title: Label-free nanometer-resolution imaging of biological architectures through surface enhanced Raman scattering.
abstract: Label free imaging of the chemical environment of biological specimens would readily bridge the supramolecular and the cellular scales, if a chemical fingerprint technique such as Raman scattering can be coupled with super resolution imaging. We demonstrate the possibility of label-free super-resolution Raman imaging, by applying stochastic reconstruction to temporal fluctuations of the surface enhanced Raman scattering (SERS) signal which originate from biomolecular layers on large-area plasmonic surfaces with a high and uniform hot-spot density (>10¹¹/cm², 20 to 35 nm spacing). A resolution of 20 nm is demonstrated in reconstructed images of self-assembled peptide network and fibrilated lamellipodia of cardiomyocytes. Blink rate density is observed to be proportional to the excitation intensity and at high excitation densities (>10 kW/cm²) blinking is accompanied by molecular breakdown. However, at low powers, simultaneous Raman measurements show that SERS can provide sufficient blink rates required for image reconstruction without completely damaging the chemical structure.
authors.affiliation: 1] UNAM Institute of Materials Science and Nanotechnology, Bilkent University, 06800 Ankara, Turkey [2].
authors.forename: Sencer
authors.initials: S
authors.lastname: Ayas
authors.forename: Goksu
authors.initials: G
authors.lastname: Cinar
authors.forename: Alper Devrim
authors.initials: AD
authors.lastname: Ozkan
authors.forename: Zeliha
authors.initials: Z
authors.lastname: Soran
authors.forename: Oner
authors.initials: O
authors.lastname: Ekiz
authors.forename: Deniz
authors.initials: D
authors.lastname: Kocaay
authors.forename: Aysel
authors.initials: A
authors.lastname: Tomak
authors.forename: Pelin
authors.initials: P
authors.lastname: Toren
authors.forename: Yasin
authors.initials: Y
authors.lastname: Kaya
authors.forename: Ilknur
authors.initials: I
authors.lastname: Tunc
authors.forename: Hadi
authors.initials: H
authors.lastname: Zareie
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authors.initials: T
authors.lastname: Tekinay
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authors.initials: AB
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authors.forename: Mustafa Ozgur
authors.initials: MO
authors.lastname: Guler
authors.forename: Aykutlu
authors.initials: A
authors.lastname: Dana
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