title: Optimization of nutritional requirements for mycelial growth and sporulation of entomogenous fungus Aschersonia aleyrodis Webber.
abstract: The objective of the present study was to investigate the optimal nutritional requirements for mycelial growth and sporulation of entomopathogenic fungus Aschersonia aleyrodis Webber by orthogonal layout methods. Herein the order of effects of nutrient components on mycelial growth was tryptone > Ca(2+) > soluble starch > folacin, corresponding to the following optimal concentrations: 1.58% Soluble Starch, 3.16% Tryptone, 0.2 mmol l(-1) Ca(2+) and 0.005% Folacin. The optimal concentration of each factors for sporulation was 1.16% lactose, 0.394% tryptone, 0.4 mmol l(-1) Fe(2+) and 0.00125% VB1, and the effects of medium components on sporulation were found to be in the order lactose > VB1 > Fe(2+) > tryptone. Under the optimal culture conditions, the maximum production of mycelial growth achieved 20.05 g l(-1) after 7 days of fermentation, while the maximum spore yield reached 5.23 ×10(10) spores l(-1) after 22 days of cultivation. This is the first report on optimization of nutritional requirements and design of simplified semi-synthetic media for mycelial growth and sporulation of A. aleyrodis.
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University , Fuzhou 350002 , China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhu
authors.forename: Jieru
authors.initials: J
authors.lastname: Pan
authors.forename: Junzhi
authors.initials: J
authors.lastname: Qiu
authors.forename: Xiong
authors.initials: X
authors.lastname: Guan
keywords: Aschersonia aleyrodis; Entomogenous fungus; Mycelial Growth; Orthogonal matrix method; Sporulation
references.citation: J Appl Microbiol. 2004;96(5):1105-16
references.pmid: 15078528
references.citation: J Appl Microbiol. 2005;99(3):483-92
references.pmid: 16108789