Effect of Emdogain enamel matrix derivative and BMP-2 on the gene expression and mineralized nodule formation of alveolar bone proper-derived stem/progenitor cells.
The objective of this study was to evaluate the effect of Emdogain (Enamel Matrix Derivative, EMD) and Bone Morphogenetic Protein-2 (BMP-2), either solely or in combination, on the gene expression and mineralized nodule formation of alveolar bone proper-derived stem/progenitor cells. Stem/progenitor cells were isolated from human alveolar bone proper, magnetically sorted using STRO-1 antibodies, characterized flowcytometrically for their surface markers' expression, and examined for colony formation and multilineage differentiation potential. Subsequently, cells were treated over three weeks with 100 μg/ml Emdogain (EMD-Group), or 100 ng/ml BMP-2 (BMP-Group), or a combination of 100 ng/ml BMP-2 and 100 μg/ml Emdogain (BMP/EMD-Group). Unstimulated stem/progenitor cells (MACS(+)-Group) and osteoblasts (OB-Group) served as controls. Osteogenic gene expression was analyzed using RTq-PCR after 1, 2 and 3 weeks (N = 3/group). Mineralized nodule formation was evaluated by Alizarin-Red staining. BMP and EMD up-regulated the osteogenic gene expression. The BMP Group showed significantly higher expression of Collagen-I, III, and V, Alkaline phosphatase and Osteonectin compared to MACS(+)- and OB-Group (p < 0.05; Two-way ANOVA/Bonferroni) with no mineralized nodule formation. Under in-vitro conditions, Emdogain and BMP-2 up-regulate the osteogenic gene expression of stem/progenitor cells. The combination of BMP-2 and Emdogain showed no additive effect and would not be recommended for a combined clinical stimulation.
Clinic for Conservative Dentistry and Periodontology (Head: Prof. Dr. C. Dörfer), School of Dental Medicine, Christian Albrechts-University of Kiel, Arnold-Heller-Str. 3, Haus 26, 24105 Kiel, Germany; Oral Medicine and Periodontology Department, Faculty of Oral and Dental Medicine, Cairo University, Egypt. Electronic address: karim.fawzy@gmail.com.
Karim M
KM
Fawzy El-Sayed
Clinic for Conservative Dentistry and Periodontology (Head: Prof. Dr. C. Dörfer), School of Dental Medicine, Christian Albrechts-University of Kiel, Arnold-Heller-Str. 3, Haus 26, 24105 Kiel, Germany.
Christof
C
Dörfer
Clinic for Applied Cellular Therapy, Christian Albrechts-University of Kiel, Kiel, Germany.
Hendrick
H
Ungefroren
Department of Clinical Pathology, Faculty of Medicine, Cairo University, Egypt.
Neemat
N
Kassem
Department of Oral and Maxillofacial Surgery, Christian Albrechts-University of Kiel, Kiel, Germany.
Jörg
J
Wiltfang
Department of Operative and Preventive Dentistry, Charité - Universitätsmedizin Berlin, Berlin, Germany.
Sebastian
S
Paris
D000469:Alkaline Phosphatase / Q000187:drug effects; D000539:Alveolar Process / Q000166:cytology* / Q000187:drug effects; D000880:Anthraquinones; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D002113:Calcification, Physiologic / Q000187:drug effects*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000187:drug effects; D024061:Collagen Type III / Q000187:drug effects; D024062:Collagen Type V / Q000187:drug effects; D004396:Coloring Agents; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D010006:Osteoblasts / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics; D015676:Osteonectin / Q000187:drug effects; D013234:Stem Cells / Q000187:drug effects*; D013997:Time Factors; D015854:Up-Regulation
D000880:Anthraquinones; C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D024042:Collagen Type I; D024061:Collagen Type III; D024062:Collagen Type V; D004396:Coloring Agents; D003746:Dental Enamel Proteins; D015676:Osteonectin; C109069:enamel matrix proteins; C010078:alizarin; D000469:Alkaline Phosphatase
Alveolar bone proper; BMP-2; EMD; Stem cells; Tissue engineering