title: Comparison of three decontamination methods for Mycobacterium bovis isolation.
abstract: SIXTY SAMPLES OF TISSUE FRAGMENTS WITH LESIONS SUGGESTIVE OF TUBERCULOSIS FROM BOVINE ABATTOIRS, KEPT IN SATURATED SOLUTION OF SODIUM BORATE, WERE SUBJECTED TO FOUR TREATMENTS: 4% NaOH (Petroff Method), 12 % H2SO4 and 1.5% HPC (1-Hexadecylpyridinium Chloride) decontamination, and physiological saline solution (control). The HPC method showed the lowest contamination rate (3%) when compared to control (88%, p<0.001), NaOH (33%, p<0.001) and H2SO4 (21.7%, p<0.002). Regarding the isolation success, the HPC method was better (40%) than the control (3%, p<0.001), NaOH (13%, p=0.001) and H2SO4 (1.7%, p<0.001) methods. These results indicate that HPC is an alternative to the Petroff method.
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidãde de São Paulo , São Paulo, SP , Brasil.
authors.forename: Simone Rodrigues
authors.initials: SR
authors.lastname: Ambrosio
authors.forename: Eugenia Márcia
authors.initials: EM
authors.lastname: de Deus Oliveira
authors.forename: Cesar Alejandro Rosales
authors.initials: CA
authors.lastname: Rodriguez
authors.forename: José Soares
authors.initials: JS
authors.lastname: Ferreira Neto
authors.forename: Marcos
authors.initials: M
authors.lastname: Amaku
keywords: HPC; Mycobacterium bovis; Petroff; decontamination
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