Phage display as a technology delivering on the promise of peptide drug discovery.
Phage display represents an important approach in the development pipeline for producing peptides and peptidomimetics therapeutics. Using randomly generated DNA sequences and molecular biology techniques, large diverse peptide libraries can be displayed on the phage surface. The phage library can be incubated with a target of interest and the phage which bind can be isolated and sequenced to reveal the displayed peptides' primary structure. In this review, we focus on the 'mechanics' of the phage display process, whilst highlighting many diverse and subtle ways it has been used to further the drug-development process, including the potential for the phage particle itself to be used as a drug carrier targeted to a particular pathogen or cell type in the body.
Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Maryam
M
Hamzeh-Mivehroud
Ali Akbar
AA
Alizadeh
Michael B
MB
Morris
W Bret
WB
Church
Siavoush
S
Dastmalchi
D000818:Animals; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004337:Drug Carriers / Q000737:chemistry; D015195:Drug Design; D055808:Drug Discovery / Q000379:methods*; D006801:Humans; D008967:Molecular Biology / Q000379:methods; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000235:genetics
D004337:Drug Carriers; D019151:Peptide Library; D010455:Peptides