Enhanced anti-topoisomerase II activity by mucoadhesive 4-CBS-chitosan/poly (lactic acid) nanoparticles.
In this study, the biodegradable mucoadhesive 4-carboxybenzensulfonamide chitosan (4-CBS-chitosan)/poly (lactic acid) (PLA) nanoparticles were fabricated by the electrospray ionization technique for enhancing anti-topoisomerase II (Topo II) activity. The obtained (4-CBS-chitosan/PLA)-DOX nanoparticles were characterized using SEM, particle size analyzer. We emphasis on encapsulation efficiency, in vitro drug release behavior and also performed in vitro studies of Topo II inhibitory activity using gel electrophoresis. In addition, the cytotoxicity of the 4-CBS-chitosan/PLA nanoparticles using MTT assay was also studied. The mean particle size of spherical shaped (4-CBS-chitosan/PLA)-DOX is less than 300 nm. The DOX loaded 4-CBS-chitosan/PLA composite nanoparticles produced high entrapment efficiency of 85.8% and provided the prolonged release of DOX extended to 26 days and also still had strong Topo II inhibitory activity up to 77.4%. Overall, it was shown that 4-CBS-chitosan/PLA nanoparticles could be promising carriers for controlled delivery of anticancer drugs.
Program of Petrochemistry and Polymer Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Kultida
K
Songsurang
Phruetchika
P
Suvannasara
Chuttree
C
Phurat
Songchan
S
Puthong
Krisana
K
Siraleartmukul
Nongnuj
N
Muangsin
D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D004250:DNA Topoisomerases, Type II / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000138:chemical synthesis*; D004317:Doxorubicin / Q000494:pharmacology; D004337:Drug Carriers / Q000138:chemical synthesis*; D004339:Drug Compounding; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D019344:Lactic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009928:Organ Specificity; D010316:Particle Size; D010957:Plasmids / Q000187:drug effects; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D013449:Sulfonamides / Q000737:chemistry*; D000096926:Benzenesulfonamides
D000970:Antineoplastic Agents; D048271:Chitosan; D004250:DNA Topoisomerases, Type II; D003692:Delayed-Action Preparations; D004317:Doxorubicin; D004337:Drug Carriers; D019344:Lactic Acid; D011091:Polyesters; D011108:Polymers; D013449:Sulfonamides; C583470:4-carboxybenzenesulfonamide-chitosan; D000096926:Benzenesulfonamides; C033616:poly(lactide)
4-Carboxybenzenesulfonamide–chitosan/poly(lactic acid) nanoparticles; Electrospray ionization; Topoisomerase II inhibition