title: Pituitary adenylate cyclase activating peptide (PACAP) participates in adipogenesis by activating ERK signaling pathway.
abstract: Pituitary adenylate cyclase activating peptide (PACAP) belongs to the secretin/glucagon/vasoactive intestinal peptide (VIP) family. Its action can be mediated by three different receptor subtypes: PAC1, which has exclusive affinity for PACAP, and VPAC1 and VPAC2 which have equal affinity for PACAP and VIP. We showed that all three receptors are expressed in 3T3-L1 cells throughout their differentiation into adipocytes. We established the activity of these receptors by cAMP accumulation upon induction by PACAP. Together with insulin and dexamethasone, PACAP induced adipogenesis in 3T3-L1 cell line. PACAP increased cAMP production within 15 min upon stimulation and targeted the expression and phosphorylation of MAPK (ERK1/2), strengthened by the ERK1/2 phosphorylation being partially or completely abolished by different combinations of PACAP receptors antagonists. We therefore speculate that ERK1/2 activation is crucial for the activation of CCAAT/enhancer- binding protein β (C/EBPβ).
authors.affiliation: Laboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles, Brussels, Belgium.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Arsenijevic
authors.forename: Françoise
authors.initials: F
authors.lastname: Gregoire
authors.forename: Jeanne
authors.initials: J
authors.lastname: Chiadak
authors.forename: Elodie
authors.initials: E
authors.lastname: Courtequisse
authors.forename: Nargis
authors.initials: N
authors.lastname: Bolaky
authors.forename: Jason
authors.initials: J
authors.lastname: Perret
authors.forename: Christine
authors.initials: C
authors.lastname: Delporte
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D041721:3T3-L1 Cells; D050156:Adipogenesis; D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics / Q000378:metabolism; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005938:Glucocorticoids / Q000494:pharmacology; D007328:Insulin / Q000502:physiology; D020935:MAP Kinase Signaling System; D051379:Mice; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000502:physiology*; D051236:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: C495402:Adcyap1 protein, mouse; D000943:Antigens, Differentiation; D022782:CCAAT-Enhancer-Binding Protein-beta; D022762:CCAAT-Enhancer-Binding Proteins; C530718:CEBPA protein, mouse; C545013:Cebpb protein, mouse; D005938:Glucocorticoids; D007328:Insulin; D010726:Phosphodiesterase Inhibitors; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D051236:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; D003907:Dexamethasone; D000242:Cyclic AMP; D048049:Extracellular Signal-Regulated MAP Kinases; D015056:1-Methyl-3-isobutylxanthine
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