Abscisic acid represses the transcription of chloroplast genes.
Numerous studies have shown effects of abscisic acid (ABA) on nuclear genes encoding chloroplast-localized proteins. ABA effects on the transcription of chloroplast genes, however, have not been investigated yet thoroughly. This work, therefore, studied the effects of ABA (75 μM) on transcription and steady-state levels of transcripts in chloroplasts of basal and apical segments of primary leaves of barley (Hordeum vulgare L.). Basal segments consist of young cells with developing chloroplasts, while apical segments contain the oldest cells with mature chloroplasts. Exogenous ABA reduced the chlorophyll content and caused changes of the endogenous concentrations not only of ABA but also of cytokinins to different extents in the basal and apical segments. It repressed transcription by the chloroplast phage-type and bacteria-type RNA polymerases and lowered transcript levels of most investigated chloroplast genes drastically. ABA did not repress the transcription of psbD and a few other genes and even increased psbD mRNA levels under certain conditions. The ABA effects on chloroplast transcription were more pronounced in basal vs. apical leaf segments and enhanced by light. Simultaneous application of cytokinin (22 μM 6-benzyladenine) minimized the ABA effects on chloroplast gene expression. These data demonstrate that ABA affects the expression of chloroplast genes differentially and points to a role of ABA in the regulation and coordination of the activities of nuclear and chloroplast genes coding for proteins with functions in photosynthesis.
Department of Biology/Genetics, Humboldt University, Chausseestr. 117, D-10115 Berlin, Germany.
Maria V
MV
Yamburenko
Yan O
YO
Zubo
Radomíra
R
Vanková
Victor V
VV
Kusnetsov
Olga N
ON
Kulaeva
Thomas
T
Börner
D000040:Abscisic Acid / Q000494:pharmacology*; D002736:Chloroplasts / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D003583:Cytokinins / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D061125:Genes, Chloroplast / Q000235:genetics*; D001467:Hordeum / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D008027:Light; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects* / Q000528:radiation effects
D003583:Cytokinins; D010937:Plant Growth Regulators; D012333:RNA, Messenger; D018749:RNA, Plant; D000040:Abscisic Acid
Abscisic acid (ABA); Hordeum vulgare (L.); chloroplast; cytokinin; nucleus-encoded plastid RNA polymerase (NEP); photosynthesis; plastid-encoded plastid RNA polymerase (PEP); regulation of transcription; retrograde signalling; senescence.