title: Electrochemiluminescence detection of c-Myc mRNA in breast cancer cells on a wireless bipolar electrode.
abstract: Electrochemiluminescence (ECL) on bipolar electrode (BPE) is a sensitive, portable, and low-cost approach which has been employed to detect DNA and proteins. Here, we develop an ultrasensitive method for intracellular mRNA assay based on mRNA-mediated reporter DNA liberation and Ru(bpy)3(2+)-conjugated silica nanoparticles (RuSi@Ru(bpy)3(2+)) tag-based signal amplification.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China.
authors.forename: Mei-Sheng
authors.initials: MS
authors.lastname: Wu
authors.forename: Guang-sheng
authors.initials: GS
authors.lastname: Qian
authors.forename: Jing-Juan
authors.initials: JJ
authors.lastname: Xu
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Chen
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001943:Breast Neoplasms; D055664:Electrochemical Techniques; D004566:Electrodes; D005260:Female; D006801:Humans; D008163:Luminescent Measurements; D061986:MCF-7 Cells; D016271:Proto-Oncogene Proteins c-myc / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D059015:Wireless Technology
chemical_list: C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger