Pregabalin determination in hair by ultra-high-performance liquid chromatography-tandem mass spectrometry.
Pregabalin is an antiepileptic and analgesic drug, commercialized under the name of Lyrica, and generally used to treat neuropathic pain. The determination of pregabalin was performed by using spiked hair samples extracted in methanol and analyzed by ultra-high-performance liquid chromatography coupled with tandem mass spectrometry. The validation of a quantification method of pregabalin in hair has been successfully achieved (precision, accuracy, matrix effects and extraction yield). The limit of detection was 0.76 pg/mg and the lower limit of quantification was 2.5 pg/mg. A real case of pregabalin in hair has been analyzed using this method. The result showed a concentration of 540 pg/mg.
Department of Toxicology, Laboratoire National de Santé, Avenue de la Faiencerie, 162 A, L-1511 Luxembourg, Luxembourg.
Caroline
C
Pauly
Michel
M
Yegles
Serge
S
Schneider
D000700:Analgesics / Q000032:analysis* / Q000302:isolation & purification; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006197:Hair / Q000737:chemistry*; D007202:Indicators and Reagents; D000069583:Pregabalin; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification
D000700:Analgesics; D007202:Indicators and Reagents; D000069583:Pregabalin; D005680:gamma-Aminobutyric Acid