Magnetic molecularly imprinted polymer for the selective extraction of sildenafil, vardenafil and their analogs from herbal medicines.
The successfully developed magnetic molecularly imprinted polymers (MMIPs) toward six synthetic phosphodiesterase type-5 (PDE-5) inhibitors were described. Sildenafil was used as template for the preparation of MMIPs using superparamagnetic core-shell nanoparticle as supporter. The obtained MMIPs were characterized using transmission electron microscope, Fourier transform infrared, X-ray diffraction, and vibrating sample magnetometer. High performance liquid chromatography (HPLC) with diode array detector (DAD) was used for the analysis of target analytes. The application of MMIPs as selective sorbent in the cleanup of herbal medicine samples prior to HPLC offered simple sample preparation. The adsorption capacity and selectivity of prepared MMIPs and magnetic non-molecularly imprinted polymers were investigated. The binding isotherms were obtained for sildenafil and fitted by Freundlich isotherm model. Structurally similar compound of sildenafil and a reference compound protocatechuic acid were used for investing the selective recognition of MMIPs.
Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China; Graduate University of Chinese Academy of Sciences, Beijing 100039, PR China.
Fang-Fang
FF
Chen
Xiao-Yu
XY
Xie
Yan-Ping
YP
Shi
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D062385:Hydroxybenzoates / Q000737:chemistry; D007093:Imidazoles / Q000737:chemistry / Q000302:isolation & purification*; D058185:Magnetite Nanoparticles / Q000737:chemistry; D060306:Magnetometry; D059346:Magnets; D046529:Microscopy, Electron, Transmission; D054802:Molecular Imprinting; D058986:Phosphodiesterase 5 Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D010879:Piperazines / Q000737:chemistry / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*; D011687:Purines / Q000737:chemistry / Q000302:isolation & purification; D000068677:Sildenafil Citrate; D052616:Solid Phase Extraction; D017550:Spectroscopy, Fourier Transform Infrared; D013450:Sulfones / Q000737:chemistry / Q000302:isolation & purification*; D014227:Triazines / Q000737:chemistry / Q000302:isolation & purification; D000069058:Vardenafil Dihydrochloride
D004365:Drugs, Chinese Herbal; D062385:Hydroxybenzoates; D007093:Imidazoles; D058185:Magnetite Nanoparticles; D058986:Phosphodiesterase 5 Inhibitors; D010879:Piperazines; D011687:Purines; D013450:Sulfones; D014227:Triazines; C009091:protocatechuic acid; D000069058:Vardenafil Dihydrochloride; D000068677:Sildenafil Citrate
Herbal medicines; Magnetic molecularly imprinted polymers; Phosphodiesterase type-5 inhibitors; Sildenafil; Solid phase extraction