Highly sensitive and selective rhodamine-based "off-on" reversible chemosensor for tin (Sn4+) and imaging in living cells.
A structurally characterized new oxo-chromene functionalized rhodamine derivative L1 exhibits high selectivity toward Sn(4+) by forming a 1:1 complex, among other biologically important metal ions, as studied by fluorescence, absorption, and HRMS spectroscopy. Complexing with Sn(4+) triggers the formation of a highly fluorescent ring-open form which is pink in color. The sensor shows extremely high fluorescence enhancement upon complexation with Sn(4+), and it can be used as a "naked-eye" sensor. DFT computational studies carried out in mimicking the formation of a 1:1 complex between L1 and Sn(4+) resulted in a nearly planar pentacoordinate Sn(IV) complex. Studies reveal that the in situ prepared L1-Sn complex is selectively and fully reversible in presence of sulfide anions. Further, confocal microscopic studies confirmed that the receptor shows in vitro detection of Sn(4+) ions in RAW cells.
Department of Chemistry and ‡Centre for Healthcare Science & Technology, Bengal Engineering and Science University , Shibpur, Howrah-711103, India.
Ajit Kumar
AK
Mahapatra
Saikat Kumar
SK
Manna
Debasish
D
Mandal
Chitrangada
C
Das Mukhopadhyay
D045744:Cell Line, Tumor; D056831:Coordination Complexes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D019044:Image Cytometry; D057230:Limit of Detection; D018613:Microscopy, Confocal; D015394:Molecular Structure; D011789:Quantum Theory; D012235:Rhodamines / Q000737:chemistry* / Q000145:classification; D014001:Tin / Q000032:analysis / Q000737:chemistry*
D056831:Coordination Complexes; D005456:Fluorescent Dyes; D012235:Rhodamines; D014001:Tin