Identification of RNA aptamers that internalize into HPV-16 E6/E7 transformed tonsillar epithelial cells.
Human papillomavirus type 16 (HPV-16) associated oropharyngeal cancers are on a significant increase and better therapeutic strategies are needed. The HPV-16 oncogenes E6 and E7 are expressed in HPV-associated cancers and are able to transform human tonsillar epithelial cells (HTECs). We used cell-Systematic Evolution of Ligands by Exponential Enrichment (SELEX) to select for RNA aptamers that entered into HPV-16 E6/E7-HTECs. After 12 rounds of cell-SELEX, a pool of aptamers was obtained that had significantly greater internalization capacity (~5-fold) into E6/E7-HTECs as compared to primary HTECs or fibroblasts. Analysis of individual aptamers from the pool indicated variable internalization into E6/E7-HTECs (1-8-fold as compared to a negative control). Most of the individual aptamers internalized into E6/E7 and primary HTECs with similar efficiency, while one aptamer exhibited ~3-fold better internalization into E6/E7-HTECs. Aptamers that internalize into cells may be useful for delivering therapeutic agents to HPV-16 associated malignancies.
Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA.
Francoise A
FA
Gourronc
William M
WM
Rockey
William H
WH
Thiel
Paloma H
PH
Giangrande
Aloysius J
AJ
Klingelhutz
D052157:Aptamers, Nucleotide / Q000302:isolation & purification / Q000378:metabolism*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism* / Q000821:virology*; D052162:Human papillomavirus 16 / Q000472:pathogenicity*; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D050725:Papillomavirus E7 Proteins / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism*; D052156:SELEX Aptamer Technique
D052157:Aptamers, Nucleotide; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16
Aptamers; HPV; Head neck; Oropharyngeal; Papillomavirus; SELEX