title: Reproductive isolation revealed in preliminary crossbreeding experiments using field collected Triatoma dimidiata (Hemiptera: Reduviidae) from three ITS-2 defined groups.
abstract: Triatoma dimidiata, a Chagas disease vector distributed in Mexico, Central America, Colombia, Venezuela, Peru and Ecuador, has been studied using genetic markers and four groups have been defined by ITS-2 sequences: 1A, 1B, 2 and 3. To gather evidence on the divergence and reproductive isolation among T. dimidiata ITS-2 groups, we carried out 15 crossbreeding experiments with field-collected sylvan and domestic T. dimidiata from Guatemala where three groups are found: 1A, 2 and 3. Reciprocal crosses between individuals from groups 1A and 2, and a cross between group 2 individuals from different habitats, produced an average 129.78±42.29 eggs with hatching success ranging from 31.6 to 90.1%. The offspring of these crosses reached the adult stage, and crosses between F1 insects produced eggs. These results suggest that there are no pre- or post-zygotic reproductive barriers between groups 1A and 2, or within group 2. Crosses between group 3 females and males from groups 1A or 2 produced on average 85.67±30.26 eggs and none of them hatched. These results support the existence of pre-zygotic barriers between T. dimidiata group 3 and groups 1A and 2. The group 3 individuals were collected in sylvatic environments in Yaxha, Peten, Guatemala. Previously, distinct chromosomal characteristics (cytotype 3) were described in individuals from this population. Based on this evidence we suggest that this population is divergent at the species level from other T. dimidiata populations.
authors.affiliation: Laboratorio de Entomología Aplicada y Parasitología, Escuela de Biología, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala, Edificio T-10 2do nivel, Ciudad Universitaria Zona 12, Ciudad de Guatemala, Guatemala. Electronic address: mahuasp@gmail.com.
authors.forename: Mauricio
authors.initials: M
authors.lastname: García
authors.forename: Marianela
authors.initials: M
authors.lastname: Menes
authors.forename: Patricia L
authors.initials: PL
authors.lastname: Dorn
authors.forename: Carlota
authors.initials: C
authors.lastname: Monroy
authors.forename: Bethany
authors.initials: B
authors.lastname: Richards
authors.forename: Francisco
authors.initials: F
authors.lastname: Panzera
authors.forename: Dulce María
authors.initials: DM
authors.lastname: Bustamante
mesh_terms: D000818:Animals; D002489:Central America; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005260:Female; D005838:Genotype; D006824:Hybridization, Genetic; D007303:Insect Vectors; D008297:Male; D060047:Reproductive Isolation; D014224:Triatoma / Q000145:classification / Q000235:genetics / Q000502:physiology*
chemical_list: D021903:DNA, Ribosomal Spacer
keywords: Chagas disease; Crossbreeding; ITS-2; Reproductive isolation; Triatoma dimidiata
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