microR-142-3p down-regulates IRAK-1 in response to Mycobacterium bovis BCG infection in macrophages.
MicroRNAs (miRNAs) have been demonstrated to play a pivotal role in the regulation of target gene expression at the post-transcriptional level. In order to better understand the role of miRNA in the immunological regulation of macrophages against Mycobacterium bovis BCG infection, we explored the alteration of immune-related miRNA profile in macrophage RAW264.7 cells in response to BCG infection in this study. Our results demonstrated that miR-142-3p was a potential to negatively regulate the production of pro-inflammatory mediators NF-κB (NF-κB1), TNF-α and IL-6 in the macrophages in part through a mechanism of targeting IRAK-1 gene and post-transcriptionally down-regulating IRAK-1 protein expression.
General Hospital of Ningxia Medical University, Yinchuan 750004, China; School of Laboratory Medicine, Ningxia Medical University, Yinchuan 750004, China. Electronic address: guangxianxu2004@yahoo.com.cn.
Guangxian
G
Xu
Zhaobo
Z
Zhang
Jun
J
Wei
Yilin
Y
Zhang
Ying
Y
Zhang
Le
L
Guo
Xiaoming
X
Liu
D000818:Animals; D002478:Cells, Cultured; D015536:Down-Regulation / Q000235:genetics / Q000276:immunology; D007113:Immunity, Innate / Q000235:genetics / Q000276:immunology; D018836:Inflammation Mediators / Q000378:metabolism; D053592:Interleukin-1 Receptor-Associated Kinases / Q000096:biosynthesis* / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D008264:Macrophages / Q000276:immunology / Q000382:microbiology*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009163:Mycobacterium bovis; D016328:NF-kappa B / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014162:Transfection; D014376:Tuberculosis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
D018836:Inflammation Mediators; D015850:Interleukin-6; D035683:MicroRNAs; C529102:Mirn142 microRNA, mouse; D016328:NF-kappa B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D053592:Interleukin-1 Receptor-Associated Kinases; C507769:Irak1 protein, mouse
BCG; IRAK-1; Macrophage; Mycobacterium; microRNA