title: Topographical body fat distribution links to amino acid and lipid metabolism in healthy obese women [corrected].
abstract: Visceral adiposity is increasingly recognized as a key condition for the development of obesity related disorders, with the ratio between visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) reported as the best correlate of cardiometabolic risk. In this study, using a cohort of 40 obese females (age: 25-45 y, BMI: 28-40 kg/m(2)) under healthy clinical conditions and monitored over a 2 weeks period we examined the relationships between different body composition parameters, estimates of visceral adiposity and blood/urine metabolic profiles. Metabonomics and lipidomics analysis of blood plasma and urine were employed in combination with in vivo quantitation of body composition and abdominal fat distribution using iDXA and computerized tomography. Of the various visceral fat estimates, VAT/SAT and VAT/total abdominal fat ratios exhibited significant associations with regio-specific body lean and fat composition. The integration of these visceral fat estimates with metabolic profiles of blood and urine described a distinct amino acid, diacyl and ether phospholipid phenotype in women with higher visceral fat. Metabolites important in predicting visceral fat adiposity as assessed by Random forest analysis highlighted 7 most robust markers, including tyrosine, glutamine, PC-O 44∶6, PC-O 44∶4, PC-O 42∶4, PC-O 40∶4, and PC-O 40∶3 lipid species. Unexpectedly, the visceral fat associated inflammatory profiles were shown to be highly influenced by inter-days and between-subject variations. Nevertheless, the visceral fat associated amino acid and lipid signature is proposed to be further validated for future patient stratification and cardiometabolic health diagnostics.
authors.affiliation: Metabolomics and Biomarkers, Nestec Ltd., Nestle Research Center, Lausanne, Switzerland.
authors.forename: Francois-Pierre J
authors.initials: FP
authors.lastname: Martin
authors.forename: Ivan
authors.initials: I
authors.lastname: Montoliu
authors.forename: Sebastiano
authors.initials: S
authors.lastname: Collino
authors.forename: Max
authors.initials: M
authors.lastname: Scherer
authors.forename: Philippe
authors.initials: P
authors.lastname: Guy
authors.forename: Isabelle
authors.initials: I
authors.lastname: Tavazzi
authors.forename: Anita
authors.initials: A
authors.lastname: Thorimbert
authors.forename: Sofia
authors.initials: S
authors.lastname: Moco
authors.forename: Megan P
authors.initials: MP
authors.lastname: Rothney
authors.forename: David L
authors.initials: DL
authors.lastname: Ergun
authors.forename: Maurice
authors.initials: M
authors.lastname: Beaumont
authors.forename: Fiona
authors.initials: F
authors.lastname: Ginty
authors.forename: Salah D
authors.initials: SD
authors.lastname: Qanadli
authors.forename: Lucie
authors.initials: L
authors.lastname: Favre
authors.forename: Vittorio
authors.initials: V
authors.lastname: Giusti
authors.forename: Serge
authors.initials: S
authors.lastname: Rezzi
mesh_terms: D000328:Adult; D000596:Amino Acids / Q000097:blood / Q000378:metabolism* / Q000652:urine; D050218:Body Fat Distribution; D015992:Body Mass Index; D005260:Female; D006801:Humans; D050152:Intra-Abdominal Fat / Q000378:metabolism / Q000473:pathology; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood / Q000652:urine; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000378:metabolism* / Q000473:pathology / Q000652:urine; D050151:Subcutaneous Fat / Q000378:metabolism / Q000473:pathology
chemical_list: D000596:Amino Acids; D008055:Lipids
references.citation: PLoS One. 2010 Dec 10;5(12):e15234
references.pmid: 21170321
references.citation: PLoS Genet. 2012;8(5):e1002695
references.pmid: 22589738
references.citation: Diabetologia. 2012 Oct;55(10):2622-2630
references.pmid: 22898763
references.citation: Mol Aspects Med. 2013 Feb;34(1):1-11
references.pmid: 23068073
references.citation: J Proteome Res. 2011 Dec 2;10(12):5523-35
references.pmid: 22029571
references.citation: PLoS One. 2011;6(11):e27694
references.pmid: 22096613
references.citation: Arterioscler Thromb Vasc Biol. 2005 Mar;25(3):578-84
references.pmid: 15637308
references.citation: Int J Obes (Lond). 2010 Dec;34 Suppl 2:S1-3
references.pmid: 21151141
references.citation: J Clin Invest. 2011 Apr;121(4):1402-11
references.pmid: 21403394
references.citation: J Am Coll Nutr. 2007 Aug;26(4):321-6
references.pmid: 17906183
references.citation: Diabetes Care. 2009 Sep;32(9):1678-83
references.pmid: 19502541
references.citation: Dig Dis. 2012;30(1):70-4
references.pmid: 22572689
references.citation: Appl Physiol Nutr Metab. 2007 Feb;32(1):89-114
references.pmid: 17332787
references.citation: Biochim Biophys Acta. 1995 Feb 9;1254(3):231-49
references.pmid: 7857964
references.citation: Proc Natl Acad Sci U S A. 2008 Feb 12;105(6):2117-22
references.pmid: 18252821
references.citation: J Clin Densitom. 2012 Oct-Dec;15(4):399-404
references.pmid: 22542222
references.citation: Mol Syst Biol. 2008;4:205
references.pmid: 18628745
references.citation: J Clin Endocrinol Metab. 2013 Mar;98(3):1254-63
references.pmid: 23386652
references.citation: Am J Physiol Endocrinol Metab. 2011 Jan;300(1):E175-87
references.pmid: 20978234
references.citation: Obesity (Silver Spring). 2009 Sep;17(9):1657-63
references.pmid: 19521344
references.citation: Arch Intern Med. 2008 Aug 11;168(15):1617-24
references.pmid: 18695075
references.citation: Clin Obes. 2012 Feb;2(1-2):29-40
references.pmid: 25586045
references.citation: JAMA. 2002 Oct 23-30;288(16):2008-14
references.pmid: 12387653
references.citation: Pol Arch Med Wewn. 2010;120(1-2):19-24
references.pmid: 20150840
references.citation: Anal Chem. 2005 Jan 15;77(2):517-26
references.pmid: 15649048
references.citation: Br Med J (Clin Res Ed). 1984 Nov 10;289(6454):1257-61
references.pmid: 6437507
references.citation: Curr Med Res Opin. 2007 Jan;23(1):29-47
references.pmid: 17261236
references.citation: Diabetologia. 2005 Jan;48(1):123-31
references.pmid: 15624093
references.citation: Diabetes. 2013 Feb;62(2):639-48
references.pmid: 23043162
references.citation: Mol Syst Biol. 2012;8:615
references.pmid: 23010998
references.citation: Nature. 2006 Dec 21;444(7122):1022-3
references.pmid: 17183309
references.citation: Thromb Res. 1997 May 15;86(4):337-42
references.pmid: 9187022
references.citation: Chem Phys Lipids. 2011 Sep;164(6):573-89
references.pmid: 21723266
references.citation: J Atheroscler Thromb. 2007 Feb;14(1):12-8
references.pmid: 17332687
references.citation: PLoS One. 2010 Nov 11;5(11):e13953
references.pmid: 21085649
references.citation: J Lab Clin Med. 1983 Oct;102(4):621-7
references.pmid: 6352839
references.citation: J Clin Endocrinol Metab. 2013 Apr;98(4):1541-8
references.pmid: 23450052
references.citation: Rev Med Suisse. 2007 May 16;3(111):1262-9
references.pmid: 17585631
references.citation: Biochem J. 1999 Mar 15;338 ( Pt 3):769-76
references.pmid: 10051451
references.citation: Nat Rev Microbiol. 2005 May;3(5):431-8
references.pmid: 15821725
references.citation: Diabetologia. 1994 Aug;37(8):757-64
references.pmid: 7988777
references.citation: Cell Metab. 2009 Apr;9(4):311-26
references.pmid: 19356713
references.citation: OMICS. 2012 Dec;16(12):652-67
references.pmid: 23215804
references.citation: Ann Intern Med. 1999 Jun 1;130(11):873-82
references.pmid: 10375335
references.citation: J Am Coll Cardiol. 2006 Dec 19;48(12):2531-8
references.pmid: 17174194
references.citation: Am J Physiol Endocrinol Metab. 2013 Jun 1;304(11):E1175-87
references.pmid: 23512805
references.citation: Nat Med. 2011 Apr;17(4):448-53
references.pmid: 21423183
references.citation: JAMA. 1993 Jan 27;269(4):483-7
references.pmid: 8419667
references.citation: Biochim Biophys Acta. 1985 Jan 9;833(1):170-4
references.pmid: 3967038
references.citation: Nutr J. 2011 Nov 07;10:122
references.pmid: 22059644
references.citation: PLoS Clin Trials. 2006 Jun;1(2):e12
references.pmid: 16871334
references.citation: Endocr J. 2009;56(6):773-82
references.pmid: 19506322
references.citation: PLoS One. 2007 Feb 14;2(2):e218
references.pmid: 17299598
references.citation: Lancet. 2004 Sep 11-17;364(9438):937-52
references.pmid: 15364185
references.citation: Diabetologia. 1998 Dec;41(12):1516-22
references.pmid: 9867220
references.citation: Lancet. 1987 Nov 21;2(8569):1215
references.pmid: 2890843
references.citation: Swiss Med Wkly. 2007 Mar 10;137(9-10):157-8
references.pmid: 17370157
references.citation: J Lipid Res. 2009 Apr;50 Suppl:S255-9
references.pmid: 18838739
references.citation: Prev Med. 1988 Jul;17(4):423-31
references.pmid: 3217375