title: Biogenic silver nanoparticles using Rhinacanthus nasutus leaf extract: synthesis, spectral analysis, and antimicrobial studies.
abstract: Nanotechnology is gaining momentum due to its ability to transform metals into nanoparticles. The synthesis, characterization, and applications of biologically synthesized nanomaterials have become an important branch of nanotechnology. Plant extracts are a cost-effective, ecologically friendly, and efficient alternative for the large-scale synthesis of nanoparticles. In this study, silver nanoparticles (AgNps) were synthesized using Rhinacanthus nasutus leaf extract. After exposing the silver ions to the leaf extract, the rapid reduction of silver ions led to the formation of AgNps in solution. The synthesis was confirmed by ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, and transmission electron microscopy. The in vitro antimicrobial activity of the AgNps synthesized using R. nasutus leaf extract was investigated against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, Escherichia coli, Aspergillus niger, and Aspergillus flavus using a disc diffusion method. The AgNps showed potential activity against all of the bacterial strains and fungal colonies, indicating that R. nasutus has the potential to be used in the development of value-added products in the biomedical and nanotechnology-based industries.
authors.affiliation: Human Genome Center, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.
authors.forename: Visweswara Rao
authors.initials: VR
authors.lastname: Pasupuleti
authors.forename: T N V
authors.initials: TN
authors.lastname: Prasad
authors.forename: Rayees Ahmad
authors.initials: RA
authors.lastname: Shiekh
authors.forename: Satheesh Krishna
authors.initials: SK
authors.lastname: Balam
authors.forename: Ganapathi
authors.initials: G
authors.lastname: Narasimhulu
authors.forename: Cirandur Suresh
authors.initials: CS
authors.lastname: Reddy
authors.forename: Ismail
authors.initials: I
authors.lastname: Ab Rahman
authors.forename: Siew Hua
authors.initials: SH
authors.lastname: Gan
mesh_terms: D027901:Acanthaceae; D000890:Anti-Infective Agents / Q000138:chemical synthesis / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D001688:Biological Products / Q000138:chemical synthesis / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry* / Q000648:ultrastructure; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D001688:Biological Products; D010936:Plant Extracts; D012834:Silver
keywords: R. nasutus; TEM; antimicrobial activities; silver nanoparticles
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