Leucine-rich α2-glycoprotein is a novel biomarker of neurodegenerative disease in human cerebrospinal fluid and causes neurodegeneration in mouse cerebral cortex.
Leucine-rich α2-glycoprotein (LRG) is a protein induced by inflammation. It contains a leucine-rich repeat (LRR) structure and easily binds with other molecules. However, the function of LRG in the brain during aging and neurodegenerative diseases has not been investigated. Here, we measured human LRG (hLRG) concentration in the cerebrospinal fluid (CSF) and observed hLRG expression in post-mortem human cerebral cortex. We then generated transgenic (Tg) mice that over-expressed mouse LRG (mLRG) in the brain to examine the effects of mLRG accumulation. Finally, we examined protein-protein interactions using a protein microarray method to screen proteins with a high affinity for hLRG. The CSF concentration of hLRG increases with age and is significantly higher in patients with Parkinson's disease with dementia (PDD) and progressive supranuclear palsy (PSP) than in healthy elderly people, idiopathic normal pressure hydrocephalus (iNPH) patients, and individuals with Alzheimer's disease (AD). Tg mice exhibited neuronal degeneration and neuronal decline. Accumulation of LRG in the brains of PDD and PSP patients is not a primary etiological factor, but it is thought to be one of the causes of neurodegeneration. It is anticipated that hLRG CSF levels will be a useful biomarker for the early diagnosis of PDD and PSP.
Department of Neurosurgery, Juntendo University Graduate School of Medicine, Tokyo, Japan.
Masakazu
M
Miyajima
Madoka
M
Nakajima
Yumiko
Y
Motoi
Masao
M
Moriya
Hidenori
H
Sugano
Ikuko
I
Ogino
Eri
E
Nakamura
Norihiro
N
Tada
Miyuki
M
Kunichika
Hajime
H
Arai
D000368:Aged; D000375:Aging / Q000134:cerebrospinal fluid / Q000473:pathology; D000818:Animals; D019540:Area Under Curve; D001344:Autopsy; D015415:Biomarkers / Q000134:cerebrospinal fluid; D001921:Brain / Q000473:pathology; D002460:Cell Line; D002540:Cerebral Cortex / Q000378:metabolism* / Q000473:pathology*; D003710:Demography; D005260:Female; D006023:Glycoproteins / Q000134:cerebrospinal fluid* / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009410:Nerve Degeneration / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D019636:Neurodegenerative Diseases / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D011485:Protein Binding
D015415:Biomarkers; D006023:Glycoproteins; C015246:LRG1 protein, human; C530631:LRG1 protein, mouse