title: Desflurane accelerates neuronal cytotoxicity of Aβ by downregulating miR-214.
abstract: Since more and more evidence suggests anesthetics serve as a risk factor of Alzheimer's disease (AD), we examine the neurotoxicity of a commonly used inhalational anesthetics, desflurane, and its potential toxicity mechanism in primary rat hippocampal neurons. Here we show that desflurane increase the cytotoxicity of intracellular and extracellular amyloid β (Aβ) in the presence or absence of serum. It is also demonstrated that the cytotoxicity of desflurane is caused by the reduction of miR-214 which binds to Bax 3'UTR and results in the increased expression of Bax. Therefore, we conclude that desflurane accelerates neuronal cytotoxicity of Aβ by downregulating miR-214. Our study sheds a light on the therapy of cytotoxicity induced by inhaled anesthetics, especially in patients of AD.
authors.affiliation: State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: You
authors.initials: Y
authors.lastname: Yu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D016229:Amyloid beta-Peptides / Q000378:metabolism* / Q000633:toxicity; D018685:Anesthetics, Inhalation / Q000633:toxicity*; D000818:Animals; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D000077335:Desflurane; D015536:Down-Regulation; D006624:Hippocampus / Q000166:cytology; D007530:Isoflurane / Q000031:analogs & derivatives* / Q000633:toxicity; D035683:MicroRNAs / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D010446:Peptide Fragments / Q000378:metabolism* / Q000633:toxicity; D017207:Rats, Sprague-Dawley; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D018685:Anesthetics, Inhalation; D016895:Culture Media, Serum-Free; D035683:MicroRNAs; C559947:Mirn214 microRNA, rat; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D051028:bcl-2-Associated X Protein; D000077335:Desflurane; D007530:Isoflurane
keywords: Alzheimer's disease; Bax; Cell death; Desflurane; miR-214