title: Proteomics in veterinary medicine: applications and trends in disease pathogenesis and diagnostics.
abstract: Advancement in electrophoresis and mass spectrometry techniques along with the recent progresses in genomics, culminating in bovine and pig genome sequencing, widened the potential application of proteomics in the field of veterinary medicine. The aim of the present review is to provide an in-depth perspective about the application of proteomics to animal disease pathogenesis, as well as its utilization in veterinary diagnostics. After an overview on the various proteomic techniques that are currently applied to veterinary sciences, the article focuses on proteomic approaches to animal disease pathogenesis. Included as well are recent achievements in immunoproteomics (ie, the identifications through proteomic techniques of antigen involved in immune response) and histoproteomics (ie, the application of proteomics in tissue processed for immunohistochemistry). Finally, the article focuses on clinical proteomics (ie, the application of proteomics to the identification of new biomarkers of animal diseases).
authors.affiliation: PhD, DVM, Department of Veterinary Science and Public Health, University of Milano, Via Celoria 10, 20133, Milano, Italy. Email: fabrizio.ceciliani@unimi.it.
authors.forename: F
authors.initials: F
authors.lastname: Ceciliani
authors.forename: D
authors.initials: D
authors.lastname: Eckersall
authors.forename: R
authors.initials: R
authors.lastname: Burchmore
authors.forename: C
authors.initials: C
authors.lastname: Lecchi
mesh_terms: D000820:Animal Diseases / Q000175:diagnosis* / Q000209:etiology / Q000473:pathology; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002415:Cats; D002417:Cattle; D003141:Communicable Diseases / Q000175:diagnosis / Q000209:etiology / Q000662:veterinary*; D004285:Dogs; D006736:Horses; D040901:Proteomics / Q000379:methods; D012418:Ruminants; D013552:Swine; D014730:Veterinary Medicine / Q000379:methods*
chemical_list: D015415:Biomarkers
keywords: 2-dimensional polyacrylamide gel electrophoresis; biomarker discovery; diagnostics; difference gel electrophoresis; immunoproteomics; liquid chromatography–tandem mass spectrometry; matrix-assisted laser desorption/ionization–time of flight; proteomics