Hydrogen peroxide production and free radical-mediated cell stress in Mycoplasma bovis pneumonia.
Mycoplasma bovis causes chronic pneumonia and polyarthritis in feedlot cattle. M. bovis infects the lungs of most feedlot cattle, but the majority of calves never develop disease. Competing explanations are that some strains of M. bovis are more virulent than others or, alternatively, that calves require some other abnormality to be present in order for M. bovis to cause disease. We hypothesize that H2O2 production is an important virulence factor of M. bovis, causing oxidative injury to lung tissue. A second hypothesis is that isolates associated with caseonecrotic bronchopneumonia have an increased capacity for H2O2 production. Immunohistochemical markers of oxidative stress (4-hydroxynonenal, HN) and nitrative stress (3-nitrotyrosine, NT) were compared in lungs of calves with caseonecrotic bronchopneumonia characteristic of M. bovis infection, with other forms of bronchopneumonia or with non-inflamed lungs. HN and NT were identified in M. bovis pneumonia, mainly in foci of caseous necrosis. HN was not observed in inflamed non-necrotic tissue in lesions typical of pneumonic pasteurellosis. H2O2 production by M. bovis was identified, but the levels did not differ in isolates from calves with caseonecrotic bronchopneumonia compared with those with non-inflamed lungs or other forms of pneumonia. These findings provide evidence that oxidative and nitrative injury contribute to the formation of the caseonecrotic lesions that are characteristic of M. bovis pneumonia and that production of H2O2 by M. bovis may contribute to this oxidative injury.
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
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C
Schott
Animal Health Laboratory, Laboratory Services Division, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
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H
Cai
Animal Health Laboratory, Laboratory Services Division, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
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L
Parker
Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
K G
KG
Bateman
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada. Electronic address: jcaswell@uoguelph.ca.
J L
JL
Caswell
D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism* / Q000473:pathology; D005609:Free Radicals / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008168:Lung / Q000378:metabolism / Q000473:pathology; D009175:Mycoplasma Infections / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D045682:Mycoplasma bovis / Q000302:isolation & purification*; D018384:Oxidative Stress / Q000502:physiology*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D000447:Aldehydes; D005609:Free Radicals; C002744:3-nitrotyrosine; D014443:Tyrosine; D006861:Hydrogen Peroxide; C027576:4-hydroxy-2-nonenal
Mycoplasma bovis; nitric oxide; oxidative stress; pneumonia