Characterization of multi-drug resistant Mycobacterium tuberculosis from immigrants residing in the USA using Ion Torrent full-gene sequencing.
Drug-resistant Mycobacterium tuberculosis bacterium (MTB) is spreading worldwide. Three drug-resistant isolates were detected in Burmese, Hmong, and Indian immigrants currently residing in Milwaukee, Wisconsin, USA. Ion Torrent full-gene sequencing and complete genetic analysis was performed within 5 days and compared to results from traditional drug sensitivity testing (DST). Genetic characterization of seven, full-length resistance-associated genes revealed two MDR and one highly resistant strain with important drug-resistant mutations that were confirmed by traditional DST. The rapid turnaround from sample-to-sequence underscores the public health value of Ion Torrent full-gene sequencing of MDR/XDR genes from epidemiologically significant clinical isolates.
Longhorn Vaccines & Diagnostics, San Antonio, TX, USA.
L T
LT
Daum
Longhorn Vaccines & Diagnostics, San Antonio, TX, USA.
G W
GW
Fischer
City of Milwaukee Health Department, Milwaukee, WI, USA.
J
J
Sromek
City of Milwaukee Health Department, Milwaukee, WI, USA.
M
M
Khubbar
City of Milwaukee Health Department, Milwaukee, WI, USA.
P
P
Hunter
City of Milwaukee Health Department, Milwaukee, WI, USA.
M S
MS
Gradus
City of Milwaukee Health Department, Milwaukee, WI, USA.
S
S
Bhattacharyya
D000995:Antitubercular Agents; D001426:Bacterial Proteins / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods*; D002681:China / Q000453:epidemiology; D024901:Drug Resistance, Multiple, Bacterial; D054242:Emigrants and Immigrants; D016680:Genome, Bacterial; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D007194:India / Q000453:epidemiology; D008969:Molecular Sequence Data; D002052:Myanmar / Q000453:epidemiology; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D018088:Tuberculosis, Multidrug-Resistant / Q000453:epidemiology / Q000382:microbiology*; D014922:Wisconsin / Q000453:epidemiology
D000995:Antitubercular Agents; D001426:Bacterial Proteins