title: Characterization and comparison of serratia marcescens isolated from edible cactus and from silkworm for virulence potential and chitosan susceptibility.
abstract: Representative strains of Serratia marcescens from an edible cactus plant and silkworms were characterized and a comparison based on their cellular fatty acid composition, 16S rRNA and groE gene sequence analysis as well as silkworm virulence and chitosan susceptibility was carried out. Results from this study indicate that there are no significant differences between the phenotypic and molecular characterization, virulence and chitosan susceptibility of the S. marcescens strains from the cactus plant and silkworms. Silkworms inoculated with S. marcescens from either plant or silkworm resulted in nearly 100% mortality. Chitosan solution exhibited strong antibacterial activity against S. marcescens. This activity increased with the increase of chitosan concentration and incubation time regardless of the strain source. Also, the results indicate that the plant associated S. marcescens maybe plays a possible role in the contamination of humans and animals, in particular silkworms, while chitosan showed a potential to control the contamination caused by S. marcescens.
authors.affiliation: State Key Laboratory of Rice Biology, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology, Zhejiang University , Hangzhou 310029 , China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Rongrong
authors.initials: R
authors.lastname: Yu
authors.forename: Baoping
authors.initials: B
authors.lastname: Liu
authors.forename: Qiaomei
authors.initials: Q
authors.lastname: Tang
authors.forename: Guoqing
authors.initials: G
authors.lastname: Zhang
authors.forename: Yanli
authors.initials: Y
authors.lastname: Wang
authors.forename: Guanlin
authors.initials: G
authors.lastname: Xie
authors.forename: Guochang
authors.initials: G
authors.lastname: Sun
keywords: Cactus; Characterization; Chitosan susceptibility; Serratia marcescens; Silkworm; Virulence
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