title: The effects of chloroform, ethyl acetate and methanolic extracts of Brassica rapa L. on cell-mediated immune response in mice.
abstract: Turnips with a long history of usage, are helpful in preventing breast and prostate cancer, inflammation and body`s immune system dysfunction. In this study, we investigated the effects of chloroform, ethyl acetate and methanolic extracts of Brassica rapa L. on cell-mediated immune response in mice. Chloroform, ethyl acetate and methanolic extracts of B. rapa glands were prepared by maceration method. To study the effects of B. rapa on acquired immunity, groups of Balb/c mice (n=8) were used. Sheep red blood cell (SRBC) was injected (s.c., 1×10(8)cells/ml, 0.02 ml) and 5 days later, different extracts (10, 100 and 500 mg/kg), betamethasone (4 mg/kg) and Levamisol (4 mg/kg) as a positive control and normal saline as a negative control were given i.p. After 1 h SRBC was injected to footpad (s.c., 1×10(8)cells/ml, 0.02 ml) and footpad swelling was measured up to 72 h. To investigate the effects of B. rapa on innate immunity the same procedure was used, but animals only received one injection of SRBC 1 h after i.p. injection of test compounds. Our findings showed that SRBC induced an increase in paw swelling with maximum response at 6-8 and 2-4 h for innate and acquired immunity, respectively. Betamethasone inhibited and levamisol increased paw thickness in both models. In both innate and acquired immunity models, chloroform, ethyl acetate and methanolic extracts of B. rapa glands significantly and dose-dependently reduced paw thickness. Ethyl acetate extract showed better effect. As glucosinolates are better extracted by ethyl acetate, it may be concluded that they are contributed in the more pronounced effects of ethyl acetate extract.
authors.affiliation: Department of Pharmacology and Toxicology and Isfahan Pharmaceutical Sciences Researches Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
authors.forename: A
authors.initials: A
authors.lastname: Jafarian-Dehkordi
authors.forename: B
authors.initials: B
authors.lastname: Zolfaghari
authors.forename: M
authors.initials: M
authors.lastname: Mirdamadi
keywords: Acquired immunity; Brassica rapa; Innate immunity; Turnip
references.citation: Nat Immunol. 2012 Feb 16;13(3):214-22
references.pmid: 22344284
references.citation: Cancer Res. 1989 Sep 1;49(17):4803-8
references.pmid: 2758413
references.citation: Adv Data. 2004 May 27;(343):1-19
references.pmid: 15188733
references.citation: Carcinogenesis. 1995 May;16(5):1191-4
references.pmid: 7767984
references.citation: Am J Clin Nutr. 1994 May;59(5 Suppl):1166S-1170S
references.pmid: 8172118
references.citation: J Ethnopharmacol. 2000 Jul;71(1-2):193-200
references.pmid: 10904163
references.citation: Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6704-9
references.pmid: 10841567
references.citation: J Immunol Methods. 1984 Apr 27;69(2):149-63
references.pmid: 6232322
references.citation: Int J Sports Med. 1999 May;20(4):258-62
references.pmid: 10376483
references.citation: Phytomedicine. 2007 Aug;14(7-8):538-45
references.pmid: 17084602
references.citation: Molecules. 2010 Dec 30;16(1):251-80
references.pmid: 21193847
references.citation: Immunol Invest. 1992 Jul;21(4):285-96
references.pmid: 1398778
references.citation: J Ethnopharmacol. 2004 Jul;93(1):123-32
references.pmid: 15182917
references.citation: Immunopharmacol Immunotoxicol. 2003 Aug;25(3):451-9
references.pmid: 19180807
references.citation: Mol Cell Biochem. 2005 Oct;278(1-2):101-9
references.pmid: 16180095
references.citation: Pharmacol Ther. 1993 Nov;60(2):301-13
references.pmid: 8022861
references.citation: Trends Biotechnol. 2002 Dec;20(12):522-31
references.pmid: 12443874
references.citation: Biochem Biophys Res Commun. 2007 Dec 7;364(1):181-6
references.pmid: 17942079
references.citation: Plant Methods. 2008 May 20;4:10
references.pmid: 18492252
references.citation: Life Sci. 2007 Mar 27;80(16):1525-38
references.pmid: 17336338
references.citation: Rev Med Chir Soc Med Nat Iasi. 2006 Oct-Dec;110(4):1020-6
references.pmid: 17438919
references.citation: J Ethnopharmacol. 2008 Jun 19;118(1):26-34
references.pmid: 18440170
references.citation: J Ethnopharmacol. 2004 Feb;90(2-3):339-46
references.pmid: 15013199