Physiological concentrations of leptin do not affect human neutrophils.
Leptin is an adipokine that is thought to be important in many inflammatory diseases, and is known to influence the function of several leukocyte types. However, no clear consensus is present regarding the responsiveness of neutrophils for this adipokine. In this study a 2D DIGE proteomics approach was used as an unbiased approach to identify leptin-induced effects on neutrophils. Additionally chemotaxis and survival experiments were performed to reproduce results from literature showing putative effects of leptin on these neutrophil responses. Leptin did not induce any significant changes in the proteome provided leptin was added at physiologically relevant concentrations (250 ng). Our leptin batches were biologically active as they induced proliferation in LeptinR expressing Ba/F3 cells. At high concentrations (25000 ng) leptin induced a change in neutrophil proteome. Seventeen differently regulated spots were identified of which twelve could be characterized by mass spectrometry. Two of these identified proteins, SerpinB1 and p40 phox, were chosen for further analysis but leptin-induced expression analyzed by western blot were highly variable. Additionally leptin also induced neutrophil survival at these high concentrations. No leptin-induced chemotaxis of human neutrophils was detected at any concentration. In conclusion, physiological concentrations of leptin do not affect neutrophils. High leptin concentrations induced survival and changes in the neutrophils proteome, but this was most likely mediated by an indirect effect. However, it cannot be ruled out that the effects were mediated by a yet not-identified leptin receptor on human neutrophils.
Department of Respiratory Medicine, University Medical Centre Utrecht, Utrecht, The Netherlands.
Vera M
VM
Kamp
Jeroen D
JD
Langereis
Corneli W
CW
van Aalst
Jan A
JA
van der Linden
Laurien H
LH
Ulfman
Leo
L
Koenderman
D015153:Blotting, Western; D039481:CD11b Antigen / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D005434:Flow Cytometry; D006801:Humans; D020738:Leptin / Q000494:pharmacology*; D013058:Mass Spectrometry; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D015843:Serpins / Q000378:metabolism
D039481:CD11b Antigen; D020738:Leptin; D015843:Serpins; C075177:SERPINB1 protein, human