Transforming growth factor β1 genotypes in relation to TGFβ1, interleukin-8, and tumor necrosis factor alpha in induced sputum and blood in cystic fibrosis.
BACKGROUND
High-producer TGFβ1 genotypes are associated with severe lung disease in cystic fibrosis (CF), but studies combining IL-8, TNFα-, and TGFβ1(+genotype) levels and their impact on CF lung disease are scarce.


OBJECTIVE
Assessing the relationship between TGF β 1, IL-8, and TNF- α and lung disease in CF in an exacerbation-free interval.


METHODS
Twenty four patients delta F508 homozygous (median age 20.5 y, Shwachman score 75, FEV1(%) 83) and 8 controls (median age 27.5 y) were examined. TGF β 1 was assessed in serum and induced sputum (IS) by ELISA, for IL-8 and TNF- α by chemiluminescence in IS and whole blood. Genotyping was performed for TGF β 1 C-509T and T+869C utilizing RFLP.


RESULTS
TGF β 1 in IS (CF/controls median 76.5/59.1 pg/mL, P < 0.074) was higher in CF. There was a negative correlation between TGF β 1 in serum and lung function (LF) (FEV1 (r = -0.488, P = 0.025), MEF 25 (r = -0.425, P = 0.055), and VC (r = -0.572, P = 0.007)). Genotypes had no impact on TGF β 1 in IS, serum, and LF. In IS TGF β 1 correlated with IL-8 (r = 0.593, P < 0.007) and TNF- α (r = 0.536, P < 0.018) in patients colonized by bacteria with flagellin.


CONCLUSIONS
TGF β 1 in serum not in IS correlates with LF. In patients colonized by bacteria with flagellin, TGF β 1 correlates with IL-8 and TNF- α in IS.
Department of Pediatric Pulmonary, Goethe University Hospital, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany.
O
O
Eickmeier
L v D
Lv
Boom
F
F
Schreiner
M J
MJ
Lentze
D
D
NGampolo
R
R
Schubert
S
S
Zielen
S
S
Schmitt-Grohé
D000293:Adolescent; D000328:Adult; D001419:Bacteria; D002454:Cell Differentiation; D002648:Child; D003550:Cystic Fibrosis / Q000097:blood / Q000235:genetics* / Q000382:microbiology; D018572:Disease-Free Survival; D005260:Female; D005408:Flagellin; D005786:Gene Expression Regulation; D005838:Genotype; D006720:Homozygote; D006801:Humans; D016209:Interleukin-8 / Q000097:blood*; D008297:Male; D013147:Spirometry; D013183:Sputum / Q000737:chemistry*; D053773:Transforming Growth Factor beta1 / Q000097:blood / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*; D055815:Young Adult
D016209:Interleukin-8; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D005408:Flagellin