Caspases and p38 MAPK regulate endothelial cell adhesiveness for mesenchymal stem cells.
Mesenchymal stem cells natively circulating or delivered into the blood stream home to sites of injury. The mechanism of mesenchymal stem cell homing to sites of injury is poorly understood. We have shown that the development of apoptosis in endothelial cells stimulates endothelial cell adhesiveness for mesenchymal stem cells. Adhesion of mesenchymal stem cells to apoptotic endothelial cells depends on the activation of endothelial caspases and p38 MAPK. Activation of p38 MAPK in endothelial cells has a primary effect while the activation of caspases potentiates the mesenchymal stem cell adhesion. Overall, our study of the mesenchymal stem cell interaction with endothelial cells indicates that mesenchymal stem cells recognize and specifically adhere to distressed/apoptotic endothelial cells.
Department of Physiology and Biophysics, Institute of Molecular Cardiology, Stony Brook University, Stony Brook, New York, United States of America.
Irina A
IA
Potapova
Ira S
IS
Cohen
Sergey V
SV
Doronin
D061945:Caspase Inhibitors / Q000494:pharmacology; D020169:Caspases / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008084:Lipoxygenase / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D054467:Phospholipases A2 / Q000378:metabolism; D010766:Phosphorylation; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D061945:Caspase Inhibitors; D008084:Lipoxygenase; D048051:p38 Mitogen-Activated Protein Kinases; D054467:Phospholipases A2; D020169:Caspases