title: Proinflammatory signal transduction pathway induced by Shigella flexneri porins in caco-2 cells.
abstract: The recognition of bacterial components on the intestinal epithelial cells occurs through the toll-like receptors and is followed by the induction of an effective innate immune response. We analyzed receptor expression and signaling pathways involved in activation of human colon adenocarcinoma cells after stimulation with porins and LPS of Shigella flexneri. We also analyzed the expression and production of some cytokines, of intercellular adhesion molecule-1, of antimicrobial peptides human β-defensins, and of the inducible form of nitric oxide synthase. Our data demonstrate that TLR2 is involved in porin recognition, whereas TLR4 with MD2, is required for LPS recognition.
authors.affiliation: Department of Experimental Medicine, Microbiology and Clinical Microbiology Section, Faculty of Medicine and Surgery, Second University of Naples , 80138 Naples , Italy.
authors.forename: Grimaldi
authors.initials: G
authors.lastname: Elena
authors.forename: Donnarumma
authors.initials: D
authors.lastname: Giovanna
authors.forename: Perfetto
authors.initials: P
authors.lastname: Brunella
authors.forename: Filippis
authors.initials: F
authors.lastname: De Anna
authors.forename: Melito
authors.initials: M
authors.lastname: Alessandro
authors.forename: Tufano Maria
authors.initials: TM
authors.lastname: Antonietta
keywords: PAMPs; Porins; Shigella flexneri; TLRs
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