In vivo stimulation of bone formation by aluminum and oxygen plasma surface-modified magnesium implants.
A newly developed magnesium implant is used to stimulate bone formation in vivo. The magnesium implant after undergoing dual aluminum and oxygen plasma implantation is able to suppress rapid corrosion, leaching of magnesium ions, as well as hydrogen gas release from the biodegradable alloy in simulated body fluid (SBF). No released aluminum is detected from the SBF extract and enhanced corrosion resistance properties are confirmed by electrochemical tests. In vitro studies reveal enhanced growth of GFP mouse osteoblasts on the aluminum oxide coated sample, but not on the untreated sample. In addition to that a small amount (50 ppm) of magnesium ions can enhance osteogenic differentiation as reported previously, our present data show a low concentration of hydrogen can give rise to the same effect. To compare the bone volume change between the plasma-treated magnesium implant and untreated control, micro-computed tomography is performed and the plasma-treated implant is found to induce significant new bone formation adjacent to the implant from day 1 until the end of the animal study. On the contrary, bone loss is observed during the first week post-operation from the untreated magnesium sample. Owing to the protection offered by the Al2O3 layer, the plasma-treated implant degrades more slowly and the small amount of released magnesium ions stimulate new bone formation locally as revealed by histological analyses. Scanning electron microscopy discloses that the Al2O3 layer at the bone-implant interface is still present two months after implantation. In addition, no inflammation or tissue necrosis is observed from both treated and untreated implants. These promising results suggest that the plasma-treated magnesium implant can stimulate bone formation in vivo in a minimal invasive way and without causing post-operative complications.
Department of Orthopaedics and Traumatology, The University of Hong Kong, Pokfulam, Hong Kong, China; Shenzhen Key Laboratory for Innovative Technology in Orthopaedic Trauma, The University of Hong Kong Shenzhen Hospital, 1 Haiyuan 1st Road, Futian District, Shenzhen, China.
Hoi Man
HM
Wong
Ying
Y
Zhao
Vivian
V
Tam
Shuilin
S
Wu
Paul K
PK
Chu
Yufeng
Y
Zheng
Michael Kai Tsun
MK
To
Frankie K L
FK
Leung
Keith D K
KD
Luk
Kenneth M C
KM
Cheung
Kelvin W K
KW
Yeung
D000537:Aluminum Oxide / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004563:Electrochemistry; D008274:Magnesium / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D019736:Prostheses and Implants; D055114:X-Ray Microtomography
D008274:Magnesium; D000537:Aluminum Oxide
Cyto-compatibility; Magnesium implant; Osteoblast; Plasma surface treatment