Structure-activity relationship of carbamate-linked cationic lipids bearing hydroxyethyl headgroup for gene delivery.
A novel series of carbamate-linked cationic lipids containing hydroxyl headgroup were synthesized and included in formulations for transfection assays. The DNA-lipid complexes were characterized for their ability to bind DNA, their size, ζ-potential and cytotoxicity. Compared with our previously reported cationic transfection lipid DDCDMA lacking the hydroxyl group and the commercially available, these cationic liposomes exhibited relatively higher transfection efficiency.
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China; SEAC-ME Key Laboratory of Biochemistry Engineering, Dalian Nationalities University, Dalian 116600, China.
Defu
D
Zhi
Shubiao
S
Zhang
Farooq
F
Qureshi
Yinan
Y
Zhao
Shaohui
S
Cui
Bing
B
Wang
Huiying
H
Chen
Baoling
B
Yang
Defeng
D
Zhao
D002219:Carbamates / Q000737:chemistry; D002412:Cations; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics*; D018014:Gene Transfer Techniques; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry* / Q000494:pharmacology*; D008081:Liposomes / Q000008:administration & dosage / Q000737:chemistry; D013329:Structure-Activity Relationship; D014162:Transfection
D002219:Carbamates; D002412:Cations; D008055:Lipids; D008081:Liposomes; D004247:DNA
Carbamate-linked cationic lipids; Cytotoxicity; Gene delivery; Hydroxyl group; Transfection efficiency