Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia.
Visualization of signal transduction in live primary cilia constitutes a technical challenge owing to the organelle's submicrometer dimensions and close proximity to the cell body. Using a genetically encoded calcium indicator targeted to primary cilia, we visualized calcium signaling in cilia of mouse fibroblasts and kidney cells upon chemical or mechanical stimulation with high specificity, high sensitivity and wide dynamic range.
1] Department of Biomedical Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, Maryland, USA. [2] Department of Cell Biology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA. [3] Center for Cell Dynamics, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA. [4].
Steven
S
Su
Siew Cheng
SC
Phua
Robert
R
DeRose
Shuhei
S
Chiba
Keishi
K
Narita
Peter N
PN
Kalugin
Toshiaki
T
Katada
Kenji
K
Kontani
Sen
S
Takeda
Takanari
T
Inoue
D000818:Animals; D020013:Calcium Signaling / Q000235:genetics*; D002923:Cilia / Q000378:metabolism*; D051379:Mice; D015398:Signal Transduction