title: Cariogenic bacteria degrade dental resin composites and adhesives.
abstract: A major reason for dental resin composite restoration replacement is related to secondary caries promoted by acid production from bacteria including Streptococcus mutans (S. mutans). We hypothesized that S. mutans has esterase activities that degrade dental resin composites and adhesives. Standardized specimens of resin composite (Z250), total-etch (Scotchbond Multipurpose, SB), and self-etch (Easybond, EB) adhesives were incubated with S. mutans UA159 or uninoculated culture medium (control) for up to 30 days. Quantification of the BisGMA-derived biodegradation by-product, bishydroxy-propoxy-phenyl-propane (BisHPPP), was performed by high-performance liquid chromatography. Surface analysis of the specimens was performed by scanning electron microscopy (SEM). S. mutans was shown to have esterase activities in levels comparable with those found in human saliva. A trend of increasing BisHPPP release throughout the incubation period was observed for all materials and was more elevated in the presence of bacteria vs. control medium for EB and Z250, but not for SB (p < .05). SEM confirmed the increased degradation of all materials with S. mutans UA159 vs. control. S. mutans has esterase activities at levels that degrade resin composites and adhesives; degree of degradation was dependent on the material's chemical formulation. This finding suggests that the resin-dentin interface could be compromised by oral bacteria that contribute to the progression of secondary caries.
authors.affiliation: Dental Research Institute, Faculty of Dentistry, University of Toronto, ON, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Bourbia
authors.forename: D
authors.initials: D
authors.lastname: Ma
authors.forename: D G
authors.initials: DG
authors.lastname: Cvitkovitch
authors.forename: J P
authors.initials: JP
authors.lastname: Santerre
authors.forename: Y
authors.initials: Y
authors.lastname: Finer
mesh_terms: D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003188:Composite Resins / Q000737:chemistry*; D003470:Culture Media; D003731:Dental Caries / Q000382:microbiology*; D003764:Dental Materials / Q000737:chemistry*; D004950:Esterases / Q000378:metabolism; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D056951:Photoelectron Spectroscopy; D011092:Polyethylene Glycols / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013295:Streptococcus mutans / Q000201:enzymology / Q000378:metabolism*; D013499:Surface Properties; D017155:Wettability
chemical_list: D003188:Composite Resins; D003470:Culture Media; D003764:Dental Materials; C444701:Filtek Z250; D008689:Methacrylates; D011109:Polymethacrylic Acids; D011140:Polyurethanes; D019279:Resin Cements; C084018:Scotchbond Multi-Purpose; C029824:urethane dimethacrylate luting resin; C020946:triethylene glycol dimethacrylate; C041979:2,2-bis-(4-(2-methacryloxyethoxy)phenyl)propane; D011092:Polyethylene Glycols; D017438:Bisphenol A-Glycidyl Methacrylate; C005044:hydroxyethyl methacrylate; D004950:Esterases
keywords: Streptococcus mutans; biodegradation; chromatography; dental bonding; esterases; hydrolysis
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