Cell type-specific transcriptome of Brassicaceae stigmatic papilla cells from a combination of laser microdissection and RNA sequencing.
Pollination is an early and critical step in plant reproduction, leading to successful fertilization. It consists of many sequential processes, including adhesion of pollen grains onto the surface of stigmatic papilla cells, foot formation to strengthen pollen-stigma interaction, pollen hydration and germination, and pollen tube elongation and penetration. We have focused on an examination of the expressed genes in papilla cells, to increase understanding of the molecular systems of pollination. From three representative species of Brassicaceae (Arabidopsis thaliana, A. halleri and Brassica rapa), stigmatic papilla cells were isolated precisely by laser microdissection, and cell type-specific gene expression in papilla cells was determined by RNA sequencing. As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species. Among the17,240 genes predicted in A. thaliana, one-third were papilla specific while approximately half of the genes were detected in all tissues examined. Bioinformatics analysis revealed that genes related to a wide range of reproduction and development functions are expressed in papilla cells, particularly metabolism, transcription and membrane-mediated information exchange. These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells. This study provides fundamental biological knowledge to dissect the molecular mechanisms of pollination in papilla cells and will shed light on our understanding of plant reproduction mechanisms.
Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan.
Masaaki
M
Osaka
Tomoki
T
Matsuda
Satomi
S
Sakazono
Hiromi
H
Masuko-Suzuki
Shunsuke
S
Maeda
Misato
M
Sewaki
Mikako
M
Sone
Hirokazu
H
Takahashi
Mikio
M
Nakazono
Megumi
M
Iwano
Seiji
S
Takayama
Kentaro K
KK
Shimizu
Kentaro
K
Yano
Yong Pyo
YP
Lim
Go
G
Suzuki
Keita
K
Suwabe
Masao
M
Watanabe
D017360:Arabidopsis / Q000166:cytology / Q000235:genetics*; D001483:Base Sequence; D029741:Brassica rapa / Q000166:cytology / Q000235:genetics*; D019295:Computational Biology; D017403:In Situ Hybridization; D042282:Microdissection / Q000379:methods*; D009928:Organ Specificity; D016612:Paraffin Embedding; D010940:Plant Proteins / Q000235:genetics; D011058:Pollen / Q000166:cytology / Q000235:genetics; D053205:Pollen Tube / Q000166:cytology / Q000235:genetics; D054817:Pollination / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D012098:Reproduction; D017423:Sequence Analysis, RNA / Q000379:methods*; D013045:Species Specificity; D059467:Transcriptome
D010940:Plant Proteins; D018749:RNA, Plant
Arabidopsis halleri; Arabidopsis thaliana; Brassica rapa; Laser microdissection; Papilla cell; RNA sequencing