title: Inflammatory bowel disease therapies and gut function in a colitis mouse model.
abstract: BACKGROUND
  Exclusive enteral nutrition (EEN) is a well-established approach to the management of Crohn's disease. Aim. To determine effects of EEN upon inflammation and gut barrier function in a colitis mouse model.
  
  
  METHODS
  Interleukin-10-deficient mice (IL-10(-/-)) were inoculated with Helicobacter trogontum and then treated with EEN, metronidazole, hydrocortisone, or EEN and metronidazole combination. Blood and tissue were collected at 2 and 4 weeks with histology, mucosal integrity, tight junction integrity, inflammation, and H. trogontum load evaluated.
  
  
  RESULTS
  H. trogontum induced colitis in IL-10(-/-) mice with histological changes in the cecum and colon. Elevated mucosal IL-8 mRNA in infected mice was associated with intestinal barrier dysfunction indicated by decreased transepithelial electrical resistance and mRNA of tight junction proteins and increased short-circuit current, myosin light chain kinase mRNA, paracellular permeability, and tumor necrosis factor- α and myeloperoxidase plasma levels (P < 0.01 for all comparisons). EEN and metronidazole, but not hydrocortisone, treatments restored barrier function, maintained gut barrier integrity, and reversed inflammatory changes along with reduction of H. trogontum load (versus infected controls P < 0.05).
  
  
  CONCLUSIONS
  H. trogontum infection in IL-10(-/-) mice induced typhlocolitis with intestinal barrier dysfunction. EEN and metronidazole, but not hydrocortisone, modulate barrier dysfunction and reversal of inflammatory changes.
authors.affiliation: School of Women's and Children's Health, University of New South Wales, Randwick, Sydney, NSW 2031, Australia.
authors.forename: Lily
authors.initials: L
authors.lastname: Nahidi
authors.forename: Steven T
authors.initials: ST
authors.lastname: Leach
authors.forename: Hazel M
authors.initials: HM
authors.lastname: Mitchell
authors.forename: Nadeem O
authors.initials: NO
authors.lastname: Kaakoush
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Lemberg
authors.forename: John S
authors.initials: JS
authors.lastname: Munday
authors.forename: Karina
authors.initials: K
authors.lastname: Huinao
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Day
mesh_terms: D000818:Animals; D003092:Colitis / Q000382:microbiology / Q000473:pathology* / Q000628:therapy; D003424:Crohn Disease / Q000382:microbiology / Q000473:pathology* / Q000628:therapy; D004195:Disease Models, Animal; D004750:Enteral Nutrition; D016998:Helicobacter / Q000472:pathogenicity; D006801:Humans; D007249:Inflammation / Q000382:microbiology / Q000473:pathology; D015212:Inflammatory Bowel Diseases / Q000382:microbiology / Q000473:pathology* / Q000628:therapy; D016753:Interleukin-10 / Q000235:genetics* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism; D008795:Metronidazole / Q000008:administration & dosage; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D016753:Interleukin-10; D008795:Metronidazole
references.citation: Am J Physiol Gastrointest Liver Physiol. 2005 Mar;288(3):G422-30
references.pmid: 15701621
references.citation: Infect Immun. 2006 Dec;74(12):6615-23
references.pmid: 16982822
references.citation: Gastroenterology. 1994 Jun;106(6):1455-66
references.pmid: 8194690
references.citation: Appl Environ Microbiol. 2007 Jul;73(13):4259-67
references.pmid: 17496132
references.citation: World J Gastroenterol. 2008 Jan 21;14(3):401-7
references.pmid: 18200662
references.citation: Clin Gastroenterol Hepatol. 2006 Jun;4(6):744-53
references.pmid: 16682258
references.citation: Helicobacter. 2008 Aug;13(4):234-8
references.pmid: 18665930
references.citation: Gastroenterology. 1998 Jul;115(1):182-205
references.pmid: 9649475
references.citation: Aliment Pharmacol Ther. 2001 Nov;15(11):1827-36
references.pmid: 11683697
references.citation: Aliment Pharmacol Ther. 2008 Feb 15;27(4):293-307
references.pmid: 18045244
references.citation: Int J Syst Bacteriol. 1996 Oct;46(4):916-21
references.pmid: 8863417
references.citation: J Biol Chem. 2011 Oct 28;286(43):37578-89
references.pmid: 21880720
references.citation: Scand J Gastroenterol Suppl. 2001;(234):29-40
references.pmid: 11768558
references.citation: Am J Gastroenterol. 1999 Mar;94(3):674-8
references.pmid: 10086650
references.citation: Lett Appl Microbiol. 2008 Apr;46(4):469-76
references.pmid: 18298454
references.citation: Aliment Pharmacol Ther. 2000 Mar;14(3):281-9
references.pmid: 10735920
references.citation: J Clin Microbiol. 2001 Nov;39(11):3920-6
references.pmid: 11682508
references.citation: Am J Physiol Gastrointest Liver Physiol. 2008 Mar;294(3):G669-78
references.pmid: 18187519
references.citation: Ann N Y Acad Sci. 2009 May;1165:301-7
references.pmid: 19538320
references.citation: Am J Pathol. 2005 Feb;166(2):409-19
references.pmid: 15681825
references.citation: Inflamm Bowel Dis. 2006 Jul;12(7):566-72
references.pmid: 16804393
references.citation: Am J Gastroenterol. 2002 Apr;97(4):803-23
references.pmid: 12003413
references.citation: DNA Cell Biol. 2006 Feb;25(2):79-86
references.pmid: 16460231
references.citation: Gastroenterology. 1999 May;116(5):1107-14
references.pmid: 10220502
references.citation: Helicobacter. 2013 Aug;18(4):316-20
references.pmid: 23406293
references.citation: Aliment Pharmacol Ther. 2008 Sep 15;28(6):724-33
references.pmid: 19145728
references.citation: J Clin Microbiol. 2006 Jun;44(6):2276-9
references.pmid: 16757639
references.citation: PLoS One. 2010 Aug 20;5(8):e12207
references.pmid: 20808826
references.citation: Gastroenterology. 2001 Sep;121(3):580-91
references.pmid: 11522742
references.citation: Mucosal Immunol. 2011 Jan;4(1):22-30
references.pmid: 20944559
references.citation: J Med Food. 2011 May;14(5):468-74
references.pmid: 21434779
references.citation: Gastroenterology. 2009 Apr;136(4):1182-97
references.pmid: 19249397
references.citation: Curr Drug Targets. 2012 Sep 1;13(10):1294-9
references.pmid: 22664087
references.citation: Infect Immun. 2011 Sep;79(9):3607-18
references.pmid: 21746860
references.citation: Curr Treat Options Gastroenterol. 2005 Jun;8(3):223-228
references.pmid: 15913511
references.citation: Proc Natl Acad Sci U S A. 2005 Nov 29;102(48):17519-24
references.pmid: 16299103
references.citation: Inflamm Bowel Dis. 2008 Mar;14(3):353-8
references.pmid: 18069674
references.citation: Hear Res. 1999 Nov;137(1-2):167-73
references.pmid: 10545644
references.citation: Intern Med. 2003 Apr;42(4):318-21
references.pmid: 12729319
references.citation: Dig Liver Dis. 2006 Jun;38(6):381-7
references.pmid: 16301010
references.citation: Inflamm Bowel Dis. 2001 Nov;7(4):301-5
references.pmid: 11720319
references.citation: Aliment Pharmacol Ther. 2007 Jan 15;25(2):111-22
references.pmid: 17229236
references.citation: Gastroenterology. 2000 Jun;118(6):1094-105
references.pmid: 10833484
references.citation: Gut. 1991 Sep;32(9):1071-5
references.pmid: 1916494
references.citation: Gut. 2007 Jan;56(1):6-8
references.pmid: 17172583
references.citation: Gut. 2004 Dec;53(12):1724-5
references.pmid: 15542502
references.citation: Infect Immun. 2009 Jun;77(6):2508-16
references.pmid: 19307212
references.citation: Psychoneuroendocrinology. 2008 Oct;33(9):1248-56
references.pmid: 18691825
references.citation: Ann Intern Med. 2001 Jul 3;135(1):27-31
references.pmid: 11434729
references.citation: J Am Coll Nutr. 2004 Jun;23(3):220-6
references.pmid: 15190046
references.citation: J Gastroenterol. 2012 Aug;47(8):872-82
references.pmid: 22699323
references.citation: J Gastroenterol. 2012 Feb;47(2):107-17
references.pmid: 21953313
references.citation: Am J Physiol Gastrointest Liver Physiol. 2008 Jan;294(1):G139-47
references.pmid: 17991705
references.citation: Curr Opin Pharmacol. 2009 Dec;9(6):715-20
references.pmid: 19632896
references.citation: Am J Physiol. 1998 Nov;275(5):G1037-44
references.pmid: 9815034
references.citation: Aliment Pharmacol Ther. 2002 Dec;16(12):1997-2015
references.pmid: 12452933
references.citation: J Leukoc Biol. 2004 Jul;76(1):135-44
references.pmid: 15107456
references.citation: Res Microbiol. 1999 Apr;150(3):205-12
references.pmid: 10229950
references.citation: Am J Physiol Gastrointest Liver Physiol. 2008 Sep;295(3):G452-9
references.pmid: 18635602
references.citation: Inflamm Bowel Dis. 2011 Jan;17(1):382-95
references.pmid: 20645321
references.citation: Curr Drug Targets. 2010 Feb;11(2):227-33
references.pmid: 20210770
references.citation: Med Health R I. 2009 Mar;92(3):78-81
references.pmid: 19385382
references.citation: Am J Physiol. 1999 May;276(5):C1046-52
references.pmid: 10329951
references.citation: Gut. 2007 Jan;56(1):61-72
references.pmid: 16822808
references.citation: Gut. 2007 Nov;56(11):1522-8
references.pmid: 17339238
references.citation: Dig Dis Sci. 2007 Sep;52(9):2029-36
references.pmid: 17406842
references.citation: Aliment Pharmacol Ther. 2001 Nov;15(11):1709-16
references.pmid: 11683684
references.citation: World J Gastroenterol. 2010 Sep 14;16(34):4264-71
references.pmid: 20818809
references.citation: J Mol Neurosci. 2007;31(1):13-21
references.pmid: 17416966
references.citation: Eur Rev Med Pharmacol Sci. 2009 Mar;13 Suppl 1:33-5
references.pmid: 19530509
references.citation: Histochem Cell Biol. 2008 Jul;130(1):55-70
references.pmid: 18415116
references.citation: J R Soc Med. 1998 Mar;91(3):135-7
references.pmid: 9659325