title: Effect of xixin decoction on O-linked N-acetylglucosamine glycosylation of tau proteins in rat brain with sporadic Alzheimer disease.
abstract: OBJECTIVE
  To study the effects of Xixin decoction (XXD) on O-linked N-acetylglucosamine (O-GlcNAc) glycosylation of tau proteins in rat brain with sporadic Alzheimer disease (SAD), and discuss its possible mechanism on prevention and treatment of SAD.
  
  
  METHODS
  The rat model of SAD was established by intracerebroventricular injection of streptozotocin. The specific pathogen free male Sprague-Dawley rats were randomly divided into sham-operation group (S), model group (M), donepezil group (D), XXD at a low dose group (XL), XXD at a medium dose group (XM) and XXD at a high dose group (XH). After treatment and praxiology test, immunohistochemistry and western blotting were used to detect O-GlcNAc glycosylation level of tau proteins in rat brain with SAD. O-GlcNAc glycosylation and expression of tau proteins were detected by O-GlcNAc-specific antibodies RL2 and CTD110.6.
  
  
  RESULTS
  O-GlcNAc glycosylated proteins enriched by succinylated wheat germ agglutinin significantly improved in the hippocampus of SAD rats. The differences were statistically significant among XXD groups (P < 0.05, P < 0.01), while no obvious differences were observed between D group and M group (P > 0.05).
  
  
  CONCLUSIONS
  XXD can significantly improve O-GlcNAc glycosylation level of tau proteins in the hippocampus of SAD rats, which maybe inhibit hyperphosphorylation of tau proteins on key sites and its toxicity, and prevent the pathological process of SAD.
authors.affiliation: Shaanxi University of Chinese Medicine, Xianyang 712046, China. ycdw07@yahoo.com.cn
authors.forename: Yongchang
authors.initials: Y
authors.lastname: Diwu
authors.forename: Jinzhou
authors.initials: J
authors.lastname: Tian
authors.forename: Jing
authors.initials: J
authors.lastname: Shi
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism*; D000544:Alzheimer Disease / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006031:Glycosylation / Q000187:drug effects; D006801:Humans; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D016875:tau Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D016875:tau Proteins; D000117:Acetylglucosamine