Triose phosphate isomerase from the blood fluke Schistosoma mansoni: biochemical characterisation of a potential drug and vaccine target.
The glycolytic enzyme triose phosphate isomerase from Schistosoma mansoni is a potential target for drugs and vaccines. Molecular modelling of the enzyme predicted that a Ser-Ala-Asp motif which is believed to be a helminth-specific epitope is exposed. The enzyme is dimeric (as judged by gel filtration and cross-linking), resistant to proteolysis and highly stable to thermal denaturation (melting temperature of 82.0 °C). The steady-state kinetic parameters are high (Km for dihydroxyacetone phosphate is 0.51 mM; Km for glyceraldehyde 3-phosphate is 1.1 mM; kcat for dihydroxyacetone phosphate is 7800 s(-1) and kcat for glyceraldehyde 3-phosphate is 6.9s(-1)).
School of Biological Sciences, Queen's University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UK.
Veronika L
VL
Zinsser
Edward
E
Farnell
David W
DW
Dunne
David J
DJ
Timson
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D004099:Dihydroxyacetone Phosphate / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D005986:Glyceraldehyde 3-Phosphate / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D012550:Schistosoma mansoni / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism; D014305:Triose-Phosphate Isomerase / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism
D000947:Antigens, Helminth; D000939:Epitopes; D005986:Glyceraldehyde 3-Phosphate; D004099:Dihydroxyacetone Phosphate; D014305:Triose-Phosphate Isomerase
Bilharzia; Blood fluke; Glycolytic enzyme; Schistosomiasis; Vaccine target