Inhibition of oxidative stress by low-molecular-weight polysaccharides with various functional groups in skin fibroblasts.
The aim of this study was to evaluate the in cellulo inhibition of hydrogen-peroxide-induced oxidative stress in skin fibroblasts using different low-molecular-weight polysaccharides (LMPS) prepared from agar (LMAG), chitosan (LMCH) and starch (LMST), which contain various different functional groups (i.e., sulfate, amine, and hydroxyl groups). The following parameters were evaluated: cell viability, intracellular oxidant production, lipid peroxidation, and DNA damage. Trolox was used as a positive control in order to allow comparison of the antioxidant efficacies of the various LMPS. The experimentally determined attenuation of oxidative stress by LMPS in skin fibroblasts was: LMCH > LMAG > LMST. The different protection levels of these LMPS may be due to the physic-chemical properties of the LMPS' functional groups, including electron transfer ability, metal ion chelating capacities, radical stabilizing capacity, and the hydrophobicity of the constituent sugars. The results suggest that LMCH might constitute a novel and potential dermal therapeutic and sun-protective agent.
Department of Food Science, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung 20224, Taiwan. tml@mail.ntou.edu.tw.
Szu-Kai
SK
Chen
Chu-Hsi
CH
Hsu
Min-Lang
ML
Tsai
Rong-Huei
RH
Chen
Gregor P C
GP
Drummen
D000975:Antioxidants / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects; D004579:Electron Transport / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D057927:Hydrophobic and Hydrophilic Interactions / Q000187:drug effects; D007502:Iron Chelating Agents / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008970:Molecular Weight; D018384:Oxidative Stress / Q000187:drug effects*; D011134:Polysaccharides / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects* / Q000378:metabolism
D000975:Antioxidants; D005609:Free Radicals; D007502:Iron Chelating Agents; D011134:Polysaccharides; D006861:Hydrogen Peroxide