title: Overexpression of hsa-miR-125b during osteoblastic differentiation does not influence levels of Runx2, osteopontin, and ALPL gene expression.
abstract: Multipotent mesenchymal stromal cells (MSCs) were first isolated from bone marrow and then from various adult tissues including placenta, cord blood, deciduous teeth, and amniotic fluid. MSCs are defined or characterized by their ability to adhere to plastic, to express specific surface antigens, and to differentiate into osteogenic, chondrogenic, adipogenic, and myogenic lineages. Although the molecular mechanisms that control MSC proliferation and differentiation are not well understood, the involvement of microRNAs has been reported. In the present study, we investigated the role of miR-125b during osteoblastic differentiation in humans. We found that miR-125b increased during osteoblastic differentiation, as well as Runx2 and ALPL genes. To study whether the gain or loss of miR-125b function influenced osteoblastic differentiation, we transfected MSCs with pre-miR-125b or anti-miR-125b and cultured the transfected cells in an osteoblastic differentiation medium. After transfection, no change was observed in osteoblastic differentiation, and Runx2, OPN, and ALPL gene expression were not changed. These results suggest that the gain or loss of miR-125b function does not influence levels of Runx2, OPN, and ALPL during osteoblastic differentiation.
authors.affiliation: Instituto Nacional de Ciência e Tecnologia em Células-Tronco e Terapia Celular, Centro Regional de Hemoterapia de Ribeirão Preto, Ribeirão PretoSP, Brasil.
authors.forename: M T
authors.initials: MT
authors.lastname: Pinto
authors.forename: L D F
authors.initials: LD
authors.lastname: Nicolete
authors.forename: E S
authors.initials: ES
authors.lastname: Rodrigues
authors.forename: P V B
authors.initials: PV
authors.lastname: Palma
authors.forename: M D
authors.initials: MD
authors.lastname: Orellana
authors.forename: S
authors.initials: S
authors.lastname: Kashima
authors.forename: D T
authors.initials: DT
authors.lastname: Covas
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism*; D000943:Antigens, Differentiation / Q000302:isolation & purification; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000502:physiology*; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis / Q000502:physiology; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism*; D061251:Primary Cell Culture; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D000943:Antigens, Differentiation; D050659:Core Binding Factor Alpha 1 Subunit; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; C493709:RUNX2 protein, human; D053495:Osteopontin; C548267:ALPL protein, human; D000469:Alkaline Phosphatase
references.citation: J Bone Miner Res. 2003 Apr;18(4):696-704
references.pmid: 12674330
references.citation: Placenta. 2005 Sep 19;:
references.pmid: 16174531
references.citation: Transfusion. 1971 Nov-Dec;11(6):345-9
references.pmid: 5136066
references.citation: Cytotherapy. 2006;8(4):315-7
references.pmid: 16923606
references.citation: Stem Cells. 2004;22(4):625-34
references.pmid: 15277708
references.citation: ScientificWorldJournal. 2012;2012:793823
references.pmid: 22500143
references.citation: Proc Natl Acad Sci U S A. 2011 Apr 12;108(15):6139-44
references.pmid: 21444814
references.citation: Mol Biol Cell. 2011 Nov;22(21):3955-61
references.pmid: 21880893
references.citation: Cell. 2004 Jan 23;116(2):281-97
references.pmid: 14744438
references.citation: Nat Rev Immunol. 2008 Sep;8(9):726-36
references.pmid: 19172693
references.citation: J Biol Chem. 2007 Jan 12;282(2):1479-86
references.pmid: 17110380
references.citation: Genome Res. 2009 Jan;19(1):92-105
references.pmid: 18955434
references.citation: Blood. 2003 Aug 15;102(4):1548-9
references.pmid: 12900350
references.citation: Stem Cells Dev. 2011 Feb;20(2):259-67
references.pmid: 20486779
references.citation: Histochemistry. 1978 Jul 12;56(3-4):177-85
references.pmid: 689915
references.citation: Development. 2005 Nov;132(21):4645-52
references.pmid: 16224044
references.citation: Nucleic Acids Res. 2001 May 1;29(9):e45
references.pmid: 11328886
references.citation: Biochem Biophys Res Commun. 2008 Apr 4;368(2):267-72
references.pmid: 18230348