Antioxidant and cytotoxic effect of biologically synthesized selenium nanoparticles in comparison to selenium dioxide.
The present study was designed to evaluate antioxidant and cytotoxic effect of selenium nanoparticles (Se NPs) biosynthesized by a newly isolated marine bacterial strain Bacillus sp. MSh-1. An organic-aqueous partitioning system was applied for purification of the biogenic Se NPs and the purified Se NPs were then investigated for antioxidant activity using DPPH scavenging activity and reducing power assay. Cytotoxic effect of the biogenic Se NPs and selenium dioxide (SeO2) on MCF-7 cell line was assesed by MTT assay. Tranmission electron micrograph (TEM) of the purified Se NPs showed individual and spherical nanostructure in size range of about 80-220nm. The obtained results showed that, at the same concentration of 200μg/mL, Se NPs and SeO2 represented scavenging activity of 23.1±3.4% and 13.2±3.1%, respectively. However, the data obtained from reducing power assay revealed higher electron-donating activity of SeO2 compared to Se NPs. Higher IC50 of the Se NPs (41.5±0.9μg/mL) compared to SeO2 (6.7±0.8μg/mL) confirmed lower cytotoxicity of the biogenic Se NPs on MCF-7 cell line.
Pharmaceutics Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Hamid
H
Forootanfar
Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Mahboubeh
M
Adeli-Sardou
The Student Research Committee, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
Maryam
M
Nikkhoo
Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Mitra
M
Mehrabani
Pharmaceutics Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Bagher
B
Amir-Heidari
Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Ahmad Reza
AR
Shahverdi
Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran. Electronic address: shakiba@kmu.ac.ir.
Mojtaba
M
Shakibaie
D000975:Antioxidants / Q000378:metabolism*; D001713:Biphenyl Compounds; D006801:Humans; D061986:MCF-7 Cells; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D010851:Picrates; D012643:Selenium / Q000737:chemistry / Q000378:metabolism*
D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl; D012643:Selenium
Antioxidant; Biogenic selenium nanoparticles; MCF-7 cell line; Selenium dioxide