Point-of-care diagnosis and prognostication of cryptococcal meningitis with the cryptococcal antigen lateral flow assay on cerebrospinal fluid.
The cryptococcal antigen (CRAG) lateral flow assay (LFA) had 100% sensitivity and specificity on cerebrospinal fluid samples. Pretreatment LFA titers correlated with quantitative cultures (R(2) = 0.7) and predicted 2- and 10-week mortality. The CRAG LFA is an accurate diagnostic assay for CSF and should be considered for point-of-care diagnosis of cryptococcal meningitis.
Department of Medicine, Faculty of Medicine, Mbarara University of Science and Technology, Uganda.
Taseera
T
Kabanda
Mark J
MJ
Siedner
Jeffrey D
JD
Klausner
Conrad
C
Muzoora
David R
DR
Boulware
D002555:Cerebrospinal Fluid / Q000382:microbiology*; D002846:Chromatography, Affinity / Q000379:methods*; D015331:Cohort Studies; D003454:Cryptococcus / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D016919:Meningitis, Cryptococcal / Q000175:diagnosis*; D019095:Point-of-Care Systems; D012680:Sensitivity and Specificity
HIV; cryptococcal meningitis; diagnosis; point-of-care; sub-Saharan Africa