Time to be versatile: regulation of the replication timing program in budding yeast.
Eukaryotic replication origins are activated at different times during the S phase of the cell cycle, following a temporal program that is stably transmitted to daughter cells. Although the mechanisms that control initiation at the level of individual origins are now well understood, much less is known on how cells coordinate replication at hundreds of origins distributed on the chromosomes. In this review, we discuss recent advances shedding new light on how this complex process is regulated in the budding yeast Saccharomyces cerevisiae. The picture that emerges from these studies is that replication timing is regulated in cis by mechanisms modulating the chromatin structure and the subnuclear organization of origins. These mechanisms do not affect the licensing of replication origins but determine their ability to compete for limiting initiation factors, which are recycled from early to late origins throughout the length of the S phase.
Institute of Human Genetics, CNRS UPR 1142, 141 rue de la Cardonille, Equipe Labellisée Ligue Contre le Cancer, 34396 Montpellier cedex 5, France; Department of Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Kazumasa
K
Yoshida
Ana
A
Poveda
Philippe
P
Pasero
D002875:Chromosomes; D004261:DNA Replication; D042522:DNA Replication Timing; D018741:Replication Origin; D012093:Replicon; D016196:S Phase; D004718:Saccharomycetales / Q000235:genetics* / Q000502:physiology
2D; ACS; ARS; ARS consensus sequence; BrdU; HDAC; ORC; SIR; autonomously replicating sequence; bromodeoxyuridine; checkpoints; histone deacetylase; histone deacetylases; initiation of DNA replication; origin recognition complex; pre-RC; pre-replication complex; replication foci; silent information regulator; two-dimensional