title: Calmodulin activation of polo-like kinase 1 is required during mitotic entry.
abstract: Polo-like kinase 1 (Plk1) is a conserved key regulator of the G2/M transition, but its upstream spatiotemporal regulators remain unknown. With the help of immunofluorescence, co-immunoprecipitation, and glutathione S-transferase (GST) pull-down assay, we found that calmodulin (CaM) is one such regulatory molecule that associates with Plk1 from G2 to metaphase. More importantly, this interaction results in considerable stimulation of Plk1 kinase activity leading to hyperphosphorylation of Cdc25C. Our results provide new insight into the role of CaM as an upstream regulator of Plk1 activation during mitotic entry.
authors.affiliation: a Model Animal Research Center (MARC) and School of Medicine, Nanjing University, Nanjing 210093, China.
authors.forename: Gu
authors.initials: G
authors.lastname: Dai
authors.forename: Yan
authors.initials: Y
authors.lastname: Qian
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Fan-Li
authors.initials: FL
authors.lastname: Meng
authors.forename: Fei-Yan
authors.initials: FY
authors.lastname: Pan
authors.forename: Wei-Gan
authors.initials: WG
authors.lastname: Shen
authors.forename: Sheng-Zhou
authors.initials: SZ
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Xue
authors.forename: Chao-Jun
authors.initials: CJ
authors.lastname: Li
mesh_terms: D002147:Calmodulin / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002455:Cell Division / Q000235:genetics*; D002460:Cell Line; D018385:Centrosome / Q000378:metabolism; D004789:Enzyme Activation; D016195:G2 Phase; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics; D020687:cdc25 Phosphatases / Q000378:metabolism*; D000097869:Polo-Like Kinase 1
chemical_list: D002147:Calmodulin; D018797:Cell Cycle Proteins; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D020687:cdc25 Phosphatases; D000097869:Polo-Like Kinase 1; C517009:CDC25C protein, human