title: Nanoencapsulation enhances epigallocatechin-3-gallate stability and its antiatherogenic bioactivities in macrophages.
abstract: We have successfully synthesized (-)-epigallocatechin-3-gallate (EGCG) encapsulated nanostructured lipid carriers (NLCE) and chitosan-coated NLCE (CSNLCE) using natural lipids, surfactant, chitosan, and EGCG. Nanoencapsulation dramatically improved EGCG stability. CSNLCE significantly increased EGCG content in THP-1-derived macrophages compared with nonencapsulated EGCG. As compared to 10 μM nonencapsulated EGCG, both NLCE and CSNLCE at the same concentration significantly decreased macrophage cholesteryl ester content. NLCE and CSNLCE significantly decreased mRNA levels and protein secretion of monocyte chemoattractant protein-1 (MCP-1) levels in macrophages, respectively. These data suggest that nanoencapsulated EGCG may have a potential to inhibit atherosclerotic lesion development through decreasing macrophage cholesterol content and MCP-1 expression.
authors.affiliation: Nutritional Science Program, Texas Tech University, Lubbock, TX, 79409.
authors.forename: Jia
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Nutritional Science Program, Texas Tech University, Lubbock, TX, 79409.
authors.forename: Shufang
authors.initials: S
authors.lastname: Nie
authors.affiliation: Nutritional Science Program, Texas Tech University, Lubbock, TX, 79409.
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
mesh_terms: D050197:Atherosclerosis / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D028241:Camellia sinensis / Q000737:chemistry*; D002317:Cardiovascular Agents / Q000737:chemistry* / Q000494:pharmacology; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D004339:Drug Compounding; D004355:Drug Stability; D006801:Humans; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology
chemical_list: C111835:CCL2 protein, human; D002317:Cardiovascular Agents; D018932:Chemokine CCL2; D010936:Plant Extracts; D002392:Catechin; D002784:Cholesterol; C045651:epigallocatechin gallate
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