Development and application of recombinant antibody-based immunoassays to tetraconazole residue analysis in fruit juices.
Tetraconazole is currently used as a fungicide in fruit and vegetables. The aim of this work was the development of immunochemical techniques based on recombinant antibodies for the screening of tetraconazole residues in fruit juices. Recombinant antibodies were produced from a hybridoma cell line secreting a monoclonal antibody specific for tetraconazole and from lymphocytes of mice hyperimmunised with tetraconazole haptens conjugated to bovine serum albumin. From these antibodies, enzyme-linked immunosorbent assays in the conjugate-coated format were developed, which were able to detect tetraconazole standards down to 1ng/mL. From recovery studies with spiked samples, these immunoassays determined tetraconazole in orange and apple juices with acceptable reproducibility (coefficients of variation below 25%) and recoveries (ranging from 78% to 145%) for a screening technique. The analytical performance of RAb-based immunoassays was fairly similar to that of the MAb-based immunoassays. Due to their simplicity and high sample throughput, the developed recombinant-based immunoassays can be valuable analytical tools for the screening of tetraconazole residues in fruit juices at regulatory levels.
Instituto Interuniversitario de Investigación en Bioingeniería y Tecnología Orientada al Ser Humano, Universitat Politècnica de València, Camino de Vera s/n, Edificio 8B-N, 46022 Valencia, Spain.
Emma
E
Plana
Maria-José
MJ
Moreno
Ángel
Á
Montoya
Juan J
JJ
Manclús
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000235:genetics / Q000276:immunology; D001628:Beverages / Q000032:analysis*; D002722:Chlorobenzenes / Q000032:analysis*; D032084:Citrus sinensis / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D005638:Fruit / Q000737:chemistry; D005659:Fungicides, Industrial / Q000032:analysis*; D027845:Malus / Q000737:chemistry*; D051379:Mice; D010573:Pesticide Residues / Q000032:analysis*; D014230:Triazoles / Q000032:analysis*; D005466:Fluorocarbons
D000911:Antibodies, Monoclonal; D002722:Chlorobenzenes; D005659:Fungicides, Industrial; D010573:Pesticide Residues; D014230:Triazoles; C524826:tetraconazole; D005466:Fluorocarbons
ELISA; Fruit juices; Fungicide residues; Immunochemical analysis; Recombinant antibodies; ScFv; Tetraconazole