Influence of FSHR diplotypes on ovarian response to standard gonadotropin stimulation for IVF/ICSI.
OBJECTIVE
To explore the association of FSHR 307 (T/A)/FSHR 680(N/S) diplotypes with ovarian response to follicle stimulating hormone (FSH) stimulation of women undergoing medically assisted reproduction (in vitro fertilization [IVF] or intracytoplasmic sperm injection [ICSI]).


METHODS
The study population consisted of 304 women undergoing IVF/ICSI and 300 women with at least 1 spontaneous pregnancy as controls. FSHR polymorphisms were genotyped. Controlled ovarian stimulation and IVF/ICSI were performed in the 304 couples. During oocyte retrieval the follicular size, the follicle and oocyte numbers were recorded. Serum FSH, luteinizing hormone and estradiol were determined at the third day of the menstrual cycle.


RESULTS
The FSHR 307(T/A)/FSHR 680(N/S) diplotype analysis revealed lower serum FSH levels, higher follicle and oocyte numbers, increased numbers of large follicles as well as decreased empty follicle numbers in Thr307Thr/Asn680Asn women as compared to Thr307 Ala/Asn680Ser and Ala307Ala/Ser680Ser women (p < 0.006, p < 0.01, p < 0.008, p < 0.01, p < 0.005, respectively).


CONCLUSIONS
FSHR diplotypes were significantly associated with ovarian response to gonadotropin stimulation. FSHR diplotype analysis could be informative for ovarian stimulation outcome and the selection of the proper stimulation protocol, which would ensure a sufficient number of mature oocytes for IVF/ICSI.
Genetics and IVF Unit, Department of Obstetrics and Gynecology, University of Ioannina Medical School, 45110 Ioannina, Greece.
Leandros
L
Lazaros
Elissavet
E
Hatzi
Nectaria
N
Xita
Atsushi
A
Takenaka
Nikolaos
N
Sofikitis
Konstantinos
K
Zikopoulos
Ioannis
I
Georgiou
D000328:Adult; D004958:Estradiol / Q000097:blood; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage* / Q000097:blood; D005838:Genotype; D006801:Humans; D007246:Infertility / Q000628:therapy; D007986:Luteinizing Hormone / Q000097:blood; D010062:Ovulation Induction / Q000379:methods*; D011110:Polymorphism, Genetic / Q000235:genetics; D011247:Pregnancy; D011962:Receptors, FSH / Q000235:genetics*; D020554:Sperm Injections, Intracytoplasmic; D016896:Treatment Outcome
D011962:Receptors, FSH; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone