title: Antitumorigenic effect of dietary natural compounds via lipid metabolism modulation in Apc(Min/+) mice.
abstract: OBJECTIVE
  The aim of the present study was to evaluate the effects of a diet supplementation with either olive oil, or n-3 or n-6-polyunsaturatedFatty acids (PUFAs) on tumour development and gene expression for lipogenic enzymes in Apc(Min/+) mice.
  
  
  METHODS
  In the control group, the mice received a standard diet, the OO group was fed on a diet with 12% olive oil, the OM-3 group with 12% salmon fish rich in n-3 PUFAs, the OM-6 group with 12% oenothera oil rich in n-6 PUFAs. Gene expression of lipogenic enzymes was evaluated by real-time reverse transcription polymerase chain reaction.
  
  
  RESULTS
  All mice in the treated groups presented a reduction in total intestinal polyp number and load, which was particularly marked in the OM-3 group. Treated mice showed an induction of low density lipoprotein receptor gene expression and a significant reduction of expression of lipogenic gene.
  
  
  CONCLUSIONS
  Our data provide new insights into the mechanism of cell growth inhibition and apoptotic regulation by dietary olive oil and PUFAs in Apc(Min/+) mice.
authors.affiliation: Laboratory of Nutritional Biochemistry, National Institute for Digestive Diseases, Castellana Grotte, Bari, Italy. gabriella.caruso@irccsdebellis.it.
authors.forename: Maria
authors.initials: M
authors.lastname: Notarnicola
authors.forename: Valeria
authors.initials: V
authors.lastname: Tutino
authors.forename: Angela
authors.initials: A
authors.lastname: Tafaro
authors.forename: Francesca
authors.initials: F
authors.lastname: Amatulli
authors.forename: Maria Gabriella
authors.initials: MG
authors.lastname: Caruso
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001688:Biological Products / Q000494:pharmacology*; D019587:Dietary Supplements; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology; D043371:Fatty Acids, Omega-6 / Q000494:pharmacology; D020869:Gene Expression Profiling; D017491:Genes, APC; D050356:Lipid Metabolism / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D000069463:Olive Oil; D010938:Plant Oils; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D016588:Anticarcinogenic Agents; D001688:Biological Products; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D000069463:Olive Oil; D010938:Plant Oils
keywords: Olive oil; colorectal cancer; gene expression; lipid metabolism; polyunsaturated fatty acids (PUFAs)