Isolation and identification of two novel attractant compounds from Chinese cockroach (Eupolyphaga sinensis Walker) by combination of HSCCC, NMR and CD techniques.
High-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of n-hextane-ethyl acetate-methanol-water (1.5:1:1.5:1, v/v/v/v) was applied to the isolation and purification of attractants from Chinese cockroach, Eupolyphaga sinensis Walker. Two new attractants with attractant activity towards the male insects were obtained from the extract sample in a one-step separation. Their purities were determined by HPLC. Subsequent MS, NMR and CD analyses have led to the characterization of (R)-3-ethyl-6,8-dihydroxy-7-methyl-3,4-dihydroisochromen-1-one (1) and (R)-6,8-dihydroxy-3,7-dimethyl-3,4-dihydroisochromen-1-one (2), two novel isocumarin type attractants. Based on these results, it is concluded that HSCCC is a viable separation method option for purifying insect attractants, while effectively maintaining the attracting activity of the isolates. This is the first attempt to apply counter-current chromatography technique to separate attractants from Chinese cockroach.
Ocean College, Zhejiang University, Hangzhou 310058, China. wubin@zju.edu.cn.
Wei
W
Jiang
Xiaodan
X
Wu
Bin
B
Wu
D063147:Animal Distribution / Q000187:drug effects; D000818:Animals; D002630:Chemotactic Factors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003058:Cockroaches / Q000737:chemistry* / Q000502:physiology; D003377:Countercurrent Distribution; D005260:Female; D007301:Insect Hormones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D049934:Isocoumarins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008297:Male; D008956:Models, Chemical; D008968:Molecular Conformation
C000592450:(R)-3-ethyl-6,8-dihydroxy-7-methyl-3,4-dihydroisochromen-1-one; C000592449:(R)-6,8-dihydroxy-3,7-dimethyl-3,4-dihydroisochromen-1-one; D002630:Chemotactic Factors; D007301:Insect Hormones; D049934:Isocoumarins