title: Virulence factors and antimicrobial resistance of escherichia coli isolated from urinary tract of swine in southern of Brazil.
abstract: The present study determined the molecular and resistance patterns of E. coli isolates from urinary tract of swine in Southern of Brazil. Molecular characterization of urinary vesicle samples was performed by PCR detection of virulence factors from ETEC, STEC and UPEC. From a total of 82 E. coli isolates, 34 (38.63%) harbored one or more virulence factors. The frequency of virulence factors genes detected by PCR were: pap (10.97%), hlyA (10.97%), iha (9.75%), lt (8.53%), sta (7.31%) sfa (6.09%), f4 (4.87%), f5 (4.87%), stb (4.87%), f6 (1.21%) and f41 (1.21%). Isolates were resistant to penicillin (95.12%), lincomycin (93.9%), erythromycin (92.68%), tetracycline (90.24%), amoxicillin (82.92%), ampicillin (74.39%), josamycin (79.26%), norfloxacin (58.53%), enrofloxacin (57.31%), gentamicin (39.02%), neomycin (37.8%), apramycin (30.48%), colistine (30.48%) and cefalexin (6.09%). A number of 32 (39.02%) E. coli isolates harbored plasmids.
authors.affiliation: Departamento de Biologia Molecular e Biotecnologia, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul , Porto Alegre, RS , Brasil ; Faculdade de Medicina Veterinária, Universidade do Oeste de Santa Catarina , Xanxerê, SC , Brasil.
authors.forename: Mateus Matiuzzi
authors.initials: MM
authors.lastname: da Costa
authors.forename: Guilherme
authors.initials: G
authors.lastname: Drescher
authors.forename: Franciele
authors.initials: F
authors.lastname: Maboni
authors.forename: Shana
authors.initials: S
authors.lastname: Weber
authors.forename: Sônia
authors.initials: S
authors.lastname: de Avila Botton
authors.forename: Marilene Henning
authors.initials: MH
authors.lastname: Vainstein
authors.forename: Irene Silveira
authors.initials: IS
authors.lastname: Schrank
authors.forename: Agueda Castagna
authors.initials: AC
authors.lastname: de Vargas
keywords: E. coli; antimicrobial resistance; plasmids; swine; virulence factors
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