title: An efficient 3D cell culture method on biomimetic nanostructured grids.
abstract: Current techniques of in vitro cell cultures are able to mimic the in vivo environment only to a limited extent, as they enable cells to grow only in two dimensions. Therefore cell culture approaches should rely on scaffolds that provide support comparable to the extracellular matrix. Here we demonstrate the advantages of novel nanostructured three-dimensional grids fabricated using electro-spinning technique, as scaffolds for cultures of neoplastic cells. The results of the study show that the fibers allow for a dynamic growth of HeLa cells, which form multi-layer structures of symmetrical and spherical character. This indicates that the applied scaffolds are nontoxic and allow proper flow of oxygen, nutrients, and growth factors. In addition, grids have been proven to be useful in in situ examination of cells ultrastructure.
authors.affiliation: Department of Cell Biology, Poznan University of Medical Science, Poznan, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Wolun-Cholewa
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Langer
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Szymanowski
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Glodek
authors.forename: Anna
authors.initials: A
authors.lastname: Jankowska
authors.forename: Wojciech
authors.initials: W
authors.lastname: Warchol
authors.forename: Jerzy
authors.initials: J
authors.lastname: Langer
mesh_terms: D032701:Biomimetics / Q000379:methods*; D018929:Cell Culture Techniques; D006367:HeLa Cells; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
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