The plastic nature of the human bone-periodontal ligament-tooth fibrous joint.
This study investigates bony protrusions within a narrowed periodontal ligament space (PDL-space) of a human bone-PDL-tooth fibrous joint by mapping structural, biochemical, and mechanical heterogeneity. Higher resolution structural characterization was achieved via complementary atomic force microscopy (AFM), nano-transmission X-ray microscopy (nano-TXM), and microtomography (MicroXCT™). Structural heterogeneity was correlated to biochemical and elemental composition, illustrated via histochemistry and microprobe X-ray fluorescence analysis (μ-XRF), and mechanical heterogeneity evaluated by AFM-based nanoindentation. Results demonstrated that the narrowed PDL-space was due to invasion of bundle bone (BB) into PDL-space. Protruded BB had a wider range with higher elastic modulus values (2-8GPa) compared to lamellar bone (0.8-6GPa), and increased quantities of Ca, P and Zn as revealed by μ-XRF. Interestingly, the hygroscopic 10-30μm interface between protruded BB and lamellar bone exhibited higher X-ray attenuation similar to cement lines and lamellae within bone. Localization of the small leucine rich proteoglycan biglycan (BGN) responsible for mineralization was observed at the PDL-bone interface and around the osteocyte lacunae. Based on these results, it can be argued that the LB-BB interface was the original site of PDL attachment, and that the genesis of protruded BB identified as protrusions occurred as a result of shift in strain. We emphasize the importance of bony protrusions within the context of organ function and that additional study is warranted.
Division of Biomaterials and Bioengineering, Department of Preventive and Restorative Dental Sciences, University of California San Francisco, San Francisco, CA, USA. Electronic address: sunita.ho@ucsf.edu.
Sunita P
SP
Ho
Michael P
MP
Kurylo
Kathryn
K
Grandfield
Jonathan
J
Hurng
Ralf-Peter
RP
Herber
Mark I
MI
Ryder
Virginia
V
Altoe
Shaul
S
Aloni
Jian Q
JQ
Feng
Samuel
S
Webb
Grayson W
GW
Marshall
Donald
D
Curtis
Joy C
JC
Andrews
Piero
P
Pianetta
D058578:Biglycan / Q000378:metabolism; D001696:Biomechanical Phenomena; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000502:physiology* / Q000648:ultrastructure; D002118:Calcium / Q000378:metabolism; D003739:Dental Cementum / Q000000981:diagnostic imaging / Q000502:physiology; D055119:Elastic Modulus; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D000071235:Fibromodulin; D005453:Fluorescence; D006801:Humans; D007150:Immunohistochemistry; D007596:Joints / Q000502:physiology* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D008954:Models, Biological; D010513:Periodontal Ligament / Q000166:cytology / Q000000981:diagnostic imaging / Q000502:physiology* / Q000648:ultrastructure; D010758:Phosphorus / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D013499:Surface Properties; D014070:Tooth / Q000000981:diagnostic imaging / Q000502:physiology* / Q000648:ultrastructure; D055114:X-Ray Microtomography; D015032:Zinc / Q000378:metabolism
D058578:Biglycan; D016326:Extracellular Matrix Proteins; C000604330:FMOD protein, human; D011509:Proteoglycans; D000071235:Fibromodulin; D010758:Phosphorus; D015032:Zinc; D002118:Calcium
Alveolar bone; Bone functional adaptation; Bone–PDL–tooth fibrous joint; Bundle bone; Discontinuities; Periodontal ligament