[Effect of 3-bromopyruvate on mitochondrial membrane potential and apoptosis of human breast carcinoma SK-BR-3 cells].
OBJECTIVE
To study the effect of glycolysis inhibitor 3-bromopyruvate (3-BrPA) in inducing apoptosis of human breast carcinoma cells SK-BR-3 and the possible mechanism.


METHODS
MTT assay was used to detect the growth inhibition induced by 3-BrPA in breast cancer cells SK-BR-3. The apoptotic cells were detected by flow cytometry with propidium iodide (PI). ATP levels in the cells were detected by ATP assay kit, and DHE fluorescent probe technique was used to determine superoxide anion levels; the mitochondrial membrane potential was assessed using JC-1 staining assay.


RESULTS
MTT assay showed that the proliferation of SK-BR-3 cells was inhibited by 3-BrPA in a time- and concentration-dependent manner. Exposure to 80, 160, and 320 µmol·L(-1) 3-BrPA for 24 h resulted in cell apoptosis rates of 6.7%, 22.3%, and 79.6%, respectively, and the intracellular ATP levels of SK-BR-3 cells treated with 80, 160, 320 µmol·L(-1) 3-BrPA for 5 h were 87.7%, 60.6%, and 23.7% of the control levels. 3-BrPA at 160 µmol·L(-1) increased reactive oxygen levels and lowered mitochondrial membrane potential of SK-BR-3 cells.


CONCLUSIONS
3-BrPA can inhibit cell proliferation, reduce the mitochondrial membrane potential and induce apoptosis in SK-BR-3 cells, the mechanism of which may involve a reduced ATP level by inhibiting glycolysis and increasing the reactive oxygen level in the cells.
Department of Pharmacy, Anhui Bengbu Medical College, Biochemical Drugs Engineering and Technological Research Center of Anhui Province, Bengbu 233030, China.E-mail: keeryuanyuan@126.com.
Yuanyuan
Y
Zhang
Zhe
Z
Liu
Qianwen
Q
Zhang
Zhenhua
Z
Chao
Pei
P
Zhang
Fei
F
Xia
Chenchen
C
Jiang
Hao
H
Liu
Zhiwen
Z
Jiang
D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D005260:Female; D006019:Glycolysis; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D011773:Pyruvates / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
D011773:Pyruvates; D017382:Reactive Oxygen Species; C017092:bromopyruvate