title: Matrix production and organization by endothelial colony forming cells in mechanically strained engineered tissue constructs.
abstract: OBJECTIVE
  Tissue engineering is an innovative method to restore cardiovascular tissue function by implanting either an in vitro cultured tissue or a degradable, mechanically functional scaffold that gradually transforms into a living neo-tissue by recruiting tissue forming cells at the site of implantation. Circulating endothelial colony forming cells (ECFCs) are capable of differentiating into endothelial cells as well as a mesenchymal ECM-producing phenotype, undergoing Endothelial-to-Mesenchymal-transition (EndoMT). We investigated the potential of ECFCs to produce and organize ECM under the influence of static and cyclic mechanical strain, as well as stimulation with transforming growth factor β1 (TGFβ1).
  
  
  RESULTS
  A fibrin-based 3D tissue model was used to simulate neo-tissue formation. Extracellular matrix organization was monitored using confocal laser-scanning microscopy. ECFCs produced collagen and also elastin, but did not form an organized matrix, except when cultured with TGFβ1 under static strain. Here, collagen was aligned more parallel to the strain direction, similar to Human Vena Saphena Cell-seeded controls. Priming ECFC with TGFβ1 before exposing them to strain led to more homogenous matrix production.
  
  
  CONCLUSIONS
  Biochemical and mechanical cues can induce extracellular matrix formation by ECFCs in tissue models that mimic early tissue formation. Our findings suggest that priming with bioactives may be required to optimize neo-tissue development with ECFCs and has important consequences for the timing of stimuli applied to scaffold designs for both in vitro and in situ cardiovascular tissue engineering. The results obtained with ECFCs differ from those obtained with other cell sources, such as vena saphena-derived myofibroblasts, underlining the need for experimental models like ours to test novel cell sources for cardiovascular tissue engineering.
authors.affiliation: Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.
authors.forename: Nicky
authors.initials: N
authors.lastname: de Jonge
authors.forename: Dimitri E P
authors.initials: DE
authors.lastname: Muylaert
authors.forename: Emanuela S
authors.initials: ES
authors.lastname: Fioretta
authors.forename: Frank P T
authors.initials: FP
authors.lastname: Baaijens
authors.forename: Joost O
authors.initials: JO
authors.lastname: Fledderus
authors.forename: Marianne C
authors.initials: MC
authors.lastname: Verhaar
authors.forename: Carlijn V C
authors.initials: CV
authors.lastname: Bouten
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D004549:Elastin / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D016228:Endothelial Growth Factors / Q000494:pharmacology; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D057054:Molecular Imaging; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D013314:Stress, Mechanical; D023822:Tissue Engineering; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
chemical_list: D015415:Biomarkers; D016228:Endothelial Growth Factors; D053773:Transforming Growth Factor beta1; D003094:Collagen; D004549:Elastin
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