title: [Inhibitory effects of human umbilical cord-derived mesenchymal stem cells on proliferation of peripheral blood mononuclear cells from spondyloarthritis patients].
abstract: OBJECTIVE
  To explore the inhibitory effects of human umbilical cord-derived mesenchymal stem cells (hUCMSC) on the proliferation of peripheral blood mononuclear cells (PBMC) from spondyloarthritis (SpA) patients.
  
  
  METHODS
  A total of 12 SpA patients at Chinese PLA General Hospital were recruited from May 2012 to October 2012. Information on demographic characteristics, disease and functional activity was collected. Isolated PBMC were stimulated by phytohemagglutinin (PHA, 1 µg/ml) in the presence or absence of hUCMSC.The proliferation of hUCMSC was suppressed by irradiation with Co60 (30 Gy) before co-culturing with PBMC. The proliferation of PBMC was determined by Cell Counting Kit-8 (CCK-8). Cell cycle profiles of PBMC were analyzed by flow cytometry. The association of inhibitory effect of hUCMSC with the disease and functional activity of SpA patients was examined.
  
  
  RESULTS
  After coculturing with hUCMSC by cell-to-cell contact for 5 days, the proliferation of PBMC stimulated by PHA (1 µg/ml) was significantly inhibited by hUCMSC in a dose-dependent manner.The inhibition rate of the proliferation of PBMC cocultured with hUCMSC by cell-to-cell contact was higher than that by Transwell culture (57% ± 17% vs 32% ± 12%, P < 0.01). Compared to PBMC cultured alone, a larger number of PBMC cocultured with hUCMSC were in phase G1 (86% ± 3% vs 68% ± 5%, P < 0.01) while a lower number of cells in phases S and G2 (8% ± 3% vs 26% ± 5%, P < 0.01). No association was found between the inhibitory effect of hUCMSC and the disease and functional activity.
  
  
  CONCLUSIONS
  The proliferation of PBMC from SpA patients may be inhibited by hUCMSC. And hUCMSC have therapeutic potentials for SpA patients.
authors.affiliation: Department of Rheumatology, Chinese PLA General Hospital, Beijing, China. fhuang1964@yahoo.com
authors.forename: Zhi-fang
authors.initials: ZF
authors.lastname: Huang
authors.forename: Shuang-hong
authors.initials: SH
authors.lastname: Lü
authors.forename: Jian
authors.initials: J
authors.lastname: Zhu
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Yang
authors.forename: Ya-kun
authors.initials: YK
authors.lastname: Song
authors.forename: Li-xin
authors.initials: LX
authors.lastname: DU
authors.forename: Xian-da
authors.initials: XD
authors.lastname: Chen
authors.forename: Hai-xu
authors.initials: HX
authors.lastname: Hu
authors.forename: Dong-ying
authors.initials: DY
authors.lastname: Wu
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Huang
mesh_terms: D000328:Adult; D002453:Cell Cycle; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D025241:Spondylarthritis / Q000473:pathology*; D014470:Umbilical Cord / Q000166:cytology