title: Pharmacokinetics study of calf thymus DNA in rats and beagle dogs with (3)H-labeling and tracing method.
abstract: This study developed a radioisotope detection and tracing method to investigate the pharmacokinetic properties of calf thymus DNA (ctDNA) in rats and beagle dogs. The radioactivity labeling result was detected through gel electrophoresis analysis, and pharmacokinetic analytical methods for (3)H-ctDNA in rat and beagle dog plasma were developed, respectively. Full method validation indicated that the established radioisotope method was sensitive, specific, rapid and reliable, and the results were all in accordance with the analysis requirement in biological samples. After intravenous administration of the planned doses of (3)H-ctDNA to the rats and beagle dogs, plasma concentrations from the various dose groups declined rapidly. In addition, the radioactive concentration of (3)H-ctDNA in the plasma from single and multiple dosings decreased in a similar trend. Through comparative analysis of the pharmacokinetic parameters, we inferred that the elimination of ctDNA accorded with the linear pharmacokinetic characteristic. The results demonstrated that ctDNA was rapidly eliminated in rat and beagle dog plasma and would not accumulate, indicating the safe use of ctDNA as an immunoadsorptive material without bringing out potential risk.
authors.affiliation: College of Bioengineering, Henan University of Technology, Zhengzhou, PR China; Center of Drug Metabolism and Pharmacokinetics, College of Pharmacy, China Pharmaceutical University, Nanjing, PR China.
authors.forename: Shuoye
authors.initials: S
authors.lastname: Yang
authors.forename: Amer
authors.initials: A
authors.lastname: Talbi
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Hanlin
authors.initials: H
authors.lastname: Song
authors.forename: Xijing
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D001682:Biological Availability; D002138:Calibration; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000493:pharmacokinetics*; D004285:Dogs; D005260:Female; D007163:Immunosorbent Techniques; D007275:Injections, Intravenous; D008297:Male; D011786:Quality Control; D011868:Radioisotopes / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013045:Species Specificity; D014316:Tritium / Q000032:analysis*
chemical_list: D011868:Radioisotopes; D014316:Tritium; D004247:DNA; C427203:calf thymus DNA
keywords: Analytical method; Calf thymus DNA; Pharmacokinetics; Radioisotope labeling and detection; Tritium