title: Four-way junction formation promoting ultrasensitive electrochemical detection of microRNA.
abstract: MicroRNAs (miRNAs) represent a class of biomarkers that are frequently deregulated in cancer cells and have shown a great promise for cancer classification and prognosis. Here, we endeavored to develop a DNA four-way junction based electrochemical sensor (4J-SENS) for ultrasensitive miRNA analysis. The developed sensor can be operated within the dynamic range from 10 aM to 1 fM and detect as low as 2 aM of miR-122 (∼36 molecules per sample), without PCR amplification. Furthermore, the 4J-SENS was employed to profile endogenouse hsa-miR-122 in healthy human and chronic lymphocyitc leukemia (CLL) patient serum, and the results were validated by qPCR analysis.
authors.affiliation: Department of Chemistry, University of Ottawa , 10 Marie Curie, Ottawa, Ontario K1N 6N5, Canada.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Labib
authors.forename: Shahrokh M
authors.initials: SM
authors.lastname: Ghobadloo
authors.forename: Nasrin
authors.initials: N
authors.lastname: Khan
authors.forename: Dmitry M
authors.initials: DM
authors.lastname: Kolpashchikov
authors.forename: Maxim V
authors.initials: MV
authors.lastname: Berezovski
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004563:Electrochemistry; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood; D057230:Limit of Detection; D035683:MicroRNAs / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D009693:Nucleic Acid Hybridization
chemical_list: C531907:MIRN122 microRNA, human; D035683:MicroRNAs; D004247:DNA