Fast, label-free force spectroscopy of histone-DNA interactions in individual nucleosomes using nanopores.
Herein we report a novel approach for fast, label-free probing of DNA-histone interactions in individual nucleosomes. We use solid-state nanopores to unravel individual DNA/histone complexes for the first time and find that the unraveling time depends on the applied electrophoretic force, and our results are in line with previous studies that employ optical tweezers. Our approach for studying nucleosomal interactions can greatly accelerate the understanding of fundamental mechanisms by which transcription, replication, and repair processes in a cell are modulated through DNA-histone interactions, as well as in diagnosis of diseases with abnormal patterns of DNA and histone modifications.
Departments of Physics and Chemistry/Chemical Biology, Northeastern University , Boston, Massachusetts 02115, United States.
Andrey
A
Ivankin
Spencer
S
Carson
Shannon R M
SR
Kinney
Meni
M
Wanunu
D004247:DNA / Q000737:chemistry*; D006657:Histones / Q000737:chemistry*; D008958:Models, Molecular; D058608:Nanopores; D009707:Nucleosomes / Q000737:chemistry*; D010316:Particle Size; D013057:Spectrum Analysis / Q000379:methods*; D013499:Surface Properties; D013997:Time Factors
D006657:Histones; D009707:Nucleosomes; D004247:DNA