DNA-enhanced peroxidase-like activity of layered double hydroxide nanosheets and applications in H2O2 and glucose sensing.
LDH nanosheets were obtained via continuous impaction and exfoliation by herring sperm DNA molecules using a constant vibration method. DNA-LDH nanohybrids were composed by electrostatic forces and they exhibited DNA-enhanced peroxidase-like activity. The morphology and structure of DNA-LDH nanohybrids were analyzed by transmission electron microscopy (TEM), selected-area electron diffraction (SAED), X-ray diffraction (XRD), and atomic force microscopy (AFM) characterization. On the basis of the high catalytic activity of DNA/CuAl-LDH nanosheets, a rapid, sensitive, and convenient approach was developed for colorimetric detection of H2O2 and blood glucose. This method can be potentially applied in medical diagnostics and biotechnology fields.
College of Chemistry and Material Science, Shandong Agricultural University, Taian, 271018, P. R. China. ashy@sdau.edu.cn.
Lijian
L
Chen
Kaifang
K
Sun
Peipei
P
Li
Xianzhong
X
Fan
Jianchao
J
Sun
Shiyun
S
Ai
D000535:Aluminum / Q000737:chemistry; D002384:Catalysis; D003124:Colorimetry; D003300:Copper / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D049329:Nanostructures / Q000737:chemistry*; D009195:Peroxidase / Q000378:metabolism; D013696:Temperature
D003300:Copper; D004247:DNA; D006861:Hydrogen Peroxide; D000535:Aluminum; D009195:Peroxidase; D005947:Glucose