title: Anti-SSA Ro52/Ro60 antibody testing by immunodot could help the diagnosis of Sjogren's syndrome in the absence of anti-SSA/SSB antibodies by ELISA.
abstract: OBJECTIVE
  The objective of this study was to assess the diagnostic value of anti-Ro52/60 antibodies by immunodot in patients with suspected SS.
  
  
  METHODS
  All patients between 2008 and 2012 with suspected SS without anti-SSA/SSB antibodies by ELISA and who had a determination of anti-SSA (Ro52/Ro60) antibodies by immunodot (Euroimmun, Germany) were retrospectively analysed.
  
  
  RESULTS
  Eighty-four patients (median age 62 years; males 35 cases) were included. Forty-five patients had associated interstitial lung disease (ILD). American-European Consensus Group (AECG) criteria for SS were fulfilled in 10 patients (12%) with a positive salivary gland biopsy, and among them 2 patients had anti-Ro52 antibodies by dot (20%). Among 74 patients with a negative salivary gland biopsy, 16 (22%) had anti-Ro52/60 antibodies and 10 (14%) fulfilled AECG criteria when including Ro52/60 antibodies. A comparison of patients with and without anti-Ro52/60 antibodies revealed no differences, except gamma globulin levels and more frequent steroid use in patients with anti-Ro52/60 antibodies (P < 0.05). In 45 patients with ILD and dry eye/mouth syndrome, 10 (22%) had positive anti-Ro52/60 antibodies. In these patients 2 (4%) fulfilled AECG criteria without anti-Ro52/60 antibodies vs 4 (8%) patients if anti-Ro52/60 antibodies were included.
  
  
  CONCLUSIONS
  In patients with dry eye/mouth syndrome without anti-SSA/SSB antibodies by ELISA, the detection of anti-Ro52/Ro60 antibodies by dot could help in the diagnosis of SS. In patients with ILD, the lower frequency of a Chisholm score ≥3 enhances the interest of anti-Ro52/Ro60 screening.
authors.affiliation: Service de Médecine Interne, Hôpital Jean Verdier, Université Paris 13, AP-HP, 93140 Bondy, France. arsene.mekinian@jvr.aphp.fr.
authors.forename: Arsène
authors.initials: A
authors.lastname: Mekinian
authors.forename: Pascale
authors.initials: P
authors.lastname: Nicaise-Roland
authors.forename: Sylvie
authors.initials: S
authors.lastname: Chollet-Martin
authors.forename: Olivier
authors.initials: O
authors.lastname: Fain
authors.forename: Bruno
authors.initials: B
authors.lastname: Crestani
mesh_terms: D000368:Aged; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D001324:Autoantigens / Q000276:immunology*; D015415:Biomarkers / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D015151:Immunoblotting; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000276:immunology*; D012189:Retrospective Studies; D012261:Ribonucleoproteins / Q000276:immunology*; D012859:Sjogren's Syndrome / Q000175:diagnosis* / Q000276:immunology; D000099207:SS-A Antigen
chemical_list: D000888:Antibodies, Anti-Idiotypic; D001324:Autoantigens; D015415:Biomarkers; D010446:Peptide Fragments; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C506261:SSB protein, human
keywords: Sjogren’s syndrome; anti-Ro52/60; diagnosis; interstitial lung disease