miR-202 expression concentration and its clinical significance in the serum of multiple myeloma patients.
OBJECTIVE
To explore microRNA-202 (miR-202) expression in serum of patients with multiple myeloma (MM), and investigate correlations between serum miR-202 expression and the development and prognosis of MM.


METHODS
RNA was extracted from serum by QIAGEN miRNeasy Mini kit. Reverse transcription was performed with specific stem-loop primers. SYBR Green I QF-PCR was applied to detect the relative expression of miR-202 in 40 MM patients and 30 healthy controls. The linearity, specificity and reproducibility were evaluated. In addition, correlations between the relative expression of serum miR-202 and the concentrations of lactic acid dehydrogenase (LDH), β2M, λ light chain and κ light chain were assessed.


RESULTS
The relative expression of miR-202 in MM patients 1.503 (0.161-9.831) was significantly higher than that in healthy controls 1.000 (0.105-3.046) (P < 0.01) and was significantly correlated with serum β2M and κ light chain concentrations (r = 0.366, P = 0.0305; r = 0.358, P = 0.0348).


CONCLUSIONS
The relative expression of serum miR-202 in MM patients was significantly higher than that in healthy controls, and therefore it may prove to be useful in the auxiliary diagnosis of MM.
Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong Jiangsu, China.
Jiajia
J
Yu
Department of Radiation Oncology, Affiliated Hospital of Nantong University, Nantong Jiangsu, China.
Xiaojun
X
Qiu
Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong Jiangsu, China.
Xianjuan
X
Shen
Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong Jiangsu, China.
Wei
W
Shi
Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong Jiangsu, China.
Xinhua
X
Wu
The First Hospital Affiliated to Soochow University, Suzhou Jiangsu, China.
Guohao
G
Gu
Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, People's Republic of China.
Baoli
B
Zhu
Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong Jiangsu, China Department of Radiation Oncology, Affiliated Hospital of Nantong University, Nantong Jiangsu, China Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong Jiangsu, China Public Health School of Nantong University, Nantong Jiangsu, China jsq814@hotmail.com zhubl@jscdc.cn.
Shaoqing
S
Ju
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics*; D012372:ROC Curve; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D001613:beta 2-Microglobulin / Q000097:blood
D014408:Biomarkers, Tumor; C569392:MIRN202 microRNA, human; D035683:MicroRNAs; D001613:beta 2-Microglobulin
SYBR Green I fluorescent quantitative PCR; microRNA-202; multiple myeloma