title: Nuclear-encoded chloroplast RNA polymerase sigma factor SIG2 activates chloroplast-encoded phycobilisome genes in a red alga, Cyanidioschyzon merolae.
abstract: The phycobilisome (PBS) is a photosynthetic light-harvesting complex in red algae, whose structural genes are separately encoded by both the nuclear and chloroplast genomes. While the expression of PBS genes in both genomes is responsive to environmental changes to modulate light-harvesting efficiency, little is known about how gene expression of the two genomes is coordinated. In this study, we focused on the four nuclear-encoded chloroplast sigma factors to understand aspects of this coordination, and found that SIG2 directs the expression of chloroplast PBS genes in the red alga Cyanidioschyzon merolae.
authors.affiliation: Chemical Resources Laboratory, Tokyo Institute of Technology, 4259-R1-29 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan; Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo, Chiba 271-8510, Japan.
authors.forename: Gaku
authors.initials: G
authors.lastname: Fujii
authors.forename: Sousuke
authors.initials: S
authors.lastname: Imamura
authors.forename: Mitsumasa
authors.initials: M
authors.lastname: Hanaoka
authors.forename: Kan
authors.initials: K
authors.lastname: Tanaka
mesh_terms: D015152:Blotting, Northern; D002736:Chloroplasts / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation; D015151:Immunoblotting; D045524:Phycobilisomes / Q000378:metabolism*; D000461:Rhodophyta / Q000235:genetics / Q000378:metabolism*; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*
chemical_list: D045524:Phycobilisomes; C413368:Sig2 sigma factor; D012808:Sigma Factor
keywords: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; Chloroplast; Cyanidioschyzon merolae; EDTA; HEPES; Light-harvesting; OD; ORF; PAGE; Phycobilisome; SD; SDS; Sigma factor; Transcriptional regulation; base pair(s); bp; ethylenediaminetetraacetic acid; kDa; kb; kilobase(s); kilodalton(s); open reading frame; optical density; polyacrylamide gel electrophoresis; sodium dodecyl sulfate; standard deviation