Characterization of Cd-, Pb-, Zn-resistant endophytic Lasiodiplodia sp. MXSF31 from metal accumulating Portulaca oleracea and its potential in promoting the growth of rape in metal-contaminated soils.
The aim of this study was to characterize the features of a Cd-, Pb-, and Zn-resistant endophytic fungus Lasiodiplodia sp. MXSF31 and to investigate the potential of MXSF31 to remove metals from contaminated water and soils. The endophytic fungus was isolated from the stem of Portulaca oleracea growing in metal-contaminated soils. The maximum biosorption capacities of MXSF31 were 3.0 × 10(3), 1.1 × 10(4), and 1.3 × 10(4) mg kg(-1) for Cd, Pb, and Zn, respectively. The biosorption processes of Cd, Pb, and Zn by MXSF31 were well characterized with the pseudo-second-order kinetic model. The biosorption isotherm processes of Pb and Zn by the fungus were fitted better with the Langmuir model, while the biosorption processes of Cd was better fitted with the Freundlich model. The biosorption process of MXSF31 was attributed to the functional groups of hydroxyl, amino, carbonyl, and benzene ring on the cell wall. The active biomass of the strain removed more Cd, Pb, and Zn (4.6 × 10(4), 5.6 × 10(5), and 7.0 × 10(4) mg kg(-1), respectively) than the dead biomass. The inoculation of MXSF31 increased the biomass of rape (Brassica napus L.), the translocation factor of Cd, and the extraction amount of Cd by rape in the Cd+Pb-contaminated soils. The results indicated that the endophytic fungus strain had the potential to remove heavy metals from water and soils contaminated by multiple heavy metals, and plants accumulating multiple metals might harbor diverse fungi suitable for bioremediation of contaminated media.
Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, 510006, China. dengzujun66@163.com.
Zujun
Z
Deng
Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, 510275, China.
Renduo
R
Zhang
Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, 510275, China.
Yang
Y
Shi
Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, 510275, China.
Li'ao
L
Hu
School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.
Hongming
H
Tan
School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China. caolx@mail.sysu.edu.cn.
Lixiang
L
Cao
D000383:Agriculture; D001203:Ascomycota / Q000502:physiology*; D001673:Biodegradation, Environmental; D018533:Biomass; D029741:Brassica rapa / Q000382:microbiology / Q000502:physiology*; D002104:Cadmium / Q000378:metabolism; D007700:Kinetics; D007854:Lead / Q000378:metabolism; D019216:Metals, Heavy / Q000302:isolation & purification; D031822:Portulaca / Q000382:microbiology* / Q000502:physiology; D012987:Soil; D012989:Soil Pollutants / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism
D019216:Metals, Heavy; D012987:Soil; D012989:Soil Pollutants; D002104:Cadmium; D007854:Lead; D015032:Zinc