Moscatilin induces apoptosis and mitotic catastrophe in human esophageal cancer cells.
Moscatilin, a bibenzyl derivative from the orchid Dendrobium loddigesii, has been shown to possess anticancer activity. We examined the effect of moscatilin on human esophageal cancer cells, including squamous cell carcinoma (SCC) and adenocarcinoma (ADC) cells and its possible mechanisms. Moscatilin suppressed the growth of both the histological cell lines in a dose- and time-dependent manner. Morphological changes indicative of apoptosis and mitotic catastrophe were observed following moscatilin treatment. The population of cells in the sub-G1 phase and polyploidy phase significantly increased after treatment. Immunofluorescence revealed multipolar mitosis and subsequent multinucleation in moscatilin-treated cells, indicating the development of mitotic catastrophe. Western blot showed a marked increase in expressions of polo-like kinase 1 and cyclin B1 after exposure to moscatilin. In conclusion, moscatilin inhibits growth and induces apoptosis and mitotic catastrophe in human esophageal SCC- and ADC-derived cell lines, indicating that moscatilin has broad potential against esophageal cancer.
1 Department of Radiation Oncology in Zhongxing Branch, Taipei City Hospital , Taipei, Taiwan .
Chien-An
CA
Chen
Chien-Chih
CC
Chen
Chien-Chang
CC
Shen
Hen-Hong
HH
Chang
Yu-Jen
YJ
Chen
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001593:Benzyl Compounds / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D045744:Cell Line, Tumor; D056744:Cyclin B1 / Q000235:genetics / Q000378:metabolism; D031667:Dendrobium / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004938:Esophageal Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D000077277:Esophageal Squamous Cell Carcinoma; D006801:Humans; D008938:Mitosis / Q000187:drug effects*
D000970:Antineoplastic Agents; D001593:Benzyl Compounds; D056744:Cyclin B1; D004365:Drugs, Chinese Herbal; C071845:dendrophenol