Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline.
Conophylline (CNP) has various biological activities, such as insulin production. A recent study identified ADP-ribosylation factor-like 6-interacting protein 1 (ARL6ip1) as a direct target protein of CNP. In this study, we revealed that ARL6ip1 is a three-spanning transmembrane protein and determined the CNP-binding domain of ARL6ip1 by deletion mutation analysis of ARL6ip1 with biotinyl-amino-CNP. These results suggest that CNP is expected to be useful for future investigation of ARL6ip1 function in cells. Because of the anti-apoptotic function of ARL6ip1, CNP may be an effective therapeutic drug and/or a novel chemosensitizer for human cancers and other diseases.
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan.
Masahiro
M
Kuroda
Shintaro
S
Funasaki
Tsuyoshi
T
Saitoh
Yukiko
Y
Sasazawa
Shigeru
S
Nishiyama
Kazuo
K
Umezawa
Siro
S
Simizu
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry*; D000595:Amino Acid Sequence; D001665:Binding Sites; D057809:HEK293 Cells; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010936:Plant Extracts / Q000737:chemistry*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D014748:Vinca Alkaloids / Q000737:chemistry*
C488848:ARL6IP1 protein, human; D048868:Adaptor Proteins, Signal Transducing; D008565:Membrane Proteins; D010936:Plant Extracts; D014748:Vinca Alkaloids; C099167:conophylline
ADP-ribosylation factor-like 6-interacting protein 1; ARL6ip1; BCNP; CNP; Conophylline; ER; Gaussia luciferase; Gluc; Redox-sensitive luciferase assay; TM; Topology; biotinyl-aminoconophylline; conophylline; endoplasmic reticulum; transmembrane