Potentiation of antibiofilm activity of amphotericin B by superoxide dismutase inhibition.
This study demonstrates a role for superoxide dismutases (Sods) in governing tolerance of Candida albicans biofilms to amphotericin B (AmB). Coincubation of C. albicans biofilms with AmB and the Sod inhibitors N,N'-diethyldithiocarbamate (DDC) or ammonium tetrathiomolybdate (ATM) resulted in reduced viable biofilm cells and increased intracellular reactive oxygen species levels as compared to incubation of biofilm cells with AmB, DDC, or ATM alone. Hence, Sod inhibitors can be used to potentiate the activity of AmB against C. albicans biofilms.
Centre of Microbial and Plant Genetics, KU, Leuven, 3001 Heverlee, Belgium.
Katrijn
K
De Brucker
Anna
A
Bink
Els
E
Meert
Bruno P A
BP
Cammue
Karin
K
Thevissen
D000666:Amphotericin B / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D004050:Ditiocarb / Q000494:pharmacology; D008982:Molybdenum / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors*
D017382:Reactive Oxygen Species; D000666:Amphotericin B; D008982:Molybdenum; C020809:tetrathiomolybdate; D004050:Ditiocarb; D013482:Superoxide Dismutase