The oral administration of trans-caryophyllene attenuates acute and chronic pain in mice.
Trans-caryophyllene is a sesquiterpene present in many medicinal plants' essential oils, such as Ocimum gratissimum and Cannabis sativa. In this study, we evaluated the antinociceptive activity of trans-caryophyllene in murine models of acute and chronic pain and the involvement of trans-caryophyllene in the opioid and endocannabinoid systems. Acute pain was determined using the hot plate test (thermal nociception) and the formalin test (inflammatory pain). The chronic constriction injury (CCI) of the sciatic nerve induced hypernociception was measured by the hot plate and von Frey tests. To elucidate the mechanism of action, mice were pre-treated with naloxone or AM630 30 min before the trans-caryophyllene treatment. Afterwards, thermal nociception was evaluated. The levels of IL-1β were measured in CCI-mice by ELISA. Trans-caryophyllene administration significantly minimized the pain in both the acute and chronic pain models. The antinociceptive effect observed during the hot plate test was reversed by naloxone and AM630, indicating the participation of both the opioid and endocannabinoid system. Trans-caryophyllene treatment also decreased the IL-1β levels. These results demonstrate that trans-caryophyllene reduced both acute and chronic pain in mice, which may be mediated through the opioid and endocannabinoid systems.
Departamento de Psicobiologia, Universidade Federal de São Paulo, Brazil. Electronic address: lyviabiologia@gmail.com.
L I G
LI
Paula-Freire
Departamento de Psicobiologia, Universidade Federal de São Paulo, Brazil.
M L
ML
Andersen
Faculdade de Farmácia, Universidade São Judas Tadeu, São Paulo, Brazil.
V S
VS
Gama
Departamento de Psicobiologia, Universidade Federal de São Paulo, Brazil.
G R
GR
Molska
Departamento de Medicina Preventiva, Universidade Federal de São Paulo, Brazil.
E L A
EL
Carlini
D059787:Acute Pain / Q000188:drug therapy*; D000284:Administration, Oral; D000700:Analgesics / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D002188:Cannabis / Q000737:chemistry; D059350:Chronic Pain / Q000188:drug therapy*; D005557:Formaldehyde; D006358:Hot Temperature; D006930:Hyperalgesia / Q000188:drug therapy; D007249:Inflammation / Q000150:complications / Q000188:drug therapy; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009270:Naloxone / Q000494:pharmacology; D031339:Ocimum / Q000737:chemistry; D009822:Oils, Volatile / Q000737:chemistry; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes / Q000494:pharmacology / Q000627:therapeutic use*
D000700:Analgesics; D053583:Interleukin-1beta; D009822:Oils, Volatile; D010936:Plant Extracts; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; D005557:Formaldehyde; D009270:Naloxone; C024714:caryophyllene
Endocannabinoid system; Interleukin 1β; Medicinal plants; Opioid system; Pain; Trans-caryophyllene