title: Investigation of the competition between cell/surface and cell/cell interactions during neuronal cell culture on a micro-engineered surface.
abstract: To investigate the respective roles of topography and cell/cell interactions in the development of a guided neuronal network on an engineered surface, micropatterned PDMS substrates were generated with different microgrooves geometry and investigated for the influence of cell density on neurite outgrowth and alignment. Through this systematic investigation, using a human neuronal stem cell line, the rules of neuronal network development and guiding could be learned. The results show that when cell density is increased the influence on neuritic outgrowth and alignment is very different for the various grooves geometries. The data emphasized the competition, in neurite development, between physical cues brought by surface topographical features and cell to cell communications. These results can be of particular interest for designing functional neuronal networks with a controlled architecture.
authors.affiliation: CNRS-LAAS, 7 avenue du colonel Roche, F-31400, Toulouse, France; Université de Toulouse, UPS, LAAS, F-31400, Toulouse, France; ITAV, Centre Pierre Potier, F-31106, Toulouse, France.
authors.forename: Amélie
authors.initials: A
authors.lastname: Béduer
authors.forename: Inès
authors.initials: I
authors.lastname: Gonzales-Calvo
authors.forename: Christophe
authors.initials: C
authors.lastname: Vieu
authors.forename: Isabelle
authors.initials: I
authors.lastname: Loubinoux
authors.forename: Laurence
authors.initials: L
authors.lastname: Vaysse
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects; D002452:Cell Count; D002454:Cell Differentiation; D002460:Cell Line; D004129:Dimethylpolysiloxanes / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D009415:Nerve Net / Q000033:anatomy & histology* / Q000187:drug effects / Q000502:physiology; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D016501:Neurites / Q000187:drug effects / Q000648:ultrastructure*; D009757:Nylons / Q000737:chemistry / Q000494:pharmacology; D013499:Surface Properties; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D004129:Dimethylpolysiloxanes; D009757:Nylons; C085707:poly(dimethylsiloxane)-polyamide copolymer