The effect of nonuniform magnetic targeting of intracoronary-delivering mesenchymal stem cells on coronary embolisation.
Magnetic targeting has been recently introduced to enhance cell retention in animals with acute myocardial infarction. However, it is unclear whether the magnetic accumulation of intravascular cells increases the risk of coronary embolism. Upon finite element analysis, we found that the permanent magnetic field was nonuniform, manifestated as attenuation along the vertical axis and polarisation along the horizontal axis. In the in vitro experiments, iron-labelled mesenchymal stem cells (MSCs) were accumulated in layers predominantly at the edge of the magnet. In an ischaemic rat model subjected to intracavitary MSCs injection, magnetic targeting induced unfavourable vascular embolisation and an inhomogeneous distribution of the donor cells, which prevented the enhanced cell retention from translating into additional functional benefit. These potential complications of magnetic targeting should be thoroughly investigated and overcome before clinical application.
Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, China.
Zheyong
Z
Huang
Yunli
Y
Shen
Ning
N
Pei
Aijun
A
Sun
Jianfeng
J
Xu
Yanan
Y
Song
Gangyong
G
Huang
Xiaoning
X
Sun
Shuning
S
Zhang
Qing
Q
Qin
Hongming
H
Zhu
Shan
S
Yang
Xiangdong
X
Yang
Yunzeng
Y
Zou
Juying
J
Qian
Junbo
J
Ge
D000818:Animals; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000166:cytology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D053758:Nanoparticles / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology
B; CE; Coronary artery; Embolisation; F(r); I/R; IC; MNCs; MR; MSCs; MagMSCs; Magnetic targeting; Nanoparticle; NdFeB; PLL; SPIO; SRY; Stem cell; capture efficiency; intracoronary injection; ischemia/reperfusion; magnetic field density; magnetic field gradient in r direction; magnetic force on cells in r direction; magnetic resonance; magnetically-loaded mesenchymal stem cells; mesenchymal stem cells; mononuclear cells; neodymium–iron–boron; poly-l-lysine; sex-determining region Y gene; superparamagnetic oxide nanoparticles; ∂B/∂r