title: Hepatitis B virus core protein interacts with CD59 to promote complement-mediated liver inflammation during chronic hepatitis B virus infection.
abstract: The inflammatory response mediated by the immune system is the major cause of hepatitis B virus (HBV)-associated liver injury. Here, we identified CD59, as a novel HBc-interacting protein in hepatocytes by tandem affinity purification (TAP) screening. The expression of CD59 was markedly down-regulated in HBc-transfected HepG2 or HepG2.215 cells, which resulted in an upshift of hepatocyte sensitivity to membrane attack complex (MAC)-induced cell lysis. These results were consistent with the accumulation of MACs in the liver of HBV-infected patients. Additional analyses using laser confocal microscopy, quantitative PCR and flow cytometry revealed that CD59 was specifically translocated to the nucleus upon binding to HBc, which induced the down-regulation of CD59 on both the mRNA and protein levels.
authors.affiliation: Institute of Immunology, PLA, The Third Military Medical University, Chongqing 400038, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.forename: Bing
authors.initials: B
authors.lastname: Ni
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Mengjun
authors.initials: M
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Yuzhang
authors.initials: Y
authors.lastname: Wu
mesh_terms: D015153:Blotting, Western; D018957:CD59 Antigens / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D056945:Hep G2 Cells; D006515:Hepatitis B virus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D006521:Hepatitis, Chronic / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D018613:Microscopy, Confocal; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D018957:CD59 Antigens; D015678:Viral Structural Proteins
keywords: CD59; Complement; HBV; HBc; Inflammation; MAC