title: Multiplex ligation-dependent probe amplification (MLPA)--new possibilities of prenatal diagnosis.
abstract: Multiplex Ligation-dependent Probe Amplification (MLPA) is a relatively new method of molecular diagnosis. It enables a relative quantitative assessment of up to 50 different PCR amplicons in one reaction by the use of a very small amount of examined DNA. Nowadays MLPA is becoming a very helpful tool in prenatal diagnosis and is widely used for the detection of aneuploidies, familial single gene disorders, common microdeletion syndromes, sub-telomeric alterations and identification of marker chromosomes in fetuses. This review demonstrates possible applications of MLPA in prenatal diagnosis.
authors.affiliation: Department of Obstetrics and Gynecology of the Postgraduate Center of Medical Education, Warsaw, Poland. diana_massalska@wp.pl
authors.forename: Diana
authors.initials: D
authors.lastname: Massalska
authors.forename: Julia
authors.initials: J
authors.lastname: Bijok
authors.forename: Janusz G
authors.initials: JG
authors.lastname: Zimowski
authors.forename: Anna
authors.initials: A
authors.lastname: Jóźwiak
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Jakiel
authors.forename: Tomasz
authors.initials: T
authors.lastname: Roszkowski
mesh_terms: D025063:Chromosome Disorders / Q000175:diagnosis*; D005260:Female; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D011295:Prenatal Care / Q000379:methods; D011296:Prenatal Diagnosis / Q000379:methods*