title: Exfoliative cytology of oral epithelial cells from patients with type 2 diabetes: cytomorphometric analysis.
abstract: This research objective is to identify cytomorphometrical changes using exfoliative cytology (EC) and later Papanicolaou (Pap) staining, for oral epithelial cells of patients with type 2 diabetes (DM2) (n = 30), while being compared to patients without the disease (n = 30). Additionally, we investigated an association between cellular changes and salivary flow levels; relationship that until now has not been reported. Results show that the cell diameter and the nuclear-cytoplasmic ratio was significantly higher compared to those patients without the disease (p ≤ 0.001 Student and Welch test). Decreased salivary flow was significantly associated with increased cell diameter and nuclear-cytoplasmic ratio (p ≤ 0.001 ANOVA with Tukey test). Evidence and clinical observations show that DM2 and decreased salivary flow are related to detectable cytomorphometrical changes in exfoliated cells, which may extend the horizon of this cytological technique.
authors.affiliation: Unit of Histology and Embryology, Department of Basic Biomedical Sciences, Faculty of Health Sciences, University of Talca Talca, Chile ; Biomedical Sciences Master Program, Oral Pathology mention, University of Talca Talca, Chile.
authors.forename: César
authors.initials: C
authors.lastname: Rivera
authors.forename: Camila
authors.initials: C
authors.lastname: Núñez-de-Mendoza
keywords: Diabetes mellitus type 2; cytological techniques; mouth mucosa; salivary flow; xerostomia
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