title: Reversible expression of CD138 on mature follicular B cells is downregulated by IL-4.
abstract: CD138, known as a marker of plasma cells, was reported to be expressed to an intermediate level in the murine bone marrow precursor B cells. Here an intermediate level of CD138 expression was also noted in a subpopulation of splenic follicular B cells, which were distinguishable from CD138(high) plasma cells, whereas the majority of transitional or marginal zone B cells did not express CD138. These CD138(int) B cells were IgM(low)IgD(high) mature B cells, located within follicular B cell zone, and expressed a lower level of CD21 than CD138(-) follicular B cells. During in vitro culture of splenic cells, the proportion of CD138(int) B cells increased, which was noticeably reversed by the addition of IL-4 to the culture. The experiments with sorted CD138(int) cells showed that IL-4-mediated regulation of the CD138 expression was B cell-intrinsic and independent of in vitro B cell death. Our results demonstrate that mouse CD138(int) B cells characterize a subpopulation of IgM(low)IgD(high) mature follicular B cells. The CD138 expression on follicular B cells may represent a reversible status, reflecting a dynamic state probably influenced by IL-4.
authors.affiliation: Department of Molecular Cell Biology and Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea. Electronic address: jaeghi@skku.edu.
authors.forename: Jae-Ghi
authors.initials: JG
authors.lastname: Lee
authors.forename: Hana
authors.initials: H
authors.lastname: Moon
authors.forename: Chanho
authors.initials: C
authors.lastname: Park
authors.forename: Sang Hyuck
authors.initials: SH
authors.lastname: Shin
authors.forename: KyeongJin
authors.initials: K
authors.lastname: Kang
authors.forename: Tae Jin
authors.initials: TJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D018941:Antigens, CD19 / Q000276:immunology / Q000378:metabolism; D055610:Antigens, CD1d / Q000276:immunology / Q000378:metabolism; D016175:B-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018956:CD5 Antigens / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects / Q000276:immunology*; D005434:Flow Cytometry; D007072:Immunoglobulin D / Q000276:immunology / Q000378:metabolism; D007075:Immunoglobulin M / Q000276:immunology / Q000378:metabolism; D015847:Interleukin-4 / Q000276:immunology* / Q000494:pharmacology; D051917:Leukosialin / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D010950:Plasma Cells / Q000276:immunology / Q000378:metabolism; D017464:Receptors, Complement 3d / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D053668:Syndecan-1 / Q000276:immunology* / Q000378:metabolism
chemical_list: D018941:Antigens, CD19; D055610:Antigens, CD1d; D018956:CD5 Antigens; D007072:Immunoglobulin D; D007075:Immunoglobulin M; D051917:Leukosialin; D017464:Receptors, Complement 3d; C508242:Sdc1 protein, mouse; D053668:Syndecan-1; D015847:Interleukin-4
keywords: CD138; FO B cells; Follicular B cell; IL-4; MZ B cells; NKT cells; PNA; STAT6; Syndecan-1; T helper type 2 cells; Th2 cells; follicular B cells; interleukin-4; marginal zone B cells; natural killer T cells; peanut agglutinin; signal transducer and activator of transcription 6