A highly selective ratiometric visual and red-emitting fluorescent dual-channel probe for imaging fluoride anions in living cells.
Recently, growing attention has been paid to the accurate determination of fluoride anion (F(-)) in the environment and living systems for its toxicity and biological function investigation. In this paper, we developed a ratiometric visual and red-emitting fluorescent dual-channel probe (1) employed Si-O bond as a highly selective recognition receptor for imaging F(-) in living cells. Probe 1 possesses a potential internal charge transfer (ICT) structure, and displays a large (158 nm) red-shifted absorption spectrum and the color changes from yellow to blue upon addition of F(-) in the aqueous solution. In addition, probe 1 can be used to detect F(-) quantitatively by the ratiometric absorption and turn-on fluorescence spectroscopy methods with excellent sensitivity. Finally, the results of its application to bioimaging of F(-) in living cells show that probe 1 would be of great benefit to biomedical researchers for investigating the effects of fluoride in biological systems.
School of Resources and Environment, University of Jinan, Shandong Provincial Engineering Technology Research Center for Ecological Carbon Sink and Capture Utilization, Jinan 250022, China. Electronic address: lcyzbc@163.com.
Baocun
B
Zhu
He
H
Kan
Jingkai
J
Liu
Hanqing
H
Liu
Qin
Q
Wei
Bin
B
Du
D000042:Absorption; D000838:Anions / Q000737:chemistry / Q000302:isolation & purification*; D015374:Biosensing Techniques; D058948:Cell Tracking; D005456:Fluorescent Dyes / Q000737:chemistry; D005459:Fluorides / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D013050:Spectrometry, Fluorescence
D000838:Anions; D005456:Fluorescent Dyes; D005459:Fluorides
Bioimaging; Fluoride; Ratiometric detection; Red-emitting fluorescence; Visual probe