title: [Modulation of RhoA/Rho kinase on migration, invasion and proliferation of fibroblast like synoviocytes from patients with rheumatoid arthritis].
abstract: OBJECTIVE
  To evaluate the modulation of RhoA/Rho kinase (ROCK), a small Rho GTPase, on migration, invasion and proliferation of fibroblast like synoviocytes (FLS) from rheumatoid arthritis (RA) patients.
  
  
  METHODS
  RA FLS were collected from active RA patients. And 10% fetal bovine serum (FBS) and interleukin-1β (IL-1β) were used as stimuli in migration and proliferation experiments respectively. RhoA activity was measured by pull down assay while ROCK activity by Western blot. FLS migration and invasion in vitro were measured by the Transwell chamber method. And thiazolyl blue tetrazolium bromide (MTT) test was used to detect cell proliferation.
  
  
  RESULTS
  There were increased activities of RhoA and ROCK in ex vivo FLS from RA versus OA patients and healthy control. The migrated cell number of FBS-induced, C3-treated and Y27632-treated groups was 85 ± 14, 51 ± 15 and 42 ± 11 respectively. The Matrigel invading cell number of 3 groups was 64 ± 13, 39 ± 12 and 26 ± 9 respectively. Statistical differences existed in cell number between FBS-induced, C3-treated or Y27632-treated group (P < 0.05) in above migration and invasion experiments. Inhibition of RhoA and ROCK activity also suppressed the cytoskeletal reorganization and proliferation of RA FLS.
  
  
  CONCLUSIONS
  Increased RhoA/ROCK activity may contribute to abnormal migration, invasion and proliferation of RA FLS. Thus inhibition of ROCK activity may be a new therapeutic target for RA.
authors.affiliation: Department of Rheumatology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Liu-qin
authors.initials: LQ
authors.lastname: Liang
authors.forename: Ming-cheng
authors.initials: MC
authors.lastname: Huang
authors.forename: Qian
authors.initials: Q
authors.lastname: Qiu
authors.forename: You-jun
authors.initials: YJ
authors.lastname: Xiao
authors.forename: Meng-ying
authors.initials: MY
authors.lastname: Sun
authors.forename: Zhong-ping
authors.initials: ZP
authors.lastname: Zhan
authors.forename: Yu-jin
authors.initials: YJ
authors.lastname: Ye
authors.forename: Jin-jin
authors.initials: JJ
authors.lastname: Fan
authors.forename: Xiu-yan
authors.initials: XY
authors.lastname: Yang
authors.forename: Han-shi
authors.initials: HS
authors.lastname: Xu
mesh_terms: D001172:Arthritis, Rheumatoid / Q000378:metabolism* / Q000473:pathology; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008297:Male; D013583:Synovial Membrane / Q000166:cytology; D054460:rho-Associated Kinases / Q000378:metabolism*; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
chemical_list: D054460:rho-Associated Kinases; D020742:rhoA GTP-Binding Protein