Gene polymorphisms of Interleukin-4 in allergic rhinitis and its association with clinical phenotypes.
BACKGROUND
Allergic rhinitis (AR) is an inflammatory disorder of the upper airway. T-helper (Th)2 cytokines seems to have major roles behind the scene of unpleasant symptoms resulted from AR. Expression of interleukin (IL)-4 and its receptor could be affected by single nucleotide polymorphisms (SNPs). This study assessed the effect of 4 genetic variants within genes of IL-4 and IL-4R in AR.


METHODS
Allele frequencies of one IL-4R variant (rs1801275) and three SNPs of IL-4 (rs2243248, rs2243250, and rs2070874) were investigated in 98 patients with AR, compared to a group of controls, using PCR sequence-specific-primers (PCR-SSP) method.


RESULTS
Homozygosity for the C allele of rs2243250 in IL-4 was significantly overrepresented in the patient group. CC genotype in rs2070874 significantly was correlated with AR. GG/CC/CC and TT/TT/TT (rs2243248, rs2243250, and rs2070874) haplotypes in the IL-4 gene had a significant negative correlation with AR.


CONCLUSIONS
SNPs in IL-4 are associated with AR and could change the clinical picture of the disease in patients.
Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
Masoud
M
Movahedi
Ali Akbar
AA
Amirzargar
Rasoul
R
Nasiri
Armin
A
Hirbod-Mobarakeh
Elham
E
Farhadi
Marzieh
M
Tavakol
Nasrin
N
Behniafard
Bita
B
Ansaripour
Batoul
B
Moradi
Ahad
A
Zare
Nima
N
Rezaei
D016022:Case-Control Studies; D004804:Eosinophils / Q000378:metabolism; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006239:Haplotypes; D006720:Homozygote; D006801:Humans; D015847:Interleukin-4 / Q000235:genetics*; D008297:Male; D010641:Phenotype; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D019948:Receptors, Interleukin-4 / Q000235:genetics*; D012221:Rhinitis, Allergic, Perennial / Q000235:genetics*; D006255:Rhinitis, Allergic, Seasonal / Q000235:genetics*
D019948:Receptors, Interleukin-4; D015847:Interleukin-4