Goose fat, a promising nutrient for fish feeding, activates antioxidant enzymes in rainbow trout, Oncorhynchus mykiss.
The objective of this experiment was to test effects of different dietary lipids in rainbow trout feeding on the activity and expression of antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione S-transferase (GST). Four iso-nitrogenous and iso-lipidic casein-gelatin based experimental diets were formulated. The sources of dietary lipids were cod liver oil (CO, rich in polyunsaturated fatty acids), goose fat (GF, rich in saturated fatty acids and monounsaturated fatty acids), soybean oil (SO, rich in linoleic acid), and a blend of CO, GF and SO. Dietary treatments had no significant effect on growth performance and survival was not affected. SOD, GPx and GST enzymes had the maximum activity in GF diet. However qPCR data showed that SOD and GPx mRNA levels were minimum in GF group. Overall data showed that rainbow trout liver enzymes were activated upon GF diet probably activating the enzyme structure itself without stimulating gene expression.
Ondokuz Mayis University, Faculty of Agriculture, Department of Agricultural Biotechnology, 55139 Samsun, Turkey. Electronic address: lmercan@omu.edu.tr.
Levent
L
Mercan
Necdet
N
Sirkecioğlu
Ercüment
E
Aksakal
Mehtap
M
Bayır
Abdulkadir
A
Bayır
Mevlüt
M
Aras
Deniz
D
Ekinci
D000821:Animal Feed; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D004032:Diet; D004041:Dietary Fats / Q000032:analysis / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects*; D005227:Fatty Acids / Q000737:chemistry; D005395:Fish Oils / Q000494:pharmacology; D005777:Geese / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008055:Lipids / Q000737:chemistry; D017686:Oncorhynchus mykiss / Q000502:physiology*; D014176:Protein Biosynthesis / Q000187:drug effects; D060888:Real-Time Polymerase Chain Reaction; D013024:Soybean Oil / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D018076:DNA, Complementary; D004041:Dietary Fats; D005227:Fatty Acids; D005395:Fish Oils; D008055:Lipids; D013024:Soybean Oil; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase
Antioxidant; Fish oil; Glutathione peroxidase; Lipids; Rainbow trout; Superoxide dismutase