title: Molecular characterization of Verocytotoxigenic Escherichia coli O157:H7 isolates from Argentina by Multiple-Loci VNTR Analysis (MLVA).
abstract: The aim of this work was to adapt described MLVA protocols to the molecular typing and characterization of VTEC O157:H7 isolates from Argentina. Nine VNTR loci were amplified by PCR showing diversity values from 0.49 to 0.73. Nine MLVA profiles were observed and the cluster analysis indicated both unrelated and closely related VTEC O157:H7 strains. In spite of the limited number of isolates studied, the panel of VNTR used made it possible to perform a first approach of the high genetic diversity of native strains of O157:H7 by MLVA.
authors.affiliation: Laboratorio Inmunoquímica y Biotecnología, Departamento de Sanidad Animal y Medicina Preventiva, Faculdad Ciêcias Veterinarias , Universidad Nacional del Centro de la Pcia. de Buenos Aires , Tandil, Argentina.
authors.forename: Ana V
authors.initials: AV
authors.lastname: Bustamante
authors.forename: Paula M A
authors.initials: PM
authors.lastname: Lucchesi
authors.forename: Alberto E
authors.initials: AE
authors.lastname: Parma
keywords: MLVA; VTEC O157:H7; molecular characterization; typing
references.citation: Int Microbiol. 2004 Dec;7(4):269-76
references.pmid: 15666247
references.citation: J Clin Microbiol. 2006 Feb;44(2):374-7
references.pmid: 16455886
references.citation: Vet Res. 2005 May-Jun;36(3):289-311
references.pmid: 15845227
references.citation: J Clin Microbiol. 2006 Nov;44(11):3963-8
references.pmid: 16971640
references.citation: Medicina (B Aires). 2004;64(4):352-6
references.pmid: 15338982
references.citation: Electrophoresis. 2005 Jun;26(13):2567-82
references.pmid: 15937984
references.citation: J Appl Microbiol. 2005;98(4):928-40
references.pmid: 15752340
references.citation: Res Microbiol. 2006 Apr;157(3):263-6
references.pmid: 16171978
references.citation: Emerg Infect Dis. 2001 May-Jun;7(3):382-9
references.pmid: 11384513
references.citation: Int J Med Microbiol. 2005 Oct;295(6-7):405-18
references.pmid: 16238016
references.citation: Foodborne Pathog Dis. 2006 Spring;3(1):118-31
references.pmid: 16602987
references.citation: J Appl Microbiol. 2008 Nov;105(5):1344-53
references.pmid: 18631199
references.citation: Medicina (B Aires). 1998;58(1):1-7
references.pmid: 9674201
references.citation: Clin Microbiol Rev. 1998 Jul;11(3):450-79
references.pmid: 9665978
references.citation: BMC Vet Res. 2008 Jan 24;4:2
references.pmid: 18218128
references.citation: Clin Microbiol Rev. 1989 Jan;2(1):15-38
references.pmid: 2644022
references.citation: Bioinformatics. 2001 Dec;17(12):1230-1
references.pmid: 11751234
references.citation: J Med Microbiol. 2006 May;55(Pt 5):545-549
references.pmid: 16585641
references.citation: J Clin Microbiol. 2003 Dec;41(12):5389-97
references.pmid: 14662916
references.citation: Vet Microbiol. 2004 May 20;100(1-2):3-9
references.pmid: 15135507
references.citation: Ann Clin Microbiol Antimicrob. 2003 Dec 10;2:12
references.pmid: 14664722
references.citation: J Microbiol Methods. 2004 Aug;58(2):213-22
references.pmid: 15234519
references.citation: Microbiol Mol Biol Rev. 1998 Jun;62(2):275-93
references.pmid: 9618442