title: An improved liquid chromatography-tandem mass spectrometric method to quantify formoterol in human urine.
abstract: Formoterol is a new threshold substance in the prohibited list 2012 according to World Anti-Doping Agency. Extracted by ethyl acetate using formoterol-D6 as internal standard, formoterol underwent a constant flow rate gradient elution separation in reversed-phase liquid chromatography. Subsequently, mass spectrometry analysis was conducted by tandem mass spectrometry in the multiple reaction monitoring mode. According to the proposed method, a calibration curve was constructed in the range of 0.2-500 ng/mL (r(2) = 1) with a limit of quantification 0.2 ng/mL. The mean extracted recovery assessed at three different concentrations (1, 30 and 100 ng/mL) was more than 80%. The method was validated by the analysis of three quality control samples from World Association of Anti-Doping Scientists. In conclusion, the developed and validated method was sensitive, accurate and precise for the quantiﬁcation of formoterol in human urine for doping control purposes.
authors.affiliation: National Anti-Doping Laboratory, China Anti-Doping Agency, 1st Anding Road, ChaoYang District, Beijing 100029, PR China.
authors.forename: Genye
authors.initials: G
authors.lastname: He
authors.affiliation: National Anti-Doping Laboratory, China Anti-Doping Agency, 1st Anding Road, ChaoYang District, Beijing 100029, PR China.
authors.forename: Jianghai
authors.initials: J
authors.lastname: Lu
authors.affiliation: National Anti-Doping Laboratory, China Anti-Doping Agency, 1st Anding Road, ChaoYang District, Beijing 100029, PR China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Wang
authors.affiliation: National Anti-Doping Laboratory, China Anti-Doping Agency, 1st Anding Road, ChaoYang District, Beijing 100029, PR China xuyouxuan@chinada.cn.
authors.forename: Youxuan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: National Anti-Doping Laboratory, China Anti-Doping Agency, 1st Anding Road, ChaoYang District, Beijing 100029, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wu
authors.affiliation: National Anti-Doping Laboratory, China Anti-Doping Agency, 1st Anding Road, ChaoYang District, Beijing 100029, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Dong
authors.affiliation: National Anti-Doping Laboratory, China Anti-Doping Agency, 1st Anding Road, ChaoYang District, Beijing 100029, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.affiliation: National Anti-Doping Laboratory, China Anti-Doping Agency, 1st Anding Road, ChaoYang District, Beijing 100029, PR China.
authors.forename: Zhenwen
authors.initials: Z
authors.lastname: He
authors.affiliation: National Anti-Doping Laboratory, China Anti-Doping Agency, 1st Anding Road, ChaoYang District, Beijing 100029, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.affiliation: National Anti-Doping Laboratory, China Anti-Doping Agency, 1st Anding Road, ChaoYang District, Beijing 100029, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yuan
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D004983:Ethanolamines / Q000652:urine*; D000068759:Formoterol Fumarate; D006801:Humans; D015203:Reproducibility of Results; D015813:Substance Abuse Detection / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004983:Ethanolamines; D000068759:Formoterol Fumarate