title: New triterpenoid estersaponins from the root barks of Pittosporum verticillatum subsp. verticillatum and evaluation of cytotoxicities.
abstract: The phytochemical investigation of the root barks of Pittosporum verticillatum Bojer subsp. verticillatum led to the isolation of three new triterpene saponins, 3-O-[β-D-glucopyranosyl-(1→2)]-[α-L-arabinopyranosyl-(1→3)]-[α-L-arabinofuranosyl-(1→4)]-β-D-glucuronopyranosyl-21-O-(2-acetoxy-2-methylbutanoyl)-R1-barrigenol (1), 3-O-[β-D-glucopyranosyl-(1→2)]-[α-L-arabinopyranosyl-(1→3)]-[α-L-arabinofuranosyl-(1→4)]-β-D-glucuronopyranosyl-21-O-(2-acetoxy-2-methylbutanoyl)-28-O-acetyl-R1-barrigenol (2), 3-O-[β-D-glucopyranosyl-(1→2)]-[α-L-arabinopyranosyl-(1→3)]-[α-L-arabinofuranosyl-(1→4)]-β-D-glucuronopyranosyl-21-O-β,β-dimethylacryloyl-22-O-angeloyl-R1-barrigenol (3), and one known saponin senaciapittoside B (4). Their structures were elucidated mainly by 1D- and 2D-NMR spectroscopy and HRESIMS. Compounds 1-4 were evaluated for their cytotoxicity against one human cancer cell line (SW480) and one rat cardiomyoblast cell line (H9c2).
authors.affiliation: EA 4267 (FDE/UFC), Laboratoire de Pharmacognosie, Faculté de Pharmacie, Université de Bourgogne, 7, Bd. Jeanne d'Arc, BP 87900, 21079 Dijon Cedex France.
authors.forename: Mahenina Jaovita
authors.initials: MJ
authors.lastname: Manase
authors.affiliation: EA 4267 (FDE/UFC), Laboratoire de Pharmacognosie, Faculté de Pharmacie, Université de Bourgogne, 7, Bd. Jeanne d'Arc, BP 87900, 21079 Dijon Cedex France.
authors.forename: Anne-Claire
authors.initials: AC
authors.lastname: Mitaine-Offer
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Miyamoto
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Tanaka
authors.affiliation: Cohiro, UFR de Médecine de Dijon, 7, Bd. Jeanne d'Arc, BP 87900, 21079 Dijon Cedex, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Delemasure
authors.affiliation: Cohiro, UFR de Médecine de Dijon, 7, Bd. Jeanne d'Arc, BP 87900, 21079 Dijon Cedex, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Dutartre
authors.affiliation: EA 4267 (FDE/UFC), Laboratoire de Pharmacognosie, Faculté de Pharmacie, Université de Bourgogne, 7, Bd. Jeanne d'Arc, BP 87900, 21079 Dijon Cedex France. Electronic address: m-a.lacaille-dubois@u-bourgogne.fr.
authors.forename: Marie-Aleth
authors.initials: MA
authors.lastname: Lacaille-Dubois
mesh_terms: D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D006801:Humans; D015394:Molecular Structure; D009369:Neoplasms / Q000188:drug therapy; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D019685:Rosales / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: C000588654:3-O-(glucopyranosyl-(1-2))-(arabinopyranosyl-(1-3))-(arabinofuranosyl-(1-4))-glucuronopyranosyl-21-O-beta,beta-dimethylacryloyl-22-O-angeloyl-R1-barrigenol; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; C580616:senaciapittoside B
keywords: Cytotoxicity; Pittosporaceae; Pittosporum verticillatum; Triterpene saponin