title: Detection of Mycoplasma hyopneumoniae by polymerase chain reaction in swine presenting respiratory problems.
abstract: Since Mycoplasma hyopneumoniae isolation in appropriate media is a difficult task and impractical for daily routine diagnostics, Nested-PCR (N-PCR) techniques are currently used to improve the direct diagnostic sensitivity of Swine Enzootic Pneumonia. In a first experiment, this paper describes a N-PCR technique optimization based on three variables: different sampling sites, sample transport media, and DNA extraction methods, using eight pigs. Based on the optimization results, a second experiment was conducted for testing validity using 40 animals. In conclusion, the obtained results of the N-PCR optimization and validation allow us to recommend this test as a routine monitoring diagnostic method for Mycoplasma hyopneumoniae infection in swine herds.
authors.affiliation: Universidade Estadual de Londrina, Campus Universitário , Londrina, PR , Brasil.
authors.forename: M
authors.initials: M
authors.lastname: Yamaguti
authors.forename: E E
authors.initials: EE
authors.lastname: Muller
authors.forename: A I
authors.initials: AI
authors.lastname: Piffer
authors.forename: J D
authors.initials: JD
authors.lastname: Kich
authors.forename: C S
authors.initials: CS
authors.lastname: Klein
authors.forename: S S
authors.initials: SS
authors.lastname: Kuchiishi
keywords: Mycoplasma hyopneumoniae; Nested- PCR; Swine Enzootic Pneumonia; diagnosis
references.citation: J Clin Microbiol. 1998 Jul;36(7):1984-8
references.pmid: 9650949
references.citation: Vet Microbiol. 2000 Sep 15;76(1):31-40
references.pmid: 10925039
references.citation: Acta Vet Scand. 1971;12(1):69-79
references.pmid: 5575144
references.citation: Vet Microbiol. 2006 Jun 15;115(1-3):111-6
references.pmid: 16540266
references.citation: J Clin Microbiol. 1999 Mar;37(3):620-7
references.pmid: 9986823
references.citation: Avian Dis. 1995 Oct-Dec;39(4):729-35
references.pmid: 8719206
references.citation: Mol Cell Probes. 1996 Feb;10(1):15-22
references.pmid: 8684372
references.citation: Lett Appl Microbiol. 1997 Nov;25(5):327-30
references.pmid: 9418066
references.citation: J Clin Microbiol. 1995 Apr;33(4):893-7
references.pmid: 7540629
references.citation: Lett Appl Microbiol. 1999 Oct;29(4):269-72
references.pmid: 10583757
references.citation: J Clin Microbiol. 1990 Mar;28(3):495-503
references.pmid: 1691208
references.citation: J Clin Microbiol. 1992 Aug;30(8):2025-8
references.pmid: 1500509
references.citation: Microbiol Rev. 1985 Dec;49(4):419-55
references.pmid: 3912655
references.citation: Clin Chem. 1997 Nov;43(11):2021-38
references.pmid: 9365385
references.citation: J Vet Med Sci. 2005 Aug;67(8):801-5
references.pmid: 16141667
references.citation: Vet Q. 1996 Sep;18(3):104-9
references.pmid: 8903144
references.citation: J Vet Diagn Invest. 1999 May;11(3):246-51
references.pmid: 10353356
references.citation: J Vet Diagn Invest. 2002 Nov;14(6):463-9
references.pmid: 12423027