Guava pomace: a new source of anti-inflammatory and analgesic bioactives.
BACKGROUND
Guava pomace is an example of the processing waste generated after the manufacturing process from the juice industry that could be a source of bioactives. Thus, the present investigation was carried out in order to evaluate the anti-inflammatory and antinociceptive potential and determinate the main phenolic compounds of a guava pomace extract (GPE).


METHODS
The anti-inflammatory activity was evaluated by carrageenan, dextran, serotonin, histamine-induced paw edema and neutrophils migration in the peritoneal cavity models. Acetic acid-induced abdominal writhing and formalin test were performed to investigate the antinociceptive effects. In addition, the content of total phenolic and of individual phenolic compounds was determined by GC/MS.


RESULTS
GPE showed anti-inflammatory activity by carrageenan, dextran, serotonin, histamine-induced paw edema and neutrophils migration in the peritoneal cavity models (p < 0.05). GPE also demonstrated antinociceptive activity by acetic acid-induced abdominal writhing and formalin test (p < 0.05). The total phenolic value was 3.40 ± 0.09 mg GAE/g and epicatechin, quercetin, myricetin, isovanilic and gallic acids were identified by GC/MS analysis.


CONCLUSIONS
The presence of bioactive phenolic compounds as well as important effects demonstrated in animal models suggest that guava pomace could be an interesting source of anti-inflammatory and analgesic substances.
Department of Physiological Sciences, Piracicaba Dental School, University of Campinas - UNICAMP, P,O, Box 52, 13414-018 Piracicaba, SP, Brazil. dennycarina@hotmail.com.
Carina
C
Denny
Priscilla S
PS
Melo
Marcelo
M
Franchin
Adna P
AP
Massarioli
Keityane B
KB
Bergamaschi
Severino M
SM
de Alencar
Pedro L
PL
Rosalen
D000700:Analgesics / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects; D005557:Formaldehyde; D007220:Industrial Waste; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils; D010147:Pain Measurement; D010529:Peritoneal Cavity / Q000166:cytology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D031647:Psidium / Q000737:chemistry*
D000700:Analgesics; D000893:Anti-Inflammatory Agents; D007220:Industrial Waste; D010936:Plant Extracts; D005557:Formaldehyde