title: Probiotic features of two oral Lactobacillus isolates.
abstract: In this study, we checked lactobacilli strains of human origin for their potential as probiotic. Samples were collected from oral mucosa of 16 healthy individuals, out of which twenty isolates were obtained and two of them were selected and identified as Lactobacillus plantarum (G1) and L. casei (G3). Both isolates exhibited antagonistic action towards pathogenic microorganisms such as Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella abony, and Clostridium sporogenes, but not on the growth of Candida albicans. The bacteriocin activity against Staphylococcus aureus ATCC 6358-P was shown only by L. plantarum G1. Moreover, the isolates G1 and G3 showed good viability in the acid gastric environment and in the gut environment containing bovine bile salts. The viability of G1 and G3 isolates in the gastrointestinal tract, and the adhesion to the intestinal mucosa were also confirmed in vivo. The biochemical tests of blood samples revealed lower levels of serum triglycerides and cholesterol, as well as reduced activity of alkaline phosphatase in all lactobacilli-treated Wistar rats, compared to control ones. No toxicity for NMRI Ham mice was observed. According to our experimental results, these findings imply that L. plantarum G1 and L. casei G3 could be characterized as potential probiotics.
authors.affiliation: Galenika a.d. Institute for R&D, Batajnički drum bb , 11080 Belgrade , Serbia.
authors.forename: Gordana
authors.initials: G
authors.lastname: Zavisic
authors.forename: Sasa
authors.initials: S
authors.lastname: Petricevic
authors.forename: Zeljka
authors.initials: Z
authors.lastname: Radulovic
authors.forename: Jelena
authors.initials: J
authors.lastname: Begovic
authors.forename: Natasa
authors.initials: N
authors.lastname: Golic
authors.forename: Ljubisa
authors.initials: L
authors.lastname: Topisirovic
authors.forename: Ivana
authors.initials: I
authors.lastname: Strahinic
keywords: Lactobacillus; antimicrobial activity; hipolypemic effect; probiotics
references.citation: Med J Aust. 2008 Mar 3;188(5):304-8
references.pmid: 18312197
references.citation: J Dairy Sci. 1998 Aug;81(8):2103-10
references.pmid: 9749373
references.citation: Appl Environ Microbiol. 2002 Sep;68(9):4689-93
references.pmid: 12200334
references.citation: J Appl Microbiol. 2003;94(3):449-55
references.pmid: 12588553
references.citation: J Oral Microbiol. 2009 May 01;1:
references.pmid: 21523212
references.citation: J Dairy Sci. 2005 Jan;88(1):55-66
references.pmid: 15591367
references.citation: Clin Chem. 1982 Oct;28(10):2077-80
references.pmid: 6812986
references.citation: Appl Environ Microbiol. 2008 Aug;74(16):4985-96
references.pmid: 18539818
references.citation: Rev Med Suisse. 2007 Oct 17;3(129):2350-4
references.pmid: 18018827
references.citation: Microb Ecol. 2010 Oct;60(3):655-64
references.pmid: 20625713
references.citation: Oral Microbiol Immunol. 2005 Dec;20(6):354-61
references.pmid: 16238595
references.citation: J Food Prot. 2004 Dec;67(12):2727-34
references.pmid: 15633678
references.citation: J Appl Microbiol. 2007 Dec;103(6):2142-52
references.pmid: 18045397
references.citation: Appl Environ Microbiol. 2009 Apr;75(7):1961-9
references.pmid: 19201979
references.citation: Appl Environ Microbiol. 2001 Aug;67(8):3450-4
references.pmid: 11472918
references.citation: Clin Chem. 1974 Apr;20(4):470-5
references.pmid: 4818200
references.citation: Appl Microbiol Biotechnol. 2009 Aug;84(2):341-7
references.pmid: 19444443
references.citation: PLoS One. 2009 Dec 01;4(12):e8099
references.pmid: 19956615
references.citation: Genetika. 2010 Dec;46(12):1589-97
references.pmid: 21434412
references.citation: Indian J Med Sci. 2006 Sep;60(9):361-70
references.pmid: 16940685
references.citation: J Appl Microbiol. 1998 Jul;85(1):88-94
references.pmid: 9721659
references.citation: Microbiology (Reading). 2006 Nov;152(Pt 11):3185-3196
references.pmid: 17074890
references.citation: Open Microbiol J. 2008;2:38-48
references.pmid: 19088910
references.citation: J Dairy Sci. 2000 Dec;83(12):2747-52
references.pmid: 11132841
references.citation: Clin Chem. 1983 Mar;29(3):538-42
references.pmid: 6825269
references.citation: Am J Clin Nutr. 2002 Dec;76(6):1249-55
references.pmid: 12450890
references.citation: Am J Clin Nutr. 2001 Feb;73(2 Suppl):465S-470S
references.pmid: 11157359