title: Rapid high-specificity microRNA detection using a two-stage isotachophoresis assay.
abstract: Focusing in on the small: A two-stage microRNA detection assay uses electrokinetic focusing to speed up hybridization and a functionalized hydrogel for affinity purification. This method detects microRNAs in 15 minutes with single-nucleotide specificity, and processes only 5 ng of total RNA.
authors.affiliation: Department of Mechanical Engineering, Stanford University, 440 Escondido Mall, Building 530, Room 225, Stanford, CA 94305 (USA).
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Garcia-Schwarz
authors.forename: Juan G
authors.initials: JG
authors.lastname: Santiago
mesh_terms: D006801:Humans; D057625:Isotachophoresis / Q000379:methods*; D035683:MicroRNAs / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000379:methods*
chemical_list: D035683:MicroRNAs
keywords: RNA recognition; RNA structures; analytical methods; hybridization assay; isotachophoresis