Improvement of a rapid screening test for chronic granulomatous disease.
Diagnosis of CGD is made by demonstrating absent or markedly reduced oxidase activity in stimulated neutrophils. The screening test proposed is based upon the naked eye evaluation of the reduction of NBT on a solid surface. It seems to be a useful tool for rapid and inexpensive detection of CGD patients, especially for large-scale screening purposes. The test was carried out on forty-five subjects: two males affected by CGD, three female carriers and forty healthy donors. The test confirmed the results obtained with flow cytometric and NBT assays.
Department of Pediatrics, Policlinico Umberto I, Sapienza University of Rome, Rome, Italy.
M
M
Iacobini
M
M
Duse
A
A
Di Coste
L
L
Balducci
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D004396:Coloring Agents; D005260:Female; D005434:Flow Cytometry; D006105:Granulomatous Disease, Chronic / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D008297:Male; D009580:Nitroblue Tetrazolium; D010586:Phagocytes / Q000737:chemistry*; D011237:Predictive Value of Tests; D013997:Time Factors; D055815:Young Adult
D004396:Coloring Agents; D009580:Nitroblue Tetrazolium