title: A novel system for spatial and temporal imaging of intrinsic plant water use efficiency.
abstract: Instrumentation and methods for rapid screening and selection of plants with improved water use efficiency are essential to address current issues of global food and fuel security. A new imaging system that combines chlorophyll fluorescence and thermal imaging has been developed to generate images of assimilation rate (A), stomatal conductance (gs), and intrinsic water use efficiency (WUEi) from whole plants or leaves under controlled environmental conditions. This is the first demonstration of the production of images of WUEi and the first to determine images of g s from themography at the whole-plant scale. Data are presented illustrating the use of this system for rapidly and non-destructively screening plants for alterations in WUEi by comparing Arabidopsis thaliana mutants (OST1-1) that have altered WUEi driven by open stomata, with wild-type plants. This novel instrument not only provides the potential to monitor multiple plants simultaneously, but enables intra- and interspecies variation to be taken into account both spatially and temporally. The ability to measure A, gs, and WUEi progressively was developed to facilitate and encourage the development of new dynamic protocols. Images illustrating the instrument's dynamic capabilities are demonstrated by analysing plant responses to changing photosynthetic photon flux density (PPFD). Applications of this system will augment the research community's need for novel screening methods to identify rapidly novel lines, cultivars, or species with improved A and WUEi in order to meet the current demands on modern agriculture and food production.
authors.affiliation: School of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, UK.
authors.forename: L
authors.initials: L
authors.lastname: McAusland
authors.forename: P A
authors.initials: PA
authors.lastname: Davey
authors.forename: N
authors.initials: N
authors.lastname: Kanwal
authors.forename: N R
authors.initials: NR
authors.lastname: Baker
authors.forename: T
authors.initials: T
authors.lastname: Lawson
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D061848:Optical Imaging; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D014867:Water / Q000378:metabolism*
chemical_list: D014867:Water; D002245:Carbon Dioxide
keywords: Chlorophyll fluorescence imaging; dynamic responses; leaf heterogeneity; screening; thermal imaging; water use efficiency.
references.citation: Plant J. 2002 Jun;30(5):601-9
references.pmid: 12047634
references.citation: Plant Methods. 2008 May 27;4:12
references.pmid: 18505561
references.citation: Oecologia. 2012 Aug;169(4):905-13
references.pmid: 22358995
references.citation: Philos Trans R Soc Lond B Biol Sci. 2008 Feb 12;363(1491):639-58
references.pmid: 17652070
references.citation: J Exp Bot. 2006;57(3):527-36
references.pmid: 16377737
references.citation: Biotechnol J. 2009 Aug;4(8):1152-67
references.pmid: 19557794
references.citation: Photosynth Res. 1992 Mar;31(3):195-212
references.pmid: 24408060
references.citation: J Exp Bot. 2004 May;55(400):1187-93
references.pmid: 15073209
references.citation: Funct Plant Biol. 2009 Nov;36(11):867-873
references.pmid: 32688697
references.citation: Tree Physiol. 2012 Jul;32(7):839-46
references.pmid: 22696269
references.citation: Plant Physiol. 2003 Jun;132(2):485-93
references.pmid: 12805581
references.citation: Plant Cell Environ. 2013 Oct;36(10):1755-70
references.pmid: 23586649
references.citation: J Exp Bot. 2002 Apr;53(369):737-46
references.pmid: 11886894
references.citation: J Exp Bot. 2004 Nov;55(407):2447-60
references.pmid: 15475373
references.citation: Trends Biotechnol. 2005 Jun;23(6):308-15
references.pmid: 15922083
references.citation: Plant Physiol. 1997 Nov;115(3):1163-1174
references.pmid: 12223865
references.citation: J Exp Bot. 2013 Oct;64(13):3937-49
references.pmid: 23599272
references.citation: New Phytol. 2009;181(1):13-34
references.pmid: 19076715
references.citation: J Exp Bot. 2007;58(4):773-84
references.pmid: 17189594
references.citation: Curr Opin Biotechnol. 2012 Apr;23(2):215-20
references.pmid: 22296828
references.citation: Plant Physiol. 2006 Feb;140(2):771-8
references.pmid: 16407445
references.citation: Plant Physiol. 1990 Jul;93(3):1078-82
references.pmid: 16667560
references.citation: Photosynth Res. 2006 Nov;90(2):161-72
references.pmid: 17211583
references.citation: Nature. 2012 Oct 11;490(7419):254-7
references.pmid: 22932270
references.citation: J Exp Bot. 2001 Apr;52(356):615-21
references.pmid: 11373309
references.citation: Ann Bot. 2002 Feb;89(2):183-9
references.pmid: 12099349
references.citation: J Exp Bot. 2007;58(4):815-25
references.pmid: 17032729
references.citation: Philos Trans R Soc Lond B Biol Sci. 2000 Oct 29;355(1402):1489-98
references.pmid: 11128002
references.citation: Annu Rev Plant Biol. 2013;64:267-91
references.pmid: 23451789
references.citation: Plant Cell Environ. 2006 Mar;29(3):340-52
references.pmid: 17080590
references.citation: Planta. 1991 Sep;185(2):255-60
references.pmid: 24186349
references.citation: Sensors (Basel). 2011;11(4):3765-79
references.pmid: 22163820
references.citation: Photosynth Res. 1993 Aug;37(2):89-102
references.pmid: 24317706
references.citation: Proc Natl Acad Sci U S A. 2004 Jan 27;101(4):918-22
references.pmid: 14732685
references.citation: Plant Cell Environ. 2006 Aug;29(8):1508-18
references.pmid: 16898014
references.citation: Funct Plant Biol. 2009 Nov;36(11):880-892
references.pmid: 32688699
references.citation: Annu Rev Plant Biol. 2008;59:89-113
references.pmid: 18444897
references.citation: Plant Physiol. 2005 Sep;139(1):254-66
references.pmid: 16113223
references.citation: Funct Plant Biol. 2007 Mar;34(2):83-94
references.pmid: 32689335
references.citation: Science. 1982 Oct 29;218(4571):443-8
references.pmid: 17808529
references.citation: Plant Physiol. 1984 Sep;76(1):266-9
references.pmid: 16663812
references.citation: Plant Cell Physiol. 2003 Dec;44(12):1290-300
references.pmid: 14701924