Protective effect of gallic acid on alloxan-induced oxidative stress and osmotic fragility in rats.
In the present study, we investigated the antioxidant effect of gallic acid (GA) on membrane lipid peroxidation and osmotic fragility in alloxan-induced diabetic Wistar rats. GA was administered orally at doses of 5, 10, and 20 mg/kg body weight for 45 days, after which liver and kidney tissues were analyzed for the degree of lipid peroxidation, reduced glutathione, and the activities of antioxidants such as catalase, superoxide dismutase, and glutathione peroxidase. Administration of GA to alloxan-induced diabetic rats reduced the blood glucose level with an increase in the level of insulin. Liver and kidney tissues from diabetic animals exhibited disturbances in antioxidant defense compared with normal rats. GA at a dose of 20 mg/kg b.w. showed a significant effect than that of the other doses. In addition, the results revealed that GA protected the integrity of erythrocyte membrane in diabetic rats as demonstrated by lower percentage of hemolysis and resistance to hydrogen peroxide-induced peroxidation. The anti-hyperglycemic activity of GA in alloxan-induced diabetic rats was also comparable with glibenclamide, a reference drug. These results suggest that GA could provide a beneficial effect on diabetes by decreasing oxidative stress-related diabetic complications.
SRM Research Institute, SRM University, Kattankulathur, Chennai, Tamil Nadu, India ramkumar.km@res.srmuniv.ac.in.
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Ramkumar
Institute of Biotechnology and Pharmaceutical Research, The National Health Research Institutes, Zhunan, Miaoli County, Taiwan.
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Vijayakumar
SRM Research Institute, SRM University, Kattankulathur, Chennai, Tamil Nadu, India.
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Vanitha
SRM Research Institute, SRM University, Kattankulathur, Chennai, Tamil Nadu, India.
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Suganya
Department of Biotechnology, Anna University-BIT Campus, Tiruchirappalli, Tamil Nadu, India.
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C
Manjula
Department of Biotechnology, Anna University-BIT Campus, Tiruchirappalli, Tamil Nadu, India.
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Rajaguru
King Institute of Preventive Medicine and Research, Guindy, Chennai, Tamil Nadu, India.
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Sivasubramanian
King Institute of Preventive Medicine and Research, Guindy, Chennai, Tamil Nadu, India.
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Gunasekaran
D000496:Alloxan; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D019610:Cytoprotection; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D005707:Gallic Acid / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005905:Glyburide / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D009996:Osmotic Fragility / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
D000975:Antioxidants; D015415:Biomarkers; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D005707:Gallic Acid; D000496:Alloxan; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D005905:Glyburide
Diabetes; RBCs; alloxan; antioxidants; gallic acid; oxidative stress