title: Human (α2→6) and avian (α2→3) sialylated receptors of influenza A virus show distinct conformations and dynamics in solution.
abstract: Differential interactions between influenza A virus protein hemagglutinin (HA) and α2→3 (avian) or α2→6 (human) sialylated glycan receptors play an important role in governing host specificity and adaptation of the virus. Previous analysis of HA-glycan interactions with trisaccharides showed that, in addition to the terminal sialic acid linkage, the conformation and topology of the glycans, while they are bound to HA, are key factors in regulating these interactions. Here, the solution conformation and dynamics of two representative avian and human glycan pentasaccharide receptors [LSTa, Neu5Ac-α(2→3)-Gal-β(1→3)-GlcNAc-β(1→3)-Gal-β(1→4)-Glc; LSTc, (Neu5Ac-α(2→6)-Gal-β(1→4)-GlcNAc-β(1→3)-Gal-β(1→4)-Glc] have been explored using nuclear magnetic resonance and molecular dynamics simulation. Analyses demonstrate that, in solution, human and avian receptors sample distinct conformations, topologies, and dynamics. These unique features of avian and human receptors in solution could represent distinct molecular characteristics for recognition by HA, thereby providing the HA-glycan interaction specificity in influenza.
authors.affiliation: Istituto di Ricerche Chimiche e Biochimiche "G. Ronzoni", Via Giuseppe Colombo, 81, Milano, 20133 Italy.
authors.forename: Guilherme L
authors.initials: GL
authors.lastname: Sassaki
authors.affiliation: Istituto di Ricerche Chimiche e Biochimiche "G. Ronzoni", Via Giuseppe Colombo, 81, Milano, 20133 Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Elli
authors.affiliation: Istituto di Ricerche Chimiche e Biochimiche "G. Ronzoni", Via Giuseppe Colombo, 81, Milano, 20133 Italy.
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Rudd
authors.affiliation: Istituto di Ricerche Chimiche e Biochimiche "G. Ronzoni", Via Giuseppe Colombo, 81, Milano, 20133 Italy.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Macchi
authors.affiliation: Department of Structural and Chemical Biology, Biosciences Building, University of Liverpool, Crown Street, Liverpool L69 7ZB, U.K.
authors.forename: Edwin A
authors.initials: EA
authors.lastname: Yates
authors.affiliation: Istituto di Ricerche Chimiche e Biochimiche "G. Ronzoni", Via Giuseppe Colombo, 81, Milano, 20133 Italy.
authors.forename: Annamaria
authors.initials: A
authors.lastname: Naggi
authors.affiliation: Harvard-MIT Division of Health Sciences and Technology, Koch Institute for Integrative Cancer Research, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.
authors.forename: Zachary
authors.initials: Z
authors.lastname: Shriver
authors.affiliation: Harvard-MIT Division of Health Sciences and Technology, Koch Institute for Integrative Cancer Research, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.
authors.forename: Rahul
authors.initials: R
authors.lastname: Raman
authors.affiliation: Harvard-MIT Division of Health Sciences and Technology, Koch Institute for Integrative Cancer Research, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.
authors.forename: R
authors.initials: R
authors.lastname: Sasisekharan
authors.affiliation: Istituto di Ricerche Chimiche e Biochimiche "G. Ronzoni", Via Giuseppe Colombo, 81, Milano, 20133 Italy.
authors.forename: Giangiacomo
authors.initials: G
authors.lastname: Torri
authors.affiliation: Istituto di Ricerche Chimiche e Biochimiche "G. Ronzoni", Via Giuseppe Colombo, 81, Milano, 20133 Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Guerrini
mesh_terms: D000818:Animals; D001717:Birds; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000378:metabolism*; D005585:Influenza in Birds / Q000378:metabolism* / Q000821:virology; D007251:Influenza, Human / Q000378:metabolism* / Q000821:virology; D008958:Models, Molecular; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D011991:Receptors, Virus / Q000737:chemistry* / Q000378:metabolism
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011134:Polysaccharides; D011991:Receptors, Virus; D019158:N-Acetylneuraminic Acid
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