Effect of Sophora subprosrate polysaccharide on oxidative stress induced by PCV2 infection in RAW264.7 cells.
In this study, an oxidative stress model was first developed in a mouse macrophage cell line (RAW264.7 cells) by infecting the cells with porcine circovirus type 2 (PCV2). The regulatory effect of Sophora subprosrate polysaccharide (SSP) on PCV2-induced oxidative stress was investigated. The results showed that after infection with PCV2, reactive oxygen species (ROS) and nitric oxide (NO) production, myeloperoxidase (MPO) activity, and inducible nitric oxide synthase (iNOS) expression were significantly increased. Meanwhile, the ratio of reduced glutathione to oxidized glutathione (GSH/GSSG) and hydroxyl radical prevention capacity were greatly reduced. These data indicate successful creation of an oxidative stress model in RAW264.7 cells. A dramatic decrease in cell viability was observed in the cells exposed to oxidative stress compared to the control. When the cells were treated with SSP in concentrations of 100, 200 or 400 μg/mL post PCV2 infection, an increase in the GSH/GSSG ratio and hydroxyl radical prevention capacity was observed. We also observed decreased ROS and NO production, MPO activity, and iNOS expression in the infected cells. Our results demonstrated that PCV2 infection was able to induce oxidative stress in RAW264.7 cells and that SSP could reduce the negative effects resulting from the PCV2 infection.
College of Animal Science and Technology, Guangxi University, Nanning 530005, PR China.
Zi-Jie
ZJ
Su
Ying-Yi
YY
Wei
Dan
D
Yin
Xue-Hong
XH
Shuai
Yun
Y
Zeng
Ting-Jun
TJ
Hu
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D017925:Circovirus / Q000235:genetics / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors; D008264:Macrophages / Q000378:metabolism* / Q000821:virology*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D029909:Sophora / Q000737:chemistry*; D013552:Swine
D011134:Polysaccharides; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D017665:Hydroxyl Radical; D009195:Peroxidase; D052247:Nitric Oxide Synthase Type II; D005978:Glutathione; D019803:Glutathione Disulfide
Oxidative stress; Porcine circovirus type 2 (PCV2); Sophora subprosrate polysaccharide (SSP)