Expression of programmed death-1 (PD-1) on CD4+ and CD8+ T cells in rheumatoid arthritis.
Rheumatoid arthritis (RA) is characterized by chronic inflammatory process that targets the synovial lining of diarthrodial joints. Programmed death 1 (PD-1) plays a key role in the negative regulation of the immune response. In the current study, we investigated the expression of PD-1 on peripheral CD4+ and CD8+ T cells in RA patients. Percentage of PD-1+ cells was measured by flow cytometry in 82 RA cases and 90 healthy controls. Results showed that PD-1 expression was significantly decreased in both peripheral CD4+ and CD8+ T cells in RA (p = 0.002 and p < 0.001, respectively). Similarly, serum levels of soluble PD-1 were also downregulated in RA cases. When comparing PD-1 level in RA patients with different clinical parameters, patients with positive C-reactive protein (CRP) revealed lower proportion of PD-1 on CD4+ and CD8+ T cells than those with negative CRP. Also, disease activity score of RA patients was inversely correlated with PD-1 expression on peripheral CD4+ and CD8+ T cells. These data suggested that PD-1 may act as a negative regulator in the pathogenesis and progression of RA.
Department of Orthopedics, Shandong Qianfoshan Hospital, 16766 Jing Shi Road, Jinan, Shandong, 250014, China.
Shufeng
S
Li
Wensheng
W
Liao
Meng
M
Chen
Shiying
S
Shan
Yuanlin
Y
Song
Shuzhen
S
Zhang
Haihan
H
Song
Zhen
Z
Yuan
D000328:Adult; D001172:Arthritis, Rheumatoid / Q000276:immunology / Q000473:pathology*; D002097:C-Reactive Protein / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D015536:Down-Regulation; D005260:Female; D006801:Humans; D007249:Inflammation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D061026:Programmed Cell Death 1 Receptor / Q000096:biosynthesis* / Q000097:blood*; D013583:Synovial Membrane / Q000473:pathology
C105992:PDCD1 protein, human; D061026:Programmed Cell Death 1 Receptor; D002097:C-Reactive Protein