Geomicrobium sediminis sp. nov., a novel bacterium isolated from a sediment sample collected from the South China Sea, and emended description of the genus Geomicrobium.
A novel bacterium, designated YIM M13075(T), was isolated from a sediment sample collected from the South China Sea. Growth occurred from 4 to 45 °C (optimum 28 °C), pH 6.0-11.0 (optimum pH 8.0). The strain formed yellow-cream colonies after 5 days incubation on TSA modified with 5 % NaCl medium at 28 °C. Cells were Gram-positive, short rods and motile. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YIM M13075(T) was affiliated with the genus Geomicrobium (93.5 %). The strain YIM M13075(T) contained meso-diaminopimelic acid in the cell wall. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The major fatty acids were iso-C15:0 and anteiso-C15:0. The predominant menaquinones were MK-7 and MK-6. The genomic DNA G+C content was 42.7 mol%. On the basis of the morphological and chemotaxonomic characteristics as well as genotypic data, strain YIM M13075(T) represents a novel species in the genus Geomicrobium, for which the name Geomicrobium sediminis sp. nov. is proposed. The type strain is YIM M13075(T) (=DSM 25540(T) =JCM 18144(T) =CCTCC AB 2013245(T)). An emended description of the genus Geomicrobium is also proposed in the light of the new data.
Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.
Zi-Jun
ZJ
Xiong
Yong-Guang
YG
Zhang
Dao-Feng
DF
Zhang
Bing-Bing
BB
Liu
Li
L
Li
Xiao-Mei
XM
Zhang
Li-Hua
LH
Xu
Wen-Jun
WJ
Li
D056449:Bacillales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D002681:China; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D003960:Diaminopimelic Acid