title: Vermelhotin, an anti-inflammatory agent, suppresses nitric oxide production in RAW 264.7 cells via p38 inhibition.
abstract: Vermelhotin exhibited potential anti-inflammatory activity through inhibition of nitric oxide production (IC₅₀ = 5.35 ± 0.59 μM) in LPS-stimulated RAW 264.7 macrophage cells. Vermelhotin suppressed expression of inducible nitric oxide synthase (iNOS) at mRNA and protein levels, in a dose-dependent manner. Mechanistic studies revealed that vermelhotin abrogated upstream signaling of iNOS expression by selectively inhibiting p38 phosphorylation, while ERK and JNK activations were not affected.
authors.affiliation: Chemical Biology Program, Chulabhorn Graduate Institute , Kamphaeng Phet 6 Road, Laksi, Bangkok 10210, Thailand.
authors.forename: Acharavadee
authors.initials: A
authors.lastname: Pansanit
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Park
authors.forename: Tamara P
authors.initials: TP
authors.lastname: Kondratyuk
authors.forename: John M
authors.initials: JM
authors.lastname: Pezzuto
authors.forename: Kriengsak
authors.initials: K
authors.lastname: Lirdprapamongkol
authors.forename: Prasat
authors.initials: P
authors.lastname: Kittakoop
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D009569:Nitric Oxide / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors; D011759:Pyrrolidines / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors*
chemical_list: D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D016328:NF-kappa B; D011759:Pyrrolidines; C534203:vermelhotin; D009569:Nitric Oxide; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases