The investigation of the interaction between Tropicamide and bovine serum albumin by spectroscopic methods.
The fluorescence and ultraviolet-visible (UV-Vis) spectroscopy were explored to study the interaction between Tropicamide (TA) and bovine serum albumin (BSA) at three different temperatures (292, 301 and 310K) under imitated physiological conditions. The experimental results showed that the fluorescence quenching mechanism between TA and BSA was static quenching procedure. The binding constant (Ka), binding sites (n) were obtained. The corresponding thermodynamic parameters (ΔH, ΔS and ΔG) of the interaction system were calculated at different temperatures. The results revealed that the binding process is spontaneous, hydrogen binds and vander Waals were the main force to stabilize the complex. According to Förster non-radiation energy transfer theory, the binding distance between TA and BSA was calculated to be 4.90 nm. Synchronous fluorescence spectroscopy indicated the conformation of BSA changed in the presence of TA. Furthermore, the effect of some common metal ions (Mg(2+), Ca(2+), Cu(2+), and Ni(2+)) on the binding constants between TA and BSA were examined.
Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China; State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen 361005, China. Electronic address: yu_xianyong@163.com.
Xianyong
X
Yu
Zhixi
Z
Liao
Qing
Q
Yao
Heting
H
Liu
Xiaofang
X
Li
Pinggui
P
Yi
D000818:Animals; D002417:Cattle; D004735:Energy Transfer; D007477:Ions; D008968:Molecular Conformation; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D014331:Tropicamide / Q000737:chemistry / Q000378:metabolism*
D007477:Ions; D012710:Serum Albumin, Bovine; D014331:Tropicamide
Bovine serum albumin; Fluorescence spectroscopy; Interaction; Tropicamide; Ultraviolet–visible spectroscopy