Simultaneous analysis of eight phenolic environmental estrogens in blood using dispersive micro-solid-phase extraction combined with ultra fast liquid chromatography-tandem mass spectrometry.
A novel, simple and sensitive method was developed for the simultaneous determination of eight phenolic environmental estrogens in blood by using the dispersive micro-solid-phase extraction (d-µ-SPE) procedure combined with ultra-fast liquid chromatography-tandem quadrupole mass spectrometry (UFLC-MS/MS). The excellent nanomaterials tetraethylenepentamine-functionalized magnetic polymer was used as an adsorbent, and the main factors affecting the extraction efficiency were investigated in detail. All target compounds showed good linearities in the tested range with correlation coefficients (r) higher than 0.999. The mean recoveries were in the range of 85.0-105.0%. The intra-day and inter-day relative standard deviations (RSDs) were lower than 4.9% and 5.2%, respectively. The limits of quantification for the eight phenolic environmental estrogens were between 0.075 and 0.42 µg L(-1). The developed method can be applied to the routine analyses for the determination of the eight phenolic environmental estrogens in blood samples.
Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China.
Yong-Gang
YG
Zhao
Xiao-Hong
XH
Chen
Sheng-Dong
SD
Pan
Hao
H
Zhu
Hao-Yu
HY
Shen
Mi-Cong
MC
Jin
D000327:Adsorption; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D052244:Endocrine Disruptors / Q000097:blood*; D004785:Environmental Pollutants / Q000097:blood*; D004967:Estrogens / Q000097:blood*; D005029:Ethylenediamines / Q000737:chemistry*; D057230:Limit of Detection; D059346:Magnets; D049329:Nanostructures / Q000737:chemistry*; D010636:Phenols / Q000097:blood*; D058105:Polymerization; D011892:Rana catesbeiana; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods; D053719:Tandem Mass Spectrometry
D052244:Endocrine Disruptors; D004785:Environmental Pollutants; D004967:Estrogens; D005029:Ethylenediamines; D010636:Phenols; C034269:tetraethylenepentamine
Blood; Dispersive micro-solid-phase extraction (d-µ-SPE); Environmental estrogens; Multiple reaction monitoring (MRM); Tetraethylenepentamine (TEPA)-functionalized magnetic polymers (TEPA-MPs); Ultra fast liquid chromatography–tandem mass spectrometry (UFLC–MS/MS)