title: Tandem LC columns for the simultaneous retention of polar and nonpolar molecules in comprehensive metabolomics analysis.
abstract: The tandem use of hydrophilic interaction LC columns with RP columns in series configuration has resulted in the retention of both polar and nonpolar components in complex biological samples (mouse serum) in a single analysis. This approach successfully coupled various columns with orthogonal separation characteristics, employed a single solvent gradient program compatible with the two columns and used ESI coupled to a TOF mass spectrometer for detection. Ion suppression, a common problem in ESI, was virtually eliminated for components eluting with apparent capacity factors >0.7. Retention time reproducibility with the tandem columns performed over three days with over 100 injections was comparable to that observed for single columns alone. This method was applied to the analysis of a pooled mouse serum sample and afforded highly reproducible data for up to 3000 mass spectral features. This approach was implemented with a conventional LC-MS system and should find broad applicability in the comprehensive analysis of complex mixtures containing a wide range of compound polarities.
authors.affiliation: Department of Chemistry and Chemical Biology, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Chalcraft
authors.forename: Brian E
authors.initials: BE
authors.lastname: McCarry
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D053719:Tandem Mass Spectrometry
keywords: Comprehensive metabolomics; Ion suppression; Mixed-mode chromatography; Orthogonal separation; Tandem columns