title: Sequence-specific imaging of influenza A mRNA in living infected cells using fluorescent FIT-PNA.
abstract: Significant efforts have been devoted to the development of techniques allowing the investigation of viral mRNA progression during the replication cycle. We herein describe the use of sequence-specific FIT-PNA (Forced Intercalation Peptide Nucleic Acids) probes which contain a single intercalator serving as an artificial fluorescent nucleobase. FIT-PNA probes are not degraded by enzymes, neither by nucleases nor by proteases, and provide for both high sensitivity and high target specificity under physiological conditions inside the infected living host cell.
authors.affiliation: Max Planck Institute Chemistry, Gottingen, Germany.
authors.forename: Susann
authors.initials: S
authors.lastname: Kummer
authors.forename: Andrea
authors.initials: A
authors.lastname: Knoll
authors.forename: Andreas
authors.initials: A
authors.lastname: Herrmann
authors.forename: Oliver
authors.initials: O
authors.lastname: Seitz
mesh_terms: D000818:Animals; D002642:Chick Embryo; D004285:Dogs; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009980:Influenza A virus / Q000235:genetics*; D007364:Intercalating Agents / Q000737:chemistry; D061985:Madin Darby Canine Kidney Cells; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D059010:Single-Cell Analysis
chemical_list: D005456:Fluorescent Dyes; D007364:Intercalating Agents; D020135:Peptide Nucleic Acids; D012333:RNA, Messenger; D012367:RNA, Viral