title: Two-dimensional high-performance thin-layer chromatography of tryptic bovine albumin digest using normal- and reverse-phase systems with silanized silica stationary phase.
abstract: Among many advantages of planar techniques, two-dimensional (2D) separation seems to be the most important for analysis of complex samples. Here we present quick, simple and efficient two-dimensional high-performance thin-layer chromatography (2D HPTLC) of bovine albumin digest using commercial HPTLC RP-18W plates (silica based stationary phase with chemically bonded octadecyl ligands of coverage density 0.5μmol/m(2) from Merck, Darmstadt). We show, that at low or high concentration of water in the mobile phase comprised methanol and some additives the chromatographic systems with the plates mentioned demonstrate normal- or reversed-phase liquid chromatography properties, respectively, for separation of peptides obtained. These two systems show quite different separation selectivity and their combination into 2D HPTLC process provides excellent separation of peptides of the bovine albumin digest.
authors.affiliation: Department of Physical Chemistry, Chair of Chemistry, Medical University of Lublin, Chodźki Street 4A, 20-093 Lublin, Poland. Electronic address: radoslaw.gwarda@umlub.pl.
authors.forename: Radosław Łukasz
authors.initials: RŁ
authors.lastname: Gwarda
authors.forename: Tadeusz Henryk
authors.initials: TH
authors.lastname: Dzido
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D008956:Models, Chemical; D010446:Peptide Fragments / Q000032:analysis* / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D014357:Trypsin
chemical_list: D010446:Peptide Fragments; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D014357:Trypsin
keywords: Protein digest separation; Thin-layer chromatography; Two-dimensional chromatography