title: The effect of allostatic load on hypothalamic-pituitary-interrenal (HPI) axis before and after secondary vaccination in Atlantic salmon postsmolts (Salmo salar L.).
abstract: The experiment consisted of three experimental groups: (1) "vaccine and stress", (2) "stress and vaccine" and (3) control. All groups have previously been vaccinated 6 months prior to the start of the experiment. At the start of the experiment, the "vaccine and stress" group was vaccinated with Pentium Forte Plus for the second time (25.02.2008) and then given a daily stressor (confinement stressor 267 kg m-3 in 15 min) for a period of 4 weeks. The "stress and vaccine" group was given a similar daily stressor for 4 weeks and then vaccinated for the second time. The control group was neither stressed nor vaccinated a second time. The results indicates that fish in the "stress and vaccine" group may have entered an allostatic overload type 2 due to oversensitivity to ACTH, a reduced efficient negative feedback system with elevated baseline levels of plasma cortisol and reduced immune response with pronounced effects on the well-being of the animal. The "vaccine and stress" group may likewise have entered an allostatic overload type 1 response, with oversensitivity to ACTH and transient reduced efficient negative feedback system. This study shows that if plasma cortisol becomes elevated prior to vaccination, it could perhaps instigate an allostatic overload type 2 with dire consequences on animal welfare. To reduce the risk of compromising the animal welfare during commercial vaccination of salmon, one propose to grade the fish minimum a week prior to vaccination or grade simultaneously with vaccination. This could reduce the overall allostatic load during handling and vaccination and secure a healthy fish with intact immune response and improved animal welfare.
authors.affiliation: Faculty of Biosciences and Aquaculture, University of Nordland, 8049, Bodø, Norway, martin.haugmo.iversen@uin.no.
authors.forename: Martin H
authors.initials: MH
authors.lastname: Iversen
authors.forename: Robert A
authors.initials: RA
authors.lastname: Eliassen
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D048409:Aeromonas salmonicida / Q000276:immunology; D052636:Allostasis / Q000276:immunology* / Q000502:physiology*; D000827:Animal Welfare; D000818:Animals; D025461:Feedback, Physiological; D005393:Fish Diseases / Q000276:immunology / Q000517:prevention & control; D005398:Fisheries; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D006854:Hydrocortisone / Q000097:blood; D007030:Hypothalamo-Hypophyseal System / Q000276:immunology / Q000502:physiology; D007117:Immunization, Secondary / Q000379:methods / Q000662:veterinary*; D007668:Kidney / Q000276:immunology / Q000502:physiology; D009664:Norway; D064587:Osmoregulation; D020317:Salmo salar / Q000254:growth & development / Q000276:immunology* / Q000502:physiology*; D013312:Stress, Physiological
chemical_list: D000324:Adrenocorticotropic Hormone; D006854:Hydrocortisone
references.citation: Integr Comp Biol. 2002 Jul;42(3):517-25
references.pmid: 21708747
references.citation: Horm Behav. 2003 Jan;43(1):2-15
references.pmid: 12614627
references.citation: Dev Comp Immunol. 2011 Dec;35(12):1366-75
references.pmid: 21782845
references.citation: Physiol Behav. 2007 Oct 22;92(3):317-39
references.pmid: 17234221
references.citation: Fish Shellfish Immunol. 2003 Nov;15(5):397-410
references.pmid: 14550666
references.citation: Psychoneuroendocrinology. 1986;11(2):205-11
references.pmid: 3092267
references.citation: Ann N Y Acad Sci. 1998 May 1;840:33-44
references.pmid: 9629234
references.citation: Fish Physiol Biochem. 1989 Jun;7(1-6):253-8
references.pmid: 24221779
references.citation: Fish Shellfish Immunol. 2006 Jan;20(1):83-96
references.pmid: 15961320
references.citation: Gen Comp Endocrinol. 1999 Oct;116(1):122-32
references.pmid: 10525368
references.citation: J Anim Sci. 1985 May;60(5):1095-101
references.pmid: 4008357
references.citation: Physiol Rev. 1997 Jul;77(3):591-625
references.pmid: 9234959
references.citation: Fish Shellfish Immunol. 2004 Oct;17(4):375-87
references.pmid: 15312664
references.citation: Vet Immunol Immunopathol. 1999 Dec 15;72(1-2):203-12
references.pmid: 10614510
references.citation: Neurosci Biobehav Rev. 2010 Sep;35(1):2-16
references.pmid: 19822172
references.citation: Comp Biochem Physiol A Mol Integr Physiol. 2001 Jun;129(2-3):399-404
references.pmid: 11423312
references.citation: Mol Immunol. 2008 Nov;46(1):70-9
references.pmid: 18804866
references.citation: Br Med J. 1950 Jun 17;1(4667):1383-92
references.pmid: 15426759
references.citation: Fish Shellfish Immunol. 2008 Feb;24(2):194-204
references.pmid: 18065240
references.citation: J Psychiatry Neurosci. 2005 Sep;30(5):315-8
references.pmid: 16151535
references.citation: J Endocrinol. 2002 Oct;175(1):261-7
references.pmid: 12379511
references.citation: Mar Environ Res. 2010 Jul;70(1):26-34
references.pmid: 20338632
references.citation: Ann N Y Acad Sci. 2006 Jun;1069:62-76
references.pmid: 16855135
references.citation: Gen Comp Endocrinol. 2010 Feb 1;165(3):549-56
references.pmid: 19596332
references.citation: Gen Comp Endocrinol. 2006 Sep 1;148(2):195-202
references.pmid: 16677647
references.citation: Gen Comp Endocrinol. 2011 Aug 1;173(1):129-38
references.pmid: 21624369
references.citation: J Endocrinol. 2008 Feb;196(2):313-22
references.pmid: 18252954
references.citation: Eur J Endocrinol. 2008 Nov;159(5):649-52
references.pmid: 18753312
references.citation: Prev Vet Med. 2009 Jul 1;90(1-2):127-36
references.pmid: 19419787
references.citation: Psychoneuroendocrinology. 1984;9(1):45-55
references.pmid: 6739664
references.citation: Biol Psychiatry. 1986 May;21(5-6):436-44
references.pmid: 3697434
references.citation: J Anim Sci. 1994 Jul;72(7):1771-7
references.pmid: 7928757
references.citation: J Anim Sci. 1987 May;64(5):1371-7
references.pmid: 3583943
references.citation: Vet Immunol Immunopathol. 2002 Sep 10;87(3-4):467-79
references.pmid: 12072274
references.citation: J Anim Sci. 2001 Oct;79(10):2580-93
references.pmid: 11721837
references.citation: Comp Biochem Physiol B Biochem Mol Biol. 2007 Sep;148(1):32-43
references.pmid: 17544309
references.citation: J Endocrinol. 1990 Feb;124(2):311-8
references.pmid: 2155988
references.citation: Fish Shellfish Immunol. 2008 May;24(5):649-58
references.pmid: 18359641
references.citation: Perspect Biol Med. 1973 Spring;16(3):441-5
references.pmid: 4705073