Programmed cell death in a patient with hepatocellular carcinoma treated with yttrium-90 and doxorubicin-loaded beads.
Molecular analysis of apoptosis and autophagy pathways was performed from a single hepatocellular carcinoma treated with yttrium-90 and doxorubicin-loaded beads before resection and compared with normal liver tissue from the margins. Both bead formulations activated apoptosis-associated mechanisms and increased autophagy pathway protein levels. Increased DNA fragmentation and autophagy markers were seen in tumor treated with drug-eluting beads compared with yttrium-90-treated tumor. These results suggest that both microembolic therapies activate cell death signaling, although differences in apoptosis and autophagy pathways were seen in this patient. Knowledge of mechanisms of action for each treatment may enhance future therapeutic strategies.
Robert S. Dow Neurobiology Laboratories, Portland, Oregon; Cancer Research Laboratories, Portland, Oregon; Department of Neurobiology, Morehouse School of Medicine, Atlanta, Georgia.
Robert
R
Meller
Lorena
L
Galvan
Jing Quan
JQ
Lan
Esther
E
Han
Jason
J
Bauer
Katherine T
KT
Morris
D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D001343:Autophagy / Q000187:drug effects / Q000528:radiation effects; D002214:Capsules; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000628:therapy*; D059248:Chemoradiotherapy / Q000379:methods; D003692:Delayed-Action Preparations / Q000008:administration & dosage; D004317:Doxorubicin / Q000008:administration & dosage*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000628:therapy*; D008297:Male; D008875:Middle Aged; D019275:Radiopharmaceuticals / Q000627:therapeutic use; D016896:Treatment Outcome; D015021:Yttrium Radioisotopes / Q000627:therapeutic use*
D051017:Apoptosis Regulatory Proteins; D002214:Capsules; D003692:Delayed-Action Preparations; D019275:Radiopharmaceuticals; D015021:Yttrium Radioisotopes; D004317:Doxorubicin
(90)Y; DEBT; HCC; TUNEL; drug-eluting bead therapy; hepatocellular carcinoma; terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; yttrium-90