In utero electroporation to study mouse brain development.
In utero electroporation is a rapid and powerful technique to study the development of many brain regions. This approach presents several advantages over other methods to study specific steps of brain development in vivo, from proliferation to synaptic integration. Here, we describe in detail the individual steps necessary to carry out the technique. We also highlight the variations that can be implemented to target different cerebral structures and to study specific steps of development.
Neurocentre Magendie, Physiopathologie de la Plasticité Neuronale, U862, Institut National de la Santé et de la Recherche Médicale, Université de Bordeaux, Bordeaux, France.
Emilie
E
Pacary
François
F
Guillemot
D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology* / Q000601:surgery; D004247:DNA / Q000378:metabolism; D018274:Electroporation / Q000379:methods*; D051379:Mice
D004247:DNA