title: Culture Conditions stimulating high γ-Linolenic Acid accumulation by Spirulina platensis.
abstract: Gamma-linolenic acid (GLA) production by Spirulina platensis under different stress-inducing conditions was studied. Submerged culture studies showed that low temperature (25°C), strong light intensity (6 klux) and primrose oil supplement (0.8%w/v) induced 13.2 mg/g, 14.6 mg/g and 13.5 mg linolenic acid per gram dry cell weight respectively. A careful observation of fatty acid profile of the cyanobacteria shows that, oleic acid and linoleic acid, in experiments with varying growth temperature and oil supplements respectively, helped in accumulating excess γ-linolenic acid. In addition, cultures grown at increasing light regimes maintained the γ-linolenic acid to the total fatty acid ratio(GLA/TFA) constant, despite any change in γ-linolenic acid content of the cyanobacteria.
authors.affiliation: Koneru Lakshmaiah College of Engineering, Vaddeswaram , Guntur-522502, A.P , India.
authors.forename: Srinivasa Reddy
authors.initials: SR
authors.lastname: Ronda
authors.forename: S S
authors.initials: SS
authors.lastname: Lele
keywords: Gamma linolenic acid; Light intensity; Oil supplementation; Temperature
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