[Regulation of isoprenoid pathway for enhanced production of linalool in Saccharomyces cerevisiae].
Linalool is an important monoterpene, and widely used in food, pharmaceutical and cosmetic industry. The low concentration in plants and the difficulties in extraction restrict its large scale production. Saccharomyces cerevisiae can provide the monoterpene precursor, geranyl diphosphate (GPP) through its endogenous isoprenoid pathway. Therefore, it could be used as the host for monoterpene production. However, the weak metabolic flux through the isoprenoid pathway leads to the insufficient supply of GPP, and results in low monoterpene productivity. In order to increase the metabolic flux, we constructed the integrated expression plasmid pRS305-tHMG1 and free expression plasmid pYLIS-IDI1 to enhance the expression levels of isopentenyl diphosphate isomerase (IDI1) and a truncated 3-hydroxyl-3-methylglutaryl-CoA reductase gene (tHMG1). The two plasmids were separately transformed into S. cerevisiae CEN.PK2-1C, resulting in strains LS01 and LS02. The plasmid pYLIS-IDI1 was further transformed into strain LS01, resulting in strain LS03. GC-MS analysis showed that the linalool concentration was increased by 1.3 times and reached (127.71 +/- 7.68) microg/L. In conclusion, enhancement of the supply of GPP precursors through the regulation of isoprenoid pathway could increase the linalool production in S. cerevisiae.
Key Laboratory of Industrical Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
Mingxue
M
Sun
Jidong
J
Liu
Guocheng
G
Du
Jingwen
J
Zhou
Jian
J
Chen
D000080462:Acyclic Monoterpenes; D053898:Biosynthetic Pathways / Q000235:genetics*; D002070:Butadienes / Q000378:metabolism*; D045782:Hemiterpenes / Q000378:metabolism*; D039821:Monoterpenes / Q000378:metabolism*; D010420:Pentanes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*
D000080462:Acyclic Monoterpenes; D002070:Butadienes; D045782:Hemiterpenes; D039821:Monoterpenes; D010420:Pentanes; C005059:isoprene; C018584:linalool