Efficient growth inhibition of EGFR over-expressing tumor cells by an anti-EGFR nanobody.
Epidermal growth factor receptor (EGFR) is deemed to be one of the main molecular targets for diagnosis and treatment of cancer. It has been identified that EGFR involves in pathogenesis of some forms of human cancers. Monoclonal antibodies targeting EGFR could control the tumor cell growth, proliferation, and apoptosis by suppressing the signal transduction pathways. Nanobodies can be regarded as the smallest intact antigen binding fragments, derived from heavy chain-only antibodies existing in camelids. Here, we describe the identification of an EGFR-specific nanobody, referred to as OA-cb6, obtained from immunized camel with a cell line expressing high levels of EGFR. Utilizing flow cytometry (FACS) and blotting methods, we demonstrated that OA-cb6 nanobody binds specifically to EGFR expressing on the surface of A431 cells. In addition, OA-cb6 nanobody potently causes the inhibition of EGFR over expression, cell growth and proliferation. The antibody fragments can probably be regarded as worthwhile binding block for further rational design of anti-cancer therapy.
Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, P.O. Box 14395/1179, Tehran, Iran, omidfar@tums.ac.ir.
Kobra
K
Omidfar
Fatemeh Sadat
FS
Amjad Zanjani
Arghavan Golbaz
AG
Hagh
Maedeh Darziani
MD
Azizi
Seyed Javad
SJ
Rasouli
Susan
S
Kashanian
D000818:Animals; D000915:Antibody Affinity / Q000276:immunology; D000918:Antibody Specificity; D002162:Camelus; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D066246:ErbB Receptors / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D056724:Immunity, Humoral / Q000187:drug effects; D009369:Neoplasms / Q000473:pathology*; D061905:Single-Domain Antibodies / Q000302:isolation & purification / Q000494:pharmacology*
D061905:Single-Domain Antibodies; C512478:EGFR protein, human; D066246:ErbB Receptors