title: Acid-base and electrolyte status during normovolemic hemodilution with succinylated gelatin or HES-containing volume replacement solutions in rats.
abstract: BACKGROUND
  In the past, several studies have compared different colloidal replacement solutions, whereby the focus was usually on the respective colloid. We therefore systematically studied the influence of the carrier solution's composition of five approved colloidal volume replacement solutions (Gelafundin, Gelafusal, Geloplasma, Voluven and Volulyte) on acid-base as well as electrolyte status during and following acute severe normovolemic hemodilution. The solutions differed in the colloid used (succinylated gelatin vs. HES) and in the presence and concentration of metabolizable anions as well as in their electrolyte composition.
  
  
  METHODS
  Anesthetized Wistar rats were subjected to a stepwise normovolemic hemodilution with one of the solutions until a final hematocrit of 10%. Subsequent to dilution (162 min), animals were observed for an additional period (150 min). During dilution and observation time blood gas analyses were performed eight times in total. Additionally, in the Voluven and Volulyte groups as well as in 6 Gelafundin animals, electrolyte concentrations, glucose, pH and succinylated gelatin were measured in urine and histopathological evaluation of the kidney was performed.
  
  
  RESULTS
  All animals survived without any indications of injury. Although the employed solutions differed in their respective composition, comparable results in all plasma acid-base and electrolyte parameters studied were obtained. Plasma pH increased from approximately 7.28 to 7.39, the plasma K(+) concentration decreased from circa 5.20 mM to 4.80-3.90 mM and the plasma Cl(-) concentration rose from approximately 105 mM to 111-120 mM. Urinary analysis revealed increased excretion of K(+), H(+) and Cl(-).
  
  
  CONCLUSIONS
  The present data suggest that the carrier solution's composition with regard to metabolizable anions as well as K(+), Ca(2+) only has a minor impact on acid-base and electrolyte status after application of succinylated gelatin or HES-containing colloidal volume replacement solutions.
authors.affiliation: University of Duisburg-Essen, Institute of Physiological Chemistry, University Hospital Essen, Essen, Germany.
authors.forename: Johanna K
authors.initials: JK
authors.lastname: Teloh
authors.forename: Katja B
authors.initials: KB
authors.lastname: Ferenz
authors.forename: Frank
authors.initials: F
authors.lastname: Petrat
authors.forename: Christian
authors.initials: C
authors.lastname: Mayer
authors.forename: Herbert
authors.initials: H
authors.lastname: de Groot
mesh_terms: D000818:Animals; D004573:Electrolytes; D005780:Gelatin / Q000627:therapeutic use*; D006438:Hemodilution / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013386:Succinates / Q000627:therapeutic use*
chemical_list: D004573:Electrolytes; D013386:Succinates; C060085:succinylated gelatin; D005780:Gelatin
references.citation: Lancet. 1993 Jul 24;342(8865):248-9
references.pmid: 7686994
references.citation: Am J Physiol Renal Physiol. 2007 Feb;292(2):F796-803
references.pmid: 17077389
references.citation: Am J Physiol. 1965 Jan;208:149-53
references.pmid: 14253139
references.citation: Shock. 2007 Apr;27(4):390-6
references.pmid: 17414421
references.citation: Resuscitation. 2012 Sep;83(9):1166-72
references.pmid: 22353638
references.citation: Br J Anaesth. 2010 Sep;105(3):273-81
references.pmid: 20659913
references.citation: Crit Care Med. 1999 Jan;27(1):200-10
references.pmid: 9934917
references.citation: Am J Kidney Dis. 2008 Mar;51(3):491-503
references.pmid: 18295066
references.citation: Am J Vet Res. 1999 Dec;60(12):1577-83
references.pmid: 10622172
references.citation: Virchows Arch B Cell Pathol. 1968;1(3):240-50
references.pmid: 4971255
references.citation: Anesth Analg. 2008 Apr;106(4):1078-86, table of contents
references.pmid: 18349176
references.citation: Acta Chir Scand. 1968;134(6):481-7
references.pmid: 5731288
references.citation: J Cardiothorac Vasc Anesth. 2011 Jun;25(3):407-14
references.pmid: 21345699
references.citation: Br Med Bull. 1999;55(4):821-43
references.pmid: 10746333
references.citation: Anesthesiology. 2009 Jul;111(1):187-202
references.pmid: 19512862
references.citation: Indian J Anaesth. 2009 Oct;53(5):592-607
references.pmid: 20640110
references.citation: Acta Anaesthesiol Scand. 2010 Nov;54(10):1241-7
references.pmid: 20840513
references.citation: Am J Physiol. 1954 Jun;177(3):455-62
references.pmid: 13158595
references.citation: Br J Anaesth. 1996 Apr;76(4):519-25
references.pmid: 8652324
references.citation: Kidney Int. 2003 Sep;64(3):1046-9
references.pmid: 12911555
references.citation: Crit Care Med. 1989 Oct;17(10):996-8
references.pmid: 2791585
references.citation: Anesthesiol Clin. 2011 Sep;29(3):547-55
references.pmid: 21871410
references.citation: Wien Klin Wochenschr. 2004 Sep 30;116(17-18):583-94
references.pmid: 15515874
references.citation: Methods Find Exp Clin Pharmacol. 1987 Nov;9(11):717-20
references.pmid: 3448450
references.citation: Semin Thromb Hemost. 2007 Nov;33(8):810-5
references.pmid: 18175286
references.citation: Intensive Care Med. 2007 Feb;33(2):293-9
references.pmid: 17119921
references.citation: Krankenpfl J. 1997 Dec;35(12):497-506
references.pmid: 9470689
references.citation: Shock. 2012 Feb;37(2):205-9
references.pmid: 22089195
references.citation: Neurocrit Care. 2013 Feb;18(1):5-12
references.pmid: 22872427
references.citation: Proc Natl Acad Sci U S A. 1987 Mar;84(5):1464-8
references.pmid: 3029782
references.citation: Anesthesiology. 2000 Nov;93(5):1174-83
references.pmid: 11046202
references.citation: PLoS One. 2008 Apr 23;3(4):e2010
references.pmid: 18431491
references.citation: Anesth Analg. 1995 Dec;81(6):1235-42
references.pmid: 7486110
references.citation: Cochrane Database Syst Rev. 2012 Jun 13;(6):CD000567
references.pmid: 22696320
references.citation: Transplant Proc. 1998 Sep;30(6):2796-7
references.pmid: 9745568
references.citation: Hypertension. 1982 Jan-Feb;4(1):161-6
references.pmid: 7061123
references.citation: Crit Care Med. 2010 Feb;38(2):464-70
references.pmid: 19789444