title: Facile immunostaining and labeling of nonadherent cells using a microfluidic device to entrap the cells.
abstract: We fabricated a microfluidic device for the entrapment of nonadherent cells. Solution exchange was easily performed by introducing the solution into the cell-trapping microchannel. Immunostaining and labeling of the cell membrane of THP-1 cells were demonstrated using this device, which does not require cumbersome repetition of centrifugation and resuspension steps.
authors.affiliation: Stress Signal Research Group, Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan. Electronic address: hironori-yamazoe@aist.go.jp.
authors.forename: Hironori
authors.initials: H
authors.lastname: Yamazoe
authors.affiliation: Research Institute of Biomolecule Metrology (RIBM), 807-133 Enokido, Tsukuba, Ibaraki 305-0853, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Sugiyama
authors.affiliation: Research Institute of Biomolecule Metrology (RIBM), 807-133 Enokido, Tsukuba, Ibaraki 305-0853, Japan.
authors.forename: Abdelfatteh
authors.initials: A
authors.lastname: El Omri
authors.affiliation: Stress Signal Research Group, Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Hagihara
authors.affiliation: Research Institute of Biomolecule Metrology (RIBM), 807-133 Enokido, Tsukuba, Ibaraki 305-0853, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Okada
mesh_terms: D000199:Actins / Q000378:metabolism; D002448:Cell Adhesion; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009000:Monocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism
chemical_list: D000199:Actins; D005456:Fluorescent Dyes
keywords: Albumin film; Cell membrane labeling; Immunostaining; Microbeads; Microfluidic device; Solution exchange