title: Comparison the effects of two monocyte isolation methods, plastic adherence and magnetic activated cell sorting methods, on phagocytic activity of generated dendritic cells.
abstract: OBJECTIVE
  It is believed that monocyte isolation methods and maturation factors affect the phenotypic and functional characteristics of resultant dendritic cells (DC). In the present study, we compared two monocyte isolation methods, including plastic adherence-dendritic cells (Adh-DC) and magnetic activated cell sorting- dendritic cells (MACS-DC), and their effects on phagocytic activity of differentiated immature DCs (immDCs).
  
  
  METHODS
  : In this experimental study, immDCs were generated from plastic adherence and MACS isolated monocytes in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4) in five days. The phagocytic activity of immDCs was analyzed by fluorescein isothiocyanate (FITC)-conjugated latex bead using flow cytometry. One way ANOVA test was used for statistical analysis of differences among experimental groups, including Adh-DC and MACS-DC groups.
  
  
  RESULTS
  We found that phagocytic activity of Adh-DC was higher than MACS-DC, whereas the mean fluorescence intensity (MFI) of phagocytic cells was higher in MACS-DC (p<0.05).
  
  
  CONCLUSIONS
  : We concluded that it would be important to consider phagocytosis parameters of generated DCs before making any decision about monocyte isolation methods to have fully functional DCs.
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
authors.forename: Nowruz
authors.initials: N
authors.lastname: Delirezh
authors.forename: Ehsan
authors.initials: E
authors.lastname: Shojaeefar
authors.forename: Parva
authors.initials: P
authors.lastname: Parvin
authors.forename: Behnaz
authors.initials: B
authors.lastname: Asadi
keywords: Adherence; Dendritic Cells; MACS; Monocyte; Phagocytosis
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