title: Preservation of cell viability and protein conformation on immobilization within nanofibers via electrospinning functionalized yeast.
abstract: We investigate the immobilization of a model system of functionalized yeast that surface-display enhanced green fluorescent protein (eGFP) within chemically crosslinked polyvinyl alcohol (PVA) nanofibers. Yeast is incorporated into water insoluble nanofibrous materials by direct electrospinning with PVA followed by vapor phase chemical crosslinking of the polymer. Incorporation of yeast into the fibers is confirmed by elemental analysis and the viability is indicated by live/dead staining. Following electrospinning and crosslinking, we confirm that the yeast maintains its viability as well as the ability to express eGFP in the correct conformation. This method of processing functionalized yeast may thus be a powerful tool in the direct immobilization of properly folded, active enzymes within electrospun nanofibers with potential applications in biocatalysis.
authors.affiliation: Fiber and Polymer Science, College of Textiles, and ‡Chemical & Biomolecular Engineering, North Carolina State University , Raleigh, North Carolina 27695, United States.
authors.forename: M Fatih
authors.initials: MF
authors.lastname: Canbolat
authors.forename: Nimish
authors.initials: N
authors.lastname: Gera
authors.forename: Christina
authors.initials: C
authors.lastname: Tang
authors.forename: Brinda
authors.initials: B
authors.lastname: Monian
authors.forename: Balaji M
authors.initials: BM
authors.lastname: Rao
authors.forename: Behnam
authors.initials: B
authors.lastname: Pourdeyhimi
authors.forename: Saad A
authors.initials: SA
authors.lastname: Khan
mesh_terms: D055162:Biocatalysis; D002470:Cell Survival / Q000187:drug effects; D049452:Green Fluorescent Proteins; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D011142:Polyvinyl Alcohol / Q000737:chemistry* / Q000494:pharmacology; D011487:Protein Conformation / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development; D014867:Water / Q000737:chemistry
chemical_list: D011108:Polymers; D014867:Water; D049452:Green Fluorescent Proteins; D011142:Polyvinyl Alcohol