Identification of proteins interacting with HSP70 mRNAs in Leishmania braziliensis.
HSP70 protein is involved in Leishmania differentiation, apoptosis, antimony-resistance and host-immune response. Therefore, this protein and the regulatory mechanisms of HSP70 gene expression are promising targets for therapeutic intervention against leishmaniasis. The regulation of mRNA expression in trypanosomatids operates mostly through the interaction of trans-acting proteins, and elements located in the untranslated regions of mRNAs. The aim of this work was to identify protein factors interacting specifically with the Leishmania braziliensis HSP70 mRNAs. Thus, the 5' UTR and the two types of 3' UTRs (UTR-I and UTR-II) from L. braziliensis HSP70 genes were used as baits in pull down assays using total protein extracts from parasites cultured at 26 or 35°C. The captured proteins were resolved by two-dimensional gel electrophoresis (2-DE) and identified by mass spectrometry (MS) analysis. As a result, 52 different proteins were identified based on their binding to the L. braziliensis HSP70-mRNAs. As expected, several of the identified proteins were related to RNA metabolism (27%) and translation process (7%). In addition, five hypothetical conserved proteins having motifs related with RNA interaction were also identified (9.6%). Nevertheless, unexpected proteins, apparently unrelated to the mRNA expression, were also identified. The biological significance of these and others L. braziliensis detected proteins, including the HSP70 itself, is discussed.


CONCLUSIONS
For the first time, a riboproteomic analysis of the proteins interacting with the untranslated regions of the heat shock protein 70 (HSP70) mRNA from Leishmania braziliensis was carried out. This work provides new insights related to protein factors putatively involved in the regulation of HSP70 gene expression in L. braziliensis, and thereby, contributes to a better understanding of the parasite biology, and ultimately to the development of novel therapeutic interventions for controlling the important diseases caused by this parasite.
Laboratorio de Parasitología Molecular, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Carrera 7 No. 43-82, Edificio 52, Oficina 608, Bogotá, Colombia; Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM), Universidad Autónoma de Madrid, 28049 Madrid, Spain. Electronic address: cesar-ramirez@javeriana.edu.co.
C A
CA
Ramírez
M A
MA
Dea-Ayuela
M D
MD
Gutiérrez-Blázquez
F
F
Bolas-Fernández
J M
JM
Requena
C J
CJ
Puerta
D020413:3' Untranslated Regions / Q000502:physiology*; D020121:5' Untranslated Regions / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D007892:Leishmania braziliensis / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins / Q000096:biosynthesis* / Q000235:genetics; D016053:RNA, Protozoan / Q000235:genetics / Q000378:metabolism*
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D018840:HSP70 Heat-Shock Proteins; D015800:Protozoan Proteins; D016053:RNA, Protozoan
HSP70 UTRs; HSP70 protein; Leishmania braziliensis; Mass spectrometry; Pull-down; mRNA expression regulation