Expression of mesenchymal and α-cell phenotypic markers in islet β-cells in recently diagnosed diabetes.
OBJECTIVE
Relative contributions of reversible β-cell dysfunction and true decrease in β-cell mass in type 2 diabetes remain unclear. Definitive rodent lineage-tracing studies have identified β-cell dedifferentiation and subsequent reprogramming to α-cell fate as a novel mechanism underlying β-cell failure. The aim was to determine whether phenotypes of β-cell dedifferentiation and plasticity are present in human diabetes.


METHODS
Immunofluorescence colocalization studies using classical endocrine and mesenchymal phenotypic markers were undertaken using pancreatic sections and isolated islets from three individuals with diabetes and five nondiabetic control subjects.


RESULTS
Intraislet cytoplasmic coexpression of insulin and vimentin, insulin and glucagon, and vimentin and glucagon were demonstrated in all cases. These phenotypes were not present in nondiabetic control subjects.


CONCLUSIONS
Coexpression of mesenchymal and α-cell phenotypic markers in human diabetic islet β-cells has been confirmed, providing circumstantial evidence for β-cell dedifferentiation and possible reprogramming to α-cells in clinical diabetes.
Corresponding author: James A.M. Shaw, jim.shaw@ncl.ac.uk.
Michael G
MG
White
Helen L
HL
Marshall
Rebecca
R
Rigby
Guo Cai
GC
Huang
Aimen
A
Amer
Trevor
T
Booth
Steve
S
White
James A M
JA
Shaw
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000175:diagnosis* / Q000378:metabolism; D005260:Female; D005934:Glucagon / Q000378:metabolism*; D050416:Glucagon-Secreting Cells / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D008648:Mesoderm / Q000378:metabolism; D008875:Middle Aged; D055815:Young Adult
D015415:Biomarkers; D007328:Insulin; D005934:Glucagon