Enhanced levels of plant cell cycle inhibitors hamper root-knot nematode-induced feeding site development.
Root-knot nematodes (RKN) are highly specialized, obligatory plant parasites. These animals reprogram root cells to form large, multinucleate, and metabolically active feeding cells (giant cells) that provide a continuous nutrient supply during 3-6 weeks of the nematode's life. The establishment and maintenance of physiologically fully functional giant cells are necessary for the survival of these nematodes. As such, giant cells may be useful targets for applying strategies to reduce damage caused by these nematodes, aiming the reduction of their reproduction. We have recently reported the involvement of cell cycle inhibitors of Arabidopsis, named Kip-Related Proteins (KRPs), on nematode feeding site ontogeny. Our results have demonstrated that this family of cell cycle inhibitors can be envisaged to efficiently disrupt giant cell development, based on previous reports which showed that alterations in KRP concentration levels can induce cell cycle transitions. Herein, we demonstrated that by overexpressing KRP genes, giant cells development is severely compromised as well as nematode reproduction. Thus, control of root-knot nematodes by modulating cell cycle-directed pathways through the enhancement of KRP protein levels may serve as an attractive strategy to limit damage caused by these plant parasites.
Lab. Nematologia/ICAAM; Instituto de Ciências Agrárias e Ambientais Mediterrânicas; Universidade de Évora; Évora, Portugal.
Paulo
P
Vieira
Institut National de la Recherche Agronomique; UMR 1355 ISA/Centre National de la Recherche Scientifique; UMR 7254 ISA/ Université de Nice-Sophia Antipolis; UMR ISA; Sophia-Antipolis, France.
Gilbert
G
Engler
Institut National de la Recherche Agronomique; UMR 1355 ISA/Centre National de la Recherche Scientifique; UMR 7254 ISA/ Université de Nice-Sophia Antipolis; UMR ISA; Sophia-Antipolis, France.
Janice
J
de Almeida Engler
D000818:Animals; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000469:parasitology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002453:Cell Cycle; D005247:Feeding Behavior; D018506:Gene Expression Regulation, Plant; D006790:Host-Parasite Interactions; D009348:Nematoda / Q000502:physiology*; D010935:Plant Diseases / Q000469:parasitology; D018517:Plant Roots / Q000235:genetics / Q000469:parasitology*; D010941:Plant Tumors / Q000235:genetics / Q000469:parasitology
D029681:Arabidopsis Proteins
Arabidopsis; KRPgenes; Meloidogyne incognita; cyclin dependent kinase; gall formation; giant cells; kip-related proteins