title: Studies on chromatographic fingerprint and fingerprinting profile-efficacy relationship of polygoni perfoliati herba.
abstract: Polygoni Perfoliati Herba is widely used in China with antibacterium, anti-inflammatory, expectorant, antitumor, and antivirus activities. To reveal the mechanisms of the activities of Polygoni Perfoliati Herba, the relationship between the fingerprinting profile and its bioactivities was investigated. In the present study, high-performance liquid chromatographic (HPLC) fingerprinting method was developed. The established method was applied to analyze 51 batches of Polygoni Perfoliati Herba samples collected from different locations or in different harvesting times in China. Chemometrics, including similarity analysis, hierarchical clustering analysis, and principal component analysis, were used to express their similarities. It was found that similarity values of the samples were in the range of 0.432-0.998. The results of analgesic tests indicated that Polygoni Perfoliati Herba could significantly inhibit pain induced by hot plate and acetic acid in mice. The results of anti-inflammatory tests showed that Polygoni Perfoliati Herba had good anti-inflammatory effects (P < 0.01) in two models including dimethyl benzene-induced ear edema and acetic acid-induced peritoneal permeability in mice. Combining the results from chromatographic fingerprints with those from bioactivities, we found that seven peaks from Polygoni Perfoliati Herba were mainly responsible for analgesic and anti-inflammatory activities.
authors.affiliation: The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, China.
authors.forename: Li
authors.initials: L
authors.lastname: Tian
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.forename: Hua-Guo
authors.initials: HG
authors.lastname: Chen
authors.forename: Chao
authors.initials: C
authors.lastname: Zhao
authors.forename: Xiao-Jian
authors.initials: XJ
authors.lastname: Gong
references.citation: J Nat Prod. 1998 Jun 26;61(6):762-6
references.pmid: 9644060
references.citation: Fitoterapia. 2011 Sep;82(6):805-10
references.pmid: 21570451
references.citation: Food Chem. 2011 Jun 1;126(3):1269-1277
references.pmid: 25544798
references.citation: Agents Actions. 1989 Mar;26(3-4):335-41
references.pmid: 2567568
references.citation: J Ethnopharmacol. 2004 Mar;91(1):69-73
references.pmid: 15036471
references.citation: J AOAC Int. 2010 Jul-Aug;93(4):1148-54
references.pmid: 20922946
references.citation: Br J Pharmacol Chemother. 1964 Apr;22:246-53
references.pmid: 14190460
references.citation: Anal Bioanal Chem. 2013 May;405(13):4477-85
references.pmid: 23314619
references.citation: J Nat Prod. 2000 Aug;63(8):1094-7
references.pmid: 10978204
references.citation: Planta Med. 1999 Oct;65(7):671-3
references.pmid: 17260292
references.citation: J Ethnopharmacol. 2000 Sep;72(1-2):273-7
references.pmid: 10967481