Molecularly imprinted micro solid-phase extraction technique coupled with complementary molecularly imprinted polymer-sensor for ultra trace analysis of epinephrine in real samples.
A simple hyphenation approach was adopted to obtain a new molecularly imprinted micro solid-phase extraction fiber (as a selective extraction tool) and complementary molecularly imprinted polymer coated pencil graphite electrode (as a detection tool) for the selective and sensitive analysis of epinephrine, which is a disease biomarker prevalent at ultra trace level in biological fluids. In both extraction and detection processes, the functionalized multiwalled carbon nanotubes (CNT-mers) were preferred to multiwalled carbon nanotubes (unmodified) in order to obtain a stable homogeneously dispersed imprinted polymer matrix of better electroconductivity and adsorptive characteristics. The hyphenation of both tools helped dual pre-concentration of epinephrine so as to achieve the stringent limit [limit of detection: 0.002 ng mL(-1), S/N=3] of clinical detection, without any problems of non-specific contributions and cross-reactivity.
Analytical Division, Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: prof.bbpd@yahoo.com.
Bhim Bali
BB
Prasad
Analytical Division, Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
Amrita
A
Srivastava
Analytical Division, Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
Amrita
A
Prasad
Analytical Division, Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: mahavirtiwari@gmail.com.
Mahavir Prasad
MP
Tiwari
D004837:Epinephrine / Q000097:blood* / Q000134:cerebrospinal fluid*; D006801:Humans; D054802:Molecular Imprinting; D037742:Nanotubes, Carbon / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*
D037742:Nanotubes, Carbon; D011108:Polymers; D004837:Epinephrine
CNT-mer; Differential pulse anodic stripping voltammetry; Epinephrine; Molecularly imprinted micro solid-phase extraction; Real sample analysis