title: Antitumor enhancement by adoptive transfer of tumor antigen primed, inactivated MHC-haploidentical lymphocytes.
abstract: The present study investigated the antitumor effects by adoptive transfer of tumor antigen primed, inactivated MHC-haploidentical lymphocytes in TC-1 lung cancer mouse model. Our studies revealed that the inactivated MHC-haploidentical effecter cells display the antitumor activity in vitro and target the tumor in vivo. After adoptive transferring these effecter cells, the Th1 cytokines such as IL-2 and IFN-γ are elevated in the serum; the recipient tumor-specific cytotoxic T-cells and natural killer cells are activated; tumor specific memory T cells are induced; tumor growth is inhibited and mouse survival is prolonged. The results indicate that MHC-haploidentical lymphocytes provide both effecter cells which can target the tumor cells through the identical MHC molecules and an adjuvant effects through the unmatched allogeneic MHC molecules which induces endogenous innate and adaptive antitumor immune responses.
authors.affiliation: Department of Immunology, Cancer Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China.
authors.forename: Guilan
authors.initials: G
authors.lastname: Shi
authors.affiliation: Department of Immunology, Cancer Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Department of Immunology, Cancer Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Immunology, Cancer Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Ma
authors.affiliation: Department of Immunology, Cancer Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China.
authors.forename: Binlei
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Immunology, Cancer Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China. Electronic address: zhangsr@cicams.ac.cn.
authors.forename: Shuren
authors.initials: S
authors.lastname: Zhang
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D007371:Interferon-gamma / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D008175:Lung Neoplasms / Q000276:immunology* / Q000628:therapy*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology*; D008285:Major Histocompatibility Complex; D008546:Melanoma, Experimental; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D000951:Antigens, Neoplasm; D007376:Interleukin-2; D007371:Interferon-gamma
keywords: Alloantigen; CTL; Cytotoxic T lymphocyte; FACS; FCS; GVHD; GVT; HVGR; Immunotherapy; MDSCs; MHC; MHC-haploidentical lymphocyte; MLC; MLTC; MMC; NIR; NK; PBS; SCT; TBI; Treg; Tumor; WBI; cytotoxic T lymphocyte; fetal calf serum; fluorescence-activated cell sorting; graft-versus-host disease; graft-versus-tumor; host-versus-graft response; mAbs; major histocompatibility complex; mitomycin C; mixed lymphocyte culture; mixed lymphocyte tumor culture; monoclonal antibodies; myeloid-derived suppressor cells; natural killer; near-infrared optical imaging; phosphate-buffered saline solution; regulatory T lympnocyte; stem cell transplantation; total body irradiation; whole-body imaging