Using Arabidopsis-related model species (ARMS): growth, genetic transformation, and comparative genomics.
The Arabidopsis-related model species (ARMS) Thellungiella salsuginea and Thellungiella parvula have generated broad interest in salt stress research. While general growth characteristics of these species are similar to Arabidopsis, some aspects of their life cycle require particular attention in order to obtain healthy plants, with a large production of seeds in a relatively short time. This chapter describes basic procedures for growth, maintenance, and Agrobacterium-mediated transformation of ARMS. Where appropriate, differences in requirements between Thellungiella spp. and Arabidopsis are highlighted, along with basic growth requirements of other less studied candidate model species. Current techniques for comparative genomics analysis between Arabidopsis and ARMS are also described in detail.
CNR-IGV Institute of Plant Genetics, Portici, Italy.
Giorgia
G
Batelli
Dong-Ha
DH
Oh
Matilde Paino
MP
D'Urzo
Francesco
F
Orsini
Maheshi
M
Dassanayake
Jian-Kang
JK
Zhu
Hans J
HJ
Bohnert
Ray A
RA
Bressan
Albino
A
Maggio
D060054:Agrobacterium / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D019607:Brassicaceae / Q000235:genetics / Q000254:growth & development; D046508:Culture Techniques; D018745:Genome, Plant; D018525:Germination; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development; D011309:Preservation, Biological; D055051:Salt-Tolerant Plants / Q000235:genetics / Q000254:growth & development; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D014170:Transformation, Genetic