Role of phospholemman and the 70 kDa inhibitor protein in regulating Na+/K+ ATPase activity in pulmonary artery smooth muscle cells under U46619 stimulation.
Treatment of bovine pulmonary smooth muscle cells with U46619 inhibited the Na(+)/K(+) ATPase activity in two parallel pathways: one of which is mediated via glutathionylation of the pump and the other by augmenting the inhibitory activity of the 70kDa inhibitor protein of Na(+)/K(+) ATPase. Although phospholemman deglutathionylates the pump leading to its activation, the inhibitor is responsible for irreversible inhibition of Na(+)/K(+) ATPase in an isoform specific manner during treatment of the cells with U46619.
Department of Biochemistry and Biophysics, University of Kalyani, Kalyani 741235, West Bengal, India.
Kuntal
K
Dey
Sayed Modinur
SM
Rahaman
Tapati
T
Chakraborti
Sajal
S
Chakraborti
D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D008565:Membrane Proteins / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D011651:Pulmonary Artery / Q000201:enzymology* / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D014662:Vasoconstrictor Agents / Q000494:pharmacology*
D008565:Membrane Proteins; D010750:Phosphoproteins; D020033:Protein Isoforms; D014662:Vasoconstrictor Agents; C069141:phospholemman; D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D000254:Sodium-Potassium-Exchanging ATPase
Inhibitor protein; Na(+)/K(+) ATPase; Phospholemman; Protein kinase; TxA(2); U46619