Characterization of the α- and β-mannosidases of Porphyromonas gingivalis.
Mannose is an important sugar in the biology of the Gram-negative bacterium Porphyromonas gingivalis. It is a major component of the oligosaccharides attached to the Arg-gingipain cysteine proteases, the repeating units of an acidic lipopolysaccharide (A-LPS), and the core regions of both types of LPS produced by the organism (O-LPS and A-LPS) and a reported extracellular polysaccharide (EPS) isolated from spent culture medium. The organism occurs at inflamed sites in periodontal tissues, where it is exposed to host glycoproteins rich in mannose, which may be substrates for the acquisition of mannose by P. gingivalis. Five potential mannosidases were identified in the P. gingivalis W83 genome that may play a role in mannose acquisition. Four mannosidases were characterized in this study: PG0032 was a β-mannosidase, whereas PG0902 and PG1712 were capable of hydrolyzing p-nitrophenyl α-d-mannopyranoside. PG1711 and PG1712 were α-1 → 3 and α-1 → 2 mannosidases, respectively. No enzyme function could be assigned to PG0973. α-1 → 6 mannobiose was not hydrolyzed by P. gingivalis W50. EPS present in the culture supernatant was shown to be identical to yeast mannan and a component of the medium used for culturing P. gingivalis and was resistant to hydrolysis by mannosidases. Synthesis of O-LPS and A-LPS and glycosylation of the gingipains appeared to be unaffected in all mutants. Thus, α- and β-mannosidases of P. gingivalis are not involved in the harnessing of mannan/mannose from the growth medium for these biosynthetic processes. P. gingivalis grown in chemically defined medium devoid of carbohydrate showed reduced α-mannosidase activity (25%), suggesting these enzymes are environmentally regulated.
Queen Mary University of London, Barts, and The London School of Medicine and Dentistry, Centre for Immunology and Infectious Disease, Blizard Institute, London, United Kingdom.
Minnie
M
Rangarajan
Joseph
J
Aduse-Opoku
Ahmed
A
Hashim
Nikolay
N
Paramonov
Michael A
MA
Curtis
D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D000080867:Gingipain Cysteine Endopeptidases; D009682:Magnetic Resonance Spectroscopy; D009154:Mutation; D016966:Porphyromonas gingivalis / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D043323:alpha-Mannosidase / Q000235:genetics / Q000378:metabolism*; D044902:beta-Mannosidase / Q000235:genetics / Q000378:metabolism*
D018829:Adhesins, Bacterial; D000080867:Gingipain Cysteine Endopeptidases; D043323:alpha-Mannosidase; D044902:beta-Mannosidase; D003546:Cysteine Endopeptidases