title: Antagonistic activity expressed by Shigella sonnei: identification of a putative new bacteriocin.
abstract: Bacteriocins are antibacterial, proteinaceous substances that mediate microbial dynamics. Bacteriocin production is a highly disseminated property among all major lineages of bacteria, including Shigella. In this paper, we addressed the purification and characterisation of a bacteriocin produced by a Shigella sonnei strain (SS9) isolated from a child with acute diarrhoea. The substance was purified through ammonium-sulphate precipitation and sequential steps of chromatography. The intracellular fraction obtained at 75% ammonium sulphate maintained activity following exposure to pH values from 1-11 and storage at -80ºC for more than two years and was inactivated by high temperatures and proteases. The molecular mass of the purified bacteriocin was determined by mass spectrometry to be 18.56 kDa. The N-terminal sequence of the bacteriocin did not match any other antibacterial proteins described. A putative new bacteriocin produced by S. sonnei has been detected. This bacteriocin may represent a newly described protein or a previously described protein with a newly detected function. Considering that SS9 expresses antagonism against other diarrhoeagenic bacteria, the bacteriocin may contribute to S. sonnei virulence and is potentially applicable to either preventing or controlling diarrhoeal disease.
authors.affiliation: Departamento de Microbiologia, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo HorizonteMG, Brasil.
authors.forename: Mireille Ângela Bernardes
authors.initials: MÂ
authors.lastname: Sousa
authors.forename: Luiz de Macêdo
authors.initials: Lde M
authors.lastname: Farias
authors.forename: Patrícia Luciana de
authors.initials: PL
authors.lastname: Oliveira
authors.forename: Jaqueline Silvana
authors.initials: JS
authors.lastname: Moreira
authors.forename: Ana Carolina Morais
authors.initials: AC
authors.lastname: Apolônio
authors.forename: Jamil Silvano
authors.initials: JS
authors.lastname: Oliveira
authors.forename: Marcelo Matos
authors.initials: MM
authors.lastname: Santoro
authors.forename: Edilberto Nogueira
authors.initials: EN
authors.lastname: Mendes
authors.forename: Paula Prazeres
authors.initials: PP
authors.lastname: Magalhães
mesh_terms: D000208:Acute Disease; D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D056148:Chromatography, Reverse-Phase; D003967:Diarrhea / Q000382:microbiology; D006801:Humans; D013058:Mass Spectrometry; D012764:Shigella sonnei / Q000737:chemistry* / Q000254:growth & development
chemical_list: D001430:Bacteriocins
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