title: Three-dimensional microscaffolds exhibiting spatially resolved surface chemistry.
abstract: Spatial control over the surface chemistry of 3D organic-inorganic hybrid microscaffolds is achieved by a two-photon-triggered cycloaddition. Following a coating step with photoactivatable dienes via silanization, surface irradiation with a femtosecond-pulsed laser in the presence of functional dienophiles enables a site-selective alteration of the surface chemistry. Bioconjugation with fluorescent protein tags is employed to reveal the 3D molecular patterns.
authors.affiliation: Cell- and Neurobiology, Zoological Institute, Haid-und-Neu-Strasse 9, 76131 Karlsruhe, Germany and Institute of Functional Interfaces (IFG), Karlsruhe Institute of Technology (KIT) Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Richter
authors.forename: Thomas
authors.initials: T
authors.lastname: Pauloehrl
authors.forename: Johannes
authors.initials: J
authors.lastname: Kaschke
authors.forename: Dagmar
authors.initials: D
authors.lastname: Fichtner
authors.forename: Joachim
authors.initials: J
authors.lastname: Fischer
authors.forename: Alexandra M
authors.initials: AM
authors.lastname: Greiner
authors.forename: Doris
authors.initials: D
authors.lastname: Wedlich
authors.forename: Martin
authors.initials: M
authors.lastname: Wegener
authors.forename: Guillaume
authors.initials: G
authors.lastname: Delaittre
authors.forename: Christopher
authors.initials: C
authors.lastname: Barner-Kowollik
authors.forename: Martin
authors.initials: M
authors.lastname: Bastmeyer
keywords: Diels-Alder cycloaddition; direct laser writing; photopatterning; protein conjugation; three-dimension