Genetic marker suitable for identification and genotyping of Plasmodium ovale curtisi and Plasmodium ovale wallikeri.
We present a seminested PCR method that specifically discriminates between Plasmodium ovale curtisi and P. ovale wallikeri with high sensitivity. The test is based on species-specific amplification of a size-polymorphic fragment of the tryptophan-rich antigen gene, potra, which also permits discrimination of intraspecific sequence variants at this locus.
Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Naowarat
N
Tanomsing
Mallika
M
Imwong
Colin J
CJ
Sutherland
Christiane
C
Dolecek
Tran Tinh
TT
Hien
Francois
F
Nosten
Nicholas P J
NP
Day
Nicholas J
NJ
White
Georges
G
Snounou
D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics*; D017125:Genes, Protozoan; D005819:Genetic Markers; D008969:Molecular Sequence Data; D010274:Parasitology / Q000379:methods*; D041122:Plasmodium ovale / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
D016054:DNA, Protozoan; D005819:Genetic Markers