title: Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp.
abstract: Solid state fermentation was carried out using various agro- industrial wastes with the best amylase producing strain isolated from soil. Different physicochemical conditions were varied for maximum enzyme production. The strain produced about 5400 units/g of amylase at 1:3 moisture content, 20% inoculum, after 72 h of incubation with Mustard Oil seed cake as the substrate. The optimum temperature and pH of the enzyme activity were found to be 50°C and 6 respectively. The enzyme was found to be thermostable at 70°C for about 2 h without any salt. It showed stability at pH range 5-7. The metal ions as Na(+), Ca(++), Mg(++) and Co(++) enhanced the enzyme activity.
authors.affiliation: Amity Institute of Biotechnology, Amity University , Sector-125, Noida, Uttar Pradesh , India.
authors.forename: Rajshree
authors.initials: R
authors.lastname: Saxena
authors.forename: Rajni
authors.initials: R
authors.lastname: Singh
keywords: Agro-industrial wastes; Amylase; Bacillus sp.; Enzyme optimization; Solid State Fermentation; Stability
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