Genetically encoded FRET indicators for live-cell imaging of histone acetylation.
Histone acetylation is dynamically and reversibly controlled by histone acetyltransferases and deacetylases during cellular events such as cell division and differentiation. However, the dynamics of histone modifications in living cells are poorly understood because of the lack of experimental tools to monitor them in a real-time fashion. Herein, we introduce Förster/fluorescence resonance energy transfer (FRET)-based indicators to visualize acetylation of histone H4, and describe a protocol for live-cell imaging with high spatiotemporal resolution.
Chemical Genetics Laboratory/Chemical Genomics Research Group, RIKEN Advanced Science Institute, Wako, Japan.
Kazuki
K
Sasaki
Minoru
M
Yoshida
D000107:Acetylation; D000818:Animals; D019556:COS Cells; D002470:Cell Survival; D002522:Chlorocebus aethiops; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D005818:Genetic Engineering; D006657:Histones / Q000378:metabolism*; D057054:Molecular Imaging / Q000379:methods*; D038761:Photobleaching; D014162:Transfection
D005456:Fluorescent Dyes; D006657:Histones