Full genome sequences and preliminary molecular characterization of three tick-borne encephalitis virus strains isolated from ticks and a bank vole in Slovak Republic.
The full genome sequences of three tick-borne encephalitis virus strains, two isolated from Ixodes ricinus ticks and one from the brain of a bank vole, Myodes glareolus, originating from the Slovak Republic were determined. Nucleotide sequences were found to be very similar (>99.5 % nt-identity) with only one distinct amino acid (aa) difference to each other. They all shared 30 aa-changes when compared to type strain Neudoerfl, isolated in neighboring Austria. An internal poly(A)-stretch similar to that of strain Neudoerfl was found only in TBEV strain 114 from a tick. Despite this heterogeneity in the 3'-NCR, the high level of sequence identity was striking, indicating a low rate of nucleotide substitutions of TBEV strains in Slovakia and no obvious relation to the host species.
Bundeswehr Institute of Microbiology, Neuherbergstraße 11, 80937, Munich, Germany.
Stefan
S
Frey
Sandra
S
Essbauer
Gudrun
G
Zöller
Boris
B
Klempa
Gerhard
G
Dobler
Martin
M
Pfeffer
D000818:Animals; D003411:Arvicolinae / Q000821:virology*; D016000:Cluster Analysis; D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D016679:Genome, Viral; D018884:Ixodes / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D018154:Slovakia
D012367:RNA, Viral