title: A histochemical study of the posterior silk glands of Bombyx mori during metamorphosis from larvae to pupae using frozen sections.
abstract: The fine structures of the whole bodies and the posterior silk glands of Bombyx mori during metamorphosis from larvae to pupae in the cocoon were preserved virtually without damage when frozen sections were prepared using an adhesive plastic film. We used frozen sections for histochemical and enzyme histochemistry to characterize the metamorphosis of the posterior silk glands. Frozen sections were stained with DAPI to observe nuclear changes, examined using the TUNEL method to detect DNA fragments, and investigated using in situ hybridization to detect B. mori caspase expression. Both DNA fragments and expression of B. mori caspase increased with progressing metamorphosis. The degeneration of the posterior silk gland during metamorphosis appears to be an apoptotic event.
authors.affiliation: College of Bioresource Sciences, Nihon University , Kameino 1866, Fujisawa 252-0880.
authors.forename: K
authors.initials: K
authors.lastname: Kawamoto
authors.forename: T
authors.initials: T
authors.lastname: Kawamoto
authors.forename: H
authors.initials: H
authors.lastname: Shiba
authors.forename: K
authors.initials: K
authors.lastname: Hosono
mesh_terms: D000818:Animals; D017209:Apoptosis; D012831:Bombyx / Q000033:anatomy & histology / Q000201:enzymology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D020169:Caspases / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D017403:In Situ Hybridization; D020287:In Situ Nick-End Labeling; D007814:Larva; D008675:Metamorphosis, Biological / Q000235:genetics / Q000502:physiology*; D016133:Polymerase Chain Reaction; D011679:Pupa
chemical_list: D020169:Caspases