Whole-cell screening-based identification of inhibitors against the intraphagosomal survival of Mycobacterium tuberculosis.
We describe here an efficient strategy that employs whole-cell-based screening and further short listing of the compounds by cytotoxicity- and fluorescence-based intracellular assays, resulting in potential bactericidal hits against the growth of Mycobacterium tuberculosis in broth culture as well as in phagosomes. These compounds also inhibited multidrug-resistant strains of M. tuberculosis but showed no or poor inhibition of nonpathogenic mycobacteria or other bacterial species such as Escherichia coli.
Department of Biochemistry, University of Delhi South Campus, New Delhi, India.
Garima
G
Khare
Praveen
P
Kumar
Anil K
AK
Tyagi
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D010588:Phagosomes / Q000382:microbiology
D000900:Anti-Bacterial Agents