Identification and functional characterization of polyunsaturated fatty acid elongase (McELOVL5) gene from pike eel (Muraenesox cinereus).
The cDNA coding for a polyunsaturated fatty acid elongase (McELOVL5) was isolated from the brain of the pike eel (Muraenesox cinereus) being based on available sequences in 23 types of fish. Four sequence variants were identified with different amino acid substitutions as compared with two clones of McELOVL5 gene (McELOVL5 11.7 and McELOVL5 12.4). When the two variants of McELOVL5 were expressed in Saccharomyces cerevisiae, the two recombinant yeasts elongated γ-linolenic acid (GLA, 18:3n-6) to di-homo-γ-linolenic acid (DGLA, 20:3n-6) but differed in the rate of GLA conversion to DGLA. Cells transformed with McELOVL5 12.4 also converted arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3) to docosatetraenoic acid (22:4n-6) and docosapentaenoic acid (22:5n-3), respectively. However McELOVL5 11.7 lost its function for the elongation of C20 fatty acids. The four sequence variants have changed substrate specificities. Three-dimensional models of the McELOVL5 proteins are suggested.
Sun Hee
SH
Kim
So Yun
SY
Kim
Eui Ki
EK
Kim
Kyung Hee
KH
Roh
Jung-Bong
JB
Kim
Kwang-Soo
KS
Kim
Hyun Uk
HU
Kim
Kyeong-Ryeol
KR
Lee
Han-Chul
HC
Kang
Jong-Sug
JS
Park
Kyung Hyun
KH
Kim
Jong-Bum
JB
Kim
D000123:Acetyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D003001:Cloning, Molecular; D004524:Eels / Q000235:genetics*; D000081031:Fatty Acid Elongases; D005227:Fatty Acids / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
D005227:Fatty Acids; D011994:Recombinant Proteins; D012313:RNA; D000123:Acetyltransferases; D000081031:Fatty Acid Elongases