Microarray detection and qPCR screening of potential biomarkers of Folsomia candida (Collembola: Isotomidae) exposed to Bt proteins (Cry1Ab and Cry1Ac).
The impact of Bt proteins on non-target arthropods is less understood than their effects on target organisms where the mechanism of toxic action is known. Here, we report the effects of two Bt proteins, Cry1Ab and Cry1Ac, on gene expression in the non-target collembolan, Folsomia candida. A customized microarray was used to study gene expression in F. candida specimens that were exposed to Cry1Ab and Cry1Ac. All selected transcripts were subsequently confirmed by qPCR. Eleven transcripts were finally verified, and three of them were annotated. The responses of all eleven transcripts were tested in specimens for both Cry1Ab and Cry1Ac at a series of concentrations. These transcripts were separated into two and three groups for Cry1Ab and Cry1Ac, respectively, depend on their expression levels. However, those eleven transcripts did not respond to the Bt proteins in Bt-rice residues.
State Key Laboratory of Integrated Management of Pest and Rodents, Institute of Zoology, Chinese Academy of Sciences, 1 Beichen West Road, Chaoyang District, Beijing 100101, China. Electronic address: yuanyy@ioz.ac.cn.
Yiyang
Y
Yuan
Paul Henning
PH
Krogh
Xue
X
Bai
Dick
D
Roelofs
Fajun
F
Chen
Keyan
K
Zhu-Salzman
Yuyong
Y
Liang
Yucheng
Y
Sun
Feng
F
Ge
D000818:Animals; D001181:Arthropods / Q000235:genetics / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000633:toxicity*; D015415:Biomarkers / Q000378:metabolism; D004731:Endotoxins / Q000633:toxicity*; D006460:Hemolysin Proteins / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D010572:Pest Control, Biological; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D040081:Protein Array Analysis
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D015415:Biomarkers; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis
Bt proteins; Folsomia candida; Microarray; Non-target organism