[Effect of pulchinenoside in regulating FLS SFRP2 expression of RA model rats].
OBJECTIVE
To study the effect of pulchinenoside (PULC) in modulating SFRP2 expression in fibroblast-like synoviocytes (FLS) of rheumatoid arthritis (RA) model rats.


METHODS
The effect of PULC in treating RA rats was evaluated by rat arthritis score and paw swelling score. The inhibitory effect of PULC on FLS proliferation was detected by MTT reagent. The effects of PULC gavage treatment in modulating gene expression of FLS SFRP2, critical gene beta-catenin of Wnt pathway and downstream effector genes C-myc of of Wnt pathway were detected by RT-PCR and Western blotting.


RESULTS
PULC had a significant effect in treating RA rats and that SFRP2 expression was down-regulated in FLS. After PULC gavage treatment, FLS SFRP2 expression was obviously up-regulated, whereas beta-catenin and C-myc gene expressions were significantly down-regulated.


CONCLUSIONS
PULC can inhibit abnormal proliferation of synovial membrane by modulating Wnt pathway of RA rats.
Anhui Key Laboratory of Poultry Disease Monitoring, Anhui Science and Technology University, Bengbu 233100, China. fensheng1981@126.com
Cheng-Gui
CG
Miao
Jian-Ting
JT
Yang
Hua-Qi
HQ
He
Shi-Tang
ST
Ma
Guo-Liang
GL
Zhou
Min
M
Gao
Jian
J
Liu
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000188:drug therapy* / Q000378:metabolism; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000494:pharmacology*; D013583:Synovial Membrane / Q000187:drug effects* / Q000378:metabolism; D000094742:Secreted Frizzled-Related Proteins
D008565:Membrane Proteins; D012503:Saponins; C503650:Sfrp2 protein, rat; D000094742:Secreted Frizzled-Related Proteins