title: Genes required for growth at high hydrostatic pressure in Escherichia coli K-12 identified by genome-wide screening.
abstract: Despite the fact that much of the global microbial biosphere is believed to exist in high pressure environments, the effects of hydrostatic pressure on microbial physiology remain poorly understood. We use a genome-wide screening approach, combined with a novel high-throughput high-pressure cell culture method, to investigate the effects of hydrostatic pressure on microbial physiology in vivo. The Keio collection of single-gene deletion mutants in Escherichia coli K-12 was screened for growth at a range of pressures from 0.1 MPa to 60 MPa. This led to the identification of 6 genes, rodZ, holC, priA, dnaT, dedD and tatC, whose products were required for growth at 30 MPa and a further 3 genes, tolB, rffT and iscS, whose products were required for growth at 40 MPa. Our results support the view that the effects of pressure on cell physiology are pleiotropic, with DNA replication, cell division, the cytoskeleton and cell envelope physiology all being potential failure points for cell physiology during growth at elevated pressure.
authors.affiliation: SUPA School of Physics and Astronomy, University of Edinburgh, Edinburgh, Midlothian, United Kingdom ; Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, Midlothian, United Kingdom.
authors.forename: S Lucas
authors.initials: SL
authors.lastname: Black
authors.forename: Angela
authors.initials: A
authors.lastname: Dawson
authors.forename: F Bruce
authors.initials: FB
authors.lastname: Ward
authors.forename: Rosalind J
authors.initials: RJ
authors.lastname: Allen
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D055106:Genome-Wide Association Study; D006874:Hydrostatic Pressure; D009154:Mutation
references.citation: EMBO J. 2009 Feb 4;28(3):171-2
references.pmid: 19194484
references.citation: Nucleic Acids Res. 2004 Dec 02;32(21):6378-87
references.pmid: 15576682
references.citation: Appl Microbiol Biotechnol. 2002 Oct;60(1-2):12-23
references.pmid: 12382038
references.citation: Appl Environ Microbiol. 2004 Mar;70(3):1545-54
references.pmid: 15006777
references.citation: Mol Microbiol. 1991 Aug;5(8):1853-62
references.pmid: 1722555
references.citation: J Biol Chem. 1998 Jul 17;273(29):18003-6
references.pmid: 9660752
references.citation: J Bacteriol. 1964 Mar;87:710-9
references.pmid: 14129671
references.citation: J Bacteriol. 1975 Mar;121(3):753-8
references.pmid: 1090601
references.citation: J Cell Biol. 1975 Jun;65(3):603-14
references.pmid: 1133117
references.citation: Annu Rev Microbiol. 2006;60:373-95
references.pmid: 16756481
references.citation: J Bacteriol. 2002 Feb;184(3):754-9
references.pmid: 11790745
references.citation: Mol Microbiol. 2007 Feb;63(4):1008-25
references.pmid: 17233825
references.citation: Extremophiles. 2005 Feb;9(1):65-73
references.pmid: 15340867
references.citation: Mol Microbiol. 2003 Jun;48(5):1183-93
references.pmid: 12787348
references.citation: Genetics. 2004 Jun;167(2):569-78
references.pmid: 15238512
references.citation: J Bacteriol. 2009 Oct;191(20):6383-93
references.pmid: 19700526
references.citation: J Bacteriol. 1949 Feb;57(2):179-89
references.pmid: 16561663
references.citation: Extremophiles. 1997 Nov;1(4):207-11
references.pmid: 9680302
references.citation: Cell Motil Cytoskeleton. 1988;10(3):380-90
references.pmid: 3052872
references.citation: Mol Syst Biol. 2006;2:2006.0008
references.pmid: 16738554
references.citation: Bibl Laeger. 1966 Mar 14;112(3):573-80
references.pmid: 5330208
references.citation: J Biol Chem. 1990 Aug 15;265(23):13490-7
references.pmid: 2166030
references.citation: FEMS Microbiol Rev. 1988 Sep;4(3):195-222
references.pmid: 3078744
references.citation: J Bacteriol. 1978 Dec;136(3):1008-17
references.pmid: 363684
references.citation: J Bacteriol. 2008 Mar;190(5):1699-709
references.pmid: 18156275
references.citation: Mol Microbiol. 2005 Dec;58(5):1381-91
references.pmid: 16313623
references.citation: Proc Natl Acad Sci U S A. 2009 Jan 27;106(4):1239-44
references.pmid: 19164570
references.citation: J Bacteriol. 1998 Sep;180(18):4872-8
references.pmid: 9733690
references.citation: Appl Environ Microbiol. 2004 May;70(5):2660-6
references.pmid: 15128516
references.citation: J Bacteriol. 1999 Apr;181(8):2330-7
references.pmid: 10197992
references.citation: Proc Natl Acad Sci U S A. 1990 Aug;87(15):5589-93
references.pmid: 2198567
references.citation: Prog Lipid Res. 1990;29(3):167-227
references.pmid: 2131463
references.citation: J Bacteriol. 2009 Dec;191(24):7383-401
references.pmid: 19684127
references.citation: DNA Res. 2005;12(5):291-9
references.pmid: 16769691
references.citation: EMBO J. 2009 Feb 4;28(3):193-204
references.pmid: 19078962
references.citation: FEMS Microbiol Lett. 1999 Aug 15;177(2):191-7
references.pmid: 10474183
references.citation: Biochim Biophys Acta. 2002 Mar 25;1595(1-2):367-81
references.pmid: 11983409
references.citation: Exp Cell Res. 1996 Sep 15;227(2):285-97
references.pmid: 8831567
references.citation: Mol Cell. 2007 Jun 22;26(6):781-93
references.pmid: 17588514
references.citation: J Bacteriol. 1976 Aug;127(2):755-62
references.pmid: 60330
references.citation: FEBS Lett. 1976 Apr 15;64(1):40-3
references.pmid: 773700
references.citation: Proteins. 1996 Jan;24(1):81-91
references.pmid: 8628735
references.citation: Biosci Biotechnol Biochem. 1995 Jan;59(1):130-2
references.pmid: 7765962
references.citation: Nature. 1973 Dec 21-28;246(5434):525-7
references.pmid: 4586891
references.citation: J Bacteriol. 2002 Dec;184(24):6820-9
references.pmid: 12446632
references.citation: J Bacteriol. 1962 Dec;84:1228-36
references.pmid: 14003850
references.citation: Mol Microbiol. 2000 Nov;38(4):904-15
references.pmid: 11115123
references.citation: Chem Soc Rev. 2006 Oct;35(10):908-17
references.pmid: 17003897
references.citation: Biophys J. 2012 Apr 18;102(8):1872-80
references.pmid: 22768943
references.citation: Appl Environ Microbiol. 2008 Oct;74(20):6298-305
references.pmid: 18723648
references.citation: J Bacteriol. 2010 Oct;192(19):4847-58
references.pmid: 20693330
references.citation: J Bacteriol. 1993 Nov;175(22):7170-7
references.pmid: 8226663
references.citation: Microbiology (Reading). 2004 Jun;150(Pt 6):1965-1972
references.pmid: 15184582
references.citation: Mol Microbiol. 2003 Jun;48(5):1171-82
references.pmid: 12787347
references.citation: Proc Natl Acad Sci U S A. 1986 Mar;83(5):1256-60
references.pmid: 3006041
references.citation: Rev Sci Instrum. 2010 May;81(5):053710
references.pmid: 20515148
references.citation: J Bacteriol. 2005 May;187(10):3359-68
references.pmid: 15866920
references.citation: FEBS Lett. 1990 Jul 16;267(2):239-41
references.pmid: 2199216
references.citation: J Bacteriol. 1998 Nov;180(22):5875-84
references.pmid: 9811644
references.citation: Annu Rev Phys Chem. 1993;44:89-113
references.pmid: 8257561
references.citation: EMBO J. 1998 Apr 15;17(8):2436-49
references.pmid: 9545254
references.citation: J Bacteriol. 2010 Jan;192(1):242-55
references.pmid: 19880599
references.citation: J Bacteriol. 2011 Sep;193(18):4973-83
references.pmid: 21764913
references.citation: Trends Biotechnol. 2009 Jul;27(7):434-41
references.pmid: 19497630
references.citation: Microbiology (Reading). 2005 Oct;151(Pt 10):3337-3347
references.pmid: 16207916
references.citation: J Bacteriol. 2000 Sep;182(17):4764-72
references.pmid: 10940016
references.citation: EMBO J. 2008 Dec 3;27(23):3081-91
references.pmid: 19008860
references.citation: Biophys J. 1969 Dec;9(12):1464-82
references.pmid: 4900824
references.citation: Nucleic Acids Res. 2003 Aug 1;31(15):4434-40
references.pmid: 12888503
references.citation: Annu Rev Biophys Bioeng. 1982;11:1-21
references.pmid: 7049058
references.citation: Appl Environ Microbiol. 2005 May;71(5):2226-31
references.pmid: 15870304
references.citation: Genetics. 2008 Feb;178(2):851-72
references.pmid: 18245339
references.citation: J Biol Chem. 1992 Jan 15;267(2):746-53
references.pmid: 1730666
references.citation: J Bacteriol. 2004 Sep;186(18):6133-41
references.pmid: 15342583
references.citation: J Bacteriol. 2001 Jan;183(1):139-44
references.pmid: 11114910