title: Biosurfactant production by rhodococcus erythropolis and its application to oil removal.
abstract: The influence of different nutrients on biosurfactant production by Rhodococcus erythropolis was investigated. Increasing the concentration of phosphate buffer from 30 up through 150 mmol/L stimulated an increase in biosurfactant production, which reached a maximum concentration of 285 mg/L in shaken flasks. Statistical analysis showed that glycerol, NaNO3, MgSO4 and yeast extract had significant effects on production. The results were confirmed in a batchwise bioreactor, and semi-growth-associated production was detected. Reduction in the surface tension, which indicates the presence of biosurfactant, reached a value of 38 mN/m at the end of 35 hours. Use of the produced biosurfactant for washing crude oil-contaminated soil showed that 2 and 4 times the critical micellar concentration (CMC) were able to remove 97 and 99% of the oil, respectively, after 1 month of impregnation.
authors.affiliation: Laboratúrios de Desenvolvimento de Bioprocessos, Departamento de Engenharia Bioquímica, Universidade Federal do Rio de Janeiro, Faculdade de Química, Centro de Tecnologia , Rio de Janeiro, RJ , Brasil.
authors.forename: Graziela Jardim
authors.initials: GJ
authors.lastname: Pacheco
authors.forename: Elisa Mara Prioli
authors.initials: EM
authors.lastname: Ciapina
authors.forename: Edelvio de Barros
authors.initials: Ede B
authors.lastname: Gomes
authors.forename: Nei Pereira
authors.initials: NP
authors.lastname: Junior
keywords: Biosurfactant production; Rhodococcus erythropolis; oil removal
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