Chemical constituents from the roots of Cynanchum paniculatum and their cytotoxic activity.
Two new steroidal saponins, cynanside A (1) and B (2) were isolated together with three known compounds (3-5) from the roots of Cynanchum paniculatum (Bunge) Kitag. The structures of 1 and 2 were elucidated on the basis of spectroscopic analyses, including extensive 2D NMR and acid hydrolysis. We evaluated the cytotoxicity of isolates (1-5) against A549, SK-OV-3, SK-MEL-2, and HCT-15 cell lines in vitro using the SRB bioassay. Compounds 1 and 2 showed selective cytotoxicity against SK-MEL-2 cells with IC50 values of 26.55 and 17.36μM, respectively.
Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Chung Sub
CS
Kim
Ju Yeoun
JY
Oh
Sang Un
SU
Choi
Kang Ro
KR
Lee
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D031105:Cynanchum / Q000737:chemistry*; D003603:Cytotoxins / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008968:Molecular Conformation; D018517:Plant Roots / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D013329:Structure-Activity Relationship
D000972:Antineoplastic Agents, Phytogenic; D003603:Cytotoxins; D012503:Saponins; C000591619:cynanside A; C000591620:cynanside B
Asclepiadaceae; Cynanchum paniculatum; Cytotoxicity; Steroidal glycosides