Dynamic and reversible fluorescence imaging of superoxide anion fluctuations in live cells and in vivo.
Overgeneration of reactive oxygen species (ROS) is closely associated with cellular damage and diseases. As superoxide anion (O2(•-)) is the precursor of other ROS, exploring O2(•-) fluctuations in cells and in vivo is of great significance. To address this critical need, we have developed a novel reversible fluorescent probe with one-photon and two-photon fluorescence properties, which is well suited for monitoring O2(•-) fluxes selectively and dynamically. Imaging results substantiate dynamic and reversible fluorescence responses of this probe to intracellular O2(•-) under apoptotic stimuli. Moreover, this probe can conveniently visualize changes in O2(•-) concentration during reperfusion injury in hepatocytes, zebrafish, and mice, by means of one-photon or two-photon imaging according to depths of various samples. The present study provides a powerful fluorescent imaging tool for dynamic tracking of O2(•-) in live cells and in vivo.
College of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Ministry of Education, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University , Jinan 250014, P.R. China.
Wen
W
Zhang
Ping
P
Li
Fan
F
Yang
Xiufen
X
Hu
Chuanzhi
C
Sun
Wei
W
Zhang
Dezhan
D
Chen
Bo
B
Tang
D000818:Animals; D002470:Cell Survival; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D006801:Humans; D051379:Mice; D061848:Optical Imaging / Q000379:methods*; D013481:Superoxides / Q000378:metabolism*
D013481:Superoxides