title: Dynamic proteomics of nucleus accumbens in response to acute psychological stress in environmentally enriched and isolated rats.
abstract: Our prior research has shown that environmental enrichment (i.e. rats reared in an environment with novel objects, social contact with conspecifics) produces a protective antidepressant-like phenotype in rats and decreases neurobiological effects of acute psychological stress. Although CREB activity has been identified as a major player, the downstream molecular mechanisms remain largely unexplored. Thus, the current study investigates proteomic differences in the accumbens of rats raised in an enriched condition (EC) versus those raised in an isolated control condition (IC) under basal conditions and after 30 min of acute restraint stress. Results showed that under basal conditions, EC rats generally expressed less mitochondria-related proteins, particularly those involved in TCA cycle and electron transport compared to IC rats. After 30 min of acute stress, EC rats displayed increased expression of energy metabolism enzymes (among others) while IC rats exhibited decreased expression of similar proteins. Further, network and pathway analyses also identified links to AKT signaling proteins, 14-3-3 family proteins, heat-shock proteins, and ubiquitin-interacting proteins. The protein ENO1 showed marked differential expression and regulation; EC rats expressed higher levels under basal conditions that increased subsequent to stress, while the basal IC expression was lower and decreased further still after stress. The results of this study define differential protein expression in a protective rat model for major depression and additionally identify a dynamic and coordinated differential response to acute stress between the two groups. These results provide new avenues for exploration of the molecular determinants of depression and the response to acute stress.
authors.affiliation: Center for Addiction Research, Department of Pharmacology and Toxicology, The University of Texas Medical Branch, Galveston, Texas, United States of America.
authors.forename: Xiuzhen
authors.initials: X
authors.lastname: Fan
authors.forename: Dingge
authors.initials: D
authors.lastname: Li
authors.forename: Cheryl F
authors.initials: CF
authors.lastname: Lichti
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Green
mesh_terms: D000818:Animals; D015153:Blotting, Western; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D007700:Kinetics; D008297:Male; D008954:Models, Biological; D009714:Nucleus Accumbens / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical / Q000009:adverse effects; D015398:Signal Transduction; D012931:Social Environment; D012934:Social Isolation; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013315:Stress, Psychological / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein
references.citation: PLoS One. 2012;7(8):e44149
references.pmid: 22952905
references.citation: J Neurosci. 2006 Aug 9;26(32):8235-42
references.pmid: 16899718
references.citation: Nat Med. 2010 Nov;16(11):1328-32
references.pmid: 20953200
references.citation: Nat Neurosci. 2009 Feb;12(2):200-9
references.pmid: 19151710
references.citation: Pharmacol Biochem Behav. 1992 Apr;41(4):817-24
references.pmid: 1594650
references.citation: J Biol Chem. 2008 May 23;283(21):14751-61
references.pmid: 18332147
references.citation: Brain Res. 1986 Nov 12;397(2):395-400
references.pmid: 3801880
references.citation: J Neurosci. 2011 Nov 9;31(45):16447-57
references.pmid: 22072694
references.citation: CNS Spectr. 2007 Jun;12(6):429-38
references.pmid: 17545953
references.citation: Nat Cell Biol. 2005 Oct;7(10):999-1006
references.pmid: 16179952
references.citation: Biol Psychiatry. 2006 Jun 15;59(12):1151-9
references.pmid: 16566899
references.citation: Br J Pharmacol. 2000 Jul;130(5):1115-23
references.pmid: 10882397
references.citation: Nature. 2007 Jan 11;445(7124):168-76
references.pmid: 17151600
references.citation: Biochem Biophys Res Commun. 2011 Oct 7;413(4):550-4
references.pmid: 21925151
references.citation: J Biol Chem. 2011 Oct 28;286(43):37778-92
references.pmid: 21832084
references.citation: J Affect Disord. 2003 Sep;76(1-3):55-68
references.pmid: 12943934
references.citation: Science. 1998 Dec 18;282(5397):2272-5
references.pmid: 9856954
references.citation: Biol Psychiatry. 2010 Jan 1;67(1):28-35
references.pmid: 19709647
references.citation: J Neurosci. 2008 Feb 27;28(9):2025-32
references.pmid: 18305237
references.citation: Channels (Austin). 2011 Sep-Oct;5(5):449-56
references.pmid: 21829088
references.citation: Neuropharmacology. 1993 Sep;32(9):885-93
references.pmid: 8232791
references.citation: Psychopharmacology (Berl). 2002 Aug;162(4):373-8
references.pmid: 12172690
references.citation: J Neurochem. 2001 May;77(3):916-28
references.pmid: 11331420
references.citation: J Neurosci Res. 2004 Feb 15;75(4):451-60
references.pmid: 14743428
references.citation: BMC Musculoskelet Disord. 2011 Oct 17;12:237
references.pmid: 22004293
references.citation: Proc Natl Acad Sci U S A. 2005 Mar 22;102(12):4459-64
references.pmid: 15753290
references.citation: Semin Cell Dev Biol. 2011 Sep;22(7):696-704
references.pmid: 21920445
references.citation: Trends Neurosci. 2010 Jun;33(6):267-76
references.pmid: 20207024
references.citation: J Biol Chem. 2009 Nov 6;284(45):31375-90
references.pmid: 19755421
references.citation: Neuroscience. 2006;137(4):1237-46
references.pmid: 16338082
references.citation: Psychopharmacology (Berl). 2011 Nov;218(1):293-301
references.pmid: 21887496
references.citation: Toxicol Appl Pharmacol. 2011 Aug 15;255(1):103-12
references.pmid: 21708182
references.citation: Mol Cell Proteomics. 2011 Oct;10(10):M111.013284
references.pmid: 21890473
references.citation: J Neurochem. 2007 Nov;103(3):1132-44
references.pmid: 17683481
references.citation: Arch Gen Psychiatry. 2005 Jun;62(6):593-602
references.pmid: 15939837
references.citation: Brain Res. 1997 Nov 21;776(1-2):61-7
references.pmid: 9439796
references.citation: Electrophoresis. 2010 Oct;31(21):3573-9
references.pmid: 20967768
references.citation: Curr Top Dev Biol. 2005;68:281-315
references.pmid: 16125003
references.citation: Behav Brain Res. 2002 Feb 1;129(1-2):41-50
references.pmid: 11809493
references.citation: Proteomics. 2006 Jun;6(11):3414-25
references.pmid: 16637010
references.citation: Neurochem Int. 2011 Jul;58(8):904-13
references.pmid: 21349309
references.citation: Neuron. 2011 Aug 11;71(3):498-511
references.pmid: 21835346
references.citation: Biochem Pharmacol. 2001 Dec 15;62(12):1597-603
references.pmid: 11755112
references.citation: Genes Cells. 2005 Feb;10(2):165-79
references.pmid: 15676027
references.citation: J Biotechnol. 2006 Dec 1;126(4):582-6
references.pmid: 16956687
references.citation: Prog Neuropsychopharmacol Biol Psychiatry. 2011 Apr 29;35(3):730-43
references.pmid: 20691744
references.citation: J Proteome Res. 2010 Mar 5;9(3):1522-32
references.pmid: 20108985
references.citation: Free Radic Biol Med. 1999 Aug;27(3-4):262-70
references.pmid: 10468197
references.citation: Behav Brain Res. 2011 Nov 20;225(1):135-41
references.pmid: 21767575
references.citation: Behav Brain Res. 2004 Jan 5;148(1-2):107-17
references.pmid: 14684252
references.citation: Cell. 1996 Mar 22;84(6):889-97
references.pmid: 8601312
references.citation: BMC Bioinformatics. 2011;12 Suppl 13:S20
references.pmid: 22373040
references.citation: Neuroscience. 1995 Feb;64(3):675-85
references.pmid: 7715780
references.citation: Neuropharmacology. 2011 Jun;60(7-8):1243-53
references.pmid: 21195720
references.citation: Physiol Behav. 1992 Apr;51(4):667-72
references.pmid: 1594664
references.citation: Nat Neurosci. 2010 Oct;13(10):1161-9
references.pmid: 20877280
references.citation: Biochem J. 1992 Jan 1;281 ( Pt 1):21-40
references.pmid: 1731757
references.citation: Microbiol Mol Biol Rev. 2008 Mar;72(1):197-210, table of contents
references.pmid: 18322039
references.citation: Pharmacol Biochem Behav. 1994 Jun;48(2):459-64
references.pmid: 8090815
references.citation: Am Fam Physician. 2009 Jul 15;80(2):167-72
references.pmid: 19621857