title: Characterization of Lactobacillus from Algerian Goat'S Milk Based on Phenotypic, 16S rDNA Sequencing and their Technological Properties.
abstract: Nineteen strains of Lactobacillus isolated from goat's milk from farms in north-west of Algeria were characterized. Isolates were identified by phenotypic, physiological and genotypic methods and some of their important technological properties were studied. Phenotypic characterization was carried out by studying physiological, morphological characteristics and carbohydrate fermentation patterns using API 50 CHL system. Isolates were also characterized by partial 16S rDNA sequencing. Results obtained with phenotypic methods were correlated with the genotypic characterization and 13 isolates were identified as L. plantarum, two isolates as L. rhamnosus and one isolate as L. fermentum. Three isolates identified as L. plantarum by phenotypic characterization were found to be L. pentosus by the genotypic method. A large diversity in technological properties (acid production in skim milk, exopolysaccharide production, aminopeptidase activity, antibacterial activity and antibiotic susceptibility) was observed. Based on these results, two strains of L. plantarum (LbMS16 and LbMS21) and one strain of L. rhamnosus (LbMF25) have been tentatively selected for use as starter cultures in the manufacture of artisanal fermented dairy products in Algeria.
authors.affiliation: Laboratory of Applied Microbiology, Department of Biotechnology (IGMO), University of Oran Es-Sénia , Oran, 31100 , Algeria.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Marroki
authors.forename: Manuel
authors.initials: M
authors.lastname: Zúñiga
authors.forename: Mabrouk
authors.initials: M
authors.lastname: Kihal
authors.forename: Gaspar
authors.initials: G
authors.lastname: Pérez-Martínez
keywords: Algeria; Lactic acid bacteria; Lactobacillus; goat’s milk; identification; technological properties
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