title: Development of a chemically defined medium for the production of the antibiotic platensimycin by Streptomyces platensis.
abstract: The actinomycete Streptomyces platensis produces two compounds that display antibacterial activity: platensimycin and platencin. These compounds were discovered by the Merck Research Laboratories, and a complex insoluble production medium was reported. We have used this medium as our starting point in our studies. In a previous study, we developed a semi-defined production medium, i.e., PM5. In the present studies, by varying the concentration of the components of PM5, we were able to develop a superior semi-defined medium, i.e., PM6, which contains a higher concentration of lactose. Versions of PM6, containing lower concentrations of all components, were also found to be superior to PM5. The new semi-defined production media contain dextrin, lactose, MOPS buffer, and ammonium sulfate in different concentrations. We determined antibiotic production capabilities using agar diffusion assays and chemical assays via thin-layer silica chromatography and high-performance liquid chromatography. We reduced crude nutrient carryover from the seed medium by washing the cells with distilled water. Using these semi-defined media, we determined that addition of the semi-defined component soluble starch stimulated antibiotic production and that it and dextrin could both be replaced with glucose, resulting in the chemically defined medium, PM7.
authors.forename: Maria
authors.initials: M
authors.lastname: Falzone
authors.forename: Evan
authors.initials: E
authors.lastname: Martens
authors.forename: Heather
authors.initials: H
authors.lastname: Tynan
authors.forename: Christian
authors.initials: C
authors.lastname: Maggio
authors.forename: Samantha
authors.initials: S
authors.lastname: Golden
authors.forename: Vasyl
authors.initials: V
authors.lastname: Nayda
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Crespo
authors.forename: Gregory
authors.initials: G
authors.lastname: Inamine
authors.forename: Michael
authors.initials: M
authors.lastname: Gelber
authors.forename: Ryan
authors.initials: R
authors.lastname: Lemence
authors.forename: Nicholas
authors.initials: N
authors.lastname: Chiappini
authors.forename: Emily
authors.initials: E
authors.lastname: Friedman
authors.forename: Ben
authors.initials: B
authors.lastname: Shen
authors.forename: Vincent
authors.initials: V
authors.lastname: Gullo
authors.forename: Arnold L
authors.initials: AL
authors.lastname: Demain
mesh_terms: D000218:Adamantane / Q000378:metabolism*; D062365:Aminobenzoates / Q000378:metabolism*; D000813:Anilides / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003470:Culture Media / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D013302:Streptomyces / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D062365:Aminobenzoates; D000813:Anilides; D000900:Anti-Bacterial Agents; D003470:Culture Media; D000218:Adamantane; C510909:platensimycin