[Preparation of ferulic acid, senkyunolide I and senkyunolide H from Ligusticum chuanxiong by preparative HPLC].
Preparative HPLC was used to prepare ferulic acid, senkyunolide I and senkyunolide H from Ligusticum chuanxiong. The separation was conducted on a Shim-Pack Prep-ODS (20.0 mm x 250 mm, 5 microm) column with the mobile phase of methanol-0.2% glacial acetic acid (50:50)at the flow rate of 5 mL x min(-1). The detection wavelength was 278 nm, and the purity of each compound was detected by HPLC analysis. Spectral data analyses including UV, ESI-MS and NMR were used to identify their structures. This method is simple, fast, which is suitable for preparation of standard reference of ferulic acid, senkyunolide I and senkyunolide H from L. chuanxiong and can meet the requirement of new drug research and development.
Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Yao-Kun
YK
Xiong
Shuang
S
Liang
Yan-Long
YL
Hong
Xiu-Juan
XJ
Yang
Lan
L
Shen
Yan
Y
Du
Yi
Y
Feng
D001572:Benzofurans / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000737:chemistry / Q000302:isolation & purification*; D030004:Ligusticum / Q000737:chemistry*
D001572:Benzofurans; D003373:Coumaric Acids; C584604:senkyunolide H; C576743:senkyunolide I; C004999:ferulic acid