title: The Ccr4-not protein complex regulates the phase of the Neurospora circadian clock by controlling white collar protein stability and activity.
abstract: In the Neurospora circadian negative feedback loop, white collar 1 (WC-1) and WC-2 form the WC complex that activates frequency (frq) transcription. Here we show that Not1 is a WC-interacting protein and is important for maintaining WC levels. The not1 transcript displays a circadian oscillation with a similar phase as frq. Down-regulation of not1 leads to low levels of WC-1 and WC-2 and a delayed circadian phase as a result of increased protein degradation and increased WC activity. Protein purification of Not1 shows that it is part of the Neurospora Ccr4-Not complex. ccr4 is a clock-controlled gene and is regulated directly by the WC complex. Down-regulation of ccr4 results in a phase delay and period lengthening of the clock. Together, our findings suggest that the Ccr4-Not complex participates in the Neurospora clock function by interacting with and regulating the WC complex.
authors.affiliation: From the Center for Circadian Clocks, Medical College, Soochow University, Suzhou 215123, China.
authors.forename: Guocun
authors.initials: G
authors.lastname: Huang
authors.forename: Qiyang
authors.initials: Q
authors.lastname: He
authors.forename: Jinhu
authors.initials: J
authors.lastname: Guo
authors.forename: Joonseok
authors.initials: J
authors.lastname: Cha
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
mesh_terms: D057906:Circadian Clocks / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism*; D009492:Neurospora crassa / Q000235:genetics / Q000378:metabolism*; D055550:Protein Stability; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; C087405:FRQ protein, Neurospora crassa; D005656:Fungal Proteins; D046912:Multiprotein Complexes; D012097:Repressor Proteins; D014157:Transcription Factors; C105589:wc-1 protein, Neurospora crassa; C103772:white collar 2 protein, Neurospora
keywords: Circadian Clock; Circadian Rhythms; Neurospora; Photobiology; Transcription
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