title: Lhx3-Chx10 reticulospinal neurons in locomotor circuits.
abstract: Motor behaviors result from the interplay between the brain and the spinal cord. Reticulospinal neurons, situated between the supraspinal structures that initiate motor movements and the spinal cord that executes them, play key integrative roles in these behaviors. However, the molecular identities of mammalian reticular formation neurons that mediate motor behaviors have not yet been determined, thus limiting their study in health and disease. In the medullary reticular formation of the mouse, we identified neurons that express the transcription factors Lhx3 and/or Chx10, and demonstrate that these neurons form a significant component of glutamatergic reticulospinal pathways. Lhx3-positive medullary reticular formation neurons express Fos following a locomotor task in the adult, indicating that they are active during walking. Furthermore, they receive functional inputs from the mesencephalic locomotor region and have electrophysiological properties to support tonic repetitive firing, both of which are necessary for neurons that mediate the descending command for locomotion. Together, these results suggest that Lhx3/Chx10 medullary reticular formation neurons are involved in locomotion.
authors.affiliation: Centre de Recherche du CHU de Québec, CHUL-Neurosciences, Québec City, Québec G1V 4G2, Canada, Département de Psychiatrie et Neurosciences, Université Laval, Québec City, Québec G1K 7P4, Canada, Department of Medical Neuroscience, Dalhousie University, Halifax, Nova Scotia B3H 4R2, Canada, and Department of Surgery (Neurosurgery), Dalhousie University, Halifax, Nova Scotia B3H 4R2, Canada.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Bretzner
authors.forename: Robert M
authors.initials: RM
authors.lastname: Brownstone
mesh_terms: D000200:Action Potentials / Q000235:genetics; D000818:Animals; D000831:Animals, Newborn; D001710:Biotin / Q000031:analogs & derivatives / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D003911:Dextrans / Q000378:metabolism; D005260:Female; D007839:Functional Laterality / Q000502:physiology; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D066298:In Vitro Techniques; D060850:LIM-Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D008124:Locomotion / Q000502:physiology*; D008164:Luminescent Proteins / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009434:Neural Pathways / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D016761:Oncogene Proteins v-fos / Q000378:metabolism; D012154:Reticular Formation / Q000166:cytology* / Q000502:physiology; D012235:Rhodamines / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D003911:Dextrans; D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; C094898:Lhx3 protein; D008164:Luminescent Proteins; D009419:Nerve Tissue Proteins; D016761:Oncogene Proteins v-fos; D012235:Rhodamines; D014157:Transcription Factors; C088975:Vsx2 protein, mouse; C076397:biotinylated dextran amine; C005358:tetramethylrhodamine; D001710:Biotin; D002118:Calcium
references.citation: J Neurosci. 1988 Jun;8(6):2074-86
references.pmid: 2898514
references.citation: Neuron. 2000 Oct;28(1):41-51
references.pmid: 11086982
references.citation: Brain Res. 1977 Jan 14;120(1):173-8
references.pmid: 832116
references.citation: Prog Brain Res. 1999;123:285-94
references.pmid: 10635724
references.citation: Annu Rev Neurosci. 2006;29:279-306
references.pmid: 16776587
references.citation: J Neurosci. 2005 Aug 10;25(32):7401-5
references.pmid: 16093391
references.citation: Neuroscience. 2003;121(3):731-46
references.pmid: 14568032
references.citation: Brain Res Brain Res Rev. 2002 Oct;40(1-3):166-77
references.pmid: 12589915
references.citation: Biofizika. 1970 Jan-Feb;15(1):171-8
references.pmid: 5457895
references.citation: Brain. 2007 Jun;130(Pt 6):1596-607
references.pmid: 17251240
references.citation: Prog Neurobiol. 1987;28(2):161-95
references.pmid: 3544055
references.citation: Proc Natl Acad Sci U S A. 2004 Oct 26;101(43):15488-93
references.pmid: 15494439
references.citation: Neuroscience. 2002;113(1):65-77
references.pmid: 12123685
references.citation: J Physiol. 2008 Nov 1;586(21):5259-76
references.pmid: 18772205
references.citation: Brain Res Brain Res Rev. 2002 Oct;40(1-3):178-91
references.pmid: 12589916
references.citation: Brain Res. 1987 Jan 27;402(1):78-86
references.pmid: 3828790
references.citation: Dev Biol. 2002 Sep 1;249(1):30-43
references.pmid: 12217316
references.citation: J Neurosci. 2010 Nov 3;30(44):14883-95
references.pmid: 21048147
references.citation: Eur J Neurosci. 2008 Nov;28(9):1795-807
references.pmid: 18973595
references.citation: J Neurophysiol. 2005 Jul;94(1):657-72
references.pmid: 15788518
references.citation: J Neurosci. 1993 Mar;13(3):990-1000
references.pmid: 8095068
references.citation: Brain Res Rev. 2008 Jan;57(1):183-91
references.pmid: 17928060
references.citation: Science. 1974 Jul 12;185(4146):181-3
references.pmid: 4834220
references.citation: Exp Brain Res. 1989;74(1):11-23
references.pmid: 2924828
references.citation: Prog Neurobiol. 2011 Feb;93(2):244-69
references.pmid: 21093529
references.citation: Neuron. 2001 Feb;29(2):367-84
references.pmid: 11239429
references.citation: J Neurophysiol. 2005 Jun;93(6):3442-52
references.pmid: 15634712
references.citation: J Neurophysiol. 2004 Oct;92(4):2217-38
references.pmid: 15175364
references.citation: Br J Neurosurg. 2008;22 Suppl 1:S41-4
references.pmid: 19085352
references.citation: Curr Biol. 2013 May 20;23(10):843-9
references.pmid: 23623549
references.citation: Mol Cell Neurosci. 2005 Jan;28(1):30-41
references.pmid: 15607939
references.citation: Dev Biol. 2008 Oct 15;322(2):263-75
references.pmid: 18680739
references.citation: J Neurophysiol. 2003 Sep;90(3):1714-27
references.pmid: 12736238
references.citation: J Neurophysiol. 1978 Nov;41(6):1580-91
references.pmid: 731291
references.citation: Prog Brain Res. 2004;143:221-30
references.pmid: 14653167
references.citation: Ann N Y Acad Sci. 1998 Nov 16;860:94-105
references.pmid: 9928304
references.citation: Nat Rev Neurosci. 2009 Jul;10(7):507-18
references.pmid: 19543221
references.citation: J Neurophysiol. 1998 Dec;80(6):3284-96
references.pmid: 9862922
references.citation: Neuroscientist. 2006 Feb;12(1):67-79
references.pmid: 16394194
references.citation: Brain Res. 1984 Jul 30;307(1-2):263-76
references.pmid: 6466996
references.citation: J Neurosci. 2011 Mar 23;31(12):4731-42
references.pmid: 21430172
references.citation: Eur J Neurosci. 2007 Dec;26(11):3003-15
references.pmid: 18028108
references.citation: Proc Natl Acad Sci U S A. 2012 Jan 10;109(2):E84-92
references.pmid: 22160700
references.citation: Cell. 1998 Dec 11;95(6):817-28
references.pmid: 9865699
references.citation: J Neurophysiol. 2010 Apr;103(4):1712-27
references.pmid: 20164390
references.citation: Neuron. 2004 May 13;42(3):375-86
references.pmid: 15134635
references.citation: J Neurophysiol. 2005 Aug;94(2):1392-404
references.pmid: 15872068
references.citation: Nat Neurosci. 2010 Oct;13(10):1190-8
references.pmid: 20818386
references.citation: Neurosci Biobehav Rev. 1997 Sep;21(5):631-47
references.pmid: 9353796
references.citation: J Neurophysiol. 2001 Feb;85(2):679-98
references.pmid: 11160503
references.citation: Brain Res. 1985 Mar 18;330(1):43-54
references.pmid: 3986540
references.citation: J Neurophysiol. 2003 Sep;90(3):1464-78
references.pmid: 12634275
references.citation: J Physiol. 2011 Dec 1;589(Pt 23):5603-12
references.pmid: 21878519
references.citation: Neuroscience. 2003;119(1):293-308
references.pmid: 12763089
references.citation: Nat Neurosci. 2010 Feb;13(2):246-52
references.pmid: 20081850
references.citation: Brain Res. 1988 Jun 14;452(1-2):273-85
references.pmid: 3401734
references.citation: Brain Res. 1987 May 12;411(1):1-12
references.pmid: 2440511
references.citation: Brain Res Bull. 1987 Jun;18(6):731-8
references.pmid: 3304544
references.citation: Brain Res Rev. 2008 Jan;57(1):172-82
references.pmid: 17916380
references.citation: Brain Res Rev. 2008 Jan;57(1):22-8
references.pmid: 17825424
references.citation: J Neurophysiol. 1982 Dec;48(6):1416-32
references.pmid: 7153799
references.citation: Curr Opin Neurobiol. 2011 Feb;21(1):110-5
references.pmid: 20970321
references.citation: J Neurophysiol. 2010 Jul;104(1):366-81
references.pmid: 20393059
references.citation: J Physiol. 2006 Apr 15;572(Pt 2):443-58
references.pmid: 16469789
references.citation: Brain Res Bull. 1990 Mar;24(3):499-508
references.pmid: 1970947
references.citation: Prog Brain Res. 2004;143:77-95
references.pmid: 14653153
references.citation: Biofizika. 1966;11(4):659-66
references.pmid: 6000625
references.citation: J Neurosci. 2012 Jun 6;32(23):7895-906
references.pmid: 22674265
references.citation: Annu Rev Physiol. 2007;69:291-316
references.pmid: 17009928
references.citation: Neurosci Res. 1995 Aug;23(1):89-102
references.pmid: 7501304
references.citation: Curr Opin Neurobiol. 1993 Dec;3(6):982-8
references.pmid: 8124083
references.citation: Prog Brain Res. 2010;187:81-95
references.pmid: 21111202
references.citation: J Neurophysiol. 1995 Oct;74(4):1404-20
references.pmid: 8989381
references.citation: Neuroscience. 1991;45(3):753-9
references.pmid: 1775247
references.citation: Exp Brain Res. 1984;56(2):257-62
references.pmid: 6479262
references.citation: Brain. 2012 Jul;135(Pt 7):2277-89
references.pmid: 22581799
references.citation: Eur J Neurosci. 2007 Dec;26(11):2989-3002
references.pmid: 18028107
references.citation: J Neurosci. 2012 Jun 13;32(24):8208-18
references.pmid: 22699902
references.citation: Brain Res. 1987 May 12;411(1):13-20
references.pmid: 3607422
references.citation: Neuroscience. 2007 Feb 9;144(3):1120-32
references.pmid: 17137720
references.citation: Prog Brain Res. 2004;143:251-61
references.pmid: 14653170
references.citation: J Physiol. 2005 Jul 15;566(Pt 2):519-32
references.pmid: 15878947
references.citation: J Neurosci. 2010 Jan 6;30(1):24-37
references.pmid: 20053884
references.citation: J Neurophysiol. 2006 Nov;96(5):2229-52
references.pmid: 16837662
references.citation: J Neurosci Methods. 2004 Oct 15;139(1):33-41
references.pmid: 15351519
references.citation: Nat Neurosci. 2010 Mar;13(3):333-7
references.pmid: 20154682
references.citation: Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1164-9
references.pmid: 21199947
references.citation: Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1170-5
references.pmid: 21199937
references.citation: J Neurophysiol. 1985 Nov;54(5):1101-8
references.pmid: 4078609
references.citation: J Neurophysiol. 1980 Nov;44(5):849-61
references.pmid: 6255109
references.citation: Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7319-24
references.pmid: 12777621
references.citation: J Appl Physiol (1985). 2006 Mar;100(3):1077-9
references.pmid: 16467394
references.citation: Nature. 2003 Oct 30;425(6961):917-25
references.pmid: 14586460
references.citation: Exp Brain Res. 1991;85(3):674-81
references.pmid: 1655510
references.citation: J Neurophysiol. 2007 Apr;97(4):3118-25
references.pmid: 17303810
references.citation: J Neurosci. 1995 Jan;15(1 Pt 1):342-58
references.pmid: 7823140
references.citation: J Neurosci. 2010 Sep 15;30(37):12274-80
references.pmid: 20844123
references.citation: J Neurosci. 1991 Jun;11(6):1691-700
references.pmid: 2045881
references.citation: J Neurosci Res. 2010 Oct;88(13):2833-46
references.pmid: 20568293
references.citation: Nat Neurosci. 2001 Nov;4 Suppl:1183-91
references.pmid: 11687828
references.citation: Development. 2000 Sep;127(17):3829-38
references.pmid: 10934027
references.citation: Neurosci Res. 1995 Aug;23(1):47-53
references.pmid: 7501300
references.citation: Nat Neurosci. 2010 Sep;13(9):1066-74
references.pmid: 20680010
references.citation: J Neurosci. 2010 Jan 20;30(3):1137-48
references.pmid: 20089922
references.citation: Brain Res. 2008 Jul 30;1222:42-50
references.pmid: 18586225
references.citation: Neuron. 2011 Sep 22;71(6):1071-84
references.pmid: 21943604
references.citation: Ann N Y Acad Sci. 1998 Nov 16;860:83-93
references.pmid: 9928303
references.citation: J Neurosci. 2005 Nov 23;25(47):10863-73
references.pmid: 16306399
references.citation: Proc Natl Acad Sci U S A. 2005 Jul 26;102(30):10688-93
references.pmid: 16020526
references.citation: J Neurosci. 2005 Jun 15;25(24):5710-9
references.pmid: 15958737