title: Location and characterization of female germline stem cells (FGSCs) in juvenile porcine ovary.
abstract: OBJECTIVE
  Existence of germline stem cells (GSCs) in juvenile mammalian female ovaries has been drastically debated recently since reports that adult mouse ovaries still have mitotically active germ cells have been proposed. In addition, definitive location of such female germline stem cells (FGSCs) had not been demonstrated.
  
  
  METHODS
  We segregated porcine FGSCs mechanically from ovary cortex, and tested our hypotheses by utilizing immunofluorescent staining, qRT-PCR and western blotting.
  
  
  RESULTS
  We attached emphasis to unambiguous location of FGSCs, which settle simultaneously in the theca. Dissected cells from porcine thecal layers maintained similar characteristics to mouse FGSCs and ESCs over 4-months in vitro culture.
  
  
  CONCLUSIONS
  These results may provide a new resource for the study of oogenesis and therapy for ovarian sterility.
authors.affiliation: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering and Technology, Key Lab for Animal Biotechnology of Agriculture Ministry of China, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Bai
authors.forename: M
authors.initials: M
authors.lastname: Yu
authors.forename: Y
authors.initials: Y
authors.lastname: Hu
authors.forename: P
authors.initials: P
authors.lastname: Qiu
authors.forename: W
authors.initials: W
authors.lastname: Liu
authors.forename: W
authors.initials: W
authors.lastname: Zheng
authors.forename: S
authors.initials: S
authors.lastname: Peng
authors.forename: J
authors.initials: J
authors.lastname: Hua
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D005854:Germ Cells / Q000166:cytology* / Q000473:pathology / Q000637:transplantation; D051379:Mice; D010053:Ovary / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D013552:Swine; D013724:Teratoma / Q000473:pathology
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