Rescuing recombinant proteins by sequestration into the P22 VLP.
Here we report the use of a self-assembling protein cage to sequester and solubilize recombinant proteins which are usually trafficked to insoluble inclusion bodies. Our results suggest that protein cages can be used as novel vehicles to rescue and produce soluble proteins that are otherwise difficult to obtain using conventional methods.
Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, USA.
Dustin P
DP
Patterson
Benjamin
B
LaFrance
Trevor
T
Douglas
D017100:Bacteriophage P22 / Q000737:chemistry*; D002213:Capsid / Q000737:chemistry; D005818:Genetic Engineering / Q000379:methods*; D002479:Inclusion Bodies / Q000378:metabolism; D008958:Models, Molecular; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics*; D012995:Solubility
D011994:Recombinant Proteins