title: Adaptive and cross-protective responses against cadmium and zinc toxicity in cadmium-resistant bacterium isolated from a zinc mine.
abstract: Cadmium (Cd) is a major environmental hazard, which usually is detected in its ionic form of Cd(2+). It also causes adverse toxic effects on human health and other living organisms. Cd-resistant bacteria were isolated from Cd-contaminated soils. One isolate, TAK1, was highly resistance level to Cd toxicity. TAK1 was isolated from soil contaminated with a high Cd concentration (204.1 mg.kg(-1)). The result of 16S rDNA sequence analysis found that the TAK1 showed the similarity to Ralstonia sp. Physiological adaptive and cross-protective responses to Cd and Zn killing were investigated in Ralstonia sp.TAK1. Exposure to a low concentration of Cd induced adaptive resistance to higher concentrations of Cd. In addition, pretreatment of Ralstonia sp.TAK1 with an inducing concentration of Cd conferred cross-protective response against subsequent exposure to the lethal concentrations of Zn. The induced adaptive and cross-protective response Ralstonia sp.TAK1 required newly synthesized protein(s). Cd-induced adaptive and cross-protective responses against Cd and Zn toxicity are the important mechanisms used by Ralstonia sp.TAK1 to survive in the heavy metal-contaminated environments. These findings might lead to the use of Ralstonia sp.TAK1 for microbial based remediation in Cd and Zn-contaminated soils.
authors.affiliation: Laboratory of Environmental Biotechnology, Faculty of Environment and Resource Studies, Mahidol University , Salaya, Nakhonpathom 73170 , Thailand.
authors.forename: Benjaphorn
authors.initials: B
authors.lastname: Prapagdee
authors.forename: Anchulee
authors.initials: A
authors.lastname: Watcharamusik
keywords: Bacterial responses; Cadmium; Metal Resistance; Ralstonia sp.; Zinc
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