title: Microcalorimetric study of the adsorption of lactoferrin in supermacroporous continuous cryogel with immobilized Cu(2+) ions.
abstract: The adsorption affinity of lactoferrin from whey in monolithic supermacroporous cryogel was analyzed using equilibrium data adsorptive isothermal titration microcalorimetry to measure thermodynamic information governing the process. Isotherm data was obtained at temperatures of 20, 30 and 40°C, pH 6, 7 and 8, and ionic strength of 200, 600 and 1000mmolL(-1) NaCl. The Langmuir model was fitted to equilibrium data. The binding was tighter at higher temperatures. The adsorption of protein was observed as spontaneous in all cases analyzed. The microcalorimetric study indicated that, in most cases examined, the adsorption of the protein in the matrix was entropy and enthalpy favored and entropy driven. Results provide data to enable the improvement of technical processes for the affinity separation of proteins.
authors.affiliation: Chemistry, Biotechnology and Bioprocess Department, Federal University of São João Del Rei - Ouro Branco, MG 36420-000, Brazil.
authors.forename: B M A
authors.initials: BM
authors.lastname: Carvalho
authors.forename: L H M
authors.initials: LH
authors.lastname: Da Silva
authors.forename: L M
authors.initials: LM
authors.lastname: Carvalho
authors.forename: A M
authors.initials: AM
authors.lastname: Soares
authors.forename: L A
authors.initials: LA
authors.lastname: Minim
authors.forename: S L
authors.initials: SL
authors.lastname: Da Silva
mesh_terms: D000327:Adsorption; D002151:Calorimetry / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods; D003300:Copper / Q000737:chemistry*; D059985:Cryogels / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007781:Lactoferrin / Q000737:chemistry* / Q000378:metabolism; D012965:Sodium Chloride / Q000737:chemistry; D013816:Thermodynamics
chemical_list: D059985:Cryogels; D012965:Sodium Chloride; D003300:Copper; D007781:Lactoferrin
keywords: Adsorption; Analytical biochemistry; Immobilized metal ion affinity chromatography; Lactoferrin; Microcalorimetry