title: Solution NMR determination of hydrogen bonding and base pairing between the glyQS T box riboswitch Specifier domain and the anticodon loop of tRNA(Gly).
abstract: In Gram-positive bacteria the tRNA-dependent T box riboswitch regulates the expression of many amino acid biosynthetic and aminoacyl-tRNA synthetase genes through a transcription attenuation mechanism. The Specifier domain of the T box riboswitch contains the Specifier sequence that is complementary to the tRNA anticodon and is flanked by a highly conserved purine nucleotide that could result in a fourth base pair involving the invariant U33 of tRNA. We show that the interaction between the T box Specifier domain and tRNA consists of three Watson-Crick base pairs and that U33 confers stability to the complex through intramolecular hydrogen bonding. Enhanced packing within the Specifier domain loop E motif may stabilize the complex and contribute to cognate tRNA selection.
authors.affiliation: Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005-1892, United States.
authors.forename: Andrew T
authors.initials: AT
authors.lastname: Chang
authors.forename: Edward P
authors.initials: EP
authors.lastname: Nikonowicz
mesh_terms: D000926:Anticodon / Q000737:chemistry* / Q000378:metabolism; D020029:Base Pairing; D001483:Base Sequence; D006860:Hydrogen Bonding; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D012353:RNA, Transfer, Gly / Q000737:chemistry* / Q000378:metabolism; D058928:Riboswitch; D012996:Solutions
chemical_list: D000926:Anticodon; D012329:RNA, Bacterial; D012353:RNA, Transfer, Gly; D058928:Riboswitch; D012996:Solutions
keywords: 2D; Antitermination; CW; Difference spectrum; HMQC; Heteronuclear; NH; NOESY; Riboswitch; SD; Transcription regulation; U-turn; continuous wave; heteronuclear multiple quantum coherence; imino; nuclear Overhauser effect spectroscopy; specifier domain; tRNA; two dimensional
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