title: Use of quantitative microbial risk assessment when investigating foodborne illness outbreaks: the example of a monophasic Salmonella Typhimurium 4,5,12:i:- outbreak implicating beef burgers.
abstract: A major community outbreak of salmonellosis occurred in France in October 2010. Classical epidemiological investigations led to the identification of beef burgers as the cause of the outbreak and the presence of the emerging monophasic Salmonella Typhimurium 4,5,12:i:-. The objective of this study was to understand the events that led to this large outbreak, that is to say, what are the contributing factors associated with consumer exposure to Salmonella. To this end, intensive microbiological investigations on several beef burgers were conducted and a risk assessment model was built. The microbiological results confirm the presence of Salmonella in all analysed frozen burgers at high levels of contamination above 1000 MPN/g. These results in frozen burgers combined with a model of thermal destruction were used to estimate the dose ingested by the exposed persons. Most people that consumed cooked beef burgers were exposed from 1.6 to 3.1 log₁₀ (MPN). The number of sick people predicted with a dose-response relationship for Salmonella is consistent with the observed number of salmonellosis cases. The very high initial contamination level in frozen beef burgers is the primary cause of this large outbreak rather than bad cooking practices. Intensive investigations, modelling of the initial contamination and quantitative exposure and risk assessments are complementary to epidemiological investigation. They can be valuable elements for the assessment of missing information or the identification of the primary causes of outbreaks.
authors.affiliation: Laboratoire de sécurité des aliments, ANSES, F-94000, Maisons-Alfort, France. Electronic address: laurent.guillier@anses.fr.
authors.forename: Laurent
authors.initials: L
authors.lastname: Guillier
authors.forename: Corinne
authors.initials: C
authors.lastname: Danan
authors.forename: Hélène
authors.initials: H
authors.lastname: Bergis
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Delignette-Muller
authors.forename: Sophie
authors.initials: S
authors.lastname: Granier
authors.forename: Sylvie
authors.initials: S
authors.lastname: Rudelle
authors.forename: Annie
authors.initials: A
authors.lastname: Beaufort
authors.forename: Anne
authors.initials: A
authors.lastname: Brisabois
mesh_terms: D000818:Animals; D058491:Bacterial Load; D015373:Bacterial Typing Techniques; D002417:Cattle; D004196:Disease Outbreaks; D005516:Food Microbiology; D005602:France / Q000453:epidemiology; D006801:Humans; D008460:Meat / Q000382:microbiology*; D008962:Models, Theoretical; D015203:Reproducibility of Results; D018570:Risk Assessment; D012478:Salmonella Food Poisoning / Q000453:epidemiology* / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000502:physiology*
keywords: Dose–response; Outbreak investigation; Salmonellosis