title: Performance of transport and selective media for swine Bordetella bronchiseptica recovery and it comparison to polymerase chain reaction detection.
abstract: Three comparative assays were performed seeking to improve the sensitivity of the diagnosis of Bordetella bronchiseptica infection analyzing swine nasal swabs. An initial assay compared the recovery of B. bronchiseptica from swabs simultaneously inoculated with B. bronchiseptica and some interfering bacteria, immersed into three transport formulations (Amies with charcoal, trypticase soy broth and phosphate buffer according to Soerensen supplemented with 5% of bovine fetal serum) and submitted to different temperatures (10°C and 27°C) and periods of incubation (24, 72 and 120 hours). A subsequent assay compared three selective media (MacConkey agar, modified selective medium G20G and a ceftiofur medium) for their recovery capabilities from clinical specimens. One last assay compared the polymerase chain reaction to the three selective media. In the first assay, the recovery of B. bronchiseptica from transport systems was better at 27°C and the three formulations had good performances at this temperature, but the collection of qualitative and quantitative analysis indicated the advantage of Amies medium for nasal swabs transportation. The second assay indicated that MacConkey agar and modified G20G had similar results and were superior to the ceftiofur medium. In the final assay, polymerase chain reaction presented superior capability of B. bronchiseptica detection to culture procedures.
authors.affiliation: Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul , Porto Alegre, RS , Brasil ; Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul , Porto Alegre, RS , Brasil.
authors.forename: Tania Alen
authors.initials: TA
authors.lastname: Coutinho
authors.forename: Mari Lourdes
authors.initials: ML
authors.lastname: Bernardi
authors.forename: Marisa Ribeiro
authors.initials: MR
authors.lastname: de Itapema Cardoso
authors.forename: Sandra Maria
authors.initials: SM
authors.lastname: Borowski
authors.forename: Andrea Micke
authors.initials: AM
authors.lastname: Moreno
authors.forename: David Emilio Santos Neves
authors.initials: DE
authors.lastname: de Barcellos
keywords: Bordetella bronchiseptica; PCR; selective media; swine; transport media
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