DNA detection using origami paper analytical devices.
We demonstrate the hybridization-induced fluorescence detection of DNA on an origami-based paper analytical device (oPAD). The paper substrate was patterned by wax printing and controlled heating to construct hydrophilic channels and hydrophobic barriers in a three-dimensional fashion. A competitive assay was developed where the analyte, a single-stranded DNA (ssDNA), and a quencher-labeled ssDNA competed for hybridization with a fluorophore-labeled ssDNA probe. Upon hybridization of the analyte with the fluorophore-labeled ssDNA, a linear response of fluorescence vs analyte concentration was observed with an extrapolated limit of detection <5 nM and a sensitivity relative standard deviation as low as 3%. The oPAD setup was also tested against OR/AND logic gates, proving to be successful in both detection systems.
Department of Chemistry and Biochemistry, Center for Nano- and Molecular Science and Technology, The University of Texas at Austin, 105 E. 24th St., Stop A5300, Austin, TX, 78712-1224, USA.
Karen
K
Scida
Department of Chemistry and Biochemistry, Center for Nano- and Molecular Science and Technology, The University of Texas at Austin, 105 E. 24th St., Stop A5300, Austin, TX, 78712-1224, USA.
Bingling
B
Li
Department of Chemistry and Biochemistry, Center for Nano- and Molecular Science and Technology, The University of Texas at Austin, 105 E. 24th St., Stop A5300, Austin, TX, 78712-1224, USA.
Andrew D
AD
Ellington
Department of Chemistry and Biochemistry, Center for Nano- and Molecular Science and Technology, The University of Texas at Austin, 105 E. 24th St., Stop A5300, Austin, TX, 78712-1224, USA.
Richard M
RM
Crooks
D001483:Base Sequence; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D039301:Computers, Molecular; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry; D008128:Logic; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010209:Paper
D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes