Human cytomegalovirus gB1 genotypes among children who live at the Phayathai Babies' home in Nonthaburi, Thailand.
We conducted a survey of human cytomegalovirus (HCMV) genotypes among 176 children aged 1 month to 5 years living at Phayathai Babies' Home in Nonthaburi Province, Thailand to determine the prevalence of HCMV glycoprotein B (gB) genotype. The study was conducted on urine samples using nested polymerase chain reaction and restriction fragment length polymorphism; the HCMV gB1 genotype was found in 89% of subjects, much higher than previous reports. Our results show a high proportion of HCMV gB1 infected children in this population.
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand. saowakon.pac@mahidol.ac.th
Saowakon
S
Paca-Uccaralertkun
Rungnapa
R
Hiatt
Rujee
R
Leecharoen
Peerapan
P
Tan-Ariya
Mathirut
M
Mungthin
Sureeporn
S
Pongphong
D002675:Child, Preschool; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000453:epidemiology* / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D019372:Orphanages / Q000706:statistics & numerical data*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D013785:Thailand; D014759:Viral Envelope Proteins / Q000235:genetics*
D004279:DNA, Viral; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus