title: The cytoplasmic domain of neuropilin-1 regulates focal adhesion turnover.
abstract: Though the vascular endothelial growth factor coreceptor neuropilin-1 (Nrp1) plays a critical role in vascular development, its precise function is not fully understood. We identified a group of novel binding partners of the cytoplasmic domain of Nrp1 that includes the focal adhesion regulator, Filamin A (FlnA). Endothelial cells (ECs) expressing a Nrp1 mutant devoid of the cytoplasmic domain (nrp1(cyto)(Δ/Δ)) migrated significantly slower in response to VEGF relative to the cells expressing wild-type Nrp1 (nrp1(+/+) cells). The rate of FA turnover in VEGF-treated nrp1(cyto)(Δ/Δ) ECs was an order of magnitude lower in comparison to nrp1(+/+) ECs, thus accounting for the slower migration rate of the nrp1(cyto)(Δ/Δ) ECs.
authors.affiliation: Department of Molecular Cardiology, Lerner Research Institute, the Cleveland Clinic, Cleveland, OH 44195, United States.
authors.forename: Himabindu Reddy
authors.initials: HR
authors.lastname: Seerapu
authors.forename: Susmita
authors.initials: S
authors.lastname: Borthakur
authors.forename: Nathan
authors.initials: N
authors.lastname: Kong
authors.forename: Sudesh
authors.initials: S
authors.lastname: Agrawal
authors.forename: Judy
authors.initials: J
authors.lastname: Drazba
authors.forename: Amit
authors.initials: A
authors.lastname: Vasanji
authors.forename: Alessandro
authors.initials: A
authors.lastname: Fantin
authors.forename: Christiana
authors.initials: C
authors.lastname: Ruhrberg
authors.forename: Matthias
authors.initials: M
authors.lastname: Buck
authors.forename: Arie
authors.initials: A
authors.lastname: Horowitz
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D003593:Cytoplasm / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D064448:Filamins / Q000737:chemistry / Q000378:metabolism; D022001:Focal Adhesions / Q000378:metabolism*; D051379:Mice; D009154:Mutation; D039942:Neuropilin-1 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D064448:Filamins; D042461:Vascular Endothelial Growth Factor A; D039942:Neuropilin-1
keywords: Cytoplasmic domain; Filamin A; Focal adhesion; Neuropilin-1
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