title: Beauveria bassiana: Quercetinase production and genetic diversity.
abstract: Beauveria bassiana genetic diversity and ability to synthesize quercetin 2,3-dioxygenase (quercetinase) were analyzed. B. bassiana isolates, obtained from Brazilian soil samples, produced quercetinase after induction using 0.5 g/L quercetin. B. bassiana ATCC 7159 (29.6 nmol/mL/min) and isolate IP 11 (27.5 nmol/ml/min) showed the best performances and IP 3a (9.5 nmol/mL/min) presented the lowest level of quercetinase activity in the culture supernatant. A high level of polymorphism was detected by random amplified polymorphic DNA (RAPD) analysis. The use of internal-transcribed-spacer ribosomal region restriction fragment length polymorphism (ITS-RFLP) did not reveal characteristic markers to differentiate isolates. However, the ITS1-5.8S-ITS2 region sequence analysis provided more information on polymorphism among the isolates, allowing them to be clustered by relative similarity into three large groups. Correlation was tested according to the Person's correlation. Data of our studies showed, that lower associations among groups, level of quercetinase production, or geographical origin could be observed. This study presents the production of a novel biocatalyst by B. bassiana and suggests the possible industrial application of this fungal species in large-scale biotechnological manufacture of quercetinase.
authors.affiliation: Laboratório de Bioconversão-LaBioCon, Faculdade de Farmácia, Universidade Federal de Goiás , Goiânia, GO , Brasil.
authors.forename: Costa
authors.initials: C
authors.lastname: Eula Maria de M B
authors.forename: Pimenta
authors.initials: P
authors.lastname: Fabiana Cristina
authors.forename: Luz
authors.initials: L
authors.lastname: Christian
authors.forename: Oliveira
authors.initials: O
authors.lastname: Valéria de
authors.forename: Oliveira
authors.initials: O
authors.lastname: Marília
authors.forename: Bueno
authors.initials: B
authors.lastname: Elda
authors.forename: Petrofeza
authors.initials: P
authors.lastname: Silvana
keywords: Beauveria bassiana; genetic diversity; quercetin-2,3-dioxygenase; quercetinase
references.citation: Genome. 2006 May;49(5):495-504
references.pmid: 16767174
references.citation: Pharmacol Ther. 2002 Nov-Dec;96(2-3):67-202
references.pmid: 12453566
references.citation: Mycol Res. 2004 Apr;108(Pt 4):441-52
references.pmid: 15209284
references.citation: Arch Biochem Biophys. 2003 Sep 1;417(1):12-7
references.pmid: 12921774
references.citation: Nat Prod Rep. 2005 Dec;22(6):672-95
references.pmid: 16311630
references.citation: Biochemistry. 2006 Jan 24;45(3):1009-16
references.pmid: 16411777
references.citation: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references.pmid: 9254694
references.citation: Mycol Res. 2005 Dec;109(Pt 12):1364-72
references.pmid: 16353636
references.citation: J Invertebr Pathol. 2008 Feb;97(2):83-90
references.pmid: 17869265
references.citation: Biochimie. 2008 May;90(5):781-9
references.pmid: 18206655
references.citation: Mol Phylogenet Evol. 2001 Jan;18(1):149-62
references.pmid: 11161752
references.citation: Proteins. 2009 Jan;74(1):18-31
references.pmid: 18561187
references.citation: Mycol Res. 2004 Sep;108(Pt 9):1070-8
references.pmid: 15506018
references.citation: FEMS Microbiol Lett. 2005 Dec 15;253(2):289-94
references.pmid: 16288837
references.citation: Protein Expr Purif. 2004 May;35(1):131-41
references.pmid: 15039076
references.citation: Biotechniques. 1989 May;7(5):514-20
references.pmid: 2699240
references.citation: Cell Mol Biol Lett. 2007;12(2):240-52
references.pmid: 17192823
references.citation: FEMS Microbiol Lett. 2003 Dec 12;229(2):249-57
references.pmid: 14680707
references.citation: Parasitol Res. 2006 Mar;98(4):324-32
references.pmid: 16374618
references.citation: Eur J Biochem. 1999 Aug;263(3):871-8
references.pmid: 10469153
references.citation: Genome Res. 1998 Mar;8(3):175-85
references.pmid: 9521921
references.citation: Biotechnol Adv. 1995;13(3):455-90
references.pmid: 14536097
references.citation: FEMS Microbiol Lett. 2008 Oct;287(1):100-7
references.pmid: 18681865
references.citation: J Mol Biol. 1991 Jun 5;219(3):555-65
references.pmid: 2051488