title: Identical pathogenesis and neuropathological phenotype of scrapie in valine, arginine, glutamine/valine, arginine, glutamine sheep infected experimentally by the oral and conjunctival routes.
abstract: The pathogenesis of scrapie in sheep after natural or oral exposure to the infectious agent generally involves the early accumulation of disease-associated prion protein (PrP(d)) in the lymphoreticular system (LRS). This phase is followed by neuroinvasion, for which two routes, ascending neural and haematogenous, have been postulated. The present study reports the use of immunohistochemistry to track the tissue progression of PrP(d) deposition in sheep of a single, highly scrapie-susceptible PrP genotype administered by the oral or conjunctival routes. Regardless of the route of infection, the earliest detection of PrP(d) was in gut- and pharynx-associated LRS tissues. Subsequently, the brain became PrP(d) positive simultaneously with other LRS tissues, but before the spinal cord and peripheral nervous tissues of the enteric, parasympathetic and sympathetic systems. The sites of initial PrP(d) accumulation in the brain were the dorsal motor nucleus of the vagus and the hypothalamus and their related circumventricular organs (the area postrema and the median eminence, respectively). These were the same for both routes of infection. Rapid progression to clinical disease was observed in sheep infected orally or conjunctivally, with definite signs of scrapie recorded at around 6 and 8 months after infection, respectively. Longer incubation periods in sheep infected by the conjunctival route were probably due to them receiving a lower dose than those infected orally. Irrespective of the route of infection, clinically affected sheep showed the same pathological phenotype (PrP(d) profile) and PrP(d) distribution throughout the brain. The identical peripheral and central pathogenesis observed in sheep of both groups suggests early dissemination of the infectious agent in the bloodstream and a common neuroinvasion pathway. The late involvement of the enteric and autonomic nervous system supports a haematogenous route of infection to the brain.
authors.affiliation: Animal Health and Veterinary Laboratories Agency (AHVLA), Penicuik, Midlothian EH26 0PZ, UK. Electronic address: lorenzo.gonzalez@ahvla.gsi.gov.uk.
authors.forename: L
authors.initials: L
authors.lastname: González
authors.affiliation: Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza 50013, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Pitarch
authors.affiliation: Animal Health and Veterinary Laboratories Agency (AHVLA), Penicuik, Midlothian EH26 0PZ, UK.
authors.forename: S
authors.initials: S
authors.lastname: Martin
authors.affiliation: Animal Health and Veterinary Laboratories Agency (AHVLA), Addlestone, Surrey KT15 3NB, UK.
authors.forename: L
authors.initials: L
authors.lastname: Thurston
authors.affiliation: Animal Health and Veterinary Laboratories Agency (AHVLA), Addlestone, Surrey KT15 3NB, UK.
authors.forename: J
authors.initials: J
authors.lastname: Moore
authors.affiliation: Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza 50013, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Acín
authors.affiliation: Animal Health and Veterinary Laboratories Agency (AHVLA), Penicuik, Midlothian EH26 0PZ, UK.
authors.forename: M
authors.initials: M
authors.lastname: Jeffrey
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology*; D018450:Disease Progression; D005838:Genotype; D018620:PrPSc Proteins / Q000235:genetics* / Q000378:metabolism; D012608:Scrapie / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D012756:Sheep; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology*
chemical_list: D018620:PrPSc Proteins
keywords: pathogenesis; prion protein; scrapie; sheep