Analysis of PTP1B sumoylation.
Signaling enzymes are typically regulated by a variety of post-translational modifications. One such enzyme, protein tyrosine phosphatase (PTP) 1B, which plays an important role in metabolic and growth signaling, has recently been shown to be inhibited by posttranslational modifications by ubiquitin-related proteins of the SUMO family. We have adapted several approaches to analyzing the sites and effects of sumoylation of PTP1B in cells and in vitro. The principal outline of the assays should be applicable to other enzymes as well.
Cancer Biology Program, Fox Chase Cancer Center, Philadelphia, PA, United States.
Sayanti
S
Saha
Cancer Biology Program, Fox Chase Cancer Center, Philadelphia, PA, United States. Electronic address: Jonathan.Chernoff@fccc.edu.
Jonathan
J
Chernoff
D001120:Arginine / Q000737:chemistry; D001681:Biological Assay / Q000379:methods*; D002478:Cells, Cultured; D008239:Lysine / Q000737:chemistry; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000737:chemistry / Q000378:metabolism*; D058207:Sumoylation
D001120:Arginine; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D008239:Lysine
Phosphorylation; Posttranslational modification; Protein tyrosine phosphatases; Signal transduction; Sumoylation