Optical regulation of protein adsorption and cell adhesion by photoresponsive GaN nanowires.
Interfacing nanowires with living cells is attracting more and more interest due to the potential applications, such as cell culture engineering and drug delivery. We report on the feasibility of using photoresponsive semiconductor gallium nitride (GaN) nanowires (NWs) for regulating the behaviors of biomolecules and cells at the nano/biointerface. The GaN NWs have been fabricated by a facile chemical vapor deposition method. The superhydrophobicity to superhydrophilicity transition of the NWs is achieved by UV illumination. Bovine serum albumin adsorption could be modulated by photoresponsive GaN NWs. Tunable cell detachment and adhesion are also observed. The mechanism of the NW surface responsible for modulating both of protein adsorption and cell adhesion is discussed. These observations of the modulation effects on protein adsorption and cell adhesion by GaN NWs could provide a novel approach toward the regulation of the behaviors of biomolecules and cells at the nano/biointerface, which may be of considerable importance in the development of high-performance semiconductor nanowire-based biomedical devices for cell culture engineering, bioseparation, and diagnostics.
CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology , Beijing 100190, People's Republic of China.
Jingying
J
Li
Qiusen
Q
Han
Ying
Y
Zhang
Wei
W
Zhang
Mingdong
M
Dong
Flemming
F
Besenbacher
Rong
R
Yang
Chen
C
Wang
D000327:Adsorption / Q000187:drug effects; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D005708:Gallium / Q000737:chemistry* / Q000494:pharmacology; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D041681:NIH 3T3 Cells; D036103:Nanotechnology; D053770:Nanowires / Q000737:chemistry*; D012666:Semiconductors; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties
C473348:gallium nitride; D012710:Serum Albumin, Bovine; D005708:Gallium