title: Hydrolysis of ozone pretreated energy grasses for optimal fermentable sugar production.
abstract: Ozonated energy grass varieties were enzymatically hydrolyzed to establish process parameters for maximum fermentable sugar production. Conditions for ozonolysis were selected on the basis of maximum delignification and glucan retention after pretreatment. To study the effect of lignin degradation products generated during ozonolysis on cellulolytic enzymes, hydrolysis was carried out for washed and unwashed pretreated solids. Washing the solids significantly (p<0.05) enhanced glucan conversion from 34.3% to 100% while delivering glucose yields of 146.2-431.9 mg/g biomass. Highest fermentable sugars were produced when grasses were ozonated for maximum delignification and washed solids were hydrolyzed using 0.1g/g Cellic® CTec2. In a comparative study on alkaline pretreatment with 1% NaOH for 60 min, Saccharum arundinaceum exhibited the highest glucan conversion with maximum sugar production of 467.9 mg/g. Although ozonolysis is an effective and environmentally friendly technique for cellulosic sugar production, process optimization is needed to ascertain economic feasibility of the process.
authors.affiliation: Department of Biological and Agricultural Engineering, Campus Box 7625, North Carolina State University (NCSU), Raleigh, NC 27695, USA.
authors.forename: Anushadevi
authors.initials: A
authors.lastname: Panneerselvam
authors.forename: Ratna R
authors.initials: RR
authors.lastname: Sharma-Shivappa
authors.forename: Praveen
authors.initials: P
authors.lastname: Kolar
authors.forename: Debra A
authors.initials: DA
authors.lastname: Clare
authors.forename: Thomas
authors.initials: T
authors.lastname: Ranney
mesh_terms: D000143:Acids / Q000737:chemistry; D056804:Biofuels; D018533:Biomass; D002241:Carbohydrates / Q000096:biosynthesis*; D005285:Fermentation / Q000187:drug effects*; D005947:Glucose / Q000096:biosynthesis; D006868:Hydrolysis / Q000187:drug effects; D008031:Lignin / Q000032:analysis; D010126:Ozone / Q000494:pharmacology*; D006109:Poaceae / Q000187:drug effects / Q000378:metabolism*; D012972:Sodium Hydroxide / Q000494:pharmacology; D012995:Solubility; D014990:Xylans / Q000378:metabolism; D014994:Xylose / Q000096:biosynthesis
chemical_list: D000143:Acids; D056804:Biofuels; D002241:Carbohydrates; D014990:Xylans; D012972:Sodium Hydroxide; D010126:Ozone; D008031:Lignin; D014994:Xylose; D005947:Glucose
keywords: Alkali pretreatment; Enzymatic hydrolysis; Miscanthus; Optimization; Saccharum