Real-time in vivo imaging of surface-modified liposomes to evaluate their behavior after pulmonary administration.
Our previous study demonstrated that surface modification of liposomes using polyvinyl alcohol with a hydrophobic anchor (PVA-R) achieved sustained absorption from the lung after pulmonary administration and prolonged the pharmacological effects of the model peptide drug. In the present study, the behavior of PVA-R-modified liposomes in the lung and whole body was monitored using a real-time in vivo imaging system. Subsequently, the influence of surface modification with PVA-R on liposomal behavior in lung tissue was examined. Indocyanine green (ICG) was used as a near-infrared label of PVA-R-modified liposomes and was used to observe their dynamic behavior using non-invasive in vivo imaging (IVIS® imaging system) after pulmonary administration to rats. PVA-R-modified submicron-sized liposomes (ssLips) induced long-term retention in the lung compared with unmodified liposomes. Moreover, liposome association with alveolar macrophages (NR8383) was decreased by PVA-R modification in vitro. Therefore, PVA-R modification may prevent rapid elimination of ssLips by macrophages, thereby increasing retention in the lung.
Laboratory of Pharmaceutical Engineering, Gifu Pharmaceutical University, Gifu, Japan.
Mitsutaka
M
Murata
Laboratory of Pharmaceutical Engineering, Gifu Pharmaceutical University, Gifu, Japan.
Kohei
K
Tahara
Laboratory of Pharmaceutical Engineering, Gifu Pharmaceutical University, Gifu, Japan. Electronic address: takeuchi@gifu-pu.ac.jp.
Hirofumi
H
Takeuchi
D000818:Animals; D004396:Coloring Agents / Q000008:administration & dosage; D057927:Hydrophobic and Hydrophilic Interactions; D007208:Indocyanine Green / Q000008:administration & dosage; D008081:Liposomes / Q000737:chemistry / Q000493:pharmacokinetics; D008168:Lung / Q000378:metabolism*; D008297:Male; D057054:Molecular Imaging; D010316:Particle Size; D010455:Peptides / Q000008:administration & dosage / Q000493:pharmacokinetics; D010713:Phosphatidylcholines / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D019265:Spectroscopy, Near-Infrared; D013499:Surface Properties; D014018:Tissue Distribution
D004396:Coloring Agents; D008081:Liposomes; D010455:Peptides; D010713:Phosphatidylcholines; D011142:Polyvinyl Alcohol; C010942:1,2-distearoyllecithin; D007208:Indocyanine Green
IVIS®; In vivo imaging; Indocyanine green; Liposomal surface modification; PVA; Pulmonary drug delivery