title: Spectral Phasor approach for fingerprinting of photo-activatable fluorescent proteins Dronpa, Kaede and KikGR.
abstract: The phasor global analysis algorithm is common for fluorescence lifetime applications, but has only been recently proposed for spectral analysis. Here the phasor representation and fingerprinting is exploited in its second harmonic to determine the number and spectra of photo-activated states as well as their conversion dynamics. We follow the sequence of photo-activation of proteins over time by rapidly collecting multiple spectral images. The phasor representation of the cumulative images provides easy identification of the spectral signatures of each photo-activatable protein.
authors.forename: Francesco
authors.initials: F
authors.lastname: Cutrale
authors.forename: Anya
authors.initials: A
authors.lastname: Salih
authors.forename: Enrico
authors.initials: E
authors.lastname: Gratton
references.citation: J Biol Chem. 2012 Oct 12;287(42):35047-35056
references.pmid: 22904329
references.citation: Biophys J. 2010 Mar 3;98(5):911-21
references.pmid: 20197045
references.citation: Science. 2004 Nov 19;306(5700):1370-3
references.pmid: 15550670
references.citation: Biophys J. 2008 Jan 15;94(2):L14-6
references.pmid: 17981902
references.citation: Physiol Rev. 2010 Jul;90(3):1103-63
references.pmid: 20664080
references.citation: Proc Natl Acad Sci U S A. 2005 Jul 5;102(27):9511-6
references.pmid: 15972810
references.citation: Microsc Res Tech. 2012 Mar;75(3):271-81
references.pmid: 21858900
references.citation: J Biomed Opt. 2012 Apr;17(4):046012
references.pmid: 22559690
references.citation: Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12651-6
references.pmid: 12271129
references.citation: Photochem Photobiol Sci. 2006 Jun;5(6):567-76
references.pmid: 16761085
references.citation: Opt Express. 2012 Jun 4;20(12):12729-41
references.pmid: 22714302
references.citation: EMBO Rep. 2005 Mar;6(3):233-8
references.pmid: 15731765
references.citation: J Biol Chem. 2011 Apr 29;286(17):15032-42
references.pmid: 21388960
references.citation: PLoS One. 2011;6(6):e21127
references.pmid: 21698153
references.citation: Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):13582-7
references.pmid: 21808026
references.citation: Science. 2006 Sep 15;313(5793):1642-5
references.pmid: 16902090