Structural and functional characterization of annexin 1 from Medicago truncatula.
Annexins are calcium- and membrane-binding proteins that have been shown to have diverse properties such as actin, integrin and GTP binding, both in animals and plants. Recently, Medicago truncatula annexin 1 (AnnMt1) has been suggested to participate in nodulation (Nod factor signaling) and mycorrhization in legume plants. In this report we demonstrate for the first time that recombinant AnnMt1 (rec-AnnMt1) mediates membrane permeabilization to cations with conductance ranging from 16 pS to 329 pS. In agreement with other structurally determined annexins, homology modeling of AnnMt1 suggests that most of the functional determinants are found on the convex surface of the modeled structure. In conclusion, we propose a potential constitutive role of AnnMt1 in Nod factor signaling as a non-specific ion channel.
Department of Biochemistry, Nencki Institute of Experimental Biology, 3 Pasteur Street, PL-02093 Warsaw, Poland.
Praveen Kumar
PK
Kodavali
Krzysztof
K
Skowronek
Izabela
I
Koszela-Piotrowska
Agnieszka
A
Strzelecka-Kiliszek
Krzysztof
K
Pawlowski
Slawomir
S
Pikula
D017302:Annexins / Q000737:chemistry / Q000378:metabolism*; D002412:Cations / Q000378:metabolism; D002463:Cell Membrane Permeability; D007473:Ion Channels / Q000737:chemistry / Q000378:metabolism*; D046913:Medicago truncatula / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology; D008954:Models, Biological; D015394:Molecular Structure; D038821:Mycorrhizae; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D055170:Plant Root Nodulation; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000378:metabolism; D015398:Signal Transduction
D017302:Annexins; D002412:Cations; D007473:Ion Channels; D010940:Plant Proteins; D011994:Recombinant Proteins
3-D; AnnAt1–AnnAt8; AnnMt1–AnnMt9; Annexin 1; AnxA1–A11 and AnxA13; CVs; Ion channel; Medicago truncatula; PMSF; Protein structure; annexins 1–8 from Arabidopsis thaliana; annexins 1–9 from Medicago truncatula; column volumes; mammalian annexins A1–A11 and A13; phenyl methyl sulfonyl fluoride; three-dimensional