title: Isolation and identification of feline calicivirus and feline herpesvirus in Southern Brazil.
abstract: Feline calicivirus (FCV) and feline herpesvirus type 1 (FHV-1) are the two primary causes of upper respiratory tract disease in cats. The aim of this study was to demonstrate the distribution of FCV and FHV-1 among the feline population of several counties in Rio Grande do Sul State, Brazil. To this end, conjunctival and nasal swabs were collected from 302 cats from different locations, including households, breeding catteries, veterinary clinics, animal hospitals and experimental research facilities. The samples were collected between July 2006 to June 2009. The virus isolation was performed in CRFK cells and, subsequently, the identification was confirmed by PCR. FCV, FHV-1, or both were isolated from 55 cats from 28 different locations. FCV alone was isolated from 52.7% (29/55) of the animals that tested positively, FHV-1 alone was isolated from 38.2% (21/55) of the animals that tested positively, and co-infection were detected in 9.1% (5/55) of the animals that tested positively. Virus detection was more prevalent in cats that were less than 1 year old, among animals that shared a living space with other cats, and females. FCV and FHV-1 were isolated from vaccinated cats. In addition, both viruses were isolated from cats that showed no signs of disease. The results suggest that a carrier state is common for both viruses in the evaluated population. A search for other causes of respiratory disease in that population is necessary; and further studies relating to the molecular characterization of viruses and vaccine efficacy are also necessary.
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria , Santa Maria, RS , Brasil ; Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria , Santa Maria, RS , Brasil.
authors.forename: Andréia
authors.initials: A
authors.lastname: Henzel
authors.forename: Mário Celso Sperotto
authors.initials: MC
authors.lastname: Brum
authors.forename: Cláudia
authors.initials: C
authors.lastname: Lautert
authors.forename: Mathias
authors.initials: M
authors.lastname: Martins
authors.forename: Luciane Teresinha
authors.initials: LT
authors.lastname: Lovato
authors.forename: Rudi
authors.initials: R
authors.lastname: Weiblen
keywords: FCV; FHV-1; URTD; epidemiology
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