title: [Establishment and clinical application of TapMan real-time RT-PCR method for detection of HHV-6].
abstract: OBJECTIVE
  To establish a rapid, sensitive and specific real-time PCR method for detection of Human Herpesvirus-6 (HHV-6).
  
  
  METHODS
  According to the reference, a pair of primers and a probe were designed located in U65-66 gene and to set up the standards. We established a real-time RT-PCR method for detection of HHV-6, and to verify the specificity, sensitivity, reproducibility.
  
  
  RESULTS
  The correlation coefficient was 0.999, E = 97.9%, the coefficient of variation values of Ct were 0.61% and 3.13% in real-time PCR assay for inter and intra assay, respectively. The results of all viruses were negative except of HHV-6 for the assay. The quantitative detection limit of the assay was 3 x 10(0) copies/microl.
  
  
  CONCLUSIONS
  The real-time PCR assay is highly specific, sensitive and reproducible, which can be used to quatitative detecting clinical samples.
authors.affiliation: University of South China, Hengyang 421000, China.
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Chen
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Xie
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Li
authors.forename: Han-Chun
authors.initials: HC
authors.lastname: Gao
authors.forename: Ni-Guang
authors.initials: NG
authors.lastname: Xiao
authors.forename: Le-Yun
authors.initials: LY
authors.lastname: Xie
authors.forename: Tian
authors.initials: T
authors.lastname: Yu
authors.forename: Sai-Zhen
authors.initials: SZ
authors.lastname: Zeng
authors.forename: Ping
authors.initials: P
authors.lastname: Gong
authors.forename: Zhao-Jun
authors.initials: ZJ
authors.lastname: Duan
mesh_terms: D015654:Herpesvirus 6, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*