Dual-sugar imaging using isonitrile and azido-based click chemistries.
We report the first account of metabolically labelling N-acetylglucosamine, in conjunction with either N-acetylgalactosamine or N-acetylmannosamine using a combination of isonitrile- and azide-based chemistries. With the appropriately labelled fluorescent probe molecules, that react with either the azido or isonitrile groups, the method enabled co-visualisation of cancer cell glycoproteins.
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK. fjl1@cam.ac.uk.
Yéléna A
YA
Wainman
André A
AA
Neves
Shaun
S
Stairs
Henning
H
Stöckmann
Heather
H
Ireland-Zecchini
Kevin M
KM
Brindle
Finian J
FJ
Leeper
D000117:Acetylglucosamine / Q000378:metabolism*; D001386:Azides / Q000737:chemistry*; D002460:Cell Line; D057930:Click Chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D057054:Molecular Imaging / Q000379:methods*; D009570:Nitriles / Q000737:chemistry*; D013194:Staining and Labeling
D001386:Azides; D005456:Fluorescent Dyes; D009570:Nitriles; D000117:Acetylglucosamine