Spatiotemporal expression of retrogene-host pair Mcts2/H13 in mouse embryo, and Mcts2 has no influence on H13 transcription pattern in NIH/3T3 cells.
Mcts2 and H13 comprise an imprinted retrogene-host gene pair. Imprinted genes have been proved to be closely related with embryo development. In order to understand its expression relationship during embryo development and influence of the retrogene on the host gene, we studied expression patterns in mouse embryos and transcriptional interference in a cell culture system. The present study determined the spatio-temporal expression pattern of Mcts2 and H13 from embryonic day 9.5 to 15.5. A similar expression pattern between Mcts2 and H13 was observed in mouse embryogenesis by in situ hybridization and real-time PCR, these two genes were extensively expressed in the neural tissues at mid-embryonic stages. As the embryo development proceeded, H13 and Mcts2 were widely detected throughout the developing organism, especially highly expressed in brain. Moreover, neither over expression nor knockdown of Mcts2 has any significant detectable effect on H13 expression in NIH/3T3 cells. In addition, transcriptional up-regulation of Mcts2 caused by demethylation of DMR in the Mcts2 promoter was not directly associated with the H13 transcription in NIH/3T3 cells treated by 5-Aza-cdR. The regulatory relationship between H13 transcripts and the promoter methylation status of Mcts2 was complex, demonstrating host/retrogene relationship may not be limited to the imprinted locus.
School of Life Science and Biotechnology, State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, No. 92 West Da-zhi Street, Harbin, Heilongjiang 150001, China.
Zhijun
Z
Huang
School of Life Science and Biotechnology, State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, No. 92 West Da-zhi Street, Harbin, Heilongjiang 150001, China.
Zhengbin
Z
Han
School of Life Science and Biotechnology, State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, No. 92 West Da-zhi Street, Harbin, Heilongjiang 150001, China.
Fengwei
F
Zhang
School of Life Science and Biotechnology, State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, No. 92 West Da-zhi Street, Harbin, Heilongjiang 150001, China.
Hongjuan
H
He
Department of Respiratory Medicine, the First Affiliated Hospital of Harbin Medical University, No. 199 East Da-zhi Street, Harbin, Heilongjiang 150001, China.
Shihuan
S
Yu
School of Life Science and Biotechnology, State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, No. 92 West Da-zhi Street, Harbin, Heilongjiang 150001, China. Electronic address: Kigo@hit.edu.cn.
Qiong
Q
Wu
D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D015778:Minor Histocompatibility Antigens / Q000235:genetics* / Q000378:metabolism; D041681:NIH 3T3 Cells; D015513:Oncogene Proteins / Q000378:metabolism; D016133:Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D018797:Cell Cycle Proteins; C108856:H13 protein, mouse; C115081:MCTS1 protein, human; D015778:Minor Histocompatibility Antigens; D015513:Oncogene Proteins; D014157:Transcription Factors
H13; Host gene; Mcts2; Mouse embryo; Retrogene; Spatiotemporal expression; Transcriptional interference