title: Unique organizational and functional features of the cytochrome c maturation system in Shewanella oneidensis.
abstract: Shewanella are renowned for their ability to respire on a wide range of electron acceptors, which has been partially accredited to the presence of a large number of the c-type cytochromes. In the model species S. oneidensis MR-1, at least 41 genes encode c-type cytochromes that are predicted to be intact, thereby likely functional. Previously, in-frame deletion mutants for 36 of these genes were obtained and characterized. In this study, first we completed the construction of an entire set of c-type cytochrome mutants utilizing a newly developed att-based mutagenesis approach, which is more effective and efficient than the approach used previously by circumventing the conventional cloning. Second, we investigated the cytochrome c maturation (Ccm) system in S. oneidensis. There are two loci predicted to encode components of the Ccm system, SO0259-SO0269 and SO0476-SO0478. The former is proven essential for cytochrome c maturation whereas the latter is dispensable. Unlike the single operon organization observed in other γ-proteobacteria, genes at the SO0259-SO0269 locus are uniquely organized into four operons, ccmABCDE, scyA, SO0265, and ccmFGH-SO0269. Functional analysis revealed that the SO0265 gene rather than the scyA and SO0269 genes are relevant to cytochrome c maturation.
authors.affiliation: College of Life Sciences and Institute of Microbiology, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Jin
authors.forename: Yaoming
authors.initials: Y
authors.lastname: Jiang
authors.forename: Linlin
authors.initials: L
authors.lastname: Sun
authors.forename: Jianhua
authors.initials: J
authors.lastname: Yin
authors.forename: Huihui
authors.initials: H
authors.lastname: Fu
authors.forename: Genfu
authors.initials: G
authors.lastname: Wu
authors.forename: Haichun
authors.initials: H
authors.lastname: Gao
mesh_terms: D003198:Computer Simulation; D003574:Cytochrome c Group / Q000235:genetics* / Q000378:metabolism*; D004579:Electron Transport; D056426:Genetic Loci / Q000235:genetics; D016296:Mutagenesis; D009154:Mutation; D020592:Shewanella / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D003574:Cytochrome c Group
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