title: Effects of commercial citrate-containing juices on urolithiasis in a Drosophila model.
abstract: Diet modification plays an important role in nephrolithiasis. Development of an easy, ready-to-use beverage such as a commercial juice drink to use as a preventive treatment for renal calculi formation would be widely welcomed. We previously developed a novel Drosophila model for the study of nephrolithiasis. It provides a new well-established drug discovery platform for this common disease. In our current study, we used the Drosophila model to investigate the preventive effects of various commercial juices as potential treatments for nephrolithiasis. Our results showed that apple, cranberry, orange, and pomegranate juices failed to reduce calcium oxalate (CaOx) crystal formation, whereas our positive control-potassium citrate (K-citrate)-significantly prevented CaOx crystal formation. Unlike the commercial fruit juices that were tested, the administration of K-citrate significantly ameliorated the ethylene glycol (EG)-induced life-span reduction in treated flies. These results indicate that EG-induced CaOx nephrolithiasis in Drosophila can be prevented by K-citrate, but not by commercial citrate-containing juices. However, the inhibitory capability of citrate-containing juices to reduce renal stone formation in humans requires further elucidation.
authors.affiliation: Institute of Chinese Medicine, Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung, Taiwan; Department of Family Medicine, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Chien-Yi
authors.initials: CY
authors.lastname: Ho
authors.forename: Yung-Hsiang
authors.initials: YH
authors.lastname: Chen
authors.forename: Pei-Yin
authors.initials: PY
authors.lastname: Wu
authors.forename: Chiao-Hui
authors.initials: CH
authors.lastname: Chang
authors.forename: Huey-Yi
authors.initials: HY
authors.lastname: Chen
authors.forename: Kee-Ming
authors.initials: KM
authors.lastname: Man
authors.forename: Jui-Lung
authors.initials: JL
authors.lastname: Shen
authors.forename: Fuu-Jen
authors.initials: FJ
authors.lastname: Tsai
authors.forename: Wei-Yong
authors.initials: WY
authors.lastname: Lin
authors.forename: Yuan-Ju
authors.initials: YJ
authors.lastname: Lee
authors.forename: Wen-Chi
authors.initials: WC
authors.lastname: Chen
mesh_terms: D000818:Animals; D002951:Citrates / Q000008:administration & dosage / Q000009:adverse effects*; D004195:Disease Models, Animal; D004330:Drosophila; D052878:Urolithiasis / Q000139:chemically induced*
chemical_list: D002951:Citrates
keywords: Calcium oxalate; Citrate; Drosophila; Juice; Urolithiasis
references.citation: N Engl J Med. 2002 Jan 10;346(2):77-84
references.pmid: 11784873
references.citation: Kidney Int. 2011 Aug;80(4):369-77
references.pmid: 21451462
references.citation: J Urol. 2007 Apr;177(4):1358-62; discussion 1362; quiz 1591
references.pmid: 17382731
references.citation: Am J Physiol Renal Physiol. 2010 Dec;299(6):F1237-44
references.pmid: 20926630
references.citation: Kidney Int. 2011 Aug;80(4):327-9
references.pmid: 21799502
references.citation: BJU Int. 2011 Feb;107(3):356-68
references.pmid: 21244607
references.citation: J Urol. 1993 Jun;149(6):1405-8
references.pmid: 8501777
references.citation: Ann Intern Med. 1997 Apr 1;126(7):497-504
references.pmid: 9092314
references.citation: World J Urol. 1997;15(4):236-43
references.pmid: 9280052
references.citation: Rev Urol. 2009 Summer;11(3):134-44
references.pmid: 19918339
references.citation: Urol Int. 2007;79 Suppl 1:26-31
references.pmid: 17726349
references.citation: Urol Res. 2008 Dec;36(6):313-7
references.pmid: 18946667
references.citation: J Urol. 1996 Sep;156(3):907-9
references.pmid: 8709360
references.citation: Eur Urol. 1990;17(3):248-51
references.pmid: 2190842
references.citation: Clin J Am Soc Nephrol. 2006 Nov;1(6):1269-74
references.pmid: 17699358
references.citation: BMC Urol. 2007 Oct 05;7:18
references.pmid: 17919315
references.citation: Curr Opin Urol. 2009 Mar;19(2):200-4
references.pmid: 19188774
references.citation: Urology. 2007 Nov;70(5):856-60
references.pmid: 17919696
references.citation: World J Urol. 1997;15(3):155-64
references.pmid: 9228722
references.citation: J Endourol. 2008 Mar;22(3):567-70
references.pmid: 18290732
references.citation: Urol Res. 1998;26(1):57-63
references.pmid: 9537698
references.citation: Ther Adv Urol. 2010 Feb;2(1):3-9
references.pmid: 21789078
references.citation: Ren Fail. 2009;31(6):522-31
references.pmid: 19839830
references.citation: Lancet. 2006 Jan 28;367(9507):333-44
references.pmid: 16443041
references.citation: Urol Int. 2009;82(4):464-71
references.pmid: 19506417
references.citation: Br J Nutr. 2003 Aug;90(2):295-300
references.pmid: 12908889
references.citation: Urology. 2008 Mar;71(3):379-83; discussion 383-4
references.pmid: 18342167
references.citation: Int Urol Nephrol. 2008;40(3):589-94
references.pmid: 17721827
references.citation: J Urol. 2005 Jan;173(1):271-5
references.pmid: 15592095
references.citation: J Formos Med Assoc. 2010 Dec;109(12):862-78
references.pmid: 21195884
references.citation: Gen Comp Endocrinol. 2007 Aug-Sep;153(1-3):3-12
references.pmid: 17324417
references.citation: J Urol. 2010 Sep;184(3):1189-96
references.pmid: 20663521
references.citation: Urol Res. 2008 Feb;36(1):17-24
references.pmid: 18040675
references.citation: Prim Care. 2010 Sep;37(3):565-81, ix
references.pmid: 20705199
references.citation: Eur J Clin Nutr. 2002 Oct;56(10):1020-3
references.pmid: 12373623
references.citation: J Urol. 2001 Jul;166(1):263-7
references.pmid: 11435883
references.citation: J Agric Food Chem. 2012 Mar 14;60(10):2753-7
references.pmid: 22352299
references.citation: J Urol. 2008 Feb;179(2):424-30
references.pmid: 18076928
references.citation: Urology. 2007 Jun;69(6):1013-6
references.pmid: 17572176
references.citation: Dis Model Mech. 2008 Jul-Aug;1(1):43-9
references.pmid: 19048052