Identification of immune response-related genes in the Chinese oak silkworm, Antheraea pernyi by suppression subtractive hybridization.
Insects possess an innate immune system that responds to invading microorganisms. In this study, a subtractive cDNA library was constructed to screen for immune response-related genes in the fat bodies of Antheraea pernyi (Lepidoptera: Saturniidae) pupa challenged with Escherichia coli. Four hundred putative EST clones were identified by suppression subtractive hybridization (SSH), including 50 immune response-related genes, three cytoskeleton genes, eight cell cycle and apoptosis genes, five respiration and energy metabolism genes, five transport genes, 40 metabolism genes, ten stress response genes, four transcription and translation regulation genes and 77 unknown genes. To verify the reliability of the SSH data, the transcription of a set of randomly selected immune response-related genes were confirmed by semi-quantitative reverse transcription-PCR (RT-PCR) and real-time quantitative reverse transcription-PCR (qRT-PCR). These identified immune response-related genes provide insight into understanding the innate immunity in A. pernyi.
College of Life Sciences, Anhui Agricultural University, Hefei 230036, PR China.
Qiu-Ning
QN
Liu
Bao-Jian
BJ
Zhu
Lei
L
Wang
Guo-Qing
GQ
Wei
Li-Shang
LS
Dai
Kun-Zhang
KZ
Lin
Yu
Y
Sun
Jian-Feng
JF
Qiu
Wei-Wei
WW
Fu
Chao-Liang
CL
Liu
D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000276:immunology* / Q000382:microbiology; D004926:Escherichia coli / Q000276:immunology; D005216:Fat Body / Q000378:metabolism; D015723:Gene Library; D017344:Genes, Insect; D007113:Immunity, Innate / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment
Antheraea pernyi; Escherichia coli; Immune response-related gene; Suppression subtractive hybridization