title: Determination of a novel integron-located variant (blaOXA -320 ) of Class D β-lactamase in Proteus mirabilis.
abstract: Proteus mirabilis (P. mirabilis) is one of Gram-negative pathogens encountered in clinical specimens. A clinical isolate (TRP41) of P. mirabilis was isolated from a Turkish patient in Turkey. The isolate was identified using the API 32GN system and 16S rRNA gene sequencing and it was found resistant to ampicillin/sulbactam, piperacillin, tetracycline, and trimethoprim/sulfamethoxazole. This isolate was harboring a Class 1 integron gene cassette and its DNA sequence analysis revealed a novel blaOXA variant exhibiting one amino acid substitution (Asn266Ile) from blaOXA-1 . This new variant of OXA was located on Class 1 integron together with aadA1 gene encoding aminoglycoside-modifying enzymes. According to sequence records, the new variant was named as blaOXA-320 . Cassette array and size of integron were found as blaOXA-320 -aadA1 and 2086 bp, respectively. The blaOXA-320 gene is not transferable according to conjugation experiment. In this study, we report the first identification of blaOXA-320 -aadA1 gene cassette, a novel variant of Class D β-lactamase, in P. mirabilis from Turkey.
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey.
authors.forename: Aysegul Copur
authors.initials: AC
authors.lastname: Cicek
authors.forename: Azer Ozad
authors.initials: AO
authors.lastname: Duzgun
authors.forename: Aysegul
authors.initials: A
authors.lastname: Saral
authors.forename: Cemal
authors.initials: C
authors.lastname: Sandalli
mesh_terms: D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D006801:Humans; D032023:Integrons; D008969:Molecular Sequence Data; D011513:Proteus mirabilis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014421:Turkey; D014556:Urine / Q000382:microbiology; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D012336:RNA, Ribosomal, 16S; C039404:beta-lactamase OXA-2; D001618:beta-Lactamases
keywords: Class D β-lactamase; Integron; P. mirabilis; blaOXA-320 gene