Characterization of cellulose acetates according to DS and molar mass using two-dimensional chromatography.
A two-dimensional liquid chromatographic method (2D LC) was developed to analyze the heterogeneities of cellulose acetates (CA) in the DS-range DS=1.5-2.9 with respect to both, molar mass and degree of substitution (DS). The method uses gradient liquid chromatography (HPLC) as the first dimension in order to separate by DS followed by separation of the different fractions by size (SEC) in the second dimension. The 2D experiments revealed different correlations between gradient and SEC elution volume. These correlations might arise from differences in the synthetic conditions. The newly developed 2D LC separation therefore provides new insights into the heterogeneity of CAs.
Fraunhofer-Institut für Betriebsfestigkeit und Systemzuverlässigkeit LBF, Bereich Kunststoffe, Schlossgartenstraße 6, D-64289 Darmstadt, Germany.
Hewa Othman
HO
Ghareeb
Wolfgang
W
Radke
D002482:Cellulose / Q000031:analogs & derivatives* / Q000302:isolation & purification; D002850:Chromatography, Gel; D002853:Chromatography, Liquid / Q000379:methods*; D005163:Factor Analysis, Statistical; D008970:Molecular Weight
C005062:acetylcellulose; D002482:Cellulose
Cellulose acetate; Degree of substitution; Gradient high performance liquid chromatography; Molar mass; Size exclusion chromatography; Two-dimensional liquid chromatography