title: [Effects on DNA damage and apoptosis and P53 protein expression induced by phoxim in rat bone marrow stem cells].
abstract: OBJECTIVE
  To study the DNA damage and oxidative damage and apoptosis and P53 protein expression in rat bone marrow stem cells cultured in vitro induced by phoxim.
  
  
  METHODS
  Rat bone marrow stem cells of P3 cultured in vitro were treated with phoxim of different concentrations (0, 0.2, 2 and 20 microg/L) for 24 h after 48 h cultured. The total activity of SOD, CAT and MDA content, survival rate in cell were detected by spectrophotometry,and the DNA damage were detected by single cell gel electrophoresis. The cell apoptosis were detected by flow cytometry,and the P53 protein expression were detected by Western blot.
  
  
  RESULTS
  With the 0.2 and 20 microg/L of phoxim concentrations treated for 24 h in culture media, the total activity of SOD and CAT were significantly decreased compared with the controls (P < 0.05). With the increase of phoxim concentration in culture media, the MDA contents, DNA damage, apoptosis rates and P53 protein expression of rat bone marrow stem cells was increased significantly compared with the controls (P < 0.05).
  
  
  CONCLUSIONS
  Phoxim can increase the rates of DNA damage, oxidative damage and induce apoptosis and expression of P53 in rat bone marrow stem cells, and there exists a rise tendency with the increase concentration of phoxim.
authors.affiliation: Xinxiang Medical College, Xinxiang 453003, China. yanqing1981@126.com
authors.forename: Jianguo
authors.initials: J
authors.lastname: Yan
authors.forename: Yali
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xuekun
authors.initials: X
authors.lastname: Xing
authors.forename: Fang
authors.initials: F
authors.lastname: Fang
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Zhu
authors.forename: Songtao
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects*; D005260:Female; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009946:Organothiophosphorus Compounds / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D009946:Organothiophosphorus Compounds; D016159:Tumor Suppressor Protein p53; C003135:phoxim