Twitching motility activity, biofilm formation, and genetic typing for clinical isolates of Pseudomonas aeruginosa by random amplified DNA PCR.
A total of 44 Pseudomonas aeruginosa clinical strains were studied by random amplified polymorphic DNA PCR. (RAPD)-PCR analysis determined the presence of 15 genotypes, with the most frequent genotype A detected in 27.3% of the strains. It was observed that clonally related strains were isolated from patients within the same ward and among different wards of two hospitals. The collection of P. aeruginosa was also screened in microtiter plates made of polystyrene to test for their ability to form a biofilm on an abiotic surface. Generally most of the strains (88.6%) showed a significant ability to form biofilm. We found a correlation between twitching motility activity and between biofilm production and source of isolation of strains. No correlation was observed between P. aeruginosa strain genotypes and biofilm formation, as well as source and place of isolation.
University of Natural Sciences and Humanities in Siedlce Department of Microbiology, Nature Faculty Siedlce Poland.
Katarzyna
K
Wolska
Barbara
B
Kot
D015373:Bacterial Typing Techniques; D018441:Biofilms / Q000254:growth & development; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D006801:Humans; D010641:Phenotype; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*
D004269:DNA, Bacterial; D005819:Genetic Markers