title: Effect of pretreatment on saccharification of sugarcane bagasse by complex and simple enzyme mixtures.
abstract: Saccharification of sugarcane bagasse pretreated at the pilot-scale with different processes (in combination with steam-explosion) was evaluated. Maximum glucan conversion with Celluclast 1.5L (15-25FPU/g glucan) was in the following order: glycerol/HCl>HCl>H2SO4>NaOH, with the glycerol system achieving ≈ 100% conversion. Surprisingly, the NaOH substrate achieved optimum saccharification with only 8 FPU/g glucan. Glucan conversions (3.6-6%) obtained with mixtures of endo-1,4-β-glucanase (EG) and β-glucosidase (βG) for the NaOH substrate were 2-6 times that of acid substrates. However, glucan conversions (15-60%) obtained with mixtures of cellobiohydrolase (CBH I) and βG on acidified glycerol substrate were 10-30% higher than those obtained for NaOH and acid substrates. The susceptibility of the substrates to enzymatic saccharification was explained by their physical and chemical attributes. Acidified glycerol pretreatment offers the opportunity to simplify the complexity of enzyme mixtures required for saccharification of lignocellulosics.
authors.affiliation: Syngenta Centre for Sugarcane Biofuels Development, Centre for Tropical Crops and Biocommodities, Queensland University of Technology, GP.O. Box 2432, 2 George Street, Brisbane, Queensland 4001, Australia; Centre for Tropical Crops and Biocommodities, Queensland University of Technology, GP.O. Box 2432, 2 George Street, Brisbane, Queensland 4001, Australia. Electronic address: md.harrison@qut.edu.au.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Harrison
authors.forename: Zhanying
authors.initials: Z
authors.lastname: Zhang
authors.forename: Kylie
authors.initials: K
authors.lastname: Shand
authors.forename: Ian M
authors.initials: IM
authors.lastname: O'Hara
authors.forename: William O S
authors.initials: WO
authors.lastname: Doherty
authors.forename: James L
authors.initials: JL
authors.lastname: Dale
mesh_terms: D018533:Biomass; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D002480:Cellulase / Q000494:pharmacology*; D002482:Cellulose / Q000378:metabolism*; D006868:Hydrolysis; D008855:Microscopy, Electron, Scanning; D010865:Pilot Projects; D011994:Recombinant Proteins / Q000494:pharmacology; D031786:Saccharum / Q000187:drug effects / Q000378:metabolism*; D012972:Sodium Hydroxide / Q000494:pharmacology; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared; D013227:Steam; D013464:Sulfuric Acids / Q000494:pharmacology; D014961:X-Ray Diffraction; D001617:beta-Glucosidase / Q000494:pharmacology*
chemical_list: D011994:Recombinant Proteins; D012996:Solutions; D013227:Steam; D013464:Sulfuric Acids; D012972:Sodium Hydroxide; D002482:Cellulose; C027433:bagasse; D001617:beta-Glucosidase; D002480:Cellulase; C033158:sulfuric acid
keywords: Bagasse; Cellulase; Glycerol; Pretreatment; Sugarcane