A photo-triggered layered surface coating producing reactive oxygen species.
We report a photoactive surface coating which produces cytotoxic reactive oxygen species (ROS) upon irradiation with near infrared (NIR) light. The coating is assembled layer-by-layer, and consists of cross-linked hyaluronic acid (HA) and poly-l-lysine (PLL) modified with the photoactive molecule pheophorbide a. Pheophorbide a loading can be fine-tuned by varying the number of bilayers, yielding stable materials with the capacity to generate repeated and/or prolonged light-triggered ROS release. Light irradiation of the photoactive surface coatings provides a versatile platform for the spatiotemporal control of events at the material-tissue interface, such as bacterial colonization, platelet adhesion, and mammalian cell attachment.
Laboratory for Biomaterials and Drug Delivery, Department of Anesthesiology, Division of Critical Care Medicine, Children's Hospital Boston, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA; Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Doris
D
Gabriel
Isa P
IP
Monteiro
David
D
Huang
Robert
R
Langer
Daniel S
DS
Kohane
D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D008027:Light; D010777:Photochemistry; D053657:Platelet-Rich Plasma / Q000528:radiation effects; D011107:Polylysine / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000737:chemistry / Q000378:metabolism; D013211:Staphylococcus aureus / Q000378:metabolism
D017382:Reactive Oxygen Species; D011107:Polylysine; D006820:Hyaluronic Acid
Coating; Layer by layer; Photo-triggered; Reactive oxygen species