title: Interleukin-10 (IL-10) inhibits Borrelia burgdorferi-induced IL-17 production and attenuates IL-17-mediated Lyme arthritis.
abstract: Previous studies have shown that cells and cytokines associated with interleukin-17 (IL-17)-driven inflammation are involved in the arthritic response to Borrelia burgdorferi infection. Here, we report that IL-17 is a contributing factor in the development of Lyme arthritis and show that its production and histopathological effects are regulated by interleukin-10 (IL-10). Spleen cells obtained from B. burgdorferi-infected, "arthritis-resistant" wild-type C57BL/6 mice produced low levels of IL-17 following stimulation with the spirochete. In contrast, spleen cells obtained from infected, IL-10-deficient C57BL/6 mice produced a significant amount of IL-17 following stimulation with B. burgdorferi. These mice developed significant arthritis, including erosion of the bones in the ankle joints. We further show that treatment with antibody to IL-17 partially inhibited the significant hind paw swelling and histopathological changes observed in B. burgdorferi-infected, IL-10-deficient mice. Taken together, these findings provide additional evidence of a role for IL-17 in Lyme arthritis and reveal an additional regulatory target of IL-10 following borrelial infection.
authors.affiliation: Department of Biomedical Sciences, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, USA.
authors.forename: Emily S
authors.initials: ES
authors.lastname: Hansen
authors.forename: Velinka
authors.initials: V
authors.lastname: Medić
authors.forename: Joseph
authors.initials: J
authors.lastname: Kuo
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Warner
authors.forename: Ronald F
authors.initials: RF
authors.lastname: Schell
authors.forename: Dean T
authors.initials: DT
authors.lastname: Nardelli
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D001168:Arthritis / Q000276:immunology*; D025065:Borrelia burgdorferi / Q000276:immunology*; D002478:Cells, Cultured; D007249:Inflammation / Q000276:immunology; D016753:Interleukin-10 / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D020381:Interleukin-17 / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*; D008193:Lyme Disease / Q000276:immunology / Q000382:microbiology / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D058504:Th17 Cells / Q000276:immunology / Q000378:metabolism
chemical_list: D000906:Antibodies; D020381:Interleukin-17; D016753:Interleukin-10
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