title: Mitochondrial function and apoptosis of peripheral mononuclear cells (PBMCs) in the HIV infected patients.
abstract: HIV infection results in the development of immunodeficiency mainly due to the apoptosis of infected and by stander CD4 cells. The aim of the study was to follow the mitochondrial dependent pathway of apoptosis, one of the suggested mechanisms of above process. The inner mitochondrial membrane potential (MMP), Adenosine-5'-triphosphate (ATP) generation, apoptosis and necrosis markers of peripheral mononuclear cells (PBMCs) were compared in HIV infected patients and HIV negative control group. The correlation of blood viral load, TNFα concentration, CD4 cells count and duration of ARV therapy was considered. Additionally, group of HIV infected ARV-naive patients was involved for the follow-up study and the effects of one year of ARV therapy on measured parameters were studied. PBMCs of HIV infected individuals (especially without ARV therapy) demonstrated lower MMP and ATP generation and higher percentage of apoptotic/necrotic PBMCs. Correlation between blood TNFα level and mitochondrial dysfunction was observed. The first months of ARV therapy resulted in most significant restoration of mitochondrial function and living PBMCs count. HIV infection and ARV therapy have significant impact on mitochondrial function and apoptosis of PBMCs. They are driven by abnormal mitochondrial function apoptosis of immune cells which seems to be the key element leading to immunosuppression, thus an early intervention in this process by therapy can be beneficial for symptomatology of HIV infected patients.
authors.affiliation: Chair of Gastroenterology, Hepatology and Infectious Diseases, Department of Infectious Diseases Jagiellonian University, Collegium Medicum, Sniadeckich 5, 31-531 Kraków, Poland. monika.bociagajasik@gmail.com.
authors.forename: Monika
authors.initials: M
authors.lastname: Bociąga-Jasik
authors.forename: Joanna
authors.initials: J
authors.lastname: Góralska
authors.forename: Anna
authors.initials: A
authors.lastname: Polus
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Śliwa
authors.forename: Anna
authors.initials: A
authors.lastname: Gruca
authors.forename: Urszula
authors.initials: U
authors.lastname: Raźny
authors.forename: Anna
authors.initials: A
authors.lastname: Zdzienicka
authors.forename: Aleksander
authors.initials: A
authors.lastname: Garlicki
authors.forename: Tomasz
authors.initials: T
authors.lastname: Mach
authors.forename: Aldona
authors.initials: A
authors.lastname: Dembińska-Kieć
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D000704:Analysis of Variance; D018501:Antirheumatic Agents / Q000627:therapeutic use; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology; D016022:Case-Control Studies; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology / Q000503:physiopathology* / Q000821:virology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000502:physiology*; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D019562:Viral Load; D055815:Young Adult
chemical_list: D018501:Antirheumatic Agents; D015415:Biomarkers; D014409:Tumor Necrosis Factor-alpha; D000255:Adenosine Triphosphate