title: Structure-function studies of the Neisseria gonorrhoeae major outer membrane porin.
abstract: The major outer membrane porin (PorB) expressed by Neisseria gonorrhoeae plays multiple roles during infection, in addition to its function as an outer membrane pore. We have generated a panel of mutants of N. gonorrhoeae strain FA1090 expressing a variety of mutant porB genes that all function as porins. We identified multiple regions of porin that are involved in its binding to the complement regulatory factors C4b-binding protein and factor H and confirmed that the ability to bind at least one factor is required for FA1090 to survive the bactericidal effects of human serum. We tested the ability of these mutants to inhibit both apoptosis and the oxidative burst in polymorphonuclear leukocytes but were unable to identify the porin domains required for either function. This study has identified nonessential porin domains and some potentially essential portions of the protein and has further expanded our understanding of the contribution of the porin domains required for complement regulation.
authors.affiliation: Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.
authors.forename: Adrienne
authors.initials: A
authors.lastname: Chen
authors.forename: H Steven
authors.initials: HS
authors.lastname: Seifert
mesh_terms: D000595:Amino Acid Sequence; D017209:Apoptosis; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015935:Complement C4b / Q000378:metabolism*; D017242:Complement Factor H / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D018272:Porins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D016897:Respiratory Burst
chemical_list: D001425:Bacterial Outer Membrane Proteins; D018272:Porins; C057699:porin protein, Neisseria; D015935:Complement C4b; D017242:Complement Factor H
references.citation: Infect Immun. 1999 May;67(5):2406-13
references.pmid: 10225902
references.citation: EMBO J. 2000 Oct 16;19(20):5332-43
references.pmid: 11032801
references.citation: Infect Immun. 2011 Nov;79(11):4447-58
references.pmid: 21844239
references.citation: J Immunol. 2005 Mar 15;174(6):3545-50
references.pmid: 15749891
references.citation: Infect Immun. 2007 Aug;75(8):4071-81
references.pmid: 17526744
references.citation: Methods Enzymol. 1994;236:137-47
references.pmid: 7968606
references.citation: Infect Immun. 2010 Mar;78(3):994-1003
references.pmid: 20028813
references.citation: J Exp Med. 1998 Mar 2;187(5):743-52
references.pmid: 9480984
references.citation: J Bacteriol. 1963 Jun;85:1274-9
references.pmid: 14047217
references.citation: Cell. 1996 May 3;85(3):391-402
references.pmid: 8616894
references.citation: Proc Natl Acad Sci U S A. 1988 Sep;85(18):6841-5
references.pmid: 2457919
references.citation: Cell Microbiol. 2008 Nov;10(11):2257-70
references.pmid: 18684112
references.citation: Infect Immun. 2012 Oct;80(10):3417-28
references.pmid: 22825445
references.citation: Infect Immun. 2004 Nov;72(11):6408-17
references.pmid: 15501771
references.citation: Cell Microbiol. 2006 Nov;8(11):1780-90
references.pmid: 16803582
references.citation: Mol Microbiol. 2001 Jun;40(6):1301-10
references.pmid: 11442829
references.citation: PLoS Pathog. 2009 Oct;5(10):e1000629
references.pmid: 19851451
references.citation: Electrophoresis. 1997 Dec;18(15):2714-23
references.pmid: 9504803
references.citation: J Immunol. 2007 Apr 1;178(7):4489-97
references.pmid: 17372007
references.citation: J Immunol. 2001 Jun 1;166(11):6764-70
references.pmid: 11359834
references.citation: J Immunol. 2002 Feb 15;168(4):1533-7
references.pmid: 11823477
references.citation: J Biol Chem. 1988 Jan 15;263(2):945-51
references.pmid: 2826469
references.citation: Cell Microbiol. 2003 Feb;5(2):99-109
references.pmid: 12580946
references.citation: Nat Protoc. 2010 Apr;5(4):725-38
references.pmid: 20360767
references.citation: J Immunol. 2006 Feb 15;176(4):2373-80
references.pmid: 16455995
references.citation: Infect Immun. 1983 May;40(2):816-9
references.pmid: 6404835
references.citation: Infect Immun. 2006 Jul;74(7):4266-73
references.pmid: 16790801
references.citation: Proc Natl Acad Sci U S A. 1987 Nov;84(22):8135-9
references.pmid: 2825179
references.citation: Proc Natl Acad Sci U S A. 2005 Nov 22;102(47):17142-7
references.pmid: 16275906
references.citation: J Bacteriol. 1999 Sep;181(18):5591-9
references.pmid: 10482498
references.citation: BMC Bioinformatics. 2008 Jan 23;9:40
references.pmid: 18215316
references.citation: Fertil Steril. 1979 Jul;32(1):61-6
references.pmid: 456632
references.citation: J Exp Med. 2001 Feb 5;193(3):281-95
references.pmid: 11157049
references.citation: Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):9070-5
references.pmid: 10922061
references.citation: EMBO J. 1999 Jan 15;18(2):339-52
references.pmid: 9889191
references.citation: Infect Immun. 1988 Apr;56(4):762-5
references.pmid: 3126141
references.citation: J Exp Med. 1998 Aug 17;188(4):671-80
references.pmid: 9705949
references.citation: Infect Immun. 2000 Nov;68(11):6215-22
references.pmid: 11035728
references.citation: J Infect Dis. 1985 Aug;152(2):339-43
references.pmid: 3928769
references.citation: Proc Natl Acad Sci U S A. 2010 Apr 13;107(15):6811-6
references.pmid: 20351243
references.citation: Mol Microbiol. 1999 Feb;31(3):903-13
references.pmid: 10048033
references.citation: EMBO J. 2002 Apr 15;21(8):1916-29
references.pmid: 11953311
references.citation: Infect Immun. 1995 Jan;63(1):160-7
references.pmid: 7806353
references.citation: J Immunol. 2008 Mar 1;180(5):3426-35
references.pmid: 18292569
references.citation: J Exp Med. 1998 Sep 7;188(5):941-52
references.pmid: 9730895
references.citation: FEBS Lett. 1996 Sep 16;393(2-3):297-302
references.pmid: 8814308