title: Simultaneous determination of aurantio-obtusin, chrysoobtusin, obtusin and 1-desmethylobtusin in rat plasma by UHPLC-MS/MS.
abstract: A sensitive and reliable ultra-high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-MS/MS) method was developed and validated for the simultaneous determination of four active components of Semen Cassiae extract (aurantio-obtusin, chrysoobtusin, obtusin and 1-desmethylobtusin) in rat plasma after oral administration. Chromatographic separation was achieved on an Agilent Poroshell 120 C18 column with gradient elution using a mobile phase that consisted of acetonitrile-ammonium acetate in water (30 mm) at a flow rate of 0.4 mL/min. Detection was performed by a triple-quadrupole tandem mass spectrometer in multiple reaction monitoring mode. The calibration curve was linear over a range of 3.24-1296 ng/mL for aurantio-obtusin, 0.77-618 ng/mL for chrysoobtusin, 34.55-1818 ng/mL for obtusin and 1.86-1485 ng/mL for 1-desmethylobtusin. Inter- and intra-day assay variation was <15%. All analytes were shown to be stable during all sample storage and analysis procedures.
authors.affiliation: Department of Pharmacy, School of Stomatology, Fourth Military Medical University, 145 West Changle Road, Xi'an, 710032, People's Republic of China.
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Zhang
authors.forename: Peng-yuan
authors.initials: PY
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Gu
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.forename: Shao-qing
authors.initials: SQ
authors.lastname: Wang
authors.forename: Xiao-juan
authors.initials: XJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002366:Cassia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016014:Linear Models; D008297:Male; D010936:Plant Extracts / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000880:Anthraquinones; D010936:Plant Extracts
keywords: 1-desmethylobtusin; UHPLC-MS/MS; aurantio-obtusin; chrysoobtusin; obtusin; semen cassiae