Photoluminescent C-dots@RGO for sensitive detection of hydrogen peroxide and glucose.
We have demonstrated sensitive detections of hydrogen peroxide (H2O2) and glucose using reduced graphene oxide decorated with carbon dots (C-dots@RGO). The C-dots@RGO prepared from catechin (reducing agent and carbon source) and graphene oxide via hydrothermal routes possesses excitation-wavelength-dependence photoluminescence (PL) characteristics, with maximum excitation and emission wavelengths of 365 and 440 nm, respectively. The C-dots@RGO is stable in solution containing NaCl up to 350 mM, but is quenched by reactive oxygen species (ROS). ROS reacts with H2O2 and thus its PL quenching toward the C-dots@RGO is minimized. When using C-dots@RGO and glucose oxidase (GOx), the PL assay allows detection of glucose in the presence of 10 µM of bovine serum albumin, with linearity over a concentration range from 1 to 60 µM (r=0.99) and a limit of detection (at a signal-to-noise ratio of 3) of 140 nM. The practicality of this assay has been validated by determining the concentrations of glucose in serum and saliva samples, with results of 5.1 ± 0.6mM (n=3) and 117.9 ± 8.1 μM (n=3), respectively. Our simple and sensitive assay opens a new avenue of developing assays for various analytes using C-dots@RGO in conjunction with different enzymes.
Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei, Taiwan.
Ting-Yin
TY
Yeh
Chen-I
CI
Wang
Huan-Tsung
HT
Chang
D000328:Adult; D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D002392:Catechin / Q000737:chemistry; D002417:Cattle; D004566:Electrodes; D005949:Glucose Oxidase / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood*; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D010087:Oxides; D055668:Photochemical Processes; D012463:Saliva / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D059629:Signal-To-Noise Ratio
D001786:Blood Glucose; D010087:Oxides; D012710:Serum Albumin, Bovine; D006108:Graphite; D002392:Catechin; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase
C-dots; Glucose; Hydrogen peroxide; Photoluminescent; Reduced graphene oxide