title: Three-dimensional culture of differentiated endometrial stromal cells to oligodendrocyte progenitor cells (OPCs) in fibrin hydrogel.
abstract: Neural tissue engineering is one of the most promising strategies for treatment of nerve tissue injuries. Three-dimensional (3D) environment mimics in vivo conditions for cells. 3D distribution and growth of the cells within the scaffold are both important for neural tissue engineering. In this study, endometrial stromal cell-derived oligodendrocyte progenitor cells (EnSC-derived OPCs) were cultured in fibrin gel and cell differentiation and viability were evaluated after 8 days of post-culture. The structural and mechanical characteristics of fibrin gel-like scaffold were examined with rheological analysis. EnSCs were isolated from donor tissue and were induced to OPCs with growth factors (FGF2/EGF/PDGF-AA) for 12 days, then were cultured in fibrin gel with Triiodothyronine (T3) medium for another 8 days. The viability of cells was analyzed using MTT assay for a period of 8 days culturing in a fibrin matrix. Structure of fibrin matrix and cell morphology was analyzed with SEM. TEM, immunostaining and quantitative RT-PCR was performed for OPCs markers after cell culturing in fibrin matrix. Cell viability is enhanced in fibrin matrix after 8 days. SEM and TEM show that cells are in good integration with nano-fibers. Moreover, immunohistochemistry and quantitative RT-PCR of OPCs differentiation markers showed that Olig2, Sox10, PDGFRa, CNP, and A2B5 are expressed after 8 days culturing within fibrin matrix. Fibrin can provide a suitable 3-D scaffold for EnSCs differentiated cells for the regeneration of CNS.
authors.affiliation: Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; Research Center for New Technologies in Life Science Engineering, University of Tehran, Tehran, Iran; Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran.
authors.forename: Mohammad Nabi
authors.initials: MN
authors.lastname: Asmani
authors.forename: Jafar
authors.initials: J
authors.lastname: Ai
authors.forename: Ghasem
authors.initials: G
authors.lastname: Amoabediny
authors.forename: Abbas
authors.initials: A
authors.lastname: Noroozi
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Azami
authors.forename: Somayeh
authors.initials: S
authors.lastname: Ebrahimi-Barough
authors.forename: Mona
authors.initials: M
authors.lastname: Navaei-Nigjeh
authors.forename: Armin
authors.initials: A
authors.lastname: Ai
authors.forename: Mina
authors.initials: M
authors.lastname: Jafarabadi
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D005337:Fibrin / Q000737:chemistry*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D009836:Oligodendroglia / Q000166:cytology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology* / Q000187:drug effects; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry / Q000592:standards
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D010982:Platelet-Derived Growth Factor; C075278:platelet-derived growth factor A; D016222:Fibroblast Growth Factor 2; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D004815:Epidermal Growth Factor; D005337:Fibrin
keywords: differentiation; endometrial stromal cells; fibrin gel; neural tissue engineering; oligodendrocyte progenitor cells