title: FimY does not interfere with FimZ-FimW interaction during type 1 fimbria production by Salmonella enterica serovar Typhimurium.
abstract: The production of type 1 fimbriae in Salmonella enterica serovar Typhimurium is controlled, in part, by three proteins, FimZ, FimY, and FimW. Amino acid sequence analysis indicates that FimZ belongs to the family of bacterial response regulators of two-component systems. In these studies, we have demonstrated that introducing a mutation mimicking phosphorylation of FimZ is necessary for activation of its target gene, fimA. In addition, the interaction of FimZ with FimW, a repressor of fimA expression, occurs only when FimZ is phosphorylated. Consequently, the negative regulatory effect of FimW is most likely due to downmodulation of the active FimZ protein. FimY does not appear to function as a response regulator, and its activity can be lost by mimicking the phosphorylation of FimY. Overproduction of FimY cannot alleviate the nonfimbriate phenotype in a FimZ mutant, whereas high levels of FimZ can overcome the nonfimbriate phenotype of a FimY mutant. It appears that FimY acts upstream of FimZ to activate fimA expression.
authors.affiliation: Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Zeiner
authors.forename: Brett E
authors.initials: BE
authors.lastname: Dwyer
authors.forename: Steven
authors.initials: S
authors.lastname: Clegg
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D036781:Fimbriae Proteins / Q000096:biosynthesis / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D010766:Phosphorylation; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism; D020539:Sequence Analysis, Protein
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C419207:FimW protein, Salmonella enterica; C110675:SEF21 protein, Salmonella enteritidis; D036781:Fimbriae Proteins
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