DHTKD1 is essential for mitochondrial biogenesis and function maintenance.
Maintaining the functional integrity of mitochondria is crucial for cell function, signal transduction and overall cell activities. Mitochondrial dysfunctions may alter energy metabolism and in many cases are associated with neurological diseases. Recent studies have reported that mutations in dehydrogenase E1 and transketolase domain-containing 1 (DHTKD1), a mitochondrial protein encoding gene, could cause neurological abnormalities. However, the function of DHTKD1 in mitochondria remains unknown. Here, we report a strong correlation of DHTKD1 expression level with ATP production, revealing the fact that DHTKD1 plays a critical role in energy production in mitochondria. Moreover, suppression of DHTKD1 leads to impaired mitochondrial biogenesis and increased reactive oxygen species (ROS), thus leading to retarded cell growth and increased cell apoptosis. These findings demonstrate that DHTKD1 contributes to mitochondrial biogenesis and function maintenance.
Department of Clinical Laboratories, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai 200011, China.
Wangyang
W
Xu
Houbao
H
Zhu
Mingmin
M
Gu
Qingqiong
Q
Luo
Jieying
J
Ding
Yuting
Y
Yao
Fuxiang
F
Chen
Zhugang
Z
Wang
D000255:Adenosine Triphosphate / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000378:metabolism; D006801:Humans; D007655:Ketoglutarate Dehydrogenase Complex; D007658:Ketone Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D063269:Mitochondrial Turnover; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
D004272:DNA, Mitochondrial; D017382:Reactive Oxygen Species; D000255:Adenosine Triphosphate; D007658:Ketone Oxidoreductases; C578281:DHTKD1 protein, human; D007655:Ketoglutarate Dehydrogenase Complex
Cell apoptosis; DHTKD1; Mitochondrial dysfunction; ROS; mtDNA