title: Media optimization for laccase production by Trichoderma harzianum ZF-2 using response surface methodology.
abstract: Trichoderma harzianum ZF-2 producing laccase was isolated from decaying samples from Shandong, China, and showed dye decolorization activities. The objective of this study was to optimize its culture conditions using a statistical analysis of its laccase production. The interactions between different fermentation parameters for laccase production were characterized using a Plackett-Burman design and the response surface methodology. The different media components were initially optimized using the conventional one-factor-at-a-time method and an orthogonal test design, and a Plackett-Burman experiment was then performed to evaluate the effects on laccase production. Wheat straw powder, soybean meal, and CuSO4 were all found to have a significant influence on laccase production, and the optimal concentrations of these three factors were then sequentially investigated using the response surface methodology with a central composite design. The resulting optimal medium components for laccase production were determined as follows: wheat straw powder 7.63 g/l, soybean meal 23.07 g/l, (NH4)2SO4 1 g/l, CuSO4 0.51 g/l, Tween-20 1 g/l, MgSO4 1 g/l, and KH2PO4 0.6 g/l. Using this optimized fermentation method, the yield of laccase was increased 59.68 times to 67.258 U/ml compared with the laccase production with an unoptimized medium. This is the first report on the statistical optimization of laccase production by Trichoderma harzianum ZF-2.
authors.affiliation: College of Forestry, Shandong Agricultural University, Shandong 271018, China.
authors.forename: Huiju
authors.initials: H
authors.lastname: Gao
authors.forename: Xiang
authors.initials: X
authors.lastname: Chu
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Zhou
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.forename: Zhimei
authors.initials: Z
authors.lastname: Mu
authors.forename: Qingxin
authors.initials: Q
authors.lastname: Liu
mesh_terms: D001709:Biotechnology / Q000379:methods; D002681:China; D003470:Culture Media / Q000737:chemistry*; D042845:Laccase / Q000096:biosynthesis*; D015233:Models, Statistical; D014242:Trichoderma / Q000201:enzymology* / Q000254:growth & development* / Q000302:isolation & purification
chemical_list: D003470:Culture Media; D042845:Laccase