Flavonoids from the grains of C1/R-S transgenic rice, the transgenic Oryza sativa spp. japonica, and their radical scavenging activities.
The transgenic rice cultivar of Oryza sativa spp. japonica cv. Hwa-Young, C1/R-S transgenic rice (C1/R-S rice), is a flavonoid-rich cultivar of rice. The grains of C1/R-S rice were extracted with aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH, and H2O, successively. Repeated silica gel, octadecyl silica gel (ODS), and Sephadex LH-20 column chromatographies for the EtOAc and n-BuOH fractions afforded four new flavonoids (compounds 2, 3, 7, and 8) along with four known flavonoids: (+)-3'-O-methyltaxifolin (1), brassicin (4), isorhamnetin-4'-O-β-D-glucosyranoside (5), and 3'-O-methyltaxifolin-5-O-β-D-glucopyranoside (6). The new flavonoids were identified as 3'-O-methyltaxifolin-7-O-β-D-glucopyranoside (2), 3'-O-methyltaxifolin-4'-O-β-D-glucopyranoside (3), isorhamnetin-7-O-β-D-cellobioside (brassicin-4″-O-β-D-glucopyranoside) (7), and brassicin-4'-O-β-D-glucosyranoside (8) from the result of spectroscopic data including nuclear magnetic resonance spectrometry (NMR), mass spectrometry (MS), and infrared spectroscopy (IR). Also, quantitative analysis of major flavonoids (compounds 2, 3, and 8) in C1/R-S rice, O. sativa spp. japonica cv. Hwa-Young (HY), and a hybrid of two cultivar (C1/R-S rice/HY) extracts was performed using HPLC experiment. The isolated flavonoids were evaluated for their radical-scavenging effect on DPPH and ABTS radicals.
Graduate School of Biotechnology, Institute of Life Sciences and Resources, Kyung Hee University , Yongin 446-701, Republic of Korea.
Jin-Gyeong
JG
Cho
Na-Young
NY
Song
Tae-Gyu
TG
Nam
Sabina
S
Shrestha
Hee-Jung
HJ
Park
Ha-Na
HN
Lyu
Dae-Ok
DO
Kim
Gihwan
G
Lee
Young-Min
YM
Woo
Tae-Sook
TS
Jeong
Nam-In
NI
Baek
D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis* / Q000378:metabolism; D016166:Free Radical Scavengers / Q000032:analysis* / Q000378:metabolism; D012275:Oryza / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000032:analysis* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D005419:Flavonoids; D016166:Free Radical Scavengers; D010936:Plant Extracts