title: Nutritional requirement among Pseudomonas aeruginosa isolates recovered from respiratory clinical specimens at a tertiary hospital from South of Brazil.
abstract: We screened 349 isolates of P. aeruginosa from cystic fibrosis (CF+) and non-cystic fibrosis (CF-) patients for the auxotrophy. Fourteen (4.0%) were auxotrophic and among them only one was recovered from CF-patient showing that this characteristic is strongly associated with cystic fibrosis. In total, a requirement for 5 different compounds (or combination) was verified and, of these, methionine was the most common single amino acid required. Only one auxotrophic isolate was no able to produce biofilm in vitro.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul , Porto Alegre, RS , Brasil.
authors.forename: Leandro Reus Rodrigues
authors.initials: LR
authors.lastname: Perez
authors.forename: Ana Lúcia Peixoto
authors.initials: AL
authors.lastname: de Freitas
authors.forename: Afonso Luís
authors.initials: AL
authors.lastname: Barth
keywords: Pseudomonas aeruginosa; auxotrophy; biofilm; cystic fibrosis
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