A proteomic approach of pediatric astrocytomas: MiRNAs and network insight.
Pediatric astrocytomas, a leading cause of death associated with cancer, are the most common primary central nervous system tumors found in children. Most studies of these tumors focus on adults, not on children. We examined the global protein and microRNA expression pattern by 2D SDS-PAGE, mass spectrometry (MALDI-TOF), and RT(2) miRNA PCR Array System. Proteomic studies revealed 49 proteins with changes on the expression. Interactome showed that vimentin, calreticulin, and 14-3-3 epsilon protein are hub proteins in these neoplasms. MicroRNA analyses demonstrated for the first time novel microRNAs involved in the astrocytoma biology. In conclusion, our results show that novel proteins and microRNAs with expression changes on pediatric astrocytoma could serve as biomarkers of tumor progression.


CONCLUSIONS
Astrocytomas are tumors that progress rapidly and that invade surrounding tissues. Although some drugs have been developed to treat these neoplasms, the mortality of patients is still very high. In this study, we describe for the first time, to our knowledge, some proteins and miRNAs associated with the biology of astrocytic tumors that could be postulated as possible diagnostic or prognostic biomarkers. Altogether, our results indicate that large-scale analyses allow making a fairly accurate prediction of different cellular processes altered in astrocytic tumors.
Unidad de Investigación Médica en Genética Humana, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, IMSS, 06720 México, D.F., Mexico; Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México, Av. Universidad 3000, C.P. 04510 Coyoacán, D. F., Mexico.
Ruth
R
Ruiz Esparza-Garrido
Miguel Á
MÁ
Velázquez-Flores
Jaime
J
Diegopérez-Ramírez
Enrique
E
López-Aguilar
Georgina
G
Siordia-Reyes
Magdalena
M
Hernández-Ortiz
Angel G
AG
Martínez-Batallar
Sergio
S
Encarnación-Guevara
Fabio
F
Salamanca-Gómez
Diego Julio
DJ
Arenas-Aranda
D000328:Adult; D001254:Astrocytoma / Q000378:metabolism* / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D020543:Proteome / Q000096:biosynthesis*; D040901:Proteomics; D012334:RNA, Neoplasm / Q000096:biosynthesis*
D035683:MicroRNAs; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D020543:Proteome; D012334:RNA, Neoplasm
Hub proteins; Novel miRNAs; Pediatric astrocytoma; Protein–protein interactions; Proteomic