title: Detection of Group B Streptococcus in Brazilian pregnant women and antimicrobial susceptibility patterns.
abstract: Group B Streptococcus (GBS) is still not routinely screened during pregnancy in Brazil, being prophylaxis and empirical treatment based on identification of risk groups. This study aimed to investigate GBS prevalence in Brazilian pregnant women by culture or polymerase chain reaction (PCR) associated to the enrichment culture, and to determine the antimicrobial susceptibility patterns of isolated bacteria, so as to support public health policies and empirical prophylaxis. After an epidemiological survey, vaginal and anorectal specimens were collected from 221 consenting laboring women. Each sample was submitted to enrichment culture and sheep blood agar was used to isolate suggestive GBS. Alternatively, specific PCR was performed from enrichment cultures. Antimicrobial susceptibility patterns were determined for isolated bacteria by agar diffusion method. No risk groups were identified. Considering the culture-based methodology, GBS was detected in 9.5% of the donors. Twenty five bacterial strains were isolated and identified. Through the culture-PCR methodology, GBS was detected in 32.6% specimens. Bacterial resistance was not detected against ampicillin, cephazolin, vancomycin and ciprofloxacin, whereas 22.7% were resistant to erythromycin and 50% were resistant to clindamycin. GBS detection may be improved by the association of PCR and enrichment culture. Considering that colony selection in agar plates may be laboring and technician-dependent, it may not reflect the real prevalence of streptococci. As in Brazil prevention strategies to reduce the GBS associated diseases have not been adopted, prospective studies are needed to anchor public health policies especially considering the regional GBS antimicrobial susceptibility patterns.
authors.affiliation: Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora , Juiz de Fora, MG , Brasil.
authors.forename: Didier Silveira
authors.initials: DS
authors.lastname: Castellano-Filho
authors.forename: Vânia Lúcia
authors.initials: VL
authors.lastname: da Silva
authors.forename: Thiago César
authors.initials: TC
authors.lastname: Nascimento
authors.forename: Marcel
authors.initials: M
authors.lastname: de Toledo Vieira
authors.forename: Cláudio Galuppo
authors.initials: CG
authors.lastname: Diniz
keywords: Perinatal disease; antimicrobial drug susceptibility; group B streptococci
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