Therapeutic effect of Ginkgo biloba polysaccharide in rats with focal cerebral ischemia/reperfusion (I/R) injury.
An araban type polysaccharide (GBPw) was purified from the leaves of Ginkgo biloba. The present study aimed to investigate the protective effects of GBPw on focal ischemia/reperfusion (I/R) injury in rat brain. The results of this study demonstrated that GBPw had a positive effect on the rat brain when administered 7 days before focal cerebral I/R injury. This effect was evident with the improvements in neurological deficits, reduction in infarct volume, MDA content and the levels of pro-inflammatory cytokines (TNF-α and IL-1β), and elevation in the SOD and MPO activities and the levels of anti-inflammatory cytokine (IL-10). Thus, the beneficial effects of GBPw on cerebral I/R injury may result from the reduction of oxidative stress and the inhibition of NO production and inflammation induced by I/R. The neuroprotective effects of GBPw supplement may have potential implication in the future for prevention/protection against cerebral ischemic stroke.
Department of Neurology, the Second Affiliated Hospital, Harbin Medical University, Harbin 150081, China.
Yongmei
Y
Yang
Peifang
P
Liu
Lixia
L
Chen
Zhaojun
Z
Liu
Huixue
H
Zhang
Jianjian
J
Wang
Xuesong
X
Sun
Weiqi
W
Zhong
Na
N
Wang
Kuo
K
Tian
Jingshun
J
Zhao
D000818:Animals; D001921:Brain / Q000098:blood supply / Q000187:drug effects / Q000473:pathology; D001923:Brain Chemistry; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D020441:Ginkgo biloba / Q000737:chemistry*; D016753:Interleukin-10 / Q000819:agonists / Q000378:metabolism; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism
D053583:Interleukin-1beta; D018696:Neuroprotective Agents; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; C030080:araban; D016753:Interleukin-10; D009569:Nitric Oxide; D008315:Malondialdehyde; D009195:Peroxidase; D013482:Superoxide Dismutase
Cerebral ischemia/reperfusional; Ginkgo biloba L.; Neuroprotection; Polysaccharide; Purification