title: Genomic investigation into strain heterogeneity and pathogenic potential of the emerging gastrointestinal pathogen Campylobacter ureolyticus.
abstract: The recent detection and isolation of C. ureolyticus from patients with diarrhoeal illness and inflammatory bowel diseases warrants further investigation into its role as an emerging pathogen of the human gastrointestinal tract. Regarding the pathogenic mechanisms employed by this species we provide the first whole genome analysis of two C. ureolyticus isolates including the type strain. Comparative analysis, subtractive hybridisation and gene ontology searches against other Campylobacter species identifies the high degree of heterogenicity between C. ureolyticus isolates, in addition to the identification of 106 putative virulence associated factors, 52 of which are predicted to be secreted. Such factors encompass each of the known virulence tactics of pathogenic Campylobacter spp. including adhesion and colonisation (CadF, PEB1, IcmF and FlpA), invasion (ciaB and 16 virB-virD4 genes) and toxin production (S-layer RTX and ZOT). Herein, we provide the first virulence catalogue for C. ureolyticus, the components of which theoretically provide this emerging species with sufficient arsenal to establish pathology.
authors.affiliation: Department of Biological Sciences, Cork Institute of Technology, Cork, Ireland.
authors.forename: Susan
authors.initials: S
authors.lastname: Bullman
authors.forename: Alan
authors.initials: A
authors.lastname: Lucid
authors.forename: Daniel
authors.initials: D
authors.lastname: Corcoran
authors.forename: Roy D
authors.initials: RD
authors.lastname: Sleator
authors.forename: Brigid
authors.initials: B
authors.lastname: Lucey
mesh_terms: D000992:Antitoxins / Q000235:genetics; D001422:Bacterial Adhesion / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D058947:Bacterial Secretion Systems / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D002167:Campylobacter / Q000235:genetics* / Q000472:pathogenicity*; D002169:Campylobacter Infections / Q000235:genetics / Q000382:microbiology*; D019295:Computational Biology; D017124:Conserved Sequence / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D019143:Evolution, Molecular; D041981:Gastrointestinal Tract / Q000382:microbiology* / Q000473:pathology*; D005798:Genes, Bacterial / Q000235:genetics; D014644:Genetic Variation; D016680:Genome, Bacterial / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D012995:Solubility; D026801:Synteny / Q000235:genetics; D014774:Virulence / Q000235:genetics
chemical_list: D000992:Antitoxins; D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems; D001427:Bacterial Toxins
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