title: Detection of mutation by allele-specific loop-mediated isothermal amplification (AS-LAMP).
abstract: For effective control of pathogen-transmitting mosquitoes, precise surveillance data of mosquito distribution are essential. Recently, an increase of insecticide resistance due to the kdr mutation in Anopheles gambiae, a mosquito that transmits the malaria parasite, has been reported. With the aim of developing a simple and effective method for surveying resistant mosquitoes, LAMP was applied to the allele-specific detection of the kdr gene in An. gambiae. Allele-specific LAMP (AS-LAMP) method successfully distinguished the kdr homozygote from the heterozygote and the wild type. The robustness of AS-LAMP suggests its usefulness for routine identification of insects, not only mosquitoes but also other vectors and agricultural pests. Here we describe the method of AS-LAMP to detect mutation in Anopheles mosquitoes.
authors.affiliation: Department of Tropical Medicine, The Jikei University School of Medicine, Tokyo, Japan.
authors.forename: Hiroka
authors.initials: H
authors.lastname: Aonuma
authors.forename: Athanase
authors.initials: A
authors.lastname: Badolo
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Okado
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Kanuka
mesh_terms: D000483:Alleles; D000818:Animals; D000852:Anopheles / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D017344:Genes, Insect; D057898:Hemizygote; D007306:Insecticides / Q000494:pharmacology; D058889:Molecular Typing / Q000379:methods*; D009391:Nephelometry and Turbidimetry; D021141:Nucleic Acid Amplification Techniques
chemical_list: D017931:DNA Primers; D007306:Insecticides