Growth and characterization of different human rhinovirus C types in three-dimensional human airway epithelia reconstituted in vitro.
New molecular diagnostic tools have recently allowed the discovery of human rhinovirus species C (HRV-C) that may be overrepresented in children with lower respiratory tract complications. Unlike HRV-A and HRV-B, HRV-C cannot be propagated in conventional immortalized cell lines and their biological properties have been difficult to study. Recent studies have described the successful amplification of HRV-C15, HRV-C11, and HRV-C41 in sinus mucosal organ cultures and in fully differentiated human airway epithelial cells. Consistent with these studies, we report that a panel of clinical HRV-C specimens including HRV-C2, HRV-C7, HRV-C12, HRV-C15, and HRV-C29 types were all capable of mediating productive infection in reconstituted 3D human primary upper airway epithelial tissues and that the virions enter and exit preferentially through the apical surface. Similar to HRV-A and HRV-B, our data support the acid sensitivity of HRV-C. We observed also that the optimum temperature requirement during HRV-C growth may be type-dependent.
Laboratory of Virology, Division of Infectious Diseases and Division of Laboratory Medicine, University of Geneva Hospitals, 4 Rue Gabrielle-Perret-Gentil, 1211 Geneva 14, Switzerland. Electronic address: Caroline.Tapparel@hcuge.ch.
Caroline
C
Tapparel
Komla
K
Sobo
Samuel
S
Constant
Song
S
Huang
Sandra
S
Van Belle
Laurent
L
Kaiser
D006801:Humans; D009924:Organ Culture Techniques / Q000379:methods; D010850:Picornaviridae Infections / Q000821:virology; D020545:Respiratory Mucosa / Q000821:virology*; D012229:Rhinovirus / Q000254:growth & development* / Q000302:isolation & purification / Q000502:physiology; D014773:Virology / Q000379:methods; D053586:Virus Internalization; D057074:Virus Release
Acidity; Apical; Basal; Human rhinovirus C; Reconstituted tissue culture; Temperature sensitivity; Types