title: Checkpoint kinases and the INO80 nucleosome remodeling complex enhance global chromatin mobility in response to DNA damage.
abstract: Double-strand break repair by recombination requires a homology search. In yeast, induced breaks move significantly more than undamaged loci. To examine whether DNA damage provokes an increase in chromatin mobility generally, we tracked undamaged loci under DNA-damaging conditions. We found that the yeast checkpoint factors Mec1, Rad9, and Rad53 are required for genome-wide increases in chromatin mobility, but not the repair protein Rad51. Mec1 activation by targeted Ddc1/Ddc2 enhances chromatin mobility even in the absence of damage. Finally, the INO80 chromatin remodeler is shown to act downstream from Mec1 to increase chromatin mobility, highlighting an additional damage-related role of this nucleosome remodeling complex.
authors.affiliation: Friedrich Miescher Institute for Biomedical Research, CH-4058 Basel, Switzerland;
authors.forename: Andrew
authors.initials: A
authors.lastname: Seeber
authors.forename: Vincent
authors.initials: V
authors.lastname: Dion
authors.forename: Susan M
authors.initials: SM
authors.lastname: Gasser
mesh_terms: D059447:Cell Cycle Checkpoints / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism*; D004249:DNA Damage; D016681:Genome, Fungal / Q000235:genetics; D008954:Models, Biological; D010770:Phosphotransferases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D002843:Chromatin; C483404:INO80 complex, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D010770:Phosphotransferases
keywords: DNA damage response; INO80; Mec1; chromatin mobility; double-strand breaks
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