Active peptides from skate (Okamejei kenojei) skin gelatin diminish angiotensin-I converting enzyme activity and intracellular free radical-mediated oxidation.
Skin gelatin of skate (Okamejei kenojei) was hydrolyzed using Alcalase, flavourzyme, Neutrase and protamex. It was found that the Alcalase hydrolysate exhibited the highest angiotensin-I converting enzyme (ACE) inhibitory activity. Then, Alcalase hydrolysate was further hydrolyzed with protease and separated by an ultrafiltration membrane system. Finally, two peptides responsible for ACE inhibitory activity were identified to be MVGSAPGVL (829Da) and LGPLGHQ (720Da), with IC50 values of 3.09 and 4.22μM, respectively. Moreover, the free radical-scavenging activity of the purified peptides was determined in human endothelial cells. In addition, the antioxidative mechanism of the purified peptides was evaluated by protein and gene expression levels of antioxidant enzymes. The current study demonstrated that the peptides derived from skate skin gelatin could be used in the food industry as functional ingredients with potent antihypertensive and antioxidant benefits.
Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of Korea.
Dai-Hung
DH
Ngo
BoMi
B
Ryu
Se-Kwon
SK
Kim
D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D000959:Antihypertensive Agents / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival; D029941:Fish Proteins / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000494:pharmacology; D005780:Gelatin / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D007703:Peptidyl-Dipeptidase A / Q000737:chemistry; D011492:Protein Hydrolysates / Q000737:chemistry / Q000494:pharmacology; D012860:Skates, Fish; D012867:Skin / Q000737:chemistry*; D013381:Subtilisins / Q000737:chemistry
D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D029941:Fish Proteins; D016166:Free Radical Scavengers; D010455:Peptides; D011492:Protein Hydrolysates; D005780:Gelatin; D007703:Peptidyl-Dipeptidase A; D013381:Subtilisins
ACE inhibition; Antioxidative enzymes; Bioactive peptides; Human endothelial; ROS; Skate skin