Altered red cell and platelet adhesion in hemolytic diseases: Hereditary spherocytosis, paroxysmal nocturnal hemoglobinuria and sickle cell disease.
OBJECTIVE
Intravascular hemolysis may have important pathophysiological consequences, such as the induction of cellular adhesion and vasculopathy. We compared the adhesive properties of red cells (RBC) and platelets in hereditary spherocytosis (HS), paroxysmal nocturnal hemoglobinuria (PNH) and sickle cell disease (SCD) patients.


METHODS
The adhesion of RBC and platelets, from patients and healthy subjects, was determined using static adhesion assays. RBC surface markers were characterized by flow cytometry and lactate dehydrogenase (LDH), plasma hemoglobin (pHb) and TNF-α were assayed in serum/plasma samples.


RESULTS
pHb levels were elevated in all three hemolytic diseases, indicating the incidence of intravascular hemolysis. RBC adhesion and TNF-α were augmented in HS and SCD, but not in PNH. Reticulocyte counts were raised in the three diseases, but were higher in HS and SCD than in PNH; high expressions of CD71, CD36 and CD49d were observed on SCD RBC, while CD71 alone was increased on HS and PNH RBC. Splenectomy was associated with reversals of increased pHb, RBC adhesion, reticulocytes, RBC marker expression and inflammation in HS. In contrast, platelet adhesion was elevated in SCD and PNH, but not HS. Platelet adhesion correlated significantly with serum LDH, but not pHb, in the hemolytic disease cohort; interestingly, LDH did not correlate with reticulocytes or pHb levels.


CONCLUSIONS
Results indicate that extravascular, rather than intravascular, hemolysis (and ensuing RBC production) may contribute to elevations in RBC adhesive properties in HS and SCD, while mechanisms peculiar to each disease may augment platelet adhesion in SCD and PNH.
INCT de Sangue, Haematology and Haemotherapy Centre, School of Medicine, University of Campinas - UNICAMP, Campinas, Sao Paulo, Brazil.
Tatiana M
TM
Sakamoto
Andreia A
AA
Canalli
Fabiola
F
Traina
Carla F
CF
Franco-Penteado
Sheley
S
Gambero
Sara T O
ST
Saad
Nicola
N
Conran
Fernando F
FF
Costa
D000293:Adolescent; D000328:Adult; D000368:Aged; D000755:Anemia, Sickle Cell / Q000097:blood*; D015703:Antigens, CD / Q000378:metabolism; D001792:Blood Platelets / Q000473:pathology; D018955:CD36 Antigens / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D004912:Erythrocytes / Q000473:pathology* / Q000502:physiology; D005260:Female; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood*; D006801:Humans; D039441:Integrin alpha4 / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D008875:Middle Aged; D010973:Platelet Adhesiveness; D000091402:Prohibitins; D011990:Receptors, Transferrin / Q000378:metabolism; D012016:Reference Values; D017701:Reticulocyte Count; D013103:Spherocytosis, Hereditary / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D055815:Young Adult
D015703:Antigens, CD; D018955:CD36 Antigens; C068126:CD71 antigen; C000717328:PHB protein, human; D000091402:Prohibitins; D011990:Receptors, Transferrin; D014409:Tumor Necrosis Factor-alpha; D039441:Integrin alpha4; D007770:L-Lactate Dehydrogenase
Adhesion; Intravascular hemolysis; Platelets; Red cells