[Analysis of endophytic fungi community of Ligusticum chuanxiong using PCR-DGGE].
OBJECTIVE
To research the diversity of endophytic fungal communities among Ligusticum chuanxiong growing at 5 areas in Sichuan province, and illuminate the developing mechanism of geoherbs from the microecological perspective.


METHODS
The PCR-DGGE and DNA sequencing techniques were used to analyze the endophytic fungi community of L. chuanxiong.


RESULTS
The community of endophytic fungi present difference among different growing areas. Though minor difference were found among individuals at the same area, similarity among individuals from the same growing areas were higher significantly than those from different growing areas. Compared with the other 4 growing areas, L. chuanxiong from Shiyang town, Dujiangyan city had more abundant endophytic fungi and low similarity to others, and which probably had special types of fungi.


CONCLUSIONS
The abundant and stable endophytic fungal community is an important factor for the development of geoherb L. chuanxiong at Shiyang town, Dujiangyan city.
Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine State, Key Labratorry Breeding Base of systematic Research Development and Utilization of Chinese Medicine Resourses, Chengdu 611137, China. wangpenghai@163.com
Hai
H
Wang
Zhu-Yun
ZY
Yan
Dong-Mei
DM
He
Meng
M
Wang
D058645:Denaturing Gradient Gel Electrophoresis / Q000379:methods*; D030004:Ligusticum / Q000235:genetics* / Q000254:growth & development; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA