title: A simplified experimental model of large-for-size liver transplantation in pigs.
abstract: OBJECTIVE
  The ideal ratio between liver graft mass and recipient body weight for liver transplantation in small infants is unknown; however, if this ratio is over 4%, a condition called large-for-size may occur. Experimental models of large-for-size liver transplants have not been described in the literature. In addition, orthotopic liver transplantation is marked by high morbidity and mortality rates in animals due to the clamping of the venous splanchnic system. Therefore, the objective of this study was to create a porcine model of large-for-size liver transplantation with clamping of the supraceliac aorta during the anhepatic phase as an alternative to venovenous bypass.
  
  
  METHODS
  Fourteen pigs underwent liver transplantation with whole-liver grafts without venovenous bypass and were divided into two experimental groups: the control group, in which the weights of the donors were similar to the weights of the recipients; and the large-for-size group, in which the weights of the donors were nearly 2 times the weights of the recipients. Hemodynamic data, the results of serum biochemical analyses and histological examination of the transplanted livers were collected.
  
  
  RESULTS
  The mortality rate in both groups was 16.5% (1/7). The animals in the large-for-size group had increased serum levels of potassium, sodium, aspartate aminotransferase and alanine aminotransferase after graft reperfusion. The histological analyses revealed that there were no significant differences between the groups.
  
  
  CONCLUSIONS
  This transplant method is a feasible experimental model of large-for-size liver transplantation.
authors.affiliation: Faculdade de Medicina da Universidade de São Paulo, Pediatric Liver Transplantation Unit and Laboratory of Research in Pediatric Surgery (LIM 30), Pediatric Surgery Division, São PauloSP, Brazil.
authors.forename: António José Gonçalves
authors.initials: AJ
authors.lastname: Leal
authors.forename: Ana Cristina Aoun
authors.initials: AC
authors.lastname: Tannuri
authors.forename: Alessandro Rodrigo
authors.initials: AR
authors.lastname: Belon
authors.forename: Raimundo Renato Nunes
authors.initials: RR
authors.lastname: Guimarães
authors.forename: Maria Cecília Mendonça
authors.initials: MC
authors.lastname: Coelho
authors.forename: Josiane de
authors.initials: Jd
authors.lastname: Oliveira Gonçalves
authors.forename: Suellen Serafini
authors.initials: SS
authors.lastname: Sokol
authors.forename: Evandro Sobroza
authors.initials: ES
authors.lastname: De Melo
authors.forename: José Pinhata
authors.initials: JP
authors.lastname: Otoch
authors.forename: Uenis
authors.initials: U
authors.lastname: Tannuri
mesh_terms: D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001835:Body Weight; D005240:Feasibility Studies; D006439:Hemodynamics; D008099:Liver / Q000033:anatomy & histology*; D016031:Liver Transplantation / Q000379:methods*; D023421:Models, Animal; D009929:Organ Size; D011188:Potassium / Q000097:blood; D015203:Reproducibility of Results; D012964:Sodium / Q000097:blood; D013552:Swine; D013997:Time Factors
chemical_list: D012964:Sodium; D001219:Aspartate Aminotransferases; D011188:Potassium
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