Online capillary electrophoresis for monitoring carboxylic acid production by yeast during bioreactor cultivations.
Bioprocess monitoring can improve the understanding and control of biotechnological processes. When analyses are carried out as automated online measurements, manual steps of the analysis procedures are avoided, thus decreasing both the time required for analyses and systematic errors. In this study, an online capillary electrophoresis (CE) system with flow-through sample vial made in-house and action control programming was assembled to monitor carboxylic acid production by Kluyveromyces lactis and Saccharomyces cerevisiae during two different bioreactor cultivations. The relative standard deviations were less than 0.6% for intraday migration times and the total analysis time was less than 20 min. The system operated continuously and automatically up to 6 days and produced data concerning carboxylic acid production during the cultivations. The successful test runs demonstrated that this system has potential for the monitoring of biotechnological processes.
VTT Technical Research Centre of Finland , P.O. Box 1000, FIN-02044 VTT Espoo, Finland.
Heidi
H
Turkia
Sami
S
Holmström
Toni
T
Paasikallio
Heli
H
Sirén
Merja
M
Penttilä
Juha-Pekka
JP
Pitkänen
D019149:Bioreactors / Q000382:microbiology*; D002264:Carboxylic Acids / Q000378:metabolism*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007716:Kluyveromyces / Q000254:growth & development / Q000378:metabolism*; D009862:Online Systems; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*
D002264:Carboxylic Acids