title: A systematic investigation on biological activities of a novel double zwitterionic Schiff base Cu(II) complex.
abstract: Double zwitterionic amino acid Schiff base, o-vanillylidene-L-histidine (OVHIS) and its copper complex (CuOVHIS) have been synthesized and characterized. CuOVHIS has distorted octahedral geometry, and OVHIS coordinates the copper ion in a tetradentate manner (N2O2). The pKa of OVHIS in aqueous solution was studied by potentiometric and spectrophotometric methods. DNA binding behavior of the compounds was investigated using spectrophotometric, cyclic voltammetric, and viscosity methods. The efficacy of DNA cleaving nature was tested on pUC19 DNA. The in vitro biological activity was tested against various micro organisms. The effect of CuOVHIS on the surface feature of Escherichia coli was analyzed by SEM. DPPH assay studies revealed that CuOVHIS has higher antioxidant activity. OVHIS inhibits proliferation of HCT117 cells with half maximal inhibition (IC50) of 71.15±0.67. Chelation of OVHIS with Cu(II) ion enhances the inhibition of proliferation action (IC50=53.14±0.67).
authors.affiliation: Chemistry Research Centre, National Engineering College, K.R. Nagar, Kovilpatti 628503, Thoothukudi District, Tamil Nadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Thalamuthu
authors.forename: B
authors.initials: B
authors.lastname: Annaraj
authors.forename: M A
authors.initials: MA
authors.lastname: Neelakantan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000648:ultrastructure; D002417:Cattle; D045744:Cell Line, Tumor; D056831:Coordination Complexes / Q000494:pharmacology*; D003300:Copper / Q000494:pharmacology*; D004247:DNA; D053837:DNA Cleavage; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D055664:Electrochemical Techniques; D004578:Electron Spin Resonance Spectroscopy; D004583:Electrons; D005658:Fungi / Q000187:drug effects; D006639:Histidine / Q000737:chemistry; D006801:Humans; D007477:Ions; D007700:Kinetics; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D012545:Schiff Bases / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared; D017550:Spectroscopy, Fourier Transform Infrared; D044366:Transition Temperature; D014783:Viscosity
chemical_list: D000900:Anti-Bacterial Agents; D056831:Coordination Complexes; D007477:Ions; D012545:Schiff Bases; D006639:Histidine; D003300:Copper; D004247:DNA; C427203:calf thymus DNA; D004121:Dimethyl Sulfoxide
keywords: Acid–base equilibria; Biological activity; Copper complex; Double zwitterionic Schiff base; Spectral studies