[Quantitative analysis of protein concentration by absorption peak integration method of UV spectroscopy--taking alkaline phosphatase as an example].
Adsorption of alkaline phosphatase (APase) on minerals (goethite, montmorillonite) and sediments from Taihu Lake was studied. However, the concentration of alkaline phosphatase in the supernatant cannot be analyzed by the adsorption at 280 nm due to that the UV spectroscopy was changed. Quantitative analysis of alkaline phosphatase in the supernatant by the absorption peak (252-305 nm) integration method of UV spectroscopy was developed. This method determined the concentrations of alkaline phosphatase well. Compared to the results of Bradford, the absorption peak integration method can determine the concentrations of supernatant fast, conveniently, and accurately. This method can also be applied to other protein solution analysis and similar experiments. The drawbacks of traditional single wavelength method (280nm) were overcome to some extent based on the method of absorption peak integration.
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. yrzhu1984@163.com
Yuan-Rong
YR
Zhu
Feng-Chang
FC
Wu
Ying
Y
Lin
D000327:Adsorption; D000469:Alkaline Phosphatase / Q000737:chemistry*; D001546:Bentonite / Q000737:chemistry; D058085:Iron Compounds / Q000737:chemistry; D060106:Lakes; D008903:Minerals / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D058085:Iron Compounds; D008903:Minerals; D011506:Proteins; D001546:Bentonite; C094886:goethite; D000469:Alkaline Phosphatase