title: Defining the seriousness of proliferative vitreoretinopathy by aspiration of cytokines from the anterior chamber.
abstract: OBJECTIVE
  Proliferative vitreoretinopathy is the major cause of retinal detachment surgery failure. Our prospective cohort study of 27 eyes aimed to determine intraocular levels of growth factors and cytokines in patients with retinal detachment with various degrees of severity of proliferative vitreoretinopathy using multiplex xMAP(®) Technology.
  
  
  METHODS
  The concentrations of 12 proangiogenic, antiangiogenic, inflammatory and neurotrophic factors were measured from 0.05-ml samples of intraocular fluid using multiplex xMAP Technology. The results were compared with levels of various factors, which were measured in samples from the control group of 31 eyes prior to senile cataract surgery.
  
  
  RESULTS
  The concentration of the MCP-1 cytokine was found to be higher in eyes with retinal detachment compared with the control group. The concentration of VEGF was found to be higher in eyes with retinal detachment complicated with proliferative vitreoretinopathy compared with the uncomplicated retinal detachment group and the control group.
  
  
  CONCLUSIONS
  MCP-1 and VEGF may participate in pathogenesis of retinal detachment and proliferative vitreoretinopathy. Biomarkers in disease detection and management have become important tools in modern clinical medicine, and their application to retinal disease should be no exception.
authors.affiliation: Department of Ophthalmology, University Hospital in Pilsen, Alej Svobody 80, Pilsen, 304 60, Czech Republic.
authors.forename: Stepan
authors.initials: S
authors.lastname: Rusnak
authors.forename: Jindra
authors.initials: J
authors.lastname: Vrzalova
authors.forename: Lenka
authors.initials: L
authors.lastname: Hecová
authors.forename: Marketa
authors.initials: M
authors.lastname: Kozova
authors.forename: Ondrej
authors.initials: O
authors.lastname: Topolcan
authors.forename: Renata
authors.initials: R
authors.lastname: Ricarova
mesh_terms: D000368:Aged; D000867:Anterior Chamber / Q000378:metabolism* / Q000473:pathology*; D001707:Biopsy, Needle; D018932:Chemokine CCL2 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012163:Retinal Detachment / Q000150:complications; D018630:Vitreoretinopathy, Proliferative / Q000150:complications / Q000378:metabolism* / Q000473:pathology*
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D016207:Cytokines