Insights on the genotype distribution among Cryptococcus neoformans and C. gattii Portuguese clinical isolates.
This study provides a comprehensive picture of the C. neoformans/C. gattii molecular types most often associated with human cryptococcosis in Portugal and assesses the impact of C. gattii in these infections. One hundred and twenty-two clinical isolates, from distinct patients, were identified as C. neoformans and genotyped by URA5-RFLP, with the molecular types VNI (45.5 %) and VNIII (30.9 %) being the most commonly found ones. The molecular types VNII (11.4 %) and VNIV (11.4 %) were less abundant. One patient was found to be infected with a VGII isolate. This patient exhibited unusual clinical symptoms of cryptococcosis, reinforcing the suspicion for the presence of a different genotypic pattern, as determined afterwards. This case was detected in 2007 and is the first report of a potential autochthonous C. gattii infection case in Portugal, as the patient revealed no historical record of travelling outside the country.
A P
AP
Maduro
K
K
Mansinho
F
F
Teles
I
I
Silva
W
W
Meyer
M L
ML
Martins
J
J
Inácio
D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology; D000328:Adult; D000368:Aged; D003453:Cryptococcosis / Q000382:microbiology*; D003454:Cryptococcus / Q000235:genetics* / Q000302:isolation & purification; D003455:Cryptococcus neoformans / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D058889:Molecular Typing; D055815:Young Adult