title: Transcription of interferon stimulated genes in response to Porcine rubulavirus infection in vitro.
abstract: Porcine rubulavirus (PoRV) is an emerging virus causing meningo-encephalitis and reproductive failures in pigs. Little is known about the pathogenesis and immune evasion of this virus; therefore research on the mechanisms underlying tissue damage during infection is essential. To explore these mechanisms, the effect of PoRV on the transcription of interferon (IFN) pathway members was analyzed in vitro by semi-quantitative RT-PCR. Ten TCID50 of PoRV stimulated transcription of IFNα, IFNβ, STAT1, STAT2, p48 and OAS genes in neuroblastoma cells, whereas infection with 100 TCID50 did not stimulate transcription levels more than non-infected cells. When the cells were primed with IFNα, infection with 1 TCDI50 of PoRV sufficed to stimulate the transcription of the same genes, but 10 and 100 TCID50 did not modify the transcription level of those genes as compared with non-infected and primed controls. MxA gene transcription was observed only when the cells were primed with IFNα and stimulated with 10 TCID50, whereas 100 TCID50 of PoRV did not modify the MxA transcription level as compared to non-infected and primed cells. Our results show that PoRV replication at low titers stimulates the expression of IFN-responsive genes in neuroblastoma cells, and suggest that replication of PoRV at higher titers inhibits the transcription of several members of the IFN pathway. These findings may contribute to the understanding of the pathogenesis of PoRV.
authors.affiliation: Laboratorio de Biología Molecular y Virología, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social , Puebla , México.
authors.forename: María Del Rosario
authors.initials: Mdel R
authors.lastname: Flores-Ocelotl
authors.forename: Nora Hilda
authors.initials: NH
authors.lastname: Rosas-Murrieta
authors.forename: Verónica
authors.initials: V
authors.lastname: Vallejo-Ruiz
authors.forename: Julio
authors.initials: J
authors.lastname: Reyes-Leyva
authors.forename: Irma
authors.initials: I
authors.lastname: Herrera-Camacho
authors.forename: Gerardo
authors.initials: G
authors.lastname: Santos-López
keywords: Gene expression; Interferon; LPMV; Porcine rubulavirus; STAT
references.citation: Vet Immunol Immunopathol. 1998 Aug 31;64(4):367-81
references.pmid: 9764728
references.citation: Vet Rec. 1988 Jan 2;122(1):6-10
references.pmid: 3363809
references.citation: Rev Med Virol. 2002 Nov-Dec;12(6):337-57
references.pmid: 12410527
references.citation: Virology. 2002 Dec 20;304(2):160-6
references.pmid: 12504558
references.citation: Mol Cell Biol. 2000 Jul;20(13):4814-25
references.pmid: 10848607
references.citation: J Virol. 2002 Dec;76(24):12676-82
references.pmid: 12438593
references.citation: J Virol. 2001 May;75(10):4823-31
references.pmid: 11312354
references.citation: Mol Cell Biol. 1995 Jan;15(1):152-64
references.pmid: 7799921
references.citation: Arch Virol. 1986;91(3-4):221-31
references.pmid: 3778211
references.citation: Proc Natl Acad Sci U S A. 1987 Jul;84(14):5038-42
references.pmid: 3110782
references.citation: Virus Res. 1999 Dec 15;65(2):175-85
references.pmid: 10581390
references.citation: J Hum Genet. 2001;46(11):664-7
references.pmid: 11721886
references.citation: Virology. 2007 Mar 1;359(1):82-91
references.pmid: 17027894
references.citation: J Gen Virol. 2008 Jan;89(Pt 1):1-47
references.pmid: 18089727
references.citation: Am J Vet Res. 2007 Sep;68(9):988-94
references.pmid: 17764414
references.citation: Rev Biol Trop. 2008 Jun;56(2):487-99
references.pmid: 19256422
references.citation: J Gen Virol. 1990 Mar;71 ( Pt 3):609-13
references.pmid: 2313267
references.citation: Virology. 2000 Apr 10;269(2):383-90
references.pmid: 10753717
references.citation: Virus Genes. 1997;14(1):55-61
references.pmid: 9208455
references.citation: J Gen Virol. 2007 Mar;88(Pt 3):956-966
references.pmid: 17325370
references.citation: Arch Virol. 1993;133(1-2):195-200
references.pmid: 8240010
references.citation: Virology. 2007 Mar 1;359(1):190-200
references.pmid: 17049367
references.citation: Microbes Infect. 2007 Jun;9(7):864-72
references.pmid: 17533145
references.citation: Res Vet Sci. 2008 Oct;85(2):359-67
references.pmid: 18031776