title: Sauchinone from Saururus chinensis protects vascular inflammation by heme oxygenase-1 induction in human umbilical vein endothelial cells.
abstract: Sauchinone, a diastereomeric lignan isolated from the roots of Saururus chinensis (LOUR.) BAILL. (Saururaceae), is reported to exert a variety of biological activities such as hepatoprotective, anti-inflammatory actions and inhibitory effects on bone resorption. In this study, we investigated the effect of sauchinone in suppressing cell adhesion molecules such as vascular cell adhesion molecule-1 (VCAM-1) and intracellular adhesion molecule-1 (ICAM-1) expression in high glucose stimulated human umbilical vein endothelial cells (HUVEC). Sauchinone inhibited the phosphorylation and degradation of IκB-α, as well as the nuclear translocation of nuclear factor kappa B (NF-κB) p65 caused by the stimulation of high glucose. In addition, sauchinone induced heme oxygenase (HO)-1 expression through nuclear translocation of nuclear factor E2-related factor 2 in HUVEC. The effects of sauchinone on the high glucose-induced expression of VCAM-1 and ICAM-1 and nuclear translocation of NF-κB p65 were partially reversed by transfection of the cells with HO-1 siRNA. These findings suggest that sauchinone-induced HO-1 expression plays a key role in the vascular protective effects of sauchinone in HUVEC.
authors.affiliation: Hanbang Body-fluid Research Center, Wonkwang University, Shinyong-dong, Iksan Jeonbuk, 570-749, Republic of Korea.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, Shinyong-dong, Iksan, Jeonbuk 570-749, Republic of Korea; Hanbang Body-fluid Research Center, Wonkwang University, Shinyong-dong, Iksan Jeonbuk, 570-749, Republic of Korea.
authors.forename: Yun Jung
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Standardized Material Bank for New Botanical Drugs; College of Pharmacy, Wonkwang University, Iksan 570-749, Republic of Korea.
authors.forename: Youn Chul
authors.initials: YC
authors.lastname: Kim
authors.affiliation: College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, Shinyong-dong, Iksan, Jeonbuk 570-749, Republic of Korea; Hanbang Body-fluid Research Center, Wonkwang University, Shinyong-dong, Iksan Jeonbuk, 570-749, Republic of Korea.
authors.forename: Jung Joo
authors.initials: JJ
authors.lastname: Yoon
authors.affiliation: College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, Shinyong-dong, Iksan, Jeonbuk 570-749, Republic of Korea; Hanbang Body-fluid Research Center, Wonkwang University, Shinyong-dong, Iksan Jeonbuk, 570-749, Republic of Korea.
authors.forename: So Min
authors.initials: SM
authors.lastname: Lee
authors.affiliation: College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, Shinyong-dong, Iksan, Jeonbuk 570-749, Republic of Korea; Hanbang Body-fluid Research Center, Wonkwang University, Shinyong-dong, Iksan Jeonbuk, 570-749, Republic of Korea.
authors.forename: Yong Pyo
authors.initials: YP
authors.lastname: Lee
authors.affiliation: College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, Shinyong-dong, Iksan, Jeonbuk 570-749, Republic of Korea; Hanbang Body-fluid Research Center, Wonkwang University, Shinyong-dong, Iksan Jeonbuk, 570-749, Republic of Korea. Electronic address: dgkang@wku.ac.kr.
authors.forename: Dae Gill
authors.initials: DG
authors.lastname: Kang
authors.affiliation: College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, Shinyong-dong, Iksan, Jeonbuk 570-749, Republic of Korea; Hanbang Body-fluid Research Center, Wonkwang University, Shinyong-dong, Iksan Jeonbuk, 570-749, Republic of Korea. Electronic address: host@wku.ac.kr.
authors.forename: Ho Sub
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001578:Benzopyrans / Q000494:pharmacology / Q000627:therapeutic use*; D004149:Dioxoles / Q000494:pharmacology / Q000627:therapeutic use*; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects; D006801:Humans; D051550:I-kappa B Kinase / Q000378:metabolism; D020672:I-kappa B Proteins / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism / Q000517:prevention & control*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D010766:Phosphorylation; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D032134:Saururaceae / Q000737:chemistry*; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism; D014652:Vascular Diseases / Q000378:metabolism / Q000517:prevention & control*
chemical_list: D000893:Anti-Inflammatory Agents; D001578:Benzopyrans; D004149:Dioxoles; D020672:I-kappa B Proteins; D051267:NF-E2-Related Factor 2; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; C410958:sauchinone; D018799:Intercellular Adhesion Molecule-1; D000072000:NF-KappaB Inhibitor alpha; D051547:Heme Oxygenase-1; D051550:I-kappa B Kinase
keywords: Atherosclerosis; HUVEC; Heme oxygenase-1; NF-κB; Sauchinone