Single-molecule dynamics and mechanisms of metalloregulators and metallochaperones.
Understanding how cells regulate and transport metal ions is an important goal in the field of bioinorganic chemistry, a frontier research area that resides at the interface of chemistry and biology. This Current Topic reviews recent advances from the authors' group in using single-molecule fluorescence imaging techniques to identify the mechanisms of metal homeostatic proteins, including metalloregulators and metallochaperones. It emphasizes the novel mechanistic insights into how dynamic protein-DNA and protein-protein interactions offer efficient pathways via which MerR-family metalloregulators and copper chaperones can fulfill their functions. This work also summarizes other related single-molecule studies of bioinorganic systems and provides an outlook toward single-molecule imaging of metalloprotein functions in living cells.
Department of Chemistry and Chemical Biology, Cornell University , Ithaca, New York 14853, United States.
Peng
P
Chen
Aaron M
AM
Keller
Chandra P
CP
Joshi
Danya J
DJ
Martell
Nesha May
NM
Andoy
Jaime J
JJ
Benítez
Tai-Yen
TY
Chen
Ace George
AG
Santiago
Feng
F
Yang
D000818:Animals; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D056511:Metallochaperones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008670:Metals / Q000378:metabolism*
D004268:DNA-Binding Proteins; D056511:Metallochaperones; D008670:Metals