Tissue tropism of Ornithobacterium rhinotracheale in chickens determined by culture and nucleic acid detection.
The aim of the present study was to evaluate the ability of Ornithobacterium rhinotracheale (ORT) to colonize chosen organs of chicks infected intratracheally (group A1), or intravenously (group A2), with the use of bacteriological methods and PCR. The bacteriological methods enabled to reisolate ORT bacteria from trachea and lungs of the birds from group A1 only on day 3 and 6 after infection. The PCR technique additionally detected the bacterial genetic material in these organs on the 9th day after infection, and gave positive results in the samples from air sacs until the 6th day of the experiment. In birds infected intravenously (A2) ORT was reisolated from liver on day 3 and from spleen on day 3 and 6 after infection, whereas the reisolation from the tibiotarsal joint occurred during the entire experimental period. PCR enabled to detect the bacterial DNA in the liver, spleen and lungs of chickens until the 9th day after infection and in case of tibiotarsal joint during the whole time of the study.
Division of Avian Diseases, Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 159 Nowoursynowska Str., Warsaw 02-776, Poland. artur_zbikowski@sggw.pl
Artur
A
Zbikowski
Piotr
P
Szeleszczuk
Marian
M
Binek
D000818:Animals; D002645:Chickens; D004269:DNA, Bacterial / Q000235:genetics*; D045826:Flavobacteriaceae Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D006614:Hindlimb; D007596:Joints / Q000382:microbiology; D008099:Liver / Q000382:microbiology; D009928:Organ Specificity; D045246:Ornithobacterium / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D012137:Respiratory System / Q000382:microbiology; D013154:Spleen / Q000382:microbiology
D004269:DNA, Bacterial