Validation of copy number variants associated with prostate cancer risk and prognosis.
OBJECTIVE
Two recent studies have reported novel heritable copy number variants on chromosomes 2p, 15q, and 12q to be associated with prostate cancer (PCa) risk in non-Hispanic Caucasians. The goal of this study was to determine whether these findings could be independently confirmed in the Caucasian population from the South Texas area.


METHODS
The study subjects consisted of participants of the San Antonio Biomarkers of Risk for PCa cohort and additional cases ascertained in the same metropolitan area. We genotyped all 7 of the reported copy number variants using real-time quantitative polymerase chain reaction in 1,536 (317 cases and 1,219 controls) non-Hispanic Caucasian men, and additionally, we genotyped 632 (191 cases and 441 controls) Hispanic Caucasian men for one of these variants, a deletion on 2p24.3.


RESULTS
Association of the deletion on 2p24.3 with overall PCa risk did not meet our significance criteria but was consistent with previous reports (odds ratio, 1.40; 95% confidence interval 0.99-2.00; P = 0.06). Among Hispanic Caucasians, this deletion is much less prevalent (minor allele frequencies of 0.059 and 0.024 in non-Hispanic and Hispanic Caucasians, respectively) and did not show evidence of association with risk for PCa. Interestingly, among non-Hispanic Caucasians, carrying a homozygous deletion of 2p24.3 was significantly associated with high-grade PCa as defined by Gleason score sum ≥8 (odds ratio, 27.99; 95% confidence interval 1.99-392.6; P = 0.007 [the Fisher exact test]). The remaining 6 copy number variable regions either were not polymorphic in our cohort of non-Hispanic Caucasians or showed no evidence of association.


CONCLUSIONS
Our findings are consistent with the reported observation that a heritable deletion on 2p24.3 is associated with PCa risk in non-Hispanic Caucasians. Additionally, our observations indicate that the 2p24.3 variant is associated with risk for high-grade PCa in a recessive manner. We were unable to replicate any association with PCa for the variants on chromosomes 15q and 12q, which may be explained by regional population differences in low frequency variants and disease heterogeneity.
University of Texas Health Science Center at San Antonio, Department of Cellular and Structural Biology.
August
A
Blackburn
University of Texas Health Science Center at San Antonio, Department of Cellular and Structural Biology.
Desiree
D
Wilson
University of Texas Health Science Center at San Antonio, Department of Epidemiology and Biostatistics.|University of Texas Health Science Center at San Antonio, Cancer Therapy and Research Center.
Jonathan
J
Gelfond
University of Texas Health Science Center at San Antonio, Department of Medicine - Division of Clinical Epidemiology.
Li
L
Yao
University of Texas Health Science Center at San Antonio, Department of Urology.|University of Texas Health Science Center at San Antonio, Cancer Therapy and Research Center.
Javier
J
Hernandez
University of Texas Health Science Center at San Antonio, Department of Urology.|University of Texas Health Science Center at San Antonio, Cancer Therapy and Research Center.
Ian M
IM
Thompson
University of Texas Health Science Center at San Antonio, Department of Cellular and Structural Biology.|University of Texas Health Science Center at San Antonio, Department of Urology.|University of Texas Health Science Center at San Antonio, Cancer Therapy and Research Center.
Robin J
RJ
Leach
University of Texas Health Science Center at San Antonio, Department of Medicine - Division of Clinical Epidemiology.|University of Texas Health Science Center at San Antonio, Cancer Therapy and Research Center.
Donna M
DM
Lehman
D000368:Aged; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics; D015331:Cohort Studies; D056915:DNA Copy Number Variations / Q000235:genetics*; D056726:Genetic Association Studies / Q000379:methods*; D005838:Genotype; D006630:Hispanic or Latino / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D011110:Polymorphism, Genetic; D011379:Prognosis; D011471:Prostatic Neoplasms / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D012307:Risk Factors; D013781:Texas; D044465:White People / Q000235:genetics
Cancer; Deletion; Prognosis; Prostate; Risk