title: Bacillus sediminis sp. nov., isolated from an electroactive biofilm.
abstract: A Gram-stain positive, facultative anaerobic, motile, spore-forming rod-shaped bacterium with peritrichous flagella, designated DX-5(T), was isolated from an electroactive biofilm. Growth was observed to occur at 35-60 °C, at pH 7.0-10.0 and with 0.5-10 % (w/v) NaCl (optimum growth: 50 °C, pH 8.0 and 0.5-3 % NaCl). Cells were determined to be catalase- and oxidase-positive. The predominant respiratory quinone was identified as MK-7; the major polar lipids were determined to be diphosphatidylglycerol, phosphatidylglycerol, glycolipid, aminoglycolipid and an unidentified phospholipid; the DNA G+C content was determined to be 46.6 mol%; and the major fatty acids (>5 %) were identified as anteiso-C15:0 (33.6 %), iso-C15:0 (24.1 %) and anteiso-C17:0 (13.4 %). The phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain DX-5(T) should be assigned to the genus Bacillus, and was related most closely to the type strains of B. fortis DSM 16012(T) (96.3 %), B. composti KACC 16872(T) (96.3 %) and B. fordii DSM 16014(T) (95.8 %). Results of phenotypic, chemotaxonomic and genotypic analysis indicated that strain DX-5(T) represents a novel species, for which the name B. sediminis sp. nov. is proposed. The type strain is DX-5(T) (=CGMCC 1.12412(T) = KCTC 33102(T)).
authors.affiliation: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou, 510650, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yu
authors.forename: Yueqiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Dongxing
authors.initials: D
authors.lastname: Qin
authors.forename: Guiqin
authors.initials: G
authors.lastname: Yang
authors.forename: Shungui
authors.initials: S
authors.lastname: Zhou
mesh_terms: D000332:Aerobiosis; D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D018441:Biofilms; D002374:Catalase / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004560:Electricity; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008124:Locomotion; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013171:Spores, Bacterial / Q000166:cytology; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D010088:Oxidoreductases; D002374:Catalase