Detection of Leishmania siamensis DNA in saliva by polymerase chain reaction.
Polymerase chain reaction was used to detect Leishmania siamensis DNA from clinical samples collected from six leishmaniasis patients during 2011-2012. The samples used in this study came from bone marrow, blood, buffy coat, saliva, urine, and tissue biopsy specimens. Saliva was a good source for L. siamensis DNA by polymerase chain reaction. L. siamensis DNA was also found in saliva of an asymptomatic case-patient. Levels of L. siamensis DNA in saliva decreased until being undetectable after treatment. These levels could be used as a marker to evaluate efficacy of the treatment. A larger study is needed to evaluate this method as a screening and survey tool to study the silent background of Leishmania infection among the at-risk population.
Medical Sciences Program, Department of Parasitology, Division of Dermatology, and Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand; Bumrungrad International Hospital, Bangkok, Thailand; Chiangrai Prachanukroh Hospital, Chiang Rai, Thailand; Excellence Center for Emerging Infectious Diseases, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand.
Atchara
A
Phumee
Kanyarat
K
Kraivichian
Sarunyou
S
Chusri
Nopadon
N
Noppakun
Asda
A
Vibhagool
Vivornpun
V
Sanprasert
Vich
V
Tampanya
Henry
H
Wilde
Padet
P
Siriyasatien
D000328:Adult; D000595:Amino Acid Sequence; D000981:Antiprotozoal Agents / Q000627:therapeutic use; D015415:Biomarkers / Q000378:metabolism; D016054:DNA, Protozoan / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007891:Leishmania / Q000235:genetics* / Q000302:isolation & purification; D007898:Leishmaniasis, Visceral / Q000175:diagnosis* / Q000188:drug therapy / Q000469:parasitology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012463:Saliva / Q000469:parasitology*
D000981:Antiprotozoal Agents; D015415:Biomarkers; D016054:DNA, Protozoan