title: Strong cation exchange chiral stationary phase--a comparative study in high-performance liquid chromatography and subcritical fluid chromatography.
abstract: The performance of a strong cation exchange-type (SCX) chiral stationary phase (CSP) was evaluated with subcritical fluid chromatography (subFC) and high performance liquid chromatography (HPLC). The chromatographic conditions in subFC were optimized by changing the amount of polar organic modifier, concentration of a basic additive in the modifier, system pressure and temperature. In this way the concentration of in situ formed transient ionic species could be varied. The gradual change of the concentration of the transient buffer, i.e. gradient elution conditions in subFC, was found beneficial for separation of a mixture of racemic compounds. The strength and amount of the in situ formed buffer was estimated on the basis of comparative experiments in subSFC and HPLC.
authors.affiliation: Department of Analytical Chemistry, University of Vienna, Währinger Strasse 38, A-1090 Vienna, Austria.
authors.forename: Denise
authors.initials: D
authors.lastname: Wolrab
authors.forename: Pavla
authors.initials: P
authors.lastname: Macíková
authors.forename: Mario
authors.initials: M
authors.lastname: Boras
authors.forename: Michal
authors.initials: M
authors.lastname: Kohout
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Lindner
mesh_terms: D000588:Amines / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D000641:Ammonia / Q000737:chemistry; D002255:Carbonic Acid / Q000737:chemistry; D002412:Cations / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000295:instrumentation* / Q000379:methods; D025924:Chromatography, Supercritical Fluid / Q000295:instrumentation* / Q000379:methods; D011312:Pressure; D013237:Stereoisomerism; D013696:Temperature
chemical_list: D000588:Amines; D002412:Cations; D002255:Carbonic Acid; D000641:Ammonia
keywords: Chiral cation exchange; Enantiomer separation; Gradient elution; High-performance liquid chromatography; Subcritical fluid chromatography; Transient buffer salts