Screening for multiple weight loss and related drugs in dietary supplement materials by flow injection tandem mass spectrometry and their confirmation by liquid chromatography tandem mass spectrometry.
A new method has been developed using flow injection tandem mass spectrometry to semi-quantitatively screen for weight loss drugs, including sibutramine, N-desmethylsibutramine, N-didesmethylsibutramine, and phenolphthalein in dietary supplements. Positive identification of these drugs in samples was further confirmed and quantified by liquid chromatography tandem mass spectrometry. The degradation products of sibutramine were observed and identified by LC-MS/MS which include N-desmethylsibutramine, N-didesmethylsibutramine, N-formyldesmethylsibutramine, and N-formyldidesmethylsibutramine.
Food and Drug Administration, Method Development and Total Diet Research Center, 11630 W. 80th Street, Lenexa, KS 66214, United States. Electronic address: fenhong.song@fda.hhs.gov.
Fenhong
F
Song
Douglas
D
Monroe
Aref
A
El-Demerdash
Cynthia
C
Palmer
D019440:Anti-Obesity Agents / Q000032:analysis*; D002138:Calibration; D002623:Chemistry Techniques, Analytical; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D003503:Cyclobutanes / Q000032:analysis*; D019587:Dietary Supplements / Q000032:analysis*; D004340:Drug Contamination; D020113:Phenolphthalein / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
D019440:Anti-Obesity Agents; D003503:Cyclobutanes; D020113:Phenolphthalein; C058254:sibutramine
Dietary supplement; Flow injection; LC–MS/MS; Sibutramine; Weight loss drugs