title: Structural Insights on the bacteriolytic and self-protection mechanism of muramidase effector Tse3 in Pseudomonas aeruginosa.
abstract: The warfare among microbial species as well as between pathogens and hosts is fierce, complicated, and continuous. In Pseudomonas aeruginosa, the muramidase effector Tse3 (Type VI secretion exported 3) can be injected into the periplasm of neighboring bacterial competitors by a Type VI secretion apparatus, eventually leading to cell lysis and death. However, P. aeruginosa protects itself from lysis by expressing immune protein Tsi3 (Type six secretion immunity 3). Here, we report the crystal structure of the Tse3-Tsi3 complex at 1.8 Å resolution, revealing that Tse3 possesses one open accessible, goose-type lysozyme-like domain with peptidoglycan hydrolysis activity. Calcium ions bind specifically in the Tse3 active site and are identified to be crucial for its bacteriolytic activity. In combination with biochemical studies, the structural basis of self-protection mechanism of Tsi3 is also elucidated, thus providing an understanding and new insights into the effectors of Type VI secretion system.
authors.affiliation: From the Laboratory for Computational Chemistry and Drug Design and.
authors.forename: Lianbo
authors.initials: L
authors.lastname: Li
authors.affiliation: From the Laboratory for Computational Chemistry and Drug Design and.
authors.forename: Weili
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Genomics, School of Chemical Biology & Biotechnology, Peking University, Shenzhen Graduate School, Shenzhen 518055, China.
authors.forename: Qisong
authors.initials: Q
authors.lastname: Liu
authors.affiliation: From the Laboratory for Computational Chemistry and Drug Design and.
authors.forename: Yu
authors.initials: Y
authors.lastname: Gao
authors.affiliation: From the Laboratory for Computational Chemistry and Drug Design and.
authors.forename: Ying
authors.initials: Y
authors.lastname: Gao
authors.affiliation: From the Laboratory for Computational Chemistry and Drug Design and.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chemical Genomics, School of Chemical Biology & Biotechnology, Peking University, Shenzhen Graduate School, Shenzhen 518055, China. Electronic address: dzw@pkusz.edu.cn.
authors.forename: David Zhigang
authors.initials: DZ
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chemical Genomics, School of Chemical Biology & Biotechnology, Peking University, Shenzhen Graduate School, Shenzhen 518055, China. Electronic address: lizg@pkusz.edu.cn.
authors.forename: Zigang
authors.initials: Z
authors.lastname: Li
authors.affiliation: From the Laboratory for Computational Chemistry and Drug Design and. Electronic address: tau@pku.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
mesh_terms: D058947:Bacterial Secretion Systems / Q000502:physiology*; D002118:Calcium / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D033903:Periplasmic Proteins / Q000737:chemistry* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D058947:Bacterial Secretion Systems; D033903:Periplasmic Proteins; D002118:Calcium
keywords: Antitoxins; Crystal Structure; Immune Protein Tsi3; Muramidase Effector Tse3; Peptidoglycan; Protein Complexes; Pseudomonas aeruginosa; Toxins; Type VI Secretion System
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