Highly selective fluorescent sensing of proteins based on a fluorescent molecularly imprinted nanosensor.
A fluorescent molecularly imprinted nanosensor was obtained by grafting imprinted polymer onto the surface of multi-wall carbon nanotubes and post-imprinting treatment with fluorescein isothiocyanate (FITC). The fluorescence of lysozyme-imprinted polymer (Lys-MIP) was quenched more strongly by Lys than that of nonimprinted polymer (NIP), which indicated that the Lys-MIP could recognize Lys. The resulted imprinted material has the ability to selectively sense a target protein, and an imprinting factor of 3.34 was achieved. The Lys-MIP also showed selective detection for Lys among other proteins such as cytochrome C (Cyt C), hemoglobin (HB) and bovine serum albumin (BSA) due to the imprinted sites in the Lys-MIP. This approach combines the high selectivity of surface molecular imprinting technology and fluorescence, and converts binding events into detectable signals by monitoring fluorescence spectra. Therefore, it will have further applications for Lys sensing.
Department of Science, Tianjin University of Science and Technology, Tianjin 300457, China. S.wang@tust.edu.cn.
Qiliang
Q
Deng
Jianhua
J
Wu
Xiaorui
X
Zhai
Guozhen
G
Fang
Shuo
S
Wang
D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D054802:Molecular Imprinting / Q000295:instrumentation*; D009113:Muramidase / Q000032:analysis* / Q000737:chemistry; D036103:Nanotechnology / Q000295:instrumentation*; D040081:Protein Array Analysis / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
D009113:Muramidase