Effects of Passiflora nitida Kunth leaf extract on digestive enzymes and high caloric diet in rats.
The present study investigated inhibition of pancreatic lipase and metabolic effects of high caloric diet in rats. The Passiflora nitida hydroethanol leaf extract (PNE) was used in in vitro assays or administered to rats to study dyslipidemia. Inhibition of lipase in vitro was studied by a spectrophotometric assay using orlistat as the positive control. The effects of PNE on reduction of postprandial triglyceride were studied by oral fat-overloading in rats. Metabolic alterations were induced using the cafeteria diet and 4 weeks post-treatment with PNE or orlistat and blood samples were collected and biochemical analyses were performed. Liver and retroperitoneal fat tissues were obtained to analyze weight and steatosis. IC50 (lg/mL) values for pancreatic lipase inhibition were 21.2 ± 0.8 and 0.1 ± 0.01 for PNE and orlistat, respectively. Oral administration of lipid emulsion resulted in postprandial hypertriglyceridemia at 3 h postadministration and when rats were then administered PNE and orlistat there was decreased of triglyceride levels by 15 % compared to control. Although the energy consumption by the cafeteria diet had been higher, there was no significant weight gain observed in the study groups. The cafeteria diet resulted in a significant increase of weight in the retroperitoneal fat and hypertriglyceridemia levels that could be significantly reduced by PNE and orlistat treatment. We hypothesized that PNE administration prevented the hypertriglyceridemia in rats with a high caloric diet, possibly owing to reduction of lipid absorption and pancreatic lipase inhibition.
Lorisa S
LS
Teixeira
Arleilson S
AS
Lima
Ana Paula A
AP
Boleti
Adley A N
AA
Lima
Said T
ST
Libório
Lucia
L
de Paula
Maria Inês B
MI
Oliveira
Everton F
EF
Lima
Geison M
GM
Costa
Flávio H
FH
Reginatto
Emerson S
ES
Lima
D000273:Adipose Tissue / Q000473:pathology; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004032:Diet / Q000009:adverse effects; D002149:Energy Intake; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015228:Hypertriglyceridemia / Q000097:blood / Q000209:etiology / Q000517:prevention & control*; D007783:Lactones / Q000494:pharmacology; D008049:Lipase / Q000037:antagonists & inhibitors*; D008055:Lipids / Q000008:administration & dosage; D008099:Liver / Q000473:pathology; D008297:Male; D000077403:Orlistat; D029598:Passiflora / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood
D004791:Enzyme Inhibitors; D007783:Lactones; D008055:Lipids; D010936:Plant Extracts; D014280:Triglycerides; D000077403:Orlistat; D008049:Lipase