Live imaging of Arabidopsis development.
Live cell imaging is an essential methodology for studying the structure, dynamics, and functions of cells in a living plant under normal or stressed growth conditions. Arabidopsis thaliana is perfectly amenable to various live microscopy techniques. In this chapter, we provide guidelines to design live-imaging experiments. We discuss specifically the respective advantage of each microscopy technique, the choice of reporter, and the preparation of the sample. Detailed protocols for imaging of shoot and roots are provided.
Physical Biology, Frankfurt Institute for Molecular Life Sciences (FMLS), Goethe Universität Frankfurt am Main, Frankfurt am Main, Germany.
Daniel
D
von Wangenheim
Gabor
G
Daum
Jan U
JU
Lohmann
Ernst K
EK
Stelzer
Alexis
A
Maizel
D017360:Arabidopsis / Q000166:cytology / Q000254:growth & development*; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008856:Microscopy, Fluorescence; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development*; D018520:Plant Shoots / Q000166:cytology / Q000254:growth & development*; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D012639:Seeds / Q000254:growth & development
D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins