Transplantation of neural tissue: quail-chick chimeras.
Tissue transplantation is an important approach in developmental neurobiology to determine cell fate, to uncover inductive interactions required for tissue specification and patterning as well as to establish tissue competence and commitment. Avian species are among the favorite model systems for these approaches because of their accessibility and relatively large size. Here we describe two culture techniques used to generate quail-chick chimeras at different embryonic stages and methods to distinguish graft and donor tissue.
Department of Craniofacial Development & Stem Cell Biology, King's College London, London, UK.
Andrea
A
Streit
Claudio D
CD
Stern
D000818:Animals; D001808:Blood Vessels / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism; D002642:Chick Embryo; D002678:Chimera / Q000196:embryology*; D054258:Neural Plate / Q000098:blood supply / Q000166:cytology; D054259:Neural Tube / Q000098:blood supply / Q000166:cytology; D010063:Ovum / Q000166:cytology; D011784:Quail / Q000196:embryology*; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology; D046509:Tissue Culture Techniques; D016707:Tissue Fixation; D016378:Tissue Transplantation / Q000295:instrumentation / Q000379:methods*