title: A RT-PCR assay for the detection of encephalomycarditis virus infections in pigs.
abstract: Encephalomyocarditis virus (EMCV) infections can cause losses in pig farms all over the world. Rapid, sensitive and unequivocal detection of this virus is therefore essential for the diagnosis and control of the disease. An RT-PCR assay was developed, optimized and evaluated for encephalomyocarditis virus detection in organ based on a pair of primers that amplifies a 165 bp DNA fragment from a highly conserved nucleotide region of the viral 3D glycoprotein. PCR products of the expected size were obtained from Cuban EMCV 744/03 strain. Non-specific reactions were not observed when other porcine RNA genome viruses and uninfected cells were used. The analytical sensitivity of the test was estimated to be 2 TCID50/50 μL. The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases.
authors.affiliation: Grupo de Virología, Centro Nacional de Sanidad Agropecuaria. San José de las Lajas, La Habana , Cuba.
authors.forename: Lester J
authors.initials: LJ
authors.lastname: Pérez
authors.forename: Heidy
authors.initials: H
authors.lastname: Díaz de Arce
keywords: Encephalomyocarditis virus; RT-PCR assay; pig
references.citation: J Virol Methods. 2008 Feb;147(2):301-11
references.pmid: 17964668
references.citation: Zentralbl Veterinarmed B. 1993 Jul;40(5):347-52
references.pmid: 8237207
references.citation: Arch Virol. 2003 May;148(5):903-12
references.pmid: 12721798
references.citation: J Clin Microbiol. 1998 Dec;36(12):3463-7
references.pmid: 9817855
references.citation: J Virol Methods. 1997 Jun;66(1):83-9
references.pmid: 9220393
references.citation: Vet Microbiol. 2006 Mar 10;113(1-2):1-12
references.pmid: 16406410
references.citation: Proc Natl Acad Sci U S A. 2008 Sep 16;105(37):14124-9
references.pmid: 18768820
references.citation: Acta Vet Hung. 2005;53(1):113-24
references.pmid: 15782664
references.citation: Nucleic Acids Res. 1995 Feb 11;23(3):377-82
references.pmid: 7885833
references.citation: Med J Aust. 1989 Aug 7;151(3):176, 178
references.pmid: 2547141
references.citation: Can J Vet Res. 1990 Oct;54(4):457-64
references.pmid: 2249179
references.citation: Vaccine. 2007 Jul 26;25(30):5444-52
references.pmid: 17224207
references.citation: Am J Vet Res. 1992 Jan;53(1):44-7
references.pmid: 1311538
references.citation: Vet Microbiol. 1999 Dec;70(3-4):179-92
references.pmid: 10596802
references.citation: J Virol Methods. 2002 Mar;101(1-2):197-206
references.pmid: 11849698
references.citation: Epidemiol Infect. 2005 Jun;133(3):547-57
references.pmid: 15962562
references.citation: Virus Res. 1998 Sep;57(1):53-62
references.pmid: 9833886
references.citation: Xenotransplantation. 2003 Nov;10(6):569-76
references.pmid: 14708524