The modulation of attachment, growth and morphology of cancerous prostate cells by polyelectrolyte nanofilms.
The behaviour of cancerous epithelial prostatic cells (PC3) growing on polyelectrolytes (PE) coatings was compared to the behaviour of immortalized normal prostatic cells (PNT-2). The cell behaviour was evaluated and quantified in terms of initial cell attachment, growth, metabolic activity, morphometry, adhesion, apoptosis and stress related gene expression. Both the anionic PSS (poly(sodium 4-styrenesulphonate))-terminated surface and cationic PAH (poly(allylamine hydrochloride))-terminated surfaces were not cytotoxic. The initial attachment of cells was better on the PAH-terminated surface compared to fibronectin. However, the proliferation rate of PC3 cells was reduced on the PAH-terminated surface and slightly increased on the PSS coatings. Only PAH prevented the clustering phenotype of PC3 and reduced the number of focal adhesion points as compared to fibronectin or PSS coatings. In contrast, none of the PE surfaces significantly affected the biological responses of PNT-2 cells. PAH-terminating films provide a tool to preferentially modulate the growth of some cancerous phenotypes, in this case as a micro-environment that reduces the growth of metastatic PC3 cells.
CEA, iRTSV, Biologie à Grande Echelle, F-38054 Grenoble, France; Université Grenoble-Alpes, F-38000 Grenoble, France; INSERM, U1038, F-38054 Grenoble, France. Electronic address: nathalie.picollet-dhahan@cea.fr.
Nathalie
N
Picollet-D'hahan
Sophie
S
Gerbaud
Frédérique
F
Kermarrec
Jean-Pierre
JP
Alcaraz
Patricia
P
Obeid
Ricky
R
Bhajun
Laurent
L
Guyon
Eric
E
Sulpice
Philippe
P
Cinquin
Monika E
ME
Dolega
Jayesh
J
Wagh
Xavier
X
Gidrol
Donald K
DK
Martin
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D008297:Male; D008962:Models, Theoretical; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D011467:Prostate / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000188:drug therapy; D051381:Rats
D011108:Polymers
Carcinogenesis; Cell adhesion; Cell proliferation; Epithelial cells; Layer-by-layer; Polyelectrolytes