title: Modulation of taste responsiveness by the satiation hormone peptide YY.
abstract: It has been hypothesized that the peripheral taste system may be modulated in the context of an animal's metabolic state. One purported mechanism for this phenomenon is that circulating gastrointestinal peptides modulate the functioning of the peripheral gustatory system. Recent evidence suggests endocrine signaling in the oral cavity can influence food intake (FI) and satiety. We hypothesized that these hormones may be affecting FI by influencing taste perception. We used immunohistochemistry along with genetic knockout models and the specific reconstitution of peptide YY (PYY) in saliva using gene therapy protocols to identify a role for PYY signaling in taste. We show that PYY is expressed in subsets of taste cells in murine taste buds. We also show, using brief-access testing with PYY knockouts, that PYY signaling modulates responsiveness to bitter-tasting stimuli, as well as to lipid emulsions. We show that salivary PYY augmentation, via viral vector therapy, rescues behavioral responsiveness to a lipid emulsion but not to bitter stimuli and that this response is likely mediated via activation of Y2 receptors localized apically in taste cells. Our findings suggest distinct functions for PYY produced locally in taste cells vs. that circulating systemically.
authors.affiliation: 1Department of Neuroscience, University of Florida McKnight Brain Institute, 1149 Newell Dr., Box 100244, Gainesville, FL 32611, USA. c.dotson@mbi.ufl.edu.
authors.forename: Michael S
authors.initials: MS
authors.lastname: La Sala
authors.forename: Maria D
authors.initials: MD
authors.lastname: Hurtado
authors.forename: Alicia R
authors.initials: AR
authors.lastname: Brown
authors.forename: Diego V
authors.initials: DV
authors.lastname: Bohórquez
authors.forename: Rodger A
authors.initials: RA
authors.lastname: Liddle
authors.forename: Herbert
authors.initials: H
authors.lastname: Herzog
authors.forename: Sergei
authors.initials: S
authors.lastname: Zolotukhin
authors.forename: Cedrick D
authors.initials: CD
authors.lastname: Dotson
mesh_terms: D000818:Animals; D004435:Eating; D051379:Mice; D018345:Mice, Knockout; D019894:Peptide YY / Q000235:genetics / Q000378:metabolism*; D017476:Receptors, Neuropeptide Y / Q000235:genetics / Q000378:metabolism; D012528:Satiety Response; D013649:Taste; D013650:Taste Buds / Q000378:metabolism
chemical_list: D017476:Receptors, Neuropeptide Y; C095889:neuropeptide Y2 receptor; D019894:Peptide YY
keywords: behavior; endocrine signaling; fat intake; gustation; hormonal signaling
references.citation: Physiol Behav. 2005 Aug 7;85(5):655-61
references.pmid: 16083923
references.citation: Neurogastroenterol Motil. 1997 Jun;9(2):109-16
references.pmid: 9198086
references.citation: PLoS One. 2011;6(10):e26137
references.pmid: 22028819
references.citation: Chem Senses. 2004 Jul;29(6):489-98
references.pmid: 15269121
references.citation: Histochem Cell Biol. 1999 Jul;112(1):79-86
references.pmid: 10461815
references.citation: Diabetes. 2008 Oct;57(10):2661-5
references.pmid: 18633111
references.citation: Curr Opin Endocrinol Diabetes Obes. 2011 Feb;18(1):56-60
references.pmid: 21157324
references.citation: Brain Res. 2001 Jan 26;890(1):32-7
references.pmid: 11164766
references.citation: J Neurochem. 2008 Jul;106(1):455-63
references.pmid: 18397368
references.citation: Appetite. 2001 Feb;36(1):93-8
references.pmid: 11161349
references.citation: Endocrinology. 2004 Feb;145(2):839-47
references.pmid: 14592964
references.citation: PLoS One. 2012;7(9):e46358
references.pmid: 23050020
references.citation: Acta Histochem. 2011 Oct;113(6):663-7
references.pmid: 20926122
references.citation: Br J Nutr. 2010 Jul;104(1):145-52
references.pmid: 20196892
references.citation: Pharmacol Rev. 1998 Mar;50(1):143-50
references.pmid: 9549761
references.citation: Pharmacol Ther. 2011 Jul;131(1):91-113
references.pmid: 21439311
references.citation: Brain Res. 2010 Apr 14;1325:53-62
references.pmid: 20156422
references.citation: Nutrition. 2008 Sep;24(9):878-84
references.pmid: 18662857
references.citation: Am J Physiol Gastrointest Liver Physiol. 2010 Oct;299(4):G967-79
references.pmid: 20634436
references.citation: Physiol Behav. 2012 Mar 20;105(5):1242-9
references.pmid: 22251965
references.citation: Tohoku J Exp Med. 2010 Apr;220(4):267-71
references.pmid: 20383037
references.citation: Prog Lipid Res. 2011 Jul;50(3):225-33
references.pmid: 21356242
references.citation: Ann Surg. 2006 Nov;244(5):715-22
references.pmid: 17060764
references.citation: Biol Chem. 2013 Aug;394(8):921-36
references.pmid: 23449522
references.citation: Curr Med Chem. 2011;18(22):3417-22
references.pmid: 21728958
references.citation: Diabetologia. 2006 Jun;49(6):1360-70
references.pmid: 16680491
references.citation: Chem Senses. 2005 Sep;30(7):593-600
references.pmid: 16135743
references.citation: Curr Top Med Chem. 2007;7(17):1710-20
references.pmid: 17979780
references.citation: Naunyn Schmiedebergs Arch Pharmacol. 2008 May;377(3):205-7
references.pmid: 18398599
references.citation: Peptides. 2002 Feb;23(2):251-61
references.pmid: 11825640
references.citation: Physiol Behav. 2011 Oct 24;104(5):709-21
references.pmid: 21827777
references.citation: Histochemistry. 1988;89(2):151-61
references.pmid: 2456278
references.citation: Surg Endosc. 2011 Apr;25(4):1176-81
references.pmid: 20844896
references.citation: Am J Physiol Regul Integr Comp Physiol. 2011 Nov;301(5):R1267-80
references.pmid: 21849633
references.citation: Ann N Y Acad Sci. 1998 Nov 30;855:165-8
references.pmid: 9929599
references.citation: Ann N Y Acad Sci. 2009 Jul;1170:98-101
references.pmid: 19686117
references.citation: Physiol Behav. 2007 Mar 16;90(4):602-11
references.pmid: 17210165
references.citation: J Comp Neurol. 2009 Nov 1;517(1):1-14
references.pmid: 19708028
references.citation: Nature. 2002 Aug 8;418(6898):650-4
references.pmid: 12167864
references.citation: Am J Physiol Regul Integr Comp Physiol. 2011 Oct;301(4):R1057-66
references.pmid: 21734019
references.citation: N Engl J Med. 2003 Sep 4;349(10):941-8
references.pmid: 12954742
references.citation: FASEB J. 2010 Oct;24(10):3960-9
references.pmid: 20547661
references.citation: Proc Natl Acad Sci U S A. 2000 Sep 26;97(20):11044-9
references.pmid: 10995460
references.citation: Endocr J. 2010;57(6):467-75
references.pmid: 20431269
references.citation: Physiol Behav. 2008 Oct 20;95(3):476-83
references.pmid: 18691606
references.citation: Methods. 2002 Oct;28(2):158-67
references.pmid: 12413414
references.citation: Curr Opin Gastroenterol. 2010 Mar;26(2):140-5
references.pmid: 19901832
references.citation: Am J Physiol Regul Integr Comp Physiol. 2001 Aug;281(2):R596-606
references.pmid: 11448865
references.citation: Chem Senses. 2003 Oct;28(8):695-704
references.pmid: 14627538
references.citation: J Mol Histol. 2011 Feb;42(1):3-13
references.pmid: 21061049
references.citation: Gene Ther. 2006 Apr;13(7):594-601
references.pmid: 16341060
references.citation: Peptides. 1991 Nov-Dec;12(6):1251-60
references.pmid: 1667688
references.citation: J Comp Neurol. 2007 Jun 20;502(6):1003-11
references.pmid: 17447253
references.citation: J Pharmacol Sci. 2010;112(1):8-12
references.pmid: 20093782