Impact of iron oxide nanoparticles on brain, heart, lung, liver and kidneys mitochondrial respiratory chain complexes activities and coupling.
The present study evaluates the effects of iron oxide nanoparticles (ION) on mitochondrial respiratory chain complexes activities in five organs characterized by different oxidative capacities and strongly involved in body detoxification. Isolated mitochondria were extracted from brain, heart, lung, liver and kidneys in twelve Wistar rats (8 weeks) using differential centrifugations. Maximal oxidative capacities (Vmax), mitochondrial respiratory chain complexes activity using succinate (Vsucc, complexes II, III, and IV activities) or N, N, N', N'-tetramethyl-p-phenylenediaminedihydrochloride (tmpd)/ascorbate (Vtmpd, complex IV activity) and, mitochondrial coupling (Vmax/Vo) were determined in controls and after exposure to 100, 200, 300 and 500μg/ml Fe3O4. Data showed that baseline maximal oxidative capacities were 26.3±4.7, 48.9±4.6, 11.3±1.3, 27.0±2.5 and 13.4±1.7μmol O2/min/g protein in brain, heart, lung, liver, and kidneys mitochondria, respectively. Complexes II, III, and IV activities also significantly differed between the five organs. Interestingly, as compared to baseline values and in all tissues examined, exposure to ION did not alter mitochondrial respiratory chain complexes activities whatever the nanoparticles (NPs) concentration used. Thus, ION did not show any toxicity on mitochondrial coupling and respiratory chain complexes I, II, III, and IV activities in these five major organs.
Université de Strasbourg, Fédération de Médecine Translationnelle, EA 3072: Mitochondries, Stress oxydant et Protection musculaire, Faculté de Médecine, 67000 Strasbourg, France; Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, Université de Carthage, 7021 Jarzouna, Tunisia.
Yosra
Y
Baratli
Anne-Laure
AL
Charles
Valérie
V
Wolff
Lotfi
L
Ben Tahar
Leila
L
Smiri
Jamal
J
Bouitbir
Joffrey
J
Zoll
François
F
Piquard
Olfa
O
Tebourbi
Mohsen
M
Sakly
Hafedh
H
Abdelmelek
Bernard
B
Geny
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism*; D006321:Heart / Q000187:drug effects / Q000502:physiology; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000633:toxicity*; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D045222:Electron Transport Chain Complex Proteins; D058185:Magnetite Nanoparticles
ACR; Coupling; DEG; ION; Ironoxide; Mitochondria; Mitochondrial respiratorychain; NPs; Nanoparticles; Vmax; Vsucc; Vtmpd; acceptor control ratio; complex IV activity; complexes II, III and IV activities; diethyleneglycol; iron oxide nanoparticles; maximal oxidative capacities; nanoparticles