Quantitative determination of plant phenolics in Urtica dioica extracts by high-performance liquid chromatography coupled with tandem mass spectrometric detection.
A method for quantification of 45 plant phenolics (including benzoic acids, cinnamic acids, flavonoid aglycones, C- and O-glycosides, coumarins, and lignans) in plant extracts was developed, based on reversed phase HPLC separation of extract components, followed by tandem mass spectrometric detection. The phenolic profile of 80% MeOH extracts of the stinging nettle (Urtica dioica L.) herb, root, stem, leaf and inflorescence was obtained by using this method. Twenty-one of the investigated compounds were present at levels above the reliable quantification limit, with 5-O-caffeoylquinic acid, rutin and isoquercitrin as the most abundant. The inflorescence extracts were by far the richest in phenolics, with the investigated compounds amounting 2.5-5.1% by weight. As opposed to this, the root extracts were poor in phenolics, with only several acids and derivatives being present in significant amounts. The results obtained by the developed method represent the most detailed U. dioica chemical profile so far.
Department of Chemistry, Biochemistry and Environmental Protection, University of Novi Sad Faculty of Sciences, Trg Dositeja Obradovića 3, 21000 Novi Sad, Serbia. Electronic address: dejan.orcic@dh.uns.ac.rs.
Dejan
D
Orčić
Marina
M
Francišković
Kristina
K
Bekvalac
Emilija
E
Svirčev
Ivana
I
Beara
Marija
M
Lesjak
Neda
N
Mimica-Dukić
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010636:Phenols / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D026982:Urtica dioica / Q000737:chemistry*
D010636:Phenols; D010936:Plant Extracts
LC–MS/MS; Method validation; Plant phenolics; Stinging nettle; Urtica dioica