MiR-223 is dispensable for platelet production and function in mice.
MicroRNAs (miRNAs) are key physiological regulators in multiple cell types. Here, we assessed platelet production and function in mice deficient in miR-223, one of the most abundantly expressed miRNAs in platelets and megakaryocytes. We found platelet number, size, life-span as well as surface expression of platelet adhesion receptors to be unchanged in miR-223-deficient mice. Likewise, loss of miR-223 did not affect platelet activation, adhesion and aggregation and also had no effect on bleeding times. Moreover, miR-223 null megakaryocytes developed normally and were capable to form pro-platelets. However, we detected a transient delay in the recovery of platelet numbers following antibody-induced platelet depletion in miR-223-deficient animals. This delay was not observed after transplantation of bone marrow from miR-223-deficient animals into wild-type recipients, indicating a non-cell-autonomous role of miR-223 for thrombopoiesis. Overall, our data indicate a surprisingly modest role of miR-223 in platelet production, while the function of platelets does not seem to depend on miR-223.
Stefan Engelhardt, MD, PhD, Institut für Pharmakologie und Toxikologie, Technische Universität München, Biedersteiner Strasse 29, 80802 Munich, Germany, Tel.: +49 89 4140 3260, E-mail: stefan.engelhardt@tum.de.
Simon
S
Leierseder
Tobias
T
Petzold
Lin
L
Zhang
Xavier
X
Loyer
Steffen
S
Massberg
Stefan
S
Engelhardt
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000235:genetics; D001760:Bleeding Time; D001777:Blood Coagulation / Q000235:genetics; D001792:Blood Platelets / Q000502:physiology*; D016026:Bone Marrow Transplantation; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D002452:Cell Count; D002478:Cells, Cultured; D006801:Humans; D008533:Megakaryocytes / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D015539:Platelet Activation / Q000235:genetics; D034061:Thrombopoiesis / Q000235:genetics; D018183:Transplantation Chimera
D015815:Cell Adhesion Molecules; C544877:MIRN223 microRNA, mouse; D035683:MicroRNAs
MicroRNA; megakaryocytes; platelets; transgenic mice