title: An ultrasensitive homogeneous chemiluminescent assay for microRNAs.
abstract: On the basis of a dual-amplification strategy, microRNA (miRNA) hybridization amplification by a duplex-specific nuclease (DSN) and signal amplification by a DNAzyme, a highly sensitive and selective homogeneous miRNA assay is developed. By leveraging on the cumulative and selective nature of the DSN amplification process, excellent sensitivity and selectivity are accomplished.
authors.affiliation: Department of Chemistry, National University of Singapore, Singapore 117543. chmgaoz@nus.edu.sg.
authors.forename: Huimin
authors.initials: H
authors.lastname: Deng
authors.forename: Yuqian
authors.initials: Y
authors.lastname: Ren
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Gao
mesh_terms: D002623:Chemistry Techniques, Analytical; D057230:Limit of Detection; D008163:Luminescent Measurements; D035683:MicroRNAs / Q000737:chemistry*
chemical_list: D035683:MicroRNAs