title: Cloning of the Lentinula edodes B mating-type locus and identification of the genetic structure controlling B mating.
abstract: During the life cycle of heterothallic tetrapolar Agaricomycetes such as Lentinula edodes (Berk.) Pegler, the mating type system, composed of unlinked A and B loci, plays a vital role in controlling sexual development and resulting formation of the fruit body. L. edodes is produced worldwide for consumption and medicinal purposes, and understanding its sexual development is therefore of great importance. A considerable amount of mating type factors has been indicated over the past decades but few genes have actually been identified, and no complete genetic structures of L. edodes B mating-type loci are available. In this study, we cloned the matB regions from two mating compatible L. edodes strains, 939P26 and 939P42. Four pheromone receptors were identified on each new matB region, together with three and four pheromone precursor genes in the respective strains. Gene polymorphism, phylogenetic analysis and distribution of pheromone receptors and pheromone precursors clearly indicate a bipartite matB locus, each sublocus containing a pheromone receptor and one or two pheromone precursors. Detailed sequence comparisons of genetic structures between the matB regions of strains 939P42, 939P26 and a previously reported strain SUP2 further supported this model and allowed identification of the B mating type subloci borders. Mating studies confirmed the control of B mating by the identified pheromone receptors and pheromones in L. edodes.
authors.affiliation: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, P.R. China; Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, P.R. China; National Engineering Research Center of Edible Fungi, Ministry of Science and Technology, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wu
authors.forename: Arend
authors.initials: A
authors.lastname: van Peer
authors.forename: Wenhua
authors.initials: W
authors.lastname: Song
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Mingjie
authors.initials: M
authors.lastname: Chen
authors.forename: Qi
authors.initials: Q
authors.lastname: Tan
authors.forename: Chunyan
authors.initials: C
authors.lastname: Song
authors.forename: Meiyan
authors.initials: M
authors.lastname: Zhang
authors.forename: Dapeng
authors.initials: D
authors.lastname: Bao
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D049770:Genes, Mating Type, Fungal / Q000235:genetics*; D056426:Genetic Loci / Q000235:genetics*; D008969:Molecular Sequence Data; D030781:Organisms, Genetically Modified; D010802:Phylogeny; D012045:Regulatory Sequences, Nucleic Acid / Q000502:physiology; D017385:Sequence Homology; D020942:Shiitake Mushrooms / Q000235:genetics*
keywords: AA; AF; B mating-type locus; CAAX; CIIA; CIIS; CTAB; CVIL; CVIS; CVVA; Cc; Cetyltrimethyl Ammonium Bromide; Cn; Coprinopsis cinerea; Cryptococcus neoformans; DDE; E/A; E/H; ER; Flammulina velutipes; Fv; GF; GX; GY; Genetic structure; Laccaria bicolor; Lb; Le; Lentinula edodes; NCBI; National Center for Biotechnology Information; No; PCR; PDA; PDY; PXD; PXN; Pheromone receptor; Potato, Dextrose and Agar medium; Potato, Dextrose and Yeast extract medium; TM; XF; aliphatic amino acids (or alanine)–phenylalanine; amino acid(s); any residue–phenylalanine; aspartic acid–aspartic acid–glutamic acid; base pair(s); bp; cysteine–aliphatic amino acids–aliphatic amino acids–any residue; cysteine–isoleucine–isoleucine–alanine; cysteine–isoleucine–isoleucine–serine; cysteine–valine–isoleucine–leucine; cysteine–valine–isoleucine–serine; cysteine–valine–valine–alanine; dNTP; deoxyribonucleoside triphosphate; glutamic acid/alanine; glutamic acid/histidine; glutamic acid–arginine; glycine–any residue; glycine–phenylalanine; glycine–tyrosine; kb; kilobase(s) or 1000bp; number; polymerase chain reaction; proline–any residue–aspartic acid; proline–any residue–aspartic acid–asparaginate; transmembrane