title: A study on the effect of Helicobacter pylori infection on p53 expression in gastric cancer and gastritis tissues.
abstract: BACKGROUND
  Helicobacter pylori cause damage to gastric epithelial cells and alterations in the p53 gene that lead to cancer development. This study aimed to determine the correlation of p53 expression with H. pylori using immunohistochemistry, RFLP-PCR, and histopathology.
  
  
  METHODS
  Gastric biopsy samples from gastric cancer (GC) (n = 54) and gastritis (n = 31) patients were examined for histopathological changes and expression of p53 protein by immunohistochemistry.
  
  
  RESULTS
  Immunohistochemical analysis of p53 protein expression in H. pylori-positive GC sections showed an average of 44.3% positive cells in tumors and 6.9% in normal tissues, as compared to 16.4% and 4.4% in H. pylori-negative sections. P53 expression showed significant association with H. pylori (P = 0.005), invasion depth (P = 0.029) and inflammation reaction (P = 0.008). In gastritis sections, no difference in the average p53 staining in H. pylori-positive or -negative sections was seen. PCR-RFLP results also showed no difference in genotype frequencies of p53 in H. pylori-positive or -negative gastritis sections. Histopathology study of H. pylori-positive GC sections showed that 97.2% were the intestinal type and 2.8% the diffuse type, while in H. pylori-negative sections 35.2% were the intestinal type and 64.8% the diffuse type. Biopsy sections from H. pylori-positive gastritis patients revealed more severe inflammation than those of H. pylori-negative patients.
  
  
  CONCLUSIONS
  Our results show that H. pylori infection affects p53 expression in GC. The average p53 expression was significantly higher in tumor than in normal tissues. In gastritis sections p53 expression was significantly associated with H. pylori.
authors.affiliation: Faculty of Science, Fatih University, Istanbul, Turkey. basalih@fatih.edu.tr.
authors.forename: Barik A
authors.initials: BA
authors.lastname: Salih
authors.forename: Zuhal
authors.initials: Z
authors.lastname: Gucin
authors.forename: Nizamettin
authors.initials: N
authors.lastname: Bayyurt
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D005260:Female; D005756:Gastritis / Q000473:pathology*; D020869:Gene Expression Profiling; D005838:Genotype; D016481:Helicobacter Infections / Q000150:complications* / Q000473:pathology*; D016480:Helicobacter pylori / Q000302:isolation & purification; D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013274:Stomach Neoplasms / Q000473:pathology*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000235:genetics; D055815:Young Adult
chemical_list: D016159:Tumor Suppressor Protein p53