Aging of biological matrices and its effect on bioanalytical method performance.
Apart from the well-known matrix effects that can occur in ESI LC-MS, biological matrices may have other effects influencing the quantitative reliability of bioanalytical methods. In this paper, six case studies are presented that show the effect that aging, that is the change in properties and composition of biological matrices over time, can have on the performance of bioanalytical methods. It is shown that selectivity can be affected due to the formation or disappearance of endogenous compounds. Stability can be influenced because of the decrease (or increase) of enzyme activities and recovery can be impacted if the extractability from binding sites in the matrix is enhanced or decreased. A general discussion on the importance of these matrix effects is provided as well as a perspective on how to properly address them in the method-development and validation stages of regulated bioanalysis.
PRA, Bioanalytical Laboratory, Westerbrink 3, 9405 BJ Assen, The Netherlands. merbelnicovande@praintl.com.
Nico C
NC
van de Merbel
Ronald
R
de Vries
D000818:Animals; D002784:Cholesterol / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D016572:Cyclosporine / Q000097:blood; D003561:Cytarabine / Q000097:blood; D006801:Humans; D006888:Hydroxycholesterols / Q000097:blood; D007553:Isotope Labeling; D051379:Mice; D010084:Oxidation-Reduction; D044967:Serum / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature; D013997:Time Factors; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000097:blood
D006888:Hydroxycholesterols; D003561:Cytarabine; D014451:Ubiquinone; C099828:cholest-5-ene-3,4-diol; D016572:Cyclosporine; D002784:Cholesterol; C024989:coenzyme Q10; C030777:ubiquinone 7