title: Evaluation of different methods for detecting methicillin resistance in Staphylococcus aureus isolates in a university hospital located in the Northeast of Brazil.
abstract: Many methods have been described for the detection of methicillin-resistant Staphylococcus aureus (MRSA), but the heterogeneous expression of methicillin resistance affects the reliability of these methods. The aim of the present study was to evaluate some methods for detecting methicillin resistance in Staphylococcus aureus isolates in a university hospital located in the Northeast of Brazil. Among the isolates, 15 were methicillin-susceptible and 45 were methicillin-resistant, including low-level heterogeneous resistance strains. Both the 30 ηg-cefoxitin disk and PBP2a test had 100% sensibility/specificity and appear to be good options for the detection of MRSA in the clinical laboratory.
authors.affiliation: Departamento de Microbiologia e Parasitologia, Centro de Biociências, Universidade Federal do Rio Grande do Norte , Natal, RN , Brasil.
authors.forename: Francisco Canindé
authors.initials: FC
authors.lastname: de Sousa Júnior
authors.forename: Gildelane da Silva
authors.initials: Gda S
authors.lastname: Néri
authors.forename: Ana Karine
authors.initials: AK
authors.lastname: Silva
authors.forename: Bibiana Priscila Rodrigues Câmara
authors.initials: BP
authors.lastname: de Araújo
authors.forename: Myrian Júlia
authors.initials: MJ
authors.lastname: de Paiva Dourado Guerra
authors.forename: Maria José
authors.initials: MJ
authors.lastname: de Britto Costa Fernandes
authors.forename: Eveline Pipolo
authors.initials: EP
authors.lastname: Milan
authors.forename: Maria Celeste Nunes
authors.initials: MC
authors.lastname: de Melo
keywords: MRSA; PBP2a; cefoxitin; heterogeneous resistance; methicillin resistance
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