title: Multiplex ligation-dependent probe amplification (MLPA) for ultrasensitive multiplexed microRNA detection using ribonucleotide-modified DNA probes.
abstract: Using the ribonucleotide-modified DNA probes, multiple microRNAs can be simultaneously detected in one ligation-based PCR reaction. As low as 0.2 fM microRNA can be accurately detected with high specificity.
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province, P. R. China. liuch@hbu.edu.cn lzpbd@hbu.edu.cn.
authors.forename: Pengbo
authors.initials: P
authors.lastname: Zhang
authors.forename: Ye
authors.initials: Y
authors.lastname: Liu
authors.forename: Yajing
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chenghui
authors.initials: C
authors.lastname: Liu
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhengping
authors.initials: Z
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D001681:Biological Assay; D015342:DNA Probes / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D035683:MicroRNAs / Q000737:chemistry* / Q000235:genetics; D060885:Multiplex Polymerase Chain Reaction; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction; D012265:Ribonucleotides / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D035683:MicroRNAs; D012265:Ribonucleotides; C487931:mirnlet7 microRNA, human