title: Inhibitory effects of tannic acid on fatty acid synthase and 3T3-L1 preadipocyte.
abstract: Tannic acid is a hydrolyzable tannin that exists in many widespread edible plants with a variety of biological activities. In this study, we found that tannic acid potently inhibited the activity of fatty acid synthase (FAS) in a concentration-dependent manner with a half-inhibitory concentration value (IC50) of 0.14 microM. The inhibition kinetic results showed that the inhibition of FAS by tannic acid was mixed competitive and noncompetitive manner with respect to acetyl-CoA and malonyl-CoA, but uncompetitive to NADPH. Tannic acid prevented the differentiation of 3T3-L1 pre-adipocytes, and thus repressed intracellular lipid accumulation. In the meantime, tannic acid decreased the expression of FAS and down-regulated the mRNA level of FAS and PPARgamma during adipocyte differentiation. Further studies showed that the inhibitory effect of tannic acid did not relate to FAS non-specific sedimentation. Since FAS was believed to be a therapeutic target of obesity, these findings suggested that tannic acid was considered having potential in the prevention of obesity.
authors.affiliation: College of Life Sciences, University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, China.
authors.forename: Huijin
authors.initials: H
authors.lastname: Fan
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.forename: Weixi
authors.initials: W
authors.lastname: Tian
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ma
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D015536:Down-Regulation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D064429:Fatty Acid Synthases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects; D051379:Mice; D009765:Obesity / Q000188:drug therapy / Q000201:enzymology; D047495:PPAR gamma / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D013634:Tannins / Q000494:pharmacology*
chemical_list: D004791:Enzyme Inhibitors; D047495:PPAR gamma; D012333:RNA, Messenger; D013634:Tannins; D064429:Fatty Acid Synthases