Quantitative evaluation of the mitochondrial proteomes of Drosophila melanogaster adapted to extreme oxygen conditions.
Mitochondria are the primary organelles that consume oxygen and provide energy for cellular activities. To investigate the mitochondrial mechanisms underlying adaptation to extreme oxygen conditions, we generated Drosophila strains that could survive in low- or high-oxygen environments (LOF or HOF, respectively), examined their mitochondria at the ultrastructural level via transmission electron microscopy, studied the activity of their respiratory chain complexes, and quantitatively analyzed the protein abundance responses of the mitochondrial proteomes using Isobaric tag for relative and absolute quantitation (iTRAQ). A total of 718 proteins were identified with high confidence, and 55 and 75 mitochondrial proteins displayed significant differences in abundance in LOF and HOF, respectively, compared with the control flies. Importantly, these differentially expressed mitochondrial proteins are primarily involved in respiration, calcium regulation, the oxidative response, and mitochondrial protein translation. A correlation analysis of the changes in the levels of the mRNAs corresponding to differentially regulated mitochondrial proteins revealed two sets of proteins with different modes of regulation (transcriptional vs. post-transcriptional) in both LOF and HOF. We believe that these findings will not only enhance our understanding of the mechanisms underlying adaptation to extreme oxygen conditions in Drosophila but also provide a clue in studying human disease induced by altered oxygen tension in tissues and cells.
Chinese Academy of Sciences Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China ; University of Chinese Academy of Sciences, Beijing, China.
Songyue
S
Yin
Jin
J
Xue
Haidan
H
Sun
Bo
B
Wen
Quanhui
Q
Wang
Guy
G
Perkins
Huiwen W
HW
Zhao
Mark H
MH
Ellisman
Yu-hsin
YH
Hsiao
Liang
L
Yin
Yingying
Y
Xie
Guixue
G
Hou
Jin
J
Zi
Liang
L
Lin
Gabriel G
GG
Haddad
Dan
D
Zhou
Siqi
S
Liu
D000222:Adaptation, Physiological; D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D004579:Electron Transport; D018496:Hyperoxia / Q000235:genetics / Q000378:metabolism; D000860:Hypoxia / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010641:Phenotype; D020543:Proteome; D040901:Proteomics / Q000379:methods; D059467:Transcriptome
D024101:Mitochondrial Proteins; D020543:Proteome