title: Structural analysis of RNA backbone using in-line probing.
abstract: To determine the secondary structure characteristics of nucleotides in an RNA polymer.
authors.affiliation: Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Ali
authors.initials: A
authors.lastname: Nahvi
authors.forename: Rachel
authors.initials: R
authors.lastname: Green
mesh_terms: D000107:Acetylation; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry* / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D012313:RNA
keywords: In vitro RNA transcription; In-line probing; Labeling RNA; Polyacrylamide gel electrophoresis (PAGE); Selective 2′-hydroxyl acetylation and primer extension (SHAPE)