title: [Rapid enrichment and cultivation of denitrifying phosphate-removal bacteria and its identification by fluorescence in situ hybridization technology].
abstract: The present work focused on a rapid enrichment and cultivation of denitrifying phosphate-removal bacteria (DPB) in a membrane bio-reactor(MBR) by using A2/O anaerobic sludge from a wastewater treatment plant as seed, as well as providing an identification method. In the experiments, sodium acetate was used as the carbon source and a certain amount of nitrate was added to the MBR in the anoxic stage. Results showed that, with the efficient trap of the hollow-fiber membrane module, the proportion of DPB in all the phosphate-accumulating organisms (PAOs) increased from 24% to 93% within 35 days after two-stage's cultivation including anaerobic/aerobic and anaerobic/anoxic, during which the removal efficiency of nitrogen and phosphorus reached more than 90%. The activated sludge was identified by combining a regular method and the fluorescence in situ hybridization (FISH) technique, which demonstrated that Pseudomonas sp. and Rhodocyclus sp. were the dominant bacteria in the used bioreactor.
authors.affiliation: School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, China. liuli19880128@163.com
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Bing
authors.initials: B
authors.lastname: Tang
authors.forename: Shao-Song
authors.initials: SS
authors.lastname: Huang
authors.forename: Feng-Lian
authors.initials: FL
authors.lastname: Fu
authors.forename: Qi-Qin
authors.initials: QQ
authors.lastname: Zhang
authors.forename: Jian-Bin
authors.initials: JB
authors.lastname: Li
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Luo
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D058440:Denitrification; D017404:In Situ Hybridization, Fluorescence; D009584:Nitrogen / Q000302:isolation & purification* / Q000378:metabolism; D010758:Phosphorus / Q000302:isolation & purification* / Q000378:metabolism; D011549:Pseudomonas / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D043362:Rhodocyclaceae / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D012722:Sewage / Q000382:microbiology*; D064091:Waste Disposal Facilities; D014865:Waste Disposal, Fluid / Q000379:methods
chemical_list: D012722:Sewage; D010758:Phosphorus; D009584:Nitrogen