Impact of ZnO embedded feed spacer on biofilm development in membrane systems.
The concept of suppressing biofouling formation using an antibacterial feed spacer was investigated in a bench scale-cross flow system mimicking a spiral wound membrane configuration. An antibacterial composite spacer containing zinc oxide-nanoparticles was constructed by modification of a commercial polypropylene feed spacer using sonochemical deposition. The ability of the modified spacers to repress biofilm development on membranes was evaluated in flow-through cells simulating the flow conditions in commercial spiral wound modules. The experiments were performed at laminar flow (Re = 300) with a 200 kDa molecular weight cut off polysulfone ultrafiltration membrane using Pseudomonas putida S-12 as model biofilm bacteria. The modified spacers reduced permeate flux decrease at least by 50% compared to the unmodified spacers (control). The physical properties of the modified spacer and biofilm development were evaluated using high resolution/energy dispersive spectrometry-scanning electron microscopy, atomic force microscopy and confocal laser scanning microscopy imaging (HRSEM, EDS, AFM and CLSM). HRSEM images depicted significantly less bacteria attached to the membranes exposed to the modified spacer, mainly scattered and in a sporadic monolayer structure. AFM analysis indicated the influence of the modification on the spacer surface including a phase change on the upper surface. Dead-live staining assay by CLSM indicated that most of the bacterial cells attached on the membranes exposed to the modified spacer were dead in contrast to a developed biofilm which was predominant in the control samples.
Civil and Environmental Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel; Grand Water Research Institute, Technion-Israel Institute of Technology, Haifa 32000, Israel.
Avner
A
Ronen
Raphael
R
Semiat
Carlos G
CG
Dosoretz
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D057219:Biofouling; D008567:Membranes, Artificial; D008826:Microbial Sensitivity Tests; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D011108:Polymers / Q000494:pharmacology; D011126:Polypropylenes / Q000494:pharmacology; D016958:Pseudomonas putida / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D013450:Sulfones / Q000494:pharmacology; D014462:Ultrafiltration; D015032:Zinc / Q000302:isolation & purification; D015034:Zinc Oxide / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D008567:Membranes, Artificial; D011108:Polymers; D011126:Polypropylenes; D013450:Sulfones; C017662:polysulfone P 1700; D015032:Zinc; D015034:Zinc Oxide
Biofouling; Feed spacer; Membrane separation; Water treatment; Zinc oxide nanoparticles