Dental pulp tissue engineering in full-length human root canals.
The clinical translation of stem-cell-based dental pulp regeneration will require the use of injectable scaffolds. Here, we tested the hypothesis that stem cells from exfoliated deciduous teeth (SHED) can generate a functional dental pulp when injected into full-length root canals. SHED survived and began to express putative markers of odontoblastic differentiation after 7 days when mixed with Puramatrix™ (peptide hydrogel), or after 14 days when mixed with recombinant human Collagen (rhCollagen) type I, and injected into the root canals of human premolars in vitro. Roots of human premolars injected with scaffolds (Puramatrix™ or rhCollagen) containing SHED were implanted subcutaneously into immunodeficient mice (CB-17 SCID). We observed pulp-like tissues with odontoblasts capable of generating new tubular dentin throughout the root canals. Notably, the pulp tissue engineered with SHED injected with either Puramatrix™ or rhCollagen type I presented similar cellularity and vascularization when compared with control human dental pulps. Analysis of these data, collectively, demonstrates that SHED injected into full-length human root canals differentiate into functional odontoblasts, and suggests that such a strategy might facilitate the completion of root formation in necrotic immature permanent teeth.
Oral Sciences, Faculty of Dentistry, National University of Singapore, Singapore.
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Rosa
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Zhang
R H M
RH
Grande
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JE
Nör
D000293:Adolescent; D000818:Animals; D001641:Bicuspid / Q000033:anatomy & histology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D002648:Child; D024042:Collagen Type I / Q000737:chemistry; D003782:Dental Pulp / Q000033:anatomy & histology / Q000166:cytology* / Q000502:physiology; D003786:Dental Pulp Cavity / Q000033:anatomy & histology*; D003810:Dentinogenesis / Q000502:physiology; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D008297:Male; D051379:Mice; D016513:Mice, SCID; D018919:Neovascularization, Physiologic / Q000502:physiology; D009804:Odontoblasts / Q000502:physiology; D010455:Peptides / Q000737:chemistry; D011670:Pulpectomy; D011994:Recombinant Proteins; D013234:Stem Cells / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D014094:Tooth, Deciduous / Q000166:cytology
D024042:Collagen Type I; D010455:Peptides; C546681:RADA16-I; D011994:Recombinant Proteins
biocompatible materials; dentinogenesis; neovascularization; odontoblasts; stem cells; tissue scaffolds