title: Horizontol dissemination of TEM- and SHV-typr beta-lactamase genes-carrying resistance plasmids amongst clonical isolates of Enterobacteriaceae.
abstract: The extended-spectrum β-lactamase (ESBL)-producing bacteria have been isolated at increasing frequency worldwide. Expression of ESBL is often associated with multidrug resistance and dissemination by resistance plasmids. During a two-month period in 2000, 133 clinical isolates of enterobacterial strains were randomly collected from outpatients and inpatients at a university hospital in Turkey. The ESBL producing strains were determined by double-disk synergy (DDS) testing. Twenty ESBL producing strains (15%) including Escherichia coli (n = 9), Klebsiella pneumoniae (n = 7), Klebsiella oxytoca (n = 2) and Enterobacter aerogenes (n = 2) were detected and further analyzed for their resistance transfer features, plasmid profile and nature of the resistance genes. Plasmid transfer assays were performed using broth mating techniques. TEM- and SHV- genes were analyzed by polymerase chain reaction (PCR) and hybridization using specific probes. EcoRI restriction enzyme analyses of R plasmids were used in the detection of epidemic plasmids. Fourteen plasmid profiles (A, B1, B2, C1, and C2 to L) were obtained with EcoRI restriction enzyme analysis. Most of these plasmids were detected to carry both TEM- and SHV-derived genes by PCR, and confirmed by localizing each gene by hybridization assay. Epidemiological evidence indicated that there was an apparent horizontal dissemination of conjugative R plasmids among multidrug-resistant enterobacterial genera and species in this hospital.
authors.affiliation: Department of Biology, Faculty of Arts and Sciences, Rize University , 53100 Rize ; Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Karadeniz Technical University , 61080 Trabzon , Turkey.
authors.forename: Osman Birol
authors.initials: OB
authors.lastname: Ozgumus
authors.forename: Ilknur
authors.initials: I
authors.lastname: Tosun
authors.forename: Faruk
authors.initials: F
authors.lastname: Aydin
authors.forename: Ali Osman
authors.initials: AO
authors.lastname: Kilic
keywords: ESBL; Enterobacteriaceae; horizontal spread; resistance plasmids
references.citation: J Hosp Infect. 1995 Nov;31(3):177-87
references.pmid: 8586786
references.citation: Clin Infect Dis. 1997 Jan;24 Suppl 1:S19-45
references.pmid: 8994778
references.citation: J Clin Microbiol. 1993 Feb;31(2):179-84
references.pmid: 8432800
references.citation: Plasmid. 1978 Jun;1(3):417-20
references.pmid: 372973
references.citation: J Antimicrob Chemother. 1982 Apr;9(4):275-86
references.pmid: 6979537
references.citation: Jpn J Infect Dis. 2005 Jun;58(3):162-7
references.pmid: 15973008
references.citation: Infect Dis Clin North Am. 1997 Dec;11(4):875-87
references.pmid: 9421705
references.citation: Curr Opin Pharmacol. 2005 Oct;5(5):452-8
references.pmid: 16084766
references.citation: Antimicrob Agents Chemother. 1990 Feb;34(2):219-24
references.pmid: 2327769
references.citation: JAMA. 1999 Feb 10;281(6):563-4
references.pmid: 10022115
references.citation: Antimicrob Agents Chemother. 1995 Jun;39(6):1211-33
references.pmid: 7574506
references.citation: JAMA. 1999 Feb 10;281(6):517-23
references.pmid: 10022107
references.citation: J Antimicrob Chemother. 2005 Jul;56(1):52-9
references.pmid: 15917288
references.citation: FEMS Microbiol Lett. 1991 Jul 15;66(1):19-25
references.pmid: 1936934
references.citation: Antimicrob Agents Chemother. 2001 Jan;45(1):88-95
references.pmid: 11120950
references.citation: Antimicrob Agents Chemother. 1991 Sep;35(9):1697-704
references.pmid: 1952834
references.citation: Jpn J Infect Dis. 2004 Apr;57(2):37-40
references.pmid: 15118205
references.citation: J Clin Microbiol. 2001 May;39(5):1865-70
references.pmid: 11326005
references.citation: Antimicrob Agents Chemother. 1998 Dec;42(12):3117-22
references.pmid: 9835501
references.citation: J Clin Microbiol. 1996 Feb;34(2):358-63
references.pmid: 8789016
references.citation: Antimicrob Agents Chemother. 1997 Oct;41(10):2265-9
references.pmid: 9333059
references.citation: Antimicrob Agents Chemother. 1992 Sep;36(9):1981-6
references.pmid: 1329636
references.citation: Clin Infect Dis. 2000 Jan;30(1):55-60
references.pmid: 10619733
references.citation: J Mol Biol. 1975 Nov 5;98(3):503-17
references.pmid: 1195397
references.citation: Rev Infect Dis. 1988 Jul-Aug;10(4):867-78
references.pmid: 3263690