title: The effect of pstS and phoB on quorum sensing and swarming motility in Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa is an opportunistic pathogen that can cause a wide range of infections and inflammations in a variety of hosts, such as chronic biofilm associated lung infections in Cystic Fibrosis patients. Phosphate, an essential nutrient, has been recognized as an important signal that affects virulence in P. aeruginosa. In the current study we examined the connection between phosphate regulation and surface motility in P. aeruginosa. We focused on two important genes, pstS, which is involved in phosphate uptake, and phoB, a central regulator that responds to phosphate starvation. We found that a mutant lacking pstS is constantly starved for phosphate and has a hyper swarming phenotype. Phosphate starvation also induced swarming in the wild type. The phoB mutant, on the other hand, did not express phosphate starvation even when phosphate was limited and showed no swarming. A double mutant lacking both genes (pstS and phoB) showed a similar phenotype to the phoB mutant (i.e. no swarming). This highlights the role of phoB in controlling swarming motility under phosphate-depleted conditions. Finally, we were able to demonstrate that PhoB controls swarming by up-regulating the Rhl quorum sensing system in P. aeruginosa, which resulted in hyper production of rhamonlipids: biosurfactants that are known to induce swarming motility.
authors.affiliation: The Institute for Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan, Israel ; The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.
authors.forename: Inna
authors.initials: I
authors.lastname: Blus-Kadosh
authors.forename: Anat
authors.initials: A
authors.lastname: Zilka
authors.forename: Gal
authors.initials: G
authors.lastname: Yerushalmi
authors.forename: Ehud
authors.initials: E
authors.lastname: Banin
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D006017:Glycolipids / Q000096:biosynthesis; D009068:Movement; D009154:Mutation; D010710:Phosphates / Q000172:deficiency / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000166:cytology* / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D053038:Quorum Sensing
chemical_list: D001426:Bacterial Proteins; D006017:Glycolipids; D010710:Phosphates; C418382:rhamnolipid; C061278:PhoB protein, Bacteria
references.citation: Curr Opin Microbiol. 2010 Apr;13(2):198-203
references.pmid: 20171928
references.citation: Clin Microbiol Rev. 2006 Apr;19(2):403-34
references.pmid: 16614255
references.citation: BMC Microbiol. 2005 May 23;5:30
references.pmid: 15907219
references.citation: Mol Microbiol. 2005 Dec;58(5):1430-40
references.pmid: 16313627
references.citation: Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11229-34
references.pmid: 10500159
references.citation: Appl Microbiol Biotechnol. 2005 Oct;68(6):718-25
references.pmid: 16160828
references.citation: Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):197-201
references.pmid: 8278364
references.citation: Surgery. 2008 Aug;144(2):189-97
references.pmid: 18656625
references.citation: Gene. 1991 Jan 2;97(1):109-21
references.pmid: 1899844
references.citation: Appl Environ Microbiol. 2012 Sep;78(18):6762-8
references.pmid: 22773629
references.citation: Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1490-4
references.pmid: 7878006
references.citation: J Bacteriol. 2006 Dec;188(24):8601-6
references.pmid: 17028277
references.citation: Genome Res. 2005 Apr;15(4):583-9
references.pmid: 15805499
references.citation: Appl Environ Microbiol. 2012 Aug;78(15):5060-9
references.pmid: 22582064
references.citation: Trends Microbiol. 2008 Oct;16(10):496-506
references.pmid: 18775660
references.citation: FEMS Microbiol Rev. 2004 Jun;28(3):261-89
references.pmid: 15449604
references.citation: Microbiology (Reading). 2003 Aug;149(Pt 8):2005-2013
references.pmid: 12904540
references.citation: Environ Microbiol. 2009 Feb;11(2):279-88
references.pmid: 19196266
references.citation: J Basic Microbiol. 2003;43(1):36-46
references.pmid: 12596240
references.citation: PLoS Pathog. 2008 Feb 8;4(2):e43
references.pmid: 18282104
references.citation: J Bacteriol. 1997 Sep;179(18):5756-67
references.pmid: 9294432
references.citation: J Infect Public Health. 2009;2(3):101-11
references.pmid: 20701869
references.citation: Nucleic Acids Res. 1989 May 11;17(9):3469-78
references.pmid: 2657660
references.citation: J Bacteriol. 1977 Aug;131(2):505-11
references.pmid: 328484
references.citation: Nature. 2000 Aug 31;406(6799):959-64
references.pmid: 10984043
references.citation: Microbiology (Reading). 2010 Aug;156(Pt 8):2271-2282
references.pmid: 20488878
references.citation: Prog Retin Eye Res. 2004 Jan;23(1):1-30
references.pmid: 14766315
references.citation: J Bacteriol. 2000 Nov;182(21):5990-6
references.pmid: 11029417
references.citation: FEMS Microbiol Rev. 2006 Mar;30(2):274-91
references.pmid: 16472307
references.citation: FEMS Microbiol Rev. 2008 May;32(3):461-73
references.pmid: 18248418
references.citation: Microbiol Rev. 1979 Mar;43(1):73-102
references.pmid: 111024
references.citation: J Bacteriol. 2010 Jun;192(12):2973-80
references.pmid: 20154129
references.citation: Infect Immun. 2007 Nov;75(11):5313-24
references.pmid: 17724070
references.citation: Annu Rev Microbiol. 2003;57:677-701
references.pmid: 14527295
references.citation: Gene. 1995 May 26;158(1):15-22
references.pmid: 7789804
references.citation: J Bacteriol. 2005 Nov;187(21):7351-61
references.pmid: 16237018
references.citation: Microbiology (Reading). 2007 Oct;153(Pt 10):3264-3274
references.pmid: 17906126
references.citation: J Bacteriol. 1966 Oct;92(4):851-5
references.pmid: 4959048