Identification of TET1 Partners That Control Its DNA-Demethylating Function.
Several recent reports have identified TET1 as the main enzyme modulating DNA methylation and gene transcription via hydroxylation of 5-methylcytosine. However, little is known about the protein network that controls TET1 activity. By using a new proximity ligation in situ assay, we identified MeCP2, HDAC1/6/7, EZH2, mSin3A, PCNA, and LSD1 as TET1-interacting proteins. We also discerned that TET1/PCNA acts as a demethylator of the cyclical methylation/demethylation process, the perturbation of which promotes the aberrant methylation hallmarks frequently observed in cancer cells.
Equipe Apoptose et Progression Tumorale, Centre de Recherche en Cancérologie Nantes-Angers, INSERM U892, Nantes, France ; Département de Recherche en Cancérologie, Faculté de Médecine, Université de Nantes, Nantes, France.
Pierre-François
PF
Cartron
Arulraj
A
Nadaradjane
Fiona
F
Lepape
Lisenn
L
Lalier
Betty
B
Gardie
François M
FM
Vallette
DNA methylation; TET1; demethylation; glioma