title: Effects of magnetic nanoparticle-incorporated human bone marrow-derived mesenchymal stem cells exposed to pulsed electromagnetic fields on injured rat spinal cord.
abstract: Transplanting mesenchymal stem cells into injured lesions is currently under study as a therapeutic approach for spinal cord injury. In this study, the effects of a pulsed electromagnetic field (PEMF) on injured rat spinal cord were investigated in magnetic nanoparticle (MNP)-incorporated human bone marrow-derived mesenchymal stem cells (hBM-MSCs). A histological analysis revealed significant differences in MNP-incorporated cell distribution near the injured site under the PEMF in comparison with that in the control group. We confirmed that MNP-incorporated cells were widely distributed in the lesions under PEMF. The results suggest that MNP-incorporated hBM-MSCs were guided by the PEMF near the injured site, and that PEMF exposure for 8 H per day over 4 weeks promoted behavioral recovery in spinal cord injured rats. The results show that rats with MNP-incorporated hBM-MSCs under a PEMF were more effective on the Basso, Beattie, and Bresnahan behavioral test and suggest that the PEMF enhanced the action of transplanted cells for recovery of the injured lesion.
authors.affiliation: Research Institute of Biotechnology, Dongguk University, Seoul, Korea.
authors.forename: Hyunjin
authors.initials: H
authors.lastname: Cho
authors.forename: Yun-Kyong
authors.initials: YK
authors.lastname: Choi
authors.forename: Dong Heon
authors.initials: DH
authors.lastname: Lee
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: Park
authors.forename: Young-Kwon
authors.initials: YK
authors.lastname: Seo
authors.forename: Hyun
authors.initials: H
authors.lastname: Jung
authors.forename: Soo-Chan
authors.initials: SC
authors.lastname: Kim
authors.forename: Sung-Min
authors.initials: SM
authors.lastname: Kim
authors.forename: Jung-Keug
authors.initials: JK
authors.lastname: Park
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D001854:Bone Marrow Cells / Q000166:cytology*; D004574:Electromagnetic Fields; D006801:Humans; D058185:Magnetite Nanoparticles; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009416:Nerve Regeneration; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000473:pathology / Q000503:physiopathology / Q000601:surgery
chemical_list: D058185:Magnetite Nanoparticles
keywords: human bone marrow-derived mesenchymal stem cells; magnetic nanoparticle; pulsed electromagnetic field; spinal cord injury