Comparison of TLC and Different Micro TLC Techniques in Analysis of Tropane Alkaloids and Their Derivatives Mixture fromDatura InoxiaMill. Extract.
Planar chromatography is a very useful tool for analysis of wide range of different mixtures. Thanks to its possibility for rapid separation of large number of samples simultaneously, low solvent consumption and ability to analyse rough material allow to receive precise and reliable results in short time and low cost. Miniaturization of planar techniques brings a lot of advantages, such as shortening distance and time of chromatogram development, and further lowering of solvent consumption. Besides, it often allows to improve separation parameters and raise efficiency of chromatographic system. In this paper, ability of analysis of tropane alkaloids mixture fromDatura InoxiaMill. extract using conventional TLC technique with five micro TLC techniques (short distance TLC, HPTLC, UTLC, OPLC and ETLC) in maximally closed chromatographic conditions was compared in order to present abilities of micro TLC techniques in plant material analysis.
Department of Planar Chromatography, Faculty of Chemistry, Maria Curie-Skłodowska University, Lublin, Poland.
Irena
I
Malinowska
Marek
M
Studziński
Karolina
K
Niezabitowska
Maria
M
Gadzikowska
ETLC; HPTLC; TLC; Tropane alkaloids; UTLC