Structure and digestibility of endosperm water-soluble α-glucans from different sugary maize mutants.
The structure and digestibility of endosperm water-soluble α-glucans from different sugary-1 maize mutants (Zhongtian 8#, Zhongtian 2# and Pintian 8#) were investigated. The yield of pure glucan was in the range of 25.91-34.38%. The α-glucan belonged to a typical native nano-scale particle and the average particle size was in the following order: Zhongtian 8# >Pintian 8# >Zhongtian 2#. The weight-average molar mass of glucans ranged from 1.69 to 2.08×10(7)g/mol. The branch densities and α-1,6 linkages of Zhongtian 8#, Zhongtian 2# and Pintian 8#, were 8.60%, 8.77% and 9.51%, 7.71%, 6.58% and 6.81%, respectively. The resistant starch (10.06%) of Pintian 8# was lower than other two cultivars. The study showed that water-soluble glucan exhibited α-1,4-linked backbone with α-1,6 branch sites and digestibility was influenced by granule size, ratio of α-1,4 to α-1,6 linkages, molecular fine structure in this set of sugary maize mutants.
State Key Laboratory of Food Science & Technology, Ministry of Education Key Laboratory of Carbohydrate Chemistry & Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, PR China. Electronic address: miaoming@jiangnan.edu.cn.
Ming
M
Miao
Rong
R
Li
Bo
B
Jiang
Steve W
SW
Cui
Keyu
K
Lu
Tao
T
Zhang
D002240:Carbohydrate Sequence; D004063:Digestion; D056625:Endosperm / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005936:Glucans / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D015394:Molecular Structure; D008970:Molecular Weight; D009154:Mutation; D012995:Solubility; D003313:Zea mays / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*
D005936:Glucans
Digestibility; Fine structure; Nanoparticle; Sugary maize; Water-soluble polysaccharide