title: Microbial production of biovanillin.
abstract: This review aims at providing an overview on the microbial production of vanillin, a new alternative method for the production of this important flavor of the food industry, which has the potential to become economically competitive in the next future. After a brief description of the applications of vanillin in different industrial sectors and of its physicochemical properties, we described the traditional ways of providing vanillin, specifically extraction and chemical synthesis (mainly oxidation) and compared them with the new biotechnological options, i.e., biotransformations of caffeic acid, veratraldehyde and mainly ferulic acid. In the second part of the review, emphasis has been addressed to the factors most influencing the bioproduction of vanillin, specifically the age of inoculum, pH, temperature, type of co-substrate, as well as the inhibitory effects exerted either by excess substrate or product. The final part of the work summarized the downstream processes and the related unit operations involved in the recovery of vanillin from the bioconversion medium.
authors.affiliation: Department of Chemical and Process Engineering, University of Genoa , Via Opera Pia 15, 16145 Genoa , Italy.
authors.forename: A
authors.initials: A
authors.lastname: Converti
authors.forename: B
authors.initials: B
authors.lastname: Aliakbarian
authors.forename: J M
authors.initials: JM
authors.lastname: Domínguez
authors.forename: G
authors.initials: G
authors.lastname: Bustos Vázquez
authors.forename: P
authors.initials: P
authors.lastname: Perego
keywords: Escherichia coli; bioconversion; ferulic acid; review; vanillin
references.citation: J Ind Microbiol. 1995 Dec;15(6):457-71
references.pmid: 8821508
references.citation: Annu Rev Microbiol. 1994;48:773-800
references.pmid: 7826026
references.citation: Phytochemistry. 2003 Jul;63(5):505-15
references.pmid: 12809710
references.citation: Appl Environ Microbiol. 1999 Nov;65(11):4837-47
references.pmid: 10543794
references.citation: Appl Microbiol Biotechnol. 2001 Aug;56(3-4):296-314
references.pmid: 11548997
references.citation: Extremophiles. 1998 Aug;2(3):249-56
references.pmid: 9783172
references.citation: Appl Biochem Biotechnol. 2002 Jul-Dec;102-103(1-6):141-53
references.pmid: 12396118
references.citation: Can J Microbiol. 1983 Oct;29(10):1253-7
references.pmid: 6661696
references.citation: Crit Rev Biotechnol. 1995;15(2):139-77
references.pmid: 7641292
references.citation: Microbiology (Reading). 1998 Aug;144 ( Pt 8):2011-2023
references.pmid: 9720023
references.citation: Appl Microbiol Biotechnol. 2004 Jun;64(5):644-50
references.pmid: 14730410
references.citation: Trends Biotechnol. 2005 Apr;23(4):193-8
references.pmid: 15780711
references.citation: Curr Opin Biotechnol. 2000 Oct;11(5):490-6
references.pmid: 11024369
references.citation: Appl Microbiol Biotechnol. 1998 Jan;49(1):1-8
references.pmid: 9487704
references.citation: Enzyme Microb Technol. 2001 Jan 2;28(1):70-80
references.pmid: 11118600
references.citation: Appl Environ Microbiol. 1997 Feb;63(2):370-9
references.pmid: 9023917
references.citation: Microbiology (Reading). 1998 May;144 ( Pt 5):1397-1405
references.pmid: 9611814
references.citation: Extremophiles. 1998 Aug;2(3):239-48
references.pmid: 9783171
references.citation: Microb Cell Fact. 2007 Apr 16;6:13
references.pmid: 17437627
references.citation: Trends Biotechnol. 1999 Jul;17(7):282-9
references.pmid: 10370235
references.citation: J Biotechnol. 2006 Sep 18;125(3):369-76
references.pmid: 16677732
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jul 25;807(1):3-11
references.pmid: 15177153
references.citation: J Food Prot. 1996 Dec;59(13):82-86
references.pmid: 28384019
references.citation: Biotechnol Bioeng. 1986 May;28(5):711-7
references.pmid: 18555382
references.citation: J Agric Food Chem. 2001 Jun;49(6):2954-8
references.pmid: 11409992
references.citation: Appl Environ Microbiol. 2003 Nov;69(11):6569-76
references.pmid: 14602615
references.citation: Plant Cell Rep. 2004 Jul;22(12):959-66
references.pmid: 15118832
references.citation: Biotechnol Lett. 2005 Nov;27(22):1829-32
references.pmid: 16314978
references.citation: Plant Physiol Biochem. 2005 Feb;43(2):125-31
references.pmid: 15820659
references.citation: Bioresour Technol. 2007 Mar;98(5):1115-9
references.pmid: 16782330
references.citation: Biotechnol Bioeng. 1983 Aug;25(8):2007-23
references.pmid: 18551547