title: Integrative analysis of porcine microRNAome during skeletal muscle development.
abstract: Pig is an important agricultural animal for meat production and provides a valuable model for many human diseases. Functional studies have demonstrated that microRNAs (miRNAs) play critical roles in almost all aspects of skeletal muscle development and disease pathogenesis. To investigate the miRNAs involved in regulating different periods of skeletal muscle development, we herein performed a comprehensive research for porcine microRNAome (miRNAome) during 10 skeletal muscle developmental stages including 35, 49, 63, 77, 91 dpc (days post coitum) and 2, 28, 90, 120, 180 dpn (days postnatal) using Solexa sequencing technology. Our results extend the repertoire of pig miRNAome to 247 known miRNAs processed from 210 pre-miRNAs and 297 candidate novel miRNAs through comparison with known miRNAs in the miRBase. Expression analysis of the 15 most abundant miRNAs in every library indicated that functional miRNAome may be smaller and tend to be highly expressed. A series of muscle-related miRNAs summarized in our study present different patterns between myofibers formation phase and muscle maturation phase, providing valuable reference for investigation of functional miRNAs during skeletal muscle development. Analysis of temporal profiles of miRNA expression identifies 18 novel candidate myogenic miRNAs in pig, which might provide new insight into regulation mechanism mediated by miRNAs underlying muscle development.
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Qin
authors.forename: Yaosheng
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Liu
authors.forename: Sanxing
authors.initials: S
authors.lastname: Ye
authors.forename: Kaifan
authors.initials: K
authors.lastname: Yu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Huang
authors.forename: Jingwei
authors.initials: J
authors.lastname: Yu
authors.forename: Xingyu
authors.initials: X
authors.lastname: Zhou
authors.forename: Hu
authors.initials: H
authors.lastname: Chen
authors.forename: Delin
authors.initials: D
authors.lastname: Mo
mesh_terms: D000818:Animals; D030541:Databases, Genetic; D005260:Female; D018507:Gene Expression Regulation, Developmental; D055029:Inverted Repeat Sequences; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D034421:Sus scrofa / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism; D059467:Transcriptome
chemical_list: D035683:MicroRNAs
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