The long term oral regulation of blood glucose in diabetic patients by using of Escherichia coli Nissle 1917 expressing CTB-IGF-1 hybrid protein.
Regarding to the high prevalence and comorbidities of chronic high blood glucose in diabetic patients and the limited efficacy and current painful treatments. It is necessary to improve new treatments that are non-invasive and long-term for controlling blood glucose. Recent studies have shown that the healthy microflora in different body organs can perform as the gene vectors for expressing different types of gene therapies in situ. We have proposed that by constructing a recombinant Escherichia coli Nissle 1917 that expresses CTB-IGF-1 hybrid gene under control of ompC glucose sensitive promoter, the intestinal glucose level can be regulated. This method in comparison with other methods is a non-invasive way to control the blood glucose orally and it can be used for all types of diabetes.
Department of Biotechnology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Zahra
Z
Bazi
Mahsa
M
Jalili
Azita
A
Hekmatdoost
D000284:Administration, Oral; D001786:Blood Glucose / Q000187:drug effects*; D002352:Carrier Proteins / Q000378:metabolism / Q000494:pharmacology*; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy*; D004926:Escherichia coli / Q000378:metabolism*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism / Q000494:pharmacology*; D007422:Intestines / Q000382:microbiology; D064307:Microbiota / Q000235:genetics; D008954:Models, Biological; D018272:Porins / Q000235:genetics; D019936:Probiotics / Q000378:metabolism / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011509:Proteoglycans / Q000378:metabolism / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism / Q000494:pharmacology*
D001786:Blood Glucose; D002352:Carrier Proteins; C113724:OmpC protein; D018272:Porins; D011509:Proteoglycans; D011993:Recombinant Fusion Proteins; C069126:cytotactin-binding proteoglycan; D007334:Insulin-Like Growth Factor I