title: The influence of sorbitol on the production of cellulases and xylanases in an airlift bioreactor.
abstract: The production of cellulases and xylanases by Penicillium echinulatum in an airlift bioreactor was evaluated. In batch production, we tested media with isolated or associated cellulose and sorbitol. In fed-batch production, we tested cellulose addition at two different times, 30 h and 48 h. Higher liquid circulation velocities in the downcomer were observed in sorbitol 10 g L(-1) medium. In batch production, higher FPA (filter paper activity) and endoglucanase activities were obtained with cellulose (7.5 g L(-1)) and sorbitol (2.5 g L(-1)), 1.0 U mL(-1) (120 h) and 6.4 U m L(-1) (100 h), respectively. For xylanases, the best production condition was cellulose 10 g L(-1), which achieved 5.5 U mL(-1) in 64 h. The fed-batch process was favorable for obtaining xylanases, but not for FPA and endoglucanases, suggesting that in the case of cellulases, the inducer must be added early in the process.
authors.affiliation: Institute of Biotechnology, University of Caxias do Sul, Caixa Postal 1352, 95001-970 Caxias do Sul, RS, Brazil.
authors.forename: Carla Eliana Todero
authors.initials: CE
authors.lastname: Ritter
authors.forename: Roselei Claudete
authors.initials: RC
authors.lastname: Fontana
authors.forename: Marli
authors.initials: M
authors.lastname: Camassola
authors.forename: Maurício Moura
authors.initials: MM
authors.lastname: da Silveira
authors.forename: Aldo José Pinheiro
authors.initials: AJ
authors.lastname: Dillon
mesh_terms: D000388:Air; D061249:Batch Cell Culture Techniques; D019149:Bioreactors; D044602:Cellulases / Q000096:biosynthesis*; D002482:Cellulose / Q000494:pharmacology; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D010407:Penicillium / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D013012:Sorbitol / Q000494:pharmacology*
chemical_list: D013012:Sorbitol; D002482:Cellulose; D044602:Cellulases; D043364:Endo-1,4-beta Xylanases
keywords: Airlift; Batch; Cellulose; Fed-batch; Sorbitol