title: Apoptotic effect of methanol extract of Picrasma quassioides by regulating specificity protein 1 in human cervical cancer cells.
abstract: In the present study, we examined the effects of methanol extracts of Picrasma quassioides (MEPQ) on apoptosis in human cervical cancer cells. The results showed that MEPQ decreased the viability and induced caspase-dependent apoptosis in HEp-2 cells. MEPQ decreased specificity protein 1 (Sp1) in HEp-2 cells, whereas Sp1 mRNA was not changed. We found that MEPQ reduced Sp1 protein through proteasome-dependent protein degradation, but not the inhibition of protein synthesis. Also, MEPQ increased the expressions of Bad and truncated Bid (t-Bid) but did not alter other Bcl-2 family members. The knock-down of Sp1 by both Sp1 interfering RNA and Mithramycin A, Sp1 specific inhibitor clearly increased Bad and t-Bid expression to decrease cell viability and induce apoptosis. In addition, MEPQ inhibited cell viability and induced apoptotic cell death through the modulation of Sp1 in KB cells. These results suggest that MEPQ may be a potential anticancer agent for human cervical cancer.
authors.affiliation: Department of Oral Pathology, School of Dentistry, Institute of Oral Bioscience, Chonbuk National University, Jeonju, Korea.
authors.forename: Hang-Eun
authors.initials: HE
authors.lastname: Lee
authors.forename: Eun-Sun
authors.initials: ES
authors.lastname: Choi
authors.forename: Ji-Ae
authors.initials: JA
authors.lastname: Shin
authors.forename: Lee-Han
authors.initials: LH
authors.lastname: Kim
authors.forename: Nam-Pyo
authors.initials: NP
authors.lastname: Cho
authors.forename: Sung-Dae
authors.initials: SD
authors.lastname: Cho
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D051023:BH3 Interacting Domain Death Agonist Protein / Q000378:metabolism; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006801:Humans; D000432:Methanol; D032270:Picrasma; D010936:Plant Extracts / Q000494:pharmacology*; D008926:Plicamycin / Q000031:analogs & derivatives / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D012997:Solvents; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology; D051025:bcl-Associated Death Protein / Q000378:metabolism
chemical_list: C494819:BAD protein, human; D051023:BH3 Interacting Domain Death Agonist Protein; C494814:BID protein, human; D010936:Plant Extracts; D034741:RNA, Small Interfering; D012997:Solvents; D016329:Sp1 Transcription Factor; D051025:bcl-Associated Death Protein; C066851:mithramycin A; D046988:Proteasome Endopeptidase Complex; D008926:Plicamycin; D000432:Methanol
keywords: Bad; Picrasma quassioides; apoptosis; cervical cancer; specificity protein 1; truncated Bid