Experimental assessment of the neo-vascularisation of acellular dermal matrix in the wound bed pretreated with mesenchymal stem cell under subatmospheric pressure.
Neo-vascularisation of the acellular dermal matrix (ADM) is an essential procedure if a full-thickness wound is closed with ADM and skin is grafted over the ADM. In this study, we aimed to improve the neo-vascularisation of ADM by combining the effects of negative pressure wound therapy (NPWT) and mesenchymal stem cells (MSCs) on angiogenesis. In this study, 28 female Sprague-Dawley rats were used and divided into four groups. Full-thickness dorsal skin defects were created in 2 × 2 cm dimensions. The wounds were treated with only the ADM in group 1, the ADM and NPWT in group 2, the ADM and MSCs in group 3 and the ADM, NPWT and MSCs in group 4. By the ninth day of surgery, the excisional biopsy samples were histologically examined to identify the rates of ADM adherence to the recipient bed; the newly formed blood vessels which penetrate the ADM vertically and vascularisation were evaluated by immunohistochemical staining. The graft adherence rates were higher in group 4 than in the other groups statistically, p = 0.003. The numbers of cluster of differentiation 31 (CD31)-stained newly formed microvessels were higher in group 4 than in the other groups statistically, p < 0.05. All subjects in group 4 had the vertical vessels in normal calibration with open lumen vessels which penetrate the ADM. These findings suggest that MSC transplantation induces angiogenesis more efficiently than NPWT. The combination of the NPWT with MSC in this study has shown a synergistic effect on angiogenesis and has affected the neo-vascularisation of the ADM significantly.
Department of Plastic and Reconstructive Surgery, Gulhane Military Medical Academy, Ankara, Turkey. Electronic address: drismailsahin@yahoo.com.
Ismail
I
Sahin
Department of Plastic and Reconstructive Surgery, Gulhane Military Medical Academy, Ankara, Turkey.
Sinan
S
Ozturk
Department of Plastic and Reconstructive Surgery, Numune Egitim ve Arastirma Hastanesi, Ankara, Turkey.
Mustafa
M
Deveci
Department of Haematology, Bayindir Hospital, Ankara, Turkey.
Ali Ugur
AU
Ural
Department of Pathology, Gulhane Military Medical Academy, Ankara, Turkey.
Onder
O
Onguru
Department of Plastic and Reconstructive Surgery, Gulhane Military Medical Academy, Ankara, Turkey.
Selcuk
S
Isik
D062088:Acellular Dermis; D000818:Animals; D005260:Female; D045164:Mesenchymal Stem Cell Transplantation; D055806:Microvessels / Q000033:anatomy & histology* / Q000502:physiology; D054843:Negative-Pressure Wound Therapy; D018919:Neovascularization, Physiologic; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000098:blood supply* / Q000166:cytology / Q000293:injuries; D014945:Wound Healing
Acellular dermal matrix; Mesenchymal stem cells; Negative pressure wound therapy; Neo-vascularisation