title: High interstitial fluid pressure promotes tumor cell proliferation and invasion in oral squamous cell carcinoma.
abstract: It has been shown that interstitial fluid pressure (IFP) is elevated in many solid tumors. The elevated IFP in tumors is responsible, at least in part, for the poor blood supply, inadequate delivery of therapeutic agents to solid tumors and poor treatment response in patients. The present study was carried out to examine alterations in malignant phenotypes in oral squamous cell carcinoma cells subjected to conditions mimicking IFP and to identify the relevant molecular mechanisms. We investigated tumor cell proliferation and invasion using SCC-4 and SCC-9 cells subjected to an increased extracellular pressure of 0, 15 and 30 mmHg in vitro. The results revealed that the increased IFP resulted in a marked increase in cancer cell proliferation, survival and invasion in vitro and altered the expression of >1,800 genes involved in invasion and metastasis, the heat shock pathway, the p38 and JNK signaling pathway, apoptosis and the cell growth and differentiation signaling pathway. These results suggest the important potential clinical application of measuring IFP, which can be used as a generic marker of prognosis and response to therapy.
authors.affiliation: Department of Head and Neck Oncology Surgery, Sichuan Cancer Hospital, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yu
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wu
authors.forename: Jinchuan
authors.initials: J
authors.lastname: Fan
authors.forename: Jianchao
authors.initials: J
authors.lastname: Chen
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Guiquan
authors.initials: G
authors.lastname: Zhu
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Wang
authors.forename: Longjiang
authors.initials: L
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000502:physiology; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology*; D002454:Cell Differentiation / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D045604:Extracellular Fluid / Q000378:metabolism*; D006801:Humans; D011312:Pressure