Reactivation of plasma butyrylcholinesterase by pralidoxime chloride in patients poisoned by WHO class II toxicity organophosphorus insecticides.
Some clinicians assess the efficacy of pralidoxime in organophosphorus (OP) poisoned patients by measuring reactivation of butyrylcholinesterase (BuChE). However, the degree of BuChE inhibition varies by OP insecticide, and it is unclear how well oximes reactivate BuChE in vivo. We aimed to assess the usefulness of BuChE activity to monitor pralidoxime treatment by studying its reactivation after pralidoxime administration to patients with laboratory-proven World Health Organization (WHO) class II OP insecticide poisoning. Patient data were derived from 2 studies, a cohort study (using a bolus treatment of 1g pralidoxime chloride) and a randomized controlled trial (RCT) (comparing 2g pralidoxime over 20 min, followed by an infusion of 0.5 g/h, with placebo). Two grams of pralidoxime variably reactivated BuChE in patients poisoned by 2 diethyl OP insecticides, chlorpyrifos and quinalphos; however, unlike acetylcholinesterase reactivation, this reactivation was not sustained. It did not reactivate BuChE inhibited by the dimethyl OPs dimethoate or fenthion. The 1-g dose produced no reactivation. Pralidoxime produced variable reactivation of BuChE in WHO class II OP-poisoned patients according to the pralidoxime dose administered, OP ingested, and individual patient. The use of BuChE assays for monitoring the effect of pralidoxime treatment is unlikely to be clinically useful.
* Department of Pharmacology, Toxicology, and Therapeutics, University/BHF Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, EH16 4TJ, UK;
Lisa A
LA
Konickx
Franz
F
Worek
Shaluka
S
Jayamanne
Horst
H
Thiermann
Nicholas A
NA
Buckley
Michael
M
Eddleston
D000931:Antidotes / Q000627:therapeutic use*; D002091:Butyrylcholinesterase / Q000097:blood*; D015331:Cohort Studies; D004793:Enzyme Reactivators / Q000627:therapeutic use*; D006801:Humans; D007306:Insecticides / Q000506:poisoning*; D062025:Organophosphate Poisoning / Q000188:drug therapy*; D011220:Pralidoxime Compounds / Q000627:therapeutic use*
D000931:Antidotes; D004793:Enzyme Reactivators; D007306:Insecticides; D011220:Pralidoxime Compounds; D002091:Butyrylcholinesterase; C028797:pralidoxime
butyrylcholinest erase; human poisoning.; marker; organophosphorus insecticides; pralidoxime