Simultaneous display of two large proteins on the head and tail of bacteriophage lambda.
BACKGROUND
Consistent progress in the development of bacteriophage lambda display platform as an alternative to filamentous phage display system was achieved in the recent years. The lambda phage has been engineered to display efficiently multiple copies of peptides or even large protein domains providing a powerful tool for screening libraries of peptides, proteins and cDNA.


RESULTS
In the present work we describe an original method for dual display of large proteins on the surface of lambda particles. An anti-CEA single-chain antibody fragment and green fluorescent protein or alkaline phosphatase were simultaneously displayed by engineering both gpD and gpV lambda proteins.


CONCLUSIONS
Here we show that such modified phage particles can be used for the detection of target molecules in vitro and in vivo. Dual expression of functional moieties on the surface of the lambda phage might open the way to generation of a new class of diagnostic and therapeutic targeted nanoparticles.
Biotechnology, Sigma-tau, SpA, Pomezia (RM) 00040, Italy. olga.minenkova@sigma-tau.it.
Emiliano
E
Pavoni
Paola
P
Vaccaro
Valeria
V
D'Alessio
Rita
R
De Santis
Olga
O
Minenkova
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics*; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D019073:HT29 Cells; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry; D019151:Peptide Library; D010957:Plasmids / Q000235:genetics; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D036022:Capsid Proteins; D018076:DNA, Complementary; D006023:Glycoproteins; D019151:Peptide Library; D011506:Proteins; D011993:Recombinant Fusion Proteins; C406073:gpD protein, lambda phage; D049452:Green Fluorescent Proteins; D000469:Alkaline Phosphatase