title: Cloning and characterization of squalene synthase gene from Poria cocos and its up-regulation by methyl jasmonate.
abstract: Squalene synthase (SQS) catalyzes the condensation of two molecules of farnesyl diphosphate to give presqualene diphosphate and the subsequent rearrangement to form squalene. The gene encoding squalene synthase was cloned from Poria cocos by degenerate PCR and inverse PCR. The open reading frame of the gene is 1,497 bp, which encodes 499 amino acid residues. A phylogenetic analysis revealed that P. cocos SQS belonged to the fungus group, and was more closely related to the SQS of Ganoderma lucidum than other fungi. The treatment of P. cocos with methyl jasmonate (MeJA) significantly enhanced the transcriptional level of P. cocos sqs gene and the content of squalene in P. cocos. The transcriptional level of sqs gene was approximately fourfold higher than the control sample and the squalene content reached 128.62 μg/g, when the concentration of MeJA was 300 μM after 72 h induction.
authors.affiliation: Department of Bioengineering, College of Food Science, South China Agricultural University, 482 Wu-Shan Road, Tian-He District, Guangzhou, 510642, Guangdong, China, believe1234@126.com.
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Wang
authors.forename: Jun-Fang
authors.initials: JF
authors.lastname: Lin
authors.forename: Li-Qiong
authors.initials: LQ
authors.lastname: Guo
authors.forename: Lin-Feng
authors.initials: LF
authors.lastname: You
authors.forename: Xian-Lu
authors.initials: XL
authors.lastname: Zeng
authors.forename: Jia-Ming
authors.initials: JM
authors.lastname: Wen
mesh_terms: D000085:Acetates / Q000378:metabolism*; D003001:Cloning, Molecular; D016000:Cluster Analysis; D003517:Cyclopentanes / Q000378:metabolism*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D013186:Farnesyl-Diphosphate Farnesyltransferase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D054883:Oxylipins / Q000378:metabolism*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D055441:Poria / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013185:Squalene / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D004271:DNA, Fungal; D054883:Oxylipins; D012333:RNA, Messenger; D013185:Squalene; C072239:methyl jasmonate; D013186:Farnesyl-Diphosphate Farnesyltransferase
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