A new, simple, green, and one-pot four-component synthesis of bare and poly(α,γ, L-glutamic acid)-capped silver nanoparticles.
A simple and green chemical method has been developed to synthesize stable bare and capped silver nanoparticles based on the reduction of silver ions by glucose and capping by poly(α,γ,L-glutamic acid) (PGA). The use of ammonia during synthesis was avoided. PGA has had a dual role in the synthesis and was used as a capping agent to make the silver nanoparticle more biocompatible and to protect the nanoparticles from agglomerating in the liquid medium. The synthesized PGA-capped silver nanoparticles in the size range 5-45 nm were stable over long periods of time, without signs of precipitation. Morphological examination has shown that the silver nanoparticles had a nearly spherical, multiply twinned structure. The effects of the reaction temperature and the reaction time during the synthesis were investigated too. The biocompatibility of the PGA-capped silver nano-particles is discussed in terms of in vitro toxicity with human intestinal Caco-2 cells. The samples were characterized by UV-Visible spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, and zeta potential measurements.
Institute of Technical Sciences of the Serbian Academy of Sciences and Arts, Knez Mihailova 35/IV, Belgrade 11000, Serbia.
Magdalena
M
Stevanović
Igor
I
Savanović
Vuk
V
Uskoković
Srečo D
SD
Skapin
Ines
I
Bračko
Uroš
U
Jovanović
Dragan
D
Uskoković
Capping agent; Green method; In vitro toxicity; Poly(α,γ,L-glutamic acid); Silver nanoparticles