title: Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR.
abstract: Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9%). The use of mPCR of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested.
authors.affiliation: Universidade Federal do Rio de Janeiro , Rio de Janeiro, RJ , Brasil.
authors.forename: Eduardo Eustáquio
authors.initials: EE
authors.lastname: de Souza Figueiredo
authors.forename: Ricardo Cezar Tavares
authors.initials: RC
authors.lastname: Carvalho
authors.forename: Flávia Galindo
authors.initials: FG
authors.lastname: Silvestre
authors.forename: Walter
authors.initials: W
authors.lastname: Lilenbaum
authors.forename: Leila Sousa
authors.initials: LS
authors.lastname: Fonseca
authors.forename: Joab Trajano
authors.initials: JT
authors.lastname: Silva
authors.forename: Vânia Margaret Flosi
authors.initials: VM
authors.lastname: Paschoalin
keywords: Mycobacterium bovis; bovine tuberculosis; multiplex-PCR; nasal swab
references.citation: Comp Immunol Microbiol Infect Dis. 2007 Sep;30(5-6):415-25
references.pmid: 17706284
references.citation: J R Soc Interface. 2007 Jun 22;4(14):545-51
references.pmid: 17251130
references.citation: Am J Vet Res. 1978 Jul;39(7):1233-4
references.pmid: 354437
references.citation: J Clin Microbiol. 1990 Sep;28(9):2051-8
references.pmid: 1977765
references.citation: J Clin Microbiol. 1999 Jul;37(7):2330-2
references.pmid: 10364607
references.citation: Vet Rec. 1993 Jan 16;132(3):66-7
references.pmid: 8430485
references.citation: Vet Rec. 2005 Nov 12;157(20):613-8
references.pmid: 16284329
references.citation: Zentralbl Veterinarmed B. 1998 Oct;45(8):473-9
references.pmid: 9820115
references.citation: J Infect Dis. 1990 May;161(5):977-81
references.pmid: 2109022
references.citation: Braz J Microbiol. 2009 Apr;40(2):231-3
references.pmid: 24031349
references.citation: J Clin Microbiol. 1998 Apr;36(4):1050-5
references.pmid: 9542936
references.citation: Vet Rec. 1988 Sep 24;123(13):340-3
references.pmid: 3057717
references.citation: Vet Rec. 1986 Jun 28;118(26):718-21
references.pmid: 3526702
references.citation: Zoonoses Public Health. 2007;54(2):86-93
references.pmid: 17348912
references.citation: Can J Vet Res. 1999 Apr;63(2):101-6
references.pmid: 10369566
references.citation: Res Vet Sci. 2006 Oct;81(2):190-210
references.pmid: 16513150
references.citation: J Vet Diagn Invest. 2005 May;17(3):232-8
references.pmid: 15945378
references.citation: Aust Vet J. 1992 Jan;69(1):1-4
references.pmid: 1554334