title: Brucella canis infection in dogs attended in veterinary clinics from patos, ParabaĪBA state, Brazil.
abstract: To determine the frequency of anti-Brucella canis antibodies in dogs attended in veterinary clinics from Patos, Paraíba State, Brazil, as well as to identify risk factors and to isolate and identify the agent, 193 dogs were used. Agar gel immunodiffusion test (AGID) was used to detect B. canis antibodies in sera. Isolation of B. canis was carried out in blood and bone marrow from seropositive animals. Six animals tested seropositive in AGID, resulting in a frequency of 3.11%. B. canis was isolated from bone marrow of one seropositive animal, with confirmation by PCR. Lack of cleaning of the dog's environment was identified as risk factor (odds ratio = 7.91). This is the first report of isolation of B. canis in dogs from the Northeast region of Brazil.
authors.affiliation: Laboratório de Doenças Transmissíveis, Unidade Acadêmica de Medicina Veterinária, Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande , Patos, PB , Brasil.
authors.forename: Annielle Regina Fonseca
authors.initials: AR
authors.lastname: Fernandes
authors.forename: Sérgio Santos
authors.initials: SS
authors.lastname: de Azevedo
authors.forename: Eliana Scarcelli
authors.initials: ES
authors.lastname: Pinheiro
authors.forename: Margareth Élide
authors.initials: MÉ
authors.lastname: Genovez
authors.forename: Adílio Santos
authors.initials: AS
authors.lastname: de Azevedo
authors.forename: Carolina
authors.initials: C
authors.lastname: de Sousa Américo Batista
authors.forename: Clebert José
authors.initials: CJ
authors.lastname: Alves
keywords: Bacterial diseases; canine brucellosis; microbiological culture; prevalence; risk factors
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