Make no bones about it: cells could soon be reprogrammed to grow replacement bones?
Recent developments in nuclear reprogramming allow the generation of patient-matched stem cells with broad potential for applications in cell therapies, disease modeling and drug discovery. An increasing body of work is reporting the derivation of lineage-specific progenitors from human-induced pluripotent stem cells (hiPSCs), which could in the near future be used to engineer personalized tissue substitutes, including those for reconstructive therapies of bone. Although the potential clinical impact of such technology is not arguable, significant challenges remain to be addressed before hiPSC-derived progenitors can be employed to engineer bone substitutes of clinical relevance. The most important challenge is indeed the construction of personalized multicellular bone substitutes for the treatment of complex skeletal defects that integrate fast, are immune tolerated and display biofunctionality and long-term safety. As recent studies suggest, the merging of iPSC technology with advanced biomaterials and bioreactor technologies offers a way to generate bone substitutes in a controllable, automated manner with potential to meet the needs for scale-up and requirements for translation into clinical practice. It is only via the use of state-of-the-art cell culture technologies, process automation under GMP-compliant conditions, application of appropriate engineering strategies and compliance with regulatory policies that personalized lab-made bone grafts can start being used to treat human patients.
The New York Stem Cell Foundation Research Institute , 1995 Broadway, NY 10032 , USA +1 212 851 5422 ; +1 212 851 5423 ; gmdepeppo@nyscf.org.
Giuseppe Maria
GM
de Peppo
Darja
D
Marolt
D000818:Animals; D001861:Bone Regeneration / Q000235:genetics; D018786:Bone Substitutes / Q000627:therapeutic use*; D001842:Bone and Bones / Q000378:metabolism / Q000601:surgery*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism / Q000637:transplantation*; D016348:Osseointegration; D044968:Regenerative Medicine / Q000379:methods*; D023822:Tissue Engineering
D018786:Bone Substitutes