title: Biliary phosphatidylcholine and lysophosphatidylcholine profiles in sclerosing cholangitis.
abstract: OBJECTIVE
  To analyze phospholipid profiles in intrahepatic bile from patients with primary sclerosing cholangitis (PSC) and secondary sclerosing cholangitis (SSC).
  
  
  METHODS
  Intrahepatic bile specimens collected via endoscopic retrograde cholangiography from 41 patients were analyzed. Fourteen of these patients were diagnosed with PSC, 10 with SSC, 11 with choledocholithiasis or no identifiable biliary disease, and 6 with cholangiocellular carcinoma (CCC). Bile acid, cholesterol, protein, and bilirubin contents as well as pancreas lipase activity in bile were determined by biochemical methods. Phosphatidylcholine (PC) and lysophosphatidylcholine (LPC) species were quantified using nano-electrospray ionization tandem mass spectrometry.
  
  
  RESULTS
  Bile from all the examined patient groups showed a remarkably similar PC and LPC species composition, with only minor statistical differences. Total biliary PC concentrations were highest in controls (8030 ± 1843 μmol/L) and lowest in patients with CCC (1969 ± 981 μmol/L) (P = 0.005, controls vs SSC and CCC, respectively, P < 0.05). LPC contents in bile were overall low (4.2% ± 1.8%). Biliary LPC/PC ratios and ratios of biliary PC to bilirubin, PC to cholesterol, PC to protein, and PC to bile acids showed no intergroup differences.
  
  
  CONCLUSIONS
  PC and LPC profiles being similar in patients with or without sclerosing cholangitis, these phospholipids are likely not of major pathogenetic importance in this disease group.
authors.affiliation: Annika Gauss, Robert Ehehalt, Karl-Heinz Weiss, Yvonne Schaefer, Petra Kloeters-Plachky, Adolf Stiehl, Wolfgang Stremmel, Peter Sauer, Daniel Nils Gotthardt, Gastroenterology and Hepatology Unit, Department of Internal Medicine, University Hospital Heidelberg, 69120 Heidelberg, Germany.
authors.forename: Annika
authors.initials: A
authors.lastname: Gauss
authors.forename: Robert
authors.initials: R
authors.lastname: Ehehalt
authors.forename: Wolf-Dieter
authors.initials: WD
authors.lastname: Lehmann
authors.forename: Gerhard
authors.initials: G
authors.lastname: Erben
authors.forename: Karl-Heinz
authors.initials: KH
authors.lastname: Weiss
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Schaefer
authors.forename: Petra
authors.initials: P
authors.lastname: Kloeters-Plachky
authors.forename: Adolf
authors.initials: A
authors.lastname: Stiehl
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Stremmel
authors.forename: Peter
authors.initials: P
authors.lastname: Sauer
authors.forename: Daniel Nils
authors.initials: DN
authors.lastname: Gotthardt
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001646:Bile / Q000378:metabolism* / Q000382:microbiology; D016022:Case-Control Studies; D015209:Cholangitis, Sclerosing / Q000378:metabolism* / Q000382:microbiology; D005260:Female; D006801:Humans; D008244:Lysophosphatidylcholines / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010713:Phosphatidylcholines / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D055815:Young Adult
chemical_list: D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines
keywords: Bile; Cholangiocellular carcinoma; Lysophosphatidylcholine; Mass spectrometry; Phosphatidylcholine; Primary sclerosing cholangitis; Secondary sclerosing cholangitis
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