title: Development of a novel rhesus macaque model with an infectious R5 simian-human immunodeficiency virus encoding HIV-1 CRF08_BC env.
abstract: BACKGROUND
  The CRF08_BC strain is one of the most predominant circulating Human immunodeficiency virus type 1 (HIV-1) strains in the Chinese pandemic. A simian-human immunodeficiency virus (SHIV) encoding HIV-1 CRF08_BC env is highly desirable to evaluate candidate AIDS vaccines in non-human primates.
  
  
  METHODS
  SHIV-KBQJ-12, which carries the envelope glycoprotein from QJ001, an infectious molecular clone of HIV-1 CRF08_BC, was generated. The replication capacity of SHIV-KBQJ-12 was determined both in human and rhesus macaque (Macaca mulatta) peripheral blood mononuclear cells (PBMCs) and in Chinese rhesus macaques.
  
  
  RESULTS
  SHIV-KBQJ-12 replicated efficiently in human and macaque PBMCs and displayed a preference for CCR5 as an entry coreceptor. Productive infection of two macaques by intravenous inoculation with SHIV-KBQJ-12 was confirmed.
  
  
  CONCLUSIONS
  SHIV-KBQJ-12 is an R5-tropic chimeric virus that can establish productive infection both in vitro and in vivo in Chinese rhesus macaques and will be useful to assess candidate HIV-1 CRF08_BC vaccines in China.
authors.affiliation: Research Group of HIV Molecular Epidemiology and Virology, Center for Emerging Infectious Diseases, The State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kusagawa
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zeng
authors.forename: Guibo
authors.initials: G
authors.lastname: Yang
authors.forename: Binlian
authors.initials: B
authors.lastname: Sun
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Miura
authors.forename: Rongge
authors.initials: R
authors.lastname: Yang
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000818:Animals; D018791:CD4 Lymphocyte Count; D002681:China; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D016679:Genome, Viral; D015658:HIV Infections / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000821:virology; D008253:Macaca mulatta; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D019713:Receptors, CCR5; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D019562:Viral Load; D014779:Virus Replication; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics* / Q000378:metabolism
chemical_list: D016915:AIDS Vaccines; D019713:Receptors, CCR5; D054299:env Gene Products, Human Immunodeficiency Virus
keywords: CRF08_BC; Chinese rhesus macaques; R5; peripheral blood mononuclear cell; simian-human immunodeficiency virus