title: Purification and partial characterization of bacillomycin L produced by Bacillus amyloliquefaciens K103 from lemon.
abstract: Bacillus amyloliquefaciens K103 isolated from a lemon sample was used as a biocontrol agent to suppress Rhizoctonia solani Kühn and other fungal plant pathogens. Two antifungal compounds were purified from the culture broth using acid precipitation, gel permeation chromatography, and reversed-phase high-performance liquid chromatography. Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis indicated that the antifungal compounds were two isomers similar to bacillomycin L. One of the predominant active fractions was subjected to quadrupole time-of-flight mass spectrometry and amino acid analysis to determine its structural characteristics, revealing that the antifungal compound with a molecular mass of 1,034.5464 was identical to bacillomycin L. This is the second report of lemon microflora producing bacillomycin L or any antifungal compound, suppressing the growth of R. solani Kühn. Meanwhile, the study provided insights into the enormous potential of food microbial resources and bacillomycin L antibiotics in biological control and sustainable agriculture.
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.
authors.forename: Bao
authors.initials: B
authors.lastname: Zhang
authors.forename: Chunjuan
authors.initials: C
authors.lastname: Dong
authors.forename: Qingmao
authors.initials: Q
authors.lastname: Shang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cong
authors.forename: Weijia
authors.initials: W
authors.lastname: Kong
authors.forename: Pinglan
authors.initials: P
authors.lastname: Li
mesh_terms: D000596:Amino Acids; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification*; D001407:Bacillus / Q000737:chemistry* / Q000378:metabolism; D002957:Citrus / Q000737:chemistry* / Q000382:microbiology; D008970:Molecular Weight; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification; D012232:Rhizoctonia / Q000187:drug effects
chemical_list: D000596:Amino Acids; D000935:Antifungal Agents; D010456:Peptides, Cyclic; C015015:bacillomycin L