Rosmarinus officinalis L. leaf extract improves memory impairment and affects acetylcholinesterase and butyrylcholinesterase activities in rat brain.
Rosmarinus officinalis L. leaf as part of a diet and medication can be a valuable proposal for the prevention and treatment of dementia. The aim of the study was to assess the effects of subchronic (28-fold) administration of a plant extract (RE) (200 mg/kg, p.o.) on behavioral and cognitive responses of rats linked with acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activity and their mRNA expression level in the hippocampus and frontal cortex. The passive avoidance test results showed that RE improved long-term memory in scopolamine-induced rats. The extract inhibited the AChE activity and showed a stimulatory effect on BuChE in both parts of rat brain. Moreover, RE produced a lower mRNA BuChE expression in the cortex and simultaneously an increase in the hippocampus. The study suggests that RE led to improved long-term memory in rats, which can be partially explained by its inhibition of AChE activity in rat brain.
Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznan, Poland; Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, Sw. Marii Magdaleny 14, 61-861 Poznan, Poland. Electronic address: mozarow@ump.edu.pl.
Marcin
M
Ozarowski
Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznan, Poland; Department of Pharmacology, Poznan University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland. Electronic address: przemmik@ump.edu.pl.
Przemyslaw L
PL
Mikolajczak
Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznan, Poland; Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, Poznan University of Medical Sciences, Swiecickiego 6, 61-781 Poznan, Poland. Electronic address: aniabogacz23@o2.pl.
Anna
A
Bogacz
Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznan, Poland. Electronic address: agnieszka.gryszczynska@iwnirz.pl.
Agnieszka
A
Gryszczynska
Department of Toxicology, Poznan University of Medical Sciences, Dojazd 30, 60-631 Poznan, Poland. Electronic address: kujawska@ump.edu.pl.
Malgorzata
M
Kujawska
Department of Toxicology, Poznan University of Medical Sciences, Dojazd 30, 60-631 Poznan, Poland. Electronic address: liebert@ump.edu.pl.
Jadwiga
J
Jodynis-Liebert
Department of Pathogen Genetics and Plant Resistance, Metabolomics Team, Institute of Plant Genetics of the Polish Academy of Science, Strzeszynska 34, 60-479 Poznan, Poland. Electronic address: akar@igr.poznan.pl.
Anna
A
Piasecka
Department of Pharmacology, Poznan University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland. Electronic address: hanna.napieczynska@gmail.com.
Hanna
H
Napieczynska
Department of Pharmacology, Poznan University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland. Electronic address: mszulc@ump.edu.pl.
Michał
M
Szulc
Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznan, Poland. Electronic address: kujawskiradoslaw@gmail.com.
Radoslaw
R
Kujawski
Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznan, Poland; Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, Poznan University of Medical Sciences, Swiecickiego 6, 61-781 Poznan, Poland. Electronic address: joanna@wieczorek.net.pl.
Joanna
J
Bartkowiak-Wieczorek
Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznan, Poland. Electronic address: joanna.cichocka@iwnirz.pl.
Joanna
J
Cichocka
Department of Pharmacology, Poznan University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland. Electronic address: tbobkiew@ump.edu.pl.
Teresa
T
Bobkiewicz-Kozlowska
Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznan, Poland; Department of General Pharmacology and Pharmacoeconomics, Pomeranian Medical University, Zolnierska 48, 70-204 Szczecin, Poland. Electronic address: bczerny@wp.pl.
Boguslaw
B
Czerny
Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznan, Poland; Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, Poznan University of Medical Sciences, Swiecickiego 6, 61-781 Poznan, Poland. Electronic address: pmm@post.pl.
Przemyslaw M
PM
Mrozikiewicz
D000110:Acetylcholinesterase / Q000235:genetics / Q000378:metabolism*; D000544:Alzheimer Disease / Q000188:drug therapy / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002091:Butyrylcholinesterase / Q000235:genetics / Q000378:metabolism*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D003071:Cognition / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008568:Memory / Q000187:drug effects*; D008569:Memory Disorders / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D018515:Plant Leaves; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D027542:Rosmarinus; D012601:Scopolamine
D002800:Cholinesterase Inhibitors; D010936:Plant Extracts; D012333:RNA, Messenger; D012601:Scopolamine; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase
Acetylcholinesterase; Butyrylcholinesterase; Carnosic acid (PubChem CID: 65126); Carnosol (PubChem CID: 2579); Huperzine A (PubChem CID: 1253); Memory; Rat; Rosemary; Rosmarinic acid (PubChem CID: 5281792); Scopolamine hydrobromide (CID: 5184)