title: CD200 flow cytometric assessment and semiquantitative immunohistochemical staining distinguishes hairy cell leukemia from hairy cell leukemia-variant and other B-cell lymphoproliferative disorders.
abstract: OBJECTIVE
  To evaluate CD200 expression in B-cell proliferative disorders.
  
  
  METHODS
  We analyzed 180 recent specimens of B-cell neoplasms for CD200 expression by flow cytometric immunophenotypic analysis, which is better able to assess relative intensity of staining than immunohistochemical staining.
  
  
  RESULTS
  We found that hairy cell leukemia exhibits a high level of staining for CD200 in comparison to other B-cell lymphoproliferative disorders, including hairy cell leukemia-variant (HCL-V), marginal zone lymphoma, and lymphoplasmacytic lymphoma. We confirmed this observation by semiquantitative immunohistochemical staining.
  
  
  CONCLUSIONS
  Assessment of the CD200 expression level is helpful to distinguish HCL from HCL-V and other B-cell lymphoproliferative disorders and in the differential diagnosis of B-cell neoplasms in general.
authors.affiliation: Dept of Pathology, Brigham and Women's Hospital, 75 Francis St, Boston, MA 02115.
authors.forename: Vinodh
authors.initials: V
authors.lastname: Pillai
authors.forename: Olga
authors.initials: O
authors.lastname: Pozdnyakova
authors.forename: Karry
authors.initials: K
authors.lastname: Charest
authors.forename: Betty
authors.initials: B
authors.lastname: Li
authors.forename: Aliakbar
authors.initials: A
authors.lastname: Shahsafaei
authors.forename: David M
authors.initials: DM
authors.lastname: Dorfman
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000276:immunology / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology; D003937:Diagnosis, Differential; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D016130:Immunophenotyping; D007943:Leukemia, Hairy Cell / Q000175:diagnosis* / Q000276:immunology / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D018442:Lymphoma, B-Cell, Marginal Zone / Q000175:diagnosis* / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008258:Waldenstrom Macroglobulinemia / Q000175:diagnosis* / Q000276:immunology
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; C042739:antigens, CD200
keywords: Chronic lymphocytic leukemia; Hairy cell leukemia; Hairy cell leukemia-variant; Mantle cell lymphoma