title: Shifts in the microbial community composition of Gulf Coast beaches following beach oiling.
abstract: Microorganisms associated with coastal sands serve as a natural biofilter, providing essential nutrient recycling in nearshore environments and acting to maintain coastal ecosystem health. Anthropogenic stressors often impact these ecosystems, but little is known about whether these disturbances can be identified through microbial community change. The blowout of the Macondo Prospect reservoir on April 20, 2010, which released oil hydrocarbons into the Gulf of Mexico, presented an opportunity to examine whether microbial community composition might provide a sensitive measure of ecosystem disturbance. Samples were collected on four occasions, beginning in mid-June, during initial beach oiling, until mid-November from surface sand and surf zone waters at seven beaches stretching from Bay St. Louis, MS to St. George Island, FL USA. Oil hydrocarbon measurements and NOAA shoreline assessments indicated little to no impact on the two most eastern beaches (controls). Sequence comparisons of bacterial ribosomal RNA gene hypervariable regions isolated from beach sands located to the east and west of Mobile Bay in Alabama demonstrated that regional drivers account for markedly different bacterial communities. Individual beaches had unique community signatures that persisted over time and exhibited spatial relationships, where community similarity decreased as horizontal distance between samples increased from one to hundreds of meters. In contrast, sequence analyses detected larger temporal and less spatial variation among the water samples. Superimposed upon these beach community distance and time relationships, was increased variability in bacterial community composition from oil hydrocarbon contaminated sands. The increased variability was observed among the core, resident, and transient community members, indicating the occurrence of community-wide impacts rather than solely an overprinting of oil hydrocarbon-degrading bacteria onto otherwise relatively stable sand population structures. Among sequences classified to genus, Alcanivorax, Alteromonas, Marinobacter, Winogradskyella, and Zeaxanthinibacter exhibited the largest relative abundance increases in oiled sands.
authors.affiliation: School of Freshwater Sciences, Great Lakes WATER Institute, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, United States of America.
authors.forename: Ryan J
authors.initials: RJ
authors.lastname: Newton
authors.forename: Susan M
authors.initials: SM
authors.lastname: Huse
authors.forename: Hilary G
authors.initials: HG
authors.lastname: Morrison
authors.forename: Colin S
authors.initials: CS
authors.lastname: Peake
authors.forename: Mitchell L
authors.initials: ML
authors.lastname: Sogin
authors.forename: Sandra L
authors.initials: SL
authors.lastname: McLellan
mesh_terms: D001493:Bathing Beaches; D017753:Ecosystem; D041965:Flavobacteriaceae / Q000145:classification / Q000235:genetics*; D020563:Gammaproteobacteria / Q000145:classification / Q000235:genetics*; D016946:Gram-Negative Aerobic Rods and Cocci / Q000145:classification / Q000235:genetics*; D060851:Gulf of America; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D006838:Hydrocarbons / Q000032:analysis / Q000378:metabolism; D059013:Microbial Consortia / Q000235:genetics*; D059392:Petroleum Pollution; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000145:classification / Q000235:genetics*; D012623:Seawater; D017422:Sequence Analysis, DNA; D014481:United States
chemical_list: D006838:Hydrocarbons; D012336:RNA, Ribosomal, 16S
references.citation: PLoS Genet. 2008 Nov;4(11):e1000255
references.pmid: 19023400
references.citation: Appl Environ Microbiol. 2011 Oct;77(19):6972-81
references.pmid: 21803887
references.citation: Environ Microbiol. 2007 Jan;9(1):177-86
references.pmid: 17227422
references.citation: Environ Microbiol. 2005 Feb;7(2):281-93
references.pmid: 15658995
references.citation: Microb Ecol. 2003 Oct;46(3):322-36
references.pmid: 14502411
references.citation: Appl Environ Microbiol. 2008 Jul;74(14):4440-53
references.pmid: 18487394
references.citation: PLoS One. 2011;6(9):e24570
references.pmid: 21931760
references.citation: ISME J. 2009 Jul;3(7):780-91
references.pmid: 19340087
references.citation: Ecol Lett. 2006 Mar;9(3):311-8
references.pmid: 16958897
references.citation: Appl Environ Microbiol. 2009 Apr;75(7):2221-9
references.pmid: 19201952
references.citation: Bioinformatics. 2011 Aug 15;27(16):2194-200
references.pmid: 21700674
references.citation: Science. 2011 May 27;332(6033):1079-82
references.pmid: 21527677
references.citation: Curr Opin Biotechnol. 2004 Jun;15(3):205-14
references.pmid: 15193328
references.citation: Microb Ecol. 2005 Apr;49(3):367-78
references.pmid: 16003476
references.citation: Appl Environ Microbiol. 2011 Nov;77(22):7962-74
references.pmid: 21948834
references.citation: PLoS Biol. 2007 Mar;5(3):e77
references.pmid: 17355176
references.citation: Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):13104-9
references.pmid: 16938845
references.citation: Environ Microbiol. 2009 Dec;11(12):3132-9
references.pmid: 19659500
references.citation: Appl Environ Microbiol. 2006 Sep;72(9):5689-701
references.pmid: 16957183
references.citation: Curr Opin Biotechnol. 2007 Jun;18(3):257-66
references.pmid: 17493798
references.citation: Nat Rev Microbiol. 2006 Mar;4(3):173-82
references.pmid: 16489346
references.citation: Appl Environ Microbiol. 1998 Jul 1;64(7):2691-6
references.pmid: 9647850
references.citation: Bioinformatics. 2010 Oct 1;26(19):2460-1
references.pmid: 20709691
references.citation: Nucleic Acids Res. 2007;35(21):7188-96
references.pmid: 17947321
references.citation: Environ Microbiol. 2012 Jan;14(1):4-12
references.pmid: 22004523
references.citation: Appl Environ Microbiol. 2009 Jun;75(11):3407-18
references.pmid: 19376924
references.citation: Environ Microbiol. 2000 Oct;2(5):542-54
references.pmid: 11233162
references.citation: Genome Biol. 2007;8(7):R143
references.pmid: 17659080
references.citation: Microbiol Mol Biol Rev. 2011 Mar;75(1):14-49
references.pmid: 21372319
references.citation: ISME J. 2012 Mar;6(3):542-53
references.pmid: 21975598
references.citation: ISME J. 2012 Feb;6(2):298-308
references.pmid: 21850055
references.citation: Science. 2012 Feb 10;335(6069):671-6
references.pmid: 22323811
references.citation: Biometrics. 2006 Jun;62(2):361-71
references.pmid: 16918900