title: Induction and analysis of epithelial to mesenchymal transition.
abstract: Epithelial to mesenchymal transition (EMT) is essential for proper morphogenesis during development. Misregulation of this process has been implicated as a key event in fibrosis and the progression of carcinomas to a metastatic state. Understanding the processes that underlie EMT is imperative for the early diagnosis and clinical control of these disease states. Reliable induction of EMT in vitro is a useful tool for drug discovery as well as to identify common gene expression signatures for diagnostic purposes. Here we demonstrate a straightforward method for the induction of EMT in a variety of cell types. Methods for the analysis of cells pre- and post-EMT induction by immunocytochemistry are also included. Additionally, we demonstrate the effectiveness of this method through antibody-based array analysis and migration/invasion assays.
authors.affiliation: Antibody Development Department, R&D Systems, Inc.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Tang
authors.forename: Greg
authors.initials: G
authors.lastname: Herr
authors.forename: Wade
authors.initials: W
authors.lastname: Johnson
authors.forename: Ernesto
authors.initials: E
authors.lastname: Resnik
authors.forename: Joy
authors.initials: J
authors.lastname: Aho
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*
references.citation: J Biomed Biotechnol. 2011;2011:567305
references.pmid: 22007144
references.citation: J Clin Invest. 2009 Jun;119(6):1420-8
references.pmid: 19487818
references.citation: Cancer Res. 2008 May 15;68(10):3645-54
references.pmid: 18483246
references.citation: Cancer Res. 2010 Oct 1;70(19):7360-4
references.pmid: 20823151
references.citation: Cancer Res. 2012 Mar 15;72(6):1449-58
references.pmid: 22271686
references.citation: Respir Res. 2005 Jun 09;6:56
references.pmid: 15946381
references.citation: J Cell Biol. 1994 Dec;127(6 Pt 2):2021-36
references.pmid: 7806579
references.citation: J Cell Biol. 2005 Jan 3;168(1):29-33
references.pmid: 15631989
references.citation: Cell Res. 2009 Feb;19(2):156-72
references.pmid: 19153598
references.citation: Nat Rev Cancer. 2002 Jun;2(6):442-54
references.pmid: 12189386
references.citation: Cancer Res. 2008 Aug 15;68(16):6524-32
references.pmid: 18701475
references.citation: Breast Cancer Res. 2004;6(3):R215-31
references.pmid: 15084245
references.citation: Proc Natl Acad Sci U S A. 2010 Aug 31;107(35):15449-54
references.pmid: 20713713
references.citation: Nat Rev Cancer. 2009 Apr;9(4):265-73
references.pmid: 19262571
references.citation: Neoplasia. 2004 Sep-Oct;6(5):603-10
references.pmid: 15548370
references.citation: Cell. 2011 Jun 10;145(6):926-40
references.pmid: 21663795
references.citation: Dev Biol. 1990 Oct;141(2):456-60
references.pmid: 1698672
references.citation: Cold Spring Harb Perspect Biol. 2010 Feb;2(2):a002915
references.pmid: 20182623