In silico prediction of conserved vaccine targets in Streptococcus agalactiae strains isolated from fish, cattle, and human samples.
Streptococcus agalactiae (Lancefield group B; group B streptococci) is a major pathogen that causes meningoencephalitis in fish, mastitis in cows, and neonatal sepsis and meningitis in humans. The available prophylactic measures for conserving human and animal health are not totally effective and have limitations. Effective vaccines against the different serotypes or genotypes of pathogenic strains from the various hosts would be useful. We used an in silico strategy to identify conserved vaccine candidates in 15 genomes of group B streptococci strains isolated from human, bovine, and fish samples. The degree of conservation, subcellular localization, and immunogenic potential of S. agalactiae proteins were investigated. We identified 36 antigenic proteins that were conserved in all 15 genomes. Among these proteins, 5 and 23 were shared only by human or fish strains, respectively. These potential vaccine targets may help develop effective vaccines that will help prevent S. agalactiae infection.
AQUAVET - Laboratório de Doenças de Animais Aquáticos, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
U P
UP
Pereira
S C
SC
Soares
J
J
Blom
C A G
CA
Leal
R T J
RT
Ramos
L C
LC
Guimarães
L C
LC
Oliveira
S S
SS
Almeida
S S
SS
Hassan
A R
AR
Santos
A
A
Miyoshi
A
A
Silva
A
A
Tauch
D
D
Barh
V
V
Azevedo
H C P
HC
Figueiredo
D000818:Animals; D002417:Cattle; D003198:Computer Simulation; D005260:Female; D005399:Fishes / Q000235:genetics*; D016680:Genome, Bacterial; D006801:Humans; D016233:Immunotherapy, Active; D008414:Mastitis, Bovine / Q000235:genetics / Q000382:microbiology / Q000517:prevention & control*; D058990:Molecular Targeted Therapy; D013290:Streptococcal Infections / Q000235:genetics / Q000276:immunology / Q000517:prevention & control* / Q000662:veterinary; D013292:Streptococcus agalactiae / Q000187:drug effects / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity