title: The identification of two Trypanosoma cruzi I genotypes from domestic and sylvatic transmission cycles in Colombia based on a single polymerase chain reaction amplification of the spliced-leader intergenic region.
abstract: A single polymerase chain reaction (PCR) reaction targeting the spliced-leader intergenic region of Trypanosoma cruzi I was standardised by amplifying a 231 bp fragment in domestic (TcIDOM) strains or clones and 450 and 550 bp fragments in sylvatic strains or clones. This reaction was validated using 44 blind coded samples and 184 non-coded T. cruzi I clones isolated from sylvatic triatomines and the correspondence between the amplified fragments and their domestic or sylvatic origin was determined. Six of the nine strains isolated from acute cases suspected of oral infection had the sylvatic T. cruzi I profile. These results confirmed that the sylvatic T. cruzi I genotype is linked to cases of oral Chagas disease in Colombia. We therefore propose the use of this novel PCR reaction in strains or clones previously characterised as T. cruzi I to distinguish TcIDOMfrom sylvatic genotypes in studies of transmission dynamics, including the verification of population selection within hosts or detection of the frequency of mixed infections by both T. cruzi I genotypes in Colombia.
authors.affiliation: Universidad del Tolima, Laboratorio de Investigaciones en Parasitología Tropical, Altos de Santa HelenaIbagué, Colombia.
authors.forename: Lina Marcela
authors.initials: LM
authors.lastname: Villa
authors.forename: Felipe
authors.initials: F
authors.lastname: Guhl
authors.forename: Daniel
authors.initials: D
authors.lastname: Zabala
authors.forename: Juan David
authors.initials: JD
authors.lastname: Ramírez
authors.forename: Daniel Alfonso
authors.initials: DA
authors.lastname: Urrea
authors.forename: Diana Carolina
authors.initials: DC
authors.lastname: Hernández
authors.forename: Zulma
authors.initials: Z
authors.lastname: Cucunubá
authors.forename: Marleny
authors.initials: M
authors.lastname: Montilla
authors.forename: Julio César
authors.initials: JC
authors.lastname: Carranza
authors.forename: Karina
authors.initials: K
authors.lastname: Rueda
authors.forename: Jorge Eduardo
authors.initials: JE
authors.lastname: Trujillo
authors.forename: Gustavo Adolfo
authors.initials: GA
authors.lastname: Vallejo
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000635:transmission; D003105:Colombia; D021901:DNA, Intergenic / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics; D004197:Disease Reservoirs / Q000469:parasitology; D005838:Genotype; D007303:Insect Vectors / Q000469:parasitology; D016133:Polymerase Chain Reaction; D020038:RNA, Spliced Leader / Q000235:genetics*; D014224:Triatoma / Q000469:parasitology; D014225:Triatominae / Q000469:parasitology; D014349:Trypanosoma cruzi / Q000235:genetics*
chemical_list: D021901:DNA, Intergenic; D016054:DNA, Protozoan; D020038:RNA, Spliced Leader
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