Creation of a caspase-3 sensing system using a combination of split-GFP and split-intein.
A genetically encodable caspase-3 sensing system has been created using self-assembling split-GFP, in which a C-terminal fragment is "covalently" cyclized via a caspase-3 substrate sequence mediated by split-intein. The specific cleavage of the cyclic C-terminal fragment by caspase-3 induces the GFP reassembly and fluorescence recovery.
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai 980-8577, Japan. sakamoto@tagen.tohoku.ac.jp hiko@tagen.tohoku.ac.jp.
Seiji
S
Sakamoto
Mika
M
Terauchi
Anna
A
Hugo
Tanner
T
Kim
Yasuyuki
Y
Araki
Takehiko
T
Wada
D053148:Caspase 3 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003500:Cyclization; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000378:metabolism*; D047668:Inteins; D008958:Models, Molecular; D013379:Substrate Specificity; D046939:Synechocystis
D049452:Green Fluorescent Proteins; D053148:Caspase 3