Adipose tissue-derived stem cells promote pancreatic cancer cell proliferation and invasion.
To explore the effects of adipose tissue-derived stem cells (ADSCs) on the proliferation and invasion of pancreatic cancer cells in vitro and the possible mechanism involved, ADSCs were cocultured with pancreatic cancer cells, and a cell counting kit (CCK-8) was used to detect the proliferation of pancreatic cancer cells. ELISA was used to determine the concentration of stromal cell-derived factor-1 (SDF-1) in the supernatants. RT-PCR was performed to detect the expression of the chemokine receptor CXCR4 in pancreatic cancer cells and ADSCs. An in vitro invasion assay was used to measure invasion of pancreatic cancer cells. SDF-1 was detected in the supernatants of ADSCs, but not in pancreatic cancer cells. Higher CXCR4 mRNA levels were detected in the pancreatic cancer cell lines compared with ADSCs (109.3 ± 10.7 and 97.6 ± 7.6 vs 18.3 ± 1.7, respectively; P<0.01). In addition, conditioned medium from ADSCs promoted the proliferation and invasion of pancreatic cancer cells, and AMD3100, a CXCR4 antagonist, significantly downregulated these growth-promoting effects. We conclude that ADSCs can promote the proliferation and invasion of pancreatic cancer cells, which may involve the SDF-1/CXCR4 axis.
Second Military Medical University, Department of Liver Surgery I, Eastern Hepatobiliary Surgery Hospital, Shanghai, China.
S Q
SQ
Ji
J
J
Cao
Q Y
QY
Zhang
Y Y
YY
Li
Y Q
YQ
Yan
F X
FX
Yu
D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000473:pathology*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D054377:Chemokine CXCL12 / Q000032:analysis*; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009361:Neoplasm Invasiveness / Q000503:physiopathology; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D019718:Receptors, CXCR4 / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000473:pathology / Q000502:physiology*
C527276:CXCR4 protein, human; D054377:Chemokine CXCL12; D017077:Culture Media, Conditioned; D012333:RNA, Messenger; D019718:Receptors, CXCR4