[Study on effect of weinaokang and bilobalide on autophagy and neurogenesis induced by focal cerebral ischemia reperfusion].
OBJECTIVE
To observe the protective effect of the Weinaokang (WNK) and its active compound bilobalide on focal cerebral ischemia reperfusion, and their mechanisms.


METHODS
The 60-minute middle cerebral artery occlusion (MCAO) was adopted to establish the 24 h-14 d reperfusion model. The expression of Beclin-1 was detected by the Western blotting technique. The transmission electron microscopy was used to observe ultrastructural changes. Neurogenesis was detected by the immunofluorescence staining.


RESULTS
WNK (20, 10 mg x kg(-1), ig) or its active compound bilobalide (10, 5 mg x kg(-1), ig) could promote the generation of mature neurons (BrdU(+) -MAP-2+) at the ischemic side, and inhibit expression of autophagy-related gene Beclin-1, so as to reduce the neuron injury induced by focal cerebral ischemia reperfusion.


CONCLUSIONS
WNK and its active compound bilobalide can inhibit neuron autophagy and improve neurogenesis in ischemic peripheral area, suggesting that neurogenesis may be the intervention target for WNK to promote self-repairing of ischemic area.
Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing 100091, China.
Yong-Qiu
YQ
Zheng
Jian-Xun
JX
Liu
Li
L
Xu
Ming-Jiang
MJ
Yao
Wen-Ting
WT
Song
D000818:Animals; D001343:Autophagy / Q000187:drug effects*; D002545:Brain Ischemia / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D003517:Cyclopentanes / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005663:Furans / Q000494:pharmacology*; D046934:Ginkgolides / Q000494:pharmacology*; D008297:Male; D055495:Neurogenesis / Q000187:drug effects*; D009474:Neurons / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*
D003517:Cyclopentanes; D004365:Drugs, Chinese Herbal; D005663:Furans; D046934:Ginkgolides; C552039:weinaokang; C073710:bilobalide