title: Isolation of Helicobacter pylori in gastric mucosa and susceptibility to five antimicrobial drugs in Southern chile.
abstract: Helicobacter pylori colonizes more than 50% of the world population thus, it is considered an important cause of gastric cancer. The aim of this study was to determine the isolation frequency of H. pylori in Southern Chile from patients with symptomatology compatible with gastritis or gastric ulcer and to correlate these findings with demographic parameters of infected patients and the susceptibility profiles of the isolated strains to the antimicrobial drugs used in the eradication treatments. A total of 240 patients were enrolled in the study. Each gastric biopsy was homogenized and seeded onto blood agar plates containing a selective antibiotics mixture (DENT supplement). Plates were incubated at 37° C in a microaerophilic environment for five days. The susceptibility profiles to amoxicillin, ciprofloxacin, clarithromycin, tetracycline and metronidazole were determined using the E-test method. H. pylori was isolated from 99 patients (41.3%) with slightly higher frequency in female (42% positive cultures) than male (40.2% positive cultures). With regard to age and educational level, the highest isolation frequencies were obtained in patients between 21-30 (55%) and 41-50 (52.6%) years old, and patients with secondary (43.9%) and university (46.2%) educational levels. Nineteen (21.6%) strains showed resistance to at least one antimicrobial drug. Tetracycline was the most active antimicrobial in vitro, whereas metronidazole was the less active. One strain (5.3%) showed resistance to amoxicillin, clarithomycin and metronidazole, simultaneously.
authors.affiliation: Institute of Clinical Microbiology, Faculty of Medicine, Universidad Austral de Chile , Valdivia , Chile.
authors.forename: Laura
authors.initials: L
authors.lastname: Otth
authors.forename: Myra
authors.initials: M
authors.lastname: Wilson
authors.forename: Heriberto
authors.initials: H
authors.lastname: Fernández
authors.forename: Carola
authors.initials: C
authors.lastname: Otth
authors.forename: Claudio
authors.initials: C
authors.lastname: Toledo
authors.forename: Victoria
authors.initials: V
authors.lastname: Cárcamo
authors.forename: Paula
authors.initials: P
authors.lastname: Rivera
authors.forename: Luis
authors.initials: L
authors.lastname: Ruiz
keywords: Helicobacter pylori; antimicrobial susceptibility; gastric ulcer
references.citation: Rev Gastroenterol Peru. 2005 Jan-Mar;25(1):23-41
references.pmid: 15818420
references.citation: Rev Med Chil. 2007 Mar;135(3):287-93
references.pmid: 17505573
references.citation: Rev Med Chil. 2001 Jun;129(6):643-6
references.pmid: 11510204
references.citation: Rev Esp Quimioter. 2001 Jun;14(2):172-6
references.pmid: 11704771
references.citation: Eur J Clin Microbiol Infect Dis. 1993 Oct;12(10):795-7
references.pmid: 8307057
references.citation: Rev Chilena Infectol. 2006 Sep;23(3):249-56
references.pmid: 16896500
references.citation: Antimicrob Agents Chemother. 2007 Jan;51(1):346-9
references.pmid: 17043117
references.citation: J Antimicrob Chemother. 1997 Aug;40(2):279-81
references.pmid: 9301996
references.citation: Gastroenterology. 1999 Dec;117(6):1308-15
references.pmid: 10579972
references.citation: J Clin Microbiol. 2000 Jan;38(1):286-91
references.pmid: 10618103
references.citation: Aliment Pharmacol Ther. 2007 Aug 1;26(3):343-57
references.pmid: 17635369
references.citation: J Microbiol. 2006 Dec;44(6):660-4
references.pmid: 17205045
references.citation: J Med Microbiol. 2002 Aug;51(8):705-709
references.pmid: 12171304
references.citation: BMC Public Health. 2005 Nov 10;5:118
references.pmid: 16283940
references.citation: Int J Epidemiol. 2002 Jun;31(3):624-31
references.pmid: 12055165
references.citation: Aliment Pharmacol Ther. 1995;9 Suppl 2:33-9
references.pmid: 8547526
references.citation: Rev Esp Enferm Dig. 2007 Feb;99(2):63-70
references.pmid: 17417916
references.citation: Lancet. 1983 Jun 4;1(8336):1273-5
references.pmid: 6134060
references.citation: Rev Med Chil. 1989 Oct;117(10):1180-1
references.pmid: 2519366
references.citation: Rev Med Chil. 2003 Nov;131(11):1313-20
references.pmid: 14743695
references.citation: World J Gastroenterol. 2007 Jun 7;13(21):2967-72
references.pmid: 17589948
references.citation: J Clin Microbiol. 1997 Jul;35(7):1842-6
references.pmid: 9196205
references.citation: World J Gastroenterol. 1999 Jun;5(3):263-266
references.pmid: 11819443
references.citation: J Clin Microbiol. 1998 Apr;36(4):1165-6
references.pmid: 9542964
references.citation: Antimicrob Agents Chemother. 2000 Aug;44(8):2207-10
references.pmid: 10898705
references.citation: Rev Med Chil. 2007 Aug;135(8):1009-14
references.pmid: 17989858