Erythropoietin activates Keap1-Nrf2/ARE pathway in rat brain after ischemia.
rhEPO has previously been shown to exert neuroprotective action in focal cerebral ischemia. However, its mechanism is not clear. We established the model of permanent focal cerebral ischemia. rhEPO was administered (5000 IU/kg i.p.) 2 h later after the successful ischemia model in rhEPO group and increased translation of Nrf2 and HO-1 and decreased the H2O2 concentration in the brain confirming activation of the Keap1-Nrf2/ARE pathway. The results show that rhEPO activate Keap1-Nrf2/ARE pathway after ischemia to protect the brain tissue.
Department of Neurology, The First Hospital of Shanxi Medical University , Shanxi , China.
Huaxing
H
Meng
Junhong
J
Guo
Huifang
H
Wang
Peng
P
Yan
Xiaoyuan
X
Niu
Jin
J
Zhang
D000818:Animals; D008297:Male; D051381:Rats; D001834:Body Water / Q000378:metabolism; D001923:Brain Chemistry / Q000187:drug effects; D002545:Brain Ischemia / Q000188:drug therapy / Q000378:metabolism; D002544:Cerebral Infarction / Q000188:drug therapy / Q000378:metabolism; D004921:Erythropoietin / Q000494:pharmacology / Q000627:therapeutic use; D051547:Heme Oxygenase-1 / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D000072019:Kelch-Like ECH-Associated Protein 1; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000378:metabolism
D004921:Erythropoietin; D051547:Heme Oxygenase-1; D006861:Hydrogen Peroxide; D047908:Intracellular Signaling Peptides and Proteins; C486610:KEAP1 protein, rat; D000072019:Kelch-Like ECH-Associated Protein 1; D011994:Recombinant Proteins; D051267:NF-E2-Related Factor 2