Protection against Vibrio alginolyticus in crimson snapper Lutjanus erythropterus immunized with a DNA vaccine containing the ompW gene.
The outer membrane proteins of Vibrio alginolyticus play an important role in the virulence of the bacterium and are potential candidates for vaccine development. In the present study, the ompW gene was cloned, expressed and purified. A DNA vaccine was constructed by inserting the ompW gene into a pcDNA plasmid. Crimson snapper Lutjanus erythropterus (Bloch) were injected intramuscularly with the recombinant plasmid pcDNA-ompW. The expression of the DNA vaccine was detected in gill, head kidney, heart, liver, spleen and injection site muscle of crimson snapper by RT-PCR 7 and 28 d post-vaccination. The ELISA results demonstrated that the DNA vaccine produced an observable antibody response in all sera of the vaccinated fish. In addition, crimson snapper immunized with the DNA vaccine showed a relative percentage survival (RPS) of 92.53%, indicating effective protection against V. alginolyticus infection.
Fisheries College, Guangdong Ocean University, Zhanjiang 524088, PR China.
Shuang-Hu
SH
Cai
Yi-Shan
YS
Lu
Ji-Chang
JC
Jian
Bei
B
Wang
Yu-Cong
YC
Huang
Ju-Fen
JF
Tang
Yu
Y
Ding
Zao-He
ZH
Wu
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology* / Q000378:metabolism; D001428:Bacterial Vaccines / Q000276:immunology*; D005393:Fish Diseases / Q000382:microbiology / Q000517:prevention & control*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D010473:Perciformes; D019444:Vaccines, DNA / Q000276:immunology; D014735:Vibrio Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D044149:Vibrio alginolyticus / Q000276:immunology* / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D019444:Vaccines, DNA