title: Compromise of endogenous neuropeptide W production abrogates the dipsogenic and pressor effects of angiotensin II in adult male rats.
abstract: Neuropeptide W (NPW), an endogenous ligand for the G-protein coupled receptor GPR7, is produced in neurones in the rat hypothalamus and brain stem known to be important in the control of food intake and the neuroendocrine response to stress. In previous studies, central administration of NPW during the light phase increased food and water intake and elevated prolactin and corticosterone levels in conscious, unrestrained male rats. In the present study, central administration of small-interfering RNA (siRNA) reduced NPW levels in the hypothalamus and resulted in a failure of angiotensin II to stimulate water drinking or increase mean arterial pressure. In addition, siRNA-treated animals failed to mount a significant prolactin response to immobilisation stress, at the same time as maintaining a normal corticosterone response. These results suggest that endogenous NPW may be a physiologically relevant, downstream mediator of the central actions of angiotensin II to stimulate thirst and increase arterial pressure. In addition, NPW-producing neurones appear to participate in the hypothalamic mechanisms controlling prolactin (but not corticosterone) secretion.
authors.affiliation: Department of Pharmacological and Physiological Science, Saint Louis University, Saint Louis, MO, USA.
authors.forename: A T
authors.initials: AT
authors.lastname: Pate
authors.affiliation: Department of Pharmacological and Physiological Science, Saint Louis University, Saint Louis, MO, USA.
authors.forename: G L C
authors.initials: GLC
authors.lastname: Yosten
authors.affiliation: Department of Pharmacological and Physiological Science, Saint Louis University, Saint Louis, MO, USA.
authors.forename: W K
authors.initials: WK
authors.lastname: Samson
mesh_terms: D000804:Angiotensin II / Q000502:physiology*; D000818:Animals; D001794:Blood Pressure / Q000502:physiology*; D004327:Drinking Behavior / Q000502:physiology*; D008297:Male; D009479:Neuropeptides / Q000096:biosynthesis* / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009479:Neuropeptides; D034741:RNA, Small Interfering; C496874:neuropeptide W, rat; D000804:Angiotensin II
keywords: angiotensin II; blood pressure; neuropeptide W; stress hormones; water drinking
references.citation: J Neurosci. 2011 Oct 19;31(42):15009-15
references.pmid: 22016534
references.citation: Life Sci. 1981 Oct 5;29(14):1425-8
references.pmid: 7300559
references.citation: Am J Physiol Regul Integr Comp Physiol. 2013 Oct 1;305(7):R804-10
references.pmid: 23926134
references.citation: Neuropeptides. 2010 Apr;44(2):99-106
references.pmid: 19948359
references.citation: Am J Physiol. 1992 Dec;263(6 Pt 2):R1347-53
references.pmid: 1336319
references.citation: Endocrinology. 1985 Feb;116(2):723-7
references.pmid: 2981677
references.citation: Proc Natl Acad Sci U S A. 2003 Sep 2;100(18):10540-5
references.pmid: 12925742
references.citation: Br J Pharmacol. 2006 Aug;148(8):1033-41
references.pmid: 16847439
references.citation: Neurosci Lett. 2003 Oct 2;349(2):71-4
references.pmid: 12946555
references.citation: J Comp Neurol. 2006 Jul 20;497(3):367-83
references.pmid: 16736466
references.citation: Proc Natl Acad Sci U S A. 2005 Jul 12;102(28):9942-7
references.pmid: 15983370
references.citation: Genomics. 1995 Jul 1;28(1):84-91
references.pmid: 7590751
references.citation: Brain Res. 2004 Aug 13;1017(1-2):222-6
references.pmid: 15261118
references.citation: Am J Physiol Regul Integr Comp Physiol. 2007 Jan;292(1):R382-7
references.pmid: 16902182
references.citation: Endocrinology. 1991 Oct;129(4):1744-8
references.pmid: 1915064
references.citation: Endocrinology. 2010 May;151(5):2200-10
references.pmid: 20189998
references.citation: J Neuroendocrinol. 2004 Oct;16(10):842-9
references.pmid: 15500544
references.citation: Am J Physiol Regul Integr Comp Physiol. 2005 Jun;288(6):R1727-32
references.pmid: 15886360
references.citation: J Physiol. 1977 Apr;266(2):453-69
references.pmid: 857006
references.citation: Brain Res Mol Brain Res. 1999 Jul 23;71(1):96-103
references.pmid: 10407191
references.citation: Am J Physiol Regul Integr Comp Physiol. 2009 Aug;297(2):R330-6
references.pmid: 19474390
references.citation: Endocrinology. 2003 Jul;144(7):2816-21
references.pmid: 12810535
references.citation: Am J Cardiol. 2002 Jan 24;89(2A):3A-9A; discussion 10A
references.pmid: 11835903
references.citation: Endocrinology. 1992 Apr;130(4):2183-91
references.pmid: 1312449
references.citation: J Appl Physiol. 1974 Mar;36(3):391-2
references.pmid: 4814312
references.citation: J Neuroendocrinol. 2009 Oct;21(10):850-7
references.pmid: 19686447
references.citation: Endocrinology. 2003 Nov;144(11):4729-33
references.pmid: 12959997
references.citation: Am J Physiol Regul Integr Comp Physiol. 2005 Jan;288(1):R270-5
references.pmid: 15345475
references.citation: Am J Physiol Regul Integr Comp Physiol. 2005 Jul;289(1):R52-8
references.pmid: 15831764
references.citation: Brain Res. 2006 Jun 6;1093(1):123-34
references.pmid: 16697979