Nematicidal activity of mint aqueous extracts against the root-knot nematode Meloidogyne incognita.
The nematicidal activity and chemical characterization of aqueous extracts and essential oils of three mint species, namely, Mentha × piperita , Mentha spicata , and Mentha pulegium , were investigated. The phytochemical analysis of the essential oils was performed by means of GC-MS, whereas the aqueous extracts were analyzed by LC-MS. The most abundant terpenes were isomenthone, menthone, menthol, pulegone, and carvone, and the water extracts yielded mainly chlorogenic acid, salvianolic acid B, luteolin-7-O-rutinoside, and rosmarinic acid. The water extracts exhibited significant nematicidal activity against Meloidogyne incognita , and the EC50/72h values were calculated at 1005, 745, and 300 mg/L for M. × piperita, M. pulegium, and M. spicata, respectively. Only the essential oil from M. spicata showed a nematicidal activity with an EC50/72h of 358 mg/L. Interestingly, menthofuran and carvone showed EC50/48h values of 127 and 730 mg/L, respectively. On the other hand, salicylic acid, isolated in the aqueous extracts, exhibited EC50 values at 24 and 48 h of 298 ± 92 and 288 ± 79 mg/L, respectively.
Department of Life and Environmental Sciences, University of Cagliari , via Ospedale 72, 09124 Cagliari, Italy.
Pierluigi
P
Caboni
Marco
M
Saba
Graziella
G
Tocco
Laura
L
Casu
Antonio
A
Murgia
Andrea
A
Maxia
Urania
U
Menkissoglu-Spiroudi
Nikoletta
N
Ntalli
D000818:Animals; D000969:Antinematodal Agents / Q000737:chemistry / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D027541:Mentha / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D014431:Tylenchoidea / Q000187:drug effects*
D000969:Antinematodal Agents; D009822:Oils, Volatile; D010936:Plant Extracts