title: Early postweaning exercise improves central leptin sensitivity in offspring of rat dams fed high-fat diet during pregnancy and lactation.
abstract: Maternal high-fat (HF) diet has long-term consequences on the metabolic phenotype of the offspring. Here, we determined the effects of postweaning exercise in offspring of rat dams fed HF diet during gestation and lactation. Pregnant Sprague-Dawley rats were maintained on chow or HF diet throughout gestation and lactation. All pups were weaned onto chow diet on postnatal day (PND) 21. At 4 wk of age, male pups were given free access to running wheels (RW) or remained sedentary (SED) for 3 wk, after which all rats remained sedentary, resulting in four groups: CHOW-SED, CHOW-RW, HF-SED, and HF-RW. Male HF offspring gained more body weight by PND7 compared with CHOW pups and maintained this weight difference through the entire experiment. Three weeks of postweaning exercise did not affect body weight gain in either CHOW or HF offspring, but reduced adiposity in HF offspring. Plasma leptin was decreased at the end of the 3-wk running period in HF-RW rats but was not different from HF-SED 9 wk after the exercise period ended. At 14 wk of age, intracerebroventricular injection of leptin suppressed food intake in CHOW-SED, CHOW-RW, and HF-RW, while it did not affect food intake in HF-SED group. At death, HF-RW rats also had higher leptin-induced phospho-STAT3 level in the arcuate nucleus than HF-SED rats. Both maternal HF diet and postweaning exercise had effects on hypothalamic neuropeptide and receptor mRNA expression in adult offspring. Our data suggest that postweaning exercise improves central leptin sensitivity and signaling in this model.
authors.affiliation: Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi, People's Republic of China; and.
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.forename: Nu-Chu
authors.initials: NC
authors.lastname: Liang
authors.forename: Erin R
authors.initials: ER
authors.lastname: Ewald
authors.forename: Ryan H
authors.initials: RH
authors.lastname: Purcell
authors.forename: Gretha J
authors.initials: GJ
authors.lastname: Boersma
authors.forename: Jianqun
authors.initials: J
authors.lastname: Yan
authors.forename: Timothy H
authors.initials: TH
authors.lastname: Moran
authors.forename: Kellie L K
authors.initials: KL
authors.lastname: Tamashiro
mesh_terms: D050154:Adiposity; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism* / Q000503:physiopathology; D059305:Diet, High-Fat / Q000009:adverse effects*; D004435:Eating; D005260:Female; D005786:Gene Expression Regulation; D005951:Glucose Tolerance Test; D007276:Injections, Intraventricular; D007328:Insulin / Q000097:blood; D007774:Lactation / Q000378:metabolism*; D020738:Leptin / Q000008:administration & dosage / Q000097:blood*; D008297:Male; D039382:Maternal Nutritional Physiological Phenomena; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism; D009765:Obesity / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D010766:Phosphorylation; D005082:Physical Exertion; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018013:Receptors, Neuropeptide / Q000235:genetics / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D057185:Sedentary Behavior; D015398:Signal Transduction; D014886:Weaning; D015430:Weight Gain
chemical_list: D001786:Blood Glucose; D007328:Insulin; D020738:Leptin; D009479:Neuropeptides; D012333:RNA, Messenger; D018013:Receptors, Neuropeptide; D050796:STAT3 Transcription Factor; C494088:Stat3 protein, rat
keywords: development; exercise; leptin; maternal diet; obesity
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