Comparison of three indirect calorimetry devices and three methods of gas collection: a prospective observational study.
OBJECTIVE
Indirect calorimetry was performed for a long time with the DeltatracII(®) device (Datex, Finland), considered as a reference but no longer produced. This study aims at comparing the energy expenditure (EE), the volume of oxygen (VO2) and carbon dioxide (VCO2) measured by two new available indirect calorimeters, the QuarkRMR(®) (Cosmed, Italy) and the CCMexpress(®) (MedGraphic,USA), using three different methods of gas collection, with the DeltatracII(®) in healthy subjects.


METHODS
Twenty-four healthy subjects (15 women and 9 men, age 53 ± 15 yrs, mean BMI 25.5 ± 7.1 kg/m(2)) underwent measurements of EE with DeltatracII(®) using canopy, QuarkRMR(®) using canopy and CCMexpress(®) using canopy, face tent and facemask. All measurements were performed for 10 min in random order. Results are presented as mean ± SD and compared by linear regression, repeated measure one-way ANOVA with Bonferroni's post hoc test and Bland & Altman test.


RESULTS
EE was 1630 ± 340 kcal for DeltatracII(®) and 1607 ± 307 kcal, 1741 ± 360 kcal, 1666 ± 315 and 1626 ± 336 kcal for QuarkRMR(®) and CCMexpress(®) with canopy, face tent and facemask, respectively (p = 0.001). Compared to DeltatracII(®), Bland & Altman test showed a mean EE difference (2SD) of 24(220)kcal for QuarkRMR(®), and -111(260) kcal, -36(304) kcal, 5(402) kcal for CCMexpress(®) with canopy, face tent and facemask, respectively. There was no systematic over- or underestimation with any device or gas collection method.


CONCLUSIONS
Mean EE was similar between QuarkRMR(®) and DeltatracII(®) but not between CCMexpress(®), in any mode of gas collection, and DeltatracII(®). Bland & Altman test shows a large variability in EE differences with both devices compared to DeltatracII(®), highlighting the need for refining their accuracy.
Clinical Nutrition Unit, University Hospital, Geneva, Switzerland. Electronic address: severine.graf@hcuge.ch.
Séverine
S
Graf
Véronique Laurence
VL
Karsegard
Valérie
V
Viatte
Nadine
N
Maisonneuve
Claude
C
Pichard
Laurence
L
Genton
D000328:Adult; D000368:Aged; D015992:Body Mass Index; D002153:Calorimetry, Indirect / Q000295:instrumentation* / Q000379:methods*; D002245:Carbon Dioxide / Q000032:analysis*; D004734:Energy Metabolism / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010100:Oxygen / Q000032:analysis*; D011446:Prospective Studies
D002245:Carbon Dioxide; D010100:Oxygen
Energy expenditure; Indirect calorimetry; Validation