E6 and E7 of human papillomavirus type 18 and UVB irradiation corporately regulate interleukin-6 and interleukin-8 expressions in basal cell carcinoma.
The lack of a human papillomavirus (HPV)-infected skin cancer cell line has hampered the investigation of the interaction of UV and HPV in skin carcinogenesis. We identified a human basal cell carcinoma (BCC-1/KMC) cell line integrated with E6 and E7 genes of high-risk HPV type 18 and demonstrated that repression of E6 and E7 results in proliferation inhibition. Sublethal ultraviolet-B (UVB) irradiation induced the expressions of interleukin-6 (IL-6) and interleukin-8 (IL-8), as well as viral E6 and E7 genes, in BCC-1/KMC cells. When E6 and E7 expressions were inhibited, IL-6/IL-8 expressions were repressed. Furthermore, IL-6/IL-8 remained inducible by UVB irradiation when E6 and E7 were inhibited. These results indicated that IL-6 and IL-8 can be upregulated by viral E6 and E7 proteins without UVB irradiation. Moreover, chronic exposure to UVB upregulates IL-6 and IL-8 when E6/E7 is induced by UVB.
Department of Dermatology, Chung Shan Medical University Hospital, Taichung City, Taiwan; Institute of Medicine, School of Medicine, Chung Shan Medical University, Taichung City, Taiwan.
Yu-Ping
YP
Hsiao
Jen-Hung
JH
Yang
Wen-Jun
WJ
Wu
Meng-Hsuan
MH
Lin
Gwo-Tarng
GT
Sheu
D002280:Basal Cell Carcinoma / Q000378:metabolism* / Q000821:virology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D015967:Gene Expression Regulation, Viral; D006801:Humans; D007249:Inflammation; D015850:Interleukin-6 / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism*; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D050725:Papillomavirus E7 Proteins / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D012878:Skin Neoplasms / Q000378:metabolism / Q000821:virology; D014466:Ultraviolet Rays; D015854:Up-Regulation
C508606:CXCL8 protein, human; D004268:DNA-Binding Proteins; C052633:E7 protein, Human papillomavirus type 18; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D034741:RNA, Small Interfering; C059731:oncogene protein E7, Human papillomavirus type 16
BCC; HPV; IL-6; IL-8; VUB