A partial deletion in non-structural protein 3A can attenuate foot-and-mouth disease virus in cattle.
The role of non-structural protein 3A of foot-and-mouth disease virus (FMDV) on the virulence in cattle has received significant attention. Particularly, a characteristic 10-20 amino acid deletion has been implicated as responsible for virus attenuation in cattle: a 10 amino acid deletion in the naturally occurring, porcinophilic FMDV O1 Taiwanese strain, and an approximately 20 amino acid deletion found in egg-adapted derivatives of FMDV serotypes O1 and C3. Previous reports using chimeric viruses linked the presence of these deletions to an attenuated phenotype in cattle although results were not conclusive. We report here the construction of a FMDV O1Campos variant differing exclusively from the highly virulent parental virus in a 20 amino acid deletion between 3A residues 87-106, and its characterization in vitro and in vivo. We describe a direct link between a deletion in the FMDV 3A protein and disease attenuation in cattle.
Agricultural Research Service, U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY, USA.
Juan M
JM
Pacheco
Douglas P
DP
Gladue
Lauren G
LG
Holinka
Jonathan
J
Arzt
Elizabeth
E
Bishop
George
G
Smoliga
Steve J
SJ
Pauszek
Alexa J
AJ
Bracht
Vivian
V
O'Donnell
Ignacio
I
Fernandez-Sainz
Paige
P
Fletcher
Maria E
ME
Piccone
Luis L
LL
Rodriguez
Manuel V
MV
Borca
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000473:pathology* / Q000821:virology; D005536:Foot-and-Mouth Disease / Q000473:pathology* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000472:pathogenicity*; D008969:Molecular Sequence Data; D009297:Nasal Mucosa / Q000821:virology; D016415:Sequence Alignment; D017384:Sequence Deletion; D044967:Serum / Q000821:virology; D019562:Viral Load; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*; D010948:Viral Plaque Assay; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
D017361:Viral Nonstructural Proteins; D037521:Virulence Factors