title: Characterization of a canine tetranucleotide microsatellite marker located in the first intron of the tumor necrosis factor alpha gene.
abstract: A polymorphic tetranucleotide (GAAT)n microsatellite in the first intron of the canine tumor necrosis factor alpha (TNFA) gene was characterized in this study; 139 dogs were analyzed: 22 Beagles, 26 Chihuahuas, 20 Miniature Dachshunds, 24 Miniature Poodles, 22 Pembroke Welsh Corgis and 25 Shiba Inus. We detected the presence of the 4 alleles (GAAT)5, (GAAT)6, (GAAT)7 and (GAAT)8, including 9 of the 10 expected genotypes. The expected heterozygosity (He) and the polymorphic information content (PIC) value of this microsatellite locus varied from 0.389 to 0.749 and from 0.333 to 0.682, respectively, among the 6 breeds. The allelic frequency differed greatly among breeds, but this microsatellite marker was highly polymorphic and could be a useful marker for the canine TNFA gene.
authors.affiliation: Laboratory of Veterinary Internal Medicine II, Faculty of Veterinary Medicine, Azabu University, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Watanabe
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Tanaka
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Takizawa
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Segawa
authors.forename: Sakurako
authors.initials: S
authors.lastname: Neo
authors.forename: Ryo
authors.initials: R
authors.lastname: Tsuchiya
authors.forename: Michiko
authors.initials: M
authors.lastname: Murata
authors.forename: Masaru
authors.initials: M
authors.lastname: Murakami
authors.forename: Masaharu
authors.initials: M
authors.lastname: Hisasue
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004285:Dogs / Q000235:genetics*; D007438:Introns / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D004247:DNA
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