Identification and expression analysis of the leukocyte cell-derived chemotaxin-2 (LECT2) gene in duck (Anas platyrhynchos).
Leukocyte cell-derived chemotaxin 2 (LECT2), first identified as a chemotactic factor, is involved in the regulation of liver regeneration, carcinogenesis, and natural killer T-cell homeostasis in mammals. The function of LECT2 in the duck remains unclear, however. A suppression subtractive cDNA library was constructed from the livers of 3-day-old ducklings treated with duck hepatitis virus type I (DHV-1). A total of 66 expressed sequence tags (ESTs) were identified in the libraries. Among the novel gene fragments identified was the LECT2 gene. Full-length duck LECT2 (duLECT2) complementary DNA (cDNA) was obtained using the reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of the cDNA ends (RACE). The cDNA consisted of a 50 nucleotide 5' untranslated region (UTR), an 84 nucleotide 3' UTR, and a 1020 nucleotide open reading frame encoding a single protein of 339 amino acids. In agreement with a previously reported LECT2 sequence, the predicted amino acid sequence contains characteristic phosphorylation and N-glycosylation sites. DuLECT2 is highly similar to LECT2 genes from other vertebrates. Phylogenetic analysis demonstrated that the LECT2 gene has been highly conserved throughout vertebrate evolution. RT-PCR analyses revealed that duLECT2 mRNA is widely expressed in healthy tissues. They also showed that duLECT2 mRNA is significantly up-regulated in the liver and spleen following injection with DHV-1 or polyriboinosinic polyribocytidylic acid (poly I:C), peaking 4 or 12h post-challenge in the liver and spleen, respectively, and afterwards gradually returning to normal. Our findings suggest that duLECT2 contributes to the innate immune response against viral infections.
Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, People's Republic of China.
Qi
Q
Xu
Yang
Y
Chen
Yi Yu
YY
Tong
Zheng Yang
ZY
Huang
Wen Ming
WM
Zhao
Xiu Jun
XJ
Duan
Yang
Y
Zhang
Xiu
X
Li
Guo Bin
GB
Chang
Guo Hong
GH
Chen
D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D004372:Ducks / Q000235:genetics*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D036341:Intercellular Signaling Peptides and Proteins
DHV-1; Duck; ESTs; GAPDH; Gene expression; LECT2; NKT; ORF; RACE; RT-PCR; SSH; UTR; cDNA; complementary DNA; duLECT2; expressed sequence tags; glyceraldehyde-3-phosphate dehydrogenase; leukocyte cell-derived chemotaxin 2; natural killer T; open reading frame; poly I:C; polyriboinosinic polyribocytidylic acid; rapid amplification of the cDNA ends; reverse transcription-polymerase chain reaction; suppression subtractive hybridization; untranslated region