title: Super-paramagnetic responsive nanofibrous scaffolds under static magnetic field enhance osteogenesis for bone repair in vivo.
abstract: A novel nanofibrous composite scaffold composed of super-paramagnetic γ-Fe2O3 nanoparticles (MNP), hydroxyapatite nanoparticles (nHA) and poly lactide acid (PLA) was prepared using electrospinning technique. The scaffold well responds extern static magnetic field with typical saturation magnetization value of 0.049 emu/g as well as possesses nanofibrous architecture. The scaffolds were implanted in white rabbit model of lumbar transverse defects. Permanent magnets are fixed in the rabbit cages to provide static magnetic field for the rabbits post surgery. Results show that MNP incorporated in the nanofibers endows the scaffolds super-paramagnetic responsive under the applied static magnetic field, which accelerates new bone tissue formation and remodeling in the rabbit defect. The scaffold also exhibits good compatibility of CK, Cr, ALT and ALP within normal limits in the serum within 110 days post implantation. In conclusion, the super-paramagnetic responding scaffold with applying of external magnetic field provides a novel strategy for scaffold-guided bone repair.
authors.affiliation: 1] Department of Biomedical Engineering, Institute of Basic Medical Sciences, Chinese Academy of Medical Science & Peking Union Medical College [2].
authors.forename: Jie
authors.initials: J
authors.lastname: Meng
authors.forename: Bo
authors.initials: B
authors.lastname: Xiao
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.forename: Huadan
authors.initials: H
authors.lastname: Xue
authors.forename: Jing
authors.initials: J
authors.lastname: Lei
authors.forename: Hua
authors.initials: H
authors.lastname: Kong
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Huang
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Jin
authors.forename: Ning
authors.initials: N
authors.lastname: Gu
authors.forename: Haiyan
authors.initials: H
authors.lastname: Xu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001861:Bone Regeneration; D003094:Collagen / Q000378:metabolism; D020007:Implants, Experimental; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles / Q000648:ultrastructure; D008422:Materials Testing; D057139:Nanofibers / Q000648:ultrastructure; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000528:radiation effects; D011817:Rabbits; D013997:Time Factors; D054457:Tissue Scaffolds; D055114:X-Ray Microtomography
chemical_list: D001672:Biocompatible Materials; D058185:Magnetite Nanoparticles; D015675:Osteocalcin; D003094:Collagen
references.citation: Arch Biochem Biophys. 2001 Nov 1;395(1):41-8
references.pmid: 11673864
references.citation: Injury. 2007 Sep;38 Suppl 4:S3-6
references.pmid: 18224731
references.citation: Philos Trans A Math Phys Eng Sci. 2010 Apr 28;368(1917):2065-81
references.pmid: 20308115
references.citation: J Biomech. 2000 Jan;33(1):45-51
references.pmid: 10609517
references.citation: J Orthop Res. 2004 Jul;22(4):709-15
references.pmid: 15183425
references.citation: Int J Immunopathol Pharmacol. 2011 Jan-Mar;24(1 Suppl 2):17-20
references.pmid: 21669132
references.citation: Med Eng Phys. 1998 Sep;20(6):397-402
references.pmid: 9796945
references.citation: Clin Orthop Relat Res. 1987 Sep;(222):300-6
references.pmid: 3621734
references.citation: Biochem Biophys Res Commun. 2003 Nov 7;311(1):32-8
references.pmid: 14575691
references.citation: J Bone Miner Res. 2002 Oct;17(10):1814-21
references.pmid: 12369785
references.citation: Biomaterials. 2011 Dec;32(36):9622-9
references.pmid: 21944829
references.citation: J Orthop Surg Res. 2012 Jun 08;7:24
references.pmid: 22681718
references.citation: PLoS One. 2012;7(6):e38710
references.pmid: 22685602
references.citation: Adv Healthc Mater. 2012 Jan 11;1(1):10-25
references.pmid: 23184683
references.citation: Arch Med Res. 2011 May;42(4):268-73
references.pmid: 21820604
references.citation: Nanoscale. 2010 Dec;2(12):2565-9
references.pmid: 20949222
references.citation: Biochem J. 1984 May 1;219(3):1057-9
references.pmid: 6743242
references.citation: Surgeon. 2012 Aug;10(4):230-9
references.pmid: 22682580
references.citation: Adv Healthc Mater. 2012 Jul;1(4):393-412
references.pmid: 23184771
references.citation: Ther Deliv. 2011 Sep;2(9):1189-98
references.pmid: 22833912
references.citation: J Dent Res. 1992 Dec;71(12):1920-5
references.pmid: 1452895
references.citation: Nat Rev Rheumatol. 2009 Dec;5(12):685-97
references.pmid: 19901916
references.citation: Spine (Phila Pa 1976). 1997 Oct 15;22(20):2351-6
references.pmid: 9355215
references.citation: J Appl Biomater Biomech. 2010 May-Aug;8(2):57-67
references.pmid: 20740467
references.citation: Platelets. 1993;4(6):341-2
references.pmid: 21043604
references.citation: J Bone Joint Surg Am. 1984 Jun;66(5):693-8
references.pmid: 6373772
references.citation: Am J Sports Med. 2000 Nov-Dec;28(6):888-92
references.pmid: 11101114
references.citation: J R Soc Interface. 2013 Jan 09;10(80):20120833
references.pmid: 23303218
references.citation: Biopolymers. 1984 Jul;23(7):1261-7
references.pmid: 6466766
references.citation: Biosci Rep. 1982 Dec;2(12):1025-9
references.pmid: 7165793
references.citation: Int J Nanomedicine. 2012;7:3365-78
references.pmid: 22848165
references.citation: Orthopedics. 1998 Mar;21(3):297-302
references.pmid: 9547814
references.citation: Adv Drug Deliv Rev. 2012 Sep;64(12):1129-41
references.pmid: 22579758
references.citation: Nature. 1981 Jan 1;289(5793):91-3
references.pmid: 7453812
references.citation: J Exp Biol. 2001 Oct;204(Pt 19):3389-99
references.pmid: 11606612
references.citation: Blood. 1993 Aug 15;82(4):1328-34
references.pmid: 8353291
references.citation: J Nanosci Nanotechnol. 2011 Apr;11(4):3039-57
references.pmid: 21776670