title: Activation of snap-top capped mesoporous silica nanocontainers using two near-infrared photons.
abstract: Photoactivation of "snap-top" stoppers over the pore openings of mesoporous silica nanoparticles releases intact cargo molecules from the pores. The on-command release can be stimulated by either one UV photon or two coherent near-IR photons. Two-photon activation is particularly desirable for use in biological systems because it enables good tissue penetration and precise spatial control. Stoppers were assembled by first binding photolabile coumarin-based molecules to the nanoparticle surface. Then, after the particles were loaded with cargo, bulky β-cyclodextrin (CD) molecules were noncovalently associated with the substituted coumarin molecule, blocking the pores and preventing the cargo from escaping. One-photon excitation at 376 nm or two-photon excitation at 800 nm cleaves the bond holding the coumarin to the nanopore, releasing both the CD cap and the cargo. The dynamics of both the cleavage of the cap and the cargo release was monitored using fluorescence spectroscopy. This system traps intact cargo molecules without the necessity of chemical modification, releases them with tissue-penetrating near-IR light, and has possible applications in photostimulated drug delivery.
authors.affiliation: Department of Chemistry and Biochemistry and California NanoSystems Institute, University of California , Los Angeles, California 90095-1569, United States.
authors.forename: Tania M
authors.initials: TM
authors.lastname: Guardado-Alvarez
authors.forename: Lekshmi
authors.initials: L
authors.lastname: Sudha Devi
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Russell
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Schwartz
authors.forename: Jeffrey I
authors.initials: JI
authors.lastname: Zink
mesh_terms: D003374:Coumarins / Q000737:chemistry*; D016503:Drug Delivery Systems; D007259:Infrared Rays; D053758:Nanoparticles / Q000737:chemistry / Q000528:radiation effects*; D017785:Photons; D012822:Silicon Dioxide / Q000737:chemistry / Q000528:radiation effects*; D014466:Ultraviolet Rays; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D003374:Coumarins; D047392:beta-Cyclodextrins; D012822:Silicon Dioxide; C031215:betadex
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