The crystal structure of HIV CRF07 B'/C gp41 reveals a hyper-mutant site in the middle of HR2 heptad repeat.
HIV CRF07 B'/C is a strain circulating mainly in northwest region of China. The gp41 region of CRF07 is derived from a clade C virus. In order to compare the difference of CRF07 gp41 with that of typical clade B virus, we solved the crystal structure of the core region of CRF07 gp41. Compared with clade B gp41, CRF07 gp41 evolved more basic and hydrophilic residues on its helix bundle surface. Based on sequence alignment, a hyper-mutant cluster located in the middle of HR2 heptads repeat was identified. The mutational study of these residues revealed that this site is important in HIV mediated cell-cell fusion and plays critical roles in conformational changes during viral invasion.
State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300071, China.
Jiansen
J
Du
Hailing
H
Xue
Jing
J
Ma
Fang
F
Liu
Jianhua
J
Zhou
Yiming
Y
Shao
Wentao
W
Qiao
Xinqi
X
Liu
D000596:Amino Acids / Q000235:genetics; D002459:Cell Fusion; D002681:China; D018360:Crystallography, X-Ray; D004252:DNA Mutational Analysis; D015700:HIV Envelope Protein gp41 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D008958:Models, Molecular; D009154:Mutation; D011487:Protein Conformation; D016415:Sequence Alignment; D053586:Virus Internalization
D000596:Amino Acids; D015700:HIV Envelope Protein gp41; C514787:gp41 protein, Human immunodeficiency virus 1
Crystal structure; HIV; Heptad repeat; Membrane fusion; gp41