Kolente virus, a rhabdovirus species isolated from ticks and bats in the Republic of Guinea.
Kolente virus (KOLEV) is a rhabdovirus originally isolated from ticks and a bat in Guinea, West Africa, in 1985. Although tests at the time of isolation suggested that KOLEV is a novel rhabdovirus, it has remained largely uncharacterized. We assembled the complete genome sequence of the prototype strain DakAr K7292, which was found to encode the five canonical rhabdovirus structural proteins (N, P, M, G and L) with alternative ORFs (>180 nt) in the P and L genes. Serologically, KOLEV exhibited a weak antigenic relationship with Barur and Fukuoka viruses in the Kern Canyon group. Phylogenetic analysis revealed that KOLEV represents a distinct and divergent lineage that shows no clear relationship to any rhabdovirus except Oita virus, although with limited phylogenetic resolution. In summary, KOLEV represents a novel species in the family Rhabdoviridae.
Center for Vaccine Research, Department of Computational and Systems Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Elodie
E
Ghedin
Center for Vaccine Research, Department of Computational and Systems Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Matthew B
MB
Rogers
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA.
Steven G
SG
Widen
Center for Biodefense and Emerging Infectious Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
Hilda
H
Guzman
Center for Biodefense and Emerging Infectious Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
Amelia P A
APA
Travassos da Rosa
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA.
Thomas G
TG
Wood
Center for Vaccine Research, Department of Computational and Systems Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Adam
A
Fitch
Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA.|Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX, USA.|Center for Biodefense and Emerging Infectious Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
Vsevolod
V
Popov
Fogarty International Center, National Institutes of Health, Bethesda, MD, USA.|Sydney Emerging Infections & Biosecurity Institute, School of Biological Sciences and Sydney Medical School, University of Sydney, Sydney, NSW 2006, Australia.
Edward C
EC
Holmes
CSIRO Animal, Food and Health Sciences, Australian Animal Health Laboratory, Geelong, VIC 3220, Australia.
Peter J
PJ
Walker
Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA.|Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX, USA.|Center for Biodefense and Emerging Infectious Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
Nikos
N
Vasilakis
Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA.|Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX, USA.|Center for Biodefense and Emerging Infectious Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
Robert B
RB
Tesh
D000818:Animals; D002460:Cell Line; D002685:Chiroptera / Q000821:virology*; D016679:Genome, Viral; D016701:Guinea; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012209:Rhabdoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013987:Ticks / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D012367:RNA, Viral; D014764:Viral Proteins