title: Differential pathogenicity among Tomato leaf curl Gujarat virus isolates from India.
abstract: Tomato leaf curl Gujarat virus (ToLCGV) has been identified as one of the most destructive pathogens causing tomato leaf curl disease (ToLCD) in India. In the tomato growing regions of Dhanbad and Ramgarh, plants bearing severe symptoms of ToLCD such as leaf curling, leaf crinkling, yellowing and leaf rolling was observed in the farmer fields. The association of begomovirus in these samples was confirmed by PCR and the causal viruses were identified as the isolates of ToLCGV. However, association of cognate DNA B component could not be ascertained from these samples. Indeed, like other Old World begomoviruses, the present ToLCGV isolates were found to be associated with a particular betasatellite, Tomato yellow leaf curl Thailand betasatellite (TYLCTHB). Although DNA A of both ToLCGV isolates could alone infect tomato inducing systemic symptoms, the difference in virulence was observed. Co-inoculation of TYLCTHB reduced the incubation period without influencing the accumulation of helper virus DNA and hence, differential pathogenesis among ToLCGV isolates was governed by the helper component rather than betasatellite. ToLCGV infection with DNA B increases the accumulation of DNA A component of Dhanbad isolate but not of Ramgarh isolate. Results indicated that the begomovirus identified from Ramgarh sample was a mild strain of ToLCGV.
authors.affiliation: Molecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.
authors.forename: Punam
authors.initials: P
authors.lastname: Ranjan
authors.forename: R Vinoth
authors.initials: RV
authors.lastname: Kumar
authors.forename: S
authors.initials: S
authors.lastname: Chakraborty
mesh_terms: D000595:Amino Acid Sequence; D053839:Begomovirus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D007194:India; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D014774:Virulence
chemical_list: D014764:Viral Proteins
references.citation: Arch Virol. 2008;153(4):763-81
references.pmid: 18247103
references.citation: J Virol. 2007 Nov;81(21):11972-81
references.pmid: 17715241
references.citation: Anal Biochem. 1983 Jul 1;132(1):6-13
references.pmid: 6312838
references.citation: J Virol Methods. 2006 Jul;135(1):9-16
references.pmid: 16513183
references.citation: Mol Biotechnol. 2002 Mar;20(3):315-8
references.pmid: 11936260
references.citation: J Virol. 1999 Jul;73(7):5481-9
references.pmid: 10364296
references.citation: Mol Biol Evol. 2011 Oct;28(10):2731-9
references.pmid: 21546353
references.citation: J Gen Virol. 2008 Dec;89(Pt 12):3165-3172
references.pmid: 19008407
references.citation: Arch Virol. 2013 Dec;158(12):2633-9
references.pmid: 23836393
references.citation: Phytopathology. 2003 Dec;93(12):1485-95
references.pmid: 18943612
references.citation: Phytopathology. 2005 Aug;95(8):902-8
references.pmid: 18944412
references.citation: Arch Virol. 2008;153(4):783-821
references.pmid: 18256781
references.citation: Arch Virol. 2005 May;150(5):845-67
references.pmid: 15703846
references.citation: Arch Virol. 2011 May;156(5):769-83
references.pmid: 21311922
references.citation: Virology. 2004 Jun 20;324(1):37-47
references.pmid: 15183051
references.citation: PLoS Pathog. 2011 Oct;7(10):e1002329
references.pmid: 22028660
references.citation: Virology. 2000 Nov 25;277(2):429-38
references.pmid: 11080490
references.citation: Virology. 1999 Oct 10;263(1):1-14
references.pmid: 10544077
references.citation: FEBS Lett. 2009 Jun 18;583(12):1825-32
references.pmid: 19497325
references.citation: Virol J. 2007 Oct 26;4:111
references.pmid: 17963488
references.citation: Virology. 1991 Jan;180(1):416-9
references.pmid: 1984661
references.citation: Virus Genes. 2012 Feb;44(1):112-9
references.pmid: 21969121
references.citation: J Virol. 2005 Aug;79(16):10764-75
references.pmid: 16051868