title: Cloning of TPS gene from eelgrass species Zostera marina and its functional identification by genetic transformation in rice.
abstract: The full-length cDNA sequence (2613 bp) of the trehalose-6-phosphate synthase (TPS) gene of eelgrass Zostera marina (ZmTPS) was identified and cloned. Z. marina is a kind of seed-plant growing in sea water during its whole life history. The open reading frame (ORF) region of ZmTPS gene encodes a protein of 870 amino acid residues and a stop codon. The corresponding genomic DNA sequence is 3770 bp in length, which contains 3 exons and 2 introns. The ZmTPS gene was transformed into rice variety ZH11 via Agrobacterium-mediated transformation method. After antibiotic screening, molecular characterization, salt-tolerance and trehalose content determinations, two transgenic lines resistant to 150 mM NaCL solutions were screened. Our study results indicated that the ZmTPS gene was integrated into the genomic DNA of the two transgenic rice lines and could be expressed well. Moreover, the detection of the transformed ZmTPS gene in the progenies of the two transgenic lines was performed from T1 to T4 generations; and results suggested that the transformed ZmTPS gene can be transmitted from parent to the progeny in transgenic rice.
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.forename: Qiuying
authors.initials: Q
authors.lastname: Li
authors.forename: Manli
authors.initials: M
authors.lastname: Weng
authors.forename: Xiuliang
authors.initials: X
authors.lastname: Wang
authors.forename: Baotai
authors.initials: B
authors.lastname: Guo
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Delin
authors.initials: D
authors.lastname: Duan
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004553:Electric Conductivity; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D030821:Plants, Genetically Modified; D055049:Salt Tolerance / Q000235:genetics; D014170:Transformation, Genetic / Q000502:physiology; D014199:Trehalose / Q000378:metabolism; D035962:Zosteraceae / Q000201:enzymology / Q000235:genetics*
chemical_list: D014199:Trehalose; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase
keywords: Agrobacterium-mediated transformation; EB; EC; Electrical conductivity; Ethidium bromide; GT; Guanidinium thiocyanate; ORF; Open reading frame; RACE; REC; RT-PCR; Rapid amplification of cDNA ends; Relative electrical conductivity; Reverse transcription-polymerase chain reaction; Salt-tolerance; TPS; Transgenic rice; Trehalose-6-phosphate synthase; Trehalose-6-phosphate synthase (TPS); Trehalose-6-phosphate synthase gene of Zostera marina; ZmTPS; Zostera marina