Targeting PKCε by miR-143 regulates cell apoptosis in lung cancer.
Non-small cell lung cancer (NSCLC) is one of the most common causes for lung cancer and cancer-related death. The imbalance between cell proliferation and apoptosis was suggested to play an important role in cancer pathogenesis and PKCε is one of the widely recognized targets. Here, we demonstrate that miR-143 is aberrantly downregulated in NSCLC tissue and negatively correlates with expression of PKCε. We show that miR-143 specifically targets the 3'-UTR of PKCε and regulates its expression. Treatment with miR-143 inhibitor mimics cell proliferation and apoptosis imbalance in NSCLC, while inhibition of PKCε can reverse it. Our findings suggest that targeting PKCε overexpression in NSCLC should be beneficial for lung cancer therapy.
Department of Throracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Ni
N
Zhang
Yunshu
Y
Su
Lijun
L
Xu
D020413:3' Untranslated Regions; D000368:Aged; D017209:Apoptosis; D001483:Base Sequence; D001665:Binding Sites; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004789:Enzyme Activation; D004794:Enzyme Repression; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D051744:Protein Kinase C-epsilon / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference
D020413:3' Untranslated Regions; C542359:MIRN143 microRNA, human; D035683:MicroRNAs; D051744:Protein Kinase C-epsilon
NSCLC; PKCε; miR-143