Ameliorative effect of ferulic acid against renal injuries mediated by nuclear factor-kappaB during glycerol-induced nephrotoxicity in Wistar rats.
The pathogenesis of glycerol-induced myoglobinuric acute renal failure involves ischemia, vascular congestion and reactive oxygen metabolites. In this study, we have investigated for the first time, the role of ferulic acid in attenuating glycerol-induced nephrotoxicity. Male Wistar rats were injected intramuscularly with 8 mL/kg body weight of 50% glycerol, glycerol + ferulic acid at the dose of 5, 10, 15, 20 and 25 mg/kg body weight. After 24 h, the rats were sacrificed and the kidneys were removed for histological and immunohistochemical studies. Furthermore, determinations of lipid peroxidation (LPO) as well as antioxidant enzymes were also analyzed; blood, urine samples were collected in order to quantify renal function and nitric oxide generation, respectively. Glycerol-induced rats showed a significant increase in the level of urinary markers assessed in serum as well as kidney and these were reversed upon ferulic acid treatment. A significant increase in urine nitric oxide, serum as well as kidney LPO, decrease in activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and reduced glutathione were observed in glycerol-induced rats. Immunohistochemical study in glycerol-induced rats demonstrated an increase in the level of nuclear factor-kappaB (NF-κB). All these effects induced by glycerol were reduced upon treatment with ferulic acid in a dose-dependent manner. To conclude, ferulic acid enhances antioxidants and decreases NF-κB, thereby protecting the cells against stress induced by glycerol.
Department of Animal Health and Management, Alagappa University , Karaikudi, Tamil Nadu , India .
Ramar
R
Manikandan
Manikandan
M
Beulaja
Raman
R
Thiagarajan
Mohan
M
Pandi
Chinnasamy
C
Arulvasu
Narayanan Marimuthu
NM
Prabhu
Rajendran
R
Saravanan
Masanam
M
Esakkirajan
Subramanian
S
Palanisamy
Ganeshan
G
Dhanasekaran
Rajagopalan Girijakumari
RG
Nisha
Kasinathan
K
Devi
Malaikannan
M
Latha
D058186:Acute Kidney Injury / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D002374:Catalase / Q000378:metabolism; D003373:Coumaric Acids / Q000494:pharmacology*; D003404:Creatinine / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D005990:Glycerol; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D009569:Nitric Oxide / Q000652:urine; D018384:Oxidative Stress / Q000187:drug effects*; D010944:Plants; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D014527:Uric Acid / Q000378:metabolism
D003373:Coumaric Acids; D016328:NF-kappa B; D014527:Uric Acid; D009569:Nitric Oxide; C004999:ferulic acid; D003404:Creatinine; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005978:Glutathione; D005990:Glycerol