Non-invasive measurement of cerebral oxygen metabolism in the mouse brain by ultra-high field (17)O MR spectroscopy.
To assess cerebral energetics in transgenic mouse models of neurologic disease, a robust, efficient, and practical method for quantification of cerebral oxygen consumption is needed. (17)O magnetic resonance spectroscopy (MRS) has been validated to measure cerebral metabolic rate of oxygen (CMRO2) in the rat brain; however, mice present unique challenges because of their small size. We show that CMRO2 measurements with (17)O MRS in the mouse brain are highly reproducible using 16.4 Tesla and a newly designed oxygen delivery system. The method can be utilized to measure mitochondrial function in mice quickly and repeatedly, without oral intubation, and has numerous potential applications to study cerebral energetics.
Center for MR Research, Department of Radiology, Medical School, University of Minnesota, Minneapolis, Minnesota, USA.
Weina
W
Cui
Xiao-Hong
XH
Zhu
Manda L
ML
Vollmers
Emily T
ET
Colonna
Gregor
G
Adriany
Brandon
B
Tramm
Janet M
JM
Dubinsky
Gülin
G
Öz
D000818:Animals; D001921:Brain / Q000378:metabolism*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption; D010103:Oxygen Isotopes / Q000378:metabolism
D010103:Oxygen Isotopes; D010100:Oxygen