title: Adoptive immunotherapy with antigen-specific T cells during extracorporeal membrane oxygenation (ECMO) for adenovirus-related respiratory failure in a child given haploidentical stem cell transplantation.
abstract: We report on the successful infusion of human adenovirus (HAdV)-specific T cells in a child with congenital amegakaryocytic thrombocytopenia, given T-cell-depleted hematopoietic stem cell transplantation (HSCT) from the HLA-haploidentical mother during extracorporeal membrane oxygenation (ECMO) for severe HAdV-related respiratory failure. Donor-derived, interferon (IFN)-γ-secreting HAdV-specific T cells were enriched using the cytokine capture assay, after in vitro stimulation with overlapping peptides from the immunodominant HAdV5 hexon protein. Two weeks after T-cell transfer, viral load decreased and ECMO was discontinued. T-cell responses to HAdV antigens were documented after four weeks and were associated with viral clearance, immune reconstitution and clinical amelioration.
authors.affiliation: Department of Pediatric Intensive Care, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Di Nardo
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Li Pira
authors.forename: Antonio
authors.initials: A
authors.lastname: Amodeo
authors.forename: Corrado
authors.initials: C
authors.lastname: Cecchetti
authors.forename: Ezio
authors.initials: E
authors.lastname: Giorda
authors.forename: Stefano
authors.initials: S
authors.lastname: Ceccarelli
authors.forename: Letizia P
authors.initials: LP
authors.lastname: Brescia
authors.forename: Nicola
authors.initials: N
authors.lastname: Pirozzi
authors.forename: Sergio
authors.initials: S
authors.lastname: Rutella
authors.forename: Franco
authors.initials: F
authors.lastname: Locatelli
authors.forename: Alice
authors.initials: A
authors.lastname: Bertaina
mesh_terms: D000258:Adenovirus Infections, Human / Q000209:etiology / Q000628:therapy*; D000260:Adenoviruses, Human / Q000276:immunology; D036022:Capsid Proteins / Q000276:immunology*; D002648:Child; D000080984:Congenital Bone Marrow Failure Syndromes; D015199:Extracorporeal Membrane Oxygenation; D006801:Humans; D016219:Immunotherapy, Adoptive; D007371:Interferon-gamma / Q000378:metabolism; D008212:Lymphocyte Depletion; D008297:Male; D010446:Peptide Fragments / Q000276:immunology; D011379:Prognosis; D012128:Respiratory Distress Syndrome / Q000209:etiology / Q000628:therapy*; D033581:Stem Cell Transplantation / Q000009:adverse effects*; D013601:T-Lymphocytes / Q000276:immunology* / Q000821:virology; D013921:Thrombocytopenia / Q000150:complications* / Q000276:immunology / Q000628:therapy; D014184:Transplantation, Homologous; D019562:Viral Load
chemical_list: D036022:Capsid Proteins; D010446:Peptide Fragments; C018315:hexon capsid protein, Adenovirus; D007371:Interferon-gamma
keywords: ECMO; T lymphocyte; adenovirus; immune recovery; immunotherapy; stem cell transplantation