Beneficial effects of a polysaccharide from Salvia miltiorrhiza on myocardial ischemia-reperfusion injury in rats.
In the present study, one water-soluble polysaccharide (SMP1) was isolated from the roots of Salvia miltiorrhiza. The cardio-protective potential of SMP1 was studied in the ischemia-reperfusion (I/R) model of rats in vivo. Results showed that 30 min of left anterior descending coronary artery occlusion (LAD) followed by 4 h of reperfusion markedly decreased myocardial superoxide dismutase (SOD), Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase activities and increased myocardial malondialdehyde (MDA) level and serum activities of creatine kinase (CK) and lactate dehydrogenase (LDH) in I/R rats. An increase in infarct size and high apoptosis index of cardiac cell were also observed in IR rats. Administration of SMP1 400 and 800 mg/kg significantly reversed these biochemical parameters in the I/R rats to the normal levels in sham control rats. The infarct sizes and the percent of TUNEL-positive cells were found significantly decreased in SMP1-treated groups compared to I/R rats. Taken together, the present study clearly suggests SMP1 has a protective effect against myocardial I/R injury in rats by ameliorating oxidative stress and inhibiting myocardial apoptosis.
Department of Cardiology, The Second Affiliated Hospital, Third Military Medical University, Xinzheng Street No. 183, Shapingba District, Chongqing 400037, China.
Mingbao
M
Song
Lan
L
Huang
Gang
G
Zhao
Yaoming
Y
Song
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002316:Cardiotonic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D003402:Creatine Kinase / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006321:Heart / Q000187:drug effects* / Q000503:physiopathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000473:pathology; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D012995:Solubility; D013482:Superoxide Dismutase / Q000378:metabolism; D014867:Water
D002316:Cardiotonic Agents; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D014867:Water; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; D003402:Creatine Kinase; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase
Anti-apoptotic; Antioxidative; Myocardial ischemia–reperfusion injury; Polysaccharides; Salvia miltiorrhiza