title: Proteomics approaches advance our understanding of plant self-incompatibility response.
abstract: Self-incompatibility (SI) in plants is a genetic mechanism that prevents self-fertilization and promotes out-crossing needed to maintain genetic diversity. SI has been classified into two broad categories: the gametophytic self-incompatibility (GSI) and the sporophytic self-incompatibility (SSI) based on the genetic mechanisms involved in 'self' pollen rejection. Recent proteomic approaches to identify potential candidates involved in SI have shed light onto a number of previously unidentified mechanisms required for SI response. SI proteome research has progressed from the use of isoelectric focusing in early days to the latest third-generation technique of comparative isobaric tag for relative and absolute quantitation (iTRAQ) used in recent times. We will focus on the proteome-based approaches used to study self-incompatibility (GSI and SSI), recent developments in the field of incompatibility research with emphasis on SSI and future prospects of using proteomic approaches to study self-incompatibility.
authors.affiliation: Department of Biological Sciences, University of Calgary , BI 392, 2500 University Drive Northwest, Calgary, Alberta T2N 1N4, Canada.
authors.forename: Subramanian
authors.initials: S
authors.lastname: Sankaranarayanan
authors.forename: Muhammad
authors.initials: M
authors.lastname: Jamshed
authors.forename: Marcus A
authors.initials: MA
authors.lastname: Samuel
mesh_terms: D002853:Chromatography, Liquid; D019295:Computational Biology / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional; D046228:Microarray Analysis; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000235:genetics / Q000502:physiology*; D040901:Proteomics / Q000379:methods*; D060436:Self-Incompatibility in Flowering Plants / Q000235:genetics*; D013045:Species Specificity; D053719:Tandem Mass Spectrometry
chemical_list: D010940:Plant Proteins