title: Molecular genotyping of Echinococcus granulosus from dromedaries (Camelus dromedarius) in eastern Iran.
abstract: With the aim of genotyping Echinococcus granulosus cysts found in Iranian dromedaries (Camelus dromedarius), 50 cysts of E. granulosus were collected from five geographical regions in Iran. Cysts were characterized using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the internal transcribed spacer 1 (ITS1) gene and sequencing fragments of the genes coding for mitochondrial cytochrome c oxidase subunit 1 (cox1). Morphological criteria using rostellar hook dimensions were also undertaken. The present results have shown that 27 out of 50 E. granulosus cysts (54%) were determined as the G1 strain, and the other (46%) were determined as the G6 strain. The molecular analysis of the ITS1 region of ribosomal DNA corresponded with the morphological findings. Because of its recognized infectivity in humans, the G1 genotype is a direct threat to human health and its presence in Iranian dromedaries is of urgent public health importance.
authors.affiliation: Department of Pathobiology,School of Veterinary Medicine, Ferdowsi University of Mashhad,Mashhad,Iran.
authors.forename: E
authors.initials: E
authors.lastname: Moghaddas
authors.affiliation: Department of Pathobiology,School of Veterinary Medicine, Ferdowsi University of Mashhad,Mashhad,Iran.
authors.forename: H
authors.initials: H
authors.lastname: Borji
authors.affiliation: Department of Pathobiology,School of Veterinary Medicine, Ferdowsi University of Mashhad,Mashhad,Iran.
authors.forename: A
authors.initials: A
authors.lastname: Naghibi
authors.affiliation: Department of Parasitology,School of Veterinary Medicine, Tehran University,Tehran,Iran.
authors.forename: P
authors.initials: P
authors.lastname: Shayan
authors.affiliation: Department of Pathobiology,School of Veterinary Medicine, Ferdowsi University of Mashhad,Mashhad,Iran.
authors.forename: G R
authors.initials: GR
authors.lastname: Razmi
mesh_terms: D000818:Animals; D002162:Camelus / Q000469:parasitology*; D018509:DNA, Helminth / Q000235:genetics; D004443:Echinococcosis / Q000469:parasitology / Q000662:veterinary*; D048209:Echinococcus granulosus / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D003576:Electron Transport Complex IV / Q000235:genetics; D005838:Genotype; D015801:Helminth Proteins / Q000235:genetics; D007492:Iran; D058889:Molecular Typing; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D018509:DNA, Helminth; D015801:Helminth Proteins; D003576:Electron Transport Complex IV