title: Reduced Wall Acetylation proteins play vital and distinct roles in cell wall O-acetylation in Arabidopsis.
abstract: The Reduced Wall Acetylation (RWA) proteins are involved in cell wall acetylation in plants. Previously, we described a single mutant, rwa2, which has about 20% lower level of O-acetylation in leaf cell walls and no obvious growth or developmental phenotype. In this study, we generated double, triple, and quadruple loss-of-function mutants of all four members of the RWA family in Arabidopsis (Arabidopsis thaliana). In contrast to rwa2, the triple and quadruple rwa mutants display severe growth phenotypes revealing the importance of wall acetylation for plant growth and development. The quadruple rwa mutant can be completely complemented with the RWA2 protein expressed under 35S promoter, indicating the functional redundancy of the RWA proteins. Nevertheless, the degree of acetylation of xylan, (gluco)mannan, and xyloglucan as well as overall cell wall acetylation is affected differently in different combinations of triple mutants, suggesting their diversity in substrate preference. The overall degree of wall acetylation in the rwa quadruple mutant was reduced by 63% compared with the wild type, and histochemical analysis of the rwa quadruple mutant stem indicates defects in cell differentiation of cell types with secondary cell walls.
authors.affiliation: Joint BioEnergy Institute, Feedstocks Division, Emeryville, California 94608.
authors.forename: Yuzuki
authors.initials: Y
authors.lastname: Manabe
authors.forename: Yves
authors.initials: Y
authors.lastname: Verhertbruggen
authors.forename: Sascha
authors.initials: S
authors.lastname: Gille
authors.forename: Jesper
authors.initials: J
authors.lastname: Harholt
authors.forename: Sun-Li
authors.initials: SL
authors.lastname: Chong
authors.forename: Prashant Mohan-Anupama
authors.initials: PM
authors.lastname: Pawar
authors.forename: Ewa J
authors.initials: EJ
authors.lastname: Mellerowicz
authors.forename: Maija
authors.initials: M
authors.lastname: Tenkanen
authors.forename: Kun
authors.initials: K
authors.lastname: Cheng
authors.forename: Markus
authors.initials: M
authors.lastname: Pauly
authors.forename: Henrik Vibe
authors.initials: HV
authors.lastname: Scheller
mesh_terms: D000107:Acetylation; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D002473:Cell Wall / Q000235:genetics* / Q000378:metabolism; D005816:Genetic Complementation Test; D005936:Glucans / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009154:Mutation; D018515:Plant Leaves / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D014990:Xylans / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D005936:Glucans; C000589109:RWA1 protein, Arabidopsis; C558439:RWA2 protein, Arabidopsis; C000589111:RWA3 protein, Arabidopsis; D014990:Xylans; C029353:xyloglucan
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