FFAs and adipokine-mediated regulation of hsa-miR-143 expression in human adipocytes.
Accumulating evidence suggests that microRNAs (miRNAs) play an important role in regulating the pathways in adipose tissue that control processes such as adipogenesis, insulin resistance, and inflammation. MiR-143 is a well-characterized miRNA involved in adipogenesis and may be involved in regulating insulin resistance. Free fatty acids (FFAs) and adipokines, such as tumor necrosis factor-α (TNF-α), leptin, resistin, and interleukin-6 (IL-6), have already been identified as main regulators of obesity and insulin sensitivity. Therefore, we studied the effects of these inflammatory cytokines on the expression of miR-143. FFAs, resistin, and leptin downregulated miR-143 expression in human adipocytes, whereas TNF-α and IL-6 had little effect on miR-143 expression. These results suggest that the expression of miR-143 is affected by a variety of factors that are related to insulin sensitivity. Therefore, miR-143 may be an important mediator in the development of obesity-related insulin resistance.
Department of Endocrinology, Second Affiliated Hospital, Nanjing Medical University, 210011, Nanjing, China.
Lingling
L
Zhu
Chunmei
C
Shi
Chenbo
C
Ji
Guangfeng
G
Xu
Ling
L
Chen
Lei
L
Yang
Ziyi
Z
Fu
Xianwei
X
Cui
Yibing
Y
Lu
Xirong
X
Guo
D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D054392:Adipokines / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology; D020738:Leptin / Q000494:pharmacology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D052243:Resistin / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D054392:Adipokines; D005230:Fatty Acids, Nonesterified; D015850:Interleukin-6; D020738:Leptin; C542359:MIRN143 microRNA, human; D035683:MicroRNAs; D052243:Resistin; D014409:Tumor Necrosis Factor-alpha