title: Construction of human antibody gene libraries and selection of antibodies by phage display.
abstract: Antibody phage display is the most commonly used in vitro selection technology and has yielded thousands of useful antibodies for research, diagnostics, and therapy.The prerequisite for successful generation and development of human recombinant antibodies using phage display is the construction of a high-quality antibody gene library. Here, we describe the methods for the construction of human immune and naive scFv gene libraries.The success also depends on the panning strategy for the selection of binders from these libraries. In this article, we describe a panning strategy that is high-throughput compatible and allows parallel selection in microtiter plates.
authors.affiliation: Abteilung Biotechnologie Technische Universität Braunschweig, Institut für Biochemie, Biotechnologie und Bioinformatik, Braunschweig, Germany.
authors.forename: André
authors.initials: A
authors.lastname: Frenzel
authors.forename: Jonas
authors.initials: J
authors.lastname: Kügler
authors.forename: Sonja
authors.initials: S
authors.lastname: Wilke
authors.forename: Thomas
authors.initials: T
authors.lastname: Schirrmann
authors.forename: Michael
authors.initials: M
authors.lastname: Hust
mesh_terms: D000818:Animals; D061067:Antibodies, Monoclonal, Humanized / Q000235:genetics* / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D061505:Cell Surface Display Techniques; D015723:Gene Library; D006801:Humans; D019151:Peptide Library; D015202:Protein Engineering / Q000379:methods; D057127:Single-Chain Antibodies / Q000235:genetics / Q000276:immunology
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D019151:Peptide Library; D057127:Single-Chain Antibodies