Microarray analysis of hepatic gene expression in juvenile Japanese flounder Paralichthys olivaceus fed diets supplemented with fish or vegetable oils.
Gene expression profiling was performed in Japanese flounder Paralichthys olivaceus fed diets supplemented with fish oil (FO), linseed oil (LO), or olive oil (OO) for 6 weeks. The LO and OO groups showed significantly retarded growth, lower feed intake, lower protein efficiency ratio, and lower hepatosomatic index (P < 0.05). Liver fatty acid composition reflected the dietary fatty acid composition. Microarray analysis revealed that dietary n - 3 highly unsaturated fatty acid (HUFA) deficiency affected 169 transcripts. In the LO group, 57 genes were up-regulated and 38 genes were down-regulated, whereas in the OO group nine genes were up-regulated and 87 genes were down-regulated. Analysis of the functional annotations suggested that dietary n - 3 HUFA affected genes involved in signal transduction (23.2 %), cellular processes (21.1 %), metabolism (including glucose, lipid, and nucleobase; 15.5 %), transport (11.3 %), regulation of transcription (10.5 %), and immune response (4.2 %). Several genes encoding serine/threonine kinases such as protein kinase C and calmodulin-dependent kinase and nuclear hormone receptors such as vitamin D receptor, retinoic acid receptor, and receptors for cytokines (bone morphogenic protein and transforming growth factor β) were affected. Among 169 transcripts, 22 genes were affected in both LO and OO groups. The present study identified several genes involved in n - 3 HUFA deficiency-sensitive pathways, which will be useful for selective breeding of flounder strains able to adapt to n - 3 HUFA deficiency.
Department of Marine Bioscience, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan, Minato 4-5-7, Tokyo, 108-8477, Japan.
Ubonrat
U
Limtipsuntorn
Yutaka
Y
Haga
Hidehiro
H
Kondo
Ikuo
I
Hirono
Shuichi
S
Satoh
D000818:Animals; D001947:Breeding / Q000379:methods; D019587:Dietary Supplements; D005227:Fatty Acids / Q000378:metabolism; D005395:Fish Oils / Q000494:pharmacology*; D005432:Flounder / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling / Q000662:veterinary; D005786:Gene Expression Regulation / Q000187:drug effects*; D008099:Liver / Q000378:metabolism*; D046228:Microarray Analysis / Q000662:veterinary; D058977:Molecular Sequence Annotation; D010938:Plant Oils / Q000494:pharmacology*
D005227:Fatty Acids; D005395:Fish Oils; D010938:Plant Oils