title: Knockdown of maternal homeobox transcription factor SEBOX gene impaired early embryonic development in porcine parthenotes.
abstract: A number of germ cell-specific transcription factors essential for ovarian formation and folliculogenesis have been identified and studied. However, the role of these factors during early embryonic development has been poorly explored. In the present study, we investigated the role of SEBOX, a maternal homeobox transcription factor, during early embryonic development in porcine parthenotes. mRNA for SEBOX is preferentially expressed in oocytes, and expression persists until embryonic genome activation (EGA). Knockdown of SEBOX by siRNA disrupted early embryonic development, but not oocyte maturation. Many maternal genes essential for early embryonic development were upregulated in SEBOX-depleted embryos. Moreover, some pluripotency-associated genes, including SOX2 and NANOG, were upregulated when SEBOX was knocked down. Therefore, our data demonstrate that SEBOX is required for early embryonic development in pigs and appears to regulate the degradation of maternal transcripts and the expression of pluripotency genes.
authors.affiliation: Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zheng
authors.forename: Ming-Hui
authors.initials: MH
authors.lastname: Zhao
authors.forename: Jia-Lin
authors.initials: JL
authors.lastname: Jia
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Heo
authors.forename: Xiang-Shun
authors.initials: XS
authors.lastname: Cui
authors.forename: Jeong Su
authors.initials: JS
authors.lastname: Oh
authors.forename: Nam-Hyung
authors.initials: NH
authors.lastname: Kim
mesh_terms: D000003:Abattoirs; D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D054885:Cumulus Cells / Q000166:cytology / Q000378:metabolism; D004477:Ectogenesis; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006107:Granulosa Cells / Q000166:cytology / Q000378:metabolism; D018398:Homeodomain Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D059471:In Vitro Oocyte Maturation Techniques; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D010312:Parthenogenesis; D034622:RNA Interference; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D034421:Sus scrofa; D015854:Up-Regulation
chemical_list: D018398:Homeodomain Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D055748:SOXB1 Transcription Factors
references.citation: Nat Genet. 2000 Nov;26(3):267-8
references.pmid: 11062459
references.citation: Genes Dev. 2011 Apr 1;25(7):755-66
references.pmid: 21460039
references.citation: Biol Reprod. 1991 Jan;44(1):62-8
references.pmid: 2015352
references.citation: Development. 2010 Mar;137(6):859-70
references.pmid: 20179092
references.citation: Biol Reprod. 2008 Dec;79(6):1192-201
references.pmid: 18753614
references.citation: Development. 1998 Jan;125(2):237-48
references.pmid: 9486797
references.citation: Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8904-9
references.pmid: 10922053
references.citation: Hum Reprod Update. 2002 Jul-Aug;8(4):323-31
references.pmid: 12206467
references.citation: Mol Biol Cell. 2008 Oct;19(10):4383-92
references.pmid: 18701707
references.citation: Genes Dev. 2006 Jul 1;20(13):1744-54
references.pmid: 16818606
references.citation: Endocrinology. 2011 Mar;152(3):1013-23
references.pmid: 21193554
references.citation: Proc Natl Acad Sci U S A. 2006 May 23;103(21):8090-5
references.pmid: 16690745
references.citation: Dev Cell. 2004 Jan;6(1):117-31
references.pmid: 14723852
references.citation: Science. 2004 Aug 20;305(5687):1157-9
references.pmid: 15326356
references.citation: Nat Genet. 2003 Feb;33(2):187-91
references.pmid: 12539046
references.citation: BMC Dev Biol. 2011 May 06;11:25
references.pmid: 21548929
references.citation: Curr Biol. 2003 Dec 2;13(23):2110-7
references.pmid: 14654002
references.citation: Biol Reprod. 2008 Sep;79(3):442-9
references.pmid: 18509161
references.citation: Zygote. 2014 Feb;22(1):64-8
references.pmid: 22805237
references.citation: Science. 2003 Apr 25;300(5619):633-6
references.pmid: 12714744
references.citation: Cell. 1992 Jan 24;68(2):283-302
references.pmid: 1346368
references.citation: Development. 2009 Sep;136(18):3033-42
references.pmid: 19700615