title: Impact of autologous dendritic cell-based immunotherapy (AGS-004) on B- and T-cell subset changes and immune activation in HIV-infected patients receiving antiretroviral therapy.
abstract: We previously reported that a combination of antiretroviral therapy with 4 monthly injections of each patient's own autologous dendritic cells (AGS-004) electroporated with CD40 ligand and with HIV RNA antigens obtained from each patient's own pre-antiretroviral therapy plasma induced HIV-specific CD8 T-cell responses in 10 patients. To assess other AGS-004-induced immune changes, we evaluated the modifications in B- and T-cell subsets and the level of immune activation in these patients. The proportion of Bm1 naive cells was increased along with an augmentation of the proliferation marker Ki67. Memory B-cell frequency, CD4 and CD8 T-cell subsets, regulatory T-cell frequency, and CD38/HLA-DR/PD-1 T-cell activation levels remained unchanged after AGS-004 dendritic cell immunotherapy.
authors.affiliation: *Chronic Viral Illness Service and Research Institute, McGill University Health Centre, Montreal, Quebec, Canada; †Argos Therapeutics, Durham, NC; and ‡Division of Hematology, McGill University Health Centre, Montreal, Quebec, Canada.
authors.forename: Mohammad-Ali
authors.initials: MA
authors.lastname: Jenabian
authors.forename: Charles A
authors.initials: CA
authors.lastname: Nicolette
authors.forename: Irina Y
authors.initials: IY
authors.lastname: Tcherepanova
authors.forename: Mark A
authors.initials: MA
authors.lastname: Debenedette
authors.forename: Norbert
authors.initials: N
authors.lastname: Gilmore
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Routy
mesh_terms: D016175:B-Lymphocyte Subsets / Q000502:physiology*; D018791:CD4 Lymphocyte Count; D003713:Dendritic Cells / Q000502:physiology*; D015658:HIV Infections / Q000276:immunology / Q000628:therapy*; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D012367:RNA, Viral; D016176:T-Lymphocyte Subsets / Q000502:physiology*; D019562:Viral Load; D014779:Virus Replication
chemical_list: D012367:RNA, Viral