title: Production of inulinase from Kluyveromyces marxianus using dahlia tuber extract.
abstract: Various carbon sources were evaluated for production of inulinase by yeast, Kluyveromyces marxianus MTCC 3995. Highest inulinase activity was observed with Dahlia extract (25.3 nkat mL(-1)) as carbon source. The enzyme activity was 1.4 folds higher than that observed in media containing pure chicory inulin (17.8 nkat mL(-1)). The yeast showed good growth on a simple medium containing dahlia extract (20% w/v) and yeast extract (2%w/v) as carbon and nitrogen source respectively, in 96 h. at 28°C and 120 rpm. Lowest inulinase yield (4.8 nkat mL(-1)) was seen in the medium containing glucose as C-source. Although varied inulinase levels were noticed on different C- sources, Inulinase: Sucrase (I/S) ratios were noticed to be similar. Among various protein sources tested, yeast extract was found to be the best source followed by beef extract (17.9 nkat mL(-1)) and peptone (13.8 nkat mL(-1)). The enzyme was optimally active at pH (4.0) and 50°C. TLC analysis of end product revealed that inulinase hydrolyzed inulin exclusively into fructose. Results suggest that the dahlia extract induced exoinulinase synthesis in Kluyveromyces marxianus and can be utilized as a potential substrate for inulinase production.
authors.affiliation: Department of Applied Microbiology and Biotechnology, Dr. Hari Singh Gour University , Sagar, M.P. 470003 , India.
authors.forename: Sumat Chand
authors.initials: SC
authors.lastname: Jain
authors.forename: P C
authors.initials: PC
authors.lastname: Jain
authors.forename: Naveen
authors.initials: N
authors.lastname: Kango
keywords: Inulinase; Kluyveromyces marxianus; dahlia; fructose
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