[Effect of hydroxyapatite nanoparticles on MII-stage porcine oocytes vitrification and the study of its mechanism].
Nano-cryopreservation may become a new way in the next generation of cryopreservation technology. However, research using nanoparticles in oocytes vitrification has not been reported in the literature. In this study, HA nanoparticles with different diameters were added into cryoprotectant and M II-stage porcine oocytes were vitrified by Cryotop. The results showed that nanoparticles improved the survival rate of cryopreserved M II-stage porcine oocytes, but the difference between nanoparticles with different diameters of was not significant. In order to study the mechanism of nano-cryopreservation, the cooling rate of cryoprotectant was measured by ultra-fast temperature measurement system and the melting enthalpy of cryoprotectant was measured by differential scanning calorimeter (DSC). The results showed that the adding of nanoparitcles could not increase the cooling rate of cryoprotectant, but could decreases the amount of ice crystals during freezing and warming. Therefore, the mechanical injury within and outside cells might be effectively reduced.
Institute of Biothermal Technology, University of Shanghai for Science and Technology, Shanghai 200093, China.
Weijie
W
Li
Xinli
X
Zhou
Jiangjun
J
Dai
Defu
D
Zhang
Baolin
B
Liu
Haisong
H
Wang
Li
L
Xu
D000818:Animals; D002470:Cell Survival / Q000502:physiology; D015925:Cryopreservation / Q000379:methods* / Q000662:veterinary; D003451:Cryoprotective Agents / Q000494:pharmacology; D017886:Durapatite / Q000494:pharmacology*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary; D008677:Metaphase; D053758:Nanoparticles; D009865:Oocytes / Q000166:cytology*; D013552:Swine; D058989:Vitrification
D003451:Cryoprotective Agents; D017886:Durapatite