title: Production of pullulan by a thermotolerant aureobasidium pullulans strain in non-stirred fed batch fermentation process.
abstract: Total 95 isolates of Aureobasidium pullulans were isolated from different flowers and leaves samples, out of which 11 thermotolerant strains produced pullulan. One thermotolerant non-melanin pullulan producing strain, designated as RG-5, produced highest pullulan (37.1±1.0 g/l) at 42(o)C, pH 5.5 in 48h of incubation with 3% sucrose and 0.5% ammonium sulphate in a non-stirred fed batch fermentor of 6 liters capacity. The two liters of initial volume of fermentation medium was further fed with the 2 liters in two successive batches at 5 h interval into the fermentor. The sterile air was supplied only for 10h at the rate of 0.5 vvm.
authors.affiliation: Amity Institute of Microbiol Biotechnology (AIMB), Amity University , Noida (U.P.) , Índia.
authors.forename: Ranjan
authors.initials: R
authors.lastname: Singh
authors.forename: Rajeeva
authors.initials: R
authors.lastname: Gaur
authors.forename: Soni
authors.initials: S
authors.lastname: Tiwari
authors.forename: Manogya Kumar
authors.initials: MK
authors.lastname: Gaur
keywords: Aureobasidium pullulans; Non-stirred fed-batch fermentation; Pullulan
references.citation: FEMS Microbiol Lett. 2004 Mar 19;232(2):225-8
references.pmid: 15033243
references.citation: Appl Environ Microbiol. 1999 Dec;65(12):5265-71
references.pmid: 10583975
references.citation: Carbohydr Res. 2004 Feb 25;339(3):447-60
references.pmid: 15013381
references.citation: Appl Environ Microbiol. 1992 Mar;58(3):877-83
references.pmid: 16348676
references.citation: J Appl Microbiol. 2010 Sep;109(3):1035-43
references.pmid: 20456529
references.citation: World J Microbiol Biotechnol. 1994 Jul;10(4):423-6
references.pmid: 24421090
references.citation: Appl Biochem Biotechnol. 2002 Jan;97(1):1-22
references.pmid: 11900113