The bacterial adhesion on and the cytotoxicity of various dental cements used for implant-supported fixed restorations.
OBJECTIVE
Bacterial adhesion on and cytotoxicity of eight luting agents used for implant-supported restorations were investigated.


METHODS
Surface roughness (Ra), surface free energy (SFE) values and three-dimensional images by atomic-force microscopy of circular specimens were determined. Bacterial suspensions of Streptococcus sanguinis and Streptococcus epidermidis were incubated at 37°C for 2 h. Adhering bacteria were examined with fluorescence dye CytoX-Violet, stained with 4',6-diamidino-2-phenylindole (DAPI) and visualized by fluorescence-microscopy. Cytotoxicity-testing was done with WST-1-tests (water soluble tetrazolium). No significant differences, neither with regard to Ra nor regarding SFE were determined.


RESULTS
Adherence of S. sanguinis was less on titanium, TempBondNE and TempBond. TempBond, TempBondNE, RelyX Unicem and Implantlink Semi Classic presented low amounts of S. epidermidis. WST-testing showed high cytotoxic potential of Harvard, Aqualox, TempBondNE and TempBond. No combination of low adherent bacteria with low cytotoxicity was found.


CONCLUSIONS
From a biological in-vitro perspective, none of the cements may be recommended for implant-supported restorations.
Department of Prosthetic Dentistry, Regensburg University Medical Center, Regensburg, Germany.
Cornelia
C
Winkler
Lina
L
Schäfer
Oliver
O
Felthaus
Juri
J
Allerdings
Sebastian
S
Hahnel
Michael
M
Behr
Ralf
R
Bürgers
D001422:Bacterial Adhesion; D018441:Biofilms; D003738:Dental Cements; D015921:Dental Implants; D003793:Dental Restoration, Permanent; D008856:Microscopy, Fluorescence; D013212:Staphylococcus epidermidis / Q000302:isolation & purification
D003738:Dental Cements; D015921:Dental Implants