title: Molecular identification of arbuscular mycorrhizal fungal spores collected in Korea.
abstract: Arbuscular mycorrhizas (AM) have mutualistic symbiosis with plants and thus efforts have been placed on application of these symbiotic relationships to agricultural and environmental fields. In this study, AM fungi were collected from 25 sites growing with 16 host plant species in Korea and cultured with Sorghum bicolor in greenhouse condition. AM fungal spores were extracted and identified using both morphological and molecular methods. Using morphological characters, total 15 morpho-speices were identified. DNA was extracted from single spore of AM fungi and a partial region on 18S rDNA was amplified using nested PCR with AM fungal specific primers AML1/AML2. A total of 36 18S rDNA sequences were analyzed for phylogenetic analysis and 15 groups of AM fungi were identified using both morphological and molecular data of spores. Among the species, 4 species, Archaeospora leptoticha, Scutellospora castanea, S. cerradensis, S. weresubiae were described for the first time in Korea and two species in Glomus and a species in Gigaspora were not identified. Morphological and molecular identification of AM fungal spores in this study would help identify AM fungal community colonizing roots.
authors.affiliation: Department of Biology Education, Korea National University of Education, Chungbuk 363-791, Republic of Korea.
authors.forename: Jaikoo
authors.initials: J
authors.lastname: Lee
authors.forename: Sang-Hyeon
authors.initials: SH
authors.lastname: Park
authors.forename: Ahn-Heum
authors.initials: AH
authors.lastname: Eom
keywords: 18S rDNA; Arbuscular mycorrhiza; Specific primer
references.citation: Nature. 2003 Oct 23;425(6960):798-804
references.pmid: 14574403
references.citation: Mol Biol Evol. 1987 Jul;4(4):406-25
references.pmid: 3447015
references.citation: Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
references.pmid: 7984417
references.citation: Mol Ecol. 1998 Jul;7(7):879-87
references.pmid: 9691489
references.citation: Appl Environ Microbiol. 1997 May;63(5):1756-61
references.pmid: 16535592