Effect of a Thermoascus aurantiacus thermostable enzyme cocktail on wheat bread qualitiy.
Thermophilic fungus Thermoascus aurantiacus (CBMAI 756) on solid-state fermentation using corncob as a nutrient source produces an enzyme pool with the potential to be used in bread making. In this paper, the use of this enzyme cocktail as a wheat bread improver was reported. Both products released by flour arabinoxylan degradation and bread quality were investigated. The main product released through enzyme activity after prolonged incubation was xylose indicating the presence of xylanase; however, a small amount of xylobiose and arabinose also confirmed the presence of xylosidase and α-l-arabinofuranosidase, respectively. Enzyme mixture "in vitro" mainly attacked water-unextractable arabinoxylan contributing to beneficial effect in bread making. The use of an optimal enzyme concentration (35U xylanase/100g of flour) increased specific volume (22%), reduced crumb firmness (25%), and reduced amylopectin retrogradation (17%) during bread storage. In conclusion, the enzyme cocktail produced by T. aurantiacus CBMAI 756 can improve wheat bread quality.
Laboratory of Cereals, Roots, and Tubers, UNESP - São Paulo State University, Rua Cristovão Colombo, 2265 São José do Rio Preto, São Paulo CEP 15054-000, Brazil. Electronic address: denisefronteira@gmail.com.
D S
DS
Oliveira
J
J
Telis-Romero
R
R
Da-Silva
C M L
CM
Franco
D001939:Bread / Q000032:analysis*; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry; D005285:Fermentation; D005511:Food Handling; D005656:Fungal Proteins / Q000737:chemistry*; D055327:Thermoascus / Q000201:enzymology*; D014908:Triticum / Q000737:chemistry*
D005656:Fungal Proteins; D043364:Endo-1,4-beta Xylanases
Amylopectin retrogradation; Arabinoxylan; Bread staling; Crumb firmness; Thermoascus aurantiacus CBMAI 756; Xylanase