title: [Virus detection in bronchoalveolar lavage fluid of 122 children with severe pneumonia].
abstract: OBJECTIVE
  To investigate the prevalence of viral pathogen in children with severe pneumonia in Hunan.
  
  
  METHODS
  Bronchoalveolar lavage fluid [BALF] were collected from 122 hospitalized children with severe pneumonia in People's Hospital of Hunan province from January 2011 to December 2011. Nested- or reverse transcription Polymerase chain reaction (PCR or RT-PCR) was used to screen Adenovirus (ADV), Human Bocavirus (HBoV), Parainfluenzaviruses1-4 (PIV1-4), Human Respiratory Syneytial virus (RSV), Influenza virus A (IFVA), Influenza virus B (IFVB), Human Rhinovirus(HRV), Human Metapneumovirus (HMPV), human coronaviruses NL63 and HKU1 (HCoV-NL63, HCoV- HKU1).
  
  
  RESULTS
  Among the 122 bronchoalveolar lavage fluid, viral agents were detected in 60 samples(49.1%), among which ADV (40.98%) was the most common virus, followed by RSV (7.37%) and HBoV (7.37%). Two viruses were detected in 21 individual (35%) samples, of which 20 were dual positive for ADV (40%).
  
  
  CONCLUSIONS
  ADV is the most frequently detected viral etiology of severe pneumonia in children in Hunan during this year. And its Coinfection with other respiratory viruses was common.
authors.affiliation: The First Affiliated Hospital of Hunan Normal University, Changsha 410000, China.
authors.forename: Le-Yun
authors.initials: LY
authors.lastname: Xie
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Zhong
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.forename: Zhao-Jun
authors.initials: ZJ
authors.lastname: Duan
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Xie
authors.forename: Han-Chun
authors.initials: HC
authors.lastname: Gao
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Chen
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Deng
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Xiao-Qian
authors.initials: XQ
authors.lastname: Gao
authors.forename: Ni-Guang
authors.initials: NG
authors.lastname: Xiao
mesh_terms: D000260:Adenoviruses, Human / Q000302:isolation & purification; D000293:Adolescent; D001992:Bronchoalveolar Lavage Fluid / Q000821:virology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D011014:Pneumonia / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012621:Seasons; D014780:Viruses / Q000302:isolation & purification*