Reflecting on confocal microscopy: a personal perspective.
The first practical laser scanning confocal microscopes were introduced to the biomedical community over 30 years ago. Their subsequent development continues to influence the introduction of new methods and applications of optical sectioning microscopy.
Laboratory for Optical and Computational Instrumentation and Laboratory of Molecular Biology, University of Wisconsin, Madison, WI, USA.
John
J
White
D005453:Fluorescence; D006801:Humans; D021621:Imaging, Three-Dimensional; D018613:Microscopy, Confocal / Q000379:methods*