Adsorption of a PEO-PPO-PEO triblock copolymer on metal oxide surfaces with a view to reducing protein adsorption and further biofouling.
Abstract Biomolecule adsorption is the first stage of biofouling. The aim of this work was to reduce the adsorption of proteins on stainless steel (SS) and titanium surfaces by modifying them with a poly(ethylene oxide) (PEO)-poly(propylene oxide) (PPO)-PEO triblock copolymer. Anchoring of the central PPO block of the copolymer is known to be favoured by hydrophobic interaction with the substratum. Therefore, the surfaces of metal oxides were first modified by self-assembly of octadecylphosphonic acid. PEO-PPO-PEO preadsorbed on the hydrophobized surfaces of titanium or SS was shown to prevent the adsorption of bovine serum albumin (BSA), fibrinogen and cytochrome C, as monitored by quartz crystal microbalance (QCM). Moreover, X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry were used to characterize the surfaces of the SS and titanium after competitive adsorption of PEO-PPO-PEO and BSA. The results show that the adsorption of BSA is well prevented on hydrophobized surfaces, in contrast to the surfaces of native metal oxides.
a Surfaces Group (SURF), Bio- and Soft Matter (BSMA) , Institute of Condensed Matter and Nanosciences (IMCN), Université catholique de Louvain , Louvain-la-Neuve , Belgium.
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Y
Yang
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Poleunis
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Románszki
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J
Telegdi
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Dupont-Gillain
D000327:Adsorption; D000818:Animals; D057219:Biofouling / Q000517:prevention & control*; D002417:Cattle; D045304:Cytochromes c / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism*; D006736:Horses; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D013058:Mass Spectrometry; D010757:Phosphorous Acids / Q000737:chemistry; D056951:Photoelectron Spectroscopy; D011092:Polyethylene Glycols / Q000737:chemistry*; D011409:Propylene Glycols / Q000737:chemistry*; D058525:Quartz Crystal Microbalance Techniques; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013193:Stainless Steel / Q000737:chemistry; D014025:Titanium / Q000737:chemistry
C116176:PEO-PPO-PEO; D010757:Phosphorous Acids; D011409:Propylene Glycols; D013193:Stainless Steel; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D005340:Fibrinogen; D045304:Cytochromes c; D014025:Titanium