Enzymatic hydrolysis preparation of mono-O-lauroylsucrose via a mono-O-lauroylraffinose intermediate.
1'-O-Lauroylsucrose and 6'-O-lauroylsucrose were formed through hydrolysis of the C-6″ galactose group of 1'-O-lauroylraffinose and 6'-O-lauroylraffinose, respectively, in the presence of α-galactosidase. The enzymatic hydrolysis of 1'-O-lauroylraffinose and 6'-O-lauroylraffinose is discussed in detail. Acetic acid-sodium acetate was chosen as the buffer solution of the enzymatic hydrolysis reaction. The optimum conditions for the enzymatic hydrolysis reaction were as follows: buffer solution, pH 3.8; enzymatic time, 48 h; and enzymatic temperature, 37 °C. Under the optimal process conditions, the efficiency of α-galactosidase was ca. 82.6%. The isomers were fully compared in solubility, hydrophile-lipophile balance (HLB) values, critical micelle concentration (CMC), and thermal stability. The results showed that all lauroylsucrose isomers have similar solubilities in polar solvent, HLB values, CMC values, and thermal stabilities.
Department of Food Science and Engineering, College of Science and Engineering, Jinan University , Number 601 Huangpu Road West, Guangzhou 510632, People's Republic of China.
Yuyun
Y
Lu
Rian
R
Yan
Xiang
X
Ma
Yong
Y
Wang
Yuankui
Y
Sun
Zhongming
Z
Luo
D000215:Acylation; D055162:Biocatalysis; D003358:Cosmetics / Q000737:chemistry / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000378:metabolism; D005079:Excipients / Q000737:chemistry / Q000378:metabolism; D005503:Food Additives / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D007202:Indicators and Reagents / Q000737:chemistry / Q000378:metabolism; D007536:Isomerism; D007848:Laurates / Q000737:chemistry / Q000378:metabolism*; D007850:Lauric Acids / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D011887:Raffinose / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D013395:Sucrose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D013501:Surface-Active Agents / Q000737:chemistry / Q000378:metabolism*; D000519:alpha-Galactosidase / Q000378:metabolism*
D003358:Cosmetics; D004800:Enzymes, Immobilized; D005079:Excipients; D005503:Food Additives; D007202:Indicators and Reagents; D007848:Laurates; D007850:Lauric Acids; D013501:Surface-Active Agents; C030358:lauric acid; D013395:Sucrose; D000519:alpha-Galactosidase; D011887:Raffinose