title: Myosin VI contributes to maintaining epithelial barrier function.
abstract: BACKGROUND
  Epithelial barrier dysfunction is associated with the pathogenesis of a number of immune inflammations; the etiology is not fully understood. The fusion of endosome/lysosome is a critical process in the degradation of endocytic antigens in epithelial cells. Recent reports indicate that myosin VI (myo6) is involved in the activities of endosomes. The present study aims to investigate the role of myo6 in epithelial barrier dysfunction.
  
  
  RESULTS
  The endosome accumulation was observed in myo6-deficient Rmcs. More than 80% endosomes were fused with lysosomes in naïve Rmcs while less than 30% endosomes were fused with lysosomes in the myo6-deficient Rmcs. The myo6-deficient Rmc monolayers showed high permeability to a macromolecular antigen, ovalbumin, the latter still conserved the antigenicity, which induced strong T cell activation.
  
  
  CONCLUSIONS
  We conclude that myo6 plays a critical role in the fusion of endosome/lysosome in Rmc epithelial cells. Deficiency of myo6 compromises the epithelial barrier function.
authors.affiliation: Department of Anesthesiology, Shengjing Hospital, China Medical University, 36 Sanhao St, Shenyang 110004, China. Wuxm1@sj-hospital.org.
authors.forename: Yu-Wei
authors.initials: YW
authors.lastname: Liao
authors.forename: Xing-Mao
authors.initials: XM
authors.lastname: Wu
authors.forename: Jia
authors.initials: J
authors.lastname: Jia
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Wu
authors.forename: Tao
authors.initials: T
authors.lastname: Hong
authors.forename: Ling-Xin
authors.initials: LX
authors.lastname: Meng
authors.forename: Xiu-Ying
authors.initials: XY
authors.lastname: Wu
mesh_terms: D000818:Animals; D002460:Cell Line; D011992:Endosomes / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D008247:Lysosomes / Q000378:metabolism*; D051379:Mice; D018995:Myosin Heavy Chains / Q000235:genetics* / Q000378:metabolism
chemical_list: C089785:myosin VI; D018995:Myosin Heavy Chains
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