defective mutant of Salmonella enterica Serovar Gallinarum in cobalamin biosynthesis is avirulent in chickens.
abstract: Salmonella enterica serovar Gallinarum (SG) is a fowl typhoid agent in chickens and is a severe disease with worldwide economic impact as its mortality may reach up to 80%. It is one of a small group of serovars that typically produces typhoid-like infections in a narrow range of host species and which therefore represents a good model for human typhoid. The survival mechanisms are not considered to be virulent mechanisms but are essential for the life of the bacterium. Mutants of Salmonella Gallinarum containing defective genes, related to cobalamin biosynthesis and which Salmonella spp. has to be produced to survive when it is in an anaerobic environment, were produced in this study. Salmonella Gallinarum is an intracellular parasite. Therefore, this study could provide information about whether vitamin B12 biosynthesis might be essential to its survival in the host. The results showed that the singular deletion in cbiA or cobS genes did not interfere in the life of Salmonella Gallinarum in the host, perhaps because single deletion is not enough to impede vitamin B12 biosynthesis. It was noticed that diluted SG mutants with single deletion produced higher mortality than the wild strain of SG. When double mutation was carried out, the Salmonella Gallinarum mutant was unable to provoke mortality in susceptible chickens. This work showed that B12 biosynthesis is a very important step in the metabolism of Salmonella Gallinarum during the infection of the chickens. Further research on bacterium physiology should be carried out to elucidate the events described in this research and to assess the mutant as a vaccine strain.
authors.affiliation: Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista , Jaboticabal, SP , Brasil.
authors.forename: Jacqueline Boldrin
authors.initials: JB
authors.lastname: de Paiva
authors.forename: Rafael Antonio Casarin
authors.initials: RA
authors.lastname: Penha Filho
authors.forename: Yuli Melisa Sierra
authors.initials: YM
authors.lastname: Arguello
authors.forename: Angelo
authors.initials: A
authors.lastname: Berchieri Junior
authors.forename: Manuel Victor Franco
authors.initials: MV
authors.lastname: Lemos
authors.forename: Paul A
authors.initials: PA
authors.lastname: Barrow
keywords: Salmonella Gallinarum; attenuated strain; cbiA and cobS genes; cobalamin biosynthesis
references.citation: J Bacteriol. 1997 Feb;179(4):1013-22
references.pmid: 9023178
references.citation: Avian Dis. 1981 Jan-Mar;25(1):38-52
references.pmid: 7271663
references.citation: J Bacteriol. 2007 Jul;189(13):4555-60
references.pmid: 17483216
references.citation: J Bacteriol. 1993 Jun;175(11):3303-16
references.pmid: 8501034
references.citation: J Hyg (Lond). 1980 Jun;84(3):479-88
references.pmid: 7052226
references.citation: Infect Immun. 1992 Sep;60(9):3518-22
references.pmid: 1500158
references.citation: Mol Microbiol. 2003 Jun;48(6):1633-45
references.pmid: 12791144
references.citation: J Bacteriol. 1992 Apr;174(7):2253-66
references.pmid: 1312999
references.citation: J Med Microbiol. 1992 Jun;36(6):389-97
references.pmid: 1613777
references.citation: J Comp Pathol. 1955 Jan;65(1):37-54
references.pmid: 13233365
references.citation: Mol Microbiol. 1994 Aug;13(3):541-53
references.pmid: 7527895
references.citation: Microbiology (Reading). 2004 May;150(Pt 5):1385-1395
references.pmid: 15133100
references.citation: J Bacteriol. 1988 Sep;170(9):3855-63
references.pmid: 3045078
references.citation: Epidemiol Infect. 2005 Dec;133(6):959-78
references.pmid: 16274493
references.citation: J Bacteriol. 1989 Dec;171(12):6726-33
references.pmid: 2687248
references.citation: Science. 1993 Jan 29;259(5095):686-8
references.pmid: 8430319
references.citation: Nature. 1975 Mar 13;254(5496):150-1
references.pmid: 1090845
references.citation: FEMS Microbiol Lett. 1996 Jun 1;139(2-3):121-6
references.pmid: 8674979
references.citation: J Bacteriol. 1995 Nov;177(22):6371-80
references.pmid: 7592411
references.citation: Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):11798-803
references.pmid: 10518530
references.citation: J Biol Chem. 1982 Jun 10;257(11):6544-50
references.pmid: 6804468
references.citation: Microbiology (Reading). 2000 Mar;146 ( Pt 3):551-571
references.pmid: 10746759
references.citation: Avian Pathol. 1991 Jun;20(2):335-48
references.pmid: 18680027
references.citation: Avian Pathol. 2000 Oct;29(5):423-31
references.pmid: 19184834
references.citation: Vaccine. 1999 Jun 4;17(20-21):2538-45
references.pmid: 10418900
references.citation: J Bacteriol. 1992 Apr;174(7):2267-72
references.pmid: 1313000
references.citation: Nat Prod Rep. 2002 Aug;19(4):390-412
references.pmid: 12195810
references.citation: Mol Microbiol. 2002 Dec;46(5):1451-64
references.pmid: 12453229
references.citation: J Bacteriol. 1999 Nov;181(21):6573-84
references.pmid: 10542156
references.citation: Vaccine. 1998 May-Jun;16(9-10):899-903
references.pmid: 9682335
references.citation: J Bacteriol. 1987 May;169(5):2251-8
references.pmid: 3032913
references.citation: Infect Immun. 2003 Jun;71(6):3392-401
references.pmid: 12761123
references.citation: Infect Immun. 1990 Jul;58(7):2283-8
references.pmid: 2194968
references.citation: Vaccine. 1993;11(4):457-62
references.pmid: 8470431
references.citation: J Bacteriol. 1988 May;170(5):2078-82
references.pmid: 3129401
references.citation: J Bacteriol. 2008 Feb;190(4):1160-71
references.pmid: 17981976
references.citation: Mol Gen Genet. 1971;110(4):378-81
references.pmid: 4932889
references.citation: Appl Environ Microbiol. 2007 Dec;73(23):7522-30
references.pmid: 17921269
references.citation: Avian Pathol. 2005 Aug;34(4):362-6
references.pmid: 16147574
references.citation: Epidemiol Infect. 1990 Jun;104(3):413-26
references.pmid: 2189743
references.citation: J Bacteriol. 1992 Jan;174(1):1-7
references.pmid: 1729202
references.citation: J Bacteriol. 2001 Apr;183(8):2463-75
references.pmid: 11274105
references.citation: J Bacteriol. 1989 Dec;171(12):6734-9
references.pmid: 2687249
references.citation: J Bacteriol. 1984 Jul;159(1):206-13
references.pmid: 6376471