title: Design and testing of paramagnetic liposome-based CEST agents for MRI visualization of payload release on pH-induced and ultrasound stimulation.
abstract: The development of nanomedicines in cancer therapy is constantly growing because of the advantages associated with the use of nanosized drug delivery systems. Among them, the possibility of accurate spatiotemporal control of the release of the chemotherapeutic from the carrier is one of the most interesting and clinically relevant. To further improve the therapy outcome, the clinical translation of imaging protocols for the in vivo visualization of the release step is of paramount importance. In this work, the combination of the great chemical versatility of liposomes and the outstanding potential of MRI chemical exchange saturation transfer agents has been successfully harnessed to image the selective release of the liposomal content stimulated by endogenous (variation of pH) and externally applied (nonfocused ultrasound) stimuli. The use of clinically safe components (both liposomes and MRI agents) and the good results obtained in vitro hold promise for a successful future in vivo translation.
authors.affiliation: Molecular Imaging Center, Department of Molecular Biotechnology and Health Sciences, University of Turin, Via Nizza 52, 10126, Turin, Italy.
authors.forename: Daniela Delli
authors.initials: DD
authors.lastname: Castelli
authors.forename: Cinzia
authors.initials: C
authors.lastname: Boffa
authors.forename: Pierangela
authors.initials: P
authors.lastname: Giustetto
authors.forename: Enzo
authors.initials: E
authors.lastname: Terreno
authors.forename: Silvio
authors.initials: S
authors.lastname: Aime
mesh_terms: D015195:Drug Design; D006843:Hydrocarbons, Chlorinated / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D028581:Lanthanoid Series Elements / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D008279:Magnetic Resonance Imaging; D059346:Magnets / Q000737:chemistry*; D014465:Ultrasonics
chemical_list: D006843:Hydrocarbons, Chlorinated; D028581:Lanthanoid Series Elements; D008081:Liposomes
references.citation: Magn Reson Imaging. 1999 Jan;17(1):83-9
references.pmid: 9888401
references.citation: J Inorg Biochem. 2008 May-Jun;102(5-6):1112-9
references.pmid: 18329102
references.citation: Cancer Treat Rev. 2013 Jun;39(4):305-12
references.pmid: 23207060
references.citation: J Control Release. 2011 Sep 5;154(2):196-202
references.pmid: 21621569
references.citation: Contrast Media Mol Imaging. 2006 Sep-Oct;1(5):184-8
references.pmid: 17193695
references.citation: Mitochondrion. 2012 Mar;12(2):190-201
references.pmid: 22138492
references.citation: Inorg Chem. 2013 Jun 17;52(12):7130-8
references.pmid: 23738541
references.citation: Angew Chem Int Ed Engl. 2005 Aug 26;44(34):5513-5
references.pmid: 16052647
references.citation: Contrast Media Mol Imaging. 2008 Jul-Aug;3(4):136-49
references.pmid: 18683280
references.citation: Magn Reson Med. 2012 Apr;67(4):1106-13
references.pmid: 22392814
references.citation: Curr Top Med Chem. 2012;12(15):1693-712
references.pmid: 22978336
references.citation: Expert Opin Drug Deliv. 2012 Apr;9(4):367-81
references.pmid: 22400808
references.citation: Contrast Media Mol Imaging. 2008 Jan-Feb;3(1):38-43
references.pmid: 18335476
references.citation: Curr Med Chem. 2012;19(19):3230-40
references.pmid: 22612697
references.citation: Bioorg Med Chem. 2011 Feb 1;19(3):1097-105
references.pmid: 20691598
references.citation: J Control Release. 2012 Jul 20;161(2):317-27
references.pmid: 22565055
references.citation: Chem Rev. 2010 May 12;110(5):3019-42
references.pmid: 20415475
references.citation: Angew Chem Int Ed Engl. 2005 Mar 11;44(12):1813-5
references.pmid: 15723362
references.citation: Acta Radiol. 2010 Oct;51(8):910-23
references.pmid: 20828299
references.citation: Magn Reson Med. 2013 Jan;69(1):179-87
references.pmid: 22378016
references.citation: J Control Release. 2008 Mar 20;126(3):187-204
references.pmid: 18261822
references.citation: Mol Imaging. 2012 Feb;11(1):47-57
references.pmid: 22418027
references.citation: J Am Chem Soc. 2009 Feb 4;131(4):1380-1
references.pmid: 19173663
references.citation: Contrast Media Mol Imaging. 2010 Mar-Apr;5(2):78-98
references.pmid: 20419761
references.citation: Magn Reson Med. 2011 Apr;65(4):927-48
references.pmid: 21337419
references.citation: J Control Release. 2012 Jul 20;161(2):328-37
references.pmid: 22626940
references.citation: Magn Reson Med. 2005 Apr;53(4):790-9
references.pmid: 15799055
references.citation: J Magn Reson. 2000 Mar;143(1):79-87
references.pmid: 10698648