Manganese superoxide dismutase gene therapy protects against irradiation- induced intestinal injury.
Radiation-induced intestinal injury is a common complication in radiotherapy for solid organ malignancies in abdomen or pelvis. However, currently there are no approved medical countermeasures for radiation-induced intestinal injury. Therefore, it is urgent to develop new treatments for radiation-induced intestinal injury. In the present study, we demonstrated that bone marrow derived mesenchymal stem cells (MSCs) and overexpression of human manganese superoxide dismutase (MnSOD) could ameliorate radiation-induced intestinal syndrome. NOD/SCID mice received abdominal irradiation at a selected dose of 5 Gy, and then infused intravenously with MnSOD-MSCs. Mice body weight, survival and diarrhea were monitored for 30-days. Colonization and differentiation of MnSOD-MSCs in the irradiated intestine were analyzed by histological and immunohistochemical methods. Consequently, our data demonstrated that intravenous administration of MnSOD-MSCs improved survival, decreased diarrhea occurrence and protected the small intestinal structural integrity of irradiated mice. Moreover, intravenously transplanted MnSOD-MSCs could colonize the irradiated intestine and repair injured sites. These findings suggested that MnSOD-MSCs may be an attractive and potential option for radiation-induced intestinal injury.
Chao
C
Yang
Hai-Xu
HX
Chen
Yong
Y
Zhou
Min-Xia
MX
Liu
Yan
Y
Wang
Jie-Xi
JX
Wang
Su-Ping
SP
Ren
Ying
Y
Han
Beijing Institute of Transfusion Medicine, Beijing 100850, China. benyanwu@vip.sina.com.
Ben-Yan
BY
Wu
D050156:Adipogenesis; D000818:Animals; D002454:Cell Differentiation; D015316:Genetic Therapy; D006801:Humans; D007410:Intestinal Diseases / Q000209:etiology / Q000628:therapy*; D007421:Intestine, Small / Q000473:pathology / Q000528:radiation effects*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D010012:Osteogenesis; D010386:Pelvic Neoplasms / Q000532:radiotherapy; D011878:Radiotherapy / Q000009:adverse effects*; D013482:Superoxide Dismutase / Q000494:pharmacology*; D014161:Transduction, Genetic; D000099156:Superoxide Dismutase 2
D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2