Modulatory effect of Chandraprabha Vati on antimicrobial peptides and inflammatory markers in kidneys of mice with urinary tract infection.
BACKGROUND
Chandraprabha Vati (CV) is an Indian polyherbal Siddha drug, traditionally used as an anti-inflammatory agent for arthritis and urinary ailments. This study explores its effect on mice with urinary tract infection.


METHODS
The in-organic constituents of CV were determined by atomic absorption spectroscopy and phytochemical analysis was carried out. The supplementing dose of CV to infected experimental mice was determined by in vitro antimicrobial assay. Transurethrally infected animals were supplemented with CV extract for 20 days after confirmation of UTI. The animals were euthanized as per the guidelines and the tissues were harvested from the control and infected mice for histopathological examination the antimicrobial peptide Tamm-Horsfall protein (THP) and inflammatory markers (tumor necrosis factor-α and nuclear factor kappa-light-chain-enhancer of activated B cells) to ascertain the modulatory effects of CV. Indicators for oxidative stress and protein levels were also quantified to validate the efficacy of CV.


RESULTS
Terpenoids and flavanoids were majorly found to constitute CV along with zinc and iron as in-organic content. Histological and immunohistochemical studies confirmed the pronounced infection in the kidney of the uropathogenic Escherichia coli-infected animals. Supplementation of CV significantly restored the increased levels of the antimicrobial proteins, THP, and inflammatory markers.


CONCLUSIONS
This study explored the efficacy of the aqueous extract of CV as an alternative medication for the synthetic analogues administered for UTI. This study also provides information on the possible role of THP as an antimicrobial protein in the kidney in preventing infection due to uropathogenic E coli.
Renal Research Laboratory, Centre for Bio Medical Research, School of Biosciences and Technology, VIT University, Vellore, Tamilnadu, India. pragasam.v@vit.ac.in.
Suneeva S
SS
Christa
Adaveni
A
Swetha
Evageline
E
Christina
Rajesh
R
Ganesh
Pragasam
P
Viswanathan
D000818:Animals; D028526:Coriandrum / Q000737:chemistry; D030024:Curcuma / Q000737:chemistry; D029786:Cyperus / Q000737:chemistry; D004195:Disease Models, Animal; D005260:Female; D020939:Zingiber officinale / Q000737:chemistry; D007668:Kidney / Q000382:microbiology / Q000473:pathology*; D008515:Medicine, Ayurvedic; D051379:Mice; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism; D029842:Phyllanthus emblica / Q000737:chemistry; D031701:Piper / Q000737:chemistry; D028321:Plant Preparations / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000378:metabolism; D001743:Urinary Bladder / Q000382:microbiology / Q000473:pathology*; D014552:Urinary Tract Infections / Q000188:drug therapy*; D058949:Uromodulin / Q000187:drug effects / Q000378:metabolism; D056689:Uropathogenic Escherichia coli / Q000187:drug effects
D016328:NF-kappa B; D028321:Plant Preparations; D014409:Tumor Necrosis Factor-alpha; D058949:Uromodulin