title: FIZZ1 could enhance the angiogenic ability of rat aortic endothelial cells.
abstract: Found in inflammatory zone (FIZZ1), also known as hypoxia-induced mitogenic factor (HIMF), is a secreted protein formed by 111 amino acid residues. FIZZ1 is mainly located in alveolar epithelial cells, white adipose tissue and the heart. This study aimed to explore the effects of FIZZ1 on the angiogenic ability of cultured rat aortic endothelial cells (RAECs) and the potential mechanism. The RAECs were cultured in the extracellular matrix (ECM) supplemented with 10% fetal bovine serum (FBS). Matrigel assay was used to detect the angiogenic ability of the RAECs and Agilent Rat Microarray containing 41,000 genes/ESTs was used to screen the differentially expressed genes of the RAECs after they were treated with FIZZ1 (5 x 10(-9)~2 x 10(-8) mol/L). The results were verified using RT-PCR method. We found that FIZZ1 markedly enhanced the angiogenic ability of RAECs (22.6 ± 2.94 vs. 19.7 ± 2.57, P < 0.01; 28.5 ± 3.32 vs. 19.7 ± 2.57, P < 0.01; 36.9 ± 5.01 vs. 19.7 ± 2.57, P < 0.01) in a dose-dependent manner (5 x 10(-9)~2 x 10(-8) mol/L). 440 genes (Gng8, Atg9a, Gdf6, etc.) were found to be up-regulated and 497 genes (Hbb-b1, Camk1g, etc.) down-regulated in the experimental group. Changes in Gng8 and Atg9a were revealed by RT-PCR. FIZZ1 could enhance angiogenesis of RAECs by up-regulating Gng8 and Atg9a.
authors.affiliation: Department of Cardiology, The General Hospital of Jinan Military Region Jinan 250031, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.forename: Yongyao
authors.initials: Y
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Fang
authors.forename: Hongming
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D008297:Male; D018919:Neovascularization, Physiologic; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; C486575:Retnla protein, rat; D020932:Nerve Growth Factor
keywords: FIZZ1/RELMα; RAECs; angiogenesis; gene chip; gene expression profiling
references.citation: Curr Opin Cell Biol. 2010 Apr;22(2):124-31
references.pmid: 20034776
references.citation: J Biol Chem. 1995 Mar 24;270(12):6757-67
references.pmid: 7896821
references.citation: Am J Physiol Lung Cell Mol Physiol. 2006 Dec;291(6):L1159-68
references.pmid: 16891392
references.citation: J Asthma. 2008 Oct;45(8):648-53
references.pmid: 18951255
references.citation: J Immunol. 2007 Dec 1;179(11):7800-7
references.pmid: 18025226
references.citation: J Immunol. 2010 Jul 15;185(2):1205-14
references.pmid: 20543100
references.citation: Am J Respir Cell Mol Biol. 2012 May;46(5):703-13
references.pmid: 22246861
references.citation: PLoS Pathog. 2009 Apr;5(4):e1000393
references.pmid: 19381262
references.citation: EMBO J. 2000 Aug 1;19(15):4046-55
references.pmid: 10921885
references.citation: J Immunol. 2009 Feb 15;182(4):2357-63
references.pmid: 19201890
references.citation: J Exp Med. 2009 Dec 21;206(13):2947-57
references.pmid: 19995957
references.citation: Respir Res. 2006 Mar 02;7:37
references.pmid: 16512910
references.citation: J Exp Med. 2009 Apr 13;206(4):937-52
references.pmid: 19349464
references.citation: Respir Res. 2012 Jun 22;13:51
references.pmid: 22726462
references.citation: Lung. 2008 Nov-Dec;186(6):375-80
references.pmid: 18758859
references.citation: J Pathol. 2007 Jun;212(2):180-7
references.pmid: 17492827
references.citation: Am J Pathol. 2009 May;174(5):1745-55
references.pmid: 19349363
references.citation: Am J Respir Cell Mol Biol. 2006 Oct;35(4):444-56
references.pmid: 16709959
references.citation: J Leukoc Biol. 2002 Apr;71(4):597-602
references.pmid: 11927645
references.citation: Angiogenesis. 2010 Jun;13(2):71-4
references.pmid: 20559867
references.citation: Am J Pathol. 2004 Apr;164(4):1315-26
references.pmid: 15039219
references.citation: Blood. 1998 May 1;91(9):3430-8
references.pmid: 9558402
references.citation: J Immunol. 2010 Nov 1;185(9):5539-48
references.pmid: 20889544
references.citation: J Biol Chem. 2003 Aug 15;278(33):31000-6
references.pmid: 12788940
references.citation: Proc Natl Acad Sci U S A. 2001 Jan 16;98(2):502-6
references.pmid: 11209052
references.citation: Circ Res. 2003 May 30;92(10):1065-7
references.pmid: 12714564
references.citation: Circulation. 2002 Mar 5;105(9):1135-43
references.pmid: 11877368