The design and application of genetically encodable biosensors based on fluorescent proteins.
To track the activity of cellular signaling molecules within the endogenous cellular environment, researchers have developed a diverse set of genetically encodable fluorescent biosensors. These sensors, which can be targeted to specific subcellular regions to monitor specific pools of a given signaling molecule in real time, rely upon conformational changes in a sensor domain to alter the photophysical properties of green fluorescent protein (GFP) family members. In this introductory chapter, we first discuss the properties of GFP family members before turning our attention to the design and application of genetically encodable fluorescent biosensors to live cell imaging.
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Robert H
RH
Newman
Jin
J
Zhang
D015374:Biosensing Techniques / Q000379:methods*; D002470:Cell Survival; D031541:Fluorescence Resonance Energy Transfer; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics*; D008958:Models, Molecular; D011487:Protein Conformation; D015202:Protein Engineering
D008164:Luminescent Proteins