title: Biosynthesis of sucrose-6-acetate catalyzed by surfactant-coated Candida rugosa lipase immobilized on sol-gel supports.
abstract: Sucrose-6-acetate is an important intermediate in the preparation of sucralose (a finest sweetener). In our study, Candida rugosa lipase coated with surfactant was firstly immobilized on sol-gel supports. Then, the immobilized enzyme was used in the regioselective synthesis of sucrose-6-acetate by transesterification of sucrose and vinyl acetate. The screening results revealed that Tween 80 was an ideal surfactant to coat lipase immobilized in sol-gel and exhibited the highest yield of sucrose-6-acetate. Other factors that influenced the yield during the preparation process were also studied. Under optimal conditions, the yield of sucrose-6-acetate could reach up to 78.68 %, while free lipase was easily inactivated in polar solvent. Thermal and operational stabilities were also improved significantly. Surfactant-coated lipase immobilized in sol-gel remained stable when the temperature was higher than 60 °C. Moreover, they could maintain high catalytic activity after six recycles. This strategy is economical, convenient and promising for the food industry.
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhong
authors.forename: Junqing
authors.initials: J
authors.lastname: Qian
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Hu
authors.forename: Min
authors.initials: M
authors.lastname: Liu
mesh_terms: D002175:Candida; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D011136:Polysorbates / Q000737:chemistry*; D013395:Sucrose / Q000031:analogs & derivatives* / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D011136:Polysorbates; D013501:Surface-Active Agents; C556997:sucrose-6-acetate; D013395:Sucrose; D008049:Lipase