title: Impact of viral factors on subcellular distribution and RNA export activity of HIV-1 rev in astrocytes 1321N1.
abstract: CNS associated cells are permissive to HIV-1 infection, but poor in virus production due to attenuated Rev activity. The temporal and the spatial distribution of Rev in human astrocyte 1321N1 and glioblastoma GO-G-CCM were monitored for explaining the reduced Rev activity and low viral production during HIV-1 infection. Rev remained localized to the nuclei of these cells upon infection, attenuating its export activity, as manifested by low copy number of RRE-containing viral-mRNA in the cytoplasm of these cells. In contrast to infection, when Rev alone was transiently expressed, it localized in the cytoplasm of 1321N1. The localization changed to the nucleus when Rev was expressed in the presence of other viral proteins through pro-viral DNA pNL4-3. This study, for the first time, revealed the impact of other HIV-1 proteins apart from host factors in regulating the subcellular localization of Rev in astrocytes and hence the fate of HIV-1 infection in these cells.
authors.affiliation: Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, Andhra Pradesh, India.
authors.forename: Atoshi
authors.initials: A
authors.lastname: Banerjee
authors.forename: Ronald
authors.initials: R
authors.lastname: Benjamin
authors.forename: Sharmistha
authors.initials: S
authors.lastname: Banerjee
mesh_terms: D001253:Astrocytes / Q000821:virology*; D045744:Cell Line, Tumor; D015694:Gene Products, rev / Q000235:genetics / Q000378:metabolism*; D015658:HIV Infections / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D009000:Monocytes / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D015694:Gene Products, rev; D012367:RNA, Viral
references.citation: J Neuroimmune Pharmacol. 2010 Mar;5(1):44-62
references.pmid: 19697136
references.citation: Cell Host Microbe. 2008 Nov 13;4(5):495-504
references.pmid: 18976975
references.citation: AIDS. 2012 Jun 19;26(10):1243-54
references.pmid: 22472854
references.citation: Curr HIV Res. 2009 Jan;7(1):91-100
references.pmid: 19149558
references.citation: Virology. 2005 Jun 5;336(2):299-307
references.pmid: 15892970
references.citation: J Virol. 1999 Oct;73(10):8279-89
references.pmid: 10482578
references.citation: Retrovirology. 2010 Apr 07;7:31
references.pmid: 20374633
references.citation: J Cell Physiol. 2008 Jan;214(1):14-9
references.pmid: 17929250
references.citation: Cell. 2004 Oct 29;119(3):381-92
references.pmid: 15507209
references.citation: Neurobiol Dis. 2013 Jan;49:128-36
references.pmid: 22926191
references.citation: Nat Protoc. 2009;4(4):495-505
references.pmid: 19300443
references.citation: PLoS One. 2010 Dec 28;5(12):e14408
references.pmid: 21203441
references.citation: Neurology. 1994 Mar;44(3 Pt 1):474-81
references.pmid: 8145918
references.citation: J Leukoc Biol. 2003 Nov;74(5):635-41
references.pmid: 12960232
references.citation: AIDS. 2010 Oct 23;24(16):2433-42
references.pmid: 20827171
references.citation: Retrovirology. 2012 Aug 13;9:65
references.pmid: 22889230
references.citation: Biochemistry. 2011 Feb 15;50(6):920-31
references.pmid: 21218853
references.citation: Arch Biochem Biophys. 1999 May 15;365(2):186-91
references.pmid: 10328811
references.citation: Adv Dent Res. 2011 Apr;23(1):13-8
references.pmid: 21441474
references.citation: AIDS. 1996 Jun;10(6):573-85
references.pmid: 8780811
references.citation: Res Virol. 1991 Mar-Jun;142(2-3):139-44
references.pmid: 1896638
references.citation: PLoS Pathog. 2009 May;5(5):e1000437
references.pmid: 19478882
references.citation: Retrovirology. 2011 Mar 14;8:17
references.pmid: 21401918
references.citation: PLoS One. 2013 Aug 01;8(8):e70250
references.pmid: 23936398
references.citation: Annu Rev Microbiol. 1998;52:491-532
references.pmid: 9891806
references.citation: Retrovirology. 2004 Oct 29;1:35
references.pmid: 15516266
references.citation: Proc Natl Acad Sci U S A. 1986 Sep;83(18):7089-93
references.pmid: 3018755
references.citation: AIDS Res Hum Retroviruses. 1992 Feb;8(2):261-8
references.pmid: 1347227
references.citation: Retrovirology. 2008 Mar 07;5:25
references.pmid: 18328091
references.citation: Arch Virol. 1994;139(3-4):365-78
references.pmid: 7832642
references.citation: J Gen Virol. 1989 Oct;70 ( Pt 10):2653-60
references.pmid: 2677235
references.citation: Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):14040-5
references.pmid: 19667186
references.citation: Microbiol Rev. 1993 Mar;57(1):183-289
references.pmid: 8464405
references.citation: Science. 2008 Feb 15;319(5865):921-6
references.pmid: 18187620
references.citation: J Virol. 1995 Apr;69(4):2159-67
references.pmid: 7884864
references.citation: J Virol. 2002 Mar;76(5):2036-42
references.pmid: 11836381
references.citation: Virol J. 2010 Dec 03;7:354
references.pmid: 21129188
references.citation: Prog Brain Res. 2001;132:721-35
references.pmid: 11545031
references.citation: EMBO J. 1990 May;9(5):1551-60
references.pmid: 2184033
references.citation: J Virol. 2004 Jul;78(14):7319-28
references.pmid: 15220405
references.citation: J Clin Invest. 1991 Jan;87(1):27-30
references.pmid: 1985101
references.citation: EMBO J. 1990 Dec;9(12):4155-60
references.pmid: 2249669
references.citation: J Virol. 1999 Jan;73(1):352-61
references.pmid: 9847339
references.citation: AIDS. 1992 Mar;6(3):273-85
references.pmid: 1373627
references.citation: Nature. 1995 Aug 10;376(6540):530-3
references.pmid: 7637788
references.citation: Curr HIV Res. 2003 Oct;1(4):463-73
references.pmid: 15049431
references.citation: Annu Rev Biochem. 1998;67:1-25
references.pmid: 9759480
references.citation: Acta Neuropathol. 1999 Nov;98(5):481-7
references.pmid: 10541871
references.citation: Ann Neurol. 1996 Jun;39(6):705-11
references.pmid: 8651642
references.citation: J Cell Sci. 2001 May;114(Pt 9):1717-29
references.pmid: 11309202
references.citation: Gene. 2005 Dec 19;363:67-76
references.pmid: 16236470
references.citation: Cell. 2008 Oct 3;135(1):49-60
references.pmid: 18854154
references.citation: Proc Natl Acad Sci U S A. 2005 Mar 15;102(11):4027-32
references.pmid: 15749819
references.citation: Protein Eng Des Sel. 2009 Dec;22(12):753-63
references.pmid: 19875511
references.citation: Mol Cell Proteomics. 2012 Apr;11(4):M111.015313
references.pmid: 22174317
references.citation: J Cell Sci. 1993 Nov;106 ( Pt 3):941-53
references.pmid: 8308076
references.citation: J Virol. 1994 Jan;68(1):93-102
references.pmid: 8254781