title: Preparation and immunoprotection of subgroup B avian leukosis virus inactivated vaccine.
abstract: OBJECTIVE
  To develop an inactivated vaccine against subgroup B avian leukosis virus (ALV-B) and determine if vaccination of chicken breeders could protect young chicks from ALV-B horizontal infection at early stage and accelerate eradication progress.
  
  
  METHODS
  Chicken embryo fibroblast (CEF) cells were inoculated with SDAU09C2 strain of ALV-B and ALV-CEF was inactivated for preparation of oil-adjuvant vaccine. Eggs were collected from un-vaccinated and 9 vaccinated great parent female chickens for incubation. 1-day-old chicks were bled for testing their maternal antibodies to ALV-A/B and then inoculated with ALV-B. Viremia and cloaca p27 detection dynamics were tested and compared between chick groups with or without maternal antibody to ALV.
  
  
  RESULTS
  In 3 weeks after 3 vaccination with the inactivated vaccine, all 9 vaccinated breeders developed high antibody titers against ALV-A/B with ELISA read values of 1.69-1.89 (the positive base line was 0.4) and kept at the high titers for at least another 4 weeks. Maternal antibody was detected in 70% (12/17) of chicks from breeders with high antibody titers to ALV-A/B. Only 4 of 12 chickens with maternal antibodies developed temporary viremia and no viremia was detected in the left 8 maternal antibody positive chickens during the whole 14 week after inoculation of ALV-B at 1 day of age. But the persistent viremia was detected in 2-8 weeks in all 9 maternal antibody negative chickens and the viremia persisted in the whole tested period of 14 weeks after inoculation of ALV-B.
  
  
  CONCLUSIONS
  The inactivated ALV-B vaccine could induce high titer antibody reaction to ALV-B, it could provide maternal antibodies to 1-day-old chickens and protect chickens from early infection of ALV-B.
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Shandong Engineering Research Center for Animal Disease Control and Prevention, Taian 271018, China. Electronic address: lixue880209@163.com.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.forename: Xuan
authors.initials: X
authors.lastname: Dong
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Sun
authors.forename: Weihua
authors.initials: W
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Cui
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001353:Avian Leukosis / Q000517:prevention & control*; D001354:Avian Leukosis Virus / Q000276:immunology*; D002645:Chickens; D002988:Cloaca / Q000821:virology; D005260:Female; D007112:Immunity, Maternally-Acquired; D011201:Poultry Diseases / Q000517:prevention & control*; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D014766:Viremia / Q000517:prevention & control
chemical_list: D000914:Antibodies, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines
keywords: Avian leukosis virus subgroup B; Immune protection; Inactivated vaccine; Maternal antibodies; p27