title: Homologue gene of bile acid transporters ntcp, asbt, and ost-alpha in rainbow trout Oncorhynchus mykiss: tissue expression, effect of fasting, and response to bile acid administration.
abstract: Bile acid transporters belonging to the SLC10A protein family, Na+ taurocholate cotransporting polypeptide (NTCP or SLC10A1), apical sodium-dependent bile salt transporter (ASBT or SLC10A2), and organic solute transporter alpha (Ost-alpha) have been known to play critical roles in the enterohepatic circulation of bile acids in mammals. In this study, ntcp, asbt, and ost-alpha-1/-2 cDNA were cloned, their tissue distributions were characterized, and the effects of fasting and bile acid administration on their expression were examined in rainbow trout Oncorhynchus mykiss. The structural characteristics of Ntcp, Asbt, and Ost-alpha were well conserved in trout, and three-dimensional structure analysis showed that Ntcp and Asbt were similar to each other. Tissue distribution analysis revealed that trout asbt was primarily expressed in the hindgut, while ntcp expression occurred in the brain, and ost-alpha-1/-2 was mainly expressed in the liver or ovary. Although asbt and ost-alpha-1 mRNA levels in the gut increased in response to fasting for 4 days, ost-alpha-1 expression in the liver decreased. Similarly, bile acid administration increased asbt and ost-alpha-1 expression levels in the gut, while those of ntcp and ost-alpha-2 in the liver decreased. These results suggested that the genes asbt, ntcp, and ost-alpha are involved in bile acid transport in rainbow trout.
authors.affiliation: Aquaculture Systems Division, National Research Institute of Aquaculture, Fisheries Research Agency, Tamaki, Mie, 519-0423, Japan, kojim@affrc.go.jp.
authors.forename: Koji
authors.initials: K
authors.lastname: Murashita
authors.forename: Yasutoshi
authors.initials: Y
authors.lastname: Yoshiura
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Chisada
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Furuita
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Sugita
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Matsunari
authors.forename: Yasuro
authors.initials: Y
authors.lastname: Iwashita
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001647:Bile Acids and Salts / Q000008:administration & dosage* / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005215:Fasting / Q000378:metabolism; D005260:Female; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D008297:Male; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000378:metabolism*; D029363:Organic Anion Transporters, Sodium-Dependent / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D040681:Structural Homology, Protein; D027981:Symporters / Q000235:genetics* / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D001647:Bile Acids and Salts; D002352:Carrier Proteins; D018076:DNA, Complementary; D029941:Fish Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D029363:Organic Anion Transporters, Sodium-Dependent; D012333:RNA, Messenger; D027981:Symporters; C037643:bile acid binding proteins; C071908:sodium-bile acid cotransporter
references.citation: J Biol Chem. 1998 Dec 25;273(52):34691-5
references.pmid: 9856990
references.citation: J Clin Invest. 1994 Mar;93(3):1326-31
references.pmid: 8132774
references.citation: Hepatology. 2005 Dec;42(6):1270-9
references.pmid: 16317684
references.citation: J Biol Chem. 2003 Jul 25;278(30):27473-82
references.pmid: 12719432
references.citation: J Biol Chem. 2006 Jun 16;281(24):16410-8
references.pmid: 16608845
references.citation: Ann N Y Acad Sci. 1986;464:126-37
references.pmid: 3524346
references.citation: Mol Biol Evol. 2007 Aug;24(8):1596-9
references.pmid: 17488738
references.citation: J Biol Chem. 1995 Nov 10;270(45):27228-34
references.pmid: 7592981
references.citation: Gen Comp Endocrinol. 2011 Feb 1;170(3):494-500
references.pmid: 21078323
references.citation: Biochemistry. 2006 Jan 24;45(3):943-53
references.pmid: 16411770
references.citation: Annu Rev Biochem. 2003;72:137-74
references.pmid: 12543708
references.citation: Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):3891-6
references.pmid: 18292224
references.citation: Am J Physiol Gastrointest Liver Physiol. 2006 Mar;290(3):G476-85
references.pmid: 16269519
references.citation: Am J Physiol Gastrointest Liver Physiol. 2006 May;290(5):G912-22
references.pmid: 16357058
references.citation: J Lipid Res. 2012 Aug;53(8):1535-42
references.pmid: 22669917
references.citation: Gut. 2003 Sep;52(9):1239-41
references.pmid: 12912852
references.citation: Am J Physiol. 1994 Sep;267(3 Pt 1):G380-6
references.pmid: 7943234
references.citation: Annu Rev Physiol. 2002;64:635-61
references.pmid: 11826283
references.citation: Semin Liver Dis. 2000;20(3):273-92
references.pmid: 11076396
references.citation: Exp Biol Med (Maywood). 2005 Nov;230(10):689-98
references.pmid: 16246895
references.citation: Genome Res. 2003 Mar;13(3):382-90
references.pmid: 12618368
references.citation: Drug Metab Pharmacokinet. 2005 Dec;20(6):452-77
references.pmid: 16415531
references.citation: Am J Physiol. 1998 Jan;274(1):G157-69
references.pmid: 9458785
references.citation: Am J Physiol Gastrointest Liver Physiol. 2008 Jul;295(1):G179-G186
references.pmid: 18497332
references.citation: J Lipid Res. 2006 Jan;47(1):201-14
references.pmid: 16251721
references.citation: Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9431-6
references.pmid: 11470901
references.citation: Biochemistry. 1999 Aug 31;38(35):11379-88
references.pmid: 10471288
references.citation: J Biol Chem. 2005 Feb 25;280(8):6960-8
references.pmid: 15563450
references.citation: Pflugers Arch. 2004 Feb;447(5):566-70
references.pmid: 12851823
references.citation: J Clin Endocrinol Metab. 1996 Apr;81(4):1460-4
references.pmid: 8636351
references.citation: Am J Physiol Gastrointest Liver Physiol. 2006 Jun;290(6):G1124-30
references.pmid: 16423920
references.citation: Biochemistry. 2002 Jun 11;41(23):7253-66
references.pmid: 12044156
references.citation: Am J Physiol. 1998 Feb;274(2):G370-5
references.pmid: 9486191
references.citation: Gen Comp Endocrinol. 2009 Apr;161(2):229-37
references.pmid: 19523397
references.citation: Physiol Rev. 2003 Apr;83(2):633-71
references.pmid: 12663868
references.citation: Hepatology. 2004 Jul;40(1):149-56
references.pmid: 15239098
references.citation: Eur J Biochem. 2003 Mar;270(6):1117-27
references.pmid: 12631271
references.citation: Comp Biochem Physiol C Toxicol Pharmacol. 2010 Mar;151(2):209-15
references.pmid: 19883795
references.citation: Nature. 2011 Oct 05;478(7369):408-11
references.pmid: 21976025
references.citation: J Clin Invest. 1997 Dec 1;100(11):2714-21
references.pmid: 9389734
references.citation: Hepatology. 2009 May;49(5):1403-18
references.pmid: 19296471
references.citation: J Biol Chem. 2004 Jan 23;279(4):2790-9
references.pmid: 14570929
references.citation: Front Biosci (Landmark Ed). 2009 Jan 01;14:2829-44
references.pmid: 19273238