title: Evaluation of therapeutic potential of nanosilver particles synthesised using aloin in experimental murine mastitis model.
abstract: Nanobiotechnology is an emerging biological branch of nanotechnology. Application of nanoparticles with specific size and shape in biology has already shown unforeseen and interesting results. A study was conducted to evaluate the therapeutic potential of phytogenically derived aloin mediated nanosilver particles (AAgNPs), prepared by reduction of silver nitrate with aloin, in Staphylococcus aureus induced murine mastitis. A total of 40 female mice were divided into five groups of eight animals each. Group I served as lactating control, groups II-V were inoculated with 20 μl of 24 h broth culture of S. aureus containing 4.0 × 105 cfu/quarter under ketamine anaesthesia. After 6 h post inoculation, groups III and IV received 20 μl of aloin nanosilver (AAgNPs) through intramammary and intraperitoneal routes, respectively. Group V received antibiotic cefepime at 1 mg/kg body weight through the intra-peritoneal route. After 18 h post-treatment, serum C reactive protein, weights of mammary glands, mammary gland bacterial load, thiobarbituric acid reactive substances content, reduced glutathione content, superoxide dismutase activity and catalase activity and histopathology were determined. The compound showed a minimum inhibitory concentration of 21.8 ng/ml against S. aureus. Significant reduction (98%) in poly-morpho nuclear cell infiltration was observed with AAgNPs than antibiotic (50%).
authors.forename: Thota Venkata
authors.initials: TV
authors.lastname: Chaitanya Kumar
authors.forename: Yegireddy
authors.initials: Y
authors.lastname: Muralidhar
authors.forename: Pagadala Eswara
authors.initials: PE
authors.lastname: Prasad
authors.forename: Tollamadugu Naga Venkata Krishna Vara
authors.initials: TN
authors.lastname: Prasad
authors.forename: Mekapogu
authors.initials: M
authors.lastname: Alpha Raj
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001835:Body Weight; D004195:Disease Models, Animal; D015195:Drug Design; D004642:Emodin / Q000031:analogs & derivatives* / Q000627:therapeutic use; D005260:Female; D008413:Mastitis / Q000188:drug therapy* / Q000382:microbiology; D053768:Metal Nanoparticles / Q000627:therapeutic use*; D051379:Mice; D010802:Phylogeny; D012834:Silver / Q000627:therapeutic use*; D013203:Staphylococcal Infections / Q000188:drug therapy*
chemical_list: D000975:Antioxidants; D012834:Silver; D004642:Emodin; C006457:alloin