title: Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine.
abstract: The aim of this study was to compare the PSPT standardized in-house as an alternative to MPT for potency assays of pertussis component. Statistical analyses have showed similar pertussis potency values when PSPT was compared to MPT. Significant correlation between the potency results obtained by in vivo and in vitro assays was also been observed. Results by PSPT have demonstrated reproducibility and accuracy for potency pertussis control and this approach has been considered promising for use at least during the steps of production.
authors.affiliation: Laboratório de Tecnologia Imunológica, Bio-Manguinhos, Fundação Instituto Oswaldo Cruz , Rio de Janeiro, RJ , Brasil.
authors.forename: Denise Cristina Souza
authors.initials: DC
authors.lastname: Matos
authors.forename: Rugimar
authors.initials: R
authors.lastname: Marcovistz
authors.forename: Andréa Marques Vieira
authors.initials: AM
authors.lastname: da Silva
authors.forename: Wagner
authors.initials: W
authors.lastname: Quintilio
authors.forename: Ricardo Amaral
authors.initials: RA
authors.lastname: Georgini
keywords: DTP vaccine; Pertussis whole cell; enzyme-linked immunosorbent assay; intracerebral mouse protection test
references.citation: Mem Inst Oswaldo Cruz. 2002 Sep;97(6):909-13
references.pmid: 12386721
references.citation: Biologicals. 1994 Sep;22(3):233-42
references.pmid: 7811457
references.citation: Biologicals. 2002 Jun;30(2):105-12
references.pmid: 12127312
references.citation: Biologicals. 1994 Sep;22(3):257-68
references.pmid: 7811460
references.citation: Vaccine. 2000 Dec 8;19(9-10):1118-28
references.pmid: 11137247
references.citation: Altern Lab Anim. 1995 May-Jun;23(3):298-304
references.pmid: 11656565
references.citation: Dev Biol Stand. 1996;86:3-10
references.pmid: 8785959