title: Suppression of cancer-initiating cells and selection of adipose-derived stem cells cultured on biomaterials having specific nanosegments.
abstract: Cancer-initiating cells [cancer stem cells (CSCs)] in colon cancer cells can be selectively suppressed when they are cultured on Pluronic (nanosegment)-grafted dishes, whereas CSCs are maintained on conventional tissue culture dishes and extracellular matrix-coated dishes. CSCs persist in tumors as a distinct population and cause relapse and metastasis by giving rise to new tumorigenic clones. The purification or depletion (suppression) of CSCs should be useful for analyzing CSC characteristics and for clinical application. CSCs can be selectively suppressed from colon cancer cells containing adipose-derived stem cells (ADSCs) on Pluronic-grafted dishes, while ADSCs remain on the dishes. ADSCs on Pluronic-grafted dishes after the suppression of the CSCs can differentiate into osteoblasts, chondrocytes, adipocytes, cardiomyocytes, and neuronal cells. The CSCs and ADSCs exhibited different characteristics. The selection of ADSCs was possible on Pluronic-grafted dishes that suppressed the CSCs from the fat tissues of cancer patients (i.e., cell-sorting dishes), which was explained by specific biomedical characteristics of Pluronic.
authors.affiliation: Department of Chemical and Materials Engineering, National Central University, Jhongli, Taoyuan, 32001, Taiwan.
authors.forename: Ta-Chun
authors.initials: TC
authors.lastname: Kao
authors.forename: Henry Hsin-Chung
authors.initials: HH
authors.lastname: Lee
authors.forename: Akon
authors.initials: A
authors.lastname: Higuchi
authors.forename: Qing-Dong
authors.initials: QD
authors.lastname: Ling
authors.forename: Wan-Chun
authors.initials: WC
authors.lastname: Yu
authors.forename: Yu-Hsuan
authors.initials: YH
authors.lastname: Chou
authors.forename: Pin-Yu
authors.initials: PY
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Suresh Kumar
authors.forename: Yu
authors.initials: Y
authors.lastname: Chang
authors.forename: Yung
authors.initials: Y
authors.lastname: Hung Chen
authors.forename: Yung
authors.initials: Y
authors.lastname: Chang
authors.forename: Da-Chung
authors.initials: DC
authors.lastname: Chen
authors.forename: Shih-Tien
authors.initials: ST
authors.lastname: Hsu
mesh_terms: D017667:Adipocytes / Q000502:physiology*; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001672:Biocompatible Materials; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008875:Middle Aged; D009368:Neoplasm Transplantation; D014411:Neoplastic Stem Cells / Q000502:physiology*; D020442:Poloxamer; D011137:Polystyrenes; D013234:Stem Cells / Q000502:physiology*
chemical_list: D001672:Biocompatible Materials; D011137:Polystyrenes; D020442:Poloxamer
keywords: Pluronic; cell-sorting dish; extracellular matrix; nanosegment; stem cell