title: PBK/TOPK expression in non-small-cell lung cancer: its correlation and prognostic significance with Ki67 and p53 expression.
abstract: OBJECTIVE
  The aim of this study was to evaluate the prevalence of PBK/TOPK (PDZ-binding kinase/T-LAK cell-originated protein kinase) expression, and explore the prognostic significance of PBK/TOPK expression alone and in combination with Ki67 and p53 expression in non-small-cell lung cancer (NSCLC).
  
  
  RESULTS
  We detected PBK/TOPK expression in 30 samples of normal lung tissue, 32 lymph node metastases and 279 primary non-small-cell lung cancers by immunohistochemistry, and analysed the correlation of PBK/TOPK expression with Ki67 and p53 expression in primary tumour tissues. The results showed that PBK/TOPK expression was higher in lymph node metastases (75%) than in primary tumours (44.8%) and normal lung tissues (0%). PBK/TOPK expression was associated with histological type, lymph node metastasis, and TNM stage, and was positively correlated with Ki67 and p53 expression in NSCLC. Univariate and multivariate survival analyses showed that PBK/TOPK expression was significantly associated with an unfavourable prognosis in NSCLC. The prognosis of patients with tumours positive for both PBK/TOPK expression and Ki67 or p53 expression was also significantly unfavourable.
  
  
  CONCLUSIONS
  PBK/TOPK expression is positively correlated with Ki67 and p53 expression, and can be used as an independent prognostic factor in NSCLC.
authors.affiliation: Department of Pathology, Nanfang Hospital, Guangzhou, China; Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Lei
authors.forename: Shuguang
authors.initials: S
authors.lastname: Liu
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Qi
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yumei
authors.initials: Y
authors.lastname: Li
authors.forename: Ni
authors.initials: N
authors.lastname: Lin
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Xu
authors.forename: Chen
authors.initials: C
authors.lastname: Zhi
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Mei
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Yan
authors.forename: Liyan
authors.initials: L
authors.lastname: Wan
authors.forename: Hong
authors.initials: H
authors.lastname: Shen
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism*; D009367:Neoplasm Staging; D011379:Prognosis; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D016159:Tumor Suppressor Protein p53; D020929:Mitogen-Activated Protein Kinase Kinases; C409022:PDZ-binding kinase
keywords: Ki67; PBK/TOPK; correlation; non-small-cell lung cancer; p53