Hydrophobic variants of ganglio-tripod amphiphiles for membrane protein manipulation.
Membrane proteins operate in unique cellular environments. Once removed from their native context for the purification that is required for most types of structural or functional analyses, they are prone to denature if not properly stabilized by membrane mimetics. Detergent micelles have prominently been used to stabilize membrane proteins in aqueous environments as their amphipathic nature allows for shielding of the hydrophobic surfaces of these bio-macromolecules while supporting solubility and monodispersity in water. This study expands the utility of branched diglucoside-bearing tripod agents, designated ganglio-tripod amphiphiles, with introduction of key variations in their hydrophobic sections and shows how these latter elements can be fine-tuned to maximize membrane protein solubilization while preserving characteristics of these molecules that afford stabilization of rather fragile assemblies. Their efficacy rivals benchmark detergents heavily used today, such as n-dodecyl-β-d-maltoside.
Department of Bionano Engineering, Hanyang University, Ansan 426-791, Republic of Korea. Electronic address: pchae@hanyang.ac.kr.
Pil Seok
PS
Chae
Kyung Ho
KH
Cho
Marc J
MJ
Wander
Hyoung Eun
HE
Bae
Samuel H
SH
Gellman
Philip D
PD
Laible
D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002458:Cell Fractionation; D057446:Hydrodynamics; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification*; D008823:Micelles; D016354:Rhodobacter capsulatus / Q000737:chemistry* / Q000187:drug effects; D012995:Solubility; D013501:Surface-Active Agents / Q000737:chemistry* / Q000494:pharmacology; D014867:Water / Q000737:chemistry
D001426:Bacterial Proteins; D008565:Membrane Proteins; D008823:Micelles; D013501:Surface-Active Agents; D014867:Water
Amphiphile; Membrane mimetic; Membrane protein; Molecular design; Protein solubilization