Vitellogenin from the silkworm, Bombyx mori: an effective anti-bacterial agent.
Silkworm, Bombyx mori, vitellogenin (Vg) was isolated from perivisceral fat body of day 3 of pupa. Both Vg subunits were co-purified as verified by mass spectrometry and immunoblot. Purified Vg responded to specific tests for major posttranslational modifications on native gels indicating its nature as lipo-glyco-phosphoprotein. The Vg fraction had strong antibacterial activity against Gram negative bacterium Escherichia coli and Gram positive bacterium Bacillus subtilis. Microscopic images showed binding of Vg to bacterial cells and their destruction. When infected silkworm larvae were treated with purified Vg they survived the full life cycle in contrast to untreated animals. This result showed that Vg has the ability to inhibit the proliferation of bacteria in the silkworm fluid system without disturbing the regular metabolism of the host.
Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli, India.
Nitin Kumar
NK
Singh
Britto Cathrin
BC
Pakkianathan
Manish
M
Kumar
Tulika
T
Prasad
Mani
M
Kannan
Simone
S
König
Muthukalingan
M
Krishnan
D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development; D012831:Bombyx / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005216:Fat Body / Q000737:chemistry / Q000378:metabolism; D007814:Larva / Q000187:drug effects / Q000382:microbiology; D050296:Microbial Viability / Q000187:drug effects; D010750:Phosphoproteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D011485:Protein Binding; D021122:Protein Subunits / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D011679:Pupa / Q000737:chemistry / Q000378:metabolism; D014819:Vitellogenins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D010750:Phosphoproteins; D021122:Protein Subunits; D014819:Vitellogenins