Effect of Bothrops bilineata snake venom on neutrophil function.
The aim of the study was to evaluate the in vitro effects of Bothrops bilineata crude venom (BbV) on isolated human neutrophil function. We proved that BbV isn't toxic towards human neutrophils. During an incubation of human neutrophils with BbV hydrogen peroxide was produced. Moreover, BbV was able to stimulate neutrophil release of proinflammatory mediators such as IL-8 and IL-6 as well as PGE2 and NETs liberation. There is no data in the literature showing the effect of BbV on the production of IL-6 and IL-8 or NETs liberation by isolated human neutrophils. Taken together our results testify that BbV triggers relevant proinflammatory events in human neutrophils.
Laboratório de Imunologia Celular Aplicada à Saúde, Instituto de Pesquisas em Patologias Tropicais, IPEPATRO/FIOCRUZ Rondonia, Porto Velho, RO, Brazil.
Sulamita da Silva
Sda S
Setubal
Adriana Silva
AS
Pontes
Neriane Monteiro
NM
Nery
Jéssica Silva Félix
JS
Bastos
Onassis Boeri
OB
Castro
Weverson Luciano
WL
Pires
Kayena Delaix
KD
Zaqueo
Leonardo de Azevedo
Lde A
Calderon
Rodrigo Guerino
RG
Stábeli
Andreimar Martins
AM
Soares
Juliana Pavan
JP
Zuliani
D000818:Animals; D017837:Bothrops; D003435:Crotalid Venoms / Q000633:toxicity*; D015232:Dinoprostone / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D018675:Toxicity Tests
D003435:Crotalid Venoms; D015850:Interleukin-6; D016209:Interleukin-8; D006861:Hydrogen Peroxide; D015232:Dinoprostone
Bothrops (Bothriopsis) bilineata venom; Cytokines; NETs; Neutrophils; Reactive oxygen species