Adenovirus conducted connective tissue growth factor on extracellular matrix in trabecular meshwork and its role on aqueous humor outflow facility.
Deposition of extracellular matrix (ECM) in trabecular meshwork, such as fibronectin, collagen IV, elastin. leads to increased resistance of trabecular meshwork in primary open angle glaucoma (POAG). Connective tissue growth factor (CTGF) is known to regulate the ECM deposits. In this study, we detect the effect of adenovirus conducted CTGF (Adv-CTGF) transfection on either the expression of ECM components or aqueous humor outflow facility. Adv-CTGF was used to transfect rat trabecular meshwork cells in vivo and in vitro. Aqueous humor outflow facility was test by microbeads perfusion. Protein expression of CTGF, fibronectin, and collagen IV was determined using Western blot. In the Adv-CTGF group, the outflow facility displayed a significant decrease from baseline. It appears as though the transfection with Adv-CTGF significantly affects the aqueous humor outflow pattern. A negative correlation between IOP and PEFL indicated that a decrease in the area of bead deposition corresponded to an overall decrease of outflow, leading to an elevated IOP. Adv-CTGF can enhance the expression of CTGF, fibronectin and collagen IV. CTGF is the novel target for treatment of POAG. It is necessary to further study to test inhibition of CTGF expression for treatment of POAG.
Department of Ophthalmology, First Clinical College of Harbin Medical University, Harbin, 150001, China.
Ying
Y
Su
Jingli
J
Cheng
Hongtao
H
Liu
Feng
F
Wang
Shiguang
S
Zhao
D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D002460:Cell Line; D024141:Collagen Type IV / Q000235:genetics / Q000378:metabolism; D055513:Connective Tissue Growth Factor / Q000235:genetics* / Q000378:metabolism; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000378:metabolism*; D005123:Eye / Q000378:metabolism / Q000503:physiopathology; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics*; D005902:Glaucoma, Open-Angle / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051381:Rats; D014129:Trabecular Meshwork / Q000378:metabolism*; D014162:Transfection
D024141:Collagen Type IV; D005353:Fibronectins; D055513:Connective Tissue Growth Factor