Molecular epidemiology characterization of OXA-23 carbapenemase-producing Acinetobacter baumannii isolated from 8 Brazilian hospitals using repetitive sequence-based PCR.
The typing of multidrug-resistant Acinetobacter baumannii isolates is important for the control and prevention of hospital outbreaks. This study aimed to analyze the molecular epidemiology of 46 OXA-23 carbapenemase-producing A. baumannii strains and compare them to previously described local and international clones (ICs). Isolates were recovered during May 2009-August 2011, from 8 different hospitals in the state of Parana (Brazil). The molecular profiles were determined by repetitive extragenic palindromic PCR. Seven different clusters were identified (A to G). Thirty-two isolates were clustered in the same pattern (clone A), which belong to IC 4.
Department of Microbiology, School of Health and Biosciences, Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil.
Juliette M
JM
Cieslinski
Lavinia
L
Arend
Felipe F
FF
Tuon
Ethianne P
EP
Silva
Rafael G S
RG
Ekermann
Libera Maria
LM
Dalla-Costa
Paul G
PG
Higgins
Harald
H
Seifert
Marcelo
M
Pilonetto
D000151:Acinetobacter Infections / Q000453:epidemiology* / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D001938:Brazil / Q000453:epidemiology; D003428:Cross Infection; D004269:DNA, Bacterial; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D006761:Hospitals; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012091:Repetitive Sequences, Nucleic Acid; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
D004269:DNA, Bacterial; C466679:beta-lactamase OXA-23; D001618:beta-Lactamases
Acinetobacter baumannii; Automated repetitive-sequence-based PCR; DiversiLab; Molecular epidemiology