Aminopeptidase N (CD13) is involved in phagocytic processes in human dendritic cells and macrophages.
Aminopeptidase N (APN or CD13) is a membrane ectopeptidase expressed by many cell types, including myelomonocytic lineage cells: monocytes, macrophages, and dendritic cells. CD13 is known to regulate the biological activity of various peptides by proteolysis, and it has been proposed that CD13 also participates in several functions such as angiogenesis, cell adhesion, metastasis, and tumor invasion. We had previously reported that, in human monocytes and macrophages, CD13 modulates the phagocytosis mediated by receptors for the Fc portion of IgG antibodies (FcγRs). In this work, we analyzed the possible interaction of CD13 with other phagocytic receptors. We found out that the cross-linking of CD13 positively modulates the phagocytosis mediated by receptors of the innate immune system, since a significant increase in the phagocytosis of zymosan particles or heat-killed E. coli was observed when CD13 was cross-linked using anti-CD13 antibodies, in both macrophages and dendritic cells. Also, we observed that, during the phagocytosis of zymosan, CD13 redistributes and is internalized into the phagosome. These findings suggest that, besides its known functions, CD13 participates in phagocytic processes in dendritic cells and macrophages.
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado Postal 70228, Ciudad Universitaria, 04510 México, DF, Mexico.
Mónica I
MI
Villaseñor-Cardoso
Dulce A
DA
Frausto-Del-Río
Enrique
E
Ortega
D018826:CD13 Antigens / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D004705:Endocytosis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D009000:Monocytes / Q000166:cytology; D010586:Phagocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D010641:Phenotype; D021381:Protein Transport / Q000187:drug effects; D051195:Toll-Like Receptor 2 / Q000378:metabolism; D015054:Zymosan / Q000378:metabolism
D003432:Cross-Linking Reagents; D016207:Cytokines; C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2; D015054:Zymosan; D018826:CD13 Antigens