Comprehensive screening for multi-class veterinary drug residues and other contaminants in muscle using column-switching UPLC-MS/MS.
A quantitative multi-class analytical method covering more than 226 veterinary drugs and other contaminants in muscle, belonging to different drug families, was developed. The method is based on liquid-liquid extraction, purification by low-temperature clean-up and dispersive solid-phase extraction (D-SPE), and analysis was conducted in two analytical runs by column-switching UPLC coupled with electrospray ionisation and tandem mass spectrometry (UPLC-ESI-MS/MS). For most of the target analytes, the optimised pre-treatment processes led to no significant interference from the sample matrix. The limit of quantification varied from 0.05 to 10 µg kg(-1). Statistical evaluation indicated that average recoveries spiked into pork were in the range from 62.4% to 138.8%, and the relative standard deviations were in the range from 2.8% to 26.6% (intra-day precision). The availability of this method will contribute to a better safety assurance of meat with a significant reduction of both effort and time.
a Ningbo Academy of Inspection and Quarantine of the P.R. China , Ningbo , P.R. China.
Jia
J
Zhan
Di-ming
DM
Xu
Shi-jie
SJ
Wang
Ji
J
Sun
Yin-jun
YJ
Xu
Mei-lin
ML
Ni
Ju-yi
JY
Yin
Jie
J
Chen
Xue-jun
XJ
Yu
Zhi-qiang
ZQ
Huang
D000818:Animals; D002417:Cattle; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry; D015203:Reproducibility of Results; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*; D019155:Veterinary Drugs / Q000032:analysis*
D019155:Veterinary Drugs