title: No association of IFNG+874T/A SNP and NOS2A-954G/C SNP variants with nitric oxide radical serum levels or susceptibility to tuberculosis in a Brazilian population subset.
abstract: Tuberculosis (TB) is one of the most common infectious diseases in the world. Mycobacterium tuberculosis infection leads to pulmonary active disease in approximately 5-10% of exposed individuals. Both bacteria- and host-related characteristics influence latent infection and disease. Host genetic predisposition to develop TB may involve multiple genes and their polymorphisms. It was reported previously that interferon gamma (IFN-γ) and nitric oxide synthase 2 (NOS2) are expressed on alveolar macrophages from TB patients and are responsible for bacilli control; thus, we aimed this study at genotyping single nucleotide polymorphisms IFNG+874T/A SNP and NOS2A-954G/C SNP to estimate their role on TB susceptibility and determine whether these polymorphisms influence serum nitrite and NOx(-) production. This case-control study enrolled 172 TB patients and 179 healthy controls. Neither polymorphism was associated with susceptibility to TB. NOS2A-954G/C SNP was not associated with serum levels of nitrite and NOx(-). These results indicate that variants of IFNG+874T/A SNP and NOS2A-954G/C SNP do not influence TB susceptibility or the secretion of nitric oxide radicals in the study population.
authors.affiliation: Immunology and Immunogenetics Laboratory, Evandro Chagas Clinical Research Institute, Oswaldo Cruz Foundation, Avenida Brasil 4365, Manguinhos, 21045-900 Rio de Janeiro, RJ, Brazil ; Department of Genetics and Southwest National Primate Research Center, Texas Biomedical Research Institute, 7620 NW Loop 410, 78227-5301 San Antonio, TX, USA.
authors.forename: Ana Cristina C S
authors.initials: AC
authors.lastname: Leandro
authors.forename: Márcia Andrade
authors.initials: MA
authors.lastname: Rocha
authors.forename: Andreia
authors.initials: A
authors.lastname: Lamoglia-Souza
authors.forename: John L
authors.initials: JL
authors.lastname: VandeBerg
authors.forename: Valeria Cavalcanti
authors.initials: VC
authors.lastname: Rolla
authors.forename: Maria da Gloria
authors.initials: Mda G
authors.lastname: Bonecini-Almeida
mesh_terms: D000328:Adult; D001938:Brazil; D016022:Case-Control Studies; D005260:Female; D005609:Free Radicals / Q000097:blood; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005828:Genetics, Population; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood* / Q000235:genetics; D052247:Nitric Oxide Synthase Type II / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D014376:Tuberculosis / Q000097:blood / Q000235:genetics* / Q000473:pathology
chemical_list: D005609:Free Radicals; C573415:IFNG protein, human; D009569:Nitric Oxide; D007371:Interferon-gamma; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II
references.citation: Genes Immun. 2004 Jan;5(1):46-57
references.pmid: 14735149
references.citation: Infect Immun. 1998 Nov;66(11):5314-21
references.pmid: 9784538
references.citation: J Infect Dis. 1999 Dec;180(6):1994-2002
references.pmid: 10558957
references.citation: Tuberculosis (Edinb). 2006 Jan;86(1):11-9
references.pmid: 15925543
references.citation: J Exp Med. 1993 Dec 1;178(6):2243-7
references.pmid: 8245795
references.citation: J Immunol. 2003 Nov 15;171(10):5442-6
references.pmid: 14607949
references.citation: Eur J Immunogenet. 2002 Jun;29(3):237-40
references.pmid: 12047360
references.citation: J Occup Health. 2008;50(6):445-54
references.pmid: 18931463
references.citation: Genes Immun. 2005 Jun;6(4):358-63
references.pmid: 15815688
references.citation: J Infect. 2006 May;52(5):374-7
references.pmid: 16233916
references.citation: J Korean Med Sci. 1997 Dec;12(6):481-7
references.pmid: 9443084
references.citation: Annu Rev Immunol. 2013;31:475-527
references.pmid: 23516984
references.citation: Infect Immun. 1995 Aug;63(8):3231-4
references.pmid: 7622255
references.citation: Tuberculosis (Edinb). 2007 Jul;87(4):288-94
references.pmid: 17475563
references.citation: BMC Gastroenterol. 2010 Jun 17;10:64
references.pmid: 20565800
references.citation: Lancet. 1999 Jan 2;353(9146):72
references.pmid: 10023979
references.citation: Lancet. 2003 May 31;361(9372):1871-2
references.pmid: 12788577
references.citation: J Immunol. 1998 May 1;160(9):4490-9
references.pmid: 9574555
references.citation: Hum Immunol. 2010 Jul;71(7):692-6
references.pmid: 20353805
references.citation: J Exp Med. 2005 Dec 19;202(12):1649-58
references.pmid: 16352737
references.citation: Hum Immunol. 2000 Sep;61(9):863-6
references.pmid: 11053629
references.citation: Infect Immun. 1994 Dec;62(12):5673-8
references.pmid: 7960152
references.citation: Nitric Oxide. 2001 Feb;5(1):62-71
references.pmid: 11178938
references.citation: Immunol Rev. 2000 Dec;178:129-37
references.pmid: 11213797
references.citation: Am J Respir Crit Care Med. 2006 Aug 1;174(3):339-43
references.pmid: 16690980
references.citation: DNA Cell Biol. 2011 Jun;30(6):379-87
references.pmid: 21332391
references.citation: Eur J Immunogenet. 2002 Oct;29(5):371-4
references.pmid: 12358843
references.citation: Eur J Immunogenet. 1999 Feb;26(1):1-3
references.pmid: 10068907
references.citation: J Exp Med. 1996 May 1;183(5):2293-302
references.pmid: 8642338
references.citation: J Infect Dis. 2001 Aug 1;184(3):330-6
references.pmid: 11443559
references.citation: Int J Tuberc Lung Dis. 2007 Jan;11(1):113-5
references.pmid: 17217140
references.citation: Infect Genet Evol. 2011 Jul;11(5):1015-22
references.pmid: 21463712
references.citation: Lancet. 1998 Jan 24;351(9098):265-6
references.pmid: 9457101
references.citation: J Immunol. 2013 Jan 1;190(1):270-7
references.pmid: 23233724
references.citation: Tuberculosis (Edinb). 2007 Mar;87(2):166-71
references.pmid: 17023216
references.citation: Proc Soc Exp Biol Med. 1995 May;209(1):46-53
references.pmid: 7536940
references.citation: Mol Biol Rep. 2008 Dec;35(4):563-6
references.pmid: 17682837
references.citation: Tuberculosis (Edinb). 2007 May;87(3):225-30
references.pmid: 17276141
references.citation: Genes Immun. 2002 Feb;3(1):1-4
references.pmid: 11857052
references.citation: Science. 1993 Mar 19;259(5102):1739-42
references.pmid: 8456300
references.citation: Am J Respir Crit Care Med. 2003 Apr 1;167(7):970-5
references.pmid: 12531774
references.citation: J Immunol. 2003 Nov 1;171(9):4689-99
references.pmid: 14568944