title: Development of a polymerase chain reaction assay for the detection of pseudorabies virus in clinical samples.
abstract: Aujeszky's disease, also known as pseudorabies causes severe economic losses in swine industry and affects the pig husbandry all over the world. The conventional diagnostic procedure is time-consuming and false-negative results may occur in submissions from latently infected animals. The development, optimization and evaluation of a polymerase chain reaction (PCR) assay are presented for the diagnosis of pseudorabies infection. This assay was based on the amplification of a highly conserved viral gD gene fragment. PCR products of the expected size were obtained from PRV strains. Non-specific reactions were not observed when a related herpesvirus, other porcine DNA genome viruses and uninfected cells were used to assess PCR. The analytical sensitivity of the test was estimated to be 1.34 TCID50/ 50 uL. The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases. A rapid, sensitive and specific PCR-based diagnostic assay to detect pseudorabies virus in clinical samples is provided.
authors.affiliation: Grupo de Virología, Centro Nacional de Sanidad Agropecuaria. San José de las Lajas , La Habana , Cuba.
authors.forename: Lester J
authors.initials: LJ
authors.lastname: Pérez
authors.forename: Heidy Díaz
authors.initials: HD
authors.lastname: de Arce
keywords: Aujeszky’s disease; PCR assay; pseudorabies infection
references.citation: J Virol. 2001 Jan;75(1):469-79
references.pmid: 11119615
references.citation: J Virol Methods. 2007 Jan;139(1):39-43
references.pmid: 17034871
references.citation: J Wildl Dis. 2003 Jul;39(3):567-75
references.pmid: 14567217
references.citation: Can J Comp Med. 1976 Jul;40(3):252-6
references.pmid: 187299
references.citation: DNA Cell Biol. 1991 Apr;10(3):233-8
references.pmid: 2012681
references.citation: Vet Res Commun. 2005 Apr;29(3):263-9
references.pmid: 15736858
references.citation: Vet Res. 2007 Mar-Apr;38(2):229-41
references.pmid: 17257571
references.citation: Vet Microbiol. 1997 Apr;55(1-4):37-47
references.pmid: 9220595
references.citation: Vet Res. 2000 Jan-Feb;31(1):99-115
references.pmid: 10726640
references.citation: Vet Microbiol. 1997 Apr;55(1-4):13-27
references.pmid: 9220593
references.citation: Can J Vet Res. 1997 Apr;61(2):157-60
references.pmid: 9114968
references.citation: Vet Rec. 2003 Sep 13;153(11):340
references.pmid: 14516122
references.citation: Vaccine. 2007 Jul 26;25(30):5524-30
references.pmid: 17229496