The effect of anti-TGF-β2 antibody functionalized intraocular lens on lens epithelial cell migration and epithelial-mesenchymal transition.
Migration and epithelial-mesenchymal transition (EMT) of lens epithelial cells (LECs) are main causes of central posterior capsule opacification after cataract extraction combined with intraocular lens (IOL) implantation. In this study, commercially available hydrophobic acrylic IOLs were first pretreated with atmospheric pressure glow discharge plasma to produce plenty of negatively charged chemical groups onto IOL surface, then polyethylenimine was deposited onto IOL surfaces as a precursor monolayer, and then anti-TGF-β2 (anti-T) antibody and poly-l-lysine were sequentially deposited onto IOL surface for four cycles followed by another upmost monolayer of anti-T antibody via layer-by-layer self-assembly technique. After the fabrication of anti-T antibody multilayers on IOL surface, the surface characteristics of the anti-T antibody functionalized IOL, as well as its effect on LECs adhesion, proliferation, migration and EMT were then tested in this study. Our results revealed that anti-T antibody multilayers could be successfully immobilized onto IOL surfaces by plasma pretreatment and layer-by-layer self-assembly technique, and could keep stable for at least 3 months on IOL surface. The anti-T antibody immobilized in the multilayers on IOL surfaces showed good immunological activity by its specific antigen-antibody interaction with exogenous TGF-β2. Anti-T antibody functionalized IOL surface was as smooth and flat as the untreated IOL surface. No difference in optical or physical properties was found between the anti-T antibody functionalized IOLs and the untreated IOLs. Compared with the untreated IOLs, the anti-T antibody functionalized IOL greatly inhibited LECs from migration and EMT, yet showed only transient inhibition to LECs adhesion and no inhibition to LECs proliferation. With these data, we demonstrate a simple, inexpensive, and feasible method to fabricate surface functionalized IOL for in situ capture and neutralization of TGF-β2 in the capsular bag, which might be a possible solution to preventing posterior capsule opacification after cataract surgery.
Eye Center, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China; Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou 310009, China.
Chuan-bin
CB
Sun
Eye Center, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China.
Wen-qi
WQ
Teng
Eye Center, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China.
Jian-tao
JT
Cui
Institute of Polymer Science, and Key Laboratory of Macromolecular Synthesis and Functionalization (Ministry of Education), Zhejiang University, Hangzhou 310027, China.
Xiao-jun
XJ
Huang
Eye Center, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China; Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou 310009, China. Electronic address: xlren@zju.edu.cn.
Ke
K
Yao
D000906:Antibodies / Q000737:chemistry* / Q000276:immunology / Q000494:pharmacology; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D006801:Humans; D007908:Lens, Crystalline / Q000737:chemistry*; D007910:Lenses, Intraocular; D056951:Photoelectron Spectroscopy; D013499:Surface Properties; D053781:Transforming Growth Factor beta2 / Q000276:immunology*
D000906:Antibodies; D053781:Transforming Growth Factor beta2
Intraocular lens; Layer-by-layer self assembly; Posterior capsule opacification; Surface modification; TGF-β(2) antibody