title: Finding the best antibody dilution by repeated immunostaining of the same tissue section.
abstract: One can find the optimal antibody dilution for immunostaining by repeated staining on the same tissue section by using a less dilute antibody for each attempt. Using secondary antibody and horseradish peroxidase conjugated to a dextran polymer, a section stained repeatedly with several dilutions of antibody appears as good as a section stained with only the last dilution.
authors.affiliation: Barry University School of Podiatric Medicine , Miami Shores , Florida.
authors.forename: A A
authors.initials: AA
authors.lastname: Smith
mesh_terms: D000906:Antibodies / Q000276:immunology*; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D007201:Indicator Dilution Techniques
chemical_list: D000906:Antibodies; D006735:Horseradish Peroxidase