High frequency of false-positive hepatitis C virus enzyme-linked immunosorbent assay in Rakai, Uganda.
The prevalence of hepatitis C virus (HCV) infection in sub-Saharan Africa remains unclear. We tested 1000 individuals from Rakai, Uganda, with the Ortho version 3.0 HCV enzyme-linked immunosorbent assay. All serologically positive samples were tested for HCV RNA. Seventy-six of the 1000 (7.6%) participants were HCV antibody positive; none were confirmed by detection of HCV RNA.
Department of Medicine, School of Medicine, Johns Hopkins University, Baltimore.
Caroline E
CE
Mullis
Oliver
O
Laeyendecker
Steven J
SJ
Reynolds
Ponsiano
P
Ocama
Jeffrey
J
Quinn
Iga
I
Boaz
Ronald H
RH
Gray
Gregory D
GD
Kirk
David L
DL
Thomas
Thomas C
TC
Quinn
Lara
L
Stabinski
D000328:Adult; D000704:Analysis of Variance; D004797:Enzyme-Linked Immunosorbent Assay / Q000706:statistics & numerical data*; D005189:False Positive Reactions; D005260:Female; D015658:HIV Infections / Q000097:blood / Q000209:etiology / Q000821:virology; D016174:Hepacivirus / Q000276:immunology / Q000302:isolation & purification; D006526:Hepatitis C / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology / Q000821:virology; D018937:Hepatitis C Antibodies / Q000097:blood; D006801:Humans; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D012367:RNA, Viral / Q000097:blood; D016036:Seroepidemiologic Studies; D014454:Uganda / Q000453:epidemiology; D055815:Young Adult
D018937:Hepatitis C Antibodies; D012367:RNA, Viral
Africa; ELISA; hepatitis C virus