title: Differential expression of miR-195 in esophageal squamous cell carcinoma and miR-195 expression inhibits tumor cell proliferation and invasion by targeting of Cdc42.
abstract: MicroRNAs (miRNA) have played an important role in carcinogenesis. In this study, Agilent miRNA microarray was used to identify differentially expressed miRNAs in esophageal squamous cell carcinoma (ESCC) tissues and miR-195 was downregulated in ESCC compared with normal esophageal tissues. Moreover, Cdc42 was confirmed as target gene of miR-195. Ectopic expression of miR-195 in ESCC cells significantly downregulated Cdc42 by directly binding its 3' untranslated regions, and induced G1 cell cycle arrest, leading to a significant decrease in cell growth, migration, and invasion in vitro. Therefore, our findings demonstrated that miR-195 may act as a tumor suppressor in ESCC by targeting Cdc42.
authors.affiliation: The First Affiliated Hospital of Nanjing Medical University, Nanjing, China; Xinyu People's Hospital, Jiangxi, China.
authors.forename: Min-gen
authors.initials: MG
authors.lastname: Fu
authors.forename: Shuo
authors.initials: S
authors.lastname: Li
authors.forename: Ting-ting
authors.initials: TT
authors.lastname: Yu
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Qian
authors.forename: Ri-sheng
authors.initials: RS
authors.lastname: Cao
authors.forename: Hong
authors.initials: H
authors.lastname: Zhu
authors.forename: Bin
authors.initials: B
authors.lastname: Xiao
authors.forename: Chun-hua
authors.initials: CH
authors.lastname: Jiao
authors.forename: Na-na
authors.initials: NN
authors.lastname: Tang
authors.forename: Jing-jing
authors.initials: JJ
authors.lastname: Ma
authors.forename: Jie
authors.initials: J
authors.lastname: Hua
authors.forename: Wei-feng
authors.initials: WF
authors.lastname: Zhang
authors.forename: Hong-jie
authors.initials: HJ
authors.lastname: Zhang
authors.forename: Rui-hua
authors.initials: RH
authors.lastname: Shi
mesh_terms: D020413:3' Untranslated Regions; D000368:Aged; D016203:CDC2 Protein Kinase; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019926:Cyclin B / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D018844:Cyclin-Dependent Kinases; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology*; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; D019926:Cyclin B; C552722:MIRN195 microRNA, human; D035683:MicroRNAs; D016203:CDC2 Protein Kinase; C528899:CDK1 protein, human; D018844:Cyclin-Dependent Kinases
keywords: Cdc42; Esophageal squamous cell carcinoma; Tumor progression; miR-195; miRNA