title: Quantification of plaque area and characterization of plaque biochemical composition with atherosclerosis progression in ApoE/LDLR(-/-) mice by FT-IR imaging.
abstract: In this work the quantitative determination of atherosclerotic lesion area (ApoE/LDLR(-/-) mice) by FT-IR imaging is presented and validated by comparison with atherosclerotic lesion area determination by classic Oil Red O staining. Cluster analysis of FT-IR-based measurements in the 2800-3025 cm(-1) range allowed for quantitative analysis of the atherosclerosis plaque area, the results of which were highly correlated with those of Oil Red O histological staining (R(2) = 0.935). Moreover, a specific class obtained from a second cluster analysis of the aortic cross-section samples at different stages of disease progression (3, 4 and 6 months old) seemed to represent the macrophages (CD68) area within the atherosclerotic plaque.
authors.affiliation: Faculty of Chemistry, Jagiellonian University, 3 Ingardena Str., 30-060 Krakow, Poland. baranska@chemia.uj.edu.pl.
authors.forename: Tomasz P
authors.initials: TP
authors.lastname: Wrobel
authors.forename: Lukasz
authors.initials: L
authors.lastname: Mateuszuk
authors.forename: Renata B
authors.initials: RB
authors.lastname: Kostogrys
authors.forename: Stefan
authors.initials: S
authors.lastname: Chlopicki
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Baranska
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency*; D050197:Atherosclerosis / Q000378:metabolism; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D058226:Plaque, Atherosclerotic / Q000737:chemistry* / Q000378:metabolism; D011973:Receptors, LDL / Q000172:deficiency*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D001057:Apolipoproteins E; D011973:Receptors, LDL