title: First report of isolation of Mycobacterium novocastrense from water supplies.
abstract: We herein present the first documented report associated with the isolation of Mycobacterium novocastrense from environment. The identification and characterization of four unrelated isolates, one from the surface water and the other three from hospital water, were achieved by various conventional and molecular tests including a genus-specific PCR for Mycobacterium based on 65-kDa heat shock protein (hsp) gene and 16S rDNA sequencing. Our findings might shed further light on the natural habitat of this rare Mycobacterium.
authors.affiliation: Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan.
authors.forename: Ramin
authors.initials: R
authors.lastname: Dibaj
authors.forename: Davood
authors.initials: D
authors.lastname: Azadi
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Karami
authors.forename: Abass Daei
authors.initials: AD
authors.lastname: Naser
authors.forename: Hasan
authors.initials: H
authors.lastname: Shojaei
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D009170:Nontuberculous Mycobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology; D014881:Water Supply / Q000032:analysis*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: 16S rDNA; Mycobacterium; hsp65