Interleukin-6 and tumor necrosis factor-alpha gene polymorphisms in chronic idiopathic urticaria.
BACKGROUND
This study was performed to evaluate association of gene polymorphisms among proinflammatory cytokines and susceptibility to chronic idiopathic urticaria (CIU).


METHODS
Ninety patients with prolonged urticaria more than 6 weeks were included as case group. Single nucleotide polymorphisms (SNPs) of IL-6 (G/C -174, G/A nt565) and TNF-α (G/A -308, G/A -238) were evaluated, using polymerase chain reaction (PCR); and the results were compared to the control group.


RESULTS
G allele was significantly higher in the patients at locus of -238 of promoter of TNF-α gene (p<0.001). Frequency of following genotypes were significantly lower in patients with CIU, compared to controls: AG at -308 and GA at -238 of TNF-α gene (p<0.05 and p<0.001, respectively), CG at -174 and GG at +565 of IL-6 gene (p<0.05). Additionally, following genotypes were more common among patients with CIU: GG at -308 and -238 of TNF-α gene (p<0.05 and p<0.001, respectively), GG at -174 and GA at +565 of IL-6 gene (p<0.05).


CONCLUSIONS
Pro-inflammatory cytokine gene polymorphisms can affect susceptibility to CIU. TNF-α promoter polymorphisms as well as IL-6 gene polymorphisms are associated with CIU.
Department of Pediatrics, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
M
M
Tavakol
Molecular Immunology Research Center, and Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
A A
AA
Amirzargar
Department of Pediatrics, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran; Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: movahedm@tums.ac.ir.
M
M
Movahedi
Research Center for Immunodeficiencies, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
Z
Z
Aryan
Molecular Immunology Research Center, and Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
A Z
AZ
Bidoki
Department of Pediatrics, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
M
M
Gharagozlou
Department of Pediatrics, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran; Research Center for Immunodeficiencies, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
A
A
Aghamohammadi
Department of Allergy and Immunology, Rasool-e-Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran.
M
M
Nabavi
Research Center for Rational Use of Drugs, Tehran University of Medical Sciences, Tehran, Iran.
A
A
Ahmadvand
Department of Pediatrics, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
N
N
Behniafard
Department of Pediatrics, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran; Molecular Immunology Research Center, and Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
K
K
Heidari
Department of Pediatrics, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran; Molecular Immunology Research Center, and Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
S
S
Soltani
Molecular Immunology Research Center, and Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Research Center for Immunodeficiencies, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
N
N
Rezaei
D002908:Chronic Disease; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D007492:Iran; D008297:Male; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D014581:Urticaria / Q000235:genetics / Q000276:immunology*
D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha
Interleukin 6; Single nucleotide polymorphism; Tumor necrosis factor alpha; Urticaria