title: Rapid cloning and comparative sequence analysis of full-length cDNA of Rhesus monkey (Macaca mulatta) resistin.
abstract: Resistin protein is thought to link insulin resistance in murine models of obesity and type-2 diabetes, but the role of resistin in human studies of inflammatory metabolic disorders have generated conflicting data. Here, we describe the structure of the resistin gene using adipose tissue from non-human primates (NHPs), which have been used extensively to model a host of human diseases. Full-length cDNA from rhesus macaque resistin obtained by rapid amplification of cDNA ends (RACE) is comprised of 526 nucleotides covering an open-reading frame (ORF) that encodes a 108-amino-acid protein that is 92% homologous with the human counterpart but only 60% homologous with the murine counterpart. Using a modified polymerase chain reaction technique, we identified single nucleotide polymorphisms and a 78-bp deletion within resistin cDNA of nine rhesus macaques. Comparisons of the full-length cDNA sequence and an amplified 569-bp genomic DNA sequence revealed an error in published predictions arising from genomic studies about the gene's exon 3 region. Our data show, for the first time, the full-length macaque resistin cDNA sequence (GenBank: JF740676.1). These findings will illuminate future studies into the role of resistin in NHP models of inflammatory metabolic diseases.
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming, China.
authors.forename: Shuai-Yao
authors.initials: SY
authors.lastname: Lu
authors.forename: Zhan-Long
authors.initials: ZL
authors.lastname: He
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Sun
authors.forename: Hong-Jun
authors.initials: HJ
authors.lastname: Li
authors.forename: Pei-Fu
authors.initials: PF
authors.lastname: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D008253:Macaca mulatta / Q000235:genetics* / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; C076744:cytoplasmic linker protein 170
keywords: coding DNA sequence (CDS); gene polymorphisms; insulin resistance; resistin; rhesus macaque; splice variants