Mucosal genetic immunization through microsphere-based oral carriers.
Polymeric carriers in the form of cellulose acetate phthalate (CAP) and alginate (ALG) microspheres were used for encapsulation of plasmid DNA for oral mucosal immunization. Access into the intestinal mucosa by pVAX1 eukaryotic expression plasmid vectors carrying gene-coding sequences, either for the cholera enterotoxin B subunit (ctxB) immunostimulatory antigen or the green fluorescent protein (GFP), delivered from both types of microsphere carriers were examined in orally immunized BALB/c mice. Demonstration of transgene protein expression and IgA antibody responses at local mucosal sites suggest immunological response to a potential oral DNA vaccine formulated within the microsphere carriers.
Genetic Medicine Research Centre; Universiti Putra Malaysia; Selangor, Malaysia; Medical Genetics Laboratory; Faculty of Medicine and Health Sciences; Universiti Putra Malaysia; Selangor, Malaysia.
Rozita
R
Rosli
Medical Genetics Laboratory; Faculty of Medicine and Health Sciences; Universiti Putra Malaysia; Selangor, Malaysia.
Nadine
N
Nograles
Medical Genetics Laboratory; Faculty of Medicine and Health Sciences; Universiti Putra Malaysia; Selangor, Malaysia.
Aimi
A
Hanafi
Department of Medical Microbiology and Parasitology; Faculty of Medicine and Health Sciences; Universiti Putra Malaysia; Selangor, Malaysia.
Mariana
M
Nor Shamsudin
Genetic Medicine Research Centre; Universiti Putra Malaysia; Selangor, Malaysia; Medical Genetics Laboratory; Faculty of Medicine and Health Sciences; Universiti Putra Malaysia; Selangor, Malaysia.
Syahril
S
Abdullah
D000284:Administration, Oral; D000818:Animals; D002772:Cholera Toxin / Q000235:genetics / Q000276:immunology; D016503:Drug Delivery Systems; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000276:immunology; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D019076:Transgenes; D014611:Vaccination / Q000379:methods*; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology
D007070:Immunoglobulin A; D019444:Vaccines, DNA; D049452:Green Fluorescent Proteins; D002772:Cholera Toxin
IgA; alginate; cellulose acetate phthalate; encapsulation; microspheres; mucosal immunization; plasmid DNA