title: Nanoparticles laden in situ gel of levofloxacin for enhanced ocular retention.
abstract: Availability of proper concentration of medicament on to the corneal surface is a challenging task. Many novel formulations, i.e. hydrogels, nanoparticles, ocuserts, etc. had been tested to improve ocular bioavailability, out of which our group found, in situ gel and polymeric nanoparticle are the most interesting approach to achieve ocular retention. We found that in situ gel stay only for 12 h and poly(lactic-co-glycolic acid (PLGA) nanoparticles are non mucoadhesive in nature so we try to combine both these formulations and termed it as "Nanoparticle laden in situ gel". Here we prepare nanoparticle laden in situ gel containing levofloxacin encapsulated PLGA nanoparticle, incorporated in chitosan in situ gel and evaluated its ocular retention by gamma scintigraphy in rabbits. The observations of acquired gamma camera images showed good retention over the entire precorneal area. From static and dynamic gamma scintigraphy evaluation, we can be interpret that developed nanoparticle laden in situ gel formulation cleared at a very slow rate and remained at corneal surface for longer duration than marketed formulation, in situ gel and nanosuspension alone.
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard , New Delhi , India and.
authors.forename: Himanshu
authors.initials: H
authors.lastname: Gupta
authors.forename: M
authors.initials: M
authors.lastname: Aqil
authors.forename: R K
authors.initials: RK
authors.lastname: Khar
authors.forename: Asgar
authors.initials: A
authors.lastname: Ali
authors.forename: Aseem
authors.initials: A
authors.lastname: Bhatnagar
authors.forename: Gaurav
authors.initials: G
authors.lastname: Mittal
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D001682:Biological Availability; D048271:Chitosan / Q000737:chemistry; D003315:Cornea / Q000378:metabolism*; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry; D005782:Gels; D019344:Lactic Acid / Q000737:chemistry; D064704:Levofloxacin / Q000008:administration & dosage* / Q000493:pharmacokinetics; D008297:Male; D053758:Nanoparticles; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D011877:Radionuclide Imaging / Q000379:methods; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D000900:Anti-Bacterial Agents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005782:Gels; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D064704:Levofloxacin; D048271:Chitosan