[Method for determining brodifacoum in workplace air by high-performance liquid chromatography].
OBJECTIVE
To establish a method for determining brodifacoum in workplace air by high-performance liquid chromatography (HPLC).


METHODS
Brodifacoum in workplace air was collected with a polytetrafluoroethylene filter and desorbed by mixed solution of methanol and dichloromethane (20:80, V:V), and was then separated using an ODS column and determined by an ultraviolet detector; retention time was used for identification, and peak area was used for quantification.


RESULTS
The concentration of brodifacoum showed a linear relationship with peak area within 0.2∼10.0 µg/ml; the elution efficiency was 91.6%∼95.1%; the detection limit was 0.08 µg/ml (injection volume: 20 µl eluate); the minimum detectable concentration was 0.000 67 mg/m(3) (calculated by 240 L air sample).


CONCLUSIONS
This HPLC method is convenient and simple for air collection and sample preparation and meets the methodological requirements. Therefore, this method can be used for the determination of brodifacoum in workplace air.
National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Zhao-hui
ZH
Fu
Jian-ning
JN
Xu
Wen-lan
WL
Yu
Quan-kai
QK
Wang
An-na
AN
Wang
Feng
F
Tan
D015110:4-Hydroxycoumarins / Q000032:analysis*; D000388:Air / Q000032:analysis; D000395:Air Pollutants, Occupational / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017132:Workplace
D015110:4-Hydroxycoumarins; D000395:Air Pollutants, Occupational; C013418:bromfenacoum