title: Characterization of virulence genes cagA and vacA in Helicobacter Pylori and their prevalence in gastrointestinal disorders.
abstract: Prevalence of H. pylori infection was determined using cultures of gastric biopsy samples of patients attended at the academic hospital of the Federal University of Paraná, Curitiba, Paraná, Brazil. Molecular methods were used to characterize the cagA and vacA genes from bacterial isolates associated with different diseases presented by patients. Out of a total of 81, forty-two gastric biopsy samples tested were positive for H. pylori, with a prevalence of 51.9%. No significant difference was found with regard to the gender (p=0.793) and age (p=0.183) of the patients. Genotype s1m1 vacA gene was found in 67% of the cases of peptic ulcer investigated (p=1.0), despite the limited number of patients with this disease (n=3). A correlation between the presence of less virulent strains (s2m2) and reflux esophagitis was found in the majority of the cases (45%), but without statistical significance. An association between the prevalence of cagA gene, found in 92% of isolates, and peptic ulcer was not observed (p=1.0), suggesting that this gene cannot be considered a specific marker of severity in our environment. The results reinforce the importance of conducting regional studies and the need to characterize H. pylori virulence genes associated with different diseases.
authors.affiliation: Hospital de Clínicas da Universidade Federal do Paraná , Curitiba, PR , Brasil ; Programa de Pós-Graduação em Processos Biotecnológicos da Universidade Federal do Paraná , Curitiba, PR , Brasil.
authors.forename: Laura Lúcia
authors.initials: LL
authors.lastname: Cogo
authors.forename: Cristina Leise Bastos
authors.initials: CL
authors.lastname: Monteiro
authors.forename: Keite da Silva
authors.initials: Kda S
authors.lastname: Nogueira
authors.forename: Jussara Kasuko
authors.initials: JK
authors.lastname: Palmeiro
authors.forename: Marcelo Lima
authors.initials: ML
authors.lastname: Ribeiro
authors.forename: Eloá Ramalho
authors.initials: ER
authors.lastname: de Camargo
authors.forename: Daniel Locatelli
authors.initials: DL
authors.lastname: Neves
authors.forename: Aguinaldo José
authors.initials: AJ
authors.lastname: do Nascimento
authors.forename: Libera Maria Dalla
authors.initials: LM
authors.lastname: Costa
keywords: Helicobacter pylori; cagA; vacA; virulence genes
references.citation: J Clin Microbiol. 1998 Nov;36(11):3435-7
references.pmid: 9774617
references.citation: FEMS Immunol Med Microbiol. 2003 May 25;36(3):181-5
references.pmid: 12738389
references.citation: Mem Inst Oswaldo Cruz. 2003 Sep;98(6):817-21
references.pmid: 14595461
references.citation: Epidemiol Rev. 2000;22(2):283-97
references.pmid: 11218379
references.citation: J Clin Microbiol. 2003 Aug;41(8):3737-43
references.pmid: 12904384
references.citation: FEMS Immunol Med Microbiol. 2002 Jul 12;33(3):173-8
references.pmid: 12110479
references.citation: J Clin Microbiol. 1999 Jul;37(7):2306-11
references.pmid: 10364602
references.citation: J Clin Microbiol. 1999 Jul;37(7):2274-9
references.pmid: 10364597
references.citation: Gastroenterology. 1998 Jul;115(1):58-66
references.pmid: 9649459
references.citation: J Biol Chem. 1995 Jul 28;270(30):17771-7
references.pmid: 7629077
references.citation: Microbes Infect. 2003 Jul;5(8):705-13
references.pmid: 12814771
references.citation: J Pediatr (Rio J). 2006 Sep-Oct;82(5):325-34
references.pmid: 17003940
references.citation: Rev Soc Bras Med Trop. 2002 Jul-Aug;35(4):307-10
references.pmid: 12170324
references.citation: Gastroenterology. 1997 Jan;112(1):92-9
references.pmid: 8978347
references.citation: Rev Saude Publica. 2005 Oct;39(5):847-9
references.pmid: 16254664
references.citation: J Gastroenterol Hepatol. 2000 Oct;15 Suppl:G30-3
references.pmid: 11100990
references.citation: Clin Microbiol Rev. 2006 Jul;19(3):449-90
references.pmid: 16847081
references.citation: Arq Gastroenterol. 2007 Apr-Jun;44(2):107-12
references.pmid: 17962853
references.citation: Braz J Infect Dis. 2006 Aug;10(4):254-8
references.pmid: 17293907
references.citation: Lab Invest. 1995 Dec;73(6):760-70
references.pmid: 8558837
references.citation: J Clin Microbiol. 1987 Dec;25(12):2378-9
references.pmid: 3429628