Immunohistochemistry and RNA in situ hybridization in mouse brain development.
During development, the mouse brain is progressively divided into functionally distinct compartments. Numerous neuronal and glial cell types are subsequently generated in response to various inductive signals. Each cell expresses a unique combination of genes encoding proteins from transcription factors to neurotransmitters that define its role in brain function. To understand these important and highly sophisticated processes, it is critical to accurately locate the various proteins and cells that produce them. In this chapter, we introduce the techniques of immunohistochemistry, which detects the localization of specific proteins, and RNA in situ hybridization, which enables the visualization of specific mRNAs.
Department of Biology, Eberly College of Science, Center for Cellular Dynamics, Huck Institute of Life Sciences, The Penn State University, University Park, PA, USA.
Jinling
J
Liu
Aimin
A
Liu
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D016616:Cryoultramicrotomy; D004076:Digoxigenin / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods*; D017403:In Situ Hybridization / Q000379:methods*; D051379:Mice; D015347:RNA Probes / Q000737:chemistry* / Q000378:metabolism
D015347:RNA Probes; D000469:Alkaline Phosphatase; D004076:Digoxigenin