title: White spot syndrome virus IE1 and WSV056 modulate the G1/S transition by binding to the host retinoblastoma protein.
abstract: DNA viruses often target cellular proteins to modulate host cell cycles and facilitate viral genome replication. However, whether proliferation of white spot syndrome virus (WSSV) requires regulation of the host cell cycle remains unclear. In the present study, we show that two WSSV paralogs, IE1 and WSV056, can interact with Litopenaeus vannamei retinoblastoma (Rb)-like protein (lv-RBL) through the conserved LxCxE motif. Further investigation revealed that IE1 and WSV056 could also bind to Drosophila retinoblastoma family protein 1 (RBF1) in a manner similar to how they bind to lv-RBL. Using the Drosophila RBF-E2F pathway as a model system, we demonstrated that both IE1 and WSV056 could sequester RBF1 from Drosophila E2F transcription factor 1 (E2F1) and subsequently activate E2F1 to stimulate the G1/S transition. Our findings provide the first evidence that WSSV may regulate cell cycle progression by targeting the Rb-E2F pathway.
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration, Third Institute of Oceanography, Xiamen, People's Republic of China.
authors.forename: Xiaozhuo
authors.initials: X
authors.lastname: Ran
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Bian
authors.forename: Yongchang
authors.initials: Y
authors.lastname: Ji
authors.forename: Xiumin
authors.initials: X
authors.lastname: Yan
authors.forename: Feng
authors.initials: F
authors.lastname: Yang
authors.forename: Fang
authors.initials: F
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002453:Cell Cycle; D004266:DNA Virus Infections / Q000235:genetics / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D016193:G1 Phase; D054884:Host-Pathogen Interactions; D017874:Immediate-Early Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000166:cytology / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D011485:Protein Binding; D016160:Retinoblastoma Protein / Q000235:genetics / Q000378:metabolism*; D016196:S Phase; D016415:Sequence Alignment; D046848:White spot syndrome virus 1 / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*
chemical_list: D017874:Immediate-Early Proteins; D016160:Retinoblastoma Protein
references.citation: Curr Top Microbiol Immunol. 2009;328:197-227
references.pmid: 19216439
references.citation: J Biol Chem. 1995 Oct 20;270(42):25159-65
references.pmid: 7559650
references.citation: J Virol. 2007 Feb;81(3):1461-71
references.pmid: 17079306
references.citation: J Gen Virol. 2004 Jul;85(Pt 7):2035-2044
references.pmid: 15218189
references.citation: J Virol. 2003 Jan;77(2):851-61
references.pmid: 12502801
references.citation: Oncogene. 2006 Aug 28;25(38):5277-85
references.pmid: 16936748
references.citation: EMBO J. 2002 Sep 16;21(18):4927-37
references.pmid: 12234932
references.citation: J Virol. 2011 Jul;85(13):6535-47
references.pmid: 21507980
references.citation: Cell Div. 2009 Jan 15;4:1
references.pmid: 19146698
references.citation: Mol Cell Biol. 2000 Sep;20(18):6799-805
references.pmid: 10958676
references.citation: Virus Res. 2005 Jun;110(1-2):21-30
references.pmid: 15845252
references.citation: Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10683-8
references.pmid: 12145325
references.citation: Dev Comp Immunol. 2008;32(10):1142-50
references.pmid: 18460415
references.citation: Virology. 2000 Nov 10;277(1):100-10
references.pmid: 11062040
references.citation: Oncogene. 2005 Nov 21;24(52):7673-85
references.pmid: 16299528
references.citation: Front Biosci. 2008 Jan 01;13:1003-17
references.pmid: 17981607
references.citation: Virology. 2005 Jul 5;337(2):399-406
references.pmid: 15913698
references.citation: J Virol. 2001 Dec;75(23):11811-20
references.pmid: 11689662
references.citation: Methods Mol Biol. 2005;292:231-44
references.pmid: 15507712
references.citation: J Virol. 2012 Apr;86(8):4294-304
references.pmid: 22301153
references.citation: Nat Rev Mol Cell Biol. 2008 Sep;9(9):713-24
references.pmid: 18719710
references.citation: Curr Opin Immunol. 2009 Feb;21(1):3-9
references.pmid: 19223163
references.citation: J Gen Virol. 2000 Feb;81(Pt 2):307-16
references.pmid: 10644828
references.citation: J Virol. 2008 Nov;82(22):11362-73
references.pmid: 18768963
references.citation: Cell. 2010 Feb 5;140(3):436-436.e2
references.pmid: 20144765
references.citation: Virology. 2001 Jul 20;286(1):7-22
references.pmid: 11448154
references.citation: Virus Res. 2007 May;125(2):145-52
references.pmid: 17240469
references.citation: Antioxid Redox Signal. 2012 Sep 15;17(6):914-26
references.pmid: 22332765
references.citation: Virology. 2010 Oct 25;406(2):176-80
references.pmid: 20684968
references.citation: J Virol. 2001 May;75(10):4475-81
references.pmid: 11312317
references.citation: Oncogene. 2006 Aug 28;25(38):5286-93
references.pmid: 16936749
references.citation: Virology. 2009 Mar 1;385(1):267-74
references.pmid: 19135226
references.citation: Virology. 2002 Sep 15;301(1):136-47
references.pmid: 12359454
references.citation: PLoS Pathog. 2007 Sep 7;3(9):1335-47
references.pmid: 17907805
references.citation: Proc Natl Acad Sci U S A. 2005 Dec 13;102(50):18159-64
references.pmid: 16332962
references.citation: PLoS One. 2011;6(9):e24773
references.pmid: 21931849
references.citation: Virology. 2005 Apr 10;334(2):327-41
references.pmid: 15780883
references.citation: Arch Virol. 2011 Sep;156(9):1611-4
references.pmid: 21544545