Agrobacterium-mediated transformation of the white-rot fungus Physisporinus vitreus.
The biotechnologically important white-rot fungus Physisporinus vitreus was co-cultivated with Agrobacterium tumefaciens AGL-1 carrying plasmids with nourseothricin resistance as the selectable marker gene and red fluorescence protein as a visual marker. Mitotically stable transformed isolates were obtained showing red fluorescence protein activity.
Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Applied Wood Materials, Lerchenfeldstrasse 5, CH-9014 St. Gallen, Switzerland. Electronic address: mark.schubert@empa.ch.
M
M
Schubert
C
C
Stührk
M J
MJ
Fuhr
F W M R
FW
Schwarze
D016960:Agrobacterium tumefaciens; D001487:Basidiomycota / Q000235:genetics* / Q000254:growth & development; D018920:Coculture Techniques / Q000379:methods; D018014:Gene Transfer Techniques; D005819:Genetic Markers; D008164:Luminescent Proteins / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D013309:Streptothricins; D014170:Transformation, Genetic; D000097573:Red Fluorescent Protein
D005819:Genetic Markers; D008164:Luminescent Proteins; D013309:Streptothricins; D000097573:Red Fluorescent Protein
Agrobacterium tumefaciens; Physisporinus vitreus; Red fluorescent protein (RFP); Transformation