Actin structure-dependent stepping of myosin 5a and 10 during processive movement.
How myosin 10, an unconventional myosin, walks processively along actin is still controversial. Here, we used single molecule fluorescence techniques, TIRF and FIONA, to study the motility and the stepping mechanism of dimerized myosin 10 heavy-meromyosin-like fragment on both single actin filaments and two-dimensional F-actin rafts cross-linked by fascin or α-actinin. As a control, we also tracked and analyzed the stepping behavior of the well characterized processive motor myosin 5a. We have shown that myosin 10 moves processively along both single actin filaments and F-actin rafts with a step size of 31 nm. Moreover, myosin 10 moves more processively on fascin-F-actin rafts than on α-actinin-F-actin rafts, whereas myosin 5a shows no such selectivity. Finally, on fascin-F-actin rafts, myosin 10 has more frequent side steps to adjacent actin filaments than myosin 5a in the F-actin rafts. Together, these results reveal further single molecule features of myosin 10 on various actin structures, which may help to understand its cellular functions.
Department of Physics and Astronomy, Wayne State University, Detroit, Michigan, United States of America.
Jianjun
J
Bao
Daniel
D
Huck
Laura K
LK
Gunther
James R
JR
Sellers
Takeshi
T
Sakamoto
D008841:Actin Cytoskeleton / Q000737:chemistry / Q000502:physiology; D000199:Actins / Q000737:chemistry* / Q000502:physiology* / Q000648:ultrastructure; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000502:physiology; D008840:Microfilament Proteins / Q000737:chemistry / Q000502:physiology; D008954:Models, Biological; D009218:Myosins / Q000737:chemistry* / Q000502:physiology*; D011817:Rabbits
D000199:Actins; D002352:Carrier Proteins; D008840:Microfilament Proteins; C020324:fascin; D009218:Myosins