A DNA vaccine targeting p42.3 induces protective antitumor immunity via eliciting cytotoxic CD8+T lymphocytes in a murine melanoma model.
The p42.3 gene was recently identified and characterized as having tumor-specific and mitosis phase-dependent expression in many types of cancer. This suggested that p42.3 antigen could be used as a target for vaccines against cancers. In this study, we immunized C57BL/6 mice with a DNA vaccine encoding p42.3. We used intramuscular injection with electroporation, either before or after challenge with tumor B16F10 cells. Vaccination with pcDNA3-p42.3 induced some degree of antitumor effect both therapeutically and prophylactically, as evaluated by the inhibition of tumor growth and decrease in tumor weight. Immunized mice showed a high level of specific cytotoxic activity against the p42.3 protein in vivo and had activated CD8 T cells that secreted IFN-γ, perforin, and granzyme B in response to stimulation with the antigen in vitro. Thus, this study presents the DNA vaccination against novel tumor target p42.3 as a promising antitumor modality.
State Key Laboratory for Agro-Biotechnology; College of Biological Science; China Agricultural University; Beijing, P.R. China.
Hu
H
Liu
Key Laboratory of Medical Molecular Virology of MOH and MOE; Fudan University Shanghai Medical College; Shanghai, P.R. China.
Shuang
S
Geng
State Key Laboratory for Agro-Biotechnology; College of Biological Science; China Agricultural University; Beijing, P.R. China.
Congcong
C
Feng
State Key Laboratory for Agro-Biotechnology; College of Biological Science; China Agricultural University; Beijing, P.R. China.
Xiaoping
X
Xie
State Key Laboratory for Agro-Biotechnology; College of Biological Science; China Agricultural University; Beijing, P.R. China.
Bing
B
Wu
State Key Laboratory for Agro-Biotechnology; College of Biological Science; China Agricultural University; Beijing, P.R. China.
Xuan
X
Chen
State Key Laboratory for Agro-Biotechnology; College of Biological Science; China Agricultural University; Beijing, P.R. China.
Qiang
Q
Zou
State Key Laboratory for Agro-Biotechnology; College of Biological Science; China Agricultural University; Beijing, P.R. China.
Shuang
S
Wang
Laboratory of Molecular Oncology; Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education); Peking University Cancer Hospital & Institute; Beijing, P.R. China.
Jiantao
J
Cui
Laboratory of Molecular Oncology; Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education); Peking University Cancer Hospital & Institute; Beijing, P.R. China.
Rui
R
Xing
Laboratory of Molecular Oncology; Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education); Peking University Cancer Hospital & Institute; Beijing, P.R. China.
Wenmei
W
Li
Laboratory of Molecular Oncology; Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education); Peking University Cancer Hospital & Institute; Beijing, P.R. China.
Youyong
Y
Lu
State Key Laboratory for Agro-Biotechnology; College of Biological Science; China Agricultural University; Beijing, P.R. China; Key Laboratory of Medical Molecular Virology of MOH and MOE; Fudan University Shanghai Medical College; Shanghai, P.R. China.
Bin
B
Wang
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D018274:Electroporation; D005260:Female; D053804:Granzymes / Q000378:metabolism; D007273:Injections, Intramuscular; D007371:Interferon-gamma / Q000378:metabolism; D008545:Melanoma / Q000276:immunology* / Q000517:prevention & control / Q000628:therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D054353:Perforin / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
D019496:Cancer Vaccines; D018797:Cell Cycle Proteins; D019444:Vaccines, DNA; D054353:Perforin; D007371:Interferon-gamma; D053804:Granzymes
B16F10 melanoma; DNA vaccine; cancer immunotherapy; cytotoxic CD8+T cell; p42.3