title: Confusion, knock-down and kill of Aedes aegypti using metofluthrin in domestic settings: a powerful tool to prevent dengue transmission?
abstract: BACKGROUND
  Dengue control methods are reliant upon control of the vector, primarily Aedes aegypti. Current adulticiding methods in North Queensland include treating premises with residual synthetic pyrethroid insecticides (interior residual spraying; IRS), a laborious, intrusive task. The vapor active synthetic pyrethroid metofluthrin might offer an efficient alternative as some studies indicate that it prevents biting and has strong knockdown effects. However, its expellant and/or irritant effects, longevity, residual activity and the speed with which biting behavior is disrupted have not yet been characterized.
  
  
  METHODS
  We exposed cohorts of Cairns colony (F2-4) Ae. aegypti to rooms (17-24 m3) treated with 5% and 10% AI metofluthrin emanators. Using free-flying and caged populations we measured biting (human landing rate), expulsion through unscreened windows, knockdown and death over periods ranging between a few minutes and 24 hrs. Observations of the behavior of single female Ae. aegypti exposed to metofluthrin were also made.
  
  
  RESULTS
  Female Ae. aegypti exposed to 5% or 10% metofluthrin formulations were almost entirely inhibited from biting. This was the result of rapid knockdown and mortality (80-90% in less than one hour) and to the behavioral impacts of exposure that, within minutes, caused female Ae. aegypti to become disoriented, stop landing on hosts, and seek resting sites. Exposed mosquitoes did not exhibit any increased propensity to exit treated rooms and the 10% AI resin remained fully active for at least 20 days.
  
  
  CONCLUSIONS
  The new, high-dose, resin formulations of metofluthrin act quickly to prevent biting and to knockdown and kill free-flying female Ae. aegypti in our experimental rooms. There was no evidence that metofluthrin induced escape from treated areas. Resin-based metofluthrin emanators show great potential as a replacement for labor intensive IRS for dengue vector control.
authors.affiliation: School of Public Health and Tropical Medicine and Rehabilitative Sciences, James Cook University, Cairns, Queensland 4870, Australia. Scott.ritchie@jcu.edu.au.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Ritchie
authors.forename: Gregor J
authors.initials: GJ
authors.lastname: Devine
mesh_terms: D000330:Aedes / Q000187:drug effects*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D003521:Cyclopropanes / Q000494:pharmacology*; D003715:Dengue / Q000517:prevention & control / Q000635:transmission; D005260:Female; D005464:Fluorobenzenes / Q000494:pharmacology*; D007303:Insect Vectors; D007306:Insecticides / Q000494:pharmacology*; D009032:Mosquito Control / Q000379:methods*; D011793:Queensland; D016019:Survival Analysis
chemical_list: D003521:Cyclopropanes; D005464:Fluorobenzenes; D007306:Insecticides; C492600:(2,3,5,6-tetrafluoro-4-methoxymethylphenyl)methyl-2,2-dimethyl-3-(1-propenyl)cyclopropanecarboxylate
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