Isolation of strontium pools and isotope ratios in modern human hair.
The elements of human hair record specific information about an individual's health, diet, and surrounding environment. Strontium isotope ratios of human hair have attracted interest as they potentially record an individual's environment. Yet, separating the external environmental signals from the internal dietary indicators has remained a challenge. Here, we examined the effects of five different hair-cleaning methodologies to determine the extent that internal and external strontium signals can be isolated from human hair. In the first study of its kind, we employed an in-line strontium purification methodology and a multi-collector inductively coupled plasma mass spectrometer to obtain high-precision strontium isotope ratio of human hair and of leachates of the different washing treatments. We found that the different applications of an individual treatment removed a consistent amount of strontium from hair and that replicate analyses showed each treatment altered the strontium isotope ratios of hair consistently. A mass-balance approach was applied to demonstrate that strontium was quantitatively removed and was accounted for in either the treated hair or the leachate. We observed that strontium isotope ratio varied as a function of treatment aggressiveness so as to suggest that there was a fine-scale structuring of strontium within hair (transverse cross-sectional variations); these variations existed as differences in strontium concentrations and isotope ratios. As a result, the Sr isotope ratio of hair and hair leachates treated with the most aggressive cleaning methods reflected the isotope ratios of the interior and total exterior strontium signatures, respectively. The results of this study indicate that external environmental strontium signals can be distinguished from the internal signals and therefore permit the application of strontium isotope ratios of modern human hair for geospatial applications.
IsoForensics Inc, 421 Wakara Way, Suite 100, Salt Lake City, UT 84108, USA; University of Utah, Department of Biology, 257 South 1400 East, Salt Lake City, UT 84112, USA. Electronic address: brett@isoforensics.com.
Brett J
BJ
Tipple
Thuan
T
Chau
Lesley A
LA
Chesson
Diego P
DP
Fernandez
James R
JR
Ehleringer
D002725:Chloroform / Q000737:chemistry; D006197:Hair / Q000737:chemistry*; D006801:Humans; D006851:Hydrochloric Acid / Q000737:chemistry; D013058:Mass Spectrometry; D000432:Methanol / Q000737:chemistry; D017942:Nitric Acid / Q000737:chemistry; D013324:Strontium / Q000032:analysis*; D013325:Strontium Isotopes / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis
D013325:Strontium Isotopes; D014874:Water Pollutants, Chemical; D017942:Nitric Acid; D002725:Chloroform; D006851:Hydrochloric Acid; D000432:Methanol; D013324:Strontium
Forensics; Human hair; Isotopes; Multi-collector; Sr