Sphingobacterium arenae sp. nov., isolated from sandy soil.
A novel, Gram-stain-negative, non-motile, non-spore-forming, short rod-shaped, aerobic bacterium designated H-12(T) was isolated from a mixed sandy soil sample collected from Xinjiang, China. Strain H-12(T) grew at 20-37 °C (optimum, 30 °C), pH 7.0-11.0 (optimum, pH 8.5) on TGY medium with 0-5 % NaCl (w/v). 16S rRNA gene sequence analysis indicated that strain H-12(T) shared sequence similarities with Sphingobacterium composti DSM 18850(T) (90.0 %). Strain H-12(T) showed a low level of DNA-DNA relatedness to Sphingobacterium composti DSM 18850(T) (45.5 %). The predominant isoprenoid quinone of strain H-12(T) was MK-7 and the major polar lipid was phosphatidylethanolamine. The predominant cellular fatty acids were C16:1ω7c and/or C16 : 1ω6c, iso-C15 : 0, iso-C17 : 0 3-OH, C16 : 0, C16 : 0 3-OH and iso-C15 : 0 3-OH. DNA G+C content of strain H-12(T) was 44.15 mol%. On the basis of phenotypic, genetic and phylogenetic data, strain H-12(T) is proposed to be a representative of a novel species of the genus Sphingobacterium, for which the name Sphingobacterium arenae sp. nov. is suggested and the type strain is H-12(T) ( = ACCC 05758(T) = KCTC 32294(T)).
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.|Life Science and Engineering College, Southwest University of Science and Technology, Mianyang, Sichuan 621010, PR China.
Shijie
S
Jiang
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Ming
M
Chen
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Shiyou
S
Su
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Mingkun
M
Yang
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
Aihua
A
Li
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Chen
C
Zhang
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Min
M
Lin
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Wei
W
Zhang
Life Science and Engineering College, Southwest University of Science and Technology, Mianyang, Sichuan 621010, PR China.
Xuegang
X
Luo
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012822:Silicon Dioxide; D012988:Soil Microbiology; D045249:Sphingobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; D012822:Silicon Dioxide; C062629:menaquinone 7