title: Further characterization of Tsol-p27 as a diagnostic antigen in sub-Saharan Africa.
abstract: Commercial antigens used to diagnose human neurocysticercosis are obtained from either a soluble parasite extract or a parasite-derived glycoprotein fraction. The aim of the present study was to identify antigenic proteins as potential diagnostic candidates in Mozambique. Soluble proteins from Taenia solium cysticerci were separated by two-dimensional electrophoresis and blotted onto nitrocellulose membranes. Subtracted hybridization was performed with serum samples obtained from patients with neurocysticercosis (NCC) and from a NCC-negative control group. Six antigenic proteins were identified and sequenced by liquid chromatography-mass spectrometry. Among these we found Tsol-p27, which was previously identified as a diagnostic candidate in a study conducted in Nicaragua, Central America. Here, we evaluated Tsol-p27 and the antigen cC1 as potential recombinant diagnostic reagents, and also investigated the localization and partial function of Tsol-p27. Immunoblotting demonstrated that Tsol-p27 was recognized by all 10 serum samples from NCC-positive individuals, whereas cC1 was identified by only five of the 10 positive sera. None of the antigens were recognized by negative control sera. Despite the limited number of serum samples evaluated in this study, the results suggest that Tsol-p27 can be a suitable candidate for diagnosis of human NCC, not only in Central America but also in sub-Saharan Africa.
authors.affiliation: Departamento de Microbiologia, Faculdade de Medicina, Universidade Eduardo Mondlane, Mozambique; Microbiology, Tumor and Cell Biology, Karolinska Institutet, Sweden. Electronic address: noemianha@yahoo.com.br.
authors.forename: Noémia
authors.initials: N
authors.lastname: Nhancupe
authors.forename: Fernando
authors.initials: F
authors.lastname: Salazar-Anton
authors.forename: Emília Virginia
authors.initials: EV
authors.lastname: Noormahomed
authors.forename: Sónia
authors.initials: S
authors.lastname: Afonso
authors.forename: Johan
authors.initials: J
authors.lastname: Lindh
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D015153:Blotting, Western; D016022:Case-Control Studies; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D015801:Helminth Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009073:Mozambique; D020019:Neurocysticercosis / Q000175:diagnosis* / Q000276:immunology; D011817:Rabbits; D013552:Swine; D041201:Taenia solium / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D007106:Immune Sera
keywords: Cysticercosis; Diagnosis; Immunoreactive proteins; Serology; Taenia solium
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