title: Dynein recruitment to nuclear pores activates apical nuclear migration and mitotic entry in brain progenitor cells.
abstract: Radial glial progenitors (RGPs) are elongated epithelial cells that give rise to neurons, glia, and adult stem cells during brain development. RGP nuclei migrate basally during G1, apically using cytoplasmic dynein during G2, and undergo mitosis at the ventricular surface. By live imaging of in utero electroporated rat brain, we find that two distinct G2-specific mechanisms for dynein nuclear pore recruitment are essential for apical nuclear migration. The "RanBP2-BicD2" and "Nup133-CENP-F" pathways act sequentially, with Nup133 or CENP-F RNAi arresting nuclei close to the ventricular surface in a premitotic state. Forced targeting of dynein to the nuclear envelope rescues nuclear migration and cell-cycle progression, demonstrating that apical nuclear migration is not simply correlated with cell-cycle progression from G2 to mitosis, but rather, is a required event. These results reveal that cell-cycle control of apical nuclear migration occurs by motor protein recruitment and identify a role for nucleus- and centrosome-associated forces in mitotic entry. PAPERCLIP:
authors.affiliation: Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, USA.
authors.forename: Daniel Jun-Kit
authors.initials: DJ
authors.lastname: Hu
authors.forename: Alexandre Dominique
authors.initials: AD
authors.lastname: Baffet
authors.forename: Tania
authors.initials: T
authors.lastname: Nayak
authors.forename: Anna
authors.initials: A
authors.lastname: Akhmanova
authors.forename: Valérie
authors.initials: V
authors.lastname: Doye
authors.forename: Richard Bert
authors.initials: RB
authors.lastname: Vallee
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology*; D002352:Carrier Proteins / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D018385:Centrosome / Q000378:metabolism; D004398:Dyneins / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008869:Microtubule-Associated Proteins; D008938:Mitosis; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D055495:Neurogenesis; D022022:Nuclear Pore / Q000378:metabolism*; D051381:Rats
chemical_list: C584496:Bicd2 protein, rat; D002352:Carrier Proteins; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D004398:Dyneins
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