title: Poly (lactide-co-glycolide) nano-encapsulation of chelidonine, an active bioingredient of greater celandine (Chelidonium majus), enhances its ameliorative potential against cadmium induced oxidative stress and hepatic injury in mice.
abstract: This study evaluates the possible protective potentials of chelidonine and its poly lactide-co-glycolide (PLGA) encapsulated nano-form against cadmium chloride (CdCl₂) induced oxidative stress and hepatotoxicity in mice, ex vivo and in vivo. Acute exposure to CdCl₂ (1.0 mg/kg b.w; i.p., twice a week for 30 days) generated oxidative stress in mice through accumulation of reactive oxygen species and increased lipid peroxidation, and levels of certain liver marker enzymes (ALT, AST, ALP) with decrease in levels of GSH and certain other antioxidant enzymes (SOD, CAT, GR) in liver. Treatment with nano-chelidonine for 30 days after CdCl₂ intoxication significantly reduced oxidative stress and lipid peroxidation and restored levels of GSH, cholesterol, triglyceride and antioxidant enzymes, showing ameliorative changes in histopathology of liver. Expression pattern of certain inflammatory and apoptotic signal proteins also indicated better hepato-protective abilities of nano-chelidonine, making it a more suitable protective drug than chelidonine against cadmium toxicity in mice.
authors.affiliation: Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India.
authors.forename: Avijit
authors.initials: A
authors.lastname: Paul
authors.forename: Jayeeta
authors.initials: J
authors.lastname: Das
authors.forename: Sreemanti
authors.initials: S
authors.lastname: Das
authors.forename: Asmita
authors.initials: A
authors.lastname: Samadder
authors.forename: Anisur Rahman
authors.initials: AR
authors.lastname: Khuda-Bukhsh
mesh_terms: D000465:Algorithms; D000818:Animals; D053119:Benzophenanthridines / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D019256:Cadmium Chloride / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D002105:Cadmium Poisoning / Q000517:prevention & control*; D002470:Cell Survival / Q000187:drug effects; D028281:Chelidonium / Q000737:chemistry*; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D004339:Drug Compounding / Q000379:methods*; D005079:Excipients; D022781:Hepatocytes / Q000187:drug effects; D007150:Immunohistochemistry; D007202:Indicators and Reagents; D019344:Lactic Acid / Q000737:chemistry*; D008027:Light; D051379:Mice; D053758:Nanoparticles; D036103:Nanotechnology; D018384:Oxidative Stress / Q000187:drug effects*; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012542:Scattering, Radiation; D013054:Spectrophotometry, Atomic
chemical_list: D053119:Benzophenanthridines; D005079:Excipients; D007202:Indicators and Reagents; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C062047:chelidonine; D019256:Cadmium Chloride
keywords: Cadmium; Chelidonine; Hepatotoxicity; Oxidative stress; PLGA nano-encapsulation; Signal proteins