title: Apoptosis-related gene expression in glioblastoma (LN-18) and medulloblastoma (Daoy) cell lines.
abstract: The expression of apoptosis genes in a commercial pre-designed low-density array from Applied Biosystems was evaluated in two human brain cancer cell models, LN-18 and Daoy (HTB-186™) in comparison to the reference human primary endothelial cells under basic conditions. Analysis of the gene expression in the cancer cell lines compared to the normal control revealed features reflecting anti-apoptotic and inflammatory characteristics of the former. There was an overall downregulation of apoptosis-stimulating genes in both cancer cell lines, along with an upregulation of certain apoptosis inhibitors. A number of genes demonstrated statistically significant changes in their expressions, including BAX (BCL2-associated X protein); the CARD4/NLR family, CARD domain containing 4; CASP10 (caspase 10, apoptosis-related cysteine peptidase); DAP1 (death-associated protein kinase 1), and BIRC5 (baculoviral IAP repeat-containing 5). Anti-apoptotic potential in both cell lines was demonstrated by changes in the Bax:Bcl-2 ratio and downregulation of the APAF1 gene in LN18 cells. There was also significant downregulation of extrinsic signals and the TNF/FADD/inflammatory cascade, and upregulation of caspase inhibitors (IAPs). These results provided a novel molecular characterization of important human cancer cell lines, which might provide a useful research tool for investigating the experimental model of the CNS cell.
authors.affiliation: Department of Genetic Diagnostics and Nutrigenomics, Chair of Clinical Biochemistry, The Jagiellonian University, Medical College, Kraków, Poland, mbwybran@cyf-kr.edu.pl.
authors.forename: Iwona
authors.initials: I
authors.lastname: Wybranska
authors.forename: Anna
authors.initials: A
authors.lastname: Polus
authors.forename: Magdalena
authors.initials: M
authors.lastname: Mikolajczyk
authors.forename: Anna
authors.initials: A
authors.lastname: Knapp
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Sliwa
authors.forename: Barbara
authors.initials: B
authors.lastname: Zapala
authors.forename: Teresa
authors.initials: T
authors.lastname: Staszel
authors.forename: Aldona
authors.initials: A
authors.lastname: Dembinska-Kiec
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics; D053477:Apoptotic Protease-Activating Factor 1 / Q000235:genetics; D053455:Caspase 10 / Q000235:genetics; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000235:genetics*; D053200:Fas-Associated Death Domain Protein / Q000235:genetics; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics; D008527:Medulloblastoma / Q000235:genetics* / Q000473:pathology*; D053474:Nod1 Signaling Adaptor Protein / Q000235:genetics; D000077022:Survivin; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics*; D051028:bcl-2-Associated X Protein / Q000235:genetics
chemical_list: C506592:APAF1 protein, human; D051017:Apoptosis Regulatory Proteins; D053477:Apoptotic Protease-Activating Factor 1; C107489:BIRC5 protein, human; C091572:DAP protein, human; C505533:FADD protein, human; D053200:Fas-Associated Death Domain Protein; D051034:Inhibitor of Apoptosis Proteins; C119067:NOD1 protein, human; D053474:Nod1 Signaling Adaptor Protein; D000077022:Survivin; D014409:Tumor Necrosis Factor-alpha; D051028:bcl-2-Associated X Protein; D053455:Caspase 10; C506438:CASP10 protein, human
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