title: DEAD box protein DDX1 regulates cytoplasmic localization of KSRP.
abstract: mRNA decay mediated by the AU-rich elements (AREs) is one of the most studied post-transcriptional mechanisms and is modulated by ARE-binding proteins (ARE-BPs). To understand the regulation of K homology splicing regulatory protein (KSRP), a decay-promoting ARE-BP, we purified KSRP protein complexes and identified an RNA helicase, DDX1. We showed that down-regulation of DDX1 expression elevated cytoplasmic levels of KSRP and facilitated ARE-mediated mRNA decay. Association of KSRP with 14-3-3 proteins, that are predominately located in the cytoplasm, increased upon reduction of DDX1. We also demonstrated that KSRP associated with DDX1 or 14-3-3, but not both. These observations indicate that subcellular localization of KSRP is regulated by competing interactions with DDX1 or 14-3-3.
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama, United States of America.
authors.forename: Chu-Fang
authors.initials: CF
authors.lastname: Chou
authors.forename: Wei-Jye
authors.initials: WJ
authors.lastname: Lin
authors.forename: Chen-Chung
authors.initials: CC
authors.lastname: Lin
authors.forename: Christian A
authors.initials: CA
authors.lastname: Luber
authors.forename: Roseline
authors.initials: R
authors.lastname: Godbout
authors.forename: Matthias
authors.initials: M
authors.lastname: Mann
authors.forename: Ching-Yi
authors.initials: CY
authors.lastname: Chen
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism; D021581:Active Transport, Cell Nucleus; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism*; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D016601:RNA-Binding Proteins / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism*
chemical_list: D048948:14-3-3 Proteins; C105789:KHSRP protein, human; D016601:RNA-Binding Proteins; D015534:Trans-Activators; C086252:DDX1 protein, human; D053487:DEAD-box RNA Helicases
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