title: Eunicellin-based diterpenoids from the Formosan soft coral Klyxum molle with inhibitory activity on superoxide generation and elastase release by neutrophils.
abstract: Eleven new eunicellin-based diterpenoids possessing a cladiellane skeleton with a C-2, C-9 ether bridge, klymollins I-S (1-11), have been isolated from the EtOAc extract of the soft coral Klyxum molle from Taiwan waters. The structures of compounds 1-11 were elucidated by extensive spectroscopic analysis, including 2D NMR spectroscopy (COSY, HSQC, HMBC, and NOESY). Compound 5 exhibited cytotoxicity toward several cancer cell lines. Compound 5 is the first eunicellin-based metabolite bearing a phenyl group and displays significant inhibition of both superoxide anion generation and elastase release in N-formyl-methionyl-leucyl-phenylalanine/cytochalasin B (fMLP/CB)-induced human neutrophils.
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University , Kaohsiung 804, Taiwan , Republic of China.
authors.forename: Ming-Chang
authors.initials: MC
authors.lastname: Lin
authors.forename: Bo-Wei
authors.initials: BW
authors.lastname: Chen
authors.forename: Chiung-Yao
authors.initials: CY
authors.lastname: Huang
authors.forename: Chang-Feng
authors.initials: CF
authors.lastname: Dai
authors.forename: Tsong-Long
authors.initials: TL
authors.lastname: Hwang
authors.forename: Jyh-Horng
authors.initials: JH
authors.lastname: Sheu
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D003571:Cytochalasin B / Q000494:pharmacology; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D015394:Molecular Structure; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D010196:Pancreatic Elastase / Q000032:analysis / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013624:Taiwan
chemical_list: D000970:Antineoplastic Agents; D004224:Diterpenes; C476503:eunicellin; C584988:klymollin M; D013481:Superoxides; D003571:Cytochalasin B; D009240:N-Formylmethionine Leucyl-Phenylalanine; D010196:Pancreatic Elastase