title: Rapid detection of Akabane virus by a novel reverse transcription loop-mediated isothermal amplification assay (RT-LAMP).
abstract: BACKGROUND
  Akabane disease, caused by Akabane virus, is an insect-transmitted disease of ruminants that is primarily characterized by fetal damage.
  
  
  RESULTS
  In this study, a novel reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of Akabane virus was successfully developed. The primers were designed to target the highly conserved fragment of nucleoprotein from the Akabane virus. The results indicate that the assay is highly specific and sensitive with a detection limit of 5.0 TCID50/mL within a 60-min incubation time. A total of 126 abortive samples collected from Xinjiang province were detected by the established RT-LAMP. The results of RT-LAMP assay showed 96.8% agreement with the semi-nested RT-PCR.
  
  
  CONCLUSIONS
  This study is to first to develop a rapid, sensitive, and accurate method for the detection of Akabane virus, which may be used to screen clinical samples in developing countries or regions.
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, People's Republic of China. xjmqlqj@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Qiao
authors.forename: Junwei
authors.initials: J
authors.lastname: Wang
authors.forename: Qingling
authors.initials: Q
authors.lastname: Meng
authors.forename: Guochao
authors.initials: G
authors.lastname: Wang
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhihao
authors.initials: Z
authors.lastname: He
authors.forename: Haibo
authors.initials: H
authors.lastname: Yang
authors.forename: Zaichao
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xuepeng
authors.initials: X
authors.lastname: Cai
authors.forename: Chuangfu
authors.initials: C
authors.lastname: Chen
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D007313:Insecta; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016851:Orthobunyavirus / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification*; D012377:Rodentia; D012680:Sensitivity and Specificity; D013997:Time Factors; D014730:Veterinary Medicine / Q000379:methods*; D014773:Virology / Q000379:methods*
chemical_list: D017931:DNA Primers; D012367:RNA, Viral
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