title: Inoculum padronization for the production of cutinase by Fusarium oxysporum.
abstract: Cutinase is a versatile enzyme showing several interesting properties for application in industrial processes. The widespread use of this enzyme depends on the development of an efficient and low-cost production system. One of the most important steps in a fermentation process is the standardization of the inoculum characteristics. In this study, the production of cutinase by Fusarium oxysporum showed a statistically significant relationship with both the inoculum size and the inoculum PDA pH. The greatest activities were 19.1 U/mL at PDA pH 7.0 and 22.72 U/mL using an aliquot of 12.72 × 10(7) spores/mL. The macroscopic characteristics of the colonies of Fusarium oxysporum changed according to the variation of the medium pH, with the best results recorded in those colonies presenting a cotton white aspect.
authors.affiliation: Departamento de Ciência de Alimentos, Faculdade de Engenharia de Alimentos, Universidade do Estado de Campinas , Campinas, SP , Brasil.
authors.forename: Tatiana Fontes
authors.initials: TF
authors.lastname: Pio
authors.forename: Laira Priscila
authors.initials: LP
authors.lastname: Fraga
authors.forename: Gabriela Alves
authors.initials: GA
authors.lastname: Macedo
keywords: Fusarium oxysporum; cutinase; fermentation process; inoculum; macroscopic characteristics
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