[Cloning and bioinformatics analysis of SmNAC1 from Salvia miltiorrhiza hairy root].
In order to study function of NAC transcription in development, hormone regulation and the stress response of Salvia miltiorrhiza, the NAC transcription was cloned and analyzed. By retrieving cDNA database of S. miltiorrhiza hairy root one NAC unigene was found, then a full length of cDNA was cloned by designing specific primers and PCR amplifying. Using ORF finder it was found that the cDNA containing a NAC-AB conserved domain in N-terminal, so the cDNA was a NAC transcription factor, named as SmNAC1 (kF006346). Bioinformatics analysis showed that SmNAC1 had an open reading frame (ORF) of 591 bp encoding 196 amino acids. The calculated protein had isoelectric point (pI) of 4.36 with molecular weight about 21.66 kDa. The transcription level of SmNAC1 after dealing with yeast extract (YE) and silver ion (Ag+) in S. miltiorrhiza hairy root was markedly stimulated up regulating. It was 1.4 fold compared with the control after induction 2 h, and maintained 2.0 fold on 4-12 h after induction. SmNAC1 may participate in regulation of stress response of YE + Ag+.
National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Ya-Jun
YJ
Wang
Chao
C
Jiang
Rong
R
Zhao
Le
L
Zhao
Ye
Y
Shen
Lu-Qi
LQ
Huang
D003001:Cloning, Molecular; D019295:Computational Biology; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000502:physiology; D018517:Plant Roots / Q000737:chemistry; D027561:Salvia miltiorrhiza / Q000737:chemistry* / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics* / Q000502:physiology
D010940:Plant Proteins; D015534:Trans-Activators