The peel and pulp of mango fruit: a proteomic samba.
Combinatorial peptide ligand libraries (CPLLs) have been adopted for investigating the proteomes of mango peel and pulp as well their peptidome content (the latter as captured with a C18 resin). The aim of this study was not only to perform the deepest investigation so far of the mango proteome, but also to assess the potential presence of allergens and of peptides endowed with biological activities. The proteins of peel and pulp have been captured under both native and denaturing extraction techniques. A total of 334 unique protein species have been identified in the peel vs. 2855 in the pulp, via capture with CPLLs at different pH values (2.2 and 7.2).
Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, 20131 Milan, Italy.
Elisa
E
Fasoli
Pier Giorgio
PG
Righetti
D000485:Allergens / Q000737:chemistry / Q000378:metabolism*; D005638:Fruit / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D031022:Mangifera / Q000737:chemistry / Q000378:metabolism*; D019151:Peptide Library; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods
D000485:Allergens; D019151:Peptide Library; D010940:Plant Proteins; D020543:Proteome
CPLLs; Combinatorial peptide ligand libraries; Low abundance proteome; Mango peel; Mango pulp; Mass spectrometry; PM; ProteoMiner; combinatorial peptide ligand libraries