title: Use of pcr-rflp of the fla a gene for detection and subtyping of Campylobacter jejuni strains Potentially related to Guillain-barré syndrome, isolated from humans and animals.
abstract: The objectives of the present study were the subtyping of Campylobacter jejuni subsp. jejuni strains obtained from humans and different animal species using PCR-RFLP, and the detection, by means of the same technique, of strains related to serotype PEN O19:LIO 7, the main C. jejuni serotype linked to Guillain-Barré Syndrome (GBS). Seventy C. jejuni strains isolated from human feces (n=33), primates (n=15), dogs (n=5), swine (n=2), bovines (n=1), abortion material from goats (n=2) and poultry carcasses (n=12), all collected in the state of São Paulo, were subtyped by means of PCR-RFLP of fla A gene, using restriction endonucleases Hae III, Afa I and Mbo I. Seven subtypes were observed when using the enzyme Hae III; eight when using Mbo I; and seven when using Afa I. The combination of the three endonucleases led to 16 fla-RFLP subtypes, from which ten subtypes shared strains of human and animal origin. From these, seven subtypes were observed in human and broiler strains. In eight subtypes, the other animal species shared patterns with human strains. It was inferred that, besides broilers, swine, goats, dogs and primates may be sources of infection for human in São Paulo. PCR-RFLP is a highly discriminatory technique that may be applied to molecular epidemiology studies of samples from different origins. Besides, the study also enabled the detection of two human strains and two primate strains related to serotype PEN O19: LIO 7.
authors.affiliation: Centro de Pesquisa e Desenvolvimento de Sanidade Animal, Instituto Biológico , São Paulo, SP , Brasil.
authors.forename: E
authors.initials: E
authors.lastname: Scarcelli
authors.forename: R M
authors.initials: RM
authors.lastname: Piatti
authors.forename: R
authors.initials: R
authors.lastname: Harakava
authors.forename: S
authors.initials: S
authors.lastname: Miyashiro
authors.forename: F R
authors.initials: FR
authors.lastname: Campos
authors.forename: M C A
authors.initials: MC
authors.lastname: Souza
authors.forename: M V
authors.initials: MV
authors.lastname: Cardoso
authors.forename: S R
authors.initials: SR
authors.lastname: Teixeira
authors.forename: M E
authors.initials: ME
authors.lastname: Genovez
keywords: Campylobacter jejuni; Guillain-Barré Syndrome; PCR-RFLP; Paenibacillus sp.; subtyping
references.citation: FEMS Immunol Med Microbiol. 2005 Aug 1;45(2):311-9
references.pmid: 15990285
references.citation: FEMS Microbiol Lett. 1996 Sep 1;142(2-3):133-8
references.pmid: 8810495
references.citation: Lett Appl Microbiol. 2002;34(5):354-8
references.pmid: 11967058
references.citation: Vet Clin North Am Food Anim Pract. 1998 Mar;14(1):31-40
references.pmid: 9532665
references.citation: J Appl Microbiol. 2001 Feb;90(2):285-93
references.pmid: 11168732
references.citation: Emerg Infect Dis. 1998 Apr-Jun;4(2):263-8
references.pmid: 9621196
references.citation: Zoonoses Public Health. 2007;54(1):2-7
references.pmid: 17359440
references.citation: Rev Inst Med Trop Sao Paulo. 2006 Nov-Dec;48(6):307-10
references.pmid: 17221125
references.citation: J Clin Microbiol. 2007 Jan;45(1):102-8
references.pmid: 17093018
references.citation: J Infect Dis. 1997 Dec;176 Suppl 2:S125-8
references.pmid: 9396695
references.citation: J Infect Dis. 1997 Dec;176 Suppl 2:S129-34
references.pmid: 9396696
references.citation: J Clin Microbiol. 1982 May;15(5):761-8
references.pmid: 7096555
references.citation: Acta Neurol Scand. 2003 Aug;108(2):102-8
references.pmid: 12859286
references.citation: J Clin Microbiol. 2001 Nov;39(11):3823-9
references.pmid: 11682493
references.citation: Appl Environ Microbiol. 2000 Sep;66(9):3917-23
references.pmid: 10966409
references.citation: J Clin Microbiol. 1997 Nov;35(11):2810-4
references.pmid: 9350739
references.citation: Enferm Infecc Microbiol Clin. 2006 Aug-Sep;24(7):437-9
references.pmid: 16956532
references.citation: Emerg Infect Dis. 2002 Sep;8(9):937-42
references.pmid: 12194770
references.citation: J Clin Microbiol. 2000 Jun;38(6):2297-301
references.pmid: 10834992
references.citation: J Clin Microbiol. 2001 Jul;39(7):2386-90
references.pmid: 11427543
references.citation: J Clin Microbiol. 2001 Apr;39(4):1353-9
references.pmid: 11283056
references.citation: Rev Argent Microbiol. 2005 Apr-Jun;37(2):81-3
references.pmid: 16178461
references.citation: Int J Food Microbiol. 2006 Jul 1;110(1):24-33
references.pmid: 16712997
references.citation: J Infect Dis. 1992 Jan;165(1):183
references.pmid: 1727892
references.citation: J Vet Med Sci. 2006 Aug;68(8):815-9
references.pmid: 16953081