title: Anti-inflammatory effect of qingpeng ointment in atopic dermatitis-like murine model.
abstract: Qingpeng ointment (QP) is a Chinese medicine which has been used in treatment of atopic dermatitis (AD) in China. AD-like lesions were induced in BALB/c mice by repeated application of 2,4-dinitrofluorobenzene (DNFB) on shaved backs. The mice were then treated for 2 weeks with QP of different concentrations and Mometasone Furoate cream (MF), respectively. Macroscopic and microscopic changes of the skin lesions were observed after the treatment. The levels of serum immunoglobulin (Ig) E, tissue interferon (IFN)- γ , and interleukin (IL)-4 and IL-17A and the levels of involucrin, filaggrin, and kallikrein7 in epidermis were measured. The results show severe dermatitis with immune profiles similar to human acute AD. A significant infiltration of CD4(+) T and mast cells was observed in dermis of lesion but inhibited by QP after a 2-week treatment with it. The production of IgE, IL-4 and the mRNA expression of IL-17A were also suppressed, but the level of IFN- γ was increased. MF suppressed all production of these cytokines and IgE. Accordingly, the mechanism of QP on AD might correlate with its ability of modulating the immune dysfunctions rather than suppressing them. It had no effect on expressions of involucrin and filaggrin, except that its vehicle decreased the level of kallikrein7.
authors.affiliation: Department of Dermatology, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, China.
authors.forename: Yun-Zhu
authors.initials: YZ
authors.lastname: Li
authors.forename: Xue-Yan
authors.initials: XY
authors.lastname: Lu
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.forename: Lin-Feng
authors.initials: LF
authors.lastname: Li
references.citation: Allergy. 2003 Feb;58(2):139-45
references.pmid: 12622745
references.citation: Clin Immunol. 2008 Jun;127(3):385-93
references.pmid: 18373953
references.citation: J Clin Invest. 2004 Mar;113(5):651-7
references.pmid: 14991059
references.citation: J Allergy Clin Immunol. 2006 Jan;117(1):176-83
references.pmid: 16387603
references.citation: J Pharmacol Sci. 2012;118(3):391-400
references.pmid: 22382656
references.citation: Ann Hematol. 2008 Nov;87(11):899-904
references.pmid: 18600325
references.citation: Eur J Immunol. 2009 Jul;39(7):1716-25
references.pmid: 19544308
references.citation: Int Immunol. 1997 Mar;9(3):461-6
references.pmid: 9088984
references.citation: Curr Opin Allergy Clin Immunol. 2007 Oct;7(5):382-6
references.pmid: 17873576
references.citation: Curr Opin Immunol. 2011 Dec;23(6):778-83
references.pmid: 22018455
references.citation: Br J Dermatol. 2001 Jun;144(6):1148-53
references.pmid: 11422034
references.citation: J Immunol. 2009 Mar 15;182(6):3469-81
references.pmid: 19265125
references.citation: J Invest Dermatol. 2013 Feb;133(2):303-15
references.pmid: 22931926
references.citation: Eur Cytokine Netw. 2012 Oct-Dec;23(4):163-5
references.pmid: 23328497
references.citation: Clin Rev Allergy Immunol. 2011 Dec;41(3):298-310
references.pmid: 21249468
references.citation: Clin Immunol. 2008 Mar;126(3):332-7
references.pmid: 18166499
references.citation: Curr Opin Immunol. 2011 Dec;23(6):784-8
references.pmid: 22019285
references.citation: Curr Opin Immunol. 2009 Dec;21(6):666-78
references.pmid: 19828304
references.citation: J Invest Dermatol. 2009 Aug;129(8):1892-908
references.pmid: 19494826
references.citation: Biosci Biotechnol Biochem. 2005 Jan;69(1):19-25
references.pmid: 15665462
references.citation: Immunol Rev. 2011 Jul;242(1):233-46
references.pmid: 21682749
references.citation: Biol Pharm Bull. 2008 Jan;31(1):51-6
references.pmid: 18175941
references.citation: Vet Dermatol. 2010 Apr;21(2):184-91
references.pmid: 20141605
references.citation: J Invest Dermatol. 2008 Aug;128(8):2117-9
references.pmid: 18305568
references.citation: J Allergy Clin Immunol. 2008 Jun;121(6):1337-43
references.pmid: 18329087
references.citation: J Invest Dermatol. 2005 Jan;124(1):119-24
references.pmid: 15654964
references.citation: Exp Dermatol. 2008 Dec;17(12):1063-72
references.pmid: 19043850
references.citation: Allergy. 2005 Jan;60(1):90-7
references.pmid: 15575937
references.citation: Biol Pharm Bull. 2004 Sep;27(9):1376-81
references.pmid: 15340222
references.citation: Allergy. 2009 Feb;64(2):265-75
references.pmid: 19178406
references.citation: Cytokine Growth Factor Rev. 2010 Dec;21(6):413-23
references.pmid: 21074482
references.citation: Exp Dermatol. 2007 Jun;16(6):513-9
references.pmid: 17518992
references.citation: Mol Vis. 2012;18:317-29
references.pmid: 22355243
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: J Pharmacol Sci. 2009 Jul;110(3):251-9
references.pmid: 19609062
references.citation: Vet Immunol Immunopathol. 2006 Dec 15;114(3-4):285-96
references.pmid: 17011637
references.citation: J Invest Dermatol. 2009 Jan;129(1):31-40
references.pmid: 19078986
references.citation: Immunity. 2009 Nov 20;31(5):787-98
references.pmid: 19879162
references.citation: Immunol Today. 1998 Aug;19(8):359-61
references.pmid: 9709503
references.citation: Allergy. 1998 Jan;53(1):51-8
references.pmid: 9491229