title: Antigen-specific in vitro immunization: a source for human monoclonal antibodies.
abstract: Human monoclonal antibody has great potential for treatment of various diseases utilizing their specificity against antigens. We have shown an in vitro immunization (IVI) protocol inducing antigen-specific immune responses in human peripheral blood mononuclear cells (PBMCs) for efficient production of human monoclonal antibodies. By using IVI method antigen specific antibody genes can be efficiently obtained because of increasing production of antigen-specific antibodies from in vitro immunized PBMCs. This IVI protocol will be widely applied for combination with several display methods and enhance the production of human monoclonal antibodies.
authors.affiliation: Faculty of Agriculture, Department of Bioscience and Biotechnology, Kyushu University, Fukuoka, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Tomimatsu
authors.forename: Sanetaka
authors.initials: S
authors.lastname: Shirahata
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D000918:Antibody Specificity / Q000235:genetics / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D006801:Humans; D007114:Immunization / Q000379:methods; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens