title: Elevated CD8 T cell responses in type 1 diabetes patients to a 13 amino acid coeliac-active peptide from α-gliadin.
abstract: Some type 1 diabetes (T1D) patients have been reported to exhibit T cell reactivity to wheat gluten. We tested the hypothesis that this T cell reactivity could be abolished by using prolyl-endopeptidase (PEP), an enzyme that cleaves peptide bonds after proline. Peripheral blood mononuclear cells (PBMCs) were isolated from T1D patients and healthy controls. PBMCs were stimulated with a peptic-tryptic digest of wheat gluten; a peptic-tryptic-PEP digest of wheat gluten; and a 13 amino acid peptide from wheat gluten. Fluorescent-labelled antibodies to CD3, CD4 and CD8 cell marker proteins were utilized to determine proliferative responses of CD3, CD4 and CD8 T cells. There were no significant differences in proliferative responses of CD3 or CD4 T cells to the wheat gluten antigens. A significantly higher proportion of CD8(+) T cells from T1D patients proliferated in the presence of the 13 amino acid peptide than when challenged with the peptic-tryptic or the peptic-tryptic-PEP digests of wheat gluten. PEP treatment had no significant effect on CD8 T cell reactivity to the peptic-trytic digest of wheat gluten. Our results suggest that wheat gluten-derived peptides, containing ≤ 13 amino acids, may evoke T cell responses in T1D patients.
authors.affiliation: Department of Human Nutrition, Foods and Exercise, Virginia Tech, Blacksburg, VA, USA.
authors.forename: W E
authors.initials: WE
authors.lastname: Barbeau
authors.forename: R
authors.initials: R
authors.lastname: Hontecillas
authors.forename: W
authors.initials: W
authors.lastname: Horne
authors.forename: A
authors.initials: A
authors.lastname: Carbo
authors.forename: M H
authors.initials: MH
authors.lastname: Koch
authors.forename: J
authors.initials: J
authors.lastname: Bassaganya-Riera
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D017252:CD3 Complex / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D010450:Endopeptidases / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D005903:Gliadin / Q000276:immunology*; D005983:Glutens / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000276:immunology* / Q000378:metabolism; D055815:Young Adult
chemical_list: D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D010455:Peptides; D005983:Glutens; D005903:Gliadin; D010450:Endopeptidases
keywords: T cell responses; peptides; prolyl-endopeptidase; type 1 diabetes; wheat gluten
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