HLA-G regulates the invasive properties of JEG-3 choriocarcinoma cells by controlling STAT3 activation.
The expression of human leucocyte antigen-G (HLA-G) in trophoblasts plays a crucial role in successful embryonic implantation, and reduced HLA-G expression might contribute to adverse obstetric outcomes. In this study, we silenced HLA-G expression using RNA interference in JEG-3 cells, resulting in a notably attenuated invasion capacity of the cells in a Transwell assay; however, no alterations in cell proliferation or apoptosis were observed. The down-regulation of HLA-G dampened the activation of signal transducer and activator of transcription 3 (STAT3), whereas the up-regulation of HLA-G promoted STAT3 activation and invasion in JEG-3 cells treated with human galectin-1. Most importantly, interleukin-6 (IL-6), but not galectin-1, was shown to rescue invasion deficiency in a dose-dependent manner. Thus, we demonstrate that HLA-G is able to regulate JEG-3 cell invasion by influencing STAT3 activation, which may underlie the implantation defects accompanying HLA-G hypo-expression in pre-eclampsia.
Obstetrics and Gynecology Hospital, Fudan University, Shanghai, PR China; Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, PR China.
X
X
Liu
W
W
Gu
X
X
Li
D045744:Cell Line, Tumor; D002465:Cell Movement; D002822:Choriocarcinoma / Q000378:metabolism* / Q000473:pathology; D015536:Down-Regulation; D010064:Embryo Implantation; D005260:Female; D037483:Galectin 1 / Q000235:genetics / Q000378:metabolism; D059951:HLA-G Antigens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering; D011994:Recombinant Proteins / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000819:agonists / Q000378:metabolism*; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation; D014594:Uterine Neoplasms / Q000378:metabolism* / Q000473:pathology
D037483:Galectin 1; D059951:HLA-G Antigens; C508600:IL6 protein, human; D015850:Interleukin-6; C445499:LGALS1 protein, human; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human
HLA-G; Human galectin-1; IL-6; JEG-3; STAT3; Trophoblast invasion