Distinct tRNA modifications in the thermo-acidophilic archaeon, Thermoplasma acidophilum.
Thermoplasma acidophilum is a thermo-acidophilic archaeon. We purified tRNA(Leu) (UAG) from T. acidophilum using a solid-phase DNA probe method and determined the RNA sequence after determining via nucleoside analysis and m(7)G-specific aniline cleavage because it has been reported that T. acidophilum tRNA contains m(7)G, which is generally not found in archaeal tRNAs. RNA sequencing and liquid chromatography-mass spectrometry revealed that the m(7)G modification exists at a novel position 49. Furthermore, we found several distinct modifications, which have not previously been found in archaeal tRNA, such as 4-thiouridine9, archaeosine13 and 5-carbamoylmethyuridine34. The related tRNA modification enzymes and their genes are discussed.
Department of Materials Science and Biotechnology, Graduate School of Science and Engineering, Ehime University, Bunkyo 3, Matsuyama, Ehime 790-8577, Japan.
Chie
C
Tomikawa
Takayuki
T
Ohira
Yasushi
Y
Inoue
Takuya
T
Kawamura
Akihiko
A
Yamagishi
Tsutomu
T
Suzuki
Hiroyuki
H
Hori
D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000378:metabolism; D001483:Base Sequence; D006151:Guanosine / Q000737:chemistry; D013058:Mass Spectrometry; D009690:Nucleic Acid Conformation; D012343:RNA, Transfer / Q000737:chemistry*; D013822:Thermoplasma / Q000235:genetics / Q000378:metabolism*
D019843:Archaeal Proteins; D006151:Guanosine; D012343:RNA, Transfer
4-Thiouridine; 5-Carbamoylmethyuridine; 7-Methylguanine; Archaea; Archaeosine; tRNA