title: Immune response to dna vaccine expressing transferrin binding protein a gene of Pasteurella multocida.
abstract: Haemorrhagic Septicaemia (HS), an acute and fatal disease of cattle and buffalo is primarily caused by serotype B:2 or E:2 of Pasteurella multocida. The transferrin binding protein A (TbpA) has been found to act as immunogen and potent vaccine candidate in various Gram negative bacteria including P. multocida. The present study was carried out to evaluate the potential of this antigen as a DNA vaccine against HS in mice model. The tbpA gene of P. multocida serotype B:2 was cloned in a mammalian expression vector alone and along with murine IL2 gene as immunological adjuvant to produce monocistronic and bicistronic DNA vaccine constructs, respectively. The immune response to DNA vaccines was evaluated based on serum antibody titres and lymphocyte proliferation assay. A significant increase in humoral and cell mediated immune responses was observed in mice vaccinated with DNA vaccines as compared to non immunized group. Additionally, the bicistronic DNA vaccine provided superior immune response and protection level following challenge as compared to monocistronic construct. The study revealed that DNA vaccine presents a promising approach for the prevention of HS.
authors.affiliation: Division of Bacteriology & Mycology, Indian Veterinary Research Institute , Izatnagar-243122 , India.
authors.forename: Satparkash
authors.initials: S
authors.lastname: Singh
authors.forename: Vijendra Pal
authors.initials: VP
authors.lastname: Singh
authors.forename: Pawanjit Singh
authors.initials: PS
authors.lastname: Cheema
authors.forename: Maninder
authors.initials: M
authors.lastname: Sandey
authors.forename: Rajeev
authors.initials: R
authors.lastname: Ranjan
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Gupta
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Sharma
keywords: DNA vaccine; Haemorrhagic septicaemia; IL2; Pasteurella multocida; Transferrin binding protein
references.citation: Vaccine. 2006 May 22;24(21):4471-4
references.pmid: 16140432
references.citation: J Bacteriol. 2001 Feb;183(3):890-6
references.pmid: 11208786
references.citation: Immunol Lett. 2008 May 15;117(2):136-45
references.pmid: 18339431
references.citation: Vaccine. 2007 Jan 4;25(2):253-63
references.pmid: 16971027
references.citation: Mol Microbiol. 1996 Feb;19(3):575-86
references.pmid: 8830248
references.citation: J Virol. 1997 Jan;71(1):169-78
references.pmid: 8985336
references.citation: Res Vet Sci. 1976 Jan;20(1):90-6
references.pmid: 1257631
references.citation: Immunobiology. 2006;211(1-2):65-74
references.pmid: 16446171
references.citation: Infect Immun. 2001 Jun;69(6):4174-6
references.pmid: 11349095
references.citation: Infect Immun. 2003 Mar;71(3):1155-60
references.pmid: 12595427
references.citation: J Chin Med Assoc. 2006 Jan;69(1):7-13
references.pmid: 16447920
references.citation: Cell Mol Life Sci. 1999 May;55(5):751-70
references.pmid: 10379361
references.citation: Virology. 2004 Nov 10;329(1):89-100
references.pmid: 15476877
references.citation: Vaccine. 2007 May 22;25(21):4140-8
references.pmid: 17449151
references.citation: Am J Vet Res. 2001 May;62(5):697-703
references.pmid: 11341388
references.citation: Jpn J Pharmacol. 1997 Oct;75(2):199-201
references.pmid: 9414037
references.citation: Microb Pathog. 1996 Jul;21(1):59-64
references.pmid: 8827707
references.citation: Vaccine. 2008 Apr 16;26(17):2100-10
references.pmid: 18378365
references.citation: Avian Dis. 2001 Jul-Sep;45(3):572-80
references.pmid: 11569729
references.citation: Vaccine. 2007 Nov 23;25(48):8163-71
references.pmid: 17959278
references.citation: Infect Immun. 2004 Apr;72(4):2081-7
references.pmid: 15039330
references.citation: Vaccine. 1996 Jan;14(1):49-53
references.pmid: 8821649
references.citation: Adv Immunol. 1993;54:229-70
references.pmid: 8379463
references.citation: Infect Immun. 2002 Jul;70(7):3355-62
references.pmid: 12065473
references.citation: J Intern Med. 2003 Apr;253(4):402-10
references.pmid: 12653868
references.citation: Science. 1996 Jul 19;273(5273):352-4
references.pmid: 8662521
references.citation: Microb Pathog. 1999 Oct;27(4):197-206
references.pmid: 10502460
references.citation: Infect Immun. 2005 Mar;73(3):1475-81
references.pmid: 15731045
references.citation: World J Gastroenterol. 2007 Feb 14;13(6):939-44
references.pmid: 17352028
references.citation: Infect Immun. 2003 Sep;71(9):4901-7
references.pmid: 12933831
references.citation: Vaccine. 2005 Apr 27;23(23):2994-3004
references.pmid: 15811645
references.citation: Infect Immun. 2001 Mar;69(3):1561-7
references.pmid: 11179327
references.citation: Res Vet Sci. 2004 Aug;77(1):49-57
references.pmid: 15120952