Direct production of feruloyl oligosaccharides and hemicellulase inducement and distribution in a newly isolated Aureobasidium pullulans strain.
Studies were carried out to screen and identify strains that are able to directly produce ferulic oligosaccharides (FOs) from wheat bran (WB). The inducement and distribution of hemicellulases from strain 2012, which was identified as a non-melanin secreting strain of Aureobasidium pullulans (A. pullulans), were also determined. In a 60 g/L WB solution, A. pullulans could produce 545 nmol/L FOs, 64.12 IU/mL xylanase and 0.14 IU/mL ferulic acid esterase (FAE). A. pullulans was cultivated in media with WB, glucose, xylose, sucrose, lactose or xylan as the carbon source, and hemicellulases were mainly induced by xylan and WB and inhibited by glucose and sucrose. Xylanase and FAE were mainly present in the culture filtrate, xylosidase in the hyphal filaments and arabinofuranosidase was a membrane-bound enzyme. The yield of FOs was positively correlated to the hemicellulases activity, and significantly positively (P < 0.05) correlated to the xylanase activity (r = 0.992).
Xiao-hong
XH
Yu
Zhen-xin
ZX
Gu
D001203:Ascomycota / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004043:Dietary Fiber / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D014995:Xylosidases / Q000378:metabolism
D003470:Culture Media; D004043:Dietary Fiber; D009844:Oligosaccharides; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; D006026:Glycoside Hydrolases; D014995:Xylosidases; C023305:hemicellulase