title: Kinetic modeling of batch fermentation for Populus hydrolysate tolerant mutant and wild type strains of Clostridium thermocellum.
abstract: The extent of inhibition of two strains of Clostridium thermocellum by a Populus hydrolysate was investigated. A Monod-based model of wild type (WT) and Populus hydrolysate tolerant mutant (PM) strains of the cellulolytic bacterium C. thermocellum was developed to quantify growth kinetics in standard media and the extent of inhibition to a Populus hydrolysate. The PM was characterized by a higher growth rate (μmax=1.223 vs. 0.571 h(-1)) and less inhibition (KI,gen=0.991 vs. 0.757) in 10% v/v Populus hydrolysate compared to the WT. In 17.5% v/v Populus hydrolysate inhibition of PM increased slightly (KI,gen=0.888), whereas the WT was strongly inhibited and did not grow in a reproducible manner. Of the individual inhibitors tested, 4-hydroxybenzoic acid was the most inhibitory, followed by galacturonic acid. The PM did not have a greater ability to detoxify the hydrolysate than the WT.
authors.affiliation: Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, TN, United States; Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United States.
authors.forename: Jessica L
authors.initials: JL
authors.lastname: Linville
authors.affiliation: Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United States; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.
authors.forename: Miguel
authors.initials: M
authors.lastname: Rodriguez
authors.affiliation: Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United States; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Mielenz
authors.affiliation: Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, TN, United States; Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United States; Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN, United States. Electronic address: ccox9@utk.edu.
authors.forename: Chris D
authors.initials: CD
authors.lastname: Cox
mesh_terms: D048013:Acetivibrio thermocellus / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D007700:Kinetics; D032107:Populus / Q000378:metabolism*
keywords: Biofuels; Clostridium thermocellum; Inhibition; Kinetic model; Populus hydrolysate