title: Bioconversion of phosphatidylserine by phospholipase D from Streptomyces racemochromogenes in a microaqueous water-immiscible organic solvent.
abstract: Phospholipase D (PLD)-mediated transphosphatidylation of phosphatidylcholine (PC) in a biphasic system was limited by the hydrolysis reaction. A biphasic system can produce a large amount of water. To solve this problem, a microaqueous water-immiscible organic solvent was used for the first time in the bioconversion of phosphatidylserine (PS). The transphosphatidylation among 40 µmol PC, 800 µmol L-serine, and 0.17 U/mL PLD in 2.133 mL of butyl acetate with 6.25% water (V/V) was conducted at a trans-phosphatidylation rate of 88% (mol/mol), and no hydrolytic reaction was observed. Compared to commonly used biphasic systems, this system shows a similar transphosphatidylation rate, whereas the undesirable hydrolysis of phospholipids was completely suppressed.
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology.
authors.forename: Sheng
authors.initials: S
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Ning
authors.initials: N
authors.lastname: Hao
authors.forename: Ming
authors.initials: M
authors.lastname: Yan
mesh_terms: D006868:Hydrolysis; D009930:Organic Chemicals / Q000737:chemistry*; D010718:Phosphatidylserines / Q000378:metabolism*; D010739:Phospholipase D / Q000378:metabolism*; D012997:Solvents / Q000737:chemistry*; D013302:Streptomyces / Q000201:enzymology*; D014867:Water / Q000737:chemistry*
chemical_list: D009930:Organic Chemicals; D010718:Phosphatidylserines; D012997:Solvents; D014867:Water; D010739:Phospholipase D