title: Temperature and carbon assimilation regulate the chlorosome biogenesis in green sulfur bacteria.
abstract: Green photosynthetic bacteria adjust the structure and functionality of the chlorosome-the light-absorbing antenna complex-in response to environmental stress factors. The chlorosome is a natural self-assembled aggregate of bacteriochlorophyll (BChl) molecules. In this study, we report the regulation of the biogenesis of the Chlorobaculum tepidum chlorosome by carbon assimilation in conjunction with temperature changes. Our studies indicate that the carbon source and thermal stress culture of C. tepidum grows slower and incorporates fewer BChl c in the chlorosome. Compared with the chlorosome from other cultural conditions we investigated, the chlorosome from the carbon source and thermal stress culture displays (a) smaller cross-sectional radius and overall size, (b) simplified BChl c homologs with smaller side chains, (c) blue-shifted Qy absorption maxima, and (d) a sigmoid-shaped circular dichroism spectra. Using a theoretical model, we analyze how the observed spectral modifications can be associated with structural changes of BChl aggregates inside the chlorosome. Our report suggests a mechanism of metabolic regulation for chlorosome biogenesis.
authors.affiliation: School of Chemistry and Biochemistry, Clark University, Worcester, Massachusetts. Electronic address: jtang@clarku.edu.
authors.forename: Joseph Kuo-Hsiang
authors.initials: JK
authors.lastname: Tang
authors.forename: Semion K
authors.initials: SK
authors.lastname: Saikin
authors.forename: Sai Venkatesh
authors.initials: SV
authors.lastname: Pingali
authors.forename: Miriam M
authors.initials: MM
authors.lastname: Enriquez
authors.forename: Joonsuk
authors.initials: J
authors.lastname: Huh
authors.forename: Harry A
authors.initials: HA
authors.lastname: Frank
authors.forename: Volker S
authors.initials: VS
authors.lastname: Urban
authors.forename: Alán
authors.initials: A
authors.lastname: Aspuru-Guzik
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D001429:Bacteriochlorophylls / Q000378:metabolism; D002244:Carbon / Q000378:metabolism*; D019414:Chlorobi / Q000378:metabolism*; D008958:Models, Molecular; D055620:Optical Phenomena; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D002244:Carbon
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