Co-culture with neurotrophic factor secreting cells induced from adipose-derived stem cells: promotes neurogenic differentiation.
Adipose-derived stem cells (ADSCs) and bone marrow stem cells (BMSCs) can be equally proper in the treatment of neurodegenerative diseases. However, ADSCs have practical benefits. In this study, we attempted to induce the secretion of neurotrophic factors (NTF) in human ADSCs. We then evaluated the effects of co-culture with NTF secreting cells in neural differentiation of human ADSCs. Isolated human ADSCs were induced to neurotrophic factors secreting cells. To evaluate the in vitro effects of NTF-secreting ADSCs on neurogenic differentiation of ADSCs, we used neurogenic induction medium (control group), the combination of neurogenic medium and conditioned medium, or co-cultured NTF-secreting ADSCs which were encapsulated in alginate beads (co-culture) for 7 days. ELISA showed increased (by about 5 times) release of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in NTF-secreting ADSCs compared to human ADSCs. Real time RT-PCR analysis revealed that NTF-secreting ADSCs highly expressed NGF and BDNF. In addition, co-culture with NTF-secreting ADSCs could also promote neuronal differentiation relative to gliogenesis. Overall, NTF-secreting ADSCs secrete a range of growth factors whose levels in culture could promote neuronal differentiation and could support the survival and regeneration in a variety of neurodegenerative diseases.
Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan 81744-176, Iran. Electronic address: razavi@med.mui.ac.ir.
Shahnaz
S
Razavi
Mohamad Reza
MR
Razavi
Mahsa
M
Kheirollahi-Kouhestani
Mohammad
M
Mardani
Fatemeh Sadat
FS
Mostafavi
D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D002469:Cell Separation; D018920:Coculture Techniques; D005260:Female; D006801:Humans; D009414:Nerve Growth Factors / Q000378:metabolism*; D055495:Neurogenesis; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D055815:Young Adult
D009414:Nerve Growth Factors
Human adipose-derived stem cells; Neurodegenerative diseases; Neurogenesis; Neurotrophic factor secretion