title: Isolation and characterization of an Ashbya gossypii mutant for improved riboflavin production.
abstract: The use of the filamentous fungus, Ashbya gossypii, to improve riboflavin production at an industrial scale is described in this paper. A riboflavin overproducing strain was isolated by ultraviolet irradiation. Ten minutes after spore suspensions of A. gossypii were irradiated by ultraviolet light, a survival rate of 5.5% spores was observed, with 10% of the surviving spores giving rise to riboflavin-overproducing mutants. At this time point, a stable mutant of the wild strain was isolated. Riboflavin production of the mutant was two fold higher than that of the wild strain in flask culture. When the mutant was growing on the optimized medium, maximum riboflavin production could reach 6.38 g/l. It has even greater promise to increase its riboflavin production through dynamic analysis of its growth phase parameters, and riboflavin production could reach 8.12 g/l with pH was adjusted to the range of 6.0-7.0 using KH2PO4 in the later growth phase. This mutant has the potential to be used for industrial scale riboflavin production.
authors.affiliation: School of Marine Sciences, China University of Geosciences , 29 Xueyuan Rd., Beijing 100083 , China.
authors.forename: Shiping
authors.initials: S
authors.lastname: Wei
authors.forename: James
authors.initials: J
authors.lastname: Hurley
authors.forename: Zhenglong
authors.initials: Z
authors.lastname: Jiang
authors.forename: Siwen
authors.initials: S
authors.lastname: Wang
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
keywords: Ashbya gossypii; Medium optimization; Riboflavin; Ultraviolet irradiation
references.citation: J Appl Microbiol. 2007 Aug;103(2):468-76
references.pmid: 17650208
references.citation: Braz J Microbiol. 2010 Jan;41(1):186-95
references.pmid: 24031480
references.citation: J Bacteriol. 1949 Dec;58(6):737-45
references.pmid: 15395174
references.citation: J Biosci Bioeng. 2004;97(1):59-64
references.pmid: 16233590
references.citation: Appl Microbiol Biotechnol. 2000 May;53(5):509-16
references.pmid: 10855708
references.citation: J Biosci Bioeng. 2009 Oct;108(4):325-9
references.pmid: 19716523
references.citation: J Chem Technol Biotechnol. 1992;53(4):313-27
references.pmid: 1368195
references.citation: Appl Microbiol Biotechnol. 2002 Mar;58(3):275-85
references.pmid: 11935176
references.citation: Microbiology (Reading). 1996 Feb;142(2):411-417
references.pmid: 33657743
references.citation: Annu Rev Microbiol. 1972;26:369-88
references.pmid: 4567521
references.citation: Appl Environ Microbiol. 2005 Oct;71(10):5743-51
references.pmid: 16204483
references.citation: Appl Microbiol Biotechnol. 2009 Jun;83(3):529-39
references.pmid: 19343342
references.citation: Environ Microbiol. 2001 Sep;3(9):545-50
references.pmid: 11683864
references.citation: J Ind Microbiol Biotechnol. 2010 Jan;37(1):57-64
references.pmid: 19826846