[Analysis of allele dropout at TH01 locus in paternity testing].
OBJECTIVE
To analyze allele dropout at TH01 locus in paternity testing in order to determine the accurate genotype.


METHODS
To use a two STR loci genotyping system to verify an abnormal genotype for the TH01 locus with PCR using specific primers, cloning and DNA sequencing.


RESULTS
A rare allele at TH01 locus named 5.2, which was undetectable with PowerPlex 21 system, was detected with an Identifiler system.


CONCLUSIONS
Genetic variations may result in rare alleles and loci loss. To avoid misjudgment, laboratories should have a variety of methods for detecting loci loss.
Forensic Science Institute of Fujian Provincial Hospital, Fujian Provincial Key Laboratory of Cardiovascular Disease, Provincial Clinical College of Fujian Medical University, Fuzhou, Fujian 350001, P. R. China. Email: sxli3318@163.com.
Li
L
Lai
Xiao-li
XL
Shen
Shi-jie
SJ
Xue
Jie
J
Hu
D000328:Adult; D000483:Alleles; D001483:Base Sequence; D002675:Child, Preschool; D016172:DNA Fingerprinting; D005820:Genetic Testing; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010334:Paternity; D016133:Polymerase Chain Reaction; D017384:Sequence Deletion; D020080:Tandem Repeat Sequences