Selection of suitable reference genes for mRNA quantification studies using common marmoset tissues.
The common marmoset (Callithrix jacchus) is increasingly being used as a non-human primate animal model in biomedical research. To perform accurate quantitative analysis of gene expression by quantitative reverse transcription polymerase chain reaction, reliable reference genes should be selected. In this study, we evaluated the expressions of 11 widely used reference genes: ACTB, ATP5F1, B2M, GAPDH, HPRT1, PGK1, PPIA, RN18S1, RPLP0, TBP and UBC in 12 tissues and five brain areas of healthy common marmosets. NormFinder and geNorm indicated that the most suitable reference genes for cross-sectional studies of the 17 tissues were RN18S1 and RPLP0. Conversely, ACTB and PPIA were the most suitable for analyzing brain samples; however, the expression of PGK1 fluctuated among brain areas. These results indicate that suitable reference genes differ between the tissues examined. This study provides fundamental information for gene expression studies of the common marmoset and highlights the importance of validating reference genes before quantification of target mRNAs.
Department of Veterinary Teaching Hospital, School of Veterinary Medicine, Kitasato University, Towada, Aomori, 034-8628, Japan.
Yoshinori
Y
Shimamoto
Hiroshi
H
Kitamura
Kimie
K
Niimi
Yasunaga
Y
Yoshikawa
Fumio
F
Hoshi
Mayumi
M
Ishizuka
Eiki
E
Takahashi
D000818:Animals; D002144:Callithrix / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D009928:Organ Specificity / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012015:Reference Standards; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012984:Software
D012333:RNA, Messenger