title: Structural biology: Torqueing about pores.
abstract: Cryo-EM, crystallography, biochemical experiments and computational approaches have been used to study different intermediate states of the Aeromonas hydrophila toxin aerolysin en route to pore formation. These results reveal that an unexpected and marked rotation of the core aerolysin machinery is required to unleash the membrane-spanning regions.
authors.affiliation: Department of Biochemistry and Molecular Biology and the Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, Clayton, Melbourne, Victoria, Australia.
authors.forename: James C
authors.initials: JC
authors.lastname: Whisstock
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Dunstone
mesh_terms: D000333:Aeromonas / Q000737:chemistry*; D001427:Bacterial Toxins / Q000737:chemistry*; D007473:Ion Channels / Q000737:chemistry*; D011498:Protein Precursors / Q000737:chemistry*
chemical_list: D001427:Bacterial Toxins; D007473:Ion Channels; D011498:Protein Precursors
references.citation: Nat Struct Biol. 2002 Oct;9(10):729-33
references.pmid: 12219082
references.citation: J Cell Biol. 1998 Feb 9;140(3):525-40
references.pmid: 9456314
references.citation: J Biol Chem. 1997 May 2;272(18):12170-4
references.pmid: 9115289
references.citation: Nature. 1994 Jan 20;367(6460):292-5
references.pmid: 7510043
references.citation: PLoS Pathog. 2011 Jul;7(7):e1002135
references.pmid: 21779171
references.citation: EMBO J. 2006 Feb 8;25(3):457-66
references.pmid: 16424900
references.citation: PLoS One. 2011;6(6):e20349
references.pmid: 21687664
references.citation: EMBO J. 1992 Jul;11(7):2457-63
references.pmid: 1378390
references.citation: Structure. 2013 Jul 2;21(7):1097-1106
references.pmid: 23810695
references.citation: Nat Chem Biol. 2013 Oct;9(10):623-9
references.pmid: 23912165