title: The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism.
abstract: Cylindrospermopsis raciborskii (Woloszynska) Seenaya & Subba Raju is a freshwater cyanobacterium of worldwide distribution. In the North-eastern region of Brazil many eutrophic water reservoirs are characterized by the dominance of C. raciborskii, with recurrent occurrence of blooms. These water bodies have high conductivity due to a high ionic concentration, and are defined as hard (with high values of CaCO3). In this study, we investigated the long-term effect (12 days) of high calcium concentration (8 mM Ca(2+)) on C. raciborskii (T3 strain) growth, morphology, toxin content, and metabolism. Changes in protein expression profiles were investigated by proteomic analysis using 2D gel electrophoresis and mass spectrometry. A continued exposure to calcium had a pronounced effect on C. raciborskii (T3): it limited growth, decreased thricome length, increased chlorophyll-a content, altered toxin profile (although did not affect PST content, saxitoxin + neosaxitoxin), and inhibited the expression of proteins related to primary metabolism.
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro , Rio de Janeiro, RJ , Brasil ; Departamento de Análises Clínicas e Toxicológicas, Universidade de São Paulo , São Paulo, SP , Brasil.
authors.forename: Ronaldo Leal
authors.initials: RL
authors.lastname: Carneiro
authors.forename: Ana Carla Nascimento
authors.initials: AC
authors.lastname: Alípio
authors.forename: Paulo Mascarello
authors.initials: PM
authors.lastname: Bisch
authors.forename: Sandra Maria Feliciano
authors.initials: SM
authors.lastname: de Oliveira Azevedo
authors.forename: Ana Beatriz Furlanetto
authors.initials: AB
authors.lastname: Pacheco
keywords: Cylindrospermopsis; calcium; paralitic shellfish toxin; proteome
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