title: SIRT4 prevents hypoxia-induced apoptosis in H9c2 cardiomyoblast cells.
abstract: OBJECTIVE
  Apoptosis plays a critical role in cardiomyocyte loss during ischaemic heart injury. A detailed understanding of the mechanism involved has a substantial impact on the optimization and development of treatment strategies. Here, we report that the expression of SIRT4, a mitochondrial sirtuin, is markedly down-regulated in hypoxia-induced apoptosis of H9c2 cardiomyoblast cells.
  
  
  RESULTS
  SIRT4 interference significantly alters H9c2 cell viability, apoptotic cell number and caspase-3/7 activity. Furthermore, SIRT4 expression can affect the ratio of pro-caspase 9/caspase 9 or pro-caspase 3/caspase 3, an affect Bax translocation, which in turn alters the development of H9c2 cell apoptosis.
  
  
  CONCLUSIONS
  These results suggest that SIRT4 is a key player in hypoxia-induced cardiomyocyte apoptosis, and that strategies based on its enhancement might be of benefit in the treatment of ischaemic heart disease.
authors.affiliation: Department of Cardiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. China.
authors.forename: Ban
authors.initials: B
authors.lastname: Liu
authors.forename: Wenliang
authors.initials: W
authors.lastname: Che
authors.forename: Jinsong
authors.initials: J
authors.lastname: Xue
authors.forename: Changzhu
authors.initials: C
authors.lastname: Zheng
authors.forename: Kai
authors.initials: K
authors.lastname: Tang
authors.forename: Jingying
authors.initials: J
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Wen
authors.forename: Yawei
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D015687:Cell Hypoxia; D002460:Cell Line; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D051381:Rats; D037761:Sirtuins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D011498:Protein Precursors; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051028:bcl-2-Associated X Protein; D053148:Caspase 3; D053179:Caspase 7; D053453:Caspase 9; C530376:SIRT5 protein, rat; D037761:Sirtuins