title: Structure of a helicase-helicase loader complex reveals insights into the mechanism of bacterial primosome assembly.
abstract: During the assembly of the bacterial loader-dependent primosome, helicase loader proteins bind to the hexameric helicase ring, deliver it onto the oriC DNA and then dissociate from the complex. Here, to provide a better understanding of this key process, we report the crystal structure of the ~570-kDa prepriming complex between the Bacillus subtilis loader protein and the Bacillus stearothermophilus helicase, as well as the helicase-binding domain of primase with a molar ratio of 6:6:3 at 7.5 Å resolution. The overall architecture of the complex exhibits a three-layered ring conformation. Moreover, the structure combined with the proposed model suggests that the shift from the 'open-ring' to the 'open-spiral' and then the 'closed-spiral' state of the helicase ring due to the binding of single-stranded DNA may be the cause of the loader release.
authors.affiliation: 1] Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA [2] Howard Hughes Medical Institute, New Haven, Connecticut 06510, USA.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: William K
authors.initials: WK
authors.lastname: Eliason
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Steitz
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D001407:Bacillus / Q000201:enzymology*; D002850:Chromatography, Gel; D004265:DNA Helicases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D008958:Models, Molecular; D017434:Protein Structure, Tertiary; D055672:Static Electricity
chemical_list: D004277:DNA, Single-Stranded; D000255:Adenosine Triphosphate; D004265:DNA Helicases
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