title: Detection of methicillin resistance and slime factor production of Staphylococcus aureus in bovine mastitis.
abstract: This study aimed to detect methicillin resistant and slime producing Staphylococcus aureus in cases of bovine mastitis. A triplex PCR was optimized targetting 16S rRNA, nuc and mecA genes for detection of Staphylococcus species, S. aureus and methicillin resistance, respectively. Furthermore, for detection of slime producing strains, a PCR assay targetting icaA and icaD genes was performed. In this study, 59 strains were detected as S. aureus by both conventional tests and PCR, and 13 of them were found to be methicillin resistant and 4 (30.7%) were positive for mecA gene. Although 22 of 59 (37.2%) S. aureus isolates were slime-producing in Congo Red Agar, in PCR analysis only 15 were positive for both icaA and icaD genes. Sixteen and 38 out of 59 strains were positive for icaA and icaD gene, respectively. Only 2 of 59 strains were positive for both methicillin resistance and slime producing, phenotypically, suggesting lack of correlation between methicillin resistance and slime production in these isolates. In conclusion, the optimized triplex PCR in this study was useful for rapid and reliable detection of methicillin resistant S. aureus. Furthermore, only PCR targetting icaA and icaD may not sufficient to detect slime production and further studies targetting other ica genes should be conducted for accurate evaluation of slime production characters of S. aureus strains.
authors.affiliation: Department of Microbiology, University of Ondokuz Mayis, Faculty of Veterinary Medicine , Samsun , TURKEY.
authors.forename: Alper
authors.initials: A
authors.lastname: Ciftci
authors.forename: Arzu
authors.initials: A
authors.lastname: Findik
authors.forename: Ertan Emek
authors.initials: EE
authors.lastname: Onuk
authors.forename: Serap
authors.initials: S
authors.lastname: Savasan
keywords: Staphylococcus aureus; icaA; icaD; mastitis; slime
references.citation: J Infect Dis. 1986 Feb;153(2):332-9
references.pmid: 2935582
references.citation: Emerg Infect Dis. 2005 Dec;11(12):1942-4
references.pmid: 16485485
references.citation: Clin Microbiol Rev. 1988 Apr;1(2):173-86
references.pmid: 3069195
references.citation: Diagn Microbiol Infect Dis. 2005 Jan;51(1):13-7
references.pmid: 15629224
references.citation: J Clin Pathol. 1989 Aug;42(8):872-4
references.pmid: 2475530
references.citation: J Dairy Sci. 2006 Mar;89(3):989-97
references.pmid: 16507693
references.citation: J Dairy Sci. 2000 Apr;83(4):855-62
references.pmid: 10791803
references.citation: J Clin Microbiol. 2001 Jun;39(6):2151-6
references.pmid: 11376050
references.citation: Antimicrob Agents Chemother. 1991 Dec;35(12):2574-9
references.pmid: 1810191
references.citation: Med Microbiol Immunol. 2002 Oct;191(2):101-6
references.pmid: 12410349
references.citation: Cesk Epidemiol Mikrobiol Imunol. 1993 Jun;42(2):51-3
references.pmid: 8348621
references.citation: Infect Immun. 1998 Oct;66(10):4711-20
references.pmid: 9746568
references.citation: Clin Microbiol Infect. 2005 Dec;11(12):967-73
references.pmid: 16307550
references.citation: Vet Microbiol. 2001 Jan 26;78(2):183-91
references.pmid: 11163708
references.citation: J Biomed Mater Res A. 2005 Nov 1;75(2):408-13
references.pmid: 16088896
references.citation: J Antimicrob Chemother. 1999 Jul;44(1):43-55
references.pmid: 10459809
references.citation: Appl Environ Microbiol. 2003 Nov;69(11):6489-94
references.pmid: 14602604
references.citation: Infect Immun. 1999 Oct;67(10):5427-33
references.pmid: 10496925
references.citation: Infect Immun. 1993 Nov;61(11):4857-62
references.pmid: 8406887
references.citation: J Clin Microbiol. 1992 Jul;30(7):1654-60
references.pmid: 1629319
references.citation: J Clin Microbiol. 2000 Jun;38(6):2170-3
references.pmid: 10834971
references.citation: Clin Microbiol Rev. 1997 Oct;10(4):781-91
references.pmid: 9336672
references.citation: Int J Med Microbiol. 2002 Jul;292(2):67-74
references.pmid: 12195737
references.citation: Antimicrob Agents Chemother. 1994 Feb;38(2):345-7
references.pmid: 8192462
references.citation: J Clin Microbiol. 1990 Jun;28(6):1292-6
references.pmid: 2380358
references.citation: Vet Microbiol. 2001 Apr 2;79(3):267-74
references.pmid: 11240104
references.citation: Biomaterials. 2002 Nov;23(21):4233-9
references.pmid: 12194526
references.citation: J Clin Microbiol. 2004 Nov;42(11):4947-55
references.pmid: 15528678
references.citation: Acta Vet Hung. 2003;51(4):451-64
references.pmid: 14680057
references.citation: Vet Microbiol. 2003 Mar 20;92(1-2):179-85
references.pmid: 12488081
references.citation: J Infect. 1999 Nov;39(3):198-204
references.pmid: 10714795
references.citation: J Dairy Sci. 1994 Apr;77(4):970-83
references.pmid: 8201055
references.citation: Int J Antimicrob Agents. 1999 Jan;11(1):47-52
references.pmid: 10075277
references.citation: Emerg Infect Dis. 2001 Mar-Apr;7(2):323-6
references.pmid: 11294733