title: Four products from Escherichia coli pseudogenes increase hydrogen production.
abstract: Pseudogenes are considered to be nonfunctional genes that lack a physiological role. By screening 3985 Escherichia coli mutants using chemochromic membranes, we found four pseudogenes involved in hydrogen metabolism. Knockouts of pseudogenes ydfW and ypdJ had a defective hydrogen phenotype on glucose and formate, respectively. Also, the knockout of pseudogene yqiG formed hydrogen from formate but not from glucose. For the yqiG mutant, 100% hydrogen recovery was obtained by the complementation of YqiG via a plasmid. The knockout of pseudogene ylcE showed hydrogen deficiency in minimal media which suggested that the role of YlcE is associated with cell growth. Hence, the products of these four pseudogenes play an important physiological role in hydrogen production in E. coli.
authors.affiliation: Department of Biological Functions and Engineering, Graduate School of Life Science and System Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0196, Japan; Department of Chemical Engineering, Pennsylvania State University, 161 Fenske Laboratory, University Park, PA 16802, USA; Department of Biochemistry and Molecular Biology, Pennsylvania State University, 161 Fenske Laboratory, University Park, PA 16802, USA; Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Mohd Zulkhairi
authors.initials: MZ
authors.lastname: Mohd Yusoff
authors.forename: Yuya
authors.initials: Y
authors.lastname: Hashiguchi
authors.forename: Toshinari
authors.initials: T
authors.lastname: Maeda
authors.forename: Thomas K
authors.initials: TK
authors.lastname: Wood
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D005561:Formates / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D009154:Mutation; D011544:Pseudogenes
chemical_list: D029968:Escherichia coli Proteins; D005561:Formates; C030544:formic acid; D006859:Hydrogen; D005947:Glucose
keywords: Biohydrogen; Escherichia coli pseudogene; ydfW; ylcE; ypdJ; yqiG