title: Protection of H9c2 rat cardiomyoblasts against oxidative insults by total paeony glucosides from Radix Paeoniae Rubrae.
abstract: Total paeony glucosides (TPG) extracted from the roots of Radix Paeoniae Rubrae, have been approved for the therapy of rheumatoid arthritis by the State Food and Drug Administration. We previously demonstrated the myocardial protective effects of TPG in both isoprenaline-induced myocardial ischemia rat and acute myocardial infarction rat. However, the underlying mechanism of TPG effect in cardiomyocytes remains to be investigated. The aims of this study were to elucidate the effect of TPG on the activities of antioxidant defense targets and the bioenergetic system in rat cardiomyocytes. The changes of viability, antioxidant defense system activities, protein contents, and mitochondrial functions in tert-butyl hydroperoxide challenged H9c2 rat cardiomyoblasts were evaluated. The results suggest that TPG ameliorated cardiomyoblast dysfunction by preserving antioxidant defense and bioenergetic system.
authors.affiliation: Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology and Frontier Institute of Life Science, FIST, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Luo
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Chen
authors.forename: Yang
authors.initials: Y
authors.lastname: Cui
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Jiangang
authors.initials: J
authors.lastname: Long
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Mo
authors.forename: Jiankang
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D009202:Cardiomyopathies / Q000139:chemically induced / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005960:Glucosides / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects*; D009124:Muscle Proteins / Q000378:metabolism; D032386:Myoblasts, Cardiac / Q000187:drug effects* / Q000378:metabolism; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D029596:Paeonia / Q000737:chemistry*; D008517:Phytotherapy; D051381:Rats; D020122:tert-Butylhydroperoxide
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D009124:Muscle Proteins; D020122:tert-Butylhydroperoxide
keywords: Cell death; Mitochondria; Phase II enzymes; Reactive oxygen species; Tert-butyl hydroperoxide; Total paeony glucosides