[Study on HPLC-fingerprint-based identification of dao-di herb and non dao-di herb of scutellariae radix].
OBJECTIVE
To compare the discrepancies between chemical constituents in Dao-di herb and non Dao-di herb of Huangqin (the root of Scutellaria baicalensis), study the impact of habitat and growth pattern (including cultivated and wild Huangqin) on chemical substances of Huangqin, and then provide evidence for the identification of Dao-di herb and quality evaluation of Huangqin.


METHODS
The chemical constituents in Huangqin collected from different habitats and under different growth patterns, were analyzed using HPLC fingerprint. The fingerprints obtained were then evaluated by hierarchical clustering analysis, principal component analysis and components peak area pattern.


RESULTS
The fingerprints' chemical profiles of Dao-di herb and non Dao-di Huangqin had significant disparity. The fingerprints of modem Dao-di herb Huangqin samples originated from Chengde (Hebei Province) were significantly different from those from other habitats, though the fingerprints of the non Dao-di Huangqin collected from Chifeng (Inner Mongolia) and Chengde had high similarity to each other. The chemical characteristics of Huangqin samples collected from the habitats recorded in ancient herbals, such as Qingyang (Gansu Province), Yan'an (Shaanxi Province), Linyi (Shangdong Province), Changzhi and Jinzhong (Shanxi Province) were similar. The fingerprints of modern non Dao-di samples collected from Dingxi and Longnan (Gansu Province) and Shangluo (Shaanxi Province) had high similarity. In addition, the content of acteoside in wild Huangqin was higher than that in cultivated Huangqin.


CONCLUSIONS
Dao-di herb and non Dao-di herb of Huangqin could be distinguished using the developed HPLC fingerprints. The results obtained may provide evidence for the quality control and pharmcodynamical research of Dao-di herb and non Dao-di Huangqin.
Department of Chemical Biology, School of Pharmaceutical Science, Peking University, Beijing 100191, China.
Dan
D
Wang
Qiu-Yan
QY
Zhang
Xing-Xin
XX
Yang
Ya-Jie
YJ
Jiang
Ming-Ying
MY
Shang
Zi-Wei
ZW
Zhang
Shao-Qing
SQ
Cai
Yu-Ying
YY
Zhao
Xuan
X
Wang
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000592:standards*; D011786:Quality Control; D031364:Scutellaria baicalensis / Q000737:chemistry*
D004365:Drugs, Chinese Herbal