title: Mammaglobin and S-100 immunoreactivity in salivary gland carcinomas other than mammary analogue secretory carcinoma.
abstract: Mammary analogue secretory carcinoma (MASC) is a recently described salivary gland tumor that has morphologic features similar to secretory carcinoma of the breast and that also harbors the same ETV6 translocation. Diffuse mammaglobin and S-100 immunoreactivity are used to differentiate MASC from its morphologic mimics, especially acinic cell carcinoma and adenocarcinoma, not otherwise specified. However, the combination of mammaglobin and S-100 immunoreactivity has not been well studied in other types of salivary gland carcinomas that may have focal areas reminiscent of MASC. Here we evaluated mammaglobin and S-100 immunoreactivity in 15 cases each of polymorphous low-grade adenocarcinoma, adenoid cystic carcinoma and mucoepidermoid carcinoma, and also in 2 cases of adenocarcinoma, not otherwise specified, and 1 mucinous adenocarcinoma. Cases with significant co-expression of mammaglobin and S-100 (moderate or strong immunoreactivity in >25% of tumor cells) were further analyzed by fluorescence in situ hybridization using the ETV6 (12p13) break-apart probe. Nine cases (60%) of polymorphous low-grade adenocarcinoma and two (13.3%) of adenoid cystic carcinoma met the criteria for significant co-expression of mammaglobin and S-100. All were negative for the ETV6 translocation by fluorescence in situ hybridization. Although mammaglobin and S-100 positivity was seen in the majority of polymorphous low-grade adenocarcinomas and a minority of adenoid cystic carcinomas, none were positive for the ETV6 translocation characteristic of MASC. This indicates a need for caution in the use of immunohistochemistry for diagnosing MASC, especially in the absence of cytogenetic confirmation.
authors.affiliation: Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA.
authors.forename: Kalyani R
authors.initials: KR
authors.lastname: Patel
authors.forename: Isaac H
authors.initials: IH
authors.lastname: Solomon
authors.forename: Samir K
authors.initials: SK
authors.lastname: El-Mofty
authors.forename: James S
authors.initials: JS
authors.lastname: Lewis
authors.forename: Rebecca D
authors.initials: RD
authors.lastname: Chernock
mesh_terms: D000230:Adenocarcinoma / Q000145:classification / Q000378:metabolism* / Q000473:pathology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001706:Biopsy; D001943:Breast Neoplasms / Q000145:classification / Q000378:metabolism* / Q000473:pathology; D002277:Carcinoma / Q000145:classification / Q000378:metabolism* / Q000473:pathology; D003528:Carcinoma, Adenoid Cystic / Q000145:classification / Q000378:metabolism / Q000473:pathology; D018277:Carcinoma, Mucoepidermoid / Q000145:classification / Q000378:metabolism / Q000473:pathology; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D060785:Mammaglobin A / Q000378:metabolism*; D008875:Middle Aged; D050783:Proto-Oncogene Proteins c-ets / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D009418:S100 Proteins / Q000378:metabolism*; D012468:Salivary Gland Neoplasms / Q000145:classification / Q000378:metabolism* / Q000473:pathology; D014178:Translocation, Genetic; D055815:Young Adult; D000097764:ETS Translocation Variant 6 Protein
chemical_list: D014408:Biomarkers, Tumor; D060785:Mammaglobin A; D050783:Proto-Oncogene Proteins c-ets; D012097:Repressor Proteins; D009418:S100 Proteins; D000097764:ETS Translocation Variant 6 Protein
keywords: Adenoid cystic carcinoma; Mammaglobin; Mammary analogue secretory carcinoma; Mucoepidermoid carcinoma; Polymorphous low-grade adenocarcinoma; S-100