Induction of transforming growth factor-beta 1 by androgen is mediated by reactive oxygen species in hair follicle dermal papilla cells.
The progression of androgenetic alopecia is closely related to androgen-inducible transforming growth factor (TGF)-β1 secretion by hair follicle dermal papilla cells (DPCs) in bald scalp. Physiological levels of androgen exposure were reported to increase reactive oxygen species (ROS) generation. In this study, rat vibrissae dermal papilla cells (DP-6) transfected with androgen receptor showed increased ROS production following androgen treatment. We confirmed that TGF-β1 secretion is increased by androgen treatment in DP-6, whereas androgen-inducible TGF-β1 was significantly suppressed by the ROS-scavenger, N-acetyl cysteine. Therefore, we suggest that induction of TGF-β1 by androgen is mediated by ROS in hair follicle DPCs.
Department of Dermatology, Institute of Human-Environment Interface Biology, College of Medicine, Seoul National University, Seoul 110-744, Korea khcho@snu.ac.kr.
Hyoseung
H
Shin
Hyeon Gyeong
HG
Yoo
Shigeki
S
Inui
Satoshi
S
Itami
In Gyu
IG
Kim
A-Ri
AR
Cho
Dong Hun
DH
Lee
Won Seok
WS
Park
Ohsang
O
Kwon
Kwang Hyun
KH
Cho
Chong Hyun
CH
Won
D000111:Acetylcysteine / Q000494:pharmacology; D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D020405:Dermis / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018859:Hair Follicle; D006861:Hydrogen Peroxide / Q000494:pharmacology; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
D000728:Androgens; D017382:Reactive Oxygen Species; D011944:Receptors, Androgen; D053773:Transforming Growth Factor beta1; D006861:Hydrogen Peroxide; D000111:Acetylcysteine