title: [The role of SHH pathway in PDGF-induced VSMC proliferation].
abstract: OBJECTIVE
  To discuss the role of sonic hedgehog (SHH) pathway in PDGF-induced vascular smooth muscle cell proliferation.
  
  
  METHODS
  Human vein VSMC were cultured in vitro. Laser confocal microscopy and Western blot were used to detect the expression of SHH pathway related proteins. The cell proliferation was evaluated by Ki-67 staining and BrdU incorporation after treatment by siRNA of Gli2 or shh pathway inhibitor cyclopamine.
  
  
  RESULTS
  The results of laser confocal microscopy and Western blot showed that SHH pathway protein which including shh, Patched1 and Gli2 were activated in the PDGF-induced VSMC proliferation.BrdU incorporation assay and Ki-67 staining showed that the cell proliferation which induced by PDGF was inhibited by Gli2-siRNA and cyclopamine which can both block SHH pathway.
  
  
  CONCLUSIONS
  SHH pathway play an important role in PDGF-induced VSMC proliferation.
authors.affiliation: Department of Vascular Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Feng-he
authors.initials: FH
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xue-hu
authors.initials: XH
authors.lastname: Wang
authors.forename: De
authors.initials: D
authors.lastname: Shi
authors.forename: Qi-ning
authors.initials: QN
authors.lastname: Fu
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D053823:Hedgehog Proteins / Q000378:metabolism*; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D015398:Signal Transduction
chemical_list: D053823:Hedgehog Proteins; D010982:Platelet-Derived Growth Factor; C509239:SHH protein, human