title: Flow-mediated stem cell labeling with superparamagnetic iron oxide nanoparticle clusters.
abstract: This study presents a strategy to enhance the uptake of superparamagnetic iron oxide nanoparticle (SPIO) clusters by manipulating the cellular mechanical environment. Specifically, stem cells exposed to an orbital flow ingested almost a 2-fold greater amount of SPIO clusters than those cultured statically. Improvements in magnetic resonance (MR) contrast were subsequently achieved for labeled cells in collagen gels and a mouse model. Overall, this strategy will serve to improve the efficiency of cell tracking and therapies.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, 61801, United States.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Clay
authors.affiliation: Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, 61801, United States.
authors.forename: Kwanghyun
authors.initials: K
authors.lastname: Baek
authors.affiliation: Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, Illinois, 61801, United States.
authors.forename: Artem
authors.initials: A
authors.lastname: Shkumatov
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, 61801, United States.
authors.forename: Mei-Hsiu
authors.initials: MH
authors.lastname: Lai
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, 61801, United States.
authors.forename: Cartney E
authors.initials: CE
authors.lastname: Smith
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, 61801, United States.
authors.forename: Max
authors.initials: M
authors.lastname: Rich
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, 61801, United States.
authors.forename: Hyunjoon
authors.initials: H
authors.lastname: Kong
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology; D058948:Cell Tracking; D002478:Cells, Cultured; D003287:Contrast Media / Q000737:chemistry*; D004705:Endocytosis; D005290:Ferric Compounds / Q000737:chemistry*; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D040542:Mechanotransduction, Cellular; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000166:cytology*; D051379:Mice; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D010455:Peptides; C000499:ferric oxide; C025252:poly-DL-succinimide; D001224:Aspartic Acid
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