title: Basic fibroblast growth factor expression is implicated in mesenchymal stem cells response to light-induced retinal injury.
abstract: Neurotrophic factors are involved in neuroprotection and its expression in mesenchymal stem cells (MSCs) may change during light-induced retinal injury. In this study, neurotrophic factor expression in MSCs was investigated after stimulation by supernatants of homogenized retina (SHR) from normal and light-injured rats. Conditioned media from control MSCs (CM-MSCs), MSCs stimulated by normal SHR (CM-NSHR), and MSCs stimulated by light-injured SHR (CM-ISHR) were examined regarding their ability to prevent degeneration of retinal explants. Basic fibroblast growth factor (bFGF) in MSCs was knockdown by lentivirus-mediated mRNA interference. Transfected MSCs were stimulated by SHR, and retinal preservation was reevaluated in the resultant conditioned media. We detected significant up-regulation of bFGF in CM-ISHR, accompanied by superior retinal neurotrophic effects in CM-ISHR over CM-NSHR and CM-MSCs. Down-regulation of bFGF in MSCs effectively inhibited this protective effect. Adding neutralizing antibody against bFGF to CM-ISHR also induced a similar effect. It is thus concluded that retinal injury may enhance neurotrophic factor expression in MSCs and promote the repair process. bFGF may play a critical role in MSCs' response to retinal injury.
authors.affiliation: Department of Ophthalmology, First Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Fuzhou, 350005, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Wang
authors.forename: Guoxing
authors.initials: G
authors.lastname: Xu
authors.forename: Jian
authors.initials: J
authors.lastname: Guo
mesh_terms: D000818:Animals; D002452:Cell Count; D020934:Ciliary Neurotrophic Factor / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D005136:Eye Proteins / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D016086:Lentivirus / Q000378:metabolism; D008027:Light; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects* / Q000821:virology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000293:injuries* / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D014162:Transfection; D015854:Up-Regulation / Q000187:drug effects / Q000528:radiation effects; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D020934:Ciliary Neurotrophic Factor; D017077:Culture Media, Conditioned; D005136:Eye Proteins; D051028:bcl-2-Associated X Protein; D016222:Fibroblast Growth Factor 2; D007770:L-Lactate Dehydrogenase
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