Induction of apoptosis and cell cycle arrest in A549 human lung adenocarcinoma cells by surface-capping selenium nanoparticles: an effect enhanced by polysaccharide-protein complexes from Polyporus rhinocerus.
Surface-capping agents play key roles in cellular uptake and biological activity of functional nanomaterials. In the present study, functionalized selenium nanoparticles (SeNPs) have been successfully synthesized using Polyporus rhinocerus water-soluble polysaccharide-protein complexes (PRW) as the capping agent during the reduction of selenium salts. The acquired monodisperse, spherical PRW-SeNPs presented desirable size distribution and stability in the solution. Moreover, PRW surface decoration significantly enhanced the cellular uptake of SeNPs via endocytosis. Exposure to PRW-SeNPs significantly inhibited the growth of A549 cells through induction of apoptosis and G2/M phase arrest (IC50 = 4.06 ± 0.25 μM) supported by an increase of sub-G1 and G2/M phase cell populations, DNA fragmentation, and chromatin condensation. Caspase-3/8 activation induced by PRW-SeNPs indicated that the activation of death receptors was the main cause of PRW-SeNP-induced apoptosis. Collectively, the results suggest that it is highly efficient to use PRW as a surface decorator of SeNPs to enhance cellular uptake and anticancer efficacy, and the PRW-SeNPs are potential chemopreventive agents for lung cancer therapy.
Department of Chemistry, Jinan University , Guangzhou 510632, China.
Hualian
H
Wu
Huili
H
Zhu
Xiaoling
X
Li
Zumei
Z
Liu
Wenjie
W
Zheng
Tianfeng
T
Chen
Bo
B
Yu
Ka-Hing
KH
Wong
D000230:Adenocarcinoma / Q000188:drug therapy* / Q000503:physiopathology; D000077192:Adenocarcinoma of Lung; D000970:Antineoplastic Agents / Q000737:chemistry / Q000493:pharmacokinetics; D017209:Apoptosis / Q000187:drug effects*; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D045744:Cell Line, Tumor; D053938:DNA Fragmentation / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry*; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000503:physiopathology*; D053758:Nanoparticles / Q000737:chemistry; D010316:Particle Size; D055492:Polyporus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D012643:Selenium / Q000737:chemistry / Q000493:pharmacokinetics*
D000970:Antineoplastic Agents; D004337:Drug Carriers; D005656:Fungal Proteins; D011134:Polysaccharides; D012643:Selenium