title: Effects of clinical isolates of Streptococcus pneumoniae on THP-1 human monocytic cells.
abstract: Twenty‑three clinical Streptococcus pneumoniae (SP) strains were isolated from blood and sputum specimens from the Second Affiliated Hospital of Wenzhou Medical College in 2009. These strains and the ATCC 49619 standard strain were cultured and suspended in normal saline (at a turbidity of 1.0 McFarland). The production of interleukin (IL)‑8, intracellular adhesion molecule‑1 (ICAM‑1) and IL‑10 in THP‑1 cells following stimulation with the SP suspension was analyzed by an enzyme-linked immunosorbent assay. The concentrations of IL‑8, ICAM‑1 and IL‑10 from the THP‑1 monocytes were greater than those of the blank control following stimulation with the SP suspension. No significant difference was identified in the levels of IL‑8, ICAM‑1 and IL‑10 secretion between THP‑1 monocytes stimulated by blood‑borne SP (bb‑SP) and sputum‑borne SP (sb‑SP).
authors.affiliation: Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, Zhejiang 318000, P.R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Da-Kang
authors.initials: DK
authors.lastname: Hu
authors.forename: Dong-Guo
authors.initials: DG
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Chi-Bo
authors.initials: CB
authors.lastname: Liu
authors.forename: Lian-Hua
authors.initials: LH
authors.lastname: Yu
authors.forename: Ying
authors.initials: Y
authors.lastname: Qu
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Luo
authors.forename: Jin-Hong
authors.initials: JH
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Yu
authors.forename: Shuang-Chun
authors.initials: SC
authors.lastname: Liu
authors.forename: Xiang-Yang
authors.initials: XY
authors.lastname: Li
mesh_terms: D019881:Aminoacyltransferases / Q000235:genetics; D002478:Cells, Cultured; D004269:DNA, Bacterial / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D016753:Interleukin-10 / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism* / Q000382:microbiology; D046915:Penicillin-Binding Proteins / Q000235:genetics; D011018:Pneumonia, Pneumococcal / Q000097:blood / Q000235:genetics / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction; D013183:Sputum / Q000378:metabolism* / Q000382:microbiology; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; C573420:ICAM1 protein, human; D016209:Interleukin-8; D046915:Penicillin-Binding Proteins; D018799:Intercellular Adhesion Molecule-1; D016753:Interleukin-10; D019881:Aminoacyltransferases; C478701:penicillin-binding protein 2b, Streptococcus