HindII and SduI digests of heat-shock protein 70 PCR for Leishmania typing.
Restriction fragment length polymorphisms of the heat-shock protein 70 gene have been used for discriminating Leishmania species. Here, we validated HindII as a much cheaper alternative to EcoRII and SduI for discriminating Leishmania (Viannia) braziliensis from Leishmania (Viannia) naiffi and an atypical Leishmania (V.) braziliensis group, which was previously not possible.
Departamento de Parasitología, Instituto de Medicina Tropical "Pedro Kourí", Havana, Cuba.
Jorge
J
Fraga
Ana M
AM
Montalvo
Llse
L
Maes
Jean-Claude
JC
Dujardin
Gert
G
Van der Auwera
D016054:DNA, Protozoan / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D007891:Leishmania / Q000145:classification* / Q000235:genetics*; D058889:Molecular Typing / Q000379:methods*; D010274:Parasitology / Q000379:methods*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015800:Protozoan Proteins / Q000235:genetics*
D016054:DNA, Protozoan; D018840:HSP70 Heat-Shock Proteins; D015800:Protozoan Proteins; D015252:Deoxyribonucleases, Type II Site-Specific; C033083:GDGCHC-specific GATC-specific type II deoxyribonucleases; C024400:GTYRAC-specific type II deoxyribonucleases
Leishmania; PCR-RFLP; Typing; hsp70