Anti-wrinkle and anti-inflammatory effects of active garlic components and the inhibition of MMPs via NF-κB signaling.
Skin aging is a multisystem degenerative process caused by several factors, such as, UV irradiation, stress, and smoke. Furthermore, wrinkle formation is a striking feature of photoaging and is associated with oxidative stress and inflammatory response. In the present study, we investigated whether caffeic acid, S-allyl cysteine, and uracil, which were isolated from garlic, modulate UVB-induced wrinkle formation and effect the expression of matrix-metalloproteinase (MMP) and NF-κB signaling. The results obtained showed that all three compounds significantly inhibited the degradation of type І procollagen and the expressions of MMPs in vivo and attenuated the histological collagen fiber disorder and oxidative stress in vivo. Furthermore, caffeic acid and S-allyl cysteine were found to decrease oxidative stress and inflammation by modulating the activities of NF-κB and AP-1, and uracil exhibited an indirect anti-oxidant effect by suppressing cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expressions levels and downregulating transcriptional factors. These results suggest that the anti-wrinkle effects of caffeic acid, S-allyl cysteine, and uracil are due to anti-oxidant and/or anti-inflammatory effects. Summarizing, caffeic acid, S-allyl cysteine, and uracil inhibited UVB-induced wrinkle formation by modulating MMP via NF-κB signaling.
Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University, Busan, Republic of Korea.
So Ra
SR
Kim
Yu Ri
YR
Jung
Hye Jin
HJ
An
Dae Hyun
DH
Kim
Eun Ji
EJ
Jang
Yeon Ja
YJ
Choi
Kyoung Mi
KM
Moon
Min Hi
MH
Park
Chan Hum
CH
Park
Ki Wung
KW
Chung
Ha Ram
HR
Bae
Yung Whan
YW
Choi
Nam Deuk
ND
Kim
Hae Young
HY
Chung
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000627:therapeutic use*; D002109:Caffeic Acids / Q000627:therapeutic use; D003545:Cysteine / Q000031:analogs & derivatives / Q000627:therapeutic use; D005737:Garlic / Q000737:chemistry*; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D015595:Skin Aging / Q000187:drug effects* / Q000528:radiation effects; D014466:Ultraviolet Rays; D014498:Uracil / Q000627:therapeutic use
D000893:Anti-Inflammatory Agents; D002109:Caffeic Acids; D016328:NF-kappa B; D014498:Uracil; C065299:S-allylcysteine; D020782:Matrix Metalloproteinases; D003545:Cysteine; C040048:caffeic acid