title: New Arabidopsis thaliana cytochrome c partners: a look into the elusive role of cytochrome c in programmed cell death in plants.
abstract: Programmed cell death is an event displayed by many different organisms along the evolutionary scale. In plants, programmed cell death is necessary for development and the hypersensitive response to stress or pathogenic infection. A common feature in programmed cell death across organisms is the translocation of cytochrome c from mitochondria to the cytosol. To better understand the role of cytochrome c in the onset of programmed cell death in plants, a proteomic approach was developed based on affinity chromatography and using Arabidopsis thaliana cytochrome c as bait. Using this approach, ten putative new cytochrome c partners were identified. Of these putative partners and as indicated by bimolecular fluorescence complementation, nine of them bind the heme protein in plant protoplasts and human cells as a heterologous system. The in vitro interaction between cytochrome c and such soluble cytochrome c-targets was further corroborated using surface plasmon resonance. Taken together, the results obtained in the study indicate that Arabidopsis thaliana cytochrome c interacts with several distinct proteins involved in protein folding, translational regulation, cell death, oxidative stress, DNA damage, energetic metabolism, and mRNA metabolism. Interestingly, some of these novel Arabidopsis thaliana cytochrome c-targets are closely related to those for Homo sapiens cytochrome c (Martínez-Fábregas et al., unpublished). These results indicate that the evolutionarily well-conserved cytosolic cytochrome c, appearing in organisms from plants to mammals, interacts with a wide range of targets on programmed cell death. The data have been deposited to the ProteomeXchange with identifier PXD000280.
authors.affiliation: Instituto de Bioquímica Vegetal y Fotosíntesis (IBVF), Centro de Investigaciones Científicas Isla de la Cartuja (cicCartuja), Universidad de Sevilla-Consejo Superior de Investigaciones Científicas (CSIC), Seville, 41092, Spain;
authors.forename: Jonathan
authors.initials: J
authors.lastname: Martínez-Fábregas
authors.forename: Irene
authors.initials: I
authors.lastname: Díaz-Moreno
authors.forename: Katiuska
authors.initials: K
authors.lastname: González-Arzola
authors.forename: Simon
authors.initials: S
authors.lastname: Janocha
authors.forename: José A
authors.initials: JA
authors.lastname: Navarro
authors.forename: Manuel
authors.initials: M
authors.lastname: Hervás
authors.forename: Rita
authors.initials: R
authors.lastname: Bernhardt
authors.forename: Antonio
authors.initials: A
authors.lastname: Díaz-Quintana
authors.forename: Miguel Á
authors.initials: MÁ
authors.lastname: De la Rosa
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002846:Chromatography, Affinity; D045304:Cytochromes c / Q000235:genetics / Q000378:metabolism*; D003600:Cytosol / Q000737:chemistry / Q000378:metabolism; D004734:Energy Metabolism; D019143:Evolution, Molecular; D018506:Gene Expression Regulation, Plant; D057809:HEK293 Cells; D006801:Humans; D013058:Mass Spectrometry; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism; D058977:Molecular Sequence Annotation; D018384:Oxidative Stress; D011485:Protein Binding; D025941:Protein Interaction Mapping; D021381:Protein Transport; D011523:Protoplasts / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D020349:Surface Plasmon Resonance
chemical_list: D029681:Arabidopsis Proteins; D012333:RNA, Messenger; D045304:Cytochromes c
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