Acid and rennet gels exhibit strong differences in the kinetics of milk protein digestion and amino acid bioavailability.
This study aimed at determining the kinetics of milk protein digestion and amino acid absorption after ingestion by six multi-canulated mini-pigs of two gelled dairy matrices having the same composition, similar rheological and structural properties, but differing by their mode of coagulation (acidification/renneting). Duodenal, mid-jejunal effluents and plasma samples were collected at different times during 7h after meal ingestion. Ingestion of the acid gel induced a peak of caseins and β-lactoglobulin in duodenal effluents after 20min of digestion and a peak of amino acids in the plasma after 60min. The rennet gel induced lower levels of both proteins in the duodenum (with no defined peak) as well as much lower levels of amino acids in the plasma than the acid gel. Plasma ghrelin concentrations suggested a potentially more satiating effect of the rennet gel compared to the acid gel. This study clearly evidences that the gelation process can significantly impact on the nutritive value of dairy products.
INRA, UMR1253 Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France; Agrocampus Ouest, UMR1253 Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France; Univ. Paris Sud-CNRS Supélec, UMR8506 Laboratoire des Signaux et Systèmes, F-91192 Gif-sur-Yvette, France.
Florence
F
Barbé
Olivia
O
Ménard
Yann
Y
Le Gouar
Caroline
C
Buffière
Marie-Hélène
MH
Famelart
Béatrice
B
Laroche
Steven Le
SL
Feunteun
Didier
D
Rémond
Didier
D
Dupont
D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism*; D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D012085:Chymosin / Q000378:metabolism*; D005782:Gels / Q000737:chemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000737:chemistry / Q000201:enzymology; D007700:Kinetics; D008894:Milk Proteins / Q000737:chemistry / Q000378:metabolism*; D012212:Rheology; D013552:Swine / Q000378:metabolism*; D013556:Swine, Miniature
D000596:Amino Acids; D005782:Gels; D008894:Milk Proteins; C025350:rennet; D012085:Chymosin
Acid gelation; Amino acid absorption; Bioavailability; Food structure; Gastrointestinal hormones; In vivo; Milk proteins; Rennet gelation