title: Mouse strain does not influence the overall effects of bisphenol a-induced toxicity in adult antral follicles.
abstract: Bisphenol A (BPA) is an endocrine-disrupting chemical (EDC) widely used in common consumer products containing polycarbonate plastics and epoxy resins. Previous studies indicate that other EDCs have species-dependent effects. Furthermore, some EDCs are known to have different effects in different strains within the same species. Little information, however, is known about whether the effects of BPA on the ovary differ by strain. Previous studies have shown that BPA inhibits follicle growth, induces atresia, and inhibits steroidogenesis and expression of steroidogenic enzymes in antral follicles from adult FVB mice. Thus, this study was designed to expand previous work by testing the hypothesis that mouse strain may differentially affect the susceptibility of adult antral follicles to BPA-induced toxicity. To test this hypothesis, antral follicles were mechanically isolated from adult FVB, CD-1, and C57BL/6 mice, individually cultured for 6-120 h and treated with either vehicle control (dimethylsulfoxide) or various concentrations of BPA (1.0 μg/ml, 10 μg/ml, or 100 μg/ml). After culture, media were subjected to measurements of hormone production via ELISA, and follicles were subjected to real-time PCR for analysis of genes known to regulate steroidogenesis, the cell cycle, and atresia. Overall, BPA inhibited follicle growth and steroidogenesis in all tested strains, but CD-1 follicles were slightly more sensitive to BPA at early time points than FVB and C57BL/6 follicles. These data suggest that CD-1, FVB, and C57BL/6 mice can all be used to investigate the effects of BPA on ovarian follicles.
authors.affiliation: Department of Comparative Biosciences, University of Illinois at Urbana-Champaign, Urbana, Illinois.
authors.forename: Jackye
authors.initials: J
authors.lastname: Peretz
authors.forename: Steven L
authors.initials: SL
authors.lastname: Neese
authors.forename: Jodi A
authors.initials: JA
authors.lastname: Flaws
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D002453:Cell Cycle / Q000187:drug effects; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics / Q000378:metabolism; D052244:Endocrine Disruptors / Q000633:toxicity*; D005260:Female; D005496:Follicular Atresia / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D006080:Ovarian Follicle / Q000187:drug effects* / Q000502:physiology; D010636:Phenols / Q000633:toxicity*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D000098682:Steroidogenic Acute Regulatory Protein; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D002786:Cholesterol Side-Chain Cleavage Enzyme; D052244:Endocrine Disruptors; D010636:Phenols; D010750:Phosphoproteins; D000098682:Steroidogenic Acute Regulatory Protein; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: bisphenol A; follicle growth; steroidogenesis; strain
references.citation: Endocrinology. 2011 Mar;152(3):1101-11
references.pmid: 21285323
references.citation: Reprod Toxicol. 2010 Nov;30(3):393-400
references.pmid: 20599497
references.citation: Toxicol Sci. 2005 Nov;88(1):213-21
references.pmid: 16081520
references.citation: Environ Health Perspect. 2007 Apr;115(4):592-8
references.pmid: 17450229
references.citation: Toxicol Sci. 2011 Jan;119(1):209-17
references.pmid: 20956811
references.citation: Reprod Toxicol. 2011 Feb;31(2):177-83
references.pmid: 21055461
references.citation: Environ Health Perspect. 2005 Apr;113(4):391-5
references.pmid: 15811827
references.citation: J Vet Med Sci. 2011 Sep;73(9):1211-5
references.pmid: 21532259
references.citation: Toxicol Sci. 2006 Oct;93(2):382-9
references.pmid: 16807286
references.citation: Biol Reprod. 1999 Oct;61(4):851-6
references.pmid: 10491616
references.citation: Environ Health Perspect. 2002 Apr;110(4):427-31
references.pmid: 11940462
references.citation: Reprod Toxicol. 2011 Nov;32(3):304-12
references.pmid: 21722727
references.citation: Toxicol Appl Pharmacol. 2013 Sep 1;271(2):249-56
references.pmid: 23707772
references.citation: Reprod Toxicol. 2008 Oct;26(2):94-9
references.pmid: 18638542
references.citation: Life Sci. 2009 Nov 18;85(21-22):742-52
references.pmid: 19837096
references.citation: Environ Health Perspect. 2010 Sep;118(9):1217-22
references.pmid: 20413367
references.citation: Biol Reprod. 2012 Sep 21;87(3):63
references.pmid: 22743301
references.citation: ILAR J. 2004;45(4):387-93
references.pmid: 15454677
references.citation: Science. 1999 Aug 20;285(5431):1259-61
references.pmid: 10455051
references.citation: Toxicol Sci. 2008 Aug;104(2):362-84
references.pmid: 18445619
references.citation: ILAR J. 2006;47(2):94-102
references.pmid: 16547366
references.citation: Environ Health Perspect. 2005 Aug;113(8):926-33
references.pmid: 16079060
references.citation: Environ Health Perspect. 2000 Mar;108(3):243-7
references.pmid: 10706531
references.citation: Anim Reprod Sci. 2007 Jun;99(3-4):413-20
references.pmid: 16889909
references.citation: Mol Biol Rep. 2012 Sep;39(9):8621-8
references.pmid: 22699882
references.citation: Environ Sci Pollut Res Int. 2011 Aug;19(7):2515-27
references.pmid: 22828881
references.citation: J Toxicol Sci. 2012;37(3):565-73
references.pmid: 22687996
references.citation: Birth Defects Res B Dev Reprod Toxicol. 2011 Dec;92(6):526-33
references.pmid: 21922642
references.citation: Clin Exp Reprod Med. 2011 Jun;38(2):75-81
references.pmid: 22384422
references.citation: Reprod Toxicol. 2011 Dec;32(4):434-41
references.pmid: 21907787
references.citation: Toxicology. 2009 Nov 9;265(1-2):56-67
references.pmid: 19782717
references.citation: J Reprod Fertil. 1995 Mar;103(2):293-8
references.pmid: 7616502
references.citation: Biol Reprod. 2011 Jan;84(1):79-86
references.pmid: 20739668
references.citation: Crit Rev Toxicol. 2006 May;36(5):387-457
references.pmid: 16954066
references.citation: Reproduction. 2010 Feb;139(2):349-57
references.pmid: 19846484
references.citation: APMIS. 2001 May;109(5):356-64
references.pmid: 11478683
references.citation: Reprod Toxicol. 2011 May;31(4):409-17
references.pmid: 21182934