Serological studies of Proteus penneri strains determining qualification to appropriate O-serogroup.
Our Department of General Microbiology created a wide collection of P. penneri isolates and classified most of them into 19 different O-serogroups. This work describes the classification of 12 remaining P. penneri strains. The lipopolysaccharides extracted from P. penneri strains were tested in an enzyme-linked immunosorbent assay (ELISA) with selected O-antisera against Proteus sp. strains. Homologous and cross-reacting systems were checked in: passive immunohemolysis (PIH), inhibition of ELISA and PIH and Western blot procedure. These studies led to the qualification of tested P. penneri strains to five Proteus sp. O-serogroups, thus completing the serological classification of the whole collection.
Department of General Microbiology, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, Poland. agatapal@biol.uni.lodz.pl
Agata
A
Palusiak
Małgorzata
M
Siwińska
Zygmunt
Z
Sidorczyk
D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008070:Lipopolysaccharides / Q000145:classification / Q000378:metabolism; D044095:Proteus penneri / Q000145:classification*; D012703:Serotyping
D008070:Lipopolysaccharides