title: Integrated analysis of global mRNA and protein expression data in HEK293 cells overexpressing PRL-1.
abstract: BACKGROUND
  The protein tyrosine phosphatase PRL-1 represents a putative oncogene with wide-ranging cellular effects. Overexpression of PRL-1 can promote cell proliferation, survival, migration, invasion, and metastasis, but the underlying mechanisms by which it influences these processes remain poorly understood.
  
  
  METHODS
  To increase our comprehension of PRL-1 mediated signaling events, we employed transcriptional profiling (DNA microarray) and proteomics (mass spectrometry) to perform a thorough characterization of the global molecular changes in gene expression that occur in response to stable PRL-1 overexpression in a relevant model system (HEK293).
  
  
  RESULTS
  Overexpression of PRL-1 led to several significant changes in the mRNA and protein expression profiles of HEK293 cells. The differentially expressed gene set was highly enriched in genes involved in cytoskeletal remodeling, integrin-mediated cell-matrix adhesion, and RNA recognition and splicing. In particular, members of the Rho signaling pathway and molecules that converge on this pathway were heavily influenced by PRL-1 overexpression, supporting observations from previous studies that link PRL-1 to the Rho GTPase signaling network. In addition, several genes not previously associated with PRL-1 were found to be significantly altered by its expression. Most notable among these were Filamin A, RhoGDIα, SPARC, hnRNPH2, and PRDX2.
  
  
  CONCLUSIONS
  This systems-level approach sheds new light on the molecular networks underlying PRL-1 action and presents several novel directions for future, hypothesis-based studies.
authors.affiliation: Department of Biology, Indiana University-Purdue University Indianapolis, Indianapolis, Indiana, United States of America.
authors.forename: Carmen M
authors.initials: CM
authors.lastname: Dumaual
authors.forename: Boyd A
authors.initials: BA
authors.lastname: Steere
authors.forename: Chad D
authors.initials: CD
authors.lastname: Walls
authors.forename: Mu
authors.initials: M
authors.lastname: Wang
authors.forename: Zhong-Yin
authors.initials: ZY
authors.lastname: Zhang
authors.forename: Stephen K
authors.initials: SK
authors.lastname: Randall
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018797:Cell Cycle Proteins; D008565:Membrane Proteins; D011506:Proteins; D012333:RNA, Messenger; C490198:PTP4A1 protein, human; D017027:Protein Tyrosine Phosphatases
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