title: Synthesis, molecular docking and biological evaluation of new steroidal 4H-pyrans.
abstract: A series of new steroidal 4H-pyrans (4-6) have been synthesized from steroidal α, β-unsaturated ketones (1-3). The products (4-6) were characterized by IR, (1)H NMR, (13)C NMR, MS and analytical data. The interaction studies of compounds (4-6) with DNA were carried out by employing gel electrophoresis, UV-vis and fluorescence spectroscopy. The gel electrophoresis pattern revealed that compounds (4-6) bind to DNA and also demonstrated that the compound 6 alone or in presence of Cu (II) causes the nicking of supercoiled pBR322. The compounds 4 and 5 bind to DNA preferentially through electrostatic and hydrophobic interactions with Kb values found to be 5.3×10(3) and 3.7×10(3) M(-1), respectively, indicating the higher binding affinity of compound 4 towards DNA. The docking study suggested the intercalation of compounds in between the nucleotide base pairs. The cytotoxicity and genotoxicity of the newly synthesized compounds were checked by MTT and comet assay, respectively during which compound 6 showed potential behaviour.
authors.affiliation: Department of Chemistry, Aligarh Muslim University, Aligarh 202 002, India. Electronic address: shamsuzzaman9@gmail.com.
authors.forename: Shams
authors.initials: S
authors.lastname: Uzzaman
authors.forename: Ayaz Mahmood
authors.initials: AM
authors.lastname: Dar
authors.forename: Aamir
authors.initials: A
authors.lastname: Sohail
authors.forename: Sheraz
authors.initials: S
authors.lastname: Bhat
authors.forename: Mir Faisal
authors.initials: MF
authors.lastname: mustafa
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Khan
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D020552:Comet Assay; D003300:Copper / Q000737:chemistry; D004247:DNA / Q000235:genetics*; D004249:DNA Damage / Q000187:drug effects; D017665:Hydroxyl Radical / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009369:Neoplasms / Q000188:drug therapy / Q000473:pathology; D011714:Pyrans / Q000737:chemistry / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D021241:Spectrometry, Mass, Electrospray Ionization; D013055:Spectrophotometry, Infrared; D013256:Steroids / Q000737:chemistry / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D011714:Pyrans; D013256:Steroids; D017665:Hydroxyl Radical; D003300:Copper; D004247:DNA
keywords: 4H-pyran; Anticancer; Comet assay; Docking; Ethyl cyanoacetate