Enhancement of herboxidiene production in Streptomyces chromofuscus ATCC 49982.
Structurally, herboxidiene contains the tetrahydropyran acetic acid moiety and a side chain including a conjugated diene, and has been isolated from Streptomyces chromofuscus ATCC 49982. Its production was significantly elevated nearly 13.5-fold (0.74 g/l) in a medium supplemented with glycerol (medium No. 6A6), and was more efficacious (1.08 g/l; 19.8-fold) in fed-batch fermentation at 36 h in medium No. 6A6, from Streptomyces chromofuscus. For further enhancement, regulatory genes metK1-sp and afsR-sp from Streptomyces peucetius were overexpressed using an expression vector, pIBR25, and similarly ACCase from Streptomyces coelicolor and two genes, metK1-sp and afsR-sp, were also overexpressed using an integration vector, pSET152, under the control of the strong ermE* promoter in Streptomyces chromofuscus. Only the recombinant strains S. chromofuscus SIBR, S. chromofuscus GIBR, and S. chromofuscus AFS produced more herboxidiene than the parental strain in optimized medium No. 6A6 with an increment of 1.32-fold (0.976 g/l), 3.85-fold (2.849 g/l), and 1.7-fold(1.258 g/l) respectively.
Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, SunMoon University, Asansi 336-708, Republic of Korea.
Amit Kumar
AK
Jha
Janardan
J
Lamichhane
Jae Kyung
JK
Sohng
D003470:Culture Media / Q000737:chemistry; D005233:Fatty Alcohols / Q000378:metabolism*; D005285:Fermentation; D015870:Gene Expression; D005990:Glycerol / Q000378:metabolism; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways / Q000235:genetics; D011714:Pyrans / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*
D003470:Culture Media; D005233:Fatty Alcohols; D011714:Pyrans; D011994:Recombinant Proteins; C076087:herboxidiene; D005990:Glycerol