Pontibacter indicus sp. nov., isolated from hexachlorocyclohexane-contaminated soil.
An orange-pigmented bacterial strain, designated LP100(T), was isolated from hexachlorocyclohexane-contaminated soil (Lucknow, India). A neighbour-joining tree based on 16S rRNA gene sequences showed that strain LP100(T) occupied a distinct phylogenetic position in the Pontibacter species cluster, showing highest similarity with Pontibacter lucknowensis DM9(T) (97.4 %). Levels of similarity to strains of other Pontibacter species ranged between 94.0 and 96.8 %. Strain LP100(T) contained MK-7 as the predominant menaquinone and sym-homospermidine was the major polyamine in the cell. The major cellular fatty acids of strain LP100(T) were anteiso-C17 : 0 A, iso-C15 : 0 and iso-C18 : 1 H. The polar lipid profile of strain LP100(T) showed the presence of phosphatidylethanolamine, an unidentified aminophospholipid, three unknown aminolipids and two unknown polar lipids. The G+C content of strain LP100(T) was 58.2 mol%. The results of DNA-DNA hybridization, biochemical and physiological tests clearly distinguish the novel strain from closely related species of the genus Pontibacter. Therefore, strain LP100(T) represents a novel species of the genus Pontibacter for which the name Pontibacter indicus is proposed. The type strain is LP100(T) ( = CCM8435(T) = MCC2027(T)).
Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India.
Amit Kumar
AK
Singh
Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India.
Nidhi
N
Garg
Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India.
Pushp
P
Lata
Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India.
Roshan
R
Kumar
Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India.
Vivek
V
Negi
IMTECH-Institute of Microbial Technology, Sector-39A, Chandigarh-160036, India.
Surendra
S
Vikram
Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India.
Rup
R
Lal
D015373:Bacterial Typing Techniques; D001482:Base Composition; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D001556:Hexachlorocyclohexane; D007194:India; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants; D013095:Spermidine / Q000031:analogs & derivatives / Q000737:chemistry; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; C007961:sym-homospermidine; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; D001556:Hexachlorocyclohexane; C062629:menaquinone 7; D013095:Spermidine