title: The GLI1 splice variant TGLI1 promotes glioblastoma angiogenesis and growth.
abstract: We investigated truncated glioma-associated oncogene homolog 1 (TGLI1) that behaves as gain-of-function GLI1 and promotes tumor cell migration and invasion. Herein, we report that TGLI1 had a higher propensity than GLI1 to enhance glioblastoma angiogenesis and growth, both in vivo and in vitro. TGLI1 has gained the ability to enhance expression of pro-angiogenic heparanase. In patient glioblastomas, TGLI1 levels are correlated with heparanase expression. Together, we report that TGLI1 is a novel mediator of glioblastoma angiogenesis and that heparanase is a novel transcriptional target of TGLI1, shedding new light on the molecular pathways that support tumor angiogenesis and aggressive growth.
authors.affiliation: Department of Surgery, Division of Surgical Sciences, Durham, NC 27710, USA.
authors.forename: Hu
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Surgery, Division of Surgical Sciences, Durham, NC 27710, USA.
authors.forename: Richard L
authors.initials: RL
authors.lastname: Carpenter
authors.affiliation: Department of Surgery, Division of Surgical Sciences, Durham, NC 27710, USA.
authors.forename: Woody
authors.initials: W
authors.lastname: Han
authors.affiliation: Department of Surgery, Division of Surgical Sciences, Durham, NC 27710, USA; Duke Center for RNA Biology, Durham, NC 27710, USA; Duke Cancer Institute, Duke University School of Medicine, Durham, NC 27710, USA. Electronic address: huiwen.lo@duke.edu.
authors.forename: Hui-Wen
authors.initials: HW
authors.lastname: Lo
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002642:Chick Embryo; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology*; D005966:Glucuronidase / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008833:Microcirculation; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D000071676:Zinc Finger Protein GLI1; D000099175:Heparanase
chemical_list: D005966:Glucuronidase; D014157:Transcription Factors; D000071676:Zinc Finger Protein GLI1; D000099175:Heparanase; C400551:GLI1 protein, human
keywords: Angiogenesis; Cancer; GLI1; Hedgehog pathway; Transcriptional regulation
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