title: Photodynamic inactivation of clinical isolates of Candida using Photodithazine®.
abstract: This study evaluated the photodynamic inactivation (PDI) mediated by Photodithazine(®) (PDZ) against 15 clinical isolates of Candida albicans, Candida glabrata and Candida tropicalis. Each isolate, in planktonic and biofilm form, was exposed to PDI by assessing a range of PDZ concentrations and light emitting diode fluences. Cell survival of the planktonic suspensions was determined by colony forming units (CFU ml(-1)). The antifungal effects of PDI against biofilms were evaluated by CFU ml(-1) and metabolic assay. Data were analyzed by non-parametric tests (α = 0.05). Regardless of the species, PDI promoted a significant viability reduction of planktonic yeasts. The highest reduction in cell viability of the biofilms was equivalent to 0.9 log10 (CFU ml(-1)) for C. albicans, while 1.4 and 1.5 log10 reductions were obtained for C. tropicalis and C. glabrata, respectively. PDI reduced the metabolic activity of biofilms by 62.1, 76.0, and 76.9% for C. albicans, C. tropicalis, and C. glabrata, respectively. PDZ-mediated PDI promoted significant reduction in the viability of Candida isolates.
authors.affiliation: a Department of Social Dentistry , Araraquara Dental School, UNESP - Univ Estadual Paulista , Araraquara , Brazil .
authors.forename: L N
authors.initials: LN
authors.lastname: Dovigo
authors.forename: J C
authors.initials: JC
authors.lastname: Carmello
authors.forename: M T
authors.initials: MT
authors.lastname: Carvalho
authors.forename: E G
authors.initials: EG
authors.lastname: Mima
authors.forename: C E
authors.initials: CE
authors.lastname: Vergani
authors.forename: V S
authors.initials: VS
authors.lastname: Bagnato
authors.forename: A C
authors.initials: AC
authors.lastname: Pavarina
mesh_terms: D018441:Biofilms / Q000187:drug effects* / Q000528:radiation effects*; D002175:Candida / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D002176:Candida albicans; D041221:Candida glabrata; D041022:Candida tropicalis; D004305:Dose-Response Relationship, Drug; D005659:Fungicides, Industrial / Q000494:pharmacology*; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D010933:Plankton / Q000187:drug effects / Q000382:microbiology / Q000528:radiation effects; D013045:Species Specificity
chemical_list: D005659:Fungicides, Industrial; C047635:N-methylglucosamine; D005944:Glucosamine