title: Characterization of indigenous rhizobia from caatinga.
abstract: The aim of this study was to characterize rhizobial isolates from Cratylia mollis Mart. ex Benth, Calliandra depauperata Benth. and Mimosa tenuiflora (Willd.) Poir. by means of rhizobial colonies morphology and restriction analysis of the 16S ribosomal gene (16S rDNA-ARDRA). Nodules were collected in the field and from plants cultivated in a greenhouse experiment using Caatinga soil samples. Sixty seven isolates were described by morphological analysis. Forty seven representative isolates were used for ARDRA analysis using seven restriction enzymes. We observed high diversity of both slow and fast-growing rhizobia that formed three morpho-physiological clusters. A few fast-growing isolates formed a group of strains of the Bradyrhizobium type; however, most of them diverged from the B. japonicum and B. elkanii species. Cratylia mollis nodule isolates were the most diverse, while all Mimosa tenuiflora isolates displayed fast growth with no pH change and were clustered into groups bearing 100% similarity, according to ARDRA results.
authors.affiliation: Fundação Instituto Brasileiro de Geografia e Estatística , Rio de Janeiro, RJ , Brasil.
authors.forename: Fernanda Cíntia
authors.initials: FC
authors.lastname: Pires E Teixeira
authors.forename: Wardsson Lustrino
authors.initials: WL
authors.lastname: Borges
authors.forename: Gustavo Ribeiro
authors.initials: GR
authors.lastname: Xavier
authors.forename: Norma Gouvêa
authors.initials: NG
authors.lastname: Rumjanek
keywords: Calliandra depauperata; Cratylia mollis; Mimosa tenuiflora; PCR-ARDRA; diversity
references.citation: J Bacteriol. 1991 Apr;173(7):2271-7
references.pmid: 2007551
references.citation: Appl Environ Microbiol. 2005 Nov;71(11):7461-71
references.pmid: 16269788
references.citation: Microb Ecol. 2007 Feb;53(2):270-84
references.pmid: 17265000
references.citation: Syst Appl Microbiol. 2006 Apr;29(3):197-206
references.pmid: 16564956
references.citation: Int J Syst Evol Microbiol. 2007 May;57(Pt 5):1055-1059
references.pmid: 17473258
references.citation: Syst Appl Microbiol. 2005 Jan;28(1):57-65
references.pmid: 15709366
references.citation: Appl Environ Microbiol. 1994 Aug;60(8):2772-8
references.pmid: 16349348
references.citation: Nature. 2001 Jun 21;411(6840):948-50
references.pmid: 11418858