title: Scopulariopsis, a poorly known opportunistic fungus: spectrum of species in clinical samples and in vitro responses to antifungal drugs.
abstract: Ninety-nine isolates of clinical origin, tentatively identified as Scopulariopsis or Microascus, were morphologically and molecularly characterized by a combined analysis of the D1/D2 domains of the 28S rRNA gene and a fragment of the elongation factor 1-α gene (EF1-α) sequences. The most prevalent species was Scopulariopsis brevicaulis (49.4%), followed by Scopulariopsis gracilis (14.4%), Scopulariopsis brumptii (7.2%), Microascus cinereus (5.2%), the Scopulariopsis candida species complex (3.1%), and Microascus cirrosus (2.1%). The most common anatomic sites of isolation were the respiratory tract (61.6%), superficial tissue (19.2%), and deep tissue or fluid samples (19.2%). The antifungal susceptibilities of the isolates to eight drugs were tested in vitro, with all the drugs generally showing poor activity.
authors.affiliation: Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Sandoval-Denis
authors.forename: Deanna A
authors.initials: DA
authors.lastname: Sutton
authors.forename: Annette W
authors.initials: AW
authors.lastname: Fothergill
authors.forename: Josep
authors.initials: J
authors.lastname: Cano-Lira
authors.forename: Josepa
authors.initials: J
authors.lastname: Gené
authors.forename: C A
authors.initials: CA
authors.lastname: Decock
authors.forename: G S
authors.initials: GS
authors.lastname: de Hoog
authors.forename: Josep
authors.initials: J
authors.lastname: Guarro
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D009181:Mycoses / Q000453:epidemiology / Q000382:microbiology*; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D060645:Scopulariopsis / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D020648:Peptide Elongation Factor 1; D012331:RNA, Fungal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S
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