title: Growth inhibition of Staphylococcus aureus and escherichia coli strains by neutralizing IgY antibodies from ostrich egg yolk.
abstract: Ostrich raising around the world have some key factors and farming profit depend largely on information and ability of farmers to rear these animals. Non fertilized eggs from ostriches are discharged in the reproduction season. Staphylococcus aureus and Escherichia coli are microorganisms involved in animal and human diseases. In order to optimize the use of sub products of ostrich raising, non fertilized eggs of four selected birds were utilized for development of polyclonal IgY antibodies. The birds were immunized (200ug/animal) with purified recombinant staphylococcal enterotoxin C (recSEC) and synthetic recRAP, both derived from S. aureus, and recBFPA and recEspB involved in E. coli pathogenicity, diluted in FCA injected in the braquial muscle. Two subsequent immunization steps with 21 days intervals were repeated in 0,85% saline in FIA. Blood and eggs samples were collected before and after immunization steps. Egg yolk immunoglobulins were purified by precipitation with 19% sodium sulfate and 20% ammonium sulphate methodologies. Purified IgY 50μL aliquots were incubated in 850μL BHI broth containing 50μL inoculums of five strains of S. aureus and five strains of E.coli during four hours at 37°C. Growth inhibition was evaluated followed by photometry reading (DO550nm). Egg yolk IgY preparation from hiperimmunized birds contained antibodies that inhibited significantly (p<0,05) growth of strains tested. Potential use of ostrich IgY polyclonal antibodies as a diagnostic and therapeutic tool is proposed for diseased animals.
authors.affiliation: Laboratório de Sanidade Animal, Universidade Estadual do Norte Fluminense , Campos dos Goytacazes, RJ , Brasil ; Universidade Vila Velha , Vila Velha, ES , Brasil.
authors.forename: Fernando Luiz
authors.initials: FL
authors.lastname: Tobias
authors.forename: Luize Néli Nunes
authors.initials: LN
authors.lastname: Garcia
authors.forename: Milton Masahiko
authors.initials: MM
authors.lastname: Kanashiro
authors.forename: Enrique
authors.initials: E
authors.lastname: Medina-Acosta
authors.forename: João Gato
authors.initials: JG
authors.lastname: Brom-de-Luna
authors.forename: Claudia Maria Costa
authors.initials: CM
authors.lastname: de Almeida
authors.forename: Romildo Rocha
authors.initials: RR
authors.lastname: Azevedo Junior
authors.forename: Môsar
authors.initials: M
authors.lastname: Lemos
authors.forename: Olney
authors.initials: O
authors.lastname: Vieira-da-Motta
keywords: E. coli; Ostrich IgY antibodies; S. aureus; inhibition; recombinant proteins
references.citation: Vet Res Commun. 2008 Apr;32(4):281-90
references.pmid: 18071921
references.citation: Scand J Immunol. 2003 Jun;57(6):573-82
references.pmid: 12791096
references.citation: FEMS Immunol Med Microbiol. 1999 Apr;23(4):283-8
references.pmid: 10225287
references.citation: J Bacteriol. 1988 Sep;170(9):4365-72
references.pmid: 2457579
references.citation: Arch Immunol Ther Exp (Warsz). 2009 Sep-Oct;57(5):377-82
references.pmid: 19693650
references.citation: J Med Food. 2002 Fall;5(3):159-69
references.pmid: 12495588
references.citation: Vet Microbiol. 2008 Jul 27;130(1-2):126-33
references.pmid: 18255238
references.citation: Pediatr Pulmonol. 2003 Jun;35(6):433-40
references.pmid: 12746939
references.citation: Appl Environ Microbiol. 2007 Jun;73(12):3845-9
references.pmid: 17449696
references.citation: Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1619-23
references.pmid: 7533297
references.citation: Biosci Biotechnol Biochem. 1996 May;60(5):886-8
references.pmid: 8704318
references.citation: FEMS Microbiol Lett. 2007 Feb;267(2):145-50
references.pmid: 17328113
references.citation: Vet Microbiol. 2009 Feb 2;133(4):317-22
references.pmid: 18774241
references.citation: Mol Pharmacol. 2008 May;73(5):1578-86
references.pmid: 18314496
references.citation: Vaccine. 2004 Oct 22;22(31-32):4191-202
references.pmid: 15474709
references.citation: J Appl Microbiol. 2008 Nov;105(5):1529-35
references.pmid: 19146490
references.citation: J Vet Sci. 2006 Mar;7(1):43-6
references.pmid: 16434848
references.citation: Vet Rec. 1998 Nov 21;143(21):579-84
references.pmid: 9854769
references.citation: Science. 1998 Apr 17;280(5362):438-40
references.pmid: 9545222
references.citation: Appl Microbiol. 1974 Dec;28(6):946-50
references.pmid: 4217590
references.citation: Peptides. 2001 Oct;22(10):1621-7
references.pmid: 11587790
references.citation: Vet Microbiol. 2005 Feb 25;105(3-4):245-9
references.pmid: 15708822
references.citation: J Immunol. 2001 Jan 1;166(1):669-77
references.pmid: 11123352
references.citation: Poult Sci. 2002 May;81(5):632-41
references.pmid: 12033412