Insights into potentially toxic effects of 4-aminoantipyrine on the antioxidant enzyme copper-zinc superoxide dismutase.
4-Aminoantipyrine (AAP) is scarcely administered as an analgesic drug because of side effects. The residue of AAP in the environment is potentially harmful. To evaluate the toxicity of AAP from molecular level, the effects of AAP on the important antioxidant enzyme copper-zinc superoxide dismutase (Cu/ZnSOD) were explored using spectroscopic and molecular modeling methods. AAP can spontaneously bind with Cu/ZnSOD with one binding site to form AAP-Cu/ZnSOD complex through hydrogen bond and van der Waals forces. The molecular docking simulation revealed that AAP bound into the Cu/ZnSOD interface of two subdomains, which induced some conformational and microenvironmental changes of Cu/ZnSOD and further caused the inhibition of Cu/ZnSOD activity. The present study provides important insights into toxic mechanism of AAP with Cu/ZnSOD. The estimated research route can be applied to characterize interactions of enzyme systems and other pollutants and drugs.
School of Environmental and Civil Engineering, Jiangnan University, 1800# Lihu Avenue, Wuxi 214122, PR China.
Yue
Y
Teng
Rutao
R
Liu
D000675:Ampyrone / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002942:Circular Dichroism; D062105:Molecular Docking Simulation; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013482:Superoxide Dismutase / Q000378:metabolism*; D013552:Swine
D000975:Antioxidants; D000675:Ampyrone; D013482:Superoxide Dismutase
Antioxidant enzyme; Docking studies; Multi-spectroscopic techniques; Toxicity evaluation