Phenylboronic acid modified silver nanoparticles for colorimetric dynamic analysis of glucose.
The development of advanced nanostructures that allow dynamic quantification of glucose level can contribute to tight glucose control in diabetes management and other medical/biological fields. In this paper, we demonstrated that the assemblies of the 5-amino-2-fluorophenylboronic acid modified silver nanoparticles (FPBA-AgNPs) can be employed for highly modulating, sensitive, and selective colorimetric sensing of glucose over a physiologically important concentration range of 0-20mM at a physiological pH of 7.4. The glucose-modulated assembly of the FPBA-AgNPs occurred by the regulable formation of interparticle linkages via the bridged binding of 1,2-cis-diols and 5,6-cis-diols (for furanose form; or 4,6-cis-diols for pyranose form), respectively, of a glucose molecule to two FPBA-AgNPs. The detection limit was 89.0 μM. The mean error of glucose detection in a macro-bio-system, blood serum of adult, was smaller than 10%. Furthermore, we show that the glucose level variations associated with a model biological reaction process can be monitored by using the FPBA-AgNPs, whilst with the reaction mechanism remaining nearly unchanged.
State Key Laboratory for Physical Chemistry of Solid Surfaces, The Key Laboratory for Chemical Biology of Fujian Province, and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Ke
K
Cao
Xiaomei
X
Jiang
Suting
S
Yan
Laiying
L
Zhang
Weitai
W
Wu
D015374:Biosensing Techniques / Q000379:methods*; D001897:Boronic Acids / Q000737:chemistry; D003124:Colorimetry; D005947:Glucose / Q000302:isolation & purification*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*
D001897:Boronic Acids; D012834:Silver; D005947:Glucose; C010686:benzeneboronic acid
Colorimetric; Dynamic analysis; Glucose-sensing; Self-assembly; Silver nanoparticles