[Investigation on bioactive protection of LEA protein for insulin by molecular simulation in the low-temperature drying process].
Nowadays various protein medicines are increasingly playing significant roles in the treatment of many diseases, but the bioactive structures of such kinds of protein medicines are unstable because they are heat sensitive. Therefore, it is very important to explore a protective method and to explain the protective mechanism of protein medicines. In the present research, insulin was chosen as a heat-sensitive protein medicine, and a Group 3 late embryogenesis abundant (LEA) protein was chosen as its bioactive protectant during desiccation. The results of replica exchange molecular dynamics simulation suggest that comparing with insulin without any protection, the bioactive 3D structure and secondary structure of the insulin protected by LEA protein were preserved very well. All analyzing results proved that the LEA protein was a good bioactive protectant for heat sensitive protein medicines.
Institute of Biothermal Science and Technology, University of Shanghai for Science and Technology, Shanghai 200093, China. dxli75@126.com
Daixi
D
Li
Yan
Y
Zhang
Baisong
B
Guo
Baolin
B
Liu
Chunsheng
C
Yang
Yaru
Y
Liu
Zhen
Z
Zhai
D000818:Animals; D003080:Cold Temperature; D004355:Drug Stability; D005612:Freeze Drying; D015801:Helminth Proteins / Q000737:chemistry*; D007328:Insulin / Q000737:chemistry*; D009348:Nematoda; D010940:Plant Proteins / Q000494:pharmacology; D054730:Protein Interaction Domains and Motifs; D017433:Protein Structure, Secondary
D015801:Helminth Proteins; D007328:Insulin; D010940:Plant Proteins; C066246:late embryogenesis abundant protein, plant