Arabidopsis C-terminal domain phosphatase-like 1 functions in miRNA accumulation and DNA methylation.
Arabidopsis CTD-PHOSPHATASE-LIKE 1 (CPL1) is a protein phosphatase that can dephosphorylate RNA polymerase II C-terminal domain (CTD). Unlike typical CTD-phosphatases, CPL1 contains a double-stranded (ds) RNA-binding motif (dsRBM) and has been implicated for gene regulation mediated by dsRNA-dependent pathways. We investigated the role of CPL1 and its dsRBMs in various gene silencing pathways. Genetic interaction analyses revealed that cpl1 was able to partially suppress transcriptional gene silencing and DNA hypermethylation phenotype of ros1 suggesting CPL1 is involved in the RNA-directed DNA methylation pathway without reducing siRNA production. By contrast, cpl1 reduced some miRNA levels at the level of processing. Indeed, CPL1 protein interacted with proteins important for miRNA biogenesis, suggesting that CPL1 regulates miRNA processing. These results suggest that CPL1 regulates DNA methylation via a miRNA-dependent pathway.
Division of Applied Life Science (BK21 Program), Graduate School of Gyeongsang National University, Jinju, Gyeongsangnam-do, Korea ; Department of Horticultural Sciences, Texas A&M University, College Station, Texas, United States of America.
In Sil
IS
Jeong
Emre
E
Aksoy
Akihito
A
Fukudome
Salina
S
Akhter
Akihiro
A
Hiraguri
Toshiyuki
T
Fukuhara
Jeong Dong
JD
Bahk
Hisashi
H
Koiwa
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003080:Cold Temperature; D019175:DNA Methylation / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D056426:Genetic Loci / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological / Q000235:genetics; D013489:Suppression, Genetic; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D029681:Arabidopsis Proteins; D035683:MicroRNAs; D018749:RNA, Plant; D016601:RNA-Binding Proteins; D014157:Transcription Factors; C465438:CPL1 protein, Arabidopsis; D010749:Phosphoprotein Phosphatases