title: Rosmarinus officinalis essential oil: antiproliferative, antioxidant and antibacterial activities.
abstract: The aim of this work was to investigate and compare the antiproliferative, antioxidant and antibacterial activities of Rosmarinus officinalis essential oil, native to Pakistan. The essential oil content from the leaves of R. officinalis was 0.93 g 100g(-1). The GC and GC-MS analysis revealed that the major components determined in R. officinalis essential oil were 1,8-cineol (38.5%), camphor (17.1%), α-pinene (12.3%), limonene (6.23%), camphene (6.00%) and linalool (5.70%). The antiproliferative activity was tested against two cancer (MCF-7 and LNCaP) and one fibroblast cell line (NIH-3T3) using the MTT assay, while, the antioxidant activity was evaluated by the reduction of 2, 2-diphenyl-1-picryl hydrazyl (DPPH) and measuring percent inhibition of peroxidation in linoleic acid system. The disc diffusion and modified resazurin microtitre-plate assays were used to evaluate the inhibition zones (IZ) and minimum inhibitory concentration (MIC) of R. officinalis essential oil, respectively. It is concluded from the results that Rosmarinus officinalis essential oil exhibited antiproliferative, antioxidant and antibacterial activities.
authors.affiliation: Department of Chemistry, Government College University , Faisalabad , Pakistan ; Centre of Molecular Biosciences, Institute of Biomedical Sciences Research, University of Ulster , Coleraine, BT52 1SA , UK.
authors.forename: Abdullah Ijaz
authors.initials: AI
authors.lastname: Hussain
authors.forename: Farooq
authors.initials: F
authors.lastname: Anwar
authors.forename: Shahzad Ali Shahid
authors.initials: SA
authors.lastname: Chatha
authors.forename: Abdul
authors.initials: A
authors.lastname: Jabbar
authors.forename: Shahid
authors.initials: S
authors.lastname: Mahboob
authors.forename: Poonam Singh
authors.initials: PS
authors.lastname: Nigam
keywords: 1,8-cienol; Antibacterial; Antioxidant; Resazurin assay; antiproliferative
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