title: Analysis of productive binding modes in the human chitotriosidase.
abstract: Human chitotriosidase (HCHT) is a family 18 chitinase that is an innate part of the immune system. We have mapped preferred productive binding modes of chito-oligosaccharide substrates to HCHT and the data show that HCHT has strong binding affinity in the +3 subsite. Moreover, HCHT shows anomer-specific binding affinities in subsites +2 and +3. These features could endorse HCHT with higher endo-activity and a higher transglycosylation potential.
authors.affiliation: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, PO 5003, N-1432 Ås, Norway.
authors.forename: Kristine Bistrup
authors.initials: KB
authors.lastname: Eide
authors.forename: Anne Rita
authors.initials: AR
authors.lastname: Lindbom
authors.forename: Vincent G H
authors.initials: VG
authors.lastname: Eijsink
authors.forename: Anne Line
authors.initials: AL
authors.lastname: Norberg
authors.forename: Morten
authors.initials: M
authors.lastname: Sørlie
mesh_terms: D001665:Binding Sites; D002686:Chitin / Q000378:metabolism; D006596:Hexosaminidases / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D009844:Oligosaccharides / Q000378:metabolism; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D013379:Substrate Specificity
chemical_list: D009844:Oligosaccharides; D002686:Chitin; D006596:Hexosaminidases; C086400:chitotriosidase
keywords: Chitotriosidase; Endo-activity; Human chitinase; Transglycosylation