Tuning InAs nanowire density for HEK293 cell viability, adhesion, and morphology: perspectives for nanowire-based biosensors.
Arrays of nanowires (NWs) are currently being established as vehicles for molecule delivery and electrical- and fluorescence-based platforms in the development of biosensors. It is conceivable that NW-based biosensors can be optimized through increased understanding of how the nanotopography influences the interfaced biological material. Using state-of-the-art homogenous NW arrays allow for a systematic investigation of how the broad range of NW densities used by the community influences cells. Here it is demonstrated that indium arsenide NW arrays provide a cell-promoting surface, which affects both cell division and focal adhesion up-regulation. Furthermore, a systematic variation in NW spacing affects both the detailed cell morphology and adhesion properties, where the latter can be predicted based on changes in free-energy states using the proposed theoretical model. As the NW density influences cellular parameters, such as cell size and adhesion tightness, it will be important to take NW density into consideration in the continued development of NW-based platforms for cellular applications, such as molecule delivery and electrical measurements.
Bionanotechnology and Nanomedicine Laboratory, Department of Chemistry and Nano-science Center, University of Copenhagen , Universitetsparken 5, DK-2100, Copenhagen, Denmark.
Sara
S
Bonde
Trine
T
Berthing
Morten Hannibal
MH
Madsen
Tor Kristian
TK
Andersen
Nina
N
Buch-Månson
Lei
L
Guo
Xiaomei
X
Li
Florent
F
Badique
Karine
K
Anselme
Jesper
J
Nygård
Karen L
KL
Martinez
D001152:Arsenicals / Q000737:chemistry*; D015374:Biosensing Techniques; D002448:Cell Adhesion; D002470:Cell Survival; D057809:HEK293 Cells; D006801:Humans; D007204:Indium / Q000737:chemistry*; D053770:Nanowires / Q000737:chemistry*; D013499:Surface Properties
D001152:Arsenicals; D007204:Indium; C076773:indium arsenide