title: Development of a slide agglutination assay for detection of blastomycosis.
abstract: Blastomycosis, caused by the thermally dimorphic fungus Blastomyces dermatitides, which is endemic to eastern regions of the USA, is commonly misdiagnosed as a viral or bacterial infection and therefore treated improperly. Over the years, many immunodiagnostic assays to aid in the diagnosis of blastomycosis have been developed; however, a reliable assay for use in local clinics still remains elusive. Procedures for a slide agglutination assay for detection of antibody in serum from rabbits immunized with B. dermatitidis were evaluated with antigenic preparations from B. dermatitidis adsorbed to polystyrene microparticles. Yeast-phase lysates from five isolates of B. dermatitides: namely ER-593 (Eagle River, WI, USA), ER-598 (Eagle River, WI, USA), 48938 (India), B5896 (Mt. Iron, MN, USA), and T-58 (TN, USA) were evaluated for their sensitivity and specificity. Sensitivities of the lysates ranged from 29% to 83% whereas specificities ranged from 13% to 100%. Lysate ER-593 provided the most promising results with a sensitivity of 82% and specificity of 100%. This study provides suggests that a simple rapid slide agglutination assay for detecting blastomycosis may be used for screening patients with suspected B. dermatitidis infection.
authors.affiliation: Department of Biology, Idaho State University, Pocatello, Idaho, USA.
authors.forename: Wayne O
authors.initials: WO
authors.lastname: Hatch
authors.forename: Gene M
authors.initials: GM
authors.lastname: Scalarone
mesh_terms: D000372:Agglutination Tests / Q000295:instrumentation / Q000379:methods*; D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology; D001758:Blastomyces / Q000276:immunology / Q000302:isolation & purification*; D001759:Blastomycosis / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D006801:Humans; D011817:Rabbits; D012680:Sensitivity and Specificity
chemical_list: D000908:Antibodies, Fungal
keywords: Blastomyces dermatitidis; agglutination assay; blastomycosis