title: Safe pseudovirus-based assay for neutralization antibodies against influenza A(H7N9) virus.
abstract: Serologic studies are urgently needed to assist in understanding an outbreak of influenza A(H7N9) virus. However, a biosafety level 3 laboratory is required for conventional serologic assays with live lethal virus. We describe a safe pseudovirus-based neutralization assay with preliminary assessment using subtype H7N9-infected samples and controls.
authors.forename: Chao
authors.initials: C
authors.lastname: Qiu
authors.forename: Yang
authors.initials: Y
authors.lastname: Huang
authors.forename: Anli
authors.initials: A
authors.lastname: Zhang
authors.forename: Di
authors.initials: D
authors.lastname: Tian
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Wan
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Zhang
authors.forename: Wanju
authors.initials: W
authors.lastname: Zhang
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Yuan
authors.forename: Yunwen
authors.initials: Y
authors.lastname: Hu
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhang
authors.forename: Jianqing
authors.initials: J
authors.lastname: Xu
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D057134:Antibodies, Neutralizing / Q000097:blood; D036022:Capsid Proteins / Q000235:genetics; D003001:Cloning, Molecular; D005818:Genetic Engineering; D005822:Genetic Vectors; D057809:HEK293 Cells; D006801:Humans; D064766:Influenza A Virus, H7N9 Subtype / Q000235:genetics / Q000276:immunology*; D007251:Influenza, Human / Q000097:blood / Q000276:immunology* / Q000821:virology; D016086:Lentivirus / Q000235:genetics; D008875:Middle Aged; D009500:Neutralization Tests
chemical_list: D057134:Antibodies, Neutralizing; D036022:Capsid Proteins
keywords: Influenza; neutralization tests; pseudovirus; serology; subtype H7N9; viruses
references.citation: N Engl J Med. 2013 May 16;368(20):1888-97
references.pmid: 23577628
references.citation: Clin Infect Dis. 2012 Jan 1;54(1):17-24
references.pmid: 22052887
references.citation: PLoS One. 2010 Jun 09;5(6):e11036
references.pmid: 20543954
references.citation: Euro Surveill. 2013 Apr 11;18(15):20452
references.pmid: 23594574
references.citation: J Mol Genet Med. 2012;7:315-20
references.pmid: 23577044
references.citation: J Clin Virol. 2007 May;39(1):27-33
references.pmid: 17409017