title: Ca2+ clearance mechanisms in cancer cell lines and stromal cells of the prostate.
abstract: BACKGROUND
  Three prostatic cell lines, PC3, LNCaP, and DU 145, are used as established models to study cell signaling in prostate cancer. Recently, stromal cell lines of the prostate, such as P21, were also introduced. Here we investigate a basic and important mechanism of living cells: Ca(2+) homeostasis in PC3, DU 145, and P21.
  
  
  METHODS
  We examined Ca(2+) clearance mechanisms by monitoring the kinetics of recovery from histamine stimulation under conditions which inhibit prospect mechanisms for storing or extrusion of Ca(2+) from the cytosol by photometry.
  
  
  RESULTS
  Despite the fact that in all three cell lines the Ca(2+) ATPase of the plasma membrane and the SERCA are most important for Ca(2+) homeostasis, inhibition of PMCA in epithelial cells has a greater effect than in stromal cells. Furthermore, the proportion of PMCA and SERCA differs in PC3 and DU145 cells. PMCA is most effective at reaching resting [Ca(2+) ]i in the final recovery stage. In contrast to DU 145 and P21 cells, PC3 are the only cells substantially affected by the inhibition of the mitochondrial uniporter. In all cell lines the role of the sodium calcium exchanger is marginal.
  
  
  CONCLUSIONS
  These results demonstrate that not only cancer and stromal cell lines show significant differences in the modes and extent of their use of Ca(2+) clearance mechanisms, but also the cancer cell lines themselves.
authors.affiliation: Department of Anatomy and Cell Biology, Saarland University, Homburg/Saar, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Wolf
authors.forename: Gunther
authors.initials: G
authors.lastname: Wennemuth
mesh_terms: D002118:Calcium / Q000502:physiology*; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D011467:Prostate / Q000166:cytology / Q000502:physiology*; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D017154:Stromal Cells / Q000502:physiology
chemical_list: D002118:Calcium
keywords: Ca2+; calcium; cancer; clearance; prostate