title: A map of cell type-specific auxin responses.
abstract: In plants, changes in local auxin concentrations can trigger a range of developmental processes as distinct tissues respond differently to the same auxin stimulus. However, little is known about how auxin is interpreted by individual cell types. We performed a transcriptomic analysis of responses to auxin within four distinct tissues of the Arabidopsis thaliana root and demonstrate that different cell types show competence for discrete responses. The majority of auxin-responsive genes displayed a spatial bias in their induction or repression. The novel data set was used to examine how auxin influences tissue-specific transcriptional regulation of cell-identity markers. Additionally, the data were used in combination with spatial expression maps of the root to plot a transcriptomic auxin-response gradient across the apical and basal meristem. The readout revealed a strong correlation for thousands of genes between the relative response to auxin and expression along the longitudinal axis of the root. This data set and comparative analysis provide a transcriptome-level spatial breakdown of the response to auxin within an organ where this hormone mediates many aspects of development.
authors.affiliation: 1] Biology Department, Center for Genomics and Systems Biology, New York University, New York, NY, USA [2] Department of Cell and Developmental Biology, UCSD, La Jolla, CA, USA.
authors.forename: Bastiaan O R
authors.initials: BO
authors.lastname: Bargmann
authors.forename: Steffen
authors.initials: S
authors.lastname: Vanneste
authors.forename: Gabriel
authors.initials: G
authors.lastname: Krouk
authors.forename: Tal
authors.initials: T
authors.lastname: Nawy
authors.forename: Idan
authors.initials: I
authors.lastname: Efroni
authors.forename: Eilon
authors.initials: E
authors.lastname: Shani
authors.forename: Goh
authors.initials: G
authors.lastname: Choe
authors.forename: Jiří
authors.initials: J
authors.lastname: Friml
authors.forename: Dominique C
authors.initials: DC
authors.lastname: Bergmann
authors.forename: Mark
authors.initials: M
authors.lastname: Estelle
authors.forename: Kenneth D
authors.initials: KD
authors.lastname: Birnbaum
mesh_terms: D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000494:pharmacology*; D018519:Meristem / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D009928:Organ Specificity; D018517:Plant Roots / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D059467:Transcriptome
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids
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