title: A toolbox for tuberculosis (TB) diagnosis: an Indian multicentric study (2006-2008). Evaluation of QuantiFERON-TB gold in tube for TB diagnosis.
abstract: BACKGROUND
  The aim of this multicentric prospective study in India was to assess the performance of the QuantiFERON TB-Gold in tube (QFT-GIT), Tuberculin Skin Test (TST) and microbiological results as additional tools for diagnosing active tuberculosis (TB) and latent infection (LTBI) according to Human Immunodeficiency Virus (HIV) status.
  
  
  METHODS
  Individuals with and without active TB and HIV infection were enrolled between 2006-2008. QFT-GIT and TST results were analyzed per se and in combination with microbiological data.
  
  
  RESULTS
  Among the 276 individuals (96 active pulmonary TB and 180 no active TB) tested by QFT-GIT, 18 indeterminate results (6.5%) were found, more significantly numerous in the HIV-infected (15/92; 16.3%) than the HIV-uninfected (3/184; 1.6%)(p<0.0001). QFT-GIT sensitivity for active TB was 82.3% and 92.9% respectively after including or excluding indeterminate results. Clinical sensitivity was significantly lower in the HIV-infected (68.4%) than the HIV-uninfected (91.4%) patients (p = 0.0059). LTBI was detected in 49.3% of subjects without active TB but varied according to TB exposure. When the TST and QFT-GIT were concomitantly performed, the respective sensitivity for active TB diagnosis was 95.0% and 85.0% in the HIV-uninfected (p = 0.60), and 66.7% and 51.5% in the HIV-infected patients (p = 0.32). QFT-GIT and TST respective specificity for active TB in the HIV-uninfected was 25.0% and 57.1% (p = 0.028), and 64.8% and 83.3% in the HIV-infected (p = 0.047). In those with active TB, QFT-GIT results were not associated with microbiological parameters (smear grade, liquid culture status, time-to-positivity of culture) or clinical suspicion of active TB score (provided by the clinicians at enrollment). Combining microbiological tests with both immunological tests significantly increased sensitivity for active TB diagnosis (p = 0.0002), especially in the HIV-infected individuals (p = 0.0016).
  
  
  CONCLUSIONS
  QFT-GIT and TST have similar diagnostic value for active TB diagnosis. In HIV-infected patients, combining microbiological tests with both immunological tests significantly increases the sensitivity for active TB diagnosis.
authors.affiliation: Service de Microbiologie, Hôpital Saint Louis, Assistance Publique- Hôpitaux de Paris, Paris VII Denis Diderot University, Paris, France.
authors.forename: Philippe H
authors.initials: PH
authors.lastname: Lagrange
authors.forename: Satheesh K
authors.initials: SK
authors.lastname: Thangaraj
authors.forename: Rajeshwar
authors.initials: R
authors.lastname: Dayal
authors.forename: Alaka
authors.initials: A
authors.lastname: Deshpande
authors.forename: Nirmal K
authors.initials: NK
authors.lastname: Ganguly
authors.forename: Enrico
authors.initials: E
authors.lastname: Girardi
authors.forename: Beenu
authors.initials: B
authors.lastname: Joshi
authors.forename: Kiran
authors.initials: K
authors.lastname: Katoch
authors.forename: Vishwa M
authors.initials: VM
authors.lastname: Katoch
authors.forename: Manoj
authors.initials: M
authors.lastname: Kumar
authors.forename: Vemu
authors.initials: V
authors.lastname: Lakshmi
authors.forename: Marc
authors.initials: M
authors.lastname: Leportier
authors.forename: Christophe
authors.initials: C
authors.lastname: Longuet
authors.forename: Subbalaxmi V S
authors.initials: SV
authors.lastname: Malladi
authors.forename: Deepali
authors.initials: D
authors.lastname: Mukerjee
authors.forename: Deepthi
authors.initials: D
authors.lastname: Nair
authors.forename: Alamelu
authors.initials: A
authors.lastname: Raja
authors.forename: Balambal
authors.initials: B
authors.lastname: Raman
authors.forename: Camilla
authors.initials: C
authors.lastname: Rodrigues
authors.forename: Pratibha
authors.initials: P
authors.lastname: Sharma
authors.forename: Amit
authors.initials: A
authors.lastname: Singh
authors.forename: Sarman
authors.initials: S
authors.lastname: Singh
authors.forename: Archana
authors.initials: A
authors.lastname: Sodha
authors.forename: Basirudeen Syed Ahamed
authors.initials: BS
authors.lastname: Kabeer
authors.forename: Guy
authors.initials: G
authors.lastname: Vernet
authors.forename: Delia
authors.initials: D
authors.lastname: Goletti
mesh_terms: D000328:Adult; D005260:Female; D015658:HIV Infections / Q000150:complications / Q000175:diagnosis; D006801:Humans; D059425:Interferon-gamma Release Tests / Q000379:methods; D055985:Latent Tuberculosis / Q000150:complications / Q000175:diagnosis; D008297:Male; D008828:Microbiological Techniques / Q000379:methods; D008875:Middle Aged; D009161:Mycobacterium / Q000302:isolation & purification*; D014374:Tuberculin Test / Q000379:methods; D014376:Tuberculosis / Q000150:complications / Q000175:diagnosis*; D055815:Young Adult
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