title: Semen cryopreservation for men banking for oligospermia, cancers, and other pathologies: prediction of post-thaw outcome using basal semen quality.
abstract: OBJECTIVE
  To evaluate post-thawing sperm parameters in a large series of men cryopreserving for different cancers and oligospermia.
  
  
  METHODS
  Retrospective observational study.
  
  
  METHODS
  Semen cryopreservation laboratory.
  
  
  METHODS
  Six hundred twenty-three patients undergoing semen cryopreservation for cancer or oligospermia who discontinued banking.
  
  
  METHODS
  None.
  
  
  METHODS
  Postcryopreservation sperm motility and viability.
  
  
  RESULTS
  In oligospermic men, recovery of motile sperm after cryopreservation was possible in only a few out of the 219 samples cryopreserved for this problem. Similarly, independent of the reason for which cryopreservation was required, if one basal semen parameter fell below the 5th percentile of the World Health Organization reference values, recovery of motile and viable spermatozoa after thawing was low. Among samples cryopreserved for cancer, those with testicular cancer showed the lowest basal semen quality and recovery after thawing. In cases of hematological cancers or other types of cancers, motility recovery was similar to that of non-cancer-related samples. Receiver operating characteristic analyses demonstrate that basal progressive and total motility predict the recovery rate of motile sperm after thawing with high accuracy, sensibility and specificity.
  
  
  CONCLUSIONS
  Our study demonstrates the ability of prefreeze semen parameters to predict cryosurvival in terms of sensitivity and precision. Using this information, the clinician could perform appropriate counseling about the future possibilities of fertility for the patient.
authors.affiliation: Department of Biomedical Experimental and Clinical Science, Sexual Medicine and Andrology Unit, University of Florence and Azienda Ospedaliera, Florence, Italy.
authors.forename: Selene
authors.initials: S
authors.lastname: Degl'Innocenti
authors.forename: Erminio
authors.initials: E
authors.lastname: Filimberti
authors.forename: Angela
authors.initials: A
authors.lastname: Magini
authors.forename: Csilla
authors.initials: C
authors.lastname: Krausz
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Lombardi
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Fino
authors.forename: Giulia
authors.initials: G
authors.lastname: Rastrelli
authors.forename: Mario
authors.initials: M
authors.lastname: Maggi
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Baldi
mesh_terms: D000328:Adult; D002470:Cell Survival; D015925:Cryopreservation / Q000706:statistics & numerical data*; D006801:Humans; D007558:Italy / Q000453:epidemiology; D008297:Male; D009369:Neoplasms / Q000453:epidemiology / Q000473:pathology*; D009845:Oligospermia / Q000453:epidemiology / Q000473:pathology*; D015995:Prevalence; D012662:Semen Preservation / Q000706:statistics & numerical data*; D013048:Specimen Handling; D013074:Sperm Banks / Q000706:statistics & numerical data*; D013081:Sperm Motility; D013094:Spermatozoa / Q000473:pathology*; D013696:Temperature
keywords: Sperm cryopreservation; cancer; oligospermia; sperm motility; sperm viability