Antiviral activity of methyl helicterate isolated from Helicteres angustifolia (Sterculiaceae) against hepatitis B virus.
The anti-HBV effect of methyl helicterate (MH), a triterpenoid isolated from the Chinese herb Helicteres angustifolia, was explored both in vitro and in vivo. In the HBV-transfected cell line HepG2.2.15, the secretion of HBsAg/HBeAg, the levels of HBV DNA and cccDNA, and the amount of viral RNA were significantly decreased after treatment with MH for 144h. In addition, MH had no inhibitory effect on the mitochondrial DNA content. In DHBV-infected ducklings, MH significantly reduced the serum DHBV DNA, liver total viral DNA, and cccDNA levels. Furthermore, analysis of the liver pathological changes confirmed the hepatoprotective effect of MH. These results indicate that MH efficiently inhibits HBV replication both in vitro and in vivo and that MH may be a major bioactive ingredient in H. angustifolia.
The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China.
Quanfang
Q
Huang
Renbin
R
Huang
Ling
L
Wei
Yongxing
Y
Chen
Shujuan
S
Lv
Chunhong
C
Liang
Xuerong
X
Zhang
Fujiang
F
Yin
Hongtao
H
Li
Lang
L
Zhuo
Xing
X
Lin
D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D004372:Ducks; D018347:Hepadnaviridae Infections / Q000188:drug therapy; D015611:Hepatitis B Virus, Duck / Q000235:genetics / Q000302:isolation & purification; D006515:Hepatitis B virus / Q000187:drug effects*; D006524:Hepatitis, Viral, Animal / Q000188:drug therapy*; D022781:Hepatocytes / Q000821:virology; D006651:Histocytochemistry; D006801:Humans; D008099:Liver / Q000473:pathology / Q000821:virology; D019660:Malvaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D011201:Poultry Diseases / Q000188:drug therapy; D044967:Serum / Q000821:virology; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D019562:Viral Load
D000998:Antiviral Agents; D004279:DNA, Viral; D010936:Plant Extracts; D014315:Triterpenes; C049014:methyl helicterate
Antiviral activity; Helicteres angustifolia; Hepatitis B virus; Methyl helicterate