[Role of miR-19a in ulcerative colitis in mice].
OBJECTIVE
To study the role of miR-19a in ulcerative colitis (UC) in mice.


METHODS
The target gene of miR-19a was predicted by bioinformatics analysis. The expression of the target protein in UC colon was detected by immunohistochemistry and Western blotting. The target gene was further identified by enhanced green fluorescent protein (EGFP) report vector system.


RESULTS
The target gene of miR-19a was TNF-α as predicted by bioinformatics analysis. TNF-α expression was highly expressed in the colonic tissue of UC mice. MiR-19a could inhibit the report gene activity of TNF-α-3'UTR-WT but no that of TNF-α-3'UTR-Mut.


CONCLUSIONS
The target gene of miR-19a is TNF-α, and the binding site is TNF-α 3'UTR. The possible role of miR-19a in UC pathogenesis involves regulation of TNF-α expression in the colon.
Department of Gastroenterology, First Affiliated Hospital, Sun Yat-sen University, Guangzhlu 510089, China.E-mail: pzzhoupz@126.com.
Pengzhi
P
Zhou
Bin
B
Chen
Pinjin
P
Hu
Yan
Y
Sun
D020413:3' Untranslated Regions; D000818:Animals; D001665:Binding Sites; D003093:Colitis, Ulcerative / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D007150:Immunohistochemistry; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D020413:3' Untranslated Regions; C561642:MIRN19 microRNA, mouse; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha