title: Hot regions of noninterfering crossovers coexist with a nonuniformly interfering pathway in Arabidopsis thaliana.
abstract: In most organisms that have been studied, crossovers formed during meiosis exhibit interference: nearby crossovers are rare. Here we provide an in-depth study of crossover interference in Arabidopsis thaliana, examining crossovers genome-wide in >1500 backcrosses for both male and female meiosis. This unique data set allows us to take a two-pathway modeling approach based on superposing a fraction p of noninterfering crossovers and a fraction (1 - p) of interfering crossovers generated using the gamma model characterized by its interference strength nu. Within this framework, we fit the two-pathway model to the data and compare crossover interference strength between chromosomes and then along chromosomes. We find that the interfering pathway has markedly higher interference strength nu in female than in male meiosis and also that male meiosis has a higher proportion p of noninterfering crossovers. Furthermore, we test for possible intrachromosomal variations of nu and p. Our conclusion is that there are clear differences between left and right arms as well as between central and peripheral regions. Finally, statistical tests unveil a genome-wide picture of small-scale heterogeneities, pointing to the existence of hot regions in the genome where crossovers form preferentially without interference.
authors.affiliation: Institut National de la Recherche Agronomique, Unité Mixte de Recherche de Génétique Végétale, Université Paris-Sud, 91190 Gif-sur-Yvette, France.
authors.forename: Sayantani
authors.initials: S
authors.lastname: Basu-Roy
authors.forename: Franck
authors.initials: F
authors.lastname: Gauthier
authors.forename: Laurène
authors.initials: L
authors.lastname: Giraut
authors.forename: Christine
authors.initials: C
authors.lastname: Mézard
authors.forename: Matthieu
authors.initials: M
authors.lastname: Falque
authors.forename: Olivier C
authors.initials: OC
authors.lastname: Martin
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D032461:Chromosomes, Plant / Q000235:genetics; D003434:Crossing Over, Genetic; D018745:Genome, Plant; D008540:Meiosis / Q000235:genetics; D008957:Models, Genetic
keywords: crossover interference; heterogeneity; nonuniformity
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