title: Differential cellular gene expression in duck trachea infected with a highly or low pathogenic H5N1 avian influenza virus.
abstract: BACKGROUND
  Avian influenza A (AI) viruses of subtypes H5 can cause serious disease outbreaks in poultry including panzootic due to H5N1 highly pathogenic (HP) viruses. These viruses are a threat not only for animal health but also public health due to their zoonotic potential. The domestic duck plays a major role in the epidemiological cycle of influenza virus subtypes H5 but little is known concerning host/pathogen interactions during influenza infection in duck species. In this study, a subtracted library from duck trachea (a primary site of influenza virus infection) was constructed to analyse and compare the host response after a highly or low pathogenic (LP) H5N1-infection.
  
  
  RESULTS
  Here, we show that more than 200 different genes were differentially expressed in infected duck trachea to a significant degree. In addition, significant differentially expressed genes between LPAI- and HPAI-infected tracheas were observed. Gene ontology annotation was used and specific signalling pathways were identified. These pathways were different for LPAI and HPAI-infected tracheas, except for the CXCR4 signalling pathway which is implicated in immune response. A different modulation of genes in the CXCR4 signalling pathway and TRIM33 was induced in duck tracheas infected with a HPAI- or a LPAI-H5N1.
  
  
  CONCLUSIONS
  First, this study indicates that Suppressive Subtractive Hybridization (SSH) is an alternative approach to gain insights into the pathogenesis of influenza infection in ducks. Secondly, the results indicate that cellular gene expression in the duck trachea was differently modulated after infection with a LPAI-H5N1 or after infection with a HPAI-H5N1 virus. Such difference found in infected trachea, a primary infection site, could precede continuation of infection and could explain appearance of respiratory symptoms or not.
authors.affiliation: Anses Ploufragan/Plouzané laboratory, Virologie Immunologie Parasitologie Aviaires et Cunicoles, B,P, 53, Ploufragan 22440, France. pascale.massin@anses.fr.
authors.forename: Pascale
authors.initials: P
authors.lastname: Massin
authors.forename: Claire
authors.initials: C
authors.lastname: Deleage
authors.forename: Aurélie
authors.initials: A
authors.lastname: Oger
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Briand
authors.forename: Hélène
authors.initials: H
authors.lastname: Quenault
authors.forename: Yannick
authors.initials: Y
authors.lastname: Blanchard
mesh_terms: D000818:Animals; D004372:Ducks; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D053124:Influenza A Virus, H5N1 Subtype / Q000472:pathogenicity*; D005585:Influenza in Birds / Q000473:pathology* / Q000821:virology*; D009693:Nucleic Acid Hybridization / Q000379:methods; D015398:Signal Transduction / Q000235:genetics; D014132:Trachea / Q000473:pathology* / Q000821:virology*
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