Control of oxidative reactions of hemoglobin in the design of blood substitutes: role of the Vc, NAC, TEMPO and their reductant system.
Oxidative reactions of hemoglobin (Hb) are still a serious problem for Hb-based blood substitute development. Although varieties of antioxidant strategies have been suggested, this in vitro study examined the ability of the ascorbate, N-Acetyl-L-Cysteine (NAC), 4-hydroxy-2, 2, 6, 6-tetramethylpiperidine-1-oxygen free radicals (TEMPO) and their reductant system in preventing Hb oxidation. The content of ferric Hb is monitored in the process of vitamin C (Vc), NAC, TEMPO and their reductant system. The results suggest that ascorbate is effective in reducing ferryl Hb, and TEMPO with Vc/NAC could obviously shorten the reaction time, but it does not play the role of Met-Hb reductases. It demonstrates that TEMPO did little to recover Hb under oxidative stress.
School of Environment and Chemical Engineering, Tianjin Polytechnic University , Tianjin , P. R. China.
Xiulan
X
Su
Song
S
Guo
Xiaoxia
X
Huang
Xiang
X
Wang
Donglai
D
Qi
Chengmin
C
Yang
D000111:Acetylcysteine / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D001802:Blood Substitutes / Q000378:metabolism*; D003497:Cyclic N-Oxides / Q000494:pharmacology*; D005260:Female; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D010084:Oxidation-Reduction / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism; D011247:Pregnancy
D000975:Antioxidants; D001802:Blood Substitutes; D003497:Cyclic N-Oxides; D006454:Hemoglobins; D001205:Ascorbic Acid; D010100:Oxygen; C003959:TEMPO; D000111:Acetylcysteine
Met-Hb; NAC; TEMPO; Vc; reduction