title: Screening and optimization of pectin lyase and polygalacturonase activity from ginseng pathogen Cylindrocarpon Destructans.
abstract: Cylindrocarpon destructans isolated from ginseng field was found to produce pectinolytic enzymes. A Taguchi's orthogonal array experimental design was applied to optimize the preliminary production of polygalacturonase (PG) and pectin lyase (PL) using submerged culture condition. This method was applied to evaluate the significant parameters for the production of enzymes. The process variables were pH, pectin concentration, incubation time and temperature. Optimization of process parameters resulted in high levels of enzyme (PG and PL) production after ten days of incubation at a pH of 5.0 at 25°C in the presence of 1.5% pectin. Among different nitrogen sources, urea and peptone showed high production of PG and PL, respectively. The enzyme production and mycelial growth seems to have direct influence on the culture conditions; therefore, at stationary state high enzyme production and mycelial growth were obtained than agitation state. Along with this, optimization of enzyme activity was also determined using various physiological parameters like, temperature, incubation time and pH. Taguchi's data was also analyzed using one step ANOVA statistical method.
authors.affiliation: Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University , Suwon 449-701 , Korea.
authors.forename: Gayathri
authors.initials: G
authors.lastname: Sathiyaraj
authors.forename: Sathiyaraj
authors.initials: S
authors.lastname: Srinivasan
authors.forename: Ho-Bin
authors.initials: HB
authors.lastname: Kim
authors.forename: Sathiyamoorthy
authors.initials: S
authors.lastname: Subramaniyam
authors.forename: Ok Ran
authors.initials: OR
authors.lastname: Lee
authors.forename: Yeon-Ju
authors.initials: YJ
authors.lastname: Kim
authors.forename: Deok Chun
authors.initials: DC
authors.lastname: Yang
keywords: C. destructans; ginseng; pectin lyases; polygalacturonase
references.citation: Phytopathology. 2003 Dec;93(12):1533-42
references.pmid: 18943617
references.citation: Appl Environ Microbiol. 1989 Jun;55(6):1612-6
references.pmid: 16347954
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: Phytopathology. 2005 Dec;95(12):1381-90
references.pmid: 18943548
references.citation: J Ind Microbiol Biotechnol. 2008 Sep;35(9):1001-5
references.pmid: 18504630
references.citation: Bioresour Technol. 2008 Sep;99(14):6697-701
references.pmid: 17996445
references.citation: Appl Environ Microbiol. 1990 Dec;56(12):3755-9
references.pmid: 16348377