title: In vivo magnetic resonance of hyperpolarized [(13)C1]pyruvate: metabolic dynamics in stimulated muscle.
abstract: The metabolic status of muscle changes according to the energetic demands of the organism. Two key regulators of these changes include exercise and insulin, with exercise eliciting catabolic expenditure within seconds and insulin enabling anabolic energy investment over minutes to hours. This study explores the potential of time-resolved hyperpolarized dynamic (13)C spectroscopy to characterize the in vivo metabolic phenotype of muscle during functional and biochemical insulin-induced stimulation of muscle. Using [(13)C1]pyruvic acid as a tracer, we find that despite the different time scales of these forms of stimulation, increases in pyruvate label transport and consumption and concomitant increases in initial rates of the tracer metabolism to lactate were observed for both stimuli. By contrast, rates of tracer metabolism to labeled alanine increased incrementally for insulin but remained unchanged following exercise-like muscle stimulation. Kinetic analysis revealed that branching of the hyperpolarized [(13)C]pyruvate tracer between lactate and alanine provides significant tissue-specific biomarkers that distinguish between anabolic and catabolic fates in vivo according to the routing of metabolites between glycolytic and amino acid pathways.
authors.affiliation: Department of Chemical Physics and.
authors.forename: Avigdor
authors.initials: A
authors.lastname: Leftin
authors.forename: Hadassa
authors.initials: H
authors.lastname: Degani
authors.forename: Lucio
authors.initials: L
authors.lastname: Frydman
mesh_terms: D000409:Alanine / Q000378:metabolism; D000465:Algorithms; D000818:Animals; D002250:Carbon Radioisotopes; D004558:Electric Stimulation; D005260:Female; D005951:Glucose Tolerance Test; D007328:Insulin / Q000494:pharmacology; D007553:Isotope Labeling; D007700:Kinetics; D019344:Lactic Acid / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051379:Mice; D008813:Mice, Inbred ICR; D009132:Muscles / Q000737:chemistry / Q000378:metabolism*; D019289:Pyruvic Acid / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D007328:Insulin; D019344:Lactic Acid; D019289:Pyruvic Acid; D000409:Alanine
keywords: dynamic nuclear polarization; electrical muscle stimulation; insulin tolerance test; magnetic resonance spectroscopy; muscle metabolism