title: Chitinase Production by Streptomyces sp. ANU 6277.
abstract: Chitinase production by a terrestrial Streptomyces sp. ANU 6277 was studied under sub-merged fermentation. Chitinase production started after 24 h of incubation and reached maximum levels after 60 h of cultivation. A high level of chitinase activity was observed in the culture medium with pH 6 at 35°C. Culture medium amended with 1% chitin was found to be suitable for maximum production of chitinase. An optimum concentration of colloidal chitin for chitinase production was determined. Studies on the influence of additional carbon and nitrogen sources on chitinase production revealed that starch and yeast extract served as good carbon and nitrogen sources to enhance chitinase yield. Chitinase was purified from crude enzyme extract by single step gel filtration by Sephadex G-100. Purified chitinase of the strain exhibited a distinct protein band near 45 kDa by means of SDS-PAGE.
authors.affiliation: Department of Microbiology, Acharya Nagarjuna University , Guntur 522 510, A.P. , India.
authors.forename: Kolla J P
authors.initials: KJ
authors.lastname: Narayana
authors.forename: Muvva
authors.initials: M
authors.lastname: Vijayalakshmi
keywords: Characterization; Chitinase; Optimization; Streptomyces sp. ANU 6277
references.citation: J Biol Chem. 1955 Dec;217(2):959-66
references.pmid: 13271455
references.citation: Appl Microbiol Biotechnol. 2006 Aug;71(6):773-82
references.pmid: 16249876
references.citation: Nature. 1970 Aug 15;227(5259):680-5
references.pmid: 5432063
references.citation: Can J Microbiol. 1998 Jul;44(7):646-51
references.pmid: 9783424
references.citation: J Basic Microbiol. 2004;44(1):49-58
references.pmid: 14768028
references.citation: Phytopathology. 2000 Jan;90(1):72-7
references.pmid: 18944574
references.citation: Pol J Microbiol. 2007;56(3):191-7
references.pmid: 18062653
references.citation: J Biosci Bioeng. 2000;89(1):27-32
references.pmid: 16232694
references.citation: J Biochem Mol Biol. 2003 Mar 31;36(2):185-9
references.pmid: 12689517
references.citation: Indian J Exp Biol. 2004 Jul;42(7):715-20
references.pmid: 15339036
references.citation: J Appl Bacteriol. 1995 Apr;78(4):378-83
references.pmid: 7744723
references.citation: Biotechnol Lett. 2005 Oct;27(19):1483-6
references.pmid: 16231220
references.citation: Crit Rev Biotechnol. 2007 Jan-Mar;27(1):21-8
references.pmid: 17364687
references.citation: J Appl Microbiol. 2001 Apr;90(4):653-61
references.pmid: 11309080
references.citation: J Appl Microbiol. 2001 May;90(5):803-8
references.pmid: 11348442
references.citation: Braz J Microbiol. 2008 Apr;39(2):314-20
references.pmid: 24031223
references.citation: J Appl Microbiol. 2002;93(6):965-75
references.pmid: 12452952
references.citation: Can J Microbiol. 2006 Sep;52(9):805-8
references.pmid: 17110971