Comparison of the Simplexa FluA/B & RSV direct assay and laboratory-developed real-time PCR assays for detection of respiratory virus.
The results of the Focus Simplexa FluA/B & RSV Direct assay were compared to those of laboratory-developed reverse transcription PCR tests for 498 nasopharyngeal swabs. Concordance rates were 96.6% (476/493; κ = 0.91), 97.6% (481/493; κ = 0.47), and 99.2% (488/492; κ = 0.94) for influenza A, influenza B, and respiratory syncytial virus, respectively.
Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington, USA.
Mitchell W
MW
Woodberry
Rohit
R
Shankar
Anne
A
Cent
Keith R
KR
Jerome
Jane
J
Kuypers
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D009980:Influenza A virus / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009305:Nasopharynx / Q000821:virology; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis* / Q000821:virology; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification*; D014773:Virology / Q000379:methods*; D055815:Young Adult