Ox-LDL increases OX40L in endothelial cells through a LOX-1-dependent mechanism.
Oxidative low-density lipoprotein (Ox-LDL) is a key risk factor for the development of atherosclerosis, and it can stimulate the expression of a variety of inflammatory signals. As a new and highly sensitive inflammation index, OX40L may be a key to understanding the mechanisms that regulate interactions between cells within the vessel wall and inflammatory mediators during the development of atherosclerosis. To investigate whether Ox-LDL regulates OX40L expression through an oxidized LDL-1 receptor (LOX-1)-mediated mechanism, we investigated the effect of different concentrations of Ox-LDL (50, 100, 150 µg/mL) on endothelial cell proliferation and apoptosis. Stimulation with Ox-LDL increased OX40L protein 1.44-fold and mRNA 4.0-fold in endothelial cells, and these effects were inhibited by blocking LOX-1. These results indicate that LOX-1 plays an important role in the chronic inflammatory process in blood vessel walls. Inhibiting LOX-1 may reduce blood vessel inflammation and provide a therapeutic option to limit atherosclerosis progression.
Department of Cardiology, The First Affiliated Hospital, Chongqing Medical University, Chongqing, China.
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Dong
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Xiang
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Zhang
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Qin
D017209:Apoptosis / Q000187:drug effects*; D050197:Atherosclerosis / Q000209:etiology / Q000517:prevention & control; D002453:Cell Cycle; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D008077:Lipoproteins, LDL / Q000378:metabolism / Q000494:pharmacology* / Q000502:physiology; D053278:OX40 Ligand / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D051127:Scavenger Receptors, Class E / Q000378:metabolism*; D015398:Signal Transduction; D014657:Vasculitis / Q000503:physiopathology / Q000517:prevention & control
D008077:Lipoproteins, LDL; C495137:OLR1 protein, human; D053278:OX40 Ligand; D051127:Scavenger Receptors, Class E; C089251:TNFSF4 protein, human; C099252:oxidized low density lipoprotein