Homoarginine, β-ODAP, and asparagine contents of grass pea landraces cultivated in Turkey.
The seeds of grass pea (Lathyrus sativus L.), a drought tolerant crop, were analysed for quantitative determination of the free amino acids β-N-oxalyl-l-α,β-diaminopropionic acid (β-ODAP), homoarginine and asparagine by a simple and fast capillary electrophoretic method. In boric acid (80mM, pH 8.0) running buffer system, not only were α and β-ODAP successfully separated, but also an efficient sample stacking was achieved during hydrodynamic sample introduction. The validated method was used for quantification of β-ODAP, homoarginine and asparagine in seed extracts of 52 Lathyrus local landraces from various regions of Turkey and one released cultivar. The concentration ranges of amino acids were found as 0.21-1.27% (w/w) for homoarginine, 0.10-0.87% (w/w) for β-ODAP and 0.006-0.47% (w/w) for asparagine. A positive correlation between homoarginine and β-ODAP quantities in seeds of 53 Lathyrus local landraces was shown to exist (r(2)=0.649).
Science and Art Faculty, Department of Chemistry, Ondokuz Mayıs University, Kurupelit, Samsun 55139, Turkey. Electronic address: nonar@omu.edu.tr.
A Nur
AN
Onar
Behice Yavuz
BY
Erdoğan
Ilknur
I
Ayan
Zeki
Z
Acar
D000599:Amino Acids, Diamino / Q000032:analysis*; D001216:Asparagine / Q000032:analysis*; D006709:Homoarginine / Q000032:analysis*; D027807:Lathyrus / Q000737:chemistry* / Q000254:growth & development; D010936:Plant Extracts / Q000032:analysis*; D012639:Seeds / Q000737:chemistry; D014421:Turkey
D000599:Amino Acids, Diamino; D010936:Plant Extracts; D006709:Homoarginine; C100126:oxalyldiaminopropionic acid; D001216:Asparagine
Asparagine; Capillary electrophoresis; Homoarginine; Lathyrus sativus L.; β-ODAP