title: Effect of organic matter strength on anammox for modified greenhouse turtle breeding wastewater treatment.
abstract: Anaerobic ammonium-N removal from modified greenhouse turtle breeding wastewater with different chemical oxygen demand (COD) strengths (194.0-577.8 mg L(-1)) at relatively fixed C/N ratios (≈ 2) was investigated using a lab-scale up-flow anaerobic sludge blanket (UASB) anammox reactor. During the entire experiment, the total nitrogen (TN) removal efficiency was about 85% or higher, while the average COD removal efficiency was around 56.5 ± 7.9%. Based on the nitrogen and carbon balance, the nitrogen removal contribution was 79.6 ± 4.2% for anammox, 12.7 ± 3.0% for denitrification+denitritation and 7.7 ± 4.9% for other mechanisms. Denaturing gradient gel electrophoresis (DGGE) analyses revealed that Planctomycete, Proteobacteria and Chloroflexi bacteria were coexisted in the reactor. Anammox was always dominant when the reactor was fed with different COD concentrations, which indicated the stability of the anammox process with the coexistence of the denitrification process in treating greenhouse turtle breeding wastewater.
authors.affiliation: Institute of Environmental Science and Technology, Zhejiang University, Hangzhou 310058, PR China; School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, PR China.
authors.forename: Chongjun
authors.initials: C
authors.lastname: Chen
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Huang
authors.forename: Chenxiao
authors.initials: C
authors.lastname: Lei
authors.forename: Tian C
authors.initials: TC
authors.lastname: Zhang
authors.forename: Weixiang
authors.initials: W
authors.lastname: Wu
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D000693:Anaerobiosis / Q000187:drug effects; D000818:Animals; D017756:Aquaculture; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology; D001947:Breeding; D058645:Denaturing Gradient Gel Electrophoresis; D009566:Nitrates / Q000032:analysis; D009573:Nitrites / Q000032:analysis; D009584:Nitrogen / Q000302:isolation & purification; D009930:Organic Chemicals / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology; D013997:Time Factors; D014426:Turtles / Q000254:growth & development*; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000382:microbiology*; D018508:Water Purification / Q000379:methods*
chemical_list: D000641:Ammonia; D009566:Nitrates; D009573:Nitrites; D009584:Nitrogen; D009930:Organic Chemicals; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D062065:Wastewater
keywords: Anammox; Greenhouse turtle breeding wastewater; Nitrogen removal; PCR–DGGE