Smart-seq2 for sensitive full-length transcriptome profiling in single cells.
Single-cell gene expression analyses hold promise for characterizing cellular heterogeneity, but current methods compromise on either the coverage, the sensitivity or the throughput. Here, we introduce Smart-seq2 with improved reverse transcription, template switching and preamplification to increase both yield and length of cDNA libraries generated from individual cells. Smart-seq2 transcriptome libraries have improved detection, coverage, bias and accuracy compared to Smart-seq libraries and are generated with off-the-shelf reagents at lower cost.
Ludwig Institute for Cancer Research, Stockholm, Sweden.
Simone
S
Picelli
Åsa K
ÅK
Björklund
Omid R
OR
Faridani
Sven
S
Sagasser
Gösta
G
Winberg
Rickard
R
Sandberg
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D057809:HEK293 Cells; D006801:Humans; D016133:Polymerase Chain Reaction; D059010:Single-Cell Analysis; D059467:Transcriptome
D018076:DNA, Complementary