title: Reduction of microRNA-206 contributes to the development of bronchopulmonary dysplasia through up-regulation of fibronectin 1.
abstract: OBJECTIVE
  To characterize microRNA-206 (miR-206) in the development of bronchopulmonary dysplasia (BPD).
  
  
  METHODS
  We assessed the expression of miR-206 in BPD mouse lung tissues and blood samples of BPD patients by quantitative real-time PCR. Then, the role of miR-206 in regulating cell biology were examined by XTT assay, flow cytometry, transwell invasion assay, wound healing assay and adhesion assay in vitro. Furthermore, luciferase reporter assay, real-time PCR, western blot and Immunofluorescence staining were performed to figure out the target gene of miR-206.
  
  
  RESULTS
  A reduction in expression of miR-206 was observed in BPD mice compared with controls and in BPD patients compared with controls. miR-206 overexpression significantly induced cell apoptosis, reduced cell proliferation, migration and adhesion abilities, whereas the inhibition of miR-206 expression had the opposite effect. Fibronectin 1 (FN1) is a direct target of miR-206, and fn 1 can be transcriptionally and translationally regulated by miR-206. Down-regulation of miR-206 modulates biological functions of the cells, at least in part, by increasing the level of fn 1. Furthermore, fn 1 expression levels were increased in the BPD mice and BPD patients.
  
  
  CONCLUSIONS
  The expression of miR-206 and its target gene, fn 1, may contribute to the progression of BPD.
authors.affiliation: Department of Pediatrics, BaYi Children's Hospital of The General Military Hospital of Beijing PLA, Beijing, P. R. China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Junjie
authors.initials: J
authors.lastname: Wang
authors.forename: Ludwig
authors.initials: L
authors.lastname: Gortner
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Wei
authors.forename: Jie
authors.initials: J
authors.lastname: Song
authors.forename: Yupei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Li
authors.forename: Zhichun
authors.initials: Z
authors.lastname: Feng
mesh_terms: D000818:Animals; D001997:Bronchopulmonary Dysplasia / Q000235:genetics* / Q000378:metabolism*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D005353:Fibronectins / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism; D014945:Wound Healing
chemical_list: C067625:FN1 protein, human; D005353:Fibronectins; C546087:MIRN206 microRNA, human; D035683:MicroRNAs; C540669:Mirn206 microRNA, mouse; D034741:RNA, Small Interfering
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