title: Differentiation of embryonic stem cells to cardiomyocytes on electrospun nanofibrous substrates.
abstract: The potential of pluripotent embryonic stem cells (ESCs) isolated from the inner mass of blastocysts are investigated for its ability to differentiate on biocompatible electrospun nanofibers, for regeneration of the myocardially infracted heart. Nanostructured poly(d,l-lactide-co-glycolide)/collagen (PLGA/Col) scaffolds with fiber diameters in the range of 300 ± 65 nm, was fabricated by electrospinning to mimic the extracellular matrix of the native tissue. During the culture of embryoid bodies outgrowth on the scaffolds, and further differentiation of ESCs to cardiomyocytes, the PLGA/Col nanofibers was found better than that of the electrospun PLGA nanofibers, where a better interaction and growth of ESC differentiated cardiomyocytes was observed on the composite scaffolds. The phenotypical characteristics of ESC-derived cardiomyocytes and molecular protein expression were carried out by scanning electron microscopy and immunocytochemistry, respectively. Our studies highlight the significance of a suitable material, its architecture, and cell-biomaterial interactions that is essential at a nanoscale level signifying the application of a bioengineered cardiac graft for stem cell differentiation and transplantation, which could be an intriguing strategy for cardiac regeneration.
authors.affiliation: Center for Nanofibers and Nanotechnology, E3-05-14, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, Singapore, 117576.
authors.forename: Molamma P
authors.initials: MP
authors.lastname: Prabhakaran
authors.forename: Laleh Ghasemi
authors.initials: LG
authors.lastname: Mobarakeh
authors.forename: Dan
authors.initials: D
authors.lastname: Kai
authors.forename: Khadijeh
authors.initials: K
authors.lastname: Karbalaie
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Nasr-Esfahani
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D003094:Collagen; D053595:Embryonic Stem Cells / Q000187:drug effects* / Q000648:ultrastructure; D007150:Immunohistochemistry; D057026:Induced Pluripotent Stem Cells / Q000187:drug effects* / Q000648:ultrastructure; D019344:Lactic Acid; D055595:Mechanical Phenomena; D051379:Mice; D008810:Mice, Inbred C57BL; D008855:Microscopy, Electron, Scanning; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000648:ultrastructure; D057139:Nanofibers; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003094:Collagen
keywords: cardiac regeneration; collagen; electrospinning; embryoid bodies; embryonic stem cells