Improved quality control method for prescriptions of Polygonum capitatum through simultaneous determination of nine major constituents by HPLC coupled with triple quadruple mass spectrometry.
As a traditional Miao-nationality medicinal plant, Polygonum capitatum has been used in clinical practice for several thousand years. Its prescriptions, including three dosage forms: granules, capsule and tablet are known by the brand name Relinqing® and have played an indispensable role in the treatment of urinary system infection, pyelonephritis and kidney stones. However, no study about the comprehensive quality evaluation of Relinqing® has been reported. In the present paper, a method for the simultaneous determination of nine major compounds in three dosage forms of Relinqing® using HPLC coupled with triple quadrupole mass spectrometry (HPLC-QQQ MS) was established to comprehensively evaluate their quality. The nine compounds, including four phenolic acids, four flavonoids and a lignin, were analyzed with acceptable linear regression relationship (r², 0.9923-0.9992), precision (RSD, 1.25%-2.78%), repeatability (RSD, 2.05%%-3.47%), stability (RSD, 1.84%-3.72%) and recovery (93.60%-108.54%, RSD ≤ 3.67%). The present study fills the gap in the multivariate quality control of Relinqing® and provides a valuable reference for quality standards and dosage reforming of this traditional Chinese medicine.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. liuan@icmm.ac.cn.
Kai-Xia
KX
Zhang
Yue-Sheng
YS
Wang
Wen-Guang
WG
Jing
Jun
J
Zhang
An
A
Liu
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D006801:Humans; D008516:Medicine, Chinese Traditional; D028502:Polygonum / Q000737:chemistry*; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000592:standards
D004365:Drugs, Chinese Herbal