title: Echinococcus granulosus in Portugal: the first report of the G7 genotype in cattle.
abstract: Although cystic echinococcosis (CE) has been a recognized public health problem in Portugal, molecular data regarding the types and prevalence of infecting strains of its etiological agent (Echinococcus granulosus) are still scarce. Over the last years we have been evaluating the prevalence of CE in the country, and in this report we determined the parasite genotypes infecting sheep, goats, cattle and human in Portugal, based on 209 hydatid cysts recovered from liver (n=96), lung (n=95), pancreas (n=17) and kidney (n=1) samples obtained between 2008 and 2011. Protoscoleces or germinal layers were collected from individual cysts, DNA was extracted, and a part of the mitochondrial DNA encoding the cytochrome c oxidase subunit 1 was amplified by PCR. Overall, the results confirm the overall dominance of the G1-G3 cluster of strains, which are particularly prevalent in southern Portugal in livestock ruminants. Unexpectedly, one parasite sequence with cattle origin was found to correspond to E. granulosus G7 genotype (also known as E. intermedius), here reported for the first time in bovine, in Portugal.
authors.affiliation: Escola Superior de Saúde Dr. Lopes Dias, Instituto Politécnico de Castelo Branco, Campus da Talagueira, 6000-767 Castelo Branco, Portugal; Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa (UNL), Unidade de Parasitologia e Microbiologia Médicas (UPMM), Rua da Junqueira 100, 1349-008 Lisboa, Portugal. Electronic address: silviabeato@ipcb.pt.
authors.forename: Sílvia
authors.initials: S
authors.lastname: Beato
authors.forename: Ricardo
authors.initials: R
authors.lastname: Parreira
authors.forename: Cláudio
authors.initials: C
authors.lastname: Roque
authors.forename: Matilde
authors.initials: M
authors.lastname: Gonçalves
authors.forename: Liliana
authors.initials: L
authors.lastname: Silva
authors.forename: Maria Paola
authors.initials: MP
authors.lastname: Maurelli
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Cringoli
authors.forename: Maria Amélia
authors.initials: MA
authors.lastname: Grácio
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology*; D018509:DNA, Helminth / Q000235:genetics; D004443:Echinococcosis / Q000453:epidemiology / Q000662:veterinary*; D004444:Echinococcosis, Hepatic / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D048209:Echinococcus granulosus / Q000235:genetics*; D005838:Genotype; D015511:Goat Diseases / Q000453:epidemiology / Q000469:parasitology; D006041:Goats; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011174:Portugal / Q000453:epidemiology; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000469:parasitology
chemical_list: D018509:DNA, Helminth
keywords: Echinococcus granulosus; Genotypes; Intermediate hosts; Mitochondrial cytochrome oxidase; Portugal