A cellular and metabolic assessment of the thermal stress responses in the endemic gastropod Benedictia limnaeoides ongurensis from Lake Baikal.
Our objective was to determine if the Lake Baikal endemic gastropod Benedictia limnaeoides ongurensis, which inhabits in stable cold waters expresses a thermal stress response. We hypothesized that the evolution of this species in the stable cold waters of Lake Baikal resulted in a reduction of its thermal stress-response mechanisms at the biochemical and cellular levels. Contrary to our hypothesis, our results show that exposure to a thermal challenge activates the cellular and biochemical mechanisms of thermal resistance, such as heat shock proteins and antioxidative enzymes, and alters energetic metabolism in B. limnaeoides ongurensis. Thermal stress caused the elevation of heat shock protein 70 and the products of anaerobic glycolysis together with the depletion of glucose and phosphagens in the studied species. Thus, a temperature increase activates the complex biochemical system of stress response and alters the energetic metabolism in this endemic Baikal gastropod. It is concluded that the deepwater Lake Baikal endemic gastropod B. limnaeoides ongurensis retains the ability to activate well-developed biochemical stress-response mechanisms when exposed to a thermal challenge.
Irkutsk State University, Karl Marx St. 1, 664003 Irkutsk, Russia.
Denis V
DV
Axenov-Gribanov
Daria S
DS
Bedulina
Zhanna M
ZM
Shatilina
Yulia A
YA
Lubyaga
Kseniya P
KP
Vereshchagina
Maxim A
MA
Timofeyev
D000818:Animals; D049851:Gastropoda / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology; D018869:Heat-Shock Response; D060106:Lakes; D012426:Russia; D013696:Temperature
Benedictia limnaeoides ongurensis; Gastropoda; HSP70; Lake Baikal; Stress-response