Human skin melanocyte migration towards stromal cell-derived factor-1α demonstrated by optical real-time cell mobility assay: modulation of their chemotactic ability by α-melanocyte-stimulating hormone.
To identify potential regulators of normal human melanocyte behaviour, we have developed an in vitro human melanocyte migration assay, using the optically accessible, real-time cell motility assay device TAXIScan. Coating of the glass surface with an extracellular matrix that served as scaffolding molecule was essential to demonstrate efficient melanocyte migration. Among several chemokines tested, stromal cell-derived factor (SDF)-1α/CXCL12 was the most effective driver of human normal skin melanocytes. Incubation of melanocytes with α-melanocyte-stimulating hormone (MSH) before the assay specifically enhanced CXCR4 expression and consequently chemotaxis towards SDF-1α/CXCL12. These results suggest that α-MSH acts on melanocytes to produce melanin as well as stimulates the cells to migrate to the site where they work through CXCR4 up-regulation, which is a new dynamic mode of action of α-MSH on melanocyte physiology.
Department of Biochemistry, Kawasaki Medical School, Kurashiki, Japan.
Akira
A
Yamauchi
Christophe
C
Hadjur
Tadahito
T
Takahashi
Itaru
I
Suzuki
Kunitaka
K
Hirose
Yann F
YF
Mahe
D002465:Cell Movement; D054377:Chemokine CXCL12 / Q000378:metabolism*; D018925:Chemokines / Q000378:metabolism; D002633:Chemotaxis; D003094:Collagen / Q000737:chemistry; D004338:Drug Combinations; D005109:Extracellular Matrix / Q000378:metabolism; D005786:Gene Expression Regulation; D005898:Glass / Q000737:chemistry; D006801:Humans; D007797:Laminin / Q000737:chemistry; D008543:Melanins / Q000737:chemistry; D008544:Melanocytes / Q000166:cytology*; D010766:Phosphorylation; D011509:Proteoglycans / Q000737:chemistry; D019718:Receptors, CXCR4 / Q000378:metabolism; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors; D015854:Up-Regulation; D000521:alpha-MSH / Q000378:metabolism*
C515255:CXCL12 protein, human; C527276:CXCR4 protein, human; D054377:Chemokine CXCL12; D018925:Chemokines; D004338:Drug Combinations; D007797:Laminin; D008543:Melanins; D011509:Proteoglycans; D019718:Receptors, CXCR4; C059485:matrigel; D000521:alpha-MSH; D003094:Collagen
SDF-1α/CXCL12; TAXIScan; chemotaxis; melanocyte; α-MSH