title: Quantitative full time course analysis of nonlinear enzyme cycling kinetics.
abstract: Enzyme inhibition due to the reversible binding of reaction products is common and underlies the origins of negative feedback inhibition in many metabolic and signaling pathways. Product inhibition generates non-linearity in steady-state time courses of enzyme activity, which limits the utility of well-established enzymology approaches developed under the assumption of irreversible product release. For more than a century, numerous attempts to find a mathematical solution for analysis of kinetic time courses with product inhibition have been put forth. However, no practical general method capable of extracting common enzymatic parameters from such non-linear time courses has been successfully developed. Here we present a simple and practical method of analysis capable of efficiently extracting steady-state enzyme kinetic parameters and product binding constants from non-linear kinetic time courses with product inhibition and/or substrate depletion. The method is general and applicable to all enzyme systems, independent of reaction schemes and pathways.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA.
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Cao
authors.forename: Enrique M
authors.initials: EM
authors.lastname: De La Cruz
mesh_terms: D000465:Algorithms; D003198:Computer Simulation; D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D008954:Models, Biological; D013379:Substrate Specificity
chemical_list: D004798:Enzymes
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