title: Serological survey of Ehrlichia species in dogs, horses and humans: zoonotic scenery in a rural settlement from southern Brazil.
abstract: The aims of this study were to determine the seroprevalence of Ehrlichia spp. and risk factors for exposure in a restricted population of dogs, horses, and humans highly exposed to tick bites in a Brazilian rural settlement using a commercial ELISA rapid test and two indirect immunofluorescent assays (IFA) with E. canis and E. chaffeensis crude antigens. Serum samples from 132 dogs, 16 horses and 100 humans were used. Fifty-six out of 132 (42.4%) dogs were seropositive for E. canis. Dogs > one year were more likely to be seropositive for E. canis than dogs ≤ one year (p = 0.0051). Ten/16 (62.5%) and 8/16 (50%) horses were seropositive by the commercial ELISA and IFA, respectively. Five out of 100 (5%) humans were seropositive for E. canis and E. chaffeensis. Rhipicephalus sanguineus (n = 291, 97.98%) on dogs and Amblyomma cajennense (n = 25, 96.15%) on horses were the most common ticks found. In conclusion, anti-Ehrlichia spp. antibodies were found in horses; however, the lack of a molecular characterization precludes any conclusion regarding the agent involved. Additionally, the higher seroprevalence of E. canis in dogs and the evidence of anti-Ehrlichia spp. antibodies in humans suggest that human cases of ehrlichiosis in Brazil might be caused by E. canis, or other closely related species.
authors.affiliation: Departmento de Ciências Veterinárias, Centro de Ciências Agrárias, Universidade Federal da Paraíba, 58397-000AreiaParaíba, Brazil.
authors.forename: Rafael Felipe da Costa
authors.initials: RF
authors.lastname: Vieira
authors.forename: Thállitha Samih Wischral Jayme
authors.initials: TS
authors.lastname: Vieira
authors.forename: Denise do Amaral Gomes
authors.initials: Ddo A
authors.lastname: Nascimento
authors.forename: Thiago F
authors.initials: TF
authors.lastname: Martins
authors.forename: Felipe S
authors.initials: FS
authors.lastname: Krawczak
authors.forename: Marcelo B
authors.initials: MB
authors.lastname: Labruna
authors.forename: Ramaswamy
authors.initials: R
authors.lastname: Chandrashekar
authors.forename: Mary
authors.initials: M
authors.lastname: Marcondes
authors.forename: Alexander Welker
authors.initials: AW
authors.lastname: Biondo
authors.forename: Odilon
authors.initials: O
authors.lastname: Vidotto
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D004285:Dogs / Q000276:immunology / Q000382:microbiology*; D004536:Ehrlichia / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006736:Horses / Q000276:immunology / Q000382:microbiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D048508:Rhipicephalus sanguineus / Q000382:microbiology; D012307:Risk Factors; D012424:Rural Population; D012621:Seasons; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial
references.citation: Ann N Y Acad Sci. 2006 Oct;1078:110-7
references.pmid: 17114689
references.citation: J Clin Microbiol. 1992 May;30(5):1062-6
references.pmid: 1583101
references.citation: Ticks Tick Borne Dis. 2011 Dec;2(4):213-8
references.pmid: 22108015
references.citation: Vet Parasitol. 1997 Mar;68(4):367-73
references.pmid: 9106958
references.citation: J Clin Microbiol. 1996 Sep;34(9):2133-9
references.pmid: 8862572
references.citation: Braz J Infect Dis. 2006 Feb;10(1):7-10
references.pmid: 16767308
references.citation: Vet Parasitol. 2010 Feb 10;167(2-4):108-22
references.pmid: 19811878
references.citation: Vector Borne Zoonotic Dis. 2012 Aug;12(8):705-8
references.pmid: 22607070
references.citation: Am J Vet Res. 2006 Feb;67(2):206-10
references.pmid: 16454622
references.citation: Rev Med Chil. 2003 Jan;131(1):67-70
references.pmid: 12643221
references.citation: J Clin Microbiol. 1991 Sep;29(9):2024-9
references.pmid: 1774330
references.citation: Clin Microbiol Infect. 2009 Dec;15 Suppl 2:68-9
references.pmid: 19793122
references.citation: Vet Parasitol. 2006 Sep 10;140(3-4):223-30
references.pmid: 16647817
references.citation: Vet Parasitol. 2010 Feb 10;167(2-4):136-48
references.pmid: 19819631
references.citation: Vet J. 2007 Nov;174(3):673-6
references.pmid: 17204439
references.citation: Mem Inst Oswaldo Cruz. 1961 Jul;59:115-29
references.pmid: 13861962
references.citation: Ticks Tick Borne Dis. 2010 Jun;1(2):75-99
references.pmid: 21771514
references.citation: Am J Vet Res. 2010 Dec;71(12):1443-50
references.pmid: 21117995
references.citation: J Vet Diagn Invest. 2008 May;20(3):321-4
references.pmid: 18460618
references.citation: J Appl Microbiol. 2003;94 Suppl:37S-46S
references.pmid: 12675935
references.citation: Rev Bras Parasitol Vet. 2008 Sep;17 Suppl 1:50-4
references.pmid: 20059815
references.citation: Science. 1985 Feb 1;227(4686):522-4
references.pmid: 3880925
references.citation: Int J Syst Evol Microbiol. 2001 Nov;51(Pt 6):2145-2165
references.pmid: 11760958
references.citation: Vet Parasitol. 2001 May 9;97(1):1-14
references.pmid: 11337122
references.citation: Syst Parasitol. 2009 Feb;72(2):143-57
references.pmid: 19115087
references.citation: Cad Saude Publica. 2002 Nov-Dec;18(6):1593-7
references.pmid: 12488886
references.citation: Ann N Y Acad Sci. 2003 Jun;990:126-30
references.pmid: 12860613
references.citation: Int J Syst Bacteriol. 1992 Jan;42(1):127-32
references.pmid: 1736961
references.citation: J Vet Diagn Invest. 2001 Sep;13(5):433-7
references.pmid: 11580069
references.citation: Parasit Vectors. 2008 Aug 08;1(1):25
references.pmid: 18691408
references.citation: Rev Bras Parasitol Vet. 2010 Apr-Jun;19(2):89-93
references.pmid: 20624344
references.citation: Med Clin North Am. 2002 Mar;86(2):375-92
references.pmid: 11982308
references.citation: Vet J. 2011 Mar;187(3):292-6
references.pmid: 20226700
references.citation: Braz J Infect Dis. 2004 Jun;8(3):259-62
references.pmid: 15476059
references.citation: Am J Trop Med Hyg. 2009 Feb;80(2):242-4
references.pmid: 19190221
references.citation: Mem Inst Oswaldo Cruz. 2005 Dec;100(8):853-9
references.pmid: 16444416
references.citation: Rev Bras Parasitol Vet. 2010 Jul-Sep;19(3):135-40
references.pmid: 20943015
references.citation: Rev Bras Parasitol Vet. 2011 Jan-Mar;20(1):1-12
references.pmid: 21439224
references.citation: Vector Borne Zoonotic Dis. 2010 Nov;10(9):885-8
references.pmid: 20420531
references.citation: Clin Lab Med. 2010 Mar;30(1):261-92
references.pmid: 20513551
references.citation: Vet Parasitol. 2009 May 12;161(3-4):270-5
references.pmid: 19339119
references.citation: Vector Borne Zoonotic Dis. 2010 Jun;10(5):535-7
references.pmid: 19877821
references.citation: Ann Trop Med Parasitol. 2009 Dec;103(8):719-25
references.pmid: 20030996
references.citation: Acta Vet Scand. 2010 Jan 18;52:3
references.pmid: 20082693
references.citation: Am J Trop Med Hyg. 1999 Aug;61(2):350-4
references.pmid: 10463693
references.citation: Vet Parasitol. 2006 Jun 30;139(1-3):262-6
references.pmid: 16621285
references.citation: Int J Parasitol. 2002 May;32(5):581-93
references.pmid: 11943231