title: A nanopore machine promotes the vectorial transport of DNA across membranes.
abstract: The transport of nucleic acids through membrane pores is a fundamental biological process that occurs in all living organisms. It occurs, for example, during the import of viral DNA into the host cell or during the nuclear pore complex-mediated transport of mRNA in and out the cell nucleus and has implications in nucleic acid drug delivery and gene therapy. Here we describe an engineered DNA transporter that is able to recognize and chaperone a specific DNA molecule across a biological membrane under a fixed transmembrane potential. The transported DNA strand is then released by a simple mechanism based on DNA strand displacement. This nanopore machine might be used to separate or concentrate nucleic acids or to transport genetic information across biological membranes.
authors.affiliation: Department of Chemistry, University of Leuven, Leuven 3001, Belgium.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Franceschini
authors.forename: Misha
authors.initials: M
authors.lastname: Soskine
authors.forename: Annemie
authors.initials: A
authors.lastname: Biesemans
authors.forename: Giovanni
authors.initials: G
authors.lastname: Maglia
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D007700:Kinetics; D058608:Nanopores; D043862:Rotaxanes / Q000378:metabolism; D013816:Thermodynamics
chemical_list: D001426:Bacterial Proteins; D043862:Rotaxanes; D004247:DNA
references.citation: Phys Rev Lett. 2001 Apr 9;86(15):3435-8
references.pmid: 11327989
references.citation: Nat Biotechnol. 2000 Oct;18(10):1091-5
references.pmid: 11017049
references.citation: Nature. 2002 Jan 3;415(6867):62-5
references.pmid: 11780115
references.citation: Nano Lett. 2012 Sep 12;12(9):4895-900
references.pmid: 22849517
references.citation: Nano Lett. 2009 Nov;9(11):3831-6
references.pmid: 19645477
references.citation: Science. 2013 May 3;340(6132):595-9
references.pmid: 23579496
references.citation: Nano Lett. 2012 Apr 11;12(4):2117-22
references.pmid: 22401838
references.citation: Nat Nanotechnol. 2010 Feb;5(2):160-5
references.pmid: 20023645
references.citation: Biophys J. 2004 Apr;86(4):2530-7
references.pmid: 15041689
references.citation: Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6185-90
references.pmid: 9177192
references.citation: Science. 2011 Jun 3;332(6034):1196-201
references.pmid: 21636773
references.citation: Curr Opin Biotechnol. 2012 Aug;23(4):516-21
references.pmid: 22237015
references.citation: Angew Chem Int Ed Engl. 2000 Oct 2;39(19):3348-3391
references.pmid: 11091368
references.citation: Nat Nanotechnol. 2007 Aug;2(8):490-4
references.pmid: 18654346
references.citation: PLoS One. 2012;7(9):e46014
references.pmid: 23029365
references.citation: Chem Commun (Camb). 2012 Feb 1;48(10):1520-2
references.pmid: 22089628
references.citation: Angew Chem Int Ed Engl. 2012 May 14;51(20):4864-7
references.pmid: 22489067
references.citation: Nano Lett. 2005 Oct;5(10):1883-8
references.pmid: 16218703
references.citation: Nat Chem. 2011 Feb;3(2):103-13
references.pmid: 21258382
references.citation: Methods Enzymol. 2011;498:399-406
references.pmid: 21601687
references.citation: Nat Nanotechnol. 2010 Jun;5(6):436-42
references.pmid: 20400967
references.citation: Angew Chem Int Ed Engl. 2004 Sep 20;43(37):4906-11
references.pmid: 15372637
references.citation: Nat Nanotechnol. 2011 Jul 31;6(9):558-62
references.pmid: 21804552
references.citation: Nano Lett. 2013 Jun 12;13(6):2500-5
references.pmid: 23678965
references.citation: Acc Chem Res. 2001 Jun;34(6):433-44
references.pmid: 11412080
references.citation: Nature. 2010 May 13;465(7295):206-10
references.pmid: 20463735
references.citation: Nat Nanotechnol. 2011 Mar;6(3):166-9
references.pmid: 21297627
references.citation: Nature. 1991 Apr 18;350(6319):631-3
references.pmid: 2017259
references.citation: Science. 2004 Jul 2;305(5680):86-90
references.pmid: 15232106
references.citation: Science. 2012 Nov 16;338(6109):932-6
references.pmid: 23161995
references.citation: Nature. 2008 Jan 17;451(7176):318-22
references.pmid: 18202654
references.citation: Science. 2012 Nov 30;338(6111):1177-83
references.pmid: 23197527
references.citation: Nano Lett. 2012 Jan 11;12(1):512-7
references.pmid: 22196850
references.citation: J Am Chem Soc. 2009 Dec 2;131(47):17303-14
references.pmid: 19894722
references.citation: Nature. 2009 Jun 4;459(7247):726-30
references.pmid: 19421192
references.citation: Nano Lett. 2013 Jun 12;13(6):2351-6
references.pmid: 23611515
references.citation: Nat Chem Biol. 2013 Jun;9(6):362-6
references.pmid: 23624438
references.citation: Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19720-5
references.pmid: 19060213
references.citation: Methods Enzymol. 2010;475:591-623
references.pmid: 20627172
references.citation: Phys Rev Lett. 2000 Oct 2;85(14):3057-60
references.pmid: 11006002
references.citation: Biophys J. 2001 Aug;81(2):1133-43
references.pmid: 11463654
references.citation: Nature. 2009 May 21;459(7245):414-8
references.pmid: 19458720
references.citation: Science. 2012 Jun 1;336(6085):1171-4
references.pmid: 22654060
references.citation: Angew Chem Int Ed Engl. 2004 Jun 7;43(23):3063-7
references.pmid: 15188482
references.citation: J Am Chem Soc. 2004 Sep 8;126(35):10834-5
references.pmid: 15339155
references.citation: Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20647-52
references.pmid: 19098105
references.citation: Nano Lett. 2010 Apr 14;10(4):1414-20
references.pmid: 20235508