Ionizing radiation induced long-term alterations in the adult rat rostral migratory stream.
Ionizing radiation can induce significant injury to normal brain structures. To assess radiation-induced late effects, adult male Wistar rats received whole-body exposure with fractionated doses of gamma rays (a total dose of 4Gy) and were investigated thirty, sixty and ninety days later. Immunohistochemistry and confocal microscopy were used to determine the density of neuroblasts derived from the anterior subventricular zone (SVZa) and brain resident microglia distributed along and/or adjacent to subventricular zone-olfactory bulb axis (SVZ-OB axis). Cell counting was performed in four anatomical parts along the well defined pathway, known as the rostral migratory stream (RMS) represented by the SVZa, vertical arm, elbow and horizontal arm of the RMS. Strong overdistribution of neuroblasts was seen in the SVZa thirty and sixty days after irradiation replaced by a steep decline in the following parts of the RMS and the highest decrease ninety days after radiation treatment along the entire SVZ-OB axis. Radiation treatment led to a decline or loss of microglia in almost all counted parts through the entire experiment. Results showed that ultimate decline of the SVZa descendants and loss of microglia suggests a contributory role of reduced neurogenesis in the development of radiation-induced late effects.
Institute of Histology and Embryology, Jessenius Faculty of Medicine, Comenius University, Mala Hora 4, 03601 Martin, Slovak Republic. Electronic address: balentova@jfmed.uniba.sk.
Sona
S
Balentova
Department of Radiotherapy and Oncology, Martin University Hospital, Kollarova 2, 03659 Martin, Slovak Republic.
Eva
E
Hajtmanova
Jessenius Faculty of Medicine, Comenius University, Mala Hora 4, 03601 Martin, Slovak Republic.
Ria
R
Trylcova
Institute of Histology and Embryology, Jessenius Faculty of Medicine, Comenius University, Mala Hora 4, 03601 Martin, Slovak Republic.
Marian
M
Adamkov
Institute of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius University, Mala Hora 4, 03601 Martin, Slovak Republic.
Jan
J
Lehotsky
D000818:Animals; D039481:CD11b Antigen / Q000378:metabolism; D002465:Cell Movement; D002552:Cerebral Ventricles / Q000473:pathology / Q000528:radiation effects*; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D005720:Gamma Rays; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D055495:Neurogenesis; D009474:Neurons / Q000502:physiology / Q000528:radiation effects; D009479:Neuropeptides / Q000378:metabolism; D010641:Phenotype; D016548:Prosencephalon / Q000473:pathology / Q000528:radiation effects; D011833:Radiation Injuries, Experimental / Q000378:metabolism / Q000473:pathology*; D017208:Rats, Wistar
D039481:CD11b Antigen; C000714848:Dcx protein, rat; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides
CD11b; Doublecortin; Fractionated irradiation; Rat brain; SVZ–OB axis