title: Isolation of human adipose-derived stromal cells using laser-assisted liposuction and their therapeutic potential in regenerative medicine.
abstract: Harvesting adipose-derived stromal cells (ASCs) for tissue engineering is frequently done through liposuction. However, several different techniques exist. Although third-generation ultrasound-assisted liposuction has been shown to not have a negative effect on ASCs, the impact of laser-assisted liposuction on the quality and differentiation potential of ASCs has not been studied. Therefore, ASCs were harvested from laser-assisted lipoaspirate and suction-assisted lipoaspirate. Next, in vitro parameters of cell yield, cell viability and proliferation, surface marker phenotype, osteogenic differentiation, and adipogenic differentiation were performed. Finally, in vivo bone formation was assessed using a critical-sized cranial defect in athymic nude mice. Although ASCs isolated from suction-assisted lipoaspirate and laser-assisted lipoaspirate both successfully underwent osteogenic and adipogenic differentiation, the cell yield, viability, proliferation, and frequency of ASCs (CD34(+)CD31(-)CD45(-)) in the stromal vascular fraction were all significantly less with laser-assisted liposuction in vitro (p < .05). In vivo, quantification of osseous healing by micro-computed tomography revealed significantly more healing with ASCs isolated from suction-assisted lipoaspirate relative to laser-assisted lipoaspirate at the 4-, 6-, and 8-week time points (p < .05). Therefore, as laser-assisted liposuction appears to negatively impact the biology of ASCs, cell harvest using suction-assisted liposuction is preferable for tissue-engineering purposes.
authors.affiliation: Hagey Laboratory for Pediatric Regenerative Medicine, Plastic and Reconstructive Surgery Division, Department of Surgery, and.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Chung
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Zimmermann
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Paik
authors.forename: Shane D
authors.initials: SD
authors.lastname: Morrison
authors.forename: Jeong S
authors.initials: JS
authors.lastname: Hyun
authors.forename: David D
authors.initials: DD
authors.lastname: Lo
authors.forename: Adrian
authors.initials: A
authors.lastname: McArdle
authors.forename: Daniel T
authors.initials: DT
authors.lastname: Montoro
authors.forename: Graham G
authors.initials: GG
authors.lastname: Walmsley
authors.forename: Kshemendra
authors.initials: K
authors.lastname: Senarath-Yapa
authors.forename: Michael
authors.initials: M
authors.lastname: Sorkin
authors.forename: Robert
authors.initials: R
authors.lastname: Rennert
authors.forename: Hsin-Han
authors.initials: HH
authors.lastname: Chen
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Chung
authors.forename: Dean
authors.initials: D
authors.lastname: Vistnes
authors.forename: Geoffrey C
authors.initials: GC
authors.lastname: Gurtner
authors.forename: Michael T
authors.initials: MT
authors.lastname: Longaker
authors.forename: Derrick C
authors.initials: DC
authors.lastname: Wan
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D005434:Flow Cytometry; D006801:Humans; D007834:Lasers; D015187:Lipectomy / Q000379:methods*; D051379:Mice; D060888:Real-Time Polymerase Chain Reaction; D044968:Regenerative Medicine / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*
keywords: Adipose; Adult stem cells; Laser lipoplasty; Liposuction; Stem cell transplantation; Stromal cells
references.citation: Endocrinology. 2010 Nov;151(11):5247-54
references.pmid: 20844002
references.citation: Plast Reconstr Surg. 2010 Sep;126(3):822-834
references.pmid: 20811215
references.citation: Cytotherapy. 2006;8(2):166-77
references.pmid: 16698690
references.citation: Plast Reconstr Surg. 2006 Sep;118(3 Suppl):108S-120S
references.pmid: 16936550
references.citation: Expert Rev Med Devices. 2009 Nov;6(6):621-40
references.pmid: 19911874
references.citation: Stem Cells Dev. 2011 Feb;20(2):233-42
references.pmid: 20528145
references.citation: Aesthet Surg J. 2007 May-Jun;27(3):263-8
references.pmid: 19341652
references.citation: Plast Reconstr Surg. 2009 Jul;124(1):65-73
references.pmid: 19568046
references.citation: Semin Cutan Med Surg. 2008 Dec;27(4):259-63
references.pmid: 19150297
references.citation: Clin Plast Surg. 2006 Jan;33(1):107-15, vii
references.pmid: 16427979
references.citation: Clin Dermatol. 1992 Jul-Sep;10(3):365-72
references.pmid: 1483255
references.citation: Nat Biotechnol. 2004 May;22(5):560-7
references.pmid: 15077117
references.citation: J Craniofac Surg. 2011 Mar;22(2):388-91
references.pmid: 21415625
references.citation: Nucleic Acids Res. 2003 Dec 15;31(24):e154
references.pmid: 14654707
references.citation: Stem Cells Dev. 2009 Oct;18(8):1201-10
references.pmid: 19226222
references.citation: Regen Med. 2009 Jan;4(1):109-17
references.pmid: 19105620
references.citation: Ann Plast Surg. 2008 May;60(5):538-44
references.pmid: 18434829
references.citation: J Plast Reconstr Aesthet Surg. 2011 Aug;64(8):985-92
references.pmid: 21168378
references.citation: Mol Ther. 2009 Feb;17(2):302-8
references.pmid: 19066595
references.citation: Plast Reconstr Surg. 2011 Aug;128(2):373-386
references.pmid: 21788829
references.citation: Cytotherapy. 2004;6(1):7-14
references.pmid: 14985162
references.citation: J Cell Physiol. 2006 Jul;208(1):64-76
references.pmid: 16557516
references.citation: Ultrasonics. 1990 Sep;28(5):280-90
references.pmid: 2203195
references.citation: J Mol Endocrinol. 2009 Feb;42(2):139-47
references.pmid: 18996960
references.citation: Tissue Eng. 2006 Dec;12(12):3375-82
references.pmid: 17518674
references.citation: Int J Urol. 2010 Jan;17(1):75-82
references.pmid: 20002225
references.citation: Methods Mol Biol. 2011;698:201-14
references.pmid: 21431521
references.citation: Science. 2001 Jul 13;293(5528):211-2
references.pmid: 11452974
references.citation: Ultrasound Med Biol. 2001 Jun;27(6):851-9
references.pmid: 11516545
references.citation: Clin Plast Surg. 1996 Oct;23(4):575-98
references.pmid: 8906391
references.citation: Endocrine. 2009 Jun;35(3):402-8
references.pmid: 19277908
references.citation: Biomaterials. 2010 Jul;31(19):5122-30
references.pmid: 20347134
references.citation: Stem Cells Dev. 2007 Feb;16(1):91-104
references.pmid: 17348807