Endophilin, Lamellipodin, and Mena cooperate to regulate F-actin-dependent EGF-receptor endocytosis.
The epidermal growth factor receptor (EGFR) plays an essential role during development and diseases including cancer. Lamellipodin (Lpd) is known to control lamellipodia protrusion by regulating actin filament elongation via Ena/VASP proteins. However, it is unknown whether this mechanism supports endocytosis of the EGFR. Here, we have identified a novel role for Lpd and Mena in clathrin-mediated endocytosis (CME) of the EGFR. We have discovered that endogenous Lpd is in a complex with the EGFR and Lpd and Mena knockdown impairs EGFR endocytosis. Conversely, overexpressing Lpd substantially increases the EGFR uptake in an F-actin-dependent manner, suggesting that F-actin polymerization is limiting for EGFR uptake. Furthermore, we found that Lpd directly interacts with endophilin, a BAR domain containing protein implicated in vesicle fission. We identified a role for endophilin in EGFR endocytosis, which is mediated by Lpd. Consistently, Lpd localizes to clathrin-coated pits (CCPs) just before vesicle scission and regulates vesicle scission. Our findings suggest a novel mechanism in which Lpd mediates EGFR endocytosis via Mena downstream of endophilin.
King's College London, Randall Division of Cell and Molecular Biophysics, London, UK.
Anne
A
Vehlow
Daniel
D
Soong
Gema
G
Vizcay-Barrena
Cristian
C
Bodo
Ah-Lai
AL
Law
Upamali
U
Perera
Matthias
M
Krause
D008841:Actin Cytoskeleton / Q000378:metabolism / Q000502:physiology; D000199:Actins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002478:Cells, Cultured; D002966:Clathrin / Q000378:metabolism; D003034:Coated Pits, Cell-Membrane / Q000378:metabolism / Q000502:physiology; D004705:Endocytosis / Q000235:genetics*; D066246:ErbB Receptors / Q000378:metabolism*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D041681:NIH 3T3 Cells; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology
D000199:Actins; D002352:Carrier Proteins; D002966:Clathrin; C511939:Enah protein, human; D008565:Membrane Proteins; D008840:Microfilament Proteins; C493339:RAPH1 protein, human; D000217:Acyltransferases; C018764:2-acylglycerophosphate acyltransferase; D066246:ErbB Receptors