title: Frequencies of Cytochrome P450 2B6 and 2C8 Allelic Variants in the Mozambican Population.
abstract: BACKGROUND
  The cytochrome P450 enzymes (CYP) play an important role in the metabolism of many therapeutic agents. The activities of different enzymes exhibit variability in different populations, which causes variations in drug response or toxicity. The CYP2B6 and CYP2C8 enzymes are encoded by polymorphic genes characterised by different single nucleotide polymorphisms (SNPs). Several of these CYP variants are often associated with slow metabolism phenotypes. This study aimed to analyse the frequencies of allelic variants of CYP2B6 and CYP2C8 in the Mozambican population.
  
  
  METHODS
  Using a polymerase chain reaction and restriction fragment length polymorphism assay (PCR-RFLP), the frequencies of the allelic variants of CYP2B6 (c.64C>T, c.516G>T, c.777C>A, c.785A>G, c.1459C>T) and CYP2C8 (c.805A>T, c.416G>A, c.1196A>G, c.792C>G) were determined in 360 Mozambican blood donors.
  
  
  RESULTS
  The frequencies of the allelic variants of the CYP2B6 gene were 0.057, 0.426, 0.0, 0.410, and 0.004. For the CYP2C8 gene, the frequencies of the allelic variants were 0.160, 0.048, 0.0, and 0.005. No significant differences were observed between the gender and geographic distribution of volunteers around the country.
  
  
  CONCLUSIONS
  The frequencies of the allelic variants of the CYP2B6 and CYP2C8 genes were found to be homogeneously distributed in the Mozambican population and were comparable to other African populations. Further studies are required to explore the impact of these variants on the clinical response (efficacy and toxicity) of drugs, including antimalarials.
authors.affiliation: Platform of Parasitology, Ministry of Health, National Institute of Health, P.O.Box 264 - Maputo, Mozambique.
authors.forename: Paulo
authors.initials: P
authors.lastname: Arnaldo
authors.forename: Ricardo Estevão
authors.initials: RE
authors.lastname: Thompson
authors.forename: Márcia Quinhones
authors.initials: MQ
authors.lastname: Lopes
authors.forename: Philip Noel
authors.initials: PN
authors.lastname: Suffys
authors.forename: Adalberto Rezende
authors.initials: AR
authors.lastname: Santos
keywords: CYP2B6; CYP2C8; allele frequency; polymorphism
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