title: Folding of 16S rRNA in a signal-producing structure for the detection of bacteria.
abstract: Sixty-four DNA strands hybridize to 16S rRNA to form 32 deoxyribozyme catalytic cores that produce a fluorescent signal. The approach allows detection of 0.6 pM 16S rRNA, or about 3×10(4) bacterial cells in a PCR-free format.
authors.affiliation: Chemistry Department, University of Central Florida, 4000 Central Florida Blvd, Orlando, FL 32816 (USA). yugeras@gmail.com.
authors.forename: Yulia V
authors.initials: YV
authors.lastname: Gerasimova
authors.forename: Dmitry M
authors.initials: DM
authors.lastname: Kolpashchikov
mesh_terms: D001419:Bacteria / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D021881:DNA, Catalytic / Q000737:chemistry; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000737:chemistry* / Q000235:genetics
chemical_list: D021881:DNA, Catalytic; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA; DNA origami; binary deoxyribozyme; detection of bacteria; fluorescent sensors
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