title: Real-time DNP NMR observations of acetic acid uptake, intracellular acidification, and of consequences for glycolysis and alcoholic fermentation in yeast.
abstract: Uptake and upshot in vivo: Straightforward methods that permit the real-time observation of organic acid influx, intracellular acidification, and concomitant effects on cellular-reaction networks are crucial for improved bioprocess monitoring and control. Herein, dynamic nuclear polarization (DNP) NMR is used to observe acetate influx, ensuing intracellular acidification and the metabolic consequences on alcoholic fermentation and glycolysis in living cells.
authors.affiliation: Albeda Research, Gamle Carlsberg Vej 10, 1799 Copenhagen V (Denmark).
authors.forename: Pernille R
authors.initials: PR
authors.lastname: Jensen
authors.forename: Magnus
authors.initials: M
authors.lastname: Karlsson
authors.forename: Mathilde H
authors.initials: MH
authors.lastname: Lerche
authors.forename: Sebastian
authors.initials: S
authors.lastname: Meier
mesh_terms: D019342:Acetic Acid / Q000737:chemistry* / Q000378:metabolism*; D005285:Fermentation; D006019:Glycolysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000378:metabolism*; D015003:Yeasts / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D019342:Acetic Acid
keywords: NMR spectroscopy; acetate; bioethanol; in vivo spectroscopy; yeast fermentation