title: Novel codon insert in HIV type 1 clade B reverse transcriptase associated with low-level viremia during antiretroviral therapy.
abstract: We investigated the pol genotype in two phylogenetically and epidemiologically linked partners, who were both experiencing persistent low-level viremia during antiretroviral therapy. In one partner we identified a new residue insertion between codon 248 and 249 of the HIV-1 RNA reverse transcriptase (RT) coding region (HXB2 numbering). We then investigated the potential impact of identified mutations in RT and antiretroviral binding affinity using a novel computational approach.
authors.affiliation: 1 University of California , San Diego, La Jolla, California.
authors.forename: Antoine
authors.initials: A
authors.lastname: Chaillon
authors.forename: Sara
authors.initials: S
authors.lastname: Gianella
authors.forename: Homero
authors.initials: H
authors.lastname: Vazquez
authors.forename: Caroline
authors.initials: C
authors.lastname: Ignacio
authors.forename: Adam C
authors.initials: AC
authors.lastname: Zweig
authors.forename: Douglas D
authors.initials: DD
authors.lastname: Richman
authors.forename: Davey M
authors.initials: DM
authors.lastname: Smith
mesh_terms: D000595:Amino Acid Sequence; D044966:Anti-Retroviral Agents / Q000627:therapeutic use*; D015658:HIV Infections / Q000188:drug therapy* / Q000821:virology*; D054303:HIV Reverse Transcriptase / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D011487:Protein Conformation; D014766:Viremia
chemical_list: D044966:Anti-Retroviral Agents; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase
references.citation: JAMA. 2008 Aug 6;300(5):555-70
references.pmid: 18677028
references.citation: Chem Biol Drug Des. 2012 Apr;79(4):530-4
references.pmid: 22188672
references.citation: J Gen Virol. 2007 Jan;88(Pt 1):226-235
references.pmid: 17170455
references.citation: Proteins. 2007 Jun 1;67(4):1167-78
references.pmid: 17380508
references.citation: J Comput Chem. 2011 Mar;32(4):742-55
references.pmid: 20812323
references.citation: AIDS Res Hum Retroviruses. 2005 Apr;21(4):311-3
references.pmid: 15943574
references.citation: AIDS. 2005 Mar 24;19(6):549-54
references.pmid: 15802972
references.citation: Clin Infect Dis. 2008 Jul 15;47(2):266-85
references.pmid: 18549313
references.citation: AIDS Res Hum Retroviruses. 2009 May;25(5):547-50
references.pmid: 19397401
references.citation: J Mol Biol. 2000 Aug 18;301(3):691-711
references.pmid: 10966778
references.citation: J Mol Biol. 2007 Oct 26;373(3):573-86
references.pmid: 17869270
references.citation: J Virol. 2008 Jun;82(12):5869-78
references.pmid: 18400858
references.citation: Mol Biol Evol. 2011 Oct;28(10):2731-9
references.pmid: 21546353
references.citation: PLoS One. 2012;7(5):e36673
references.pmid: 22590588
references.citation: Nat Rev Drug Discov. 2004 Nov;3(11):935-49
references.pmid: 15520816