Functional genomics in the study of yeast cell polarity: moving in the right direction.
The budding yeast Saccharomyces cerevisiae has been used extensively for the study of cell polarity, owing to both its experimental tractability and the high conservation of cell polarity and other basic biological processes among eukaryotes. The budding yeast has also served as a pioneer model organism for virtually all genome-scale approaches, including functional genomics, which aims to define gene function and biological pathways systematically through the analysis of high-throughput experimental data. Here, we outline the contributions of functional genomics and high-throughput methodologies to the study of cell polarity in the budding yeast. We integrate data from published genetic screens that use a variety of functional genomics approaches to query different aspects of polarity. Our integrated dataset is enriched for polarity processes, as well as some processes that are not intrinsically linked to cell polarity, and may provide new areas for future study.
The Donnelly Centre, University of Toronto, , 160 College St., Toronto, Ontario, Canada , M5S 3E1.
Erin
E
Styles
Ji-Young
JY
Youn
Mojca
M
Mattiazzi Usaj
Brenda
B
Andrews
D016764:Cell Polarity / Q000502:physiology*; D030541:Databases, Genetic; D023281:Genomics / Q000379:methods* / Q000639:trends; D057166:High-Throughput Screening Assays / Q000379:methods* / Q000639:trends; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology*; D020846:cdc42 GTP-Binding Protein, Saccharomyces cerevisiae / Q000378:metabolism
D020846:cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
Saccharomyces cerevisiae; functional genomics; genetics; microscopy; polarity; proteomics