title: Simultaneous online enrichment and identification of trace species based on microfluidic droplets.
abstract: A facile online enrichment protocol has been proposed based on microfluidic droplets acting as an interface between a liquid chromatography separation system and detection systems of ESI-MS/MS and laser-induced fluorescence. Low-abundance species were successfully concentrated and analyzed in this system via droplet shrinkage. The proposed platform significantly increased the enrichment efficiency and detection sensitivity with reduced sample handling steps, short analysis time, and no cross-contamination. The presented system is universal, shows no discrimination, and is easily coupled with other separation and detection approaches.
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University , 220 HanDan Road, Shanghai 200433, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Ji
authors.forename: Lei
authors.initials: L
authors.lastname: Nie
authors.forename: Yixin
authors.initials: Y
authors.lastname: Li
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
authors.forename: Baohong
authors.initials: B
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D053000:Analytic Sample Preparation Methods / Q000379:methods*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D009862:Online Systems; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D012710:Serum Albumin, Bovine / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors
chemical_list: D010446:Peptide Fragments; D012710:Serum Albumin, Bovine