title: Clinicopathological significance of SIRT1 and p300/CBP expression in gastroesophageal junction (GEJ) cancer and the correlation with E-cadherin and MLH1.
abstract: SIRT1 and p300/CBP, which are considered to be essential histone deacetylases and acetyltransferases, are also considered to be relative to tumorigenesis because they modulate the expression of several tumor suppressor genes. Therefore, this study investigated the expression of SIRT1 and p300/CBP in gastroesophageal junction (GEJ) cancer and their correlation with E-cadherin and MLH1 in order to explore the clinicopathological significance of SIRT1 and p300/CBP expression and their possible effects involving E-cadherin and MLH1 expression. Tissue microarray technique and immunohistochemical stains were applied to evaluate the SIRT1, p300/CBP, E-cadherin, and MLH1 expression in 176 GEJ cancer tissues and 32 normal GEJ region tissues. The results showed that the over-expression of SIRT1 was associated with a higher number of metastasis lymph nodes, more advanced staging, and shorter mean survival time. SIRT1 and p300/CBP were negatively and positively correlated with the expression of E-cadherin and MLH1, respectively, in the cancer cases. These results indicated a possible effect of SIRT1 and p300/CBP involved in regulating the expression of E-cadherin and MLH1, thus participating in the tumor progression of GEJ cancer.
authors.affiliation: Department of Pathology, Affiliated Zhongda Hospital Southeast University, 87 Dingjiaqiao, Nanjing, Jiangsu 210009, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Zhang
authors.forename: Qin
authors.initials: Q
authors.lastname: Huang
authors.forename: Xiang-Shan
authors.initials: XS
authors.lastname: Fan
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: An-Ning
authors.initials: AN
authors.lastname: Feng
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis / Q000096:biosynthesis*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis; D015820:Cadherins / Q000032:analysis / Q000096:biosynthesis*; D002277:Carcinoma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D004943:Esophagogastric Junction / Q000378:metabolism / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D000070957:MutL Protein Homolog 1; D009687:Nuclear Proteins / Q000032:analysis / Q000096:biosynthesis*; D011379:Prognosis; D016016:Proportional Hazards Models; D056564:Sirtuin 1 / Q000032:analysis / Q000096:biosynthesis*; D013274:Stomach Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D046888:Tissue Array Analysis; D050880:p300-CBP Transcription Factors / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; D015820:Cadherins; C501486:MLH1 protein, human; D009687:Nuclear Proteins; D050880:p300-CBP Transcription Factors; C447939:SIRT1 protein, human; D056564:Sirtuin 1; D000070957:MutL Protein Homolog 1
keywords: GEJ Cancer; Prognosis; SIRT1; p300/CBP