Effects of organic loading rates on reactor performance and microbial community changes during thermophilic aerobic digestion process of high-strength food wastewater.
To evaluate the applicability of single-stage thermophilic aerobic digestion (TAD) process treating high-strength food wastewater (FWW), TAD process was operated at four organic loading rates (OLRs) from 9.2 to 37.2 kg COD/m(3)d. The effects of OLRs on microbial community changes were also examined. The highest volumetric removal rate (13.3 kg COD/m(3)d) and the highest thermo-stable protease activity (0.95 unit/mL) were detected at OLR=18.6 kg COD/m(3)d. Denaturing gradient gel electrophoresis (DGGE) profiles and quantitative PCR (qPCR) results showed significant microbial community shifts in response to changes in OLR. In particular, DGGE and phylogenetic analysis demonstrate that the presence of Bacillus sp. (phylum of Firmicutes) was strongly correlated with efficient removal of organic particulates from high-strength food wastewater.
School of Environmental Science and Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Pohang 790-784, South Korea.
Hyun Min
HM
Jang
Jae Won
JW
Lee
Jeong Hyub
JH
Ha
Jong Moon
JM
Park
D000332:Aerobiosis / Q000187:drug effects; D000468:Alkalies / Q000737:chemistry; D001419:Bacteria / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*; D001483:Base Sequence; D001673:Biodegradation, Environmental / Q000187:drug effects; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D005502:Food; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008055:Lipids / Q000032:analysis; D009584:Nitrogen / Q000032:analysis; D009930:Organic Chemicals / Q000494:pharmacology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012995:Solubility / Q000187:drug effects; D013696:Temperature; D014835:Volatilization / Q000187:drug effects; D062065:Wastewater / Q000382:microbiology*; D018508:Water Purification / Q000379:methods*
D000468:Alkalies; D004269:DNA, Bacterial; D008055:Lipids; D009584:Nitrogen; D009930:Organic Chemicals; D012336:RNA, Ribosomal, 16S; D062065:Wastewater
Food wastewater; Microbial community; Organic loading rate (OLR); Protease activity; Thermophilic aerobic digestion (TAD)