DNA detection using plasmonic enhanced near-infrared photoluminescence of gallium arsenide.
Efficient near-infrared detection of specific DNA with single nucleotide polymorphism selectivity is important for diagnostics and biomedical research. Herein, we report the use of gallium arsenide (GaAs) as a sensing platform for probing DNA immobilization and targeting DNA hybridization, resulting in ∼8-fold enhanced GaAs photoluminescence (PL) at ∼875 nm. The new signal amplification strategy, further coupled with the plasmonic effect of Au nanoparticles, is capable of detecting DNA molecules with a detection limit of 0.8 pM and selectivity against single base mismatches. Such an ultrasensitive near-infrared sensor can find a wide range of biochemical and biomedical applications.
Department of Chemistry, Beijing Key Laboratory for Analytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University , Beijing, China 100084.
Longhua
L
Tang
Ik Su
IS
Chun
Zidong
Z
Wang
Jinghong
J
Li
Xiuling
X
Li
Yi
Y
Lu
D001152:Arsenicals / Q000737:chemistry*; D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D005708:Gallium / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D007259:Infrared Rays; D057230:Limit of Detection; D008163:Luminescent Measurements; D053768:Metal Nanoparticles / Q000737:chemistry; D009693:Nucleic Acid Hybridization
D001152:Arsenicals; C043055:gallium arsenide; D006046:Gold; D004247:DNA; D005708:Gallium