title: Production of peptide antibiotics by Bacillus sp. GU 057 indigenously isolated from saline soil.
abstract: A total of 112 soil samples were taken from differents areas of district D.I.Khan and Kohat (KPK) Pakistan and screened for production of antibiotics against the Micrococcus luteus and Staphylococcus aureus. Widest zone of inhibition (18mm) was produced by microorganism isolated from saline soil. The strain was later identified as Bacillus GU057 by standard biochemical assays. Maximum activity (18mm inhibition zone) was observed against Staphylococcus aureus after 48 hours of incubation at pH 8 and 4% concentration of glucose. The antibiotic was identified by autobiography as bacitracin. The Bacillus strain GU057 was confirmed as good peptide antibiotic producer and can effectively be indulged as biocontrol agent.
authors.affiliation: Gomal Centre of Biochemistry and Biotechnology (GCBB) Gomal University , D.I.Khan (KPK) , Pakistan.
authors.forename: Adnan
authors.initials: A
authors.lastname: Amin
authors.forename: Muhammad Ayaz
authors.initials: MA
authors.lastname: Khan
authors.forename: Malik
authors.initials: M
authors.lastname: Ehsanullah
authors.forename: Uzma
authors.initials: U
authors.lastname: Haroon
authors.forename: Sheikh Muhammad Farooq
authors.initials: SM
authors.lastname: Azam
authors.forename: Abdul
authors.initials: A
authors.lastname: Hameed
keywords: Bacillus species; Peptide antibiotics; optimization of parameters; screening
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