Genetic diversity of MYH3 gene associated with growth and carcass traits in Chinese Qinchuan cattle.
MYH3, whose function is to convert chemical energy to mechanical energy through ATP hydrolysis, is mainly expressed in skeletal muscle at various stages and is indispensable in the procedure of development of skeletal muscle and heart. In the study, genetic variations and genotypes of MYH 3 gene in a total of 365 Qinchuan cattles were analyzed by polymerase chain reaction-restriction fragment length polymorphism, as well as verified the effect on growth and carcass traits. After PCR products were digested by restriction enzymes, eight SNPs were identified and individuals were genotyped. It showed that the SNPs at nucleotides were all in low linkage disequilibrium, therefore no dominated haplotype was found in the population. The result of statistic analysis indicated seven SNPs were significantly associated with growth and carcass traits (P < 0.05, N = 365) except locus G13791A. To sum up, the result of the study proved that polymorphisms in MYH3 gene are associated with the growth performance of Chinese Qinchuan cattle, so the variations of the gene could be used as possible molecular assisted-makers in the beef cattle breeding program and management.
College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
Fubiao
F
Niu
Lijun
L
Wang
Xiaolin
X
Liu
Hongliang
H
Wang
Jing
J
Yang
Yu
Y
Liu
Ling
L
Chen
D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000254:growth & development*; D002681:China; D003598:Cytoskeletal Proteins / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D014644:Genetic Variation; D015810:Linkage Disequilibrium / Q000235:genetics; D008460:Meat; D009154:Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D019655:Quantitative Trait, Heritable
D003598:Cytoskeletal Proteins