title: Principles and applications of polymerase chain reaction in medical diagnostic fields: a review.
abstract: Recent developments in molecular methods have revolutionized the detection and characterization of microorganisms in a broad range of medical diagnostic fields, including virology, mycology, parasitology, microbiology and dentistry. Among these methods, Polymerase Chain Reaction (PCR) has generated great benefits and allowed scientific advancements. PCR is an excellent technique for the rapid detection of pathogens, including those difficult to culture. Along with conventional PCR techniques, Real-Time PCR has emerged as a technological innovation and is playing an ever-increasing role in clinical diagnostics and research laboratories. Due to its capacity to generate both qualitative and quantitative results, Real-Time PCR is considered a fast and accurate platform. The aim of the present literature review is to explore the clinical usefulness and potential of both conventional PCR and Real-Time PCR assays in diverse medical fields, addressing its main uses and advances.
authors.affiliation: Universidade Federal de Pernambuco , Recife, PE , Brasil.
authors.forename: Marcela Agne Alves
authors.initials: MA
authors.lastname: Valones
authors.forename: Rafael Lima
authors.initials: RL
authors.lastname: Guimarães
authors.forename: Lucas André Cavalcanti
authors.initials: LA
authors.lastname: Brandão
authors.forename: Paulo Roberto Eleutério
authors.initials: PR
authors.lastname: de Souza
authors.forename: Alessandra
authors.initials: A
authors.lastname: de Albuquerque Tavares Carvalho
authors.forename: Sergio
authors.initials: S
authors.lastname: Crovela
keywords: Conventional PCR; Methods of molecular detection; Molecular Biology; Real-Time PCR
references.citation: Int J Oncol. 2001 Nov;19(5):1063-8
references.pmid: 11605010
references.citation: Periodontol 2000. 1999 Jun;20:53-64
references.pmid: 10522222
references.citation: Appl Environ Microbiol. 2004 Jan;70(1):167-73
references.pmid: 14711639
references.citation: J Clin Microbiol. 2000 Jun;38(6):2362-5
references.pmid: 10835003
references.citation: Genome Res. 1996 Oct;6(10):986-94
references.pmid: 8908518
references.citation: J Clin Microbiol. 2002 Jun;40(6):1985-8
references.pmid: 12037052
references.citation: Int Microbiol. 1998 Sep;1(3):217-20
references.pmid: 10943363
references.citation: Int J Cancer. 2000 May 20;89(3):286-92
references.pmid: 10861506
references.citation: J Med Microbiol. 2000 Oct;49(10):861-74
references.pmid: 11023183
references.citation: Br J Haematol. 2000 Nov;111(2):618-25
references.pmid: 11122110
references.citation: J Clin Microbiol. 2000 May;38(5):1981-3
references.pmid: 10790134
references.citation: Microbiol Immunol. 1996;40(11):867-71
references.pmid: 8985942
references.citation: Br J Haematol. 2000 Jan;108(1):132-9
references.pmid: 10651736
references.citation: J Appl Genet. 2004;45(1):3-15
references.pmid: 14960763
references.citation: Clin Diagn Virol. 1998 May 1;10(1):25-47
references.pmid: 9646000
references.citation: J Clin Microbiol. 2002 Dec;40(12):4423-7
references.pmid: 12454130
references.citation: Oral Microbiol Immunol. 1998 Aug;13(4):195-216
references.pmid: 10093535
references.citation: J Appl Oral Sci. 2004 Mar;12(1):1-11
references.pmid: 21365144
references.citation: Am J Pathol. 2000 Jun;156(6):1855-64
references.pmid: 10854209
references.citation: Microbiol Immunol. 2001;45(1):39-44
references.pmid: 11270605
references.citation: J Virol Methods. 1996 Apr 26;58(1-2):175-85
references.pmid: 8783163
references.citation: Nat Biotechnol. 1996 Mar;14(3):303-8
references.pmid: 9630890
references.citation: Clin Chem. 1998 May;44(5):918-23
references.pmid: 9590362
references.citation: Nucleic Acids Res. 2000 Jan 15;28(2):655-61
references.pmid: 10606668
references.citation: Proc Natl Acad Sci U S A. 1988 Dec;85(23):8790-4
references.pmid: 3194390
references.citation: Anal Biochem. 1994 Apr;218(1):1-13
references.pmid: 8053542
references.citation: Biotechniques. 1997 Jan;22(1):130-1, 134-8
references.pmid: 8994660
references.citation: Int Endod J. 1999 May;32(3):204-10
references.pmid: 10530208
references.citation: Clin Microbiol Infect. 2004 Jan;10(1):5-11
references.pmid: 14706081
references.citation: J Clin Microbiol. 1998 Jan;36(1):157-60
references.pmid: 9431940
references.citation: Biotechniques. 1998 Jun;24(6):954-8, 960, 962
references.pmid: 9631186
references.citation: Nat Biotechnol. 1998 Jan;16(1):49-53
references.pmid: 9447593
references.citation: J Clin Microbiol. 2002 Aug;40(8):2897-902
references.pmid: 12149348
references.citation: J Periodontal Res. 2000 Aug;35(4):232-41
references.pmid: 10983884
references.citation: Nat Biotechnol. 1999 Aug;17(8):804-7
references.pmid: 10429248
references.citation: J Clin Microbiol. 2001 Aug;39(8):3020-1
references.pmid: 11499395
references.citation: J Virol Methods. 2000 Sep;89(1-2):29-37
references.pmid: 10996637
references.citation: Science. 1998 Feb 20;279(5354):1228-9
references.pmid: 9508692
references.citation: J Clin Microbiol. 1999 Nov;37(11):3504-8
references.pmid: 10523542
references.citation: Cancer Sci. 2003 Jun;94(6):492-6
references.pmid: 12824872
references.citation: Oral Microbiol Immunol. 1996 Aug;11(4):266-73
references.pmid: 9002880
references.citation: Phytopathology. 2002 Jan;92(1):112-6
references.pmid: 18944147
references.citation: Infect Dis Clin North Am. 2001 Dec;15(4):1157-204
references.pmid: 11780271
references.citation: J Med Microbiol. 2002 May;51(5):443-447
references.pmid: 11990497
references.citation: J Clin Microbiol. 2001 Aug;39(8):2856-9
references.pmid: 11474003
references.citation: Pesqui Odontol Bras. 2003 Apr-Jun;17(2):142-6
references.pmid: 14569356
references.citation: Appl Environ Microbiol. 1997 Aug;63(8):3233-41
references.pmid: 9251210
references.citation: J Microbiol Methods. 2002 Apr;49(2):147-56
references.pmid: 11830300
references.citation: J Clin Microbiol. 1995 Jan;33(1):225-8
references.pmid: 7699047
references.citation: BMC Infect Dis. 2002 Nov 25;2:28
references.pmid: 12445330
references.citation: J Calif Dent Assoc. 1999 Oct;27(10):773-80, 782-4
references.pmid: 10726551
references.citation: J Endod. 2000 Dec;26(12):695-8
references.pmid: 11471635
references.citation: J Natl Cancer Inst. 2000 May 3;92(9):709-20
references.pmid: 10793107
references.citation: Cancer Res. 1996 Jun 1;56(11):2488-92
references.pmid: 8653682
references.citation: J Clin Microbiol. 1998 Jul;36(7):2068-72
references.pmid: 9650964
references.citation: Eur J Oral Sci. 1999 Apr;107(2):75-81
references.pmid: 10232454
references.citation: J Clin Periodontol. 2000 Oct;27(10):763-8
references.pmid: 11034125
references.citation: Caries Res. 2003 May-Jun;37(3):212-7
references.pmid: 12740546
references.citation: J Clin Microbiol. 2003 Sep;41(9):4438-41
references.pmid: 12958287
references.citation: J Clin Microbiol. 1997 Jun;35(6):1630-2
references.pmid: 9163503
references.citation: Appl Environ Microbiol. 2001 Oct;67(10):4479-87
references.pmid: 11571146
references.citation: J Periodontol. 2002 Jan;73(1):20-6
references.pmid: 11846195
references.citation: Clin Biochem Rev. 2006 Feb;27(1):39-51
references.pmid: 16886046
references.citation: Mol Cell Probes. 2001 Apr;15(2):71-3
references.pmid: 11292323
references.citation: FEMS Immunol Med Microbiol. 2002 Dec 13;34(4):277-81
references.pmid: 12443827
references.citation: J Dent. 2003 Jul;31(5):333-9
references.pmid: 12799118
references.citation: J Med Microbiol. 2000 Jan;49(1):63-71
references.pmid: 10628827
references.citation: Anal Biochem. 1997 Feb 15;245(2):154-60
references.pmid: 9056205
references.citation: Microbiol Immunol. 1999;43(5):485-90
references.pmid: 10449255
references.citation: Curr Opin Biotechnol. 2002 Jun;13(3):213-7
references.pmid: 12180095
references.citation: Mol Plant Pathol. 2001 Jan 1;2(1):49-57
references.pmid: 20572991
references.citation: J Oral Maxillofac Surg. 2002 Jul;60(7):808-15
references.pmid: 12089698
references.citation: Arch Pathol Lab Med. 2003 Sep;127(9):1112-20
references.pmid: 12946235
references.citation: Biopolymers. 1998 Jul;46(1):39-51
references.pmid: 9612138
references.citation: Lab Invest. 2001 Jun;81(6):905-12
references.pmid: 11406651
references.citation: Methods. 2001 Dec;25(4):419-29
references.pmid: 11846611
references.citation: J Periodontal Res. 2003 Oct;38(5):518-24
references.pmid: 12941077
references.citation: Arch Oral Biol. 1996 Aug-Sep;41(8-9):821-6
references.pmid: 9022919
references.citation: J Microbiol. 2005 Apr;43(2):204-8
references.pmid: 15880098
references.citation: Genome Res. 1996 Oct;6(10):995-1001
references.pmid: 8908519
references.citation: J Periodontol. 2001 Oct;72(10):1354-63
references.pmid: 11699477
references.citation: Microbiol Immunol. 2001;45(1):17-22
references.pmid: 11270602
references.citation: Res Microbiol. 2001 Mar;152(2):157-65
references.pmid: 11316369
references.citation: Pest Manag Sci. 2003 Feb;59(2):129-42
references.pmid: 12587866
references.citation: J Clin Microbiol. 2000 Feb;38(2):795-9
references.pmid: 10655387
references.citation: Intern Med J. 2003 Dec;33(12):613-5
references.pmid: 14656238
references.citation: Int J Cancer. 2001 Jul 15;93(2):162-71
references.pmid: 11410861
references.citation: J Antimicrob Chemother. 1996 Jan;37(1):187-8
references.pmid: 8647762
references.citation: Oral Microbiol Immunol. 1986 Nov;1(1):48-57
references.pmid: 3295680
references.citation: Lett Appl Microbiol. 2001 Jun;32(6):394-7
references.pmid: 11412350
references.citation: J Periodontol. 2002 Dec;73(12):1437-43
references.pmid: 12546093
references.citation: Clin Chem. 2000 Feb;46(2):147-8
references.pmid: 10657368
references.citation: J Microbiol Methods. 2001 Aug;46(2):99-105
references.pmid: 11412920
references.citation: Mol Aspects Med. 2006 Apr-Jun;27(2-3):95-125
references.pmid: 16460794
references.citation: J Med Microbiol. 2004 Oct;53(Pt 10):1029-1035
references.pmid: 15358827
references.citation: Anaerobe. 2005 Feb-Apr;11(1-2):79-91
references.pmid: 16701536
references.citation: Lancet Infect Dis. 2004 Jun;4(6):337-48
references.pmid: 15172342
references.citation: J Clin Microbiol. 1996 Dec;34(12):3016-22
references.pmid: 8940441
references.citation: Cancer Res. 2000 Dec 15;60(24):6878-81
references.pmid: 11156384
references.citation: Clin Cancer Res. 2002 May;8(5):1203-9
references.pmid: 12006539
references.citation: Int J Cancer. 1998 Nov 23;78(5):661-6
references.pmid: 9808539
references.citation: Appl Environ Microbiol. 2004 Jun;70(6):3575-81
references.pmid: 15184159
references.citation: Int J Cancer. 2000 Sep 20;89(5):411-7
references.pmid: 11008202
references.citation: Nucleic Acids Res. 2002 Mar 15;30(6):1292-305
references.pmid: 11884626
references.citation: J Clin Microbiol. 2004 Jan;42(1):198-202
references.pmid: 14715753
references.citation: Oncogene. 2002 Sep 19;21(42):6480-7
references.pmid: 12226751
references.citation: Cancer Res. 2001 Jul 15;61(14):5340-4
references.pmid: 11454671
references.citation: Oral Microbiol Immunol. 1996 Oct;11(5):289-93
references.pmid: 9028252
references.citation: Appl Environ Microbiol. 2002 Apr;68(4):1846-53
references.pmid: 11916704
references.citation: Ann Biol Clin (Paris). 1990;48(8):579-82
references.pmid: 2288446
references.citation: Nucleic Acids Res. 1997 Jun 15;25(12):2516-21
references.pmid: 9171107