title: In vitro suppression of immune responses using monocyte-derived tolerogenic dendritic cells from patients with primary Sjögren's syndrome.
abstract: BACKGROUND
  Therapeutic vaccination with antigen-specific tolerogenic dendritic cells (tolDC) might become a future option of individualized therapy for patients with autoimmune diseases. In this study, we tested the possibility of generating monocyte-derived tolDC from patients with primary Sjögren's syndrome (pSS). We analyzed phenotype, cytokine production and ability to suppress Ro/La-specific immune responses.
  
  
  METHODS
  Monocyte-derived tolDC from patients with pSS were generated in the presence of dexamethasone, vitamin D3 and lipopolysaccharide (DexVD3 DC). The phenotype was analyzed by flow cytometry and the cytokine profile was investigated using a 25-plex Luminex assay and ELISA. The capacity to both stimulate Ro/La-specific T cells and suppress this response was evaluated by autologous mixed lymphocyte reaction (MLR).
  
  
  RESULTS
  DC generated from patients with pSS had a similar phenotype and cytokine profile to those from healthy controls. DexVD3 DC from pSS patients induced little antigen-specific T cell proliferation, but DexVD3 DC-primed lymphocytes successfully suppressed Ro/La-specific T cell responses.
  
  
  CONCLUSIONS
  DexVD3 DC presenting Ro/La antigens might be a promising new therapeutic option for patients with pSS.
authors.forename: Roman
authors.initials: R
authors.lastname: Volchenkov
authors.forename: Johan G
authors.initials: JG
authors.lastname: Brun
authors.forename: Roland
authors.initials: R
authors.lastname: Jonsson
authors.forename: Silke
authors.initials: S
authors.lastname: Appel
mesh_terms: D000328:Adult; D000368:Aged; D001324:Autoantigens / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D002762:Cholecalciferol / Q000276:immunology / Q000494:pharmacology; D018920:Coculture Techniques; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D003907:Dexamethasone / Q000276:immunology / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007108:Immune Tolerance / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D012261:Ribonucleoproteins / Q000276:immunology / Q000378:metabolism; D012859:Sjogren's Syndrome / Q000097:blood / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D014815:Vitamins / Q000276:immunology / Q000494:pharmacology; D000097585:SS-B Antigen; D000099207:SS-A Antigen
chemical_list: D001324:Autoantigens; D002762:Cholecalciferol; D016207:Cytokines; D003907:Dexamethasone; D008070:Lipopolysaccharides; D012261:Ribonucleoproteins; D014815:Vitamins; D000097585:SS-B Antigen; D000099207:SS-A Antigen
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