In vitro action of combinations of antimicrobial agents with EDTA-tromethamine on Proteus vulgaris of a canine origin.
Combinations of EDTA-tromethamine and 7 antimicrobial agents (chloramphenicol, nalidixic acid, oxytetracycline, penicillin, polymyxin-B, streptomycin, or a combination of sulfamethazine, sulfapyridine, and sulfathiazole) were tested for synergistic activities against Proteus vulgaris of canine origin. Three in vitro tests were used, including minimal inhibitory concentrations of the drugs, a 2-dimensional microtiter checkerboard technique, and bacterial inhibition studies. A synergistic inhibitory action was observed with combinations of EDTA-tromethamine plus penicillin, oxytetracycline, nalidixic acid, chloramphenicol, polymyxin-B, or sulfamethazine-sulfapyridine-sulfathiazole by at least one test system. A synergistic effect was not recorded with combinations of EDTA-tromethamine and streptomycin. Poor correlation between data from the 3 test systems was noticed, despite the fact that each monitors bacteriostatic end points.
Department of Medical Microbiology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
R E
RE
Wooley
M S
MS
Jones
J P
JP
Gilbert
E B
EB
Shotts
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D004283:Dog Diseases / Q000382:microbiology; D004285:Dogs; D004492:Edetic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D010032:Otitis Externa / Q000382:microbiology / Q000662:veterinary*; D011512:Proteus Infections / Q000382:microbiology / Q000662:veterinary*; D011514:Proteus vulgaris / Q000187:drug effects*; D014325:Tromethamine / Q000031:analogs & derivatives* / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D014325:Tromethamine; D004492:Edetic Acid