title: Comparison of 4D-microSPECT and microCT for murine cardiac function.
abstract: OBJECTIVE
  The objective of this study was to compare a new generation of four-dimensional micro-single photon emission computed tomography (microSPECT) with microCT for the quantitative in vivo assessment of murine cardiac function.
  
  
  METHODS
  Four-dimensional isotropic cardiac images were acquired from anesthetized normal C57BL/6 mice with either microSPECT (n = 6) or microCT (n = 6). One additional mouse with myocardial infarction (MI) was scanned with both modalities. Prior to imaging, mice were injected with either technetium tetrofosmin for microSPECT or a liposomal blood pool contrast agent for microCT. Segmentation of the left ventricle (LV) was performed using Vitrea (Vital Images) software, to derive global and regional function.
  
  
  RESULTS
  Measures of global LV function between microSPECT and microCT groups were comparable (e.g., ejection fraction = 71 ± 6 % microSPECT and 68 ± 4 % microCT). Regional functional indices (wall motion, wall thickening, regional ejection fraction) were also similar for the two modalities. In the mouse with MI, microSPECT identified a large perfusion defect that was not evident with microCT.
  
  
  CONCLUSIONS
  Despite lower spatial resolution, microSPECT was comparable to microCT in the quantitative evaluation of cardiac function. MicroSPECT offers an advantage over microCT in the ability to evaluate simultaneously myocardial radiotracer distribution and function, simultaneously. MicroSPECT should be considered as an alternative to microCT and magnetic resonance for preclinical cardiac imaging in the mouse.
authors.affiliation: Center for In Vivo Microscopy, Department of Radiology, Duke University Medical Center, Box 3302, Durham, NC, 27710, USA.
authors.forename: Nicholas T
authors.initials: NT
authors.lastname: Befera
authors.forename: Cristian T
authors.initials: CT
authors.lastname: Badea
authors.forename: G Allan
authors.initials: GA
authors.lastname: Johnson
mesh_terms: D000818:Animals; D006321:Heart / Q000000981:diagnostic imaging* / Q000502:physiology; D006352:Heart Ventricles / Q000000981:diagnostic imaging; D051379:Mice; D008810:Mice, Inbred C57BL; D009929:Organ Size; D015899:Tomography, Emission-Computed, Single-Photon; D016276:Ventricular Function; D055114:X-Ray Microtomography
references.citation: Phys Med Biol. 2010 Apr 7;55(7):2023-34
references.pmid: 20299722
references.citation: Med Phys. 2012 Aug;39(8):4943-58
references.pmid: 22894420
references.citation: Comput Med Imaging Graph. 2008 Apr;32(3):239-50
references.pmid: 18243656
references.citation: Circulation. 2004 Sep 21;110(12):1632-7
references.pmid: 15364813
references.citation: Invest Radiol. 2007 Oct;42(10):704-14
references.pmid: 17984768
references.citation: Physiol Rev. 2008 Apr;88(2):389-419
references.pmid: 18391168
references.citation: Invest Radiol. 2007 Feb;42(2):85-94
references.pmid: 17220726
references.citation: Eur J Nucl Med Mol Imaging. 2013 May;40(5):744-58
references.pmid: 23344137
references.citation: Radiat Res. 2008 Aug;170(2):260-3
references.pmid: 18666818
references.citation: Mol Imaging. 2005 Apr-Jun;4(2):110-6
references.pmid: 16105509
references.citation: Med Phys. 2004 Dec;31(12):3324-9
references.pmid: 15651615
references.citation: Radiology. 2007 Nov;245(2):440-8
references.pmid: 17940303
references.citation: Dis Model Mech. 2010 Mar-Apr;3(3-4):138-43
references.pmid: 20212081
references.citation: Appl Opt. 2004 Feb 10;43(5):1053-62
references.pmid: 15008484
references.citation: Am J Physiol Heart Circ Physiol. 2000 Feb;278(2):H652-7
references.pmid: 10666098
references.citation: J Magn Reson Imaging. 2003 Dec;18(6):691-701
references.pmid: 14635154
references.citation: J Cardiovasc Magn Reson. 2011 Sep 05;13:44
references.pmid: 21892953
references.citation: Am J Physiol Heart Circ Physiol. 2011 Jun;300(6):H1983-9
references.pmid: 21490325
references.citation: Magn Reson Med. 2008 Jul;60(1):111-8
references.pmid: 18581419
references.citation: Med Phys. 2006 Jan;33(1):216-24
references.pmid: 16485428
references.citation: Toxicol Pathol. 2006;34(1):111-7
references.pmid: 16507552
references.citation: Med Phys. 2004 Sep;31(9):2680-6
references.pmid: 15487751
references.citation: J Biomed Biotechnol. 2011;2011:497841
references.pmid: 21403831
references.citation: J Nucl Med. 2005 Jun;46(6):1023-7
references.pmid: 15937315
references.citation: Eur J Nucl Med Mol Imaging. 2010 Aug;37(9):1766-77
references.pmid: 20069298
references.citation: J Nucl Med. 2005 Jun;46(6):1005-11
references.pmid: 15937312
references.citation: AJR Am J Roentgenol. 2006 Feb;186(2):300-7
references.pmid: 16423931
references.citation: J Nucl Cardiol. 2007 May-Jun;14(3):371-9
references.pmid: 17556172
references.citation: Acad Radiol. 2007 Jul;14(7):788-94
references.pmid: 17574129
references.citation: Circulation. 2002 Jan 29;105(4):539-42
references.pmid: 11815441
references.citation: J Nucl Med. 2009 Jan;50(1):132-8
references.pmid: 19091898
references.citation: Am J Physiol Heart Circ Physiol. 2007 Jun;292(6):H3172-8
references.pmid: 17322414
references.citation: Phys Med Biol. 2005 Nov 21;50(22):R45-61
references.pmid: 16264248
references.citation: Phys Med Biol. 2008 Oct 7;53(19):R319-50
references.pmid: 18758005
references.citation: Magn Reson Med. 2010 Apr;63(4):979-87
references.pmid: 20373399
references.citation: NMR Biomed. 2007 May;20(3):360-5
references.pmid: 17451168
references.citation: Int J Cardiovasc Imaging. 2011 Jul;27(6):787-94
references.pmid: 20972707