title: Expanding the library and substrate diversity of the pyrrolysyl-tRNA synthetase to incorporate unnatural amino acids containing conjugated rings.
abstract: Unnatural amino acids (UAAs) containing conjugated ring systems are of interest for their optical properties. Until now, such bulky and planar UAAs could not be incorporated into proteins using the pyrrolysyl tRNA/synthetase shuttling system. Using the "small-intelligent" approach to construct a highly diverse library, we evolved novel synthetases specific for two such UAAs and incorporated them into proteins in E. coli and mammalian cells.
authors.affiliation: Jack H. Skirball Center for Chemical Biology & Proteomics, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA 92037 (USA).
authors.forename: Vanessa K
authors.initials: VK
authors.lastname: Lacey
authors.forename: Gordon V
authors.initials: GV
authors.lastname: Louie
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Noel
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism*; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000818:Animals; D019020:Directed Molecular Evolution; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D005815:Genetic Code; D008239:Lysine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D009211:Myoglobin / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D014907:Whales / Q000235:genetics
chemical_list: D000596:Amino Acids; D009211:Myoglobin; D000604:Amino Acyl-tRNA Synthetases; C456839:pyrrolysine; D008239:Lysine
keywords: directed evolution; genetic code expansion; pyrrolysine; small-intelligent library; unnatural amino acids
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