title: Distribution, detection of enterotoxigenic strains and antimicrobial drug susceptibility patterns of bacteroides fragilis group in diarrheic and non-diarrheic feces from brazilian infants.
abstract: Despite the importance of gastrointestinal diseases and their global distribution, affecting millions of individuals around the world, the role and antimicrobial susceptibility patterns of anaerobic bacteria such as those in the Bacteroides fragilis group (BFG) are still unclear in young children. This study investigated the occurrence and distribution of species in the BFG and enterotoxigenic strains in the fecal microbiota of children and their antimicrobial susceptibility patterns. Diarrheic (n=110) and non-diarrheic (n=65) fecal samples from children aged 0-5 years old were evaluated. BFG strains were isolated and identified by conventional biochemical, physiological and molecular approaches. Alternatively, bacteria and enterotoxigenic strains were detected directly from feces by molecular biology. Antimicrobial drug susceptibility patterns were determined by the agar dilution method according to the guidelines for isolated bacteria. BFG was detected in 64.3% of the fecal samples (55% diarrheic and 80.4% non-diarrheic), and 4.6% were enterotoxigenic. Antimicrobial resistance was observed against ampicillin, ampicillin/sulbactam, piperacillin/tazobactam, meropenem, ceftriaxone, clindamycin and chloramphenicol. The data show that these bacteria are prevalent in fecal microbiota at higher levels in healthy children. The molecular methodology was more effective in identifying the B. fragilis group when compared to the biochemical and physiological techniques. The observation of high resistance levels stimulates thoughts about the indiscriminate use of antimicrobial drugs in early infancy. Further quantitative studies are needed to gain a better understanding of the role of these bacteria in acute diarrhea in children.
authors.affiliation: Laboratório de Fisiologia e Genética Molecular Bacteriana, Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas , Universidade Federal de Juiz de Fora, Juiz de Fora, MG , Brasil.
authors.forename: Débora Paula
authors.initials: DP
authors.lastname: Ferreira
authors.forename: Vânia Lúcia
authors.initials: VL
authors.lastname: Silva
authors.forename: Danielle Aparecida
authors.initials: DA
authors.lastname: Guimarães
authors.forename: Cíntia Marques
authors.initials: CM
authors.lastname: Coelho
authors.forename: Danielle Alves Gomes
authors.initials: DA
authors.lastname: Zauli
authors.forename: Luiz Macêdo
authors.initials: LM
authors.lastname: Farias
authors.forename: Maria Auxiliadora Roque
authors.initials: MA
authors.lastname: Carvalho
authors.forename: Claudio Galuppo
authors.initials: CG
authors.lastname: Diniz
keywords: Bacteroides fragilis group; ETBF; antimicrobial susceptibility; diarrhea
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