Revised structures for the predominant O-polysaccharides expressed by Burkholderia pseudomallei and Burkholderia mallei.
O-Polysaccharides (OPS) were isolated from purified Burkholderia pseudomallei and Burkholderia mallei lipopolysaccharides by mild-acid hydrolysis and gel-permeation chromatography. 1-D and 2-D (1)H and (13)C NMR spectroscopy experiments revealed that the OPS antigens were unbranched heteropolymers with the following structures: Collectively, our results demonstrate that the predominant OPS antigens expressed by B. pseudomallei and B. mallei isolates are structurally more complex than previously described and provide evidence that different capping residues are used by these closely related pathogens to terminate chain elongation. Additionally, they confirm that Burkholderia thailandensis and B. pseudomallei express OPS antigens that are essentially identical to one another.
Complex Carbohydrate Research Center, The University of Georgia, Athens, GA 30602, USA. Electronic address: cheiss@uga.edu.
Christian
C
Heiss
Mary N
MN
Burtnick
Rosemary A
RA
Roberts
Ian
I
Black
Parastoo
P
Azadi
Paul J
PJ
Brett
D042726:Burkholderia mallei / Q000737:chemistry*; D016957:Burkholderia pseudomallei / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002850:Chromatography, Gel; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification
D011134:Polysaccharides
Burkholderia mallei; Burkholderia pseudomallei; Burkholderia thailandensis; Lipopolysaccharide; O-polysaccharide; Structure