Magmas overexpression inhibits staurosporine induced apoptosis in rat pituitary adenoma cell lines.
Magmas is a nuclear gene that encodes for the mitochondrial import inner membrane translocase subunit Tim16. Magmas is overexpressed in the majority of human pituitary adenomas and in a mouse ACTH-secreting pituitary adenoma cell line. Here we report that Magmas is highly expressed in two out of four rat pituitary adenoma cell lines and its expression levels inversely correlate to the extent of cellular response to staurosporine in terms of apoptosis activation and cell viability. Magmas over-expression in rat GH/PRL-secreting pituitary adenoma GH4C1 cells leads to an increase in cell viability and to a reduction in staurosporine-induced apoptosis and DNA fragmentation, in parallel with the increase in Magmas protein expression. These results indicate that Magmas plays a pivotal role in response to pro-apoptotic stimuli and confirm and extend the finding that Magmas protects pituitary cells from staurosporine-induced apoptosis, suggesting its possible involvement in pituitary adenoma development.
Section of Endocrinology, Department of Medical Sciences, University of Ferrara, Ferrara, Italy.
Federico
F
Tagliati
Teresa
T
Gagliano
Erica
E
Gentilin
Mariella
M
Minoia
Daniela
D
Molè
Ettore C
EC
Delgi Uberti
Maria Chiara
MC
Zatelli
D000236:Adenoma / Q000235:genetics*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics*; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects; D045304:Cytochromes c / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D015870:Gene Expression; D026901:Membrane Transport Proteins / Q000235:genetics*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics*; D010911:Pituitary Neoplasms / Q000235:genetics*; D051381:Rats; D019311:Staurosporine / Q000494:pharmacology*
C000588811:Magmas-ps1 protein, rat; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D045304:Cytochromes c; D053148:Caspase 3; D053179:Caspase 7; D019311:Staurosporine