Molecular and serological prevalence of Babesia bigemina and Babesia bovis in cattle and water buffalos under small-scale dairy farming in Beheira and Faiyum Provinces, Egypt.
In order to determine the molecular and serological prevalence of Babesia bigemina and Babesia bovis, a total of 247 blood samples were collected from cattle and water buffalos in Beheira and Faiyum Provinces in Egypt and examined by nested polymerase chain reaction (nPCR) and enzyme-linked immunosorbent assay (ELISA). In cattle, the prevalence of B. bigemina and B. bovis was 5.30% and 3.97% by nPCR and 10.60% and 9.27% by ELISA, respectively, whereas those of water buffalos were 10.42% and 4.17% by nPCR and 15.63% and 11.46% by ELISA, respectively. Statistically significant differences in the prevalence of the two infections were observed on the basis of age and health status. Sequencing analysis revealed two genotypes for B. bovis spherical body protein-4. In conclusion, the current data provide valuable information regarding the epidemiology of B. bigemina and B. bovis infections in cattle and water buffalos from Egypt, which can be employed in developing future strategies for disease management and control.
Department of Zoology, Faculty of Science, Minufiya University, Shebin El-Kom, Egypt.
Hany M
HM
Ibrahim
Paul F
PF
Adjou Moumouni
Khaled
K
Mohammed-Geba
Sherin K
SK
Sheir
Ihab S Y
IS
Hashem
Shinuo
S
Cao
Mohamad A
MA
Terkawi
Ketsarin
K
Kamyingkird
Yoshifumi
Y
Nishikawa
Hiroshi
H
Suzuki
Xuenan
X
Xuan
D000375:Aging; D000818:Animals; D001403:Babesia / Q000145:classification* / Q000235:genetics; D001404:Babesiosis / Q000097:blood / Q000453:epidemiology / Q000662:veterinary*; D002020:Buffaloes; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology*; D016054:DNA, Protozoan / Q000097:blood; D003612:Dairying; D004534:Egypt / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005838:Genotype; D016133:Polymerase Chain Reaction; D015995:Prevalence; D016036:Seroepidemiologic Studies
D016054:DNA, Protozoan
Babesia bigemina; Babesia bovis; ELISA; Egypt; Nested PCR