Surface chemistry architecture of silica nanoparticles determine the efficiency of in vivo fluorescence lymph node mapping.
Near-infrared (NIR) imaging of the lymphatic system offers a sensitive, versatile, and accurate lymph node mapping to locate the first, potentially metastatic, draining nodes in the operating room. Many luminescent nanoprobes have received great attention in this field, and the design of nontoxic and bright nanosystems is of crucial importance. Fluorescent NIR-emitting dye doped silica nanoparticles represent valuable platforms to fulfill these scopes, providing sufficient brightness, resistance to photobleaching, and hydrophilic nontoxic materials. Here, we synthesized these highly stable core-shell nanoparticles with a programmable surface charge positioning and determined the effect of these physicochemical properties on their in vivo behavior. In addition, we characterized their fluorescence kinetic profile in the right axillary lymph node (RALN) mapping. We found that nanoparticles with negative charges hidden by a PEG shell are more appropriate than those with external negative charges in the mapping of lymph nodes. We also demonstrated the efficient excretion of these nanostructures by the hepatobiliary route and their nontoxicity in mice up to 3 months postinjection. These results indicate the potential future development of these fluorescent nanosystems for LN mapping.
Centre de Recherche en Automatique de Nancy (CRAN), Université de Lorraine , UMR 7039, Campus Sciences, BP 70239, 54506 Vandoeuvre-lès-Nancy Cedex, France.
Marion
M
Helle
Enrico
E
Rampazzo
Morgane
M
Monchanin
Frédéric
F
Marchal
François
F
Guillemin
Sara
S
Bonacchi
Francesca
F
Salis
Luca
L
Prodi
Lina
L
Bezdetnaya
D000818:Animals; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D021701:Sentinel Lymph Node Biopsy / Q000379:methods*; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D014018:Tissue Distribution
D005456:Fluorescent Dyes; D012822:Silicon Dioxide