A simple method for the determination of the cholesterol esterase activity.
The proposed method determines the activity of cholesterol esterase (CEH) and takes advantage of its ability to catalyze the hydrolysis of cholesterol esters naturally present in human serum. The assay is based on Allain's method of spectrophotometric determination of cholesterol by means of cholesterol oxidase, peroxidase, but using 3,5-dichloro-dihydroxybenzenesulfonic acid (DHBS) as phenolic chromogen and human serum as a source of substrate for the CEH as a novelty. Furthermore, it is characterized by low costs and high precision. It can be employed to control the activity of CE preparations used for the preparation of enzymatic kits for the determination of cholesterol or for screening of potential bacterial enzyme producers.
Department of Biotechnology, Institute of Applied Biotechnology and Basic Science; and Centre of Applied Biotechnology and Basic Sciences, University of Rzeszow, Kolbuszowa, Poland.
Agnieszka Ewa
AE
Stępień
Mykhailo
M
Gonchar
D001557:Benzenesulfonates / Q000737:chemistry; D001798:Blood Proteins / Q000737:chemistry*; D002784:Cholesterol / Q000097:blood*; D002788:Cholesterol Esters / Q000097:blood*; D002789:Cholesterol Oxidase / Q000737:chemistry; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D009195:Peroxidase / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*; D002787:Sterol Esterase / Q000737:chemistry*
D001557:Benzenesulfonates; D001798:Blood Proteins; D002788:Cholesterol Esters; D002784:Cholesterol; D002789:Cholesterol Oxidase; D009195:Peroxidase; D002787:Sterol Esterase