title: ITIH4: A New Potential Biomarker of "Toxin Syndrome" in Coronary Heart Disease Patient Identified with Proteomic Method.
abstract: Objective. This trial aims to look for the protein biomarker of "toxin syndrome" of CHD patients. Methods. We have performed two trials in this paper. The first trial was a randomized controlled trial (RCT) of the plasma proteome in unstable angina (UA) patients by Maldi-Tof Mass. The second trial was a nested case-control study in 1503 stable CHD patients with one-year followup for acute cardiovascular events (ACEs). Results. In the RCT study, 12 protein spots were found to be the differential protein for the significant differences between the difference of before and after treatment in group A and group B; 2 of them (3207.37 Da and 4279.95 Da) was considered to be unique to "toxin syndrome" for being differential proteins of group B but not group A. These 2 spots were identified as Isoform 1 of Fibrinogen alpha chain precursor (FGA, 3207.37 Da) and Isoform 2 of inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4, 4279.95 Da), respectively. In the nested case-control study, the result of Western blot demonstrated that protein expression of ITIH4 in the group with followup ACEs was significantly lower than the matched group without followup ACEs (P = 0.027). Conclusion. ITIH4 might be a new potential biomarker of CHD "toxin syndrome" in TCM, indicating the potential role in early identifying high-risk CHD patients in stable period.
authors.affiliation: Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Shang
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Jianpeng
authors.initials: J
authors.lastname: Du
authors.forename: Jianyan
authors.initials: J
authors.lastname: Wen
authors.forename: Geng
authors.initials: G
authors.lastname: Li
authors.forename: Dazhuo
authors.initials: D
authors.lastname: Shi
authors.forename: Keji
authors.initials: K
authors.lastname: Chen
references.citation: Zhongguo Zhong Xi Yi Jie He Za Zhi. 2001 Aug;21(8):591-4
references.pmid: 12575573
references.citation: Clin Chim Acta. 2009 Apr;402(1-2):160-3
references.pmid: 19263524
references.citation: Zhongguo Zhong Xi Yi Jie He Za Zhi. 2008 Oct;28(10):934-8
references.pmid: 19123336
references.citation: Zhongguo Zhong Xi Yi Jie He Za Zhi. 2007 Jan;27(1):40-4
references.pmid: 17302063
references.citation: Inflamm Res. 2000 Jun;49(6):305-10
references.pmid: 10939621
references.citation: Chin J Integr Med. 2011 Sep;17(9):669-74
references.pmid: 21910067
references.citation: Zhongguo Zhong Yao Za Zhi. 2008 Sep;33(18):2013-6
references.pmid: 19160773
references.citation: Zhonghua Xin Xue Guan Bing Za Zhi. 2007 Apr;35(4):295-304
references.pmid: 17711651
references.citation: Zhongguo Zhong Xi Yi Jie He Za Zhi. 2008 Feb;28(2):126-30
references.pmid: 18386574
references.citation: Zhongguo Zhong Xi Yi Jie He Za Zhi. 2010 Feb;30(2):125-9
references.pmid: 20462035
references.citation: Zhongguo Zhong Xi Yi Jie He Za Zhi. 2012 May;32(5):603-6
references.pmid: 22679717
references.citation: Zhongguo Zhong Xi Yi Jie He Za Zhi. 2012 Sep;32(9):1285-6
references.pmid: 23185776
references.citation: Nihon Ronen Igakkai Zasshi. 2000 Jun;37(6):458-63
references.pmid: 10998926
references.citation: Zhongguo Zhong Xi Yi Jie He Za Zhi. 2008 Dec;28(12):1100-3
references.pmid: 19317167
references.citation: FEBS Lett. 2005 Jan 3;579(1):115-21
references.pmid: 15620699
references.citation: Chin J Integr Med. 2011 May;17(5):333-8
references.pmid: 21611895
references.citation: Circulation. 1979 Mar;59(3):607-9
references.pmid: 761341
references.citation: Infect Immun. 2004 Jul;72(7):3777-82
references.pmid: 15213118
references.citation: Zhong Xi Yi Jie He Xue Bao. 2010 Mar;8(3):238-43
references.pmid: 20226145
references.citation: Zhongguo Zhong Xi Yi Jie He Za Zhi. 2005 Apr;25(4):345-9
references.pmid: 15892282
references.citation: Coron Artery Dis. 2001 May;12(3):157-65
references.pmid: 11352071
references.citation: Chin J Integr Med. 2011 Nov;17(11):818-23
references.pmid: 22057410
references.citation: Chin Med J (Engl). 2006 Jan 5;119(1):6-13
references.pmid: 16454975
references.citation: Zhongguo Zhong Xi Yi Jie He Za Zhi. 2008 Oct;28(10):912-6
references.pmid: 19123330
references.citation: J Hum Genet. 2004;49(1):24-28
references.pmid: 14661079
references.citation: Chin J Integr Med. 2011 Nov;17(11):803-10
references.pmid: 22057408
references.citation: Zhongguo Zhong Xi Yi Jie He Za Zhi. 2010 Dec;30(12):1279-83
references.pmid: 21302491