Chemical profiling of triacylglycerols and diacylglycerols in cow milk fat by ultra-performance convergence chromatography combined with a quadrupole time-of-flight mass spectrometry.
An ultra-performance convergence chromatography (UPC(2)) system coupled with quadrupole time-of-flight mass spectrometry (Q-TOF-MS) was successfully utilised to analyse triacylglycerols and diacylglycerols in cow milk fat. This novel approach obtained an improved resolution of triacylglycerols in comparison to previously reported chromatographic methods combined with MS detector in a shorter analytical time. A total of 49 triacylglycerols and 7 diacylglycerols were identified according to their secondary MS profiles and elementary composition. Furthermore, UPC(2) is an environmental friendly analytical method with a drastic reduction of organic solvent usage. The established UPC(2)-MS approach has potential application in lipidomics as an alternative method besides LC-MS and GC-MS.
Institute of Food and Nutraceutical Science, SJTU-Rich ResearchInstitute of Nutrition and Skin Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Qin
Q
Zhou
Boyan
B
Gao
Xi
X
Zhang
Yongwei
Y
Xu
Haiming
H
Shi
Liangli Lucy
LL
Yu
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004075:Diglycerides / Q000032:analysis*; D005223:Fats / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D014280:Triglycerides / Q000032:analysis*
D004075:Diglycerides; D005223:Fats; D014280:Triglycerides
Cow milk; Diacylglycerol; Quadrupole time-of-flight mass spectrometry; Triacylglycerol; Ultra-performance convergence chromatography