title: Increased IL10 plasmatic levels in Fanconi anemia patients.
abstract: Fanconi anemia (FA) is a rare disease, autosomal recessive and X linked, which is clinically prone to development of hematological abnormalities and neoplasms, especially acute myeloid leukemia. In this work IL-10 and TGF-β levels were measured on FA patients' plasma since they are the regulatory cytokines of TNF-α and INF-γ which had been described to be overexpressed in this genetic disease. Our results show increased IL-10 plasma levels in 25% of FA patients studied, but levels of TGF-β within the normal range. TNF-α and INF-γ were also measured and found to be increased in 24% and 23% of FA patients, respectively. However, no inverse correlation was observed between augmented levels of IL-10 and TNF or IFN-γ. Patients with elevated levels of TNF-α and INF-γ presented bone marrow hypocellularity. IL-10 levels did not appear to be determinant for bone marrow cellularity. These data suggest that IL-10 is also a feature of Fanconi anemia pathophysiology.
authors.affiliation: Departamento de Bioquímica/IBRAG, UERJ, Brazil; Instituto de Bioquímica Médica/CCS, UFRJ, Brazil. Electronic address: magrajusto@hotmail.com.
authors.forename: Graça A
authors.initials: GA
authors.lastname: Justo
authors.forename: Marco A
authors.initials: MA
authors.lastname: Bitencourt
authors.forename: Ricardo
authors.initials: R
authors.lastname: Pasquini
authors.forename: Morgana T L
authors.initials: MT
authors.lastname: Castelo-Branco
authors.forename: Vivian M
authors.initials: VM
authors.lastname: Rumjanek
mesh_terms: D000293:Adolescent; D000328:Adult; D001792:Blood Platelets / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005199:Fanconi Anemia / Q000097:blood*; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000097:blood*; D008297:Male; D055815:Young Adult
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10
keywords: Cytokines; ELISA’ assay; Fanconi anemia; Immune system