Interaction peculiarities of 5,10,15,20-tetrakis(4-N-methylpyridil) tetra iodide porphyrin with albumin.
In present work interactions of bovine serum albumin with 5,10,15,20-tetrakis(4-N-methylpyridil) tetra iodide porphyrin have been studied by electron absorption and fluorescence spectroscopy. The studies were carried out in aqueous media at different pH and in water-dimethylformamide mixtures containing up to 0.19 M of the organic solvent. It has been demonstrated that the porphyrin forms stable complexes with BSA in which the porphyrin is located subdomains IB and IIA. The stability constants of the complexes is practically independent of pH.
G.A. Krestov Institute of Solution Chemistry of RAS, Ivanovo, Russian Federation.
N Sh
NSh
Lebedeva
E A
EA
Malkova
T E
TE
Popova
A E
AE
Kutyrev
S A
SA
Syrbu
E V
EV
Parfenyuk
A I
AI
Vyugin
D000818:Animals; D002417:Cattle; D007454:Iodides / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D011166:Porphyrins / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D007454:Iodides; D011166:Porphyrins; D012710:Serum Albumin, Bovine
Bovine serum albumin; Electronic absorption; Fluorescence spectra; Polymer complex; Porphyrins