title: In vivo analgesic activity, toxicity and phytochemical screening of the hydroalcoholic extract from the leaves of Psidium cattleianum Sabine.
abstract: BACKGROUND
  Psidium cattleianum Sabine is extensively used in Brazilian traditional medicine to treat several diseases including painful disorders. Aim of the study to investigate the toxicity and the possible analgesic activities of the hydroalcoholic extract from the leaves of Psidium cattleianum Sabine (ELPCS), to support its use in folk medicine. To screen the major phytochemical constituents of this extract and evaluate their antioxidant activity.
  
  
  METHODS
  ELPCS was assessed for its antioxidant activity using the DPPH model. Its analgesic activity was examined using mouse models of acetic acid-induced writhing and hot plate paw licking models. The major phytochemical constituents of the extract were screened; their toxicity on LLC-MK2 mammalian cells was evaluated.
  
  
  RESULTS
  ELPCS exhibited significant peripheral analgesic activity at doses of 60, 80, 100, 200 and 400mg/kg in mice, but it did not display central analgesic activity and not was toxic to LLC-MK2 cell (LD50>400 µg/mL). The extract exhibited free radical scavenging activity as evidenced by IC50 values (15.9 µg/mL) obtained by the DPPH method. Phytochemical screening detected flavonoids, saponins, cardiac glycosides, anthraquinones, and tannins.
  
  
  CONCLUSIONS
  The results of the experimental studies proved the analgesic activity of ELPCS and supported the traditional use of this plant.
authors.affiliation: Universidade Estadual de Montes Claros, Campus Universitário Professor Darcy Ribeiro, Av Ruy Braga S/N, CEP 39401-089, Vila Mauriceia, Montes Claros-MG, Brazil.
authors.forename: Felipe Queiroz
authors.initials: FQ
authors.lastname: Alvarenga
authors.forename: Bárbara C F
authors.initials: BC
authors.lastname: Mota
authors.forename: Marcel N
authors.initials: MN
authors.lastname: Leite
authors.forename: Jaciara M S
authors.initials: JM
authors.lastname: Fonseca
authors.forename: Dario A
authors.initials: DA
authors.lastname: Oliveira
authors.forename: Vanessa de Andrade
authors.initials: Vde A
authors.lastname: Royo
authors.forename: Márcio L A
authors.initials: ML
authors.lastname: e Silva
authors.forename: Viviane
authors.initials: V
authors.lastname: Esperandim
authors.forename: Alexandre
authors.initials: A
authors.lastname: Borges
authors.forename: Rosangela S
authors.initials: RS
authors.lastname: Laurentiz
mesh_terms: D019342:Acetic Acid; D000700:Analgesics / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001713:Biphenyl Compounds / Q000378:metabolism; D002460:Cell Line; D000431:Ethanol / Q000737:chemistry; D006358:Hot Temperature; D008253:Macaca mulatta; D008297:Male; D051379:Mice; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy*; D008517:Phytotherapy; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves; D031647:Psidium; D012997:Solvents / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D000700:Analgesics; D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; D014867:Water; D000431:Ethanol; C004931:1,1-diphenyl-2-picrylhydrazyl; D019342:Acetic Acid
keywords: Analgesic activity; Myrtaceae; Phytochemistry; Psidium cattleianum Sabine; Toxicity