Sampling and decontamination method for culture of nontuberculous mycobacteria in respiratory samples of cystic fibrosis patients.
We confirmed that chlorhexidine decontamination yielded more nontuberculous mycobacteria than did the N-acetyl-l-cysteine-NaOH-oxalic acid procedure from respiratory samples of cystic fibrosis patients on solid cultures. However, this improved recovery is mostly balanced if the latter is combined with liquid culture. Furthermore, none of the 145 cough swabs, used to sample young children, cultured positive, suggesting that swabs are low-quality samples.
Department of Microbiology and Infection Control.
Annelies
A
De Bel
Deborah
D
De Geyter
Iris
I
De Schutter
Christine
C
Mouton
Isabelle
I
Wellemans
Laurence
L
Hanssens
Petra
P
Schelstraete
Anne
A
Malfroot
Denis
D
Pierard
D000293:Adolescent; D000328:Adult; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D002710:Chlorhexidine / Q000494:pharmacology; D003550:Cystic Fibrosis / Q000150:complications*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D009165:Mycobacterium Infections, Nontuberculous / Q000175:diagnosis* / Q000382:microbiology; D009170:Nontuberculous Mycobacteria / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods*; D013183:Sputum / Q000382:microbiology; D055815:Young Adult
D000891:Anti-Infective Agents, Local; D002710:Chlorhexidine