VEGF promotes cardiac stem cells differentiation into vascular endothelial cells via the PI3K/Akt signaling pathway.
Recent research suggested that cardiac stem cells (CSCs) may have the clinical application for cardiac repair. However, their characteristics and the regulatory mechanisms of their growth and differentiation have not been fully investigated. Vascular endothelial growth factor (VEGF, VEGF-A) is a major regulator of physiological and pathological angiogenesis. But the homing role of VEGF for CSCs is unclear. In this report, CSCs were isolated, purified, and expanded in vitro from rat heart. VEGF, SU5416 (VEGF receptor blocker), and Wortmannin (PI3K/Akt signaling pathway inhibitor) were used for differentiation into vascular endothelial cells (VECs). Real-time qPCR was selected to confirm the role of PI3K/Akt signaling pathway in VECs differentiation from rat CSCs. The result of real-time qPCR demonstrated that PI3K/Akt signaling pathway plays an important role in rat CSCs differentiated into VECs. So, our research provides a theoretical basis and experimental evidence for therapeutic application of rat CSCs to treat cardiac repair.
Department of Internal Medicine, Hebei Medical University , Shijiazhuang , P. R. China.
Nuan
N
Xiao
Xiao-Yong
XY
Qi
Lu-Ning
LN
Tang
Li-Li
LL
Tan
Ya-Qing
YQ
Chen
Hong-Mei
HM
Zhao
D053687:Adult Stem Cells / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000502:physiology*; D007211:Indoles / Q000494:pharmacology; D032386:Myoblasts, Cardiac / Q000166:cytology*; D009206:Myocardium / Q000166:cytology*; D051058:Oncogene Protein v-akt / Q000378:metabolism; D010641:Phenotype; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D011758:Pyrroles / Q000494:pharmacology; D051381:Rats; D040262:Receptors, Vascular Endothelial Growth Factor / Q000037:antagonists & inhibitors; D015398:Signal Transduction; D033581:Stem Cell Transplantation; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D014945:Wound Healing
D007211:Indoles; D011758:Pyrroles; D042461:Vascular Endothelial Growth Factor A; C116890:Semaxinib; D019869:Phosphatidylinositol 3-Kinases; D040262:Receptors, Vascular Endothelial Growth Factor; D051058:Oncogene Protein v-akt
PI3K/Akt signaling pathway; cardiac stem cells; rat; vascular endothelial cells