title: Trafficking of gold nanorods in breast cancer cells: uptake, lysosome maturation, and elimination.
abstract: Gold nanorods (AuNRs) have been largely investigated driven by their promising potentials in drug delivery, imaging, and photodynamic therapy because of their distinctive physicochemical properties. It is widely known that AuNRs can be taken up by different cells, however, the trafficking of the nanorods in cells are less known. In this work, the behaviors and fate of AuNRs in the human breast cancer cell line MDA-MB-231 were intensively probed by transmission electron microscopy (TEM) with detailed time resolution, together with induced couple plasmon mass spectroscopy (ICP-MS), confocal microscopy, Western blot, and cell viability assay. We reveal that AuNRs enter the classic lysosome maturation through endocytosis and are sequestered in the vesicular system even during cell division. AuNRs can escape from the lysosomes occasionally and the escaped AuNRs are recycled back into the lysosomal system through cytoprotective autophagy. The dilution of AuNRs in cells is mainly attributed to the cell division rather than exocytosis, because expelled AuNRs can be re-endocytosed by the cells. The feature of vesicular restriction guarantees other organelles such as mitochondria and nucleus are exempted from the direct exposure to AuNRs.
authors.affiliation: Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing 100005, P. R. China.
authors.forename: Weiqi
authors.initials: W
authors.lastname: Zhang
authors.forename: Yinglu
authors.initials: Y
authors.lastname: Ji
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Wu
authors.forename: Haiyan
authors.initials: H
authors.lastname: Xu
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems; D005089:Exocytosis / Q000187:drug effects; D005260:Female; D006046:Gold / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008247:Lysosomes / Q000737:chemistry / Q000187:drug effects; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D043942:Nanotubes / Q000009:adverse effects / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D006046:Gold