Amplification and deletion of the RAPH1 gene in breast cancer patients.
Lamellipodin protein (Lpd), encoded by the RAPH1 gene, modulates the assembly of actin cytoskeleton through its binding to the Ena/VASPs proteins, and acts in cellular motility and lamelipodial protrusion. The region where RAPH1 gene is located (2q33) is deleted in various types of cancer and the gene expression changes in tumors when compared to normal tissues. Amplifications and deletions of the RAPH1 gene were investigated in breast carcinoma samples, in order to determine the possible relationship of the gene with breast cancer tumorigenesis and lymph node metastasis. RAPH1 gene alterations were determined by relative quantification, standard curve method using Real-time PCR technique in samples of tumor and peripheral blood from 52 patients. Regression and correlation analyses were conducted using gene alterations and clinicopathological data. All samples analyzed were altered, with 63.5 % deletion cases and 36.5 % amplification cases. The logistic regression and correlation analysis with clinicopathological data did not show significant results. The results suggest that although the RAPH1 gene was deleted or amplified in all samples, the Lpd does not seem to play a major role in tumorigenesis of mammary carcinomas and probably other proteins, also involved in the process of cellular motility and metastasis, are acting more effectively for or against the migration of breast tumor cells.
Genetics Department, Federal University of Paraná, Curitiba, PR, Brazil.
Meire S
MS
Batistela
Dellyana R
DR
Boberg
Fabiana A
FA
Andrade
Michelli
M
Pecharki
Enilze M
EM
de S F Ribeiro
Iglenir J
IJ
Cavalli
Rubens S
RS
Lima
Cícero A
CA
Urban
Lupe
L
Furtado-Alle
Ricardo L R
RL
Souza
D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002352:Carrier Proteins / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D017353:Gene Deletion; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged
D002352:Carrier Proteins; D008565:Membrane Proteins; C493339:RAPH1 protein, human