title: Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method.
abstract: Diagnosis of leptospirosis by PCR is hampered due to the presence of substances on biological fluids. Here, we report an immunomagnetic separation step prior to PCR which improved the detection of Leptospira spp. in blood and urine samples from dogs. It resulted in a significant improvement on sensitivity for diagnosis of canine leptospirosis.
authors.affiliation: Laboratório de Imunologia Aplicada, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas , Pelotas, RS , Brasil ; Laboratório de Imunodiagnóstico, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas , Pelotas, RS , Brasil.
authors.forename: Leonardo Garcia
authors.initials: LG
authors.lastname: Monte
authors.forename: Sérgio
authors.initials: S
authors.lastname: Jorge
authors.forename: João Paulo Mesquita
authors.initials: JP
authors.lastname: Luiz
authors.forename: Francine
authors.initials: F
authors.lastname: Sinnott
authors.forename: Kömmling Seixas
authors.initials: KS
authors.lastname: Seixas
authors.forename: José Antonio Guimarães
authors.initials: JA
authors.lastname: Aleixo
authors.forename: Luis Ernesto
authors.initials: LE
authors.lastname: Samartino
authors.forename: Fabricio Rochedo
authors.initials: FR
authors.lastname: Conceição
authors.forename: Cláudia Pinho
authors.initials: CP
authors.lastname: Hartleben
keywords: Canine leptospirosis; IMS-PCR; LipL32
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