title: Use of coated silver nanoparticles to understand the relationship of particle dissolution and bioavailability to cell and lung toxicological potential.
abstract: Since more than 30% of consumer products that include engineered nanomaterials contain nano-Ag, the safety of this material is of considerable public concern. In this study, Ag nanoparticles (NPs) are used to demonstrate that 20 nm polyvinylpyrrolidone (PVP or P) and citrate (C)-coated Ag NPs induce more cellular toxicity and oxidative stress than larger (110 nm) particles due to a higher rate of dissolution and Ag bioavailability. Moreover, there is also a higher propensity for citrate 20 nm (C20) nanoparticles to generate acute neutrophilic inflammation in the lung and to produce chemokines compared to C110. P110 has less cytotoxic effects than C110, likely due to the ability of PVP to complex released Ag(+) . In contrast to the more intense acute pulmonary effects of C20, C110 induces mild pulmonary fibrosis at day 21, likely as a result of slow but persistent Ag(+) release leading to a sub-chronic injury response. Interestingly, the released metallic Ag is incorporated into the collagen fibers depositing around airways and the lung interstitium. Taken together, these results demonstrate that size and surface coating affect the cellular toxicity of Ag NPs as well as their acute versus sub-chronic lung injury potential.
authors.affiliation: Center for Environmental Implications of Nanotechnology, California NanoSystems Institute, University of California at Los Angeles, Los Angeles, CA, 90095, USA.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Ji
authors.forename: Chong Hyun
authors.initials: CH
authors.lastname: Chang
authors.forename: Haiyuan
authors.initials: H
authors.lastname: Zhang
authors.forename: Meiying
authors.initials: M
authors.lastname: Wang
authors.forename: Yu-Pei
authors.initials: YP
authors.lastname: Liao
authors.forename: Sijie
authors.initials: S
authors.lastname: Lin
authors.forename: Huan
authors.initials: H
authors.lastname: Meng
authors.forename: Ruibin
authors.initials: R
authors.lastname: Li
authors.forename: Bingbing
authors.initials: B
authors.lastname: Sun
authors.forename: Laura Van
authors.initials: LV
authors.lastname: Winkle
authors.forename: Kent E
authors.initials: KE
authors.lastname: Pinkerton
authors.forename: Jeffrey I
authors.initials: JI
authors.lastname: Zink
authors.forename: Tian
authors.initials: T
authors.lastname: Xia
authors.forename: André E
authors.initials: AE
authors.lastname: Nel
mesh_terms: D000818:Animals; D001682:Biological Availability; D002460:Cell Line; D005260:Female; D006801:Humans; D008168:Lung / Q000187:drug effects*; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D046529:Microscopy, Electron, Transmission; D051381:Rats; D012834:Silver / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity; D012995:Solubility; D059550:Toxicity Tests, Subchronic
chemical_list: D012834:Silver
keywords: cytotoxicity; dissolution; lung inflammation; nanoparticle; silver
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