Effects of interim acrylic resins on the expression of cytokines from epithelial cells and on collagen degradation.
BACKGROUND
Interim acrylic resins release agents that alter cytokine expression in the surrounding tissues, which could alter extracellular matrix degradation.


OBJECTIVE
The purpose of the study was to evaluate the responses of human epidermal keratinocytes to eluates of interim acrylic resins in regards to cytokine expression and cell-mediated collagen degradation.


METHODS
Specimens of 4 different interim acrylic resins (HI-I, Jet Acrylic, SNAP acrylic, and Protemp Plus) were placed in Epilife medium for 48 hours and the eluates collected. The cells were incubated for 72 hours in nontoxic concentrations of the eluates. Cytotoxicity was evaluated with lactate dehydrogenase assays and cytokine expression with cytokine antibody arrays. Collagen degradation was determined with a collagen type I assay. The experiments were performed 3 times. Data were analyzed with 1-way and mixed-model ANOVA (α=.05).


RESULTS
None of the eluates were cytotoxic. Cytokine expression from the heat-activated polymethyl methacrylate resin group was significantly less for interleukin-3, but significantly greater for interlukin-7. Expression for the chemically activated polymethyl methacrylate resin group was significantly less for growth-regulated oncogene-α, interleukin-1α, and interleukin-3. Expression for the chemically activated polyethyl methacrylate resin group was significantly less for interleukin-1α and interleukin-3, but significantly greater for interleukin-13 and monocytes chemoattractant protein-3. The cytokine expression induced by chemically activated bis-acryl composite resin was significantly greater for granulocyte-macrophage colony stimulating factor, interleukin-7, and monocytes chemoattractant protein-3, but significantly less for growth-regulated oncogene-α. Collagen degradation was not significantly different in any of the groups.


CONCLUSIONS
The eluates used were not cytotoxic and did not induce cell-mediated collagen degradation. Some significant changes in cytokine expression were noted.
Resident, Department of Restorative Dentistry, Indiana University School of Dentistry, Indiana University, Indianapolis, Ind; Implant fellow, Department of Restorative Dentistry and Biomaterials Sciences, Harvard School of Dental Medicine, Boston, Mass; Department of Substitutive Dental Sciences, College of Dentistry, Taibah University, Al Madinah Al Munawwarah, KSA.
Sary
S
Borzangy
Nawaf
N
Labban
L Jack
LJ
Windsor
D000180:Acrylic Resins / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D054410:Chemokine CCL7 / Q000032:analysis; D054360:Chemokine CXCL1 / Q000032:analysis; D003094:Collagen / Q000187:drug effects*; D024042:Collagen Type I / Q000032:analysis; D003188:Composite Resins / Q000737:chemistry / Q000494:pharmacology; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000187:drug effects*; D003764:Dental Materials / Q000737:chemistry / Q000494:pharmacology*; D016326:Extracellular Matrix Proteins / Q000187:drug effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis; D006801:Humans; D018793:Interleukin-13 / Q000032:analysis; D053582:Interleukin-1alpha / Q000032:analysis; D007377:Interleukin-3 / Q000032:analysis; D015851:Interleukin-7 / Q000032:analysis; D015603:Keratinocytes / Q000187:drug effects* / Q000276:immunology; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008768:Methylmethacrylates / Q000737:chemistry / Q000494:pharmacology; D011109:Polymethacrylic Acids / Q000737:chemistry / Q000494:pharmacology; D019904:Polymethyl Methacrylate / Q000737:chemistry / Q000494:pharmacology
D000180:Acrylic Resins; D054410:Chemokine CCL7; D054360:Chemokine CXCL1; D024042:Collagen Type I; D003188:Composite Resins; D017077:Culture Media, Conditioned; D016207:Cytokines; D003764:Dental Materials; D016326:Extracellular Matrix Proteins; C040292:Hi-I resin; C508596:IL3 protein, human; C508605:IL7 protein, human; D018793:Interleukin-13; D053582:Interleukin-1alpha; D007377:Interleukin-3; D015851:Interleukin-7; D008768:Methylmethacrylates; D011109:Polymethacrylic Acids; C091347:vinyl ethyl methacrylate; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; C009931:poly(ethylmethacrylate); D003094:Collagen; D019904:Polymethyl Methacrylate; C069714:Protemp; D007770:L-Lactate Dehydrogenase