Neuroprotective effects of Huang-Lian-Jie-Du-Decoction on ischemic stroke rats revealed by (1)H NMR metabolomics approach.
Huang-Lian-Jie-Du-Decoction (HLJDD) is a representative antipyretic and detoxifying recipe in traditional Chinese medicine (TCM). This formula and its component herbs like Radix Scutellariae, Fructus Gardeniae show a variety of neuroprotective activities and have been used for the treatment of nervous system diseases including stroke. To comprehensively and holistically assess its therapeutic effect on ischemic stroke, a novel integrative metabolomics approach was applied. A rat ischemic stroke model was established by introduction of transient middle cerebral artery occlusion (MCAO) followed by reperfusion. The neurological deficit, cerebral infarct size and morphological abnormality were evaluated. An NMR technique combined with appropriate statistical analyses was then performed to explore the metabonomic profiles of serum and brain tissue extracts. Pattern analysis of the (1)H NMR data disclosed that HLJDD could relieve stroke rats suffering from the ischemia/reperfusion (I/R) injury by ameliorating the disturbance in energy metabolism, membrane and mitochondrial metabolism, neurotransmitter and amino acid metabolism, alleviating the oxidative stress from reactive oxygen species (ROS) and the inflammatory damage, and recovering the destructed osmoregulation.
State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China.
Peng-Ran
PR
Wang
Jun-Song
JS
Wang
Ming-Hua
MH
Yang
Ling-Yi
LY
Kong
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D020244:Infarction, Middle Cerebral Artery / Q000188:drug therapy; D007249:Inflammation; D007511:Ischemia / Q000188:drug therapy*; D016018:Least-Squares Analysis; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055432:Metabolomics; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D018377:Neurotransmitter Agents / Q000737:chemistry; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species; D015427:Reperfusion Injury; D020521:Stroke / Q000188:drug therapy*
D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D018377:Neurotransmitter Agents; D017382:Reactive Oxygen Species; C061676:oren gedoku to
Huang-Lian-Jie-Du-Decoction (HLJDD); Metabolomics; Middle cerebral artery occlusion (MCAO); Partial least squares discriminant analysis (PLS-DA); Proton nuclear magnetic resonance ((1)H NMR) spectroscopy