[Preparation and in vitro permeation of tetramethylpyrazine hydrochloride transdermal gel].
The purpose of this study was to prepare tetramethylpyrazine hydrochloride transdermal gel and to study its permeation ability in vitro. The skin permeation ability was evaluated by Valian-Chien permeation cells with isolated rat skin. The concentration of tetramethylpyrazine in samples was determined by HPLC. The optimal formulation was composed with 5% azone, 5% peppermint oil, 8% sodium carboxymethylcellulose and 8% tetramethylpyrazine hydrochloride. The accumulative permeation amount of the gel was (6 731.87 +/- 102.31) microg x cm(-2) in 12 h,and the permeation rate was (535.02 +/- 33.89) microg x cm(-2) x h(-1). The release profile in vitro was in line with zero-order formulation. Tetramethylpyrazine hydrochloride gel prepared in the study would be developed as a novel transdermal preparation.
Department of Pharmaceutics, Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China. tangzhan1986@126.com
Zhan
Z
Tang
Hong-Yan
HY
Xu
Qiao
Q
Wang
D000279:Administration, Cutaneous; D000818:Animals; D002626:Chemistry, Pharmaceutical; D005782:Gels; D066298:In Vitro Techniques; D008297:Male; D010539:Permeability; D011719:Pyrazines / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D005782:Gels; D011719:Pyrazines; C017953:tetramethylpyrazine