title: A multiplexed assay to detect antimicrobial peptides in biological fluids and cell secretions.
abstract: Mucosal tissues represent the front line in defense against potential pathogens, and one means by which mucosa provide protection is via the secretion of antimicrobials which can interfere with potential pathogens as well as recruit and modify the responses of immune cells. Here we describe adaptation of ELISA assays to microsphere format, facilitating simultaneous quantification of antimicrobial peptides including elafin, MIP3α, HBD2, HBD3, SLPI, RANTES, SDF1, lactoferrin, LL-37, and HNP1-3. The multiplexed assay exhibits excellent reproducibility, shows linearity over a two order of magnitude concentration range for most analytes, is compatible with biological fluids such as cervicovaginal lavage fluid, and presents significant cost and sample savings relative to traditional ELISA assays.
authors.affiliation: Thayer School of Engineering, Dartmouth College, Hanover, NH, USA.
authors.forename: Austin W
authors.initials: AW
authors.lastname: Boesch
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Zhao
authors.forename: Alison S
authors.initials: AS
authors.lastname: Landman
authors.forename: Marta Rodriguez
authors.initials: MR
authors.lastname: Garcia
authors.forename: John V
authors.initials: JV
authors.lastname: Fahey
authors.forename: Charles R
authors.initials: CR
authors.lastname: Wira
authors.forename: Margaret E
authors.initials: ME
authors.lastname: Ackerman
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000032:analysis* / Q000378:metabolism*; D001826:Body Fluids / Q000737:chemistry* / Q000166:cytology* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans
chemical_list: D023181:Antimicrobial Cationic Peptides
keywords: Antimicrobials; Chemokines; Cytokines; Female reproductive tract; Mucosal secretions; Multiplex assay
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