title: Anti-Mycobacterium tuberculosis activity of fungus Phomopsis stipata.
abstract: Our purpose was to determine the anti-Mycobacterium tuberculosis activity of the metabolites produced by the endophitic fungus Phomopsis stipata (Lib.) B. Sutton, (Diaporthaceae), cultivated in different media. The antimycobacterial activity was assessed through the Resazurin Microtiter Assay (REMA) and the cytotoxicity test performed on macrophage cell line. The extracts derived from fungi grown on Corn Medium and Potato Dextrose Broth presented the smallest values of Minimum Inhibitory Concentration (MIC) and low cytotoxicity, which implies a high selectivity index. This is the first report on the chemical composition and antitubercular activity of metabolites of P. stipata, as well as the influence of culture medium on these properties.
authors.affiliation: Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista , Araraquara, SP , Brasil ; Faculdades Integradas Pitágoras , Montes Claros, MG , Brasil.
authors.forename: Karina Andrade
authors.initials: KA
authors.lastname: de Prince
authors.forename: Renata
authors.initials: R
authors.lastname: Sordi
authors.forename: Fernando Rogério
authors.initials: FR
authors.lastname: Pavan
authors.forename: Adolfo Carlos
authors.initials: AC
authors.lastname: Barreto Santos
authors.forename: Angela R
authors.initials: AR
authors.lastname: Araujo
authors.forename: Sergio R A
authors.initials: SR
authors.lastname: Leite
authors.forename: Clarice Q F
authors.initials: CQ
authors.lastname: Leite
keywords: Mycobacterium tuberculosis; Phomopsis stipata; antimycobacterial activity
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