title: Downregulation of miR-145 contributes to lung adenocarcinoma cell growth to form brain metastases.
abstract: The development of metastases involves the dissociation of cells from the primary tumor, penetrating the basement membrane, invasion and exiting from the vasculature to seed, and finally colonizing in distant tissues. The formation of brain metastasis (BM) in lung adenocarcinoma remains poorly understood. We examined the differential microRNA (miRNA) expression profiles of 5 primary and 3 brain metastatic lung adenocarcinoma samples by Agilent miRNA Microarrays. Five upregulated miRNAs (miRs-9*, -1471, 718, 3656, 720) and 3 downregulated miRNAs (miRs-214, -145 and -23a) were detected. The 4 most significantly deregulated miRNAs (miR-145, miR-214, miR-9* and miR-1471) were validated in the additional 43 samples (35 primary and 8 brain metastatic lung adenocarcinoma samples) using TaqMan quantitative PCR. By functional assay, we found that the expression of miR-145 can regulate the ability of proliferation of A549 and SPC-A1 cells in vitro, but is not related to lymph node metastasis, migration and invasion. These results suggest that miR-145 may have a cell type-specific function and play important roles in the process of BM from lung adenocarcinoma.
authors.affiliation: Biorepository, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Chunyang
authors.initials: C
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Weiwei
authors.initials: W
authors.lastname: Tan
authors.forename: Jianxin
authors.initials: J
authors.lastname: Xue
authors.forename: Zongze
authors.initials: Z
authors.lastname: Yang
authors.forename: You
authors.initials: Y
authors.lastname: Zhang
authors.forename: You
authors.initials: Y
authors.lastname: Lu
authors.forename: Xun
authors.initials: X
authors.lastname: Hu
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D000328:Adult; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology / Q000556:secondary; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008207:Lymphatic Metastasis / Q000235:genetics / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D015854:Up-Regulation
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs
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