title: Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli.
abstract: With the advent of recombinant DNA technology, recombinant protein expression has become an important tool in the study of the structure, function and identification of new proteins, especially those with therapeutic functions. Escherichia coli has been the predominant prokaryote used in genetic engineering studies due to the abundance of information about its metabolism. Despite significant advances in molecular biology and immunology of infections, there are as yet no prophylactic drugs capable of preventing visceral leishmaniasis. It is therefore important to identify specific antigens in order to develop vaccines and diagnostic kits against this disease. The objective of this study was to evaluate the influence of culture medium on the production of eIF antigen from Leishmania chagasi in recombinant Escherichia coli. An induction procedure using IPTG was carried out in a series of trials, to observe the influence of culture medium (2xTY, TB) under expression of the recombinant eIF protein. Results showed that recombinant protein expression was associated to growth and that the highest eIF antigen expression was obtained in the 2xTY medium.
authors.affiliation: Laboratório de Engenharia Bioquímica, Departamento de Engenharia Química, Universidade Federal do Rio Grande do Norte , Natal, RN , Brasil.
authors.forename: Michelle Rossana Ferreira
authors.initials: MR
authors.lastname: Vaz
authors.forename: Ricardo Luiz Soares
authors.initials: RL
authors.lastname: de França
authors.forename: Sirtys Santos Lessa
authors.initials: SS
authors.lastname: de Andrade
authors.forename: Francisco Canindé
authors.initials: FC
authors.lastname: de Sousa Junior
authors.forename: Everaldo Silvino
authors.initials: ES
authors.lastname: Dos Santos
authors.forename: Daniella Regina Arantes
authors.initials: DR
authors.lastname: Martins
authors.forename: Gorete Ribeiro
authors.initials: GR
authors.lastname: de Macedo
keywords: Cultivation; Escherichia coli; Recombinant protein; Visceral leishmaniasis
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