title: 'Switch-off' biosensing for chymotrypsin-catalyzed reaction by SPR-SERS spectroscopy.
abstract: We describe a novel 'switch-off' biosensing strategy for the detection of chymotrypsin based on surface plasmon resonance (SPR) and surface-enhanced Raman scattering (SERS) spectroscopy. This approach analyzes the fingerprint spectrum of the chymotrypsin-catalyzed substrate. The lowest probed concentration is 0.4 nM for chymotrypsin.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, 2699 Qianjin Ave., Changchun 130012, P. R. China. xusp@jlu.edu.cn.
authors.forename: Cuicui
authors.initials: C
authors.lastname: Fu
authors.forename: Weiqing
authors.initials: W
authors.lastname: Xu
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Shuping
authors.initials: S
authors.lastname: Xu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002918:Chymotrypsin / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D002918:Chymotrypsin