A gold nanoparticles enhanced surface plasmon resonance immunosensor for highly sensitive detection of ischemia-modified albumin.
In this study a novel sensitive nanogold particle sensor enhancement based on mixed self-assembled monolayers was explored and used to construct a Surface Plasmon Resonance (SPR) immunosensor to detect Ischemia Modified Albumin (IMA). Compared with a direct binding SPR assay at a limit of detection (LOD) of 100 ng/L, gold nanoparticles (AuNPs) of 10 nm dramatically improved the LOD of IMA to 10 ng/L. Meanwhile, no interfering substance that may lead to false positive results was identified. These results suggested that the SPR biosensor presented superior properties, and provided a simple label-free strategy to increase assay sensitivity for further acute coronary syndrome (ACS) diagnosis.
Laboratory of Biomedical Engineering, Chongqing Medical University, 1 Yixueyuan Road, Chongqing 400016, China. lcchen@cqmu.edu.cn.
Guang
G
Li
Xian
X
Li
Meng
M
Yang
Meng-Meng
MM
Chen
Long-Cong
LC
Chen
Xing-Liang
XL
Xiong
D015415:Biomarkers / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D017202:Myocardial Ischemia / Q000097:blood* / Q000175:diagnosis*; D053758:Nanoparticles; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012709:Serum Albumin; D000075462:Serum Albumin, Human; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
D015415:Biomarkers; D012709:Serum Albumin; C561522:ischemia-modified albumin; D006046:Gold; D000075462:Serum Albumin, Human