title: Sensitive and visible detection of apoptotic cells on Annexin-V modified substrate using aminophenylboronic acid modified gold nanoparticles (APBA-GNPs) labeling.
abstract: A new strategy for sensitive detection of early stage apoptosis was proposed based on silver-enhanced gold nanoparticle (GNP) label method. Annexin-V modified substrate was constructed via layer-by-layer (LBL) method for specific capture of early stage apoptotic Jurkat cells. A new kind of aminophenylboronic acid modified gold nanoparticle (APBA-GNP) was synthesized and utilized for labeling cells, followed by silver enhancement. Anodic stripping voltammetry (ASV) was applied to sensitive detection of Ag(+) dissolved from the deposited silver particles, which reflected the number of cells. A good linear range from 1 × 10(2) to 3.5 × 10(3) cells was achieved, with a detection limit of 38 apoptotic cells. Moreover, the gray color of silver enhancement could be observed by the naked eye, which could be used to tell apoptotic cells apart from normal cells. Therefore, using the silver-enhanced GNP label method, apoptotic cells could not only be sensitively detected via electrochemical technique, but also can be discriminated from normal cells by the naked eye.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China.
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Pan
authors.forename: Wenqian
authors.initials: W
authors.lastname: Shan
authors.forename: Heting
authors.initials: H
authors.lastname: Fang
authors.forename: Manli
authors.initials: M
authors.lastname: Guo
authors.forename: Zhou
authors.initials: Z
authors.lastname: Nie
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D017304:Annexin A5 / Q000737:chemistry / Q000302:isolation & purification*; D017209:Apoptosis; D015374:Biosensing Techniques / Q000379:methods*; D001897:Boronic Acids / Q000737:chemistry*; D004563:Electrochemistry; D006046:Gold; D006801:Humans; D019169:Jurkat Cells; D053768:Metal Nanoparticles / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D017304:Annexin A5; D001897:Boronic Acids; C028592:3-aminobenzeneboronic acid; D012834:Silver; D006046:Gold
keywords: Aminophenylboronic acid (APBA); Annexin-V; Anodic stripping voltammetry (ASV); Apoptosis detection; Gold nanoparticles (GNPs); Silver enhancement