title: Development and characterization of hybrids from native wine yeasts.
abstract: For commercial purposes, the winemaking industry is constantly searching for new yeast strains. Historically, this has been achieved by collecting wild strains and selecting the best for industrial use through an enological evaluation. Furthermore, the increasing consumer demands have forced the industry to incorporate new strategies such as genetic engineering to obtain improved strains. In response to the lack of public acceptance of this methodology, alternative strategies based on breeding have gained acceptance in recent years. Through the use of conjugation of individual spores without the support of genetic engineering methods we generated intraspecific hybrids from wild strains with outstanding enological characteristics and interdelta fingerprinting was used to confirm the hybrid condition. A detailed enological characterization of the hybrids in synthetic and natural must indicates that physiological parameters such as sporulation, residual sugar, ethanol yield and total nitrogen uptake are within the levels determined for the parental strains, however, other parameters such as growth rate, lag phase and ethanol production show statistical differences with some parental or commercial strains. These findings allow us to propose these hybrids as new wine-making strains.
authors.affiliation: Departamento de Ciencia y Tecnología de los Alimentos, Universidad de Santiago de Chile , USACH, Alameda 3363, Estación Central, Santiago , Chile.
authors.forename: Verónica
authors.initials: V
authors.lastname: García
authors.forename: José
authors.initials: J
authors.lastname: Rivera
authors.forename: Angela
authors.initials: A
authors.lastname: Contreras
authors.forename: María Angélica
authors.initials: MA
authors.lastname: Ganga
authors.forename: Claudio
authors.initials: C
authors.lastname: Martínez
keywords: Hybrids; Wine; Yeast
references.citation: Appl Environ Microbiol. 1985 Oct;50(4):1064-7
references.pmid: 16346903
references.citation: J Appl Microbiol. 2007 Sep;103(3):735-42
references.pmid: 17714407
references.citation: FEMS Yeast Res. 2006 Mar;6(2):268-79
references.pmid: 16487348
references.citation: Lett Appl Microbiol. 2005;41(2):147-52
references.pmid: 16033512
references.citation: Genetics. 1998 Mar;148(3):923-8
references.pmid: 9539413
references.citation: Appl Microbiol Biotechnol. 2005 Mar;66(6):622-8
references.pmid: 15578179
references.citation: Yeast. 2000 Jun 15;16(8):675-729
references.pmid: 10861899
references.citation: J Biosci Bioeng. 2009 Apr;107(4):383-93
references.pmid: 19332297
references.citation: Cell. 1984 May;37(1):67-75
references.pmid: 6373014
references.citation: Yeast. 1997 Jul;13(9):783-93
references.pmid: 9234667
references.citation: Bioresour Technol. 2011 Feb;102(3):3020-7
references.pmid: 20980141
references.citation: Microbiology (Reading). 1997 Jan;143 ( Pt 1):203-218
references.pmid: 9025295
references.citation: J Appl Microbiol. 2005;98(4):971-9
references.pmid: 15752344
references.citation: Food Microbiol. 2006 Oct;23(7):672-6
references.pmid: 16943067
references.citation: J Appl Microbiol. 2004;96(5):1161-8
references.pmid: 15078534
references.citation: Nucleic Acids Res. 1995 Nov 11;23(21):4407-14
references.pmid: 7501463
references.citation: J Biosci Bioeng. 2004;98(5):353-8
references.pmid: 16233719
references.citation: Appl Environ Microbiol. 1992 Sep;58(9):2948-53
references.pmid: 16348768
references.citation: Appl Microbiol Biotechnol. 2009 Oct;84(5):937-54
references.pmid: 19711068
references.citation: J Appl Microbiol. 2006 Mar;100(3):516-26
references.pmid: 16478491
references.citation: Appl Environ Microbiol. 2004 Jun;70(6):3392-400
references.pmid: 15184136
references.citation: Res Microbiol. 2000 Sep;151(7):583-90
references.pmid: 11037136
references.citation: J Appl Microbiol. 2010 May;108(5):1850-8
references.pmid: 20163487
references.citation: FEMS Yeast Res. 2007 Sep;7(6):953-65
references.pmid: 17484739
references.citation: FEMS Microbiol Lett. 2003 Apr 25;221(2):249-55
references.pmid: 12725935
references.citation: FEMS Yeast Res. 2004 May;4(7):711-9
references.pmid: 15093774
references.citation: Appl Environ Microbiol. 1998 Dec;64(12):5039-41
references.pmid: 9835605
references.citation: FEMS Yeast Res. 2009 Dec;9(8):1148-60
references.pmid: 19758333