title: Alveolar bone regeneration potential of a traditional Chinese medicine, Bu-Shen-Gu-Chi-Wan, in experimental periodontitis.
abstract: OBJECTIVE
  The aim of this study was to investigate the effect of a traditional Chinese medicine, Bu-Shen-Gu-Chi-Wan, on experimental periodontitis and bone regeneration in rat.
  
  
  METHODS
  Sixty female Sprague-Dawley rats were divided equally into three groups: a healthy control group (Group N); a periodontitis group (Group P); and the Bu-Shen-Gu-Chi-Wan treatment group (Group T). A 0.2-mm wire was placed around the maxillary first molar and Porphyromonas gingivalis was injected into the gingival sulcus. Rats in different groups were administered 0.9% normal saline or Bu-Shen-Gu-Chi-Wan solution (4 g/kg of body weight, for three alternate days), and the animals were killed after 4 wk. Morphological analysis of alveolar bone rebuilding was performed using micro-computed tomography (micro-CT) and stereomicroscopy, and the variation of inflammation in the periodontium was determined histologically. The serum levels of the proinflammatory cytokines interleukin-1beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) and of the bone-turnover biomarkers pyridinoline cross-linked carboxyterminal telopeptide of type I collagen (ICTP) and osteocalcin (OC) were determined using radioimmunoassays.
  
  
  RESULTS
  After treatment with Bu-Shen-Gu-Chi-Wan, there were significant decreases in the levels of IL-1β, TNF-α, ICTP and OC and decreased inflammatory infiltration in the periodontal tissues of Group T. significant changes in alveolar bone volume and density were detected by micro-CT, but stereomicroscopy did not detect a significant improvement of alveolar bone height.
  
  
  CONCLUSIONS
  The data of the present study suggest that the traditional Chinese medicine, Bu-Shen-Gu-Chi-Wan, has anti-inflammatory function in experimental periodontitis and may simultaneously improve alveolar bone remodeling.
authors.affiliation: Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Sichuan, China; Department of Periodontology, West China Hospital of Stomatology, Sichuan University, Sichuan, China.
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.forename: Q
authors.initials: Q
authors.lastname: Wen
authors.forename: J
authors.initials: J
authors.lastname: Xue
authors.forename: Y
authors.initials: Y
authors.lastname: Ding
mesh_terms: D016301:Alveolar Bone Loss / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D000539:Alveolar Process / Q000187:drug effects / Q000473:pathology; D000818:Animals; D015519:Bone Density / Q000187:drug effects; D001861:Bone Regeneration / Q000187:drug effects*; D016723:Bone Remodeling / Q000187:drug effects; D024042:Collagen Type I / Q000097:blood; D029768:Cornus; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D053583:Interleukin-1beta / Q000097:blood; D008963:Molar / Q000473:pathology; D015675:Osteocalcin / Q000097:blood; D010455:Peptides / Q000097:blood; D010518:Periodontitis / Q000188:drug therapy* / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000502:physiology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D032267:Rehmannia; D019237:Tooth Cervix / Q000473:pathology; D064028:Tracheophyta; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D024042:Collagen Type I; D004365:Drugs, Chinese Herbal; D053583:Interleukin-1beta; D010455:Peptides; D014409:Tumor Necrosis Factor-alpha; C052929:collagen type I trimeric cross-linked peptide; D015675:Osteocalcin
keywords: anti-inflammation; bone regeneration; experimental periodontitis; micro-CT; traditional Chinese medicine