A novel electrochemical method for efficient reduction of disulfide bonds in peptides and proteins prior to MS detection.
A novel electrochemical (EC) method for fast and efficient reduction of the disulfide bonds in proteins and peptides is presented. The method does not use any chemical agents and is purely instrumental. To demonstrate the performance of the EC reactor cell online with electrospray mass spectrometry, insulin and somatostatin were used as model compounds. Efficient reduction is achieved in continuous infusion mode using an EC reactor cell with a titanium-based working electrode. Under optimized conditions, the presented method shows almost complete reduction of insulin and somatostatin. The method does not require any special sample preparation, and the EC reactor cell makes it suitable for automation. Online EC reduction followed by collision-induced dissociation fragmentation of somatostatin showed more backbone cleavages and improved sequence coverage. By adjusting the settings, the EC reaction efficiency was gradually changed from partial to full disulfide bonds reduction in α-lactalbumin, and the expected shift in charge state distribution has been demonstrated. The reduction can be controlled by adjusting the square-wave pulse, flow rate or mobile phase composition. We have shown the successful use of an EC reactor cell for fast and efficient reduction of disulfide bonds for online mass spectrometry of proteins and peptides. The possibility of online and gradual disulfide bond reduction adds a unique dimension to characterization of disulfide bonds in mid-and top-down proteomics applications.
Agnieszka
A
Kraj
Hendrik-Jan
HJ
Brouwer
Nico
N
Reinhoud
Jean-Pierre
JP
Chervet
D000595:Amino Acid Sequence; D004220:Disulfides / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D007328:Insulin / Q000737:chemistry*; D007768:Lactalbumin / Q000737:chemistry*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010449:Peptide Mapping; D013004:Somatostatin / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D004220:Disulfides; D007328:Insulin; D013004:Somatostatin; D007768:Lactalbumin