title: Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy.
abstract: The PreS1 region of the L protein is important in cell attachment and consequently in hepatitis B virus (HBV) infectivity. To identify novel PreS1 interacting protein, we performed Glutathione-S-transferase (GST) pull-down, two-dimensional gel electrophoresis (2-DE) and mass spectrometry assays. Glucose-regulated proteins (GRP) 78 and 75 were found to bind PreS1. The interactions between PreS1 and GRP75 were confirmed by a co-immunoprecipitation experiment. GRP78 and GRP75 may play important roles in mediating HBV virion entering into hepatocyte and regulating proper folding of the L protein due to their critical functions in protein folding and trafficking. The finding of novel PreS1 binding protein enriches our knowledge about molecular mechanism of HBV infection.
authors.affiliation: Institute of Microbiology, Jiangsu Provincial Center for Diseases Prevention and Control , Nanjing 210009 , China.
authors.forename: Lunbiao
authors.initials: L
authors.lastname: Cui
authors.forename: Yiyue
authors.initials: Y
authors.lastname: Ge
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Qi
authors.forename: Zhiyang
authors.initials: Z
authors.lastname: Shi
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Jiao
authors.forename: Xian
authors.initials: X
authors.lastname: Qi
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Zhai
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
keywords: GRP75; HBV; PreS1; Proteomics
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