Effect of low-level laser irradiation on osteoblast-like cells cultured on porous hydroxyapatite scaffolds.
OBJECTIVE
To determine the effect of laser irradiation at a low dose on human osteoblastlike cells.


METHODS
32 porous hydroxyapatite scaffolds currently used for bone tissue engineering were seeded with MG63 cells and irradiated or not with a GaAlAs diode laser (wavelength 915 nm, dose 2 J/cm2) using different power density and exposure duration.


RESULTS
After 72-h incubation, cells showed well spread morphology and good adhesion on both laser-treated and untreated scaffolds. Laser irradiation did not interfere in cell viability and proliferation as compared with the non-irradiated controls.


CONCLUSIONS
This study suggests that there is no effect of 915 nm laser irradiation at a dose of 2 J/cm2 on the proliferation rate of MG63 cells. Future investigations are needed to compare different dose and wavelength regimens in order to determine the optimal set of laser parameters for maximum cell yield and safe clinical application.
Reparto di Ortognatodonzia, Dipartimento di Scienze Biomediche e Neuromotorie (DIBINEM), Università di Bologna, Bologna, Italy. E-mail: serena.incerti2@unibo.it.
Serena
S
Incerti Parenti
Silvia
S
Panseri
Antonio
A
Gracco
Monica
M
Sandri
Anna
A
Tampieri
Giulio
G
Alessandri Bonetti
D001672:Biocompatible Materials; D032701:Biomimetics; D018786:Bone Substitutes; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000528:radiation effects*; D017886:Durapatite; D006801:Humans; D007834:Lasers; D010006:Osteoblasts / Q000528:radiation effects* / Q000648:ultrastructure; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D018786:Bone Substitutes; D017886:Durapatite