A review on the electrochemical biosensors for determination of microRNAs.
MicroRNAs (miRNAs) are a family of non-protein-coding, endogenous, small RNAs. They are a group of gene regulators that function mainly by binding the 3' untranslated regions of specific target messenger RNA (mRNA) leading to gene inactivation by repression of mRNA transcription or induction of mRNA. Mature miRNAs are short molecules approximately 22 nucleotides in length. They regulate a wide range of biological functions from cell proliferation and death to cancer progression. Cellular miRNA expression levels can be used as biomarkers for the onset of disease states and in gene therapy for genetic disorders. Methods for detection of miRNA mainly include northern blotting, microarray, polymerase chain reaction (PCR). This review focuses on the use of electrochemical biosensors for the detection of microRNA.
Eletroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran; Università degli Studi di Firenze, Dipartimento di Chimica, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy. Electronic address: ehamidiasl@umz.ac.ir.
Ezat
E
Hamidi-Asl
Ilaria
I
Palchetti
Ehteram
E
Hasheminejad
Marco
M
Mascini
D015374:Biosensing Techniques / Q000706:statistics & numerical data*; D015152:Blotting, Northern; D016923:Cell Death; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D055664:Electrochemical Techniques / Q000706:statistics & numerical data*; D005057:Eukaryotic Cells / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D035683:MicroRNAs; D012333:RNA, Messenger
Biosensors; Electrochemistry; MicroRNAs