title: Antibacterial activities of multi drug resistant Myroides odoratimimus bacteria isolated from adult flesh flies (Diptera: sarcophagidae) are independent of metallo beta-lactamase gene.
abstract: Sarcophagidae) are well known cause of myiasis and their gut bacteria have never been studied for antimicrobial activity against bacteria. Antimicrobial studies of Myroides spp. are restricted to nosocomial strains. A Gram-negative bacterium, Myroides sp., was isolated from the gut of adult flesh flies (Sarcophaga sp.) and submitted to evaluation of nutritional parameters using Biolog GN, 16S rRNA gene sequencing, susceptibility to various antimicrobials by disc diffusion method and detection of metallo β-lactamase genes (TUS/MUS). The antagonistic effects were tested on Gram-negative and Gram-positive bacteria isolated from human clinical specimens, environmental samples and insect mid gut. Bacterial species included were Aeromonas hydrophila, A. culicicola, Morganella morganii subsp. sibonii, Ochrobactrum anthropi, Weissella confusa, Escherichia coli, Ochrobactrum sp., Serratia sp., Kestersia sp., Ignatzschineria sp., Bacillus sp. The Myroides sp. strain was resistant to penicillin-G, erythromycin, streptomycin, amikacin, kanamycin, gentamycin, ampicillin, trimethoprim and tobramycin. These strain showed antibacterial action against all bacterial strains except W. confusa, Ignatzschineria sp., A. hydrophila and M. morganii subsp. sibonii. The multidrug resistance of the strain was similar to the resistance of clinical isolates, inhibiting growth of bacteria from clinical, environmental and insect gut samples. The metallo β-lactamase (TUS/MUS) genes were absent, and resistance due to these genes was ruled out, indicating involvement of other secretion machinery.
authors.affiliation: Molecular Biology Unit, National Centre for Cell Science, Ganeshkhind , Pune-411 007, Maharashtra , India ; Present address: Produce and Quality and Safety Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service , 10300 Baltimore Avenue, Beltsville, Maryland 20705 , United States.
authors.forename: M S
authors.initials: MS
authors.lastname: Dharne
authors.forename: A K
authors.initials: AK
authors.lastname: Gupta
authors.forename: A Y
authors.initials: AY
authors.lastname: Rangrez
authors.forename: H V
authors.initials: HV
authors.lastname: Ghate
authors.forename: M S
authors.initials: MS
authors.lastname: Patole
authors.forename: Y S
authors.initials: YS
authors.lastname: Shouche
keywords: 16S rRNA; Flesh fly; Myroides sp.; antibacterial activity; metallo beta-lactamase
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