Proteomic study of 'Moncada' mandarin buds from on- versus off-crop trees.
A proteomic analysis of buds from mandarin trees with contrasting fruit load (on- and off-crop trees) was carried out during the onset of low-temperature induction. The aim of the study was to find out more about the molecular mechanism relating to alternate bearing in Citrus and its relationship with flowering. The 'Moncada' variety (Clementine 'Oroval'x'Kara' mandarin), displaying remarkable behaviour in alternate production, was used in this study. From 2D DIGE gel, 192 spots were isolated: 97 showed increased expression in the off-crop buds as compared to the on-crop buds, while 95 exhibited enhanced expression in the on-crop buds versus the off-crop buds. These spots were identified by MALDI-MS or LC-MS-MS. The largest groups of proteins up-expressed in the off-crop buds were the proteins involved in carbohydrate and amino acid metabolism, and the proteins expressed in response to stimuli such as reactive oxygen species. The largest groups of proteins up-expressed in the on-crop buds were related to primary metabolism, oxidative stress and defence responses. Depending on their function, some of these proteins can stimulate the flowering, such as fructose-bisphosphate aldolase or leucine-rich repeat transmembrane protein kinase, while others can inhibit it, such as cytochrome c oxidase subunit II. Twenty-two other proteins with unknown functions were up-expressed in the on- or off-crop buds.
Instituto Agroforestal Mediterráneo, Universidad Politécnica de Valencia, C/Ingeniero Fausto Elio, E-46022 Valencia, Spain.
Natalia
N
Muñoz-Fambuena
Carlos
C
Mesejo
Carmina
C
Reig
Manuel
M
Agustí
Susana
S
Tárraga
Purificación
P
Lisón
Domingo J
DJ
Iglesias
Eduardo
E
Primo-Millo
M Carmen
MC
González-Mas
D000596:Amino Acids / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002957:Citrus / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D018556:Crops, Agricultural / Q000378:metabolism; D060467:Disease Resistance; D015180:Electrophoresis, Gel, Two-Dimensional; D035264:Flowers / Q000254:growth & development / Q000378:metabolism*; D005638:Fruit / Q000254:growth & development*; D015870:Gene Expression; D018519:Meristem / Q000378:metabolism*; D018384:Oxidative Stress; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014197:Trees / Q000378:metabolism
D000596:Amino Acids; D010940:Plant Proteins; D020543:Proteome
2D DIGE; 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulphonate; Alternate bearing; Amino acid metabolism; Buds; CHAPS; Carbohydrate metabolism; Citrus; DTT; IPG; LC–MS–MS; MALDI-MS; Ontology analysis; PMSF; Proteome; Q-TOF; SDS-PAGE; dithiothreitol; immobilised pH gradient; isoelectric point; liquid chromatography coupled with tandem mass spectrometry; matrix-assisted laser desorption/ionisation-mass spectrometry; pI; phenylmethylsulphonyl fluoride; quadrupole time-of-flight mass spectrometer; sodium dodecyl sulphate-polyacrylamide gel electrophoresis; two-dimensional difference gel electrophoresis