Chromatographic resolution of closely related species: separation of warfarin and hydroxylated isomers.
Recent developments in the field of organic synthesis are leading to increasingly complex mixtures of closely related species (positional isomers, regioisomers, diastereomers, etc.) that often prove challenging for chromatographic analysis and separation. In this study we investigate the separation of a representative mixture of warfarin and 5 different monohydroxylation isomers to assess whether conventional techniques are suitable for addressing this separation challenge, or whether 'next generation' separation tools such as multidimensional chromatography may be required. In this example, modifications of results obtained from conventional achiral and chiral chromatography method development screening platforms afford rapid separation of all components for both achiral and chiral analysis, with supercritical fluid chromatography showing the best performance in both cases (1.8min for separation of six components by achiral SFC and 8.0min for separation of twelve components by chiral SFC). While other more complex mixtures may require additional tools, these results suggest that new applications of existing separation platforms may be useful for creating the chromatographic methods required to support this new area of synthetic chemistry.
Department of Analytical Chemistry, Merck Research Laboratories, Rahway, NJ 07065, USA. Electronic address: erik.regalado@merck.com.
Erik L
EL
Regalado
Wes
W
Schafer
Ray
R
McClain
Christopher J
CJ
Welch
D000925:Anticoagulants / Q000737:chemistry / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods*; D006900:Hydroxylation; D007536:Isomerism; D014859:Warfarin / Q000737:chemistry / Q000302:isolation & purification*
D000925:Anticoagulants; D014859:Warfarin
Chiral screening; Complex mixtures; High-throughput experimentation; Method development; SFC; UPLC