title: ABO blood-typing using an antibody array technique based on surface plasmon resonance imaging.
abstract: In this study, readily available antibodies that are used in standard agglutination tests were evaluated for their use in ABO blood typing by a surface plasmon resonance imaging (SPR imaging) technique. Five groups of antibodies, including mixed clones of anti-A, anti-B, and anti-AB, and single clones of anti-A and anti-B, were used to construct the five-line detection arrays using a multichannel flow cell in the SPR imager. The red blood cell (RBC) samples were applied to a multichannel flow cell that was orthogonal to the detection line arrays for blood group typing. We found that the blood samples were correctly grouped in less than 12 min by the SPR imaging technique, and the results were consistent with those of the standard agglutination technique for all 60 samples. We found that mixed clones of antibodies provided 33%-68% greater change in the SPR signal than the single-clone antibodies. Applying the SPR imaging technique using readily available antibodies may reduce the costs of the antibodies, shorten the measurement time, and increase the throughput.
authors.affiliation: Materials Science and Engineering Programme, Faculty of Science, Mahidol University, Rama 6 Rd., Phayathai, Rajathavee, Bangkok 10400, Thailand. toemsak.sri@mahidol.ac.th.
authors.forename: Nongluck
authors.initials: N
authors.lastname: Houngkamhang
authors.forename: Apirom
authors.initials: A
authors.lastname: Vongsakulyanon
authors.forename: Patjaree
authors.initials: P
authors.lastname: Peungthum
authors.forename: Krisda
authors.initials: K
authors.lastname: Sudprasert
authors.forename: Pimpun
authors.initials: P
authors.lastname: Kitpoka
authors.forename: Mongkol
authors.initials: M
authors.lastname: Kunakorn
authors.forename: Boonsong
authors.initials: B
authors.lastname: Sutapun
authors.forename: Ratthasart
authors.initials: R
authors.lastname: Amarit
authors.forename: Armote
authors.initials: A
authors.lastname: Somboonkaew
authors.forename: Toemsak
authors.initials: T
authors.lastname: Srikhirin
mesh_terms: D000017:ABO Blood-Group System / Q000097:blood*; D000906:Antibodies / Q000276:immunology*; D015374:Biosensing Techniques / Q000295:instrumentation; D001788:Blood Grouping and Crossmatching / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D044085:Microfluidics / Q000295:instrumentation*; D040081:Protein Array Analysis / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
chemical_list: D000017:ABO Blood-Group System; D000906:Antibodies
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