Genetic variation and phylogenetic relationship between three serow species of the genus Capricornis based on the complete mitochondrial DNA control region sequences.
The molecular evidence of phylogenetic status regarding the Formosan serow (Capricornis swinhoei) is not robust and little is known about the genetic diversity of the Sumatran serow (Capricornis sumatraensis), which partly is due to the hardness in sample collection. Here we determined the sequences of the complete mitochondrial DNA control region (1,014 bp) of 19 Sumatran-serow individuals. Nine new haplotypes were defined based on 78 variable sites. Combined analysis with other 32 haplotypes downloaded from the public database, including 1 Sumatran-serow, 11 Formosan-serow and 20 Japanese-serow (Capricornis crispus) haplotypes, a relatively high level of nucleotide diversity was first observed in Sumatran serow (π = 0.0249). By comparative analysis with structural consensus sequences from other mammals, we have identified central, left and right domains and depicted the putative functional structure, including extend termination associated sequences and conserve sequence blocks, in mtDNA control region. The alignment of mtDNA control region revealed that both Sumatran and Japanese serow have two tandem repeats (TRs), but three TRs in Formosan serow. Phylogenetic analyses revealed that the Formosan serow is distinct species with the Japanese serow, but a sister group with the Sumatran serow. The divergence time estimated among three serow species revealed that Pleistocene climate changes and the uplift of Qinghai-Tibetan plateau might play an important role in the genetic differentiation of the serows. These results mainly provide the convinced evidence on the genetic relationship between three serow species.
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, 625014, People's Republic of China.
Wei
W
Liu
Yong-Fang
YF
Yao
Qin
Q
Yu
Qing-Yong
QY
Ni
Ming-Wang
MW
Zhang
Jian-Dong
JD
Yang
Miao-Miao
MM
Mai
Huai-Liang
HL
Xu
D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D051456:Gene Flow; D014644:Genetic Variation; D005843:Geography; D006041:Goats / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D007214:Indonesia; D019901:Locus Control Region / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000235:genetics; D010802:Phylogeny; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D004272:DNA, Mitochondrial; D009711:Nucleotides