title: RNA purification by preparative polyacrylamide gel electrophoresis.
abstract: Preparative polyacrylamide gel electrophoresis (PAGE) is a powerful tool for purifying RNA samples. Denaturing PAGE allows separation of nucleic acids that differ by a single nucleotide in length. It is commonly used to separate and purify RNA species after in vitro transcription, to purify naturally occurring RNA variants such as tRNAs, to remove degradation products, and to purify labeled RNA species. To preserve RNA integrity following purification, RNA is usually visualized by UV shadowing or stained with ethidium bromide or SYBR green dyes.
authors.affiliation: Stanford University School of Medicine, Stanford, CA, USA.
authors.forename: Alexey
authors.initials: A
authors.lastname: Petrov
authors.forename: Tinghe
authors.initials: T
authors.lastname: Wu
authors.forename: Elisabetta Viani
authors.initials: EV
authors.lastname: Puglisi
authors.forename: Joseph D
authors.initials: JD
authors.lastname: Puglisi
mesh_terms: D052160:Benzothiazoles; D003959:Diamines; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004996:Ethidium / Q000032:analysis; D005456:Fluorescent Dyes / Q000032:analysis; D009930:Organic Chemicals / Q000032:analysis; D011804:Quinolines; D012313:RNA / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D012313:RNA; D004996:Ethidium
keywords: Crush and soak' method; Electroelution; Gel preparation; Polyacrylamide gel electrophoresis (PAGE); RNA purification; RNA visualization