miR-370 targeted FoxM1 functions as a tumor suppressor in laryngeal squamous cell carcinoma (LSCC).
microRNAs, a family of small non-coding RNAs, involve in the pathogenesis of several types of cancers, including laryngeal squamous cell carcinoma (LSCC). MiR-370 is frequently aberrant expressed in various types of human cancer including LSCC. However, the role for miR-370 in LSCC remains elusive. Here, we demonstrate that miR-370 was down-regulated in human LSCC tissues. Furthermore, there was an inverse relationship between Forkhead Box ml (FoxM1), which was up-regulated and miR-370 expression in LSCC tissues. FoxM1 was subsequently predicted by bioinformatics and verified to be a target of miR-370 by Luciferase reporter assay. Restored expression of miR-370 in Hep2 cells significantly inhibited cell proliferation. In conclusion, our results suggest that miR-370 may function as a tumor suppressor in LSCC through downregulation of FoxM1, suggesting that miR-370 could serve as a novel potential maker for LSCC therapy.
Department of Otolaryngology-Head and Neck Surgery, Qi-Lu Hospital, Shandong University, Jinan, Shandong Province, PR China; Department of Otolaryngology-Head and Neck Surgery, the Affiliated Hospital of Jining Medical University, Jining, Shandong Province, PR China.
Wu
W
Yungang
Department of Otolaryngology-Head and Neck Surgery, the Affiliated Hospital of Jining Medical University, Jining, Shandong Province, PR China.
Li
L
Xiaoyu
Department of Otolaryngology-Head and Neck Surgery, the Affiliated Hospital of Jining Medical University, Jining, Shandong Province, PR China.
Taizhong
T
Pang
Department of Otolaryngology-Head and Neck Surgery, Qi-Lu Hospital, Shandong University, Jinan, Shandong Province, PR China.
Li
L
Wenming
Department of Otolaryngology-Head and Neck Surgery, Qi-Lu Hospital, Shandong University, Jinan, Shandong Province, PR China. Electronic address: panxinliang0531@sina.com.
Xinliang
X
Pan
D001483:Base Sequence; D001665:Binding Sites; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors / Q000235:genetics*; D017930:Genes, Reporter; D016147:Genes, Tumor Suppressor; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D049410:Luciferases, Renilla / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D014162:Transfection; D015854:Up-Regulation
C107585:FOXM1 protein, human; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors; C526503:MIRN370 microRNA, human; D035683:MicroRNAs; D049410:Luciferases, Renilla
Cell proliferation; FoxM1; Laryngeal squamous cell carcinoma; MiR-370