Effects of stimulation of glutamate receptors in medial septum on some immune responses in rats.
The immunomodulatory role of medial septum (MS) has been explored so far only in MS lesioned rats. But in MS lesioned rats, all the nerve cells and fibres of the lesioned area are damaged and the specific role of the neural circuits of MS on immunomodulation cannot be assessed from the lesion of MS. Considering the presence of a large number of glutamate receptors in MS, the specific role of glutamate receptors stimulation on some immune responses has been investigated in the present study. Hyperreactive behaviour, TC and DC of WBC, phagocytic activity of peripheral leukocytes, adhesibility and cytotoxicity of splenic mononuclear cells (MNC), delayed type of hypersensitivity (DTH) responses and the serum corticosterone (CORT) were measured after microinfusion of glutamate into MS of rats. To ascertain the specific role of those glutamate receptors, the parameters were also measured after microinfusion of glutamate receptor blocker 6, 7-dinitroquinoxaline-2, 3-dione (DNQX). The hyperreactive behaviour, TC and DC of WBC remained unaltered after stimulation or blocking of glutamate receptors. The phagocytic activity, adhesibility and cytotoxicity of splenic MNC, and DTH responses were increased after infusion of 0.25 and 0.5µM glutamate. But after infusion of higher dose of glutamate (1µM), the phagocytic activity and the adhesibility of splenic MNC were decreased and other parameters remained unaltered in that condition. After infusion of 4 and 8mM DNQX all the observed immunological parameters were decreased. The CORT concentration was decreased after infusion of 0.25 and 0.5µM of glutamate but it was increased after infusion of 1µM glutamate or 4 and 8mM DNQX. Results indicate that the medial septal glutamate receptors play an important role in the modulation of some immune responses.
Department of Physiology, University Colleges of Science and Technology, University of Calcutta, 92, APC Road, Kolkata 700009, India.
Goutam
G
Dutta
Ananda
A
Raj Goswami
Tusharkanti
T
Ghosh
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D003345:Corticosterone / Q000097:blood; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D018698:Glutamic Acid / Q000494:pharmacology; D007962:Leukocytes / Q000502:physiology; D008297:Male; D010587:Phagocytosis; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D017470:Receptors, Glutamate / Q000187:drug effects / Q000502:physiology*; D020665:Septum of Brain / Q000187:drug effects / Q000276:immunology*
D018690:Excitatory Amino Acid Agonists; D018691:Excitatory Amino Acid Antagonists; D011810:Quinoxalines; D017470:Receptors, Glutamate; D018698:Glutamic Acid; C056723:FG 9041; D003345:Corticosterone
0.25µM glutamate infused rats; 0.5µM glutamate infused rats; 1µM glutamate infused rats; 2mM DNQX infused rats; 4mM DNQX infused rats; 8mM DNQX infused rats; BSA; C; CORT; CV; DC; DNQX-6,7; DNQX1; DNQX2; DNQX3; DTH; EDTA; FACS; FITC; GLU1; GLU2; GLU3; Glutamate receptor; Immunomodulation; LAI; LDH; MNC; MS; Medial septum; Microinfusion; NKCC; PBS; PI; TC; bovine serum albumin; control; corticosterone; delayed type of hypersensitive; differential count; dinitroquinoxaline-2, 3-dione; ethylenediaminetetraacetic acid; fluorescein isothiocyanate; fluorescence activated cell sorter; lactate dehydrogenize; leukocyte adhesive inhibition index; mM; medial septum; micromolar; milimolar; mononuclear cell; natural killer cell cytotoxicity; phagocytic index; phosphate buffer saline; total count; vehicle infused control; µM