title: Co-metabolism of DDT by the newly isolated bacterium, Pseudoxanthomonas sp. wax.
abstract: Microbial degradation of 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT) is the most promising way to clean up DDT residues found in the environment. In this paper, a bacterium designated as wax, which was capable of co-metabolizing DDT with other carbon sources, was isolated from a long-term DDT-contaminated soil sample by an enrichment culture technique. The new isolate was identified as a member of the Pseudoxanthomonas sp., based on its morphological, physiological and biochemical properties, as well as by 16S rRNA gene analysis. In the presence of 100 mg l(-1) glucose, the wax strain could degrade over 95% of the total DDT, at a concentration of 20 mg l(-1), in 72 hours, and could degrade over 60% of the total DDT, at a concentration of 100 mg l(-1), in 144 hours. The wax strain had the highest degradation efficiency among all of the documented DDT-degrading bacteria. The wax strain could efficiently degrade DDT at temperatures ranging from 20 to 37°C, and with initial pH values ranging from 7 to 9. The bacterium could also simultaneously co-metabolize 1,1-dichloro-2,2-bis(p-chlorophenyl)ethane (DDD), 2,2-bis(p-chlorophenyl)-1,1-dichlorethylene (DDE), and other organochlorine compounds. The wax strain could also completely remove 20 mg kg(-1) of DDT from both sterile and non-sterile soils in 20 days. This study demonstrates the significant potential use of Pseudoxanthomonas sp. wax for the bioremediation of DDT in the environment.
authors.affiliation: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University , 210095, Nanjing, Jiangsu Province , China.
authors.forename: Guangli
authors.initials: G
authors.lastname: Wang
authors.forename: Ji
authors.initials: J
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Liang
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.forename: Shunpeng
authors.initials: S
authors.lastname: Li
authors.forename: Jiandong
authors.initials: J
authors.lastname: Jiang
keywords: Bioremediation; Co-metabolism; DDT; Pseudoxanthomonas sp.
references.citation: Bull Environ Contam Toxicol. 1989 Jan;42(1):45-9
references.pmid: 2924005
references.citation: J Agric Food Chem. 1973 Jul-Aug;21(4):511-9
references.pmid: 4578152
references.citation: J Clin Microbiol. 1990 Sep;28(9):1942-6
references.pmid: 2095137
references.citation: Arch Microbiol. 1998 Dec;171(1):44-9
references.pmid: 9871018
references.citation: Nucleic Acids Res. 1988 Feb 11;16(3):1215
references.pmid: 3344216
references.citation: Science. 1995 Sep 29;269(5232):1851-4
references.pmid: 7569923
references.citation: Appl Microbiol. 1967 May;15(3):569-74
references.pmid: 6035049
references.citation: Bull World Health Organ. 1965;33(4):471-7
references.pmid: 5294993
references.citation: Appl Microbiol Biotechnol. 1990 Oct;34(1):108-15
references.pmid: 1366971
references.citation: Biomed Environ Mass Spectrom. 1989 Sep;18(9):741-52
references.pmid: 2790260
references.citation: Biodegradation. 2007 Aug;18(4):403-12
references.pmid: 17091349
references.citation: Appl Environ Microbiol. 1994 Jan;60(1):51-5
references.pmid: 8117093
references.citation: Nature. 1995 Jun 15;375(6532):581-5
references.pmid: 7791873
references.citation: Sci Total Environ. 1994 Aug 15;153(1-2):29-49
references.pmid: 7939620
references.citation: Mol Biol Evol. 1987 Jul;4(4):406-25
references.pmid: 3447015
references.citation: Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
references.pmid: 9396791
references.citation: Appl Environ Microbiol. 2003 Sep;69(9):5198-206
references.pmid: 12957902