Metabolic profiles and free radical scavenging activity of Cordyceps bassiana fruiting bodies according to developmental stage.
The metabolic profiles of Cordyceps bassiana according to fruiting body developmental stage were investigated using gas chromatography-mass spectrometry. We were able to detect 62 metabolites, including 48 metabolites from 70% methanol extracts and 14 metabolites from 100% n-hexane extracts. These metabolites were classified as alcohols, amino acids, organic acids, phosphoric acids, purine nucleosides and bases, sugars, saturated fatty acids, unsaturated fatty acids, or fatty amides. Significant changes in metabolite levels were found according to developmental stage. Relative levels of amino acids, purine nucleosides, and sugars were higher in development stage 3 than in the other stages. Among the amino acids, valine, isoleucine, lysine, histidine, glutamine, and aspartic acid, which are associated with ABC transporters and aminoacyl-tRNA biosynthesis, also showed higher levels in stage 3 samples. The free radical scavenging activities, which were significantly higher in stage 3 than in the other stages, showed a positive correlation with purine nucleoside metabolites such as adenosine, guanosine, and inosine. These results not only show metabolic profiles, but also suggest the metabolic pathways associated with fruiting body development stages in cultivated C. bassiana.
College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea.
Sun-Hee
SH
Hyun
Seok-Young
SY
Lee
Gi-Ho
GH
Sung
Seong Hwan
SH
Kim
Hyung-Kyoon
HK
Choi
D000596:Amino Acids / Q000302:isolation & purification; D002241:Carbohydrates / Q000302:isolation & purification; D032661:Cordyceps / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D000431:Ethanol; D005227:Fatty Acids / Q000302:isolation & purification; D016166:Free Radical Scavengers / Q000302:isolation & purification; D048690:Fruiting Bodies, Fungal / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006586:Hexanes; D053858:Metabolic Networks and Pathways / Q000502:physiology*; D055442:Metabolome; D009705:Nucleosides / Q000302:isolation & purification; D052616:Solid Phase Extraction; D012997:Solvents
D000596:Amino Acids; D002241:Carbohydrates; D005227:Fatty Acids; D016166:Free Radical Scavengers; D006586:Hexanes; D009705:Nucleosides; D012997:Solvents; C026385:n-hexane; D000431:Ethanol