Characterization of the pumpkin Translationally-Controlled Tumor Protein CmTCTP.
In higher plants, the phloem plays a central role in the delivery of nutrients and signals from source to sink tissues. These signals likely coordinate different aspects of plant development, as well as its response to environmental cues. Although some phloem-transported proteins and RNAs may function as signaling molecules in plants, their mode of action remains poorly understood. Previous analysis of transcripts from CMV-infected pumpkin (Cucurbita maxima cv Big Max) identified a Translationally-Controlled Tumor Protein (TCTP) mRNA homolog, designated CmTCTP. In the present work this transcript was analyzed in terms of its expression pattern. This RNA accumulates, both in healthy and CMV-infected plants, in developing and mature phloem in petiole and roots, as well as in apices at high levels. The protein was present at lower levels in most cell types, and almost no signal was detected in apices, suggesting translational regulation of this RNA. Additionally, CmTCTP harbored by Agrobacterium rhizogenes is capable of inducing whole plant regeneration. These data suggest a role for CmTCTP in growth regulation, possibly through long-distance signaling.
Departamento de Biotecnología y Bioingeniería; CINVESTAV- IPN; Zacatenco, D.F. Mexico; Facultad de Ingeniería Química; Benemérita Universidad Autónoma de Puebla; Colonia San Manuel; Ciudad Universitaria; Puebla, México.
J Jesús
JJ
Hinojosa-Moya
Departamento de Biotecnología y Bioingeniería; CINVESTAV- IPN; Zacatenco, D.F. Mexico.
Beatriz
B
Xoconostle-Cázares
Departamento de Biotecnología y Bioingeniería; CINVESTAV- IPN; Zacatenco, D.F. Mexico.
Roberto
R
Toscano-Morales
Departamento de Biotecnología y Bioingeniería; CINVESTAV- IPN; Zacatenco, D.F. Mexico.
Francisco
F
Ramírez-Ortega
Departamento de Ingeniería Genética de Plantas; CINVESTAV-IPN Unidad Guanajuato; Irapuato, Guanajuato México.
José Luis
JL
Cabrera-Ponce
Departamento de Biotecnología y Bioingeniería; CINVESTAV- IPN; Zacatenco, D.F. Mexico.
Roberto
R
Ruiz-Medrano
D060054:Agrobacterium / Q000502:physiology; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D028464:Cucurbita / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D052585:Phloem / Q000378:metabolism; D018515:Plant Leaves / Q000382:microbiology / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012038:Regeneration; D014026:Nicotiana / Q000382:microbiology / Q000502:physiology; D000090324:Tumor Protein, Translationally-Controlled 1
D014408:Biomarkers, Tumor; D010940:Plant Proteins; D012333:RNA, Messenger; D000090324:Tumor Protein, Translationally-Controlled 1
Agrobacterium rhizogenes; Phloem; Translationally Controlled Tumor Protein; long-distance signaling; plant regeneration