title: Sequence variability in three mitochondrial genes among four roundworm species from wild animals in China.
abstract: Sequence variability in three mitochondrial DNA (mtDNA) regions, namely portions of cytochrome c oxidase subunit 1 (pcox1), NADH dehydrogenase subunit 1 (pnad1) and NADH dehydrogenase subunit 4 (pnad4), for Toxocara canis. Baylisacaris transfuga. Ascaris suum and Parascaris equorum from Canis lupus. Ursus thibetanus. Sus scrofa and Equus burchelli in China were examined. The lengths of the sequences of pcox1, pnad1 and pnad4 were 711 bp, 648 bp and 666 bp, respectively. No intra-species differences were detected in pcox1 for the four examined ascarid species, in pnad1 for T. canis. A. suum and P. equorum, and in pnad4 for B. transfuga and P. equorum. Sequence differences in pnad4 for six roundworm samples of T. canis and P. equorum were 0-0.1% and 0-0.3%, respectively, and were 0-0.3% in pnad1 for six roundworm samples isolate of B. transfuga. The inter-specific sequence differences among four species were 8.7-12.4% for pcox1, 13.9-17.7% for pnad1, and 14.0-25.7% for pnad4. Phylogenetic analyses suggested that the three mtDNA fragments could be used to identify ascarid species in families Ascaridiae and Toxocaridae.
authors.affiliation: College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University , Xinfeng Road, Daqing, Heilongjiang Province , P. R. China and.
authors.forename: Qiao-Cheng
authors.initials: QC
authors.lastname: Chang
authors.forename: Jun-Feng
authors.initials: JF
authors.lastname: Gao
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Sheng
authors.forename: Yan
authors.initials: Y
authors.lastname: Lou
authors.forename: Xu
authors.initials: X
authors.lastname: Zheng
authors.forename: Chun-Ren
authors.initials: CR
authors.lastname: Wang
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000469:parasitology*; D002681:China; D050259:Genes, Mitochondrial; D014644:Genetic Variation; D009348:Nematoda / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
keywords: Ascarid; mitochondrial DNA; phylogenetic analyses; sequence variability; wild animals