Inducible control of gene expression with destabilized Cre.
Acute manipulation of gene and protein function in the brain is essential for understanding the mechanisms of nervous system development, plasticity and information processing. Here we describe a technique based on a destabilized Cre recombinase (DD-Cre) whose activity is controlled by the antibiotic trimethoprim (TMP). We show that DD-Cre triggers rapid TMP-dependent recombination of loxP-flanked ('floxed') alleles in mouse neurons in vivo and validate the use of this system for neurobehavioral research.
1] Department of Molecular and Cellular Neuroscience, The Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, California, USA. [2] The Kellogg School of Science and Technology, The Scripps Research Institute, La Jolla, California, USA.
Richard
R
Sando
Karsten
K
Baumgaertel
Simon
S
Pieraut
Nina
N
Torabi-Rander
Thomas J
TJ
Wandless
Mark
M
Mayford
Anton
A
Maximov
D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D019426:Integrases / Q000378:metabolism*; D051379:Mice; D011995:Recombination, Genetic / Q000187:drug effects; D014295:Trimethoprim / Q000494:pharmacology
D014295:Trimethoprim; C045073:Cre recombinase; D019426:Integrases