title: Differentially expressed genes of Coptotermes formosanus (Isoptera: Rhinotermitidae) challenged by chemical insecticides.
abstract: Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae) termites are harmful social insects to wood constructions. The current control methods heavily depend on the chemical insecticides with increasing resistance. Analysis of the differentially expressed genes mediated by chemical insecticides will contribute to the understanding of the termite resistance to chemicals and to the establishment of alternative control measures. In the present article, a full-length cDNA library was constructed from the termites induced by a mixture of commonly used insecticides (0.01% sulfluramid and 0.01% triflumuron) for 24 h, by using the RNA ligase-mediated Rapid Amplification cDNA End method. Fifty-eight differentially expressed clones were obtained by polymerase chain reaction and confirmed by dot-blot hybridization. Forty-six known sequences were obtained, which clustered into 33 unique sequences grouped in 6 contigs and 27 singlets. Sixty-seven percent (22) of the sequences had counterpart genes from other organisms, whereas 33% (11) were undescribed. A Gene Ontology analysis classified 33 unique sequences into different functional categories. In general, most of the differential expression genes were involved in binding and catalytic activity.
authors.affiliation: Research Centre for Insect Resources and Biotechnology, Guangdong Entomological Institute, Xing Gong Rd., West 105, 510260 Guangzhou, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xuehong
authors.initials: X
authors.lastname: Qiu
authors.forename: Richou
authors.initials: R
authors.lastname: Han
mesh_terms: D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D018076:DNA, Complementary / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D005466:Fluorocarbons / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007306:Insecticides / Q000494:pharmacology*; D020049:Isoptera / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D021141:Nucleic Acid Amplification Techniques; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D013449:Sulfonamides / Q000494:pharmacology*
chemical_list: D001549:Benzamides; D018076:DNA, Complementary; D005466:Fluorocarbons; D007306:Insecticides; D012333:RNA, Messenger; D013449:Sulfonamides; C023957:triflumuron; C063732:sulfluramid