[Increased level of lipid peroxidation products and disturbances in oxidation-reduction balance in erythrocytes from patients suffering from systemic sclerosis, who are chronically treated with vitamin E].
Systemic sclerosis is a chronic connective tissue disease of unknown pathogenesis. In view of the reports of essential role of oxidative stress in development of disease, trials with supportive care with vitamin E are undertaken. The aim of the study was to estimate parameters of oxidation-reduction balance in erythrocytes from scleroderma patients, who were chronically treated with vitamin E compared with healthy controls.


METHODS
In the study there were included 14 women with systemic sclerosis (limited form - ISSc - n = 10, diffuse form - dSsc - n = 4, age 53.8 lat +/- 11.5), who were treated with vitamin E in dose 400 mg/day not shorter than in 6 months period and 23 healthy women (age 52.7 +/- 11.2) as a control group. The following measurements were done: hs CRP (immunoturbidimetic method), glutathione peroxidase activity (Gpx--method of Rice-Evans, 1991), superoxide dismutase activity (SOD--method of Misra, 1972), catalase activity (CAT--method of Aebi H, 1984), free thiol group concentration (SH--method of ElIman, 1959), level of lipid peroxidation products (TBARs--method of Stocks and Dormandy, 1971), total antioxidant capacity (TAC) depended of slow (TAC "slow") and fast (TAC "fast") antioxidants.


RESULTS
. In both forms of systemic sclerosis significantly higher TBARs in comparison of healthy controls (5.81 +/- 1.57 vs 4.28 +/- 0.89 nM TBARS/gHb; p < 0.01) was observed. Patients with limited systemic sclerosis have significantly higher activity of Gpx (59.9 +/- 26.11 vs 32.19 +/- 11.67 U/mg Hb; p < 0.01), and no differences in activity of CAT and SOD. In patients with diffuse systemic sclerosis significantly lower activity of CAT (173.06 +/- 60.3 vs 284.47 +/- 43.33 U/mg Hb; p < 0.01) and SOD (2334.95 +/- 193.97 vs 3231.47 +/- 840.21 U/mg Hb; p < 0.05) was observed. There are no differences in TAC and SH between investigated groups.


CONCLUSIONS
In scleroderma patients despite chronical treatment with vitamin E, oxidation-reduction balance disturbances are observed in the form of increased level of lipid peroxidation products. Besides, a lower activity of catalase and superoxide dysmutase in patients who suffer from diffuse form of systemic sclerosis is noted. Patients with limited systemic sclerosis have higher glutathione peroxidase activity.
Uniwersytet Medyczny w Łodzi, Klinika Chorób Wewnetrznych i Farmakologii Klinicznej. kasia.dworniak@gmail.com
Katarzyna
K
Dworniak
Piotr
P
Duchnowicz
Maria
M
Koter-Michalak
Bozena
B
Dziankowska-Bartkowiak
Zofia
Z
Gerlicz
Elzbieta
E
Waszczykowska
Marlena
M
Broncel
D002374:Catalase / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D012595:Scleroderma, Systemic / Q000097:blood* / Q000188:drug therapy*; D013482:Superoxide Dismutase / Q000378:metabolism; D014810:Vitamin E / Q000627:therapeutic use*
D014810:Vitamin E; D002374:Catalase; D013482:Superoxide Dismutase