title: Glass bead transformation method for gram-positive bacteria.
abstract: A simple, inexpensive and reproducible transformation method was developed for Gram-positive bacteria. It was based on agitation of bacterial protoplasts with glass beads in the presence of DNA and polyethylene glycol. By using this method, introduction of pGK12 into protoplasts of several strains of Gram-positive bacteria was achieved.
authors.affiliation: Department of Biological Science, Ubon Ratchathani University , Warin Chamrap, Ubon Ratchathani 34190 , Thailand.
authors.forename: Pongsak
authors.initials: P
authors.lastname: Rattanachaikunsopon
authors.forename: Parichat
authors.initials: P
authors.lastname: Phumkhachorn
keywords: Gram-positive bacteria; glass bead; transformation
references.citation: J Microbiol Methods. 2005 Mar;60(3):353-63
references.pmid: 15649537
references.citation: FEMS Microbiol Lett. 1991 Apr 15;63(2-3):323-7
references.pmid: 1905659
references.citation: J Appl Microbiol. 2007 Mar;102(3):736-47
references.pmid: 17309623
references.citation: FEMS Microbiol Lett. 2004 Dec 1;241(1):73-7
references.pmid: 15556712
references.citation: Appl Environ Microbiol. 1984 Aug;48(2):252-9
references.pmid: 6091544
references.citation: Methods Mol Biol. 1995;47:201-8
references.pmid: 7550736
references.citation: Appl Environ Microbiol. 1989 Dec;55(12):3119-23
references.pmid: 16348073
references.citation: Mol Microbiol. 1988 Sep;2(5):637-46
references.pmid: 3185282
references.citation: Biotechnol Lett. 2004 Jul;26(14):1119-24
references.pmid: 15266116
references.citation: Lett Appl Microbiol. 1996 Feb;22(2):159-61
references.pmid: 8936377
references.citation: Appl Environ Microbiol. 1991 Apr;57(4):1194-201
references.pmid: 1905518
references.citation: J Microbiol Methods. 2006 Dec;67(3):543-8
references.pmid: 16820234
references.citation: Appl Environ Microbiol. 1986 Aug;52(2):394-5
references.pmid: 3092737
references.citation: Annu Rev Microbiol. 1981;35:237-72
references.pmid: 7027901
references.citation: J Bacteriol. 1986 Mar;165(3):831-6
references.pmid: 3005240
references.citation: J Microbiol Methods. 2006 Sep;66(3):556-9
references.pmid: 16503058
references.citation: Plasmid. 1987 Jan;17(1):73-5
references.pmid: 3575503