PNA-FISH assays for early targeted bacteraemia treatment.
PNA-FISH S. aureus/CNS and GNR Traffic Light assays were compared with standard culture methods for identifying bacteraemia in 156 blood cultures from 131 patients. Results correlated with final culture results in 153 cultures. Retrospective case note review revealed that earlier targeted treatment would have occurred in 10.7% of cases.
Department of Medical Microbiology, Ninewells Hospital and Medical School, NHS Tayside, Dundee, United Kingdom. Electronic address: b.parcell@nhs.net.
B J
BJ
Parcell
G V
GV
Orange
D016470:Bacteremia / Q000175:diagnosis* / Q000628:therapy; D004926:Escherichia coli / Q000302:isolation & purification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007711:Klebsiella pneumoniae / Q000302:isolation & purification; D020135:Peptide Nucleic Acids; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000302:isolation & purification*
D020135:Peptide Nucleic Acids
API; Analytical profile index; Bacteraemia; Blood cultures; CLSI; Clinical and Laboratory Standards Institute; GNR; GPC; Gram negative rods; Gram positive cocci; MALDI-TOF MS; MRSA; MSSA; Matrix-assisted laser desorption/ionisation-time of flight mass spectrometry; Meticillin-resistant Staphylococcus aureus; Meticillin-sensitive Staphylococcus aureus; PCR; PNA-FISH; Peptide nucleic acid fluorescence in situ hybridization; Polymerase chain reaction; Treatment