title: Solid state production of polygalacturonase and xylanase by Trichoderma species using cantaloupe and watermelon rinds.
abstract: Different solid state fermentation (SSF) sources were tested such as cantaloupe and watermelon rinds, orange and banana peels, for the production of polygalacturonase (PG) and xylanase (Xyl) by Trichoderma harzianum and Trichoderma virens. The maximum production of both PG and Xyl were obtained by T. harzianum and T. virnes grown on cantaloupe and watermelon rinds, respectively. Time course, moisture content, temperature, pH, supplementation with carbon and nitrogen sources were optimized to achieve the maximum production of both PG and Xyl of T. harzianum and T. virens using cantaloupe and watermelon rinds, respectively. The maximum production of PG and Xyl of T. harzianum and T. virens was recorded at 4-5 days of incubation, 50-66% moisture, temperature 28-35°C and pH 6-7. The influence of supplementary carbon and nitrogen sources was studied. For T. harzianum, lactose enhanced PG activity from 87 to 120 units/g solid, where starch and maltose enhanced Xyl activity from 40 to 55-60 units/g solid for T. virnes. Among the nitrogen sources, ammonium sulphate, ammonium nitrate, yeast extract and urea increased PG activity from 90 to 110-113 units/g solid for T. harzianum. Similarly, ammonium chloride, ammonium sulphate and yeast extract increased Xyl activity from 45 to 55-70 units/g solid for T. virens.
authors.affiliation: Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia, saleh38@hotmail.com.
authors.forename: Saleh A
authors.initials: SA
authors.lastname: Mohamed
authors.forename: Abdulrahman L
authors.initials: AL
authors.lastname: Al-Malki
authors.forename: Jalaluddin A
authors.initials: JA
authors.lastname: Khan
authors.forename: Saleh A
authors.initials: SA
authors.lastname: Kabli
authors.forename: Saleh M
authors.initials: SM
authors.lastname: Al-Garni
mesh_terms: D002244:Carbon / Q000378:metabolism; D029773:Citrullus / Q000382:microbiology; D028463:Cucumis melo / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000378:metabolism; D011096:Polygalacturonase / Q000378:metabolism*; D013696:Temperature; D014242:Trichoderma / Q000201:enzymology* / Q000254:growth & development; D014995:Xylosidases / Q000378:metabolism*
chemical_list: D002244:Carbon; D014995:Xylosidases; D011096:Polygalacturonase; D009584:Nitrogen
references.citation: Ecotoxicol Environ Saf. 2006 Jun;64(2):244-50
references.pmid: 16406599
references.citation: Appl Biochem Biotechnol. 2008 Mar;146(1-3):119-28
references.pmid: 18421592
references.citation: Bioresour Technol. 2011 Nov;102(21):10085-8
references.pmid: 21908185
references.citation: Bioresour Technol. 2008 May;99(7):2373-83
references.pmid: 17604624
references.citation: Bioresour Technol. 2006 Dec;97(18):2340-4
references.pmid: 16337373
references.citation: Bioresour Technol. 2005 Aug;96(12):1380-6
references.pmid: 15792586
references.citation: Bioresour Technol. 2011 Nov;102(22):10657-62
references.pmid: 21945204
references.citation: Bioresour Technol. 2001 Jul;78(3):277-80
references.pmid: 11341688
references.citation: J Biosci Bioeng. 2002;93(1):9-14
references.pmid: 16233157
references.citation: J Biotechnol. 2007 Jan 1;127(2):322-34
references.pmid: 16945442