A simplified and cost-effective method combining real-time PCR and pyrosequencing for detection of aac(6')-Ib-cr gene.
We previously described a pyrosequencing-based method able to guarantee detection of aac(6')-Ib-cr by characterizing precisely the single nucleotide polymorphisms leading to Trp102Arg and Asp179Tyr. By gaining in simplicity and cost-effectiveness, through the use of real-time PCR, we propose here a cutting-edge evolution of our existing pyrosequencing method.
CHU Reims, Hôpital Robert Debré, Laboratoire de Bactériologie-Virologie-Hygiène, F-51092 Reims, France; UFR Médecine, EA 4687 ERA, SFR CAP-Santé, Université de Reims Champagne-Ardenne, F-51092 Reims, France. Electronic address: tguillard@chu-reims.fr.
Thomas
T
Guillard
Nicolas
N
Fontaine
Anne
A
Limelette
Anne-Laure
AL
Lebreil
Janick
J
Madoux
Christophe
C
de Champs
D000123:Acetyltransferases / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification*; D003362:Cost-Benefit Analysis; D004269:DNA, Bacterial / Q000302:isolation & purification*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000302:isolation & purification; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D000123:Acetyltransferases; C019681:aminoglycoside acetyltransferase
Plasmid-mediated quinolone resistance; Pyrosequencing; Real-time PCR; SYBR Green; aac(6′)-Ib-cr