title: Regulation of cell shape and adhesion by CD34.
abstract: We previously reported that expression of CD43/leukosialin induces cell rounding and microvillus formation via inhibition of cell adhesion. Here, we found that CD34, a cell surface sialomucin and marker for hematopoietic progenitor cells, also inhibited cell adhesion and induced cell rounding and microvillus formation. Forced expression of CD34-induced cell rounding, microvillus formation, and phosphorylation of ezrin/radixin/moesin (ERM) proteins in HEK293T cells, while inhibiting integrin-mediated cell re-attachment. Furthermore, CD34+ blood cells and KG-1 cells, which express endogenous CD34 on their surface, were spherical in shape, surrounded by microvilli, and non-adherent to substrata. In addition, cleavage of O-sialomucin augmented integrin-mediated cell adhesion of KG-1 cells. These results suggest the involvement of CD34 in the inhibition of integrin-mediated cell adhesion and formation of the cell surface structure. The inhibitory function of CD34 in cell adhesion may affect cell shape organization via phosphorylation of ERM proteins. Cellular structures such as the spherical shape and microvilli of CD34+ cells may also contribute to regulation of cell adhesion.
authors.affiliation: Research Center for Medical Glycoscience; National Institute of Advanced Industrial Science and Technology (AIST); Umezono, Tsukuba, Ibaraki, Japan; Health Research Institute; AIST; Nakouji, Amagasaki, Hyogo, Japan.
authors.forename: Hiroe
authors.initials: H
authors.lastname: Ohnishi
authors.affiliation: Department of Molecular Cell Biology, Institute of DNA Medicine, The Jikei University School of Medicine, Nishi-Shinbashi, Minato-ku, Tokyo 105, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sasaki
authors.affiliation: Research Center for Regenerative Medicine; Tokai University School of Medicine; Bohseidai, Isehara, Kanagawa, Japan.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: Department of Cell Transplantation and Regenerative Medicine; Tokai University School of Medicine; Bohseidai, Isehara, Kanagawa, Japan.
authors.forename: Shunichi
authors.initials: S
authors.lastname: Kato
authors.affiliation: Research Center for Regenerative Medicine; Tokai University School of Medicine; Bohseidai, Isehara, Kanagawa, Japan; Department of Hematology/Oncology; Tokai University School of Medicine; Bohseidai, Isehara, Kanagawa, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ando
authors.affiliation: Research Center for Medical Glycoscience; National Institute of Advanced Industrial Science and Technology (AIST); Umezono, Tsukuba, Ibaraki, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Narimatsu
authors.affiliation: Research Center for Medical Glycoscience; National Institute of Advanced Industrial Science and Technology (AIST); Umezono, Tsukuba, Ibaraki, Japan; Health Research Institute; AIST; Nakouji, Amagasaki, Hyogo, Japan.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Tachibana
mesh_terms: D018952:Antigens, CD34 / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion / Q000235:genetics*; D002462:Cell Membrane / Q000378:metabolism*; D048430:Cell Shape / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D016023:Integrins / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D016023:Integrins
keywords: CD34; ERM proteins; cell adhesion; cell rounding; microvilli; phosphorylation; sialomucin
references.citation: J Cell Biol. 1994 Jun;125(6):1371-84
references.pmid: 8207064
references.citation: Immunol Res. 2006;34(1):13-32
references.pmid: 16720896
references.citation: J Cell Sci. 2008 Nov 15;121(Pt 22):3683-92
references.pmid: 18987355
references.citation: Science. 1996 Jul 12;273(5272):242-5
references.pmid: 8662508
references.citation: Immunity. 2005 Jan;22(1):43-57
references.pmid: 15664158
references.citation: Exp Hematol. 1992 Jun;20(5):590-9
references.pmid: 1375160
references.citation: Blood. 1996 Jan 1;87(1):1-13
references.pmid: 8547630
references.citation: Blood. 1993 Feb 15;81(4):1001-8
references.pmid: 7679004
references.citation: Nat Cell Biol. 2007 Oct;9(10):1110-21
references.pmid: 17909522
references.citation: Int J Biochem Cell Biol. 2008;40(3):344-9
references.pmid: 17419089
references.citation: Immunol Res. 1999;20(2):89-99
references.pmid: 10580634
references.citation: Bone Marrow Transplant. 1996 Jun;17(6):985-91
references.pmid: 8807104
references.citation: Blood. 2003 Dec 1;102(12):3890-9
references.pmid: 12907449
references.citation: PLoS One. 2007 Feb 21;2(2):e237
references.pmid: 17311105
references.citation: Curr Biol. 2008 Jan 22;18(2):91-101
references.pmid: 18207738
references.citation: Blood. 2005 Sep 15;106(6):1901-10
references.pmid: 15890683
references.citation: J Immunol. 1992 Jan 1;148(1):267-71
references.pmid: 1370171
references.citation: Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12240-4
references.pmid: 8618877
references.citation: J Cell Biol. 1998 Feb 23;140(4):885-95
references.pmid: 9472040
references.citation: J Cell Biol. 2009 Feb 9;184(3):451-62
references.pmid: 19204146
references.citation: Blood. 1996 Jan 15;87(2):479-90
references.pmid: 8555469
references.citation: J Biol Chem. 1986 Sep 25;261(27):12779-86
references.pmid: 2943740
references.citation: Cell Adh Migr. 2011 Mar-Apr;5(2):199-206
references.pmid: 21343695
references.citation: Blood. 1996 May 1;87(9):3550-62
references.pmid: 8611677
references.citation: Blood. 1991 Apr 15;77(8):1717-22
references.pmid: 1707696
references.citation: J Clin Invest. 1988 Mar;81(3):951-5
references.pmid: 2893812
references.citation: J Immunol. 1999 Jul 15;163(2):563-8
references.pmid: 10395641
references.citation: Stem Cells. 2005 Aug;23(7):879-94
references.pmid: 15888687
references.citation: J Cell Biol. 2008 Feb 25;180(4):739-46
references.pmid: 18283112
references.citation: J Cell Biol. 1998 Feb 9;140(3):647-57
references.pmid: 9456324
references.citation: Cell Adh Migr. 2011 Mar-Apr;5(2):119-32
references.pmid: 21045567
references.citation: J Biol Regul Homeost Agents. 2001 Jan-Mar;15(1):1-13
references.pmid: 11388737