[HPLC method to determine DEHP released into blood from a disposable extracorporeal circulation tube].
We used blood as leaching medium, simulating clinical operation under maximum condition, to develop Liquid-phase extraction- High Performance Liquid Chromatography (HPLC) method for determination of plasticizer Di-(2-ethylhexyl)phthalate (DEHP) released from Disposable Extracorporeal Circulation Tube in order to lay the foundation of risk analysis of this product. The characteristic wavelength of DEHP in methanol was detected. Acetonitrile was added to the leaching blood in proportion and extracted DEHP from blood. The methodology for HPLC to quantify DEHP was established and the DEHP amount released from this disposable extracorporeal circulation tube was measured. The experiments showed good results as follows. The characteristic wavelength of DEHP was 272nm. The concentration of DEHP (5-250 microg/mL) kept good linear relationship with peak area (r=0.9999). Method sensitivity was 1 microg/mL. Precisions showed RSD<5%. The adding standard extraction Recovery Rates of 25, 100 and 250 microg DEHP standard were 61.91 +/- 3.32)%, (69.38 +/- 0.55)% and (68.47 +/- 1.15)%. The DEHP maximum amounts released from 3 sets of this disposable extracorporeal circulation tube were 204.14, 106.30 and 165.34 mg/set. Our Liquid-phase Extraction-HPLC method showed high accuracy and precision, and relatively stable recovery rate. Its operation was also convenient.
Biomedical Engineering Research Center, Institute of Blood Transfusion of Chinese Academy of Medical Sciences, Chengdu 610052, China.
Xi
X
Liu
Jingjing
J
Yu
Shen
S
Li
Hong
H
Wang
Jiaxin
J
Liu
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003198:Computer Simulation; D004051:Diethylhexyl Phthalate / Q000032:analysis* / Q000097:blood*; D005112:Extracorporeal Circulation / Q000009:adverse effects / Q000295:instrumentation*; D006801:Humans; D010968:Plasticizers / Q000032:analysis
D010968:Plasticizers; D004051:Diethylhexyl Phthalate