title: Effect of pH on growth and biochemical responses of Dunaliella bardawil and Chlorella ellipsoidea.
abstract: The goal of the present investigation was to study the effect of pH on growth and biochemical responses of Dunaliella bardawil and Chlorella ellipsoidea when exposed to different pH values. The two tested microalgae could grow in a wide range of pH (4-9 for D. bardawil and 4-10 for C. ellipsoidea). The dry weight gain and the biochemical components of D. bardawil were greatly enhanced at pH 7.5. In contrast, dry weight and carbohydrate content of C. ellipsoidea attained their maximum values at the alkaline pH. On the other hand, the protein content of C. ellipsoidea recorded its highest value at pH 4, while the pigment content of the same alga was highest at pH 4, 6, and 7.5 and decreased at alkaline pH. Both pH 6 and pH 9 stimulated the accumulation of β-carotene, vitamin E and vitamin C in D. bardawil, with the highest values of the three compounds recorded at pH 9. In the case of C. ellipsoidea, β-carotene content increased at pH 6 and pH 10 as compared with the control, but the amount of β-carotene was much higher at pH 6 than at pH 10. Vitamin E content was higher in C. ellipsoidea cells at pH 10 than at pH 6. Both pH 6 and pH 10 caused a significant decline in vitamin C content of C. ellipsoidea.
authors.affiliation: Department of Botany, Faculty of Science, Cairo University, Cairo, Egypt.
authors.forename: Zeinab I
authors.initials: ZI
authors.lastname: Khalil
authors.forename: Mohsen M S
authors.initials: MM
authors.lastname: Asker
authors.forename: Salwa
authors.initials: S
authors.lastname: El-Sayed
authors.forename: Imam A
authors.initials: IA
authors.lastname: Kobbia
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