Abscisic acid suppresses the highly occurred somatic homologous recombination in Arabidopsis rfc1 mutant.
The phytohormone abscisic acid (ABA) regulates many aspects of plant growth, including seed germination, root growth and cell division. Previous study indicates that ABA treatment increases DNA damage and somatic homologous recombination (HR) in Arabidopsis abo4/pol ɛ (aba overly-sensitive 4 /DNA polymerase ɛ) mutants. DNA replication factor C (RFC) complex is required for loading PCNA (Proliferating Cell Nuclear Antigen) during DNA replication. The defect in RFC1, the largest subunit of RFC, causes the high HR and DNA damage sensitivity in Arabidopsis. Here we found that like pol ε/abo4, rfc1 is sensitive to ABA in both ABA-inhibiting seed germination and root growth. However, ABA treatment greatly reduces HR and also reduces the expression of the DNA-damaged marker genes in rfc1. These results suggest that RFC1 plays critical roles in ABA-mediated HR in Arabidopsis.
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Tingxiu
T
Yao
Dan
D
Jin
Qian
Q
Liu
Zhizhong
Z
Gong
D000040:Abscisic Acid / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D019926:Cyclin B / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017930:Genes, Reporter; D018525:Germination / Q000187:drug effects; D059765:Homologous Recombination / Q000187:drug effects*; D009154:Mutation; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D051818:Replication Protein C / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects
D029681:Arabidopsis Proteins; C462528:CycB1 protein, Arabidopsis; D019926:Cyclin B; D010937:Plant Growth Regulators; C587946:RFC1 protein, Arabidopsis; D000040:Abscisic Acid; D051818:Replication Protein C
Abscisic acid; DNA replication factor C1; Genomic stability