title: Adherence assays and Slime production of Vibrio alginolyticus and Vibrio parahaemolyticus.
abstract: In this study we investigated the phenotypic slime production of Vibrio alginolyticus and Vibrio parahaemolyticus strains, food-borne pathogens, using a Congo red agar plate assay. Furthermore, we studied their ability to adhere to abiotic surfaces and Vero cells line. Our results showed that only V. alginolyticus ATCC 17749 was a slime-producer developing almost black colonies on Congo red agar plate. Adherence to glace tube showed that all V. alginolyticus strains were more adherent than V. parahaemolyticus. Only V. alginolyticus ATCC 17749 was found to be able to form biofilm on polystyrene microplate wells (OD570 = 0.532). Adherence to Vero cells showed that all tested strains were non adherent after 30 min, however after 60 min all the studied strains become adherent. The percentage of adherence ranged from1.23% to 4.66%.
authors.affiliation: Laboratoire d'Analyse, Traitement et Valorisation des Polluants de l'Environnement et des Produits. Faculté de Pharmacie Rue Avicenne , Monastir 5000 , Tunisia ; Unité de fermentation et de développement de vaccins virologiques. Institut Pasteur de Tunis , 13 place Pasteur. 1002 , Tunisia.
authors.forename: Fethi
authors.initials: F
authors.lastname: Ben Abdallah
authors.forename: Kamel
authors.initials: K
authors.lastname: Chaieb
authors.forename: Tarek
authors.initials: T
authors.lastname: Zmantar
authors.forename: Hela
authors.initials: H
authors.lastname: Kallel
authors.forename: Amina
authors.initials: A
authors.lastname: Bakhrouf
keywords: Vero cells; Vibrio; abiotic surface; adherence; slime production
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