title: Supraglottic laryngeal tumor microenvironmental factors facilitate STAT3 dependent pro-tumorigenic switch in tumor associated macrophages to render utmost immune evasion.
abstract: Content of tumor microenvironment (TME) is varied greatly among different types of laryngeal tumors, namely, supraglottic, glottic and subglottic tumors. These three different TMEs shape infiltrating monocytes/macrophages toward M2 genotypes in variable degrees. Results obtained from in vitro studies demonstrated extent of expression of M2 phenotypic features on macrophages was maximum after their exposure to supraglottic laryngeal tumor cell lysates (SLTCL) than glottic or subglottic lysates. Moreover, M2 macrophages generated under influence of SLTCL show less nitric oxide production, greater IL-10: IL-12 ratio and poor antigen presentation. Co-culture of such M2 macrophages with T cells from healthy donors resulted decreased activation of T cells and T cell mediated tumor cell cytotoxicity, than, glottic or subglottic. SLTCL mediated macrophage polarization is STAT3 dependent and might be one of the major factors for severe immune paralysis leading to poor prognosis of supraglottic laryngeal tumor bearer following standard treatment.
authors.affiliation: Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute (CNCI), 37, S. P. Mukherjee Road, Kolkata 700026, India.
authors.forename: Kuntal Kanti
authors.initials: KK
authors.lastname: Goswami
authors.forename: Subhasis
authors.initials: S
authors.lastname: Barik
authors.forename: Saptak
authors.initials: S
authors.lastname: Banerjee
authors.forename: Anup Kumar
authors.initials: AK
authors.lastname: Bhowmick
authors.forename: Jaydip
authors.initials: J
authors.lastname: Biswas
authors.forename: Anamika
authors.initials: A
authors.lastname: Bose
authors.forename: Rathindranath
authors.initials: R
authors.lastname: Baral
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000187:drug effects / Q000276:immunology; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D018920:Coculture Techniques; D003602:Cytotoxicity, Immunologic; D004825:Epiglottis / Q000378:metabolism / Q000473:pathology; D005931:Glottis / Q000378:metabolism / Q000473:pathology; D006801:Humans; D057131:Immune Evasion / Q000187:drug effects / Q000276:immunology; D007150:Immunohistochemistry; D007155:Immunologic Factors / Q000378:metabolism* / Q000494:pharmacology; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D018664:Interleukin-12 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008213:Lymphocyte Activation / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000276:immunology / Q000378:metabolism; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology; D014020:Tissue Extracts / Q000378:metabolism* / Q000494:pharmacology; D059016:Tumor Microenvironment
chemical_list: D007155:Immunologic Factors; D050796:STAT3 Transcription Factor; D014020:Tissue Extracts; D016753:Interleukin-10; D018664:Interleukin-12; D009569:Nitric Oxide
keywords: IL-10; M2 macrophages; Prognosis; STAT3; Supraglottic laryngeal tumor