Linear fusigen as the major hydroxamate siderophore of the ectomycorrhizal Basidiomycota Laccaria laccata and Laccaria bicolor.
A screening for siderophores produced by the ectomycorrhizal fungi Laccaria laccata and Laccaria bicolor in synthetic low iron medium revealed the release of several different hydroxamate siderophores of which four major siderophores could be identified by high resolution mass spectrometry. While ferricrocin, coprogen and triacetylfusarinine C were assigned as well as other known fungal siderophores, a major peak of the siderophore mixture revealed an average molecular mass of 797 for the iron-loaded compound. High resolution mass spectrometry indicated an absolute mass of m/z = 798.30973 ([M + H](+)). With a relative error of Δ = 0.56 ppm this corresponds to linear fusigen (C33H52N6O13Fe; MW = 797.3). The production of large amounts of linear fusigen by these basidiomycetous mycorrhizal fungi may possibly explain the observed suppression of plant pathogenic Fusarium species. For comparative purposes Fusarium roseum was included in this study as a well known producer of cyclic and linear fusigen.
Department of Microbiology, University of Innsbruck, Technikerstr. 25, 6020, Innsbruck, Austria.
Kurt
K
Haselwandter
Gerlinde
G
Häninger
Markus
M
Ganzera
Hubertus
H
Haas
Graeme
G
Nicholson
Günther
G
Winkelmann
D000898:Antibiosis; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D005290:Ferric Compounds / Q000302:isolation & purification / Q000378:metabolism*; D005291:Ferrichrome / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000378:metabolism; D005670:Fusarium / Q000254:growth & development; D006877:Hydroxamic Acids / Q000302:isolation & purification / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D055399:Laccaria / Q000254:growth & development / Q000378:metabolism*; D013058:Mass Spectrometry; D008970:Molecular Weight; D018517:Plant Roots / Q000382:microbiology; D017262:Siderophores / Q000302:isolation & purification / Q000378:metabolism*; D064028:Tracheophyta / Q000382:microbiology
D003470:Culture Media; D005290:Ferric Compounds; D006877:Hydroxamic Acids; D017262:Siderophores; D005291:Ferrichrome; C010966:fusigen; C021678:ferricrocin; C053324:N,N',N''-triacetylfusarinine C; C010896:coprogen; D007501:Iron