Identification and characterization of ethanol utilizing fungal flora of oil refinery contaminated soil.
The indigenous fungal flora of three oil refinery contaminated sites (Bharuch, Valsad and Vadodara) of India has been documented in the present investigation. A total seventy-five fungal morphotypes were isolated from these sites and out of them, only fifteen isolates were capable of utilizing ethanol (0-8%; v:v) as a sole source of carbon and energy for growth. Ten percent ethanol was completely lethal for the growth of all the isolated fungus. Biochemical characterization of the potent ethanol utilizing fungal isolates was studied based on substrate utilization profiles using BIOLOG phenotype microarray plates. Based on the morphological characters and Internal Transcribed Spacer region of ribosomal DNA, the fungal isolates were identified as Fusarium brachygibbosum, Fusarium equiseti, Fusarium acuminatum, Pencillium citrinum, Alternaria tenuissima, Septogloeum mori, Hypocrea lixii, Aureobasidium sp., Penicillium sp., and Fusarium sp. Intra-species genetic diversity among Fusarium sp. was evaluated by whole genome analysis with repetitive DNA sequences (ERIC, REP and BOX) based DNA fingerprinting. It was found that these fungus use alcohol dehydrogenase and acetaldehyde dehydrogenase enzymes based metabolism pathway to utilize ethanol for their growth and colonization.
National Bureau of Agriculturally Important Microorganisms (NBAIM), Mau, 275101, Uttar Pradesh, India.
Alok Kumar
AK
Srivastava
Pratiksha
P
Singh
Rajesh Kumar
RK
Singh
Prem Lal
PL
Kashyap
Hillol
H
Chakdar
Sudheer
S
Kumar
Arun Kumar
AK
Sharma
D044822:Biodiversity; D002244:Carbon / Q000378:metabolism; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004734:Energy Metabolism; D000431:Ethanol / Q000378:metabolism*; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D007194:India; D008969:Molecular Sequence Data; D058889:Molecular Typing; D016533:Mycological Typing Techniques; D009821:Oils; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D009821:Oils; D012989:Soil Pollutants; D000431:Ethanol; D002244:Carbon