title: Osmo-, Thermo- and Ethanol- Tolerances of Saccharomyces cerevisiae S1.
abstract: Saccharomyces cerevisiae S1, which is a locally isolated and improved strain showed viability at 40, 45 and 50°C and produced ethanol at 40, 43 and 45°C. When the cells were given heat shock at 45°C for 30min and grown at 40°C, 100% viability was observed for 60h, and addition of 200gL(-1) ethanol has led to complete cell death at 30h. Heat shock given at 45°C (for 30min) has improved the tolerance to temperature induced ethanol shock leading to 37% viability at 30h. When the cells were subjected to ethanol (200gL(-1) for 30 min) and osmotic shock (sorbitol 300gL(-1)), trehalose contents in the cells were increased. The heat shocked cells showed better viability in presence of added ethanol. Soy flour supplementation has improved the viability of S. cerevisiae S1 to 80% in presence of 100gL(-1) added ethanol and to 60% in presence of 300gL(-1)sorbitol. In presence of sorbitol (200gL(-1)) and ethanol (50gL(-1)) at 40°C, 46% viability was retained by S. cerevisiae S1 at 48h and it was improved to 80% by soy flour supplementation.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, University of Jaffna , Kokuvil , Sri Lanka.
authors.forename: Sandrasegarampillai
authors.initials: S
authors.lastname: Balakumar
authors.forename: Vasanthy
authors.initials: V
authors.lastname: Arasaratnam
keywords: Saccharomyces cerevisiae; Thermo-tolerance; ethanol-tolerance; osmo-tolerance; viability
references.citation: FEBS Lett. 1986 Oct 20;207(1):149-52
references.pmid: 3533625
references.citation: Philos Trans R Soc Lond B Biol Sci. 1993 Mar 29;339(1289):279-85; discussion 285-6
references.pmid: 8098532
references.citation: Biochim Biophys Acta. 1994 May 11;1191(2):309-16
references.pmid: 8172916
references.citation: Yeast. 1987 Dec;3(4):223-32
references.pmid: 3332975
references.citation: Antonie Van Leeuwenhoek. 1999 Apr;75(3):245-51
references.pmid: 10427413
references.citation: Plant Cell. 1994 Dec;6(12):1889-97
references.pmid: 7866031
references.citation: J Appl Microbiol. 2008 Mar;104(3):767-75
references.pmid: 17927743
references.citation: FEMS Microbiol Lett. 1997 Jul 1;152(1):11-5
references.pmid: 9228764
references.citation: Trends Biotechnol. 1998 Nov;16(11):460-8
references.pmid: 9830154
references.citation: Arch Microbiol. 1980 Feb;124(2-3):285-7
references.pmid: 6989337
references.citation: Biotechnol Bioeng. 2005 Nov 20;92(4):403-9
references.pmid: 16028292
references.citation: FEMS Microbiol Rev. 1993 Aug;11(4):339-55
references.pmid: 8398211
references.citation: Science. 1984 Feb 17;223(4637):701-3
references.pmid: 17841031
references.citation: Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11091-4
references.pmid: 1763024
references.citation: Biotechnol Bioeng. 1999 Oct 20;65(2):176-81
references.pmid: 10458738
references.citation: FEMS Yeast Res. 2004 Jan;4(4-5):351-9
references.pmid: 14734015
references.citation: Biochem Biophys Res Commun. 1980 Apr 14;93(3):819-24
references.pmid: 6992776
references.citation: FEMS Microbiol Lett. 1997 Jan 1;146(1):65-71
references.pmid: 8997708
references.citation: FEBS Lett. 1987 Aug 10;220(1):113-5
references.pmid: 3301407
references.citation: FEBS Lett. 1990 Oct 29;273(1-2):107-10
references.pmid: 2146164
references.citation: Mol Microbiol. 1997 Aug;25(3):571-81
references.pmid: 9302019
references.citation: FEBS Lett. 1991 Aug 19;288(1-2):86-90
references.pmid: 1831771
references.citation: Mol Microbiol. 2000 Jul;37(1):42-53
references.pmid: 10931304
references.citation: J Biosci Bioeng. 2000;90(3):313-20
references.pmid: 16232862
references.citation: Biochem J. 1956 Feb;62(2):177-83
references.pmid: 13293170
references.citation: FEMS Microbiol Lett. 1992 Jul 15;73(3):271-6
references.pmid: 1426991
references.citation: Appl Environ Microbiol. 1995 Jan;61(1):109-15
references.pmid: 7887593