title: Massilia umbonata sp. nov., able to accumulate poly-β-hydroxybutyrate, isolated from a sewage sludge compost-soil microcosm.
abstract: A bacterial strain, designated strain LP01(T), was isolated from a laboratory-scale microcosm packed with a mixture of soil and sewage sludge compost designed to study the evolution of microbial biodiversity over time. The bacterial strain was selected for its potential ability to store polyhydroxyalkanoates (PHAs) as intracellular granules. The cells were aerobic, Gram-stain-negative, non-endospore-forming motile rods. Phylogenetically, the strain was classified within the genus Massilia, as its 16S rRNA gene sequence had similarity of 99.2 % with respect to those of Massilia albidiflava DSM 17472(T) and M. lutea DSM 17473(T). DNA-DNA hybridization showed low relatedness of strain LP01(T) to the type strains of other, phylogenetically related species of the genus Massilia. It contained Q-8 as the predominant ubiquinone and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) as the major fatty acid(s). It was found to contain small amounts of the fatty acids C18 : 0 and C14 : 0 2-OH, a feature that served to distinguish it from its closest phylogenetic relatives within the genus Massilia. The DNA G+C content was 66.0 mol%. Phylogenetic, phenotypic and chemotaxonomic data obtained in this study suggest that strain LP01(T) represents a novel species of the genus Massilia, for which the name Massilia umbonata sp. nov. is proposed. The type strain is LP01(T) ( = CECT 7753(T) = DSM 26121(T)).
authors.affiliation: Max-Planck-Institut für Marine Mikrobiologie, Celsiusstrasse 1, 28359 Bremen, Germany.|Department of Microbiology, University of Granada, Granada, Spain.
authors.forename: Marina
authors.initials: M
authors.lastname: Rodríguez-Díaz
authors.affiliation: Water Research Institute, University of Granada, Granada, Spain.
authors.forename: Federico
authors.initials: F
authors.lastname: Cerrone
authors.affiliation: Water Research Institute, University of Granada, Granada, Spain.
authors.forename: Mar
authors.initials: M
authors.lastname: Sánchez-Peinado
authors.affiliation: Water Research Institute, University of Granada, Granada, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: SantaCruz-Calvo
authors.affiliation: Water Research Institute, University of Granada, Granada, Spain.|Department of Microbiology, University of Granada, Granada, Spain.
authors.forename: Clementina
authors.initials: C
authors.lastname: Pozo
authors.affiliation: Water Research Institute, University of Granada, Granada, Spain.|Department of Microbiology, University of Granada, Granada, Spain.
authors.forename: Jesús González
authors.initials: JG
authors.lastname: López
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006885:Hydroxybutyrates / Q000378:metabolism*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D042802:Oxalobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D010802:Phylogeny; D011091:Polyesters / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D012987:Soil; D012988:Soil Microbiology; D013030:Spain; D014451:Ubiquinone / Q000737:chemistry; D000094122:Polyhydroxybutyrates
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006885:Hydroxybutyrates; D011091:Polyesters; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D012987:Soil; D014451:Ubiquinone; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C030778:ubiquinone 8