Effect of low-fluoride dentifrices supplemented with calcium glycerophosphate on enamel demineralization in situ.
OBJECTIVE
To evaluate whether a low-fluoride dentifrice with calcium glycerophosphate (CaGP) reduced the demineralization process in situ.


METHODS
A cross-over design with four treatment phases of 7 days each was used. Ten volunteers wore palatal devices containing four blocks of bovine dental enamel. The enamel was treated (ex-vivo) with a placebo, 500 microg-F/g (500), 500 microg-F/g with 0.25%CaGP (500 CaGP), and 1,100 microg-F/g (1,100) dentifrices (twice a day/1 minute) under cariogenic challenge from sucrose solution. To evaluate mineral loss, surface and cross-sectional hardness were performed. The fluoride, calcium, and phosphorus ion concentrations from enamel and dental plaque were determined. The insoluble extracellular polysaccharide (EPS) concentrations were also analyzed. The data were submitted to ANOVA (1-way) followed by the Student-Newman-Keuls test (P < 0.05).


RESULTS
The mineral loss and EPS concentration were lowest in the 500 CaGP and 1,100 dentifrice groups. The use of the 500 CaGP and 1,100 dentifrices resulted in similar fluoride, calcium, and phosphorus concentrations in the enamel and in dental plaque (P > 0.05). The ionic activities of calcium phosphate phases for the 500 CaGP and 1,100 dentifrices were similar (P > or = 0.492). The low-fluoride dentifrice with 0.25%CaGP demonstrated efficacy similar to that of the positive control (1,100 dentifrice) with respect to in situ demineralization.
Dept. of Pediatric Dentistry and Public Health, Araçatuba School of Dentistry, Paulista State University (UNESP), Rua José Bonifácio 1193, Araçatuba, SP, CEP 16015-050, Brazil.
Jackeline Gallo
JG
do Amaral
Kikue Takebayashi
KT
Sassaki
Cleide Cristina Rodrigues
CC
Martinhon
Alberto Carlos Botazzo
AC
Delbem
D000328:Adult; D018592:Cross-Over Studies; D003802:Dentifrices; D004311:Double-Blind Method; D005459:Fluorides / Q000008:administration & dosage / Q000627:therapeutic use*; D005994:Glycerophosphates / Q000008:administration & dosage / Q000627:therapeutic use*; D006801:Humans; D017001:Tooth Demineralization / Q000188:drug therapy*; D055815:Young Adult
D003802:Dentifrices; D005994:Glycerophosphates; D005459:Fluorides