First report of circulating microRNAs in tumour necrosis factor receptor-associated periodic syndrome (TRAPS).
Tumor necrosis factor-receptor associated periodic syndrome (TRAPS) is a rare autosomal dominant autoinflammatory disorder characterized by recurrent episodes of long-lasting fever and inflammation in different regions of the body, such as the musculo-skeletal system, skin, gastrointestinal tract, serosal membranes and eye. Our aims were to evaluate circulating microRNAs (miRNAs) levels in patients with TRAPS, in comparison to controls without inflammatory diseases, and to correlate their levels with parameters of disease activity and/or disease severity. Expression levels of circulating miRNAs were measured by Agilent microarrays in 29 serum samples from 15 TRAPS patients carrying mutations known to be associated with high disease penetrance and from 8 controls without inflammatory diseases. Differentially expressed and clinically relevant miRNAs were detected using GeneSpring GX software. We identified a 6 miRNAs signature able to discriminate TRAPS from controls. Moreover, 4 miRNAs were differentially expressed between patients treated with the interleukin (IL)-1 receptor antagonist, anakinra, and untreated patients. Of these, miR-92a-3p and miR-150-3p expression was found to be significantly reduced in untreated patients, while their expression levels were similar to controls in samples obtained during anakinra treatment. MiR-92b levels were inversely correlated with the number of fever attacks/year during the 1(st) year from the index attack of TRAPS, while miR-377-5p levels were positively correlated with serum amyloid A (SAA) circulating levels. Our data suggest that serum miRNA levels show a baseline pattern in TRAPS, and may serve as potential markers of response to therapeutic intervention.
Research Center of Systemic Autoimmune and Autoinflammatory Diseases, Rheumatology Unit, Policlinico Le Scotte, University of Siena, Siena, Italy.
Orso Maria
OM
Lucherini
Laura
L
Obici
Manuela
M
Ferracin
Valerio
V
Fulci
Michael F
MF
McDermott
Giampaolo
G
Merlini
Isabella
I
Muscari
Flora
F
Magnotti
Laura J
LJ
Dickie
Mauro
M
Galeazzi
Massimo
M
Negrini
Cosima Tatiana
CT
Baldari
Rolando
R
Cimaz
Luca
L
Cantarini
D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005334:Fever; D056660:Hereditary Autoinflammatory Diseases / Q000188:drug therapy / Q000235:genetics*; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000627:therapeutic use; D008297:Male; D035683:MicroRNAs; D008875:Middle Aged; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000235:genetics
D053590:Interleukin 1 Receptor Antagonist Protein; D035683:MicroRNAs; D047888:Receptors, Tumor Necrosis Factor, Type I