title: Secondary structure of a conserved domain in the intron of influenza A NS1 mRNA.
abstract: Influenza A virus is a segmented single-stranded (-)RNA virus that causes substantial annual morbidity and mortality. The transcriptome of influenza A is predicted to have extensive RNA secondary structure. The smallest genome segment, segment 8, encodes two proteins, NS1 and NEP, via alternative splicing. A conserved RNA domain in the intron of segment 8 may be important for regulating production of NS1. Two different multi-branch loop structures have been proposed for this region. A combination of in vitro chemical mapping and isoenergetic microarray techniques demonstrate that the consensus sequence for this region folds into a hairpin conformation. These results provide an alternative folding for this region and a foundation for designing experiments to probe its functional role in the influenza life cycle.
authors.affiliation: Department of Chemistry and Center for RNA Biology, University of Rochester, Rochester, New York, United States of America.
authors.forename: Salvatore F
authors.initials: SF
authors.lastname: Priore
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Kierzek
authors.forename: Ryszard
authors.initials: R
authors.lastname: Kierzek
authors.forename: Jayson R
authors.initials: JR
authors.lastname: Baman
authors.forename: Walter N
authors.initials: WN
authors.lastname: Moss
authors.forename: Lumbini I
authors.initials: LI
authors.lastname: Dela-Moss
authors.forename: Douglas H
authors.initials: DH
authors.lastname: Turner
mesh_terms: D017398:Alternative Splicing / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics*; D006801:Humans; D009980:Influenza A virus / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral
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