title: Non-additive transcriptional profiles underlie dikaryotic superiority in Pleurotus ostreatus laccase activity.
abstract: BACKGROUND
  The basidiomycete Pleurotus ostreatus is an efficient producer of laccases, a group of enzymes appreciated for their use in multiple industrial processes. The aim of this study was to reveal the molecular basis of the superiority of laccase production by dikaryotic strains compared to their parental monokaryons.
  
  
  RESULTS
  We bred and studied a set of dikaryotic strains starting from a meiotic population of monokaryons. We then completely characterised the laccase allelic composition, the laccase gene expression and activity profiles in the dikaryotic strain N001, in two of its meiotic full-sib monokaryons and in the dikaryon formed from their mating.
  
  
  CONCLUSIONS
  Our results suggested that the dikaryotic superiority observed in laccase activity was due to non-additive transcriptional increases in lacc6 and lacc10 genes. Furthermore, the expression of these genes was divergent in glucose- vs. lignocellulose-supplemented media and was highly correlated to the detected extracellular laccase activity. Moreover, the expression profile of lacc2 in the dikaryotic strains was affected by its allelic composition, indicating a putative single locus heterozygous advantage.
authors.affiliation: Genetics and Microbiology Research Group, Department of Agrarian Production, Public University of Navarre, Pamplona, Navarre, Spain.
authors.forename: Raúl
authors.initials: R
authors.lastname: Castanera
authors.forename: Alejandra
authors.initials: A
authors.lastname: Omarini
authors.forename: Francisco
authors.initials: F
authors.lastname: Santoyo
authors.forename: Gúmer
authors.initials: G
authors.lastname: Pérez
authors.forename: Antonio G
authors.initials: AG
authors.lastname: Pisabarro
authors.forename: Lucía
authors.initials: L
authors.lastname: Ramírez
mesh_terms: D015870:Gene Expression; D020869:Gene Expression Profiling; D005800:Genes, Fungal; D042845:Laccase / Q000235:genetics* / Q000378:metabolism; D020076:Pleurotus / Q000201:enzymology* / Q000235:genetics
chemical_list: D042845:Laccase
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