title: Aeromonas presence in drinking water from collective reservoirs and wells in peri-urban area in Brazil.
abstract: Aeromonas genus is considered an emerging pathogen and its presence in drinking water supplies is a reason to public health concern. This study investigated the occurrence of Aeromonas in samples from collective reservoirs and wells used as drinking water sources in a peri-urban area. A total of 35 water samples were collected from collective reservoirs and 32 from wells bimonthly, from September 2007 to September 2008. Aeromonas spp determination was carried out using a Multiple-Tube Technique. Samples were inoculated into alkaline peptone water and the superficial film formed was transferred to blood agar plates amended with ampicillin. Typical Aeromonas colonies were submitted to a biochemical screening and then to biochemical tests for species differentiation. Aeromonas was detected in 13 (19%) of the 69 samples examined (6 from collective reservoirs and 7 from wells). Concentrations of Aeromonas in collective reservoirs ranged from <0.3 to 1.2 x10(2)MPN/100mL and, in wells, from <0.3 to 2.4 x10(2)MPN/100mL. The most frequent specie in the collective reservoir samples was Aeromonas spp (68%), followed by A. encheleia (14%) and A. allosaccharophila (8%) and A. hydrophila (8%). Aeromonas spp (87%) was the most frequent specie isolated from well samples, followed by A. allosacchariphila (8%), A. encheleia (2%) and A. jandaei (5%). These data show the presence and diversity of Aeromonas genus in the samples analyzed and highlight that its presence in drinking water poses a significant public health concern.
authors.affiliation: Faculdade de Saúde Pública, Universidade de São Paulo , São Paulo, SP , Brasil .
authors.forename: Maria Tereza
authors.initials: MT
authors.lastname: Pepe Razzolini
authors.forename: Wanda Maria
authors.initials: WM
authors.lastname: Risso Günther
authors.forename: Solange
authors.initials: S
authors.lastname: Martone-Rocha
authors.forename: Heloísa
authors.initials: H
authors.lastname: Duarte de Luca
authors.forename: Maria Regina
authors.initials: MR
authors.lastname: Alves Cardoso
keywords: Aeromonas; peri-urban area; water quality
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