Phenolic biotransformations during conversion of ferulic acid to vanillin by lactic acid bacteria.
Vanillin is widely used as food additive and as a masking agent in various pharmaceutical formulations. Ferulic acid is an important precursor of vanillin that is available in abundance in cell walls of cereals like wheat, corn, and rice. Phenolic biotransformations can occur during growth of lactic acid bacteria (LAB), and their production can be made feasible using specialized LAB strains that have been reported to produce ferulic acid esterases. The present study aimed at screening a panel of LAB isolates for their ability to release phenolics from agrowaste materials like rice bran and their biotransformation to industrially important compounds such as ferulic acid, 4-ethyl phenol, vanillic acid, vanillin, and vanillyl alcohol. Bacterial isolates were evaluated using ferulic acid esterase, ferulic acid decarboxylase, and vanillin dehydrogenase assays. This work highlights the importance of lactic acid bacteria in phenolic biotransformations for the development of food grade flavours and additives.
Department of Biotechnology, Punjabi University, Patiala 147002, India.
Baljinder
B
Kaur
Debkumar
D
Chakraborty
Balvir
B
Kumar
D000445:Aldehyde Oxidoreductases / Q000737:chemistry / Q000378:metabolism; D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D001547:Benzaldehydes / Q000737:chemistry*; D001592:Benzyl Alcohols / Q000737:chemistry / Q000378:metabolism; D001711:Biotransformation; D002262:Carboxy-Lyases / Q000737:chemistry / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000378:metabolism; D003373:Coumaric Acids / Q000737:chemistry*; D019344:Lactic Acid / Q000378:metabolism; D010636:Phenols / Q000737:chemistry / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry
D001547:Benzaldehydes; D001592:Benzyl Alcohols; D003373:Coumaric Acids; D010636:Phenols; D019344:Lactic Acid; C042291:4-ethylphenol; C004999:ferulic acid; C100058:vanillin; D000445:Aldehyde Oxidoreductases; C105452:vanillin dehydrogenase; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; D002262:Carboxy-Lyases; C087194:phenylacrylic acid decarboxylase; C024078:vanillyl alcohol