title: miR-125b inhibits Connexin43 and promotes glioma growth.
abstract: MicroRNA is strongly associated with tumor growth and development. This study examined the potential roles of miR-125b in glioma growth. We found that miR-125b promotes glioma cell line growth and clone formation, and protects the glioma cells from apoptosis in vitro. The miR-125b-transfected glioma cells also demonstrated increased growth after in vivo transplantation. We further identified that miR-125b inhibits Connexin43 expression, and the overexpression of Connexin43 antagonizes the effects of miR-125b in cell growth and anti-apoptosis. We conclude that miR-125b regulates glioma growth partly through Connexin43 protein.
authors.affiliation: Department of Neurosurgery, The First Hospital, Jilin University, Jilin, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jin
authors.forename: Songbai
authors.initials: S
authors.lastname: Xu
authors.forename: Hongquan
authors.initials: H
authors.lastname: Yu
authors.forename: Boyu
authors.initials: B
authors.lastname: Yang
authors.forename: Hongguang
authors.initials: H
authors.lastname: Zhao
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002999:Clone Cells; D018031:Connexin 43 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D003606:Dacarbazine / Q000031:analogs & derivatives / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005910:Glioma / Q000235:genetics* / Q000473:pathology*; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000187:drug effects / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D000077204:Temozolomide
chemical_list: D020413:3' Untranslated Regions; D018031:Connexin 43; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D003606:Dacarbazine; D000077204:Temozolomide
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