title: Wnt1 Accelerates an Ex Vivo Expansion of Human Cord Blood CD34(+)CD38(-) Cells.
abstract: Cord blood hematopoietic stem cells (CB-HSCs) transplantation has been increasing gradually with facing the limitation of insufficient quantity of HSCs in each CB unit. Therefore, efficient expansion methods which can maintain stem cell characteristics are needed. In this study, umbilical CB-CD34(+) cells were cultured in two different cytokine cocktails: 4 factors (4F = Flt3-L, SCF, IL-6, and TPO) and 5 factors (5F = Wnt1 + 4F) in both serum and serum-free media. The data revealed that the best condition to accelerate an expansion of CD34(+)CD38(-) cells was serum-free culture condition supplemented with 5F (5F KSR). This condition yielded 24.3 ± 2.1 folds increase of CD34(+)CD38(-) cells. The expanded cells exhibited CD34(+) CD38(-) CD133(+) CD71(low) CD33(low) CD3(-) CD19(-) markers, expressed nanog, oct3/4, c-myc, and sox2 genes, and maintained differentiation potential into lymphoid, erythroid and myeloid lineages. The achievement of CD34(+)CD38(-) cells expansion may overcome an insufficient quantity of the cells leading to the improvement of the stem cell transplantation. Altogether, our findings highlight the role of Wnt1 and the new culture condition in stimulating hematopoietic stem/progenitor cells expansion which may offer a new therapeutic avenue for cord blood transplantation, regenerative medicine, stem cell bank applications, and other clinical applications in the future.
authors.affiliation: Stem Cell Therapy and Transplantation Research Group, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand ; School of Microbiology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
authors.forename: Kamonnaree
authors.initials: K
authors.lastname: Chotinantakul
authors.forename: Patcharee
authors.initials: P
authors.lastname: Prasajak
authors.forename: Wilairat
authors.initials: W
authors.lastname: Leeanansaksiri
references.citation: Stem Cells Dev. 2009 Sep;18(7):1093-108
references.pmid: 19480567
references.citation: Pancreas. 2008 Mar;36(2):e30-5
references.pmid: 18376297
references.citation: Eur J Haematol. 2009 Feb;82(2):124-32
references.pmid: 19067743
references.citation: FASEB J. 2006 May;20(7):950-2
references.pmid: 16585061
references.citation: Stem Cells. 2009 Aug;27(8):1858-68
references.pmid: 19544418
references.citation: J Clin Invest. 2000 Apr;105(7):1013-21
references.pmid: 10749580
references.citation: Blood. 2010 Jun 17;115(24):5012-22
references.pmid: 20357242
references.citation: Blood. 2009 Mar 12;113(11):2410-5
references.pmid: 18997171
references.citation: Proc Natl Acad Sci U S A. 1997 May 13;94(10):5320-5
references.pmid: 9144235
references.citation: Exp Hematol. 2010 Jul;38(7):593-602
references.pmid: 20417683
references.citation: Blood. 2009 Jan 15;113(3):546-54
references.pmid: 18832654
references.citation: Blood. 2004 Jun 15;103(12):4440-8
references.pmid: 14726371
references.citation: Nat Immunol. 2005 Mar;6(3):314-22
references.pmid: 15665828
references.citation: Cell Biol Int. 2010 Aug;34(8):783-90
references.pmid: 20397976
references.citation: Exp Mol Med. 2010 Feb 28;42(2):122-31
references.pmid: 20054234
references.citation: Blood. 2008 Jan 1;111(1):122-31
references.pmid: 17875805
references.citation: Biol Blood Marrow Transplant. 2002;8(7):368-76
references.pmid: 12171483
references.citation: PLoS One. 2010 Aug 17;5(8):e12221
references.pmid: 20808921
references.citation: J Transl Med. 2009 Jan 14;7:6
references.pmid: 19144182
references.citation: Nat Med. 2010 Feb;16(2):232-6
references.pmid: 20081862
references.citation: Acta Paediatr Jpn. 1994 Dec;36(6):649-55
references.pmid: 7871975
references.citation: Stem Cells. 2010 Aug;28(8):1379-89
references.pmid: 20521329
references.citation: Blood. 1997 Apr 15;89(8):2644-53
references.pmid: 9108381
references.citation: Nat Med. 2004 Jan;10(1):55-63
references.pmid: 14702635
references.citation: Blood. 1995 Nov 15;86(10):3745-53
references.pmid: 7579341
references.citation: Differentiation. 2007 Feb;75(2):100-11
references.pmid: 17316380
references.citation: Nature. 2003 May 22;423(6938):409-14
references.pmid: 12717450
references.citation: Circ Res. 2003 Sep 5;93(5):e51-62
references.pmid: 12919944
references.citation: Eur J Haematol. 2010 Jun;84(6):538-46
references.pmid: 20088916
references.citation: Nat Cell Biol. 2007 Jun;9(6):625-35
references.pmid: 17515932
references.citation: Exp Hematol. 2009 Sep;37(9):1108-20, 1120.e1-4
references.pmid: 19576951
references.citation: Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3422-7
references.pmid: 12626754
references.citation: Ann Hematol. 2011 Feb;90(2):127-37
references.pmid: 20821012
references.citation: Blood. 1997 May 15;89(10):3624-35
references.pmid: 9160667
references.citation: Bone Marrow Transplant. 2009 Apr;43(8):627-35
references.pmid: 18997827
references.citation: J Hematother Stem Cell Res. 2000 Dec;9(6):945-56
references.pmid: 11177609
references.citation: Proc Natl Acad Sci U S A. 2006 May 2;103(18):6907-12
references.pmid: 16632596
references.citation: Leuk Lymphoma. 2010 Jun;51(6):975-82
references.pmid: 20367131
references.citation: Methods Mol Biol. 2007;407:177-208
references.pmid: 18453257
references.citation: N Engl J Med. 1996 Dec 12;335(24):1839-40
references.pmid: 8943169
references.citation: Blood. 2000 Nov 1;96(9):3001-7
references.pmid: 11049977
references.citation: Stem Cells. 2007 Feb;25(2):500-10
references.pmid: 17068183
references.citation: Cytotechnology. 2011 May;63(3):259-68
references.pmid: 21327936
references.citation: Exp Neurol. 2006 Feb;197(2):399-406
references.pmid: 16298364
references.citation: Transfus Apher Sci. 2010 Jun;42(3):265-75
references.pmid: 20427240
references.citation: Cell. 2003 May 30;113(5):643-55
references.pmid: 12787505
references.citation: Blood. 2010 Jan 14;115(2):257-60
references.pmid: 19897585