Biomass composition, lipid characterization, and metabolic profile analysis of the fed-batch fermentation process of two different docosahexanoic acid producing Schizochytrium sp. strains.
Growth and fermentation characteristics, biomass composition, lipid characterization and metabolic profiling analysis of two different Schizochytrium sp. strains, the original strain and the industrial adaptive strain, were investigated in the fed-batch fermentation process. The final cell biomass, total lipids content, docosahexanoic acid (DHA) content and DHA productivity of the adaptive strain were much higher than those of the original strain. The metabolic distinctions which extensively existed between these two strains were revealed by the score plot of principal component analysis. In addition, potential biomarkers responsible for discriminating different strains were identified as myo-inositol, histidine, alanine, asparagine, cysteine, and oxalic acid. These findings provided new insights into the industrial strain screening and further improvement of DHA production by Schizochytrium sp.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing, 210009, People's Republic of China.
Liang
L
Qu
Lu-Jing
LJ
Ren
Juan
J
Li
Guan-Nan
GN
Sun
Li-Na
LN
Sun
Xiao-Jun
XJ
Ji
Zhi-Kui
ZK
Nie
He
H
Huang
D061249:Batch Cell Culture Techniques / Q000379:methods*; D018533:Biomass; D004281:Docosahexaenoic Acids / Q000096:biosynthesis*; D005285:Fermentation; D007221:Industry; D050356:Lipid Metabolism; D055442:Metabolome; D013045:Species Specificity; D058009:Stramenopiles / Q000254:growth & development* / Q000378:metabolism*; D013997:Time Factors
D004281:Docosahexaenoic Acids