title: Extended-spectrum beta-lactamase-producing bacteria isolated from hematologic patients in Manaus, State of Amazonas, Brazil.
abstract: Antibiotic therapy in hematologic patients, often weak and susceptible to a wide range of infections, particularly nosocomial infections derived from long hospitalization periods, is a challenging issue. This paper presents ESBL-producing strains isolated from such hematologic patients treated at the Amazon Hematology and Hemotherapy Foundation (HEMOAM) in the Brazilian Amazon Region to identify the ESBL genes carried by them as well as the susceptibility to 11 antimicrobial agents using the E-test method. A total of 146 clinical samples were obtained from July 2007 to August 2008, when 17 gram-negative strains were isolated in our institution. The most frequent isolates confirmed by biochemical tests and 16S rRNA sequencing were E. coli (8/17), Serratia spp. (3/17) and B.cepacia (2/17). All gram-negative strains were tested for extended-spectrum-beta-lactamases (ESBLs), where: (12/17) strains carried ESBL; among these, (8/12) isolates carried bla TEM, bla CTX-M, bla OXA , bla SHV genes, (1/12) bla TEM gene and (3/12) bla TEM, bla CTX-M, bla OXA genes. Antibiotic resistance was found in (15/17) of the isolates for tetracycline, (12/17) for ciprofloxacin, (1/17) resistance for cefoxitin and chloramphenicol, (1/17) for amikacin and (3/17) cefepime. This research showed the presence of gram-negative ESBL-producing bacteria infecting hematologic patients in HEMOAM. These strains carried the bla TEM, bla SHV, bla CTX-M and bla OXA genes and were resistant to different antibiotics used in the treatment. This finding was based on a period of 13 months, during which clinical samples from specific populations were obtained. Therefore, caution is required when generalizing the results that must be based on posological orientations and new breakpoints for disk diffusion and microdilution published by CLSI 2010.
authors.affiliation: Universidade do Estado do Amazonas, Fundação de Medicina Tropical do Amazonas, Fundação de Hematologia e Hemoterapia do Amazonas , Manaus, AM , Brasil.
authors.forename: Cristina Motta
authors.initials: CM
authors.lastname: Ferreira
authors.forename: William Antunes
authors.initials: WA
authors.lastname: Ferreira
authors.forename: Nayanne Cristina Oliveira da Silva
authors.initials: NC
authors.lastname: Almeida
authors.forename: Felipe Gomes
authors.initials: FG
authors.lastname: Naveca
authors.forename: Maria das Graças Vale
authors.initials: Md
authors.lastname: Barbosa
keywords: CTX-M; ESBL; OXA; SHV; TEM; beta-lactams; nosocomial infection
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