title: Protective effects of the Morus alba L. leaf extracts on cisplatin-induced nephrotoxicity in rat.
abstract: Cisplatin (CP) as an important anti-tumor drug causes nephrotoxicity mainly by oxidative stress and renin-angiotensin system (RAS). Since flavonoids have high antioxidant activity and probable role in the inhibition of RAS, this study was designed to investigate the protective effect of hydroalcoholic extract and flavonoid fraction of Morus alba leaves on cisplatin-induced nephrotoxicity in rat. Extracts of Morus alba leaves were prepared and analyzed Phytochemically. Male rats (160-200 g) were used in this study (n=7-9). Normal group received 0.2 ml normal saline intraperitoneally (i.p.) once daily for ten days. Control animals received CP on the third day and saline in the remaining days. Other groups received either hydroalcoholic extract (200, 400 and 600 mg/kg, i.p.) or flavonoid fraction (50, 100 and 200 mg/kg, i.p.) for two days before CP administration and thereafter until tenth day. Serum concentrations of blood urea nitrogen (BUN), creatinine (Cr) and nitric oxide were measured using standard methods. Also left kidneys were prepared for pathological study. The serum levels of BUN and Cr increased in animals received CP. Hydroalcoholic extract was ineffective in reversing these alterations but flavonoid fraction (50 and 100 mg/kg) significantly inhibited CP-induced increases of BUN and Cr. None of the treatments could affect serum concentration of nitric oxide. Flavonoid fraction could also prevent CP-induced pathological damage of the kidney. It seems that concurrent use of flavonoid fraction of Morus alba with CP can protect kidneys from CP-induced nephrotoxicity.
authors.affiliation: Water and Electrolytes Research Centre; Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran ; Department of Pharmacology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
authors.forename: M
authors.initials: M
authors.lastname: Nematbakhsh
authors.forename: V
authors.initials: V
authors.lastname: Hajhashemi
authors.forename: A
authors.initials: A
authors.lastname: Ghannadi
authors.forename: A
authors.initials: A
authors.lastname: Talebi
authors.forename: M
authors.initials: M
authors.lastname: Nikahd
keywords: Cisplatin; Flavonoid fraction; Morus alba; Nephrotoxicity
references.citation: Fitoterapia. 2005 Dec;76(7-8):608-13
references.pmid: 16229967
references.citation: Toxicol Sci. 2000 Feb;53(2):447-57
references.pmid: 10696793
references.citation: Toxins (Basel). 2010 Nov;2(11):2490-518
references.pmid: 22069563
references.citation: J Med Food. 2005 Winter;8(4):488-93
references.pmid: 16379560
references.citation: Arch Pharm Res. 1999 Feb;22(1):81-5
references.pmid: 10071966
references.citation: Chem Pharm Bull (Tokyo). 2010 Feb;58(2):257-60
references.pmid: 20118592
references.citation: Kidney Int. 2008 May;73(9):994-1007
references.pmid: 18272962
references.citation: Plant Foods Hum Nutr. 2009 Jun;64(2):116-21
references.pmid: 19434497
references.citation: J Cardiovasc Pharmacol. 2008 Mar;51(3):239-45
references.pmid: 18356687
references.citation: Chemotherapy. 2009;55(6):399-406
references.pmid: 19955745
references.citation: Res Pharm Sci. 2011 Jan;6(1):13-21
references.pmid: 22049274
references.citation: Kidney Blood Press Res. 2004;27(3):148-58
references.pmid: 15118361
references.citation: Ren Fail. 1995 Nov;17(6):665-74
references.pmid: 8771239
references.citation: Res Pharm Sci. 2011 Jul;6(2):123-8
references.pmid: 22224096
references.citation: J Ethnopharmacol. 2002 Oct;82(2-3):83-7
references.pmid: 12241981
references.citation: Exp Toxicol Pathol. 2009 May;61(3):223-42
references.pmid: 18986801
references.citation: Hypertens Res. 2008 Feb;31(2):175-84
references.pmid: 18360035
references.citation: Am J Med Sci. 2007 Aug;334(2):115-24
references.pmid: 17700201
references.citation: J Pharm Biomed Anal. 2001 Jan;24(3):353-9
references.pmid: 11199214
references.citation: J Ethnopharmacol. 2003 Nov;89(1):67-71
references.pmid: 14522434
references.citation: Pharmacol Rep. 2006 Jul-Aug;58(4):526-32
references.pmid: 16963799
references.citation: Res Pharm Sci. 2012 Apr;7(2):103-10
references.pmid: 23181087
references.citation: Pharmacol Rep. 2009 Jan-Feb;61(1):67-75
references.pmid: 19307694