title: Saikosaponin a and saikosaponin d inhibit proliferation and migratory activity of rat HSC-T6 cells.
abstract: The proliferation and migration of hepatic stellate cells (HSCs) profoundly impact the pathogenesis of liver inflammation and fibrogenesis. As a perennial herb native to China, Bupleurum falcatum is administered for its anti-inflammatory, antipyretic, and antihepatotoxic effects. Saikosaponin a (SSa) and Saikosaponin d (SSd) are the major active components of triterpene saponins in Bupleurum falcatum. This study analyzes how SSa and SSd affect rat HSC-T6 cell line proliferation and migration. Experimental results indicate that, in addition to suppressing HSC-T6 proliferation, wound healing activity and cell migration in a time- and dose-dependent manner, SSa and SSd significantly induce apoptosis. Additionally, SSa and SSd decreased the expressions of extracellular matrix-regulated kinase 1/2 (ERK1/2), platelet-derived growth factor receptor 1 (PDGFR1), and subsequently transforming growth factor-β1 receptor (TGF-β1R), α-smooth muscle actin, TGF-β1 and connective tissue growth factor. They also decreased phosphorylation of p38 (p-p38) and ERK1/2 (p-ERK1/2) of HSC-T6. Furthermore, both SSa and SSd can block PDGF-BB and TGF-β1-induced cell proliferation and migration of HSC-T6. These results suggest that SSa and SSd may inhibit proliferation and activation of HSC-T6, and the modulated mechanisms warrant further study.
authors.affiliation: 1 Department of Gastroenterology and Hepatology, E-DA hospital , Kaohsiung, Taiwan .
authors.forename: Ming Feng
authors.initials: MF
authors.lastname: Chen
authors.forename: Chao Cheng
authors.initials: CC
authors.lastname: Huang
authors.forename: Pei Shan
authors.initials: PS
authors.lastname: Liu
authors.forename: Chang Han
authors.initials: CH
authors.lastname: Chen
authors.forename: Li Yen
authors.initials: LY
authors.lastname: Shiu
mesh_terms: D000818:Animals; D000077214:Becaplermin; D029972:Bupleurum / Q000737:chemistry*; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006131:Growth Inhibitors / Q000494:pharmacology*; D055166:Hepatic Stellate Cells / Q000166:cytology / Q000187:drug effects*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D020574:Proto-Oncogene Proteins c-sis / Q000235:genetics / Q000378:metabolism; D051381:Rats; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D012503:Saponins / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D006131:Growth Inhibitors; D020574:Proto-Oncogene Proteins c-sis; D018125:Receptors, Transforming Growth Factor beta; D012503:Saponins; D053773:Transforming Growth Factor beta1; D000077214:Becaplermin; D009828:Oleanolic Acid; C025759:saikosaponin D
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