title: Multi-petal cyclamen flowers produced by AGAMOUS chimeric repressor expression.
abstract: Cyclamen persicum (cyclamen) is a commercially valuable, winter-blooming perennial plant. We cloned two cyclamen orthologues of AGAMOUS (AG), CpAG1 and CpAG2, which are mainly expressed in the stamen and carpel, respectively. Cyclamen flowers have 5 petals, but expression of a chimeric repressor of CpAG1 (CpAG1-SRDX) caused stamens to convert into petals, resulting in a flower with 10 petals. By contrast, CpAG2-SRDX only caused incomplete formation of stamens and carpels. Expression in Arabidopsis thaliana showed similar effects on flower organ specification. Simultaneous expression of CpAG1-SRDX and CpAG2-SRDX in cyclamen induced rose-like, multi-petal flowers, a potentially valuable trait in commercial ornamental varieties. Expression of CpAG2-SRDX in a cyclamen mutant lacking expression of CpAG1 more effectively produced multi-petal flowers. Here, we controlled the number of petals in cyclamen by simple genetic engineering with a chimeric repressor. This strategy may be applicable useful for other ornamental plants with two distinct AG orthologues.
authors.affiliation: Central Research Laboratories, Hokko Chemical Industry Co., Ltd, 2165 Toda, Atsugi, Kanagawa 243-0023, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Oshima
authors.forename: Tomomichi
authors.initials: T
authors.lastname: Yamamura
authors.forename: Masao
authors.initials: M
authors.lastname: Sugiyama
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Mitsuda
authors.forename: Norihiro
authors.initials: N
authors.lastname: Ohtsubo
authors.forename: Masaru
authors.initials: M
authors.lastname: Ohme-Takagi
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Terakawa
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D031824:Cyclamen / Q000235:genetics*; D035264:Flowers / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D026161:MADS Domain Proteins / Q000235:genetics*; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*; D059467:Transcriptome
chemical_list: D026161:MADS Domain Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins
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