title: Markers of iron metabolism in retired racing Greyhounds with and without osteosarcoma.
abstract: BACKGROUND
  Greyhounds have well-described clinicopathologic idiosyncrasies, including a high prevalence of osteosarcoma (OSA). Hematocrit, HGB, and HGB oxygen affinity are higher than in other dogs, while haptoglobin concentration is lower, so we hypothesized that Greyhounds have a different iron metabolism. To our knowledge, there are no reports on serum iron profiles in Greyhounds.
  
  
  OBJECTIVE
  To elucidate iron metabolism in Greyhounds, we wanted to compare serum iron concentration, total iron-binding capacity (TIBC), and percent transferrin saturation (%SAT) in healthy retired racing Greyhounds (RRGs) with OSA (RRGs - OSA), and also with non-Greyhounds (NGs), without and with OSA (NGs - OSA).
  
  
  METHODS
  Serum iron concentration and unsaturated iron-binding capacity (UIBC) were measured by standard methods, and TIBC and %SAT were calculated in RRGs (n = 25), RRGs - OSA (n = 28), NGs (n = 30), and NGs - OSA (n = 32).
  
  
  RESULTS
  TIBC was lower in RRGs than in NGs (P < .0001), and in RRGs - OSA than in NGs - OSA (P < .0001). NGs - OSA had lower TIBC than healthy NGs (P = .003). Percent SAT was higher in RRGs than in NGs (P < .0001) and in RRGs - OSA (P = .008), and %SAT was also lower in NGs than in NGs - OSA (P = .004). Percent SAT was also higher in RRGs - OSA than in NGs - OSA (P = .001). Both RRGs - OSA (P = .02) and NGs - OSA (P < .0001) had lower serum iron concentrations than their healthy counterparts.
  
  
  CONCLUSIONS
  Lower TIBC and higher %SAT may constitute another Greyhound idiosyncrasy compared with other dogs. In this study, all dogs with OSA had higher serum iron concentrations and %SAT than healthy dogs.
authors.affiliation: Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, OH, USA.
authors.forename: J T
authors.initials: JT
authors.lastname: Caro
authors.forename: L M
authors.initials: LM
authors.lastname: Marín
authors.forename: M C
authors.initials: MC
authors.lastname: Iazbik
authors.forename: S
authors.initials: S
authors.lastname: Zaldivar-López
authors.forename: H
authors.initials: H
authors.lastname: Borghese
authors.forename: C G
authors.initials: CG
authors.lastname: Couto
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D004283:Dog Diseases / Q000097:blood* / Q000378:metabolism; D004285:Dogs; D007501:Iron / Q000097:blood* / Q000378:metabolism; D033862:Iron-Binding Proteins / Q000097:blood* / Q000378:metabolism; D012516:Osteosarcoma / Q000097:blood / Q000378:metabolism / Q000662:veterinary*; D014168:Transferrin / Q000378:metabolism
chemical_list: D015415:Biomarkers; D033862:Iron-Binding Proteins; D014168:Transferrin; D007501:Iron
keywords: Dihydroartemisinin; iron sequestration; percent transferrin saturation; serum iron concentration; total iron-binding capacity
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