title: Efficient utilization of aerobic metabolism helps Tibetan locusts conquer hypoxia.
abstract: BACKGROUND
  Responses to hypoxia have been investigated in many species; however, comparative studies between conspecific geographical populations at different altitudes are rare, especially for invertebrates. The migratory locust, Locusta migratoria, is widely distributed around the world, including on the high-altitude Tibetan Plateau (TP) and the low-altitude North China Plain (NP). TP locusts have inhabited Tibetan Plateau for over 34,000 years and thus probably have evolved superior capacity to cope with hypoxia.
  
  
  RESULTS
  Here we compared the hypoxic responses of TP and NP locusts from morphological, behavioral, and physiological perspectives. We found that TP locusts were more tolerant of extreme hypoxia than NP locusts. To evaluate why TP locusts respond to extreme hypoxia differently from NP locusts, we subjected them to extreme hypoxia and compared their transcriptional responses. We found that the aerobic metabolism was less affected in TP locusts than in NP locusts. RNAi disruption of PDHE1β, an entry gene from glycolysis to TCA cycle, increased the ratio of stupor in TP locusts and decreased the ATP content of TP locusts in hypoxia, confirming that aerobic metabolism is critical for TP locusts to maintain activity in hypoxia.
  
  
  CONCLUSIONS
  Our results indicate that TP and NP locusts have undergone divergence in hypoxia tolerance. These findings also indicate that insects can adapt to hypoxic pressure by modulating basic metabolic processes.
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. lkang@ioz.ac.cn.
authors.forename: Dejian
authors.initials: D
authors.lastname: Zhao
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Zhang
authors.forename: Arianne
authors.initials: A
authors.lastname: Cease
authors.forename: Jon
authors.initials: J
authors.lastname: Harrison
authors.forename: Le
authors.initials: L
authors.lastname: Kang
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D000531:Altitude; D000818:Animals; D002681:China; D019143:Evolution, Molecular; D000860:Hypoxia / Q000378:metabolism*; D048188:Locusta migratoria / Q000235:genetics / Q000378:metabolism*; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D010641:Phenotype; D018609:Tibet; D059467:Transcriptome
references.citation: Nucleic Acids Res. 2006 Jul 1;34(Web Server issue):W293-7
references.pmid: 16845012
references.citation: PLoS Genet. 2011 Feb 03;7(2):e1001291
references.pmid: 21304893
references.citation: Proc Natl Acad Sci U S A. 1991 May 1;88(9):3832-6
references.pmid: 1902572
references.citation: Proc Natl Acad Sci U S A. 1988 Oct;85(19):7336-40
references.pmid: 3140238
references.citation: Mol Cell Biol. 1997 Dec;17(12):6924-31
references.pmid: 9372924
references.citation: Nucleic Acids Res. 2006 Jul 1;34(Web Server issue):W720-4
references.pmid: 16845106
references.citation: Mol Biol Evol. 2011 Jan;28(1):351-63
references.pmid: 20685719
references.citation: PLoS One. 2009;4(1):e3876
references.pmid: 19127286
references.citation: J Exp Biol. 2009 Feb;212(Pt 3):424-8
references.pmid: 19151217
references.citation: J Insect Physiol. 2012 Nov;58(11):1424-31
references.pmid: 22985864
references.citation: Trends Ecol Evol. 2006 Jan;21(1):29-37
references.pmid: 16701467
references.citation: Symp Soc Exp Biol. 1988;42:181-201
references.pmid: 3077857
references.citation: Science. 1964 Jan 24;143(3604):369-71
references.pmid: 14074855
references.citation: Science. 2010 Jul 2;329(5987):75-8
references.pmid: 20595611
references.citation: Dev Growth Differ. 2006 Sep;48(7):439-45
references.pmid: 16961591
references.citation: BMC Syst Biol. 2009 Sep 09;3:91
references.pmid: 19740440
references.citation: Proc Natl Acad Sci U S A. 2011 Mar 8;108(10):3882-7
references.pmid: 21325054
references.citation: Int J Cancer. 2014 Jan 15;134(2):249-56
references.pmid: 23564219
references.citation: PLoS One. 2010 Mar 29;5(3):e9911
references.pmid: 20360971
references.citation: J Insect Physiol. 1997 Mar;43(3):203-210
references.pmid: 12769903
references.citation: Cell. 2007 Apr 6;129(1):111-22
references.pmid: 17418790
references.citation: Science. 2010 Jul 2;329(5987):72-5
references.pmid: 20466884
references.citation: Mol Syst Biol. 2007;3:99
references.pmid: 17437024
references.citation: Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17611-5
references.pmid: 15591108
references.citation: Hum Genet. 2004 Jul;115(2):123-7
references.pmid: 15138885
references.citation: Can J Microbiol. 1978 May;24(5):586-92
references.pmid: 657011
references.citation: Arch Pharm Res. 2013 Mar;36(3):252-63
references.pmid: 23440580
references.citation: Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10809-12
references.pmid: 9380715
references.citation: Proc Biol Sci. 2013 Feb 06;280(1756):20122758
references.pmid: 23390104
references.citation: PLoS Genet. 2007 Mar 30;3(3):e45
references.pmid: 17397259
references.citation: J Chromatogr. 1980 Jan 11;181(1):103-7
references.pmid: 7364902
references.citation: Mol Ecol. 2012 Sep;21(17):4344-58
references.pmid: 22738353
references.citation: PLoS One. 2007 May 30;2(5):e490
references.pmid: 17534440
references.citation: J Biol Chem. 2000 Aug 25;275(34):26458-66
references.pmid: 10843999
references.citation: Nat Commun. 2013;4:1858
references.pmid: 23673643
references.citation: J Nucleic Acids. 2010 Dec 16;2010:592980
references.pmid: 21209706
references.citation: Biochem J. 1952 Nov;52(3):527-8
references.pmid: 13018271
references.citation: Mol Biol Evol. 2011 Feb;28(2):1075-81
references.pmid: 21030426
references.citation: Epigenetics. 2010 May 16;5(4):293-6
references.pmid: 20418669
references.citation: FEBS Open Bio. 2012 Aug 13;2:235-40
references.pmid: 23650605
references.citation: BMC Evol Biol. 2009 Jun 28;9:144
references.pmid: 19558707
references.citation: J Biol Chem. 2003 Jun 6;278(23):21240-6
references.pmid: 12651851
references.citation: Biochem J. 1999 Dec 15;344 Pt 3:903-14
references.pmid: 10585880
references.citation: Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6780-4
references.pmid: 1713691
references.citation: Trends Genet. 2006 Aug;22(8):456-61
references.pmid: 16806568
references.citation: J Exp Biol. 2004 Jan;207(Pt 3):497-508
references.pmid: 14691097