Engineering a synthetic pathway in cyanobacteria for isopropanol production directly from carbon dioxide and light.
Production of alternate fuels or chemicals directly from solar energy and carbon dioxide using engineered cyanobacteria is an attractive method to reduce petroleum dependency and minimize carbon emissions. Here, we constructed a synthetic pathway composed of acetyl-CoA acetyl transferase (encoded by thl), acetoacetyl-CoA transferase (encoded by atoAD), acetoacetate decarboxylase (encoded by adc) and secondary alcohol dehydrogenase (encoded by adh) in Synechococcus elongatus strain PCC 7942 to produce isopropanol. The enzyme-coding genes, heterogeneously originating from Clostridium acetobutylicum ATCC 824 (thl and adc), Escherichia coli K-12 MG1655 (atoAD) and Clostridium beijerinckii (adh), were integrated into the S. elongatus genome. Under the optimized production conditions, the engineered cyanobacteria produced 26.5 mg/L of isopropanol after 9 days.
Laboratory for Bioinformatics, Graduate School of Systems Life Sciences, Kyushu University, 804 Westwing, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Tamami
T
Kusakabe
Tsuneyuki
T
Tatsuke
Keigo
K
Tsuruno
Yasutaka
Y
Hirokawa
Shota
S
Atsumi
James C
JC
Liao
Taizo
T
Hanai
D019840:2-Propanol / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism*; D046969:Clostridium acetobutylicum / Q000201:enzymology / Q000235:genetics; D046970:Clostridium beijerinckii / Q000201:enzymology / Q000235:genetics; D048168:Escherichia coli K12 / Q000201:enzymology / Q000235:genetics; D008027:Light; D060847:Metabolic Engineering; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D046940:Synechococcus / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D011994:Recombinant Proteins; D002245:Carbon Dioxide; D019840:2-Propanol
Cyanobacteria; Isopropanol production; Synthetic pathway