title: RNA structure experimental analysis--chemical modification.
abstract: The purpose of this protocol is to identify 'footprints' of protein on RNA. However, it can also be used to analyze the secondary structure of RNA. This protocol is optimized for large RNA molecules; however, it can be adapted for the study of small RNAs.
authors.affiliation: Department of Biology, University of Rochester, Rochester, NY, USA.
authors.forename: Zhili
authors.initials: Z
authors.lastname: Xu
authors.forename: Gloria
authors.initials: G
authors.lastname: Culver
mesh_terms: D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry* / Q000302:isolation & purification; D012261:Ribonucleoproteins / Q000032:analysis*; D013463:Sulfuric Acid Esters / Q000737:chemistry
chemical_list: D012261:Ribonucleoproteins; D013463:Sulfuric Acid Esters; D012313:RNA; C007482:dimethyl sulfate
keywords: Dimethyl sulfate (DMS); Primer extension; Quantification and normalization RNA; RNA extraction; RNA structure experimental analysis; Ribonucleoprotein (RNP)