title: Evaluation of INNO-LiPA mycobacteria v2 assay for identification of rapidly growing mycobacteria.
abstract: A total of 54 rapidly growing mycobacteria (RGM) isolated from patients attended in the two hospitals of Cádiz Bay (Spain) were selected during a seven-year-period (2000-2006) in order to evaluate the INNO-LiPA Mycobacteria v2 assay for mycobacterial identification, based on the reverse hybridization principle. The strains were cultured in Löwenstein-Jensen and Middlebrook 7H9 media and identified to the species level by sequencing of the 16S rRNA, PCR-restriction enzyme analysis of the hsp65 gene, conventional tests and INNO-LiPA Mycobacteria v2 assay. By the molecular methods we identified a total of 12 different species: 23 Mycobacterium fortuitum, 11 M. chelonae, 10 M. abscessus, 2 M. senegalense, 1 M. alvei, 1 M. brumae, 1 M. mageritense, 1 M. mucogenicum, 1 M. neoaurum, 1 M. peregrinum, 1 M. septicum and 1 M. smegmatis. Fifty two strains (96.3%) were correctly identified by conventional techniques and 47 strains (87.0%) by INNO-LiPA Mycobacteria v2 assay. We find INNO-LiPA Mycobacteria v2 assay simple to perform but it provides few advantages in comparison with conventional methods and sometimes needs complementary tests to identify Mycobacterium fortuitum complex, M. chelonae complex and specific species due to the great heterogeneity in the RGM group.
authors.affiliation: Microbiology Service. Hospital de Puerto Real , Carretera Nacional IV km 665, 11510 Puerto Real, Cádiz , Spain.
authors.forename: Lidia
authors.initials: L
authors.lastname: García-Agudo
authors.forename: Iría
authors.initials: I
authors.lastname: Jesús
authors.forename: Manuel
authors.initials: M
authors.lastname: Rodríguez-Iglesias
authors.forename: Pedro
authors.initials: P
authors.lastname: García-Martos
keywords: M. abscessus; M. chelonae; M. fortuitum; Mycobacteria; rapidly growing mycobacteria
references.citation: J Clin Microbiol. 2004 Jul;42(7):3083-8
references.pmid: 15243064
references.citation: J Clin Microbiol. 2001 Aug;39(8):2799-806
references.pmid: 11473995
references.citation: J Clin Microbiol. 1995 Dec;33(12):3225-33
references.pmid: 8586707
references.citation: Pathol Biol (Paris). 2004 Oct;52(8):462-8
references.pmid: 15465265
references.citation: J Clin Microbiol. 2005 Jun;43(6):2567-74
references.pmid: 15956365
references.citation: Annu Rev Microbiol. 1998;52:453-90
references.pmid: 9891805
references.citation: Arch Dermatol. 2006 Oct;142(10):1287-92
references.pmid: 17043183
references.citation: J Clin Microbiol. 2002 Sep;40(9):3478-81
references.pmid: 12202597
references.citation: J Infect. 2007 Apr;54(4):e219-21
references.pmid: 17316814
references.citation: J Clin Microbiol. 2003 Sep;41(9):4418-20
references.pmid: 12958281
references.citation: Braz J Microbiol. 2008 Oct;39(4):613-8
references.pmid: 24031276
references.citation: Int J Syst Evol Microbiol. 2004 Sep;54(Pt 5):1653-1667
references.pmid: 15388725
references.citation: Clin Microbiol Rev. 2003 Apr;16(2):319-54
references.pmid: 12692101
references.citation: Clin Infect Dis. 2002 Jun 1;34(11):1500-7
references.pmid: 12015697
references.citation: J Clin Microbiol. 2001 Dec;39(12):4477-82
references.pmid: 11724865
references.citation: Clin Infect Dis. 2006 Jun 15;42(12):1756-63
references.pmid: 16705584
references.citation: Microbiology (Reading). 1995 Oct;141 ( Pt 10):2739-47
references.pmid: 7582034
references.citation: Braz J Microbiol. 2010 Apr;41(2):386-90
references.pmid: 24031509
references.citation: J Clin Microbiol. 1993 Feb;31(2):175-8
references.pmid: 8381805