title: ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swine.
abstract: Haemophilus parasuis infection, known as Glässer's disease, is characterized by fibrinous polyserositis, arthritis and meningitis in piglets. Although traditional diagnosis is based on herd history, clinical signs, bacterial isolation and serotyping, the molecular-based methods are alternatives for species-specific tests and epidemiologic study. The aim of this study was to characterize H. parasuis strains isolated from different states of Brazil by serotyping, PCR and ERIC-PCR. Serotyping revealed serovar 4 as the most prevalent (24 %), followed by serovars 14 (14 %), 5 (12 %), 13 (8 %) and 2 (2 %), whereas 40 % of the strains were considered as non-typeable. From 50 strains tested 43 (86%) were positive to Group 1 vtaA gene that have been related to virulent strains of H.parasuis. ERIC-PCR was able to type isolates tested among 23 different patterns, including non-typeable strains. ERIC-PCR patterns were very heterogeneous and presented high similarity between strains of the same animal or farm origin. The results indicated ERIC-PCR as a valuable tool for typing H. parasuis isolates collected in Brazil.
authors.affiliation: Laboratório de Sanidade Suína e Virologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo , Sao Paulo, SP , Brasil.
authors.forename: Luisa Zanolli
authors.initials: LZ
authors.lastname: Moreno
authors.forename: Karina Salvagni
authors.initials: KS
authors.lastname: Castilla
authors.forename: Débora Dirani Sena
authors.initials: DD
authors.lastname: de Gobbi
authors.forename: Tania Alen
authors.initials: TA
authors.lastname: Coutinho
authors.forename: Thais Sebastiana Porfida
authors.initials: TS
authors.lastname: Ferreira
authors.forename: Andrea Micke
authors.initials: AM
authors.lastname: Moreno
keywords: ERIC-PCR; Genotyping; Glässer disease; Haemophilus parasuis
references.citation: Can Vet J. 1993 Aug;34(8):487-90
references.pmid: 17424269
references.citation: Am J Vet Res. 2003 Apr;64(4):435-42
references.pmid: 12693533
references.citation: Nucleic Acids Res. 1991 Dec 25;19(24):6823-31
references.pmid: 1762913
references.citation: Vet Microbiol. 1997 May;56(1-2):125-34
references.pmid: 9228688
references.citation: J Med Microbiol. 1969 Feb;2(1):75-8
references.pmid: 5821848
references.citation: J Clin Microbiol. 1990 Mar;28(3):495-503
references.pmid: 1691208
references.citation: Can J Vet Res. 1990 Apr;54 Suppl:S16-21
references.pmid: 2141799
references.citation: J Vet Diagn Invest. 2001 Nov;13(6):495-501
references.pmid: 11724140
references.citation: Vet J. 2012 Feb;191(2):213-8
references.pmid: 21247786
references.citation: Vet Microbiol. 2004 Mar 26;99(1):1-12
references.pmid: 15019107
references.citation: J Clin Microbiol. 1992 Apr;30(4):862-5
references.pmid: 1572971
references.citation: Vet J. 2011 Jun;188(3):362-4
references.pmid: 20580292
references.citation: Appl Environ Microbiol. 2006 Jun;72(6):3984-92
references.pmid: 16751506
references.citation: Microbiology (Reading). 2006 Dec;152(Pt 12):3683-3690
references.pmid: 17159221
references.citation: Aust Vet J. 2000 Dec;78(12):846-9
references.pmid: 11194474
references.citation: J Clin Microbiol. 1986 Jan;23(1):138-42
references.pmid: 3700597
references.citation: J Clin Microbiol. 1988 Nov;26(11):2465-6
references.pmid: 3069867
references.citation: Res Vet Sci. 2006 Feb;80(1):55-61
references.pmid: 15936788
references.citation: Antimicrob Agents Chemother. 2007 Jun;51(6):2260-4
references.pmid: 17438055
references.citation: Vet Microbiol. 2003 Apr 2;92(3):253-62
references.pmid: 12523987