title: Saccharification of pumpkin residues by coculturing of Trichoderma reesei RUT-C30 and Phanerochaete chrysosporium Burdsall with delayed inoculation timing.
abstract: Trichoderma reesei and Phanerochaete chrysosporium with different lignocellulose-degrading enzyme systems have received much attention due to their ability to biodegrade lignocellulosic biomass. However, the synergistic effect of the two fungi on lignocellulose degradation is unknown. Herein, a cocultivation of T. reesei RUT-C30 and P. chrysosporium Burdsall for biodegradation of lignocellulosic pumpkin residues (PRS) was developed to produce soluble saccharide. Results indicated that a cocultivation of the two fungi with P. chrysosporium Burdsall inoculation delayed for 1.5 days produced the highest saccharide yield of 53.08% (w/w), and only 20.83% (w/w) of PRS were left after one batch of fermentation. In addition, this strategy increased the activities of secreted cellulases (endoglucanase, cellobiohydrolase, and β-glucosidase) and ligninases (lignin peroxidase and manganese peroxidase), which correlated to the increased saccharide yield. Besides, the resulting monosaccharides including glucose (1.23 mg/mL), xylose (0.13 mg/mL), arabinose (0.46 mg/mL), and fructose (0.21 mg/mL) from cocultures exhibited much higher yields than those from monoculture, which provides basal information for further fermentation research. This bioconversion of PRS into soluble sugars by cocultured fungal species provides a low cost method based on lignocellulose for potential biofuels or other bioproduct production.
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University , China Key Laboratory of Fruit and Vegetable Processing, Ministry of Agriculture, China Research Center for Fruit and Vegetable Processing Engineering, Ministry of Education, Beijing 100083, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.forename: Demei
authors.initials: D
authors.lastname: Meng
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Hu
authors.forename: Yuanying
authors.initials: Y
authors.lastname: Ni
authors.forename: Quanhong
authors.initials: Q
authors.lastname: Li
mesh_terms: D056804:Biofuels; D050260:Carbohydrate Metabolism; D002480:Cellulase / Q000378:metabolism; D044602:Cellulases / Q000378:metabolism; D028464:Cucurbita / Q000382:microbiology*; D005656:Fungal Proteins / Q000378:metabolism; D007218:Industrial Microbiology; D010544:Peroxidases / Q000378:metabolism; D020075:Phanerochaete / Q000201:enzymology / Q000378:metabolism*; D014242:Trichoderma / Q000201:enzymology / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D056804:Biofuels; D005656:Fungal Proteins; D010544:Peroxidases; C051129:manganese peroxidase; D044602:Cellulases; D001617:beta-Glucosidase; D002480:Cellulase