Protective effect of fermented soybean dried extracts against TPA-induced oxidative stress in hairless mice skin.
This study evaluated the chemical properties (polyphenol and genistein contents) of soybean extracts obtained by biotransformation and dried by spray dryer at different conditions and their in vivo ability to inhibit 12-O-tetradecanoylphorbol-13-acetate- (TPA-) induced biochemical alterations in the skin of hairless mice. By comparing the obtained data with that of the well-known active soybean extract Isoflavin beta, we evaluated the influence of the fermentation and drying process in the extracts efficacy. The results demonstrated that inlet gas temperature and adjuvant concentration for the extract drying process have significantly affected the total polyphenol contents and, to a minor degree, the genistein contents. However, the effect of topical stimulus with TPA, an oxidative stress inducer, which caused significant depletion of reduced glutathione (GSH) and catalase, with increased levels of H2O2 and lipid peroxidation (MDA) in the skin of hairless mice, was significantly prevented by the soybean extracts treatment. These results indicate that the spray drying processing resulted in a product capable of limiting the oxidative stress with possible therapeutic applicability as an antioxidant in pharmaceutical forms.
Department of Pharmaceutical Science, Health Sciences Center, Londrina State University (UEL), Avenue Robert Koch 60, 86038350 Londrina, PR, Brazil ; Department of Pharmaceutical Science, Faculty of Pharmaceutical Sciences of Ribeirao Preto (USP), Avenue Do Café s/n, 14040903 Ribeirao Preto, SP, Brazil.
Sandra R
SR
Georgetti
Rúbia
R
Casagrande
Fabiana T M C
FT
Vicentini
Marcela M
MM
Baracat
Waldiceu A
WA
Verri
Maria J V
MJ
Fonseca
D000818:Animals; D002374:Catalase / Q000378:metabolism; D003890:Desiccation; D005260:Female; D005285:Fermentation / Q000187:drug effects*; D019833:Genistein / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology; D020011:Protective Agents / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects* / Q000473:pathology*; D013025:Glycine max / Q000737:chemistry*; D013755:Tetradecanoylphorbol Acetate
D010936:Plant Extracts; D059808:Polyphenols; D020011:Protective Agents; D006861:Hydrogen Peroxide; D019833:Genistein; D002374:Catalase; D005978:Glutathione; D013755:Tetradecanoylphorbol Acetate