Rate-adjusted cross-linking reaction by photoresponsive α-bromoaldehyde (PBA)-conjugated ODN.
We developed a photoresponsive α-bromoaldehyde-conjugated oligonucleotide (PBA-ODN). The PBA-ODN selectively reacted and formed covalent bonds with target oligonucleotides having adenine or cytosine at the frontal position of the aldehyde derivative. Kinetic studies revealed that PBA-ODN has increased kinetic rates for the formation of cross-linked duplexes compared with the corresponding α-chloroaldehyde-conjugated oligonucleotide (PCA-ODN).
Kyoto Institute of Technology, Graduate School of Science and Technology, Department of Biomolecular Engineering, Kyoto, Japan. Electronic address: akobori@kit.ac.jp.
Akio
A
Kobori
Yuko
Y
Nagae
Yuta
Y
Sugihara
Asako
A
Yamayoshi
Akira
A
Murakami
D000447:Aldehydes / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D054879:Halogenation; D007700:Kinetics; D009841:Oligonucleotides / Q000737:chemistry*
D000447:Aldehydes; D003432:Cross-Linking Reagents; D009841:Oligonucleotides
Antisense; Cross-link; α-Haloaldehyde