Glucogalactan: a polysaccharide isolated from the cell-wall of Verticillium lecanii.
A water-soluble polysaccharide was extracted with alkali from the cell wall of Verticillium lecanii (also called Lecanicillium lecanii). After freezing and thawing, the water-soluble fraction was purified by gel filtration chromatography on Sepharose CL-6B and eluted as one peak by HPSEC/RID. Monosaccharide analysis showed galactose and glucose (1.1:1), with traces of mannose (<1%). The structural characteristics were determined by spectroscopic analysis, FT-IR and 1D and 2D (1)H and (13)C NMR, and methylation results. On the basis of the data obtained, the following structure of the polysaccharide (E3SIV fraction) was established: [Formula: see text] where n≈22 and m≈22.
Departamento de Física, Química e Biologia, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista, CEP 19060-900, Presidente Prudente, São Paulo, Brazil. Electronic address: corradi@fct.unesp.br.
Maria L
ML
Corradi da Silva
Diego T D
DT
Exposti
Ana F D
AF
Vasconcelos
Samara M A
SM
Alexandre
Joana L M
JL
Silveira
Diogo R B
DR
Ducatti
D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry*; D002850:Chromatography, Gel; D005685:Galactans / Q000737:chemistry* / Q000302:isolation & purification; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000737:chemistry; D008969:Molecular Sequence Data; D012685:Sepharose / Q000031:analogs & derivatives; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D020172:Verticillium / Q000737:chemistry*; D014867:Water
D005685:Galactans; D014867:Water; C047264:sepharose CL 6B; D012685:Sepharose; D005947:Glucose; D008358:Mannose; D005690:Galactose
Glucogalactan; Lecanicillium lecanii; NMR spectroscopy; Verticillium lecanii