title: [Pulsed-field gel electrophoresis typing on non-O157 Shiga toxin-producing Escherichia coli isolates].
abstract: OBJECTIVE
  To establish a database and to understand the molecular epidemiological features of non-O157 Shiga toxin-producing Escherichia coli (STEC) isolates from different animal reservoirs and patients.
  
  
  METHODS
  Pulsed-field gel electrophoresis (PFGE) was performed according to the PulseNet protocol with minor modifications. A dendrogram was constructed using the BioNumerics.
  
  
  RESULTS
  Under the PulseNet protocol, 62 PFGE patterns were obtained from 76 non-O157 STEC isolates and then divided into A to M groups. Isolates from different sources were widely distributed in different groups, but were predominant seen in certain groups.
  
  
  CONCLUSIONS
  The non-O157 STEC isolates in China were highly polymorphic. PulseNet protocol seemed to be suitable for the typing of Chinese non-O157 STEC isolates.
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Jin
authors.forename: Ai-lan
authors.initials: AL
authors.lastname: Zhao
authors.forename: Xiang-ning
authors.initials: XN
authors.lastname: Bai
authors.forename: Qiong
authors.initials: Q
authors.lastname: Meng
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.forename: Xue-jiao
authors.initials: XJ
authors.lastname: Yuan
authors.forename: Yan-wen
authors.initials: YW
authors.lastname: Xiong
authors.forename: Xue-xin
authors.initials: XX
authors.lastname: Hou
authors.forename: Zhen-jun
authors.initials: ZJ
authors.lastname: Li
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D004269:DNA, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D005838:Genotype; D006801:Humans; D054323:Shiga-Toxigenic Escherichia coli
chemical_list: D004269:DNA, Bacterial