title: FoxP3 genetic variants and risk of non-small cell lung cancer in the Chinese Han population.
abstract: CD4(+)CD25(+) regulatory T cell-mediated immunosuppression is one of the crucial mechanisms that tumor cells use to evade the immune system. The forkhead box P3 (FoxP3) gene regulates regulatory T-cell development and function and may modulate the susceptibility to non-small cell lung cancer (NSCLC). Because a single nucleotide polymorphism (SNP) within the FoxP3 gene (rs3761548 in the promoter region) is associated with susceptibility to Graves' disease, this study detected rs3761548 in a hospital-based case-control study. A total of 192 NSCLC patients and 259 healthy subjects were recruited for the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of FoxP3 SNP. The data showed that the A allele of rs3761548 significantly increased NSCLC risk (P=0.000, OR=2.32, 95%CI=1.736-3.102). The AC genotype, AA genotype, and the combined A variant genotype (AA+AC) were also associated with a higher risk of NSCLC (OR [95%CI]=2.147[1.419-3.247], 4.413[2.359-8.255], and 2.563[1.746-3.761], respectively). Moreover, a significantly higher frequency of AA+AC genotype was observed in patients with stage II NSCLC (OR, 2.053; 95%CI, 1.033-4.078). In conclusion, the data from the current study demonstrated for the first time the association of the FoxP3 SNP with a risk of developing NSCLC in the Chinese Han population.
authors.affiliation: Department of Respiratory Medicine, West China Hospital of Sichuan University, Chengdu 610041, China.
authors.forename: Yan-Qi
authors.initials: YQ
authors.lastname: He
authors.forename: Qiao
authors.initials: Q
authors.lastname: Bo
authors.forename: Wei
authors.initials: W
authors.lastname: Yong
authors.forename: Zhi-Xin
authors.initials: ZX
authors.lastname: Qiu
authors.forename: Ya-Lun
authors.initials: YL
authors.lastname: Li
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics / Q000706:statistics & numerical data; D002289:Carcinoma, Non-Small-Cell Lung / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics*; D016022:Case-Control Studies; D015331:Cohort Studies; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008175:Lung Neoplasms / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors
keywords: CI; Cancer risk; FoxP3; Lung cancer; NSCLC; OR; PCR-RFLP; SCC; SNP; Single nucleotide polymorphism; Tregs; confidence intervals; forkhead box P3; non-small cell lung cancer; odds ratio; polymerase chain reaction–restriction fragment length polymorphism; regulatory T cells; single nucleotide polymorphism; squamous cell carcinoma