title: Specific Link between Lung and Large Intestine: A New Perspective on Neuropeptide Secretion in Lung with Herbal Laxative Stimulation.
abstract: Background. To investigate the specific link between lung and large intestine. Methods. Rat COPD-like model was prepared. Mirabilite or Chinese rhubarb was administrated intragastrically to stimulate the large intestine. Histological analysis of lung inflammation was assessed. The tissues levels of SP, VIP, NK1R, VIPR1, and VIPR2 were measured by using ELISA kits. In addition, mouse model of allergic asthma was prepared. Mirabilite was administrated intragastrically to stimulate the large intestine. Airway responsiveness and lung inflammation were assessed. The tissues levels of SP, VIP, NKA, NKB, NK1R, VIPR1, and VIPR2 were measured by using ELISA kits. Results. Stimulating the intestine with Mangxiao or Dahuang, SP, NK-1R, VIP, VIPR1, and VIPR2 were significantly increased in intestine tissues of rats with COPD and mice with asthma. Meanwhile, the SP and NK1R were significantly decreased, while VIP, VIPR1, and VIPR2 were significantly increased in lung tissues. An abnormal secretion of SP and VIP can be observed in other tissues; however, no marked changes were found in the receptors. The NKA and NKB levels were similar in lung tissues of mice with asthma among groups. Conclusions. Stimulating intestine with Mangxiao or Dahuang can specifically regulate the secretion of SP, VIP, and the receptors in lung tissues.
authors.affiliation: School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Xiang-Gen
authors.initials: XG
authors.lastname: Zhong
authors.forename: Feng-Jie
authors.initials: FJ
authors.lastname: Zheng
authors.forename: Yu-Hang
authors.initials: YH
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Yu-Chao
authors.initials: YC
authors.lastname: Liu
authors.forename: Miao
authors.initials: M
authors.lastname: Liu
authors.forename: Ruo-Han
authors.initials: RH
authors.lastname: Wu
authors.forename: Yu-Shan
authors.initials: YS
authors.lastname: Gao
authors.forename: Shu-Jing
authors.initials: SJ
authors.lastname: Zhang
authors.forename: Jin-Chao
authors.initials: JC
authors.lastname: Zhang
authors.forename: Tian-Yu
authors.initials: TY
authors.lastname: Zhang
authors.forename: Si-Hua
authors.initials: SH
authors.lastname: Gao
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