Pathogenic analysis of Vibrio alginolyticus infection in a mouse model.
Vibrio alginolyticus is a Gram-negative halophilic bacterium and has been recognized as an opportunistic pathogen to both humans and marine animals. So far, most studies have been focused on marine animals and few reports have been aimed at mammals, including human. In this study, we first established a mouse model to understand the pathogenic mechanism of V. alginolyticus infection. After infection via intraperitoneal injection, hematological and liver function indicators were evaluated and serum interleukin (IL)-1β and IL-6 expression were detected by ELISA. Furthermore, we compared the virulence of two V. alginolyticus strains, ATCC17749T and E0666. The results demonstrated that V. alginolyticus infection causes robust lung and liver damage and induces changes in IL-1β, IL-6, hematological, and liver indicators. In addition, the ATCC17749T strain appeared to be more virulent than the E0666 strain. Better understanding of the pathogenic mechanism of V. alginolyticus infection should guide effective prevention and therapy for V. alginolyticus infection.
Department of Laboratory Medicine, General Hospital of Jinan Military Region of PLA, Jinan, 250031, Shandong Province, People's Republic of China.
Xiao-Fei
XF
Liu
Helin
H
Zhang
Xingshan
X
Liu
Yanwen
Y
Gong
Yingjian
Y
Chen
Yuan
Y
Cao
Chengjin
C
Hu
D000818:Animals; D001774:Blood Chemical Analysis; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D004798:Enzymes / Q000097:blood; D005260:Female; D006651:Histocytochemistry; D008099:Liver / Q000473:pathology; D008111:Liver Function Tests; D008168:Lung / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008853:Microscopy; D014735:Vibrio Infections / Q000382:microbiology* / Q000473:pathology*; D044149:Vibrio alginolyticus / Q000472:pathogenicity*; D014774:Virulence
D016207:Cytokines; D004798:Enzymes