title: [Expression of vascular growth factors in intestinal tissues in colorectal carcinoma patients with schistosomiasis japonica].
abstract: OBJECTIVE
  To investigate the differences of mRNA quantitation and protein expression of vascular growth factors including platelet-derived endothelial cell growth factor (PD-ECGF) and vascular endothelial growth factor (VEGF) in intestinal tissues in colorectal carcinoma patients with and without schistosomiasis.
  
  
  METHODS
  Thirty colorectal carcinoma patients with schistosomiasis and 30 colorectal carcinoma patients without schistosomiasis were included in this study. The mRNA quantitation and protein expression of PD-ECGF and VEGF in the normal tissue, peri-carcinoma tissue as well as carcinoma tissue obtained from surgical specimens were detected by qRT-PCR and Western blot.
  
  
  RESULTS
  The mRNA relative quantitations of PD-ECGF in normal tissue, peri-carcinoma tissue and carcinoma tissue in the colorectal carcinoma patients with schistosomiasis were 1.726, 1.766 and 2.729 times to those in the colorectal carcinoma patients without schistosomiasis, respectively. The corresponding ones of VEGF were 2.138, 1.831 and 3.376 times, respectively. The protein expression levels of PD-ECGF and VEGF in normal tissue, peri-carcinoma tissue and carcinoma tissue were higher in the colorectal carcinoma patients with schistosomiasis than in the colorectal carcinoma patients without schistosomiasis.
  
  
  CONCLUSIONS
  The expressions of vascular growth factors including PD-ECGF and VEGF are higher in the colorectal carcinoma patients with schistosomiasis than in the colorectal carcinoma patients without schistosomiasis. Therefore, schistosomiasis may be one of the risk factors of colorectal cancer.
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital of Jiaxing College, Jiaxing 314000, China.
authors.forename: Shui-Liang
authors.initials: SL
authors.lastname: Ruan
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Qi-Ming
authors.initials: QM
authors.lastname: Lu
authors.forename: Lai-Rong
authors.initials: LR
authors.lastname: Dong
authors.forename: Chen-Xi
authors.initials: CX
authors.lastname: Cao
authors.forename: Shui-Ling
authors.initials: SL
authors.lastname: Xu
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: Shen
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000469:parasitology; D005260:Female; D015870:Gene Expression; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012549:Schistosoma japonicum / Q000302:isolation & purification / Q000502:physiology; D012554:Schistosomiasis japonica / Q000235:genetics* / Q000378:metabolism / Q000469:parasitology; D013939:Thymidine Phosphorylase / Q000235:genetics* / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000378:metabolism
chemical_list: D042461:Vascular Endothelial Growth Factor A; D013939:Thymidine Phosphorylase