Study on the simultaneous determination of seven benzoylurea pesticides in Oolong tea and their leaching characteristics during infusing process by HPLC-MS/MS.
A method for the simultaneous determination of 7 benzoylurea pesticides (chlorfluazuron, diflubenzuron, fluazuron, flufenoxuron, hexaflumuron, teflubenzuron and triflumuron) in the manufactured Oolong tea leaves and its infusion was described. The method has a LOD of 0.03-1.00ng/mL, a recovery of 90.4-103% for made tea and 90.3-102% for tea-infused liquid, respectively. By using the proposed method, the leaching characteristics of above 7 pesticides during infusing process were investigated. The experimental results revealed that: (1) diflubenzuron can be most easily extracted out during infusing process, followed by triflumuron, teflubenzuron, hexaflumuron, chlorfluazuron, flufenoxuron and fluazuron. (2) The leaching of flufenoxuron and chlorfluazuron during infusing process seems to be controlled by only their solubility, whereas, the leaching of other 5 benzoylurea insecticides was primarily controlled by their partitioning coefficient between made tea and hot water. The results of this study are helpful for the accurate evaluation of the safety of Oolong tea.
Key Lab of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China; National Tea Quality Supervision and Inspection Center (Fujian)/Quanzhou Quality Inspection Institute of Product, Quanzhou, Fujian, 362000, China.
Lei
L
Chen
JinFa
J
Chen
Ying
Y
Guo
JianRong
J
Li
YiQiang
Y
Yang
LiangJun
L
Xu
FengFu
F
Fu
D028241:Camellia sinensis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010573:Pesticide Residues / Q000737:chemistry*; D010671:Phenylurea Compounds / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013662:Tea / Q000737:chemistry*
D010573:Pesticide Residues; D010671:Phenylurea Compounds; D013662:Tea
Benzoylurea pesticides; HPLC–MS/MS; Infusion of tea; Oolong tea; Solid phase extraction