title: Vatairea macrocarpa lectin (VML) induces depressive-like behavior and expression of neuroinflammatory markers in mice.
abstract: Lectins are proteins capable of reversible binding to the carbohydrates in glycoconjugates that can regulate many physiological and pathological events. Galectin-1, a β-galactoside-binding lectin, is expressed in the central nervous system (CNS) and exhibits neuroprotective functions. Additionally, lectins isolated from plants have demonstrated beneficial action in the CNS. One example is a lectin with mannose-glucose affinity purified from Canavalia brasiliensis seeds, ConBr, which displays neuroprotective and antidepressant activity. On the other hand, the effects of the galactose-binding lectin isolated from Vatairea macrocarpa seeds (VML) on the CNS are largely unknown. The aim of this study was to verify if VML is able to alter neural function by evaluating signaling enzymes, glial and inflammatory proteins in adult mice hippocampus, as well as behavioral parameters. VML administered by intracerebroventricular (i.c.v) route increased the immobility time in the forced swimming test (FST) 60 min after its injection through a carbohydrate recognition domain-dependent mechanism. Furthermore, under the same conditions, VML caused an enhancement of COX-2, GFAP and S100B levels in mouse hippocampus. However, phosphorylation of Akt, GSK-3β and mitogen-activated protein kinases named ERK1/2, JNK1/2/3 and p38(MAPK), was not changed by VML. The results reported here suggest that VML may trigger neuroinflammatory response in mouse hippocampus and exhibit a depressive-like activity. Taken together, our findings indicate a dual role for galactose binding lectins in the modulation of CNS function.
authors.affiliation: Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, 88040-900, Brazil.
authors.forename: Filipe Marques
authors.initials: FM
authors.lastname: Gonçalves
authors.forename: Andiara Espíndola
authors.initials: AE
authors.lastname: Freitas
authors.forename: Tanara Vieira
authors.initials: TV
authors.lastname: Peres
authors.forename: Débora Kurrle
authors.initials: DK
authors.lastname: Rieger
authors.forename: Juliana
authors.initials: J
authors.lastname: Ben
authors.forename: Mariana
authors.initials: M
authors.lastname: Maestri
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Costa
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Tramontina
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Gonçalves
authors.forename: Ana Lúcia Severo
authors.initials: AL
authors.lastname: Rodrigues
authors.forename: Celso Shiniti
authors.initials: CS
authors.lastname: Nagano
authors.forename: Edson Holanda
authors.initials: EH
authors.lastname: Teixeira
authors.forename: Kyria S
authors.initials: KS
authors.lastname: Nascimento
authors.forename: Benildo Sousa
authors.initials: BS
authors.lastname: Cavada
authors.forename: Rodrigo Bainy
authors.initials: RB
authors.lastname: Leal
mesh_terms: D000818:Animals; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D003863:Depression / Q000139:chemically induced*; D007887:Fabaceae / Q000737:chemistry*; D005690:Galactose / Q000494:pharmacology; D005904:Glial Fibrillary Acidic Protein; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D007276:Injections, Intraventricular; D037102:Lectins / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D064568:S100 Calcium Binding Protein beta Subunit / Q000096:biosynthesis; D013550:Swimming
chemical_list: D005904:Glial Fibrillary Acidic Protein; D037102:Lectins; D009419:Nerve Tissue Proteins; D064568:S100 Calcium Binding Protein beta Subunit; C579159:S100b protein, mouse; C528900:glial fibrillary astrocytic protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D005690:Galactose
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