title: Multiplex analyses of cytokine and chemokine release from the cultured fibroblast of nasal polyps: the effect of IL-17A.
abstract: CONCLUSIONS
  Our results demonstrate for the first time a potentially enhanced basal secretion of monocyte chemoattractant protein-1 (MCP-1) and interleukin (IL)-17A-stimulated secretion of IL-6 from nasal polyp fibroblasts, enhanced basal secretion of IL-6 from eosinophilic nasal polyp fibroblasts, and a remarkable up-regulation of IL-9 and granulocyte colony-stimulating factor (G-CSF) from nasal fibroblasts by IL-17A stimulation.
  
  
  OBJECTIVE
  The fibroblast, one of the main cell types making up nasal polyps, is thought to be a target cell of various cytokines.
  
  
  METHODS
  Subcultured fibroblasts were established from human polyp biopsy tissues. Simultaneous quantification of 27 kinds of cytokines and chemokines in culture supernatants in unstimulated and IL-17A-stimulated conditions was performed with a human multiplex cytokine assay system.
  
  
  RESULTS
  The IL-17A receptor was expressed at similar levels in all three groups. In the eosinophilic group, basal secretion levels of IL-6 were significantly higher than those in the control and non-eosinophilic groups. Basal secretion of MCP-1 in both the non-eosinophilic and eosinophilic groups was also higher than that of the control group. Both IL-9 and G-CSF secretion were remarkably enhanced by IL-17A stimulation in all three groups. The receptor-mediated response by IL-17A significantly up-regulated IL-6 release alone in the non-eosinophilic and eosinophilic groups as compared with the control group.
authors.affiliation: Department of Otorhinolaryngology, Juntendo University Faculty of Medicine , Tokyo , Japan.
authors.forename: Hirotomo
authors.initials: H
authors.lastname: Homma
authors.forename: Kazusaku
authors.initials: K
authors.lastname: Kamiya
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kusunoki
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Ikeda
mesh_terms: D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D018925:Chemokines / Q000096:biosynthesis*; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation; D006801:Humans; D007118:Immunoassay; D020381:Interleukin-17 / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D018925:Chemokines; D016207:Cytokines; D020381:Interleukin-17; D012333:RNA, Messenger