title: Biotransformation of eugenol by suspension cultures of transgenic crown galls of Panax quinquefolium and suspension cultures of Nicotiana tabacum.
abstract: The biocatalytic ability of transgenic crown galls of Panax quinquefolium was evaluated by using eugenol (1) as a substrate and suspension cultures of Nicotiana tabacum as control system. Three biotransformed products, namely: 2-methoxy-4-(2-propenyl)phenyl-O-β-D-glucopyranoside (2, 67.11%), 2-methoxy-4-(2-propenyl)phenyl-O-β-D-glucopyranosyl (6' → 1″)-β-D-xylopyranoside (3, 2.85%) and methyl eugenol (4, 14.30%) were obtained after 5 days of administration of eugenol to the suspension cultures of transgenic crown galls of P. quinquefolium. In contrast, only one product, compound 2 (15.41%), was obtained in suspension cultures of N. tabacum after 5 days of incubation. The results indicated that the glycosylation ability of transgenic crown galls of P. quinquefolium was much higher than that of the cultured cells of N. tabacum.
authors.affiliation: Department of Pharmacy, Fujian Tumor Hospital, 350014, Fuzhou, China, apera@163.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.forename: Chunyan
authors.initials: C
authors.lastname: Yan
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhu
authors.forename: Liyan
authors.initials: L
authors.lastname: Song
authors.forename: Rongmin
authors.initials: R
authors.lastname: Yu
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