title: Adeno-associated virus (AAV) vectors in gene therapy: immune challenges and strategies to circumvent them.
abstract: AAV-based gene transfer protocols have shown remarkable success when directed to immune-privileged sites such as for retinal disorders like Lebers congenital amaurosis. In contrast, AAV-mediated gene transfer into liver or muscle tissue for diseases such as hemophilia B, α1 anti-trypsin deficiency and muscular dystrophy has demonstrated a decline in gene transfer efficacy over time. It is now known that in humans, AAV triggers specific pathways that recruit immune sensors. These factors initiate an immediate reaction against either the viral capsid or the vector encoded protein as part of innate immune response or to produce a more specific adaptive response that generates immunological memory. The vector-transduced cells are then rapidly destroyed due to this immune activation. However, unlike other viral vectors, AAV is not immunogenic in murine models. Its immunogenicity becomes apparent only in large animal models and human subjects. Moreover, humans are natural hosts to AAV and exhibit a high seroprevalence against AAV vectors. This limits the widespread application of AAV vectors into patients with pre-existing neutralising antibodies or memory T cells. To address these issues, various strategies are being tested. Alternate serotype vectors (AAV1-10), efficient expression cassettes, specific tissue targeting, immune-suppression and engineered capsid variants are some approaches proposed to minimise this immune stimulation. In this review, we have summarised the nature of the immune response documented against AAV in various pre-clinical and clinical settings and have further discussed the strategies to evade them.
authors.affiliation: Centre for Stem Cell Research, Christian Medical College, Vellore, Tamil Nadu, India.
authors.forename: Sangeetha
authors.initials: S
authors.lastname: Hareendran
authors.forename: Balaji
authors.initials: B
authors.lastname: Balakrishnan
authors.forename: Dwaipayan
authors.initials: D
authors.lastname: Sen
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kumar
authors.forename: Alok
authors.initials: A
authors.lastname: Srivastava
authors.forename: Giridhara R
authors.initials: GR
authors.lastname: Jayandharan
mesh_terms: D056704:Adaptive Immunity; D000257:Adenoviridae Infections / Q000276:immunology; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000229:Dependovirus / Q000235:genetics / Q000276:immunology*; D004337:Drug Carriers; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate; D016036:Seroepidemiologic Studies
chemical_list: D000914:Antibodies, Viral; D004337:Drug Carriers