title: Diversity of methicillin-resistant Staphylococcus aureus strains isolated from residents of 26 nursing homes in Orange County, California.
abstract: Nursing homes represent a unique and important methicillin-resistant Staphylococcus aureus (MRSA) reservoir. Not only are strains imported from hospitals and the community, strains can be transported back into these settings from nursing homes. Since MRSA bacteria are prevalent in nursing homes and yet relatively poorly studied in this setting, a multicenter, regional assessment of the frequency and diversity of MRSA in the nursing home reservoir was carried out and compared to that of the MRSA from hospitals in the same region. The prospective study collected MRSA from nasal swabbing of residents of 26 nursing homes in Orange County, California, and characterized each isolate by spa typing. A total of 837 MRSA isolates were collected from the nursing homes. Estimates of admission prevalence and point prevalence of MRSA were 16% and 26%, respectively. The spa type genetic diversity was heterogeneous between nursing homes and significantly higher overall (77%) than the diversity in Orange County hospitals (72%). MRSA burden in nursing homes appears largely due to importation from hospitals. As seen in Orange County hospitals, USA300 (sequence type 8 [ST8]/t008), USA100 (ST5/t002), and a USA100 variant (ST5/t242) were the dominant MRSA clones in Orange County nursing homes, representing 83% of all isolates, although the USA100 variant was predominant in nursing homes, whereas USA300 was predominant in hospitals. Control strategies tailored to the complex problem of MRSA transmission and infection in nursing homes are needed in order to minimize the impact of this unique reservoir on the overall regional MRSA burden.
authors.affiliation: Department of Infectious Disease Epidemiology, Imperial College London, London, United Kingdom.
authors.forename: Lyndsey O
authors.initials: LO
authors.lastname: Hudson
authors.forename: Courtney
authors.initials: C
authors.lastname: Reynolds
authors.forename: Brian G
authors.initials: BG
authors.lastname: Spratt
authors.forename: Mark C
authors.initials: MC
authors.lastname: Enright
authors.forename: Victor
authors.initials: V
authors.lastname: Quan
authors.forename: Diane
authors.initials: D
authors.lastname: Kim
authors.forename: Paul
authors.initials: P
authors.lastname: Hannah
authors.forename: Lydia
authors.initials: L
authors.lastname: Mikhail
authors.forename: Richard
authors.initials: R
authors.lastname: Alexander
authors.forename: Douglas F
authors.initials: DF
authors.lastname: Moore
authors.forename: Daniel
authors.initials: D
authors.lastname: Godoy
authors.forename: Cynthia J
authors.initials: CJ
authors.lastname: Bishop
authors.forename: Susan S
authors.initials: SS
authors.lastname: Huang
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002140:California / Q000453:epidemiology; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006761:Hospitals; D006801:Humans; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008875:Middle Aged; D017720:Molecular Epidemiology; D058889:Molecular Typing; D009296:Nasal Cavity / Q000382:microbiology; D009735:Nursing Homes; D015995:Prevalence; D011446:Prospective Studies; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology*; D013205:Staphylococcal Protein A / Q000235:genetics
chemical_list: D013205:Staphylococcal Protein A
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