Tuning molecular interactions in lipid-oligonucleotides assemblies via locked nucleic acid (LNA)-based lipids.
Hybrid nucleotide-lipids containing locked nucleic acid (LNA) show enhanced hybridization properties with complementary single strand RNAs compared to DNA lipid analogues. The LNA adenosine lipid features unique binding properties with a high binding affinity for poly-uridine and the entropically driven formation of a stable complex (K(d) ≈ 43 nM). Enhanced hybridization properties of LNA-based lipids should be applicable for the development of oligonucleotide (ON) delivery systems or as small molecule binders to RNA for novel therapeutic strategies.
Univ. Bordeaux, ARNA laboratory, F-33076 Bordeaux, France. philippe.barthelemy@inserm.fr.
Amit
A
Patwa
Gilmar
G
Salgado
François
F
Dole
Laurence
L
Navailles
Philippe
P
Barthélémy
D008055:Lipids / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*
D008055:Lipids; D009841:Oligonucleotides; C477371:locked nucleic acid