A parallel increase in placental oxidative stress and antioxidant defenses occurs in pre-gestational type 1 but not gestational diabetes.
We aimed to determine the oxidative stress status in placentas obtained from gestational (GDM) and type 1 (T1D) diabetic pregnancies. Malonaldehyde and protein carbonyls, two biomarkers of oxidative damage, were higher in T1D but not in GDM placentas. Also, higher reduced glutathione and lower oxidized glutathione levels and higher glutathione peroxidase activity were found in T1D but not in GDM placentas. These results suggest that T1D placentas may develop a protective antioxidant mechanism to overcome higher oxidative stress levels.
Department of Biochemistry (U38-FCT), Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal.
J R
JR
Araújo
C
C
Ramalho
A
A
Correia-Branco
A
A
Faria
T
T
Ferraz
E
E
Keating
F
F
Martel
D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology / Q000378:metabolism*; D016640:Diabetes, Gestational / Q000201:enzymology / Q000378:metabolism; D004351:Drug Resistance; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D016877:Oxidants / Q000494:pharmacology; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D010920:Placenta / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000201:enzymology / Q000378:metabolism*; D050050:Protein Carbonylation / Q000187:drug effects; D047929:Term Birth; D015854:Up-Regulation / Q000187:drug effects; D020122:tert-Butylhydroperoxide / Q000494:pharmacology; D000094765:Glutathione Peroxidase GPX1
D015415:Biomarkers; D016877:Oxidants; D008315:Malondialdehyde; D020122:tert-Butylhydroperoxide; D005979:Glutathione Peroxidase; D005978:Glutathione; D000094765:Glutathione Peroxidase GPX1; C000722067:GPX1 protein, human
GDM; GSX; MDA; Maternal diabetes; Oxidative stress; Placenta; T1D; gestational diabetic pregnancies; malonaldehyde; pre-gestational type 1 diabetic pregnancies; tert-BOOH; tert-butyl hydroperoxide; total glutathione