title: Changes in WNT signaling-related gene expression associated with development and cloning in bovine extra-embryonic and endometrial tissues during the peri-implantation period.
abstract: We determined if somatic cell nuclear transfer (SCNT) cloning is associated with WNT-related gene expression in cattle development, and if the expression of genes in the WNT pathway changes during the peri-implantation period. Extra-embryonic and endometrial tissues were collected at gestation days 18 and 34 (d18, d34). WNT5A, FZD4, FZD5, LRP5, CTNNB1, GNAI2, KDM1A, BCL2L1, and SFRP1 transcripts were localized in extra-embryonic tissue, whereas SFRP1 and DKK1 were localized in the endometrium. There were no differences in the localization of these transcripts in extra-embryonic tissue or endometrium from SCNT or artificial insemination (AI) pregnancies. Expression levels of WNT5A were 11-fold greater in the allantois of SCNT than AI samples. In the trophoblast, expression of WNT5A, FZD5, CTNNB1, and DKK1 increased significantly from d18 to d34, whereas expression of KDM1A and SFRP1 decreased, indicating that implantation is associated with major changes in WNT signaling. SCNT was associated with altered WNT5A expression in trophoblasts, with levels increasing 2.3-fold more in AI than SCNT conceptuses from d18 to d34. In the allantois, expression of WNT5A increased 6.3-fold more in SCNT than AI conceptuses from d18 to d34. Endometrial tissue expression levels of the genes tested did not differ between AI or SCNT pregnancies, although expression of individual genes showed variation across developmental stages. Our results demonstrate that SCNT is associated with altered expression of specific WNT-related genes in extra-embryonic tissue in a time- and tissue-specific manner. The pattern of gene expression in the WNT pathway suggests that noncanonical WNT signal transduction is important for implantation of cattle conceptuses.
authors.affiliation: Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois.
authors.forename: Fernando H
authors.initials: FH
authors.lastname: Biase
authors.forename: Chanaka
authors.initials: C
authors.lastname: Rabel
authors.forename: Michel
authors.initials: M
authors.lastname: Guillomot
authors.forename: Olivier
authors.initials: O
authors.lastname: Sandra
authors.forename: Kalista
authors.initials: K
authors.lastname: Andropolis
authors.forename: Colleen
authors.initials: C
authors.lastname: Olmstead
authors.forename: Rosane
authors.initials: R
authors.lastname: Oliveira
authors.forename: Richard
authors.initials: R
authors.lastname: Wallace
authors.forename: Daniel
authors.initials: D
authors.lastname: Le Bourhis
authors.forename: Christophe
authors.initials: C
authors.lastname: Richard
authors.forename: Evelyne
authors.initials: E
authors.lastname: Campion
authors.forename: Aurélie
authors.initials: A
authors.lastname: Chaulot-Talmon
authors.forename: Corinne
authors.initials: C
authors.lastname: Giraud-Delville
authors.forename: Géraldine
authors.initials: G
authors.lastname: Taghouti
authors.forename: Hélène
authors.initials: H
authors.lastname: Jammes
authors.forename: Isabelle
authors.initials: I
authors.lastname: Hue
authors.forename: Jean Paul
authors.initials: JP
authors.lastname: Renard
authors.forename: Harris A
authors.initials: HA
authors.lastname: Lewin
mesh_terms: D000482:Allantois / Q000378:metabolism; D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002417:Cattle; D019976:Cloning, Organism; D010064:Embryo Implantation / Q000235:genetics*; D004717:Endometrium / Q000196:embryology* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D007315:Insemination, Artificial; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D053652:Nuclear Transfer Techniques; D051153:Wnt Proteins / Q000096:biosynthesis / Q000378:metabolism; D060449:Wnt Signaling Pathway / Q000235:genetics*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D051153:Wnt Proteins