Sulfation of β-chitosan and evaluation of biological activity from gladius of Sepioteuthis lessoniana.
β-Chitosan extracted from the gladius of Sepioteuthis lessoniana was sulfated with chlorosufonic acid. The structural features of sulfated chitosan were analyzed by NMR spectroscopy. The elemental composition was analyzed using elemental analyzer. The molecular weight of sulfated chitosan estimated through viscometry was calculated as 12.47 × 10(4) Da. The water solubility of sulfated chitosan was found to be 81%. The sulfated chitosan was able to scavenge the DPPH, superoxide and hydroxyl radicals and the inhibitory concentration (IC50) of those free radicals was found to be 4, 0.428 and 0.473 mg/ml respectively. Sulfated chitosan also showed reducing and chelating property. The IC50 value of reducing and chelating ability was found to be 0.316 and 5.64 mg/ml respectively. The anticoagulant activity was determined for human plasma with respect to activated partial thromboplastin time and prothrombin time and it was 7.13 IU and 1.26 IU, respectively.
CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608502, Tamil Nadu, India.
Namasivayam
N
Subhapradha
Pasiyappazham
P
Ramasamy
Alagiri
A
Srinivasan
Perumal
P
Madeswaran
Vairamani
V
Shanmugam
Annaian
A
Shanmugam
D000818:Animals; D000925:Anticoagulants / Q000737:chemistry* / Q000494:pharmacology*; D049830:Cephalopoda / Q000033:anatomy & histology*; D002614:Chelating Agents / Q000737:chemistry / Q000494:pharmacology; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000494:pharmacology*; D005609:Free Radicals / Q000737:chemistry; D006801:Humans; D007501:Iron / Q000737:chemistry; D008970:Molecular Weight; D010314:Partial Thromboplastin Time; D011517:Prothrombin Time; D012995:Solubility; D014867:Water / Q000737:chemistry
D000925:Anticoagulants; D002614:Chelating Agents; D016166:Free Radical Scavengers; D005609:Free Radicals; C480355:chitosan sulfate; D014867:Water; D048271:Chitosan; D007501:Iron
Anticoagulant activity; Antioxidant activity; Sepioteuthis lessoniana; Structural analysis; Sulfated chitosan