title: Chronological changes in the liver after temporary partial portal venous occlusion.
abstract: OBJECTIVE
  To investigate time-dependent changes caused by temporal portal vein obstruction and subsequent reperfusion in the lobe with or without an occluded portal vein.
  
  
  METHODS
  The portal vein (PV) of the anterior lobe of the liver of a male Wistar rat (8 wk-old) was obstructed (70%) for 12, 24, 36 and 48 h, respectively, and models were sacrificed at 48 h after reperfusion (each group: n = 10). The histological changes and the status of liver regeneration were compared between a liver biopsy performed on each lobe after temporary obstruction of the portal vein in the same rat liver, and the liver extracted at the time of sacrifice (48 h after reperfusion).
  
  
  RESULTS
  With regard to the obstructed lobe, the liver weight/body weight ratio significantly decreased according to obstruction time. On the other hand, in the non-obstructed lobe, there were no significant differences within each group. The duration of PV occlusion did not seem to be strong enough to introduce liver weight increase. Stimulation of liver regeneration was brought about in the non-occluded lobe by 12-h occlusion, and was sustained even at 48 h after reperfusion. The obstructed lobe atrophied with the passage of time in the obstructed state. However, the proliferating-cell nuclear antigen labeling index also increased at 48 h after reperfusion, and a repair mechanism was observed.
  
  
  CONCLUSIONS
  Temporary blood flow obstruction of the portal vein may become a significant trigger for liver regeneration, even with an obstruction of 12 h.
authors.affiliation: Koji Hamasaki, Susumu Eguchi, Akihiko Soyama, Masaaki Hidaka, Mitsuhisa Takatsuki, Fumihiko Fujita, Kengo Kanetaka, Shigeki Minami, Tamotsu Kuroki, Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8501, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Hamasaki
authors.forename: Susumu
authors.initials: S
authors.lastname: Eguchi
authors.forename: Akihiko
authors.initials: A
authors.lastname: Soyama
authors.forename: Masaaki
authors.initials: M
authors.lastname: Hidaka
authors.forename: Mitsuhisa
authors.initials: M
authors.lastname: Takatsuki
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Fujita
authors.forename: Kengo
authors.initials: K
authors.lastname: Kanetaka
authors.forename: Shigeki
authors.initials: S
authors.lastname: Minami
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Kuroki
mesh_terms: D000818:Animals; D008115:Liver Regeneration; D008297:Male; D011169:Portal Vein; D051381:Rats; D017208:Rats, Wistar; D060205:Therapeutic Occlusion; D013997:Time Factors
keywords: Liver; Occlusion; Portal vein; Regeneration; Temporary
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