title: miR-34c enhances mouse spermatogonial stem cells differentiation by targeting Nanos2.
abstract: miRNAs are expressed in many mammalian cells, acting specific roles in regulating gene expression or mediating special mRNAs cleavage by targeting their 3'-untranslated region (3'UTR). Some miRNAs are essential and important for animal development. However, it is still unclear what the relationship is between miR-34c and mammalian spermatogonial stem cells (SSCs). We found that a conserved microRNA-34c through its target-Nanos2, regulating SSCs' differentiation in mouse. Immunohistochemistry analysis of Nanos2 and miR-34c FISH results revealed the opposite expression trends between them. Seven bioinformatics websites and programs predicted that miR-34c has interaction sites in Nanos2's 3'UTR. Dual-luciferase reporter vector and mutated dual-luciferase reporter vector analysis validated that they are interacted. After transfection miR-34c mimics into mouse SSCs, or miR-34c lentiviral vector in vitro co-cultivation with seminiferous tubules, and Western blot analysis demonstrated that miR-34c over-expression could suppress Nanos2 expression in post-transcription level. Our experiments identified that miR-34c may promote meiosis process by interacting with Nanos2 leading up-regulation of Stra8 in mouse spermatogonial stem cells.
authors.affiliation: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Key Lab for Animal Biotechnology of Agriculture Ministry of China, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Yu
authors.forename: Hailong
authors.initials: H
authors.lastname: Mu
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Niu
authors.forename: Zhili
authors.initials: Z
authors.lastname: Chu
authors.forename: Haijing
authors.initials: H
authors.lastname: Zhu
authors.forename: Jinlian
authors.initials: J
authors.lastname: Hua
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis / Q000235:genetics; D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D008540:Meiosis; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; C477341:Nanos2 protein, mouse; D016601:RNA-Binding Proteins; C102019:Stra8 protein, mouse
keywords: DIFFERENTIATION; SPERMATOGONIAL STEM CELLS (SSCs); miR-34c; NANOS2