title: Association of interferon-gamma induced protein 10 promoter polymorphisms with the disease progression of hepatitis B virus infection in Chinese Han population.
abstract: OBJECTIVE
  Interferon-gamma induced protein 10 (IP-10) was suggested to be involved in liver injury in viral hepatitis. This study aimed to investigate the impact of the single nucleotide polymorphisms (SNP) G-201A (rs1439490) in IP-10 gene on disease progression of hepatitis B virus (HBV) infection.
  
  
  METHODS
  The -201 SNP in IP-10 promoter was genotyped from 577 patients with different illness categories and 275 health controls; In vitro IP-10 promoter activity was compared between haplotype GG and AA homozygotes using luciferase reporter system in HepG2 cells. In vivo expression of IP-10 was compared between patients with -201 AA genotype and GG genotype.
  
  
  RESULTS
  The detected frequency of G-201A SNP was 17.8%, 25.3%, 26.6%, and 13.8% for patients with acute hepatitis B (AHB), patients with mild chronic hepatitis B (CHB-M), patients with severe chronic hepatitis B (CHB-S), and health controls, respectively. In vitro IP-10 promoter-driven luciferase activity in pGL3-Enhancer-201A transfected HepG2 cells was 1.43-fold higher than that in pGL3-Enhancer-201G transfected HepG2 cells (P<0.01). In vivo IP-10 transcriptional expression of peripheral blood mononuclear cells was 1.38-fold higher in patients with -201 AA genotype than in patients with -201 GG genotype (P<0.01).
  
  
  CONCLUSIONS
  G-201A in promoter region of IP-10 gene was associated with liver disease progression in patients with HBV infection through up-regulating IP-10 expression.
authors.affiliation: Viral Hepatitis Research Laboratory, Beijing 302 Hospital, Beijing, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Liming
authors.initials: L
authors.lastname: Liu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Li
authors.forename: Siyu
authors.initials: S
authors.lastname: Bai
authors.forename: Yihui
authors.initials: Y
authors.lastname: Rong
authors.forename: Haibin
authors.initials: H
authors.lastname: Wang
authors.forename: Yuanli
authors.initials: Y
authors.lastname: Mao
authors.forename: Shaojie
authors.initials: S
authors.lastname: Xin
authors.forename: Dongping
authors.initials: D
authors.lastname: Xu
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics; D054357:Chemokine CXCL10 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006509:Hepatitis B / Q000235:genetics*; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic / Q000235:genetics*; D055815:Young Adult
chemical_list: C114753:CXCL10 protein, human; D054357:Chemokine CXCL10
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