title: Understanding urinary conditioning film components on ureteral stents: profiling protein components and evaluating their role in bacterial colonization.
abstract: Ureteral stents are fraught with problems. A conditioning film attaches to the stent surface within hours of implantation; however, differences between stent types and their role in promoting encrustation and bacterial adhesion and colonization remain to be elucidated. The present work shows that the most common components do not differ between stent types or patients with the same indwelling stent, and contain components that may drive stent encrustation. Furthermore, unlike what was previously thought, the presence of a conditioning film does not increase bacterial adhesion and colonization of stents by uropathogens. Genitourinary cytokeratins are implicated in playing a significant role in conditioning film formation. Overall, stent biomaterial design to date has been unsuccessful in discovering an ideal coating to prevent encrustation and bacterial adhesion. This current study elucidates a more global understanding of urinary conditioning film components. It also supports specific focus on the importance of physical characteristics of the stent and how they can prevent encrustation and bacterial adhesion.
authors.affiliation: a Department of Urologic Sciences , The Stone Centre at Vancouver General Hospital, University of British Columbia , Vancouver , BC , Canada .
authors.forename: Chelsea N
authors.initials: CN
authors.lastname: Elwood
authors.forename: Joey
authors.initials: J
authors.lastname: Lo
authors.forename: Emily
authors.initials: E
authors.lastname: Chou
authors.forename: Adam
authors.initials: A
authors.lastname: Crowe
authors.forename: Olga
authors.initials: O
authors.lastname: Arsovska
authors.forename: Hans
authors.initials: H
authors.lastname: Adomat
authors.forename: Ricardo
authors.initials: R
authors.lastname: Miyaoka
authors.forename: Emma
authors.initials: E
authors.lastname: Tomlinson-Guns
authors.forename: Manoj
authors.initials: M
authors.lastname: Monga
authors.forename: Ben H
authors.initials: BH
authors.lastname: Chew
authors.forename: Dirk
authors.initials: D
authors.lastname: Lange
mesh_terms: D000328:Adult; D000368:Aged; D001422:Bacterial Adhesion; D001672:Biocompatible Materials / Q000032:analysis*; D018441:Biofilms / Q000254:growth & development*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D013058:Mass Spectrometry; D008875:Middle Aged; D015607:Stents / Q000145:classification; D014513:Ureter / Q000382:microbiology
chemical_list: D001672:Biocompatible Materials