Direct inhibition of cell surface ephrin-B2 by recombinant ephrin-B2/FC.
First messengers and viral transfection are the two most common ways to stimulate cells for signal output, although their applications are limited. We investigated mechanisms of inducing neural stem cell differentiation using recombinant ephrin-B2/Fc and found that it acted as a ligand and inhibited endogenous ephrin-B2, which maintenance of the neural progenitor cell state, by direct interference. Our results showed the movement of ephrin-B2/Fc within the cell and indicated that it recycled to the plasma membrane surface, revealing a possible pattern of ephrin trafficking. Our results also serve as proof of concept for the reconstruction of the intracellular domain of ephrin using an artificial receptor to direct input signals in future studies.
Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu Province 226001, China.
Hu
H
Xiaodong
Huang
H
Zhen
Sun
S
Min
Cui
C
Zhiming
Ji
J
Hongyan
Zhang
Z
Chong
Tan
T
Xuefeng
Jin
J
Guohua
D000818:Animals; D002478:Cells, Cultured; D004705:Endocytosis / Q000187:drug effects; D036388:Ephrin-B2 / Q000276:immunology / Q000502:physiology*; D007141:Immunoglobulin Fc Fragments / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction / Q000502:physiology*; D013234:Stem Cells / Q000502:physiology*
D036388:Ephrin-B2; D007141:Immunoglobulin Fc Fragments
Endocytosis; Ephrin-B2; Neural stem cell; Neurogenesis; Signal transduction