Design and evaluation of an electrochemical immunosensor for measles serodiagnosis using measles-specific Immunoglobulin G antibodies.
The design of electrochemical immunosensors for the detection of measles-specific antibodies is reported. The measles-antigen modified surface was used as an antibody capture surface. The detection of measles-specific IgG antibodies was accomplished using the voltammetric method and horse-radish peroxidase (HRP) labeled secondary antibody (anti-IgG) as a detecting antibody. The potential applications of the designed immunosensor were evaluated in buffer and serum solutions. The immunosensor exhibited good linearity at concentrations less than 100 ng mL(-1) with R(2)=0.997 and the limit of detection of 6.60 ng mL(-1) at 3σ. The potential application of the immunosensor was evaluated in the deliberately infected human and newborn calf serum samples with measles-IgG antibody mimicking real-life samples. The designed electrochemical immunosensor could differentiate between infected and un-infected serum samples as higher catalytic currents were obtained for infected serum samples.
Nanotechnology Innovation Centre, Advanced Materials Division, Mintek, Private Bag X3015, Randburg 2125, South Africa; Nanotechnology Innovation Centre, Sensors, Chemistry Department, Rhodes University, P.O. Box 94, Grahamstown 6140, South Africa. Electronic address: philanim@mintek.co.za.
Philani
P
Mashazi
Sibulelo
S
Vilakazi
Tebello
T
Nyokong
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000737:chemistry* / Q000276:immunology; D015374:Biosensing Techniques; D002417:Cattle; D004867:Equipment Design; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D007118:Immunoassay; D007074:Immunoglobulin G / Q000097:blood*; D007231:Infant, Newborn; D057230:Limit of Detection; D008457:Measles / Q000097:blood* / Q000175:diagnosis / Q000276:immunology / Q000821:virology
D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; C048844:anti-IgG; D006735:Horseradish Peroxidase
Horse-radish peroxidase; Immunosensors; Labeled-detection; Measles-antigen; Measles-specific antibodies