title: Tissue microarray analysis reveals the expression and prognostic significance of phosphorylated AktThr³⁰⁸ in oral squamous cell carcinoma.
abstract: OBJECTIVE
  We aimed to investigate the association between the expression of phosphorylated Akt(Thr308) (p-Akt(Thr308)) in oral squamous cell carcinoma (OSCC) tissues and clinicopathological parameters of OSCC patients and to verify the validity of p-Akt(Thr308) as a prognostic biomarker.
  
  
  METHODS
  One hundred and ninety-one patients with OSCC were recruited for the study. We tested the expression of p-Akt(Thr308) by immunohistochemistry (IHC) with tissue microarray (TMA) and analyzed with digital pathology analysis software. The clinicopathological parameters of all patients were collected from follow-up.
  
  
  RESULTS
  P-Akt(Thr308) was detected in 95.2% of OSCC patients. The expression of p-Akt(Thr308) was significantly correlated with local recurrence and five-year survival rate. High expression of p-Akt(Thr308) in OSCC was associated with poor prognosis.
  
  
  CONCLUSIONS
  P-Akt(Thr308) might be a candidate biomarker for the prediction of OSCC prognosis.
authors.affiliation: DDS Candidate, State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University; the Stomatology Hospital of Tianjin Medical University, Tianjin, China.
authors.forename: Yanzhong
authors.initials: Y
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Jiayi
authors.initials: J
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Zeng
authors.forename: Ga
authors.initials: G
authors.lastname: Liao
authors.forename: Hongxia
authors.initials: H
authors.lastname: Dan
authors.forename: Qianming
authors.initials: Q
authors.lastname: Chen
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D010766:Phosphorylation; D011379:Prognosis; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015996:Survival Rate; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; D051057:Proto-Oncogene Proteins c-akt