title: Microbial metabolomic fingerprinting in urine after regular dealcoholized red wine consumption in humans.
abstract: The regular consumption of dealcoholized red wine (DRW) has demonstrated benefits in cardiovascular risk factors. The analysis of phenolic metabolites formed in the organism, especially those that could come from microbiota metabolism, would help to understand these benefits. The aim of this study was to determine the widest urinary metabolomic fingerprinting of phenolics and microbial-derived phenolic acids (n = 61) after regular intake of DRW in men at high cardiovascular risk by UPLC-MS/MS using a targeted approach. Up to 49 metabolites, including phase II and microbial phenolic metabolites, increased after DRW consumption compared to baseline (P < 0.05). The highest percentage of increase was found for microbial metabolites from anthocyanin degradation such as syringic, p-coumaric, gallic acids and pyrogallol and from flavan-3-ols degradation such as hydroxyphenylvalerolactones and (epi)catechins. These findings provide the most complete metabolic fingerprinting after wine consumption, amplifying the spectrum of microbial derived metabolites and their potential bioactivity related with health benefits.
authors.affiliation: Biomarkers and Nutritional & Food Metabolomics Research Group, Nutrition and Food Science Department, XaRTA, INSA, Pharmacy Faculty, University of Barcelona , Avenida Joan XXIII s/n, 08028 Barcelona, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Boto-Ordóñez
authors.forename: Mireia
authors.initials: M
authors.lastname: Urpi-Sarda
authors.forename: María Isabel
authors.initials: MI
authors.lastname: Queipo-Ortuño
authors.forename: Dolores
authors.initials: D
authors.lastname: Corella
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Tinahones
authors.forename: Ramon
authors.initials: R
authors.lastname: Estruch
authors.forename: Cristina
authors.initials: C
authors.lastname: Andres-Lacueva
mesh_terms: D000328:Adult; D000368:Aged; D001419:Bacteria / Q000378:metabolism*; D002318:Cardiovascular Diseases / Q000378:metabolism / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000378:metabolism*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D055432:Metabolomics; D064307:Microbiota; D008875:Middle Aged; D010636:Phenols / Q000378:metabolism; D014556:Urine / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
chemical_list: D010636:Phenols; D000431:Ethanol