Effect of Hg, As and Pb on biomass production, photosynthetic rate, nutrients uptake and phytochelatin induction in Pfaffia glomerata.
Plantlets of Pfaffia glomerata (Spreng.) were exposed for 28 days to three different metal/metalloid (Hg, Pb and As) with different levels (Hg 1; As 25, 50, 100 and Pb 100 and 400 μM) to analyze the possible phytochelatin initiation and affects on growth and photosynthetic pigments vis-à-vis metal accumulation potential of plants. The plantlets showed significant Hg, As and Pb accumulation in roots (150, 1267.67 and 2129 μg g(-1) DW respectively); however, a low root to shoot metal translocation was observed. It was interesting to note that all tested macronutrient (Mg, K, Ca) was higher in shoots and just opposite in case of micronutrients (Cu, Fe, Zn), was recorded highest in roots. The growth of plantlets (analyzed in terms of length and dry weight) was negatively affected by various metal treatments. In addition, the level of photosynthetic pigments alters significantly in response to all metal/metalloid treatment. In response to all tested metal/metalloids in plants only As induced phytochelatins (PC2, PC3 and PC4) in roots, and in shoots, GSH was observed in all tested metal/metalloids. In conclusion, P. glomerata plantlets could not cooperatively induce phytochelatins under any of Hg and Pb levels.
Department of Biology, University Federal de Santa Maria, Camobi, Santa Maria, RS, 97105-900, Brazil, guptadk1971@gmail.com.
D K
DK
Gupta
H G
HG
Huang
F T
FT
Nicoloso
M R
MR
Schetinger
J G
JG
Farias
T Q
TQ
Li
B H N
BH
Razafindrabe
N
N
Aryal
M
M
Inouhe
D027703:Amaranthaceae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D018533:Biomass; D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D019216:Metals, Heavy / Q000633:toxicity*; D010788:Photosynthesis / Q000187:drug effects; D054811:Phytochelatins / Q000378:metabolism*; D018675:Toxicity Tests
D019216:Metals, Heavy; D002734:Chlorophyll; D002338:Carotenoids; D054811:Phytochelatins