title: Presence in the pre-surgical fine-needle aspiration of potential thyroid biomarkers previously identified in the post-surgical one.
abstract: Fine-needle aspiration biopsy (FNA) is usually applied to distinguish benign from malignant thyroid nodules. However, cytological analysis cannot always allow a proper diagnosis. We believe that the improvement of the diagnostic capability of pre-surgical FNA could avoid unnecessary thyroidectomy. In a previous study, we performed a proteome analysis to examine FNA collected after thyroidectomy. With the present study, we examined the applicability of these results on pre-surgical FNA. We collected pre-surgical FNA from 411 consecutive patients, and to obtain a correct comparison with our previous results, we processed only benign (n=114), papillary classical variant (cPTC) (n=34) and papillary tall cell variant (TcPTC) (n=14) FNA. We evaluated levels of five proteins previously found up-regulated in thyroid cancer with respect to benign nodules. ELISA and western blot (WB) analysis were used to assay levels of L-lactate dehydrogenase B chain (LDHB), Ferritin heavy chain, Ferritin light chain, Annexin A1 (ANXA1), and Moesin in FNA. ELISA assays and WB analysis confirmed the increase of LDHB, Moesin, and ANXA1 in pre-surgical FNA of thyroid papillary cancer. Sensitivity and specificity of ANXA1 were respectively 87 and 94% for cPTC, 85 and 100% for TcPTC. In conclusion, a proteomic analysis of FNA from patients with thyroid nodules may help to distinguish benign versus malignant thyroid nodules. Moreover, ANXA1 appears to be an ideal candidate given the high sensitivity and specificity obtained from ROC curve analysis.
authors.affiliation: Department of Pharmacy, University of Pisa, Pisa, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Ciregia
authors.forename: Laura
authors.initials: L
authors.lastname: Giusti
authors.forename: Angelo
authors.initials: A
authors.lastname: Molinaro
authors.forename: Filippo
authors.initials: F
authors.lastname: Niccolai
authors.forename: Patrizia
authors.initials: P
authors.lastname: Agretti
authors.forename: Teresa
authors.initials: T
authors.lastname: Rago
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Di Coscio
authors.forename: Paolo
authors.initials: P
authors.lastname: Vitti
authors.forename: Fulvio
authors.initials: F
authors.lastname: Basolo
authors.forename: Pietro
authors.initials: P
authors.lastname: Iacconi
authors.forename: Massimo
authors.initials: M
authors.lastname: Tonacchera
authors.forename: Antonio
authors.initials: A
authors.lastname: Lucacchini
mesh_terms: D017305:Annexin A1 / Q000378:metabolism; D044963:Biopsy, Fine-Needle / Q000379:methods*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D012680:Sensitivity and Specificity; D013961:Thyroid Gland / Q000378:metabolism* / Q000473:pathology; D013964:Thyroid Neoplasms / Q000175:diagnosis / Q000378:metabolism; D016606:Thyroid Nodule / Q000175:diagnosis / Q000378:metabolism
chemical_list: D017305:Annexin A1
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