Isolation of a novel microalgae strain Desmodesmus sp. and optimization of environmental factors for its biomass production.
A novel strain of unicellular green algae was isolated from fresh water samples collected from Yesanpo National Geopark, Laishui County of Hebei Province, China. The morphological and genomic identification of this strain was carried out using 18s rRNA analysis. This novel strain was identified as Desmodesmus sp. named as EJ15-2. Environmental factors for biomass production of Desmodesmus sp. EJ15-2 grown under autotrophic condition (BG11 medium) was optimized using response surface methodology (RSM). A high correlation coefficient (R(2)=0.923, p ≤ 0.01) indicated the adaptability of the second-order equation matched well with the growth condition of this strain. The optimal conditions for a relatively high biomass production (up to 0.758 g/L) were at 30°C, 98 μmol/m(2)/s and 14:10 (L:D), respectively.
College of Engineering/Biomass Engineering Center, China Agricultural University, PR China; Key Laboratory of Clean Production and Utilization of Renewable Energy, Ministry of Agriculture, PR China.
Fang
F
Ji
Rui
R
Hao
Ying
Y
Liu
Gang
G
Li
Yuguang
Y
Zhou
Renjie
R
Dong
D000704:Analysis of Variance; D018533:Biomass; D004777:Environment; D058086:Microalgae / Q000302:isolation & purification* / Q000378:metabolism*; D010802:Phylogeny; D012044:Regression Analysis
Biomass production; Desmodesmus sp.; Environmental factors; Response surface methodology