title: Overexpression of integrin-linked kinase (ILK) promotes glioma cell invasion and migration and down-regulates E-cadherin via the NF-κB pathway.
abstract: Integrin-linked kinase (ILK) is a ubiquitously expressed serine/threonine protein kinase that has been implicated in cancer development, progression and metastasis. The aim of the present study was to characterize the role of ILK in glioma cell invasion and migration. We generated a recombinant eukaryotic expression vector containing the human ILK gene and transfected it into human glioma SHG-44 cells. Real-time PCR and western blot analysis were used to identify the stable transformants. The wound healing and Transwell invasion assays showed that ectopic overexpression of ILK in SHG-44 cells significantly promoted their migration and invasion capabilities in culture. This was accompanied by a decrease in expression of E-cadherin and an increase in expression of Snail and Slug. Moreover, the decrease in E-cadherin expression induced by ILK overexpression was greatly restored by the nuclear factor-κB (NF-κB) inhibitor BAY 11-7028 or small interfering RNA targeting NF-κB p65, indicating an involvement of NF-κB in ILK-induced down-regulation of E-cadherin. In conclusion, our data underscore a novel role for ILK in glioma invasion and metastasis processes, implicating potential for therapeutic interference.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Shenyang, 110001, Liaoning, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liang
authors.forename: Shuqin
authors.initials: S
authors.lastname: Zhang
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Jianwu
authors.initials: J
authors.lastname: Qiu
authors.forename: Yunjie
authors.initials: Y
authors.lastname: Wang
mesh_terms: D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma; D006801:Humans; D009361:Neoplasm Invasiveness; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D051996:Transcription Factor RelA / Q000378:metabolism*; D000099200:Scaffold Protein ILK
chemical_list: D015703:Antigens, CD; D015820:Cadherins; D017346:Protein Serine-Threonine Kinases; D051996:Transcription Factor RelA; D000099200:Scaffold Protein ILK; C513384:CDH1 protein, human; C498143:RELA protein, human
references.citation: Cell Signal. 2012 Jun;24(6):1323-32
references.pmid: 22570869
references.citation: Int J Cancer. 2010 Mar 15;126(6):1436-44
references.pmid: 19676046
references.citation: Clin Cancer Res. 2003 Oct 1;9(12):4409-14
references.pmid: 14555513
references.citation: J Recept Signal Transduct Res. 2012 Apr;32(2):120-7
references.pmid: 22384810
references.citation: Virchows Arch. 2011 Jan;458(1):99-107
references.pmid: 21136077
references.citation: Dev Dyn. 2008 Oct;237(10):2737-47
references.pmid: 18773488
references.citation: J Mol Histol. 2013 Apr;44(2):183-9
references.pmid: 23108908
references.citation: J Clin Oncol. 2003 Apr 15;21(8):1624-36
references.pmid: 12697889
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: Int J Biochem Cell Biol. 2011 Sep;43(9):1294-304
references.pmid: 21601006
references.citation: CA Cancer J Clin. 2010 May-Jun;60(3):166-93
references.pmid: 20445000
references.citation: N Engl J Med. 2001 Jan 11;344(2):114-23
references.pmid: 11150363
references.citation: Oncogene. 2006 Jun 1;25(23):3237-46
references.pmid: 16407822
references.citation: Mol Cancer Ther. 2007 Jun;6(6):1692-700
references.pmid: 17575101
references.citation: Acta Histochem. 2013 Mar;115(2):128-36
references.pmid: 22683084
references.citation: Tumour Biol. 2012 Oct;33(5):1477-84
references.pmid: 22544614
references.citation: Br J Surg. 2000 Aug;87(8):992-1005
references.pmid: 10931041
references.citation: J Cell Sci. 2008 Oct 1;121(Pt 19):3121-32
references.pmid: 18799788
references.citation: Tumour Biol. 2013 Feb;34(1):359-68
references.pmid: 23065574
references.citation: Lancet Oncol. 2009 May;10(5):459-66
references.pmid: 19269895
references.citation: PLoS One. 2011 Feb 09;6(2):e16984
references.pmid: 21347395
references.citation: Biochim Biophys Acta. 2004 Apr 1;1691(1):1-15
references.pmid: 15053919
references.citation: Nat Rev Cancer. 2005 Jan;5(1):51-63
references.pmid: 15630415
references.citation: Proc Natl Acad Sci U S A. 1998 Sep 15;95(19):11211-6
references.pmid: 9736715
references.citation: Exp Cell Res. 2013 Aug 1;319(13):2058-2072
references.pmid: 23707970
references.citation: J Cell Biochem. 2008 Jun 1;104(3):733-44
references.pmid: 18253935
references.citation: Oncogene. 2011 Jun 16;30(24):2778-88
references.pmid: 21278793
references.citation: Nature. 1996 Jan 4;379(6560):91-6
references.pmid: 8538749
references.citation: Cell Mol Life Sci. 2008 Nov;65(23):3756-88
references.pmid: 18726070
references.citation: Int J Med Sci. 2013 Jun 14;10(8):995-1002
references.pmid: 23801886
references.citation: Int J Biochem Mol Biol. 2012;3(2):117-36
references.pmid: 22773954
references.citation: Clin Exp Metastasis. 2012 Dec;29(8):957-69
references.pmid: 22638656
references.citation: Cell Mol Life Sci. 2007 Feb;64(4):458-78
references.pmid: 17260089
references.citation: Oncogene. 2001 Jan 4;20(1):133-40
references.pmid: 11244511
references.citation: World J Gastroenterol. 2011 Aug 14;17(30):3487-96
references.pmid: 21941415
references.citation: Mol Cancer Ther. 2005 Nov;4(11):1681-8
references.pmid: 16275989