title: Prion protein accumulation in lipid rafts of mouse aging brain.
abstract: The cellular form of the prion protein (PrP(C)) is a normal constituent of neuronal cell membranes. The protein misfolding causes rare neurodegenerative disorders known as transmissible spongiform encephalopathies or prion diseases. These maladies can be sporadic, genetic or infectious. Sporadic prion diseases are the most common form mainly affecting aging people. In this work, we investigate the biochemical environment in which sporadic prion diseases may develop, focusing our attention on the cell membrane of neurons in the aging brain. It is well established that with aging the ratio between the most abundant lipid components of rafts undergoes a major change: while cholesterol decreases, sphingomyelin content rises. Our results indicate that the aging process modifies the compartmentalization of PrP(C). In old mice, this change favors PrP(C) accumulation in detergent-resistant membranes, particularly in hippocampi. To confirm the relationship between lipid content changes and PrP(C) translocation into detergent-resistant membranes (DRMs), we looked at PrP(C) compartmentalization in hippocampi from acid sphingomyelinase (ASM) knockout (KO) mice and synaptosomes enriched in sphingomyelin. In the presence of high sphingomyelin content, we observed a significant increase of PrP(C) in DRMS. This process is not due to higher levels of total protein and it could, in turn, favor the onset of sporadic prion diseases during aging as it increases the PrP intermolecular contacts into lipid rafts. We observed that lowering sphingomyelin in scrapie-infected cells by using fumonisin B1 led to a 50% decrease in protease-resistant PrP formation. This may suggest an involvement of PrP lipid environment in prion formation and consequently it may play a role in the onset or development of sporadic forms of prion diseases.
authors.affiliation: Laboratory of Prion Biology, Department of Neuroscience, Scuola Internazionale Superiore di Studi Avanzati (SISSA), Trieste, Italy ; Department of Human Genetics, K.U., Leuven, Leuven, Belgium.
authors.forename: Federica
authors.initials: F
authors.lastname: Agostini
authors.forename: Carlos G
authors.initials: CG
authors.lastname: Dotti
authors.forename: Azucena
authors.initials: A
authors.lastname: Pérez-Cañamás
authors.forename: Maria Dolores
authors.initials: MD
authors.lastname: Ledesma
authors.forename: Federico
authors.initials: F
authors.lastname: Benetti
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Legname
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism; D037341:Fumonisins / Q000494:pharmacology; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D008297:Male; D021962:Membrane Microdomains / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009474:Neurons / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D017830:Octoxynol / Q000494:pharmacology; D018620:PrPSc Proteins / Q000737:chemistry / Q000378:metabolism*; D061251:Primary Cell Culture; D017510:Protein Folding; D012608:Scrapie / Q000378:metabolism* / Q000473:pathology; D013108:Sphingomyelin Phosphodiesterase / Q000172:deficiency / Q000235:genetics; D013109:Sphingomyelins / Q000737:chemistry / Q000378:metabolism*; D013574:Synaptosomes / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism
chemical_list: D037341:Fumonisins; D018620:PrPSc Proteins; D013109:Sphingomyelins; C056933:fumonisin B1; D017830:Octoxynol; D002784:Cholesterol; C112980:acid sphingomyelinase-1; D013108:Sphingomyelin Phosphodiesterase
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