[UFLC/Q-TOF-MS based analysis on material base of atractylodis macrocephalae rhizoma stir-fried with wheat bran].
To establish a fingerprint spectrum for Atractylodis Macrocephalae Rhizoma stir-fried with wheat bran based on UFLC/Q-TOF-MS, and make a principal component analysis (PCA) with Markview software, in order to compare the changes of components between raw and processed Atractylodis Macrocephalae Rhizoma with raw wheat bran as the blank. The results showed that the changed in components raw Atractylodis Macrocephalae Rhizoma and Atractylodis Macrocephalae Rhizoma stir-fried with wheat bran were apparently observed by PCA. Six compounds were identified to have significant changes in mass fraction before and after being stir-fried, namely atractylenolide-I, atractylenolide-II, atractylenolide-III, atractylentrid, atractylon and an unknown compound. Among them, atractylenolide-I and atractylenolide-II generated from dehydration and dehydrogenation of atractylenolide-III may be the material base of Atractylodis Macrocephalae Rhizoma stir-fried with wheat bran for strengthening spleen.
Nanjing University of Chinese Medicine, Nanjing 210023, China. xiaobingcui@163.com
Xiao-Bing
XB
Cui
Chen-Xiao
CX
Shan
Hong-Mei
HM
Wen
Wei
W
Li
Hao
H
Wu
D031174:Atractylodes / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D004043:Dietary Fiber; D007783:Lactones / Q000032:analysis; D013058:Mass Spectrometry; D025341:Principal Component Analysis; D012717:Sesquiterpenes / Q000032:analysis
D004043:Dietary Fiber; D007783:Lactones; D012717:Sesquiterpenes; C424804:atractylenolide I; C458582:atractylenolide II; C424802:atractylenolide III