title: Aflp analysis of xanthomonas axonopodis and x. Arboricola strains used in xanthan production studies reveal high levels of polymorphism.
abstract: Amplified fragment length polymorphism (AFLP) was used to analyze the genetic diversity of 14 strains of Xanthomonas arboricola pv. pruni and seven strains of X. axonopodis pv. phaseoli, which are used in xanthan production studies. Relationships identified by the AFLP profiles were assessed for xanthan production capacity, geographical location and host plant. Strains were isolated from 10 different geographic regions in South and Southeast States in Brazil. Data were analyzed for genetic similarity using the Dice coefficient and subjected to UPGMA cluster analysis. A total of 128 AFLP fragments were generated from four primer combinations: EcoRI+C/MseI+0, EcoRI+A/MseI+0, EcoRI+G/MseI+T and EcoRI+G/MseI+A. Of these, 96.1% were polymorphic. X. axonopodis pv. phaseoli (SD = 0.27) was shown to be more polymorphic than X. arboricola pv. pruni (SD = 0.58). All 14 pathovar pruni strains were included in a single main group (SD = 0.58), while the pathovar phaseoli strains were divided into three separate groups, with one group containing five strains (SD = 0.38) and two isolated groups (SD = 0.31 and 0.27) composed of only one strain each. Species were distinguished by three and eight specific AFLP markers present in the pathovar phaseoli and the pathovar pruni, respectively. For the unique strain without xanthan production capacity (X. axonopodis pv. phaseoli str. 48), nine specific AFLP bands were found. There was no evidence that geographic area or host plant influenced genetic heterogeneity. Correlations between AFLP patterns and xanthan production capacity were found in some strains, but were not consistent enough to establish a relationship.
authors.affiliation: Departamento de Ciência e Tecnologia Agroindustrial, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas , Pelotas, RS, , Brasil;
authors.forename: Laurí
authors.initials: L
authors.lastname: Mayer
authors.forename: Wladimir Padilha
authors.initials: WP
authors.lastname: da Silva
authors.forename: Andrea Bittencourt
authors.initials: AB
authors.lastname: Moura
authors.forename: Claire Tondo
authors.initials: CT
authors.lastname: Vendruscolo
keywords: DNA fingerprints;; genomic diversity;; pathovar phaseoli;; pathovar pruni
references.citation: Nucleic Acids Res. 1995 Nov 11;23(21):4407-14
references.pmid: 7501463
references.citation: Syst Appl Microbiol. 2006 Mar;29(2):109-19
references.pmid: 16464692
references.citation: FEMS Microbiol Lett. 2002 Sep 24;215(1):23-31
references.pmid: 12393196
references.citation: Microbiology (Reading). 1999 Jan;145 ( Pt 1):107-114
references.pmid: 10206688
references.citation: Appl Environ Microbiol. 2008 May;74(10):3295-301
references.pmid: 18359831
references.citation: Appl Environ Microbiol. 2007 Mar;73(5):1678-82
references.pmid: 17209062
references.citation: Curr Opin Microbiol. 2006 Oct;9(5):526-31
references.pmid: 16935554
references.citation: Syst Appl Microbiol. 2005 Aug;28(6):494-518
references.pmid: 16104350