Contrasting quiescent G0 phase with mitotic cell cycling in the mouse immune system.
A transgenic mouse line expressing Fucci (fluorescent ubiquitination-based cell-cycle indicator) probes allows us to monitor the cell cycle in the hematopoietic system. Two populations with high and low intensities of Fucci signals for Cdt1(30/120) accumulation were identified by FACS analysis, and these correspond to quiescent G0 and cycling G1 cells, respectively. We observed the transition of immune cells between quiescent and proliferative phases in lymphoid organs during differentiation and immune responses.
Laboratory for Autoimmune Regulation, Research Center for Allergy and Immunology, RIKEN, Yokohama City, Kanagawa, Japan ; Laboratory for Cell Function and Dynamics, Brain Science Institute, RIKEN, Wako City, Saitama, Japan ; Center for Innovation in Immunoregulative Technology and Therapeutics, Kyoto University Graduate School of Medicine, Kyoto City, Japan.
Michio
M
Tomura
Asako
A
Sakaue-Sawano
Yoshiko
Y
Mori
Mitsuyo
M
Takase-Utsugi
Akihiro
A
Hata
Kenji
K
Ohtawa
Osami
O
Kanagawa
Atsushi
A
Miyawaki
D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D016193:G1 Phase / Q000502:physiology; D006413:Hematopoietic System / Q000166:cytology*; D007107:Immune System / Q000166:cytology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D016192:Resting Phase, Cell Cycle / Q000502:physiology*