Heat stress promotes skeletal muscle regeneration after crush injury in rats.
Influences of heat stress on skeletal muscle regeneration were examined in experimental rats. After crush injury to the Extensor digitorum longus muscle (EDL) of the left hindlimb, animals were randomly divided into non-heat and heat groups. In the latter, packs filled with hot water (42°C) were percutaneously applied to the injured EDL muscle for 20min to the front of the lower leg, soon after the injury. During the early stages of muscle regeneration, due to the heat stress, secondary degeneration at the injured site progressed faster, and migration of macrophages, proliferation and differentiation of satellite cells were facilitated. At 14 and 28 days after the injury, the ratio of regenerating muscle fibers exhibiting central nuclei in the heat treated group was significantly lower than that in the non-heat group, and cross sectional area in the heat group was evidently larger than that in the non-heat group. Moreover, in the heat group, the ratio of collagen fiber area at 14 and 28 days after the injury was smaller than in the non-heat group. Together, these findings suggest that acceleration of degeneration processes by heat stress soon after injury is likely to promote skeletal muscle regeneration and inhibit collagen deposition.
Department of Physical Therapy, Faculty of Health Sciences, Kobe University School of Medicine, 7-10-2 Tomogaoka, Sumaku, Kobe, 654-0142, Japan.
Kousuke
K
Takeuchi
Department of Physical Therapy, Faculty of Health Sciences, Kobe University School of Medicine, 7-10-2 Tomogaoka, Sumaku, Kobe, 654-0142, Japan; Department of Rehabilitation Sciences, Kobe University Graduate School of Health Sciences, 7-10-2 Tomogaoka, Sumaku, Kobe, 654-0142, Japan.
Takuya
T
Hatade
Department of Physical Therapy, Faculty of Health Sciences, Kobe University School of Medicine, 7-10-2 Tomogaoka, Sumaku, Kobe, 654-0142, Japan.
Soushi
S
Wakamiya
Department of Rehabilitation Sciences, Kobe University Graduate School of Health Sciences, 7-10-2 Tomogaoka, Sumaku, Kobe, 654-0142, Japan.
Naoto
N
Fujita
Department of Rehabilitation Sciences, Kobe University Graduate School of Health Sciences, 7-10-2 Tomogaoka, Sumaku, Kobe, 654-0142, Japan. Electronic address: arakawa@people.kobe-u.ac.jp.
Takamitsu
T
Arakawa
Department of Rehabilitation Sciences, Kobe University Graduate School of Health Sciences, 7-10-2 Tomogaoka, Sumaku, Kobe, 654-0142, Japan.
Akinori
A
Miki
D000818:Animals; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D018482:Muscle, Skeletal / Q000293:injuries / Q000502:physiology*; D017208:Rats, Wistar; D012038:Regeneration; D013312:Stress, Physiological
Collagen; Degeneration; Hyperthermic; Macrophages; Muscle crush; Proteolysis; Rat