Comparative metabolomics of muscle interstitium fluid in human trapezius myalgia: an in vivo microdialysis study.
OBJECTIVE
The mechanisms behind trapezius myalgia are unclear. Many hypotheses have been presented suggesting an altered metabolism in the muscle. Here, muscle microdialysate from healthy and myalgic muscle is analysed using metabolomics. Metabolomics analyse a vast number of metabolites, enabling a comprehensive explorative screening of the cellular processes in the muscle.


METHODS
Microdialysate samples were obtained from the shoulder muscle of healthy and myalgic subjects that performed a work and stress test. Samples from the baseline period and from the recovery period were analysed using gas chromatography—mass spectrometry (GC–MS) together with multivariate analysis to detect differences in extracellular content of metabolites between groups. Systematic differences in metabolites between groups were identified using multivariate analysis and orthogonal partial least square discriminate analysis (OPLS-DA). A complementary Mann–Whitney U test of group difference in individual metabolites was also performed.


RESULTS
A large number of metabolites were detected and identified in this screening study. At baseline, no systematic differences between groups were observed according to the OPLS-DA. However, two metabolites, l-leucine and pyroglutamic acid, were significantly more abundant in the myalgic muscle compared to the healthy muscle. In the recovery period, systematic difference in metabolites between the groups was observed according to the OPLS-DA. The groups differed in amino acids, fatty acids and carbohydrates. Myristic acid and putrescine were significantly more abundant and beta-d-glucopyranose was significantly less abundant in the myalgic muscle.


CONCLUSIONS
This study provides important information regarding the metabolite content, thereby presenting new clues regarding the pathophysiology of the myalgic muscle.
J
J
Hadrévi
B
B
Ghafouri
A
A
Sjörs
H
H
Antti
B
B
Larsson
A G
AG
Crenshaw
B
B
Gerdle
F
F
Hellström
D000328:Adult; D016022:Case-Control Studies; D015444:Exercise; D045604:Extracellular Fluid / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D006801:Humans; D007930:Leucine / Q000378:metabolism; D055442:Metabolome; D017551:Microdialysis; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D063806:Myalgia / Q000378:metabolism*; D019814:Myristic Acid; D011700:Putrescine / Q000378:metabolism; D011761:Pyrrolidonecarboxylic Acid / Q000378:metabolism
D005227:Fatty Acids; D019814:Myristic Acid; D007930:Leucine; D011761:Pyrrolidonecarboxylic Acid; D011700:Putrescine