Effects of extraction methods on the antioxidant activities of polysaccharides obtained from Flammulina velutipes.
Four polysaccharides (CFP, UFP, MFP and EFP) were extracted from Flammulina velutipes using hot water, ultrasonic, microwave or enzymatic methods optimized by orthogonal test. Preliminary structural characterizations were conducted using physicochemical properties. Polysaccharides extracted by all four methods showed similar physicochemical characteristics and FT-IR spectra. However, SEM images of tissues of F. velutipes were significantly different. EFP demonstrated better antioxidant activities against hydroxyl radical as well as improved metal chelating activity. UFP showed higher DPPH scavenging activity, but CFP exhibited higher antioxidant activity in reducing power. Hence, these polysaccharides can be used as natural antioxidants in functional foods or medicine. Further experiments on the biological activities of these four polysaccharides are currently in progress.
Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.
Zuofa
Z
Zhang
Guoying
G
Lv
Weiqiang
W
He
Liangen
L
Shi
Huijuan
H
Pan
Leifa
L
Fan
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D055393:Flammulina / Q000737:chemistry* / Q000648:ultrastructure; D048690:Fruiting Bodies, Fungal / Q000737:chemistry* / Q000648:ultrastructure; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D006358:Hot Temperature; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors; D007502:Iron Chelating Agents / Q000737:chemistry / Q000302:isolation & purification*; D008855:Microscopy, Electron, Scanning; D008872:Microwaves; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010851:Picrates / Q000037:antagonists & inhibitors; D013010:Sonication; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water
D000975:Antioxidants; D001713:Biphenyl Compounds; D062610:Fungal Polysaccharides; D007502:Iron Chelating Agents; D010851:Picrates; D014867:Water; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl
Antioxidant activity; Extraction method; Flammulina velutipes; Polysaccharide