Evaluation of tissue PCA3 expression in prostate cancer by RNA in situ hybridization--a correlative study with urine PCA3 and TMPRSS2-ERG.
PCA3 is a prostate-specific non-coding RNA, with utility as a urine-based early detection biomarker. Here, we report the evaluation of tissue PCA3 expression by RNA in situ hybridization in a cohort of 41 mapped prostatectomy specimens. We compared tissue PCA3 expression with tissue level ERG expression and matched pre-prostatectomy urine PCA3 and TMPRSS2-ERG levels. Across 136 slides containing 138 foci of prostate cancer, PCA3 was expressed in 55% of cancer foci and 71% of high-grade prostatic intraepithelial neoplasia foci. Overall, the specificity of tissue PCA3 was >90% for prostate cancer and high-grade prostatic intraepithelial neoplasia combined. Tissue PCA3 cancer expression was not significantly associated with urine PCA3 expression. PCA3 and ERG positivity in cancer foci was positively associated (P<0.01). We report the first comprehensive assessment of PCA3 expression in prostatectomy specimens, and find limited correlation between tissue PCA3 and matched urine in prostate cancer.
Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA.
Joshua I
JI
Warrick
1] Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA [2] Michigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI, USA [3] Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI, USA [4] Department of Urology, University of Michigan Medical School, Ann Arbor, MI, USA.
Scott A
SA
Tomlins
1] Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA [2] Michigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI, USA.
Shannon L
SL
Carskadon
Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA.
Allison M
AM
Young
1] Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA [2] Michigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI, USA.
Javed
J
Siddiqui
Department of Urology, University of Michigan Medical School, Ann Arbor, MI, USA.
John T
JT
Wei
1] Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA [2] Michigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI, USA [3] Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI, USA [4] Department of Urology, University of Michigan Medical School, Ann Arbor, MI, USA [5] Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor, MI, USA.
Arul M
AM
Chinnaiyan
1] Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA [2] Michigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI, USA [3] Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI, USA.
Lakshmi P
LP
Kunju
1] Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA [2] Michigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI, USA [3] Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI, USA.
Nallasivam
0000000206339772
N
Palanisamy
D000951:Antigens, Neoplasm / Q000235:genetics* / Q000652:urine; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000652:urine; D055088:Early Detection of Cancer; D015321:Gene Rearrangement; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization / Q000379:methods*; D008297:Male; D015514:Oncogene Proteins, Fusion / Q000235:genetics* / Q000652:urine; D011237:Predictive Value of Tests; D011468:Prostatectomy; D019048:Prostatic Intraepithelial Neoplasia / Q000235:genetics* / Q000473:pathology / Q000601:surgery / Q000652:urine; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery / Q000652:urine; D022661:RNA, Untranslated / Q000235:genetics* / Q000652:urine
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D015514:Oncogene Proteins, Fusion; D022661:RNA, Untranslated; C515553:TMPRSS2-ERG fusion protein, human; C479341:prostate cancer antigen 3, human