title: P53 protein in proliferation, repair and apoptosis of cells.
abstract: The p53 protein is an important factor of many intra- and extracellular processes. This protein regulates the repair of cellular DNA and induces apoptosis. It is also responsible for the regulation of the senescence and the cell entering the subsequent stages of the cellular cycle. The protein p53 is also involved in inhibiting angiogenesis and the induction of oxidative shock. In our study, we examined the activity of p53 protein in the uterine epithelial cells in rats treated with cladribine. Its action is mainly based on apoptosis induction. We compared the activity of p53 protein in cells with a high apoptosis index and in cells with active repair mechanisms and high proliferation index. We observed stronger p53 protein expression in the epithelial cells of the materials taken 24 h after the last dose of 2-CdA associated with the active process of apoptosis and inhibition of proliferation. After 4 weeks from the last dose of cladribine, the stronger expression of p53 protein was associated with both the existing changes in the cell's genome, the effects of the ongoing repair mechanisms, as well as the high proliferation activity.
authors.affiliation: Department of Histology and Embryology with Experimental Cytology Unit, Medical University in Lublin, ul. Radziwiłłowska 11, 20-080, Lublin, Poland, ewelina.wawryk@wp.pl.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Wawryk-Gawda
authors.forename: Patrycja
authors.initials: P
authors.lastname: Chylińska-Wrzos
authors.forename: Marta
authors.initials: M
authors.lastname: Lis-Sochocka
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Chłapek
authors.forename: Kamila
authors.initials: K
authors.lastname: Bulak
authors.forename: Marian
authors.initials: M
authors.lastname: Jędrych
authors.forename: Barbara
authors.initials: B
authors.lastname: Jodłowska-Jędrych
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D017338:Cladribine / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D005260:Female; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D016159:Tumor Suppressor Protein p53 / Q000502:physiology*; D015854:Up-Regulation; D014599:Uterus / Q000166:cytology / Q000196:embryology
chemical_list: D016159:Tumor Suppressor Protein p53; D017338:Cladribine
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