title: IL-22 secreted by decidual stromal cells and NK cells promotes the survival of human trophoblasts.
abstract: Interleukin-22 (IL-22) has been implicated as an important immune regulator in many physiologic and pathological processes, but little is known about the IL-22 in the fetal-maternal interface. In this study, we demonstrated that co-culture of decidual stromal cells (DSCs) and decidual natural killer (dNK) cells resulted in increased secretion of IL-22, compared to culture of DSCs or dNK cells alone. The trophoblast cell line, HTR8/SVneo, expresses IL-22 receptor α1 (IL-22R1). Combinant human (rh) IL-22 significantly promoted the proliferation and viability, and inhibited the apoptosis of HTR8/SVneo cells. By Western blotting and immunohistochemistry, we confirmed that villi expressed IL-22R1, and the villi from unexplained spontaneous miscarriage patients expressed reduced levels of IL-22R1 than those from normal early pregnancy. These findings indicate that the IL-22 secreted by DSCs and dNK might promote the survival of trophoblasts and participate in the maintenance of pregnancy by binding to the IL-22R1. The reduced level of IL-22/IL-22R1 in villi might be involved in the occurrence of spontaneous miscarriage.
authors.affiliation: Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College, Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Shanghai 200011, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
authors.forename: Ming-Qing
authors.initials: MQ
authors.lastname: Li
authors.forename: Da-Jin
authors.initials: DJ
authors.lastname: Li
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Jin
mesh_terms: D000022:Abortion, Spontaneous / Q000378:metabolism; D017209:Apoptosis; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D018920:Coculture Techniques; D003656:Decidua / Q000378:metabolism*; D005260:Female; D006801:Humans; D007378:Interleukins / Q000378:metabolism*; D007694:Killer Cells, Natural / Q000378:metabolism*; D019899:Paracrine Communication; D011247:Pregnancy; D018123:Receptors, Interleukin / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D015398:Signal Transduction; D017154:Stromal Cells / Q000378:metabolism*; D014327:Trophoblasts / Q000378:metabolism*; D000097043:Interleukin-22
chemical_list: D007378:Interleukins; D018123:Receptors, Interleukin; D011994:Recombinant Proteins; D000097043:Interleukin-22; C415369:interleukin-22 receptor
keywords: HTR8/SVneo cells; IL-22; NK cells; decidual stromal cells; miscarriage
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