title: Cloning and characterization of a 2-Cys peroxiredoxin from Babesia gibsoni.
abstract: Peroxiredoxins (Prxs) are a family of antioxidant enzymes. Here, we cloned a 2-Cys Prx, BgTPx-1, from the canine Babesia parasite B. gibsoni. Sequence identity between BgTPx-1 and 2-Cys Prx of B. bovis was 81% at the amino acid level. Enzyme activity assay by using recombinant BgTPx-1 (rBgTPx-1) indicated that BgTPx-1 has antioxidant activity. Antiserum from a mouse immunized with rBgTPx-1 reacted with parasite lysates and detect a protein with a monomeric size of 22 kDa and also a 44 kDa protein, which might be an inefficiently reduced dimer. BgTPx-1 was expressed in the cytoplasm of B. gibsoni merozoites. These results suggest that the BgTPx-1 may play a role to control redox balance in the cytoplasm of B. gibsoni.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Tatsunori
authors.initials: T
authors.lastname: Masatani
authors.forename: Masahito
authors.initials: M
authors.lastname: Asada
authors.forename: Madoka
authors.initials: M
authors.lastname: Ichikawa-Seki
authors.forename: Miho
authors.initials: M
authors.lastname: Usui
authors.forename: Mohamad A
authors.initials: MA
authors.lastname: Terkawi
authors.forename: Kei
authors.initials: K
authors.lastname: Hayashi
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Kawazu
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001403:Babesia / Q000201:enzymology* / Q000235:genetics; D001404:Babesiosis / Q000469:parasitology / Q000662:veterinary*; D001483:Base Sequence; D015153:Blotting, Western / Q000662:veterinary; D003001:Cloning, Molecular; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D005260:Female; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D054464:Peroxiredoxins / Q000235:genetics* / Q000378:metabolism; D016053:RNA, Protozoan / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D000913:Antibodies, Protozoan; D016053:RNA, Protozoan; D011994:Recombinant Proteins; D054464:Peroxiredoxins
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