Nanotubes complexed with DNA and proteins for resistive-pulse sensing.
We use a resistive-pulse technique to analyze molecular hybrids of single-wall carbon nanotubes (SWNTs) wrapped in either single-stranded DNA or protein. Electric fields confined in a glass capillary nanopore allow us to probe the physical size and surface properties of molecular hybrids at the single-molecule level. We find that the translocation duration of a macromolecular hybrid is determined by its hydrodynamic size and solution mobility. The event current reveals the effects of ion exclusion by the rod-shaped hybrids and possible effects due to temporary polarization of the SWNT core. Our results pave the way to direct sensing of small DNA or protein molecules in a large unmodified solid-state nanopore by using nanofilaments as carriers.
School of Mechanical Engineering, Southeast University , Nanjing 210096, China.
Jingjie
J
Sha
Tawfique
T
Hasan
Silvia
S
Milana
Cristina
C
Bertulli
Nicholas A W
NA
Bell
Giulia
G
Privitera
Zhonghua
Z
Ni
Yunfei
Y
Chen
Francesco
F
Bonaccorso
Andrea C
AC
Ferrari
Ulrich F
UF
Keyser
Yan Yan S
YY
Huang
D004277:DNA, Single-Stranded / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D043942:Nanotubes / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
D004277:DNA, Single-Stranded; D011506:Proteins