Molecular characterization, serotyping, and antibiotic susceptibility profile of Leptospira interrogans serovar Copenhageni isolates from Brazil.
Leptospira interrogans serogroup Icterohaemorrhagiae is the major serogroup infecting humans worldwide, and rodents and dogs are the most significant transmission sources in urban environments. Knowledge of the prevalent serovars and their maintenance hosts is essential to understand the epidemiology of leptospirosis. In this study, 20 Leptospira isolates were evaluated by pulsed-field gel electrophoresis (PFGE), variable number tandem-repeat analysis (VNTR), serotyping, and determination of antimicrobial resistance profile. Isolates, originated from bovine, canine, human, and rodent sources, were characterized by microscopic agglutination test with polyclonal and monoclonal antibodies and were identified as L. interrogans serogroup Icterohaemorrhagiae serovar Copenhageni. MICs of antimicrobials often used in veterinary medicine were determined by broth microdilution test. Most of tested antibiotics were effective against isolates, including penicillin, ampicillin, and ceftiofur. Higher MIC variability was observed for fluoroquinolones and neomycin; all isolates were resistant to trimethoprim/sulfamethoxazole and sulphadimethoxine. Isolates were genotyped by PFGE and VNTR; both techniques were unable to discriminate between serovars Copenhageni and Icterohaemorrhagiae, as expected. PFGE clustered all isolates in 1 pulsotype, indicating that these serovars can be transmitted between species and that bovine, rodent, and dogs can maintain them in the environment endangering the human population.
Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo - SP 05508-270, Brazil.
Fabiana
F
Miraglia
Minekazo
M
Matsuo
Zenaide Maria
ZM
Morais
Odir Antonio
OA
Dellagostin
Fabiana Kömmling
FK
Seixas
Julio César
JC
Freitas
Rudy
R
Hartskeerl
Luisa Zanolli
LZ
Moreno
Bárbara Letícia
BL
Costa
Gisele Oliveira
GO
Souza
Silvio Arruda
SA
Vasconcellos
Andrea Micke
AM
Moreno
D000372:Agglutination Tests; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001938:Brazil; D002417:Cattle; D016000:Cluster Analysis; D004285:Dogs; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D007921:Leptospira interrogans / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D007922:Leptospirosis / Q000382:microbiology* / Q000662:veterinary*; D008826:Microbial Sensitivity Tests; D018598:Minisatellite Repeats; D058889:Molecular Typing; D010641:Phenotype; D012377:Rodentia; D012703:Serotyping
D000900:Anti-Bacterial Agents
Animals; Copenhageni; Genotyping; Human; Leptospirosis; MIC