title: Quantitative analysis of microRNA in blood serum with protein-facilitated affinity capillary electrophoresis.
abstract: MicroRNAs play an important role in gene regulation and disease etiology and are blood-based biomarkers of diseases. Here, we describe a protein-facilitated affinity capillary electrophoresis (ProFACE) method for ultra-sensitive direct miRNA detection as low as 300,000 molecules in 1 mL of blood serum, using single-stranded DNA binding protein (SSB) and double-stranded RNA binding protein (p19) as separation enhancers. This method utilizes either the selective binding of SSB to a fluorescent single-stranded DNA/RNA probe or the binding of p19 to miRNA-RNA probe duplex.
authors.affiliation: University of Ottawa, Ottawa, ON, Canada.
authors.forename: Maxim V
authors.initials: MV
authors.lastname: Berezovski
authors.forename: Nasrin
authors.initials: N
authors.lastname: Khan
mesh_terms: D015374:Biosensing Techniques; D001774:Blood Chemical Analysis; D004268:DNA-Binding Proteins / Q000737:chemistry; D019075:Electrophoresis, Capillary; D006801:Humans; D035683:MicroRNAs / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D015347:RNA Probes / Q000737:chemistry; D012330:RNA, Double-Stranded / Q000737:chemistry; D016601:RNA-Binding Proteins / Q000737:chemistry
chemical_list: D004268:DNA-Binding Proteins; D035683:MicroRNAs; D015347:RNA Probes; D012330:RNA, Double-Stranded; D016601:RNA-Binding Proteins