title: The role of caveolae in regulating calcitonin receptor-like receptor subcellular distribution in vascular smooth muscle cells.
abstract: To determine whether caveolae and caveolin-1 affect the distribution of calcitonin receptor-like receptors (CLR) in vascular smooth muscle cell (VSMC) membranes, we have used VSMCs cell line A10. We found that calcitonin gene-related peptide (CGRP) reduced CLR protein in the VSMC membrane in a time-dependent manner, which was dramatically decreased after 4 h CGRP treatment, and remained at a low level after 16 h. CGRP8-37 or β-cyclodextrin (β-CD) blocked this effect, without changing the total levels of CLR protein and mRNA in the cells. Co-immunoprecipitation experiments showed that CLR bound to caveolin-1 in cell membrane fractions. Confocal laser microscopic studies confirmed this co-localization relationship at the cell plasma membrane. Thus, our data indicate that the structural integrity of caveolae plays an important role in regulating subcellular distribution of CLR.
authors.affiliation: a Institute of Pharmacy & Pharmacology, Key Laboratory for Arteriosclerology of Hunan Province, University of South China, Hengyang 421001, P. R. China.
authors.forename: Jiang Qiong
authors.initials: JQ
authors.lastname: Tang
authors.forename: Fei
authors.initials: F
authors.lastname: Sun
authors.forename: Yu Hua
authors.initials: YH
authors.lastname: Wang
authors.forename: Lin Xi
authors.initials: LX
authors.lastname: Chen
authors.forename: Chao Hua
authors.initials: CH
authors.lastname: Yao
authors.forename: Xiao Qin
authors.initials: XQ
authors.lastname: Fu
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Xu Ping
authors.initials: XP
authors.lastname: Qin
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism* / Q000494:pharmacology; D058286:Calcitonin Receptor-Like Protein / Q000235:genetics / Q000378:metabolism*; D021941:Caveolae / Q000378:metabolism; D051242:Caveolin 1 / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D051379:Mice; D008881:Migraine Disorders / Q000188:drug therapy; D008916:Miotics / Q000494:pharmacology; D009020:Morphine / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism; D064449:Sequestering Agents / Q000494:pharmacology; D015398:Signal Transduction; D047392:beta-Cyclodextrins / Q000494:pharmacology
chemical_list: D058286:Calcitonin Receptor-Like Protein; D051242:Caveolin 1; D008916:Miotics; D010446:Peptide Fragments; D012333:RNA, Messenger; D064449:Sequestering Agents; D047392:beta-Cyclodextrins; C058636:calcitonin gene-related peptide (8-37); D009020:Morphine; D015740:Calcitonin Gene-Related Peptide; C031215:betadex