Biotinidase knockout mice show cellular energy deficit and altered carbon metabolism gene expression similar to that of nutritional biotin deprivation: clues for the pathogenesis in the human inherited disorder.
Biotin is the prosthetic group of carboxylases that have important roles in the metabolism of glucose, fatty acids and amino acids. Biotinidase has a key role in the reutilization of the biotin, catalyzing the hydrolysis of biocytin (ε-N-biotinyl-l-lysine) and biocytin-containing peptides derived from carboxylase turnover, thus contributing substantially to the bioavailability of this vitamin. Deficient activity of biotinidase causes late-onset multiple carboxylase in humans, whose pathogenic mechanisms are poorly understood. Here we show that a knock-out biotinidase-deficient mouse from a C57BL/6 background that was fed a low biotin diet develops severe ATP deficit with activation of the energy sensor adenosine monophosphate (AMP)-activated protein kinase (AMPK), inhibition of the signaling protein mTOR, driver of protein synthesis and growth, and affecting the expression of central-carbon metabolism genes. In addition, sensitivity to insulin is augmented. These changes are similar to those observed in nutritionally biotin-starved rats. These findings further our understanding of the pathogenesis of human biotinidase deficiency.
Unidad de Genética de la Nutrición, Instituto de Investigaciones Biomédicas-Universidad Nacional Autónoma de México-Instituto Nacional de Pediatría, México City, México.
A
A
Hernández-Vázquez
B
B
Wolf
K
K
Pindolia
D
D
Ortega-Cuellar
R
R
Hernández-González
A
A
Heredia-Antúnez
I
I
Ibarra-González
A
A
Velázquez-Arellano
D000818:Animals; D001710:Biotin / Q000172:deficiency / Q000378:metabolism; D028921:Biotinidase Deficiency / Q000178:diet therapy / Q000235:genetics* / Q000378:metabolism*; D001786:Blood Glucose; D001835:Body Weight; D002244:Carbon / Q000378:metabolism; D002331:Carnitine / Q000031:analogs & derivatives / Q000378:metabolism; D004032:Diet; D004195:Disease Models, Animal; D004734:Energy Metabolism / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D008099:Liver / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D001786:Blood Glucose; D012333:RNA, Messenger; C116917:acylcarnitine; D001710:Biotin; D002244:Carbon; D002331:Carnitine
AMPK; Biotin deficiency; Biotinidase knockout; Insulin sIgnaling pathway; Insulin sensitivity; energetic deficit