Activity of ceftaroline against Enterococcus faecium PBP5.
The opportunistic human pathogen Enterococcus faecium overproduces the low-affinity PBP5. In clinical strains, mutations in PBP5 further reduce its acylation rate by β-lactams. Previous studies have reported that ceftaroline had poor inhibitory activity against β-lactam-resistant E. faecium strains. In this study, we show that ceftaroline exhibits killing activity against our laboratory-derived ampicillin-resistant E. faecium mutant that overproduces a wild-type PBP5 and that ceftaroline inactivates PBP5 much faster than benzylpenicillin and faster than ceftobiprole.
Bacterial Physiology and Genetics Unit, Center for Protein Engineering, Life Science Department, University of Liège, Liège, Belgium.
Xavier
X
Henry
Oliver
O
Verlaine
Ana
A
Amoroso
Jacques
J
Coyette
Jean-Marie
JM
Frère
Bernard
B
Joris
D000668:Ampicillin Resistance / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D002511:Cephalosporins / Q000494:pharmacology*; D016984:Enterococcus faecium / Q000187:drug effects* / Q000235:genetics*; D018440:beta-Lactam Resistance / Q000235:genetics; D000097583:Ceftaroline
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002511:Cephalosporins; D000097583:Ceftaroline