title: Functional expression of Schizosaccharomyces pombe Vba2p in the vacuolar membrane of Saccharomyces cerevisiae.
abstract: A vacuolar membrane protein, Vba2p of Schizosaccharomyces pombe, is involved in basic amino acid uptake by intact cells. Here we found evidence that Vba2p mediated ATP-dependent lysine uptake by vacuolar membrane vesicles of Saccharomyces cerevisiae. Vba2p was also responsible for quinidine sensitivity, and the addition of lysine improved cell growth on quinidine-containing media. These findings should be useful for further characterization of Vba2p.
authors.affiliation: Department of Applied Bioresource Science, Faculty of Agriculture, Ehime University.
authors.forename: Pongsanat
authors.initials: P
authors.lastname: Pongcharoen
authors.forename: Miyuki
authors.initials: M
authors.lastname: Kawano-Kawada
authors.forename: Tomoko
authors.initials: T
authors.lastname: Iwaki
authors.forename: Naoko
authors.initials: N
authors.lastname: Sugimoto
authors.forename: Takayuki
authors.initials: T
authors.lastname: Sekito
authors.forename: Koichi
authors.initials: K
authors.lastname: Akiyama
authors.forename: Kaoru
authors.initials: K
authors.lastname: Takegawa
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Kakinuma
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D026923:Amino Acid Transport Systems, Basic / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D007425:Intracellular Membranes / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism*; D014617:Vacuoles / Q000235:genetics*
chemical_list: D026923:Amino Acid Transport Systems, Basic; D029702:Schizosaccharomyces pombe Proteins; D000255:Adenosine Triphosphate; D008239:Lysine