title: Developmental expression of translocator protein/peripheral benzodiazepine receptor in reproductive tissues.
abstract: Translocator protein (TSPO) present in the outer mitochondrial membrane has been suggested to be critical for cholesterol import, a rate-limiting step for steroid hormone biosynthesis. Despite the importance of steroidogenesis in regulating reproductive functions, the developmental profile of TSPO expression in the gonads and accessory sex organs has not been completely characterized. As a first step towards understanding the function of TSPO, we studied its expression in male and female murine reproductive organs. We examined testes and ovaries at embryonic days 14.5 and 18.5, and postnatal days 0, 7, 14, 21 and 56 of development. In the adult testis, TSPO was expressed in both Leydig cells and Sertoli cells. In the developing testes TSPO expression was seen in immature Sertoli cells, fetal Leydig cells and gonocytes. In the ovary, TSPO was expressed in the ovarian surface epithelium, interstitial cells granulosa cells and luteal cells. Corpora lutea of ovaries from pregnant mice showed strong expression of TSPO. In the developing ovary, TSPO expression was seen in the squamous pregranulosa cells associated with germ line cysts, together with progressively increasing expression in interstitial cells and the ovarian surface epithelium. In adult mice, the epithelia of other reproductive tissues like the epididymis, prostate, seminal vesicle, oviduct and uterus also showed distinct patterns of TSPO expression. In summary, TSPO expression in both male and female reproductive tissues was not only restricted to steroidogenic cells. Expression in Sertoli cells, ovarian surface epithelium, efferent ductal epithelium, prostatic epithelium, seminal vesiclular epithelium, uterine epithelium and oviductal epithelium suggest either previously unknown sites for de novo steroidogenesis or functions for TSPO distinct from its well-studied role in steroid hormone production.
authors.affiliation: Department of Animal Science, Cornell University, Ithaca, New York, United States of America.
authors.forename: Kanako
authors.initials: K
authors.lastname: Morohaku
authors.forename: Newton S
authors.initials: NS
authors.lastname: Phuong
authors.forename: Vimal
authors.initials: V
authors.lastname: Selvaraj
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian; D004848:Epithelium / Q000254:growth & development / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D012739:Gonadal Steroid Hormones / Q000096:biosynthesis / Q000235:genetics; D006107:Granulosa Cells / Q000166:cytology / Q000378:metabolism*; D007985:Leydig Cells / Q000166:cytology / Q000378:metabolism*; D008184:Luteal Cells / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D018079:Receptors, GABA / Q000235:genetics* / Q000378:metabolism; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism*; D013799:Theca Cells; D050995:Voltage-Dependent Anion Channel 1 / Q000235:genetics / Q000378:metabolism
chemical_list: C463819:Bzrp protein, mouse; D012739:Gonadal Steroid Hormones; D018079:Receptors, GABA; C494763:Vdac1 protein, mouse; D050995:Voltage-Dependent Anion Channel 1
references.citation: Histochemistry. 1989;91(6):495-9
references.pmid: 2767998
references.citation: Endocr Rev. 2001 Apr;22(2):255-88
references.pmid: 11294827
references.citation: J Biochem. 1995 Apr;117(4):875-80
references.pmid: 7592553
references.citation: Curr Pharm Des. 2007;13(23):2385-405
references.pmid: 17692008
references.citation: Endocr Res. 2004 Nov;30(4):677-84
references.pmid: 15666811
references.citation: J Histochem Cytochem. 1999 Jun;47(6):769-76
references.pmid: 10330453
references.citation: Nature. 2004 Mar 11;428(6979):145-50
references.pmid: 15014492
references.citation: Biol Reprod. 1986 Jun;34(5):905-10
references.pmid: 3460641
references.citation: Endocrinology. 1985 Feb;116(2):567-73
references.pmid: 2981667
references.citation: J Exp Biol. 1997 Jan;200(Pt 2):203-16
references.pmid: 9050228
references.citation: Mol Cell Endocrinol. 1995 Apr 28;111(1):R9-12
references.pmid: 7544305
references.citation: Mol Endocrinol. 2005 Feb;19(2):540-54
references.pmid: 15498831
references.citation: Endocrinology. 1977 May;100(5):1341-7
references.pmid: 557406
references.citation: Curr Mol Med. 2012 May;12(4):369-86
references.pmid: 22364126
references.citation: J Androl. 1987 Jul-Aug;8(4):238-46
references.pmid: 3624061
references.citation: Mol Cell Endocrinol. 2011 Apr 10;336(1-2):70-9
references.pmid: 21147195
references.citation: J Bioenerg Biomembr. 2008 Jun;40(3):199-205
references.pmid: 18670869
references.citation: Cells Tissues Organs. 2012;195(5):443-55
references.pmid: 21893932
references.citation: Biol Reprod. 1998 Feb;58(2):470-4
references.pmid: 9475403
references.citation: Mol Pharmacol. 1989 Jan;35(1):157-63
references.pmid: 2464128
references.citation: J Biol Chem. 1989 Dec 5;264(34):20415-21
references.pmid: 2555358
references.citation: Biochim Biophys Acta. 2008 Jun;1778(6):1375-81
references.pmid: 18420025
references.citation: Proc Natl Acad Sci U S A. 2001 Jan 30;98(3):1267-72
references.pmid: 11158628
references.citation: Anat Embryol (Berl). 1994 Oct;190(4):383-8
references.pmid: 7840424
references.citation: Nutr Metab (Lond). 2010 Jun 01;7:47
references.pmid: 20515451
references.citation: Biochem Pharmacol. 1999 Jul 15;58(2):273-8
references.pmid: 10423168
references.citation: Reproduction. 2002 Jun;123(6):743-50
references.pmid: 12052228
references.citation: Nature. 1977 Oct 20;269(5630):702-4
references.pmid: 22814
references.citation: Proc Natl Acad Sci U S A. 1977 Sep;74(9):3805-9
references.pmid: 20632
references.citation: Mol Cell Endocrinol. 2007 Feb;265-266:46-50
references.pmid: 17207924
references.citation: Endocrinology. 1991 Sep;129(3):1481-8
references.pmid: 1651852
references.citation: Endocrinology. 1993 Apr;132(4):1489-96
references.pmid: 8384990
references.citation: Biochim Biophys Acta. 1973 Jan 24;297(1):179-85
references.pmid: 4348322
references.citation: Eur J Pharmacol. 2000 Oct 13;406(2):171-80
references.pmid: 11020479
references.citation: Eur J Pharmacol. 1987 Mar 17;135(2):243-6
references.pmid: 3034629
references.citation: J Biol Chem. 2011 Feb 4;286(5):3460-72
references.pmid: 21106527
references.citation: Biochemistry. 2008 Dec 2;47(48):12886-99
references.pmid: 18975922
references.citation: J Androl. 2011 Nov-Dec;32(6):705-10
references.pmid: 21441422
references.citation: Biol Reprod. 1995 Feb;52(2):217-25
references.pmid: 7711191
references.citation: Dev Biol. 2001 Jun 15;234(2):339-51
references.pmid: 11397004
references.citation: Proc Soc Exp Biol Med. 1992 Nov;201(2):125-40
references.pmid: 1409728
references.citation: Endocrinology. 1978 Jan;102(1):227-35
references.pmid: 570480
references.citation: Proc Natl Acad Sci U S A. 2009 Jul 28;106(30):12542-7
references.pmid: 19620711
references.citation: Anat Rec. 1991 Feb;229(2):177-85
references.pmid: 2012304
references.citation: J Biol Chem. 1997 Dec 19;272(51):32129-35
references.pmid: 9405411
references.citation: Biochim Biophys Acta. 2009 Jul;1791(7):646-58
references.pmid: 19286473
references.citation: Proc Natl Acad Sci U S A. 1987 Feb;84(3):891-5
references.pmid: 3027710
references.citation: J Biol Chem. 1988 Nov 25;263(33):17317-21
references.pmid: 2846560
references.citation: Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7247-51
references.pmid: 8041774
references.citation: J Exp Biol. 2009 Jun;212(Pt 11):1753-61
references.pmid: 19448084
references.citation: Science. 1995 Mar 24;267(5205):1828-31
references.pmid: 7892608
references.citation: Int J Immunopharmacol. 1986;8(7):825-8
references.pmid: 3023246
references.citation: J Biol Chem. 1994 Nov 11;269(45):28314-22
references.pmid: 7961770
references.citation: J Biol Chem. 1986 Jan 15;261(2):576-83
references.pmid: 3001071
references.citation: Pharmacol Biochem Behav. 1986 Jun;24(6):1673-7
references.pmid: 3016760
references.citation: Endocr Res. 2002 Nov;28(4):395-401
references.pmid: 12530641
references.citation: Mol Endocrinol. 1994 May;8(5):654-62
references.pmid: 8058073
references.citation: Mol Cell Endocrinol. 1999 Nov 25;157(1-2):87-94
references.pmid: 10619400
references.citation: Biol Reprod. 2012 May 10;86(5):147, 1-15
references.pmid: 22378763
references.citation: Mol Hum Reprod. 2009 Jun;15(6):321-33
references.pmid: 19321517
references.citation: Endocrinology. 1981 Jan;108(1):88-95
references.pmid: 6257492
references.citation: J Exp Med. 2002 Nov 4;196(9):1127-39
references.pmid: 12417624
references.citation: Recent Prog Horm Res. 1979;35:301-42
references.pmid: 229526
references.citation: J Endocrinol. 1973 Feb;56(2):321-2
references.pmid: 4703246
references.citation: Endocrinology. 1994 Jul;135(1):262-8
references.pmid: 8013361
references.citation: J Biol Chem. 1998 Mar 6;273(10):5478-83
references.pmid: 9488670
references.citation: FASEB J. 2009 Dec;23(12):4181-92
references.pmid: 19723704
references.citation: Endocr Rev. 1996 Jun;17(3):221-44
references.pmid: 8771357
references.citation: J Recept Res. 1992;12(4):413-25
references.pmid: 1334149
references.citation: Proc Natl Acad Sci U S A. 1997 Oct 14;94(21):11540-5
references.pmid: 9326645
references.citation: Endocrinology. 1991 Jul;129(1):503-10
references.pmid: 1647307
references.citation: J Clin Endocrinol Metab. 1979 Nov;49(5):687-99
references.pmid: 489711
references.citation: Proc Natl Acad Sci U S A. 1984 Mar;81(5):1549-52
references.pmid: 6324202
references.citation: Curr Mol Med. 2012 May;12(4):467-75
references.pmid: 22348614
references.citation: Br J Cancer. 2001 Nov 30;85(11):1771-80
references.pmid: 11742501
references.citation: Am J Physiol Gastrointest Liver Physiol. 2004 Jun;286(6):G1069-80
references.pmid: 14726306
references.citation: J Androl. 1998 May-Jun;19(3):343-7
references.pmid: 9639051
references.citation: J Biol Chem. 2006 Dec 15;281(50):38879-93
references.pmid: 17050526
references.citation: Biochemistry. 2007 Apr 24;46(16):4763-74
references.pmid: 17402746
references.citation: Proc Natl Acad Sci U S A. 1984 Feb;81(3):753-6
references.pmid: 6322168
references.citation: Endocr Rev. 1985 Summer;6(3):371-99
references.pmid: 3896767
references.citation: Mol Pharmacol. 1982 Jul;22(1):26-32
references.pmid: 6289073
references.citation: Pharmacol Rev. 1999 Dec;51(4):629-50
references.pmid: 10581326
references.citation: Endocrinology. 2004 Mar;145(3):1113-23
references.pmid: 14630713
references.citation: Neuroendocrinology. 1989 Mar;49(3):262-6
references.pmid: 2716952
references.citation: Nat Med. 1996 Apr;2(4):470-2
references.pmid: 8597961
references.citation: Proc Natl Acad Sci U S A. 1997 May 13;94(10):5101-6
references.pmid: 9144197
references.citation: Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3170-4
references.pmid: 1373486
references.citation: Steroids. 1997 Jan;62(1):21-8
references.pmid: 9029710
references.citation: J Biol Chem. 1990 Mar 5;265(7):3772-9
references.pmid: 2154488
references.citation: J Clin Endocrinol Metab. 1997 Dec;82(12):4243-51
references.pmid: 9398748