title: [Study on the serotype, virulence genes and pulsed field gel electrophoresis molecular typing of Vibrio parahaemolyticus isolated from clinical patients].
abstract: OBJECTIVE
  To investigate the serotype virulence genes and molecular typing characteristics of Vibrio Parahaemolyticus isolated from diarrhea samples among 2007 -2012.
  
  
  METHODS
  The serotype was detected by using serological agglutination test. Thermo-stable direct hemolysin (tdh) and tdh-related hemolysin (trh) were detected by using real time PCR methods. 56 strains 03:K6 were analyzed for molecular typing by pulsed field gel electrophoresis(PFGE).
  
  
  RESULTS
  (1) There are 14 serotypes, including 03: K6 (114,67.9%),04: K8 (25,14.9%), 03: K29 (6,3.6%) and et al. Compared with the serotypes of strains from food poison, these from sporadic cases with diarrhea were different, but the main serotype was also 03:K6. (2) There are 4 types of virulence genes, including tdh+ trh- (161, 95.8%), tdh+ trh+ (3,1.8%), tdh- trh+ (1, 0.6%) tdh- trh- (3, 1.8%). Compared with the virulence genes of strains from food poison, these from sporadic cases with diarrhea were different, but the main virulence gene was also tdh+ trh-. 3,56 strains belonged to 15 PFGE strips and the similar degree is higher than 86%. 10 patterns (P1-P4, P6, P7, P9, P10, P12, P15) had at lest two strains.
  
  
  CONCLUSIONS
  The most V. Parahaemolyticus isolated from clinical patients was 03: K6 and tdh+ trh-. PFGE strips of V. Parahaemolyticus of 03: K6 from clinical patients exhibited a high degree of similarity, and correlation was found among strains.
authors.affiliation: Center for Disease Control and Prevention of Futian District Shenzhen City, Shenzhen 518040, China. rainingdot2010@163.com
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhifa
authors.initials: Z
authors.lastname: Lai
authors.forename: Haitao
authors.initials: H
authors.lastname: Zhou
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Runli
authors.initials: R
authors.lastname: Chen
authors.forename: Huashu
authors.initials: H
authors.lastname: Zeng
authors.forename: Hongbin
authors.initials: H
authors.lastname: Hou
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D002681:China; D003967:Diarrhea / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005517:Foodborne Diseases / Q000382:microbiology*; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D058889:Molecular Typing / Q000379:methods*; D012703:Serotyping; D014736:Vibrio parahaemolyticus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014774:Virulence / Q000235:genetics; D055815:Young Adult