Caspase-mediated cleavage and DNase activity of the translation initiation factor 3, subunit G (eIF3g).
Eukaryotic translation initiation factor 3 is composed of 13 subunits (eIF3a through eIF3m) and plays an essential role in translation. During apoptosis, several caspases rapidly down-regulate protein synthesis by cleaving eIF4G, -4B, -3j, and -2α. In this study, we found that the activation of caspases by cisplatin in T24 cells induces the cleavage of subunit G of the eIF3 complex (eIF3g). The cleavage site (SLRD(220)G) was identified, and we found that the cleaved N-terminus was translocated to the nucleus, activating caspase-3, and that it also showed a strong DNase activity. These data demonstrate the important roles of eIF3g in the translation initiation machinery and in DNA degradation during apoptosis.
Medical Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
Jong-Tae
JT
Kim
Seon-Jin
SJ
Lee
Bo-Yeon
BY
Kim
Chul-Ho
CH
Lee
Young Il
YI
Yeom
Yong-Kyung
YK
Choe
Do-Young
DY
Yoon
Suhn-Kee
SK
Chae
Jung Woo
JW
Kim
Young
Y
Yang
Jong-Seok
JS
Lim
Hee Gu
HG
Lee
D021581:Active Transport, Cell Nucleus; D020816:Amino Acid Motifs; D053148:Caspase 3 / Q000378:metabolism*; D053179:Caspase 7 / Q000378:metabolism*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D053837:DNA Cleavage; D003851:Deoxyribonucleases / Q000378:metabolism*; D004789:Enzyme Activation; D039621:Eukaryotic Initiation Factor-3 / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D010446:Peptide Fragments / Q000378:metabolism; D059748:Proteolysis
D039621:Eukaryotic Initiation Factor-3; D010446:Peptide Fragments; D003851:Deoxyribonucleases; C505407:CASP3 protein, human; C505520:CASP7 protein, human; D053148:Caspase 3; D053179:Caspase 7
Apoptosis; Caspase; DNase; Translation initiation factor; eIF3g