Biodiesel production from Jatropha oil catalyzed by immobilized Burkholderia cepacia lipase on modified attapulgite.
Lipase from Burkholderia cepacia was immobilized on modified attapulgite by cross-linking reaction for biodiesel production with jatropha oil as feedstock. Effects of various factors on biodiesel production were studied by single-factor experiment. Results indicated that the best conditions for biodiesel preparation were: 10 g jatropha oil, 2.4 g methanol (molar ratio of oil to methanol is 1:6.6) being added at 3h intervals, 7 wt% water, 10 wt% immobilized lipase, temperature 35°C, and time 24h. Under these conditions, the maximum biodiesel yield reached 94%. The immobilized lipase retained 95% of its relative activity during the ten repeated batch reactions. The half-life time of the immobilized lipase is 731 h. Kinetics was studied and the Vmax of the immobilized lipases were 6.823 mmol L(-1). This immobilized lipase catalyzed process has potential industrial use for biodiesel production to replace chemical-catalyzed method.
Huaiyin Institute of Technology, School of Life Science and Chemical Engineering, Huaian 223001 China; Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian 223001, China; Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaian 223300, China.
Qinghong
Q
You
Xiulian
X
Yin
Yuping
Y
Zhao
Yan
Y
Zhang
D055162:Biocatalysis / Q000187:drug effects*; D056804:Biofuels / Q000032:analysis*; D016956:Burkholderia cepacia / Q000187:drug effects / Q000201:enzymology*; D004795:Enzyme Stability / Q000187:drug effects; D004800:Enzymes, Immobilized / Q000378:metabolism; D031286:Jatropha / Q000737:chemistry*; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D017616:Magnesium Compounds / Q000494:pharmacology*; D000432:Methanol / Q000494:pharmacology; D010938:Plant Oils / Q000737:chemistry*; D059027:Recycling; D017655:Silicon Compounds / Q000494:pharmacology*; D013696:Temperature; D013997:Time Factors; D014867:Water
D056804:Biofuels; D004800:Enzymes, Immobilized; D017616:Magnesium Compounds; D010938:Plant Oils; D017655:Silicon Compounds; D014867:Water; D008049:Lipase; C026325:attapulgite; D000432:Methanol
Biodiesel production; Burkholderia cepacia; Immobilized lipase; Modified attapulgite