A novel organic-inorganic hybrid monolithic column prepared in-situ in a microchip and its application for the determination of 2-amino-4-chlorophenol in chlorzoxazone tablets.
γ-Alumina nanoparticles (γ-Al2O3) were introduced to the conventional poly(methacrylic acid-co-ethylene glycol dimethacrylate) (MAA-co-EGDMA) monolith to prepare a novel organic-inorganic hybrid monolith, poly(MAA-co-EGDMA)-Al2O3 monolith. The polymerization was induced in-situ with UV irradiation in an ultraviolet transparent polymethyl methacrylate (PMMA) microfluidic chip. The monolith-based solid phase microextraction system was used for the on-line determination of 2-amino-4-chlorophenol (ACP) in chlorzoxazone tablets coupled with an optical fiber spectrophotometer. Several parameters affecting the adsorption/desorption, including sample pH value, sample flow rate, sampling time, eluent flow rate, and eluting time, were investigated in detail. Under the optimized conditions, limit of detection (LOD) and limit of quantification (LOQ) of the method were calculated to be 2.8 and 9.1 µg L(-1), respectively, with the relative standard deviation (RSD) of 3.1%.
College of Chemistry, Jilin University, Qianjin Street 2699#, Changchun, 130012, PR China.
Junling
J
Zhang
Gang
G
Chen
Miaomiao
M
Tian
Ruigang
R
Li
Xinjun
X
Quan
Qiong
Q
Jia
D000327:Adsorption; D000537:Aluminum Oxide / Q000737:chemistry; D002733:Chlorophenols / Q000302:isolation & purification*; D002753:Chlorzoxazone / Q000032:analysis*; D004340:Drug Contamination; D005026:Ethylene Glycols / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008689:Methacrylates / Q000737:chemistry; D046210:Microfluidic Analytical Techniques; D009125:Muscle Relaxants, Central / Q000032:analysis*; D053758:Nanoparticles / Q000737:chemistry; D058105:Polymerization; D052617:Solid Phase Microextraction; D013607:Tablets / Q000737:chemistry*; D014466:Ultraviolet Rays
D002733:Chlorophenols; D005026:Ethylene Glycols; D008689:Methacrylates; D009125:Muscle Relaxants, Central; D013607:Tablets; C502776:poly(methacrylic acid-ethylene glycol dimethacrylate); C027296:2-amino-4-chlorophenol; D002753:Chlorzoxazone; D000537:Aluminum Oxide
2-amino-4-chlorophenol; Microfluidic chip; Optical fiber spectrophotometry; Organic–inorganic hybrid monolith; Solid phase microextraction