title: [Study on PrP106-126 peptide disturbed dimeration of 14-3-3beta].
abstract: OBJECTIVE
  To investigate both PrP and PrP106-126 peptide effect on 14-3-3beta dimeration.
  
  
  METHODS
  14-3-3beta were incubated with different does recombinant PrP or PrP106-126 peptide, both 14-3-3beta dimer and polymer were separated 15% non-denaturing polyacrylamide gel electrophoresis (PAGE) and the 14-3-3 dimers were evaluated using 14-3-3beta-specific Western blotting. And then,14-3-3beta dimeration buffer were incubated with different does recombinant PrP and 250 micromol/L PrP106-126 peptide, 14-3-3beta dimer and polymer were detected by above methods. Cellular 14-3-3 dimer were also detected after PrP106-126 peptide were added to HeLa cell for 8 hours.
  
  
  RESULTS
  Recombinant full-length PrP facilitated the dimerization of 14-3-3beta and PrP106-126 disturbed 14-3-3beta dimeration as both have dose dependence effect. PrP antagonized PrP106-126-induced 14-3-3beta dimer with PrP protein increase in vitro. Cellular 14-3-3 dimerization also decreased after treatment of peptide PrP106-126 on HeLa cells for 8 hours.
  
  
  CONCLUSIONS
  [corrected] Dimerization process of 14-3-3beta was promoted by full-length PrP (PrP23-231) but inhibited by peptide PrP106-126 in vitro. PrP agonized PrP106-126-induced inhibition of 14-3-3 dimeration. PrP106-126 inhibited cellular 14-3-3 dimerization.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Qin-Qin
authors.initials: QQ
authors.lastname: Song
authors.forename: Peng
authors.initials: P
authors.lastname: Sun
authors.forename: Juan
authors.initials: J
authors.lastname: Song
authors.forename: Lin-Jun
authors.initials: LJ
authors.lastname: Sheng
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Han
mesh_terms: D048948:14-3-3 Proteins / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D010446:Peptide Fragments / Q000494:pharmacology*; D011328:Prions / Q000494:pharmacology*; D055503:Protein Multimerization / Q000187:drug effects*; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D048948:14-3-3 Proteins; D010446:Peptide Fragments; D011328:Prions; D011994:Recombinant Proteins; C082632:prion protein (106-126)