title: The osmoprotective effect of some organic solutes on Streptomyces sp. mado2 and nocardiopsis sp. mado3 growth.
abstract: The response of two marine actinomycetes such as Streptomyces sp. MADO2 and Nocardiopsis sp. MADO3 to osmotic stress in minimal medium M63 and in glycerol-asparagine medium (ISP5) was studied. The two strains were moderately halophilic and the behavior of the strain Streptomyces sp. MADO2 and Nocardiopsis sp. MADO3 towards the salt stress was varied depends on the media composition and the salinity concentration. The strain Streptomyces sp. was more sensitive to salt stress than Nocardiopsis sp. The growth of both Streptomyces sp. and Nocardiopsis sp. were inhibited at 1 M NaCl irrespective of the medium used. The Nocardiopsis sp. acquired osmoadaptation on ISP5 medium whereas the Streptomyces sp. showed poor growth on M63 medium. Glycine betaine (GB), proline and trehalose played a critical role in osmotic adaptation at high osmolarity whereas at low osmolarity they showed an inhibitory effect on the bacterial growth. The present findings confirmed that GB was the powerful osmoprotectant for Streptomyces sp. and Nocardiopsis sp. grown at 1 M NaCl both in M63 and ISP5 media.
authors.affiliation: Laboratoire d'écologie microbienne, Département de Biologie, Faculté des sciences , Université Ferhat Abbas, Sétif 19000 , Algeria.
authors.forename: Hanane
authors.initials: H
authors.lastname: Ameur
authors.forename: Mostefa
authors.initials: M
authors.lastname: Ghoul
authors.forename: Joseph
authors.initials: J
authors.lastname: Selvin
keywords: ISP5 medium; M63 medium; Nocardiopsis sp.,; Streptomyces sp.,; osmoprotective effect
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