title: Multiple interactions of NaHER1 protein with abscisic acid signaling in Nicotiana attenuata plants.
abstract: Previously, we identified a novel herbivore elicitor-regulated protein in Nicotiana attenuata (NaHER1) that is required to suppress abscisic acid (ABA) catabolism during herbivore attack and activate a full defense response against herbivores. ABA, in addition to its newly defined role in defense activation, mainly controls seed germination and stomatal function of land plants. Here we show that N. attenuata seeds silenced in the expression of NaHER1 by RNA interference (irHER1) accumulated less ABA during germination, and germinated faster on ABA-containing media compared to WT. Curiously, epidermal cells of irHER1 plants were wrinkled, possibly due to the previously demonstrated increase in transpiration of irHER1 plants that may affect turgor and cause wrinkling of the cells. We conclude that NaHER1 is a highly pleiotropic regulator of ABA responses in N. attenuata plants.
authors.affiliation: Department of Molecular Ecology; Max Planck Institute for Chemical Ecology; Jena, Germany.
authors.forename: Son Truong
authors.initials: ST
authors.lastname: Dinh
authors.affiliation: Department of Molecular Ecology; Max Planck Institute for Chemical Ecology; Jena, Germany.
authors.forename: Ian T
authors.initials: IT
authors.lastname: Baldwin
authors.affiliation: Department of Molecular Ecology; Max Planck Institute for Chemical Ecology; Jena, Germany.
authors.forename: Ivan
authors.initials: I
authors.lastname: Gális
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D020868:Gene Silencing; D018525:Germination; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D011485:Protein Binding; D012639:Seeds / Q000254:growth & development / Q000378:metabolism; D015398:Signal Transduction; D014026:Nicotiana / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D000040:Abscisic Acid
keywords: ABA metabolism; Nicotiana attenuata; abscisic acid (ABA); germination
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