title: Bioassay assessment of metarhizium anisopliae (metchnikoff) sorokin (deuteromycota: hyphomycetes) against Oncometopia facialis (signoret) (hemiptera: cicadellidae).
abstract: Citrus Variegated Chlorosis (CVC) is an economically important, destructive disease in Brazil and is caused by Xylella fastidiosa and transmitted by sharpshooter insects. In this study, the efficacy of the fungus Metarhizium anisopliae in controlling the sharpshooter Oncometopia facialis was studied by bioassay conditions. In the bioassay, insects were sprayed with a suspension containing 5 X 10(7) conidia mL(-1). Adults captured in the field were treated in groups of 10 in a total of 11 replications per treatment. Significant differences between the natural mortality and the mortality of insects treated with the fungus were observed 6 days after inoculations (P<0.05). These significant differences increased until 10 days after treatment. The fungus caused 87.1% mortality, with the LT50 varying from 5 to 6 days. The LC50 was 1.2 X 10(6) conidia mL(-1), varying from 7.7 X 10(5) to 2 X 10(6) conidia mL(-1). The results showed that the sharpshooter O. facialis was susceptible to the entomopathogenic action of M. anisopliae in controlled condition during bioassay.
authors.affiliation: Departamento de Bioquímica, Universidade Estadual Paulista "Júlio de Mesquita Filho" , Araraquara, SP , Brasil.
authors.forename: Wolney Dalla
authors.initials: WD
authors.lastname: Pria Júnior
authors.forename: Paulo Teixeira
authors.initials: PT
authors.lastname: Lacava
authors.forename: Claudio Luiz
authors.initials: CL
authors.lastname: Messias
authors.forename: João Lúcio
authors.initials: JL
authors.lastname: Azevedo
authors.forename: Pedro Magalhães
authors.initials: PM
authors.lastname: Lacava
keywords: Citrus Variegated Chlorosis (CVC); bioassay; entomopathogen; microbial control
references.citation: Appl Environ Microbiol. 2001 Sep;67(9):4091-5
references.pmid: 11526010
references.citation: Nature. 2000 Jul 13;406(6792):151-9
references.pmid: 10910347
references.citation: Curr Microbiol. 2005 Apr;50(4):223-8
references.pmid: 15902471
references.citation: Curr Microbiol. 1993 Sep;27(3):137-42
references.pmid: 23835746
references.citation: J Econ Entomol. 2003 Apr;96(2):264-71
references.pmid: 14994789
references.citation: Plant Dis. 2002 Nov;86(11):1237-1239
references.pmid: 30818474
references.citation: Plant Dis. 2002 Oct;86(10):1056-1066
references.pmid: 30818496
references.citation: Can J Microbiol. 2004 Jan;50(1):41-9
references.pmid: 15052320