title: Age influence on hypersensitivity pneumonitis induced in mice by exposure to Pantoea agglomerans.
abstract: Hypersensitivity pneumonitis (HP) represents the immunologically mediated lung disease induced by repeated inhalations of a wide variety of certain finely dispersed organic antigens. In susceptible subjects, these inhalations provoke a hypersensitivity reaction characterized by intense inflammation of the terminal bronchioles, the interstitium and the alveolar tree. The inflammation often organizes into granulomas and may progress to pulmonary fibrosis. Our previous work indicated that cell extract of gram-negative bacteria Pantoea agglomerans (SE-PA) causes, in young C57BL/6J mice, pulmonary changes that are very similar to the clinical manifestations of HP in men. The purpose of presented studies was to describe the response of mice immune system while exposed to SE-PA. Particular attention was paid to examine the age influence on SE-PA induced inflammation and fibrosis in lung tissue. We used 3- and 18-month-old C57BL/6J mice. Lung samples were collected from untreated mice and animals exposed to harmful agent for 7 and 28 days. HP development was monitored by histological and biochemical evaluation. Using ELISA tests, we examined concentration of pro- and anti-inflammatory cytokines in lung homogenates. Our study demonstrated again that SE-PA provokes in mice changes typical for the clinical picture of HP, and that successive stages of disease (acute, subacute and chronic) might be obtained by modulation of time exposure. Furthermore, we found that animals' age at the time of sensitization influences the nature of observed changes (cytokine expression pattern) and the final outcome (reaction intensity and scale of fibrosis).
authors.affiliation: Institute of Agricultural Medicine , Lublin , Poland .
authors.forename: Marta Kinga
authors.initials: MK
authors.lastname: Lemieszek
authors.forename: Marco
authors.initials: M
authors.lastname: Chilosi
authors.forename: Marcin
authors.initials: M
authors.lastname: Golec
authors.forename: Czesława
authors.initials: C
authors.lastname: Skórska
authors.forename: Andras
authors.initials: A
authors.lastname: Dinnyes
authors.forename: Kaveh
authors.initials: K
authors.lastname: Mashayekhi
authors.forename: Klemens
authors.initials: K
authors.lastname: Vierlinger
authors.forename: Francois
authors.initials: F
authors.lastname: Huaux
authors.forename: Matthias
authors.initials: M
authors.lastname: Wielscher
authors.forename: Manuela
authors.initials: M
authors.lastname: Hofner
authors.forename: Yousof
authors.initials: Y
authors.lastname: Yakoub
authors.forename: Chiara
authors.initials: C
authors.lastname: Pastena
authors.forename: Isabella
authors.initials: I
authors.lastname: Daniele
authors.forename: Grażyna
authors.initials: G
authors.lastname: Cholewa
authors.forename: Jolanta
authors.initials: J
authors.lastname: Sitkowska
authors.forename: Wiesława
authors.initials: W
authors.lastname: Lisowska
authors.forename: Jacek
authors.initials: J
authors.lastname: Zwoliński
authors.forename: Janusz
authors.initials: J
authors.lastname: Milanowski
authors.forename: Barbara
authors.initials: B
authors.lastname: Mackiewicz
authors.forename: Anna
authors.initials: A
authors.lastname: Góra-Florek
authors.forename: Rolf
authors.initials: R
authors.lastname: Ziesche
authors.forename: Jacek
authors.initials: J
authors.lastname: Dutkiewicz
mesh_terms: D000375:Aging / Q000276:immunology*; D000542:Alveolitis, Extrinsic Allergic / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D000818:Animals; D045424:Complex Mixtures / Q000633:toxicity*; D016207:Cytokines / Q000276:immunology; D005260:Female; D006909:Hydroxyproline / Q000276:immunology; D008168:Lung / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D020636:Pantoea
chemical_list: D045424:Complex Mixtures; D016207:Cytokines; D006909:Hydroxyproline