title: The sealing ability of MTA apical plugs exposed to a phosphate-buffered saline.
abstract: OBJECTIVE
  The aim of this study was to evaluate the influence of exposure of the mineral trioxide aggregate (MTA) - with and without calcium chloride (CaCl2) - to phosphate-buffered saline (PBS) on the apical microleakage using a glucose leakage system.
  
  
  METHODS
  Sixty root segments were randomly divided into 4 experimental groups (n=15). After resecting the apical segments and enlarging the canals with Gates-Glidden drills, the apical cavities were filled with MTA with or without CaCl2 and the root canals were dressed with a moistened cotton pellet or PBS, as follows: 1) MTA/cotton pellet; 2) MTA/PBS; 3) MTA+10%CaCl2/cotton pellet; 4) MTA+10%CaCl2/PBS. All root segments were introduced in floral foams moistened with PBS. After 2 months, all root segments were prepared to evaluate the glucose leakage along the apical plugs. The amount of glucose leakage was measured following an enzymatic reaction and quantified by a spectrophotometer. Four roots were used as controls. The data were analyzed using Kruskal-Wallis and Mann-Whitney tests (p<0.05).
  
  
  RESULTS
  There were no differences between groups 1 and 2 (p>0.05), and 3 and 4 (p>0.05). The addition of CaCl2 to the MTA significantly decreased its sealing ability (p<0.05).
  
  
  CONCLUSIONS
  The interaction with PBS did not improve the MTA sealing ability. The addition of CaCl2 to the MTA negatively influenced the apical seal.
authors.affiliation: Department of Endodontics, School of Dentistry, Federal University of Santa Catarina, FlorianópolisSC, Brazil.
authors.forename: Josiane de
authors.initials: Jd
authors.lastname: Almeida
authors.forename: Ana Maria Hecke
authors.initials: AM
authors.lastname: Alves
authors.forename: Roberto Ferreira de
authors.initials: RF
authors.lastname: Melo
authors.forename: Mara Cristina Santos
authors.initials: MC
authors.lastname: Felippe
authors.forename: Eduardo Antunes
authors.initials: EA
authors.lastname: Bortoluzzi
authors.forename: Cleonice da Silveira
authors.initials: Cda S
authors.lastname: Teixeira
authors.forename: Wilson Tadeu
authors.initials: WT
authors.lastname: Felippe
mesh_terms: D017607:Aluminum Compounds / Q000737:chemistry*; D002122:Calcium Chloride / Q000737:chemistry*; D017610:Calcium Compounds / Q000737:chemistry*; D003763:Dental Leakage; D003786:Dental Pulp Cavity / Q000187:drug effects*; D004338:Drug Combinations; D005947:Glucose / Q000032:analysis; D006801:Humans; D008422:Materials Testing; D010087:Oxides / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry*; D011897:Random Allocation; D012387:Root Canal Filling Materials / Q000737:chemistry*; D018915:Root Canal Preparation / Q000379:methods; D017640:Silicates / Q000737:chemistry*; D013053:Spectrophotometry; D018709:Statistics, Nonparametric; D013499:Surface Properties; D013997:Time Factors
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D010710:Phosphates; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D005947:Glucose; D002122:Calcium Chloride
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