Identification of human patatin-like phospholipase domain-containing protein 1 and a mutant in human cervical cancer HeLa cells.
Recently members of mammalian patatin-like phospholipase domain containing (PNPLA) protein family have attracted attention for their critical roles in diverse aspects of lipid metabolism and signal pathway. Until now little has been known about the characteristics of PNPLA1. Here, the full length coding cDNA sequence of human PNPLA1 (hPNPLA1) was cloned for the first time, which encoded a polypeptide with 532 amino acids containing the whole patatin domain. Tissue expression profiles analysis showed that low mRNA levels of hPNPLA1 existed in various tissues, except high expression in the digestive system, bone marrow and spleen. Subcellular distribution of hPNPLA1 tagged with green fluorescence protein mainly localized to lipid droplets. Furthermore, a nonsense mutation of PNPLA1 in human cervical cancer HeLa cells was identified. The hPNPLA1 mutant encoded a protein of 412 amino acids without the C-terminal domain and did not colocalize to lipid droplets, which suggested that the C-terminal region of hPNPLA1 affected lipid droplet binding. These results identified hPNPLA1 and a mutant in HeLa cells, and provided insights into the structure and function of PNPLA1.
Key Laboratory of Molecular Biology, College of Bio-information, Chongqing University of Posts and Telecommunications, Chongqing, 400065, People's Republic of China, changpa@cqupt.edu.cn.
Ping-An
PA
Chang
Ying-Jian
YJ
Sun
Fei-Fei
FF
Huang
Wen-Zhen
WZ
Qin
Yu-Ying
YY
Chen
Xin
X
Zeng
Yi-Jun
YJ
Wu
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D005260:Female; D020869:Gene Expression Profiling; D015894:Genome, Human / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008049:Lipase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics; D009154:Mutation / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D013347:Subcellular Fractions / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*; D000217:Acyltransferases; D010740:Phospholipases
D008049:Lipase; D050505:Mutant Proteins; C549684:PNPLA1 protein, human; D000217:Acyltransferases; D010740:Phospholipases