Proteomic and comparative genomic analysis of two Brassica napus lines differing in oil content.
Ultrastructural observations, combined with proteomic and comparative genomic analyses, were applied to interpret the differences in protein composition and oil-body characteristics of mature seed of two Brassica napus lines with high and low oil contents of 55.19% and 36.49%, respectively. The results showed that oil bodies were arranged much closer in the high than in the low oil content line, and differences in cell size and thickness of cell walls were also observed. There were 119 and 32 differentially expressed proteins (DEPs) of total and oil-body proteins identified. The 119 DEPs of total protein were mainly involved in the oil-related, dehydration-related, storage and defense/disease, and some of these may be related to oil formation. The DEPs involved with dehydration-related were both detected in total and oil-body proteins for high and low oil lines and may be correlated with the number and size of oil bodies in the different lines. Some genes that corresponded to DEPs were confirmed by quantitative trait loci (QTL) mapping analysis for oil content. The results revealed that some candidate genes deduced from DEPs were located in the confidence intervals of QTL for oil content. Finally, the function of one gene that coded storage protein was verified by using a collection of Arabidopsis lines that can conditionally express the full length cDNA from developing seeds of B. napus.
Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology , Wuhan 430074, China.
Lu
L
Gan
Chun-yu
CY
Zhang
Xiao-dong
XD
Wang
Hao
H
Wang
Yan
Y
Long
Yong-tai
YT
Yin
Dian-rong
DR
Li
Jian-Hua
JH
Tian
Zai-yun
ZY
Li
Zhi-wei
ZW
Lin
Long-Jiang
LJ
Yu
Mao-Teng
MT
Li
D017360:Arabidopsis; D029688:Brassica napus / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D048429:Cell Size; D018076:DNA, Complementary / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D005227:Fatty Acids / Q000032:analysis; D023281:Genomics / Q000379:methods; D005961:Glucosinolates / Q000032:analysis; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D010938:Plant Oils / Q000032:analysis*; D010940:Plant Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D040641:Quantitative Trait Loci / Q000235:genetics; D012639:Seeds / Q000737:chemistry*; D013045:Species Specificity
D018076:DNA, Complementary; D005227:Fatty Acids; D005961:Glucosinolates; D010938:Plant Oils; D010940:Plant Proteins