title: The effect of different growth regimes on the endophytic bacterial communities of the fern, Dicksonia sellowiana hook (Dicksoniaceae).
abstract: Endophytic bacteria associated with the fern Dicksonia sellowiana were investigated. The bacterial communities from the surface-sterilized pinnae and rachis segments of the plants from the Brazilian Atlantic Rainforest that grew in native field conditions were compared with the bacterial communities from plants grown in greenhouses and plants that were initially grown in greenhouses and then transferred to the forest. From 540 pinnae and 540 rachis segments, 163 (30.2%) and 346 (64.2%) were colonized by bacteria, respectively. The main bacterial genera and species that were isolated included Bacillus spp. ( B. cereus, B. megaterium, B. pumilus and B. subtilis ) , Paenibacillus sp. , Amphibacillus sp. , Gracilibacillus sp. , Micrococcus sp. and Stenotrophomonas spp. ( S. maltophilia and S. nitroreducens ). B. pumilus was the most frequently isolated bacterial species . Amphibacillus and Gracilibacillus were reported as endophytes for the first time. Other commonly found bacterial genera were not observed in D. sellowiana , which may reflect preferences of specific bacterial communities inside this fern or detection limitations due to the isolation procedures. Plants that were grown in greenhouses and plants that were reintroduced into the forest displayed more bacterial genera and species diversity than native field plants, suggesting that reintroduction shifts the bacterial diversity. Endophytic bacteria that displayed antagonistic properties against different microorganisms were detected, but no obvious correlation was found between their frequencies with plant tissues or with plants from different growth regimes. This paper reports the first isolation of endophytic bacteria from a fern.
authors.affiliation: Laboratório de Biologia Molecular e Ecologia Microbiana, Núcleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes , Mogi das Cruzes, SP , Brasil.
authors.forename: Irene
authors.initials: I
authors.lastname: de Araújo Barros
authors.forename: Welington
authors.initials: W
authors.lastname: Luiz Araújo
authors.forename: João
authors.initials: J
authors.lastname: Lúcio Azevedo
keywords: 16S rDNA; Bacillus; Brazilian Tropical forest; bacterial diversity; ferns
references.citation: J Nat Prod. 2004 Feb;67(2):257-68
references.pmid: 14987067
references.citation: Can J Microbiol. 2001 Mar;47(3):229-36
references.pmid: 11315114
references.citation: Crit Rev Biotechnol. 2002;22(4):315-33
references.pmid: 12487423
references.citation: Annu Rev Microbiol. 2002;56:117-37
references.pmid: 12142491
references.citation: New Phytol. 1992 Oct;122(2):299-305
references.pmid: 33873992
references.citation: Appl Environ Microbiol. 2002 May;68(5):2198-208
references.pmid: 11976089
references.citation: Can J Microbiol. 2006 May;52(5):419-26
references.pmid: 16699566
references.citation: FEMS Microbiol Lett. 2008 Jan;278(1):1-9
references.pmid: 18034833
references.citation: FEMS Microbiol Lett. 2008 Oct;287(1):8-14
references.pmid: 18710397
references.citation: Appl Environ Microbiol. 2002 Oct;68(10):4906-14
references.pmid: 12324338
references.citation: Environ Microbiol. 2004 Dec;6(12):1244-51
references.pmid: 15560822
references.citation: Mol Biol Evol. 1987 Jul;4(4):406-25
references.pmid: 3447015
references.citation: Braz J Microbiol. 2009 Jul;40(3):417-32
references.pmid: 24031382
references.citation: New Phytol. 1996 Jan;132(1):119-122
references.pmid: 33863046
references.citation: Microbiol Mol Biol Rev. 2003 Dec;67(4):491-502
references.pmid: 14665674
references.citation: Microbiol Rev. 1995 Jun;59(2):171-200
references.pmid: 7603408