[Effects of Fukang oral liquid on the prevention of intrauterine adhesion and expressions of TGF-beta1, PAI-1 and MMP-9 in endometrium of rats].
OBJECTIVE
To investigate the preventing effect of Fukang oral liquid (fuk) in intrauterine adhesions and its effects on the expression of TGFbeta-1, PAI-1 and MMP-9 in endometrium of rats with intrauterine adhesions.


METHODS
50 female wistar rats were divided into high, medium, low dose of Fukang oral liquid group (Hfuk, Mfuk, Lfuk), blank control group (Bcon), and model control group (Mcon) (n = 10 in each group). The rats in Hfuk, Mfuk and Lfuk groups were treated with intragastric administration of 4 mL, 2 mL and 1 mL Fukang Oral Liquid per day, while the rats in Mcon group and Bcon group received 2 mL physiological saline intragastric administration per day. All of rats were executed on 10th day and the sample of endometrium was harvested for the study of histology and morphology and the expression of TGFbeta-1, PAI-1 and MMP-9.


RESULTS
Under the light microscope, the organizational structure of the uterine cavity and uterine wall was clear in Bcon group, the uterine cavity disappeared in Mcon group, and the layers structure remained normal arrangement in three fuk treated groups. TGFbeta-1 and PAI-1 protein expressions in Hfuk, Mfuk, Lfuk groups were less than those in Mcon group (P < 0.001), but MMP-9 protein expressions were higher. (P < 0.001).


CONCLUSIONS
Fukang oral liquid show preventing effect on IUA, the mechanism may be related to its effects on the expressions of TGF-beta1, PAI-1, and MMP-9 in the endometrium.
Department of Gynaecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Sha
S
Hu
Ya
Y
Li
Wei-Jie
WJ
Meng
Shi-Qiao
SQ
Tan
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D000267:Tissue Adhesions / Q000188:drug therapy* / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D014591:Uterine Diseases / Q000188:drug therapy* / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D017395:Plasminogen Activator Inhibitor 1; C547314:Serpine1 protein, rat; C508962:Tgfb1 protein, rat; D053773:Transforming Growth Factor beta1; D020780:Matrix Metalloproteinase 9; C572337:Mmp9 protein, rat