Structure of fructo-oligosaccharides from leaves and stem of Agave tequilana Weber, var. azul.
Fructo-oligosaccharides (FOSs) of a six year old agave plant variety, Agave tequilana, were isolated and fractionated by 2D preparative chromatography (SEC and rpHPLC). Structural analyses of different FOS-fractions were performed by reductive methylation analysis connected to GC/FID identification and NMR-analysis. FOSs from leaves (d.p. 3-8) contain single α-d-Glcp residues as well in terminal as internal position, however (2→1)-linked β-d-Fruf residues only. FOSs from stem, however, contain as well (2→1)- and (2→6)-linked β-d-Fruf residues with branched oligomeric repeating units. These characteristics indicate an enzymatically catalyzed metabolic regulation for the biosynthesis and transformation of fructans in A. tequilana which strongly depends on location and transport activities.
Department of Chemistry, BOKU, University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria; Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstr. 14, 1090 Vienna, Austria. Electronic address: werner.praznik@univie.ac.at.
Werner
W
Praznik
Renate
R
Löppert
Josè M
JM
Cruz Rubio
Klaus
K
Zangger
Anton
A
Huber
D027882:Agave / Q000737:chemistry*; D002236:Carbohydrate Conformation; D005632:Fructose / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry*
D009844:Oligosaccharides; D005632:Fructose
2D-liquid chromatography; Agave tequilana; Fructo-oligosaccharides; Metabolic regulation; Structure analysis