Permeabilized probiotic Lactobacillus plantarum as a source of β-galactosidase for the synthesis of prebiotic galactooligosaccharides.
Permeabilized probiotic Lactobacillus plantarum was used as a source of β-galactosidase for the synthesis of galactooligosaccharides (GOS) from lactose. β-galactosidase activity was highest when galactose (1,724 Miller Units) was used as a carbon source compared to lactose, sucrose or glucose at 37 °C, 18 h. Permeabilized cells had the highest transgalactosylation activity resulting in 34 % (w/w) GOS synthesis from 40 % (w/v) lactose at 50 °C over 12 h. HPLC revealed that the GOS were composed of 13 % disaccharides (non-lactose), 17 % trisaccharides and 4 % tetrasaccharides that were further confirmed by ESI–MS.
Duraiswamy
D
Gobinath
Siddalingaiya Gurudutt
SG
Prapulla
D002463:Cell Membrane Permeability; D005690:Galactose / Q000737:chemistry / Q000378:metabolism*; D048191:Lactiplantibacillus plantarum / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D056692:Prebiotics; D019936:Probiotics / Q000737:chemistry / Q000378:metabolism*; D001616:beta-Galactosidase / Q000737:chemistry / Q000378:metabolism*
D009844:Oligosaccharides; D056692:Prebiotics; D001616:beta-Galactosidase; D005690:Galactose