Volatile organic compounds characterized from grapevine (Vitis vinifera L. cv. Malbec) berries increase at pre-harvest and in response to UV-B radiation.
Ultraviolet-B solar radiation (UV-B) is an environmental signal with biological effects in plant tissues. Recent investigations have assigned a protective role of volatile organic compounds (VOCs) in plant tissues submitted to biotic and abiotic stresses. This study investigated VOCs in berries at three developmental stages (veraison, pre-harvest and harvest) of Vitis vinifera L. cv. Malbec exposed (or not) to UV-B both, in in vitro and field experiments. By Head Space-Solid Phase Micro Extraction-Gas Chromatography-Electron Impact Mass Spectrometry (HS-SPME-GC-EIMS) analysis, 10 VOCs were identified at all developmental stages: four monoterpenes, three aldehydes, two alcohols and one ketone. Monoterpenes increased at pre-harvest and in response to UV-B in both, in vitro and field conditions. UV-B also augmented levels of some aldehydes, alcohols and ketones. These results along with others from the literature suggest that UV-B induce grape berries to produce VOCs (mainly monoterpenes) that protect the tissues from UV-B itself and other abiotic and biotic stresses, and could affect the wine flavor. Higher emission of monoterpenes was observed in the field experiments as compared in vitro, suggesting the UV-B/PAR ratio is not a signal in itself.
Laboratorio de Bioquímica Vegetal, Instituto de Biología Agrícola de Mendoza, Consejo Nacional de Investigaciones Científicas y Tecnológicas-Universidad Nacional de Cuyo, Almirante Brown 500, M5528AHB Chacras de Coria, Argentina.
Mariana
M
Gil
Rubén
R
Bottini
Federico
F
Berli
Mariela
M
Pontin
María Fernanda
MF
Silva
Patricia
P
Piccoli
D000438:Alcohols / Q000032:analysis / Q000528:radiation effects; D000447:Aldehydes / Q000032:analysis / Q000528:radiation effects; D005638:Fruit / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D007659:Ketones / Q000032:analysis / Q000528:radiation effects; D015394:Molecular Structure; D039821:Monoterpenes / Q000032:analysis* / Q000528:radiation effects; D014466:Ultraviolet Rays; D027843:Vitis / Q000737:chemistry* / Q000528:radiation effects*; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
D000438:Alcohols; D000447:Aldehydes; D007659:Ketones; D039821:Monoterpenes; D055549:Volatile Organic Compounds
Berries; Grapevine; Terpenes; UV-B radiation; Vitaceae; Vitis vinifera L.; Volatiles