title: Characterisation of the transcriptomes of genetically diverse Listeria monocytogenes exposed to hyperosmotic and low temperature conditions reveal global stress-adaptation mechanisms.
abstract: The ability of Listeria monocytogenes to adapt to various food and food- processing environments has been attributed to its robustness, persistence and prevalence in the food supply chain. To improve the present understanding of molecular mechanisms involved in hyperosmotic and low-temperature stress adaptation of L. monocytogenes, we undertook transcriptomics analysis on three strains adapted to sub-lethal levels of these stress stimuli and assessed functional gene response. Adaptation to hyperosmotic and cold-temperature stress has revealed many parallels in terms of gene expression profiles in strains possessing different levels of stress tolerance. Gene sets associated with ribosomes and translation, transcription, cell division as well as fatty acid biosynthesis and peptide transport showed activation in cells adapted to either cold or hyperosmotic stress. Repression of genes associated with carbohydrate metabolism and transport as well as flagella was evident in stressed cells, likely linked to activation of CodY regulon and consequential cellular energy conservation.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, California, United States of America ; Food Safety Centre, Tasmanian Institute of Agriculture, University of Tasmania, Hobart, Tasmania, Australia.
authors.forename: Juliana
authors.initials: J
authors.lastname: Durack
authors.forename: Tom
authors.initials: T
authors.lastname: Ross
authors.forename: John P
authors.initials: JP
authors.lastname: Bowman
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D050260:Carbohydrate Metabolism / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D002462:Cell Membrane / Q000378:metabolism; D003080:Cold Temperature; D020869:Gene Expression Profiling; D014644:Genetic Variation; D008089:Listeria monocytogenes / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D009997:Osmotic Pressure; D013045:Species Specificity; D013312:Stress, Physiological / Q000235:genetics*; D014158:Transcription, Genetic
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