title: Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies.
abstract: We standardized a method to evaluate the growth kinetics of Mycobacterium tuberculosis by measuring quantitatively the reduction of resazurin by spectrophotometry. Growth curves and the rate of growth of twenty-one M. tuberculosis clinical isolates were determined. The method showed technical simplicity and is inexpensive to assess the fitness of each isolate.
authors.affiliation: Mycobacteriology Unit, Institute of Tropical Medicine Antwerp, , Nationalestraat 155, B-2000, Antwerp , Belgium.
authors.forename: Andrea
authors.initials: A
authors.lastname: von Groll
authors.forename: Anandi
authors.initials: A
authors.lastname: Martin
authors.forename: Françoise
authors.initials: F
authors.lastname: Portaels
authors.forename: Pedro Eduardo Almeida
authors.initials: PE
authors.lastname: da Silva
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Palomino
keywords: Fitness; Growth kinetics; Mycobacterium tuberculosis; Resazurin
references.citation: J Bacteriol. 1999 Jul;181(14):4326-33
references.pmid: 10400591
references.citation: J Clin Microbiol. 1983 Aug;18(2):384-8
references.pmid: 6194175
references.citation: J Clin Microbiol. 1996 Jun;34(6):1565-7
references.pmid: 8735121
references.citation: Int J Androl. 2004 Feb;27(1):57-62
references.pmid: 14718048
references.citation: J Antimicrob Chemother. 2007 Aug;60(2):288-93
references.pmid: 17586560
references.citation: Appl Environ Microbiol. 1988 Apr;54(4):910-6
references.pmid: 3377502
references.citation: Tuberculosis (Edinb). 2004;84(3-4):218-27
references.pmid: 15207491
references.citation: J Clin Microbiol. 1983 Sep;18(3):689-96
references.pmid: 6195181
references.citation: Braz J Microbiol. 2008 Jan;39(1):16-20
references.pmid: 24031169
references.citation: J Infect Dis. 1998 Apr;177(4):1030-5
references.pmid: 9534978
references.citation: J Clin Microbiol. 1998 Sep;36(9):2752-4
references.pmid: 9705430
references.citation: J Clin Microbiol. 1983 Jun;17(6):1066-73
references.pmid: 6348076
references.citation: Antimicrob Agents Chemother. 2003 Nov;47(11):3616-9
references.pmid: 14576129
references.citation: Int J Tuberc Lung Dis. 2000 Sep;4(9):866-70
references.pmid: 10985656
references.citation: FEMS Immunol Med Microbiol. 2004 Nov 1;42(3):281-90
references.pmid: 15477041
references.citation: Antimicrob Agents Chemother. 2002 Aug;46(8):2720-2
references.pmid: 12121966
references.citation: Zentralbl Veterinarmed B. 1991 Jul;38(5):358-72
references.pmid: 1927150
references.citation: Eur J Biochem. 2000 Sep;267(17):5421-6
references.pmid: 10951200