Systematic investigation of interactions between papain and MPA-capped CdTe quantum dots.
Fluorescent quantum dots (QDs) have been widely applied in biological and biomedical areas, but relatively little is known about the interaction of QDs with some natural enzymes. Herein, the interactions between 3-mercaptopropionic acid-capped CdTe QDs (MPA-QDs) and papain were systematically investigated by UV-Vis absorption spectra, fluorescence spectra and circular dichroism (CD) spectra under the physiological conditions. The fluorescence spectra results indicated that MPA-QDs quenched the fluorescence intensity of papain. The modified Stern-Volmer quenching constant K a at different temperatures and the corresponding thermodynamic parameters ΔH, ΔG and ΔS were also calculated. The binding of MPA-QDs and papain is a result of the formation of QDs-papain complex and the electrostatic interactions play a major role in stabilizing the complex. The CD technique was further used to analyze the conformational changes of papain induced by MPA-QDs and the results indicated that the biological activity of papain was affected by MPA-QDs dramatically.
College of Chemistry and Life Science, Guangxi Teachers Education University, Nanning, 530001, People's Republic of China.
Qi
Q
Xiao
Hangna
H
Qiu
Shan
S
Huang
Chusheng
C
Huang
Wei
W
Su
Baoqing
B
Hu
Yi
Y
Liu
D015097:3-Mercaptopropionic Acid / Q000737:chemistry*; D019187:Cadmium Compounds / Q000378:metabolism*; D002942:Circular Dichroism; D007700:Kinetics; D010206:Papain / Q000378:metabolism*; D011485:Protein Binding; D045663:Quantum Dots / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013691:Tellurium / Q000378:metabolism*; D013696:Temperature
D019187:Cadmium Compounds; D015097:3-Mercaptopropionic Acid; D010206:Papain; D013691:Tellurium; C028337:cadmium telluride