title: Season of ejaculate collection influences the freezability of boar spermatozoa.
abstract: The aim of this retrospective study was to evaluate whether the season of ejaculate collection influences the freezability of porcine sperm. A total of 434 ejaculates were collected from boars of six different breeds over three years (2008-2011) and throughout the four seasons of the year identified in the northern hemisphere (winter, spring, summer and autumn). The ejaculates were cryopreserved using a standard 0.5 mL straw freezing protocol. Sperm quality was assessed before (fresh semen samples kept 24h at 17°C) and after freezing and thawing (at 30 and 150 min post-thawing in semen samples kept in a water bath at 37 °C), according to the percentages of total motility, as assessed by the CASA system, and viability, as assessed by flow cytometry after staining with SYBR-14, PI and PE-PNA. The data, in percentages, on sperm motility and viability after freezing and thawing were obtained at each evaluation time (recovered) and were normalized to the values before freezing (normalized). The season of ejaculate collection influenced (P<0.01) sperm quality before freezing and after thawing (recovered and normalized), irrespective of the breed of boar. Sperm quality was lower in summer, both in terms of motility and viability, and in autumn, in terms of motility, than in winter and spring. Seasonality in the normalized data indicates that the season of ejaculate collection influences sperm freezability, regardless of the season's influence on sperm quality before freezing. Consequently, the spermatozoa from ejaculates collected during summer and, to a lesser extent, also in autumn, are more sensitive to cryopreservation than those from ejaculates collected during winter and spring.
authors.affiliation: Department of Medicine and Animal Surgery, Veterinary Science, University of Murcia, 30100 Murcia, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Barranco
authors.forename: Maria D
authors.initials: MD
authors.lastname: Ortega
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Martinez-Alborcia
authors.forename: Juan M
authors.initials: JM
authors.lastname: Vazquez
authors.forename: Emilio A
authors.initials: EA
authors.lastname: Martinez
authors.forename: Jordi
authors.initials: J
authors.lastname: Roca
mesh_terms: D000818:Animals; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D005434:Flow Cytometry; D008297:Male; D009930:Organic Chemicals / Q000032:analysis; D012621:Seasons; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013048:Specimen Handling / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology*; D013552:Swine
chemical_list: D009930:Organic Chemicals; C093207:SYBR-14
keywords: Cryopreservation; Porcine; Seasonality; Sperm