title: Frequency of diarrheagenic Escherichia coli among children in Surabaya, Indonesia.
abstract: Diarrheagenic Escherichia coli (DEC) is a major etiologic agent of childhood diarrhea in developing countries. We investigated the frequency of DEC in stool samples from 125 diarrheal children (age, 1-10 years) and 92 non-diarrheal children in Surabaya, Indonesia. The non-diarrheal children served as healthy controls. DEC was detected in 23 of 125 (18.4%) and 47 of 92 (51.1%) samples in the diarrheal and non-diarrheal children, respectively. Enteropathogenic E. coli was the most prevalent in the non-diarrheal children (25.0%), and its prevalence was significantly higher than that in the diarrheal children (0.8%) (P < 0.0001). Interestingly, Shiga toxin-producing E. coli (4.3%) was detected only in the non-diarrheal children (P = 0.031). This is the first study comparing between diarrheal children with non-diarrheal or healthy children to investigate the role of DEC in pediatric diarrheal diseases in Indonesia.
authors.affiliation: Division of Infectious Diseases, Department of International Health, Kobe University Graduate School of Health Sciences.
authors.forename: Kayo
authors.initials: K
authors.lastname: Osawa
authors.forename: Dadik
authors.initials: D
authors.lastname: Raharjo
authors.forename: Eddy Bagus
authors.initials: EB
authors.lastname: Wasito
authors.forename: Sugeng
authors.initials: S
authors.lastname: Harijono
authors.forename: Katsumi
authors.initials: K
authors.lastname: Shigemura
authors.forename: Ro
authors.initials: R
authors.lastname: Osawa
authors.forename: Subijanto Marto
authors.initials: SM
authors.lastname: Sudarmo
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Iijima
authors.forename: Toshiro
authors.initials: T
authors.lastname: Shirakawa
mesh_terms: D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000235:genetics; D003967:Diarrhea / Q000453:epidemiology* / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics; D005243:Feces / Q000382:microbiology; D005260:Female; D005838:Genotype; D006801:Humans; D007214:Indonesia / Q000453:epidemiology; D007223:Infant; D008297:Male; D015995:Prevalence; D037521:Virulence Factors / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D037521:Virulence Factors