Toxicity assessments with Daphnia magna of Guadipyr, a new neonicotinoid insecticide and studies of its effect on acetylcholinesterase (AChE), glutathione S-transferase (GST), catalase (CAT) and chitobiase activities.
Guadipyr is a novel neonicotinoid insecticide, developed by the China Agricultural University. This work investigated its aquatic toxicity on Daphnia magna. The acute immobilization test showed that guadipyr was slightly toxic to daphnids, with a 48 h EC₅₀ of 13.01 mg/L. In addition, guadipyr significantly enhanced the acetylcholinesterase (AChE) and glutathione S-transferase (GST) activity (per gram of protein), but had no obvious impact on catalase (CAT) activity within 48 h. The 21 d chronic exposure of D. magna to guadipyr induced a significant decrease in body growth and reproduction; both share the same lowest observed effect concentration (LOEC) at 0.10 mg/L. In the 14 d chronic test, a significant increase in chitobiase activity in test media was observed at day 8 (days to the first breeding), while a significant decrease was observed from days 10 to 14, which might be due to the endocrine imbalance resulting from guadipyr stress. These results demonstrated that guadipyr can induce notable negative ecotoxicological impacts on the aquatic system in long-term exposure at a sub-lethal dose. Further research in environmental behaviors is needed to regulate guadipyr use in the future.
Key Laboratory of Pesticide Chemistry & Application Technology, Ministry of Agriculture, College of Science, China Agricultural University, Beijing 100193, PR China.
Suzhen
S
Qi
Chen
C
Wang
Xiaofeng
X
Chen
Zhaohai
Z
Qin
Xuefeng
X
Li
Chengju
C
Wang
D000110:Acetylcholinesterase / Q000378:metabolism*; D000118:Acetylglucosaminidase / Q000378:metabolism*; D000818:Animals; D002374:Catalase / Q000378:metabolism*; D003621:Daphnia / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D005982:Glutathione Transferase / Q000378:metabolism*; D006146:Guanidines / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D012098:Reproduction / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000633:toxicity*
C587227:Guadipyr; D006146:Guanidines; D007306:Insecticides; D014874:Water Pollutants, Chemical; D002374:Catalase; D005982:Glutathione Transferase; D000110:Acetylcholinesterase; D000118:Acetylglucosaminidase
Acute toxicity; Chitobiase activity; Chronic toxicity; Guadipyr; Zooplankton