title: Characterization of a novel G3P[3] rotavirus isolated from a lesser horseshoe bat: a distant relative of feline/canine rotaviruses.
abstract: Bats are considered important animal reservoirs for many viruses pathogenic to humans. An approach based on viral metagenomics was used to study gut specimens from 78 insectivorous bats in Yunnan Province, China. Seventy-four reads were found to be related to group A rotavirus (RVA). Further reverse transcription-PCR screening and viral isolation on cell cultures confirmed the presence of a novel RVA strain, named RVA/Bat-tc/MSLH14/2012/G3P[3], in 1 (6%) of 16 lesser horseshoe bats. Full genomic sequencing analyses showed that MSLH14 possessed the genotype constellation G3-P[3]-I8-R3-C3-M3-A9-N3-T3-E3-H6, which is akin to human and animal rotaviruses believed to be of feline/canine origin. Phylogenetic analysis indicated that VP7 was most closely related to bovine RVA strains from India, whereas VP4 was most closely related to an unusual human RVA strain, CMH222, with animal characteristics isolated in Thailand. The remaining gene segments were only distantly related to a range of animal RVA strains, most of which are believed to be related to feline/canine RVAs. Experimental infection showed that bat RVA strain MSLH14 was highly pathogenic to suckling mice, causing 100% mortality when they were inoculated orally with a titer as low as 5 × 10² 50% tissue culture infective doses. As this virus is not closely related to any known RVA strain, it is tempting to speculate that it is a true bat RVA strain rather than a virus transmitted between species. However, further screening of bat populations, preferably juvenile animals, will be crucial in determining whether or not this virus is widely distributed in the bat population.
authors.affiliation: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China.
authors.forename: Biao
authors.initials: B
authors.lastname: He
authors.forename: Fanli
authors.initials: F
authors.lastname: Yang
authors.forename: Weihong
authors.initials: W
authors.lastname: Yang
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yun
authors.initials: Y
authors.lastname: Feng
authors.forename: Jihua
authors.initials: J
authors.lastname: Zhou
authors.forename: Jinxin
authors.initials: J
authors.lastname: Xie
authors.forename: Ye
authors.initials: Y
authors.lastname: Feng
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Bao
authors.forename: Huancheng
authors.initials: H
authors.lastname: Guo
authors.forename: Yingying
authors.initials: Y
authors.lastname: Li
authors.forename: Lele
authors.initials: L
authors.lastname: Xia
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Jelle
authors.initials: J
authors.lastname: Matthijnssens
authors.forename: Hailin
authors.initials: H
authors.lastname: Zhang
authors.forename: Changchun
authors.initials: C
authors.lastname: Tu
mesh_terms: D000818:Animals; D001499:Bayes Theorem; D005075:Biological Evolution; D002415:Cats; D002417:Cattle; D002681:China / Q000453:epidemiology; D002685:Chiroptera; D005260:Female; D016679:Genome, Viral; D006801:Humans; D051379:Mice; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000235:genetics / Q000821:virology*; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins
references.citation: J Clin Microbiol. 1999 Aug;37(8):2734-6
references.pmid: 10405435
references.citation: Emerg Infect Dis. 2010 May;16(5):859-62
references.pmid: 20409385
references.citation: J Virol. 2010 Jul;84(14):6955-65
references.pmid: 20463061
references.citation: Infect Genet Evol. 2013 Jun;16:103-12
references.pmid: 23403096
references.citation: J Virol. 1991 May;65(5):2562-71
references.pmid: 1850029
references.citation: BMC Evol Biol. 2007 Nov 08;7:214
references.pmid: 17996036
references.citation: J Infect Dis. 2005 Sep 1;192 Suppl 1:S146-59
references.pmid: 16088798
references.citation: Vet Microbiol. 2013 Jan 25;161(3-4):239-46
references.pmid: 22959604
references.citation: J Virol. 2010 Feb;84(4):2013-26
references.pmid: 19939934
references.citation: J Virol. 1988 May;62(5):1625-33
references.pmid: 2833612
references.citation: J Gen Virol. 2011 May;92(Pt 5):1214-1221
references.pmid: 21228122
references.citation: J Virol. 2009 Apr;83(7):2917-29
references.pmid: 19153225
references.citation: J Clin Microbiol. 1990 Jun;28(6):1198-203
references.pmid: 2166076
references.citation: BMC Microbiol. 2009 Nov 23;9:238
references.pmid: 19930627
references.citation: J Virol. 2012 Apr;86(8):4620-30
references.pmid: 22345464
references.citation: J Virol. 2010 Dec;84(24):13004-18
references.pmid: 20926577
references.citation: PLoS One. 2012;7(8):e43986
references.pmid: 22937142
references.citation: Mol Biol Evol. 2010 Oct;27(10):2431-6
references.pmid: 20522727
references.citation: Emerg Infect Dis. 2010 Dec;16(12):1844-52
references.pmid: 21122212
references.citation: J Virol. 2008 Apr;82(7):3204-19
references.pmid: 18216098
references.citation: Proc Biol Sci. 2013 Feb 01;280(1756):20122753
references.pmid: 23378666
references.citation: Vet Microbiol. 2010 Jan 27;140(3-4):246-55
references.pmid: 19781872
references.citation: Curr Opin Virol. 2012 Aug;2(4):426-33
references.pmid: 22683209
references.citation: J Clin Microbiol. 1982 Sep;16(3):473-7
references.pmid: 6182158
references.citation: Acta Microbiol Immunol Hung. 2012 Sep;59(3):411-21
references.pmid: 22982644
references.citation: J Infect Dis. 2009 Nov 1;200 Suppl 1:S167-73
references.pmid: 19817597
references.citation: Virology. 2008 Oct 25;380(2):344-53
references.pmid: 18789808
references.citation: J Virol. 2012 Oct;86(20):10999-1012
references.pmid: 22855479
references.citation: J Med Virol. 2006 Jul;78(7):986-94
references.pmid: 16721863
references.citation: Arch Virol. 2011 Aug;156(8):1397-413
references.pmid: 21597953
references.citation: J Gen Virol. 2013 Jan;94(Pt 1):136-142
references.pmid: 23052396
references.citation: Clin Microbiol Rev. 2006 Jul;19(3):531-45
references.pmid: 16847084
references.citation: J Virol. 2007 Mar;81(5):2382-90
references.pmid: 17166908
references.citation: PLoS One. 2013 Apr 22;8(4):e61950
references.pmid: 23630620
references.citation: J Clin Microbiol. 1997 May;35(5):1272-5
references.pmid: 9114424
references.citation: Exp Mol Pathol. 1989 Oct;51(2):186-204
references.pmid: 2553474
references.citation: Mol Biol Evol. 2011 Oct;28(10):2731-9
references.pmid: 21546353
references.citation: J Med Virol. 2009 Jan;81(1):176-82
references.pmid: 19031442
references.citation: Infect Genet Evol. 2012 Oct;12(7):1427-35
references.pmid: 22613801
references.citation: Proc Soc Exp Biol Med. 1987 Jan;184(1):127-32
references.pmid: 3025888
references.citation: Infect Genet Evol. 2011 Aug;11(6):1396-406
references.pmid: 21609783
references.citation: Emerg Infect Dis. 2013 Apr;19(4):638-40
references.pmid: 23631923
references.citation: Arch Virol. 2007;152(10):1935-40
references.pmid: 17577612
references.citation: J Med Virol. 2011 Jul;83(7):1288-99
references.pmid: 21567432
references.citation: J Gen Virol. 2012 Apr;93(Pt 4):866-875
references.pmid: 22190012
references.citation: J Med Virol. 2007 Jun;79(6):843-51
references.pmid: 17457907
references.citation: Lancet Infect Dis. 2012 Feb;12(2):136-41
references.pmid: 22030330
references.citation: J Virol. 1990 Oct;64(10):5070-5
references.pmid: 2168987
references.citation: Arch Virol. 1994;135(3-4):427-32
references.pmid: 7979978
references.citation: J Virol. 1984 Jul;51(1):233-6
references.pmid: 6328042
references.citation: Arch Virol. 2008;153(8):1621-9
references.pmid: 18604469