Effect of collagen I gel on apoptosis of rat hepatic stellate cells.
Activated hepatic stellate cells (HSC) are a major source offibrous proteins in cirrhotic liver. Inducing or accelerating their apoptosis is a potential way of liver fibrosis treatment. Extracellular matrix (ECM) surrounding cells in tissue affects their differentiation, migration, proliferation and function. Type I collagen is the main ECM component in fibrotic liver. We have examined how this protein modifies apoptosis of normal rat HSC induced by gliotoxin, cycloheximide and cytochalasin D in vitro and spontaneous apoptosis of HSC isolated from CCl4-damaged liver. We have found that type I collagen gel enhances HSC apoptosis regardless of the agent triggering this process.
Department of Medical Biochemistry, Charles University in Prague, Faculty of Medicine, Hradec Králové, Czech Republic.
Lenka
L
Bittnerová
Alena
A
Jiroutová
Emil
E
Rudolf
Martina
M
Rezácová
Jirí
J
Kanta
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002251:Carbon Tetrachloride; D018929:Cell Culture Techniques; D024042:Collagen Type I / Q000494:pharmacology*; D003513:Cycloheximide; D015638:Cytochalasin D; D004195:Disease Models, Animal; D005912:Gliotoxin; D055166:Hepatic Stellate Cells / Q000187:drug effects* / Q000473:pathology; D008103:Liver Cirrhosis / Q000473:pathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D024042:Collagen Type I; D015638:Cytochalasin D; D005912:Gliotoxin; D003513:Cycloheximide; D002251:Carbon Tetrachloride