title: Grapevine NAC1 transcription factor as a convergent node in developmental processes, abiotic stresses, and necrotrophic/biotrophic pathogen tolerance.
abstract: Transcription factors of the NAC family are known to be involved in various developmental processes and in response to environmental stresses. Whereas NAC genes have been widely studied in response to abiotic stresses, little is known about their role in response to biotic stresses, especially in crops. Here, the first characterization of a Vitis vinifera L. NAC member, named VvNAC1, and involved in organ development and defence towards pathogens is reported. Expression profile analysis of VvNAC1 showed that its expression is closely associated with later stages of leaf, flower, and berry development, suggesting a role in plant senescence. Moreover, VvNAC1 expression is stimulated in Botrytis cinerea- or microbe-associated molecular pattern (MAMP)-infected berries or leaves. Furthermore, cold, wounding, and defence-related hormones such as salicylic acid, methyl jasmonate, ethylene, and abscisic acid are all able to induce VvNAC1 expression in grapevine leaves. VvNAC1-overexpressing Arabidopsis plants exhibit enhanced tolerance to osmotic, salt, and cold stresses and to B. cinerea and Hyaloperonospora arabidopsidis pathogens. These plants present a modified pattern of defence gene markers (AtPR-1, AtPDF1.2, and AtVSP1) after stress application, suggesting that VvNAC1 is an important regulatory component of the plant signalling defence cascade. Collectively, these results provide evidence that VvNAC1 could represent a node of convergence regulating grapevine development and stress responses, including defence against necrotrophic and biotrophic pathogens.
authors.affiliation: Université de Reims Champagne-Ardenne, URVVC-EA 4707, Laboratoire Stress, Défenses et Reproduction des Plantes, BP 1039, F-51687 Reims cedex 2, France.
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Le Hénanff
authors.forename: Camille
authors.initials: C
authors.lastname: Profizi
authors.forename: Barbara
authors.initials: B
authors.lastname: Courteaux
authors.forename: Fanja
authors.initials: F
authors.lastname: Rabenoelina
authors.forename: Clémentine
authors.initials: C
authors.lastname: Gérard
authors.forename: Christophe
authors.initials: C
authors.lastname: Clément
authors.forename: Fabienne
authors.initials: F
authors.lastname: Baillieul
authors.forename: Sylvain
authors.initials: S
authors.lastname: Cordelier
authors.forename: Sandrine
authors.initials: S
authors.lastname: Dhondt-Cordelier
mesh_terms: D020171:Botrytis / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020156:Salicylic Acid / Q000378:metabolism; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D027843:Vitis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism / Q000382:microbiology*
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D020156:Salicylic Acid
keywords: Abiotic stress; Botrytis cinerea; Hyaloperonospora arabidopsidis; VvNAC1.; biotic stress; development; grapevine; transcription factor