Highly efficient synthesis of (R)-3-quinuclidinol in a space-time yield of 916 g L(-1) d(-1) using a new bacterial reductase ArQR.
A new keto reductase (ArQR), identified from Agrobacterium radiobacter ECU2556, can efficiently reduce 3-quinuclidinone in excellent enantioselectivity and high space-time yield for the synthesis of (R)-3-quinuclidinol, a chiral building block of many antimuscarinic agents. This is the first time that a high yield of (R)-3-quinuclidinol up to 916 g L(-1) d(-1) using a bioreduction approach is reported.
Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , 130 Meilong Road, Shanghai 200237, People' s Republic of China.
Wen-Xia
WX
Zhang
Guo-Chao
GC
Xu
Lei
L
Huang
Jiang
J
Pan
Hui-Lei
HL
Yu
Jian-He
JH
Xu
D063988:3-Hydroxyacyl-CoA Dehydrogenase / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry*; D011812:Quinuclidines / Q000138:chemical synthesis* / Q000737:chemistry*; D013237:Stereoisomerism
D011812:Quinuclidines; C090998:3-quinuclidinone; C474540:3-quinuclidinol; D063988:3-Hydroxyacyl-CoA Dehydrogenase