title: Production of 6-pentyl-α-pyrone by trichoderma harzianum in solid-state fermentation.
abstract: Many Trichoderma species are able to produce 6-pentyl-α-pyrone (6-PP), a lactone with coconut-like aroma. In the present work, several culture parameters were studied to enhance the production of 6-PP by Trichoderma harzianum 4040 in solid-state fermentation. Green coir powder added to a nutrient solution was used as support material for fermentation. A Plackett-Burman screening technique was applied, followed by a fractionary factorial design. The best culture conditions within the experimental domain studied were (100 g support)(-1): sucrose, 3 g; NaNO3, 0.24 g; (NH4)2SO4, 0.18 g; KH2PO4, 0.1 g; inoculum concentration, 2.2 × 10(6) spores; moisture level, 55%. The temperature established was 28°C. The fermentation under the selected conditions led to a 6-PP production six times higher (5.0 mg/g dry matter) than the initial one (0.8 mg/g dry matter) after seven days of cultivation.
authors.affiliation: Farmanguinhos, Fundação Oswaldo Cruz , Rio de Janeiro, RJ , Brasil ; Departamento de Engenharia Bioquímica, Escola de Química, Universidade Federal do Rio de Janeiro , Rio de Janeiro, RJ , Brasil.
authors.forename: Aline
authors.initials: A
authors.lastname: de Souza Ramos
authors.forename: Sorele Batista
authors.initials: SB
authors.lastname: Fiaux
authors.forename: Selma Gomes Ferreira
authors.initials: SG
authors.lastname: Leite
keywords: 6-pentyl-α-pyrone; Trichoderma harzianum; experimental design; solid-state fermentation
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