title: Detection of enterotoxin genes of Staphylococcus SP isolated from nasal cavities and hands of food handlers.
abstract: Food handlers, an important factor in food quality, may contain bacteria that are able to cause foodborne disease. The present study aimed to research coagulase-negative (CNS) and -positive staphylococci (CPS) in 82 food handlers, analyzing nasal and hand swabs, with identification of 62 CNS (75.6%) and 20 CPS strains (24.4%). Staphylococcal enterotoxins genes were investigated by PCR. In 20 CPS strains, 19 were positive for one or more genes. The percentage of CNS presenting genes for enterotoxins was high (46.8%). Despite of the staphylococcal species, the most common gene was sea (35.4%), followed by seh and sej (29.2%). The detection of new staphylococcal enterotoxins (SEs) genes showed a higher pathogenic potential in this genus. The presence of these gene points out the importance of CNS not only as contaminant bacteria but also as a pathogen.
authors.affiliation: Departamento de Microbiologia e Imunologia, Instituto de Biociências, Universidade Estadual Paulista , Botucatu, SP , Brasil.
authors.forename: V L M
authors.initials: VL
authors.lastname: Rall
authors.forename: J M
authors.initials: JM
authors.lastname: Sforcin
authors.forename: V C M
authors.initials: VC
authors.lastname: Augustini
authors.forename: M T
authors.initials: MT
authors.lastname: Watanabe
authors.forename: A
authors.initials: A
authors.lastname: Fernandes
authors.forename: R
authors.initials: R
authors.lastname: Rall
authors.forename: M G
authors.initials: MG
authors.lastname: Silva
authors.forename: J P
authors.initials: JP
authors.lastname: Araújo
keywords: S. aureus; Staphylococcal enterotoxins; coagulase-negative staphylococci; food handler
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