title: EphA2 and Src regulate equatorial cell morphogenesis during lens development.
abstract: High refractive index and transparency of the eye lens require uniformly shaped and precisely aligned lens fiber cells. During lens development, equatorial epithelial cells undergo cell-to-cell alignment to form meridional rows of hexagonal cells. The mechanism that controls this morphogenesis from randomly packed cuboidal epithelial cells to highly organized hexagonal fiber cells remains unknown. In Epha2(-/-) mouse lenses, equatorial epithelial cells fail to form precisely aligned meridional rows; moreover, the lens fulcrum, where the apical tips of elongating epithelial cells constrict to form an anchor point before fiber cell differentiation and elongation at the equator, is disrupted. Phosphorylated Src-Y424 and cortactin-Y466, actin and EphA2 cluster at the vertices of wild-type hexagonal epithelial cells in organized meridional rows. However, phosphorylated Src and phosphorylated cortactin are not detected in disorganized Epha2(-/-) cells with altered F-actin distribution. E-cadherin junctions, which are normally located at the basal-lateral ends of equatorial epithelial cells and are diminished in newly differentiating fiber cells, become widely distributed in the apical, lateral and basal sides of epithelial cells and persist in differentiating fiber cells in Epha2(-/-) lenses. Src(-/-) equatorial epithelial cells also fail to form precisely aligned meridional rows and lens fulcrum. These results indicate that EphA2/Src signaling is essential for the formation of the lens fulcrum. EphA2 also regulates Src/cortactin/F-actin complexes at the vertices of hexagonal equatorial cells for cell-to-cell alignment. This mechanistic information explains how EphA2 mutations lead to disorganized lens cells that subsequently contribute to altered refractive index and cataracts in humans and mice.
authors.affiliation: School of Optometry and Vision Science Program, University of California Berkeley, Berkeley, CA 94720, USA.
authors.forename: Catherine
authors.initials: C
authors.lastname: Cheng
authors.forename: Moham M
authors.initials: MM
authors.lastname: Ansari
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Cooper
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Gong
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002386:Cataract / Q000378:metabolism; D002454:Cell Differentiation; D002465:Cell Movement; D051356:Cortactin / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D007908:Lens, Crystalline / Q000196:embryology* / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D009024:Morphogenesis; D010766:Phosphorylation; D036104:Receptor, EphA2 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D019061:src-Family Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D015820:Cadherins; D051356:Cortactin; D036104:Receptor, EphA2; D019061:src-Family Kinases
keywords: Cataracts; Eph; Ephrin; Lens
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