title: Transcriptome profiling reveals Th17-like immune responses induced in zebrafish bath-vaccinated with a live attenuated Vibrio anguillarum.
abstract: BACKGROUND
  A candidate vaccine, live attenuated Vibrio anguillarum developed in our laboratory could prevent vibriosis of fish resulted from V. anguillarum and V. alginolyticus. To elucidate the molecular mechanisms underlying the vaccine protection, we used microarray technology to compare the spleen transcriptomes of bath-vaccinated and unvaccinated zebrafish at 28 days post vaccination.
  
  
  RESULTS
  A total of 2164 genes and transcripts were differentially expressed, accounting for 4.9% of all genes represented on the chip. In addition to iron metabolism related to the innate immunity and the signaling pathways, these differentially expressed genes also involved in the adaptive immunity, mainly including the genes associated with B and T cells activation, proliferation and expansion. Transcription profiles of Th17-related transcription factors, cytokines and cytokine receptors during 35 days post-vaccination implied that Th17 cells be activated in bath-vaccinated zebrafish.
  
  
  CONCLUSIONS
  The transcriptome profiling with microarray revealed the Th17-like immune response to bath-vaccination with the live attenuated V. anguillarum in zebrafish.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Fei
authors.forename: Haizhen
authors.initials: H
authors.lastname: Wu
authors.forename: Minjun
authors.initials: M
authors.lastname: Yang
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
authors.forename: Qiyao
authors.initials: Q
authors.lastname: Wang
authors.forename: Yuanxing
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D056704:Adaptive Immunity / Q000235:genetics; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D016207:Cytokines / Q000235:genetics; D020869:Gene Expression Profiling; D011956:Receptors, Cell Surface / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D058504:Th17 Cells / Q000276:immunology*; D014157:Transcription Factors / Q000235:genetics; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000276:immunology; D014733:Vibrio / Q000276:immunology*; D015027:Zebrafish / Q000235:genetics* / Q000276:immunology*
chemical_list: D001428:Bacterial Vaccines; D016207:Cytokines; D011956:Receptors, Cell Surface; D014157:Transcription Factors; D014613:Vaccines, Attenuated
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