Status of antioxidant enzyme: glutathione peroxidase and total polyphenol level in plasma of Tunisian patients suffering from colorectal and gastric cancer: interaction with clinical outcome.
In our case-control study, we measure the antioxidant status by dosing enzymes involved in oxidant stress in plasma of patients with colorectal and gastric cancer, and in the second step, we investigate the impact of chemotherapy before and after surgery on plasma antioxidant status and polyphenols in patients. Blood serum was collected from patients with stomach and colorectal cancer before conventional treatment, and glutathione peroxidase (GSHPX) enzyme activities and total polyphenols were determined by spectrophotometric methods. In our study, we found a significant decrease in glutathione peroxidase activity in plasma of patients compared with controls (P = 0.02), although we did not find a significant association between total polyphenols and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) or ABTS in plasma of colorectal and stomach cancer compared with control; furthermore, we observed no significant difference in the average plasma polyphenols in patients treated with chemotherapy before and after surgery. We have shown the decrease in GSHPX activity in plasma of cases with colorectal and gastric cancer, and this decrease reflects that the oxidative stress is associated with tumor tract and related to oxidative metabolism; however, no association was found between total polyphenols and ABTS in our study.
Laboratory of Genetics, Immunology and Human Pathology, Faculty of Science, El MANAR, Campus University, 2092, Tunis, Tunisia, olfa.baroudi@yahoo.fr.
Olfa
O
Baroudi
Sonia Ben
SB
Younes
Amel
A
Mézlini
Yves Jean
YJ
Bignon
Imen
I
Medimeg
Nancy
N
Uhrhammer
Amel Ben Ammar E L
AB
Gaiied
Soufia Chabchoub
SC
Ellouz
D000975:Antioxidants / Q000378:metabolism*; D052160:Benzothiazoles / Q000097:blood / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000097:blood* / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology; D059808:Polyphenols / Q000097:blood* / Q000378:metabolism; D013274:Stomach Neoplasms / Q000097:blood* / Q000201:enzymology / Q000378:metabolism*; D013451:Sulfonic Acids / Q000097:blood / Q000378:metabolism
D000975:Antioxidants; D052160:Benzothiazoles; D059808:Polyphenols; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D005979:Glutathione Peroxidase