Low-dose nitrite alleviates early effects of an X-ray contrast medium on renal hemodynamics and oxygenation in rats.
OBJECTIVE
Renal tissue hypoperfusion and hypoxia are pivotal pathophysiological elements in contrast media (CM)-induced acute kidney injury (AKI). According to a new paradigm of hypoxic vasodilation, nitrite is reduced to vasodilatory nitric oxide by hemoglobin in hypoxic areas. Here, we study the potential of low-dose nitrite administration to alleviate CM-induced AKI.


METHODS
In anesthetized rats, arterial blood pressure, total renal blood flow, cortical and medullary erythrocyte flux and oxygen tension (pO2), hindquarter blood flow, and methemoglobin were measured. Nitrite was continuously infused (0.172 mg/h per kilogram of body mass for 10 minutes; thereafter, 0.057 mg/h per kilogram of body mass); control rats received isotonic saline. Hypoxic vasodilation was studied through a brief period of hypoxia (10% inspiratory oxygen fraction). Then, 1.5 mL of the CM, iodixanol of 320 mg I/mL, was injected into the thoracic aorta and the effects studied for 60 minutes.


RESULTS
Nitrite infusion slightly increased methemoglobin, but it did not change per se any other parameter including arterial pressure. However, nitrite enhanced the hypoxic vasodilation in the kidney and hindquarter (by 100% and 70%, respectively) during the brief hypoxic challenge. In the control rats, CM resulted in renal tissue hypoxia mainly because of renal vasoconstriction. Medullary and cortical pO2 dropped upon CM injection and remained 30% to 40% below pre-CM level throughout the observation period. Nitrite infusion improved renal tissue oxygenation mainly by hindering CM-induced renal vasoconstriction. After a small transient drop (<25% for <5 minutes), medullary and cortical pO2 was restored to values indistinguishable from pre-CM levels.


CONCLUSIONS
Low-dose nitrite infusion greatly alleviates early adverse effects of CM on renal tissue oxygenation in rats. Nitrite's beneficial effect probably relies on its reduction to nitric oxide in hypoxic tissue with ensuing vasodilation. Our nitrite dose resulted in a negligible increase in methemoglobin and did not induce hypotension. Thus, low-dose nitrite infusion might prove to be a reasonably specific measure to reduce the risk for CM-induced AKI.
From the *Institut für Vegetative Physiologie, Center for Cardiovascular Research, Charité-Universitätsmedizin Berlin, Campus Mitte; and †Berlin Ultrahigh Field Facility, Max Delbrück Center for Molecular Medicine, Berlin, Germany.
Erdmann
E
Seeliger
Kathleen
K
Cantow
Karen
K
Arakelyan
Mechthild
M
Ladwig
Pontus B
PB
Persson
Bert
B
Flemming
D058186:Acute Kidney Injury / Q000139:chemically induced* / Q000503:physiopathology* / Q000517:prevention & control; D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects; D003287:Contrast Media / Q000009:adverse effects; D004305:Dose-Response Relationship, Drug; D007668:Kidney / Q000187:drug effects / Q000503:physiopathology*; D008297:Male; D009573:Nitrites / Q000008:administration & dosage*; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012079:Renal Circulation / Q000187:drug effects*; D014057:Tomography, X-Ray Computed / Q000009:adverse effects; D016896:Treatment Outcome; D014283:Triiodobenzoic Acids / Q000009:adverse effects*
D003287:Contrast Media; D009573:Nitrites; D014283:Triiodobenzoic Acids; C044834:iodixanol; D010100:Oxygen