title: Four new diterpenoids from the roots of Euphorbia fischeriana.
abstract: Four new diterpenoids (1,4,5,9), together with 7 known diterpenoids (2,3,6-8,10,11), were isolated from the roots to Euphorbia fischeriana. On the basis of 1D and 2D NMR, HR-ESI-MS spectroscopic analysis, structures of the new compounds were elucidated as 11β-hydroxy-8,14-epoxy-ent-abieta-13(15)-en-16,12-olide (1), 3,20-dihydroxy-ent-1(10), 15-rosadiene (4), 3,7-dihydroxy-ent-1(10), 15-rosadiene (5), ingenol 6,7-epoxy-3- tetradecanoate (9). The compounds isolated were evaluated for their cytotoxicity against four cancer cell lines (A549, BEL7402, HCT116, and MDA-MB-231). Three ingenol diterpenoids (9-11) showed significant cytotoxicity against A549 with IC50 value of 3.35, 2.85, 2.88 μg/mL, respectively.
authors.affiliation: School of Life Sciences & Technology, Tongji University, Shanghai 200092, PR China. Electronic address: hbwang@tongji.edu.cn.
authors.forename: Hong-Bing
authors.initials: HB
authors.lastname: Wang
authors.affiliation: School of Life Sciences & Technology, Tongji University, Shanghai 200092, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: School of Life Sciences & Technology, Tongji University, Shanghai 200092, PR China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: School of Life Sciences & Technology, Tongji University, Shanghai 200092, PR China; School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, PR China.
authors.forename: Xiao-Yang
authors.initials: XY
authors.lastname: Wang
authors.affiliation: School of Life Sciences & Technology, Tongji University, Shanghai 200092, PR China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Liu
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, PR China.
authors.forename: Ling-Jiang
authors.initials: LJ
authors.lastname: Tong
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D028481:Euphorbia / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D009369:Neoplasms / Q000188:drug therapy*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D018517:Plant Roots / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D010936:Plant Extracts; C000588652:ingenol 6,7-epoxy-3-tetradecanoate; C008125:ingenol
keywords: Cytotoxicity; Diterpenoids; Euphorbia fischeriana