title: Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA.
abstract: This study aimed to evaluate the use of the FTA elute card(TM) impregnated with cervicovaginal sample directly in the PCR amplification for detection of HPV-DNA. The results were compared to a reference technique. This method was more efficient than the protocol indicated by the manufacturer, identifying 91.7% against 54.2% of the positive samples.
authors.affiliation: Centro de Desenvolvimento Científico e Tecnológico, Fundação Estadual de Produção e Pesquisa em Saúde , Porto Alegre, RS , Brasil ; Universidade Luterana do Brasil , Canoas, RS , Brasil.
authors.forename: Carla R
authors.initials: CR
authors.lastname: Santos
authors.forename: Laura G
authors.initials: LG
authors.lastname: Franciscatto
authors.forename: Regina B
authors.initials: RB
authors.lastname: Barcellos
authors.forename: Sabrina E M
authors.initials: SE
authors.lastname: Almeida
authors.forename: Maria Lucia R
authors.initials: ML
authors.lastname: Rossetti
keywords: FTA elute; HPV; cervical cancer; molecular detection
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