title: In vivo response of some immune and endocrine variables to LPS in Eurasian perch (Perca fluviatilis, L.) and modulation of this response by two corticosteroids, cortisol and 11-deoxycorticosterone.
abstract: In fish, the endocrine system, especially corticosteroids pathway, strongly interacts with immune system. On the other hand, in vivo co-stimulation of both systems is not well documented. To better understand this interaction, we decided to evaluate the in vivo effects of both stimulation of the immune system and co-stimulation of both systems in Eurasian perch juveniles. Fish were injected either with 10mgkg(-1) LPS, or with a combination of LPS and 0.8mgkg(-1) cortisol or LPS and 0.08mgkg(-1) 11-deoxycorticosterone (DOC) and sampled 1, 3 or 7days after injection. LPS affected the immune system by increasing plasma lysozyme activity and blood neutrophils populations. During the same time-course, LPS decreased the proportion of a mixture of lymphocytes and thrombocytes in blood and TNF-α expression in spleen. Cortisol modulated the LPS-mediated response in TNF-α mRNA expression levels in spleen. Contrary to LPS alone, the association of LPS with DOC modulated the abundance of complement component 3 (C3) mRNA in spleen. On the other hand, LPS altered the corticotropic axis by decreasing mRNA expression levels of all corticosteroid receptors and of 11β-HSD-2 in spleen. Both corticosteroids injected were not able to balance these LPS-induced suppressive effects on corticosteroid receptors and 11β-HSD-2 expression levels in spleen. Contrary to LPS alone, the association of LPS with DOC modulated GR-1b expression in gills. These results indicated that LPS is a strong modulator of the corticosteroid receptors expression in spleen. Furthermore, we report for the first time a LPS-induced decrease of the mineralocorticoid receptor expression. Finally, corticosteroids were able to modulate the LPS-mediated response at the transcriptional level.
authors.affiliation: University of Namur (FUNDP), Research Unit in Environmental and Evolutionary Biology (URBE), Rue de Bruxelles 61, B-5000 Namur, Belgium. Electronic address: cedric.mathieu@unamur.be.
authors.forename: Cédric
authors.initials: C
authors.lastname: Mathieu
authors.forename: Sylvain
authors.initials: S
authors.lastname: Milla
authors.forename: S N M
authors.initials: SN
authors.lastname: Mandiki
authors.forename: Jessica
authors.initials: J
authors.lastname: Douxfils
authors.forename: Patrick
authors.initials: P
authors.lastname: Kestemont
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000276:immunology; D003176:Complement C3 / Q000235:genetics / Q000378:metabolism; D003900:Desoxycorticosterone / Q000494:pharmacology / Q000502:physiology*; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000276:immunology; D006854:Hydrocortisone / Q000494:pharmacology / Q000502:physiology*; D007155:Immunologic Factors / Q000494:pharmacology / Q000502:physiology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008214:Lymphocytes / Q000276:immunology; D009113:Muramidase / Q000097:blood; D009504:Neutrophils / Q000276:immunology; D010471:Perches / Q000276:immunology* / Q000378:metabolism; D011987:Receptors, Steroid / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D003176:Complement C3; D029941:Fish Proteins; D007155:Immunologic Factors; D008070:Lipopolysaccharides; D011987:Receptors, Steroid; D014409:Tumor Necrosis Factor-alpha; D003900:Desoxycorticosterone; D009113:Muramidase; D006854:Hydrocortisone
keywords: 11-Deoxycorticosterone (DOC); Corticosteroid receptors; Cortisol; Fish; Immunity; Intraperitoneal injection; Lipopolysaccharide (LPS); Perca fluviatilis