A cancer vaccine based on the marine antimicrobial peptide pardaxin (GE33) for control of bladder-associated tumors.
The marine antimicrobial peptide (AMP) GE33, also known as pardaxin, possesses antimicrobial and anticancer properties, and modulates host signaling. GE33 has cytotoxic effects on murine bladder carcinoma (MBT-2) cells. Here, we investigated the potential of GE33 combined with inactivated MBT-2 as a cancer vaccine. The presence of up to 12.5 μg of GE33 did not inhibit the proliferation or endogenous nitrous oxide (NO) levels of RAW264.7 cells. However, the secretion of MCP-1, IL-6, and IL-12 by RAW264.7 cells was affected by GE33. We proceeded to test the effectiveness of the vaccine by immunizing mice at 7, 14, and 21 days of age, and injecting live MBT-2 cells on the 28th day. Tumor growth by the 58th day was attenuated in mice treated with the vaccine, as compared to the control group. Induction of MBT-2 specific-tumor antigens was increased in mice immunized with our vaccine. Furthermore, activation of T-cell receptors, cytotoxic T-cells, and NK cells was enhanced, and these showed high specificity for targeting tumor cells. Finally, immunization controlled excess recruitment of monocytes, lymphocytes, T-helper cells, and NK cells, and decreased the expression of VEGF. This report provides empirical evidence that our GE33-based vaccine enhances antitumor immunity in mice.
Department of Food Science, National Taiwan Ocean University, 2, Pei-Ning Road, Keelung, Taiwan.
Han-Ning
HN
Huang
Venugopal
V
Rajanbabu
Chieh-Yu
CY
Pan
Yi-Lin
YL
Chan
Chang-Jer
CJ
Wu
Jyh-Yih
JY
Chen
D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005397:Fish Venoms; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D051379:Mice; D001749:Urinary Bladder Neoplasms / Q000276:immunology* / Q000378:metabolism / Q000517:prevention & control*
D019496:Cancer Vaccines; D005397:Fish Venoms; D015850:Interleukin-6; C017603:pardaxin
Antimicrobial peptide; Bladder tumor; Cancer vaccine; Cytokines; MBT-2; Pardaxin