Development of a general methodology for labelling peptide-morpholino oligonucleotide conjugates using alkyne-azide click chemistry.
We describe a general methodology for fluorescent labelling of peptide conjugates of phosphorodiamidate morpholino oligonucleotides (PMOs) by alkyne functionalization of peptides, subsequent conjugation to PMOs and labelling with a fluorescent compound (Cy5-azide). Two peptide-PMO (PPMO) examples are shown. No detrimental effect of such labelled PMOs was seen in a biological assay.
MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK. mgait@mrc-lmb.cam.ac.uk.
Fazel
F
Shabanpoor
Michael J
MJ
Gait
D000480:Alkynes / Q000737:chemistry*; D001386:Azides / Q000737:chemistry*; D057930:Click Chemistry; D005453:Fluorescence; D015394:Molecular Structure; D060172:Morpholinos / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D013194:Staining and Labeling
D000480:Alkynes; D001386:Azides; D060172:Morpholinos; D010455:Peptides