title: Genetic variation of Taenia pisiformis collected from Sichuan, China, based on the mitochondrial cytochrome B gene.
abstract: Taenia pisiformis is one of the most important parasites of canines and rabbits. T. pisiformis cysticercus (the larval stage) causes severe damage to rabbit breeding, which results in huge economic losses. In this study, the genetic variation of T. pisiformis was determined in Sichuan Province, China. Fragments of the mitochondrial cytochrome b (cytb) (922 bp) gene were amplified in 53 isolates from 8 regions of T. pisiformis. Overall, 12 haplotypes were found in these 53 cytb sequences. Molecular genetic variations showed 98.4% genetic variation derived from intra-region. FST and Nm values suggested that 53 isolates were not genetically differentiated and had low levels of genetic diversity. Neutrality indices of the cytb sequences showed the evolution of T. pisiformis followed a neutral mode. Phylogenetic analysis revealed no correlation between phylogeny and geographic distribution. These findings indicate that 53 isolates of T. pisiformis keep a low genetic variation, which provide useful knowledge for monitoring changes in parasite populations for future control strategies.
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Deying
authors.initials: D
authors.lastname: Yang
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Ren
authors.forename: Yan
authors.initials: Y
authors.lastname: Fu
authors.forename: Yue
authors.initials: Y
authors.lastname: Xie
authors.forename: Huaming
authors.initials: H
authors.lastname: Nie
authors.forename: Xiang
authors.initials: X
authors.lastname: Nong
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Gu
authors.forename: Shuxian
authors.initials: S
authors.lastname: Wang
authors.forename: Xuerong
authors.initials: X
authors.lastname: Peng
authors.forename: Guangyou
authors.initials: G
authors.lastname: Yang
mesh_terms: D000818:Animals; D002681:China; D045303:Cytochromes b / Q000235:genetics*; D014644:Genetic Variation; D015801:Helminth Proteins / Q000235:genetics*; D006801:Humans; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011817:Rabbits; D013621:Taenia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D013622:Taeniasis / Q000469:parasitology*
chemical_list: D015801:Helminth Proteins; D045303:Cytochromes b
keywords: China; Taenia pisiformis; cytochrome b; genetic variation
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