Characterization of fluoxetine effects on ethanol withdrawal-induced cortical hyperexcitability by EEG spectral power in rats.
Antidepressants have been used for treatment of ethanol withdrawal symptoms mainly in observational studies. However, cortical frequency oscillation of ethanol withdrawal treatment with antidepressants remained to be elucidated. This study aimed to identify surrogate biomarkers that represent intact biological or ethanol withdrawal processes and response to pretreatment with fluoxetine, a selective serotonin reuptake inhibitor, with quantitative methods. Adult male Wistar rats implanted with electrodes over the frontal and parietal cortices were rendered dependent on ethanol via modified liquid diet (MLD) containing ethanol. Then, ethanol-containing MLD was replaced with isocaloric ethanol-free MLD to induce ethanol withdrawal symptoms. Locomotor activity, sleep-wakefulness and spectral frequencies of electroencephalographic (EEG) pattern during ethanol-withdrawal period were analyzed. One-way ANOVA confirmed significant increases in locomotor activity and time spent in awake state and decreases time spent in non-rapid eye movement (NREM) sleep and REM-sleep during ethanol withdrawal period. Fast Fourier Transformation also revealed predominant increases in gamma spectral powers within both the frontal and parietal cortices during ethanol withdrawal. However, these changes, except sleep-wake disturbances, were significantly attenuated by fluoxetine pretreatment (10 mg/kg). The present study supports the hypothesis that serotonergic hypofunction may underlie most of ethanol withdrawal symptoms and proposes that electroencephalographic patterns are valid biomarkers for ethanol withdrawal evaluation and treatment.
Department of Physiology, Faculty of Science, Prince of Songkla University (PSU), Hat-Yai, Songkhla 90112, Thailand.
Dania
D
Cheaha
Department of Pharmacology, Faculty of Science, Prince of Songkla University (PSU), Hat-Yai, Songkhla 90112, Thailand.
Kitja
K
Sawangjaroen
Department of Physiology, Faculty of Science, Prince of Songkla University (PSU), Hat-Yai, Songkhla 90112, Thailand. Electronic address: ekumarnsit@yahoo.com.
Ekkasit
E
Kumarnsit
D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects* / Q000503:physiopathology; D004569:Electroencephalography; D000431:Ethanol / Q000009:adverse effects*; D005473:Fluoxetine / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017367:Selective Serotonin Reuptake Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D012890:Sleep / Q000187:drug effects; D013375:Substance Withdrawal Syndrome / Q000188:drug therapy / Q000503:physiopathology*; D014851:Wakefulness / Q000187:drug effects
D000431:Ethanol; D005473:Fluoxetine; D017367:Selective Serotonin Reuptake Inhibitors
EEG; Ethanol withdrawal; Fluoxetine; Gamma oscillation; Serotonin