Identification and characterization of FaSOC1, a homolog of SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 from strawberry.
A MADS-box gene SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) integrates multiple flowering signals to regulate floral transition in Arabidopsis. Strawberry (Fragaria spp.) is an economically important fruit crop, but its molecular control of flowering is largely unknown. In this study, a SOC1-like gene, FaSOC1, was isolated and characterized from strawberry. The open reading frame of FaSOC1 was 648bp, encoding a protein of 215 amino acids. Sequence alignment and phylogenetic analysis showed that the FaSOC1 protein contained a highly conserved MADS domain and a SOC1 motif, and that it was a member of the SOC1-like genes of dicots. The FaSOC1 protein mainly localized in the cytoplasm of onion epidermal cells and Arabidopsis protoplasts, and showed no transcriptional activation activity in yeast cells. Under the floral induction conditions, the expression of FaSOC1 increased during the first 2weeks of short-day treatment, but declined dramatically during three to 4weeks. FaSOC1 was highly expressed in reproductive organs, including shoot apices, floral buds, flowers, stamens and sepals. Overexpression of FaSOC1 in wild-type Arabidopsis caused early flowering and upregulated the expression of flowering time genes LFY and AP1. In addition, the yeast two-hybrid and BiFC assays confirmed that FaSOC1 could interact with AGL24. In conclusion, these results suggest that FaSOC1 is a flowering promoter in strawberry.
Department of Fruit Science, College of Agriculture and Biotechnology, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, People's Republic of China.
Heng-Jiu
HJ
Lei
Hua-Zhao
HZ
Yuan
Yun
Y
Liu
Xin-Wei
XW
Guo
Xiong
X
Liao
Lin-Lin
LL
Liu
Qi
Q
Wang
Tian-Hong
TH
Li
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D035264:Flowers / Q000235:genetics / Q000378:metabolism; D031985:Fragaria / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D026161:MADS Domain Proteins / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification; D030821:Plants, Genetically Modified; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014018:Tissue Distribution
C419046:AGL20 protein, Arabidopsis; D029681:Arabidopsis Proteins; D026161:MADS Domain Proteins; D010940:Plant Proteins
Arabidopsis; CaMV; DAG; FaSOC1; Flowering; LD; MADS-box gene; ORF; SD; Strawberry; WSD; cauliflower mosaic virus; days after germination; long-day; open reading frame; qRT-PCR; quantitative real-time PCR; short-day; weeks of SD treatment