Hydrogel microspheres for stabilization of an antioxidant enzyme: effect of emulsion cross-linking of a dual polysaccharide system on the protection of enzyme activity.
Catalase is an antioxidant enzyme abundant in natural resources. However, the enzyme is usually inactivated by gastric acid and digestive enzymes after oral ingestion. In this study, carboxymethyl chitosan (CM-chitosan) and hyaluronic acid (HA) conjugate hydrogel microspheres have been prepared by an emulsion cross-linking technique to retain the activity of catalase in simulated gastrointestinal (GI) fluids. Cross-linking reduced the swelling capability and increased the resistance toward hyaluronidase digestion of prepared HA-CM-chitosan hydrogel microspheres. Catalase entrapped in the hydrogel microspheres exhibited superior stability over a wide pH range (pH 2.0 and 6.0-8.0) as compared to the native enzyme. The entrapped catalase was also protected against degradation by digestive enzymes. Following the treatments, the catalase-loaded microspheres, in contrast to native catalase, could effectively decrease the intracellular H2O2 level and protect HT-29 colonic epithelial cells against H2O2-induced oxidative damage to preserve cell viability. These results suggested that the HA-CM-chitosan hydrogel microspheres can be used for entrapment, protection and intestinal delivery of catalase for H2O2 scavenging.
Department of Biotechnology, Vanung University, Chung-Li 320, Taiwan.
Deh-Wei
DW
Tang
Department of Polymer Materials, Vanung University, Chung-Li 320, Taiwan.
Shu-Huei
SH
Yu
Department of Biochemistry, School of Medicine, Taipei Medical University, Taipei 110, Taiwan; Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan.
Wen-Shin
WS
Wu
Department of Polymer Materials, Vanung University, Chung-Li 320, Taiwan.
Hao-Ying
HY
Hsieh
Department of Biotechnology, Vanung University, Chung-Li 320, Taiwan.
Yi-Chin
YC
Tsai
Department of Biochemistry, School of Medicine, Taipei Medical University, Taipei 110, Taiwan; Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan. Electronic address: flmi530326@tmu.edu.tw.
Fwu-Long
FL
Mi
D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism*; D002374:Catalase / Q000737:chemistry / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D004655:Emulsions / Q000737:chemistry*; D019073:HT29 Cells; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008863:Microspheres; D011134:Polysaccharides / Q000737:chemistry*
D000975:Antioxidants; D004655:Emulsions; D011134:Polysaccharides; C514968:carboxymethyl-chitosan; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006820:Hyaluronic Acid; D048271:Chitosan; D006861:Hydrogen Peroxide; D002374:Catalase
Antioxidant enzyme; Catalase; Chitosan; Microspheres; Stabilization