[Formation of pyrrole adducts in 2,5-hexanedione-containing human serum cultured in vitro].
OBJECTIVE
To investigate the relationship between formation of pyrrole adducts and concentration of 2, 5-hexanedione (2, 5-HD) and to provide an experimental basis for the study on toxicity of n-hexane.


METHODS
Serum samples were collected from normal persons and were then filtered and sterilized. They were mixed with 2,5-HD to obtain sera with final 2, 5-HD concentrations of 10, 25, 50, 100, and 200 mg/L, and blank serum was also prepared. The sera were cultured at 37°C and taken at different time points. Colorimetry was used to quantify the pyrrole adducts formed in sera, and gas chromatography was used to measure the remaining 2, 5-HD levels in sera.


RESULTS
The content of pyrrole adducts increased as the culture proceeded and was dependent on the dose of 2, 5-HD; at the end of the experiment, the content of pyrrole adducts differed significantly across all concentration groups (P < 0.5). The concentrations of 2,5-HD decreased as the culture proceeded; at the end of the experiment, the concentrations of 2, 5-HD, from the highest to the lowest, decreased by 29%, 55%, 22%, 44%, and 40%, respectively. The decrease in 2, 5-HD had a positive correlation with the increase in pyrrole adducts, and the correlation coefficients for 200∼10 mg/L 2, 5-HD were 0.865, 0.697, 0.835, 0.823, and 0.814, respectively.


CONCLUSIONS
The content of formed pyrrole adducts increases as the concentration of 2,5-HD rises; there is a positive correlation between the decrease in 2, 5-HD and the increase in pyrrole adducts in human serum.
Institute of Toxicology, School of public health, Shandong University, Jinan, Shandong Province 250012, China.
Ming-xing
MX
Zhu
Hong-yin
HY
Yin
Ke-qin
KQ
Xie
D006588:Hexanones / Q000737:chemistry*; D006801:Humans; D010084:Oxidation-Reduction; D011758:Pyrroles / Q000737:chemistry*; D044967:Serum / Q000737:chemistry*
D006588:Hexanones; D011758:Pyrroles; C011269:2,5-hexanedione