Genetic variants in BMP8B gene are associated with growth traits in Chinese native cattle.
As a signaling molecule, bone morphogenetic protein 8B (BMP8B) plays an essential role in bone metabolism and is able to regulate thermogenesis and energy balance, which suggests that BMP8B gene may be a new candidate for growth traits. Here, to characterize the effects of BMP8B gene on growth traits, we first used three Chinese indigenous cattle breeds (n=845) to detect single nucleotide polymorphisms (SNPs). Five novel SNPs of BMP8B gene (g.-242C>T, g.2164C>T, g.2639T>C, g.2900C>G and g.10817C>T) were identified by DNA pool sequencing and forced PCR-RFLP. And then we associated the five SNPs with four growth traits (body weight, body length, heart girth, and hucklebone width). Results from association analysis showed that the SNPs 1, 2, and 3 affected growth trait(s) markedly (P<0.05). Further, 6 combined haplotypes were constructed to guarantee the reliability of analysis results. There were also significant differences in body length, heart girth and body weight between the 6 combined haplotypes (P<0.05), but not in hucklebone width (P>0.05). Collectively, our results suggest a modulatory role of BMP8B gene in cattle growth and development, and 3 SNPs could be used as molecular markers in early marker assisted selection (MAS) in beef cattle breeding program.
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
Xiu-Kai
XK
Cao
Jing
J
Wang
Xian-Yong
XY
Lan
Chu-Zhao
CZ
Lei
Chun-Lei
CL
Zhang
Xing-Lei
XL
Qi
Hong
H
Chen
D000818:Animals; D001835:Body Weight / Q000235:genetics; D019485:Bone Morphogenetic Proteins / Q000235:genetics*; D001947:Breeding; D002417:Cattle / Q000235:genetics* / Q000254:growth & development*; D002681:China; D014644:Genetic Variation; D006239:Haplotypes; D015810:Linkage Disequilibrium; D020641:Polymorphism, Single Nucleotide; D019655:Quantitative Trait, Heritable
D019485:Bone Morphogenetic Proteins
Asp; BAT; BMP8B gene; BMPs; Cattle; Combined haplotype; GLM; General Linear Model; Growth traits; HWE; Hap; Hardy–Weinberg equilibrium; He; Ho; JX; Jiaxian cattle; LD; LE; MAS; NY; Nanyang cattle; Ne; PCR–RFLP; PGC; PIC; QC; Qinchuan cattle; SE; SNP; SNP(s); TBE; TGF-β; a buffer solution containing a mixture of Tris base, boric acid and EDTA; aspartic acid; base pair(s); bone morphogenetic proteins; bp; brown adipose tissue; effective allele numbers; haplotype; linkage disequilibrium; linkage equilibrium; marker assisted selection; observed heterozygosity; observed homozygosity; polymerase chain reaction–restricted fragment length polymorphisms; polymorphism information content; primordial germ cell; single nucleotide polymorphism(s); standard error; transforming growth factor-beta