title: Iodine-131 dose-dependent gene expression: alterations in both normal and tumour thyroid tissues of post-Chernobyl thyroid cancers.
abstract: BACKGROUND
  A strong, consistent association between childhood irradiation and subsequent thyroid cancer provides an excellent model for studying radiation carcinogenesis.
  
  
  METHODS
  We evaluated gene expression in 63 paired RNA specimens from frozen normal and tumour thyroid tissues with individual iodine-131 (I-131) doses (0.008-8.6 Gy, no unirradiated controls) received from Chernobyl fallout during childhood (Ukrainian-American cohort). Approximately half of these randomly selected samples (32 tumour/normal tissue RNA specimens) were hybridised on 64 whole-genome microarrays (Agilent, 4 × 44 K). Associations between I-131 dose and gene expression were assessed separately in normal and tumour tissues using Kruskal-Wallis and linear trend tests. Of 155 genes significantly associated with I-131 after Bonferroni correction and with ≥2-fold increase per dose category, we selected 95 genes. On the remaining 31 RNA samples these genes were used for validation purposes using qRT-PCR.
  
  
  RESULTS
  Expression of eight genes (ABCC3, C1orf9, C6orf62, FGFR1OP2, HEY2, NDOR1, STAT3, and UCP3) in normal tissue and six genes (ANKRD46, CD47, HNRNPH1, NDOR1, SCEL, and SERPINA1) in tumour tissue was significantly associated with I-131. PANTHER/DAVID pathway analyses demonstrated significant over-representation of genes coding for nucleic acid binding in normal and tumour tissues, and for p53, EGF, and FGF signalling pathways in tumour tissue.
  
  
  CONCLUSIONS
  The multistep process of radiation carcinogenesis begins in histologically normal thyroid tissue and may involve dose-dependent gene expression changes.
authors.affiliation: Bundeswehr Institute of Radiobiology, Neuherbergstr. 11, 80937 Munich, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Abend
authors.forename: R M
authors.initials: RM
authors.lastname: Pfeiffer
authors.forename: C
authors.initials: C
authors.lastname: Ruf
authors.forename: M
authors.initials: M
authors.lastname: Hatch
authors.forename: T I
authors.initials: TI
authors.lastname: Bogdanova
authors.forename: M D
authors.initials: MD
authors.lastname: Tronko
authors.forename: J
authors.initials: J
authors.lastname: Hartmann
authors.forename: V
authors.initials: V
authors.lastname: Meineke
authors.forename: K
authors.initials: K
authors.lastname: Mabuchi
authors.forename: A V
authors.initials: AV
authors.lastname: Brenner
mesh_terms: D000293:Adolescent; D000328:Adult; D047852:Chernobyl Nuclear Accident; D002648:Child; D005260:Female; D015870:Gene Expression / Q000528:radiation effects*; D055106:Genome-Wide Association Study; D006801:Humans; D007457:Iodine Radioisotopes / Q000008:administration & dosage*; D008297:Male; D009381:Neoplasms, Radiation-Induced / Q000209:etiology* / Q000235:genetics*; D013961:Thyroid Gland / Q000528:radiation effects*; D013964:Thyroid Neoplasms / Q000209:etiology* / Q000235:genetics*; D059467:Transcriptome / Q000528:radiation effects; D055815:Young Adult
chemical_list: D007457:Iodine Radioisotopes
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