Optimization of nutrient component for diesel oil degradation by Acinetobacter beijerinckii ZRS.
A new bacterial strain that is capable of using diesel as the sole carbon source was isolated from the petroleum-contaminated soil of Xinjiang oil field, Northwest China. This bacterium was identified on the basis of its morphological and physiological characteristics and 16S rRNA gene sequence analysis, and it showed the greatest similarity with Acinetobacter beijerinckii 302-PWB-OH1 (99.86%). In order to enhance degradation efficiency, single-factor experiments and response surface methodology (RSM) were employed to optimize the nutrients used in artificial seawater. The results of this study revealed that 2.05 g L(-1) (NH4)2SO4, 1.46 g L(-1) Na2HPO4, and 14 mg L(-1) yeast extract in artificial seawater resulted in increasing the diesel degradation rate of the bacterial strain from 20.87% to 80.40% within 7 days. The actual experimental results were in agreement with the prediction.
College of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, China.
Lei
L
Huang
Jing
J
Xie
Bo-yi
BY
Lv
Xiao-feng
XF
Shi
Guo-qiang
GQ
Li
Feng-lai
FL
Liang
Jing-yan
JY
Lian
D000150:Acinetobacter / Q000502:physiology*; D001673:Biodegradation, Environmental; D002681:China; D052918:Environmental Restoration and Remediation / Q000379:methods*; D005742:Gasoline / Q000032:analysis*; D012987:Soil / Q000737:chemistry*; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*
D005742:Gasoline; D012987:Soil; D012989:Soil Pollutants
Acinetobacter beijerinckii; Bioremediation; Hydrocarbon degradation; Optimization; Response surface methodology