title: Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections.
abstract: Pathogenic strains of Escherichia coli are the most common bacteria associated with urinary tract infections in both humans and companion animals. Standard biochemical tests may be useful in demonstrating detailed phenotypical characteristics of these strains. Thirteen strains of E. coli isolated from dogs with UTIs were submitted to biochemical tests, serotyping for O and H antigens and antimicrobial resistance testing. Furthermore, the presence of papC, sfa, and afa genes was evaluated by PCR, and genetic relationships were established using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The antimicrobial that showed the highest resistance rate among the isolates was nalidixic acid (76.9%), followed by cephalotin (69.2%), sulfamethoxazole + trimethoprim (61.5%), tetracycline (61.5%), streptomycin (53.8%), ciprofloxacin (53.8%), ampicillin (46.2%), gentamicin (30.8%) and chloramphenicol (23.1%). No isolate was resistant either to meropenem or nitrofurantoin. Among the five clusters that were identified using ERIC-PCR, one cluster (A) had only one strain, which belonged to a serotype with zoonotic potential (O6:H31) and showed the genes papC+, sfa+, afa-. Strains with the genes papC-, sfa+, afa- were found in two other clusters (C and D), whereas all strains in clusters B and E possessed papC-, sfa-, afa- genes. Sucrose and raffinose phenotypic tests showed some ability in discriminating clusters A, B and C from clusters D and E.
authors.affiliation: Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista , Jaboticabal, SP , Brasil.
authors.forename: Renato Pariz
authors.initials: RP
authors.lastname: Maluta
authors.forename: Ariel Eurides
authors.initials: AE
authors.lastname: Stella
authors.forename: Kátia
authors.initials: K
authors.lastname: Riccardi
authors.forename: Everlon Cid
authors.initials: EC
authors.lastname: Rigobelo
authors.forename: José Moacir
authors.initials: JM
authors.lastname: Marin
authors.forename: Marileda Bonafim
authors.initials: MB
authors.lastname: Carvalho
authors.forename: Fernando Antonio
authors.initials: FA
authors.lastname: de Ávila
keywords: ERIC-PCR; UPEC; biomarkers; biotyping
references.citation: J Clin Microbiol. 2003 Jan;41(1):337-45
references.pmid: 12517870
references.citation: J Infect Dis. 2001 Mar 15;183(6):897-906
references.pmid: 11237806
references.citation: J Gen Intern Med. 2002 Aug;17(8):646-9
references.pmid: 12213147
references.citation: Nucleic Acids Res. 1991 Dec 25;19(24):6823-31
references.pmid: 1762913
references.citation: Appl Environ Microbiol. 1983 Jul;46(1):165-70
references.pmid: 6351743
references.citation: Int J Antimicrob Agents. 2003 Oct;22 Suppl 2:29-33
references.pmid: 14527768
references.citation: Rev Soc Bras Med Trop. 2006 Nov-Dec;39(6):573-6
references.pmid: 17308707
references.citation: Res Vet Sci. 2009 Apr;86(2):206-10
references.pmid: 18783807
references.citation: J Clin Microbiol. 2008 Feb;46(2):417-22
references.pmid: 18003805
references.citation: FEMS Microbiol Lett. 2005 Nov 15;252(2):183-90
references.pmid: 16165319
references.citation: Vet Microbiol. 2001 Sep 3;82(1):81-9
references.pmid: 11423198
references.citation: Braz J Microbiol. 2008 Jul;39(3):477-83
references.pmid: 24031249
references.citation: J Clin Pathol. 1982 Dec;35(12):1366-9
references.pmid: 6757274
references.citation: J Vet Med Sci. 1998 Mar;60(3):287-90
references.pmid: 9560773
references.citation: Infect Immun. 1982 May;36(2):462-8
references.pmid: 6123483
references.citation: Lancet Infect Dis. 2008 Mar;8(3):159-66
references.pmid: 18291338
references.citation: J Clin Microbiol. 1992 May;30(5):1189-93
references.pmid: 1349900
references.citation: Lett Appl Microbiol. 2003;37(2):121-6
references.pmid: 12859653
references.citation: Microb Pathog. 1992 Aug;13(2):161-6
references.pmid: 1360613
references.citation: Vet Microbiol. 2002 Nov 6;89(4):323-8
references.pmid: 12383641
references.citation: J Med Microbiol. 1979 Nov;12(4):473-86
references.pmid: 390154