title: Periodontal regeneration using periodontal ligament stem cell-transferred amnion.
abstract: Periodontal disease is characterized by the destruction of tooth supporting tissues. Regeneration of periodontal tissues using ex vivo expanded cells has been introduced and studied, although appropriate methodology has not yet been established. We developed a novel cell transplant method for periodontal regeneration using periodontal ligament stem cell (PDLSC)-transferred amniotic membrane (PDLSC-amnion). The aim of this study was to investigate the regenerative potential of PDLSC-amnion in a rat periodontal defect model. Cultured PDLSCs were transferred onto amniotic membranes using a glass substrate treated with polyethylene glycol and photolithography. The properties of PDLSCs were investigated by flow cytometry and in vitro differentiation. PDLSC-amnion was transplanted into surgically created periodontal defects in rat maxillary molars. Periodontal regeneration was evaluated by microcomputed tomography (micro-CT) and histological analysis. PDLSCs showed mesenchymal stem cell-like characteristics such as cell surface marker expression (CD90, CD44, CD73, CD105, CD146, and STRO-1) and trilineage differentiation ability (i.e., into osteoblasts, adipocytes, and chondrocytes). PDLSC-amnion exhibited a single layer of PDLSCs on the amniotic membrane and stability of the sheet even with movement and deformation caused by surgical instruments. We observed that the PDLSC-amnion enhanced periodontal tissue regeneration as determined by micro-CT and histology by 4 weeks after transplantation. These data suggest that PDLSC-amnion has therapeutic potential as a novel cell-based regenerative periodontal therapy.
authors.affiliation: 1 Department of Nanomedicine (DNP), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University , Tokyo, Japan .
authors.forename: Kengo
authors.initials: K
authors.lastname: Iwasaki
authors.forename: Motohiro
authors.initials: M
authors.lastname: Komaki
authors.forename: Naoki
authors.initials: N
authors.lastname: Yokoyama
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Atsuko
authors.initials: A
authors.lastname: Taki
authors.forename: Izumi
authors.initials: I
authors.lastname: Honda
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Kimura
authors.forename: Masaki
authors.initials: M
authors.lastname: Takeda
authors.forename: Keiko
authors.initials: K
authors.lastname: Akazawa
authors.forename: Shigeru
authors.initials: S
authors.lastname: Oda
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Izumi
authors.forename: Ikuo
authors.initials: I
authors.lastname: Morita
mesh_terms: D000293:Adolescent; D000328:Adult; D000650:Amnion / Q000637:transplantation*; D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D008297:Male; D008437:Maxilla / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery; D059630:Mesenchymal Stem Cells / Q000166:cytology; D010513:Periodontal Ligament / Q000000981:diagnostic imaging / Q000502:physiology*; D051381:Rats; D011916:Rats, Inbred F344; D011923:Rats, Nude; D012038:Regeneration; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D014057:Tomography, X-Ray Computed; D055815:Young Adult
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