title: Cellulose production by Gluconacetobacter kakiaceti GM5 in two batch process using vinasse as culture media.
abstract: The present study was conducted to evaluate the cellulose production by Gluconacetobacter kakiaceti GM5 by means of two aerobic treatments: the static discontinuous fermentation process (treatment 1) and the discontinuous fermentation process in a rotary shaker (treatment 2). All these experiments were carried out using vinasse as experimental culture media (VM) and were compared with standard media containing glucose at 2% (standard medium (SM)). A sample of each treatment was extracted every 24 h over a period of 168 h. The maximum rates of cellulose produced in treatment 1 using SM added up to 3.63 ± 0.18 g l(-1), and to 4.15 ± 0.16 g l(-1) when VM was used. The amount of cellulose produced in treatment 2 using SM was 2.95 ± 0.09 g l(-1) (which suggests an increase of 37%), and added up to 1.84 ± 0.07 g l(-1) when using VM. A better global yield of both treatments in terms of sugar consumption after 168 h was obtained when using VM: 32% in treatment 1, whereas in treatment 2 it was 9%. A 20% decrease on vinasse COD (Chemical Oxygen Demand) values was found to be yet another important advantage of working with this strain.
authors.affiliation: Departamento de Nutrición y Bioquímica, Facultad de Ciencias, Pontificia Universidad Javeriana, Carrera 7ª N° 43-82, Bogotá, Colombia E-mail: moritzvr@yahoo.com; Escuela de Microbiología, Universidad de Antioquia, Calle 67 No. 53-108, Bloque 5, Medellín, Colombia.
authors.forename: Möritz
authors.initials: M
authors.lastname: Velásquez-Riaño
authors.forename: Nelson
authors.initials: N
authors.lastname: Lombana-Sánchez
authors.forename: Andrés Felipe
authors.initials: AF
authors.lastname: Villa-Restrepo
authors.forename: Erika Pamela
authors.initials: EP
authors.lastname: Fernández-Calle
mesh_terms: D000332:Aerobiosis; D002482:Cellulose / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry*; D041821:Gluconacetobacter / Q000378:metabolism*
chemical_list: D003470:Culture Media; D002482:Cellulose