title: Cd-doped ZnO quantum dots-based immunoassay for the quantitative determination of bisphenol A.
abstract: Bisphenol A (BPA) is a ubiquitous environmental contaminant in food products and aquatic ecosystems. Its endocrine and developmental toxicity presents a serious concern to human health and an effective high-throughput method for its detection is desirable. In this paper, water-soluble quantum dots (QDs) have been conjugated covalently with BPA antibodies and the conjugate has been utilized in a competitive fluorescence-linked immunoassay (FLISA). Cd-doped ZnO QDs were functionalized with poly(amidoamine) (PAMAM) dendrimers, as evidenced by ultraviolet absorption spectrum and fluorescence emission spectra analyses, and this led to their successful transfer into aqueous solution. Biological mass spectrometry demonstrated that the bisphenol A antibodies were successfully coupled to the water-soluble QDs, and the structures of these conjugates kept intact. The FLISA method allowed for BPA determination in a linear working range of 20.8-330.3 ng mL(-1) with the limit of detection (LOD) of 13.1 ng mL(-1). The recoveries of BPA from water samples were from 85.92% to 109.62%. In conclusion, a rapid and sensitive FLISA was developed by utilizing novel QD coupling method and validated for use in aqueous samples.
authors.affiliation: Department of Pharmaceutical Engineering, Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Su-Qing
authors.initials: SQ
authors.lastname: Zhao
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.forename: Jian-Qing
authors.initials: JQ
authors.lastname: Zhou
mesh_terms: D000906:Antibodies / Q000737:chemistry; D001559:Benzhydryl Compounds / Q000032:analysis* / Q000737:chemistry; D002104:Cadmium / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry; D015034:Zinc Oxide / Q000737:chemistry*; D000098826:Bisphenol A Compounds
chemical_list: D000906:Antibodies; D001559:Benzhydryl Compounds; D002104:Cadmium; D010636:Phenols; D014874:Water Pollutants, Chemical; D015034:Zinc Oxide; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A (BPA); Competitive fluorescence-linked immunosorbent assay (FLISA); Immunoassay method; Quantum dots (QDs)