[Research on the effect of extract of dentin matrix on the differentiation of dental pulp cells].
OBJECTIVE
To investigate the effects of human treated dentin matrix (hTDM) extracellular matrix molecules on odontogenetic and neural differentiation of human dental pulp cells (hDPCs) with an aim to find an effective method to collect extracellular matrix molecules to contribute to reparation dental-pulp complex with dentin defects.


METHODS
hDPCs were obtained and biological characteristics such as source of cells and multi-differentiation potentials were assessed using immunofluorescence and flow cytometry. Fabrication of hTDM extracts and hDPCs was induced with it for 1 week. The odontogenetic differentiation associated genes were tested by qRT-PCR. Results qRT-PCR results showed that cells were higher expression of odontogenetic differentiation associated genes ALP, OPN, OCN, BSP, DMP-1, DSP, beta-III tubulin.


CONCLUSIONS
The method of extracting extracellular matrix molecules from dentin matrix was effective. The extract liquid provides a suitable microenvironment for odontogenetic and neural differentiation of hDPCs and contributes to reparation dental-pulp complex.
Regenerative Medicine Research Center, West China Hospital, Sichuan. University, Chengdu 610041, China.
Cai-yun
CY
Cui
Yang
Y
Bo
Gang
G
Chen
Lei
L
Sheng
Jian-Ping
JP
Zhao
Xiang-Chen
XC
Qiao
Wei-Hua
WH
Guo
Wei-Dong
WD
Tian
D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D003804:Dentin / Q000737:chemistry* / Q000502:physiology; D005109:Extracellular Matrix / Q000737:chemistry*; D006801:Humans; D009474:Neurons / Q000166:cytology; D009804:Odontoblasts / Q000166:cytology