Rhizobium laguerreae sp. nov. nodulates Vicia faba on several continents.
Several fast-growing strains nodulating Vicia faba in Peru, Spain and Tunisia formed a cluster related to Rhizobium leguminosarum. The 16S rRNA gene sequences were identical to that of R. leguminosarum USDA 2370(T), whereas rpoB, recA and atpD gene sequences were phylogenetically distant, with sequence similarities of less than 96 %, 97 % and 94 %, respectively. DNA-DNA hybridization analysis showed a mean relatedness value of 43 % between strain FB206(T) and R. leguminosarum USDA 2370(T). Phenotypic characteristics of the novel strains also differed from those of the closest related species of the genus Rhizobium. Therefore, based on genotypic and phenotypic data obtained in this study, we propose to classify this group of strains nodulating Vicia faba as a novel species of the genus Rhizobium named Rhizobium laguerreae sp. nov. The type strain is FB206(T) ( = LMG 27434(T) = CECT 8280(T)).
Laboratory of Legumes, Centre of Biotechnology of Borj-Cédria, BP 901, Hammam-lif 2050, Tunisia.
Sabrine
S
Saïdi
Unidad Asociada Grupo de Interacción Planta-Microorganismo Universidad de Salamanca-IRNASA-CSIC.|Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), Salamanca, Spain.
Martha-Helena
MH
Ramírez-Bahena
Laboratorio de Rhizobiología, Dpto de Agronomía y Zootecnia, Universidad Nacional de San Cristóbal de Huamanga, Peru.
Nery
N
Santillana
Laboratorio de Ecología Microbiana y Biotecnología Marino Tabusso, Dpto. de Biología. Universidad Nacional Agraria La Molina, Lima, Peru.
Doris
D
Zúñiga
Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), Salamanca, Spain.
Estela
E
Álvarez-Martínez
Unidad Asociada Grupo de Interacción Planta-Microorganismo Universidad de Salamanca-IRNASA-CSIC.|Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), Salamanca, Spain.
Alvaro
A
Peix
Laboratory of Legumes, Centre of Biotechnology of Borj-Cédria, BP 901, Hammam-lif 2050, Tunisia.
Ridha
R
Mhamdi
Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.|Unidad Asociada Grupo de Interacción Planta-Microorganismo Universidad de Salamanca-IRNASA-CSIC.
Encarna
E
Velázquez
D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010568:Peru; D010641:Phenotype; D010802:Phylogeny; D055170:Plant Root Nodulation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000502:physiology; D013030:Spain; D013559:Symbiosis; D014416:Tunisia; D031307:Vicia faba / Q000382:microbiology*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S