title: Opioid system genes in alcoholism: a case-control study in Croatian population.
abstract: Due to their involvement in dependence pathways, opioid system genes represent strong candidates for association studies investigating alcoholism. In this study, single nucleotide polymorphisms within the genes for mu (OPRM1) and kappa (OPRK1) opioid receptors and precursors of their ligands - proopiomelanocortin (POMC), coding for beta-endorphin and prodynorphin (PDYN) coding for dynorphins, were analyzed in a case-control study that included 354 male alcohol-dependent and 357 male control subjects from Croatian population. Analysis of allele and genotype frequencies of the selected polymorphisms of the genes OPRM1/POMC and OPRK1/PDYN revealed no differences between the tested groups. The same was true when alcohol-dependent persons were subdivided according to the Cloninger's criteria into type-1 and type-2 groups, known to differ in the extent of genetic control. Thus, the data obtained suggest no association of the selected polymorphisms of the genes OPRM1/POMC and OPRK1/PDYN with alcoholism in Croatian population.
authors.affiliation: Laboratory for Experimental Haematology, Immunology and Oncology, Division of Molecular Medicine, Rudjer Boskovic Institute, Bijenicka c. 54, HR-10000 Zagreb, Croatia. Electronic address: Barbara.Cupic@irb.hr.
authors.forename: B
authors.initials: B
authors.lastname: Cupic
authors.forename: J
authors.initials: J
authors.lastname: Stefulj
authors.forename: E
authors.initials: E
authors.lastname: Zapletal
authors.forename: A
authors.initials: A
authors.lastname: Matosic
authors.forename: T
authors.initials: T
authors.lastname: Bordukalo-Niksic
authors.forename: L
authors.initials: L
authors.lastname: Cicin-Sain
authors.forename: J
authors.initials: J
authors.lastname: Gabrilovac
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000437:Alcoholism / Q000235:genetics*; D016022:Case-Control Studies; D017523:Croatia; D004745:Enkephalins / Q000235:genetics*; D056726:Genetic Association Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011333:Pro-Opiomelanocortin / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D017473:Receptors, Opioid, kappa / Q000235:genetics*; D017450:Receptors, Opioid, mu / Q000235:genetics*; D055815:Young Adult
chemical_list: D004745:Enkephalins; C515653:OPRK1 protein, human; C485764:OPRM1 protein, human; D011498:Protein Precursors; D017473:Receptors, Opioid, kappa; D017450:Receptors, Opioid, mu; D011333:Pro-Opiomelanocortin; C033660:preproenkephalin
keywords: Alcohol dependence; Cloninger typology; OPRK1; OPRM1; PDYN; POMC; Single nucleotide polymorphism