title: [Effects of umbilical cord blood monocytes transplantation on EPO protein and oligodendrocyte progenitors in neonatal rats with hypoxic-ischemic brain damage].
abstract: OBJECTIVE
  To study the effects of umbilical cord blood monocytes (UCBMC) transplantation on erythropoietin (EPO) protein and oligodendrocyte progenitor cells in hypoxia-ischemia (HI) neonatal rats.
  
  
  METHODS
  Forty seven-day-old Sprague-Dawley rats were randomly divided into normal control (N), HI, UCBMC and HI+UCBMC groups (n=10 each). Hypoxic-ischemic brain damage (HIBD) model was prepared according to the Rice method. Twenty-four hours after hypoxia, the N and HI groups were injected with 2 μL phosphate buffered saline (PBS), and the UCBMC and HI+UCBMC groups were injected with 3×10(6) UCBMC via the lateral ventricle. EPO protein and oligodendrocyte progenitor cells in the subventricular zone of the injured brain were observed by EPO/DAPI and NG2/DAPI immunofluorescence double staining, and their correlation was analyzed.
  
  
  RESULTS
  Seven days after transplantation, there were more NG2(+)DAPI(+) and EPO(+)DAPI(+) cells in the HI+UCBMC group than in the UCBMC (P<0.05), N and HI groups (P<0.01). More NG2(+)DAPI(+) and EPO(+)DAPI(+) cells were observed in the UCBMC group compared with the N and HI groups (P<0.01). There were more NG2(+)DAPI(+) cells in the N group than in the HI group (P<0.01). The number of NG2(+)DAPI(+) cells was correlated with the number of EPO(+)DAPI(+) cells in the HI+UCBMC group (r=0.898, β=1.4604, P<0.01).
  
  
  CONCLUSIONS
  UCBMC can promote expression of oligodendrocyte progenitor cells, which is correlated with an increase in EPO protein and thus repairs brain white matter damage in neonatal rats with HIBD.
authors.affiliation: Department of Pediatrics, Clinical college, Weifang Medical University, Weifang, Shandong 261031, China. wxlpine@163.com.
authors.forename: Jia-Fen
authors.initials: JF
authors.lastname: Ji
authors.forename: Jin-Ping
authors.initials: JP
authors.lastname: Zhang
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Wang
authors.forename: Qing-Jie
authors.initials: QJ
authors.lastname: Mu
authors.forename: Meng-Meng
authors.initials: MM
authors.lastname: Fan
authors.forename: Yu-Xi
authors.initials: YX
authors.lastname: Chen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004921:Erythropoietin / Q000032:analysis / Q000096:biosynthesis*; D005312:Fetal Blood / Q000166:cytology*; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D009000:Monocytes / Q000637:transplantation*; D009836:Oligodendroglia / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000473:pathology*
chemical_list: D004921:Erythropoietin