title: LRRC45 is a centrosome linker component required for centrosome cohesion.
abstract: During interphase, centrosomes are connected by a proteinaceous linker between the proximal ends of the centrioles, which is important for the centrosomes to function as a single microtubule-organizing center. However, the composition and regulation of centrosomal linker remain largely unknown. Here, we show that LRRC45 is a centrosome linker that localizes at the proximal ends of the centrioles and forms fiber-like structures between them. Depletion of LRRC45 results in centrosome splitting during interphase. Moreover, LRRC45 interacts with both C-Nap1 and rootletin and is phosphorylated by Nek2A at S661 during mitosis. After phosphorylation, both LRRC45 centrosomal localization and fiber-like structures are significantly reduced, which subsequently leads to centrosome separation. Thus, LRRC45 is a critical component of the proteinaceous linker between two centrioles and is required for centrosome cohesion.
authors.affiliation: Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State, Key Laboratory of Bio-membrane and Membrane Bio-engineering, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Runsheng
authors.initials: R
authors.lastname: He
authors.forename: Ning
authors.initials: N
authors.lastname: Huang
authors.forename: Yitian
authors.initials: Y
authors.lastname: Bao
authors.forename: Haining
authors.initials: H
authors.lastname: Zhou
authors.forename: Junlin
authors.initials: J
authors.lastname: Teng
authors.forename: Jianguo
authors.initials: J
authors.lastname: Chen
mesh_terms: D018385:Centrosome / Q000378:metabolism* / Q000502:physiology; D003598:Cytoskeletal Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007399:Interphase / Q000502:physiology; D000072379:NIMA-Related Kinases; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism
chemical_list: C499237:CROCC protein, human; D003598:Cytoskeletal Proteins; C480698:LRRC4C protein, human; D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; C089441:NEK2 protein, human; D000072379:NIMA-Related Kinases; D017346:Protein Serine-Threonine Kinases