title: Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil.
abstract: Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3.
authors.affiliation: Centro Universitário Franciscano , Santa Maria, RS , Brasil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: de Almeida Vaucher
authors.forename: Diogenes
authors.initials: D
authors.lastname: Dezen
authors.forename: Amauri Braga
authors.initials: AB
authors.lastname: Simonetti
authors.forename: Fernando Rosado
authors.initials: FR
authors.lastname: Spilki
authors.forename: Paulo Michel
authors.initials: PM
authors.lastname: Roehe
keywords: Bovine parainfluenza virus type 3; bPIV-3; phylogenetic analysis
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