[Analysis of the protein expression and gene amplification of HER2 in gastric cancer].
OBJECTIVE
To explore the clinical significance of protein expression and gene amplification of HER2 in gastric cancer.


METHODS
Immunohistochemistry (IHC) and chromogenic in situ hybridization (CISH) method were used to detect protein expression and gene amplification of HER2 in 80 specimens of gastric cancer patients.


RESULTS
Protein expression of HER2 was negative in 51 cases, (+) in 12 cases, (++) in 12 cases, (+++) in 5 cases, and the positive expression rate was 21.3% (17/80). Seven (8.8%) cases had gene amplification of HER2, including gene critical amplification in 3 (3.8%) cases. The result of IHC was positively correlated with CISH (P<0.05), and the coincidence rate was 85.0% (68/80). HER2 positive expression rate was higher in the gastroesophageal junction carcinoma, poorly differentiated and stage III-IIII gastric cancer (all P<0.05).


CONCLUSIONS
The gastric cancer tissue has high positive rate of protein expression and gene amplification of HER2, which is closely correlated to the development of gastric cancer.
Department of General Surgery, Huangdao Branch, Affiliated Hospital, Qingdao University Medical College, Qingdao 266555, China. cloudylucn@126.com.
Jun-lin
JL
Liu
Zhen-kai
ZK
Li
Fang-jie
FJ
Xin
Han
H
Zhao
Wei
W
Zhao
Yuan
Y
Gao
Ji-lin
JL
Hu
Xian-xiang
XX
Zhang
Mao-shen
MS
Zhang
Bing-yuan
BY
Zhang
Yun
Y
Lu
D000328:Adult; D000368:Aged; D005260:Female; D005784:Gene Amplification; D006801:Humans; D008297:Male; D008875:Middle Aged; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human