title: Surface modification of PLLA nano-scaffolds with laminin multilayer by LbL assembly for enhancing neurite outgrowth.
abstract: In this study, PLLA nanofibers are fabricated by electrospinning and their surfaces are modified by laminin/chitosan (LN/CS) polyelectrolyte multilayer. Surface C/N ratio determined by XPS analysis quantitatively indicates of discrete coating layers on the nanofibers. The amount of LN deposited sustainably increases with LbL assembly processing, approximately 60 ng mm(-2) LN per cycle of LN/CS deposition. The LN-modified PLLA scaffolds significantly induce neurite outgrowth of DRG neurons and NSC compared to the pure PLLA nanofibrous scaffolds. Furthermore, higher amounts of LN adsorbed assist in promoting cell proliferation than PLLA as-spun nanofibers. Therefore, a facile and efficient method to modify nano-scaffolds for the construction of a biomimetic scaffold to promote highly efficient neurite outgrowth is presented.
authors.affiliation: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou, 510632, China.
authors.forename: Liumin
authors.initials: L
authors.lastname: He
authors.forename: Shuo
authors.initials: S
authors.lastname: Tang
authors.forename: Molamma P
authors.initials: MP
authors.lastname: Prabhakaran
authors.forename: Susan
authors.initials: S
authors.lastname: Liao
authors.forename: Lingling
authors.initials: L
authors.lastname: Tian
authors.forename: Yuanming
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Xue
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D055664:Electrochemical Techniques; D004622:Embryo, Mammalian; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects; D019344:Lactic Acid / Q000737:chemistry*; D007797:Laminin / Q000737:chemistry*; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects; D016501:Neurites / Q000187:drug effects; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D055672:Static Electricity; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D007797:Laminin; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D048271:Chitosan
keywords: biomimetic; chitosan; laminin; polyelectrolyte multilayer; tissue engineering