title: Altered white adipose tissue protein profile in C57BL/6J mice displaying delipidative, inflammatory, and browning characteristics after bitter melon seed oil treatment.
abstract: OBJECTIVE
  We have previously shown that bitter melon seed oil (BMSO), which is rich in cis-9, trans-11, trans-13 conjugated linolenic acid, is more potent than soybean oil in attenuating body fat deposition in high-fat diet-induced obese C57BL/6J mice. The aim of this study was to obtain a comprehensive insight into how white adipose tissue (WAT) is affected by BMSO administration and to explore the underlying mechanisms of the anti-adiposity effect of BMSO.
  
  
  RESULTS
  A proteomic approach was used to identify proteins differentially expressed in the WAT of mice fed diets with or without BMSO for 11 wks. The WAT was also analyzed histologically for morphological changes. Two-dimensional gel electrophoresis (pH 4-7) revealed 32 spots showing a statistically significant difference (P<0.05) in intensity in BMSO-treated mice and 30 of these were shown to code for 23 proteins (15 increased and 8 decreased expression; >2-fold change). Combined with histological evidence of macrophage infiltration and brown adipocyte recruitment, the proteomic and immunoblotting data showed that the WAT in mice subjected to long-term high dose BMSO administration was characterized by reduced caveolae formation, increased ROS insult, tissue remodeling/repair, mitochondria uncoupling, and stabilization of the actin cytoskeleton, this last change being putatively related to an increased inflammatory response.
  
  
  CONCLUSIONS
  The anti-adiposity effect of BMSO is associated with WAT delipidation, inflammation, and browning. Some novel proteins participating in these processes were identified. In addition, the BMSO-mediated WAT browning may account for the increased inflammation without causing adverse metabolic effects.
authors.affiliation: Institute of Nutrition, China Medical University, Taichung, Taiwan.
authors.forename: Cheng-Hsien
authors.initials: CH
authors.lastname: Hsieh
authors.forename: Gou-Chun
authors.initials: GC
authors.lastname: Chen
authors.forename: Pei-Hsuan
authors.initials: PH
authors.lastname: Chen
authors.forename: Ting-Feng
authors.initials: TF
authors.lastname: Wu
authors.forename: Pei-Min
authors.initials: PM
authors.lastname: Chao
mesh_terms: D052437:Adipocytes, Brown / Q000378:metabolism; D052436:Adipose Tissue, White / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D050154:Adiposity / Q000187:drug effects / Q000235:genetics; D000284:Administration, Oral; D000818:Animals; D005227:Fatty Acids / Q000378:metabolism; D015870:Gene Expression; D007249:Inflammation / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D008264:Macrophages / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D029775:Momordica / Q000737:chemistry*; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology*; D020543:Proteome; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*
chemical_list: D005227:Fatty Acids; D010938:Plant Oils; D020543:Proteome
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