title: Polysome analysis of mammalian cells.
abstract: To assess the global translational level of mammalian cells (see similar protocols for bacteria and yeast on Analysis of polysomes from bacteria, Polysome Profile Analysis - Yeast and Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae).
authors.affiliation: Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Shan L
authors.initials: SL
authors.lastname: He
authors.forename: Rachel
authors.initials: R
authors.lastname: Green
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D016678:Genome; D006801:Humans; D011132:Polyribosomes / Q000737:chemistry / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012270:Ribosomes / Q000235:genetics
chemical_list: D012333:RNA, Messenger
keywords: Cell lysate; Genome-wide analysis techniques; Immobilize ribosomes; Mammalian cells; Polysome analysis; Size fractionation; Sucrose density gradient centrifugation