Mutational analysis of the Verticillium dahliae protein elicitor PevD1 identifies distinctive regions responsible for hypersensitive response and systemic acquired resistance in tobacco.
In our previous study, PevD1 was characterized as a novel protein elicitor produced by Verticillium dahliae inducing hypersensitive response (HR) and systemic acquired resistance (SAR) in tobacco plants; however, the detailed mechanisms of PevD1's elicitor activity remain unclear. In this study, five mutant fragments of PevD1 were generated by polymerase chain reaction-based mutagenesis and the truncated proteins expressed in Escherichia coli were used to test their elicitor activities. Biological activity analysis showed that the N-terminal and C-terminal of PevD1 had distinct influence on HR and SAR elicitation. Fragment PevD1ΔN98, which spans the C-terminal 57 amino acids of PevD1, was critical for the induction of HR in tobacco plants. In contrast, fragment PevD1ΔC57, the N-terminal of 98 amino acids of PevD1, retained the ability to induce SAR against tobacco mosaic virus (TMV) but not induction of HR, suggesting that the induction of HR is not essential for SAR mediated by PevD1. Our results indicated that fragment PevD1ΔC57 could be a candidate peptide for plant protection against pathogens without causing negative effects.
State Key Laboratory of Grassland Agro-Ecosystems, School of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, PR China; Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 12, Zhongguancun South Street, Beijing 100081, PR China.
Wenxian
W
Liu
Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 12, Zhongguancun South Street, Beijing 100081, PR China. Electronic address: zenghongmei@caas.cn.
Hongmei
H
Zeng
State Key Laboratory of Grassland Agro-Ecosystems, School of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, PR China.
Zhipeng
Z
Liu
Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 12, Zhongguancun South Street, Beijing 100081, PR China.
Xiufen
X
Yang
Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 12, Zhongguancun South Street, Beijing 100081, PR China.
Lihua
L
Guo
Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 12, Zhongguancun South Street, Beijing 100081, PR China. Electronic address: qiudewen@caas.cn.
Dewen
D
Qiu
D004252:DNA Mutational Analysis; D060467:Disease Resistance; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000276:immunology*; D050505:Mutant Proteins / Q000235:genetics / Q000276:immunology; D010935:Plant Diseases / Q000276:immunology* / Q000821:virology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D014026:Nicotiana / Q000276:immunology* / Q000382:microbiology; D014027:Tobacco Mosaic Virus / Q000276:immunology*; D020172:Verticillium / Q000235:genetics / Q000276:immunology*
D005656:Fungal Proteins; D050505:Mutant Proteins; D011994:Recombinant Proteins
Elicitor; Hypersensitive response; PevD1; Systemic acquired resistance; Verticillium dahliae