title: Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodology.
abstract: Clavulanic acid is a ß-lactam antibiotic which has a potent ß-lactamase inhibiting activity. In order to optimize its production by the new isolate Streptomyces DAUFPE 3060, the influence of two independent variables, temperature and soybean flour concentration, on clavulanic acid and biomass concentrations was investigated in 250 mL-Erlenmeyers according to a 2(2) central composite design. To this purpose, temperature and soybean flour (SF) concentration were varied in the ranges 26-34°C and 10-50 g/L, respectively, and the results evaluated utilizing the Response Surface Methodology. The experimental maximum production of clavulanic acid (629 mg/L) was obtained at 32°C and 40 g/L SF after 48 h, while the maximum biomass concentration (3.9 g/L) at 30°C and 50 g/L soybean flour, respectively. These values are satisfactorily close to those (640 mg/L and 3.75 g/L, respectively) predicted by the model, thereby demonstrating the validity of the mathematical approach adopted in this study.
authors.affiliation: Departamento de Tecnologia Bioquímico-Farmacêutica, Universidade de São Paulo , São Paulo, SP , Brasil.
authors.forename: Daniela A Viana
authors.initials: DA
authors.lastname: Marques
authors.forename: Márcia N Carneiro
authors.initials: MN
authors.lastname: Cunha
authors.forename: Janete M
authors.initials: JM
authors.lastname: Araújo
authors.forename: José L
authors.initials: JL
authors.lastname: Lima-Filho
authors.forename: Attilio
authors.initials: A
authors.lastname: Converti
authors.forename: Adalberto
authors.initials: A
authors.lastname: Pessoa
authors.forename: Ana L Figueiredo
authors.initials: AL
authors.lastname: Porto
keywords: Clavulanic acid; optimization; production; response surface methodology; streptomyces sp.
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