A new, rapid and sensitive bioluminescence assay for drug screening on Leishmania.
We validated a new method, based on luciferine/luciferase bioluminescence, for drug screening on promastigotes of different Leishmania species. Results obtained with this new, rapid, reproducible, and reliable method are in good accordance with results obtained by the conventional MTT assay. This bioluminescence assay has a lower detection limit.
Infections Parasitaires, Transmission, Physiopathologie et Thérapeutique, UMR MD3, Faculté de Pharmacie, Aix-Marseille Université, 27 Boulevard Jean Moulin, CS 30064, 13385 Marseille Cedex 05, France. Electronic address: lupaloque@hotmail.com.
Lucie
L
Paloque
Nicolas
N
Vidal
Magali
M
Casanova
Aurélien
A
Dumètre
Pierre
P
Verhaeghe
Daniel
D
Parzy
Nadine
N
Azas
D000981:Antiprotozoal Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D049454:Firefly Luciferin / Q000378:metabolism; D007891:Leishmania / Q000187:drug effects*; D049409:Luciferases, Firefly / Q000032:analysis; D008163:Luminescent Measurements / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000981:Antiprotozoal Agents; D049454:Firefly Luciferin; D049409:Luciferases, Firefly
Accuracy; Assay validation; Leishmania; Linearity; Luminescence; Screening