Protein induced fluorescence enhancement (PIFE) for probing protein-nucleic acid interactions.
Single molecule studies of protein-nucleic acid interactions shed light on molecular mechanisms and kinetics involved in protein binding, translocation, and unwinding of DNA and RNA substrates. In this review, we provide an overview of a single molecule fluorescence method, termed "protein induced fluorescence enhancement" (PIFE). Unlike FRET where two dyes are required, PIFE employs a single dye attached to DNA or RNA to which an unlabeled protein is applied. We discuss both ensemble and single molecule studies in which PIFE was utilized.
Bioengineering Department, University of Illinois at Urbana-Champaign, USA. smyong@illinois.edu.
Helen
H
Hwang
Sua
S
Myong
D000818:Animals; D004867:Equipment Design; D006801:Humans; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D009696:Nucleic Acids / Q000032:analysis / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods
D009696:Nucleic Acids; D011506:Proteins