title: Maximizing recovery of water-soluble proteins through acetone precipitation.
abstract: Solvent precipitation is commonly used to purify protein samples, as seen with the removal of sodium dodecyl sulfate through acetone precipitation. However, in its current practice, protein loss is believed to be an inevitable consequence of acetone precipitation. We herein provide an in depth characterization of protein recovery through acetone precipitation. In 80% acetone, the precipitation efficiency for six of 10 protein standards was poor (ca. ≤15%). Poor recovery was also observed for proteome extracts, including bacterial and mammalian cells. As shown in this work, increasing the ionic strength of the solution dramatically improves the precipitation efficiency of individual proteins, and proteome mixtures (ca. 80-100% yield). This is obtained by including 1-30 mM NaCl, together with acetone (50-80%) which maximizes protein precipitation efficiency. The amount of salt required to restore the recovery correlates with the amount of protein in the sample, as well as the intrinsic protein charge, and the dielectric strength of the solution. This synergistic approach to protein precipitation in acetone with salt is consistent with a model of ion pairing in organic solvent, and establishes an improved method to recover proteins and proteome mixtures in high yield.
authors.affiliation: Department of Chemistry, Dalhousie University, Halifax, Nova Scotia, Canada.
authors.forename: Andrew M J
authors.initials: AM
authors.lastname: Crowell
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Wall
authors.forename: Alan A
authors.initials: AA
authors.lastname: Doucette
mesh_terms: D000096:Acetone / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D011232:Chemical Precipitation; D004926:Escherichia coli / Q000737:chemistry; D029968:Escherichia coli Proteins / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry; D009994:Osmolar Concentration; D011506:Proteins / Q000737:chemistry*; D012492:Salts / Q000737:chemistry; D012995:Solubility; D014867:Water / Q000737:chemistry; D015003:Yeasts / Q000737:chemistry
chemical_list: D029968:Escherichia coli Proteins; D005656:Fungal Proteins; D011506:Proteins; D012492:Salts; D014867:Water; D000096:Acetone
keywords: Acetone; Ion pairing; Ionic strength; Protein precipitation; Protein recovery; Salt