title: Comparison of standard laminectomy with an optimized ejection method for the removal of spinal cords from rats and mice.
abstract: For researchers seeking to collect spinal cord samples from mice and rats while avoiding acid decalcification, few options are available. Laminectomy is the standard method which yields high quality samples, yet is time consuming and technically difficult. Ejection of the cord from the vertebral column is another technique commonly used; however, the literature suggests that this method can damage the spinal tissues and is typically avoided when histology of samples is the desired endpoint. Here, we describe an optimized method for ejection of spinal cords from rats and mice, and compare histological quality of these samples with those collected via laminectomy. Our results show that ejection can yield high quality spinal cord samples and may be suitable for use as an alternative to laminectomy.
authors.affiliation: Genentech, Inc., 1 DNA Way, MS 462a, South San Francisco, CA, California, USA.
authors.forename: Heather S
authors.initials: HS
authors.lastname: Kennedy
authors.forename: Charles
authors.initials: C
authors.lastname: Jones
authors.forename: Patrick
authors.initials: P
authors.lastname: Caplazi
keywords: Ejection; Histotechnology; Laminectomy; Mouse; Rat; Rodent; Rodent necropsy; Spinal cord
references.citation: PLoS One. 2011;6(7):e22537
references.pmid: 21799890
references.citation: J Neurosci Methods. 2010 Mar 15;187(1):59-66
references.pmid: 20043948
references.citation: Lab Anim (NY). 2011 Feb;40(2):53-7
references.pmid: 21252981
references.citation: Stain Technol. 1981 Jul;56(4):235-7
references.pmid: 7029784
references.citation: Brain Res Bull. 1981 May;6(5):449-50
references.pmid: 7248810