title: Synergistic haemolytic activity and its correlation to phospholipase D productivity by Corynebacteruim pseudotuberculosis Egyptian isolates from sheep and buffaloes.
abstract: Fourteen isolates of Corynebacteruim pseudotuberculosis of them 7 were isolated from sheep with Caseous Lymphadenitis "biotype 1" and 7 isolated from buffaloes with Oedematous Skin Disease "biotype 2". All isolates were identified by standard microbiological techniques and by polymerase chain reaction targeting, 16S rRNA and phospholipase D genes. Synergistic haemolytic titers of all isolates were assayed by plate technique. The presences of phospholipase D gene in supernatants of all isolates were performed by sodium dodecyl sulfate polyacrylamide gel electrophoresis immunoblot technique by using hyperimmune serum raised in rabbit immunized with recombinant phospholipase D gene antigen. The concentration of phospholipase D gene was assayed by scanning the bound phospholipase D gene with specific antibodies that appeared at 31.5 kDa. Results presented that there is no correlation between titer of Synergistic haemolytic activity and the actual phospholipase D genes concentration in culture supernatants. Also results presented that Synergistic haemolytic activity and phospholipase D genes produced by biotype 2 (buffalo isolates) was generally higher than those by biotype 1(sheep isolates).
authors.affiliation: Biotechnology Center for Veterinary Services and Research (BCVSR), Faculty of Veterinary Medicine, Cairo University.
authors.forename: S A
authors.initials: SA
authors.lastname: Selim
authors.forename: W M
authors.initials: WM
authors.lastname: Mousa
authors.forename: K F
authors.initials: KF
authors.lastname: Mohamed
authors.forename: I M
authors.initials: IM
authors.lastname: Moussa
keywords: Caseous Lymphadenitis; Corynebacteruim pseudotuberculosis; Oedematous Skin Disease; Synergistic haemolysis; recombinant PLD
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