title: Cyclodextrin glycosyltransferase production by new Bacillus sp. strains isolated from brazilian soil.
abstract: Three strains of Bacillus sp. (BACRP, BACNC-1 and BACAR) were isolated from soil adhered to cassava husk. CGTase specific activity for the three isolated strains was higher when cultivated at 40°C. Potato starch, cassava starch, maltodextrin and glucose were used as carbon source and growth temperatures varied from 25 to 55°C. The three isolates presented higher CGTase specific activity when cultivated with potato starch at 40°C. Isolated BACRP and BACAR presented specific activity of 4.0×10(-3) and 2.2×10(-3) U/mg prot at pH 7.0, respectively, when cultivated in mediums added with NaCl 2%; at pH 10,0 their activities were of 3.4×10(-3) and 3.0×10(-3) U/mg prot, respectively, in the same concentration of NaCl. On the other hand, the isolated BACNC-1 presented activity specific of 2.4×10(-3) U/mg prot when cultivated at pH 7.0 added of NaCl 1%, and at pH 10.0 the specific activity was of 3.4×10(-3) U/mg prot without NaCl addition. This work also showed the presence of cyclodextrins formed during fermentation process and that precipitation with acetone or lyophilization followed by dialysis was efficient at removing CDs (cyclodextrins), thus, eliminating interference in the activity assays. The enzyme produced by the BACAR strain was partially purified and β-CD was liberated as a reaction product.
authors.affiliation: Departamento de Alimentos e Nutrição, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista , Araraquara, SP , Brasil.
authors.forename: Vivian
authors.initials: V
authors.lastname: Menocci
authors.forename: Antonio José
authors.initials: AJ
authors.lastname: Goulart
authors.forename: Paulo Roberto
authors.initials: PR
authors.lastname: Adalberto
authors.forename: Olga Luisa
authors.initials: OL
authors.lastname: Tavano
authors.forename: Daniela Parreira
authors.initials: DP
authors.lastname: Marques
authors.forename: Jonas
authors.initials: J
authors.lastname: Contiero
authors.forename: Rubens
authors.initials: R
authors.lastname: Monti
keywords: Bacillus; CGTase production; cyclodextrin glycosyltransferase
references.citation: Biotechnol Appl Biochem. 1992 Feb;15(1):48-58
references.pmid: 1532314
references.citation: Appl Environ Microbiol. 1999 May;65(5):1991-7
references.pmid: 10223990
references.citation: Appl Environ Microbiol. 1988 Dec;54(12):3156-61
references.pmid: 16347804
references.citation: Ann N Y Acad Sci. 1964 Dec 28;121:404-27
references.pmid: 14240539
references.citation: Microb Cell Fact. 2002 Sep 12;1(1):3
references.pmid: 12392599
references.citation: Chem Rev. 1998 Jul 30;98(5):1743-1754
references.pmid: 11848947
references.citation: Chem Rev. 2006 Mar;106(3):782-817
references.pmid: 16522009
references.citation: Crit Rev Food Sci Nutr. 1993;33(6):501-47
references.pmid: 8216812
references.citation: Chem Rev. 2003 Nov;103(11):4147-73
references.pmid: 14611261
references.citation: J Biochem Biophys Methods. 1987 Apr;14(2):85-92
references.pmid: 3693795
references.citation: Anal Biochem. 1989 Aug 15;181(1):6-11
references.pmid: 2530917
references.citation: Biochim Biophys Acta. 2000 Dec 29;1543(2):336-360
references.pmid: 11150613
references.citation: Chem Rev. 1998 Jul 30;98(5):1741-1742
references.pmid: 11848946
references.citation: Anal Biochem. 1972 Aug;48(2):422-7
references.pmid: 4115981
references.citation: Appl Biochem Biotechnol. 1998 Spring;70-72:267-75
references.pmid: 18575996