title: Evaluation of biomass production, carotenoid level and antioxidant capacity produced by Thermus filiformis Using fractional factorial design.
abstract: A fractional factorial design 2(5-1) was used to evaluate the effect of temperature, pH, and concentrations of yeast extract, tryptone and Nitsch's trace elements on the biomass, total carotenoids and protection against singlet oxygen by carotenoid extracts of the bacterium Thermus filiformis. In addition, the carotenoid composition was determined by high-performance liquid chromatography connected to a diode array and mass spectrometer detectors (HPLC-DAD-MS/MS). The production of biomass ranged from 0.113 to 0.658 g/L, the total carotenoid from 137.6 to 1,517.4 µg/g and the protection against singlet oxygen from 4.3 to 85.1 %. Results of the fractional factorial design showed that temperature had a negative effect on biomass production and a positive effect on carotenoid content and protection against singlet oxygen, besides, high levels of pH value, concentrations of yeast extract and tryptone had a positive effect on biomass production only at lower temperatures. The main carotenoids of T. filiformis were thermozeaxanthins. In the tested conditions, changes in the levels of the variables influenced the biomass, carotenoid production, and protection against singlet oxygen, although they did not influence the carotenoid profile. The results of this study provide a better understanding on the interactions among certain nutritional and cultivation conditions of a thermophile bacterium, Thermus filiformis, on biomass and carotenoid amounts, as well as on the antioxidant capacity.
authors.affiliation: Departamento de Ciência de Alimentos, Faculdade de Engenharia de Alimentos, Universidade de Campinas , Campinas, SP , Brasil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Mandelli
authors.forename: Fábio
authors.initials: F
authors.lastname: Yamashita
authors.forename: José L
authors.initials: JL
authors.lastname: Pereira
authors.forename: Adriana Z
authors.initials: AZ
authors.lastname: Mercadante
keywords: HPLC-DAD-MS/MS; antioxidant capacity; carotenoids; factorial design; thermophile bacterium
references.citation: Nature. 2001 Feb 22;409(6823):1092-101
references.pmid: 11234023
references.citation: J Chem Technol Biotechnol. 1992;53(4):313-27
references.pmid: 1368195
references.citation: J Agric Food Chem. 2007 Oct 17;55(21):8516-22
references.pmid: 17894451
references.citation: J Bacteriol. 1969 Apr;98(1):289-97
references.pmid: 5781580
references.citation: J Agric Food Chem. 2004 Jan 28;52(2):367-73
references.pmid: 14733523
references.citation: FEMS Microbiol Lett. 2004 Aug 1;237(1):111-8
references.pmid: 15268945
references.citation: J Agric Food Chem. 2007 Jun 27;55(13):5062-72
references.pmid: 17530774
references.citation: Appl Biochem Biotechnol. 1997 Spring;63-65:305-14
references.pmid: 18576089
references.citation: Glycobiology. 2006 Aug;16(8):766-75
references.pmid: 16636007
references.citation: FEBS Lett. 2000 Nov 10;484(3):280-4
references.pmid: 11078893
references.citation: Prikl Biokhim Mikrobiol. 2010 Jan-Feb;46(1):5-20
references.pmid: 20198911
references.citation: Bioresour Technol. 2005 Feb;96(3):373-8
references.pmid: 15474940
references.citation: Ageing Res Rev. 2009 Jul;8(3):199-213
references.pmid: 19427410
references.citation: Prikl Biokhim Mikrobiol. 1994 Mar-Apr;30(2):181-95
references.pmid: 8183855