Combined antihypertensive effect of luteolin and buddleoside enriched extracts in spontaneously hypertensive rats.
BACKGROUND
Flos Chrysanthemi is used in a variety of diseases in traditional Chinese medicine including hypertension, and the total flavonoids (rich in luteolin (LUT) and buddleoside (BUD)) of Flos Chrysanthemi is known to modulate vascular functions and reduce the blood pressure. However, the active flavonoids and their synergistic effects on anti-hypertension are still unclear. To investigate the combined anti-hypertension effects of LUT and BUD enriched extracts on spontaneously hypertensive rats (SHR), as well as the anti-hypertensive mechanism of LUT&BUD mixture.


METHODS
CODA Mouse & Rat Tail-Cuff Blood Pressure System was used to measure the systolic blood pressure (SBP) and diastolic blood pressure (DBP) of SHR after treated with extracts contains with LUT and/or BUD. The expressions of Ang II, PRA, ALD, ET, PGI2 and TXB2 were investigated by ELASA. Serum NO concentration was measured by the method of Nitric acid reductase.


RESULTS
A single administration of LUT, BUD, or LUT:BUD=1:1 significantly reduced SBP by about 3.35 mmHg, 4.39 mmHg and 15.42 mmHg, respectively. Chronic administration of LBM (at 60 mg/kg; p.o. for 30 days) reduced both SBP and DBP by 4.04% and 5.24% of the vehicle group, respectively. Oral administration of LBM at 60 mg/kg inhibited the serum levels of ANG, ALD and ET, but increased serum NO concentration.


CONCLUSIONS
This study shows the synergistic anti-hypertension effects of LUT and BUD in SHR. The effects of LBM on blood pressure are associated with RAAS and endothelial system. Thus, our experiments suggest that the combination of luteolin and buddleoside from Flos Chrysanthemi are potentially useful for the therapeutic treatments for hypertension.
Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, PR China; Wenzhou Medical University, Wenzhou, Zhejiang 325035, PR China. Electronic address: lv.gy@263.net.
Gui-Yuan
GY
Lv
Yan-Ping
YP
Zhang
Jian-Li
JL
Gao
Jing-Jing
JJ
Yu
Jing
J
Lei
Zhi-Ru
ZR
Zhang
Bo
B
Li
Ruan-Juan
RJ
Zhan
Su-Hong
SH
Chen
D000450:Aldosterone / Q000097:blood; D000804:Angiotensin II / Q000097:blood; D000818:Animals; D000959:Antihypertensive Agents / Q000627:therapeutic use*; D004359:Drug Therapy, Combination; D016232:Endothelins / Q000097:blood; D011464:Epoprostenol / Q000097:blood; D006027:Glycosides / Q000627:therapeutic use*; D006973:Hypertension / Q000097:blood / Q000188:drug therapy*; D047311:Luteolin / Q000627:therapeutic use*; D008297:Male; D009569:Nitric Oxide / Q000097:blood; D051381:Rats; D011918:Rats, Inbred SHR; D012083:Renin / Q000097:blood; D013929:Thromboxane B2 / Q000097:blood
D000959:Antihypertensive Agents; D016232:Endothelins; D006027:Glycosides; D000804:Angiotensin II; D009569:Nitric Oxide; D000450:Aldosterone; D013929:Thromboxane B2; D011464:Epoprostenol; D012083:Renin; C008282:linarin; D047311:Luteolin
Buddleoside; Flos Chrysanthemi; Hypertension; Luteolin; Synergy