title: Repopulation of intrasynovial flexor tendon allograft with bone marrow stromal cells: an ex vivo model.
abstract: OBJECTIVE
  Delayed healing is a common problem whenever tendon allografts are used for tendon or ligament reconstruction. Repopulating the allograft with host cells may accelerate tendon regeneration, but cell penetration into the allograft tendon is limited. Processing the tendon surface with slits that guide cells into the allograft substrate may improve healing. The purpose of this study was to describe a surface modification of allograft tendon that includes slits to aid cell repopulation and lubrication to enhance tendon gliding.
  
  
  METHODS
  Canine flexor digitorum profundus tendons were used for this study. Cyclic gliding resistance was measured over 1000 cycles. Tensile stiffness was assessed for normal tendon, tendon decellularized with trypsin and Triton X-100 (decellularized group), tendon decellularized and perforated with multiple slits (MS group) and tendon decellularized, perforated with slits and treated with a carbodiimide-derivatized hyaluronic acid and gelatin (cd-HA-gelatin) surface modification (MS-SM group). To assess tendon repopulation, bone marrow stromal cells (BMSCs) were used in the decellularized and MS groups. DNA concentration and histology were evaluated and compared to normal tendons and nonseeded decellularized tendons.
  
  
  RESULTS
  The gliding resistance of the decellularized and MS groups was significantly higher compared with the normal group. There was no significant difference in gliding resistance between the decellularized and MS group. Gliding resistance of the normal group and MS-SM group was not significantly different. The Young's modulus was not significantly different among the four groups. The DNA concentration in the MS group was significantly lower than in normal tendons, but significantly higher than in decellularized tendons, with or without BMSCs. Viable BMSCs were found in the slits after 2 weeks in tissue culture.
  
  
  CONCLUSIONS
  Tendon slits can successfully harbor BMSCs without compromising their survival and without changing tendon stiffness. Surface modification restores normal gliding function to the slit tendon.
  
  
  CONCLUSIONS
  A multislit tendon reseeded with BMSCs, with a surface treatment applied to restore gliding properties, may potentially promote tendon revitalization and accelerate healing for tendon or ligament reconstruction applications.
authors.affiliation: Biomechanics & Tendon and Soft Tissue Laboratories, Department of Orthopedic Surgery, Mayo Clinic Rochester , Rochester, Minnesota.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ozasa
authors.forename: Peter C
authors.initials: PC
authors.lastname: Amadio
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Thoreson
authors.forename: Kai-Nan
authors.initials: KN
authors.lastname: An
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Zhao
mesh_terms: D064591:Allografts / Q000502:physiology*; D000818:Animals; D002470:Cell Survival; D004247:DNA / Q000378:metabolism; D004285:Dogs; D055119:Elastic Modulus; D017276:Friction; D006801:Humans; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D023421:Models, Animal; D013583:Synovial Membrane / Q000502:physiology*; D013710:Tendons / Q000502:physiology*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D004247:DNA
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