title: Detection and source tracking of Escherichia coli, harboring intimin and Shiga toxin genes, isolated from the Little Bighorn River, Montana.
abstract: The Little Bighorn River flows through the Crow Indian Reservation in Montana. In 2008, Escherichia coli concentrations as high as 7179 MPN/100 ml were detected in the river at the Crow Agency Water Treatment Plant intake site. During 2008, 2009, and 2012, 10 different serotypes of E. coli, including O157:H7, harboring both intimin and Shiga toxin genes were isolated from a popular swim site of the Little Bighorn River in Crow Agency. As part of a microbial source tracking study, E. coli strains were isolated from river samples as well as from manure collected from a large cattle feeding operation in the upper Little Bighorn River watershed; 23% of 167 isolates of E. coli obtained from the manure tested positive for the intimin gene. Among these manure isolates, 19 were identified as O156:H8, matching the serotype of an isolate collected from a river sampling site close to the cattle feeding area.
authors.affiliation: a Department of Microbiology , Montana State University , Bozeman , MT , USA.
authors.forename: Steve
authors.initials: S
authors.lastname: Hamner
authors.forename: Susan C
authors.initials: SC
authors.lastname: Broadaway
authors.forename: Ethan
authors.initials: E
authors.lastname: Berg
authors.forename: Sean
authors.initials: S
authors.lastname: Stettner
authors.forename: Barry H
authors.initials: BH
authors.lastname: Pyle
authors.forename: Nita
authors.initials: N
authors.lastname: Big Man
authors.forename: Joseph
authors.initials: J
authors.lastname: Old Elk
authors.forename: Margaret J
authors.initials: MJ
authors.lastname: Eggers
authors.forename: John
authors.initials: J
authors.lastname: Doyle
authors.forename: Larry
authors.initials: L
authors.lastname: Kindness
authors.forename: Brandon
authors.initials: B
authors.lastname: Good Luck
authors.forename: Timothy E
authors.initials: TE
authors.lastname: Ford
authors.forename: Anne C
authors.initials: AC
authors.lastname: Camper
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics*; D000818:Animals; D002417:Cattle; D004197:Disease Reservoirs / Q000382:microbiology*; D004784:Environmental Monitoring; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000235:genetics*; D008372:Manure / Q000382:microbiology; D009009:Montana; D045483:Rivers / Q000382:microbiology*; D022603:Shiga Toxins / Q000235:genetics*; D014774:Virulence / Q000235:genetics; D014873:Water Pollutants / Q000032:analysis*
chemical_list: D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; D008372:Manure; D022603:Shiga Toxins; D014873:Water Pollutants; C073819:eaeA protein, E coli
keywords: river water; sewage pollution; water pollutants; water-borne diseases