Live confocal analysis of mutant- and drug-treated Drosophila embryos.
The model organism Drosophila melanogaster is particularly well suited for live image analysis. The availability of GFP transgenic flies and a wide array of fluorescent probes, in conjunction with laser scanning confocal microscopy, allow us to image multiple aspects of the cell cycle simultaneously. Confocal microscopy provides the sensitivity and resolution to observe the dynamics of specific cellular events in real time. For example, GFP-histone and rhodamine-labeled tubulin enable one to follow specific nuclear and cytoskeletal events including nuclear envelope formation, nuclear envelope breakdown, spindle formation, centrosome duplication, separation and migration, chromosomes condensation, and segregation. This analysis permits a detailed morphological and temporal description of nuclear and cytoskeletal events in normal or drug-injected embryos.
Molecular and Cellular Biology, University of California, Santa Cruz, CA, USA.
Barbara
B
Fasulo
William
W
Sullivan
D000818:Animals; D030801:Animals, Genetically Modified; D004330:Drosophila / Q000187:drug effects / Q000196:embryology*; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000648:ultrastructure*; D049452:Green Fluorescent Proteins; D008938:Mitosis / Q000187:drug effects; D009154:Mutation; D009685:Nuclear Envelope / Q000187:drug effects / Q000648:ultrastructure; D012235:Rhodamines; D013194:Staining and Labeling; D014404:Tubulin / Q000378:metabolism
D012235:Rhodamines; D014404:Tubulin; D049452:Green Fluorescent Proteins