title: Phospholipase gene expression during Paracoccidioides brasiliensis morphological transition and infection.
abstract: Phospholipase is an important virulence factor for pathogenic fungi. In this study, we demonstrate the following: (i) the Paracoccidioides brasiliensis pld gene is preferentially expressed in mycelium cells, (ii) the plb1 gene is mostly up-regulated by infection after 6 h of co-infection of MH-S cells or during BALB/c mice lung infection, (iii) during lung infection, plb1, plc and pld gene expression are significantly increased 6-48 h post-infection compared to 56 days after infection, strongly suggesting that phospholipases play a role in the early events of infection, but not during the chronic stages of pulmonary infection by P. brasiliensis.
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Goiás, GoiâniaGO, Brasil.
authors.forename: Deyze Alencar
authors.initials: DA
authors.lastname: Soares
authors.forename: Marilia Barros
authors.initials: MB
authors.lastname: Oliveira
authors.forename: Adriane Feijó
authors.initials: AF
authors.lastname: Evangelista
authors.forename: Emerson José
authors.initials: EJ
authors.lastname: Venancio
authors.forename: Rosângela Vieira
authors.initials: RV
authors.lastname: Andrade
authors.forename: Maria Sueli Soares
authors.initials: MS
authors.lastname: Felipe
authors.forename: Silvana
authors.initials: S
authors.lastname: Petrofeza
mesh_terms: D000818:Animals; D015870:Gene Expression; D016676:Macrophages, Alveolar / Q000382:microbiology*; D008297:Male; D008807:Mice, Inbred BALB C; D010228:Paracoccidioides / Q000166:cytology / Q000201:enzymology / Q000472:pathogenicity; D010229:Paracoccidioidomycosis; D010740:Phospholipases / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D037521:Virulence Factors; D010740:Phospholipases
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