title: Using Drosophila larvae to study epidermal wound closure and inflammation.
abstract: This methods chapter describes two methods for creating epithelial wounds in Drosophila larvae: pinch and puncture wounding. It also covers protocols for visualizing epithelial wounds, either in a dissected whole mount preparation or, using transgenic reporter larvae, in a live whole mount preparation. Finally, useful transgenic lines for live genetic screening of genes required for wound closure or inflammation are described.
authors.affiliation: Department of Biochemistry and Molecular Biology, The University of Texas Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: Sirisha
authors.initials: S
authors.lastname: Burra
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Amanda R
authors.initials: AR
authors.lastname: Brock
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Galko
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004330:Drosophila; D004817:Epidermis / Q000293:injuries*; D007150:Immunohistochemistry / Q000379:methods; D007249:Inflammation / Q000209:etiology* / Q000473:pathology*; D007814:Larva; D008853:Microscopy; D014945:Wound Healing
references.citation: J Cell Biol. 2005 Feb 14;168(4):567-73
references.pmid: 15699212
references.citation: Nature. 2007 Jul 12;448(7150):151-6
references.pmid: 17625558
references.citation: Proc Natl Acad Sci U S A. 2011 Aug 30;108(35):14560-5
references.pmid: 21841138
references.citation: Curr Biol. 2009 Sep 15;19(17):1473-7
references.pmid: 19646875
references.citation: Dev Cell. 2003 Nov;5(5):673-90
references.pmid: 14602069
references.citation: J Vis Exp. 2009 Feb 04;(24):
references.pmid: 19229190
references.citation: Dev Biol. 2002 Jan 1;241(1):145-56
references.pmid: 11784101
references.citation: Mol Biotechnol. 2004 Oct;28(2):147-66
references.pmid: 15477654
references.citation: J Cell Sci. 2007 Apr 1;120(Pt 7):1209-15
references.pmid: 17356067
references.citation: Nat Rev Genet. 2005 Jan;6(1):9-23
references.pmid: 15630418
references.citation: Immunol Lett. 2005 Oct 15;101(1):108-11
references.pmid: 15964636
references.citation: Nat Cell Biol. 2002 Nov;4(11):907-12
references.pmid: 12402048
references.citation: Dev Biol. 2005 Apr 1;280(1):73-86
references.pmid: 15766749
references.citation: Genetics. 2010 Nov;186(3):943-57
references.pmid: 20813879
references.citation: Proc Natl Acad Sci U S A. 2008 Jul 22;105(29):10017-22
references.pmid: 18632567
references.citation: Genesis. 2002 Sep-Oct;34(1-2):1-15
references.pmid: 12324939
references.citation: PLoS Biol. 2004 Aug;2(8):E239
references.pmid: 15269788