Immunomodulatory activities of polysaccharides isolated from Taxillus chinensis and Uncaria rhyncophylla.
Taxillus chinensis and Uncaria rhyncophylla are the herbs used in traditional Chinese anticancer formulations. During the past decade, research on plant polysaccharides has gained importance due to their therapeutic value and minimum side effects. In this study, hot water extraction method was employed to isolate polysaccharides from the stems of T. chinensis and stems with hooks of U. rhyncophylla. Size-exclusion chromatography was then used for further fractionation. Separated fractions from T. chinensis were designated as TCP-1, TCP-2 and TCP-3 and those from U. rhyncophylla were termed UC-1 and UC-2. Their sugar compositions were estimated using gas chromatography that revealed the presence fructose, glucose, xylose, arbinose, and rhamnose. Amino acid analysis of these fractions has indicated that they are protein-bound polysaccharides. The antioxidant activities were investigated using DPPH and yeast assays. The ability of these polysaccharide fractions to stimulate mouse macrophages was measured using Griess reagent and ELISA test. The results revealed that some of the isolated fractions (TCP-2, TCP-3, UC-1 and UC-2) displayed significant antioxidant activities and were also found to be effective immunomodulators in a concentration-dependent manner. Outcomes of this research strongly indicate that U. rhyncophylla and T. chinensis have therapeutic potential to be used for the treatment of cancer.
School of Science and Health, University of Western Sydney, Locked Bag 1797, Penrith South DC, NSW 1797, Australia.
Lin
L
Zhang
Sundar Rao
SR
Koyyalamudi
Sang Chul
SC
Jeong
Narsimha
N
Reddy
Trevor
T
Bailey
T
T
Longvah
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002460:Cell Line; D045744:Cell Line, Tumor; D002850:Chromatography, Gel; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006358:Hot Temperature; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D028183:Loranthaceae / Q000737:chemistry*; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D051379:Mice; D009005:Monosaccharides / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D012997:Solvents; D032070:Uncaria / Q000737:chemistry*; D014867:Water
D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D007155:Immunologic Factors; D009005:Monosaccharides; D010936:Plant Extracts; D011134:Polysaccharides; D012997:Solvents; D014867:Water
Antioxidant activities; Immunomodulatory effects; Polysaccharides; T. chinensis; U. rhyncophylla