Decreased level of intracellular cholesterol in peripheral blood mononuclear cells is associated with chronic hepatitis C virus infection.
Peripheral blood mononuclear cells (PBMCs) constitute the main extrahepatic place of, hepatitis C virus (HCV) replication. We aimed to determine the impact of CHC infection and microRNA-, 122 expression on cholesterol expression in PBMCs. HCV RNA strand, intracellular cholesterol, HMGCoA, reductase and miR-122 expression in PBMC were determined in 54 CHC patients. The study shows that significant decrease of intracellular cholesterol level in PBMC (p=0.000000), accompanied by serum hypocholesterolemia is the characteristic feature of chronic hepatitis C infection. Although, microRNA-122 expression was detectable in PBMCs of CHC patients (52.5%), the alteration of intracellular cholesterol level was independent of miR-122 expression.
Department of Medical Biochemistry, Medical University of Lodz, Mazowiecka 6/8, 92-215 Lodz, Poland. Electronic address: malgorzata.sidorkiewicz@umed.lodz.pl.
Malgorzata
M
Sidorkiewicz
Martyna
M
Grek
Barbara
B
Jozwiak
Anna
A
Piekarska
D000328:Adult; D002784:Cholesterol / Q000032:analysis* / Q000097:blood; D005260:Female; D016174:Hepacivirus / Q000235:genetics; D019698:Hepatitis C, Chronic / Q000473:pathology*; D006801:Humans; D006903:Hydroxymethylglutaryl CoA Reductases / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D055815:Young Adult
C531907:MIRN122 microRNA, human; D035683:MicroRNAs; D012367:RNA, Viral; D002784:Cholesterol; D006903:Hydroxymethylglutaryl CoA Reductases
Cholesterol; Chronic hepatitis C infection; Mir-122; PBMCs