[Dynamic changes of enzyme activities and active component contents in Lonicera japonica during different blossoming stages].
OBJECTIVE
This paper aimed to study the dynamic changes of enzyme activities and active component contents in Lonicera japonica during different blossoming stages.


METHODS
The enzyme activities of phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), peroxidase (POD) and the contents of total phenol, total flavonoids, chlorogenic acid, anthocyanins in L. japonica during different blossoming stages were determined.


RESULTS
The contents of total phenolics, total flavonoids, anthocyanins decreased from the Sanqing stage to Jinhua stage while the content of chlorogenic acid increased slightly in white period, and then decreased gradually. The activities of three enzymes decreased gradually from Sanqing stage, and got to a minimum value in Yinhua stage, then increased slightly until the Jinhua stage.


CONCLUSIONS
The enzyme activities of PPO and POD correlated the content of phenolic substances positively before the Jinhua stage in L. japonica. In the period of maturity, the POD activity was strengthened due to the induction of respiration and became the key enzyme to control active component content during the mature stage.
Collage of Tea and Food Science and Technology, Anhui Agricultural University, Hefei 230036, China. lnn0413@163.com
Na-Na
NN
Liu
Wei
W
Liu
Jing
J
Feng
Yi-Bin
YB
Zhou
Jian-Hua
JH
Liu
Xiao
X
Wang
Sheng-Bo
SB
Li
Yong-Qing
YQ
Zhang
D004156:Catechol Oxidase / Q000378:metabolism; D035264:Flowers; D031239:Lonicera / Q000737:chemistry* / Q000201:enzymology; D009195:Peroxidase / Q000378:metabolism; D010636:Phenols / Q000032:analysis
D010636:Phenols; D004156:Catechol Oxidase; D009195:Peroxidase