title: Diagnostic and prognostic value of plasma microRNA deregulation in nasopharyngeal carcinoma.
abstract: Nasopharyngeal carcinoma (NPC) is uncommon worldwide but often highly invasive in late stages. Due to its special location and lack of specific symptoms, NPC is hardly detected in regular medical examination at the beginning. Development of sensitive and specific biomarkers should help to save lives against this type of disease. In the present report, we investigated the value of plasma miRNAs for diagnosis and prognosis of NPC. Using candidate approach, we selected 21 miRNAs from literature to compare their expression levels in the plasma of NPC patients and controls. As a result, 5 miRNAs showed diagnostic potentials (P<0.01). Among them, miR-16, -21, -24, and -155 had increased levels in NPC patients, whereas the level of miR-378 was decreased. There was a negative correlation between plasma miRNA expression and cancer progression, where miR-21 was statistically significant in T and N staging and miR-16 and 24 were significant in N staging only. Combination of miR-16, -21, -24, -155, and -378 gives 87.7% of sensitivity and 82.0% of specificity for NPC diagnosis. Without miR-16, combination of the rest 4 miRNAs gives the same sensitivity but a slightly reduced specificity. After treatment, all 5 miRNAs were somewhat back to normal levels in patients without cancer recurrence but the prognostic value was not statistically significant. In conclusion, plasma miRNA expression is a useful biomarker for NPC diagnosis but not for its prognosis. More importantly, it is simple, effective, and non-invasive. Combination of several plasma miRNAs can increase both NPC diagnostic sensitivity and specificity.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery; Nanfang Hospital; Southern Medical University; Guangzhou, Guangdong PR China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery; Nanfang Hospital; Southern Medical University; Guangzhou, Guangdong PR China.
authors.forename: Hua-Nan
authors.initials: HN
authors.lastname: Luo
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery; Nanfang Hospital; Southern Medical University; Guangzhou, Guangdong PR China.
authors.forename: Wen-Dong
authors.initials: WD
authors.lastname: Tian
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery; Nanfang Hospital; Southern Medical University; Guangzhou, Guangdong PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery; Nanfang Hospital; Southern Medical University; Guangzhou, Guangdong PR China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery; Nanfang Hospital; Southern Medical University; Guangzhou, Guangdong PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Public Health and Tropical Medicine; Southern Medical University; Guangzhou, Guangdong PR China.
authors.forename: Bao
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery; Nanfang Hospital; Southern Medical University; Guangzhou, Guangdong PR China.
authors.forename: Bi-Jun
authors.initials: BJ
authors.lastname: Liang
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery; Nanfang Hospital; Southern Medical University; Guangzhou, Guangdong PR China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Peng
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery; Nanfang Hospital; Southern Medical University; Guangzhou, Guangdong PR China.
authors.forename: Shao-Xiong
authors.initials: SX
authors.lastname: Lin
authors.affiliation: Department of Neurology; The Sun Yat-Sen Memorial Hospital; Sun Yat-Sen University; Guangzhou, Guangdong PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery; Nanfang Hospital; Southern Medical University; Guangzhou, Guangdong PR China.
authors.forename: Xiang-Ping
authors.initials: XP
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D002277:Carcinoma; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis* / Q000473:pathology; D011379:Prognosis; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: NPC; biomarker; diagnosis; miRNA; nasopharyngeal carcinoma; plasma; prognosis
references.citation: Commun Integr Biol. 2010 Sep;3(5):478-81
references.pmid: 21057646
references.citation: RNA Biol. 2012 Jun;9(6):850-9
references.pmid: 22699556
references.citation: Ai Zheng. 2009 Oct;28(10):1029-32
references.pmid: 19799809
references.citation: Br J Haematol. 2008 May;141(5):672-5
references.pmid: 18318758
references.citation: Cancer Cell. 2004 May;5(5):423-8
references.pmid: 15144950
references.citation: Br J Cancer. 2009 Mar 24;100(6):1002-11
references.pmid: 19293812
references.citation: Lancet Oncol. 2012 Jun;13(6):633-41
references.pmid: 22560814
references.citation: Oncol Rep. 2011 May;25(5):1353-63
references.pmid: 21373758
references.citation: Ai Zheng. 2009 Oct;28(10):1022-8
references.pmid: 19799808
references.citation: J Virol. 2009 Mar;83(5):2357-67
references.pmid: 19091858
references.citation: J Clin Oncol. 2011 Dec 20;29(36):4781-8
references.pmid: 22105822
references.citation: J Exp Clin Cancer Res. 2012 Apr 30;31:38
references.pmid: 22546315
references.citation: Cancer Sci. 2010 Oct;101(10):2087-92
references.pmid: 20624164
references.citation: Nat Immunol. 2008 Sep;9(9):1065-73
references.pmid: 18677316
references.citation: BMC Public Health. 2008 Dec 05;8:400
references.pmid: 19055849
references.citation: Biochim Biophys Acta. 2012 Jan;1825(1):1-10
references.pmid: 21958739
references.citation: Exp Hematol. 2010 Dec;38(12):1126-30
references.pmid: 20977925
references.citation: Cytometry A. 2010 Jun;77(6):502-14
references.pmid: 20235276
references.citation: Nat Rev Cancer. 2011 Nov 24;11(12):849-64
references.pmid: 22113163
references.citation: Pathology. 2007 Apr;39(2):197-207
references.pmid: 17454749
references.citation: Nat Cell Biol. 2007 Jun;9(6):654-9
references.pmid: 17486113
references.citation: Drug Resist Updat. 2010 Jun;13(3):57-66
references.pmid: 20236855
references.citation: Nature. 2004 Sep 16;431(7006):350-5
references.pmid: 15372042
references.citation: Trends Microbiol. 2004 Aug;12(8):356-60
references.pmid: 15276610
references.citation: Cell Res. 2008 Oct;18(10):997-1006
references.pmid: 18766170
references.citation: Yonsei Med J. 2010 Sep;51(5):622-32
references.pmid: 20635434
references.citation: Oral Oncol. 2010 Mar;46(3):204-8
references.pmid: 20138800
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: Cancer. 2012 Feb 1;118(3):698-710
references.pmid: 21720996
references.citation: Int J Cancer. 2010 Dec 15;127(12):2870-8
references.pmid: 21351266
references.citation: J Cell Mol Med. 2009 Jan;13(1):39-53
references.pmid: 19175699
references.citation: Curr Drug Targets. 2010 Aug;11(8):926-35
references.pmid: 20415649
references.citation: Cell. 2004 Jan 23;116(2):281-97
references.pmid: 14744438
references.citation: Vet Rec. 1992 Mar 14;130(11):232
references.pmid: 1566538
references.citation: PLoS One. 2008;3(11):e3694
references.pmid: 19002258
references.citation: Proc Natl Acad Sci U S A. 2007 Jul 3;104(27):11400-5
references.pmid: 17600087
references.citation: Science. 2005 Dec 16;310(5755):1817-21
references.pmid: 16308420
references.citation: Nucleic Acids Res. 2011 Jan;39(Database issue):D152-7
references.pmid: 21037258