[Effect of buqi tongluo jiedu recipe on HIC1 methylation level of pancreatic cancer model mice].
OBJECTIVE
To observe the effect of Buqi Tongluo Jiedu Recipe (BTJR) on HIC1 methylation of pancreatic cancer mouse model.


METHODS
Totally 30 nude mice were randomly divided into the normal group, the model group, and the treatment group, 10 in each group. The model was induced by cancer cell subcutaneous planting method. Mice in the treatment group were administered with BTJR (60 g crude drugs/kg, 3 mL/100 g) by gastrogavage. Equal volume of normal saline was given to those in the normal group and the model group by gastrogavage. All the intervention lasted for 14 successive days. The mice were sacrificed to death. Their tumor tissues were taken out. The HIC1 gene methylation level of each specimen was detected by nested methylation-specific polymerase chain reaction (N-MSP) method.


RESULTS
Compared with the model group, the HIC1 gene methylation level was lower in the treatment group (P < 0.01). Compared with the model, the HIC1 gene methylation level was even lower in the normal group (P < 0.01).


CONCLUSIONS
BTJR could reduce HIC1 gene methylation degree of model mice, thus playing a role of molecular biologic effect in treating pancreatic cancer.
The Postdoctoral Research Station of Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China. hubo760913@sina.com
Bo
B
Hu
Chun-You
CY
Wang
D000818:Animals; D045744:Cell Line, Tumor; D019175:DNA Methylation; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010190:Pancreatic Neoplasms / Q000188:drug therapy* / Q000235:genetics*; D024363:Transcription Initiation Site
D004365:Drugs, Chinese Herbal; C096870:Hic1 protein, mouse; D051741:Kruppel-Like Transcription Factors; C527174:tongluo