Cloning, distribution and effects of fasting status of melanocortin 4 receptor (MC4R) in Schizothorax prenanti.
Melanocortin 4 receptor (MC4R) has an important role in the regulation of energy homeostasis in both mammals and fish. In this study, MC4R was characterized in S. prenanti (Schizothorax prenanti) and designated as SpMC4R. SpMC4R cDNA is composed of 1004 nucleotides with a 978 nucleotide open reading frame encoding a protein of 326 amino acids. The SpMC4R contained predicted regions that were structural features of MCR subtypes of vertebrates. In addition, phylogenetic analyses suggested that S. prenanti MC4R was closely related to fish MC4Rs. The SpMC4R mRNA was detected in embryos at developmental stages. Further, its mRNA was detectable in unfertilized eggs. Using real-time RT-PCR, MC4R is widely expressed, with highest levels of expression in brain and ovary. An experiment was conducted to determine the expression profile of MC4R during short-term and long-term fasting of the brain. The expression level of MC4R in unfed fish was significantly increased at 6, 9 and 24h post-fasting (hpf) and 14days fasting than in fed fish, this suggests that MC4R is conserved peptide that might be involved in the regulation of food intake and other physiological function in S. prenanti.
Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Yaan, China.
Rongbin
R
Wei
Dengyue
D
Yuan
Chaowei
C
Zhou
Tao
T
Wang
Fangjun
F
Lin
Hu
H
Chen
Hongwei
H
Wu
Zhiming
Z
Xin
Shiyong
S
Yang
Defang
D
Chen
Yan
Y
Wang
Ju
J
Liu
Yundi
Y
Gao
Zhiqiong
Z
Li
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000502:physiology; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000196:embryology / Q000235:genetics / Q000502:physiology*; D004625:Embryo, Nonmammalian; D005215:Fasting; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008969:Molecular Sequence Data; D010053:Ovary / Q000502:physiology; D010802:Phylogeny; D044105:Receptor, Melanocortin, Type 4 / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D044105:Receptor, Melanocortin, Type 4
AgRP; Appetite regulation; Cloning; ECL; FTO; G protein-coupled receptors; GPCRs; Gene evolution; ICL; MC; MCRs; MS-222; NPY; ORF; POMC; Schizothorax prenanti; Structure; TM; agouti-related protein; base pair; bp; extracellular loops; hour post spawning; hps; intracellular loops; melanocortin; melanocortin receptors; neuropeptide Y; obesity-associated; open reading frame; proopiomelanocortin; transmembrane domains; tricaine methanesulfonate; α-MSH; α-melanocyte-stimulating hormone