Cycles in spatial and temporal chromosomal organization driven by the circadian clock.
Dynamic transitions in the epigenome have been associated with regulated patterns of nuclear organization. The accumulating evidence that chromatin remodeling is implicated in circadian function prompted us to explore whether the clock may control nuclear architecture. We applied the chromosome conformation capture on chip technology in mouse embryonic fibroblasts (MEFs) to demonstrate the presence of circadian long-range interactions using the clock-controlled Dbp gene as bait. The circadian genomic interactions with Dbp were highly specific and were absent in MEFs whose clock was disrupted by ablation of the Bmal1 gene (also called Arntl). We establish that the Dbp circadian interactome contains a wide variety of genes and clock-related DNA elements. These findings reveal a previously unappreciated circadian and clock-dependent shaping of the nuclear landscape.
Department of Biological Chemistry, Center for Epigenetics and Metabolism, University of California Irvine, Irvine, California, USA.
Lorena
L
Aguilar-Arnal
Ofir
O
Hakim
Vishal R
VR
Patel
Pierre
P
Baldi
Gordon L
GL
Hager
Paolo
P
Sassone-Corsi
D056930:ARNTL Transcription Factors / Q000502:physiology; D000818:Animals; D002478:Cells, Cultured; D002875:Chromosomes; D057906:Circadian Clocks; D051379:Mice
D056930:ARNTL Transcription Factors; C538961:Bmal1 protein, mouse