Generation of site-appropriate tissue by a living cellular sheet in the treatment of mucogingival defects.
BACKGROUND
Generation of site-appropriate tissue in the oral cavity includes the restoration of the correct anatomic type, amount, and distribution of the tissue. This study is a post hoc analysis of data collected during previously published results from two randomized clinical trials of a living cellular sheet (LCS; allogenic cultured keratinocytes and fibroblasts in bovine collagen) versus a free gingival graft (FGG), evaluating their ability to augment keratinized tissue or gingiva.


METHODS
Post hoc histologic and clinical (photographic) comparisons of the outcomes of treatment were performed on histologic and photographic data gathered in the two randomized clinical trials.


RESULTS
Histologic findings showed that LCS-treated sites resembled gingiva rather than alveolar mucosa. Photographic analysis indicated that LCS treatment resulted in more site-appropriate tissue than FGG in terms of tissue color, with adjacent untreated tissue, absence of scar formation or keloid-like appearance, and mucogingival junction alignment.


CONCLUSIONS
Treatment of mucogingival defects with LCS resulted in the generation of tissue that is more site appropriate than tissue transplanted from the palate.
Private practice, Houston, TX.
E Todd
ET
Scheyer
Marc L
ML
Nevins
Rodrigo
R
Neiva
David L
DL
Cochran
William V
WV
Giannobile
Sook-Bin
SB
Woo
William N
WN
King
John K
JK
Spitznagel
Damien
D
Bates
Michael K
MK
McGuire
D064591:Allografts / Q000473:pathology / Q000637:transplantation*; D000818:Animals; D064592:Autografts / Q000637:transplantation; D001706:Biopsy / Q000379:methods; D002417:Cattle; D002921:Cicatrix / Q000517:prevention & control; D003094:Collagen; D003116:Color; D004847:Epithelial Cells / Q000473:pathology; D004955:Esthetics, Dental; D005347:Fibroblasts / Q000473:pathology / Q000637:transplantation*; D005500:Follow-Up Studies; D005881:Gingiva / Q000473:pathology / Q000637:transplantation*; D005882:Gingival Diseases / Q000473:pathology / Q000601:surgery*; D006801:Humans; D007627:Keloid / Q000517:prevention & control; D015603:Keratinocytes / Q000473:pathology / Q000637:transplantation*; D007633:Keratins; D009061:Mouth Mucosa / Q000473:pathology; D010781:Photography / Q000379:methods; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D016896:Treatment Outcome
D007633:Keratins; D003094:Collagen