title: Mild strain cross protection of tristeza: a review of research to protect against decline on sour orange in Florida.
abstract: Tristeza, caused by Citrus tristeza virus (CTV), has long been present in Florida but outbreaks of decline on sour orange rootstock were occasional events until the late 1970s. Sour orange rootstock was valued for the high quality of fruit produced and was widely used because of its tolerance of citrus blight, a disease of unknown etiology. Research was directed towards the selection and screening of mild strains of CTV which could protect against sour orange decline strains. Following the introduction of Toxoptera citricida (also known as the brown citrus aphid) in 1995 there was a greater concern for maintaining production of existing blocks of citrus on sour orange rootstock. Availability of the CTV genome sequence around the same time as well as molecular characterization of in planta CTV populations led to the selection of mild CTV isolates which when inoculated into existing field trees, extended the productive life of the groves and enabled a more graduate replanting of trees on CTV-tolerant rootstocks. The history of CTV in Florida and the methods developed to select mild isolates for use for mild strain cross protection will be reviewed.
authors.affiliation: National Clonal Germplasm Repository for Citrus and Dates, Agricultural Research Service, United States Department of Agriculture Riverside, CA, USA.
authors.forename: Richard F
authors.initials: RF
authors.lastname: Lee
authors.forename: Manjunath L
authors.initials: ML
authors.lastname: Keremane
keywords: biological indexing; mild isolate selection; serology; stem pitting; strain differentiation
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