title: The effect of the adaptor protein Isd11 on the quaternary structure of the eukaryotic cysteine desulphurase Nfs1.
abstract: Small inorganic assemblies of alternating ferrous/ferric iron and sulphide ions, so-called iron-sulphur (Fe-S) clusters, are possibly nature's most ancient prosthetic groups. One of the early actors in Fe-S cluster biosynthesis is a protein complex composed of a cysteine desulphurase, Nfs1, and its functional binding partner, Isd11. Although the essential function of Nfs1·Isd11 in the liberation of elemental sulphur from free cysteine is well established, little is known about its structure. Here, we provide evidence that shows Isd11 has a profound effect on the oligomeric state of Nfs1.
authors.affiliation: School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK.
authors.forename: Kerem
authors.initials: K
authors.lastname: Terali
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Beavil
authors.forename: Richard W
authors.initials: RW
authors.lastname: Pickersgill
authors.forename: Mark
authors.initials: M
authors.lastname: van der Giezen
mesh_terms: D002942:Circular Dichroism; D007506:Iron-Sulfur Proteins / Q000737:chemistry*; D024101:Mitochondrial Proteins / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D020836:Protein Structure, Quaternary; D011994:Recombinant Proteins / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D040681:Structural Homology, Protein; D013466:Sulfurtransferases / Q000737:chemistry*
chemical_list: D007506:Iron-Sulfur Proteins; C508374:Isd11 protein, S cerevisiae; D024101:Mitochondrial Proteins; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D013466:Sulfurtransferases; C115912:NFS1 protein, S cerevisiae
keywords: Iron–sulphur (Fe–S) cluster assembly; Isd11; Mitochondria; Nfs1; Saccharomyces cerevisiae