title: Characterization of leptospira borgpetersenii isolates from field rats (rattus norvegicus) by 16s rrna and lipl32 gene sequencing.
abstract: The main goal of this study was to evaluate the prevalence of leptospirosis among field rodents of Tiruchirappalli district, Tamil Nadu, India. In total 35 field rats were trapped and tested for seroprevalence by the microscopic agglutination test (MAT). Isolation of leptospires was performed from blood and kidney tissues and characterized to serovar level. Genomospecies identification was carried out using 16S rRNA and lipL32 gene sequencing. The molecular phylogeny was constructed to find out species segregation. Seroprevalence was about 51.4 %, and the predominant serovars were Autumnalis, Javanica, Icterohaemorrhagiae and Pomona. Two isolates from the kidneys were identified as serovar Javanica of Serogroup Javanica, and sequence based molecular phylogeny indicated these two isolates were Leptospira borgpetersenii.
authors.affiliation: Division of Medical Microbiology, Department of Microbiology, School of Life Sciences, Bharathidasan University , Tiruchirappalli-620024 , Tamilnadu, India.
authors.forename: Kumaresan
authors.initials: K
authors.lastname: Vedhagiri
authors.forename: Kalimuthusamy
authors.initials: K
authors.lastname: Natarajaseenivasan
authors.forename: Shanmugarajan G
authors.initials: SG
authors.lastname: Prabhakaran
authors.forename: Joseph
authors.initials: J
authors.lastname: Selvin
authors.forename: Ramasamy
authors.initials: R
authors.lastname: Narayanan
authors.forename: Yogesh S
authors.initials: YS
authors.lastname: Shouche
authors.forename: Paluru
authors.initials: P
authors.lastname: Vijayachari
authors.forename: Sivalingam
authors.initials: S
authors.lastname: Ratnam
keywords: 16S rRNA; Leptospira borgpetersenii; Leptospirosis; lipL32
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