title: The lutheran/basal cell adhesion molecule promotes tumor cell migration by modulating integrin-mediated cell attachment to laminin-511 protein.
abstract: Cell-matrix interactions are critical for tumor cell migration. Lutheran (Lu), also known as basal cell adhesion molecule (B-CAM), competes with integrins for binding to laminin α5, a subunit of LM-511, a major component of basement membranes. Here we show that the preferential binding of Lu/B-CAM to laminin α5 promotes tumor cell migration. The attachment of Lu/B-CAM transfectants to LM-511 was slightly weaker than that of control cells, and this was because Lu/B-CAM disturbed integrin binding to laminin α5. Lu/B-CAM induced a spindle cell shape with pseudopods and promoted cell migration on LM-511. In addition, blocking with an anti-Lu/B-CAM antibody led to a flat cell shape and inhibited migration on LM-511, similar to the effects of an activating integrin β1 antibody. We conclude that tumor cell migration on LM-511 requires that Lu/B-CAM competitively modulates cell attachment through integrins. We suggest that this competitive interaction is involved in a balance between static and migratory cell behaviors.
authors.affiliation: From the Laboratory of Clinical Biochemistry, Tokyo University of Pharmacy and Life Sciences, Hachioji, 192-0392, Japan.
authors.forename: Yamato
authors.initials: Y
authors.lastname: Kikkawa
authors.forename: Takaho
authors.initials: T
authors.lastname: Ogawa
authors.forename: Ryo
authors.initials: R
authors.lastname: Sudo
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yamada
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Katagiri
authors.forename: Kentaro
authors.initials: K
authors.lastname: Hozumi
authors.forename: Motoyoshi
authors.initials: M
authors.lastname: Nomizu
authors.forename: Jeffrey H
authors.initials: JH
authors.lastname: Miner
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000378:metabolism / Q000473:pathology; D002448:Cell Adhesion / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D048430:Cell Shape; D057809:HEK293 Cells; D006801:Humans; D016023:Integrins / Q000235:genetics / Q000378:metabolism*; D007797:Laminin / Q000235:genetics / Q000378:metabolism*; D008188:Lutheran Blood-Group System / Q000235:genetics / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: C090228:BCAM protein, human; C477645:Bcam protein, mouse; D015815:Cell Adhesion Molecules; D016023:Integrins; D007797:Laminin; D008188:Lutheran Blood-Group System; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; C097043:laminin alpha5
keywords: Cell Adhesion; Cell Migration; Extracellular Matrix; Integrin; Invasion; Laminin
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