Case report: the use of annexin V coupled with magnetic activated cell sorting in cryopreserved spermatozoa from a male cancer survivor: healthy twin newborns after two previous ICSI failures.
OBJECTIVE
The aim of the study is to report successful outcome (live births) after sperm sorting with annexin V-MACS on cryopreserved spermatozoa with high level of sperm DNA fragmentation from a cancer patient survivor.


METHODS
Cryopreserved spermatozoa were sorted with annexin V-MACS prior to ICSI. Sperm DNA fragmentation was evaluated by SCSA(®) and TUNEL.


RESULTS
The couple had two previous IVF/ICSI cycles failures using sperm cryopreserved before cancer treatment. On third ICSI cycle attempt results were as follow: pre-annexin V-MACS sperm quality: 10 × 10(6)/ml, 3.3 % progressive motility, 1 % normal forms, TUNEL: 72.5 % positive cells, SCSA(®): 76.6 % DFI. Post-annexin V-MACS sperm quality: 2.8 × 10(6)/ml, 10 % progressive motility, TUNEL: 58.8 % positive cells. Eight metaphase II oocytes were collected, 4 fertilized, 2 embryos were transferred on day 3 and healthy twins were born (1 boy, 1 girl).


CONCLUSIONS
Annexin V-MACS technique could be a potential tool to improve sperm quality on cryopreserved spermatozoa of cancer patient and improve ICSI outcome.
MUHC Reproductive Center, McGill University Health Center, 687 Pine Av. West, Montreal, Quebec, Canada.
María Belén
MB
Herrero
Géraldine
G
Delbes
Jin-Tae
JT
Chung
Weon-Young
WY
Son
Hananel
H
Holzer
William
W
Buckett
Peter
P
Chan
D000328:Adult; D017304:Annexin A5 / Q000008:administration & dosage*; D002469:Cell Separation / Q000379:methods; D015925:Cryopreservation; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008228:Lymphoma, Non-Hodgkin; D008280:Magnetics; D008297:Male; D008863:Microspheres; D011247:Pregnancy; D011256:Pregnancy Outcome; D055101:Semen Analysis; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology* / Q000187:drug effects; D017741:Survivors; D014427:Twins
D017304:Annexin A5