title: Identification and characterization of an inhibitor of trichothecene 3-O-acetyltransferase, TRI101, by the chemical array approach.
abstract: Trichothecene 3-O-acetyltransferase (TRI101) is an indispensable enzyme for the biosynthesis of trichothecenes, a group of mycotoxins produced by Fusarium graminearum. In this study, an inhibitor of TRI101 was identified by chemical array analysis using compounds from the RIKEN Natural Products Depository (NPDepo) library. Although the addition of the identified enzyme inhibitor to the fungal culture did not inhibit trichothecene production, it can serve as a candidate lead compound in the development of a mycotoxin inhibitor that inactivates fungal defense mechanisms.
authors.affiliation: Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Nakajima
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Kawamura
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Maeda
authors.forename: Hinayo
authors.initials: H
authors.lastname: Ichikawa
authors.forename: Takayuki
authors.initials: T
authors.lastname: Motoyama
authors.forename: Yasumitsu
authors.initials: Y
authors.lastname: Kondoh
authors.forename: Tamio
authors.initials: T
authors.lastname: Saito
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Minoru
authors.initials: M
authors.lastname: Yoshida
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Osada
authors.forename: Makoto
authors.initials: M
authors.lastname: Kimura
mesh_terms: D000123:Acetyltransferases / Q000037:antagonists & inhibitors*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D013997:Time Factors; D014631:Valerates / Q000737:chemistry / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D014631:Valerates; D000123:Acetyltransferases; C110205:trichothecene 3-O-acetyltransferase