Differential expression of alpha II spectrin in monocytes of tuberculosis patients.
Monocytes play a crucial role in immune response to tuberculosis. The present study focuses on identifying differences in the monocyte proteome profile of tuberculosis patients, household contacts and healthy controls. Differential protein expression was studied by two-dimensional (2D) gel electrophoresis. One of the spots consistently showed either lower intensity or was absent in patients and was identified as alpha II-spectrin. The decreased expression of αII-spectrin was further validated by quantitative PCR (qPCR) and western blot analysis. This study suggests the possible role of decreased levels of αII-spectrin in the pathology of tuberculosis.
Department of Microbiology and Biotechnology Centre, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara 390002, India.
Parul
P
Shrivastava
Tamishraha
T
Bagchi
D000328:Adult; D005260:Female; D006801:Humans; D008297:Male; D009000:Monocytes / Q000378:metabolism*; D040901:Proteomics; D013049:Spectrin / Q000235:genetics / Q000378:metabolism*; D014397:Tuberculosis, Pulmonary / Q000378:metabolism*
D013049:Spectrin
2-D; 2-dimensional gel electrophoresis; AFB; EDTA; HHC; HRP; IPG; MTB; Monocytes; Mycobacterium tuberculosis; PBMCs; PBS; Proteome; acid fast bacilli; ethylene diamine tetraacetic acid; horseradish peroxidase; household contacts; immobilized pH gradient strips; peripheral blood mononuclear cells; phosphate buffered saline; qPCR; quantitative PCR; αII-spectrin