Cerebroside C increases tolerance to chilling injury and alters lipid composition in wheat roots.
Chilling tolerance was increased in seed germination and root growth of wheat seedlings grown in media containing 20 µg/mL cerebroside C (CC), isolated from the endophytic Phyllosticta sp. TG78. Seeds treated with 20 µg/mL CC at 4 °C expressed the higher germination rate (77.78%), potential (23.46%), index (3.44) and the shorter germination time (6.19 d); root growth was also significantly improved by 13.76% in length, 13.44% in fresh weight and 6.88% in dry mass compared to controls. During the cultivation process at 4 °C for three days and the followed 24 h at 25 °C, lipid peroxidation, expressed by malondialdehyde (MDA) content and relative membrane permeability (RMP) was significantly reduced in CC-treated roots; activities of lipoxygenase (LOX), phospholipid C (PLC) and phospholipid D (PLD) were inhibited by 13.62-62.26%, 13.54-63.93% and 13.90-61.17%, respectively; unsaturation degree of fatty acids was enhanced through detecting the contents of CC-induced linoleic acid, linolenic acid, palmitic acid and stearic acid using GC-MS; capacities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were individually increased by 7.69-46.06%, 3.37-37.96%, and -7.00-178.07%. These results suggest that increased chilling tolerance may be due, in part, to the reduction of lipid peroxidation and alternation of lipid composition of roots in the presence of CC.
College of Plant Protection, Jiangsu Key Laboratory of Pesticide Science, Nanjing Agricultural University, Nanjing, P. R. China ; Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, P. R. China ; School of Pharmacy, Jilin University, Changchun, P. R. China.
Hong-Xia
HX
Li
Yu
Y
Xiao
Ling-Ling
LL
Cao
Xu
X
Yan
Cong
C
Li
Hai-Yan
HY
Shi
Jian-Wen
JW
Wang
Yong-Hao
YH
Ye
D000222:Adaptation, Physiological / Q000187:drug effects*; D018533:Biomass; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D002463:Cell Membrane Permeability; D002554:Cerebrosides / Q000302:isolation & purification / Q000494:pharmacology*; D003080:Cold Temperature; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D018525:Germination / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D015227:Lipid Peroxidation; D008084:Lipoxygenase / Q000235:genetics / Q000378:metabolism; D008315:Malondialdehyde; D010743:Phospholipids / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D004718:Saccharomycetales / Q000737:chemistry; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014908:Triticum / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
D002554:Cerebrosides; D005231:Fatty Acids, Unsaturated; D010743:Phospholipids; D010940:Plant Proteins; C116025:cerebroside C; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D008084:Lipoxygenase; D013482:Superoxide Dismutase