title: Genomewide screening for genes involved in biofilm formation and miconazole susceptibility in Saccharomyces cerevisiae.
abstract: Infections related to fungal biofilms are difficult to treat due to the reduced susceptibility of sessile cells to most antifungal agents. Previous research has shown that 1-10% of sessile Candida cells survive treatment with high doses of miconazole (a fungicidal imidazole). The aim of this study was to identify genes involved in fungal biofilm formation and to unravel the mechanisms of resistance of these biofilms to miconazole. To this end, a screening of a Saccharomyces cerevisiae deletion mutant bank was carried out. Our results revealed that genes involved in peroxisomal transport and the biogenesis of the respiratory chain complex IV play an essential role in biofilm formation. On the other hand, genes involved in transcription and peroxisomal and mitochondrial organization seem to highly influence the susceptibility to miconazole of yeast biofilms. Additionally, our data confirm previous findings on genes involved in biofilm formation and in general stress responses. Our data suggest the involvement of peroxisomes in biofilm formation and miconazole resistance in fungal biofilms.
authors.affiliation: Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium.
authors.forename: Davy
authors.initials: D
authors.lastname: Vandenbosch
authors.forename: Evelien
authors.initials: E
authors.lastname: De Canck
authors.forename: Inne
authors.initials: I
authors.lastname: Dhondt
authors.forename: Petra
authors.initials: P
authors.lastname: Rigole
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Nelis
authors.forename: Tom
authors.initials: T
authors.lastname: Coenye
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms; D002176:Candida albicans / Q000187:drug effects / Q000502:physiology; D025141:Drug Resistance, Fungal / Q000235:genetics; D005800:Genes, Fungal; D005820:Genetic Testing; D055106:Genome-Wide Association Study; D008825:Miconazole / Q000494:pharmacology*; D009154:Mutation; D020675:Peroxisomes; D017382:Reactive Oxygen Species; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000502:physiology*
chemical_list: D000935:Antifungal Agents; D017382:Reactive Oxygen Species; D008825:Miconazole
keywords: peroxisome; resistance; yeast