title: DNA detection by cascade enzymatic signal amplification.
abstract: Although many approaches based on template replication were developed and applied in DNA detection, cross-contamination from amplicons is always a vexing problem. Thus, signal amplification is preferable for DNA detection due to its low risk of cross-contamination from amplicons. Here, we proposed a cascade enzymatic signal amplification (termed as CESA) by coupling Afu flap endonuclease with nicking endonuclease, including three steps: invasive signal amplification, flap ligation, and nicking endonuclease signal amplification. Because of the advantages of low risk of contamination, no sequence requirement of target DNA, and the universal reaction conditions for any target detection, CESA has a great potential in clinical diagnosis.
authors.affiliation: Department of Pharmacology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China.
authors.forename: Bingjie
authors.initials: B
authors.lastname: Zou
authors.forename: Yinjiao
authors.initials: Y
authors.lastname: Ma
authors.forename: Guohua
authors.initials: G
authors.lastname: Zhou
mesh_terms: D055162:Biocatalysis; D015374:Biosensing Techniques; D053904:DNA Breaks, Single-Stranded; D053837:DNA Cleavage; D059018:DNA Contamination; D015342:DNA Probes / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry; D045585:Flap Endonucleases / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D059629:Signal-To-Noise Ratio; D013050:Spectrometry, Fluorescence
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes; D045585:Flap Endonucleases; C053864:endodeoxyribonuclease BsmI; D015252:Deoxyribonucleases, Type II Site-Specific