Elevated 3-hydroxypropionaldehyde production from glycerol using a Citrobacter freundii mutant.
A mutant strain of Citrobacter freundii capable of elevated 3-hydroxypropionaldehyde production from glycerol was isolated using chemical mutagenesis and a screening protocol. The protocol involved screening mutagenized bacterial cells on solid minimal medium containing 5 % (v/v) glycerol. Colonies were picked onto duplicate solid minimal medium plates and one plate was stained with 1 % (w/v) phloroglucinol. Those colonies staining red were further screened and a mutant, HPAO-1, was identified. The mutant strain produced a several-fold higher 3-hydroxypropionaldehyde concentration than did the parent strain when grown on 5 % (v/v) glycerol. The ratio of culture volume to flask volume influenced 3-hydroxypropionaldehyde production by the mutant cells compared to the parent cells. Aldehyde production was highest when the mutant strain was grown on 5 % (v/v) glycerol at a ratio of culture volume to flask volume of 1:3 or 1:12.5.
Department of Biology and Microbiology, South Dakota State University, Box 2104A, Brookings, SD, 57007, USA, thomas.west@sdstate.edu.
Thomas P
TP
West
Jessica L
JL
Peterson
D018533:Biomass; D016971:Citrobacter freundii / Q000235:genetics* / Q000378:metabolism; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism; D005985:Glyceraldehyde / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D009154:Mutation; D010696:Phloroglucinol / Q000737:chemistry / Q000378:metabolism; D011407:Propane / Q000032:analysis* / Q000378:metabolism*
D004396:Coloring Agents; C047158:3-hydroxypropionaldehyde; D005985:Glyceraldehyde; D010696:Phloroglucinol; D005990:Glycerol; D011407:Propane