title: Human papillomavirus infection in women from tlaxcala, Mexico.
abstract: Cervical cancer is an important health problem in women living in developing countries. Infection with some genotypes of human papillomavirus (HPV) is the most important risk factor associated with cervical cancer. Little information exists about HPV genotype distribution in rural and suburban regions of Mexico. Thus, we determined the prevalence of HPV genotypes in women from Tlaxcala, one of the poorest states in central Mexico, and we evaluated age infection prevalence and risk factors associated with cervical neoplasm. A cross-sectional study was conducted in 236 women seeking gynecological care at the Mexican Institute for Social Security in Tlaxcala, Mexico. Cervical scrapings were diagnosed as normal, low-grade, and high-grade squamous intraepithelial lesions (LGSIL, HGSIL). Parallel samples were used to detect HPV genotypes by PCR assays using type-specific primers for HPV 6, 11, 16, 18, and 31. An epidemiological questionnaire was applied. Prevalence of HPV infection was 31.3%. From the infected samples, prevalence of HPV 16 was 45.9%; HPV 18, 31.1%; HPV 31, 16.2%; HPV 6, 10.8%; HPV 11, 6.7%. With regard to age, the highest HPV prevalence (43.5%) was found in the 18- to 24-year-old group and the lowest (19%) in the 45- to 54-year-old group. None of the risk factors showed association with cervical neoplasia grade. HPV 16 was the most common in cervical lesions. HPV was present in 22% of normal samples and, of these, 82.6% represented high-risk HPVs. Tlaxcala showed HPV prevalence comparable to that of the largest cities in Mexico, with higher prevalence for HPV 31.
authors.affiliation: Laboratorio de Biología Molecular y Virología, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social , Metepec, Puebla , México ; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla , Puebla, Pue. , México.
authors.forename: Noé
authors.initials: N
authors.lastname: Velázquez-Márquez
authors.forename: Lucio
authors.initials: L
authors.lastname: Jaime Jiménez-Aranda
authors.forename: Patricia
authors.initials: P
authors.lastname: Sánchez-Alonso
authors.forename: Gerardo
authors.initials: G
authors.lastname: Santos-López
authors.forename: Julio
authors.initials: J
authors.lastname: Reyes-Leyva
authors.forename: Verónica
authors.initials: V
authors.lastname: Vallejo-Ruiz
keywords: Epidemiology; Human papillomavirus; Mexico; PCR; Squamous intraepithelial lesions
references.citation: Cancer Epidemiol Biomarkers Prev. 2005 Oct;14(10):2439-45
references.pmid: 16214929
references.citation: Lancet Infect Dis. 2007 Jul;7(7):453-9
references.pmid: 17597569
references.citation: Int J Cancer. 2001 Feb 1;91(3):412-20
references.pmid: 11169968
references.citation: N Engl J Med. 2003 Feb 6;348(6):518-27
references.pmid: 12571259
references.citation: Int J Gynecol Cancer. 2006 May-Jun;16(3):1041-7
references.pmid: 16803483
references.citation: Circ Res. 1998 Jun 15;82(11):1224-30
references.pmid: 9633921
references.citation: Lancet. 2005 Sep 17-23;366(9490):991-8
references.pmid: 16168781
references.citation: N Engl J Med. 1999 Nov 25;341(22):1633-8
references.pmid: 10572150
references.citation: JAMA. 2002 Apr 24;287(16):2114-9
references.pmid: 11966386
references.citation: Salud Publica Mex. 2008 Jan-Feb;50(1):49-58
references.pmid: 18297182
references.citation: Salud Publica Mex. 2003;45 Suppl 3:S306-14
references.pmid: 14746023
references.citation: Braz J Microbiol. 2008 Jul;39(3):573-8
references.pmid: 24031268
references.citation: Salud Publica Mex. 2004 Jan-Feb;46(1):7-15
references.pmid: 15053391
references.citation: J Clin Pathol. 2002 Apr;55(4):244-65
references.pmid: 11919208
references.citation: Cancer Epidemiol Biomarkers Prev. 2006 Jul;15(7):1274-80
references.pmid: 16835323
references.citation: Virology. 2004 Jun 20;324(1):17-27
references.pmid: 15183049
references.citation: J Clin Microbiol. 1990 Dec;28(12):2739-43
references.pmid: 2177751
references.citation: Int J Infect Dis. 2009 Nov;13(6):690-5
references.pmid: 19147384
references.citation: J Natl Cancer Inst. 2000 Mar 15;92(6):464-74
references.pmid: 10716964
references.citation: BMC Infect Dis. 2006 Feb 20;6:27
references.pmid: 16504014