title: Problem-solving test: conditional gene targeting using the Cre/loxP recombination system.
abstract: Terms to be familiar with before you start to solve the test: gene targeting, knock-out mutation, bacteriophage, complementary base-pairing, homologous recombination, deletion, transgenic organisms, promoter, polyadenylation element, transgene, DNA replication, RNA polymerase, Shine-Dalgarno sequence, restriction endonuclease, polymerase chain reaction, primer, transcription, SV40 virus, malignant transformation, spacer region, gene expression, agarose gel electrophoresis, ethidium bromide staining, Southern-blotting, probe, reporter gene.
authors.forename: József
authors.initials: J
authors.lastname: Szeberényi
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001671:Biochemistry / Q000193:education / Q000379:methods; D015139:Blotting, Southern; D004587:Electrophoresis, Agar Gel; D055786:Gene Knockout Techniques; D018390:Gene Targeting / Q000379:methods*; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D008967:Molecular Biology / Q000193:education / Q000379:methods; D016133:Polymerase Chain Reaction; D011340:Problem Solving; D011995:Recombination, Genetic; D011795:Surveys and Questionnaires; D013663:Teaching / Q000379:methods; D009626:Terminology as Topic