title: Transcription expression and clinical significance of mRNA of vascular endothelial growth factor and endostatin in liquid-based preparation specimens from patients with cervical dysplasia and carcinoma.
abstract: OBJECTIVE
  The aim of this study was to assess the diagnostic usefulness of vascular endothelial growth factor (VEGF) and endostatin mRNA in cervical specimens of patients with cervical dysplasia and carcinoma.
  
  
  METHODS
  Transcription levels of VEGF and endostatin were detected by RT-PCR in cervical liquid-based preparation specimens and compared with cytological assessments.
  
  
  RESULTS
  VEGF as well as endostatin mRNA expression was significantly associated with either cytological or histological diagnosis (p < 0.05). VEGF mRNA and endostatin mRNA were significantly more likely to be expressed in squamous cell carcinomas (SCC) than in cervical intraepithelial neoplasia (CIN) III (p < 0.01 and p < 0.05), and obviously also more likely to be expressed in CINII than in CINI and in CINIII than in CINII (p < 0.05). Eleven inflammation lesions gave positive results by cytology but negative results by RT-PCR for VEGF and endostatin mRNA. Twenty-four SCC lesions gave false-negative or precancerous lesion results by cytology but positive results by RT-PCR for VEGF and/or endostatin mRNA expression.
  
  
  CONCLUSIONS
  Transcription levels of VEGF and endostatin by RT-PCR may be an adjunct to cytology screening for early detection of cervical carcinomas and may determine the progressive potentiality of individual lesions, especially in high-risk patients.
authors.affiliation: Department of Gynecology, First Affiliated Hospital, China Medical University, Shenyang, PR China.
authors.forename: Ming-Zhe
authors.initials: MZ
authors.lastname: Wu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Wu
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Fu
authors.forename: Guang-Ping
authors.initials: GP
authors.lastname: Wu
authors.forename: Ke-Jun
authors.initials: KJ
authors.lastname: Guo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003581:Cytodiagnosis; D055088:Early Detection of Cancer; D043169:Endostatins / Q000302:isolation & purification*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis*; D014158:Transcription, Genetic; D042461:Vascular Endothelial Growth Factor A / Q000302:isolation & purification*; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis* / Q000473:pathology
chemical_list: D043169:Endostatins; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A