Cell dispersion culture for the effective growth of Humicola insolens and efficient enzyme production.
We have developed a method for the effective growth of Humicola insolens conducive to efficient enzyme production using a medium containing glucose as a carbon source and extruded soybean meal (ExSBM) as a nitrogen source. Enzymes from Humicola sp. hold promise for biomass degradation, especially of lignocellulosic materials such as rice straw, wood chips, and corn stover. The strain, however, is hard to disperse, so an aggregated form of the fungus in a liquid culture media is generally used, resulting in poor control of the growth process and low enzyme production. This has greatly limited the utilization of this strain, in spite of its potential as an enzyme producer. Surprisingly, the addition of ExSBM improves mycelium dispersion and enzyme production of H. insolens, and the dispersive effect is applicable to other fungi such as Trichoderma and Aspergillus sp. In contrast, defatted soybean meal not treated with an extrusion process has little effect on mycelium cohesion. It therefore appears that the specific three-dimensional structure of ExSBM arising from the extrusion process provides a favorable environment for cell growth, since the composition of ExSBM and soybean meal is essentially identical. The optimum medium for cell dispersion culture essentially consisted of 5% glucose and 0.3% ExSBM.
Enzymology and Molecular Biology Laboratory, Department of Chemistry and Life Science, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
Hitoshi
H
Matsumoto
Enzymology and Molecular Biology Laboratory, Department of Chemistry and Life Science, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
Keisuke
K
Koganei
Enzymology and Molecular Biology Laboratory, Department of Chemistry and Life Science, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
Naoko
N
Nishida
Biomolecular Engineering Laboratory, National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Yoshiyuki
Y
Koyama
Oilseed Protein Laboratory, J-Oil Mills, Inc., 7-41 Daikoku-cho, Tsurumi-ku, Yokohama 230-0053, Japan.
Sanshiro
S
Saito
Oilseed Protein Laboratory, J-Oil Mills, Inc., 7-41 Daikoku-cho, Tsurumi-ku, Yokohama 230-0053, Japan.
Hisashi
H
Kataoka
Enzymology and Molecular Biology Laboratory, Department of Chemistry and Life Science, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
Jun
J
Ogihara
Enzymology and Molecular Biology Laboratory, Department of Chemistry and Life Science, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan. Electronic address: kasumi@brs.nihon-u.ac.jp.
Takafumi
T
Kasumi
D001203:Ascomycota / Q000201:enzymology* / Q000254:growth & development* / Q000648:ultrastructure; D001673:Biodegradation, Environmental; D018533:Biomass; D018929:Cell Culture Techniques; D002480:Cellulase / Q000096:biosynthesis; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D012275:Oryza / Q000378:metabolism*; D013025:Glycine max / Q000737:chemistry; D014242:Trichoderma / Q000201:enzymology; D014995:Xylosidases / Q000096:biosynthesis
D003470:Culture Media; D014995:Xylosidases; D002480:Cellulase; C019826:carboxymethylcellulase
Cell dispersion culture; Cellulase; Extruded soybean meal; Hemicellulase; Humicola insolens; Soybean meal