title: Kobophenol A enhances proliferation of human osteoblast-like cells with activation of the p38 pathway.
abstract: Bone cell proliferation, bone formation, and bone resorption are the main factors involved in the homeostasis of the bone mass. Osteoblast death is a problem experienced by postmenopause women. Herbal medicines have attracted considerable attention for use as a drug or a drug substitute in the treatment of bone-related diseases, such as osteoporosis. This study investigated the effects of kobophenol A on the proliferation in human osteoblast cells. Kobophenol A stimulated the proliferation of osteoblast cells by the increases in DNA synthesis and the enhancement of cell cycle progression. Kobophenol A stimulation induced the expression of the cyclin B1 and cyclin-dependent kinase 1 (CDK1). Treatment of osteoblast cells with p38 MAPK inhibitor SB203580 significantly inhibited kobophenol A-enhanced proliferation. In addition, kobophenol A induced phosphorylation of p38 MAPK. Treatment of osteoblast cells with kobophenol A resulted in improvement of ROS scavenging activity. Moreover, kobophenol A treatment up-regulated the Bcl-2 level, but down-regulated the level of Bax expression. We also demonstrate that kobophenol A increased alkaline phosphatase (ALP) activity after 2 days. Taken together, the results of this study reveal that kobophenol A has proliferative effects and enhances ALP activity in osteoblast cells and these findings provide insights into the development of a therapeutic approach of kobophenol A in the prevention of osteoporosis and other bone disorders.
authors.affiliation: Division of Immunopharmacology, School of Pharmacy, Sungkyunkwan University, Suwon, Kyunggi-do, 440-746, Republic of Korea.
authors.forename: Jong-Hwan
authors.initials: JH
authors.lastname: Kwak
authors.forename: Sung-Ryul
authors.initials: SR
authors.lastname: Lee
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Park
authors.forename: Hye-Eun
authors.initials: HE
authors.lastname: Byun
authors.forename: Eun-Hwa
authors.initials: EH
authors.lastname: Sohn
authors.forename: Byung-Oh
authors.initials: BO
authors.lastname: Kim
authors.forename: Dong-Kwon
authors.initials: DK
authors.lastname: Rhee
authors.forename: Suhkneung
authors.initials: S
authors.lastname: Pyo
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D050071:Bone Density Conservation Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D009569:Nitric Oxide / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013267:Stilbenes / Q000494:pharmacology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D050071:Bone Density Conservation Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D013267:Stilbenes; C510380:kobophenol A; D009569:Nitric Oxide; D048051:p38 Mitogen-Activated Protein Kinases; D000469:Alkaline Phosphatase
keywords: Kobophenol A; Osteoblast; Proliferation; ROS; p38 MAPK