title: Ribosomal DNA nanoprobes studied by Fourier transform infrared spectroscopy.
abstract: Paracoccidioides brasiliensis (P. brasiliensis) is a thermo-dimorphic fungus that causes paracoccidioidomycosis. Brazil epidemiological data shows that endemic areas are the subtropical regions, especially where agricultural activities predominate such as the Southeast, South, and Midwest. There are several tests to diagnose paracoccidioidomycosis, but they have many limitations such as low sensitivity, high cost, and a cross-reacting problem. In this work, gold nanoprobes were used to identify P. brasiliensis as an alternative diagnostic technique, which is easier to apply, costs less, and has great potential for application. The specific Ribosomal sequence of P. brasiliensis DNA was amplified and used to design the nanoprobes using a thiol-modified oligonucleotide. The results of positive and negative tests were done by UV-visible and Fourier Transform Infrared (FT-IR) measurements. The deconvolution of FT-IR sample spectra showed differences in the vibrational modes from the hydrogen bridge NHN and NHO bands that form the double helix DNA for samples matching the DNA sequence of nanoprobes that could be used to classify the samples.
authors.affiliation: Institute of Research and Development - IP&D, Universidade do Vale do Paraíba - UniVap, Shishima Hifumi Avenue, 2911, 12244-000 São José dos Campos, São Paulo, Brazil.
authors.forename: Jaciara
authors.initials: J
authors.lastname: Fagundes
authors.forename: Maiara L
authors.initials: ML
authors.lastname: Castilho
authors.forename: Claudio A
authors.initials: CA
authors.lastname: Téllez Soto
authors.forename: Laís de Souza
authors.initials: Lde S
authors.lastname: Vieira
authors.forename: Renata A
authors.initials: RA
authors.lastname: Canevari
authors.forename: Priscila P
authors.initials: PP
authors.lastname: Fávero
authors.forename: Airton A
authors.initials: AA
authors.lastname: Martin
authors.forename: Leandro
authors.initials: L
authors.lastname: Raniero
mesh_terms: D004271:DNA, Fungal / Q000032:analysis* / Q000737:chemistry; D004275:DNA, Ribosomal / Q000032:analysis* / Q000737:chemistry; D015335:Molecular Probes / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D014732:Vibration
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D015335:Molecular Probes
keywords: FT-Infrared spectra; Paracoccidioides brasiliensis (P. brasiliensis); Ribosomal DNA Nanoprobes