title: Behavior of enteroaggregative Escherichia coli, non-O157-shiga toxin-producing E. coli, enteroinvasive E. coli, enteropathogenic E. coli and enterotoxigenic E. coli strains on mung bean seeds and sprout.
abstract: The behavior of enteroaggregative Escherichia coli (EAEC), non-O157 shiga toxin-producing E. coli (non-O157-STEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enteropathogenic E. coli (EPEC) on mung bean seeds at 25±2 °C and during germination and sprouting of mung bean seeds at 20±2 ° and 30±2 °C and on mung bean sprouts at 3±2 °C was determined. When mung bean seeds were inoculated with EAEC, non-O157 STEC, EIEC, EPEC or ETEC strains, all these diarrheagenic E. coli pathotypes (DEPs) survived at least 90 days on mung bean seeds at 25±2 °C. All DEPs grew during germination and sprouting of seeds, reaching counts of approximately 5 Log and 7 Log CFU/g after 2 days at 20±2 ° and 30±2 °C, respectively. However, when the sprouts were inoculated after 1 day of seeds germination and stored at 20±2 ° or 30±2 °C, no growth was observed for any DEPs during sprouting at 20±2 °C per 9 d; however, a significant increase in the concentration of DEPs of approximately 0.7 log CFU/g was observed during sprouting at 30±2 °C after 1 day of sprout contamination. Refrigeration reduced the number of viable DEPs strains on sprouts after 10 days in storage; nevertheless, these decreases have no practical significance in the safety of the sprouts.
authors.affiliation: Centro de Investigaciones Químicas. Instituto de Ciencias Básicas e Ingeniería, Universidad Autónoma del Estado de Hidalgo, Centro Universitario, Carretera Pachuca-Tulancingo Km. 4.5. Mineral de la Reforma, Hidalgo, CP 42183, Mexico.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Gómez-Aldapa
authors.forename: Esmeralda
authors.initials: E
authors.lastname: Rangel-Vargas
authors.forename: Haydee
authors.initials: H
authors.lastname: Bautista-De León
authors.forename: Ma Estela
authors.initials: ME
authors.lastname: Vázquez-Barrios
authors.forename: Alberto J
authors.initials: AJ
authors.lastname: Gordillo-Martínez
authors.forename: Javier
authors.initials: J
authors.lastname: Castro-Rosas
mesh_terms: D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000502:physiology*; D007887:Fabaceae / Q000382:microbiology*; D005516:Food Microbiology; D050296:Microbial Viability; D018520:Plant Shoots / Q000382:microbiology*; D012639:Seeds / Q000382:microbiology*; D013696:Temperature; D013995:Time
keywords: EAEC; EIEC; EPEC; ETEC; Mung bean sprout; STEC