Evidence for the involvement of peripheral β-adrenoceptors in delayed liquid gastric emptying induced by dipyrone, 4-aminoantipyrine, and antipyrine in rats.
Dipyrone (Dp), 4-aminoantipyrine (AA), and antipyrine (At) delay liquid gastric emptying (GE) in rats. We evaluated adrenergic participation in this phenomenon in a study in male Wistar rats (250-300 g) pretreated subcutaneously with guanethidine (GUA), 100 mg·kg-1·day-1, or vehicle (V) for 2 days before experimental treatments. Other groups of animals were pretreated intravenously (iv) 15 min before treatment with V, prazosin (PRA; 1 mg/kg), yohimbine (YOH; 3 mg/kg), or propranolol (PRO; 4 mg/kg), or with intracerebroventricular (icv) administration of 25 µg PRO or V. The groups were treated iv with saline or with 240 µmol/kg Dp, AA, or At. GE was determined 10 min later by measuring the percentage of gastric retention (%GR) of saline labeled with phenol red 10 min after gavage. %GR (mean ± SE, n=8) indicated that GUA abolished the effect of Dp (GUA vs V=31.7 ± 1.6 vs 47.1 ± 2.3%) and of At (33.2 ± 2.3 vs 54.7 ± 3.6%) on GE and significantly reduced the effect of AA (48.1 ± 3.2 vs 67.2 ± 3.1%). PRA and YOH did not modify the effect of the drugs. %GR (mean ± SE, n=8) indicated that iv, but not icv, PRO abolished the effect of Dp (PRO vs V=29.1 ± 1.7 vs 46.9 ± 2.7%) and At (30.5 ± 1.7 vs 49 ± 3.2%) and significantly reduced the effect of AA (48.4 ± 2.6 vs 59.5 ± 3.1%). These data suggest activation of peripheral β-adrenoceptors in the delayed GE induced by phenylpyrazolone derivatives.
Universidade Estadual de Campinas, Núcleo de Medicina e Cirurgia Experimental, Faculdade de Ciências Médicas, CampinasSP, Brasil.
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Vinagre
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D018674:Adrenergic Antagonists / Q000008:administration & dosage*; D000675:Ampyrone / Q000008:administration & dosage*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage*; D000983:Antipyrine / Q000008:administration & dosage*; D004177:Dipyrone / Q000008:administration & dosage*; D005746:Gastric Emptying / Q000187:drug effects*; D057967:Infusions, Intraventricular; D008297:Male; D010637:Phenolsulfonphthalein; D011224:Prazosin / Q000008:administration & dosage; D011433:Propranolol / Q000008:administration & dosage; D017208:Rats, Wistar; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D015016:Yohimbine / Q000008:administration & dosage
D018674:Adrenergic Antagonists; D000894:Anti-Inflammatory Agents, Non-Steroidal; D011943:Receptors, Adrenergic, beta; D000675:Ampyrone; D015016:Yohimbine; D004177:Dipyrone; D011433:Propranolol; D010637:Phenolsulfonphthalein; D000983:Antipyrine; D011224:Prazosin