Characterization of a bisphenol A specific yeast bioreporter utilizing the bisphenol A-targeted receptor.
The first Saccharomyces cerevisiae yeast bioreporter for analysis of a single endocrine disrupting compound, bisphenol A (BPA), was developed. The bioreporter contains mutated human estrogen receptor α (hERα), called bisphenol A-targeted receptor (BPA-R). The BPA-R bioreporter was characterized with mixtures of estrogenic chemicals and tested with spiked influent wastewater samples. The detection limit for BPA was 4.2-fold lower (0.107 μM, i.e., 24 μg L(-1)), while that of the native hormone 17β-estradiol (E2) (1 μM, i.e., 272 μg L(-1)) was 166,000-fold higher compared to the wild type hERα bioreporter. The BPA-R bioreporter responded only to BPA in a chemical cocktail and spiked concentrated wastewater samples with high concentrations of other estrogenic chemicals. As a conclusion, wastewater and other environmental water samples can be concentrated and specifically analyzed for BPA without risk of the mixture effect caused by other estrogenic chemicals. The BPA-R bioreporter is a robust and cost-efficient choice for high-throughput monitoring of BPA and its bioavailability in complex samples.
Department of Food and Environmental Sciences, University of Helsinki , Finland.
Johanna
J
Rajasärkkä
Marko
M
Virta
D001559:Benzhydryl Compounds / Q000737:chemistry*; D047628:Estrogen Receptor alpha / Q000032:analysis*; D006801:Humans; D057230:Limit of Detection; D010636:Phenols / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D047628:Estrogen Receptor alpha; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds