title: Downregulation of gas5 increases pancreatic cancer cell proliferation by regulating CDK6.
abstract: Recent studies have revealed that long non-coding RNAs (lncRNAs) play important roles in cancer biology and that lncRNA gas5 (growth arrest-specific 5) regulates breast cancer cell growth. However, the role of gas5 in pancreatic cancer progression remains largely unknown. In the current study, we assay the expression level of gas5 in pancreatic cancer tissues and define the role of gas5 in the regulation of pancreatic cancer cell proliferation. We verify that the expression level of gas5 is significantly decreased in pancreatic cancer tissues compared with normal control. Overexpression of gas5 in pancreatic cancer cells inhibits cell proliferation, whereas gas5 inhibition induces a significant decrease in G0/G1 phase and an increase in S phase. We further demonstrate that gas5 negatively regulates CDK6 (cyclin-dependent kinase 6) expression in vitro and in vivo. More importantly, knockdown of CDK6 partially abrogates gas5-siRNA-induced cell proliferation. These data suggest an important role of gas5 in the molecular etiology of pancreatic cancer and implicate the potential application of gas5 in pancreatic cancer therapy.
authors.affiliation: Department of General Surgery, RuiJin Hospital, Shanghai Jiaotong University School of Medicine, 197 RuiJin.Er.Road, ShangHai, 200025, China.
authors.forename: Xiongxiong
authors.initials: X
authors.lastname: Lu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Fang
authors.forename: Zhengting
authors.initials: Z
authors.lastname: Wang
authors.forename: Junjie
authors.initials: J
authors.lastname: Xie
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhan
authors.forename: Xiaxing
authors.initials: X
authors.lastname: Deng
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Jiabin
authors.initials: J
authors.lastname: Jin
authors.forename: Chenghong
authors.initials: C
authors.lastname: Peng
authors.forename: Hongwei
authors.initials: H
authors.lastname: Li
authors.forename: Baiyong
authors.initials: B
authors.lastname: Shen
mesh_terms: D000368:Aged; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D051361:Cyclin-Dependent Kinase 6 / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*
chemical_list: C561567:GAS5 long non-coding RNA, human; D062085:RNA, Long Noncoding; C495911:CDK6 protein, human; D051361:Cyclin-Dependent Kinase 6