title: Structural insights into incorporation of norbornene amino acids for click modification of proteins.
abstract: Three for two: by using a Methanosarcina mazei PylRS triple mutant (Y306G, Y384F, I405R) the incorporation of two new exo-norbornene-containing pyrrolysine analogues was achieved. X-ray crystallographic analysis led to the identification of the crucial structural elements involved in substrate recognition by the evolved synthetase.
authors.affiliation: Department of Chemistry, TU Munich, Lichtenbergstrasse 4, 85748 Garching (Germany). sabine.schneider@mytum.de.
authors.forename: Sabine
authors.initials: S
authors.lastname: Schneider
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Gattner
authors.forename: Milan
authors.initials: M
authors.lastname: Vrabel
authors.forename: Veronika
authors.initials: V
authors.lastname: Flügel
authors.forename: Verónica
authors.initials: V
authors.lastname: López-Carrillo
authors.forename: Stefan
authors.initials: S
authors.lastname: Prill
authors.forename: Thomas
authors.initials: T
authors.lastname: Carell
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism*; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics / Q000378:metabolism*; D057930:Click Chemistry; D018360:Crystallography, X-Ray; D008239:Lysine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D017020:Methanosarcina / Q000201:enzymology; D009154:Mutation; D009636:Norbornanes / Q000737:chemistry*; D017434:Protein Structure, Tertiary
chemical_list: D000596:Amino Acids; D009636:Norbornanes; D000604:Amino Acyl-tRNA Synthetases; C456839:pyrrolysine; D008239:Lysine
keywords: X-ray structures; click chemistry; norbornenes; protein modifications; pyrrolysine