title: In vitro selection of BNA (LNA) aptamers.
abstract: Recently, we achieved the first in vitro selection of 2'-O,4'-C-methylene bridged/locked nucleic acid (2',4'-BNA/LNA) aptamers. High-affinity thrombin-binding aptamers (TBAs) were obtained from DNA-based libraries containing 2'-O,4'-C-methylene-bridged/linked bicyclic ribonucleotides (B/L nucleotides) in the 5'-primer region, using the method of capillary electrophoresis systematic evolution of ligands by exponential enrichment (CE-SELEX). Furthermore, a similar selection protocol could provide TBAs that contain B/L nucleotides in both primer and random regions. We review technical challenges involved in the generation of various BNA libraries using analogs of B/L nucleoside-5'-triphosphate and polymerase variants and also discuss applications of these libraries to the selection of BNA (LNA) aptamers, as well as future prospects for their therapeutic and diagnostic uses.
authors.affiliation: Graduate School of Science and Technology; Gunma University; Gunma, Japan.
authors.forename: Masayasu
authors.initials: M
authors.lastname: Kuwahara
authors.forename: Satoshi
authors.initials: S
authors.lastname: Obika
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D055029:Inverted Repeat Sequences; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics*; D052156:SELEX Aptamer Technique
chemical_list: D052157:Aptamers, Nucleotide; D009841:Oligonucleotides; C477371:locked nucleic acid
keywords: 2′,4′-BNA/LNA; Bridged (locked) nucleic acid aptamer; Capillary electrophoresis-systematic evolution of ligands by exponential enrichment (CE-SELEX); KODDNA polymerase; Xeno-nucleic acid (XNA)
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