title: A DNA sequence element that advances replication origin activation time in Saccharomyces cerevisiae.
abstract: Eukaryotic origins of DNA replication undergo activation at various times in S-phase, allowing the genome to be duplicated in a temporally staggered fashion. In the budding yeast Saccharomyces cerevisiae, the activation times of individual origins are not intrinsic to those origins but are instead governed by surrounding sequences. Currently, there are two examples of DNA sequences that are known to advance origin activation time, centromeres and forkhead transcription factor binding sites. By combining deletion and linker scanning mutational analysis with two-dimensional gel electrophoresis to measure fork direction in the context of a two-origin plasmid, we have identified and characterized a 19- to 23-bp and a larger 584-bp DNA sequence that are capable of advancing origin activation time.
authors.affiliation: Molecular and Cellular Biology Program, University of Washington, Seattle, Washington 98195.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Pohl
authors.forename: Katherine
authors.initials: K
authors.lastname: Kolor
authors.forename: Walton L
authors.initials: WL
authors.lastname: Fangman
authors.forename: Bonita J
authors.initials: BJ
authors.lastname: Brewer
authors.forename: M K
authors.initials: MK
authors.lastname: Raghuraman
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002503:Centromere / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D016296:Mutagenesis; D018741:Replication Origin / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
chemical_list: C064055:ABFI protein, S cerevisiae; D004268:DNA-Binding Proteins; D051858:Forkhead Transcription Factors; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C554167:URA3 protein, S cerevisiae; D004247:DNA
keywords: ARS; DNA replication; Saccharomyces cerevisiae; origins of replication; temporal regulation
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