Operation of a benchtop bioreactor.
Fermentation systems are used to provide an optimal growth environment for many different types of cell cultures. The ability afforded by fermentors to carefully control temperature, pH, and dissolved oxygen concentrations in particular makes them essential to efficient large scale growth and expression of fermentation products. This video will briefly describe the advantages of the fermentor over the shake flask. It will also identify key components of a typical benchtop fermentation system and give basic instruction on setup of the vessel and calibration of its probes. The viewer will be familiarized with the sterilization process and shown how to inoculate the growth medium in the vessel with culture. Basic concepts of operation, sampling, and harvesting will also be demonstrated. Simple data analysis and system cleanup will also be discussed.
Center for Biotechnology Education, Krieger School of Arts and Sciences, Johns Hopkins University.
Kristina M
KM
Obom
Andrew
A
Magno
Patrick J
PJ
Cummings
D001419:Bacteria / Q000254:growth & development / Q000378:metabolism; D057005:Bioengineering / Q000295:instrumentation* / Q000379:methods*; D019149:Bioreactors / Q000382:microbiology*; D005285:Fermentation