title: Effect of aqueous extract of sanweitanxiang powder on calcium homeostasis protein expression in ischemic-reperfusion injury rat heart.
abstract: OBJECTIVE
  To investigate the underlying mechanism of reduced myocardial ischemia-reperfusion (I/R) injury in rats using the traditional Tibetan medicine Sanweitanxiang powder (SWTX).
  
  
  METHODS
  Rats were randomly divided into six groups (n = 10) as follows: (a) propranolol dinitrate control group, given propranolol dinitrate 0.02 g/kg for 10 days before I/R, (b) SWTX with a high dose group, given SWTX 1.5 g/kg for 10 days before I/R, (c) SWTX with a medium dose group, given SWTX 1.25 g/kg for 10 days before I/R, (d) sham group (Sham), in which the rat heart was exposed by pericardiotomy but without I/R, (e) SWTX with a low dose group, given SWTX 1.0 g/kg for 10 days before I/R, and (f) I/R injury group. Rats were intragastrically pretreated with propranolol dinitrate or SWTX. After that, the operation to cause ischemia and reperfusion was conducted. The histopathologic changes of rat hearts were observed by hematoxylin and eosin staining and transmission electron microscopy. Ca2+ homeostasis protein expression was determined by western blot.
  
  
  RESULTS
  After SWTX pretreatment, the development of ultrastructural pathological changes from IR injury was attenuated. A decrease in the expression of B-cell lymphoma 2 associated X protein, and an increase in the expression of B-cell lymphoma 2 were observed. An increased activation of extracellular signal regulated kinases were found. Compared with the sham group, the expression of sarcoplasmic reticulum calcium-ATPase, phospholamban, and calsequestrin were all up-regulated after pretreatment with SWTX.
  
  
  CONCLUSIONS
  The protective mechanism of SWTX pretreatment on myocardial I/R injury might be related to its effect on maintaining the balance of calcium homeostasis in rat heart.
authors.affiliation: Research Center for High Altitude Medicine, Qinghai University, Xining 810001, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yang
authors.forename: Shuna
authors.initials: S
authors.lastname: Zhang
authors.forename: Shicun
authors.initials: S
authors.lastname: Ren
authors.forename: Jinjun
authors.initials: J
authors.lastname: Wang
authors.forename: Haixia
authors.initials: H
authors.lastname: Yun
authors.forename: Sheng
authors.initials: S
authors.lastname: Yong
authors.forename: Dejun
authors.initials: D
authors.lastname: Zhang
authors.forename: Quanyu
authors.initials: Q
authors.lastname: Yang
authors.forename: Yiying
authors.initials: Y
authors.lastname: Kou
authors.forename: Dianxiang
authors.initials: D
authors.lastname: Lu
authors.forename: Rili
authors.initials: R
authors.lastname: Ge
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics* / Q000378:metabolism; D006706:Homeostasis; D006801:Humans; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000235:genetics* / Q000378:metabolism
chemical_list: C506948:Atp2a2 protein, rat; D004365:Drugs, Chinese Herbal; D048049:Extracellular Signal-Regulated MAP Kinases; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D002118:Calcium