title: Growth Inhibition and Morphological Alterations of Trichophyton Rubrum Induced by Essential oil from Cymbopogon Winterianus Jowitt Ex Bor.
abstract: Trichophyton rubrum is one of the most common fungi causer of dermatophytosis, mycosis that affect humans and animals around the world. Researches aiming new products with antifungal activity become necessary to overcome difficulties on treatment of these infections. Accordingly, this study aimed to investigate the antifungal activity of essential oil from Cymbopogon winterianus against the dermatophyte T. rubrum. The antifungal screening was performed by solid medium diffusion method with 16 T. rubrum strains, minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC) were determined using the microdilution method. The effects on mycelial dry weight and morphology were also observed. Screening showed essential oil in natura inhibited all the tested strains, with inhibition zones between 24-28 mm diameter. MIC50 and MIC90 values of the essential oil were 312 μg/mL for nearly all the essayed strains (93.75 %) while the MFC50 and MFC90 values were about eight times higher than MIC for all tested strains. All tested essential oil concentrations managed to inhibit strongly the mycelium development. Main morphological changes on the fungal strains observed under light microscopy, which were provided by the essential oil include loss of conidiation, alterations concerning form and pigmentation of hyphae. In the oil presence, colonies showed folds, cream color and slightly darker than the control, pigment production was absent on the reverse and with evident folds. It is concluded that C. winterianus essential oil showed activity against T. rubrum. Therefore, it could be known as potential antifungal compound especially for protection against dermatophytosis.
authors.affiliation: Laboratório de Micologia, Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal da Paraíba , João Pessoa, PB , Brasil.
authors.forename: Fillipe
authors.initials: F
authors.lastname: de Oliveira Pereira
authors.forename: Paulo
authors.initials: P
authors.lastname: Alves Wanderley
authors.forename: Fernando Antônio
authors.initials: FA
authors.lastname: Cavalcanti Viana
authors.forename: Rita
authors.initials: R
authors.lastname: Baltazar de Lima
authors.forename: Frederico
authors.initials: F
authors.lastname: Barbosa de Sousa
authors.forename: Edeltrudes
authors.initials: E
authors.lastname: de Oliveira Lima
keywords: Cymbopogon; Trichophyton; essential oil
references.citation: J Am Acad Dermatol. 1998 Aug;39(2 Pt 2):379-80
references.pmid: 9703159
references.citation: Mycoses. 2003 Sep;46(8):322-8
references.pmid: 12950902
references.citation: J Clin Microbiol. 2000 Sep;38(9):3329-36
references.pmid: 10970379
references.citation: Microbios. 1996;86(349):237-46
references.pmid: 8893526
references.citation: Rev Soc Bras Med Trop. 2002 Jan-Feb;35(1):19-22
references.pmid: 11873256
references.citation: Braz J Microbiol. 2010 Jan;41(1):28-33
references.pmid: 24031459
references.citation: Diagn Microbiol Infect Dis. 1992 Jan;15(1):21-34
references.pmid: 1309690
references.citation: J Ethnopharmacol. 2005 Aug 22;100(1-2):80-4
references.pmid: 15964727
references.citation: Mycoses. 1993 Sep-Oct;36(9-10):333-6
references.pmid: 8015567
references.citation: Can J Microbiol. 2001 Jan;47(1):9-17
references.pmid: 15049444
references.citation: J Toxicol Clin Exp. 1990 Oct;10(6):361-73
references.pmid: 2130180
references.citation: Trends Microbiol. 2003 Jun;11(6):272-9
references.pmid: 12823944
references.citation: Braz J Microbiol. 2008 Jan;39(1):91-7
references.pmid: 24031186
references.citation: Int J Food Microbiol. 2004 Aug 1;94(3):223-53
references.pmid: 15246235
references.citation: Rev Iberoam Micol. 1999 Mar;16(1):16-22
references.pmid: 18473586
references.citation: J Invest Dermatol. 1987 Nov;89(5):529-34
references.pmid: 3668298
references.citation: J Clin Microbiol. 2005 Apr;43(4):1917-20
references.pmid: 15815018
references.citation: J Antibiot (Tokyo). 1995 Apr;48(4):306-10
references.pmid: 7775267
references.citation: Mycol Res. 2006 Nov;110(Pt 11):1355-60
references.pmid: 17070026
references.citation: J Ethnopharmacol. 2005 Feb 28;97(2):305-11
references.pmid: 15707770
references.citation: J Appl Microbiol. 2000 Jan;88(1):170-5
references.pmid: 10735256
references.citation: Int J Dermatol. 2006 Jul;45(7):822-7
references.pmid: 16863519
references.citation: Microbiol Res. 2008;163(3):337-44
references.pmid: 16870411
references.citation: Clin Microbiol Rev. 1995 Apr;8(2):240-59
references.pmid: 7621400
references.citation: Microbiol Rev. 1995 Jun;59(2):201-22
references.pmid: 7603409
references.citation: Rev Iberoam Micol. 2002 Mar;19(1):36-39
references.pmid: 12716229
references.citation: J Clin Microbiol. 2006 Jan;44(1):98-101
references.pmid: 16390955
references.citation: Food Chem Toxicol. 2008 Feb;46(2):446-75
references.pmid: 17996351