A simple and reliable method for distinguishing danshen in salvia: simultaneous quantification of six active compositions by HPLC.
A simple and reliable method for distinguishing Danshen is important to evaluate the quality and clinical efficiency of these species. An HPLC method was developed for the determination of protocatechuic aldehyde, salvianolic acid A, salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone IIA in 23 samples of Salvia. The analytes were separated on an Agilent XDB C18 reversed-phase column coupled with a Phenomenex C18 guard column using a gradient elution of acetonitile-0.1% aqueous phosphoric acid as the mobile phase at a flow rate 0.8 mL/min and UV detection at 280 nm. The method allowing the simultaneous quantification of six major active compositions was optimized and validated for linearity, precision, accuracy and limits of detection (LOD) and quantification. The LOD ranged from 0.019 to 0.850 µg/mL (R(2) ≥ 0.9998). Accuracy, precision and reproducibility were all within the required limits. The average recovery between 96.49 and 102.16% and the relative standard deviations were <3.01%. Based on the six compositions content and clustering result, this research results suggest that these six major active compositions could be distinguishing markers for Danshen and non-Danshen.
College of Biology and Science, Sichuan Agricultural University, Ya'an 625014, China.
Tao
T
Wang
College of Biology and Science, Sichuan Agricultural University, Ya'an 625014, China.
Hui
H
Zhang
College of Biology and Science, Sichuan Agricultural University, Ya'an 625014, China.
Long
L
Wang
College of Biology and Science, Sichuan Agricultural University, Ya'an 625014, China.
Yuanyuan
Y
Jiang
College of Biology and Science, Sichuan Agricultural University, Ya'an 625014, China zhang8434@sina.com.
Li
L
Zhang
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China.
Yonghong
Y
Zhou
College of Biology and Science, Sichuan Agricultural University, Ya'an 625014, China.
Ruiwu
R
Yang
College of Biology and Science, Sichuan Agricultural University, Ya'an 625014, China.
Chunbang
C
Ding
College of Biology and Science, Sichuan Agricultural University, Ya'an 625014, China.
Xiaoli
X
Wang
D045784:Abietanes / Q000032:analysis; D001572:Benzofurans / Q000032:analysis; D002109:Caffeic Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D007773:Lactates / Q000032:analysis; D057230:Limit of Detection; D010616:Phenanthrenes / Q000032:analysis; D015203:Reproducibility of Results; D027544:Salvia / Q000737:chemistry; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D053630:Depsides
D045784:Abietanes; D001572:Benzofurans; D002109:Caffeic Acids; D004365:Drugs, Chinese Herbal; D007773:Lactates; D010616:Phenanthrenes; C021751:tanshinone; C028972:dan-shen root extract; C066201:salvianolic acid A; C037886:cryptotanshinone; C076944:salvianolic acid B; D053630:Depsides