Rifampin heteroresistance in Mycobacterium tuberculosis cultures as detected by phenotypic and genotypic drug susceptibility test methods.
Tuberculosis patients may harbor both drug-susceptible and -resistant bacteria, i.e., heteroresistance. We used mixtures of rifampin-resistant and -susceptible Mycobacterium tuberculosis strains to simulate heteroresistance in patient samples. Molecular tests can be used for earlier discovery of multidrug resistance (MDR), but the sensitivity to detect heteroresistance is unknown. Conventional phenotypic drug susceptibility testing was the most sensitive, whereas two line probe assays and sequencing were unable to detect the clinically important 1% resistant bacteria.
International Reference Laboratory of Mycobacteriology, Statens Serum Institut, Copenhagen, Denmark.
Dorte Bek
DB
Folkvardsen
Vibeke Ø
VØ
Thomsen
Leen
L
Rigouts
Erik Michael
EM
Rasmussen
Didi
D
Bang
Gertjan
G
Bernaerts
Jim
J
Werngren
Juan Carlos
JC
Toro
Sven
S
Hoffner
Doris
D
Hillemann
Erik
E
Svensson
D000995:Antitubercular Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics; D010641:Phenotype; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity
D000995:Antitubercular Agents; D012293:Rifampin