title: Antiproliferative cardiac glycosides from the latex of Antiaris toxicaria.
abstract: Phytochemical investigation of the latex of Antiaris toxicaria resulted in the isolation of 15 new [antiarosides J-X (1-15)] and 17 known cardiac glycosides. The effects of the cardiac glycosides on apoptosis and the expression of orphan nuclear receptor Nur77 were examined in human NIH-H460 lung cancer cells. Several of the cardiac glycosides induced apoptosis in lung cancer cells, which was accompanied by induction of Nur77 protein expression. Treatment of cancer cells with the cardiac glycosides resulted in translocation of the Nur77 protein from the nucleus to the cytoplasm and subsequent targeting to mitochondria. The results show that the cardiac glycosides exert their apoptotic effect through the Nur77-dependent apoptotic pathway.
authors.affiliation: Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University , Guangzhou 510632, People's Republic of China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Jin-Shan
authors.initials: JS
authors.lastname: Tang
authors.forename: Meng-Jie
authors.initials: MJ
authors.lastname: Hu
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Chen
authors.forename: Hao
authors.initials: H
authors.lastname: Gao
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Wang
authors.forename: Shun-Lin
authors.initials: SL
authors.lastname: Li
authors.forename: Xiao-Jiang
authors.initials: XJ
authors.lastname: Hao
authors.forename: Xiao-Kun
authors.initials: XK
authors.lastname: Zhang
authors.forename: Xin-Sheng
authors.initials: XS
authors.lastname: Yao
mesh_terms: D031621:Antiaris / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002301:Cardiac Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D007840:Latex / Q000737:chemistry*; D008928:Mitochondria / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1 / Q000187:drug effects
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002301:Cardiac Glycosides; D004365:Drugs, Chinese Herbal; D007840:Latex; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1