Effects of α-eleostearic acid on asolectin liposomes dynamics: relevance to its antioxidant activity.
In this study, the effect of α-eleostearic acid (α-ESA) on the lipid peroxidation of soybean asolectin (ASO) liposomes was investigated. This effect was correlated to changes caused by the fatty acid in the membrane dynamics. The influence of α-ESA on the dynamic properties of liposomes, such as hydration, mobility and order, were followed by horizontal attenuated total reflection Fourier transform infrared spectroscopy (HATR-FTIR), nuclear magnetic resonance (NMR), differential scanning calorimetry (DSC) and UV-vis techniques. The α-ESA showed an in vitro antioxidant activity against the damage induced by hydroxyl radical (OH) in ASO liposomes. The analysis of HATR-FTIR frequency shifts and bandwidths and (1)H NMR spin-lattice relaxation times, related to specific lipid groups, showed that α-ESA causes an ordering effect on the polar and interfacial regions of ASO liposomes, which may restrict the OH diffusion in the membrane. The DSC enthalpy variation analysis suggested that the fatty acid promoted a disordering effect on lipid hydrophobic regions, which may facilitate interactions between the reactive specie and α-ESA. Turbidity results showed that α-ESA induces a global disordering effect on ASO liposomes, which may be attributed to a change in the lipid geometry and shape. Results of this study may allow a more complete view of α-ESA antioxidant mode of action against OH, considering its influence on the membrane dynamics.
Research Group of Membrane Molecular Interactions, School of Chemical and Food Engineering, Post-graduation Program in Technological and Environmental Chemistry, Federal University of Rio Grande, Av. Itália, km 8, Campus Carreiros, Rio Grande-RS 96203-900, Brazil.
Robson Simplício
RS
de Sousa
Alessandro Oliveira
AO
de Moraes Nogueira
Viviane Gobel
VG
Marques
Rosilene Maria
RM
Clementin
Vânia Rodrigues
VR
de Lima
D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D008042:Linolenic Acids / Q000737:chemistry / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism*; D013025:Glycine max / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics
D000975:Antioxidants; D008042:Linolenic Acids; D008081:Liposomes; D010713:Phosphatidylcholines; C004934:eleostearic acid; D017665:Hydroxyl Radical; C024990:asolectin
()OH; ASO; Asolectin; DSC; FTIR; Fourier transform infrared spectroscopy; HATR-FTIR; Hydroxyl radical; Lipid peroxidation; Liposomes; MLV; NMR; S.D.; SPC; TBARS; TCA; correlation time; differential scanning calorimetry; enthalpy variation; horizontal attenuated total reflection-Fourier transform infrared spectroscopy; hydroxyl radical; multilamellar large vesicles; nuclear magnetic resonance; soybean asolectin; soybean phosphatidylcholine; standard deviation; stretching vibration; thiobarbituric acid reactive substances; trichloroacetic acid; ΔH; α-ESA; α-Eleostearic acid; α-eleostearic acid; ν; τ