Simultaneous solid phase extraction and derivatization of aliphatic primary amines prior to separation and UV-absorbance detection.
To overcome the problem of poor sensitivity of capillary electrophoresis-UV absorbance for the detection of aliphatic amines, a solid phase extraction and derivatization scheme was developed. This work demonstrates successful coupling of amines to a chromophore immobilized on a solid phase and subsequent cleavage and analysis. Although the analysis of many types of amines is relevant for myriad applications, this paper focuses on the derivatization and separation of amines with environmental relevance. This work aims to provide the foundations for future developments of an integrated sample preparation microreactor capable of performing simultaneous derivatization, preconcentration, and sample cleanup for sensitive analysis of primary amines.
Department of Chemistry, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA.
Jessica L
JL
Felhofer
Karen
K
Scida
Mark
M
Penick
Peter A
PA
Willis
Carlos D
CD
Garcia
D000814:Aniline Compounds / Q000737:chemistry / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary; D008744:Methylamines / Q000737:chemistry / Q000302:isolation & purification*; D011437:Propylamines / Q000737:chemistry / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D013056:Spectrophotometry, Ultraviolet; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000302:isolation & purification*
D000814:Aniline Compounds; D008744:Methylamines; D011437:Propylamines; D014874:Water Pollutants, Chemical; C023650:aniline
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide; 2-(N-morpholino)ethanesulfonic acid hemisodium salt; 2-nitro-5-thiobenzoic acid; 3-mercaptopropyl-functionalized silica gel; 5,5′-dithiobis(2-nitrobenzoic acid); Amine; CE; Capillary electrophoresis; DTNB; Derivatization; EDAC; Extraction; MES; N-hydroxysuccinimide; NHS; NTB; SDS; Solid phase; TCEP; UV absorbance; capillary electrophoresis; sodium dodecyl sulfate; tris(2-carboxyethyl)phosphine; –SH particles