title: High-throughput functional microRNA profiling using recombinant AAV-based microRNA sensor arrays.
abstract: There is a lack of methods for high-throughput functional microRNA (miRNA) profiling. In this chapter, we describe a recombinant adeno-associated virus-based miRNA sensor array (miRNA Asensor array), which is able to profile functional miRNAs in cultured cells. The preparation of an miRNA Asensor array and its usage are discussed.
authors.affiliation: College of Life Science, Jilin University, Changchun, Jilin, China.
authors.forename: Wenhong
authors.initials: W
authors.lastname: Tian
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Dong
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Wu
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000818:Animals; D001483:Base Sequence; D015374:Biosensing Techniques; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D000229:Dependovirus / Q000235:genetics*; D004926:Escherichia coli; D005822:Genetic Vectors; D057166:High-Throughput Screening Assays; D035683:MicroRNAs / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D035683:MicroRNAs
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