Effect of rCaIFN-γ on NK cytotoxic activity in the peripheral blood of dogs.
Previously, the ability of interferon (IFN) to reinforce antitumor immune capacity has received much attention. In humans and mice, natural killer (NK) cells are activated by IFN, thereby reinforcing antitumor immunity. We investigated whether NK cytotoxic activity can be enhanced by recombinant canine interferon-gamma (rCaIFN-γ) in dogs. First, we investigated the effects of various concentrations of and time exposures to IFN-γ in the culture medium on the NK cytotoxic activity of peripheral blood lymphocyte (PBLs) extracted from healthy beagles. Time- and concentration-dependent enhancement of NK cytotoxic activity of PBLs was observed. We then investigated whether the NK cytotoxic activity of PBLs is enhanced 24h after administration of rCaIFN-γ (10,000 units/kg body weight) in healthy beagles. Our in vivo study confirmed that NK cytotoxic activity of PBLs was enhanced by this approach, suggesting that antitumor immunity was reinforced. In dogs, rCaIFN-γ may be effective for bolstering antitumor immune capacity.
Division of Veterinary Surgery, Department of Veterinary Science, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan. Electronic address: sur.miyata@gmail.com.
Takuma
T
Miyata
Keiichi
K
Hashimoto
Kenji
K
Miura
Rie
R
Honma
Takanori
T
Kodama
Yoshinori
Y
Nezu
Yasuji
Y
Harada
Takuya
T
Yogo
Yasushi
Y
Hara
Masahiro
M
Tagawa
D000818:Animals; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D008297:Male; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors
D011994:Recombinant Proteins; D007371:Interferon-gamma
Dog; IFN-γ; NK cytotoxic activity