title: Standardization of resistance exercise training: effects in diabetic ovariectomized rats.
abstract: This study was carried out with a 3-fold aim: 1) to standardize a maximal load test (MLT) on ladders for prescription of resistance exercise training (RET) in rats, 2) to prescribe moderate-intensity RET based on this MLT and 3) to test the effect of this RET in diabetic ovariectomized rats. Female Wistar rats were divided into control (C), diabetic ovariectomized sedentary (DOS) and trained (DOT) groups. The MLT was standardized with increased load applied to the rat tail for each climb, and blood lactate was measured to identify lactate threshold in C rats. MLT was applied in the 1st, 4th and 8th week of the protocol. After 8 weeks of RET, the arterial pressure was directly recorded. DOS group reduced performance in MLT, body weight, left ventricular, plantar and soleus muscles mass (vs. C). DOT rats showed an improvement in MLT associated with plantar muscle mass increased (vs. C and DOS), with attenuation of hypotension and bradycardia (vs. DOS). In conclusion, the results provide a useful method for determining the maximal load and applying RET in rats. Moreover, this study showed that moderate intensity RET improves hemodynamic status in diabetic ovariectomized rats, thereby reinforcing the role of RET in diabetes management.
authors.affiliation: Laboratory of Translational Physiology, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil.
authors.forename: I C
authors.initials: IC
authors.lastname: Sanches
authors.affiliation: Laboratory of Translational Physiology, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil.
authors.forename: F F
authors.initials: FF
authors.lastname: Conti
authors.affiliation: Hypertension Unit, Heart Institute (InCor), School of Medicine, University of São Paulo, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Sartori
authors.affiliation: Hypertension Unit, Heart Institute (InCor), School of Medicine, University of São Paulo, Brazil.
authors.forename: M C
authors.initials: MC
authors.lastname: Irigoyen
authors.affiliation: Laboratory of Translational Physiology, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil.
authors.forename: K
authors.initials: K
authors.lastname: De Angelis
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure; D001835:Body Weight; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D005260:Female; D005632:Fructose / Q000008:administration & dosage; D006339:Heart Rate; D019344:Lactic Acid / Q000097:blood; D010052:Ovariectomy; D010805:Physical Conditioning, Animal / Q000379:methods* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D055070:Resistance Training / Q000379:methods*; D013549:Sweetening Agents / Q000008:administration & dosage; D016474:Weight-Bearing
chemical_list: D001786:Blood Glucose; D013549:Sweetening Agents; D005632:Fructose; D019344:Lactic Acid