A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude.
The low detection sensitivity of enzyme linked immunosorbent assays (ELISAs) is a central problem in science and limits progress in multiple areas of biology and medicine. In this report we demonstrate that the hydrocyanines, a family of fluorescent reactive oxygen species (ROS) probes, can act as turn on fluorescent horseradish peroxidase (HRP) probes and thereby increase the sensitivity of conventional ELISAs by two orders of magnitude.
Department of Bioengineering, University of California, Berkeley, California 94720, USA. nmurthy@berkeley.edu.
Abhinav P
AP
Acharya
Parsa M
PM
Nafisi
Austin
A
Gardner
Joanna L
JL
Mackay
Kousik
K
Kundu
Sanjay
S
Kumar
Niren
N
Murthy
D002232:Carbocyanines / Q000737:chemistry; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000032:analysis*; D057230:Limit of Detection
D002232:Carbocyanines; D005456:Fluorescent Dyes; D006735:Horseradish Peroxidase