title: Supplementation with n-3 polyunsaturated fatty acids to lipopolysaccharide-induced rats improved inflammation and functional properties of renal Na,K-ATPase.
abstract: Measurements of enzyme kinetics of renal Na, K-ATPase were used for characterization of ATP- and Na⁺-binding sites in rats that were subjected to 10 days of moderate inflammation that was induced by a single dose of Escherichia coli lipopolysaccharides (LPSs) at a dose of 1 mg kg⁻¹ body weight. We hypothesized that LPSs might initiate a malfunction of renal Na, K-ATPase, which is a key enzyme involved in regulation of sodium homeostasis in the organism. We also investigated the potential effect that fish oil (FO) has in the prevention of Na, K-ATPase alterations by administering FO daily at a dose of 30 mg kg⁻¹. Alone, LPS elevated the level of C-reactive protein by more than 500% and free radicals by 36% in plasma, as indicated by an increased level of malondialdehyde. The Na, K-ATPase was slightly altered in the vicinity of the ATP-binding site as suggested by the 9% increase of the concentration of ATP necessary for half-maximal activation of the enzyme, thus indicating a deteriorated binding of ATP as a consequence of inflammation. Daily supplementation of FO partly attenuated LPS-induced injury, as observed by a significant decrease in the plasma levels of C-reactive protein and free radicals, hence maintaining the activity of renal Na, K-ATPase to the level of healthy control animals. In conclusion, our findings showed that FO prevented an excessive malondialdehyde production in LPS-treated animals and stabilized renal Na, K-ATPase.
authors.affiliation: Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: Lucia
authors.initials: L
authors.lastname: Mézešová
authors.forename: Veronika
authors.initials: V
authors.lastname: Jendruchová-Javorková
authors.forename: Jana
authors.initials: J
authors.lastname: Vlkovičová
authors.forename: L'udmila
authors.initials: L
authors.lastname: Okruhlicová
authors.forename: Karel
authors.initials: K
authors.lastname: Frimmel
authors.forename: Jana
authors.initials: J
authors.lastname: Navarová
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Kyselová-Brnoliaková
authors.forename: Norbert
authors.initials: N
authors.lastname: Vrbjar
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D002097:C-Reactive Protein / Q000378:metabolism; D019587:Dietary Supplements; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage*; D005395:Fish Oils / Q000008:administration & dosage; D006339:Heart Rate / Q000187:drug effects; D007249:Inflammation / Q000188:drug therapy; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology; D008070:Lipopolysaccharides / Q000009:adverse effects*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D008070:Lipopolysaccharides; D008315:Malondialdehyde; D002097:C-Reactive Protein; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase
keywords: C; DHAs; FO; Fish oil; HR; Inflammation; K(m); Kidney; LPS; MDA; NaCl; ROS; Rat; SBP; Sodium pump; V(max); concentration of ATP necessary for half-maximal activation of the enzyme; control group; docosahexaenoic acid; fish oil; heart rate; lipopolysaccharides; malondialdehyde; maximum velocity; n-3 PUFA; polyunsaturated fatty acid; reactive oxygen species; sodium chloride; systolic blood pressure