title: Diagnosis of paratuberculosis in cattle: microbiological culture, serology and PCR.
abstract: The aim of this study was to confirm clinical diagnosis of paratuberculosis in two cows showing suggestive clinical signs of the disease. Based on clinical signs, in culture and in IS900 PCR results from the individual milk samples it was possible to diagnose paratuberculosis in the cows studied.
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa , Viçosa, MG , Brasil.
authors.forename: Isabel A
authors.initials: IA
authors.lastname: Carvalho
authors.forename: Vinicius E B
authors.initials: VE
authors.lastname: Campos
authors.forename: Iana M
authors.initials: IM
authors.lastname: Souza
authors.forename: Larissa G
authors.initials: LG
authors.lastname: Zanardo
authors.forename: Jose D Ribeiro
authors.initials: JD
authors.lastname: Filho
authors.forename: Marcos J P
authors.initials: MJ
authors.lastname: Gomes
authors.forename: Maria A S
authors.initials: MA
authors.lastname: Moreira
keywords: IS900; ISMav2; Mycobacterium avium subspecies paratuberculosis (MAP); cattle; milk
references.citation: Appl Environ Microbiol. 2005 Jun;71(6):2853-61
references.pmid: 15932977
references.citation: J Vet Diagn Invest. 1997 Oct;9(4):375-80
references.pmid: 9376426
references.citation: Vet Microbiol. 2007 Nov 15;125(1-2):49-58
references.pmid: 17601689
references.citation: Vet Clin North Am Food Anim Pract. 1996 Jul;12(2):305-12
references.pmid: 8828107
references.citation: Mol Cell Probes. 1999 Dec;13(6):431-42
references.pmid: 10657148
references.citation: Nucleic Acids Res. 1989 Nov 25;17(22):9063-73
references.pmid: 2555783
references.citation: J Vet Diagn Invest. 2004 Mar;16(2):116-20
references.pmid: 15053361
references.citation: Vet Microbiol. 2005 Jul 1;108(3-4):263-70
references.pmid: 15885931
references.citation: Vet Res Commun. 2009 Oct;33(7):781-91
references.pmid: 19440851
references.citation: FEMS Microbiol Lett. 2001 Mar 1;196(1):31-7
references.pmid: 11257544
references.citation: J Dairy Sci. 2002 May;85(5):1052-7
references.pmid: 12086038
references.citation: Microbiology (Reading). 2000 Sep;146 ( Pt 9):2185-2197
references.pmid: 10974106
references.citation: J Dairy Sci. 2009 Nov;92(11):5408-10
references.pmid: 19841202
references.citation: J Clin Microbiol. 1992 May;30(5):1134-9
references.pmid: 1583110
references.citation: Indian J Exp Biol. 2007 Oct;45(10):843-52
references.pmid: 17948732
references.citation: J Vet Diagn Invest. 1999 Jul;11(4):345-51
references.pmid: 10424651
references.citation: Cornell Vet. 1984 Jul;74(3):218-62
references.pmid: 6375961
references.citation: J Dairy Sci. 2002 Nov;85(11):2795-802
references.pmid: 12487446