Effect of phytoestrogens on basal and GnRH-induced gonadotropin secretion.
Plant-derived estrogens (phytoestrogens, PEs), like endogenous estrogens, affect a diverse array of tissues, including the bone, uterus, mammary gland, and components of the neural and cardiovascular systems. We hypothesized that PEs act directly at pituitary loci to attenuate basal FSH secretion and increase gonadotrope sensitivity to GnRH. To examine the effect of PEs on basal secretion and total production of FSH, ovine pituitary cells were incubated with PEs for 48 h. Conditioned media and cell extract were collected and assayed for FSH. Estradiol (E₂) and some PEs significantly decreased basal secretion of FSH. The most potent PEs in this regard were coumestrol (CM), zearalenone (ZR), and genistein (GN). The specificity of PE-induced suppression of basal FSH was indicated by the absence of suppression in cells coincubated with PEs and an estrogen receptor (ER) blocker (ICI 182 780; ICI). Secretion of LH during stimulation by a GnRH agonist (GnRH-A) was used as a measure of gonadotrope responsiveness. Incubation of cells for 12 h with E₂, CM, ZR, GN, or daidzein (DZ) enhanced the magnitude and sensitivity of LH secretion during subsequent exposure to graded levels of a GnRH-A. The E₂- and PE-dependent augmentation of gonadotrope responsiveness was nearly fully blocked during coincubation with ICI. Collectively, these data demonstrate that selected PEs (CM, ZR, and GN), like E₂, decrease basal secretion of FSH, reduce total FSH production, and enhance GnRH-A-induced LH secretion in a manner that is dependent on the ER.
Department of Animal Science, University of California, Davis, One Shields Avenue, Davis, California 95616, USA.
Sergio A
SA
Arispe
Betty
B
Adams
Thomas E
TE
Adams
D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002478:Cells, Cultured; D003375:Coumestrol / Q000037:antagonists & inhibitors / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D004958:Estradiol / Q000737:chemistry / Q000378:metabolism; D004965:Estrogen Antagonists / Q000494:pharmacology; D037201:Follicle Stimulating Hormone, beta Subunit / Q000378:metabolism*; D005503:Food Additives / Q000737:chemistry / Q000378:metabolism; D019833:Genistein / Q000037:antagonists & inhibitors / Q000378:metabolism; D015292:Glycoprotein Hormones, alpha Subunit / Q000096:biosynthesis / Q000378:metabolism*; D052681:Gonadotrophs / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists / Q000378:metabolism*; D037101:Luteinizing Hormone, beta Subunit / Q000096:biosynthesis / Q000378:metabolism*; D008297:Male; D048789:Phytoestrogens / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000037:antagonists & inhibitors / Q000378:metabolism; D034561:Sheep, Domestic / Q000502:physiology*; D015854:Up-Regulation / Q000187:drug effects; D015025:Zearalenone / Q000037:antagonists & inhibitors / Q000378:metabolism
D004965:Estrogen Antagonists; D037201:Follicle Stimulating Hormone, beta Subunit; D005503:Food Additives; D015292:Glycoprotein Hormones, alpha Subunit; D037101:Luteinizing Hormone, beta Subunit; D048789:Phytoestrogens; D011960:Receptors, Estrogen; D007987:Gonadotropin-Releasing Hormone; D004958:Estradiol; D015025:Zearalenone; D019833:Genistein; D003375:Coumestrol
FSH; LH; cell culture; estradiol; estrogen receptor; ovine pituitary cells; phytoestrogens