Comparative tissue distribution profiles of five major bio-active components in normal and blood deficiency rats after oral administration of Danggui Buxue Decoction by UPLC-TQ/MS.
Astragali Radix (AR) and Angelicae Sinensis Radix (ASR) were frequently combined and used in China as herbal pair called as Danggui Buxue Decoction (DBD) for treatment of blood deficiency syndrome, such as women's ailments. This study is to investigate the tissue distribution profiles of five major bio-active constituents (ferulic acid, caffeic acid, calycosin-7-O-β-glucoside, ononin and astragaloside IV) in DBD after oral administration of DBD in blood deficiency rats, and to compare the difference between normal and blood deficiency rats. The blood deficiency rats were induced by bleeding from orbit at the dosages of 5.0mLkg(-1) every day, and the experimental period was 12 days. At the finally day of experimental period, both normal and blood deficiency rats were orally administrated with DBD, and then the tissues samples were collected at different time points. Ferulic acid, caffeic acid, calycosin-7-O-β-glucoside, ononin and astragaloside IV in different tissues were detected simultaneously by UPLC-TQ/MS, and the histograms were drawn. The results showed that the overall trend was CLiver>CKidney>CHeart>CSpleen>CLung, CC-30min>CM-30min>CM-60min>CC-5min>CM-5min>CC-60min>CM-240min>CC-240min. The contents of the detected compounds in liver were more than that in other tissues no matter in normal or blood deficiency rats. Compared to normal rats, partial contents of the compounds in blood deficiency rats' tissues at different time points had significant difference (P<0.05). This study was the first report about tissue distribution investigation in blood deficiency animals which is conducted by bleeding. And the results demonstrated that the five DBD components in normal and blood deficiency rats had obvious differences in some organs and time points, suggesting that the blood flow and perfusion rate of the organ were altered in blood deficiency animals.
Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, 138 Xianlin Road, Nanjing 210023, China.
Xuqin
X
Shi
Yuping
Y
Tang
Huaxu
H
Zhu
Weixia
W
Li
Zhenhao
Z
Li
Wei
W
Li
Jin-ao
JA
Duan
D000284:Administration, Oral; D000740:Anemia / Q000097:blood* / Q000188:drug therapy; D000818:Animals; D002109:Caffeic Acids / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000097:blood / Q000493:pharmacokinetics; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D005260:Female; D005960:Glucosides / Q000097:blood / Q000493:pharmacokinetics; D007529:Isoflavones / Q000097:blood / Q000493:pharmacokinetics; D008297:Male; D013058:Mass Spectrometry; D008516:Medicine, Chinese Traditional; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012503:Saponins / Q000097:blood / Q000493:pharmacokinetics; D014018:Tissue Distribution; D014315:Triterpenes / Q000097:blood / Q000493:pharmacokinetics
D002109:Caffeic Acids; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D007529:Isoflavones; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; C526426:calycosin-7-O-beta-D-glucoside; C477279:danggui buxue decoction; C052064:astragaloside A; C004999:ferulic acid; C040048:caffeic acid
Bleeding; Blood deficiency; Blood loss anemia; Danggui Buxue Decoction; Tissue distribution; UPLC-TQ/MS