Biodiesel production in a magnetically-stabilized, fluidized bed reactor with an immobilized lipase in magnetic chitosan microspheres.
Biodiesel production by immobilized Rhizopus oryzae lipase in magnetic chitosan microspheres (MCMs) was carried out using soybean oil and methanol in a magnetically-stabilized, fluidized bed reactor (MSFBR). The maximum content of methyl ester in the reaction mixture reached 91.3 (w/v) at a fluid flow rate of 25 ml/min and a magnetic field intensity of 150 Oe. In addition, the MCMs-immobilized lipase in the reactor showed excellent reusability, retaining 82 % productivity even after six batches, which was much better than that in a conventional fluidized bed reactor. These results suggested that a MSFRB using MCMs-immobilized lipase is a promising method for biodiesel production.
State Key Laboratory of Engines, School of Environmental Science and Technology, Tianjin University, Tianjin, 300072, China, guixiongzhou@163.com.
Gui-Xiong
GX
Zhou
Guan-Yi
GY
Chen
Bei-Bei
BB
Yan
D056804:Biofuels; D019149:Bioreactors; D048271:Chitosan / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D004951:Esterification; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D060328:Magnetic Phenomena; D000432:Methanol / Q000378:metabolism; D008863:Microspheres; D013024:Soybean Oil / Q000378:metabolism
D056804:Biofuels; D004800:Enzymes, Immobilized; D013024:Soybean Oil; D048271:Chitosan; D008049:Lipase; D000432:Methanol