title: The design of MACs (minimal actin cortices).
abstract: The actin cell cortex in eukaryotic cells is a key player in controlling and maintaining the shape of cells, and in driving major shape changes such as in cytokinesis. It is thereby constantly being remodeled. Cell shape changes require forces acting on membranes that are generated by the interplay of membrane coupled actin filaments and assemblies of myosin motors. Little is known about how their interaction regulates actin cell cortex remodeling and cell shape changes. Because of the vital importance of actin, myosin motors and the cell membrane, selective in vivo experiments and manipulations are often difficult to perform or not feasible. Thus, the intelligent design of minimal in vitro systems for actin-myosin-membrane interactions could pave a way for investigating actin cell cortex mechanics in a detailed and quantitative manner. Here, we present and discuss the design of several bottom-up in vitro systems accomplishing the coupling of actin filaments to artificial membranes, where key parameters such as actin densities and membrane properties can be varied in a controlled manner. Insights gained from these in vitro systems may help to uncover fundamental principles of how exactly actin-myosin-membrane interactions govern actin cortex remodeling and membrane properties for cell shape changes.
authors.affiliation: Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Martinsried, Germany.
authors.forename: Sven K
authors.initials: SK
authors.lastname: Vogel
authors.forename: Fabian
authors.initials: F
authors.lastname: Heinemann
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Chwastek
authors.forename: Petra
authors.initials: P
authors.lastname: Schwille
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000199:Actins / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D003584:Cytological Techniques / Q000379:methods*; D008051:Lipid Bilayers / Q000378:metabolism; D009218:Myosins / Q000378:metabolism; D011817:Rabbits
chemical_list: D000199:Actins; D008051:Lipid Bilayers; D000255:Adenosine Triphosphate; D009218:Myosins
keywords: actin; actin cortex; actomyosin; membrane; myosin
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