Preventive and curative effects of Apple latent spherical virus vectors harboring part of the target virus genome against potyvirus and cucumovirus infections.
Apple latent spherical virus (ALSV)-based vectors experimentally infect a broad range of plant species without causing symptoms and can effectively induce stable virus-induced gene silencing in plants. Here, we show that pre-infection of ALSV vectors harboring part of a target viral genome (we called ALSV vector vaccines here) inhibits the multiplication and spread of the corresponding challenge viruses [Bean yellow mosaic virus, Zucchini yellow mosaic virus (ZYMV), and Cucumber mosaic virus (CMV)] by a homology-dependent resistance. Further, the plants pre-infected with an ALSV vector having genome sequences of both ZYMV and CMV were protected against double inoculation of ZYMV and CMV. More interestingly, a curative effect of an ALSV vector vaccine could also be expected in ZYMV-infected cucumber plants, because the symptoms subsided on subsequent inoculation with an ALSV vector vaccine. This may be due to the invasion of ALSV, but not ZYMV, in the shoot apical meristem of cucumber.
Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan.
Akihiro
A
Tamura
Takahiro
T
Kato
Ayano
A
Taki
Mikako
M
Sone
Nozomi
N
Satoh
Noriko
N
Yamagishi
Tsubasa
T
Takahashi
Bo-Song
BS
Ryo
Tomohide
T
Natsuaki
Nobuyuki
N
Yoshikawa
D018553:Cucumis sativus / Q000821:virology; D017799:Cucumovirus / Q000254:growth & development* / Q000472:pathogenicity; D020868:Gene Silencing; D016678:Genome; D010935:Plant Diseases / Q000517:prevention & control* / Q000821:virology*; D017800:Potyvirus / Q000254:growth & development* / Q000472:pathogenicity; D012328:RNA Viruses / Q000235:genetics*; D011995:Recombination, Genetic; D014762:Viral Interference
ALSV vector; BYMV; CMV; Cross protection; Preventive and curative effects; VIGS; ZYMV