MiR-138 downregulates miRNA processing in HeLa cells by targeting RMND5A and decreasing Exportin-5 stability.
MicroRNAs (miRNAs) are a class of non-coding small RNAs that consist of ≈ 22 nt and are involved in several biological processes by regulating target gene expression. MiR-138 has many biological functions and is often downregulated in cancers. Our results showed that overexpression of miR-138 downregulated target RMND5A (required for meiotic nuclear division 5 homolog A) and reduced Exportin-5 stability, which results in decreased levels of pre-miRNA nuclear export in HeLa cells. We also found that miR-138 could significantly inhibit HeLa cell migration by targeting RMND5A. Our study therefore identifies miR-138-RMND5A-Exportin-5 as a previously unknown miRNA processing regulatory pathway in HeLa cells.
Department of Biochemistry and Molecular Biology, Beijing Institute of Basic Medical Sciences, Beijing 100850, China and Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory for Biocontrol, Sun Yat-sen University, Guangzhou, 510275, China.
Jie
J
Li
Ying
Y
Chen
Xingliang
X
Qin
Junzhi
J
Wen
Hongmei
H
Ding
Wei
W
Xia
Shaohua
S
Li
Xueting
X
Su
Wei
W
Wang
Hui
H
Li
Qiang
Q
Zhao
Tao
T
Fang
Lianghu
L
Qu
Ningsheng
N
Shao
D021581:Active Transport, Cell Nucleus; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002465:Cell Movement; D002467:Cell Nucleus / Q000378:metabolism; D015536:Down-Regulation; D006367:HeLa Cells; D006801:Humans; D028884:Karyopherins / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D055550:Protein Stability; D012322:RNA Precursors / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional
D002352:Carrier Proteins; D028884:Karyopherins; C546110:MIRN138 microRNA, human; D035683:MicroRNAs; C586474:RMND5A protein, human; D012322:RNA Precursors; C446511:XPO5 protein, human