FoxJ1-expressing cells contribute to neurogenesis in forebrain of adult rats: evidence from in vivo electroporation combined with piggyBac transposon.
Ependymal cells in the lateral ventricular wall are considered to be post-mitotic but can give rise to neuroblasts and astrocytes after stroke in adult mice due to insult-induced suppression of Notch signaling. The transcription factor FoxJ1, which has been used to characterize mouse ependymal cells, is also expressed by a subset of astrocytes. Cells expressing FoxJ1, which drives the expression of motile cilia, contribute to early postnatal neurogenesis in mouse olfactory bulb. The distribution and progeny of FoxJ1-expressing cells in rat forebrain are unknown. Here we show using immunohistochemistry that the overall majority of FoxJ1-expressing cells in the lateral ventricular wall of adult rats are ependymal cells with a minor population being astrocytes. To allow for long-term fate mapping of FoxJ1-derived cells, we used the piggyBac system for in vivo gene transfer with electroporation. Using this method, we found that FoxJ1-expressing cells, presumably the astrocytes, give rise to neuroblasts and mature neurons in the olfactory bulb both in intact and stroke-damaged brain of adult rats. No significant contribution of FoxJ1-derived cells to stroke-induced striatal neurogenesis was detected. These data indicate that in the adult rat brain, FoxJ1-expressing cells contribute to the formation of new neurons in the olfactory bulb but are not involved in the cellular repair after stroke.
Laboratory of Stem Cells and Restorative Neurology, Lund Stem Cell Center, University Hospital, SE-221 84 Lund, Sweden.
Karthikeyan
K
Devaraju
Fanie
F
Barnabé-Heider
Zaal
Z
Kokaia
Olle
O
Lindvall
D000818:Animals; D002454:Cell Differentiation; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D018274:Electroporation; D063928:Ependymoglial Cells / Q000166:cytology / Q000378:metabolism; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D055495:Neurogenesis / Q000235:genetics / Q000502:physiology*; D009830:Olfactory Bulb / Q000166:cytology; D016548:Prosencephalon / Q000166:cytology* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D004251:DNA Transposable Elements; C500666:FOXJ1 protein, rat; D051858:Forkhead Transcription Factors
5′-bromo-2-deoxyuridine; BrdU; Electroporation; Ependymal cells; FoxJ1; GCL; GFAP; GML; LVW; MCAO; NSC; Neurogenesis; OB; SVZ; Stroke; TUNEL; glial fibrillary acidic protein; glomerular layer; granule cell layer; lateral ventricular wall; middle cerebral artery occlusion; neural stem cell; olfactory bulb; subventricular zone; terminal deoxynucleotidyl transferase dUTP nick end labeling