Blue light-mediated manipulation of transcription factor activity in vivo.
We developed a novel technique for manipulating the activity of transcription factors with blue light (termed "PICCORO") using the bacterial BLUF-type photoreceptor protein PixD. The chimeric dominant-negative T-box transcription factor No Tail formed heterologous complexes with a PixD decamer in a light-dependent manner, and these complexes affected transcription repressor activity. When applied to zebrafish embryos, PICCORO permitted regulation of the activity of the mutant No Tail in response to 472-nm light provided by a light-emitting diode.
Center for Biological Resources & Informatics, Tokyo Institute of Technology , Yokohama 226-8501, Japan.
Shinji
S
Masuda
Yuki
Y
Nakatani
Shukun
S
Ren
Mikiko
M
Tanaka
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004625:Embryo, Nonmammalian; D005326:Fetal Proteins; D018507:Gene Expression Regulation, Developmental / Q000528:radiation effects*; D008027:Light; D050506:Mutant Chimeric Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020283:Photoreceptors, Microbial / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D046939:Synechocystis / Q000737:chemistry / Q000378:metabolism; D020825:T-Box Domain Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000528:radiation effects*; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000098908:Brachyury Protein
D001426:Bacterial Proteins; D005326:Fetal Proteins; D050506:Mutant Chimeric Proteins; D020283:Photoreceptors, Microbial; D020825:T-Box Domain Proteins; D029961:Zebrafish Proteins; D000098908:Brachyury Protein