Pectic polysaccharides of the fresh plum Prunus domestica L. isolated with a simulated gastric fluid and their anti-inflammatory and antioxidant activities.
A pectic polysaccharide, designated as PD, was extracted from fresh plums (Prunus domestica L.) with a simulated gastric fluid. Galacturonan, which was partially substituted with methyl and O-acetyl ester groups, and rhamnogalacturonan were the main constituents of the linear regions of the sugar chains of PD. The ramified region contained mainly 1,4-linked β-d-galactopyranose residues and, to a lesser extent, 1,5-linked α-l-arabinofuranose residues. The separation of PD, by DEAE-cellulose column chromatography, yielded two pectic fractions: PD-1 and PD-2, eluted with 0.1 and 0.2 M NaCl, respectively. Enzymatic digestion of PD with 1,4-α-d-polygalacturonase yielded the fraction PD-E. The parent pectin PD and the PD-1 fraction were found to diminish the adhesion of peritoneal leukocytes at the concentrations of 0.05-1.0mg/ml. However, the PD-E fraction failed to have an effect on cell adhesion at the concentrations of 0.05-0.1mg/ml. PD, PD-1 and PD-E were found to inhibit the production of superoxide anion radicals by reducing xanthine oxidase activity by 38%, 97% and 47%, respectively. Therefore, the PD-1 fraction appeared to be an active fragment of pectic macromolecule isolated from fresh plum with a simulated gastric fluid.
Institute of Physiology, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, 50, Pervomaiskaya Str., Syktyvkar 167982, Russia. Electronic address: popov@physiol.komisc.ru.
Sergey V
SV
Popov
Raisa G
RG
Ovodova
Victoria V
VV
Golovchenko
Daria S
DS
Khramova
Pavel A
PA
Markov
Vasily V
VV
Smirnov
Alexandre S
AS
Shashkov
Yury S
YS
Ovodov
D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D005750:Gastric Juice / Q000737:chemistry*; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D010368:Pectins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D027861:Prunus / Q000737:chemistry*
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts; D010368:Pectins
Leukocyte adhesion; NMR spectroscopy of polysaccharides; Pectin structure; Plums; Simulated gastric fluid; Superoxide anion radical scavenging