title: Utilization of barley or wheat bran to bioconvert glutamate to γ-aminobutyric acid (GABA).
abstract: This study deals with the utilization of agro-industrial wastes created by barley and wheat bran in the production of a value-added product, γ-aminobutyric acid (GABA). The simple and eco-friendly reaction requires no pretreatment or microbial fermentation steps but uses barley or wheat bran as an enzyme source, glutamate as a substrate, and pyridoxal 5'-phosphate (PLP) as a cofactor. The optimal reaction conditions were determined on the basis of the temperatures and times used for the decarboxylation reactions and the initial concentrations of barley or wheat bran, glutamate, and PLP. The optimal reactions produced 9.2 mM of GABA from 10 mM glutamate, yielding a 92% GABA conversion rate, when barley bran was used and 6.0 mM of GABA from 10 mM glutamate, yielding a 60% GABA conversion rate, when wheat bran was used. The results imply that barley bran is more efficient than wheat bran in the production of GABA.
authors.affiliation: Dept. of Food Science and Technology, Chung-Ang Univ., Ansung, South Korea.
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Jin
authors.forename: Min-Ju
authors.initials: MJ
authors.lastname: Kim
authors.forename: Keun-Sung
authors.initials: KS
authors.lastname: Kim
mesh_terms: D004043:Dietary Fiber / Q000032:analysis*; D005285:Fermentation; D005511:Food Handling; D018698:Glutamic Acid / Q000378:metabolism*; D001467:Hordeum / Q000737:chemistry*; D013696:Temperature; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D004043:Dietary Fiber; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid
keywords: barley bran; decarboxylation; glutamate; wheat bran; γ-aminobutyric acid