title: Identification of FAM96B as a novel prelamin A binding partner.
abstract: Prelamin A accumulation causes nuclear abnormalities, impairs nuclear functions, and eventually promotes cellular senescence. However, the underlying mechanism of how prelamin A promotes cellular senescence is still poorly understood. Here we carried out a yeast two-hybrid screen using a human skeletal muscle cDNA library to search for prelamin A binding partners, and identified FAM96B as a prelamin A binding partner. The interaction of FAM96B with prelamin A was confirmed by GST pull-down and co-immunoprecipitation experiments. Furthermore, co-localization experiments by fluorescent confocal microscopy revealed that FAM96B colocalized with prelamin A in HEK-293 cells. Taken together, our data demonstrated the physical interaction between FAM96B and prelamin A, which may provide some clues to the mechanisms of prelamin A in premature aging.
authors.affiliation: Institute of Aging Research, Guangdong Medical College, Dongguan 523808, PR China; Institute of Biochemistry & Molecular Biology, Guangdong Medical College, Zhanjiang 524023, PR China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan 523808, PR China.
authors.forename: Xing-Dong
authors.initials: XD
authors.lastname: Xiong
authors.forename: Junwen
authors.initials: J
authors.lastname: Wang
authors.forename: Huiling
authors.initials: H
authors.lastname: Zheng
authors.forename: Xia
authors.initials: X
authors.lastname: Jing
authors.forename: Zhenjie
authors.initials: Z
authors.lastname: Liu
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Zhou
authors.forename: Xinguang
authors.initials: X
authors.lastname: Liu
mesh_terms: D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism*; D016922:Cellular Senescence; D057809:HEK293 Cells; D006801:Humans; D034904:Lamin Type A; D008667:Metalloproteins; D009687:Nuclear Proteins / Q000032:analysis / Q000378:metabolism*; D011371:Progeria / Q000378:metabolism; D011485:Protein Binding; D060066:Protein Interaction Maps; D011498:Protein Precursors / Q000032:analysis / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: C569498:CIAO2B protein, human; D002352:Carrier Proteins; D034904:Lamin Type A; D008667:Metalloproteins; D009687:Nuclear Proteins; D011498:Protein Precursors; C090764:prelamin A
keywords: CIA; Cellular senescence; FAM96B; HGPS; Hutchinson–Gilford progeria syndrome; Prelamin A; family with sequence similarity 96 member B; the cytoplasmic Fe–S assembly