title: Abundance and diversity of ammonia-oxidizing bacteria in relation to ammonium in a chinese shallow eutrophic urban lake.
abstract: The measures of most-probable-number and restriction fragment length polymorphism analysis were used to analyze the abundance and diversity of ammonia-oxidizing bacteria in sediment of a Chinese shallow eutrophic urban lake (Lake Yuehu). Among the 5 sampling sites, ammonia concentration in interstitial water was positively proportional not only to the content of organic matter, but also to ammonia-oxidizing bacteria numbers (at a magnitude of 10(5) cells g(-1) dry weight) in sediment significantly. Furthermore, the diversity of ammonia-oxidizing bacteria were determined by means of PCR primers targeting the amoA gene with five gene libraries created and restriction pattern analysis. The 13 restriction patterns were recorded with 4 ones being common among all sampling sites. The 8 restriction patterns including 4 unique ones were found at the site with the highest NH4 (+) concentrations in interstitial water, while, there were only common patterns without unique ones at the site with the lowest NH4 (+) concentrations in interstitial water. Phylogenetic analysis showed that the amoA fragments retrieved belong to Nitrosomonas oligotropha & ureae lineage, N. europaea lineage, N. communis lineage and Nitrosospira lineage, most of which were affiliated with the genus Nitrosomonas. The N. oligotropha & ureae-like bacteria were the dominant species. Thus, the abundance and diversity of sediment AOB is closely linked to ammonium status in eutrophic lakes.
authors.affiliation: Institute of Hydrobiology, Chinese Academy of Sciences , 7 Donghu Nan Road, Wuhan, Hubei 430072 , China ; Graduate School of the Chinese Academy of Sciences , 19 Yuquan Road, Beijing 100049 , China.
authors.forename: Shanlian
authors.initials: S
authors.lastname: Qiu
authors.forename: Guoyuan
authors.initials: G
authors.lastname: Chen
authors.forename: Yiyong
authors.initials: Y
authors.lastname: Zhou
keywords: Abundance,; Ammonia-oxidizing bacteria; Diversity.; amoA
references.citation: J Bacteriol. 1993 Apr;175(8):2436-44
references.pmid: 8468301
references.citation: Appl Environ Microbiol. 1978 Oct;36(4):584-8
references.pmid: 16345319
references.citation: Appl Environ Microbiol. 2002 Jan;68(1):73-81
references.pmid: 11772611
references.citation: J Bacteriol. 1994 Nov;176(21):6623-30
references.pmid: 7961414
references.citation: J Gen Microbiol. 1993 Jun;139 Pt 6:1147-53
references.pmid: 7689633
references.citation: J Gen Appl Microbiol. 1997 Dec;43(6):373-379
references.pmid: 12501310
references.citation: J Microbiol Methods. 2002 Jul;50(2):189-203
references.pmid: 11997169
references.citation: Syst Appl Microbiol. 1998 Jun;21(2):321-30
references.pmid: 9704117
references.citation: Appl Environ Microbiol. 1996 Feb;62(2):316-22
references.pmid: 8593035
references.citation: Environ Microbiol. 2006 May;8(5):787-803
references.pmid: 16623737
references.citation: Water Sci Technol. 2001;43(3):9-18
references.pmid: 11381937
references.citation: Appl Environ Microbiol. 2002 Oct;68(10):4751-7
references.pmid: 12324316
references.citation: Appl Environ Microbiol. 2001 Dec;67(12):5791-800
references.pmid: 11722936
references.citation: Appl Environ Microbiol. 1999 Sep;65(9):4155-62
references.pmid: 10473429
references.citation: Appl Environ Microbiol. 2002 Jan;68(1):245-53
references.pmid: 11772633
references.citation: Environ Microbiol. 2007 Sep;9(9):2375-82
references.pmid: 17686033
references.citation: Appl Environ Microbiol. 2004 Feb;70(2):1008-16
references.pmid: 14766583
references.citation: FEMS Microbiol Ecol. 2007 Jun;60(3):501-12
references.pmid: 17391333
references.citation: Antonie Van Leeuwenhoek. 2001 Jun;79(2):179-88
references.pmid: 11520004
references.citation: Appl Environ Microbiol. 1999 Nov;65(11):4855-62
references.pmid: 10543796
references.citation: Appl Microbiol Biotechnol. 2008 May;79(1):135-45
references.pmid: 18305935
references.citation: Appl Environ Microbiol. 2003 Dec;69(12):7091-100
references.pmid: 14660353
references.citation: Adv Microb Physiol. 1989;30:125-81
references.pmid: 2700538
references.citation: Water Res. 2004 Aug-Sep;38(14-15):3275-86
references.pmid: 15276744
references.citation: Appl Environ Microbiol. 1998 Aug;64(8):3042-51
references.pmid: 9687471
references.citation: Arch Microbiol. 1985 Apr;141(3):214-8
references.pmid: 4004447
references.citation: Appl Microbiol. 1975 Feb;29(2):265-8
references.pmid: 1090252
references.citation: Bioinformatics. 1998;14(9):817-8
references.pmid: 9918953
references.citation: Appl Environ Microbiol. 1997 Dec;63(12):4704-12
references.pmid: 9406389
references.citation: Syst Appl Microbiol. 1998 Mar;21(1):72-88
references.pmid: 9741112
references.citation: Evolution. 1985 Jul;39(4):783-791
references.pmid: 28561359