title: Genes for iron-sulphur cluster assembly are targets of abiotic stress in rice, Oryza sativa.
abstract: Iron-sulphur (Fe-S) cluster assembly occurs in chloroplasts, mitochondria and cytosol, involving dozens of genes in higher plants. In this study, we have identified 41 putative Fe-S cluster assembly genes in rice (Oryza sativa) genome, and the expression of all genes was verified. To investigate the role of Fe-S cluster assembly as a metabolic pathway, we applied abiotic stresses to rice seedlings and analysed Fe-S cluster assembly gene expression by qRT-PCR. Our data showed that genes for Fe-S cluster assembly in chloroplasts of leaves are particularly sensitive to heavy metal treatments, and that Fe-S cluster assembly genes in roots were up-regulated in response to iron toxicity, oxidative stress and some heavy metal assault. The effect of each stress treatment on the Fe-S cluster assembly machinery demonstrated an unexpected tissue or organelle specificity, suggesting that the physiological relevance of the Fe-S cluster assembly is more complex than thought. Furthermore, our results may reveal potential candidate genes for molecular breeding of rice.
authors.affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Liang
authors.forename: Lu
authors.initials: L
authors.lastname: Qin
authors.forename: Peiwei
authors.initials: P
authors.lastname: Liu
authors.forename: Meihuan
authors.initials: M
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Ye
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics; D002736:Chloroplasts / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics*; D007506:Iron-Sulfur Proteins / Q000235:genetics* / Q000378:metabolism; D019216:Metals, Heavy / Q000633:toxicity; D008954:Models, Biological; D005810:Multigene Family; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics; D010269:Paraquat / Q000633:toxicity; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics; D036226:Seedlings / Q000187:drug effects / Q000235:genetics; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*
chemical_list: D007506:Iron-Sulfur Proteins; D019216:Metals, Heavy; D010269:Paraquat
keywords: Fe-S cluster assembly genes; iron homeostasis; rice