Impact of organic solvent additive on the integrity of plasma samples in bioanalysis by LC-MS/MS.
BACKGROUND
Matrix effects are one of the major drawbacks of ESI-MS/MS. It is majorly caused by lipids in plasma, which can be overcome by using different extraction techniques.


RESULTS
In this investigation, a major matrix effect was observed in samples containing a co-administered drug. Unknown compounds appeared over time in the human plasma samples spiked with co-administered drug creating major ion suppression. The changes in matrix integrity were associated with the organic solvent content in the plasma samples.


CONCLUSIONS
The amount and type of organic solvent added to human plasma along with the storage conditions must be carefully determined during method development in order not to impact quantitation.
Algorithme Pharma, Inc., 575 Armand-Frappier, Laval, QC, Canada.
Catherine
C
Dicaire
Jean-Nicholas
JN
Mess
Milton
M
Furtado
Annik
A
Bergeron
Fabio
F
Garofolo
D002851:Chromatography, High Pressure Liquid / Q000592:standards; D006801:Humans; D008055:Lipids / Q000737:chemistry; D009020:Morphine / Q000097:blood; D009022:Morphine Derivatives / Q000097:blood; D009271:Naltrexone / Q000737:chemistry; D009930:Organic Chemicals / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000097:blood*; D011786:Quality Control; D012015:Reference Standards; D012997:Solvents / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000592:standards
D008055:Lipids; D009022:Morphine Derivatives; D009930:Organic Chemicals; D004364:Pharmaceutical Preparations; D012997:Solvents; D009271:Naltrexone; C035349:morphine-6-glucuronide; D009020:Morphine