Intra-arterial infusion of thrombin: animal experiments.
OBJECTIVE
Thrombin inhibits cadherin on vascular endothelial cells, rapidly and reversibly increasing endothelial permeability. The purpose of this study was to evaluate the feasibility of trans-arterial infusion with thrombin.


METHODS
Ten rabbits with right thigh tumor were randomly divided into two groups: A thrombin group and a control group. In the thrombin group, a suspension of thrombin (300 IU), cisplatin (3 mg), lipiodol (0.3 ml) and iopamidol (0.3 ml) was infused into the right femoral artery. In the control group, a suspension of cisplatin, lipiodol and iopamidol was infused. Platinum concentrations in plasma were measured five and ten minutes after administration. Platinum concentrations were also measured in tumor specimens excised 30 minutes after infusion.


RESULTS
At both five and ten minutes after infusion, platinum concentrations in plasma were significantly lower for the thrombin group than for the control group. Platinum concentration in tumor tissue was significantly higher for the thrombin group than for the control group.


CONCLUSIONS
The present results suggest that transarterial infusion with thrombin may offer a number of pharmacological advantages.
Kansai Medical University, Department of Radiology , Osaka , Japan.
Atsushi
A
Komemushi
Shuji
S
Kariya
Miyuki
M
Nakatani
Rie
R
Yoshida
Yumiko
Y
Kono
Satoshi
S
Suzuki
Shohei
S
Kanno
Koshi
K
Ikeda
Keita
K
Utsunomiya
Yoko
Y
Harima
Kazuhisa
K
Oyamada
Tsuyoshi
T
Kamiyoshi
Tsuyoshi
T
Miyake
Ryo
R
Sato
Michito
M
Inoue
Noboru
N
Tanigawa
D032761:Animal Experimentation; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D002945:Cisplatin / Q000008:administration & dosage / Q000493:pharmacokinetics; D003287:Contrast Media / Q000008:administration & dosage; D016503:Drug Delivery Systems; D004998:Ethiodized Oil / Q000008:administration & dosage / Q000493:pharmacokinetics; D005240:Feasibility Studies; D005263:Femoral Artery; D007261:Infusions, Intra-Arterial; D007479:Iopamidol / Q000008:administration & dosage / Q000493:pharmacokinetics; D008114:Liver Neoplasms, Experimental / Q000473:pathology; D008297:Male; D010984:Platinum / Q000493:pharmacokinetics*; D011817:Rabbits; D013917:Thrombin / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors
D000970:Antineoplastic Agents; D003287:Contrast Media; D010984:Platinum; D004998:Ethiodized Oil; D013917:Thrombin; D007479:Iopamidol; D002945:Cisplatin