Receptor specificity does not affect replication or virulence of the 2009 pandemic H1N1 influenza virus in mice and ferrets.
Human influenza viruses predominantly bind α2,6 linked sialic acid (SA) while avian viruses bind α2,3 SA-containing complex glycans. Virulence and tissue tropism of influenza viruses have been ascribed to this binding preference. We generated 2009 pandemic H1N1 (pH1N1) viruses with either predominant α2,3 or α2,6 SA binding and evaluated these viruses in mice and ferrets. The α2,3 pH1N1 virus had similar virulence in mice and replicated to similar titers in the respiratory tract of mice and ferrets as the α2,6 and WT pH1N1 viruses. Immunohistochemical analysis determined that all viruses infected similar cell types in ferret lungs. There is increasing evidence that receptor specificity of influenza viruses is more complex than the binary model of α2,6 and α2,3 SA binding and our data suggest that influenza viruses use a wide range of SA moieties to infect host cells.
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Seema S
SS
Lakdawala
Angela R
AR
Shih
Akila
A
Jayaraman
Elaine W
EW
Lamirande
Ian
I
Moore
Myeisha
M
Paskel
Heather
H
Kenney
Ram
R
Sasisekharan
Kanta
K
Subbarao
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005289:Ferrets; D054884:Host-Pathogen Interactions; D007150:Immunohistochemistry; D053118:Influenza A Virus, H1N1 Subtype / Q000472:pathogenicity* / Q000502:physiology*; D008168:Lung / Q000473:pathology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000473:pathology / Q000821:virology; D011991:Receptors, Virus / Q000378:metabolism*; D012794:Sialic Acids / Q000378:metabolism*; D019562:Viral Load; D056189:Viral Tropism; D014774:Virulence; D014779:Virus Replication
D011991:Receptors, Virus; D012794:Sialic Acids
Influenza virus; Receptor specificity; Replication; Tissue tropism; Virulence