Influence of glucosamine on oligochitosan solubility and antibacterial activity.
Light scattering studies indicate that oligochitosan (short-chain chitosan) solutions contain aggregates at pH values below the critical pH of phase separation, while at or above this point the gel phase coexists with the aggregate solution. This work demonstrates for the first time that the presence of D-glucosamine in an oligochitosan solution shifts the critical pH to a higher value and improves the oligochitosan antibacterial activity against Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermis in neutral and slightly alkaline aqueous media. By comparing the results of light scattering studies and antimicrobial assays one can conclude that the antimicrobial activity of oligochitosan is dependent on its unimolecular form, not its supramolecular structures. The widening of the homogeneity region of an oligochitosan solution could lead to promising biomedical applications.
A.N. Nesmeyanov Institute of Organoelement Compounds of the Russian Academy of Sciences, V-334, Vavilov st. 28, Moscow 119991, Russia.
Inesa V
IV
Blagodatskikh
Sergey N
SN
Kulikov
Oxana V
OV
Vyshivannaya
Evgeniya A
EA
Bezrodnykh
Igor A
IA
Yamskov
Vladimir E
VE
Tikhonov
D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D002686:Chitin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D048271:Chitosan; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects; D005944:Glucosamine / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D009844:Oligosaccharides; D012995:Solubility / Q000187:drug effects; D013210:Staphylococcus / Q000187:drug effects; D013329:Structure-Activity Relationship
D000900:Anti-Bacterial Agents; D009844:Oligosaccharides; C493484:oligochitosan; D002686:Chitin; D048271:Chitosan; D005944:Glucosamine
Antibacterial activity; E. coli; Glucosamine; Oligochitosan; S. aureus; S. epidermidis