In vitro trypanocidal activity of solamargine and extracts from Solanum palinacanthum and Solanum lycocarpum of Brazilian cerrado.
The present investigation was to evaluate the potential trypanocidal activity of crude ethanolic extract of the fruits of Solanum palinacanthum, Solanum lycocarpum and the glycoalcaloid, solamargine. S. palinacanthum and S. lycocarpum fruit powders were submitted to exhaustively extraction with 96% ethanol and solamargine were isolated from the extract of S. palinacanthum. Both extracts and solamargine were analysed for trypanocidal activity by using MTT colorimetric assay. Extracts of S. palinacanthum showed to be more active (IC50 = 175.9 µg.ml-1) than S. lycocarpum (IC50 = 194.7 µg.ml-1). Solamargine presented a strong activity (IC50 = 15.3 µg.ml-1), which can explain the better activity of the both extracts. Benznidazol (IC50 = 9.0 µg.ml-1) is the only drug used to treat Chagas' disease. These findings demonstrate for the first time that ethanol extracts obtained from both fruits of S. palinacanthum and S. lycocarpum and also solamargine have a potential anti-trypanosomal activity.
Departamento de Princípios Ativos Naturais e Toxicologia, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista/UNESP, Rodovia Araraquara – Jaú, Km 1, s/n, 14801-502 Araraquara, SP, Brasil. moreirar@fcfar.unesp.br
Raquel R D
RR
Moreira
Gilmarcio Z
GZ
Martins
Nathália O
NO
Magalhães
Adélia E
AE
Almeida
Rosemeire C L R
RC
Pietro
Flávia A J
FA
Silva
Regina M B
RM
Cicarelli
D020128:Inhibitory Concentration 50; D021261:Parasitic Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D012991:Solanaceous Alkaloids / Q000494:pharmacology*; D032322:Solanum / Q000737:chemistry* / Q000145:classification; D014344:Trypanocidal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects*
D010936:Plant Extracts; D012991:Solanaceous Alkaloids; D014344:Trypanocidal Agents; C026608:alpha-solamargine