title: Characteristics of Vδ1(+) and Vδ2(+) γδ T cell subsets in acute liver allograft rejection.
abstract: BACKGROUND
  To investigate the characteristics of γδ T cells as well as the Vδ1(+) and Vδ2(+) subsets in the peripheral blood in liver allograft recipients.
  
  
  METHODS
  Sixty-three liver transplant recipients were enrolled in this study: 26 cases with acute allograft rejection (Gr-AR), and 37 cases with stable allograft liver function (Gr-SF). The frequencies of γδ T cells, the Vδ1(+) and Vδ2(+) subsets, and interleukin (IL)-10-producing Vδ1(+) γδ T cells in the peripheral blood were analyzed by flow cytometry. The relationship between liver function parameters and the Vδ1(+)/Vδ2(+) ratio was analyzed.
  
  
  RESULTS
  The frequency of the Vδ1(+) subset and the Vδ1(+)/Vδ2(+) ratio in Gr-SF was significantly higher than that in Gr-AR; in contrast, the frequency of the Vδ2(+) subset in Gr-SF was markedly lower than that in Gr-AR. In addition, there was no significant difference in the frequency of γδ T cells between the Gr-AR and Gr-SF groups. Moreover, there was a significant negative correlation between the Vδ1(+)/Vδ2(+) ratio with the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in Gr-AR.
  
  
  CONCLUSIONS
  Vδ1(+) γδ T cells may have a potential role in maintaining stable graft liver function, and Vδ2(+) γδ T cells may be associated with liver allograft rejection. The Vδ1(+)/Vδ2(+) ratio could serve as a prognostic marker for acute rejection after liver transplantation.
authors.affiliation: Peking University 302 Hospital, Beijing, China; Research Center for Liver Transplantation, Beijing 302 Hospital, Beijing, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Yu
authors.forename: Zhenwen
authors.initials: Z
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Hongbo
authors.initials: H
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Yanling
authors.initials: Y
authors.lastname: Sun
authors.forename: Haibin
authors.initials: H
authors.lastname: Su
authors.forename: Lei
authors.initials: L
authors.lastname: Jin
authors.forename: Fusheng
authors.initials: F
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Shi
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D064591:Allografts; D005260:Female; D006084:Graft Rejection / Q000097:blood*; D006801:Humans; D016753:Interleukin-10 / Q000097:blood*; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000097:blood*; D013601:T-Lymphocytes / Q000378:metabolism* / Q000473:pathology
chemical_list: C508609:IL10 protein, human; D016692:Receptors, Antigen, T-Cell, gamma-delta; D016753:Interleukin-10
keywords: ALT; AST; Acute rejection; CTLA; Gr-AR; Gr-SF; IL; Liver transplantation; NK; NKT; T helper; TGF-β; Th; acute rejection group; alanine aminotransferase; aspartate aminotransferase; cytotoxic T-lymphocyte antigen; interleukin; natural killer; natural killer T; stable function group; transforming growth factor; γδ T cell