Expression of aquaporin8 in human astrocytomas: correlation with pathologic grade.
Aquaporin8 (AQP8), a member of the aquaporin (AQP) protein family, is weakly distributed in mammalian brains. Previous studies on AQP8 have focused mainly on the digestive and the reproductive systems. AQP8 has a pivotal role in keeping the fluid and electrolyte balance. In this study, we investigated the expression changes of AQP8 in 75 cases of human brain astrocytic tumors using immunohistochemistry, Western blotting, and reverse transcription polymerase chain reaction. The results demonstrated that AQP8 was mainly distributed in the cytoplasm of astrocytoma cells. The expression levels and immunoreactive score of AQP8 protein and mRNA increased in low-grade astrocytomas, and further increased in high-grade astrocytomas, especially in glioblastoma. Therefore, AQP8 may contribute to the proliferation of astrocytomas, and may be a biomarker and candidate therapy target for patients with astrocytomas.
Institute of Neurobiological Sciences, Faculty of Basic Medical Science of Chongqing Medical University, Medical College Road 1, YuanJiaGang, YuZhong District, Chongqing 400016, People's Republic of China.
Shu-Juan
SJ
Zhu
Ke-Jian
KJ
Wang
Sheng-Wei
SW
Gan
Jin
J
Xu
Shi-Ye
SY
Xu
Shan-Quan
SQ
Sun
D000293:Adolescent; D000328:Adult; D000368:Aged; D020346:Aquaporins / Q000032:analysis / Q000235:genetics*; D001254:Astrocytoma / Q000235:genetics* / Q000473:pathology*; D001921:Brain / Q000378:metabolism / Q000473:pathology*; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics; D055815:Young Adult
D020346:Aquaporins; D012333:RNA, Messenger; C108330:aquaporin 8
Aquaporin-8; Astrocytic tumors; Pathologic grade