Proteomic analysis of leaves and roots of common wheat (Triticum aestivum L.) under copper-stress conditions.
Proteomic studies were performed to identify the protein species involved in copper (Cu) stress responses in common wheat. Two-week-old wheat seedlings were exposed to 100 μM CuSO4 treatment for 3 days. Growth of shoots and roots was markedly inhibited and lipid peroxidation was greatly increased. Cu was readily absorbed by wheat seedlings, with greater Cu contents in roots than in leaves. Using 2-DE method, 98 protein spots showed significantly enhanced or reduced abundance, of which 93 were successfully identified. Of these identified protein species, 49 and 44 were found in roots and leaves, respectively. Abundance of most of identified protein species, which function in signal transduction, stress defense, and energy production, was significantly enhanced, while that of many protein species involved in carbohydrate metabolism, protein metabolism, and photosynthesis was severely reduced. The Cu-responsive protein interaction network revealed 36 key proteins, most of which may be regulated by abscisic acid (ABA), ethylene, jasmonic acid (JA), and so on. Exogenous JA application showed a protective effect against Cu stress and significantly increased transcripts of the glutathione S-transferase (GST) gene. This study provides insight into the molecular mechanisms of Cu responses in higher plants.
The Synergetic Innovation Center of Henan Grain Crops, Henan Agricultural University , Zhengzhou 450002, China.
Gezi
G
Li
Xiaoqi
X
Peng
Hongmei
H
Xuan
Liting
L
Wei
Yingying
Y
Yang
Tiancai
T
Guo
Guozhang
G
Kang
D000704:Analysis of Variance; D019327:Copper Sulfate / Q000493:pharmacokinetics / Q000633:toxicity*; D003517:Cyclopentanes / Q000494:pharmacology; D017931:DNA Primers / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant / Q000187:drug effects* / Q000235:genetics; D005982:Glutathione Transferase / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D015227:Lipid Peroxidation / Q000187:drug effects; D054883:Oxylipins / Q000494:pharmacology; D018515:Plant Leaves / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics; D013312:Stress, Physiological / Q000235:genetics*; D053719:Tandem Mass Spectrometry; D014908:Triticum / Q000235:genetics* / Q000378:metabolism
D003517:Cyclopentanes; D017931:DNA Primers; D054883:Oxylipins; D010940:Plant Proteins; C011006:jasmonic acid; D005982:Glutathione Transferase; D019327:Copper Sulfate