Identification of an anticancer compound against HT-29 cells from Phellinus linteus grown on germinated brown rice.
OBJECTIVE
To isolate and identify the anticancer compound against proliferation of human colon cancer cells from ethyl acetate (EtOAc) extract of Phellinus linteus grown on germinated brown rice (PB).


METHODS
EtOAc extract of PB was partitioned with n-hexane, EtOAc, and water-saturated n-butanol. Anticancer compound of n-hexane layer was isolated and identified by HPLC and NMR, respectively. Cytotoxicity against HT-29 cells was tested by SRB assay.


RESULTS
The n-hexane layer obtained after solvent fractionation of PB EtOAc extracts showed a potent anticancer activity against the HT-29 cell line. Atractylenolide I, a eudesmane-type sesquiterpene lactone, a major anticancer substance of PB, was isolated from the n-hexane layer by silica gel column chromatography and preparative-HPLC. This structure was elucidated by one- and two-dimensional NMR spectroscopic data. Atractylenolide I has not been reported in mushrooms or rice as of yet. The isolated compound dose-dependently inhibited the growth of HT-29 human colon cancer cells.


CONCLUSIONS
Atractylenolide I might contribute to the anticancer effect of PB.
Department of Animal Science, College of Agriculture & Life Science, Chonnam National University, Korea.
Tae-Il
TI
Jeon
Chang-Hwa
CH
Jung
Jeong-Yong
JY
Cho
Dong Ki
DK
Park
Jae-Hak
JH
Moon
D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D001487:Basidiomycota / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D005591:Chemical Fractionation; D045424:Complex Mixtures / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D019073:HT29 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D007783:Lactones / Q000494:pharmacology / Q000633:toxicity; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D012275:Oryza / Q000382:microbiology; D012717:Sesquiterpenes / Q000494:pharmacology / Q000633:toxicity; D012997:Solvents
D000970:Antineoplastic Agents; D045424:Complex Mixtures; D007783:Lactones; D012717:Sesquiterpenes; D012997:Solvents; C424804:atractylenolide I
Atractylenolide I; Germinated brown rice; Human colon cancer cells; NMR; Phellinus linteus