title: Chimeric newcastle disease virus protects chickens against avian influenza in the presence of maternally derived NDV immunity.
abstract: Newcastle disease virus (NDV), an avian paramyxovirus type 1, is a promising vector for expression of heterologous proteins from a variety of unrelated viruses including highly pathogenic avian influenza virus (HPAIV). However, pre-existing NDV antibodies may impair vector virus replication, resulting in an inefficient immune response against the foreign antigen. A chimeric NDV-based vector with functional surface glycoproteins unrelated to NDV could overcome this problem. Therefore, an NDV vector was constructed which carries the fusion (F) and hemagglutinin-neuraminidase (HN) proteins of avian paramyxovirus type 8 (APMV-8) instead of the corresponding NDV proteins in an NDV backbone derived from the lentogenic NDV Clone 30 and a gene expressing HPAIV H5 inserted between the F and HN genes. After successful virus rescue by reverse genetics, the resulting chNDVFHN PMV8H5 was characterized in vitro and in vivo. Expression and virion incorporation of the heterologous proteins was verified by Western blot and electron microscopy. Replication of the newly generated recombinant virus was comparable to parental NDV in embryonated chicken eggs. Immunization with chNDVFHN PMV8H5 stimulated full protection against lethal HPAIV infection in chickens without as well as with maternally derived NDV antibodies. Thus, tailored NDV vector vaccines can be provided for use in the presence or absence of routine NDV vaccination.
authors.affiliation: Institute of Molecular Biology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany.
authors.forename: Constanze
authors.initials: C
authors.lastname: Steglich
authors.forename: Christian
authors.initials: C
authors.lastname: Grund
authors.forename: Kristina
authors.initials: K
authors.lastname: Ramp
authors.forename: Angele
authors.initials: A
authors.lastname: Breithaupt
authors.forename: Dirk
authors.initials: D
authors.lastname: Höper
authors.forename: Günther
authors.initials: G
authors.lastname: Keil
authors.forename: Jutta
authors.initials: J
authors.lastname: Veits
authors.forename: Mario
authors.initials: M
authors.lastname: Ziller
authors.forename: Harald
authors.initials: H
authors.lastname: Granzow
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Mettenleiter
authors.forename: Angela
authors.initials: A
authors.lastname: Römer-Oberdörfer
mesh_terms: D000818:Animals; D002645:Chickens; D007109:Immunity / Q000276:immunology*; D005585:Influenza in Birds / Q000276:immunology* / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D014765:Viral Vaccines
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