New side-view imaging technique for observing posterior chamber structures during cataract surgery in porcine eyes.
BACKGROUND
To develop a side-view imaging technique for observing the dynamic behavior of posterior chamber structures (PCSs) in porcine eyes which mimics closed-eye cataract surgery in humans.


METHODS
Enucleated porcine eyes were placed into liquid nitrogen for 5 seconds and immediately bisected at about a 45-degree angle to the equatorial plane. The anterior portion was attached firmly to a glass slide with superglue and sprinkled with wheat flour. Phacoemulsification and aspiration (PEA) was performed as in humans on 10 consecutive porcine eyes. The movements of the PCSs were monitored through the glass slide with a high-resolution video camera set below the cut surface of the eye. The intraocular pressure (IOP) was monitored during the surgery. The highest IOP, operation time, and volume of irrigation fluid of 10 whole eyes were compared to that obtained from the bisected eyes glued to a glass slide. In a second set of experiments, the strength of the seal between the bisected eye and the glass slide was tested in three sets of eyes: 1) frozen eye fixed with superglue with wheat flour for 3 min; 2) frozen eye fixed with superglue for 3 min; and 3) non-frozen eye fixed with superglue for 30 min. The highest IOP that led to a disruption of the seal was compared among the three groups.


RESULTS
PEA was successfully performed on 9 of 10 (90%) eyes with the movements of the PCSs clearly observed. The average maximum intraocular pressure of the 9 bisected eyes was 55.8 ± 4.7 mmHg and that for the 10 unbisected eyes was 55.3 ± 5.0 mmHg (P = 0.650). The frozen eye fixed with superglue in combination with wheat flour (Group 1) had the strongest sealing strength with an average IOP at the breaking point of 117.3 ± 36.2 mmHg.


CONCLUSIONS
Our side-view imaging technique can be used to evaluate the changes of the PCSs during intraocular surgery and for surgical training of new residents.
Department of Ophthalmology, Medicine of Sensory Function, Ehime University Graduate School of Medicine, Shitsukawa, Toon, Ehime 791-0295, Japan. tasaka@m.ehime-u.ac.jp.
Yoshitaka
Y
Tasaka
Noriyoshi
N
Minami
Takashi
T
Suzuki
Shiro
S
Kawasaki
Xiaodong
X
Zheng
Atsushi
A
Shiraishi
Toshihiko
T
Uno
Kensaku
K
Miyake
Yuichi
Y
Ohashi
D000818:Animals; D015925:Cryopreservation / Q000379:methods; D015353:Eye Enucleation; D007429:Intraocular Pressure / Q000502:physiology*; D023421:Models, Animal; D018918:Phacoemulsification / Q000009:adverse effects / Q000379:methods*; D057972:Posterior Eye Segment / Q000502:physiology*; D013552:Swine; D014741:Video Recording / Q000379:methods*