title: Incorporation of nisin in natural casing for the control of spoilage microorganisms in vacuum packaged sausage.
abstract: This study aimed to evaluate the effectiveness of natural casing treatment with nisin and phosphoric acid on control of spoilage microorganisms in vacuum packaged sausages. Ovine casings were dipped in the following baths: 1) 0.1% food grade phosphoric acid; 2) 5.0 mg/L nisin; 3) 0.1% phosphoric acid and 5.0 mg/L nisin; and 4) sterile water (control). The sausages were produced in a pilot plant, stuffed into the pretreated natural casings, vacuum packaged and stored at 4 and 10 °C for 56 days. The experiments were performed according to a full factorial design 2(3), totalizing 8 treatments that were repeated in 3 blocks. Aerobic plate counts and lactic acid bacteria analysis were conducted at 1, 14, 28, 42 and 56 days of storage. Treatment of casings with phosphoric acid 0.1% alone did not inhibit the growth of lactic acid bacteria and reduced the aerobic plate count by 1 log. The activity of nisin against lactic acid bacteria was enhanced by the addition of phosphoric acid, demonstrating a synergistic effect. Furthermore nisin activity was more evident at lower storage temperature (4 ºC). Therefore treatment of the natural casings with nisin and phosphoric acid, combined with low storage temperature, are obstacles that present a potential for controlling the growth of lactic acid bacteria in vacuum packaged sausage.
authors.affiliation: Faculdade de Tecnologia Termomecanica , São Bernardo do Campo, SP , Brasil.
authors.forename: Joyce Regina
authors.initials: JR
authors.lastname: de Barros
authors.forename: Leo
authors.initials: L
authors.lastname: Kunigk
authors.forename: Cynthia Hyppolito
authors.initials: CH
authors.lastname: Jurkiewicz
keywords: Bacteriocin; Natural casing; Nisin; Sausage
references.citation: Nat Rev Microbiol. 2005 Oct;3(10):777-88
references.pmid: 16205711
references.citation: Meat Sci. 2000 Oct;56(2):105-15
references.pmid: 22061897
references.citation: J Food Prot. 2002 Nov;65(11):1780-3
references.pmid: 12430703
references.citation: Food Microbiol. 2008 Oct;25(7):866-70
references.pmid: 18721674
references.citation: Poult Sci. 2002 May;81(5):721-6
references.pmid: 12033424
references.citation: J Food Prot. 2005 Apr;68(4):744-50
references.pmid: 15830665
references.citation: J Food Prot. 1999 Sep;62(9):1004-10
references.pmid: 10492474
references.citation: Meat Sci. 2008 Jan;78(1-2):53-9
references.pmid: 22062095
references.citation: Lett Appl Microbiol. 2005;41(6):464-9
references.pmid: 16305671
references.citation: J Food Prot. 2000 Oct;63(10):1338-46
references.pmid: 11041132
references.citation: J Food Prot. 1999 Aug;62(8):913-20
references.pmid: 10456746
references.citation: J Food Prot. 2007 Apr;70(4):1017-20
references.pmid: 17477277
references.citation: Food Addit Contam. 2005 Oct;22(10):980-7
references.pmid: 16227182
references.citation: J Appl Microbiol. 2005;98(6):1316-25
references.pmid: 15916645
references.citation: Lett Appl Microbiol. 2005;40(2):106-10
references.pmid: 15644108
references.citation: J Food Prot. 2006 Jan;69(1):53-61
references.pmid: 16416901
references.citation: Lett Appl Microbiol. 1998 Jul;27(1):19-23
references.pmid: 9722992