The characterisation of pluripotent and multipotent stem cells using Fourier transform infrared microspectroscopy.
Fourier transform infrared (FTIR) microspectroscopy shows potential as a benign, objective and rapid tool to screen pluripotent and multipotent stem cells for clinical use. It offers a new experimental approach that provides a holistic measurement of macromolecular composition such that a signature representing the internal cellular phenotype is obtained. The use of this technique therefore contributes information that is complementary to that acquired by conventional genetic and immunohistochemical methods.
Department of Anatomy and Developmental Biology, Faculty of Medicine, Nursing and Health Sciences, Monash University, Wellington Road, Clayton, Victoria 3800, Australia. phil.heraud@monash.edu.
Julie
J
Cao
Elizabeth S
ES
Ng
Donald
D
McNaughton
Edouard G
EG
Stanley
Andrew G
AG
Elefanty
Mark J
MJ
Tobin
Philip
P
Heraud
D002454:Cell Differentiation; D016000:Cluster Analysis; D016002:Discriminant Analysis; D006801:Humans; D016018:Least-Squares Analysis; D039902:Multipotent Stem Cells / Q000737:chemistry* / Q000166:cytology; D039904:Pluripotent Stem Cells / Q000737:chemistry* / Q000166:cytology; D025341:Principal Component Analysis; D017550:Spectroscopy, Fourier Transform Infrared