Role of Art v 3 in pollinosis of patients allergic to Pru p 3.
BACKGROUND
Food allergy caused by lipid transfer protein (LTP) from peach (Pru p 3) is frequently associated with sensitization to mugwort LTP (Art v 3). Although in vitro cross-reactivity is already well known, it has yet to be elucidated whether a pollen LTP can induce rhinitis.


OBJECTIVE
The aim of this study was to investigate whether mugwort LTP could elicit respiratory symptoms and whether a primary food LTP allergy could lead to a respiratory allergy.


METHODS
Patients with confirmed Pru p 3 allergy and control subjects were selected. Immediate responses to nasal allergen provocation tests (NAPTs) with Art v 3, Pru p 3, and mugwort were assessed by using the visual analog scale score, total nasal symptom score, and acoustic rhinometry. Tryptase and cysteinyl leukotriene (cysLT) levels were measured in nasal lavage fluid. Immunoblotting, ELISAs, and ELISA inhibition assays were also performed.


RESULTS
Fifteen patients and 9 control subjects were selected. NAPT results with Art v 3 and Pru p 3 showed significant changes in acoustic rhinometry, visual analog scale scores, total nasal symptom scores, and cysLT levels (P < .001). Tryptase levels were only increased in NAPTs with Pru p 3. NAPTs with mugwort were used in those patients who were only sensitized to Art v 3, with similar results (P < .05). No significant changes were detected in control subjects.


CONCLUSIONS
The results demonstrated that a pollen LTP can elicit rhinitis in sensitized patients. Findings also suggest that a primary sensitization to Pru p 3 can lead to a respiratory allergy through cross-reactivity.
Unitat d'Al·lèrgia, Institut Clínic del Tòrax (ICT), Hospital Clinic, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona, Barcelona, Spain. Electronic address: jasanche@clinic.ub.es.
Jaime
J
Sánchez-López
Centro de Biotecnología y Genómica de Plantas (CBGP), Universidad Politécnica de Madrid, Campus Montegancedo, Madrid, Spain.
Leticia
L
Tordesillas
Servei d'Immunologia, Centre de Diagnòstic Biomèdic (CDB), Hospital Clinic, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
Mariona
M
Pascal
Unitat d'Al·lèrgia, Institut Clínic del Tòrax (ICT), Hospital Clinic, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona, Barcelona, Spain.
Rosa
R
Muñoz-Cano
Centro de Biotecnología y Genómica de Plantas (CBGP), Universidad Politécnica de Madrid, Campus Montegancedo, Madrid, Spain.
María
M
Garrido
Unitat d'Al·lèrgia, Institut Clínic del Tòrax (ICT), Hospital Clinic, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona, Barcelona, Spain.
Maria
M
Rueda
Servei d'Immunologia, Centre de Diagnòstic Biomèdic (CDB), Hospital Clinic, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
Ramón
R
Vilella
Unitat d'Al·lèrgia, Institut Clínic del Tòrax (ICT), Hospital Clinic, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona, Barcelona, Spain.
Antonio
A
Valero
Centro de Biotecnología y Genómica de Plantas (CBGP), Universidad Politécnica de Madrid, Campus Montegancedo, Madrid, Spain.
Araceli
A
Díaz-Perales
Unitat d'Al·lèrgia, Institut Clínic del Tòrax (ICT), Hospital Clinic, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona, Barcelona, Spain.
César
C
Picado
Unitat d'Al·lèrgia, Institut Clínic del Tòrax (ICT), Hospital Clinic, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona, Barcelona, Spain.
Joan
J
Bartra
D000328:Adult; D052179:Antigens, Plant / Q000276:immunology*; D016022:Case-Control Studies; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005512:Food Hypersensitivity / Q000150:complications* / Q000175:diagnosis / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010940:Plant Proteins / Q000276:immunology*; D006255:Rhinitis, Allergic, Seasonal / Q000150:complications* / Q000175:diagnosis / Q000276:immunology*; D012882:Skin Tests; D055815:Young Adult
D052179:Antigens, Plant; C495881:Art v 3 allergen, Artemisia vulgaris; D010940:Plant Proteins; C463181:Pru p 3 allergen; D007073:Immunoglobulin E
Art v 3; Lipid transfer protein; Pru p 3; food allergy; nasal allergen provocation test; pollinosis