[Protective mechanism of cerebrospinal fluid containing qingxin kaiqiao recipe on PC12 cell injury induced by glutamate].
OBJECTIVE
To study the protective effect of cerebrospinal fluid containing Qingxin Kaiqiao recipe on PC12 cell injury induced by glutamate (Glu), in order to provide basis for the conical application of the recipe.


METHODS
SD rats were orally administered with decoction of Qingxin Kaiqiao recipe (7.9 g x kg(-1)) for three and a half days, 2 times a day, in order to prepare cerebrospinal fluid containing Qingxin Kaiqiao recipe. PC cells were divided into the normal group, the model group, the nimodipine group, the 10% normal CSF group, the 10% medicated CSF group, the 20% normal CSF group, the 20% medicated CSF group. Except for the normal group, other groups were cultured with PC12 cells and Glu with the final concentration of 20 mmol x L(-1) to establish the nerve cell injury model. Apart from the model group and the normal group, other groups were intervened with nimodipine, normal cerebrospinal fluid, and 10% and 20% medicated CSF. RT-PCR was used to detect the expression level of Bax mRNA, Bcl-2 mRNA and Caspase-3 mRNA, and MTT method was used to detect the activity of PC12 cells.


RESULTS
The activity of PC12 cells of all of medicated CSF groups was higher than that of the model group, with the decrease in the expression of Bax mRNA and Caspase-3 mRNA and the increase in the expression of Bcl-2 mRNA. They showed a significant different with the model group (P < 0.01). The 20% medicated CSF group was superior than the 10% medicated CSF group (P < 0.01).


CONCLUSIONS
Qingxin Kaiqiao recipe shows an apparent protective effect on PC12 cells injured by Glu.
The Second Clinical College of Wenzhou Medical College, Wenzhou 325003, China. wyzhongyixi@163.com
Hai-Yan
HY
Hu
Dong-Ze
DZ
Xi
Lei
L
Lei
Xiang
X
Chen
Wen-Hua
WH
Wang
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000235:genetics; D002555:Cerebrospinal Fluid; D018698:Glutamic Acid / Q000633:toxicity*; D008297:Male; D008516:Medicine, Chinese Traditional; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D018696:Neuroprotective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D018698:Glutamic Acid; D053148:Caspase 3