title: Phylogenetic analysis and genetic characterization of chicken anemia virus isolates from Cambodia.
abstract: Three chicken anemia viruses (CAV) were detected by PCR during screening of field samples from village chickens collected in Cambodia in 2011/2012. Nearly full-length VP1 viral structural protein genes (nt 1-1,293) from the 3 CAV were sequenced and characterized. Phylogenetic analysis revealed that all 3 of the Cambodian CAV were clustered with CAV strains belonging to genotype II and were most closely related to CAV strains from Guangdong province, China. On the amino acid level, major substitutions were observed at 12 residues in the VP1 protein (positions 22, 75, 97, 125, 139, 144, 254, 287, 290, 370, 376, and 413) when compared with published reference CAV strains. In motifs associated with virulence, all Cambodian CAV had virulence-associated motifs composed of 75I, 89T, 125I, 139Q, 141Q, 144Q, and 394Q, which are commonly found in highly virulent genotype II viruses and some genotype III viruses. This is the first report of CAV isolated from village chickens in Southeast Asia as well as Cambodia.
authors.affiliation: Avian Diseases Division, Animal and Plant Quarantine Agency, Gyeonggi 430-757, Republic of Korea; and.
authors.forename: Soo-Jeong
authors.initials: SJ
authors.lastname: Kye
authors.forename: Ji-Ye
authors.initials: JY
authors.lastname: Kim
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Seul
authors.forename: Seromi
authors.initials: S
authors.lastname: Kim
authors.forename: Sang-Eun
authors.initials: SE
authors.lastname: Kim
authors.forename: Hee-Soo
authors.initials: HS
authors.lastname: Lee
authors.forename: San
authors.initials: S
authors.lastname: Sorn
authors.forename: Kang-Seuk
authors.initials: KS
authors.lastname: Choi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002160:Cambodia; D017727:Chicken anemia virus / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002645:Chickens; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D015678:Viral Structural Proteins