title: Identification, antimicrobial resistance and genotypic characterization of Enterococcus spp. isolated in Porto Alegre, Brazil.
abstract: In the past two decades the members of the genus Enterococcus have emerged as important nosocomial pathogens worldwide. In the present study, we evaluated the antimicrobial resistance and genotypic characteristics of 203 Enterococcus spp. recovered from different clinical sources from two hospitals in Porto Alegre, Rio Grande do Sul, Brazil. The species were identified by conventional biochemical tests and by an automated system. The genetic diversity of E. faecalis presenting high-level aminoglycoside resistance (HLAR) was assessed by pulsed-field gel electrophoresis of chromosomal DNA after SmaI digestion. The E. faecalis was the most frequent specie (93.6%), followed by E. faecium (4.4%). The antimicrobial resistance profile was: 2.5% to ampicillin, 0.5% to vancomycin, 0.5% teicoplanin, 33% to chloramphenicol, 2% to nitrofurantoin, 66.1% to erythromycin, 66.5% to tetracycline, 24.6% to rifampicin, 30% to ciprofloxacin and 87.2% to quinupristin-dalfopristin. A total of 10.3% of the isolates proved to be HLAR to both gentamicin and streptomycin (HLR-ST/GE), with 23.6% resistant only to gentamicin (HLR-GE) and 37.4% only to streptomycin (HLR-ST). One predominant clonal group was found among E. faecalis HLR-GE/ST. The prevalence of resistance among beta-lactam antibiotics and glycopeptides was very low. However, in this study there was an increased number of HLR Enterococcus which may be spreading intra and inter-hospital.
authors.affiliation: Programa de Pós- Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul , Porto Alegre, RS , Brasil.
authors.forename: Eduardo André
authors.initials: EA
authors.lastname: Bender
authors.forename: Ana Lúcia Peixoto
authors.initials: AL
authors.lastname: de Freitas
authors.forename: Keli Cristine
authors.initials: KC
authors.lastname: Reiter
authors.forename: Larissa
authors.initials: L
authors.lastname: Lutz
authors.forename: Afonso Luís
authors.initials: AL
authors.lastname: Barth
keywords: Enterococcus; HLAR; PFGE; resistance
references.citation: J Clin Microbiol. 1989 Apr;27(4):731-4
references.pmid: 2656745
references.citation: Braz J Med Biol Res. 2000 Nov;33(11):1269-74
references.pmid: 11050655
references.citation: Clin Infect Dis. 1997 Jan;24(1):90-1
references.pmid: 8994759
references.citation: Clin Microbiol Rev. 2000 Oct;13(4):686-707
references.pmid: 11023964
references.citation: J Clin Microbiol. 1995 Sep;33(9):2233-9
references.pmid: 7494007
references.citation: Microbes Infect. 2002 Feb;4(2):215-24
references.pmid: 11880055
references.citation: Diagn Microbiol Infect Dis. 2007 Jun;58(2):163-70
references.pmid: 17368801
references.citation: J Med Microbiol. 2004 Dec;53(Pt 12):1229-1232
references.pmid: 15585502
references.citation: Rev Inst Med Trop Sao Paulo. 2006 Jan-Feb;48(1):11-6
references.pmid: 16547573
references.citation: N Engl J Med. 2000 Mar 9;342(10):710-21
references.pmid: 10706902
references.citation: Microb Drug Resist. 2003 Summer;9(2):167-74
references.pmid: 12820802
references.citation: J Antimicrob Chemother. 2005 Nov;56(5):827-35
references.pmid: 16186168
references.citation: Int J Antimicrob Agents. 2005 Jun;25(6):535-8
references.pmid: 15908184
references.citation: Diagn Microbiol Infect Dis. 2005 Jan;51(1):63-7
references.pmid: 15629231
references.citation: Rev Saude Publica. 2005 Feb;39(1):41-6
references.pmid: 15654459