Fast assembly of non-thiolated DNA on gold surface at lower pH.
In a typical protocol for attaching DNA to a gold electrode, thiolated DNA is incubated with the electrode at neutral pH overnight. Here we report fast adsorption of non-thiolated DNA oligomers on gold electrodes at acidic pH (i.e., pH ~3.0). The peak-to-peak potential difference and the redox peak currents in typical cyclic voltammetry of [Fe(CN)6](3-) are investigated to monitor the attachment. Compared with incubation at neutral pH, the lower pH can significantly promote the adsorption processes, enabling efficient adsorption even in 30 min. The adsorption rate is DNA concentration-dependent, while the ionic strength shows no influence. Moreover, the adsorption is base-discriminative, with a preferred order of A>C>>G, T, which is attributed to the protonation of A and C at low pH and their higher binding affinity to gold surface. The immobilized DNA is functional and can hybridize with its complementary DNA but not a random DNA. This work is promising to provide a useful time-saving strategy for DNA assembly on gold electrodes, allowing fast fabrication of DNA-based biosensors and devices.
State Key Laboratory of Bioelectronics, Southeast University, 2 Sipailou, Nanjing 210096, PR China.
Hui
H
Jiang
Elsa M
EM
Materon
Maria Del Pilar Taboada
Mdel P
Sotomayor
Juewen
J
Liu
D000327:Adsorption; D004247:DNA / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009693:Nucleic Acid Hybridization; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013499:Surface Properties
D013438:Sulfhydryl Compounds; D006046:Gold; D004247:DNA
Adsorption; Biosensors; DNA; Gold; Immobilization