title: Antimicrobial Effect of Jasminum grandiflorum L. and Hibiscus rosa-sinensis L. Extracts Against Pathogenic Oral Microorganisms--An In Vitro Comparative Study.
abstract: OBJECTIVE
  To assess and compare the antimicrobial potential and determine the minimum inhibitory concentration (MIC) of Jasminum grandiflorum and Hibiscus rosa-sinensis extracts as potential anti-pathogenic agents in dental caries.
  
  
  METHODS
  Aqueous and ethanol (cold and hot) extracts prepared from leaves of Jasminum grandiflorum and Hibiscus rosa-sinensis were screened for in vitro antimicrobial activity against Streptococcus mutans and Lactobacillus acidophilus using the agar well diffusion method. The lowest concentration of every extract considered as the minimum inhibitory concentration (MIC) was determined for both test organisms. Statistical analysis was performed with one-way analysis of variance (ANOVA).
  
  
  RESULTS
  At lower concentrations, hot ethanol Jasminum grandiflorum (10 μg/ml) and Hibiscus rosa-sinensis (25 μg/ml) extracts were found to have statistically significant (P≤0.05) antimicrobial activity against S. mutans and L. acidophilus with MIC values of 6.25 μg/ml and 25 μg/ml, respectively. A proportional increase in their antimicrobial activity (zone of inhibition) was observed.
  
  
  CONCLUSIONS
  Both extracts were found to be antimicrobially active and contain compounds with therapeutic potential. Nevertheless, clinical trials on the effect of these plants are essential before advocating large-scale therapy.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Nagarajappa
authors.forename: Mehak
authors.initials: M
authors.lastname: Batra
authors.forename: Archana J
authors.initials: AJ
authors.lastname: Sharda
authors.forename: Kailash
authors.initials: K
authors.lastname: Asawa
authors.forename: Sudhanshu
authors.initials: S
authors.lastname: Sanadhya
authors.forename: Hemasha
authors.initials: H
authors.lastname: Daryani
authors.forename: Gayathri
authors.initials: G
authors.lastname: Ramesh
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003731:Dental Caries / Q000382:microbiology; D000431:Ethanol / Q000737:chemistry; D031584:Hibiscus / Q000737:chemistry; D006801:Humans; D031660:Jasminum / Q000737:chemistry; D007779:Lactobacillus acidophilus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D012997:Solvents / Q000737:chemistry; D013295:Streptococcus mutans / Q000187:drug effects*; D014867:Water / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D012997:Solvents; D014867:Water; D000431:Ethanol