title: A synthetic riboswitch that operates using a rationally designed ligand-RNA pair.
abstract: The construction of an artificial riboswitch is based on a ligand-RNA pair without any molecular biology-based selection processes. The ligand selectively and significantly stabilized an RNA duplex containing an r(XGG)/r(XGG) sequence (X=U, A, G). The integration of the ligand-binding sequences into the 5'-untranslated region of mRNA provided an artificial riboswitch that was responsive to Z-NCTS.
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan); PRESTO (Japan) Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama 332-0012 (Japan). cdohno@sanken.osaka-u.ac.jp.
authors.forename: Chikara
authors.initials: C
authors.lastname: Dohno
authors.forename: Izumi
authors.initials: I
authors.lastname: Kohyama
authors.forename: Maki
authors.initials: M
authors.lastname: Kimura
authors.forename: Masaki
authors.initials: M
authors.lastname: Hagihara
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakatani
mesh_terms: D008024:Ligands; D015394:Molecular Structure; D009287:Naphthyridines / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D058928:Riboswitch
chemical_list: D008024:Ligands; D009287:Naphthyridines; D058928:Riboswitch; D012313:RNA
keywords: RNA-binding ligand; naphthyridine; riboswitches; synthetic ligands; translational regulation