title: Isolation of the first IMP-4 metallo-β-lactamase producing Klebsiella pneumoniae in Tianjin, China.
abstract: This study shows for the first time the mechanism of carbapenem resistance of a Klebsiella pneumoniae clinical isolate TJ8 recovered from Tianjin Medical University General Hospital ,China. The modified Hodge test and EDTA synergy test were performed for the screening of carbapenemases and metallo-β-lactamases (MBLs), respectively. Polymerase chain reactions and DNA sequencing confirmed that the strain carried IMP-4 metallo-β-lactamase (MBL) , SHV-11 and TEM-1 β-lactamase. Class I integron was positive and gave a 3.0-kb PCR amplicon .IMP-4 was located in Class I integron 5'CS. The gene determinants were organized in the order of bla IMP-4-orfII-orfIII.In all, the results show that IMP-4 MBL production caused the TJ8 resistance to carbapenems.
authors.affiliation: Department of Clinical Laboratory, Tianjin Medical University General Hospital , Tianjin China, 300052.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Hu
authors.forename: Qiaojuan
authors.initials: Q
authors.lastname: Hu
keywords: Klebsiella pneumoniae; integron; metallo-β-lactamases
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