Ultrasound assisted dispersive liquid-liquid microextraction followed by injector port silylation: a novel method for rapid determination of quinine in urine by GC-MS.
BACKGROUND
Silylation is a widely used derivatization method for the analysis of polar analytes by GC-MS. Ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) is an ecofriendly, rapid and simple microextraction method. For the first time, a novel approach has been developed and applied for the analysis of quinine in urine by combining UA-DLLME with injection port silylation.


RESULTS
The LOD and LOQ were found to be 5.4 and 18 ng/ml. The intra- and inter-day precisions were less than 5 and 8%, respectively. Mean recoveries of quinine were found to be in the range of 87 to 96%.


CONCLUSIONS
Ultrasound-assisted dispersive liquid-liquid microextraction is rapid, simple and consumes less reagent for the analysis of polar analytes such as quinine.
Analytical Chemistry Section, CSIR-Indian Institute of Toxicology Research, M G Marg, Lucknow, 226 001, UP, India.
Rajeev
R
Jain
Mohana Krishna Reddy
MK
Mudiam
Ratnasekhar
R
Ch
Abhishek
A
Chauhan
Haider A
HA
Khan
Rc
R
Murthy
D000328:Adult; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D064368:Healthy Volunteers; D006801:Humans; D006863:Hydrogen-Ion Concentration; D059627:Liquid Phase Microextraction / Q000379:methods*; D009994:Osmolar Concentration; D011803:Quinine / Q000652:urine*; D012821:Silanes / Q000737:chemistry*; D013010:Sonication / Q000379:methods*; D013997:Time Factors
D012821:Silanes; D011803:Quinine