Simultaneous determination of amino acids and carbohydrates in culture media of Clostridium thermocellum by valve-switching ion chromatography.
An improved method for the simultaneous determination of 20 amino acids and 7 carbohydrates using one-valve switching after injection, ion chromatography, and integrated pulsed amperometric detection is proposed. The resolution of the amino acids and carbohydrates in the cation trap column was investigated. In addition, parameters including flow liquid type, flow rate, concentration, and valve-switch timing were optimized. The method is time-saving, effective, and accurate for the simultaneous separation of amino acids and carbohydrates, with a mean correlation coefficient of >0.99 and repeatability of 0.5-4.6% for eight replicates. The method was successfully applied in the analysis of amino acids and carbohydrates in aseptic media and in extracellular culture media of three phenotypes of Clostridium thermocellum.
Public Laboratory of Bioenergy and Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao 266101, China. Electronic address: fayun@qibebt.ac.cn.
Yun
Y
Fa
Haiyan
H
Yang
Chengshuai
C
Ji
He
H
Cui
Xinshu
X
Zhu
Juan
J
Du
Jun
J
Gao
D000596:Amino Acids / Q000032:analysis* / Q000302:isolation & purification; D002241:Carbohydrates / Q000032:analysis* / Q000302:isolation & purification; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation; D048013:Acetivibrio thermocellus / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D055664:Electrochemical Techniques; D013696:Temperature
D000596:Amino Acids; D002241:Carbohydrates; D003470:Culture Media
Amino acids; Carbohydrates; Electrochemical detector; Ion chromatography; Valve switching