Biodegradation of 1,2,3,4-tetrachlorodibenzo-p-dioxin in liquid broth by brown-rot fungi.
Dioxins are a class of extremely hazardous molecules that might pose a threat to the environment. This work evaluated the microbial degradation of 1,2,3,4-tetrachlorodibenzo-p-dioxin (1,2,3,4-TCDD), in liquid broth using three brown-rot fungi and one white-rot fungi as control. A fast and reliable extraction method with recoveries of over 98% together with a validated GC-MS method was developed, and applied to quantify 1,2,3,4-TCDD in liquid broth, mycelia and reaction flask, with detection limits of 10 ppb. Among the four strains tested, brown-rot fungus Aspergillus aculeatus showed best results, removing up to 21% of dioxin after 30-day incubation. The results open both a path for biotechnological interest in bioremediation purposes and environmental behavior studies by using brown-rot fungus.
Department of Chemistry, Federal University of Sao Carlos, Rod. Washington Luiz, Km 235, 13.565-905 São Carlos, SP, Brazil.
Bruno
B
Perlatti
Maria Fátima das Graças Fernandes
MF
da Silva
João Batista
JB
Fernandes
Moacir Rossi
MR
Forim
D000704:Analysis of Variance; D001487:Basidiomycota / Q000378:metabolism*; D001673:Biodegradation, Environmental; D018533:Biomass; D003470:Culture Media / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D016014:Linear Models; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D015203:Reproducibility of Results
D003470:Culture Media; D000072317:Polychlorinated Dibenzodioxins; C092155:1,2,3,4-tetrachlorodibenzodioxin
Analytical validation; Biodegradation; Brown-rot fungus; Dioxins; Microorganisms