title: Lactobacillus acidophilus La5, Bifidobacterium BB12, and Lactobacillus casei DN001 modulate gene expression of subset specific transcription factors and cytokines in peripheral blood mononuclear cells of obese and overweight people.
abstract: Probiotics are believed to have interaction with immune cells through sustained effects on gene expression of different cytokines and transcription factors. The present randomized doubled-blind controlled clinical trial was performed recruiting 75 individuals with BMI 25-35, who were randomly assigned to the following three groups: Group 1 (n = 25) who consumed regular yogurt as part of a low calorie diet [RLCD], group 2 (n = 25) who received probiotic yogurt with a LCD [PLCD] and group 3 (n = 25) who consumed probiotic yogurt without LCD [PWLCD] for 8 week. Participants in PLCD and PWLCD groups received 200 g/day yogurt containing Lactobacillus acidophilus La5, Bifidobacterium Bb12, and lactobacillus casei DN001 10(8) cfu/gr. The expression of the FOXP3, T-bet, GATA3, TNF-α, IFN-γ, TGF-β, and ROR-γt in PBMCs genes were assessed, before and after intervention. In three groups, ROR-γt expression was reduced (P = 0.007) and FOXP3 was increased (P < 0.001). The expression of TNFα, TGFβ, and GATA3 genes did not change among all groups after intervention. Interestingly, the expression of T-bet gene, which was significantly decreased in PLCD and PWLCD groups (P < 0.001), whereas gene expression of IFN-γ decreased in all three groups. Our results suggest that weight loss diet and probiotic yogurt had synergistic effects on T-cell subset specific gene expression in peripheral blood mononuclear cells among overweight and obese individuals.
authors.affiliation: Department of Nutrition, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mitra
authors.initials: M
authors.lastname: Zarrati
authors.forename: Farzad
authors.initials: F
authors.lastname: Shidfar
authors.forename: Keramat
authors.initials: K
authors.lastname: Nourijelyani
authors.forename: Vahid
authors.initials: V
authors.lastname: Mofid
authors.forename: Mohammad Javad
authors.initials: MJ
authors.lastname: Hossein zadeh-Attar
authors.forename: Katayoon
authors.initials: K
authors.lastname: Bidad
authors.forename: Forouzan
authors.initials: F
authors.lastname: Najafi
authors.forename: Zahra
authors.initials: Z
authors.lastname: Gheflati
authors.forename: Maryam
authors.initials: M
authors.lastname: Chamari
authors.forename: Eisa
authors.initials: E
authors.lastname: Salehi
mesh_terms: D000328:Adult; D001644:Bifidobacterium / Q000502:physiology*; D031204:Caloric Restriction; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D004311:Double-Blind Method; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007779:Lactobacillus acidophilus / Q000502:physiology*; D007780:Lacticaseibacillus casei / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000178:diet therapy* / Q000276:immunology / Q000378:metabolism; D050177:Overweight / Q000178:diet therapy / Q000276:immunology / Q000378:metabolism; D019936:Probiotics / Q000008:administration & dosage; D013601:T-Lymphocytes / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D016896:Treatment Outcome; D015014:Yogurt; D055815:Young Adult
chemical_list: D016207:Cytokines; D014157:Transcription Factors
keywords: T lymphocyte; gene expression; low calorie diet; obesity; probiotic