Altitudinal variation of antioxidant components and capability in Indocalamus latifolius (Keng) McClure leaf.
Indocalamus latifolius (Keng) McClure leaf is a popular food material in East Asia due to its antioxidant and anticorrosive activities. To utilize it more effectively, we investigated the discrepancy of antioxidant activities and active compound content in Indocalamus latifolius leaf along with the altitude change. Total flavonoids, phenolics, titerpenoids and eight characteristic active constituents, i.e, orientin, isoorientin, vitexin, homovitexin, p-coumaric acid, chlorogenic acid, caffeic acid, and ferulic acid, were determined by UV-spectrophotometer and synchronous RP-HPLC, respectively. Antioxidant activity was measured using DPPH and FRAP methods. Our data showed that the content of TP and TF, DPPH radical scavenging ability and ferric reduction power of Indocalamus latifolius leaf changed as altitude altered, with the trends of decreasing gradually when lower than 700 m and then increasing to 1,000 m. Chlorogenic acid and orientin were the main characteristic compounds in Indocalamus latifolius leaf and were also affected by altitude. Our result indicated that higher altitude with an adverse environment is conducive to secondary metabolite accumulation for Indocalamus latifolius. It would provide a theoretical basis to regulate the leaf collection conditions in the industrial use of Indocalamus latifolius leaf.
The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Zhejiang Agriculture and Forestry University.
Qinxue
Q
Ni
Zhiqiang
Z
Wang
Guangzhi
G
Xu
Qianxin
Q
Gao
Dongdong
D
Yang
Fumiki
F
Morimatsu
Youzuo
Y
Zhang
D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D047310:Apigenin / Q000032:analysis / Q000494:pharmacology; D001208:Asia; D001713:Biphenyl Compounds / Q000378:metabolism; D002109:Caffeic Acids / Q000032:analysis / Q000494:pharmacology; D002726:Chlorogenic Acid / Q000032:analysis / Q000494:pharmacology*; D003373:Coumaric Acids / Q000032:analysis / Q000494:pharmacology; D004032:Diet; D017753:Ecosystem; D005290:Ferric Compounds / Q000378:metabolism; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology*; D005960:Glucosides / Q000032:analysis / Q000494:pharmacology*; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D006109:Poaceae / Q000737:chemistry*; D059808:Polyphenols / Q000032:analysis / Q000494:pharmacology; D064210:Secondary Metabolism; D014315:Triterpenes / Q000032:analysis / Q000494:pharmacology
D000975:Antioxidants; D001713:Biphenyl Compounds; D002109:Caffeic Acids; D003373:Coumaric Acids; D005290:Ferric Compounds; D005419:Flavonoids; D005960:Glucosides; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D014315:Triterpenes; D002726:Chlorogenic Acid; D047310:Apigenin; C032731:vitexin; C004999:ferulic acid; C004931:1,1-diphenyl-2-picrylhydrazyl; C065886:orientin; C040048:caffeic acid