Photoelectrochemical detection of glutathione by IrO2-hemin-TiO2 nanowire arrays.
We have developed sensitive detection of glutathione using the IrO2-hemin-TiO2 nanowire arrays. Single-crystalline TiO2 nanowires are synthesized by a hydrothermal reaction, followed by surface functionalization of ~3 nm thick hemin and ~1-2 nm diameter IrO2 nanoparticles. The IrO2-hemin-TiO2 nanowire arrays offer much enhanced photocurrent with ∼100% increase compared to the pristine TiO2 nanowires and allow for label-free, real-time, sensitive photoelectrochemical detection of glutathione. The sensitivity achieved is ~10 nM in buffer, comparable to or better than most of the existing glutathione detection methods. Furthermore, cell extracts containing glutathione are robustly detected, with ~8000 cells/mL for HeLa cells and ~5000 cells/mL for human embryonic kidney 293T cells. This nanowire PEC sensor assay exhibits excellent selectivity and stability, suggesting a potential detection platform for analyzing the glutathione level in biosamples.
Laboratory of Advanced Materials, Department of Chemistry, Fudan University , Shanghai, 200433, People's Republic of China.
Jing
J
Tang
Biao
B
Kong
Yongcheng
Y
Wang
Ming
M
Xu
Yanli
Y
Wang
Hao
H
Wu
Gengfeng
G
Zheng
D003460:Crystallization; D055664:Electrochemical Techniques; D005978:Glutathione / Q000737:chemistry / Q000302:isolation & purification*; D006427:Hemin / Q000737:chemistry; D006801:Humans; D007495:Iridium / Q000737:chemistry; D008027:Light; D053758:Nanoparticles / Q000737:chemistry*; D053770:Nanowires / Q000737:chemistry*; D010316:Particle Size; D014025:Titanium / Q000737:chemistry
C044458:iridium oxide; C009495:titanium dioxide; D007495:Iridium; D006427:Hemin; D014025:Titanium; D005978:Glutathione