title: Regulation of T lymphocyte subpopulations in specific pathogen-free chickens following experimental fowl adenovirus-ⅧVIII infection.
abstract: Two-day-old specific pathogen-free (SPF) chickens were divided into two groups. Group I was inoculated orally with fowl adenovirus Ⅷ (FAV-Ⅷ). Group II served as a negative control. Chickens were investigated at various days post-inoculation (dpi) by flow cytometric analysis for changes in T lymphocyte subpopulations in immune system and blood. In the thymus, CD3(+)T lymphocytes were increased at 25 dpi, with significant increases in the FAV infected noted at 1, 12, 20dpi (p<0.05). This was accompanied by a corresponding increase of CD4(+) and CD8(+) T lymphocytes. In the spleen, CD3(+) and CD4(+) T lymphocytes were increased significantly at 30 dpi (p<0.01) whereas CD8(+) and TCR γ δ(+) T lymphocytes were decreased at 1 (p<0.05), 30 dpi (p<0.01). An increase of CD3(+), CD4(+) and CD8(+) T lymphocytes was noticed in peripheral blood, and accompanied by a decrease of TCR γ δ(+) T lymphocytes. These results demonstrated that infection with FAV-Ⅷ causes significant fluctuations in T lymphocyte subpopulations in thymus, blood and spleen. It can be concluded that an infection with FAV-Ⅷ has profound effects on the immune system, especially on cell mediated immune competency.
authors.affiliation: Department of Basic Veterinary, College of Veterinary Science, Inner Mongolia Agricultural University , Huhhot, Inner Mongolia , China, 010018.
authors.forename: Chun-Jie
authors.initials: CJ
authors.lastname: Wang
authors.forename: Siriguleng
authors.initials: S
authors.lastname: Yu
authors.lastname: Ao-Ri-Ge-le
authors.forename: De-Gang
authors.initials: DG
authors.lastname: Jia
authors.forename: Hong-Qiang
authors.initials: HQ
authors.lastname: Yao
authors.forename: Huai-Ping
authors.initials: HP
authors.lastname: Zhao
authors.forename: H S
authors.initials: HS
authors.lastname: Lillehoj
authors.lastname: Si-Mu-Ji-de
authors.forename: Andrew Cl
authors.initials: AC
authors.lastname: Postnikoff
authors.forename: Si-Ri-Gu-Leng
authors.initials: SR
authors.lastname: Xu
keywords: Cell mediated immune competency; Fowl adenovirus Ⅷ; Immune system; Peripheral blood; T lymphocyte subpopulations
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