Epigenetic regulatory elements associate with specific histone modifications to prevent silencing of telomeric genes.
In eukaryotic cells, transgene expression levels may be limited by an unfavourable chromatin structure at the integration site. Epigenetic regulators are DNA sequences which may protect transgenes from such position effect. We evaluated different epigenetic regulators for their ability to protect transgene expression at telomeres, which are commonly associated to low or inconsistent expression because of their repressive chromatin environment. Although to variable extents, matrix attachment regions (MARs), ubiquitous chromatin opening element (UCOE) and the chicken cHS4 insulator acted as barrier elements, protecting a telomeric-distal transgene from silencing. MARs also increased the probability of silent gene reactivation in time-course experiments. Additionally, all MARs improved the level of expression in non-silenced cells, unlike other elements. MARs were associated to histone marks usually linked to actively expressed genes, especially acetylation of histone H3 and H4, suggesting that they may prevent the spread of silencing chromatin by imposing acetylation marks on nearby nucleosomes. Alternatively, an UCOE was found to act by preventing deposition of repressive chromatin marks. We conclude that epigenetic DNA elements used to enhance and stabilize transgene expression all have specific epigenetic signature that might be at the basis of their mode of action.
Laboratory of Molecular Biotechnology, Center for Biotechnology UNIL-EPFL, University of Lausanne, 1015 Lausanne, Switzerland.
Stefano
S
Majocchi
Elena
E
Aritonovska
Nicolas
N
Mermod
D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000737:chemistry*; D004843:Epistasis, Genetic; D020868:Gene Silencing; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism*; D006801:Humans; D045170:Matrix Attachment Regions; D012045:Regulatory Sequences, Nucleic Acid; D016615:Telomere; D019076:Transgenes
D002843:Chromatin; D006657:Histones; D004247:DNA