title: HPLC analysis of carotenoids from tomatoes using cross-linked C18 column and MS detection.
abstract: This study was conducted to analyze carotenoids from tomatoes by high-performance liquid chromatography using reversed-phase C18 silica having cross-linked end-capping with diode array and mass spectrometric detection. An efficient gradient elution system was developed to achieve good and reliable separation of both major and minor carotenoids as well as their isomers. Resolution of lycopene, β-carotene and their isomers was 0.91-3.97 and 1.02-2.86 with cross-linked and conventional C18 column, respectively. The % recovery for zeaxanthin, lycopene and β-carotene was found to be in the range of 89-97%. Limits of detection and quantification of 19.44 and 64.79 ng/mL for zeaxanthin, 15.6 and 52.4 ng/mL for lycopene and 8.28 and 27.61 ng/mL for β-carotene were determined. More carotenoid compounds could be separated and detected with the new method as compared with conventional C18 column. Hyphenation of HPLC with photodiode array and mass spectrometry detectors assisted in detection of tetra-dehydrocarotenoid and fatty acid diesters of xanthophylls in tomato products. Content of all-trans-lycopene, β-carotene and total carotenoid in different industrial tomatoes tested was found to range between 41.87 and 84.65, 0.89 and 1.50 and 53.22 and 112.60 µg/g fresh weight, respectively.
authors.affiliation: Regional University Science Centre, Szent István University, Páter K. u. 1, Gödöllő H-2103, Hungary hdaood682@gmail.com.
authors.forename: Hussein G
authors.initials: HG
authors.lastname: Daood
authors.affiliation: Vichem Ltd., Herman Ottó u. 15, Budapest H-1022, Hungary.
authors.forename: Gyula
authors.initials: G
authors.lastname: Bencze
authors.affiliation: Univer Product Co., Szolnoki u. 35, Kecskemét H-6000, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Palotás
authors.affiliation: Regional University Science Centre, Szent István University, Páter K. u. 1, Gödöllő H-2103, Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Pék
authors.affiliation: Uzbe Research Institute of Cotton Breeding and Seed Production, University Street N1, Ribray District, Tashkent 218112, Uzbekistan.
authors.forename: Akmal
authors.initials: A
authors.lastname: Sidikov
authors.affiliation: Regional University Science Centre, Szent István University, Páter K. u. 1, Gödöllő H-2103, Hungary.
authors.forename: Lajos
authors.initials: L
authors.lastname: Helyes
mesh_terms: D002338:Carotenoids / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D007536:Isomerism; D057230:Limit of Detection; D000077276:Lycopene; D018551:Solanum lycopersicum / Q000737:chemistry*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D015203:Reproducibility of Results; D065146:Zeaxanthins / Q000032:analysis / Q000737:chemistry; D019207:beta Carotene / Q000032:analysis / Q000737:chemistry
chemical_list: D065146:Zeaxanthins; D019207:beta Carotene; D002338:Carotenoids; D000077276:Lycopene