title: Antibacterial and antifungal activity of photoactivated ZnO nanoparticles in suspension.
abstract: Antibacterial activity of photoactivated zinc oxide nanoparticles (ZnO NPs) against human pathogens Escherichia coli O157:H7, Listeria monocytogenes ATCL3C 7644 and plant pathogen Botrytis cinerea was investigated. Data indicate that photoactivated (λ = 400 nm) ZnO NPs at concentration 1 × 10(-3)M and incubation time 60 min reduced population of both bacteria by 7 log (CFU/ml). Clear dependence of antimicrobial properties of ZnO NPs on used concentration and incubation time was found. Scanning electron microscopy (SEM) images of treated bacteria indicate that treatment induced cell wall disintegration and lysis. Results obtained on examination of antifungal activity of ZnO NPs reveal that significant photoinactivation (58%) of B. cinerea was observed at NPs concentration 5 × 10(-3)M and incubation time of 24h. SEM analysis confirmed that substantial morphological changes occur in the microfungus after treatment. The data suggest that ZnO NPs in the presence of visible light exhibit strong antibacterial and antifungal activity. Such ZnO NPs properties obviously could be used for the development of effective fungicides in agriculture or innovative physical antibacterial agents, so important in medicine and food microbial control.
authors.affiliation: Vilnius University, Institute of Applied Research, Sauletekio 10, 10223 Vilnius, Lithuania.
authors.forename: Kristina
authors.initials: K
authors.lastname: Kairyte
authors.forename: Arunas
authors.initials: A
authors.lastname: Kadys
authors.forename: Zivile
authors.initials: Z
authors.lastname: Luksiene
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry* / Q000494:pharmacology; D020171:Botrytis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D008027:Light; D008089:Listeria monocytogenes / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000528:radiation effects; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D015034:Zinc Oxide
keywords: Antimicrobial activity; Botrytis; Escherichia; Listeria; Photoinactivation; Zinc oxide nanoparticles