Determination of antiplasmodial activity and binding affinity of selected natural products towards PfTrxR and PfGR.
In our study, the binding affinities of selected natural products towards PfTrxR, PfGR, human TrxR and human GR were determined using a mass spectrometry based ligand binding assay. The in vitro antimalarial activity and cytotoxicity of these ligands were also determined. Catharanthine, 11-(OH)-coronaridine, hernagine, vobasine and hispolone displayed antiplasmodial activity against PfK1 (IC50 = 0.996-3.63 microg/mL).
Department of Pharmacal Sciences, 4306 Walker Building, Auburn University, Auburn, AL, USA.
Ranjith
R
Munigunti
Katja
K
Becker
Reto
R
Brun
Angela I
AI
Calderón
D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D001688:Biological Products / Q000494:pharmacology*; D005980:Glutathione Reductase / Q000037:antagonists & inhibitors*; D006801:Humans; D007050:Ibogaine / Q000031:analogs & derivatives / Q000494:pharmacology; D026121:Indole Alkaloids / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D010963:Plasmodium falciparum / Q000201:enzymology*; D013880:Thioredoxin-Disulfide Reductase / Q000037:antagonists & inhibitors*; D014748:Vinca Alkaloids / Q000494:pharmacology
D000981:Antiprotozoal Agents; D001688:Biological Products; D026121:Indole Alkaloids; D014748:Vinca Alkaloids; C057239:vobasine; D007050:Ibogaine; C011734:coronardine; D005980:Glutathione Reductase; D013880:Thioredoxin-Disulfide Reductase; C017836:catharanthine