title: Functional characterization of a juvenile hormone esterase related gene in the moth Sesamia nonagrioides through RNA interference.
abstract: Juvenile hormone esterase (JHE) is a carboxylesterase that has attracted great interest because of its critical role in regulating larval to adult transition in insects and other arthropods. Previously, we characterized an ecdysteroid sensitive and juvenile hormone non-susceptible juvenile hormone esterase related gene (SnJHER) in the corn stalk borer, Sesamia nonagrioides. SnJHER was rhythmically up-regulated close to each molt during the corn stalk borer's larval development. In this paper we attempted to functionally characterize SnJHER using several reverse genetics techniques. To functionally characterize SnJHER, we experimented with different dsRNA administration methods, including hemolymph, bacterial or baculovirus-mediated RNA interference, (RNAi). Our findings indicate the potential implication of SnJHER in the developmental programming of Sesamia nonagrioides. It is still unclear whether SnJHER is closely related to the authentic JHE gene, with different or similar biological functions.
authors.affiliation: Laboratory of Molecular Biology, Department of Agricultural Biotechnology, Agricultural University of Athens, Athens, Greece.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Kontogiannatos
authors.forename: Luc
authors.initials: L
authors.lastname: Swevers
authors.forename: Katsumi
authors.initials: K
authors.lastname: Maenaka
authors.forename: Enoch Y
authors.initials: EY
authors.lastname: Park
authors.forename: Kostas
authors.initials: K
authors.lastname: Iatrou
authors.forename: Anna
authors.initials: A
authors.lastname: Kourti
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D002265:Carboxylic Ester Hydrolases / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006458:Hemolymph / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009036:Moths / Q000235:genetics* / Q000254:growth & development; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D059386:Reverse Genetics / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D019476:Insect Proteins; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins; D002265:Carboxylic Ester Hydrolases; C038003:juvenile hormone esterase
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