Molecular characterization of photosensitizer-mediated photodynamic therapy by gene expression profiling.
Photodynamic therapy (PDT) is a novel cancer treatment based on the tumor-specific accumulation of a photosensitizer followed by irradiation with visible light, which induces selective tumor cell death via production of reactive oxygen species. To elucidate the underlying mechanisms, microarray analysis was used to analyze the changes in gene expression patterns during PDT induced by various photosensitizers. Cancer cells were subjected to four different photosensitizer-mediated PDT and the resulting gene expression profiles were compared. We identified many differentially expressed genes reported previously as well as new genes for which the functionfunctions in PDT are still unclear. Our current results not only advance the general understanding of PDT but also suggest that distinct molecular mechanisms are involved in different photosensitizer-mediated PDT. Elucidating the signaling mechanisms in PDT will provide information to modulate the antitumor effectiveness of PDT using various photosensitizers.
Department of Biotechnology, Chia Nan University of Pharmacy and Science, Tainan, Taiwan.
K-H
KH
Liu
Department of Otolaryngology, College of Medicine, National Taiwan University Hospital and National Taiwan University, Taipei, Taiwan.
C-P
CP
Wang
Biomedical Engineering Center, Industrial Technology Research Institute, Hsinchu, Taiwan.
M-F
MF
Chang
Biomedical Engineering Center, Industrial Technology Research Institute, Hsinchu, Taiwan.
Y-W
YW
Chung
Department of Otolaryngology, College of Medicine, National Taiwan University Hospital and National Taiwan University, Taipei, Taiwan.
P-J
PJ
Lou
Department of Biological Science and Technology, China Medical University, Taichung, Taiwan linjh@mail.cmu.edu.tw.
J-H
JH
Lin
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077195:Squamous Cell Carcinoma of Head and Neck; D013997:Time Factors
D017319:Photosensitizing Agents
Cancer biology; chemopreventation; drug toxicology