title: [Visual detection of HIV-1 by reverse transcription loop-mediated isothermal amplification with the hydroxynaphthol blue dye].
abstract: OBJECTIVE
  A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of HIV-1.
  
  
  METHODS
  RT-LAMP primers were designed according to conservative sequences of HIV-1 gag gene, and their sensitivity and specificity were evaluated by the established RT-LAMP protocol with the addition of the hydroxynaphthol blue (HNB) dye prior to amplification. The performance of RT-LAMP on clinical samples was compared with real-time reverse transcription PCR(qRT-PCR).
  
  
  RESULTS
  The RT-LAMP assay showed a high specificity, and its detection limit was 1000 copies RNA per tube. The sensitivity and specificity of this method using 43 clinical samples were 94.6% and 100%, respectively,in comparison with those of qRT-PCR.
  
  
  CONCLUSIONS
  RT-LAMP assay using hydroxynaphthol blue dye does not need expensive instruments, and offer an alternative for the rapid detection of HIV-1 with the potential to be applied in field diagnosis.
authors.forename: Ya-Lan
authors.initials: YL
authors.lastname: Zeng
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Zhang
authors.forename: Kai
authors.initials: K
authors.lastname: Nei
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Meng-Jie
authors.initials: MJ
authors.lastname: Yang
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Shen
authors.forename: Ji
authors.initials: J
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Ma
mesh_terms: D015497:HIV-1 / Q000302:isolation & purification*; D009282:Naphthalenesulfonates; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D048348:Reverse Transcription; D012680:Sensitivity and Specificity
chemical_list: D009282:Naphthalenesulfonates; C540250:trisodium 3-hydroxy-4-((2Z)-2-(2-oxo-4-sulfonatonaphthalen-1-ylidene)hydrazinyl)naphthalene-2,7-disulfonate