title: Comparative analysis of volatile metabolomics signals from melanoma and benign skin: a pilot study.
abstract: The analysis of volatile organic compounds (VOC) as biomarkers of cancer is both promising and challenging. In this pilot study, we used an untargeted approach to compare volatile metabolomic signatures of melanoma and matched control non-neoplastic skin from the same patient. VOC from fresh (non-fixed) biopsied tissue were collected using the headspace solid phase micro extraction method (HS SPME) and analyzed by gas chromatography and mass spectrometry (GCMS). We applied the XCMS analysis platform and MetaboAnalyst software to reveal many differentially expressed metabolic features. Our analysis revealed increased levels of lauric acid (C12:0) and palmitic acid (C16:0) in melanoma. The identity of these compounds was confirmed by comparison with chemical standards. Increased levels of these fatty acids are likely to be a consequence of up-regulated de novo lipid synthesis, a known characteristic of cancer. Increased oxidative stress is likely to cause an additional increase in lauric acid. Implementation of this study design on larger number of cases will be necessary for the future metabolomics biomarker discovery applications.
authors.affiliation: Molecular and Cellular Pharmacology, University of Miami, Miami, FL USA.
authors.forename: T
authors.initials: T
authors.lastname: Abaffy
authors.affiliation: Division of Surgical Oncology, DeWitt Daughtry Department of Surgery, University of Miami, Miami, FL USA.
authors.forename: M G
authors.initials: MG
authors.lastname: Möller
authors.affiliation: Marine and Atmospheric Chemistry, Rosenstiel School of Marine and Atmospheric Science, University of Miami, Miami, FL USA.
authors.forename: D D
authors.initials: DD
authors.lastname: Riemer
authors.affiliation: Department of Pathology, University of Miami, Miami, FL USA.
authors.forename: C
authors.initials: C
authors.lastname: Milikowski
authors.affiliation: Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI USA.
authors.forename: R A
authors.initials: RA
authors.lastname: DeFazio
keywords: GCMS; Metabolites; Palmitic acid; Skin cancer; Volatile organic compounds
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