title: In-hive patterns of temporal polyethism in strains of honey bees (Apis mellifera) with distinct genetic backgrounds.
abstract: Honey bee workers exhibit an age-based division of labor (temporal polyethism, DOL). Younger bees transition through sets of tasks within the nest; older bees forage outside. Components of temporal polyethism remain unrevealed. Here, we investigate the timing and pattern of pre-foraging behavior in distinct strains of bees to (1) determine if a general pattern of temporal DOL exists in honey bees, (2) to demonstrate a direct genetic impact on temporal pacing, and (3) to further elucidate the mechanisms controlling foraging initiation. Honey bees selected for differences in stored pollen demonstrate consistent differences in foraging initiation age. Those selected for increased pollen storage (high pollen hoarding strain, HSBs) initiate foraging earlier in life than those selected for decreased pollen storage (low pollen hoarding strain, LSBs). We found that HSBs both initiate and terminate individual pre-foraging tasks earlier than LSBs when housed in a common hive environment. Unselected commercial bees (wild type) generally demonstrated intermediate behavioral timing. There were few differences between genotypes for the proportion of pre-foraging effort dedicated to individual tasks, though total pre-foraging effort differences differed dramatically. This demonstrates that behavioral pacing can be accelerated or slowed, but the pattern of behavior is not fundamentally altered, suggesting a general pattern of temporal behavior in honey bees. This also demonstrates direct genetic control of temporal pacing. Finally, our results suggest that earlier HSB protein (pollen) consumption termination compared to LSBs may contribute to an earlier decline in hemolymph vitellogenin protein titers, which would explain their earlier onset of foraging.
authors.affiliation: School of Life Sciences, Arizona State University, Tempe, AZ USA ; Department of Ecology, Evolution and Behavior, Alexander Silberman Institute of Life Sciences, The Hebrew University, Jerusalem, Israel.
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Siegel
authors.forename: M Kim
authors.initials: MK
authors.lastname: Fondrk
authors.forename: Gro V
authors.initials: GV
authors.lastname: Amdam
authors.forename: Robert E
authors.initials: RE
authors.lastname: Page
keywords: Behavioral pacing; Behavioral pattern; Division of labor; Honey bee worker; Pre-foraging behavior; Temporal polyethism
references.citation: Nature. 2006 Jan 5;439(7072):76-8
references.pmid: 16397498
references.citation: Evol Dev. 2010 Sep-Oct;12(5):428-36
references.pmid: 20883212
references.citation: J Comp Physiol A. 1998 Apr;182(4):489-500
references.pmid: 9565478
references.citation: Am Nat. 2007 Jul;170(1):37-46
references.pmid: 17853990
references.citation: Curr Top Dev Biol. 2006;74:253-86
references.pmid: 16860670
references.citation: Monogr Popul Biol. 1978;12:1-352
references.pmid: 740003
references.citation: Proc Biol Sci. 2003 Jan 22;270(1511):147-52
references.pmid: 12590752
references.citation: Q Rev Biol. 2005 Sep;80(3):317-36
references.pmid: 16250466
references.citation: Am Nat. 2009 Mar;173(3):E99-E107
references.pmid: 19140771
references.citation: J Comp Physiol A. 1999 Aug;185(2):207-13
references.pmid: 10488557
references.citation: Trends Ecol Evol. 1992 Oct;7(10):346-9
references.pmid: 21236060
references.citation: PLoS Biol. 2007 Mar;5(3):e62
references.pmid: 17341131
references.citation: Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11726-9
references.pmid: 1465390
references.citation: Behav Ecol Sociobiol. 2010 Jan;64(3):305-316
references.pmid: 20119486
references.citation: J Insect Physiol. 1974 May;20(5):897-909
references.pmid: 4209093
references.citation: Proc Natl Acad Sci U S A. 2003 Nov 25;100 Suppl 2:14519-25
references.pmid: 14573707
references.citation: Proc Natl Acad Sci U S A. 2004 Aug 3;101(31):11350-5
references.pmid: 15277665
references.citation: J Hered. 2000 Nov-Dec;91(6):474-9
references.pmid: 11218085
references.citation: J Insect Physiol. 1998 Oct;44(10):929-939
references.pmid: 12770429
references.citation: Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1799-802
references.pmid: 12566563