title: Relationship between γ-interferon gene polymorphisms and susceptibility to brucellosis infection.
abstract: Interferon-gamma (IFN-γ) is a pro-inflammatory cytokine that plays a pivotal role in the defense mechanism against Brucella infection. It was hypothesized that the IFN-γ in (+874 A/T in intron 1) TT and +5644 T/A, TT genotypes, which are reportedly associated with high IFN production, are associated with susceptibility to brucellosis in Iranian subjects. Genotyping of these IFN-γ variants by an allele-specific polymerase chain reaction method was performed in 281 subjects, comprising 153 patients with active brucellosis and 128 healthy controls. It was found that the +874 minor allele (A) and homozygote genotype (AA) were significantly more frequently present in brucellosis patients than in controls (OR = 2.588; 95% CI, 1.313-5.104; P = 0.006 for the AA genotype; OR = 1.575; 95% CI, 1.124-2.216; P = 0.010 for the A allele). However, the allelic and genotypic distribution of the IFN-γ polymorphism at position UTR5644 A>T did not differ significantly between patients and controls (P > 0.05). The distribution of haplotypes in this study suggests that the T/A haplotype (+874/UTR5644), which was present more frequently in controls than in patients, may protect subjects against Brucella infection. It is suggested that IFN-γ +874 AA genotype and A allele are risk factors for developing brucellosis infection in Iranian subjects.
authors.affiliation: Cellular and Molecular Research Center; Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Khalije Fars Boulevarde, Zahedan 14155‐6455,.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Eskandari-Nasab
authors.forename: Mehdi
authors.initials: M
authors.lastname: Moghadampour
authors.forename: Seyed-Shahaboddin
authors.initials: SS
authors.lastname: Hasani
authors.forename: Mehdi
authors.initials: M
authors.lastname: Hadadi-fishani
authors.forename: Seyyed-Ali
authors.initials: SA
authors.lastname: Mirghanizadeh-Bafghi
authors.forename: Abolghasem
authors.initials: A
authors.lastname: Asadi-Saghandi
authors.forename: Fateme
authors.initials: F
authors.lastname: Zare
authors.forename: Behrooz
authors.initials: B
authors.lastname: Sadeghi-Kalani
authors.forename: Mehran
authors.initials: M
authors.lastname: Ghazali-bina
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002002:Brucella / Q000502:physiology*; D002006:Brucellosis / Q000235:genetics* / Q000382:microbiology*; D016022:Case-Control Studies; D002648:Child; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D007492:Iran; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D044465:White People / Q000235:genetics; D055815:Young Adult
chemical_list: D007371:Interferon-gamma
keywords: Brucellosis; gene polymorphism; interferon-gamma