[Effect of propofol on thrombospondin-1 expression in cultured newborn rat cortical astrocytes].
OBJECTIVE
To investigate the effect of propofol on the expression of thrombospondin-1 (THBS-1) mRNA and protein in purified newborn rat cortical astrocytes in vitro.


METHODS
Astrocytes were isolated from newborn rat cortex and grown in culture before exposure to propofol at 3, 10, 30, 100 or 300 µmol/L for 6 h, 12, or 24 h. The mRNA level of THBS-1 was detected by RT-PCR, and the protein level of THBS-1 was detected by immunofluorescence cytochemistry and Western blotting.


RESULTS
Propofol exposure caused significantly upregulated THBS-1 level in cultured astrocytes (P<0.05) to a level about 1.3 times higher than that in control cells. The mRNA and protein levels of THBS-1 in cultured rat cortical astrocytes were upregulated by exposures to 10, 30 and 100 µmol/L propofol (P<0.01). High expression of THBS-1 mRNA and protein was detected in the cells with exposures for different durations (P<0.05), especially in the 12 h group (P<0.01).


CONCLUSIONS
Propofol at clinically relevant concentrations can modulate the level of THBS-1 secreted by astrocytes of rat cerebral cortex in vitro.
Department of Anesthesiology, Peiking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.E-mial: squirrelyuki@126.com.
Yu
Y
Zhang
Yu
Y
Li
Weiguang
W
Li
Chenggang
C
Zhang
Tiehu
T
Ye
D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D015742:Propofol / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019700:Thrombospondin 1 / Q000378:metabolism*
D019700:Thrombospondin 1; C582391:Thbs1 protein, rat; D015742:Propofol