High concentration of gadolinium ion modifying isolated rice mitochondrial biogenesis.
Mitochondria play an important role in plant growth and development, cooperating with the endoplasmic reticulum and nucleus. Gadolinium, one of the rare earth elements, is an inhibitor of stretch-activated calcium channels located on the endoplasmic reticulum and plasma membrane and has no effect on nuclear calcium variation in plant cells. We analyzed the effects of Gd3+ on mitochondria function by monitoring mitochondrial swelling, changes of membrane fluidity, and transmembrane potential collapse and by observing mitochondrial ultrastructure. We found that high concentration of Gd3+ induces rice mitochondrial dysfunction through mitochondrial permeability transition (MPT). The protection of DTT and EDTA demonstrate that Gd3+ blocks the inner membrane ion channel through thiol chelation.
Jie
J
Zhao
Jian-Cheng
JC
Jin
Zhi-Qiang
ZQ
Zhou
Cai-Fen
CF
Xia
Xiao-Gang
XG
Yang
Feng-Lei
FL
Jiang
Jie
J
Dai
Yi
Y
Liu
D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D005682:Gadolinium / Q000009:adverse effects* / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism*; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism; D000083162:Mitochondrial Permeability Transition Pore; D012275:Oryza / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism
D015220:Calcium Channels; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D010940:Plant Proteins; D005682:Gadolinium; D002118:Calcium