title: Evaluation and biochemical characterization of a distinctive pyoverdin from a pseudomonas isolated from chickpea rhizosphere.
abstract: Microbial siderophores confiscate the available ferric ions around the roots and trigger a reaction resulting in plant growth promotion. In our study, a high level of siderophore production was observed from a newly isolated Pseudomonas sp. from the rhizosphere of Chickpea plants. Under an iron depleted condition in Standard Succinic acid medium a 1000 μgmL(-1) of siderophore production was achieved. Increasing the concentration of iron showed an inverse relationship between growth and siderophore production. Fourier Transform Infrared Spectroscopy (FTIR) analysis of the purified crystals, its UV spectral analysis and High Pressure Liquid Chromatography (HPLC) revealed the identity of the siderophore as similar to that of pyoverdin with distinctive characters. Electron spray ionization mass spectroscopy (ESIMS) shows presence of abundance of A1 ions (419 m/z) and branching of amino acids from B1-B5. This pyoverdin contains a cyclic tetra peptide but Serine and Arginine are missing. Based on our analysis and deviations from the reported structure of pyoverdin it is suggested that this pseudomonas produces distinctly characterized pyoverdin siderophore.
authors.affiliation: Department of Microbiology, University School of Sciences, Gujarat University of Sciences, Gujarat University , Ahmedabad 380 009, Gujarat , India.
authors.forename: Neelam
authors.initials: N
authors.lastname: Tank
authors.forename: Narayanan
authors.initials: N
authors.lastname: Rajendran
authors.forename: Baldev
authors.initials: B
authors.lastname: Patel
authors.forename: Meenu
authors.initials: M
authors.lastname: Saraf
keywords: Fluorescense; Pseudomonas; collision activation; hydroxamates; pyoverdin
references.citation: Rev Latinoam Microbiol. 2002 Jul-Dec;44(3-4):112-7
references.pmid: 17061484
references.citation: Appl Environ Microbiol. 2003 Feb;69(2):1143-53
references.pmid: 12571041
references.citation: Appl Environ Microbiol. 2000 Jan;66(1):325-31
references.pmid: 10618243
references.citation: Appl Environ Microbiol. 2001 Apr;67(4):1718-27
references.pmid: 11282626
references.citation: Nat Prod Rep. 2010 May;27(5):637-57
references.pmid: 20376388
references.citation: Anal Biochem. 1987 Jan;160(1):47-56
references.pmid: 2952030
references.citation: Appl Environ Microbiol. 2001 Sep;67(9):3934-42
references.pmid: 11525988
references.citation: Microbiol Res. 2003;158(1):77-81
references.pmid: 12608583
references.citation: J Bacteriol. 1953 Jan;65(1):45-7
references.pmid: 13022627
references.citation: Z Naturforsch C J Biosci. 2002 Jan-Feb;57(1-2):9-16
references.pmid: 11926550
references.citation: Appl Environ Microbiol. 1995 Nov;61(11):3769-74
references.pmid: 16535157
references.citation: FEMS Microbiol Rev. 1993 Apr;10(3-4):209-28
references.pmid: 8318257