title: A direct and versatile assay measuring membrane penetration of adenovirus in single cells.
abstract: Endocytosis is the most prevalent entry port for viruses into cells, but viruses must escape from the lumen of endosomes to ensure that viral genomes reach a site for replication and progeny formation. Endosomal escape also helps viruses bypass endolysosomal degradation and presentation to certain Toll-like intrinsic immunity receptors. The mechanisms for cytosolic delivery of nonenveloped viruses or nucleocapsids from enveloped viruses are poorly understood, in part because no quantitative assays are readily available which directly measure the penetration of viruses into the cytosol. Following uptake by clathrin-mediated endocytosis or macropinocytosis, the nonenveloped adenoviruses penetrate from endosomes to the cytosol, and they traffic with cellular motors on microtubules to the nucleus for replication. In this report, we present a novel single-cell imaging assay which quantitatively measures individual cytosolic viruses and distinguishes them from endosomal viruses or viruses at the plasma membrane. Using this assay, we showed that the penetration of human adenoviruses of the species C and B occurs rapidly after virus uptake. Efficient penetration does not require acidic pH in endosomes. This assay is versatile and can be adapted to other adenoviruses and members of other nonenveloped and enveloped virus families.
authors.affiliation: Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.
authors.forename: Maarit
authors.initials: M
authors.lastname: Suomalainen
authors.forename: Stefania
authors.initials: S
authors.lastname: Luisoni
authors.forename: Karin
authors.initials: K
authors.lastname: Boucke
authors.forename: Sarah
authors.initials: S
authors.lastname: Bianchi
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Engel
authors.forename: Urs F
authors.initials: UF
authors.lastname: Greber
mesh_terms: D000256:Adenoviridae / Q000502:physiology*; D000257:Adenoviridae Infections / Q000378:metabolism / Q000821:virology*; D001681:Biological Assay; D036022:Capsid Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism / Q000821:virology*; D002966:Clathrin / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism / Q000821:virology; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism / Q000821:virology*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011991:Receptors, Virus / Q000378:metabolism; D053586:Virus Internalization
chemical_list: D036022:Capsid Proteins; D002966:Clathrin; D011991:Receptors, Virus; C090876:polypeptide VI, adenovirus
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