title: Functionalisation and surface modification of electrospun polylactic acid scaffold for tissue engineering.
abstract: Repair or replacement of damaged tissues using tissue engineering technology is considered to be a fine solution for enhanced treatment of different diseases such as skin diseases. Although the nanofibers made of synthetic degradable polymers, such as polylactic acid (PLA), have been widely used in the medical field, they do not favour cellular adhesion and proliferation. To enhance cell adherence on scaffold and improve biocompatibility, the surface of PLA scaffold was modified by gelatin in our experiments. For electrospinning, PLA and gelatin were dissolved in hexafluoroisopropanol (HFIP) solvent at varying compositions (PLA:gelatin at 3:7 and 7:3). The properties of the blending nanofiber scaffold were investigated by Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM). Modified PLA/gelatin 7/3 scaffold is more suitable for fibroblasts attachment and viability than the PLA or gelatin nanofiber alone. Thus fibroblast cultured on PLA/gelatin scaffold could be an alternative way to improve skin wound healing.
authors.affiliation: Department of Biology, Faculty of Biological Sciences, Kharazmi University (TMU), Tehran, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Hoveizi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Nabiuni
authors.forename: Kazem
authors.initials: K
authors.lastname: Parivar
authors.forename: Sareh
authors.initials: S
authors.lastname: Rajabi-Zeleti
authors.forename: Shima
authors.initials: S
authors.lastname: Tavakol
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002448:Cell Adhesion; D002470:Cell Survival; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D005780:Gelatin / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D057139:Nanofibers / Q000737:chemistry; D011091:Polyesters; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D020005:Propanols / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000473:pathology; D013499:Surface Properties; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D006845:Hydrocarbons, Fluorinated
chemical_list: D001672:Biocompatible Materials; D005780:Gelatin; D019344:Lactic Acid; D011091:Polyesters; D011108:Polymers; D020005:Propanols; C001337:hexafluoroisopropanol; D006845:Hydrocarbons, Fluorinated; C033616:poly(lactide)
keywords: PLA; electrospinning; gelatin; skin; tissue engineering