title: Proteolytic processing of Bacillus thuringiensis toxin Cry1Ab in rice brown planthopper, Nilaparvata lugens (Stål).
abstract: To understand the low toxicity of Cry toxins in planthoppers, proteolytic activation of Cry1Ab in Nilaparvata lugens was studied. The proteolytic processing of Cry1Ab protoxin by N. lugens midgut proteases was similar to that by trypsin activated Cry1Ab. The Cry1Ab processed with N. lugens midgut proteases was highly insecticidal against Plutella xylostella. However, Cry1Ab activated either by trypsin or the gut proteases of the brown planthopper showed low toxicity in N. lugens. Binding analysis showed that activated Cry1Ab bound to brush border membrane vesicles (BBMV) from N. lugens at a significantly lower level than to BBMV from P. xylostella.
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, PR China. Electronic address: scientistshao@live.cn.
authors.forename: Ensi
authors.initials: E
authors.lastname: Shao
authors.forename: Sijun
authors.initials: S
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.forename: Xiong
authors.initials: X
authors.lastname: Guan
mesh_terms: D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000378:metabolism*; D001665:Binding Sites; D004731:Endotoxins / Q000378:metabolism*; D006430:Hemiptera / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000378:metabolism*; D007300:Insect Control; D019476:Insect Proteins / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D059748:Proteolysis
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D019476:Insect Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D010447:Peptide Hydrolases
keywords: Bacillus thuringiensis; Cry1Ab toxin; Gut proteases; Nilaparvata lugens; Proteolytic processing