A galactomannan polysaccharide from Punica granatum imparts in vitro and in vivo anticancer activity.
Galactomannan polysaccharide (PSP001) was isolated from the fruit rind of Punica granatum and was previously reported to have excellent antioxidant and immunomodulatory properties. The cytotoxicity of PSP001 was evaluated in the human cancer cell lines A375, HCT116, and HepG2 as well as the murine cancer cell lines DLA and EAC over a wide range of concentrations. PSP001 exhibited significant cytotoxicity against cancer cells through the induction of apoptosis with no in vivo toxicity up to a concentration of 2000 mg/kg body weight when assessed in BALB/c mice. The antitumor efficacy of PSP001 was tested in DLA and EAC murine ascites and EAC solid tumor mouse models. PSP001 alone and in combination with doxorubicin produced a significant reduction in the tumor burden and increased life span in both models compared to the controls. The results suggest that PSP001 has the potential to be developed as an anticancer agent either alone or as an adjuvant to chemotherapy.
Division of Cancer Research, Regional Cancer Centre, Thiruvananthapuram 695 011, Kerala, India. Electronic address: manumjoseph2000@yahoo.com.
Manu M
MM
Joseph
S R
SR
Aravind
Suraj K
SK
George
Sheeja
S
Varghese
T T
TT
Sreelekha
D000473:Alkanes; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology*; D004357:Drug Synergism; D005260:Female; D005638:Fruit / Q000737:chemistry*; D005690:Galactose / Q000031:analogs & derivatives; D006801:Humans; D029561:Lythraceae / Q000737:chemistry*; D008351:Mannans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000432:Methanol; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000737:chemistry*; D012997:Solvents; D016019:Survival Analysis; D047368:Tumor Burden / Q000187:drug effects
D000473:Alkanes; D000972:Antineoplastic Agents, Phytogenic; D008351:Mannans; D010936:Plant Extracts; D012997:Solvents; C012990:galactomannan; D004317:Doxorubicin; C004544:naphtha; D005690:Galactose; D000432:Methanol
Cancer; Dalton's lymphoma ascites; Ehrlich's ascites carcinoma; Polysaccharide; Punica granatum