Live intracellular super-resolution imaging using site-specific stains.
Point localization super-resolution imaging (SR) requires dyes that can cycle between fluorescent and dark states, in order for their molecular positions to be localized and create a reconstructed image. Dyes should also densely decorate biological features of interest to fully reveal structures being imaged. We tested site-specific dyes in several live-cell compatible imaging media and evaluated their performance in situ. We identify a number of new dyes and imaging medium-dye combinations for live staining, that densely highlight intracellular structures with excellent photophysical performance for SR.
Laboratory of Experimental Biophysics, École Polytechnique Fédérale de Lausanne , Lausanne, Switzerland.
Lina
L
Carlini
Suliana
S
Manley
D045744:Cell Line, Tumor; D003470:Culture Media / Q000737:chemistry; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006056:Golgi Apparatus / Q000648:ultrastructure; D006801:Humans; D008247:Lysosomes / Q000648:ultrastructure; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000648:ultrastructure; D057054:Molecular Imaging / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*
D003470:Culture Media; D005456:Fluorescent Dyes