Enhanced uptake of liposomes by bovine macrophages after opsonization with antibodies to Brucella abortus.
Phosphatidylcholine:cholesterol liposomes containing 4(5) carboxyfluorescein were modified by incorporation of Brucella abortus lipopolysaccharide. These vesicles were opsonized with bovine anti-B abortus or anti-B abortus plus complement. The fluorescent marker entrapped in antibody-opsonized liposomes was taken up by cultured bovine macrophages more effectively than was the same compound which had been loaded into nonopsonized liposomes. The addition of bovine complement to antibody-opsonized liposomes did not increase the extent of 4(5) carboxyfluorescein uptake over that caused by antibodies alone. The system, described in this report, could be used as a model for evaluation of the efficacy of selectively directing liposomes containing antibiotics to macrophages infected with intracellular B abortus, since macrophages possess Fc receptors capable of binding antibody-opsonized liposomes.
Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences and College of Veterinary Medicine, University of Florida, Gainesville, 32611, USA.
V
V
Quelch
K R
KR
Reed
G W
GW
Erdos
P L
PL
Nicoletti
E M
EM
Hoffmann
D000818:Animals; D000907:Antibodies, Bacterial / Q000502:physiology*; D002003:Brucella abortus / Q000276:immunology*; D002417:Cattle; D002478:Cells, Cultured; D002784:Cholesterol / Q000737:chemistry; D005260:Female; D008081:Liposomes / Q000378:metabolism*; D008264:Macrophages / Q000502:physiology*; D010713:Phosphatidylcholines / Q000737:chemistry
D000907:Antibodies, Bacterial; D008081:Liposomes; D010713:Phosphatidylcholines; D002784:Cholesterol