title: Modulation of nicotinic acetylcholine receptors in native sympathetic neurons by ethanol.
abstract: OBJECTIVE
  To determine whether actions on nicotine acetylcholine receptors (nAChRs) contribute to ethanol's depressant effects on the autonomic nervous system.
  
  
  METHODS
  The acute effects of ethanol on nAChRs were examined in primary cultured superior cervical ganglion (SCGs) by whole-cell patch clamp recordings. After the whole-cell configuration was formed, drugs diluted to various concentrations with extracellular solution were applied directly to single neurons.
  
  
  RESULTS
  Held at -70 mV, ethanol significantly and reversibly inhibited nicotine-evoked currents (INic) with a maximum inhibition rate of ~80% and an IC50 of 232.88±40.66 mM. At 50 mM, ethanol accelerated the slow decay, but did not affect the quick decay and rising time of INic. There was neither use-dependence nor voltage-dependence of ethanol on suppressing INic in SCGs.
  
  
  CONCLUSIONS
  Ethanol inhibited the whole-cell INic significantly,probably through noncompetitive inhibition at the binding sites outside of the cell membrane.
authors.affiliation: Department of Neurology, Institution of Neuropsychiatry Research, Renmin Hospital of Wuhan University Jiefang Road 238, Wuhan 430060, Hubei, PR China.
authors.forename: Zheman
authors.initials: Z
authors.lastname: Xiao
authors.forename: Fan
authors.initials: F
authors.lastname: Zhu
authors.forename: Zuneng
authors.initials: Z
authors.lastname: Lu
authors.forename: Songqing
authors.initials: S
authors.lastname: Pan
authors.forename: Jingjing
authors.initials: J
authors.lastname: Liang
authors.forename: Jiangquan
authors.initials: J
authors.lastname: Zheng
authors.forename: Zhongchun
authors.initials: Z
authors.lastname: Liu
keywords: Ethanol; desensitization; nicotine; nicotine acetylcholine receptors; superior cervical ganglion
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