title: Influence of chronic food deprivation on structure-function relationship of juvenile rat fast muscles.
abstract: In the present study, we analyze the influence of chronic undernutrition on protein expression, muscle fiber type composition, and fatigue resistance of the fast extensor digitorum longus (EDL) muscle of male juvenile rats (45 ± 3 days of life; n = 25 and 31 rats for control and undernourished groups, respectively). Using 2D gel electrophoresis and mass spectrometry, we identified in undernourished muscles 12 proteins up-regulated (8 proteins of the electron transport chain and the glycolytic pathway, 2 cross-bridge proteins, chaperone and signaling proteins that are related to the stress response). In contrast, one down-regulated protein related to the fast muscle contractile system and two other proteins with no changes in expression were used as charge controls. By means of COX and alkaline ATPase histochemical techniques and low-frequency fatigue protocols we determined that undernourished muscles showed a larger proportion (15% increase) of Type IIa/IId fibers (oxidative-glycolytic) at the expense of Type IIb (glycolytic) fibers (15.5% decrease) and increased fatigue resistance (55.3%). In addition, all fiber types showed a significant reduction in their cross-sectional area (slow: 64.4%; intermediate: 63.9% and fast: 61.2%). These results indicate that undernourished EDL muscles exhibit an increased expression of energy metabolic and myofibrillar proteins which are associated with the predominance of oxidative and Type IIa/IId fibers and to a higher resistance to fatigue. We propose that such alterations may act as protective and/or adaptive mechanisms that counterbalance the effect of chronic undernourishment.
authors.affiliation: Department of Physiology, Biophysics & Neurosciences, CINVESTAV, IPN, Av. Instituto Politécnico Nacional No. 2508, Col. San Pedro Zacatenco, C.P. 07360, Mexico City, Mexico.
authors.forename: Azucena
authors.initials: A
authors.lastname: Ruiz-Rosado
authors.forename: Héctor A
authors.initials: HA
authors.lastname: Cabrera-Fuentes
authors.forename: Cecilia
authors.initials: C
authors.lastname: González-Calixto
authors.forename: Lorena
authors.initials: L
authors.lastname: González-López
authors.forename: Febe E
authors.initials: FE
authors.lastname: Cázares-Raga
authors.forename: Bertha
authors.initials: B
authors.lastname: Segura-Alegría
authors.forename: Günter
authors.initials: G
authors.lastname: Lochnit
authors.forename: Fidel
authors.initials: F
authors.lastname: de la Cruz Hernández-Hernández
authors.forename: Klaus T
authors.initials: KT
authors.lastname: Preissner
authors.forename: Ismael
authors.initials: I
authors.lastname: Jiménez-Estrada
mesh_terms: D000818:Animals; D002908:Chronic Disease; D005260:Female; D005508:Food Deprivation / Q000502:physiology*; D008297:Male; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism / Q000502:physiology*; D018482:Muscle, Skeletal / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013329:Structure-Activity Relationship
references.citation: Br J Nutr. 1982 May;47(3):433-7
references.pmid: 7082616
references.citation: Biochim Biophys Acta. 2005 Sep 25;1752(2):166-76
references.pmid: 16140047
references.citation: Res Exp Med (Berl). 1978 Jul 24;173(1):35-40
references.pmid: 150634
references.citation: J Anim Sci. 1994 Apr;72(4):911-7
references.pmid: 8014156
references.citation: J Appl Physiol (1985). 1993 Sep;75(3):1044-52
references.pmid: 8226510
references.citation: Am J Anat. 1984 Nov;171(3):259-72
references.pmid: 6517030
references.citation: Br J Nutr. 1982 May;47(3):417-31
references.pmid: 7082615
references.citation: Adv Exp Med Biol. 1987;225:287-304
references.pmid: 3331062
references.citation: Proteomics. 2001 Mar;1(3):424-34
references.pmid: 11680887
references.citation: J Exp Zool. 1968 Mar;167(3):353-8
references.pmid: 5659018
references.citation: J Cell Biol. 1968 Jul;38(1):1-14
references.pmid: 4300067
references.citation: Histochemistry. 1979 Feb 26;60(1):71-84
references.pmid: 34586
references.citation: Eur J Biochem. 1993 May 15;214(1):157-61
references.pmid: 8508787
references.citation: Ageing Res Rev. 2002 Feb;1(1):79-93
references.pmid: 12039450
references.citation: Clin Sci (Lond). 1984 Aug;67(2):185-94
references.pmid: 6744788
references.citation: Cell Tissue Res. 2005 Aug;321(2):233-41
references.pmid: 15947969
references.citation: Electrophoresis. 2000 Apr;21(6):1037-53
references.pmid: 10786879
references.citation: Exp Neurol. 1970 Aug;28(2):365-7
references.pmid: 4248172
references.citation: Nutr Neurosci. 2001;4(6):475-88
references.pmid: 11843267
references.citation: Br J Nutr. 2000 Aug;84(2):185-94
references.pmid: 11029969
references.citation: Eur J Histochem. 2007 Apr-Jun;51(2):111-8
references.pmid: 17664161
references.citation: J Proteomics. 2009 May 2;72(4):708-21
references.pmid: 19268720
references.citation: J Physiol. 2006 Feb 15;571(Pt 1):231-42
references.pmid: 16357018
references.citation: J Cell Comp Physiol. 1964 Apr;63:209-16
references.pmid: 14151089
references.citation: Anat Rec. 1974 Sep;180(1):53-61
references.pmid: 4137704
references.citation: Am J Physiol. 1991 Aug;261(2 Pt 1):C195-209
references.pmid: 1872366
references.citation: J Mol Cell Cardiol. 1995 Jan;27(1):121-31
references.pmid: 7760337
references.citation: Nature. 1971 Nov 12;234(5324):81-5
references.pmid: 4942892
references.citation: J Muscle Res Cell Motil. 2000;21(6):587-98
references.pmid: 11206136
references.citation: Mol Cell Biochem. 1998 Jul;184(1-2):169-82
references.pmid: 9746320
references.citation: Prog Biophys Mol Biol. 2000;73(2-4):195-262
references.pmid: 10958931
references.citation: J Anim Sci. 1974 May;38(5):1142-9
references.pmid: 4596892
references.citation: Proteomics. 2005 Jul;5(11):2896-906
references.pmid: 15981298
references.citation: Am J Clin Nutr. 1997 Aug;66(2):293-303
references.pmid: 9250107
references.citation: J Appl Physiol (1985). 1989 May;66(5):2196-205
references.pmid: 2745285
references.citation: Am J Physiol Regul Integr Comp Physiol. 2000 Apr;278(4):R845-54
references.pmid: 10749771
references.citation: Am J Physiol. 1991 Aug;261(2 Pt 1):L49-54
references.pmid: 1831330
references.citation: Am J Physiol. 1996 Mar;270(3 Pt 2):R667-74
references.pmid: 8780235
references.citation: Eur J Appl Physiol. 2000 Oct;83(2-3):166-74
references.pmid: 11104057
references.citation: J Physiol. 1979 Nov;296:453-69
references.pmid: 160929
references.citation: J Nutr. 1990 Nov;120 Suppl 11:1496-502
references.pmid: 2243295
references.citation: J Histochem Cytochem. 1969 Oct;17(10):675-80
references.pmid: 4194356
references.citation: J Anim Sci. 2005 Nov;83(11):2564-71
references.pmid: 16230653
references.citation: Exp Physiol. 1994 Jan;79(1):59-70
references.pmid: 8011317
references.citation: J Comp Neurol. 1994 Apr 22;342(4):596-602
references.pmid: 8040366
references.citation: J Biochem. 1993 Feb;113(2):200-7
references.pmid: 8468325
references.citation: J Physiol. 1979 Mar;288:285-300
references.pmid: 224167
references.citation: Annu Rev Biochem. 1985;54:831-62
references.pmid: 3896131
references.citation: Acta Physiol Scand. 1996 Mar;156(3):193-202
references.pmid: 8729679
references.citation: Acta Physiol Scand. 1998 Mar;162(3):229-45
references.pmid: 9578368
references.citation: Proteomics. 2004 Dec;4(12):3665-85
references.pmid: 15543535
references.citation: J Nutr. 2003 Jan;133(1):140-7
references.pmid: 12514281
references.citation: Cell Mol Life Sci. 2003 Feb;60(2):382-9
references.pmid: 12678501
references.citation: Can J Appl Physiol. 1998 Feb;23(1):1-22
references.pmid: 9494737
references.citation: J Appl Physiol (1985). 2004 Feb;96(2):645-9
references.pmid: 14715683
references.citation: J Nutr. 1964 Jan;82:10-8
references.pmid: 14110926
references.citation: J Physiol. 1973 Nov;234(3):723-48
references.pmid: 4148752
references.citation: J Anat. 1977 Feb;123(Pt 1):1-19
references.pmid: 190198