Synergistic effect of proanthocyanidin on the bactericidal action of the photolysis of H2O2.
The in vitro antibacterial activity of the hydroxyl radical generation system by the photolysis of H2O2 in combination with proanthocyanidin, which refers to a group of polyphenolic compounds, was examined. Bactericidal activity of photo-irradiated H2O2 at 405 nm against Streptococcus mutans, a major pathogen of dental caries, was augmented in the presence of proanthocyanidin, whose bactericidal effect by itself was very poor, in a concentration-dependent manner. This combination was also proven effective against Porphyromonas gingivalis, a major pathogen of periodontitis. It is speculated that H2O2, generated from photo-irradiated proanthocyanidin around the bacterial cells, is photolyzed to the hydroxyl radical, which would in turn affect the membrane structure and function of the bacterial cells, resulting in augmented sensitivity of bacterial cells to the disinfection system utilizing the photolysis of H2O2. The present study suggests that the combination of H2O2 and proanthocyanidin works synergistically to kill bacteria when photo-irradiated.
Tohoku University Graduate School of Dentistry.
Hiroyo
H
Ikai
Keisuke
K
Nakamura
Taro
T
Kanno
Midori
M
Shirato
Luiz
L
Meirelles
Keiichi
K
Sasaki
Yoshimi
Y
Niwano
D000900:Anti-Bacterial Agents / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects; D004357:Drug Synergism; D006861:Hydrogen Peroxide / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000378:metabolism; D050296:Microbial Viability / Q000187:drug effects*; D010782:Photolysis; D016966:Porphyromonas gingivalis / Q000187:drug effects; D044945:Proanthocyanidins / Q000378:metabolism*; D013295:Streptococcus mutans / Q000187:drug effects
D000900:Anti-Bacterial Agents; D044945:Proanthocyanidins; C013221:proanthocyanidin; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide