title: Initial performance evaluation of the UniCel® DxH slide maker/stainer Coulter® cellular analysis system.
abstract: BACKGROUND
  Despite the advancements in instrumentation within hematology laboratories, there is still a need for review of a peripheral blood film (PBF). For a thorough PBF evaluation, it is critical that a well spread and stained film is available.
  
  
  METHODS
  In this study, we evaluated an automatic slide maker/stainer (DxH-SMS, Beckman Coulter) compared with manually prepared blood films on 124 normal and abnormal samples. The primary goal of the study was to determine whether or not the DxH-SMS was able to consistently and reproducibly prepare and stain blood films of exemplary quality, without carryover between specimens. Additionally, repeatability of white blood cell distribution, comparability of morphology to reference methodologies, and grading of acceptance criteria outlined in the CLSI document H20-A2 were assessed.
  
  
  RESULTS
  Carryover was not an issue and repeatability was within expected limits. There was excellent agreement of the 5-part differential between the automated blood films made by the DxH-SMS compared with the manually prepared reference blood film. There was no difference in identification and enumeration of blasts, variant lymphocytes, or nucleated red blood cells (P < 0.05). Red cell morphology showed excellent agreement.
  
  
  CONCLUSIONS
  Blood films prepared by the DxH-SMS are of excellent quality, reproducible, and compare well with manually prepared slides. Introduction to our laboratory has improved and standardized slide quality.
authors.affiliation: Special Hematology, London Health Sciences Centre, London, ON, Canada.
authors.forename: W
authors.initials: W
authors.lastname: Brown
authors.forename: M
authors.initials: M
authors.lastname: Keeney
authors.forename: B D
authors.initials: BD
authors.lastname: Hedley
mesh_terms: D057205:Automation, Laboratory; D001772:Blood Cell Count / Q000295:instrumentation* / Q000379:methods / Q000592:standards*; D006801:Humans; D015203:Reproducibility of Results
keywords: Automated slidemaker/stainer; blood film quality; peripheral blood film; stain quality