title: A unique dermal dendritic cell subset that skews the immune response toward Th2.
abstract: Dendritic cell (DC) subsets in the skin and draining lymph nodes (LNs) are likely to elicit distinct immune response types. In skin and skin-draining LNs, a dermal DC subset expressing macrophage galactose-type C-type lectin 2 (MGL2/CD301b) was found distinct from migratory Langerhans cells (LCs) or CD103(+) dermal DCs (dDCs). Lower expression levels of Th1-promoting and/or cross-presentation-related molecules were suggested by the transcriptome analysis and verified by the quantitative real-time PCR analysis in MGL2(+) dDCs than in CD103(+) dDCs. Transfer of MGL2(+) dDCs but not CD103(+) dDCs from FITC-sensitized mice induced a Th2-type immune response in vivo in a model of contact hypersensitivity. Targeting MGL2(+) dDCs with a rat monoclonal antibody against MGL2 efficiently induced a humoral immune response with Th2-type properties, as determined by the antibody subclass. We propose that the properties of MGL2(+) dDCs, are complementary to those of CD103(+) dDCs and skew the immune response toward a Th2-type response.
authors.affiliation: Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, the University of Tokyo, Tokyo, Japan.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Murakami
authors.forename: Kaori
authors.initials: K
authors.lastname: Denda-Nagai
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Hashimoto
authors.forename: Shigenori
authors.initials: S
authors.lastname: Nagai
authors.forename: Masahira
authors.initials: M
authors.lastname: Hattori
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Irimura
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D003877:Dermatitis, Contact / Q000276:immunology; D005260:Female; D039001:Integrin alpha Chains / Q000276:immunology; D037181:Lectins, C-Type / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D051381:Rats; D012867:Skin / Q000166:cytology / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D039001:Integrin alpha Chains; D037181:Lectins, C-Type; C464940:MGL2 protein, mouse; C091367:alpha E integrins
references.citation: J Exp Med. 2008 Jan 21;205(1):13-7
references.pmid: 18195077
references.citation: Proc Natl Acad Sci U S A. 2011 Feb 8;108(6):2384-9
references.pmid: 21262813
references.citation: Semin Immunol. 2001 Oct;13(5):275-82
references.pmid: 11502162
references.citation: Immunity. 2012 Apr 20;36(4):646-57
references.pmid: 22483802
references.citation: J Clin Invest. 2012 May;122(5):1615-27
references.pmid: 22505458
references.citation: Nature. 2006 May 11;441(7090):235-8
references.pmid: 16648838
references.citation: Nat Rev Immunol. 2012 Jan 13;12(2):114-24
references.pmid: 22240625
references.citation: J Leukoc Biol. 2012 Mar;91(3):437-48
references.pmid: 22147811
references.citation: J Invest Dermatol. 2010 Dec;130(12):2752-9
references.pmid: 20703247
references.citation: Immunity. 2004 Jul;21(1):107-19
references.pmid: 15345224
references.citation: Nature. 2011 Sep 14;477(7366):596-600
references.pmid: 21918512
references.citation: J Exp Med. 1998 Jul 20;188(2):297-304
references.pmid: 9670042
references.citation: J Leukoc Biol. 2005 Sep;78(3):656-64
references.pmid: 15961574
references.citation: Nature. 1998 Mar 19;392(6673):245-52
references.pmid: 9521319
references.citation: Blood. 2002 Sep 1;100(5):1734-41
references.pmid: 12176895
references.citation: Science. 2007 Jan 5;315(5808):107-11
references.pmid: 17204652
references.citation: J Biochem. 1992 Mar;111(3):331-6
references.pmid: 1587794
references.citation: Immunity. 2009 Nov 20;31(5):823-33
references.pmid: 19913446
references.citation: Annu Rev Immunol. 2004;22:891-928
references.pmid: 15032599
references.citation: J Immunol. 2011 Feb 15;186(4):2422-9
references.pmid: 21242525
references.citation: Nature. 2011 Aug 28;477(7366):592-5
references.pmid: 21874021
references.citation: J Immunol. 2007 Nov 15;179(10):6830-5
references.pmid: 17982073
references.citation: J Exp Med. 2007 Dec 24;204(13):3147-56
references.pmid: 18086865
references.citation: J Clin Invest. 2012 May;122(5):1628-43
references.pmid: 22505455
references.citation: Immunity. 2006 Jul;25(1):153-62
references.pmid: 16860764
references.citation: Int Immunol. 2004 May;16(5):685-95
references.pmid: 15096484
references.citation: J Immunol. 2010 Mar 15;184(6):2974-84
references.pmid: 20173025
references.citation: J Exp Med. 1987 Dec 1;166(6):1654-67
references.pmid: 3119761
references.citation: J Allergy Clin Immunol. 2010 May;125(5):1154-1156.e2
references.pmid: 20226508
references.citation: Nat Immunol. 2009 Jul;10(7):706-12
references.pmid: 19465908
references.citation: Annu Rev Immunol. 1995;13:251-76
references.pmid: 7612223
references.citation: Blood. 2011 Sep 1;118(9):2502-10
references.pmid: 21750314
references.citation: Nat Rev Immunol. 2002 Feb;2(2):116-26
references.pmid: 11910893
references.citation: Immunol Cell Biol. 2010 May-Jun;88(4):366-75
references.pmid: 20231850
references.citation: Cytokine Growth Factor Rev. 2003 Oct;14(5):381-9
references.pmid: 12948522
references.citation: PLoS One. 2009;4(5):e5619
references.pmid: 19440334
references.citation: Science. 2008 Nov 14;322(5904):1097-100
references.pmid: 19008445
references.citation: J Immunol. 1980 Jan;124(1):445-53
references.pmid: 6153101
references.citation: J Immunol. 2009 Sep 1;183(5):2995-3003
references.pmid: 19667086
references.citation: Mol Immunol. 2008 Sep;45(15):4020-7
references.pmid: 18657861
references.citation: J Immunol. 2005 Apr 15;174(8):5082-91
references.pmid: 15814739
references.citation: Blood. 2010 Mar 11;115(10):1958-68
references.pmid: 20068222
references.citation: J Immunol. 1996 Jan 1;156(1):128-35
references.pmid: 8598452
references.citation: J Immunol. 2000 Dec 15;165(12):6783-90
references.pmid: 11120799
references.citation: Annu Rev Immunol. 1996;14:233-58
references.pmid: 8717514
references.citation: J Immunol. 1990 Nov 1;145(9):2833-8
references.pmid: 2212665
references.citation: Immunity. 2005 Dec;23(6):611-20
references.pmid: 16356859
references.citation: J Immunol. 2011 Nov 15;187(10):5069-76
references.pmid: 21998450
references.citation: Proc Natl Acad Sci U S A. 1989 Jan;86(2):612-6
references.pmid: 2643119
references.citation: Nat Immunol. 2001 Dec;2(12):1144-50
references.pmid: 11713464
references.citation: J Exp Med. 2007 Dec 24;204(13):3119-31
references.pmid: 18086861
references.citation: Int Immunol. 2011 Aug;23(8):467-72
references.pmid: 21677049
references.citation: J Exp Med. 2000 Jan 17;191(2):375-80
references.pmid: 10637281
references.citation: Nat Immunol. 2009 Dec;10(12):1237-44
references.pmid: 19915624
references.citation: J Exp Med. 2010 May 10;207(5):953-61
references.pmid: 20421390
references.citation: Nature. 2005 Feb 24;433(7028):887-92
references.pmid: 15711573
references.citation: Blood. 2012 Mar 8;119(10):2284-92
references.pmid: 22234694
references.citation: Nat Rev Immunol. 2007 Jan;7(1):19-30
references.pmid: 17170756
references.citation: J Immunol. 2007 Apr 15;178(8):5296-304
references.pmid: 17404314
references.citation: J Biol Chem. 2010 Jun 18;285(25):19193-204
references.pmid: 20304916
references.citation: J Exp Med. 2011 Aug 29;208(9):1789-97
references.pmid: 21859845
references.citation: J Biol Chem. 1996 Aug 23;271(34):20545-50
references.pmid: 8702798
references.citation: Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3312-7
references.pmid: 19218433
references.citation: Immunity. 2010 May 28;32(5):681-91
references.pmid: 20493731
references.citation: Annu Rev Immunol. 2001;19:423-74
references.pmid: 11244043
references.citation: Nat Immunol. 2009 Jul;10(7):697-705
references.pmid: 19465906
references.citation: J Cell Biol. 2005 May 23;169(4):569-76
references.pmid: 15897263
references.citation: Immunity. 2011 Aug 26;35(2):260-72
references.pmid: 21782478
references.citation: Eur J Immunol. 2010 Aug;40(8):2089-94
references.pmid: 20853491
references.citation: Immunity. 2005 May;22(5):643-54
references.pmid: 15894281
references.citation: J Invest Dermatol. 2003 Feb;120(2):266-74
references.pmid: 12542532
references.citation: PLoS One. 2011;6(10):e26919
references.pmid: 22066016
references.citation: Annu Rev Immunol. 2012;30:1-22
references.pmid: 22136168
references.citation: Nat Immunol. 2000 Sep;1(3):199-205
references.pmid: 10973276
references.citation: J Exp Med. 2010 Jan 18;207(1):189-206
references.pmid: 20038600
references.citation: J Biol Chem. 2002 Aug 9;277(32):28892-901
references.pmid: 12016228
references.citation: J Clin Invest. 2003 Oct;112(7):1058-66
references.pmid: 14523043
references.citation: Cell. 2004 May 14;117(4):515-26
references.pmid: 15137944
references.citation: J Exp Med. 2007 Dec 24;204(13):3133-46
references.pmid: 18086862
references.citation: Vaccine. 2011 Jan 29;29(5):984-93
references.pmid: 21115055