Secreted phospholipase A2 is increased in meconium-stained amniotic fluid of term gestations: potential implications for the genesis of meconium aspiration syndrome.
BACKGROUND
Meconium-stained amniotic fluid (MSAF) represents the passage of fetal colonic content into the amniotic cavity. Meconium aspiration syndrome (MAS) is a complication that occurs in a subset of infants with MSAF. Secreted phospholipase A2 (sPLA2) is detected in meconium and is implicated in the development of MAS. The purpose of this study was to determine if sPLA2 concentrations are increased in the amniotic fluid of women in spontaneous labor at term with MSAF.


METHODS
This was a cross-sectional study of patients in spontaneous term labor who underwent amniocentesis (n = 101). The patients were divided into two study groups: (1) MSAF (n = 61) and (2) clear fluid (n = 40). The presence of bacteria and endotoxin as well as interleukin-6 (IL-6) and sPLA2 concentrations in the amniotic fluid were determined. Statistical analyses were performed to test for normality and bivariate analysis. The Spearman correlation coefficient was used to study the relationship between sPLA2 and IL-6 concentrations in the amniotic fluid.


RESULTS
Patients with MSAF have a higher median sPLA2 concentration (ng/mL) in amniotic fluid than those with clear fluid [1.7 (0.98-2.89) versus 0.3 (0-0.6), p < 0.001]. Among patients with MSAF, those with either microbial invasion of the amniotic cavity (MIAC, defined as presence of bacteria in the amniotic cavity), or bacterial endotoxin had a significantly higher median sPLA2 concentration (ng/mL) in amniotic fluid than those without MIAC or endotoxin [2.4 (1.7-6.0) versus 1.7 (1.3-2.5), p < 0.05]. There was a positive correlation between sPLA2 and IL-6 concentrations in the amniotic fluid (Spearman Rho = 0.3, p < 0.05).


CONCLUSIONS
MSAF that contains bacteria or endotoxin has a higher concentration of sPLA2, and this may contribute to induce lung inflammation when meconium is aspirated before birth.
Perinatology Research Branch, NICHD/NIH/DHHS , Bethesda, MD and Detroit, MI , USA .
Roberto
R
Romero
Bo Hyun
BH
Yoon
Piya
P
Chaemsaithong
Josef
J
Cortez
Chan-Wook
CW
Park
Rogelio
R
Gonzalez
Ernesto
E
Behnke
Sonia S
SS
Hassan
Francesca
F
Gotsch
Lami
L
Yeo
Tinnakorn
T
Chaiworapongsa
D000649:Amniocentesis; D000653:Amniotic Fluid / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology; D015415:Biomarkers / Q000378:metabolism; D003430:Cross-Sectional Studies; D004731:Endotoxins / Q000032:analysis; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D015850:Interleukin-6 / Q000378:metabolism; D008471:Meconium Aspiration Syndrome / Q000209:etiology* / Q000378:metabolism; D054497:Phospholipases A2, Secretory / Q000378:metabolism*; D011247:Pregnancy; D047929:Term Birth
D015415:Biomarkers; D004731:Endotoxins; D015850:Interleukin-6; D054497:Phospholipases A2, Secretory
Acute phase protein reactant; interleukin-6; intra-amniotic inflammation/infection; prostaglandins; sPLA2