title: Perturbation of biogenesis and targeting of Epstein-Barr virus-encoded miR-BART3 microRNA by adenosine-to-inosine editing.
abstract: Epstein-Barr virus (EBV) encodes at least 44 mature microRNAs (miRNAs), some of which are abundantly expressed in nasopharyngeal carcinoma cells. EBV-encoded miR-BART6 miRNA is known to undergo adenosine-to-inosine (A-to-I) RNA editing, which impacts on processing and function. Whether additional EBV miRNAs might be A-to-I edited remains to be determined. In this study, we have reported on A-to-I editing of EBV miR-BART3. The A-to-I editing enzyme was expressed abundantly in EBV-infected epithelial carcinoma cells. pri-miR-BART3 was found to be edited at four sites in these cells and in nasopharyngeal carcinoma samples. Whereas editing of the second site located within the seed region prevented the targeting of DICE1 mRNA, editing of the third site effectively crippled the biogenesis of mature miR-BART3. Thus, A-to-I editing perturbs biogenesis and targeting of miR-BART3 and may contribute to its differential expression and function in EBV-infected epithelial cells.
authors.affiliation: Department of Biochemistry, University of Hong Kong, Pokfulam, Hong Kong SAR, PR China.|Department of Pathology, School of Medicine, Xi'an Jiaotong University, Xi'an, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Lei
authors.affiliation: Department of Biochemistry, University of Hong Kong, Pokfulam, Hong Kong SAR, PR China.
authors.forename: Kit-San
authors.initials: KS
authors.lastname: Yuen
authors.affiliation: Department of Anatomy, University of Hong Kong, Pokfulam, Hong Kong SAR, PR China.
authors.forename: Sai Wah
authors.initials: SW
authors.lastname: Tsao
authors.affiliation: Department of Microbiology, University of Hong Kong, Pokfulam, Hong Kong SAR, PR China.
authors.forename: Honglin
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Biochemistry, University of Hong Kong, Pokfulam, Hong Kong SAR, PR China.
authors.forename: Kin-Hang
authors.initials: KH
authors.lastname: Kok
authors.affiliation: Department of Biochemistry, University of Hong Kong, Pokfulam, Hong Kong SAR, PR China.
authors.forename: Dong-Yan
authors.initials: DY
authors.lastname: Jin
mesh_terms: D000241:Adenosine / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002277:Carcinoma; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D020031:Epstein-Barr Virus Infections / Q000821:virology; D057809:HEK293 Cells; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D007288:Inosine / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms; D017393:RNA Editing; D014157:Transcription Factors
chemical_list: C485676:ARL2BP protein, human; D002352:Carrier Proteins; D035683:MicroRNAs; D014157:Transcription Factors; D007288:Inosine; D000241:Adenosine