Preparation and antibacterial activity of lysozyme and layered double hydroxide nanocomposites.
It is necessary to develop "green" disinfection technology which does not produce disinfection by-products. Lysozyme-layered double hydroxide nanocomposites (LYZ-LDHs) were prepared by intercalating LYZ in LDH for the first time. Their antibacterial activity was evaluated using staphylococcus aureus as a target. The bacteria removal mechanism was also studied. Characterization of LYZ-LDHs by X-ray diffraction and Fourier transform infrared spectroscopy indicated that LYZ was successfully intercalated in LDH, compressed and deformed without secondary structural change. LYZ-LDHs showed excellent bactericidal effectiveness against staphylococcus aureus. The antibacterial performance of LYZ-LDHs was found to be affected by the LYZ/LDH ratio and the pH of the bacteria-containing water. The bacteria removal efficiency of LYZ-LDHs with LYZ/LDH mass ratio of 0.8 was consistently above 94% over the pH range of 3-9. LYZ-LDHs adsorbed bacteria to their surface by LDH and then killed them by the immobilized LYZ. This new material integrated the bactericidal ability of LYZ and adsorption ability of LDH. Moreover, the antibacterial ability of LYZ-LDHs was persistent and not limited by the adsorption capacity.
Department of Environmental Engineering, Peking University, Beijing 100871, PR China; Shandong Provincial Key Laboratory of Microbial Engineering, College of Food and Bioengineering, Shandong Polytechnic University, Jinan 250353, PR China.
Qin-Zheng
QZ
Yang
Ying-Yue
YY
Chang
Hua-Zhang
HZ
Zhao
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002645:Chickens; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009113:Muramidase / Q000494:pharmacology*; D053761:Nanocomposites / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification; D055672:Static Electricity; D014961:X-Ray Diffraction
D000900:Anti-Bacterial Agents; D006878:Hydroxides; C031356:hydroxide ion; D009113:Muramidase
Disinfection; Intercalation; Layered double hydroxide; Lysozyme; Water purification