Confocal microscopy of cardiac myocytes.
Detailed methods are provided for the preparation and confocal imaging of cardiac myocyte development and differentiation. Examples include protocols for the analysis of cultured myocytes as well as vibratome sections of hearts from embryonic and adult tissue. Techniques include routine labeling of F-actin with phalloidin as well as multiple labeling protocols for colocalization studies and cell volume analysis.
Department of Cell and Developmental Biology and Anatomy, School of Medicine, University of South Carolina, Columbia, SC, USA.
Robert L
RL
Price
Stephen T
ST
Haley
Tara
T
Bullard
Jeffrey
J
Davis
Thomas K
TK
Borg
Louis
L
Terracio
D000199:Actins / Q000648:ultrastructure; D000328:Adult; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D006801:Humans; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D032383:Myocytes, Cardiac / Q000648:ultrastructure*
D000199:Actins