title: Evaluation of copper resistant bacteria from vineyard soils and mining waste for copper biosorption.
abstract: Vineyard soils are frequently polluted with high concentrations of copper due application of copper sulfate in order to control fungal diseases. Bioremediation is an efficient process for the treatment of contaminated sites. Efficient copper sorption bacteria can be used for bioremoval of copper from contaminated sites. In this study, a total of 106 copper resistant bacteria were examined for resistance to copper toxicity and biosorption of copper. Eighty isolates (45 from vineyard Mollisol, 35 from Inceptisol) were obtained from EMBRAPA (Empresa Brasileira de Pesquisa Agropecuária) experimental station, Bento Gonçalves, RS, Brazil (29°09'53.92″S and 51°31'39.40″W) and 26 were obtained from copper mining waste from Caçapava do Sul, RS, Brazil (30°29'43.48″S and 53'32'37.87W). Based on resistance to copper toxicity and biosorption, 15 isolates were identified by 16S rRNA gene sequencing. Maximal copper resistance and biosorption at high copper concentration were observed with isolate N2 which removed 80 mg L(-1) in 24 h. Contrarily isolate N11 (Bacillus pumilus) displayed the highest specific copper biosorption (121.82 mg/L/OD unit in 24 h). GenBank MEGABLAST analysis revealed that isolate N2 is 99% similar to Staphylococcus pasteuri. Results indicate that several of our isolates have potential use for bioremediation treatment of vineyards soils and mining waste contaminated with high copper concentration.
authors.affiliation: Departamento de Ciência do Solo, Universidade Federal do Rio Grande do Sul , Porto Alegre, RS , Brasil ; Department of Biology, Auburn University at Montgomery , P.O. Box 244023, Montgomery, AL , USA.
authors.forename: R
authors.initials: R
authors.lastname: Andreazza
authors.forename: S
authors.initials: S
authors.lastname: Pieniz
authors.forename: B C
authors.initials: BC
authors.lastname: Okeke
authors.forename: F A O
authors.initials: FA
authors.lastname: Camargo
keywords: Copper contamination, vineyard soil; bioremediation; mining waste, copper biosorption
references.citation: J Hazard Mater. 2009 Apr 30;163(2-3):869-73
references.pmid: 18722054
references.citation: Bioresour Technol. 2006 Jul;97(10):1237-42
references.pmid: 16324838
references.citation: Microbiol Res. 2009;164(6):613-23
references.pmid: 17993268
references.citation: Colloids Surf B Biointerfaces. 2005 Dec 10;46(2):101-7
references.pmid: 16289732
references.citation: J Hazard Mater. 2005 May 20;121(1-3):109-17
references.pmid: 15885411
references.citation: Sci Total Environ. 2007 Oct 15;385(1-3):12-9
references.pmid: 17692896
references.citation: FEMS Microbiol Rev. 1994 Jun;14(2):121-37
references.pmid: 8049096
references.citation: Mol Biol Evol. 2007 Aug;24(8):1596-9
references.pmid: 17488738
references.citation: J Hazard Mater. 2009 Aug 15;167(1-3):1170-7
references.pmid: 19272704
references.citation: Bioresour Technol. 2009 Jan;100(2):542-8
references.pmid: 18657416
references.citation: Bioresour Technol. 2009 May;100(9):2482-92
references.pmid: 19162475
references.citation: Colloids Surf B Biointerfaces. 2007 Jan 15;54(1):46-52
references.pmid: 16945511
references.citation: J Hazard Mater. 2009 Mar 15;162(2-3):874-9
references.pmid: 18599209
references.citation: Bioresour Technol. 2006 May;97(8):1041-9
references.pmid: 16324840
references.citation: Arch Environ Contam Toxicol. 2000 Nov;39(4):413-9
references.pmid: 11031300
references.citation: J Hazard Mater. 2009 Feb 15;162(1):50-6
references.pmid: 18562095
references.citation: J Hazard Mater. 2009 Aug 15;167(1-3):449-54
references.pmid: 19188025
references.citation: Bioresour Technol. 2009 Jan;100(1):330-7
references.pmid: 18513961
references.citation: Bioresour Technol. 2008 Sep;99(14):6059-69
references.pmid: 18255287
references.citation: Sci Total Environ. 2010 Mar 1;408(7):1501-7
references.pmid: 20117823