UGT1A1 promoter polymorphism associated with serum bilirubin level in Saudi patients with sickle cell disease.
OBJECTIVE
Polymorphism in (TA)n of the UGT1A1 promoter influences bilirubin level and risk of gallstones in patients with sickle cell disease (SCD) of African descent. Modifiers of bilirubin level and gallstones in Saudi patients with SCD are not known.


METHODS
Patients with SCD presenting to participating institutions between July 2009 and July 2012 were enrolled in our study.


METHODS
A total of 223 SCD patients were enrolled. Laboratory workup at steady state included complete blood count, reticulocytes, serum bilirubin, lactate dehydrogenase (LDH), G6PD level, and hemoglobin (Hb) electrophoresis. The (TA)n UGT1A1 promoter polymorphism and presence of a-thalassemia were also deter.mined.


RESULTS
TA6/6 in the UGT1A1 promoter was identified in 189 patients (84.7%), TA7/7 in 26 (11.7%), TA5/5 in 6 (2.7%), and TA5/6 in 2 (0.9%). Increased (TA)n of the UGT1A1 promoter (P < .0001), male gender (P=.02), higher LDH (P=.001), and lower Hb level (P=.009) were associated with higher bilirubin level, while the co-inheritance of a-thalassemia (P=.003) was linked with lower bilirubin level. UGT1A1 (TA)n (P < .0001) and Hb level (P=.005) remained significant on multivariate analysis. Gallstones were more frequent in patients with TA7/7 (72%) compared to patients with TA6/6 (57%) and TA5/5 or 5/6 (37%); however, this difference was not statistically significance (P=.18). Older age (P=.0001) and absence of a-thalassemia (P=.03) were associated with higher risk of gallstones.


CONCLUSIONS
(TA)n in the UGT1A1 promoter and intensity of hemolysis modify steady-state serum bilirubin level in SCD. Co-inheritance of a-thalassemia reduces the risk of gallstones in Saudi patients with SCD.
Zainab
Z
Hamad
Abdullah
A
Aljedai
Rabih
R
Halwani
Abdulrahman
A
AlSultan
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000755:Anemia, Sickle Cell / Q000150:complications / Q000235:genetics*; D001663:Bilirubin / Q000097:blood*; D044383:Black People; D002648:Child; D005260:Female; D042882:Gallstones / Q000453:epidemiology* / Q000235:genetics / Q000473:pathology; D014453:Glucuronosyltransferase / Q000235:genetics*; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D008297:Male; D015999:Multivariate Analysis; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D012307:Risk Factors; D012529:Saudi Arabia / Q000453:epidemiology; D012737:Sex Factors; D055815:Young Adult; D017085:alpha-Thalassemia / Q000453:epidemiology*; D000099148:UGT1A1 Enzyme
D001663:Bilirubin; D014453:Glucuronosyltransferase; D006454:Hemoglobins; D000099148:UGT1A1 Enzyme