title: In vitro and in vivo screening of anthelmintic activity of ginger and curcumin on Ascaridia galli.
abstract: Intestinal helmintic infection, continue to be a cause of major concern in several parts of the world, particularly in the developing nations. The use of plant extracts to control poultry helminths is increasing in different rearing systems. The anthelmintic activity of ginger and curcumin was studied on the nematode Ascaridia galli. In vitro and in vivo studies were allocated. Live parasites for in vitro studies were collected from the intestine of naturally infected chickens. Some living worms were incubated at 37 °C in media containing ginger at three concentration levels (25, 50, and 100 mg/ml), and others were incubated in media containing curcumin at the same concentration levels. Another living worm group was incubated in media containing albendazole at a dose of 7.5 mg/ml. The extracts' efficacy was exhibited in a concentration-time-dependent manner mainly at 100 mg/ml and after 48 h. The in vivo study takes place on experimentally infected chickens. Group of infected chickens was treated with ginger extract at dose of 100 mg, another group was treated with curcumin extract at dose of 100 mg, and a third group was treated with albendazole at dose of 7.5 mg. In vivo study of ginger and curcumin recorded lower mortality rates than the in vitro study. It is concluded that ginger and curcumin extracts have potential anthelmintic properties against A. galli. Ginger in all concentrations used exhibited a higher death rate observed than curcumin. Their wormicidal effect is concentration-time dependent.
authors.affiliation: Department of Pathology and Parasitology, Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt, ebazh@yahoo.com.
authors.forename: Eman K A
authors.initials: EK
authors.lastname: Bazh
authors.forename: Nasr M
authors.initials: NM
authors.lastname: El-Bahy
mesh_terms: D032761:Animal Experimentation; D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage* / Q000302:isolation & purification / Q000494:pharmacology*; D001197:Ascaridia / Q000187:drug effects* / Q000302:isolation & purification; D002645:Chickens; D030024:Curcuma / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D020939:Zingiber officinale / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000302:isolation & purification / Q000494:pharmacology*; D011201:Poultry Diseases / Q000188:drug therapy / Q000469:parasitology; D016019:Survival Analysis; D013997:Time Factors
chemical_list: D000871:Anthelmintics; D010936:Plant Extracts
references.citation: J Helminthol. 1987 Sep;61(3):247-52
references.pmid: 2822793
references.citation: Angew Parasitol. 1986 Aug;27(3):175-80
references.pmid: 3777528
references.citation: Parasitol Res. 2011 Apr;108(4):1041-6
references.pmid: 21120531
references.citation: Parasitol Res. 2011 Apr;108(4):1047-54
references.pmid: 21110041
references.citation: Planta Med. 2000 May;66(4):396-8
references.pmid: 10865470
references.citation: Parasitol Res. 2012 Dec;111(6):2467-71
references.pmid: 22773010
references.citation: Parasitol Res. 2013 May;112(5):1819-31
references.pmid: 23529336
references.citation: Vet Parasitol. 2012 Aug 13;188(1-2):78-84
references.pmid: 22463876
references.citation: Parasitol Res. 2010 Jul;107(2):261-9
references.pmid: 20461406
references.citation: Parasitol Res. 2010 Jan;106(2):395-401
references.pmid: 19898869
references.citation: Parasitol Res. 2011 Apr;108(4):879-86
references.pmid: 21057813
references.citation: Vet Parasitol. 2010 Aug 27;172(1-2):95-9
references.pmid: 20452126
references.citation: Parasitol Res. 2010 Apr;106(5):1233-9
references.pmid: 20191290
references.citation: Parasitol Res. 2011 Aug;109(2):395-403
references.pmid: 21327992
references.citation: Parasitol Res. 2011 Jan;108(1):177-86
references.pmid: 20865430
references.citation: Immunobiology. 2009;214(8):712-27
references.pmid: 19249123
references.citation: Parasitol Res. 2012 Feb;110(2):809-14
references.pmid: 21789584
references.citation: Trop Anim Health Prod. 2011 Feb;43(2):521-7
references.pmid: 21088894
references.citation: Curr Top Med Chem. 2002 May;2(5):439-56
references.pmid: 11966466
references.citation: Parasitol Res. 1996;82(1):32-7
references.pmid: 8825442
references.citation: Parasitol Res. 2011 Mar;108(3):513-9
references.pmid: 20922419
references.citation: Parasitol Res. 2011 Apr;108(4):979-84
references.pmid: 21107861
references.citation: J Ethnopharmacol. 2009 Jun 25;123(3):475-82
references.pmid: 19473794
references.citation: J Helminthol. 1987 Sep;61(3):268-70
references.pmid: 3668217
references.citation: Prev Vet Med. 2008 Jun 15;85(1-2):17-22
references.pmid: 18329115
references.citation: J Ethnopharmacol. 2007 May 30;112(1):7-12
references.pmid: 17329051
references.citation: Parasitol Res. 2009 Apr;104(5):1197-201
references.pmid: 19096877
references.citation: Antimicrob Agents Chemother. 2006 May;50(5):1859-60
references.pmid: 16641461
references.citation: J Ethnopharmacol. 2004 Aug;93(2-3):265-8
references.pmid: 15234763
references.citation: Phytother Res. 2006 Mar;20(3):187-90
references.pmid: 16521108
references.citation: Acta Trop. 2006 May;98(2):152-61
references.pmid: 16678115
references.citation: Parasitol Res. 1990;76(8):653-6
references.pmid: 2251240
references.citation: Anticancer Res. 2003 Sep-Oct;23(5A):3699-702
references.pmid: 14666666
references.citation: Indian J Exp Biol. 1990 Sep;28(9):871-5
references.pmid: 2279781
references.citation: Parasitol Res. 2012 Aug;111(2):881-8
references.pmid: 22526291
references.citation: J Biol Chem. 1988 Mar 5;263(7):3482-5
references.pmid: 2449442
references.citation: Parasitol Res. 2011 Jun;108(6):1557-63
references.pmid: 21153837
references.citation: Vet Parasitol. 2010 Jun 24;170(3-4):238-43
references.pmid: 20299150
references.citation: Parasitol Res. 2010 Jun;107(1):243-8
references.pmid: 20428889
references.citation: Parasitol Res. 2009 Nov;105(6):1483-7
references.pmid: 19774397
references.citation: Vet Parasitol. 1989 Aug 1;32(4):311-23
references.pmid: 2781717
references.citation: Parasitol Res. 2012 Jul;111(1):325-30
references.pmid: 22327318
references.citation: Phytother Res. 2005 Nov;19(11):988-91
references.pmid: 16317658
references.citation: J Ethnopharmacol. 2006 May 24;105(3):459-63
references.pmid: 16386396
references.citation: Parasitology. 2012 Mar;139(3):358-65
references.pmid: 22053741
references.citation: Parasitol Res. 2009 Oct;105(4):1155-61
references.pmid: 19557435
references.citation: Acta Trop. 2010 Jul-Aug;115(1-2):69-76
references.pmid: 20045669
references.citation: J Helminthol. 2002 Sep;76(3):241-7
references.pmid: 12363378
references.citation: J Ethnopharmacol. 2006 Jun 30;106(2):285-7
references.pmid: 16443342
references.citation: Vet Parasitol. 2003 Oct 8;116(2):159-73
references.pmid: 14519320