Ursolic acid induces apoptosis of SW480 cells via p53 activation.
The purpose of this study is to assess the effect of ursolic acid, a triterpene, on induction of apoptosis in human colon adenocarcinoma cells, SW480. The cells treated with nontoxic concentration of ursolic acid showed the presence of apoptotic bodies and DNA fragmentation in a dose dependent manner. Both the expression level and transcriptional activity of p53, an apoptotic protein, were remarkably up-regulated in cells treated with ursolic acid compared to blank group. In addition, the expression levels of p-p53, acetyl-p53, NF-κB, p-p21, Bax, Caspase-3 and Caspase-9, apoptotic proteins, were increased in cells treated with ursolic acid compared to blank group. Above results demonstrate that ursolic acid induces the activation of p53, NF-κB and Bax, leading to the enhancement of p21 transcriptional activity and activation of Caspase-9 and -3, thus finally induces apoptosis of SW480 cells.
Department of Chemistry, Dong-Eui University, Busan 614-714, Republic of Korea.
Hyang
H
Nam
Moon-Moo
MM
Kim
D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics; D053938:DNA Fragmentation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D014315:Triterpenes / Q000494:pharmacology*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism; D000097245:Ursolic Acid
D053148:Caspase 3; D053453:Caspase 9; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2; D014315:Triterpenes; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; D000097245:Ursolic Acid; C494826:BAX protein, human; C493956:CDKN1A protein, human
Apoptosis; P-p53; SW480; Ursolic acid; p21