Genomic sequence analysis of Helicoverpa armigera nucleopolyhedrovirus isolated from Australia.
The complete genomic sequence of Helicoverpa armigera nucleopolyhedrovirus from Australia, HearNPV-Au, was determined and analyzed. The HearNPV-Au genome was 130,992 bp in size with a G+C content of 39 mol% and contained 134 predicted open reading frames (ORFs) consisting of more than 150 nucleotides. HearNPV-Au shared 94 ORFs with AcMNPV, HearSNPV-G4 and SeMNPV, and was most closely related to HearSNPV-G4. The nucleotide sequence identity between HearNPV-Au and HearSNPV-G4 genome was 99%. The major differences were found in homologous regions (hrs) and baculovirus repeat ORFs (bro) genes. Five hrs and two bro genes were identified in the HearNPV-Au genome. All of the 134 ORFs identified in HearNPV-Au were also found in HearSNPV-G4, except the homologue of ORF59 (bro) in HearSNPV-G4. The sequence data strongly suggested that HearNPV-Au and HearSNPV-G4 belong to the same virus species.
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China, zhanghuan@ioz.ac.cn.
Huan
H
Zhang
Qing
Q
Yang
Qi-Lian
QL
Qin
Wei
W
Zhu
Zhi-Fang
ZF
Zhang
Yi-Nü
YN
Li
Ning
N
Zhang
Ji-Hong
JH
Zhang
D000818:Animals; D001315:Australia; D001482:Base Composition; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D005814:Genes, Viral; D016679:Genome, Viral; D007915:Lepidoptera / Q000821:virology; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D026801:Synteny
D004279:DNA, Viral