Selection of Pseudomonas aeruginosa reference genes for RT-qPCR analysis from sputum of cystic fibrosis patients.
The prerequisite to monitor gene expression is the selection of reference genes for normalization of RT-qPCR results. Using 13 sputum samples collected from 9 CF patients, we demonstrated that PA2875 and PA3340 are better reference genes than the previously used clpX and oprL genes.
Univ. Bordeaux, Biotechnologie des Protéines Recombinantes à Visée Santé, EA 4135, F-33000 Bordeaux, France. Electronic address: patricia.costaglioli@ipb.fr.
Patricia
P
Costaglioli
Christophe
C
Barthe
Michael
M
Fayon
Naik
N
Christoflour
Stéphanie
S
Bui
Laurence
L
Derlich
Philippe
P
Domblides
Marc
M
Crouzet
Sébastien
S
Vilain
Bertrand
B
Garbay
D003550:Cystic Fibrosis / Q000382:microbiology*; D020869:Gene Expression Profiling / Q000379:methods; D005798:Genes, Bacterial; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D013183:Sputum / Q000382:microbiology*
Cystic fibrosis; Pseudomonas aeruginosa; RT-qPCR; Reference genes; Sputum