Taishan Pinus massoniana pollen polysaccharides promote immune responses of recombinant Bordetella avium ompA in BALB/c mice.
To study the effects of Taishan Pinus massoniana pollen polysaccharides (TPPPS) on Bordetella avium outer membrane protein A (ompA) recombinant protein vaccine, ompA was expressed, confirmed by Western blotting and mixed with TPPPS. Female BALB/c mice were randomly divided into six groups (I-VI). Groups I, II, and III were treated with TPPPS-ompA at doses of 200, 400, and 800 mg/ml, respectively. Groups IV, V, and VI were treated with Freund's adjuvant-ompA, pure ompA, and physiological saline, respectively. On days 3, 7, 14, 21, 28, 35, 42, and 49 after the first vaccination, antibody titers, interleukin-2 (IL-2) levels, peripheral blood CD4+ and CD8+ levels, and T lymphocyte proliferation rates in peripheral blood, as well as secreting-type immunoglobulin A (SIgA) levels in the duodenum, were measured. The antibody titers against ompA, IL-2, T lymphocyte proliferation rate, CD4+, and CD8+ in Group II were significantly (P<0.05) higher than those in other groups. However, little difference in SIgA content was observed among Groups I, II, and IV. These results indicated that TPPPS strengthened humoral and cellular immune response against recombinant ompA vaccine and 400 mg/ml TPPPS showed significance (P<0.05) compared with Freund's adjuvant. Therefore, TPPPS can be developed into an adjuvant for recombinant protein vaccines or plant-derived medicine for animal husbandry.
College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong 271018, PR China.
Xue
X
Zhao
Manfei
M
Liang
PingPing
P
Yang
FanXia
F
Guo
DeQin
D
Pan
Xuan
X
Huang
Yifu
Y
Li
Cun
C
Wu
Tinghe
T
Qu
RuiLiang
R
Zhu
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology* / Q000302:isolation & purification; D001428:Bacterial Vaccines; D042482:Bordetella avium / Q000276:immunology*; D049109:Cell Proliferation; D005260:Female; D007070:Immunoglobulin A / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D028223:Pinus / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D011134:Polysaccharides / Q000494:pharmacology*; D011994:Recombinant Proteins; D013601:T-Lymphocytes / Q000276:immunology; D014614:Vaccines, Synthetic
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D007070:Immunoglobulin A; D007376:Interleukin-2; D011134:Polysaccharides; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; C078167:OMPA outer membrane proteins
ASP; Adjuvant; Astragalus polysaccharides; B.avium ompA; Bordetella avium; Bordetella avium outer membrane protein A; Immune response; OD; Polysaccharide; Prokaryotic expression; SIgA; TPPPS; Taishan Pinus massoniana pollen polysaccharides; optical density; secreting-type immunoglobulin A