title: Penetration of fosfomycin into IPEC-J2 cells in the presence or absence of deoxynivalenol.
abstract: Fosfomycin (FOS) is an antibiotic used in pig farms for treatment and prevention of infections caused by resistant bacteria during the post-weaning period. Antibiotics and non-toxic concentrations of mycotoxins, such as deoxynivalenol (DON) are frequently found in the diet of animals. These compounds can establish interactions in the intestinal tract, which could affect and/or modify the penetration of FOS to enterocytes. The aim of this study was to determine the penetration of FOS into IPECJ-2 cells, a cell line derived from the small intestine of piglets, in the presence and absence of DON. The results from this study showed that there was statistically significant difference in the intracellular concentration of FOS between cells incubated with 580 µg/ml FOS and cells incubated with 580 µg/ml FOS and 1 µg/ml DON. The Cmax of the intracellular antibiotic in the culture plates incubated with FOS in absence of DON was 45.81 µg/ml with a tmax of 4 h. When IPEC-2 cells were incubated with FOS and DON the Cmax was 20.06 µg/ml and the tmax was 30 min. It is concluded that the non-toxic concentration of DON on IPEC-J2 cells after short-term exposure, interferes with the pharmacokinetics of the antibiotic.
authors.affiliation: Centro de Investigación Veterinaria de Tandil CIVETAN-CONICET-FCV, Tandil, Buenos Aires, Argentina ; Laboratorio de Toxicología, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, Buenos Aires, Argentina.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Martínez
authors.forename: Denisa S
authors.initials: DS
authors.lastname: Pérez
authors.forename: Alejandro L
authors.initials: AL
authors.lastname: Soraci
authors.forename: María O
authors.initials: MO
authors.lastname: Tapia
mesh_terms: D000821:Animal Feed; D000818:Animals; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics*; D002460:Cell Line; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects*; D005506:Food Contamination; D005578:Fosfomycin / Q000493:pharmacokinetics*; D013552:Swine; D014255:Trichothecenes / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D014255:Trichothecenes; D005578:Fosfomycin; C007262:deoxynivalenol
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