title: Nanotechnology in plant disease management: DNA-directed silver nanoparticles on graphene oxide as an antibacterial against Xanthomonas perforans.
abstract: Bacterial spot caused by Xanthomonas perforans is a major disease of tomatoes, leading to reduction in production by 10-50%. While copper (Cu)-based bactericides have been used for disease management, most of the X. perforans strains isolated from tomatoes in Florida and other locations worldwide are Cu-resistant. We have developed DNA-directed silver (Ag) nanoparticles (NPs) grown on graphene oxide (GO). These Ag@dsDNA@GO composites effectively decrease X. perforans cell viability in culture and on plants. At the very low concentration of 16 ppm of Ag@dsDNA@GO, composites show excellent antibacterial capability in culture with significant advantages in improved stability, enhanced antibacterial activity, and stronger adsorption properties. Application of Ag@dsDNA@GO at 100 ppm on tomato transplants in a greenhouse experiment significantly reduced the severity of bacterial spot disease compared to untreated plants, giving results similar to those of the current grower standard treatment, with no phytotoxicity.
authors.affiliation: Center for Research at the Bio/Nano Interface, Department of Chemistry and Shands Cancer Center, University of Florida , Gainesville, Florida 32611, United States, and Plant Pathology Lab, North Florida Research and Education Center, University of Florida , Quincy, Florida 32351, United States.
authors.forename: Ismail
authors.initials: I
authors.lastname: Ocsoy
authors.forename: Mathews L
authors.initials: ML
authors.lastname: Paret
authors.forename: Muserref Arslan
authors.initials: MA
authors.lastname: Ocsoy
authors.forename: Sanju
authors.initials: S
authors.lastname: Kunwar
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Mingxu
authors.initials: M
authors.lastname: You
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D006108:Graphite / Q000737:chemistry / Q000494:pharmacology*; D053768:Metal Nanoparticles; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology; D010087:Oxides / Q000737:chemistry*; D010935:Plant Diseases / Q000628:therapy*; D012834:Silver / Q000737:chemistry*; D014974:Xanthomonas / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D010087:Oxides; D012834:Silver; D006108:Graphite
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