Comparison of heat inactivation and cell disruption protocols for identification of mycobacteria from solid culture media by use of vitek matrix-assisted laser desorption ionization-time of flight mass spectrometry.
Two novel protocols for inactivation and extraction were developed and used to identify 107 Mycobacterium clinical isolates, including Mycobacterium tuberculosis complex, from solid cultures using Vitek matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. The protocol using heat inactivation with sonication and cell disruption with glass beads resulted in 82.2% and 88.8% species and genus level identifications, respectively.
Emory University School of Medicine.
Alexandra
A
Machen
Miwako
M
Kobayashi
Mary Robin
MR
Connelly
Yun F Wayne
YF
Wang
D006358:Hot Temperature; D006801:Humans; D009161:Mycobacterium / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000528:radiation effects; D013010:Sonication; D013048:Specimen Handling / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology