title: New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli.
abstract: We report the construction of two vectors for Escherichia coli: pUC72, for molecular cloning, and pPLT7, for thermal-induced expression. The main feature of pUC72 is a novel polylinker region that includes restriction sites for Nde I and Nco I which provide an ATG codon for proper translation initiation of expressed genes. Vector pPLT7 is ideal for thermo-inducible expression in host cells that carry the cI857 repressor gene. The use of pPLT7 was validated by the successful expression of the genes encoding carp and porcine growth hormones. These vectors provide novel cloning possibilities in addition to simple, non-expensive, high level expression of recombinant proteins in E. coli.
authors.affiliation: Universidade de Brasília , Brasília, DF , Brasil.
authors.forename: Mauro Aparecido Souza
authors.initials: MA
authors.lastname: Xavier
authors.forename: André
authors.initials: A
authors.lastname: Kipnis
authors.forename: Fernando Araripe Gonçalves
authors.initials: FA
authors.lastname: Torres
authors.forename: Spartaco
authors.initials: S
authors.lastname: Astofi-Filho
keywords: Escherichia coli; Heterologous expression; induced expression; molecular cloning
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