title: Human H7N9 influenza A viruses replicate in swine respiratory tissue explants.
abstract: Recently, novel H7N9 influenza viruses have caused an unprecedented outbreak in humans. Pigs are an important intermediate host for influenza; thus, we assessed the replication ability of three human H7N9 viruses (A/Anhui/1/2013, A/Shanghai/1/2013, A/Shanghai/2/2013) in swine tissue explants. All viruses tested replicated efficiently in explants from tracheas and bronchi, with limited replication in alveolar cells. Swine respiratory tissue explants can serve as an efficient model for screening replication potential of newly emerging H7N9 viruses.
authors.affiliation: Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Jones
authors.forename: T
authors.initials: T
authors.lastname: Baranovich
authors.forename: H
authors.initials: H
authors.lastname: Zaraket
authors.forename: Y
authors.initials: Y
authors.lastname: Guan
authors.forename: Y
authors.initials: Y
authors.lastname: Shu
authors.forename: R J
authors.initials: RJ
authors.lastname: Webby
authors.forename: R G
authors.initials: RG
authors.lastname: Webster
mesh_terms: D000818:Animals; D001980:Bronchi / Q000378:metabolism / Q000821:virology*; D006801:Humans; D007124:Immunoenzyme Techniques; D064766:Influenza A Virus, H7N9 Subtype / Q000472:pathogenicity*; D009976:Orthomyxoviridae Infections / Q000378:metabolism / Q000821:virology*; D011650:Pulmonary Alveoli / Q000378:metabolism / Q000821:virology*; D013552:Swine; D013556:Swine, Miniature; D046509:Tissue Culture Techniques; D014132:Trachea / Q000378:metabolism / Q000821:virology*
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