Enantioselective degradation of metalaxyl in cucumber, cabbage, spinach and pakchoi.
The enantioselective degradation behaviors of the acylamino acid fungicide metalaxyl in vegetables (cucumber, cabbage, spinach and pakchoi) under field and greenhouse conditions were elucidated by reversed-phase high-performance liquid chromatography (HPLC). A cellulose tris (3-chloro-4-methyl phenyl carbamate) chiral column (Lux Cellulose-2) was used for the chiral separation of the enantiomers using acetonitrile-0.1% formic acid solution (40:60, v/v) as mobile phase at a flow rate of 1.0 mL min(-1). The two enantiomers could be completely separated with a resolution (Rs) of 5.2. The linearity range, matrix effect, precision and recovery were evaluated. The method was successfully applied for the enantioselective analysis of metalaxyl in cucumber, cabbage, spinach and pakchoi under different conditions. The results showed that R-enantiomer dissipated faster than S-enantiomer in cucumber of greenhouse. Inversely, a preferential degradation of S-form was found in spinach and pakchoi under field and greenhouse conditions. No stereoselective degradation in cabbage was observed. These results can be applied for evaluating the environmental risk and food safety.
Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Jiangsu Key Laboratory of Pesticide Science, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, PR China.
Meiyun
M
Wang
Qing
Q
Zhang
Lujing
L
Cong
Wei
W
Yin
Minghua
M
Wang
D000409:Alanine / Q000031:analogs & derivatives* / Q000378:metabolism; D001937:Brassica / Q000378:metabolism*; D018553:Cucumis sativus / Q000378:metabolism*; D005659:Fungicides, Industrial / Q000378:metabolism*; D012989:Soil Pollutants / Q000378:metabolism; D018724:Spinacia oleracea / Q000378:metabolism*; D013237:Stereoisomerism
D005659:Fungicides, Industrial; D012989:Soil Pollutants; C028175:metalaxyl; D000409:Alanine
Cabbage; Cucumber; Enantioselective degradation; Metalaxyl; Pakchoi; Spinach