title: The expression of glycosaminoglycans and proteoglycans in the uterine cervix of albino rats after local hyaluronidase infusion.
abstract: OBJECTIVE
  To assess the local effect of hyaluronidase injection on the expression of glycosaminoglycans (GAGs) and proteoglycans (PGs) in the extracellular matrix of the uterine cervix from pregnant albino rats.
  
  
  METHODS
  Ten pregnant rats were divided into two groups on day 18 of pregnancy. The experimental group (Gexp) of rats received an intracervical infusion of 0.02 mL of hyaluronidase diluted to 1 mL with distilled water, whereas the control group (Gc) received 1 mL of distilled water. On day 20 of pregnancy, the pregnant rats were sacrificed and the uterine cervixes from all rats were then dissected. The qualitative expression of hyaluronic acid (HA) was assessed by immunohistochemistry and quantified by sandwich ELISA. To compare the quantitative GAG values between groups, a Student's t-test for independent samples was performed. PGs were also assessed by immunohistochemical analysis.
  
  
  RESULTS
  The electrophoretic profile of newly synthesized radioactively labeled GAGs degraded by specific enzymes showed that there were two predominant GAGs in both Gc and Gexp, i.e. heparan sulfate (HS) and a mixture of hondroitin sulfate (CS) and dermatan sulfate (DS). The concentrations of GAGs showed a significant reduction of CS/DS (p < 0.004) and HS (p < 0.005) relative to Gc. HA staining was less intense in the lamina propria and area surrounding the blood vessels in Gexp compared to Gc. The HA contents were also significantly reduced (p < 0.012).
  
  
  CONCLUSIONS
  Intracervical hyaluronidase infusion promoted a significant reduction in the concentration of sulfated GAGs as assessed by both qualitative (histochemical) and quantitative (fluorometric) measurements of HA.
authors.affiliation: Department of Obstetrics and.
authors.forename: Guilherme Negrão
authors.initials: GN
authors.lastname: Souza
authors.forename: Luiz
authors.initials: L
authors.lastname: Camano
authors.forename: Edward
authors.initials: E
authors.lastname: Araujo Júnior
authors.forename: Helena Bonciani
authors.initials: HB
authors.lastname: Nader
authors.forename: Valquíria
authors.initials: V
authors.lastname: Medeiros
authors.forename: João Roberto Maciel
authors.initials: JR
authors.lastname: Martins
authors.forename: Eduardo
authors.initials: E
authors.lastname: Souza
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002584:Cervix Uteri / Q000187:drug effects* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism*; D006821:Hyaluronoglucosaminidase / Q000008:administration & dosage / Q000494:pharmacology*; D007150:Immunohistochemistry; D007260:Infusion Pumps; D011247:Pregnancy; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006025:Glycosaminoglycans; D011509:Proteoglycans; D006821:Hyaluronoglucosaminidase
keywords: Cervical maturation; extracellular matrix; glycosaminoglycans; hyaluronidase