title: Interleukin-1β triggers the differentiation of macrophages with enhanced capacity to present mycobacterial antigen to T cells.
abstract: The rapid differentiation of monocytes into macrophages (MΦ) and dendritic cells is a pivotal aspect of the innate immune response. Differentiation is triggered following recognition of microbial ligands that activate pattern recognition receptors or directly by pro-inflammatory cytokines. We demonstrate that interleukin-1β (IL-1β) induces the rapid differentiation of monocytes into CD209(+) MΦ, similar to activation via Toll-like receptor 2/1, but with distinct phenotypic and functional characteristics. The IL-1β induced MΦ express higher levels of key markers of phagocytosis, including the Fc-receptors CD16 and CD64, as well as CD36, CD163 and CD206. In addition, IL-1β-induced MΦ exert potent phagocytic activity towards inert particles, oxidized low-density lipoprotein and mycobacteria. Furthermore, IL-1β-induced MΦ express higher levels of HLA-DR and effectively present mycobacterial antigens to T cells. Therefore, the ability of IL-1β to induce monocyte differentiation into MΦ with both phagocytosis and antigen-presenting function is a distinct part of the innate immune response in host defence against microbial infection.
authors.affiliation: Division of Dermatology, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
authors.forename: Mirjam
authors.initials: M
authors.lastname: Schenk
authors.forename: Mario
authors.initials: M
authors.lastname: Fabri
authors.forename: Stephan R
authors.initials: SR
authors.lastname: Krutzik
authors.forename: Delphine J
authors.initials: DJ
authors.lastname: Lee
authors.forename: David M
authors.initials: DM
authors.lastname: Vu
authors.forename: Peter A
authors.initials: PA
authors.lastname: Sieling
authors.forename: Dennis
authors.initials: D
authors.lastname: Montoya
authors.forename: Philip T
authors.initials: PT
authors.lastname: Liu
authors.forename: Robert L
authors.initials: RL
authors.lastname: Modlin
mesh_terms: D017951:Antigen Presentation; D000942:Antigens, Bacterial / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects*; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology*; D037181:Lectins, C-Type / Q000032:analysis; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D009000:Monocytes / Q000166:cytology; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010587:Phagocytosis; D011956:Receptors, Cell Surface / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology*; D051195:Toll-Like Receptor 2 / Q000502:physiology; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D000942:Antigens, Bacterial; D015815:Cell Adhesion Molecules; D053583:Interleukin-1beta; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D051195:Toll-Like Receptor 2; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C495342:TLR2 protein, human
keywords: antigen presentation; infection; innate immunity; macrophages/monocytes; mycobacteria/Mycobacterium
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