[Study on human aFGF fusion gene transformation with soybean 24 kDa oleosin and expression in safflower].
OBJECTIVE
To investigate the expression of acidic fibroblast growth factor (aFGF) in transgenic safflower and lay the foundation for the use of the plant bioreactor large-scale production aFGF.


METHODS
The haFGF gene was transformed into plant preference of the aFGF sequence as a basis for design of primers, plant preferences aFGF gene sequences was amplified by PCR. The vegetable body expression vector was constructed by using digested connection method and then transferred to Agrobacterium tumefaciens EHA105 by the freeze-thaw method. It transferred to safflowers by agrobacterium-mediated transformation method, and identified by PCR, southern blot and RT-PCR.


RESULTS
The full-length aFGF gene sequences were amplified through PCR and constructed into plant expression vector with soybean oleosin and promoter, and transformed into safflower. Three independently transformed safflower plant units with point insertion were successfully obtained, which showed the same size of aFGF expression at the transcriptional level.


CONCLUSIONS
The plant oil body expression vectors were successfully constructed, and the optimal condition for genetic transformation was selected. The transgenic safflower plants were obtained.
Agricultural Sciences College, Jilin Agricultural University, Changchun 130118, China. 148050459@qq.com
Yu-Hong
YH
Gai
Wang
W
Wang
Xiao
X
Jin
Jia-Qi
JQ
Wang
Hong-Bo
HB
Li
Gang
G
Wang
Jing
J
Yang
Jian
J
Wei
D016960:Agrobacterium tumefaciens / Q000235:genetics; D031187:Carthamus tinctorius / Q000235:genetics*; D016220:Fibroblast Growth Factor 1 / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic
D008565:Membrane Proteins; D010940:Plant Proteins; C079299:oleosin 24 protein, Glycine max; D016220:Fibroblast Growth Factor 1