Phenylboronic acid-functionalized polymeric micelles with a HepG2 cell targetability.
Phenylboronic acid-functionalized amphiphilic block copolymer Pluronic-PMCC-BA was synthesized via ring-opening polymerization of 5-methyl-5-benzyloxycarbonyl-1,3-dioxan-2-one (MBC) with fumaric acid as a catalyst followed by the deprotection of carboxyl groups by catalyzed hydrogenation and the condensation of 3-aminophenylboronic acid with the copolymer side groups. Pluronic-PMCC-BA can form stable micelle solution by self-assembly in water. The phenylboronic acid groups are located at the shell of micelle as proved by (1)H NMR. The diameter of drug-free micelles is approximate 60 nm. Nano-spheres with narrow size distribution could be observed in the TEM image. MTT assay results show that Pluronic-PMCC-BA exhibits slight cytotoxicity when the polymer concentration is higher than 25 μg mL(-1). The toxicities of DOX@Pluronic-PMCC and DOX@Pluronic-PMCC-BA to COS7, HeLa, and HepG2 cell lines are similar with those of free DOX. Interestingly, phenylboronic acid groups located at the surface of Pluronic-PMCC-BA micelles can recognize HepG2 cells and promote the drug uptake of the cells, which are observed by confocal laser scanning microscopy (CLSM). The results imply that Pluronic-PMCC-BA would be a promising material for targeted drug delivery to the cancer cells.
Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Xiaojin
X
Zhang
Zhenguo
Z
Zhang
Xin
X
Su
Mengmeng
M
Cai
Renxi
R
Zhuo
Zhenlin
Z
Zhong
D001897:Boronic Acids / Q000737:chemistry*; D016503:Drug Delivery Systems; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D011108:Polymers / Q000009:adverse effects / Q000737:chemistry*
D001897:Boronic Acids; D008823:Micelles; D011108:Polymers; C010686:benzeneboronic acid
Controlled drug release; Copolymer; Pluronics; Polycarbonate; Polymeric micelle