2,4-Di-tert-butylphenol from sweet potato protects against oxidative stress in PC12 cells and in mice.
In this study, the protective effect of sweet potato extract against hydrogen peroxide-induced oxidative stress and cytotoxicity on the pheochromocytoma cell line (PC12) was investigated. The active component of the sweet potato extract was purified and determined to be 2,4-di-tert-butylphenol. The antioxidant capacity of 2,4-di-tert-butylphenol was measured by using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical. To examine the effects of 2,4-di-tert-butylphenol on amyloid-beta peptide (Aβ1₁₋₄₂)-induced learning and memory impairment in mice, in vivo behavioral tests were performed. Administration of 2,4-di-tert-butylphenol increased alternation behavior in mice injected with Aβ₁₋₄₂. These results suggest that sweet potato extract could be protective against Aβ-induced neurotoxicity, possibly due to the antioxidative capacity of its constituent, 2,4-di-tert-butylphenol.
1 Department of Food Technology, Korea University , Seoul, Republic of Korea.
Soo Jung
SJ
Choi
Jae Kyeom
JK
Kim
Hye Kyung
HK
Kim
Keith
K
Harris
Chang-Ju
CJ
Kim
Gwi Gun
GG
Park
Cheung-Seog
CS
Park
Dong-Hoon
DH
Shin
D000544:Alzheimer Disease / Q000188:drug therapy / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000009:adverse effects / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D052160:Benzothiazoles / Q000378:metabolism; D003072:Cognition Disorders / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D027723:Ipomoea batatas / Q000737:chemistry*; D029561:Lythraceae / Q000737:chemistry*; D008297:Male; D008569:Memory Disorders / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D010636:Phenols / Q000494:pharmacology* / Q000627:therapeutic use; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D013451:Sulfonic Acids / Q000378:metabolism
D016229:Amyloid beta-Peptides; D000975:Antioxidants; D052160:Benzothiazoles; D010636:Phenols; D010936:Plant Extracts; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C056559:2,4-di-tert-butylphenol