PBP deletion mutants of Escherichia coli exhibit irregular distribution of MreB at the deformed zones.
MreB is a cytoskeletal protein, which is responsible for maintaining proper cellular morphology and is essential for cell survival. Likewise, penicillin-binding protein 5 (PBP5) helps in maintaining cell shape, though non-essential for survival. The contradicting feature of these two proteins paves the way for this study, wherein we attempt to draw a relation on the nature of distribution of MreB in PBP deletion mutants. The study revealed that the uniform MreB helices/patches were destabilized/disturbed at the zone of deformities of the PBP mutants, whereas the helical patterns were retained at the regions maintaining a rod shape. We interpret that MreB remains functional irrespective of its distribution being misguided by the aberrant shapes of PBP mutants.
Department of Biotechnology, Indian Institute of Technology Kharagpur (IIT Kgp), Kharagpur, 721302, India, sapthavijayan.iitkgp@gmail.com.
Saptha
S
Vijayan
Sathi
S
Mallick
Mouparna
M
Dutta
M
M
Narayani
Anindya S
AS
Ghosh
D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D046915:Penicillin-Binding Proteins / Q000235:genetics*; D021381:Protein Transport; D017384:Sequence Deletion
D029968:Escherichia coli Proteins; D046915:Penicillin-Binding Proteins; C078473:MreB protein, E coli