title: Chromatographic methods for simultaneous determination of diiodohydroxyquinoline and metronidazole in their binary mixture.
abstract: Two chromatographic methods were developed for analysis ofdiiodohydroxyquinoline (DIHQ) and metronidazole (MTN). In the first method, diiodohydroxyquinoline and metronidazole were separated on TLC silica gel 60F254 plate using chloroform: acetone: glacial acetic acid (7.5: 2.5: 0.1, by volume) as mobile phase. The obtained bands were then scanned at 254 nm. The second method is a RP-HPLC method in which diiodohydroxyquinoline and metronidazole were separated on a reversed-phase C18 column using water : methanol (60 :40, V/V, PH=3.6 )as mobile phase at a flow rate of 0.7 mL.min-1 and UV detection at 220 nm. The mentioned methods were successfully used for determination of diiodohydroxyquinoline and metronidazole in pure form and in their pharmaceutical formulation.
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmed Hegazy St., Beni-Suef Egypt.
authors.forename: Nouruddin Wageih
authors.initials: NW
authors.lastname: Ali
authors.forename: Mohammed
authors.initials: M
authors.lastname: Gamal
authors.forename: Mohammed
authors.initials: M
authors.lastname: Abdelkawy
mesh_terms: D002021:Buffers; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D002845:Chromatography / Q000592:standards; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D002855:Chromatography, Thin Layer; D006863:Hydrogen-Ion Concentration; D004103:Iodoquinol / Q000032:analysis*; D008795:Metronidazole / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods* / Q000592:standards
chemical_list: D002021:Buffers; D012997:Solvents; D008795:Metronidazole; D004103:Iodoquinol