[The use of human papillomavirus genotying kit based on gene chip technology for detecting and typing of human papillomavirus].
OBJECTIVE
To evaluate a new human papillomavirus (HPV) genotyping technique based on gene chip technology (HPG) for HPV genotyping and its clinical efficacy.


METHODS
HPV genotyping (HPG) test, hybrid capture II (HC2) test and DNA sequencing assay were performed in 151 patients aged 20-75 years with diagnosis of chronic cervicitis or abnormal vaginal bleeding. The cervical specimens were collected from cervical epithelium. All the cervical samples were analyzed by the HPG test, HC2 test and DNA sequencing. The clinical efficacy of the HPG test was analyzed.


RESULTS
The consistent rate between HPG test and HC2 test was 87.42% (kappa = 0.75, P < 0.05). When DNA sequencing assay was regarding as the final test result, the sensitivity and specificity of HPG test for high risk HPV were 100% and 96.49%, respectively. The consistent rate between HPG test and direct DNA sequencing was 98.70% (kappa = 0.97, P < 0.05). The most common six HPV genotypes detected by HPG test were HPV 16 (13.25%), 58 (11.92%), 52 (11.92%), 31 (6.62%) 39 (5.96%), 33 (5.96%) in descending order of frequency. The incidence of multiple-types infection detected by HPG test was 23.84%.


CONCLUSIONS
HPG test is a rapid and accurate test for HPV genotyping which could detect 29 types of HPV infection at one time. It is suitable for cervical HPV infection screening in clinic.
Laboratory of Cell and Gene Therapy, West China Institute of Women and Children's Health, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Xiao-Li
XL
He
Hong-Qian
HQ
Liu
Bin
B
He
Pei
P
Yang
He
H
Wang
Shan-Ling
SL
Liu
D000328:Adult; D000368:Aged; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D061809:Human Papillomavirus DNA Tests / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics; D030361:Papillomavirus Infections / Q000821:virology*; D011933:Reagent Kits, Diagnostic; D002575:Uterine Cervicitis / Q000821:virology*; D055815:Young Adult
D015344:DNA Probes, HPV; D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic