The time-dependent manner of sinusoidal electromagnetic fields on rat bone marrow mesenchymal stem cells proliferation, differentiation, and mineralization.
Electromagnetic fields (EMFs) are used clinically to promote fracture healing and slow down osteoporosis without knowledge of optimal parameters and underlying principles. In the present study, we investigate the effects of irritation for different durations with 15 Hz 1 mT sinusoidal EMFs (SEMFs) on rat bone marrow mesenchymal stem cells (BMSCs) proliferation, differentiation, and mineralization potentials. Our results show that SEMFs irritation promote rat BMSCs proliferation in a time-dependent manner, and the expression of osteogenic gen [Cbfa 1/RUNX2, bone sialoprotein (BSP), osteopontin (OPN)], alkaline phosphatase activity, and calcium deposition were enhanced after SEMFs treatment depending on the time duration of treatment. To determine the role of MEK/ERK signaling pathway, U0126, a MEK/ERK inhibitor was used. It can suppress rat BMSCs' proliferation with or without SEMF exposure, and partly attenuate the expression of osteogenesis related proteins (RUNX2, BSP, OPN) which were improved by SEMF. This finding suggests that the effects of SEMF on rat BMSCs' proliferation differentiation and mineralization are time duration dependent and MEK/ERK signaling pathway plays important role.
Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430000, Hubei, China, langzhiwu126@126.com.
Ming-Yu
MY
Song
Ji-Zhe
JZ
Yu
Dong-Ming
DM
Zhao
Sheng
S
Wei
Yang
Y
Liu
Yue-Ming
YM
Hu
Wen-Chun
WC
Zhao
Yong
Y
Yang
Hua
H
Wu
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D002070:Butadienes / Q000494:pharmacology; D002113:Calcification, Physiologic / Q000528:radiation effects*; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D004574:Electromagnetic Fields; D048049:Extracellular Signal-Regulated MAP Kinases; D005260:Female; D005786:Gene Expression Regulation; D058574:Integrin-Binding Sialoprotein / Q000235:genetics / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D009570:Nitriles / Q000494:pharmacology; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013997:Time Factors
D002070:Butadienes; D050659:Core Binding Factor Alpha 1 Subunit; D058574:Integrin-Binding Sialoprotein; D009570:Nitriles; D047428:Protein Kinase Inhibitors; C493717:Runx2 protein, rat; C506897:Spp1 protein, rat; C113580:U 0126; D053495:Osteopontin; D048049:Extracellular Signal-Regulated MAP Kinases; D000469:Alkaline Phosphatase; D002118:Calcium