title: The DEAD-box RNA helicase DDX5 acts as a positive regulator of Japanese encephalitis virus replication by binding to viral 3' UTR.
abstract: Japanese encephalitis virus (JEV), one of the causes for epidemic encephalitis, belongs to the family of Flaviviridae. In this study, we demonstrated that cellular DEAD-box RNA helicase DDX5 plays an important role in JEV replication. The knockdown of DDX5 was able to decrease JEV replication, and overexpression of DDX5 mutants lacking the helicase activity also reduced JEV replication, suggesting the helicase activity is essential for JEV replication. DDX5 knockdown did not affect virus assembly and release. GST-pulldown and co-immunoprecipitation experiments demonstrated that DDX5 could interact with JEV core protein, non-structural protein 3 (NS3) and 5 (NS5-MTase and NS5-RdRp domains). Meanwhile, we also confirmed that DDX5 interacts with these viral proteins during JEV infection. Confocal microscopy analysis showed that endogenous DDX5 is recruited to the cytoplasm and colocalizes with these viral proteins and viral RNA. RNA-pulldown experiment showed that DDX5 only binds to the JEV 3' untranslated region (UTR). Finally, we confirmed the role of DDX5 in JEV RNA replication using JEV-replicon system. In conclusion, we identified DDX5 as a positive regulator for JEV replication.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing, Jiangsu Province 210095, China; Shandong Binzhou Animal Science and Veterinary Medicine Institute, Binzhou 256600, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.forename: Ling-ling
authors.initials: LL
authors.lastname: Ge
authors.forename: Peng-peng
authors.initials: PP
authors.lastname: Li
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Ming-xia
authors.initials: MX
authors.lastname: Sun
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.forename: Hassan
authors.initials: H
authors.lastname: Ishag
authors.forename: Dong-dong
authors.initials: DD
authors.lastname: Di
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Shen
authors.forename: Wei-Xing
authors.initials: WX
authors.lastname: Fan
authors.forename: Xiang
authors.initials: X
authors.lastname: Mao
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002460:Cell Line; D002498:Centrifugation; D006224:Cricetinae; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D004664:Encephalitis Virus, Japanese / Q000502:physiology*; D055785:Gene Knockdown Techniques; D054884:Host-Pathogen Interactions; D006801:Humans; D047468:Immunoprecipitation; D018613:Microscopy, Confocal; D025941:Protein Interaction Mapping; D012367:RNA, Viral / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D020413:3' Untranslated Regions; D012367:RNA, Viral; C506749:Ddx5 protein, human; D053487:DEAD-box RNA Helicases
keywords: DEAD-box RNA helicase DDX5; Japanese encephalitis virus; Non-structural protein; Replication; Untranslated region
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