Genetically encoded fluorescent probe for the selective detection of peroxynitrite.
Peroxynitrite is a highly reactive molecule involved in cell signaling and pathological processes. We hereby report a novel genetically encoded probe, pnGFP, which can selectively sense peroxynitrite. A boronic acid moiety was site-specifically introduced into circularly permuted fluorescent proteins. By examining different protein templates followed with site-targeted random mutagenesis, we identified a selective peroxynitrite sensor, which is essentially unresponsive to other common cellular redox signaling molecules. The new probe has been genetically introduced into mammalian cells to image peroxynitrite at physiologically relevant concentrations.
Department of Chemistry, University of California , Riverside, California 92521, United States.
Zhi-jie
ZJ
Chen
Wei
W
Ren
Quintin E
QE
Wright
Hui-wang
HW
Ai
D015374:Biosensing Techniques / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D057054:Molecular Imaging; D016297:Mutagenesis, Site-Directed; D030421:Peroxynitrous Acid / Q000032:analysis*
D030421:Peroxynitrous Acid; D049452:Green Fluorescent Proteins