Highly purified, multi-wall carbon nanotubes induce light-chain 3B expression in human lung cells.
Bronchial epithelial cells are targets of inhalation and play a critical role in the maintenance of mucosal integrity as mechanical barriers against various particles. Our previous result suggest that vapor-grown carbon fiber, HTT2800, which is one of the most highly purified multi-wall carbon nanotubes (MWCNT) showed cellular uptake of the carbon nanotube, increased cell death, enhanced DNA damage, and induced cytokine release. Increasing evidence suggests that autophagy may critically influence vital cellular processes such as apoptosis, cell proliferation and inflammation and thereby may play a critical role in pulmonary diseases. Autophagy was recently recognized as a critical cell death pathway, and autophagosome accumulation has been found to be associated with the exposure of various nanoparticles. In this study, the authors focus on the autophagic responses of HTT2800 exposure. The HTT2800-exposed cells induced LC3B expression and induced cell growth inhibition.
Department of Hematology and Immunology, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Ishikawa 920-0293, Japan. Electronic address: ttamotsu@kanazawa-med.ac.jp.
Tamotsu
T
Tsukahara
Yoshikazu
Y
Matsuda
Yuki
Y
Usui
Hisao
H
Haniu
D001343:Autophagy / Q000187:drug effects / Q000502:physiology*; D002244:Carbon / Q000633:toxicity*; D000077482:Carbon Fiber; D002460:Cell Line; D006801:Humans; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis*; D037742:Nanotubes, Carbon / Q000633:toxicity*
D000077482:Carbon Fiber; C502070:MAP1LC3B protein, human; D008869:Microtubule-Associated Proteins; D037742:Nanotubes, Carbon; D002244:Carbon
Autophagy; Bronchial epithelial cells; Carbon nanotube; LC3B