title: Complete mitochondrial genome of the two-spotted stag beetle, Metopodontus blanchardi (Coleoptera: Lucanidae).
abstract: We have completely sequenced the mitochondrial genome (mitogeome) of the two-spotted stag beetle, Metopodontus blanchardi, which is listed as a first-degree endangered species in Korea. The complete mitogenome of M. blanchardi was determined to be 21,628 bp, indicating at least 5 kb larger in size than typical animal mitogenomes. Such a long M. blanchardi genome stems from a 3100-bp long A + T-rich region and a 4051-bp long, large non-coding sequence located between tRNA(Ile) and tRNA(Gln). The A + T-rich region is composed of duplicated repeat sequences (each 965 bp and 969 bp), and three non-repeat sequences encompassing the repeat sequences. The 4051-bp long non-coding sequence is composed of ∼ 17 tandem repeat sequences, each of which is composed of two subunits (113-bp and 104-bp long subunits) and this is encompassed by non-repeat sequences. The start codon for COI gene of M. blanchardi was designated as unconventional AAG (Lysine) by following a previous study.
authors.affiliation: College of Agriculture & Life Sciences, Chonnam National University , Gwangju , Republic of Korea .
authors.forename: Min Jee
authors.initials: MJ
authors.lastname: Kim
authors.forename: Ki-Gyoung
authors.initials: KG
authors.lastname: Kim
authors.forename: Seong Ryul
authors.initials: SR
authors.lastname: Kim
authors.forename: Iksoo
authors.initials: I
authors.lastname: Kim
mesh_terms: D000818:Animals; D001482:Base Composition; D001517:Coleoptera / Q000235:genetics*; D050259:Genes, Mitochondrial; D054629:Genome, Mitochondrial; D016366:Open Reading Frames
keywords: A + T-rich region; Metopodontus blanchardi; mitochondrial genome; tandem repeat sequence