title: Isolation of Microsporum gypseum in soil samples from different geographical regions of brazil, evaluation of the extracellular proteolytic enzymes activities (keratinase and elastase) and molecular sequencing of selected strains.
abstract: A survey of Microsporum gypseum was conducted in soil samples in different geographical regions of Brazil. The isolation of dermatophyte from soil samples was performed by hair baiting technique and the species were identified by morphology studies. We analyzed 692 soil samples and the recuperating rate was 19.2%. The activities of keratinase and elastase were quantitatively performed in 138 samples. The sequencing of the ITS region of rDNA was performed in representatives samples. M. gypseum isolates showed significant quantitative differences in the expression of both keratinase and elastase, but no significant correlation was observed between these enzymes. The sequencing of the representative samples revealed the presence of two teleomorphic species of M. gypseum (Arthroderma gypseum and A. incurvatum). The enzymatic activities may play an important role in the pathogenicity and a probable adaptation of this fungus to the animal parasitism. Using the phenotypical and molecular analysis, the Microsporum identification and their teleomorphic states will provide a useful and reliable identification system.
authors.affiliation: Instituto de Ciências Biomédicas, Departamento de Microbiologia, Universidade de São Paulo , SP , Brasil ; Laboratório de Bacteriologia, Laboratório de Investigação Médica/54, Hospital das Clínicas, Faculdade de Medicina, Instituto de Medicina Tropical, Universidade de São Paulo , São Paulo, SP , Brasil.
authors.forename: Mauro Cintra
authors.initials: MC
authors.lastname: Giudice
authors.forename: Adriana Araújo
authors.initials: AA
authors.lastname: Reis-Menezes
authors.forename: Glauce Mary Gomes
authors.initials: GM
authors.lastname: Rittner
authors.forename: Adolfo José
authors.initials: AJ
authors.lastname: Mota
authors.forename: Walderez
authors.initials: W
authors.lastname: Gambale
keywords: Brazil; Elastase; Keratinase; Microsporum gypseum; Sequencing
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