Structure and expression of a novel compact myelin protein - small VCP-interacting protein (SVIP).
SVIP (small p97/VCP-interacting protein) was initially identified as one of many cofactors regulating the valosin containing protein (VCP), an AAA+ ATPase involved in endoplasmic-reticulum-associated protein degradation (ERAD). Our previous study showed that SVIP is expressed exclusively in the nervous system. In the present study, SVIP and VCP were seen to be co-localized in neuronal cell bodies. Interestingly, we also observed that SVIP co-localizes with myelin basic protein (MBP) in compact myelin, where VCP was absent. Furthermore, using nuclear magnetic resonance (NMR) and circular dichroism (CD) spectroscopic measurements, we determined that SVIP is an intrinsically disordered protein (IDP). However, upon binding to the surface of membranes containing a net negative charge, the helical content of SVIP increases dramatically. These findings provide structural insight into interactions between SVIP and myelin membranes.
Department of Neurology, Vanderbilt University School of Medicine, United States.
Jiawen
J
Wu
Dungeng
D
Peng
Markus
M
Voehler
Charles R
CR
Sanders
Jun
J
Li
D000595:Amino Acid Sequence; D000818:Animals; D000838:Anions / Q000737:chemistry; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis* / Q000235:genetics; D028044:Phosphate-Binding Proteins; D010957:Plasmids / Q000235:genetics; D017433:Protein Structure, Secondary; D051381:Rats; D012584:Sciatic Nerve / Q000648:ultrastructure
D000838:Anions; D002352:Carrier Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins; D009687:Nuclear Proteins; D028044:Phosphate-Binding Proteins; C569017:SVIP protein, human; C569020:SVIP protein, mouse; C475849:SVIP protein, rat
Compact myelin; Intrinsically disordered protein; Myelin basic protein; Protein–membrane interaction; Small VCP-interacting protein; Valosin containing protein