title: Candida albicans: genotyping methods and clade related phenotypic characteristics.
abstract: Several molecular methods, such as Southern blotting hybridization, Multilocus Sequence Typing, and DNA microsatellite analysis, have been employed to genotype Candida albicans. The genotype analysis allows to group strains in clades, that is, a group composed of one ancestor and its descendants. These genotype studies demonstrate that clades distribution is influenced by geographic area as well as that antifungal resistance is associated with particular clades. These findings suggested that C. albicans reproduces mainly in a clonal manner, with certain degree of DNA microevolution. Additionally, virulence factors and site of isolation have also been associated with clade specificity. The present article is a brief review about the methods used for Candida genotyping and the correlated clade systems established. Special emphasis is given to Ca3 hybridization, MLST, and Microsatellites. The present work is also focused on the phenotypic and physiological traits associated with Candida clades.
authors.affiliation: Universidade Federal de São João Del Rei , São João Del-Rei, MG , Brasil ; Universidade do Vale do Paraíba , São José dos Campos, SP , Brasil.
authors.forename: Juliana P
authors.initials: JP
authors.lastname: Lyon
authors.forename: Karen C M
authors.initials: KC
authors.lastname: Moraes
authors.forename: Leonardo M
authors.initials: LM
authors.lastname: Moreira
authors.forename: Flávio
authors.initials: F
authors.lastname: Aimbire
authors.forename: Maria Aparecida
authors.initials: MA
authors.lastname: de Resende
keywords: Candida albicans; clonal reproduction; genotyping systems; microevolution; phenotypic characteristics
references.citation: Mycoses. 2000;43(3-4):109-17
references.pmid: 10907340
references.citation: J Clin Microbiol. 2004 Apr;42(4):1519-27
references.pmid: 15070998
references.citation: Oral Microbiol Immunol. 2000 Dec;15(6):350-4
references.pmid: 11154430
references.citation: J Clin Microbiol. 2005 Nov;43(11):5601-13
references.pmid: 16272493
references.citation: J Clin Microbiol. 1993 Jan;31(1):39-46
references.pmid: 8417030
references.citation: Mem Inst Oswaldo Cruz. 2004 Mar;99(2):147-52
references.pmid: 15250467
references.citation: J Clin Microbiol. 1997 Sep;35(9):2348-58
references.pmid: 9276415
references.citation: J Clin Microbiol. 2004 Sep;42(9):4025-31
references.pmid: 15364985
references.citation: Eukaryot Cell. 2007 Jun;6(6):1041-52
references.pmid: 17416899
references.citation: Antimicrob Agents Chemother. 2004 Jan;48(1):262-6
references.pmid: 14693548
references.citation: J Clin Microbiol. 2001 Nov;39(11):4076-81
references.pmid: 11682532
references.citation: J Clin Microbiol. 2003 Oct;41(10):4799-804
references.pmid: 14532225
references.citation: Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9456-9
references.pmid: 8415722
references.citation: Fungal Genet Biol. 2007 Dec;44(12):1298-309
references.pmid: 17625934
references.citation: J Clin Microbiol. 2004 Jun;42(6):2480-8
references.pmid: 15184424
references.citation: J Clin Microbiol. 2002 Aug;40(8):2729-40
references.pmid: 12149321
references.citation: J Clin Microbiol. 1995 Jun;33(6):1501-9
references.pmid: 7650175
references.citation: Clin Microbiol Rev. 2000 Apr;13(2):332-70
references.pmid: 10756003
references.citation: J Microbiol Methods. 2006 Mar;64(3):346-65
references.pmid: 16005996
references.citation: Science. 2006 Jul 21;313(5785):367-70
references.pmid: 16857942
references.citation: Genetics. 2005 Mar;169(3):1311-27
references.pmid: 15654090
references.citation: APMIS. 2002 Apr;110(4):315-24
references.pmid: 12076267
references.citation: J Clin Microbiol. 2002 Apr;40(4):1290-7
references.pmid: 11923347
references.citation: Mycoses. 2009 Jan;52(1):24-8
references.pmid: 18498300
references.citation: J Microbiol. 2004 Jun;42(2):80-6
references.pmid: 15357299
references.citation: Rev Iberoam Micol. 2002 Jun;19(2):89-94
references.pmid: 12828510
references.citation: J Clin Microbiol. 2003 Aug;41(8):3765-76
references.pmid: 12904388
references.citation: Clin Infect Dis. 2003 Nov 1;37(9):1172-7
references.pmid: 14557960
references.citation: J Clin Microbiol. 1993 Jun;31(6):1472-80
references.pmid: 8314989
references.citation: J Clin Microbiol. 2006 May;44(5):1810-20
references.pmid: 16672411
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002 Nov;94(5):589-92
references.pmid: 12424453
references.citation: Microbiology (Reading). 2004 Jul;150(Pt 7):2415-2428
references.pmid: 15256583
references.citation: Eukaryot Cell. 2008 Jul;7(7):1075-84
references.pmid: 18456859
references.citation: Microbiology (Reading). 2005 Mar;151(Pt 3):673-681
references.pmid: 15758214
references.citation: Med Mycol. 2006 Aug;44(5):405-17
references.pmid: 16882607
references.citation: J Clin Microbiol. 2005 Dec;43(12):6164-6
references.pmid: 16333121
references.citation: J Clin Microbiol. 2003 Nov;41(11):5265-6
references.pmid: 14605179
references.citation: Int J Syst Evol Microbiol. 2000 May;50 Pt 3:1343-1349
references.pmid: 10843081
references.citation: Microbiology (Reading). 1999 Oct;145 ( Pt 10):2635-46
references.pmid: 10537185
references.citation: Annu Rev Microbiol. 2006;60:561-88
references.pmid: 16774461
references.citation: Crit Rev Microbiol. 1987;15(1):97-101
references.pmid: 3319426
references.citation: Mycoses. 2007 Jan;50(1):21-4
references.pmid: 17302743
references.citation: J Clin Microbiol. 2004 Dec;42(12):5899-903
references.pmid: 15583336
references.citation: J Clin Microbiol. 1995 Nov;33(11):2920-6
references.pmid: 8576346
references.citation: J Clin Microbiol. 2006 Oct;44(10):3647-58
references.pmid: 17021093
references.citation: Sex Transm Infect. 2008 Apr;84(2):103-6
references.pmid: 17971371
references.citation: Fungal Genet Biol. 2004 May;41(5):553-62
references.pmid: 15050544
references.citation: Community Dent Oral Epidemiol. 2000 Apr;28(2):141-9
references.pmid: 10730723
references.citation: J Clin Microbiol. 2002 Mar;40(3):826-36
references.pmid: 11880401
references.citation: FEMS Immunol Med Microbiol. 2003 Oct 24;39(1):1-7
references.pmid: 14556989
references.citation: J Clin Microbiol. 2003 Dec;41(12):5709-17
references.pmid: 14662965
references.citation: Diagn Microbiol Infect Dis. 2005 Sep;53(1):29-31
references.pmid: 16182076
references.citation: Microbiology (Reading). 1999 May;145 ( Pt 5):1137-1143
references.pmid: 10376829
references.citation: Mycoses. 2004 Dec;47(11-12):495-502
references.pmid: 15601456
references.citation: J Clin Microbiol. 1997 Jun;35(6):1332-6
references.pmid: 9163439
references.citation: J Oral Pathol Med. 2002 Feb;31(2):99-105
references.pmid: 11896831
references.citation: Microbiology (Reading). 1999 Sep;145 ( Pt 9):2405-2413
references.pmid: 10517593
references.citation: J Clin Microbiol. 1994 Nov;32(11):2646-54
references.pmid: 7852550
references.citation: Clin Microbiol Rev. 2000 Jan;13(1):122-43, table of contents
references.pmid: 10627494
references.citation: J Clin Microbiol. 2003 Feb;41(2):552-7
references.pmid: 12574245