An eco-friendly, simple, and sensitive fluorescence biosensor for the detection of choline and acetylcholine based on C-dots and the Fenton reaction.
A simple and novel method is proposed for the preparation of Carbon dots (C-dots) with excellent properties. We firstly demonstrated that the fluorescence of C-dots decreased apparently in the presence of H2O2 and Fe(2+). Based on the this finding, C-dots are successfully adopted as probes for the detection of H2O2. After the experimental conditions are optimized, the limit of detection (LOD) for H2O2 is found to be 0.1 μM. Furthermore, we established an eco-friendly, simple and sensitive biosensor for the detection of choline and acetylcholine (ACh) based on the detection of H2O2 using C-dots as probes. The detection limit for choline is 0.1 μM and the linear range is 0.1-40 μM. The detection limit for ACh is found to be 0.5 μM and the linear range is 0.5-60 μM. The excellent performance of the proposed biosensor shows that this method possesses the potential for practical application.
Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, No. 29, Zhongguancun East Road, Haidian District, Beijing 100190, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
Jianfei
J
Wei
Jun
J
Ren
Jing
J
Liu
Xianwei
X
Meng
Xiangling
X
Ren
Zhenzhen
Z
Chen
Fangqiong
F
Tang
D000109:Acetylcholine / Q000737:chemistry / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D002794:Choline / Q000737:chemistry / Q000302:isolation & purification*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry
D005456:Fluorescent Dyes; D002244:Carbon; D006861:Hydrogen Peroxide; D002794:Choline; D000109:Acetylcholine
Acetylcholine; Carbon dots; Choline; Fenton reaction; Fluorescence