title: Comparison of agglutinating and neutralizing antibodies to serovar hardjo in sows immunized with two commercial whole culture polivalent anti-leptospira bacterins.
abstract: It was performed the comparison of the intensity and duration of agglutinating and neutralizing antibodies to serovar Hardjo in swines vaccinated with two commercial anti-leptospira bacterins. Sows no reactive to 24 Leptospira sp serovars in the microscopic agglutination test (MAT) were divided in three groups: Group A (n=08): received two vaccine A doses with 30 days interval, Group B (n=08) two vaccine B doses with 30 days interval and Group C (n=08): control no vaccinated against leptospirosis.Blood samples were collected each 30 days during six months following the first vaccination. The sera were tested by MAT and growth inhibition test (GIT) to serovar Hardjo in order to evaluate respectively agglutinating and neutralizing antibodies. It was found that neutralizing antibodies persisted for a longer time than the agglutinating ones and that the absence of agglutinating antibodies does not means in the absence of the neutralizing. The peaks of agglutinating antibodies was obtained at least 30 days earlier than that produced by neutralizing. The duration of both kinds of antibodies measured differed between the two bacterines tested. The period for inducing neutralizing antibodies against serovar Hardjo indicated that gilts must be immunized with two doses of whole culture anti-leptospira bacterines applied 30 days each other at least 90 days before the first mating. For the maintenance of hight levels of neutralizing antibodies the revaccinations must be performed every six months after the first vaccination.
authors.affiliation: Centro de Vigilância Sanitária e Controle de Zoonoses "Tereza Rodrigues de Camargo" , Ibiúna, SP , Brasil.
authors.forename: Francisco Rafael Martins
authors.initials: FR
authors.lastname: Soto
authors.forename: Sônia Regina
authors.initials: SR
authors.lastname: Pinheiro
authors.forename: Zenaide Maria
authors.initials: ZM
authors.lastname: Morais
authors.forename: Amane Paldês
authors.initials: AP
authors.lastname: Gonçales
authors.forename: Sérgio Santos
authors.initials: SS
authors.lastname: de Azevedo
authors.forename: Fernanda
authors.initials: F
authors.lastname: Bernardi
authors.forename: Sebastião Rodrigues
authors.initials: SR
authors.lastname: Camargo
authors.forename: Silvio Arruda
authors.initials: SA
authors.lastname: Vasconcellos
keywords: bacterines; immunity; leptospirosis; swine; vaccination
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