Generation of induced pluripotent stem cells with high efficiency from human umbilical cord blood mononuclear cells.
Human induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine. Generating iPSCs from immunologically immature newborn umbilical cord blood mononuclear cells (UCBMCs) is of great significance. Here we report generation of human iPSCs with great efficiency from UCBMCs using a dox-inducible lentiviral system carrying four Yamanaka factors. We generated these cells by optimizing the existing iPSC induction protocol. The UCBMC-derived iPSCs (UCB-iPSCs) have characteristics that are identical to pluripotent human embryonic stem cells (hESCs). This study highlights the use of UCBMCs to generate highly functional human iPSCs that could accelerate the development of cell-based regenerative therapy for patients suffering from various diseases.
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; College of Life Science, Northeast Agricultural University of China, Harbin 150030, China.
Juan
J
Wang
Qi
Q
Gu
Jie
J
Hao
Donghui
D
Bai
Lei
L
Liu
Xiaoyang
X
Zhao
Zhonghua
Z
Liu
Liu
L
Wang
Qi
Q
Zhou
D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D005312:Fetal Blood / Q000166:cytology* / Q000378:metabolism; D005855:Germ Layers / Q000166:cytology / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D007231:Infant, Newborn; D016086:Lentivirus / Q000235:genetics
D015415:Biomarkers
Dox-inducible lentiviral system; Human UCBMCs; Human iPSCs