The binding of okadaic acid analogs to recombinant OABP2.1 originally isolated from the marine sponge Halichondria okadai.
The binding between [24-(3)H]okadaic acid (OA) and a recombinant OA binding protein OABP2.1 was examined using various OA analog, including methyl okadaate, norokadanone, 7-deoxy OA, and 14,15-dihydro OA, 7-O-palmitoyl DTX1, to investigate the structure activity relationship. Among them, 7-O-palmitoyl DTX1, which is one of the diarrhetic shellfish poisoning (DSP) toxins identified in shellfish, displayed an IC50 for [24-(3)H]OA binding at 51±6.3nM (Mean±SD). In addition, a synthetic compound, N-pyrenylmethyl okadamide, exhibited its IC50 at 10±2.9nM (Mean±SD). These results suggested that the recombinant OABP2.1 and the N-pyrenylmethyl okadamide might be core substances in a novel assay for the DSP toxins.
Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan. Electronic address: konoki@m.tohoku.ac.jp.
Keiichi
K
Konoki
Tatsuya
T
Onoda
Sachie
S
Furumochi
Yuko
Y
Cho
Mari
M
Yotsu-Yamashita
Takeshi
T
Yasumoto
D000818:Animals; D008387:Marine Toxins / Q000737:chemistry* / Q000378:metabolism*; D019319:Okadaic Acid / Q000031:analogs & derivatives* / Q000378:metabolism*; D011161:Porifera / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism
D008387:Marine Toxins; D011994:Recombinant Proteins; D019319:Okadaic Acid
Diarrhetic shellfish poisoning; Dinophysistoxins; Okadaic acid; Okadaic acid binding protein