Mitochondria-targeted reaction-based two-photon fluorescent probe for imaging of superoxide anion in live cells and in vivo.
A newly synthesized reaction-based two-photon (TP) fluorescence imaging probe, 9-butyltriphenylphosphoniumacylamino-2,7-dibenzothiazolineflurene (MF-DBZH), composed of a superoxide anion (O2(•-)) responsive group and a mitochondria-targeted site, has been shown to have high selectivity toward mitochondrial O2(•-) fluxes. The fluorescence intensity of MF-DBZH responds proportionally to changes in O2(•-) concentrations. Moreover, MF-DBZH was proved to be insensitive toward pH changes and has high photostability. Favorable features of this probe also include convenient cell loading, easy staining of both cells and small animals, and excellent biocompibility. Most importantly, MF-DBZH gives reliable TP fluorescent signal to changes of O2(•-) levels in vivo.
College of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Ministry of Education, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University , Jinan, Shandong 250014, People's Republic of China.
Ping
P
Li
Wen
W
Zhang
Kexiang
K
Li
Xiao
X
Liu
Haibin
H
Xiao
Wei
W
Zhang
Bo
B
Tang
D000818:Animals; D052160:Benzothiazoles / Q000737:chemistry*; D016466:CHO Cells; D002470:Cell Survival; D006224:Cricetinae; D003412:Cricetulus; D005449:Fluorenes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D008928:Mitochondria / Q000378:metabolism*; D017785:Photons; D013481:Superoxides / Q000737:chemistry* / Q000378:metabolism*; D013844:Thiazoles / Q000737:chemistry*
C000590682:9-butyltriphenylphosphoniumacylamino-2,7-dibenzothiazolineflurene; D052160:Benzothiazoles; D005449:Fluorenes; D005456:Fluorescent Dyes; D013844:Thiazoles; D013481:Superoxides