title: Investigation of the direct hepatic effects of intramuscular interleukin-8 injection in an experimental rabbit model.
abstract: BACKGROUND
  The aim of this study was to investigate the effects of intramuscular IL-8 injection on hepatic tissues using an in vivo histopathological animal model.
  
  
  METHODS
  Twelve New Zealand white rabbits were used for this randomized, controlled, single-blinded interventional study. For 6 days, 1 gluteus maximus muscle was injected daily with 1 mcg/kg of IL-8 in 6 rabbits (Group A). The remaining 6 rabbits (to determine to normal porto-hepatic morphology of the rabbit genus) were in the sham group (Group B). At the end of the 7th day, all rabbits were killed and livers were meticulously harvested. Microscopically, regional tissues were scored according to portal inflammation, focal necrosis, piecemeal necrosis, and total impact.
  
  
  RESULTS
  Total impact score, portal inflammation, focal necrosis, and piecemeal necrosis were the histopathologic changes present in a higher incidence in the IL-8 group compared with the control group. The differences were significant when the groups were compared according to total impact score, portal inflammation, focal necrosis, and piecemeal necrosis according to Pearson's correlation (p<0.05). The most significant differences were detected at the total impact scores (p=0.002) and the portal inflammation scores (p=0.008).
  
  
  CONCLUSIONS
  Our results showed that IL-8 may damage hepatocytes. This can be the determined target for new therapeutic strategies. Further trials should be designed to obtain definitive results.
authors.affiliation: Department of Cardiovascular Surgery, Katip Celebi University Faculty of Medicine, Izmir Ataturk Training and Research Hospital, Izmir, Turkey.
authors.forename: Mert
authors.initials: M
authors.lastname: Kestelli
authors.forename: Mehmet
authors.initials: M
authors.lastname: Guzeloglu
authors.forename: Ismail
authors.initials: I
authors.lastname: Yurekli
authors.forename: Habib
authors.initials: H
authors.lastname: Cakir
authors.forename: Yeliz
authors.initials: Y
authors.lastname: Yilmaz
authors.forename: Eda
authors.initials: E
authors.lastname: Erdis
authors.forename: Saliha
authors.initials: S
authors.lastname: Aksun
authors.forename: Engin
authors.initials: E
authors.lastname: Tulukoğlu
authors.forename: Ragip
authors.initials: R
authors.lastname: Ortac
mesh_terms: D000818:Animals; D006801:Humans; D007273:Injections, Intramuscular; D016209:Interleukin-8 / Q000008:administration & dosage* / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D023421:Models, Animal; D011817:Rabbits
chemical_list: D016209:Interleukin-8
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