title: Induction of human umbilical Wharton's jelly-derived stem cells toward oligodendrocyte phenotype.
abstract: In this study, we examined phenotypic features of human Wharton's jelly mesenchymal stem cells-derived oligodendrocytes. We induced human WJMSCs to form OLs by signaling molecules including basic fibroblastic growth factor, platelet-derived growth factor-AA, and triiodothyronine hormone. Differentiated WJMSCs showed morphologic characteristics of an OL phenotype. Expression of surface markers and genes in oligodendrocyte precursor cells or OLs were analyzed by immunocytochemistry staining and real-time polymerase chain reaction, respectively. These results suggest that WJMSCs could be programmed to OLs and might provide a potential source for cell therapy in neurodegenerative disease.
authors.affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran, e.mikaeili@gmail.com.
authors.forename: Elmira
authors.initials: E
authors.lastname: Mikaeili Agah
authors.forename: Kazem
authors.initials: K
authors.lastname: Parivar
authors.forename: Mohammad
authors.initials: M
authors.lastname: Nabiuni
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Hashemi
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
mesh_terms: D005260:Female; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D055495:Neurogenesis; D009836:Oligodendroglia / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010641:Phenotype; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D014284:Triiodothyronine / Q000494:pharmacology
chemical_list: D010982:Platelet-Derived Growth Factor; C075278:platelet-derived growth factor A; D014284:Triiodothyronine; D005346:Fibroblast Growth Factors
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