Parts per trillion detection of 7-aminonitrazepam by nano-enhanced ELISA.
It is challenging to detect 7-aminonitrazepam (7-ANZP) residue in animal tissues simply and sensitively by the enzyme-linked sorbent immunoassay (ELISA) method. This paper demonstrates that utilizing a bioconjugate of gold nanoparticles and enzyme-labeled antibody as a signal probe increases the sensitivity of a traditional ELISA for 7-ANZP by nearly 20 times. The sensitivity of this ELISA for 7-ANZP was 5.6 pg/mL in buffer, and the limit of detection (LOD) of 0.18 µg/kg for 7-ANZP in urine could be achieved after the urine samples were simply hydrolyzed and diluted by buffer. This simple and sensitive method has potential application for improving the sensitivity of ELISA methods against various small molecules.
State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. pcf@jiangnan.edu.cn.
Chifang
C
Peng
Xiaohui
X
Duan
Shanshan
S
Song
Feng
F
Xue
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006046:Gold / Q000737:chemistry; D018796:Immunoconjugates / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry*; D009567:Nitrazepam / Q000031:analogs & derivatives* / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D012680:Sensitivity and Specificity
D018796:Immunoconjugates; D007074:Immunoglobulin G; D011506:Proteins; C043577:7-aminonitrazepam; D006046:Gold; D009567:Nitrazepam