Effects of ophiopogonin D and spicatoside A derived from Liriope Tuber on secretion and production of mucin from airway epithelial cells.
In the present study, we investigated whether aqueous extract of Liriope Tuber, ophiopogonin D and spicatoside A derived from Liriope Tuber affect basal or phorbol ester (phorbol 12-myristate 13-acetate, PMA)-induced airway mucin production and secretion from airway epithelial cells. Confluent NCI-H292 cells were treated with each agent for 24 h (basal production) or pretreated with each agent for 30 min and then stimulated with PMA for 24 h (PMA-induced production and secretion), respectively. MUC5AC airway mucin production and secretion were measured by ELISA. The results were as follows: (1) aqueous extract of Liriope Tuber stimulated basal mucin production and did not inhibit but increased PMA-induced mucin production; (2) ophiopogonin D and spicatoside A stimulated basal mucin production and did not inhibit but increased PMA-induced mucin production; (3) two compounds increased PMA-induced mucin secretion. These results suggest that ophiopogonin D and spicatoside A can increase mucin production and secretion, by directly acting on airway epithelial cells and, at least in part, explain the traditional use of aqueous extract of Liriope Tuber as expectorants in diverse inflammatory pulmonary diseases.
Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon 303-131, Republic of Korea.
Su Hyun
SH
Park
Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon 303-131, Republic of Korea.
Hyun Jae
HJ
Lee
Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon 303-131, Republic of Korea.
Jiho
J
Ryu
Department of Food Science and Nutrition, Andong National University, Andong 760-749, Republic of Korea.
Kun Ho
KH
Son
Department of Food Science and Nutrition, Andong National University, Andong 760-749, Republic of Korea.
Sang Yong
SY
Kwon
Department of Pharmacy, College of Pharmacy, Seoul National University, Seoul 151-742, Republic of Korea.
Sang Kook
SK
Lee
Department of Pharmacy, College of Pharmacy, Seoul National University, Seoul 151-742, Republic of Korea.
Yeong Shik
YS
Kim
Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon 303-131, Republic of Korea.
Jang-Hee
JH
Hong
Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon 303-131, Republic of Korea.
Jeong Ho
JH
Seok
Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon 303-131, Republic of Korea. Electronic address: LCJ123@cnu.ac.kr.
Choong Jae
CJ
Lee
D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D031424:Liriope Plant / Q000737:chemistry*; D009077:Mucins / Q000096:biosynthesis / Q000378:metabolism*; D010703:Phorbol Esters / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000378:metabolism; D012503:Saponins / Q000378:metabolism* / Q000494:pharmacology*; D013150:Spirostans / Q000494:pharmacology*
C546003:5-(1-(glucopyranosyloxymethyl)ethenyl)-2-methyl-2-cyclohexen-1-one; D009077:Mucins; D010703:Phorbol Esters; D010936:Plant Extracts; D012503:Saponins; D013150:Spirostans; C046996:ophiopogonin D
Airway mucin; EGF; ELISA; Liriope tuber; Ophiopogonin D; PBS; PMA; RT-PCR; Spicatoside A; enzyme-linked immunosorbent assay; epidermal growth factor; phorbol 12-myristate 13-acetate; phosphate-buffered saline; reverse transcription-polymerase chain reaction