Bioactive nanofibrous scaffolds for regenerative endodontics.
Here we report the synthesis, materials characterization, antimicrobial capacity, and cytocompatibility of novel antibiotic-containing scaffolds. Metronidazole (MET) or Ciprofloxacin/(CIP) was mixed with a polydioxanone (PDS)polymer solution at 5 and 25 wt% and processed into fibers. PDS fibers served as a control. Scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), tensile testing, and high-performance liquid chromatography (HPLC) were used to assess fiber morphology, chemical structure, mechanical properties, and drug release, respectively. Antimicrobial properties were evaluated against those of Porphyromonas gingivalis/Pg and Enterococcus faecalis/Ef. Cytotoxicity was assessed in human dental pulp stem cells (hDPSCs). Statistics were performed, and significance was set at the 5% level. SEM imaging revealed a submicron fiber diameter. FTIR confirmed antibiotic incorporation. The tensile values of hydrated 25 wt% CIP scaffold were significantly lower than those of all other groups. Analysis of HPLC data confirmed gradual, sustained drug release from the scaffolds over 48 hrs. CIP-containing scaffolds significantly (p < .00001) inhibited biofilm growth of both bacteria. Conversely, MET-containing scaffolds inhibited only Pg growth. Agar diffusion confirmed the antimicrobial properties against specific bacteria for the antibiotic-containing scaffolds. Only the 25 wt% CIP-containing scaffolds were cytotoxic. Collectively, this study suggests that polymer-based antibiotic-containing electrospun scaffolds could function as a biologically safe antimicrobial drug delivery system for regenerative endodontics.
Department of Restorative Dentistry - Division of Dental Biomaterials.
M C
MC
Bottino
K
K
Kamocki
G H
GH
Yassen
J A
JA
Platt
M M
MM
Vail
Y
Y
Ehrlich
K J
KJ
Spolnik
R L
RL
Gregory
D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D018441:Biofilms / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002939:Ciprofloxacin / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects; D004058:Diffusion; D055119:Elastic Modulus; D013293:Enterococcus faecalis / Q000187:drug effects; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D008422:Materials Testing; D008795:Metronidazole / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry* / Q000633:toxicity; D016687:Polydioxanone / Q000737:chemistry / Q000633:toxicity; D016966:Porphyromonas gingivalis / Q000187:drug effects; D012390:Root Canal Therapy / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D013234:Stem Cells / Q000187:drug effects; D013314:Stress, Mechanical; D013499:Surface Properties; D013718:Tensile Strength; D013997:Time Factors; D054457:Tissue Scaffolds / Q000737:chemistry*
D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D008795:Metronidazole; D016687:Polydioxanone; D002939:Ciprofloxacin
disinfection; double antibiotic; electrospinning; nanofibers; regeneration; triple antibiotic