title: Evaluation of antifungal activity in essential oil of the Syzygium aromaticum (L.) by extraction, purification and analysis of its main component eugenol.
abstract: Antifungal properties of some essential oils have been well documented. Clove oil is reported to have strong antifungal activity against many fungal species. In this study we have evaluated antifungal potential of essential oil of Syzygium aromaticum (L.) against some common fungal pathogens of plants and animals namely, Fusarium moniliforme NCIM 1100, Fusarium oxysporum MTCC 284, Aspergillus sp., Mucor sp., Trichophyton rubrum and Microsporum gypseum. All fungal species were found to be inhibited by the oil when tested through agar well diffusion method. Minimum inhibitory concentration (MIC) was determined for all the species. Column chromatography was performed to separate the eugenol rich fraction from clove oil. Out of seven fractions maximum activity was obtained in column fraction II. TLC and HPLC data confirmed presence of considerable Eugenol in fraction II and clove oil. Microscopic study on effect of clove oil and column fraction II on spores of Mucor sp. and M. gypseum showed distortion and shrinkage while it was absent in other column fractions. So it can be concluded that the antifungal action of clove oil is due to its high eugenol content.
authors.affiliation: Research & Development Center, Kilpest India Ltd. , Govindpura, Bhopal-462023 , India.
authors.forename: Inder Singh
authors.initials: IS
authors.lastname: Rana
authors.forename: Aarti Singh
authors.initials: AS
authors.lastname: Rana
authors.forename: Ram Charan
authors.initials: RC
authors.lastname: Rajak
keywords: Antifungal; Chromatography; Essential oil; Eugenol; Syzygium aromaticum
references.citation: J Nat Prod. 1992 Jul;55(7):999-1003
references.pmid: 1402962
references.citation: Bioresour Technol. 2008 Jul;99(11):5145-9
references.pmid: 17945485
references.citation: J Ethnopharmacol. 1993 Aug;39(3):167-70
references.pmid: 8258973
references.citation: J Appl Microbiol. 1999 Jun;86(6):985-90
references.pmid: 10438227
references.citation: J Food Prot. 2001 Jul;64(7):1025-9
references.pmid: 11456187
references.citation: J Pharm Biomed Anal. 2005 Mar 9;37(3):597-601
references.pmid: 15740922
references.citation: J Nat Prod. 1996 Oct;59(10):987-90
references.pmid: 8904847
references.citation: J Microbiol. 2007 Oct;45(5):460-5
references.pmid: 17978807
references.citation: J Ethnopharmacol. 1998 Mar;60(2):125-31
references.pmid: 9582002
references.citation: Braz J Infect Dis. 2004 Jun;8(3):217-26
references.pmid: 15476054
references.citation: J Med Microbiol. 2009 Nov;58(Pt 11):1454-1462
references.pmid: 19589904
references.citation: Biol Pharm Bull. 2007 Jan;30(1):184-8
references.pmid: 17202684
references.citation: J Drug Target. 2005 Dec;13(10):555-61
references.pmid: 16390816