Characterization of a layered methylene blue/vanadium oxide nanocomposite and its application in a reagentless H2O2 biosensor.
Layered nanocomposite of methylene blue (MB)-intercalated vanadium oxide was obtained through a simple hydrothermal synthesis method using MB, V2O5, and NaI as starting materials. The intercalation reaction was proven to be successful using X-ray diffraction pattern. The MB-V2O5 nanocomposite was characterized using a scanning electron micrograph, infrared spectra, thermogravimetric analysis, UV spectra, and electrochemical measurements. The intercalated MB cations showed a fine diffusion-controlled electrochemical redox process and facilitated the immobilized horseradish peroxidase's (HRP) good catalytic reduction upon H2O2. The as-prepared MB-V2O5/HRP biosensor showed a linear response to H2O2 over a range from 2.0 × 10(-6) to 9.5 × 10(-5) M with a detection limit of 9.7 × 10(-7) M (S/N ratio = 3).
Department of Chemical Engineering, Huaihai Institute of Technology, Lianyungang, 222005, People's Republic of China.
Xiaobo
X
Zhang
Dan
D
Li
Fujun
F
Yin
Junyan
J
Gong
Xujie
X
Yang
Zhiwei
Z
Tong
Xingyou
X
Xu
D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D008751:Methylene Blue / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D053761:Nanocomposites / Q000737:chemistry*; D013997:Time Factors; D017968:Vanadium Compounds / Q000737:chemistry*
D004800:Enzymes, Immobilized; D017968:Vanadium Compounds; D006861:Hydrogen Peroxide; C066075:vanadium pentoxide; D006735:Horseradish Peroxidase; D008751:Methylene Blue