[Identification of methylation at lysine 27 and 36 sites in histone H3].
We combined high performance liquid chromatography and tandem mass spectrometry (HPLC-MS/MS) with bioinformatics tools to analyze the isobaric modified peptides which were methylated and dimethylated at either lysine (K) 27 or/and K36 from histone H3. They were identified and dissected through alignment of every fragment ion, and the two modified sites were further analyzed according to their relative intensities of MS/MS spectra.
State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China.
Guojuan
G
Wang
Kai
K
Zhang
Xiwen
X
He
Yukui
Y
Zhang
D002853:Chromatography, Liquid; D006657:Histones / Q000737:chemistry*; D008239:Lysine / Q000737:chemistry*; D008745:Methylation; D053719:Tandem Mass Spectrometry
D006657:Histones; D008239:Lysine