Oxidation of methane by Methylomicrobium album and Methylocystis sp. in the presence of H2S and NH 3.
Oxidation of methane by methanotrophs, Methylomicrobium album and Methylocystis sp., was measured at several initial concentrations of H2S and NH3 in the headspace of stoppered flasks, at the same initial concentration of methane as sole carbon and energy source: 15 % (v/v). No effect was observed at 0.01 % (v/v) H2S and 0.025 % (v/v) NH3 in gas phase but over 0.05 and 0.025 % (v/v), respectively, they inhibited the oxidation of methane. The effect of H2S was stronger in Methylocystis sp. and both microorganisms were similarly affected by NH3. Depending on their concentrations in gas phase, H2S and NH3 can thus affect the rate of oxidation of methane and biomass growth of both methanotrophs.
School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2147, Valparaíso, Chile.
Manuel
M
Cáceres
Juan C
JC
Gentina
Germán
G
Aroca
D000641:Ammonia / Q000378:metabolism / Q000494:pharmacology*; D002245:Carbon Dioxide; D006862:Hydrogen Sulfide / Q000378:metabolism / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008697:Methane / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D008772:Methylococcaceae / Q000378:metabolism*; D042083:Methylocystaceae / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects
D002245:Carbon Dioxide; D000641:Ammonia; D008697:Methane; D006862:Hydrogen Sulfide