Voltammetric detection of adenosine-5'-diphosphate with a carbon paste electrode modified by a hydroxyl functionalized imidazolium-based ionic liquid.
A hydroxyl functionalized ionic liquid (IL) 1-(3-chloro-2-hydroxy-propyl)-3-methylimidazolium tetrafluoroborate (PMIMBF4) was used for the preparation of bulky-modified carbon paste electrode (IL-CPE), which was applied to the sensitive detection of adenosine-5'-diphosphate (ADP) in a pH 5.0 Britton-Robinson (B-R) buffer solution. IL-CPE is demonstrated to promote the oxidation of ADP with strong electrocatalytic ability. An irreversible oxidation peak appeared with adsorption-controlled process and enhanced electrochemical response on the IL-CPE. The electrode reaction parameters such as electron transfer coefficient (α) and electron transfer number (n) of ADP were calculated respectively. By using differential pulse votammetry the oxidation peak current was linearly dependent on the ADP concentration in the range from 10.0 to 1000.0 µmol/L with the detection limit as 3.23 µmol/L (3σ). The proposed method showed good selectivity to the ADP detection without the interferences of coexisting substances, which could be further used for the synthetic sample detection.
Yaqing
Y
Guo
Song
S
Hu
Xiaowei
X
Qi
Jun
J
Xiang
Wei
W
Sun