title: Determination of metoprolol and its two metabolites in human plasma and urine by high performance liquid chromatography with fluorescence detection and its application in pharmacokinetics.
abstract: A simple, specific and sensitive HPLC method has been developed for the determination of metoprolol and its two metabolites in human plasma and urine. Separation of metoprolol, α-hydroxymetoprolol, O-desmethylmetoprolol and esmolol (internal standard) was achieved on an Agilent XDB-C18 column (150mm×4.6mm, 5μm) using fluorescence detection with Ex 216nm and Em 312nm. The mobile phase consisted of ACN-H2O-0.1%TFA. The analysis was performed in less than 16min with a flow rate of 0.8mL/min. The assay was linear over the concentration range of 5-600ng/mL and 2.5-300ng/mL for metoprolol and its metabolites, respectively. The LOQ were 5.0 and 2.5ng/mL for plasma and urine, respectively. Good precision and accuracy for metoprolol and its two metabolites were obtained. The extraction recoveries were found to be more than 86.91% both in plasma and urine. At the same time, the method was successfully applied to nine healthy volunteers who had been given an oral tablet of 100mg metoprolol.
authors.affiliation: School of Pharmacy of Wenzhou Medical University, 325035 Wenzhou, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.forename: Shihui
authors.initials: S
authors.lastname: Bao
authors.forename: Peiwu
authors.initials: P
authors.lastname: Geng
authors.forename: Jun
authors.initials: J
authors.lastname: Luo
authors.forename: Lei
authors.initials: L
authors.lastname: Yu
authors.forename: Peipei
authors.initials: P
authors.lastname: Pan
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.forename: Guoxin
authors.initials: G
authors.lastname: Hu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D016014:Linear Models; D008790:Metoprolol / Q000031:analogs & derivatives / Q000097:blood* / Q000493:pharmacokinetics / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D008790:Metoprolol
keywords: HPLC-FLD detection; Human plasma and urine; Metoprolol; O-desmethylmetoprolol; Pharmacokinetics; α-Hydroxymetoprolol