Hepatoprotective function of Penthorum chinense Pursh.
This research was to figure out the hepatoprotective constituents of Penthorum chinense Pursh, a typical species both for food and medicine, using carbon tetrachloride-induced chronic hepatotoxicity in HL-7702 cells. Cell viability, levels of microsomal enzymes, alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and the concentration of malondialdehyde (MDA) were determined in homogenates of the liver cells. Results showed that the cell viability increased significantly in all test groups in a concentration-dependent manner and the content of the peroxidation product MDA decreased significantly, similar to the serum levels of hepatic enzyme biomarkers (ALT and AST). The effects of 70% ethanol extracts and Vc were better than 95% ethanol and water extracts, and the ethyl acetate extracts further obtained from 70% ethanol fraction showed the highest hepatoprotective activity, even better than Vc. The ethyl acetate fraction from 70% ethanol extracts is responsible for the hepatoprotective function of P. chinense.
College of Food and Bioengineering, South China University of Technology, Guangzhou, 510640, China. jgjiang@scut.edu.cn.
Tian-Tian
TT
Zhang
Xi-Lin
XL
Xu
Ming-Hua
MH
Jiang
Jian-Guo
JG
Jiang
D000410:Alanine Transaminase / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D019684:Magnoliopsida / Q000737:chemistry*; D008315:Malondialdehyde / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*
D010936:Plant Extracts; D020011:Protective Agents; D008315:Malondialdehyde; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase