Production of 2-butanol from crude glycerol by a genetically-engineered Klebsiella pneumoniae strain.
Klebsiella pneumoniae was engineered to produce 2-butanol from crude glycerol as a sole carbon source by expressing acetolactate synthase (ilvIH), keto-acid reducto-isomerase (ilvC) and dihydroxy-acid dehydratase (ilvD) from K. pneumoniae, and α-ketoisovalerate decarboxylase (kivd) and alcohol dehydrogenase (adhA) from Lactococcus lactis. Engineered K. pneumonia, ∆ldhA/pBR-iBO (ilvIH–ilvC–ilvD–kivd–adhA), produced 2-butanol (160 mg l−1) from crude glycerol. To increase the yield of 2-butanol, we eliminated the 2,3-butanediol pathway from the recombinant strain by inactivating α-acetolactate decarboxylase (adc). This further engineering step improved the yield of 2-butanol from 160 to 320 mg l−1. This represents the first successful attempt to produce 2-butanol from crude glycerol.
Baek-Rock
BR
Oh
Sun-Yeon
SY
Heo
Sung-Mok
SM
Lee
Won-Kyung
WK
Hong
Jang Min
JM
Park
You Ree
YR
Jung
Dae-Hyuk
DH
Kim
Jung-Hoon
JH
Sohn
Jeong-Woo
JW
Seo
Chul Ho
CH
Kim
D019149:Bioreactors; D000440:Butanols / Q000032:analysis / Q000378:metabolism*; D005285:Fermentation; D005818:Genetic Engineering; D005990:Glycerol / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000235:genetics* / Q000378:metabolism*
D000440:Butanols; C043958:2-butanol; D005990:Glycerol