title: Frequency of genetic polymorphisms of ADAM33 and their association with allergic rhinitis among Jordanians.
abstract: Allergic rhinitis is a chronic inflammatory disease that is assumed to be due to an interaction between different genetic and/or environmental factors. A disintegrin and metalloprotease domain 33 (ADAM33) has been extensively studied as a susceptibility gene in asthma and has been linked to bronchial hyper-responsiveness. In this study, we investigated the association between ADAM33 single nucleotide polymorphisms and the incidence of allergic rhinitis among the Jordanian population. We conducted a case-control association study on 120 adult individuals diagnosed with allergic rhinitis and 128 normal healthy controls. 8 single-nucleotide polymorphisms in ADAM33 were genotyped using PCR-RFLP method. No significant differences in the allelic frequencies of all SNPs tested between AR patients and the control volunteers were found, although S2 C/G SNP showed a tendency toward significance with P=0.06. On the genotype level significant association were found in the following genotypes: T1 AA, T1 AG, T2 GG, T2 AG, T+1 GG, T+1 AG, V4 CG, S2 CC, S2 CG, Q-1AA. Seven haplotypes were present only within AR patients and eight haplotypes were completely absent from the AR patients. Three haplotypes exhibited significant association with AR P ≤ 0.05, two of them were present only in AR patients. In conclusion, the polymorphisms in the ADAM33 gene are associated with susceptibility to AR in the Jordanian population. Furthermore, the haplotype of the tested SNPs were also associated with the risk of AR.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, The University of Jordan, Amman, Jordan. Electronic address: M.zihlif@ju.edu.jo.
authors.forename: Malek
authors.initials: M
authors.lastname: Zihlif
authors.forename: Tareq
authors.initials: T
authors.lastname: Mahafza
authors.forename: Nathir M
authors.initials: NM
authors.lastname: Obeidat
authors.forename: Tawfiq
authors.initials: T
authors.lastname: Froukh
authors.forename: Mazen
authors.initials: M
authors.lastname: Shaban
authors.forename: Fatima M
authors.initials: FM
authors.lastname: Al-Akhras
authors.forename: Nadwa
authors.initials: N
authors.lastname: Zihlif
authors.forename: Randa
authors.initials: R
authors.lastname: Naffa
mesh_terms: D051722:ADAM Proteins / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002648:Child; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D006801:Humans; D007597:Jordan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D065631:Rhinitis, Allergic; D012221:Rhinitis, Allergic, Perennial / Q000453:epidemiology / Q000235:genetics*; D055815:Young Adult
chemical_list: D051722:ADAM Proteins; C450441:ADAM33 protein, human
keywords: A disintegrin and metalloprotease domain 33; ADAM33; AR; Allergic rhinitis; Genotype; Haplotype; IGE level; IRB; IgE; Immunoglobulin E; Institutional Review Board; JUH; Jordan University Hospital; NCBI; National Center for Biotechnology Information; PCR/RFLP; Polymerase Chain Reaction/Restriction Fragment Length Polymorphism; SNPs; Single nucleotide polymorphisms