title: Effects of di-n-butyl phthalate and diethyl phthalate on acetylcholinesterase activity and neurotoxicity related gene expression in embryonic zebrafish.
abstract: In the present study, zebrafish embryos were used to assess the neurotoxicity of di-n-butyl phthalate (DBP), diethyl phthalate (DEP) and their mixture. Four-hour post-fertilization (hpf) zebrafish embryos were exposed to various concentrations of DBP, DEP and their mixture (DBP-DEP) until 96 hpf. The transcriptions levels of selected neuron-related genes reported as neurotoxicity biomarkers were analyzed. The results showed that transcripts of growth associated protein 43 (gap43), embryonic lethal abnormal vision-like 3 (elavl3), glial fibrillary acidic protein (gfap), myelin basic protein (mbp), α1-tubulin and neurogenin1 (ngn1) were significantly up-regulated after DBP, DEP and DBP-DEP mixture exposure. In addition, acetylcholinesterase activity was significantly inhibited in the embryos. These results indicate that DBP and DEP have the potential neurotoxicity in zebrafish embryos.
authors.affiliation: School of the Environment, Jiangsu University, Xuefu Rd. 301, Zhenjiang, 212013, Jiangsu, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Xu
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Shao
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Zou
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.forename: Songlei
authors.initials: S
authors.lastname: Han
authors.forename: Shasha
authors.initials: S
authors.lastname: Wang
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Wu
authors.forename: Liuqing
authors.initials: L
authors.lastname: Yang
authors.forename: Zhonglin
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D003993:Dibutyl Phthalate / Q000633:toxicity*; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D009420:Nervous System / Q000187:drug effects* / Q000378:metabolism; D010795:Phthalic Acids / Q000633:toxicity*; D015027:Zebrafish / Q000196:embryology*
chemical_list: D010795:Phthalic Acids; D003993:Dibutyl Phthalate; D000110:Acetylcholinesterase; C007379:diethyl phthalate