Highly efficient bienzyme functionalized nanocomposite-based microfluidics biosensor platform for biomedical application.
This report describes the fabrication of a novel microfluidics nanobiochip based on a composite comprising of nickel oxide nanoparticles (nNiO) and multiwalled carbon nanotubes (MWCNTs), as well as the chip's use in a biomedical application. This nanocomposite was integrated with polydimethylsiloxane (PDMS) microchannels, which were constructed using the photolithographic technique. A structural and morphological characterization of the fabricated microfluidics chip, which was functionalized with a bienzyme containing cholesterol oxidase (ChOx) and cholesterol esterase (ChEt), was accomplished using X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy. The XPS studies revealed that 9.3% of the carboxyl (COOH) groups present in the nNiO-MWCNT composite are used to form amide bonds with the NH2 groups of the bienzyme. The response studies on this nanobiochip reveal good reproducibility and selectivity, and a high sensitivity of 2.2 mA/mM/cm2. This integrated microfluidics biochip provides a promising low-cost platform for the rapid detection of biomolecules using minute samples.
1] Department of Science and Technology Centre on Biomolecular Electronics, Biomedical Instrumentation Section, CSIR-National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi-110012, India [2] Indian Institute of Technology Hyderabad, Ordnance Factory Estate, Yeddumailaram, Hyderabad, Andhra Pradesh 502205, India.
Md Azahar
MA
Ali
Saurabh
S
Srivastava
Pratima R
PR
Solanki
Venu
V
Reddy
Ved V
VV
Agrawal
CheolGi
C
Kim
Renu
R
John
Bansi D
BD
Malhotra
D015374:Biosensing Techniques / Q000295:instrumentation; D002789:Cholesterol Oxidase / Q000737:chemistry / Q000378:metabolism; D004566:Electrodes; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D044085:Microfluidics / Q000295:instrumentation; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000737:chemistry / Q000648:ultrastructure; D009532:Nickel / Q000737:chemistry; D056951:Photoelectron Spectroscopy; D013059:Spectrum Analysis, Raman; D002787:Sterol Esterase / Q000737:chemistry / Q000378:metabolism; D014961:X-Ray Diffraction
D004798:Enzymes; D037742:Nanotubes, Carbon; D009532:Nickel; C028007:nickel monoxide; D002789:Cholesterol Oxidase; D002787:Sterol Esterase