A simplified positive-sense-RNA virus construction approach that enhances analysis throughput.
Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a mutant library directly from a virus pool. The versatility of the approach described here expands the applicability of diverse genetic approaches to study these viruses.
National Center For Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathumthani, Thailand.
Bunpote
B
Siridechadilok
Methee
M
Gomutsukhavadee
Thunyarat
T
Sawaengpol
Sutha
S
Sangiambut
Chunya
C
Puttikhunt
Kwanrutai
K
Chin-inmanu
Prapat
P
Suriyaphol
Prida
P
Malasit
Gavin
G
Screaton
Juthathip
J
Mongkolsapaya
D015723:Gene Library; D005818:Genetic Engineering / Q000379:methods*; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*
D012367:RNA, Viral