Agrobacterium tumefaciens-mediated genetic transformation of the Taxol-producing endophytic fungus Ozonium sp EFY21.
An efficient Agrobacterium tumefaciens-mediated genetic transformation method was successfully established for a newly isolated Taxol-producing fungus, Ozonium sp EFY21. A specific hygromycin B resistance expression vector, pCAMBIA1304'AN7-1, was constructed for fungal transformation. Key factors affecting transformation efficiency were thoroughly investigated and optimized. PCR amplification and Southern hybridization were used to verify the transformation events. This study should pave the way for future genetic modification studies of Ozonium sp EFY21.
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China.
L
L
Liu
Y M
YM
Wei
X W
XW
Zhou
J
J
Lin
X F
XF
Sun
K X
KX
Tang
D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism*; D001203:Ascomycota / Q000235:genetics / Q000378:metabolism*; D060026:Endophytes / Q000235:genetics / Q000378:metabolism*; D017239:Paclitaxel / Q000096:biosynthesis*; D011523:Protoplasts / Q000378:metabolism; D014170:Transformation, Genetic
D017239:Paclitaxel