title: Altered expression of caveolin 2 and 3 in smooth muscle of rat urinary bladder by 17β-estradiol.
abstract: BACKGROUND
  The purpose of this study was to investigate the effect of estrogen alteration on the expression of caveolin 2 and 3 in rat smooth muscle of urinary bladder.
  
  
  METHODS
  Female Sprague-Dawley rats were divided into three groups: control, bilateral ovariectomy (Ovx), and bilateral ovariectomy followed by subcutaneous injections of 17β-estradiol (Ovx+Est). After 4 weeks, urodynamic measurements were taken to ascertain the contraction interval and contraction pressure. The expression and cellular localization of caveolin 2 and 3 were determined by Western blot and immunohistochemistry in rat urinary bladder smooth muscle.
  
  
  RESULTS
  In cystometrograms, the contraction interval (min) was significantly lower in the Ovx group (3.1±1.5) than in the control group (5.6±1.2), but was increased after estrogen treatment (9.3±1.0). Conversely, the average contraction pressure (mmHg) was higher in the Ovx group (26.2±2.3) than in the control group (21.9±3.1), and was decreased after estrogen treatment (23.8±3.5). Caveolin 2 and 3 expression was localized in the cell membrane of the smooth muscle. The protein expression of both caveolin 2 and 3 was significantly lower after ovariectomy and was restored to the control levels after 17β-estradiol treatment.
  
  
  CONCLUSIONS
  Hormonal alteration causes a significant change in the expression of caveolin 2 and 3 in smooth muscle of rat urinary bladder. These findings suggest that these molecules might have functional roles in the detrusor overactivity that occurs in association with hormonal alteration.
authors.affiliation: Department of Urology, Research Institute of Medical Sciences, Chonnam National University, 8, Hak-dong, Dong-ku, Gwangju 501-757, Korea. urokwon@gmail.com.
authors.forename: Sun-Ouck
authors.initials: SO
authors.lastname: Kim
authors.forename: Seung Hee
authors.initials: SH
authors.lastname: Song
authors.forename: Seung-Chul
authors.initials: SC
authors.lastname: Lee
authors.forename: Kyung Aa
authors.initials: KA
authors.lastname: Cho
authors.forename: Jong Sung
authors.initials: JS
authors.lastname: Park
authors.forename: Dongdeuk
authors.initials: D
authors.lastname: Kwon
authors.forename: Kwangsung
authors.initials: K
authors.lastname: Park
mesh_terms: D000818:Animals; D051243:Caveolin 2 / Q000378:metabolism*; D051244:Caveolin 3 / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism*; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D001743:Urinary Bladder / Q000187:drug effects / Q000378:metabolism*
chemical_list: C495546:Cav2 protein, rat; C495549:Cav3 protein, rat; D051243:Caveolin 2; D051244:Caveolin 3; D004958:Estradiol
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