Self-supported fibrin-polyvinyl alcohol interpenetrating polymer networks: an easily handled and rehydratable biomaterial.
A fibrin hydrogel at physiological concentration (5 mg/mL) was associated with polyvinyl alcohol (PVA) inside an interpenetrating polymer networks (IPN) architecture. Previously, PVA has been modified with methacrylate functions in order to cross-link it by free-radical polymerization. The fibrin network was synthesized by the enzymatic hydrolysis of fibrinogen by thrombin. The resulting self-supported materials simultaneously exhibit the properties of the fibrin hydrogel and those of the synthetic polymer network. Their storage modulus is 50-fold higher than that of the fibrin hydrogel and they are completely rehydratable. These materials are noncytotoxic toward human fibroblast and the fibrin present on the surface of PVAm-based IPNs favors cell development.
Equipe de Recherche sur les Relations Matrice Extracellulaire Cellules (Errmece), ‡Laboratoire de Physicochimie des Polymères et des Interfaces (LPPI), Institut des Matériaux, University of Cergy-Pontoise , 95000 Cergy-Pontoise, France.
Laurent
L
Bidault
Marie
M
Deneufchatel
Cédric
C
Vancaeyzeele
Odile
O
Fichet
Véronique
V
Larreta-Garde
D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005337:Fibrin / Q000737:chemistry* / Q000378:metabolism; D005340:Fibrinogen / Q000737:chemistry / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D052816:Foreskin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D008297:Male; D010316:Particle Size; D011142:Polyvinyl Alcohol / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013499:Surface Properties; D013917:Thrombin / Q000378:metabolism; D014867:Water / Q000737:chemistry / Q000378:metabolism
D001672:Biocompatible Materials; D014867:Water; D005337:Fibrin; D005340:Fibrinogen; D011142:Polyvinyl Alcohol; D013917:Thrombin