title: Microarray analyses and comparisons of upper or lower flanks of rice shoot base preceding gravitropic bending.
abstract: Gravitropism is a complex process involving a series of physiological pathways. Despite ongoing research, gravitropism sensing and response mechanisms are not well understood. To identify the key transcripts and corresponding pathways in gravitropism, a whole-genome microarray approach was used to analyze transcript abundance in the shoot base of rice (Oryza sativa sp. japonica) at 0.5 h and 6 h after gravistimulation by horizontal reorientation. Between upper and lower flanks of the shoot base, 167 transcripts at 0.5 h and 1202 transcripts at 6 h were discovered to be significantly different in abundance by 2-fold. Among these transcripts, 48 were found to be changed both at 0.5 h and 6 h, while 119 transcripts were only changed at 0.5 h and 1154 transcripts were changed at 6 h in association with gravitropism. MapMan and PageMan analyses were used to identify transcripts significantly changed in abundance. The asymmetric regulation of transcripts related to phytohormones, signaling, RNA transcription, metabolism and cell wall-related categories between upper and lower flanks were demonstrated. Potential roles of the identified transcripts in gravitropism are discussed. Our results suggest that the induction of asymmetrical transcription, likely as a consequence of gravitropic reorientation, precedes gravitropic bending in the rice shoot base.
authors.affiliation: Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Graduate School of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Hu
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Mei
authors.forename: Aiping
authors.initials: A
authors.lastname: Zang
authors.forename: Haiying
authors.initials: H
authors.lastname: Chen
authors.forename: Xianying
authors.initials: X
authors.lastname: Dou
authors.forename: Jing
authors.initials: J
authors.lastname: Jin
authors.forename: Weiming
authors.initials: W
authors.lastname: Cai
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D018522:Gravitropism / Q000235:genetics* / Q000502:physiology*; D007210:Indoleacetic Acids / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics*; D018520:Plant Shoots / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D018749:RNA, Plant / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D007210:Indoleacetic Acids; D012333:RNA, Messenger; D018749:RNA, Plant
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