Differential potential for envelope glycoprotein-mediated steric shielding of host cell surface proteins among filoviruses.
The viral envelope glycoprotein (GP) is thought to play important roles in the pathogenesis of filovirus infection. It is known that GP expressed on the cell surface forms a steric shield over host proteins such as major histocompatibility complex class I and integrin β1, which may result in the disorder of cell-to-cell contacts and/or inhibition of the immune response. However, it is not clarified whether this phenomenon contributes to the pathogenicity of filoviruses. In this study, we found that the steric shielding efficiency differed among filovirus strains and was correlated with the difference in their relative pathogenicities. While the highly glycosylated mucin-like region of GP was indispensable, the differential shielding efficiency did not necessarily depend on the primary structure of the mucin-like region, suggesting the importance of the overall properties (e.g., flexibility and stability) of the GP molecule for efficient shielding of host proteins.
Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Kita-20, Nishi-10, Kita-ku, Sapporo 001-0020, Japan.
Osamu
O
Noyori
Keita
K
Matsuno
Masahiro
M
Kajihara
Eri
E
Nakayama
Manabu
M
Igarashi
Makoto
M
Kuroda
Norikazu
N
Isoda
Reiko
R
Yoshida
Ayato
A
Takada
D002460:Cell Line; D004252:DNA Mutational Analysis; D016563:Filoviridae / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000378:metabolism*; D014774:Virulence
D008565:Membrane Proteins; D014759:Viral Envelope Proteins
Ebola virus; Glycoprotein; Integrin; MHC; Marburg virus; Pathogenicity; Steric shielding