Dietary CLA-induced lipolysis is delayed in soy oil-fed mice compared to coconut oil-fed mice.
Conjugated linoleic acid (CLA) has been shown to cause a reduction in obesity in several species. CLA-induced body fat loss is enhanced when mice are fed coconut oil (CO) and involves increased lipolysis. The objective of this paper was to determine if the CLA-induced lipolysis in mice fed with different oil sources was time-dependent. Mice were fed 7 % soybean oil (SO) or CO diets for 6 week and then supplemented with 0 or 0.5 % CLA for 3, 7, 10 or 14 days. Body fat and ex-vivo lipolysis was determined. Body fat was reduced by CO on day 7 (P < 0.01) and in both CO and SO-fed mice (P < 0.05) in response to CLA on d14. Lipolysis was increased by CLA in CO-fed mice (P < 0.01) but not in SO-fed mice on day 7 and 10, but on day 14 CLA increased lipolysis in both CO- and SO-fed mice (P < 0.001). Expression and activation level of proteins involved in lipolysis and lipogenesis was determined by western blotting and real-time PCR, respectively. No significant differences were detected in protein expression. CO-fed mice had greater fatty acid synthase and stearyl CoA desaturase 1 mRNA expression and less acetyl CoA carboxylase mRNA expression (P < 0.01). Sterol regulatory binding protein 1c was decreased by CLA in CO-fed mice and increased in SO-fed mice (P < 0.05). Malic enzyme expression was increased by CLA (P < 0.001) and CO (P < 0.01). Therefore, CLA-induced lipolysis occurs more rapidly in CO vs SO-fed mice and lipogenesis is decreased in CO-fed mice with CLA supplementation.
Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, 26506-6108, USA.
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Angius
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D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism; D000284:Administration, Oral; D000818:Animals; D019440:Anti-Obesity Agents / Q000008:administration & dosage*; D001823:Body Composition / Q000187:drug effects; D000074263:Coconut Oil; D004032:Diet; D019587:Dietary Supplements; D064429:Fatty Acid Synthases / Q000235:genetics / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood; D015870:Gene Expression; D050152:Intra-Abdominal Fat / Q000187:drug effects / Q000378:metabolism; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage*; D050155:Lipogenesis; D008066:Lipolysis / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010938:Plant Oils / Q000008:administration & dosage*; D013024:Soybean Oil / Q000008:administration & dosage*; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism
D019440:Anti-Obesity Agents; D005230:Fatty Acids, Nonesterified; D044243:Linoleic Acids, Conjugated; D010938:Plant Oils; D013024:Soybean Oil; D013230:Stearoyl-CoA Desaturase; D064429:Fatty Acid Synthases; D000103:Acetyl-CoA Carboxylase; D000074263:Coconut Oil