[Simultaneous determination of paeoniflorin, ferulic acid, prim-O-glucosylcimifugin and 4'-O-beta-glucopyranosyl-5-O-methylvisamminol in zhengtian pills by HPLC].
To simultaneously determine paeoniflorin, ferulic acid, prim-O-glucosylcimifugin and 4'-O-beta-glucopyranosyl-5-O-methylvisamminol in Zhengtian pills. In the test, Insertil ODS-C18 column (4.6 mm x 250 mm, 5 microm) was adopted, with acetonitrile-0.05% phosphoric acid solution as the mobile phase for gradient elution. The flow rate was 1.0 mL x min(-1), the column temperature was 30 degrees C and the detection wavelength was 230 nm. According to the results of the test, paeoniflorin, ferulic acid, prim-O-glucosylcimifugin and 4'-O-beta-glucopyranosyl-5-O-methylvisamminol showed good linear relations between peak areas and sample sizes in 11.37-170.5, 2.188-32.82, 2.896-43.44, and 3.000-45.00 mg x L(-1) (r = 0.999 9, 0.999 9, 1.000 0, 1.000 0), respectively. The average recoveries (n = 6) were 102.3% (RSD 1.2%), 99.71% (RSD 1.9%), 101.2% (RSD 1.2%), and 99.40% (RSD 2.4%), respectively. The above four components were determined in five batches of samples by using the established method, with satisfactory results. The method was so simple, accurate and highly reproducible that it could be used for quality control of the four components in Zhengtian pills.
Xiamen Institute for Drugs Control, Xiamen 361012, China. huanglan7902@163.com
Lan
L
Huang
Hui-Ling
HL
Chen
Ling-Ling
LL
Li
D001565:Benzoates / Q000032:analysis*; D001952:Bridged-Ring Compounds / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000592:standards; D005960:Glucosides / Q000032:analysis*; D009005:Monosaccharides / Q000032:analysis*; D039821:Monoterpenes; D011786:Quality Control; D014966:Xanthenes / Q000032:analysis*; D006027:Glycosides
D001565:Benzoates; D001952:Bridged-Ring Compounds; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D009005:Monosaccharides; D039821:Monoterpenes; D014966:Xanthenes; C498945:prim-O-glucosylcimifugin; C015423:peoniflorin; D006027:Glycosides; C004999:ferulic acid