title: Isolation and characterization of luteal cells in buffalo (Bubalus bubalis).
abstract: The objective of the study was to evaluate the morphological and functional characteristics of luteal cells isolated from buffalo ovary. Luteal cells exhibited columnar morphology and contact inhibition at the stage of confluence. Protein concentrations increased linearly from Day 3 to Day 7 of culture. DNA concentrations increased from Day 3 to Day 5 and then declined to Day 7 of culture. PGF2alpha concentrations decreased progressively from Day 3 to Day 7 of culture. It was concluded that buffalo luteal cells could serve as an excellent model for studying the specific role of PGF2alpha in maternal recognition of pregnancy and implantation.
authors.affiliation: National Institute of Animal Nutrition and Physiology Adugodi, Bangalore--560 030. sukanta781@gmail.com
authors.forename: S
authors.initials: S
authors.lastname: Mondal
authors.forename: S
authors.initials: S
authors.lastname: Nandi
authors.forename: I J
authors.initials: IJ
authors.lastname: Reddy
mesh_terms: D000818:Animals; D002020:Buffaloes; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D051546:Cyclooxygenase 2 / Q000235:genetics; D015237:Dinoprost / Q000502:physiology; D005260:Female; D008184:Luteal Cells / Q000502:physiology*; D011247:Pregnancy
chemical_list: D015237:Dinoprost; D051546:Cyclooxygenase 2