Dynamic neuroanatomy at subcellular resolution in the zebrafish.
Genetic means to visualize and manipulate neuronal circuits in the intact animal have revolutionized neurobiology. "Dynamic neuroanatomy" defines a range of approaches aimed at quantifying the architecture or subcellular organization of neurons over time during their development, regeneration, or degeneration. A general feature of these approaches is their reliance on the optical isolation of defined neurons in toto by genetically expressing markers in one or few cells. Here we use the afferent neurons of the lateral line as an example to describe a simple method for the dynamic neuroanatomical study of axon terminals in the zebrafish by laser-scanning confocal microscopy.
Département de Physiologie, Institut de Génomique Fonctionnelle, CNRS UMR 5203, INSERM U661, Universtités Montpellier 1 & 2, Montpellier, France.
Adèle
A
Faucherre
Hernán
H
López-Schier
D000818:Animals; D001369:Axons / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D021621:Imaging, Three-Dimensional; D042541:Intracellular Space / Q000378:metabolism*; D008164:Luminescent Proteins / Q000235:genetics; D009445:Neuroanatomy / Q000191:economics / Q000379:methods*; D009475:Neurons, Afferent / Q000166:cytology; D013997:Time Factors; D015027:Zebrafish / Q000033:anatomy & histology* / Q000196:embryology; D000097573:Red Fluorescent Protein
D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein