title: Physiological studies on carboxymethyl cellulase formation by Aspergillus terreus DSM 826.
abstract: Physiological studies were conducted to determine the optimum cultural conditions for maximal carboxymethyl cellulase (CMCase) formation by Aspergillus terreus DSM 826. Shaking condition at 150 rpm is favorable for the production of CMCase from rice straw and sugar cane bagasse. The highest enzyme yield was obtained at the third day of incubation at 30 °C for both cases; however CMCase formation occurred at a broad range of pH values, with maximal formation of A. terreus DSM 826 CMCase at pH 4.5 and 5.0 when rice straw and sugar cane bagasse were used as sole carbon source, respectively. Carboxymethyl cellulose (CMC) was found to be a good inducer for CMCase formation in both agricultural wastes with CMC concentrations of 0.5 and 1.0 % (w/v) in case of rice straw and sugar cane bagasse, respectively. High level of enzyme formation was obtained with the addition of ammonium chloride as nitrogen source in both cases and at a concentration of 0.4 % (v/v Tween-80) as an addition to medium containing rice straw. However this addition did not influence the production of CMCase in case of using sugar cane bagasse as carbon source.
authors.affiliation: Department of Microbial Chemistry, National Research Centre , Dokki, Cairo , Egypt.
authors.forename: Osama M
authors.initials: OM
authors.lastname: Abdel-Fatah
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Hassan
authors.forename: Ali M
authors.initials: AM
authors.lastname: Elshafei
authors.forename: Bakry M
authors.initials: BM
authors.lastname: Haroun
authors.forename: Housam M
authors.initials: HM
authors.lastname: Atta
authors.forename: Abdelmageed M
authors.initials: AM
authors.lastname: Othman
keywords: Aspergillus terreus; carboxymethyl cellulase (CMCase); rice straw; sugar cane bagasse
references.citation: J Bacteriol. 1984 Dec;160(3):1047-54
references.pmid: 6094497
references.citation: Can J Microbiol. 1976 Aug;22(8):1153-9
references.pmid: 963627
references.citation: Biotechnol Bioeng. 1983 Oct;25(10):2395-8
references.pmid: 18548568
references.citation: Braz J Microbiol. 2008 Jan;39(1):122-7
references.pmid: 24031191
references.citation: Biosci Biotechnol Biochem. 1998 Sep;62(9):1751-6
references.pmid: 9805376
references.citation: Bioresour Technol. 2008 Jan;99(2):378-86
references.pmid: 17320379
references.citation: J Biol Chem. 1951 Nov;193(1):265-75
references.pmid: 14907713
references.citation: Biochemistry (Mosc). 2002 Aug;67(8):850-71
references.pmid: 12223085
references.citation: J Ind Microbiol Biotechnol. 2003 Jul;30(7):440-4
references.pmid: 12844245
references.citation: Bioresour Technol. 2007 May;98(7):1467-73
references.pmid: 17097876
references.citation: Biotechnol Bioeng. 1984 Apr;26(4):370-3
references.pmid: 18553304
references.citation: Appl Environ Microbiol. 1985 May;49(5):1314-22
references.pmid: 3923931