Low magnification confocal microscopy of tumor angiogenesis.
Blood vessels are critical to normal mammalian development, tissue repair, and growth and treatment of cancer. Mouse research models enable mechanistic studies of blood vessels. We detail how to perfuse mice with fluorescent tomato lectin or the lipophilic fluorophore DiI. We provide details on how to image fluorescently labeled blood vessels.
Analytical Imaging Core, Diabetes Research Institute, Miami Institute for Human Genomics, University of Miami, Miami, FL, USA.
George
G
McNamara
Anna
A
Yanai
Vazgen
V
Khankaldyyan
Walter E
WE
Laug
Jeff
J
Boden
Keith
K
Webster
Yiwen
Y
Li
Rong
R
Wen
D000818:Animals; D001808:Blood Vessels / Q000473:pathology / Q000648:ultrastructure*; D004195:Disease Models, Animal; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D006801:Humans; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D009369:Neoplasms / Q000473:pathology*; D009389:Neovascularization, Pathologic / Q000473:pathology*
D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins