title: Mechanical compressive force inhibits adipogenesis of adipose stem cells.
abstract: OBJECTIVE
  The aim of this study was to investigate effects of mechanical compressive force on differentiation of adipose-derived stem cells (ASCs) in vitro.
  
  
  METHODS
  Mice ASCs were treated with compressive force (2000 με, 1 Hz) for 2 or 6 h after adipogenic induction for 3 days, then oil red O staining was used to examine oil droplet-filled cells. Adipogenic genes, PPAR-γ1 and APN, were examined by real-time PCR and immunofluorescence (IF) staining was performed to test expression of de-PPAR-γ and ph-PPAR-γ at the protein level.
  
  
  RESULTS
  Our data showed that mechanical compressive force reduced numbers of oil droplet-filled cells, and down-regulated mRNA levels of both PPAR-γ1 and APN and protein level of PPAR-γ, in ASCs.
  
  
  CONCLUSIONS
  In culture medium containing adipogenic stimuli, mechanical compressive force inhibited adipogenesis of ASCs.
authors.affiliation: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
authors.forename: G
authors.initials: G
authors.lastname: Li
authors.forename: N
authors.initials: N
authors.lastname: Fu
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: K
authors.initials: K
authors.lastname: Ba
authors.forename: X
authors.initials: X
authors.lastname: Wei
authors.forename: Y
authors.initials: Y
authors.lastname: Fu
authors.forename: Y
authors.initials: Y
authors.lastname: Yao
authors.forename: X
authors.initials: X
authors.lastname: Cai
authors.forename: Y
authors.initials: Y
authors.lastname: Lin
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis; D052242:Adiponectin / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D051379:Mice; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D013314:Stress, Mechanical
chemical_list: D052242:Adiponectin; D047495:PPAR gamma
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