title: CCR7 expression and intratumoral FOXP3+ regulatory T cells are correlated with overall survival and lymph node metastasis in gastric cancer.
abstract: The aim of this study was to investigate the prognostic value of chemokine receptor CCR7 expression and intratumoral FOXP3(+) regulatory T cells (Tregs) in gastric cancer. CCR7(+) tumor cells and FOXP3(+) Tregs were assessed by immunohistochemistry in tissue microarrays containing gastric cancer from 133 patients. Prognostic effects of low or high CCR7 and FOXP3 expression were evaluated by Cox regression and Kaplan-Meier analysis, as well as the correlation between CCR7 positive score and intratumoral FOXP3(+) cell number in a longitudinal assessment. The analysis showed that the high expression levels of CCR7 and FOXP3 were detected in 69.9% and 65.4% of cases, respectively. High CCR7 expression in gastric cancer cells was significantly associated with poor overall survival (OS) (P = 0.010) and lymph node metastasis (P = 0.009), and was an independent factor for worse OS (P = 0.023) by multivariate analysis. High numbers of intratumoral FOXP3(+) Tregs significantly correlated with shorter OS (P = 0.021) and lymph node metastasis (P = 0.024), and was also an independent factor for adverse OS (P = 0.035). Furthermore, there was a significantly positive correlation between CCR7 positive score and intratumoral FOXP3(+) cell number (r = 0.949, P<0.001). These results revealed that CCR7 expression in gastric cancer cells and intratumoral FOXP3(+) Tregs could be considered as a co-indicator of clinical prognosis of gastric cancer.
authors.affiliation: Department of Histology and Embryology, Tongji University School of Medicine, Shanghai, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhou
authors.forename: Zhenbing
authors.initials: Z
authors.lastname: Shen
authors.forename: Yanna
authors.initials: Y
authors.lastname: Wang
authors.forename: Huiying
authors.initials: H
authors.lastname: Ma
authors.forename: Shuchang
authors.initials: S
authors.lastname: Xu
authors.forename: Jie
authors.initials: J
authors.lastname: Qin
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.forename: Huihong
authors.initials: H
authors.lastname: Tao
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Zhen
authors.forename: Guolin
authors.initials: G
authors.lastname: Chen
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Rilun
authors.initials: R
authors.lastname: Li
authors.forename: Honglei
authors.initials: H
authors.lastname: Xiao
authors.forename: Cuiping
authors.initials: C
authors.lastname: Zhong
authors.forename: Yaoqin
authors.initials: Y
authors.lastname: Yang
authors.forename: Chunmin
authors.initials: C
authors.lastname: Liang
mesh_terms: D000368:Aged; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D016246:Lymphocytes, Tumor-Infiltrating / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D054400:Receptors, CCR7 / Q000235:genetics*; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism* / Q000473:pathology; D046888:Tissue Array Analysis
chemical_list: C515370:CCR7 protein, human; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D054400:Receptors, CCR7
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