In vitro evaluation of encapsulated primary rat hepatocytes pre- and post-cryopreservation at -80°C and in liquid nitrogen.
Encapsulation techniques have the potential to protect hepatocytes from cryoinjury. In this study, we comparatively evaluated the viability and metabolic function of primary rat hepatocytes encapsulated in calcium alginate microbeads, in chitosan tripolyphosphate beads, and in three-layered alginate-chitosan-alginate (ACA) microcapsules, before and after cryopreservation at -80°C and in liquid nitrogen (LN2) for 1 and 3 months. Findings demonstrated that LN2 was atop of -80°C in regard to preservation of viability (> 90%) and hepatic functions. LN2-cryopreserved hepatocytes encapsulated in ACA microcapsules retained metabolic function post-thawing, with > 90% of the albumin, total protein and urea syntheses activities, and > 80% of oxidative function.
Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science, and Ankara University Stem Cell Institute , Ankara , Turkey.
Serap
S
Durkut
A Eser
AE
Elçin
Y Murat
YM
Elçin
D000418:Albumins / Q000096:biosynthesis; D000464:Alginates / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002214:Capsules / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D018914:Cells, Immobilized; D048271:Chitosan / Q000031:analogs & derivatives / Q000494:pharmacology*; D015925:Cryopreservation; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005615:Freezing; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D019164:Liver, Artificial; D008297:Male; D009584:Nitrogen; D010085:Oxidative Phosphorylation; D061251:Primary Cell Culture; D051381:Rats; D017208:Rats, Wistar; D014508:Urea / Q000378:metabolism
D000418:Albumins; D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D014508:Urea; D048271:Chitosan; D003577:Cytochrome P-450 Enzyme System; D009584:Nitrogen
CYP450 activity; alginate; bioartificial liver; chitosan; cryopreservation; encapsulation; hepatocyte; synthetic metabolism; xenobiotic metabolism