title: Probiotic characterization of potential hydrolases producing Lactococcus lactis subsp. lactis isolated from pickled yam.
abstract: The aim of this study was to characterize potential probiotic strain co-producing α-amylase and β-galactosidase. Sixty-three strains, isolated from pickle samples were screened for their hydrolase producing capacity by utilizing different starches as carbon source. One out of 63 strains, isolated from traditionally fermented pickled yam showing maximum hydrolase activity (α-amylase (36.9 U/ml) and β-galactosidase (42.6 U/ml)) within a period of 48 hours was identified as Lactococcus lactis subsp. lactis. Further, it was assessed for the probiotic characteristics under gastrointestinal conditions like acidic, alkaline, proteolytic enzymes, bile stress and found to exhibit tolerance to these stresses. The therapeutic potential of the isolate is implicated because of its antagonistic effect against enteric foodborne pathogens (Salmonella typhimurium, Escherichia coli 0157:H7, Staphylococcus aureus, Yersinia enterocolitica and Aeromonas hydrophila). The results of this study entail a potential applicability of the isolate in developing future probiotic foods besides the production of industrially significant hydrolases.
authors.affiliation: Department of Biotechnology & Environmental Sciences, Thapar University , Patiala, Punjab , India.
authors.forename: Seema
authors.initials: S
authors.lastname: Bhanwar
authors.forename: Arashdeep
authors.initials: A
authors.lastname: Singh
authors.forename: Abhijit
authors.initials: A
authors.lastname: Ganguli
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000191:economics / Q000302:isolation & purification / Q000378:metabolism; D004032:Diet / Q000208:ethnology; D004063:Digestion; D027742:Dioscorea / Q000737:chemistry / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial; D057141:Food, Preserved / Q000191:economics / Q000382:microbiology*; D005525:Food-Processing Industry / Q000191:economics; D005750:Gastric Juice / Q000382:microbiology; D007194:India; D007220:Industrial Waste / Q000191:economics; D013294:Lactococcus lactis / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D056265:Microbial Interactions; D050296:Microbial Viability; D010189:Pancreatic Juice / Q000382:microbiology; D035281:Plant Tubers / Q000737:chemistry / Q000382:microbiology*; D019936:Probiotics / Q000191:economics / Q000302:isolation & purification / Q000378:metabolism*; D013213:Starch / Q000191:economics / Q000378:metabolism; D000516:alpha-Amylases / Q000191:economics / Q000302:isolation & purification / Q000378:metabolism*; D001616:beta-Galactosidase / Q000191:economics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D007220:Industrial Waste; D013213:Starch; D000516:alpha-Amylases; D001616:beta-Galactosidase