Analysis of gene expression during the transition to climacteric phase in carnation flowers (Dianthus caryophyllus L.).
It has been generally thought that in ethylene-sensitive plants such as carnations, senescence proceeds irreversibly once the tissues have entered the climacteric phase. While pre-climacteric petal tissues have a lower sensitivity to ethylene, these tissues are converted to the climacteric phase at a critical point during flower development. In this study, it is demonstrated that the senescence process initiated by exogenous ethylene is reversible in carnation petals. Petals treated with ethylene for 12h showed sustained inrolling and senescence, while petals treated with ethylene for 10h showed inrolling followed by recovery from inrolling. Reverse transcription-PCR analysis revealed differential expression of genes involved in ethylene biosynthesis and ethylene signalling between 10h and 12h ethylene treatment. Ethylene treatment at or beyond 12h (threshold time) decreased the mRNA levels of the receptor genes (DcETR1, DcERS1, and DcERS2) and DcCTR genes, and increased the ethylene biosynthesis genes DcACS1 and DcACO1. In contrast, ethylene treatment under the threshold time caused a transient decrease in the receptor genes and DcCTR genes, and a transient increase in DcACS1 and DcACO1. Sustained DcACS1 accumulation is correlated with decreases in DcCTR genes and increase in DcEIL3 and indicates that tissues have entered the climacteric phase and that senescence proceeds irreversibly. Inhibition of ACS (1-aminocyclopropane-1-carboxylic acid synthase) prior to 12h ethylene exposure was not able to prevent reduction in transcripts of DcCTR genes, yet suppressed transcript of DcACS1 and DcACO1. This leads to the recovery from inrolling of the petals, indicating that DcACS1 may act as a signalling molecule in senescence of flowers.
Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USA.
Byung-Chun
BC
In
Brad M
BM
Binder
Tanya G
TG
Falbel
Sara E
SE
Patterson
D029749:Dianthus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005030:Ethylenes / Q000096:biosynthesis; D035264:Flowers / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism
D005030:Ethylenes; D010940:Plant Proteins; C036216:ethylene
1-MCP; Carnation; ethylene biosynthesis; ethylene receptors; petal inrolling; senescence.