Xyloglucan-based diblock co-oligomer: synthesis, self-assembly and steric stabilization of proteins.
We propose a novel plant-based amphiphilic diblock co-oligomers (BCO) surfactant containing only carbohydrate segments and examine its potential as a biosourced stabilizer. The synthesis of an amphiphilic xyloglucan-based BCO, composed of a hydrophilic xyloglucan oligosaccharide (XGO) block "clicked" to a hydrophobic peracetylated XGO is described. Dynamic light scattering experiments correlated with transmission electron microscopy observations showed that this new class of amphiphilic BCO self-assembles in water to form spherical micelles with a hydrodynamic diameter of 22 nm. Preliminary studies indicate that the XGO-based BCO sterically stabilizes gliadin and zein nanoparticle suspensions. The stabilization results were compared to those using pluronic F-68, a commercial surfactant. For gliadin nanoparticles, both surfactants result in essentially the same morphology and polydispersity. However, for the zein nanoparticles, the XGO-based BCO stabilizer gave lower polydispersity.
Centre de Recherches sur les Macromolécules Végétales (CERMAV-CNRS), Université Joseph Fourier (UJF), Institut de Chimie Moléculaire de Grenoble (ICMG-FR 2607 CNRS), PolyNat Carnot Institute, Arcane LabEx, BP 53, F-38041 Grenoble Cedex 9, France; Department of Chemistry, Universidade Federal de Santa Catarina, 88040-900, Florianópolis, Brazil.
Cony
C
Gauche
Valdir
V
Soldi
Sébastien
S
Fort
Redouane
R
Borsali
Sami
S
Halila
D002240:Carbohydrate Sequence; D005903:Gliadin / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008027:Light; D008823:Micelles; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D020442:Poloxamer / Q000737:chemistry; D058105:Polymerization; D055550:Protein Stability; D012542:Scattering, Radiation; D013535:Suspensions; D014867:Water; D014990:Xylans / Q000737:chemistry*; D015028:Zein / Q000737:chemistry*
D005936:Glucans; D008823:Micelles; D013535:Suspensions; D014990:Xylans; D014867:Water; D020442:Poloxamer; C029353:xyloglucan; D005903:Gliadin; D015028:Zein
Amphiphilic block co-oligomer; Click reaction; Micelles; Protein stabilization; Self-assembly; Xyloglucan oligosaccharides