title: Rapid diagnosis of aneuploidy in chromosomes 13, 18, 21, X and Y by quantitative fluorescence-PCR combined with short tandem repeat and fluorescence-labeled homologous gene quantitative‑PCR using 4-color fluorescently labeled universal primers.
abstract: The present study aimed to develop a rapid diagnostic test of aneuploidy in chromosomes 13, 18, 21, X and Y through a program combining short tandem repeat (STR) typing with fluorescence-labeled homologous gene quantitative‑polymerase chain reaction (fHGQ-PCR), which avoids misjudgment risks by using one method alone. Furthermore, fluorescently labeled universal primers not only ensure the accuracy of the results but also reduces the cost of fluorescent labels. The verification of DNA extracted from samples confirmed by karyotype analysis with quantitative fluorescence (QF)-PCR shows that the results obtained using the QF-PCR program are consistent with the results of karyotype analysis in rapidly diagnosing the aneuploidy of chromosomes 13, 18, 21, X and Y.
authors.affiliation: Laboratory of Medical Genetics, Maternal and Child Health Care Hospital of Qinzhou, Guangxi 535099, P.R. China.
authors.forename: Ju
authors.initials: J
authors.lastname: Long
authors.forename: Xuehe
authors.initials: X
authors.lastname: Ye
authors.forename: Xunjin
authors.initials: X
authors.lastname: Weng
authors.forename: Kepeng
authors.initials: K
authors.lastname: Fu
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.forename: Wanrong
authors.initials: W
authors.lastname: Pang
mesh_terms: D000782:Aneuploidy; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics*; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics*; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics*; D041321:Chromosomes, Human, X / Q000235:genetics*; D041322:Chromosomes, Human, Y / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D005453:Fluorescence; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020080:Tandem Repeat Sequences / Q000235:genetics*; D014314:Trisomy / Q000175:diagnosis* / Q000235:genetics
chemical_list: D017931:DNA Primers