title: Utilization of dibenzothiophene as sulfur source by Microbacterium sp. NISOC-06.
abstract: Oil-polluted soils were sampled from National Iranian South Oil Company (NISOC) for isolation and screening of C-S and not C-C targeted Dibenzothiophene (DBT) degrading microorganisms. Microbacterium sp. NISOC-06, a C-S targeted DBT degrading bacterium, was selected and its desulfurization ability was studied in aqueous phase and water-gasoline biphasic systems. The 16srRNA gene was amplified using universal eubacteria-specific primers, PCR product was sequenced and the sequence of nearly 1,500 bp 16srDNA was studied. Based on Gas Chromatography results Microbacterium sp. NISOC-06 utilized 94.8% of 1 mM DBT during the 2 weeks of incubation. UV Spectrophotometry and biomass production measurements showed that the Microbacterium sp. NISOC-06 was not able to utilize DBT as a carbon source. There was no accumulation of phenolic compounds as Gibb's assay showed. Biomass production in a biphasic system for which DBT-enriched gasoline was used as the sulfur source indicated the capability of Microbacterium sp. NISOC-06 to desulfurize gasoline.
authors.affiliation: Department of Biology, Faculty of Sciences, Shahid Chamran University, Ahvaz, Iran, moslempapizadeh@yahoo.com.
authors.forename: Moslem
authors.initials: M
authors.lastname: Papizadeh
authors.forename: Mohammad Roayaei
authors.initials: MR
authors.lastname: Ardakani
authors.forename: Gholamhossein
authors.initials: G
authors.lastname: Ebrahimipour
authors.forename: Hossein
authors.initials: H
authors.lastname: Motamedi
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