Ossification of the posterior longitudinal ligament related genes identification using microarray gene expression profiling and bioinformatics analysis.
Ossification of the posterior longitudinal ligament (OPLL) is a kind of disease with physical barriers and neurological disorders. The objective of this study was to explore the differentially expressed genes (DEGs) in OPLL patient ligament cells and identify the target sites for the prevention and treatment of OPLL in clinic. Gene expression data GSE5464 was downloaded from Gene Expression Omnibus; then DEGs were screened by limma package in R language, and changed functions and pathways of OPLL cells compared to normal cells were identified by DAVID (The Database for Annotation, Visualization and Integrated Discovery); finally, an interaction network of DEGs was constructed by string. A total of 1536 DEGs were screened, with 31 down-regulated and 1505 up-regulated genes. Response to wounding function and Toll-like receptor signaling pathway may involve in the development of OPLL. Genes, such as PDGFB, PRDX2 may involve in OPLL through response to wounding function. Toll-like receptor signaling pathway enriched genes such as TLR1, TLR5, and TLR7 may involve in spine cord injury in OPLL. PIK3R1 was the hub gene in the network of DEGs with the highest degree; INSR was one of the most closely related genes of it. OPLL related genes screened by microarray gene expression profiling and bioinformatics analysis may be helpful for elucidating the mechanism of OPLL.
Department of Orthopaedics, Changzheng Hospital, The Second Military Medical University, No. 415, Fengyang Road, Huangpu District, Shanghai 200003, China. Electronic address: hailonghe@hotmail.com.
Hailong
H
He
Lingzhou
L
Mao
Peng
P
Xu
Yanhai
Y
Xi
Ning
N
Xu
Mingtao
M
Xue
Jiangming
J
Yu
Xiaojian
X
Ye
D016000:Cluster Analysis; D019295:Computational Biology; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D056726:Genetic Association Studies; D006801:Humans; D053858:Metabolic Networks and Pathways / Q000235:genetics; D046228:Microarray Analysis; D017887:Ossification of Posterior Longitudinal Ligament / Q000235:genetics*
BH; BMP-2; Benjamin and Hochberg; DAVID; DEGs; Differentially expressed gene; Dysfunctional pathway; ESR2; FC; FDR; GO; Gene Ontology; INSR; KEGG; Kyoto Encyclopedia Genes and Genomes; MAPK10; OPLL; Ossification of the posterior longitudinal ligament; PDGFB; PI3K; PIK3R1; PRDX2; Phosphoinositide 3-kinase; TLR 1; TLRs; The Database for Annotation, Visualization and Integrated Discovery; Toll-like receptor 1; Toll-like receptors; bone morphogenetic protein 2; differentially expressed genes; estrogen receptor 2; false discovery rate; fold chance; insulin receptor; mitogen-activated protein kinases 10; ossification of the posterior longitudinal ligament; peroxiredoxin 2; phosphoinositide-3-kinase, regulatory subunit 1; platelet-derived growth factor-B