title: [Establishment of a purificatory method for alpha-fetoprotein variant by affinity adsorption].
abstract: OBJECTIVE
  To establish a purificatory method of alpha-fetoprotein variant (AFP-L3) based on microspincolumn with lens culinaris agglutinin (LCA).
  
  
  METHODS
  LCA was isolated by ammonium sulfate precipitation method from lens culinaris. AFP-L3 affinity adsorption microspincolumns which were made from LCA coupled with activated Sepharose 4B were prepared. By adding into the centrifuge column, serum was absorbed and eluted to purify AFP-L3. The results of purified AFP-L3 detection of 10 cases AFP positive sera by electro-chemiluminescence immunoassay were compared with traditional crossed affinity immunoelectrophoresis.
  
  
  RESULTS
  8 of 10 cases AFP-L3 concentration were greater than 5 ng/ml in purified sera. Six cases show positive reaction in affinity immune cross electrophoresis experiment.
  
  
  CONCLUSIONS
  Successfully established purification method of AFP-L3 by affinity absorption based on microspincolumn. The method was more conducive to clinical laboratory applications due to its high sensitive and easy operation.
authors.affiliation: Center of Clinical Laboratory, No. 302 Hospital of the PLA, Beijing 100039, China.
authors.forename: Yong-Ji
authors.initials: YJ
authors.lastname: Song
authors.forename: Jun
authors.initials: J
authors.lastname: Hou
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.forename: Ai-Xia
authors.initials: AX
authors.lastname: Liu
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Jing-Xia
authors.initials: JX
authors.lastname: Guo
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Jing-Xiao
authors.initials: JX
authors.lastname: Yan
authors.forename: Bo-An
authors.initials: BA
authors.lastname: Li
authors.forename: Yuan-Li
authors.initials: YL
authors.lastname: Mao
mesh_terms: D000327:Adsorption; D002846:Chromatography, Affinity / Q000379:methods*; D007122:Immunoelectrophoresis; D027806:Lens Plant; D037121:Plant Lectins / Q000737:chemistry; D015203:Reproducibility of Results; D000509:alpha-Fetoproteins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D037121:Plant Lectins; D000509:alpha-Fetoproteins