IS6110-based differentiation of Mycobacterium tuberculosis strains.
In this study, 62 Mycobacterium tuberculosis strains were characterized by fast ligation-mediated PCR (FLiP) and, previously performed, IS6110 restriction fragment length polymorphism (RFLP). FLiP proved a reproducible and specific method for differentiation between M. tuberculosis strains. The discriminatory power of FLiP was close to that of the reference IS6110 RFLP suggesting its usefulness in studying the genetic diversity of M. tuberculosis strains.
Department of Biochemistry and Cell Biology, Faculty of Biology and Agriculture, University of Rzeszów, Cwiklińskiej 2, 35-601 Rzeszów, Poland.
Anna
A
Zaczek
Małgorzata
M
Ziółkiewicz
Arkadiusz
A
Wojtasik
Jarosław
J
Dziadek
Anna
A
Sajduda
D001483:Base Sequence; D016000:Cluster Analysis; D015964:Gene Expression Regulation, Bacterial; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D059467:Transcriptome