title: Cell-nonautonomous inhibition of radiation-induced apoptosis by dynein light chain 1 in Caenorhabditis elegans.
abstract: The evolutionarily conserved process of programmed cell death, apoptosis, is essential for development of multicellular organisms and is also a protective mechanism against cellular damage. We have identified dynein light chain 1 (DLC-1) as a new regulator of germ cell apoptosis in Caenorhabditis elegans. The DLC-1 protein is highly conserved across species and is a part of the dynein motor complex. There is, however, increasing evidence for dynein-independent functions of DLC-1, and our data describe a novel dynein-independent role. In mammalian cells, DLC-1 is important for cellular transport, cell division and regulation of protein activity, and it has been implicated in cancer. In C. elegans, we find that knockdown of dlc-1 by RNA interference (RNAi) induces excessive apoptosis in the germline but not in somatic cells during development. We show that DLC-1 mediates apoptosis through the genes lin-35, egl-1 and ced-13, which are all involved in the response to ionising radiation (IR)-induced apoptosis. In accordance with this, we show that IR cannot further induce apoptosis in dlc-1(RNAi) animals. Furthermore, we find that DLC-1 is functioning cell nonautonomously through the same pathway as kri-1 in response to IR-induced apoptosis and that DLC-1 regulates the levels of KRI-1. Our results strengthen the notion of a highly dynamic communication between somatic cells and germ cells in regulating the apoptotic process.
authors.affiliation: Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10C, Aarhus C DK-8000, Denmark.
authors.forename: T H
authors.initials: TH
authors.lastname: Morthorst
authors.forename: A
authors.initials: A
authors.lastname: Olsen
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017209:Apoptosis / Q000528:radiation effects*; D017173:Caenorhabditis elegans / Q000166:cytology* / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D004398:Dyneins / Q000378:metabolism*; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008954:Models, Biological; D011839:Radiation, Ionizing
chemical_list: D029742:Caenorhabditis elegans Proteins; D049452:Green Fluorescent Proteins; C586892:DLC-1 protein, C elegans; D004398:Dyneins
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