Characterization of microRNA expression profiles in 3T3-L1 adipocytes overexpressing C10orf116.
Our data in the previous report demonstrated that C10orf116 (AFRO) is an adipocyte lineage-specific nuclear factor that can modulate the master adipogenesis transcription factors early during differentiation. However, more precise functional properties of this gene need to be clarified and await further investigation. Therefore, in this study, we performed an expression profile of cellular MicroRNAs (miRNAs) in the C10orf116 overexpression 3T3-L1 adipocytes and performed target prediction and functional enrichment of the differentially expressed miRNAs. Our study identified 34 miRNAs up-regulated in the 3T3-L1 adipocytes stably overexpressing C10orf116, whereas 43 miRNAs up-regulated in the control cells. The target genes of differentially expressed miRNAs were found to be involved in multiple signalling pathways, such as Wnt, TGF-beta, MAPK, Jak-STAT, insulin signalling pathway, et al. Our data provided novel information for the identification of C10orf116.
Department of Newborn Infants, Nanjing Children's Hospital of Nanjing Medical University, Nanjing, 210008, China, jieqiu.nj@hotmail.com.
Jie
J
Qiu
Xiao-guang
XG
Zhou
Xiao-yu
XY
Zhou
Chun
C
Zhu
Chun-mei
CM
Shi
Chen-bo
CB
Ji
Rui
R
Cheng
Yong
Y
Li
Xi-rong
XR
Guo
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D015870:Gene Expression; D005786:Gene Expression Regulation; D050356:Lipid Metabolism / Q000235:genetics; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D015203:Reproducibility of Results; D059467:Transcriptome; D014162:Transfection
C584291:ADIRF protein, human; D035683:MicroRNAs; D009687:Nuclear Proteins