Gp10 based-thioetherification (10BASE(d)-T) on a displaying library peptide of bacteriophage T7.
The site-specific introduction of a haloacetamide derivative into a designated cysteine on a displaying peptide on a capsid protein (gp10) of bacteriophage T7 has been achieved. This easiest gp10-based thioetherification (10BASEd-T) is carried out in one-pot without side reactions or loss of phage infectivity.
Department of Engineering Science, Bioscience and Technology Program, The Graduate School of Informatics and Engineering, The University of Electro-Communications (UEC), 1-5-1 Chofugaoka, Chofu, Tokyo 182-8585, Japan. taki@pc.uec.ac.jp.
Keisuke
K
Fukunaga
Takaaki
T
Hatanaka
Yuji
Y
Ito
Masumi
M
Taki
D000595:Amino Acid Sequence; D017123:Bacteriophage T7 / Q000378:metabolism*; D036022:Capsid Proteins / Q000737:chemistry* / Q000378:metabolism; D019151:Peptide Library; D011499:Protein Processing, Post-Translational; D012235:Rhodamines / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry*
D036022:Capsid Proteins; D019151:Peptide Library; D012235:Rhodamines; D013440:Sulfides; C005358:tetramethylrhodamine