title: Determination of amantadine and rimantadine in chicken muscle by QuEChERS pretreatment method and UHPLC coupled with LTQ Orbitrap mass spectrometry.
abstract: A novel sample pretreatment method was developed for the quantitative determination of amantadine and rimantadine in chicken muscle tissues by ultra high performance liquid chromatography coupled with high resolution LTQ Orbitrap mass spectrometry (UHPLC-LTQ Orbitrap MS). The samples were pretreated by modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method, using acetonitrile (1% acetic acid, v/v) as extraction solution and C18 sorbent for clean-up. The separation was carried out on a Waters ACQUITY UPLC HSS T3 column (150mm×2.1mm, 1.8μm particle), using a mobile phase of acetonitrile and 0.1% aqueous formic acid solution. LTQ Orbitrap MS with resolving power of 60000 was applied for analysis of the samples. Amantadine and rimantadine were identified from their accurate mass (within 5ppm) and retention times from the acquired full-scan chromatogram and quantified by their peak areas. The linear range for the determination of the analytes was 1-100μg/kg. Limits of detection (LODs) for amantadine and rimantadine were 1.02μg/kg and 0.67μg/kg, respectively. The intra- and inter-day accuracy ranged from 87.5% to 102.4%, and 82.5% to 105.8% for amantadine, and 95.3% to 97.4%, and 89.4% to 93.2% for rimantadine, respectively. The precision of intra- and inter-day was between 3.9-6.3% and 5.95-13.9% for amantadine, 6.0-7.45% and 7.8-12.4% for rimantadine, respectively. Finally, the method was applied for the determination of these antiviral agents in routine samples, and amantadine residue was detected in some cases.
authors.affiliation: Food Laboratory of Beijing Inspection and Quarantine Testing Center, Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yan
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Fengyun
authors.initials: F
authors.lastname: Cui
authors.forename: Huan
authors.initials: H
authors.lastname: Yun
authors.forename: Jianhui
authors.initials: J
authors.lastname: Li
authors.forename: Shuangyang
authors.initials: S
authors.lastname: Ding
authors.forename: Dajin
authors.initials: D
authors.lastname: Yang
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000547:Amantadine / Q000032:analysis*; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004350:Drug Residues / Q000032:analysis*; D016018:Least-Squares Analysis; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry*; D015203:Reproducibility of Results; D012299:Rimantadine / Q000032:analysis*
chemical_list: D012299:Rimantadine; D000547:Amantadine
keywords: Amantadine; Chicken muscle tissues; LTQ Orbitrap MS; QuEChERS method; Rimantadine; UHPLC