title: Development of UV/HPLC methods for quantitative analysis of benznidazole in human plasma and urine for application in pediatric clinical studies.
abstract: OBJECTIVE
  Chagas disease constitutes a major public health problem in Latin America. Correctly designed pharmacokinetic, safety, and bioequivalence studies are desirable in order to fill the knowledge gaps that presently exist on available drugs. It is necessary to develop accurate, simple, reproducible, and sensitive high-performance liquid chromatography (HPLC)/UV methods for the quantization of benznidazole (BNZ) in human plasma and urine for clinical applications, specially in pediatric patients.
  
  
  METHODS
  Quantization of BNZ in human plasma involved freeze-drying and re-suspension in organic solvent followed by reverse phase HPLC with UV detection. Analysis of BNZ in urine involved liquid/liquid extraction followed by reverse phase HPLC with UV detection.
  
  
  RESULTS
  Limits of quantization (LOQ) were 0.32 μg/ml for plasma and 5.2 μg/ml for urine. No metabolite interferences were showed in both methods.
  
  
  CONCLUSIONS
  The LOQ of methods seems appropriate in pediatric clinical contexts. Both procedures were applied with good results, to the quantization of BNZ in plasma and urine of patients treated for Chagas disease.
authors.affiliation: Área de Toxicología, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata. La Plata, Provincia de Buenos Aires, Argentina; Laboratorio de Servicios a la Industria y al Sistema Científico (LaSeISiC), Universidad Nacional de La Plata / Comisión de Investigaciones Científicas de la Provincia de Buenos Aires, Argentina.
authors.forename: María Elena
authors.initials: ME
authors.lastname: Marsón
authors.forename: Diego Dante
authors.initials: DD
authors.lastname: Dana
authors.forename: Jaime
authors.initials: J
authors.lastname: Altcheh
authors.forename: Facundo
authors.initials: F
authors.lastname: García-Bournissen
authors.forename: Guido
authors.initials: G
authors.lastname: Mastrantonio
mesh_terms: D014355:Chagas Disease / Q000188:drug therapy; D002648:Child; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005612:Freeze Drying; D006801:Humans; D009593:Nitroimidazoles / Q000097:blood* / Q000627:therapeutic use / Q000652:urine*; D013048:Specimen Handling / Q000379:methods; D014344:Trypanocidal Agents / Q000097:blood / Q000627:therapeutic use / Q000652:urine
chemical_list: D009593:Nitroimidazoles; D014344:Trypanocidal Agents; C009999:benzonidazole
keywords: Benznidazole; Chagas Disease; HPLC; Plasma; Urine
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