title: DNA repair gene polymorphisms and risk of head and neck cancer in the Tunisian population.
abstract: Altered activity of DNA repair enzymes may be involved in modulating cancer susceptibility and pathogenesis of head and neck cancer (HNC). We conducted a case-control study to test the association between three common single-nucleotide polymorphisms of XRCC1, ERCC2, and ERCC3 genes with HNC risk in Tunisian patients. To the best of our knowle dge, this is the first report on polymorphisms in XRCC1, ERCC2, and ERCC3 and susceptibility to HNC in our population. The genotype analyses of XRCC1 Arg399Gln, ERCC2 Lys751Gln, and ERCC3 7122 A>G polymorphisms for 169 HNC patients, and 261 controls were performed using the PCR-based restriction fragment length polymorphism. Stratification of the populations according to smoking and drinking habits and occupational exposure highlighted the importance of tobacco, alcohol, and toxic substance as three risk co-factors for the development of HNC. Our study suggests that only the XRCC1 Arg399Gln polymorphism was associated with the risk of HNC in the Tunisian population (OR = 2.04; P = 0.001). Furthermore, the risk of HNC was associated with XRCC1 Arg399Gln polymorphism stratified by occupational exposure status (OR = 2.29; P = 0.024). However, no statistically significant association was observed between the risk of developing HNC and the ERCC2 Lys751Gln and ERCC3 A>G polymorphisms. These data suggest that the XRCC1 Arg399Gln polymorphism is associated with an increased risk of developing HNC, because it correlates with occupational exposure in Tunisian population.
authors.affiliation: Marine Ecotoxicology, UR 09-03, Sfax University, IPEIS, Sfax, Tunisia; Bioinformatics Unit, Centre of Biotechnology of Sfax, Sfax, Tunisia.
authors.forename: Rim
authors.initials: R
authors.lastname: Khlifi
authors.forename: Imen
authors.initials: I
authors.lastname: Kallel
authors.forename: Bouthaina
authors.initials: B
authors.lastname: Hammami
authors.forename: Amel
authors.initials: A
authors.lastname: Hamza-Chaffai
authors.forename: Ahmed
authors.initials: A
authors.lastname: Rebai
mesh_terms: D000225:Adenine; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000428:Alcohol Drinking; D001120:Arginine / Q000235:genetics; D016022:Case-Control Studies; D004265:DNA Helicases / Q000235:genetics; D004260:DNA Repair / Q000235:genetics; D045643:DNA Repair Enzymes / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D059647:Gene-Environment Interaction; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005973:Glutamine / Q000235:genetics; D006147:Guanine; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006801:Humans; D008239:Lysine / Q000235:genetics; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors; D012907:Smoking; D000076105:X-ray Repair Cross Complementing Protein 1; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics; D055815:Young Adult
chemical_list: D004268:DNA-Binding Proteins; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human; D005973:Glutamine; C072022:XPBC-ERCC-3 protein; D006147:Guanine; D001120:Arginine; D004265:DNA Helicases; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human; D045643:DNA Repair Enzymes; D000225:Adenine; D008239:Lysine
keywords: ERCC2 and ERCC3 genes; XRCC1; head and neck cancer; occupational exposure; polymorphisms