title: [Detection of Salmonella, Shigella and Staphylococcus aureus based on quantum dots and immunomagnetic beads].
abstract: OBJECTIVE
  To establish a detecting method of Salmonella, Shigella and Staphylococcus aureus by using quantum dots and immunomagnetic beads.
  
  
  METHODS
  The polychrome colour CdSe quantum dots were synthetized by water phase process. Triad colour quantum dots were conjugated with specific anti-Salmonella, anti-Shigella and anti-Staphylococcus aureus antibody by covalent cross-linking reaction, and then the coupled product was confirmed by SDS-PAGE and ultraviolet absorption spectrum. Using immunomagnetic enrichment and quantum dots labeling specific antibody, the fluorescence intensity of Salmonella, Shigella and Staphylococcus aureus were observed by fluorescence microscope.
  
  
  RESULTS
  The coupled reaction was successful by SDS-PAGE and ultraviolet absorption spectrum. Using immunomagnetic enrichment and quantum dots with different color and different wavelength labeling specific antibody, the detection time was less than 2h in the same reaction system. The samples infection with target bacteria were detected directly. The detection limit was 10 CFU/ml.
  
  
  CONCLUSIONS
  This method can detect Salmonella, Shigella and Staphylococcus aureus quickly and specificly.
authors.affiliation: College of Food Science and Engineering, Jilin Agriculture University, Changchun 130118, China. lqq_1986@163.com
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhang
authors.forename: Jinxing
authors.initials: J
authors.lastname: Yan
mesh_terms: D000907:Antibodies, Bacterial / Q000032:analysis; D018189:Immunomagnetic Separation / Q000379:methods*; D045663:Quantum Dots; D012475:Salmonella / Q000302:isolation & purification*; D012760:Shigella / Q000302:isolation & purification*; D013211:Staphylococcus aureus / Q000302:isolation & purification*
chemical_list: D000907:Antibodies, Bacterial