Allelic diversity and molecular characterization of puroindoline genes in five diploid species of the Aegilops genus.
Grain hardness is an important quality trait in wheat. This trait is related to the variation in, and the presence of, puroindolines (PINA and PINB). This variation can be increased by the allelic polymorphism present in the Aegilops species that are related to wheat. This study evaluated allelic Pina and Pinb gene variability in five diploid species of the Aegilops genus, along with the molecular characterization of the main allelic variants found in each species. This polymorphism resulted in 16 alleles for the Pina gene and 24 alleles for the Pinb gene, of which 10 and 17, respectively, were novel. Diverse mutations were detected in the deduced mature proteins of these alleles, which could influence the hardness characteristics of these proteins. This study shows that the diploid species of the Aegilops genus could be a good source of genetic variability for both Pina and Pinb genes, which could be used in breeding programmes to extend the range of different textures in wheat.
Departamento de Genética, Escuela Técnica Superior de Ingeniería Agronómica y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, CeiA3, ES-14071 Córdoba, Spain.
Susana
S
Cuesta
Carlos
C
Guzmán
Juan B
JB
Alvarez
D000483:Alleles; D000595:Amino Acid Sequence; D004171:Diploidy; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006109:Poaceae / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment
D010940:Plant Proteins
Aegilops sp.; diversity; grain hardness; non-AUG start codon; puroindolines; wheat.