title: Dynamic molecular monitoring of retina inflammation by in vivo Raman spectroscopy coupled with multivariate analysis.
abstract: Retinal tissue is damaged during inflammation in Multiple Sclerosis. We assessed molecular changes in inflamed murine retinal cultures by Raman spectroscopy. Partial Least Squares-Discriminant analysis (PLS-DA) was able to classify retina cultures as inflamed with high accuracy. Using Multivariate Curve Resolution (MCR) analysis, we deconvolved 6 molecular components suffering dynamic changes along inflammatory process. Those include the increase of immune mediators (Lipoxygenase, iNOS and TNFα), changes in molecules involved in energy production (Cytochrome C, phenylalanine and NADH/NAD+) and decrease of Phosphatidylcholine. Raman spectroscopy combined with multivariate analysis allows monitoring the evolution of retina inflammation.
authors.affiliation: ICFO - The Institute of Photonic Sciences, Barcelona, Spain.
authors.forename: Monica
authors.initials: M
authors.lastname: Marro
authors.forename: Alice
authors.initials: A
authors.lastname: Taubes
authors.forename: Alice
authors.initials: A
authors.lastname: Abernathy
authors.forename: Stephan
authors.initials: S
authors.lastname: Balint
authors.forename: Beatriz
authors.initials: B
authors.lastname: Moreno
authors.forename: Bernardo
authors.initials: B
authors.lastname: Sanchez-Dalmau
authors.forename: Elena H
authors.initials: EH
authors.lastname: Martínez-Lapiscina
authors.forename: Ivan
authors.initials: I
authors.lastname: Amat-Roldan
authors.forename: Dmitri
authors.initials: D
authors.lastname: Petrov
authors.forename: Pablo
authors.initials: P
authors.lastname: Villoslada
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016002:Discriminant Analysis; D004734:Energy Metabolism; D007249:Inflammation / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D016018:Least-Squares Analysis; D050356:Lipid Metabolism; D051379:Mice; D057054:Molecular Imaging; D015999:Multivariate Analysis; D012164:Retinal Diseases / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D012165:Retinal Ganglion Cells / Q000473:pathology; D013059:Spectrum Analysis, Raman; D013997:Time Factors
keywords: Multiple Sclerosis; Raman spectroscopy; molecular imaging; multivariate curve resolution; neuroinflammation; retina ganglion cells