Two new stereoisomeric antioxidant triterpenes from Potentilla fulgens.
The roots of Potentilla fulgens have been used for a long time as a folk remedy for many ailments without having information on its pharmacological action. Of the various extracts prepared by partitioning of the methanol extract, the ethyl acetate fraction was found to possess better antioxidant and cytotoxic activities. The degree of reduction in cloning efficiencies of MCF-7 cell lines was more with ethyl acetate than with hexane fraction of the root-extract. Hence, this fraction was further purified and nine compounds, including two new ursane type triterpenoids Fulgic acid A (4) and Fulgic acid B (5), were identified and characterized. Other compounds were identified as ursolic acid, euscaphic acid, corosolic acid, epicatechin, catechin, p-hydroxybenzaldehyde and gallic acid. Chemical structures were elucidated by spectroscopic methods, especially ESIHRMS and 2D NMR techniques. The new compounds showed good antioxidant activity and therefore this plant can be a source of natural antioxidants.
Department of Natural Products, National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062 Punjab, India.
Alka
A
Choudhary
Department of Pharmaceutical Technology Biotechnology, National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062 Punjab, India.
Amit Kumar
AK
Mittal
Department of Biotechnology and Bioinformatics, North Eastern Hill University, Shillong, 793002 Meghalaya, India.
Manukonda
M
Radhika
Department of Biotechnology and Bioinformatics, North Eastern Hill University, Shillong, 793002 Meghalaya, India.
Debabrata
D
Tripathy
Department of Biotechnology and Bioinformatics, North Eastern Hill University, Shillong, 793002 Meghalaya, India.
Anupam
A
Chatterjee
Department of Pharmaceutical Technology Biotechnology, National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062 Punjab, India.
Uttam Chand
UC
Banerjee
Department of Natural Products, National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062 Punjab, India. Electronic address: ipsingh@niper.ac.in.
Inder Pal
IP
Singh
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001547:Benzaldehydes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002392:Catechin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005707:Gallic Acid / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D061986:MCF-7 Cells; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D031987:Potentilla / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D001547:Benzaldehydes; D010936:Plant Extracts; D014315:Triterpenes; C000588657:fulgic acid A; C000588658:fulgic acid B; D005707:Gallic Acid; D002392:Catechin; C011483:4-hydroxybenzaldehyde
Antioxidants; Fulgic acids; Potentilla fulgens; Rosaceae; Triterpenes; Ursane