Proteomic analysis in usual and nonspecific interstitial pneumonia.
Differentiating nonspecific interstitial pneumonia (NSIP) from usual interstitial pneumonia (UIP) is important for the determination of both treatment and prognosis. Using two-dimensional fluorescence difference gel electrophoresis (2D-DIGE), we examined 8 UIPs, 8 NSIPs, and 30 normal lung tissues. Comparisons with control in 2D-DIGE showed that (a) in UIP, nine protein spots were significantly upregulated and seven were significantly downregulated, (b) in NSIP, four protein spots were significantly upregulated and nine were significantly downregulated. The detected proteins were analyzed by MALDI-TOF mass spectrometry, allowing qualitative differences in vimentin subtypes to be characterized. One vimentin subtype was upregulated in UIP, while another one was downregulated in NSIP (vs. control). These different characteristics were partially supported by the results of Western blot analysis. Our immunohistochemistry revealed vimentin expression within fibroblasts (a) in fibroblastic foci in UIP and (b) in fibrotic alveolar walls in NSIP. Differences in vimentin subtypes may provide useful biomarkers for separating NSIP from UIP, alongside differences in histological characteristics.
Department of Laboratory Medicine, National Defense Medical College Hospital, Tokorozawa, Japan.
Ichiyo
I
Ohara
Department of Pathology, Mita Hospital, International University of Health and Welfare, Tokyo, Janpan.
Shinsuke
S
Aida
Department of Laboratory Medicine, National Defense Medical College Hospital, Tokorozawa, Japan.
Hideyuki
H
Shimazaki
Department of Respiratory Medicine, National Defense Medical College, Tokorozawa, Japan.
Hideo
H
Kobayashi
Department of Basic Pathology, National Defense Medical College, Tokorozawa. Japan.
Hitoshi
H
Tsuda
Department of Gene Regulation and Protein Function, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.
Tosifusa
T
Toda
Department of Laboratory Medicine, National Defense Medical College Hospital, Tokorozawa, Japan. nknsknak@ndmc.ac.jp.
Kuniaki
K
Nakanishi
Department of Laboratory Medicine, National Defense Medical College Hospital, Tokorozawa, Japan.
Seiichi
S
Tamai
D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D003937:Diagnosis, Differential; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D054990:Idiopathic Pulmonary Fibrosis / Q000175:diagnosis* / Q000378:metabolism; D007150:Immunohistochemistry; D017563:Lung Diseases, Interstitial / Q000175:diagnosis / Q000378:metabolism; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014746:Vimentin / Q000096:biosynthesis*
D015415:Biomarkers; D014746:Vimentin