title: ROCK mediates the inflammatory response in thrombin induced microglia.
abstract: To investigate whether the ROCK pathway is involved in thrombin-induced microglial inflammatory response, thrombin-induced microglia were pretreated with the thrombin inhibitor argatroban or a ROCK inhibitor Y-27632. Microglial inflammatory response was evaluated by phagocytosis of fluorescein labeled latex beads analyses and inflammatory mediators' expression such as nitric oxide (NO) and tumor necrosis factor-alpha (TNF-а). Compared to non-induced microglia, thrombin-induced microglia show significantly enhanced phagocytotic capacity and increased ROCK, NO and TNF-а expression. Pretreatment of thrombin-induced microglia with argatroban or Y-27632 significantly decreased phagocytotic capacity and reduced ROCK, NO and TNF-α expression. Therefore, the ROCK pathway may play a vital role in the mechanisms by which thrombin induces microglia in the inflammatory response.
authors.affiliation: Department of Neurology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu Province, China.
authors.forename: Guiyun
authors.initials: G
authors.lastname: Cui
authors.forename: Tao
authors.initials: T
authors.lastname: Zuo
authors.forename: Qiuchen
authors.initials: Q
authors.lastname: Zhao
authors.forename: Jinxia
authors.initials: J
authors.lastname: Hu
authors.forename: Peisheng
authors.initials: P
authors.lastname: Jin
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Jia
authors.initials: J
authors.lastname: Jing
authors.forename: Jienan
authors.initials: J
authors.lastname: Zhu
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Hua
authors.forename: Xinchun
authors.initials: X
authors.lastname: Ye
mesh_terms: D000577:Amides / Q000494:pharmacology; D000818:Animals; D000991:Antithrombins / Q000494:pharmacology; D001120:Arginine / Q000031:analogs & derivatives; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D019793:Fluorescein; D005456:Fluorescent Dyes; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D017628:Microglia / Q000276:immunology / Q000378:metabolism*; D008863:Microspheres; D009569:Nitric Oxide / Q000378:metabolism; D010587:Phagocytosis; D010875:Pipecolic Acids / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013449:Sulfonamides; D013917:Thrombin / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D000577:Amides; D000991:Antithrombins; D005456:Fluorescent Dyes; D010875:Pipecolic Acids; D011725:Pyridines; D013449:Sulfonamides; D014409:Tumor Necrosis Factor-alpha; C108830:Y 27632; D009569:Nitric Oxide; D001120:Arginine; D054460:rho-Associated Kinases; D013917:Thrombin; C031942:argatroban; D019793:Fluorescein
keywords: Argatroban; Inflammation; Microglia; ROCK; Y-27632