Slow translocation of polynucleotides and their discrimination by α-hemolysin inside a single track-etched nanopore designed by atomic layer deposition.
We report the formation of a hybrid biological/artificial nanopore by the direct insertion of non-modified α-hemolysin at the entrance of a high aspect ratio (length/diameter) biomimetic nanopore. In this robust hybrid system, the protein exhibits the same polynucleotide discrimination properties as in the biological membrane and the polynucleotide dwell time is strongly increased. This nanopore is very promising for DNA sequencing applications where the high DNA translocation velocity and the fragility of the support are the main bottlenecks.
Institut Européen des Membranes, UMR5635 CNRS-UM2-ENSCM, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France. sebastien.balme@univ-montp2.fr.
Simon
S
Cabello-Aguilar
Sébastien
S
Balme
Adib Abou
AA
Chaaya
Mikhael
M
Bechelany
Emmanuel
E
Balanzat
Jean-Marc
JM
Janot
Celine
C
Pochat-Bohatier
Philippe
P
Miele
Philippe
P
Dejardin
D040761:Biomimetic Materials / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D006460:Hemolysin Proteins / Q000737:chemistry* / Q000378:metabolism; D058608:Nanopores; D011119:Polynucleotides / Q000737:chemistry*; D017422:Sequence Analysis, DNA
D006460:Hemolysin Proteins; D011119:Polynucleotides; D004247:DNA