title: In vitro replication competence of a hepatitis B genotype D/A recombinant virus: dissimilar biological behaviour regarding its parental genotypes.
abstract: Hepatitis B virus (HBV) DNA recombinants contribute to ~30% of the overall full-length sequences already deposited in GenBank. However, their biological behaviour has not been analysed so far. In this study, the in vitro replication kinetics of the first D/A recombinant from the American continent differed from its parental genotypes, exhibiting higher extracellular levels of HBV DNA and hepatitis B e antigen. Southern blots of intracellular core-associated HBV DNA were in agreement with such results. Because this recombinant was obtained from an Argentinian injecting drug user belonging to a vulnerable community, these results are of singular relevance for regional public health. Further in vivo studies are urgently needed to determine the pathogenicity of these replicative competent clones.
authors.affiliation: Instituto de Microbiología y Parasitología Médica (IMPAM), Universidad de Buenos Aires (UBA) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Paraguay 2155, Piso 11, 1121, Buenos Aires, Argentina.
authors.forename: Julieta
authors.initials: J
authors.lastname: Trinks
authors.affiliation: Research Center for Hepatitis and Immunology, National Center for Global Health and Medicine, 1-7-1, Kohnodai, Ichikawa 272-8516, Japan.|Department of Virology and Liver Unit, Nagoya City University Graduate School of Medical Sciences, Kawasumi, Mizuho, Nagoya 467-8601, Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Sugiyama
authors.affiliation: Department of Virology and Liver Unit, Nagoya City University Graduate School of Medical Sciences, Kawasumi, Mizuho, Nagoya 467-8601, Japan.
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Tanaka
authors.affiliation: Department of Virology and Liver Unit, Nagoya City University Graduate School of Medical Sciences, Kawasumi, Mizuho, Nagoya 467-8601, Japan.
authors.forename: Fuat
authors.initials: F
authors.lastname: Kurbanov
authors.affiliation: Fundación de Ayuda al Inmunodeficiente (FUNDAI), Uspallata 2272, 1282, Buenos Aires, Argentina.
authors.forename: Jorge
authors.initials: J
authors.lastname: Benetucci
authors.affiliation: Hospital Zonal General de Agudos 'Dr Isidoro G. Iriarte', Allison Bell 770, 1878, Quilmes, Argentina.
authors.forename: Edgardo
authors.initials: E
authors.lastname: Giménez
authors.affiliation: National Academy of Medicine, Av. Las Heras 3092, 1425, Buenos Aires, Argentina.|Instituto de Microbiología y Parasitología Médica (IMPAM), Universidad de Buenos Aires (UBA) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Paraguay 2155, Piso 11, 1121, Buenos Aires, Argentina.
authors.forename: Mercedes C
authors.initials: MC
authors.lastname: Weissenbacher
authors.affiliation: Research Center for Hepatitis and Immunology, National Center for Global Health and Medicine, 1-7-1, Kohnodai, Ichikawa 272-8516, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Mizokami
authors.affiliation: Instituto de Microbiología y Parasitología Médica (IMPAM), Universidad de Buenos Aires (UBA) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Paraguay 2155, Piso 11, 1121, Buenos Aires, Argentina.
authors.forename: José R
authors.initials: JR
authors.lastname: Oubiña
mesh_terms: D001118:Argentina; D001483:Base Sequence; D004279:DNA, Viral / Q000097:blood / Q000302:isolation & purification; D005838:Genotype; D006513:Hepatitis B e Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics / Q000502:physiology*; D019694:Hepatitis B, Chronic / Q000821:virology; D006801:Humans; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D006513:Hepatitis B e Antigens