title: Chemical composition and antifungal activity of Hyptis Suaveolens (L.) Poit leaves essential oil against Aspergillus species.
abstract: This study aimed to identify the constituents of the essential oil from Hyptis suaveolens (L.) leaves using a Gas Chromatograph - Mass Spectrometer and assess its inhibitory effect on some potentially pathogenic Aspergilli (A. flavus, A. parasiticus, A. ochraceus, A. fumigatus and A. niger). Eucaliptol (47.64 %) was the most abundant component in the oil, followed for gama-ellemene (8.15 %), beta-pynene (6.55 %), (+)-3-carene (5.16 %), trans-beta-cariophyllene (4.69 %) and germacrene (4.86 %). The essential oil revealed an interesting anti-Aspergillus property characterized by a Minimum Inhibitory Concentration and Minimum Fungicidal Concentration of 40 and 80 µL/mL, respectively. The oil at 80 and 40 µL/mL strongly inhibited the mycelial growth of A. fumigatus and A. parasiticus along 14 days. In addition, at 10 and 20 µL/mL the oil was able to cause morphological changes in A. flavus as decreased conidiation, leakage of cytoplasm, loss of pigmentation and disrupted cell structure suggesting fungal wall degeneration. These findings showed the interesting anti-Aspergillus property of H. suaveolens leaves essential oil supporting its possible rational use as alternative source of new antifungal compounds to be applied in the aspergillosis treatment.
authors.affiliation: Laboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba , João Pessoa, PB , Brasil.
authors.forename: Ana Carolina Pessoa
authors.initials: AC
authors.lastname: Moreira
authors.forename: Edeltrudes
authors.initials: E
authors.lastname: de Oliveira Lima
authors.forename: Paulo Alves
authors.initials: PA
authors.lastname: Wanderley
authors.forename: Egberto Santos
authors.initials: ES
authors.lastname: Carmo
authors.forename: Evandro Leite
authors.initials: EL
authors.lastname: de Souza
keywords: Hyptis suaveolens L. (Poit); anti-Aspergillus effect; essential oil
references.citation: J Hosp Infect. 2005 Mar;59(3):188-96
references.pmid: 15694975
references.citation: Phytochemistry. 2001 Jul;57(5):733-6
references.pmid: 11397441
references.citation: J Chromatogr. 1963 Aug;11:463-71
references.pmid: 14062605
references.citation: Phytochemistry. 2005 Dec;66(24):2851-6
references.pmid: 16289146
references.citation: Microbiol Res. 2008;163(3):337-44
references.pmid: 16870411
references.citation: Microbiol Res. 2004;159(3):295-304
references.pmid: 15462529
references.citation: Lancet Infect Dis. 2002 Sep;2(9):550-63
references.pmid: 12206971
references.citation: Planta Med. 1974 Sep;26(2):196-9
references.pmid: 4412047
references.citation: Surg Neurol. 2005 Mar;63(3):254-60; discussion 260
references.pmid: 15734518
references.citation: Fitoterapia. 2007 Jul;78(5):333-6
references.pmid: 17512135
references.citation: Res Microbiol. 2004 Sep;155(7):579-86
references.pmid: 15313260
references.citation: J Ethnopharmacol. 2005 Apr 8;98(1-2):195-200
references.pmid: 15763383