Rapid trace level determination of sulfonamide residues in honey with online extraction using short C-18 column by high-performance liquid chromatography with fluorescence detection.
A sensitive and inexpensive quantification method with online extraction using a short C-18 column for sulfonamide residues in honey by high performance liquid chromatography with fluorescence detector was developed and validated. In sample preparation, acid hydrolysis was used to break the N-glycoside bond between the honey sugar and sulfonamide drugs and derivatization of sulfonamide residues with fluorescamine was conducted at pH 3.5 using a citrate buffer (0.5M) in the honey matrix. The chromatography was carried out on Zorbax Extended C-18 (250mm×4.6mm; 5μm) column, using a mixture of acetonitrile and an acetate buffer (pH 4.50, 20mM) as a mobile phase. A Zorbax Extended C-18 (12mm×4.6mm; 5μm) column was used for online extraction of fifteen sulfonamide residues from honey sample with the help of a two position valve. The limit of quantification of sulfonamide residues in honey was less than 3ngg(-1), and the percentage recovery of study compounds in spiked honey sample was from 80% for sulfacetamide to 100% of sulfachloropyridazine. The developed method has excellent linearity for all studied sulfonamides with a correlation coefficient 0.993.
College of Chemistry, Beijing Normal University, Beijing 100875, China.
Muhammad
M
Sajid
Na
N
Na
Muhammad
M
Safdar
Xin
X
Lu
Lin
L
Ma
Lan
L
He
Jin
J
Ouyang
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D006722:Honey / Q000032:analysis*; D012015:Reference Standards; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013449:Sulfonamides / Q000032:analysis*
D013449:Sulfonamides
C-18 extraction; HPLC with fluorescence detection; Honey; Large volume injection; Online extraction; Sulfonamide