[Effect of PC cell-derived growth factor RNA interference on biological behavior of esophageal squamous carcinoma cells].
OBJECTIVE
To investigate the effect of PC cell-derived growth factor (PCDGF) RNA interference on esophageal squamous carcinoma cells Eca-109 in vitro.


METHODS
The PCDGF-shRNA expression vector was transfected into the Eca-109 cells by liposome. After transfection, the mRNA and protein expressions of PCDGF were detected by RT-PCR and Western-blot respectively. Cell Counting Kit-8 (CCK-8) assay and Boyden chamber method were performed to measure the cell proliferation and invasion ability respectively.


RESULTS
The expression levels of PCDGF mRNA and protein were both decreased in Eca-109 cells transfected with PCDGF-shRNA expression vector (transfection group). Twenty-four, 48 and 72 h after transfection, the cells proliferation in the transfection group was inhibited, and the inhibition rate was 20.4%, 21.1% and 20.9% respectively. The cell proliferation activity in the transfection group was significantly lower than that in the non-transfection group, liposome group and negative vector group (all P<0.05). The number of cell migration in the non-transfection group,negative vector group, liposome group and transfection group was 118.8±12.0, 100.8±9.0, 114.3±4.7, and 53.5±16.3 respectively. The differences were statistically significant between the transfection group and the other 3 groups (all P<0.05).


CONCLUSIONS
PCDGF RNA interference can inhibit the proliferation and invasion abilities of esophageal squamous carcinoma cells in vitro. PCDGF gene may be the new target of gene therapy.
Department of Thoracic Surgery, Teaching Hospital of Fujian Medical University, Fujian Provincial Cancer Hospital, Fujian Provincial Key Laboratory of Tumor Biotherapy, Fuzhou 350014, China. zhqf@msn.com.
Qing-feng
QF
Zheng
Shuo-yan
SY
Liu
Hai-yan
HY
Wang
Feng
F
Wang
Zhen
Z
Wang
Xiao-feng
XF
Chen
Jian-jian
JJ
Wang
Min-gang
MG
Ying
Xiong-wei
XW
Zheng
Xian-dong
XD
Lin
Zhi-feng
ZF
Zhou
Fu-sheng
FS
Gong
Yun-qing
YQ
Xie
D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000473:pathology*; D000077277:Esophageal Squamous Cell Carcinoma; D005822:Genetic Vectors; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D000077153:Progranulins; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection
C492314:GRN protein, human; D036341:Intercellular Signaling Peptides and Proteins; D000077153:Progranulins; D034741:RNA, Small Interfering