title: Intravital microscopy for imaging subcellular structures in live mice expressing fluorescent proteins.
abstract: Here we describe a procedure to image subcellular structures in live rodents that is based on the use of confocal intravital microscopy. As a model organ, we use the salivary glands of live mice since they provide several advantages. First, they can be easily exposed to enable access to the optics, and stabilized to facilitate the reduction of the motion artifacts due to heartbeat and respiration. This significantly facilitates imaging and tracking small subcellular structures. Second, most of the cell populations of the salivary glands are accessible from the surface of the organ. This permits the use of confocal microscopy that has a higher spatial resolution than other techniques that have been used for in vivo imaging, such as two-photon microscopy. Finally, salivary glands can be easily manipulated pharmacologically and genetically, thus providing a robust system to investigate biological processes at a molecular level. In this study we focus on a protocol designed to follow the kinetics of the exocytosis of secretory granules in acinar cells and the dynamics of the apical plasma membrane where the secretory granules fuse upon stimulation of the beta-adrenergic receptors. Specifically, we used a transgenic mouse that co-expresses cytosolic GFP and a membrane-targeted peptide fused with the fluorescent protein tandem-Tomato. However, the procedures that we used to stabilize and image the salivary glands can be extended to other mouse models and coupled to other approaches to label in vivo cellular components, enabling the visualization of various subcellular structures, such as endosomes, lysosomes, mitochondria, and the actin cytoskeleton.
authors.affiliation: Intracellular Membrane Trafficking Unit, Oral and Pharyngeal Cancer Branch National Institute of Dental and Craniofacial Research, National Institutes of Health.
authors.forename: Andrius
authors.initials: A
authors.lastname: Masedunskas
authors.forename: Natalie
authors.initials: N
authors.lastname: Porat-Shliom
authors.forename: Muhibullah
authors.initials: M
authors.lastname: Tora
authors.forename: Oleg
authors.initials: O
authors.lastname: Milberg
authors.forename: Roberto
authors.initials: R
authors.lastname: Weigert
mesh_terms: D061354:Acinar Cells / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D005089:Exocytosis; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D012469:Salivary Glands / Q000378:metabolism / Q000648:ultrastructure*; D022142:Secretory Vesicles / Q000378:metabolism / Q000648:ultrastructure*; D013347:Subcellular Fractions / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins
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