title: Characterization of recombinant MSP5 Anaplasma marginale Havana isolate.
abstract: Anaplasma marginale is the causative agent of bovine anaplasmosis, a disease of worldwide economic importance. Major surface proteins (MSPs) are involved in host-pathogen and tick-pathogen interactions and they have been used as markers for the genetic characterization of A. marginale strains and phylogenetic studies. The major surface protein 5 (MSP5) is highly conserved in the genus Anaplasma and in all isolates of A. marginale. The aim of the present work was to carry out the cloning, sequencing and characterization of the recombinant MSP5 Anaplasma marginale Havana isolate. The sequence of the msp5 gene of Anaplasma marginale Havana isolate with a size of 633 pb was determined (Acc. No. AY527217). This gene was cloned into pRSETB vector and expressed in Escherichia coli. The MSP5 protein was recognized by the monoclonal antibody ANAF16C1 and it showed a high similitude percent with the gene sequence described for other Anaplasma marginale isolates. These data are very important for the development of a diagnostic test for A. marginale using the MSP5 recombinant protein.
authors.affiliation: National Centre for Animal and Plant Health. Apartado 10, postal address 32700, San José de las Lajas , La Habana , Cuba.
authors.forename: B
authors.initials: B
authors.lastname: Corona
authors.forename: H
authors.initials: H
authors.lastname: Machado
authors.forename: M
authors.initials: M
authors.lastname: Rodríguez
authors.forename: S
authors.initials: S
authors.lastname: Martínez
keywords: Anaplasma marginale; HMSP5; Havana isolate; characterization; major surface proteins
references.citation: J Clin Microbiol. 2001 Feb;39(2):631-5
references.pmid: 11158120
references.citation: Clin Diagn Lab Immunol. 1998 Mar;5(2):259-62
references.pmid: 9521155
references.citation: Infect Immun. 1997 Jan;65(1):156-63
references.pmid: 8975906
references.citation: Onderstepoort J Vet Res. 1987 Mar;54(1):63-5
references.pmid: 3587929
references.citation: Vet Microbiol. 2002 Jun 20;87(2):131-8
references.pmid: 12034541
references.citation: Vet Microbiol. 2004 May 20;100(1-2):55-64
references.pmid: 15135513
references.citation: Infect Immun. 1994 Sep;62(9):3808-16
references.pmid: 8063397
references.citation: Int J Parasitol. 1988 Feb;18(1):33-8
references.pmid: 2452797
references.citation: Infect Immun. 2002 Nov;70(11):6005-12
references.pmid: 12379676
references.citation: Biotechniques. 1990 Sep;9(3):352-7
references.pmid: 2223077
references.citation: Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8
references.pmid: 3162770
references.citation: Infect Immun. 1992 Dec;60(12):5139-44
references.pmid: 1280624
references.citation: Vet Parasitol. 1995 Mar;57(1-3):233-53
references.pmid: 7597787
references.citation: Infect Immun. 2000 Apr;68(4):1946-52
references.pmid: 10722587
references.citation: Parasitol Today. 1999 Jul;15(7):281-6
references.pmid: 10377531
references.citation: Onderstepoort J Vet Res. 1987 Dec;54(4):623-7
references.pmid: 2832800
references.citation: J Clin Microbiol. 1992 Jun;30(6):1374-9
references.pmid: 1624551
references.citation: Gene. 2001 Sep 19;275(2):287-98
references.pmid: 11587856
references.citation: J Clin Microbiol. 1995 Mar;33(3):675-9
references.pmid: 7538510
references.citation: Vet Clin Pathol. 2006 Dec;35(4):418-25
references.pmid: 17123248
references.citation: Ann N Y Acad Sci. 1996 Jul 23;791:157-65
references.pmid: 8784497
references.citation: Clin Vaccine Immunol. 2007 Mar;14(3):262-8
references.pmid: 17215333
references.citation: Infect Immun. 1999 Jan;67(1):102-7
references.pmid: 9864202
references.citation: Zentralbl Veterinarmed B. 1992 Jun;39(4):246-52
references.pmid: 1519404
references.citation: J Clin Microbiol. 1991 Apr;29(4):788-93
references.pmid: 1890178
references.citation: Infect Immun. 1998 Mar;66(3):1200-7
references.pmid: 9488414
references.citation: Infect Immun. 1994 Jul;62(7):2940-6
references.pmid: 8005681
references.citation: Infect Immun. 1998 Jun;66(6):2619-24
references.pmid: 9596725
references.citation: J Clin Microbiol. 1998 Mar;36(3):777-82
references.pmid: 9508311
references.citation: J Clin Microbiol. 1996 Sep;34(9):2225-30
references.pmid: 8862589