title: Growth, fatty acid profile in major lipid classes and lipid fluidity of Aurantiochytrium mangrovei SK-02 As a function of growth temperature.
abstract: Aurantiochytrium mangrovei Sk-02 was grown in a medium containing glucose (40 g/l), yeast extract (10 g/L) and sea salts (15 g/L) at temperatures ranging from 12 to 35°C. The fastest growth (µmax= 0.15 h(-1)) and highest fatty acid content of 415 mg/g-dry cell weight were found in the cells grown at 30°C. However, the cells grown at 12°C showed the highest percentage of polyunsaturated fatty acid (PUFA) (48.6% of total fatty acid). The percentage of docosahexaenoic acid (DHA) and pentadecanoic acid (C15:0) decreased with an increase in the growth temperature, whereas, palmitic acid (C16:0), stearic acid (C18:0) and DPA (C22:5n6) increased with an increase in the growth temperature. The composition of the major lipid class (%w/w) was slightly affected by the growth temperature. The fluidity of the organelle membrane or intracellular lipid (by DPH measurement) decreased with an increase in the growth temperatures, while the plasma membrane fluidity (by TMA-DPH measurement) could still maintain its fluidity in a wide range of temperatures (15 - 37°C). Furthermore, the distribution of DHA was found to be higher (36 - 54%) in phospholipid (PL) as compared to neutral lipid (NL) (20 - 41%).
authors.affiliation: Department of Biotechnology, Faculty of Science, Mahidol University , Bangkok , Thailand.
authors.forename: Kanokwan
authors.initials: K
authors.lastname: Chodchoey
authors.forename: Cornelis
authors.initials: C
authors.lastname: Verduyn
keywords: Anisotropy measurement; Aurantiochytrium sp.>; Docosahexaenoic acid; lipid class composition; membrane fluidity
references.citation: Science. 2001 Jul 13;293(5528):290-3
references.pmid: 11452122
references.citation: Can J Biochem Physiol. 1959 Aug;37(8):911-7
references.pmid: 13671378
references.citation: J Agric Food Chem. 2007 Apr 18;55(8):2906-10
references.pmid: 17381126
references.citation: Lipids. 2005 Jun;40(6):609-18
references.pmid: 16149740
references.citation: Mar Biotechnol (NY). 2010 Apr;12(2):173-85
references.pmid: 19609613
references.citation: Prostaglandins Leukot Essent Fatty Acids. 2003 Feb;68(2):181-6
references.pmid: 12538082
references.citation: Appl Microbiol Biotechnol. 2007 Oct;76(5):985-90
references.pmid: 17694304
references.citation: Appl Microbiol Biotechnol. 2004 Apr;64(2):146-53
references.pmid: 14740186
references.citation: J Lipid Res. 1985 Jan;26(1):135-40
references.pmid: 3973509
references.citation: Explore (NY). 2009 Sep-Oct;5(5):299-303
references.pmid: 19733817
references.citation: Appl Microbiol Biotechnol. 2008 Aug;80(2):297-306
references.pmid: 18560831
references.citation: Prog Lipid Res. 2000 Jul;39(4):315-91
references.pmid: 10856601
references.citation: Appl Environ Microbiol. 1999 Apr;65(4):1710-20
references.pmid: 10103272
references.citation: Altern Med Rev. 2007 Sep;12(3):207-27
references.pmid: 18072818
references.citation: Mar Biotechnol (NY). 2009 May-Jun;11(3):368-74
references.pmid: 18941836
references.citation: Biochimie. 2004 Nov;86(11):807-15
references.pmid: 15589690
references.citation: Biochim Biophys Acta. 2008 Apr;1778(4):1141-7
references.pmid: 18187036
references.citation: J Microbiol Methods. 2000 Dec 15;43(2):107-16
references.pmid: 11121609
references.citation: Biotechnol Lett. 2007 Dec;29(12):1977-81
references.pmid: 17660943
references.citation: Mol Biol Evol. 2007 Aug;24(8):1596-9
references.pmid: 17488738
references.citation: J Lipid Res. 1995 Nov;36(11):2433-43
references.pmid: 8656081