title: Effect of danhong injection on expressions of macrophage scavenger receptor 1 and sub-family A member 1 in human U937 cells.
abstract: OBJECTIVE
  To study the effects of Danhong injection (DHI) on expression of the macrophage scavenger receptor 1 (MSR1) and ATP-binding cassette, sub-family A member 1 (ABCA1) genes, which encode scavenger receptor-A I (SR-AI) and ATP-binding cassette transporter 1 (ABCA1), respectively, as a potential anti-atherosclerotic mechanism.
  
  
  METHODS
  Human U937 cells were stimulated by incubation with 100 nM phorbol 12-myristate 13-acetate (PMA) for 48 h. These stimulated, monocyte-like cells were then incubated for 24 h with 50 mg/L oxidized low-density lipoprotein (ox-LDL, to induce foam cell formation), together with a liver X receptor (LXR) agonist or with different DHI concentrations. MSR1 and ABCA1 mRNA levels were measured by fluorescence-based quantitative PCR.
  
  
  RESULTS
  Compared with control cells (which received only ox-LDL), cells treated with both ox-LDL and 10 micromol/L LXR agonist showed lower MSR1 expression (but this effect was not statistically significant, P > 0.05) and higher ABCA1 expression (P < 0.01). Cells that received ox-LDL and 3 mL/L DHI possessed higher MSR1 mRNA levels than the controls, whereas cells treated with ox-LDL and higher DHI concentrations (10, 30 or 60 mL/L) showed lower MSR1 expression levels (but the differences observed between DHI concentration groups were not statistically significant, P > 0.05). ABCA1 expression in cells treated with ox-LDL and 3, 10 or 30 mL/L DHI was higher than in the control cells, and increased with increasing DHI concentration (P < 0.05). ABCA1 expression in cells treated with ox-LDL and the highest DHI concentration tested (60 mL/L) was not significantly different from that in the controls. ABCA1 mRNA levels in cells treated with ox-LDL and DHI were similar to, or lower than, those in cells treated with ox-LDL and the LXR agonist.
  
  
  CONCLUSIONS
  DHI does not affect MSR1 mRNA levels in ox-LDL-treated U937 cells. However, at certain concentrations (10 and 30 mL/L), DHI significantly increases ABCA1 mRNA levels. Therefore, the anti-atherosclerotic action of DHI might be mediated by an increased expression of ABCA1.
authors.affiliation: Department of Geriatric Cardiology, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, China. suxianming2011@163.com
authors.forename: Xianming
authors.initials: X
authors.lastname: Su
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Zhi
mesh_terms: D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D051117:Scavenger Receptors, Class A / Q000235:genetics* / Q000378:metabolism; D020298:U937 Cells
chemical_list: C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; D004365:Drugs, Chinese Herbal; C495139:MSR1 protein, human; D051117:Scavenger Receptors, Class A