title: Proteomic analysis profile of engineered articular cartilage with chondrogenic differentiated adipose tissue-derived stem cells loaded polyglycolic acid mesh for weight-bearing area defect repair.
abstract: The present study was designed to investigate the possibility of full-thickness defects repair in porcine articular cartilage (AC) weight-bearing area using chondrogenic differentiated autologous adipose-derived stem cells (ASCs) with a follow-up of 3 and 6 months, which is successive to our previous study on nonweight-bearing area. The isolated ASCs were seeded onto the phosphoglycerate/polylactic acid (PGA/PLA) with chondrogenic induction in vitro for 2 weeks as the experimental group prior to implantation in porcine AC defects (8 mm in diameter, deep to subchondral bone), with PGA/PLA only as control. With follow-up time being 3 and 6 months, both neo-cartilages of postimplantation integrated well with the neighboring normal cartilage and subchondral bone histologically in experimental group, whereas only fibrous tissue in control group. Immunohistochemical and toluidine blue staining confirmed similar distribution of COL II and glycosaminoglycan in the regenerated cartilage to the native one. A vivid remolding process with repair time was also witnessed in the neo-cartilage as the compressive modulus significantly increased from 70% of the normal cartilage at 3 months to nearly 90% at 6 months, which is similar to our former research. Nevertheless, differences of the regenerated cartilages still could be detected from the native one. Meanwhile, the exact mechanism involved in chondrogenic differentiation from ASCs seeded on PGA/PLA is still unknown. Therefore, proteome is resorted leading to 43 proteins differentially identified from 20 chosen two-dimensional spots, which do help us further our research on some committed factors. In conclusion, the comparison via proteome provided a thorough understanding of mechanisms implicating ASC differentiation toward chondrocytes, which is further substantiated by the present study as a perfect supplement to the former one in nonweight-bearing area.
authors.affiliation: 1 Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai, People's Republic of China .
authors.forename: Lunli
authors.initials: L
authors.lastname: Gong
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhou
authors.forename: Yaohao
authors.initials: Y
authors.lastname: Wu
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Heng
authors.initials: H
authors.lastname: Zhou
authors.forename: Fangfang
authors.initials: F
authors.lastname: Guo
authors.forename: Lei
authors.initials: L
authors.lastname: Cui
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D001696:Biomechanical Phenomena / Q000187:drug effects; D002358:Cartilage, Articular / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology / Q000601:surgery; D002454:Cell Differentiation / Q000187:drug effects*; D020219:Chondrogenesis / Q000187:drug effects*; D015180:Electrophoresis, Gel, Two-Dimensional; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D011100:Polyglycolic Acid / Q000494:pharmacology*; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D013194:Staining and Labeling; D013234:Stem Cells / Q000166:cytology*; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*; D016474:Weight-Bearing / Q000502:physiology; D014945:Wound Healing / Q000187:drug effects
chemical_list: D020543:Proteome; D011100:Polyglycolic Acid
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