title: Serological responses to a soluble recombinant chimeric Plasmodium vivax circumsporozoite protein in VK210 and VK247 population.
abstract: BACKGROUND
  Circumsporozoite protein (CSP) is essential for sporozoite formation and sporozoite invasion into human hepatocyte. Previously, a recombinant P. vivax CSP based on chimeric repeats (rPvCSP-c) representing two major alleles VK210 and VK247 within central region has been designed. Naturally acquired humoral immune responses study show that antigenicity of rPvCSP-c was much higher than that of native strain. However, the serologic reactivity of rPvCSP-c was still unclear in detail.
  
  
  METHODS
  In present study, recognition of rPvCSP-c in vivax malaria typed VK210 and VK247 alleles was assessed. VK210 typed and VK247 typed sera from adult residents reacted specifically with rPvCSP-c using protein array and immunoblot assay. Additionally, anti-rPvCSP-c serum recognized the fixed VK210 and VK247 sporozoites by immunofluorescence assay. Furthermore, statistic analysis was performed for correlational detection.
  
  
  RESULTS
  The rPvCSP-c reacted with both VK210 typed and VK247 typed P. vivax infected patient sera and anti-rPvCSP-c immune serum also reacted with VK210 and VK247 sporozoite parasites of P. vivax specifically. There was a positive correlation between increased antibody level, age of patients and also associated with pvcsp repeat number, although the level of responses did vary considerably in their reactivity to the rPvCSP-c from negative to very high level within each age group.
  
  
  CONCLUSIONS
  These data confirmed the serologic reactivity of the novel rPvCSP-c in exposed both VK210 and VK247 populations. These results strongly suggested that this recombinant CSP was biologically active and potently immunogenic across major strains and raised the prospect that this protein could be used as serologic marker.
authors.affiliation: Department of Medical Environmental Biology and Tropical Medicine, Kangwon National University School of Medicine, Chuncheon, Gangwon-do 200-701, Republic of Korea. tsuboi@ccr.ehime-u.ac.jp.
authors.forename: Yang
authors.initials: Y
authors.lastname: Cheng
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ito
authors.forename: Jetsumon
authors.initials: J
authors.lastname: Sattabongkot
authors.forename: Chae Seung
authors.initials: CS
authors.lastname: Lim
authors.forename: Deok-Hoon
authors.initials: DH
authors.lastname: Kong
authors.forename: Kwon-Soo
authors.initials: KS
authors.lastname: Ha
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Takafumi
authors.initials: T
authors.lastname: Tsuboi
authors.forename: Eun-Taek
authors.initials: ET
authors.lastname: Han
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D015415:Biomarkers; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D008297:Male; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D010966:Plasmodium vivax / Q000235:genetics / Q000276:immunology*; D040081:Protein Array Analysis; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D055815:Young Adult
chemical_list: D000913:Antibodies, Protozoan; D015415:Biomarkers; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; C041595:circumsporozoite protein, Protozoan
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