A fluorogenic probe for β-galactosidase activity imaging in living cells.
A cell permeable fluorescence turn-on probe, AcGQCy7, was developed to image β-galactosidase activity in living cells. Once internalized by β-galactosidase-expressing cells, the probe was hydrolyzed into a highly fluorescent molecule, and the fluorescent signal was retained in mitochondria for several days. This resulted in a long-lasting and strong β-galactosidase-dependent intracellular fluorescent signal with little background fluorescence in the culture media.
Department of Translational Imaging, The Methodist Hospital Research Institute, Weill Cornell Medical College, Houston, TX 77030, USA.
Junyan
J
Han
Myung Shin
MS
Han
Ching-Hsuan
CH
Tung
D002232:Carbocyanines / Q000138:chemical synthesis*; D045744:Cell Line, Tumor; D003470:Culture Media; D003584:Cytological Techniques / Q000379:methods*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006801:Humans; D007211:Indoles / Q000138:chemical synthesis*; D008856:Microscopy, Fluorescence / Q000379:methods*; D001616:beta-Galactosidase / Q000737:chemistry / Q000378:metabolism*
C000595634:AcGQCy7 compound; D002232:Carbocyanines; D003470:Culture Media; D029968:Escherichia coli Proteins; D005456:Fluorescent Dyes; C000595633:GQCy7 compound; D007211:Indoles; D001616:beta-Galactosidase