title: Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates.
abstract: The emergence of Extended-Spectrum Beta-Lactamase (ESBL)-producing microorganisms in Brazilian hospitals is a challenge that concerns scientists, clinicians and healthcare institutions due to the serious risk they pose to confined patients. The goal of this study was the detection of ESBL production by clinical strains of Escherichia coli and Klebsiella sp. isolated from pus, urine and blood of patients at Hospital Universitário Santa Maria, Rio Grande Sul, RS, Brazil and the genotyping of the isolates based on bla SHV genes. The ESBL study was carried out using the Combined Disc Method, while Polymerase Chain Reaction (PCR) was used to study the bla SHV genes. Of the 90 tested isolates, 55 (61.1%) were identified as ESBL-producing by the combined disk method. The bla SHV genes were found in 67.8% of these microorganisms. K. pneumoniae predominated in the samples, presenting the highest frequency of positive results from the combined disk and PCR.
authors.affiliation: Programa de Pós graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria , Santa Maria, RS , Brasil.
authors.forename: Caio Fernando
authors.initials: CF
authors.lastname: de Oliveira
authors.forename: Adenilde
authors.initials: A
authors.lastname: Salla
authors.forename: Valéria Maria
authors.initials: VM
authors.lastname: Lara
authors.forename: Alexandre
authors.initials: A
authors.lastname: Rieger
authors.forename: Jorge André
authors.initials: JA
authors.lastname: Horta
authors.forename: Sydney Hartz
authors.initials: SH
authors.lastname: Alves
keywords: ESBL; Enterobacteriaceae; PCR; Prevalence
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