title: Determination of Prostaglandin E1 in dog plasma using liquid chromatography-tandem mass spectrometry and its application to a pharmacokinetic study.
abstract: The determination of Prostaglandin (PG) E1 in plasma is challenged by its low concentration (pg/mL) and endogenous interference. An LC-MS/MS method for the determination of PGE1 in dog plasma has been developed and validated. Plasma being sampled at 4°C and treated with indomethacin effectively inhibited interferents synthesized post-sampling. Samples were subjected to one-step extraction and separated by reversed phase HPLC with a short cycle time of 3min. An LLOQ of 10pg/mL was achieved with 500μl plasma. The method was applied to a pharmacokinetic study in beagle dogs involving an intravenous infusion of 3.2μg/kg PGE1. The half-life was recovered at 7min. The simple, sensitive and rapid method was suitable to be applied to pharmacokinetic studies of PGE1 at clinically relevant doses.
authors.affiliation: College of Life Science, Jilin University, 2699 Qianjin Street, Changchun 130012, PR China.
authors.forename: Yunhui
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yantong
authors.initials: Y
authors.lastname: Sun
authors.forename: Guoqing
authors.initials: G
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Yin
authors.forename: Tingting
authors.initials: T
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Jingkai
authors.initials: J
authors.lastname: Gu
mesh_terms: D000527:Alprostadil / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs / Q000097:blood*; D005260:Female; D016014:Linear Models; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000527:Alprostadil
keywords: Beagle dog; LC–MS/MS; Pharmacokinetics; Plasma; Prostaglandin E(1)