title: Denaturing reversed phase liquid chromatographic separation of non-coding ribonucleic acids on macro-porous polystyrene-divinylbenzene resins.
abstract: The ability of denaturing ion-paired reversed phase LC to separate RNA was assessed using macro-porous polystyrene-divinylbenzene resins as the stationary phase. Using the three stationary phases with different pore size and a mobile phase containing phosphate, we separated RNAs of 20-8000 nucleotides with extremely high sensitivity, e.g., 50pg for an RNA 20 nucleotides in length, S/N=5. The method was used to separate non-coding RNAs obtained from biological sources and is suited for use with direct MS-based chemical characterization.
authors.affiliation: Department of Chemistry, Graduate School of Sciences and Engineering, Tokyo Metropolitan University, 1-1 Minamiosawa, Hachioji-shi, Tokyo 192-0397, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Yamauchi
authors.forename: Masato
authors.initials: M
authors.lastname: Taoka
authors.forename: Yuko
authors.initials: Y
authors.lastname: Nobe
authors.forename: Keiichi
authors.initials: K
authors.lastname: Izumikawa
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Takahashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakayama
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Isobe
mesh_terms: D001483:Base Sequence; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation / Q000379:methods*; D057809:HEK293 Cells; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011137:Polystyrenes / Q000737:chemistry*; D022661:RNA, Untranslated / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D012680:Sensitivity and Specificity
chemical_list: D011137:Polystyrenes; D022661:RNA, Untranslated; C432006:divinylbenzene-polystyrene copolymer
keywords: Denaturing reversed phase high-performance liquid chromatography; Macro-porous polystyrene-divinylbenzene resin; Mass spectrometry; RNA