title: Expression of integrin β3 and osteopontin in the eutopic endometrium of adenomyosis during the implantation window.
abstract: OBJECTIVE
  To investigate the expression of integrin β3 and osteopontin (OPN) in the eutopic endometrium of adenomyosis and to characterize possible endometrial defects in adenomyosis.
  
  
  METHODS
  Twenty-eight adenomyosis patients and 27 control fertile women were involved in this study. Endometrial samples were collected during the implantation window. Integrin β3 subunits and OPN in the adenomyosis and control endometria were assessed by immunohistochemical staining and quantitative real-time polymerase chain reaction. A one-tailed t-test and a non-parametric Mann-Whitney U-test were used to test significance.
  
  
  RESULTS
  The mRNA and immunostaining intensity of integrin β3 and OPN were significantly lower in the adenomyosis patients than in the controls.
  
  
  CONCLUSIONS
  Abnormal expression of integrin β3 and OPN in the endometrium of adenomyosis may contribute to infertility in some patients.
authors.affiliation: Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing 100038, People's Republic of China; Department of Obstetrics and Gynaecology, Jessop Wing, Sheffield Teaching Hospitals and Sheffield Hallam University, Sheffield, S10 2SF, United Kingdom; Hysterosopic Center, Fuxing Hospital, Capital Medical University, Beijing 100038, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiao
authors.forename: Tinchiu
authors.initials: T
authors.lastname: Li
authors.forename: Enlan
authors.initials: E
authors.lastname: Xia
authors.forename: Xiuli
authors.initials: X
authors.lastname: Yang
authors.forename: Xiao
authors.initials: X
authors.lastname: Sun
authors.forename: Yingfang
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D062788:Adenomyosis / Q000378:metabolism*; D000328:Adult; D016022:Case-Control Studies; D010064:Embryo Implantation; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000209:etiology; D039661:Integrin beta3 / Q000378:metabolism*; D053495:Osteopontin / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D039661:Integrin beta3; D012333:RNA, Messenger; D053495:Osteopontin
keywords: Adenomyosis; Endometriosis; Implantation window; Integrin β3; Osteopontin