Effect of icariin on cell proliferation and the expression of bone resorption/formation-related markers in human periodontal ligament cells.
Periodontitis is a common destructive inflammatory disease that leads to changes in the tooth-supporting tissues. Human periodontal ligament cells are essential in periodontal tissue regeneration. The traditional Chinese medicine icariin promoted bone formation, stimulated the osteogenic differentiation of preosteoblastic cells and inhibited osteoclast differentiation and bone resorption. Thus, in the present study, the effect of icariin on cell proliferation and the expression of osteoprotegerin (OPG), receptor activator of nuclear factor-κB ligand (RANKL), core binding factor α1 (Cbfa1) and osteocalcin (OC) was investigated in human periodontal ligament cells, by an MTT assay, qPCR and western blot analysis. The results demonstrated that icariin promoted cell proliferation in a dose- and time-dependent manner, upregulated OPG, Cbfa1 and OC expression, and downregulated RANKL production and the RANKL/OPG expression ratio. This suggested the potential value of icariin in treating alveolar bone resorption and promoting periodontal tissue regeneration, due to its ability to stimulate the proliferation and osteogenic differentiation of human periodontal ligament cells and inhibit osteoclast differentiation.
Department of Periodontics, Capital Medical University, School of Stomatology, Beijing 100050, P.R. China.
Zhenhua
Z
Pei
Fengqiu
F
Zhang
Zhongying
Z
Niu
Shenggen
S
Shi
D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000378:metabolism*; D015153:Blotting, Western; D001862:Bone Resorption / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D053244:Osteoprotegerin / Q000235:genetics / Q000378:metabolism; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D053245:RANK Ligand / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
D015415:Biomarkers; D050659:Core Binding Factor Alpha 1 Subunit; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger; D015675:Osteocalcin; C056599:icariin