Polymorphism of follicle stimulating hormone beta (FSHβ) subunit gene and its association with litter traits in giant panda.
The different SSCP patterns of the follicle stimulating hormone beta (FSHβ) gene amplified by three pairs of primers were sequenced. Comparisons among the three nucleotide sequences of three genotypes indicated that three base substitutions (A213T, A91G, and A89C) were detected in FSHβ gene, which A213T substitution led to one amino acids mutation (Lys > Met), and the other two substitutions were synonymous mutations. The AA, AB and BB genotypes patterns obtained by FSHβ primer1 had evident relation with the litter traits, but the SSCP genotypes patterns obtained by FSHβ primer2 and primer3 had no evident relation with the litter traits in giant panda. The giant panda with AA and AB genotype had the largest litter size and multiparity rate compared with the BB genotypes (P < 0.05). We speculated that the giant pandas with the A allele have better litter traits than those with the B allele.
China Conservation and Research Center for the Giant Panda (CCRCGP), Wolong Nature Reserve, Wenchuan, 623006, Sichuan, People's Republic of China.
Xiaoyu
X
Huang
Desheng
D
Li
Jiwen
J
Wang
Yan
Y
Huang
Chunchun
C
Han
Guiquan
G
Zhang
Zhi
Z
Huang
Honglin
H
Wu
Ming
M
Wei
Guosong
G
Wang
Haiping
H
Hu
Tao
T
Deng
Tao
T
He
Yingming
Y
Zhou
Shixian
S
Song
Bo
B
Luo
Heming
H
Zhang
D000483:Alleles; D000818:Animals; D005260:Female; D037201:Follicle Stimulating Hormone, beta Subunit / Q000235:genetics*; D005787:Gene Frequency; D056726:Genetic Association Studies; D005838:Genotype; D008098:Litter Size / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D019655:Quantitative Trait, Heritable; D017422:Sequence Analysis, DNA; D001503:Ursidae / Q000235:genetics*
D037201:Follicle Stimulating Hormone, beta Subunit