title: Explanatory chapter: nuclease protection assays.
abstract: Nuclease protection assays are a highly sensitive, solution-based technique used to detect and quantify specific RNA targets from complex RNA mixtures. Today, this technique is frequently performed using kits, and the following chapter will explain the principles of how these kits work and some considerations to keep in mind when using them.
authors.affiliation: Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: ehuang11@jhmi.edu.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Eyler
mesh_terms: D000818:Animals; D006801:Humans; D023282:Nuclease Protection Assays / Q000379:methods*; D012313:RNA / Q000032:analysis / Q000378:metabolism; D015347:RNA Probes / Q000032:analysis / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism
chemical_list: D015347:RNA Probes; D012313:RNA; D012260:Ribonucleases
keywords: Electrophoretic separation and detection; Nuclease digestion; Nuclease inactivation and probe recovery; Nuclease protection assays; interpretation and troubleshooting