Protective effects of alkaloid compounds from Nelumbinis Plumula on tert-butyl hydroperoxide-induced oxidative stress.
This study was conducted to investigate the effect of Nelumbinis Plumula total alkaloid (NPA) and its main alkaloid components on oxidative stress induced by tert-butyl hydroperoxide (t-BHP) in the human hepatocellular HepG2 cell line. According to HPLC analysis, several major alkaloid compounds such as liensinine, isoliensinine and neferine were present in NPA. The cytotoxic effects in 0.55 mM t-BHP-induced HepG2 cells were significantly inhibited by NPA and the major compound in NPA, neferine, showed the strongest activities. The protective effect of neferine against oxidative stress induced by t-BHP may be associated with decreased ROS formation, TBARS generation, LDH release and increased GSH levels, suggesting their involvement of the cytoprotective on oxidative stress. The effects were comparable with quercetin, which was used as positive control. Overall, total alkaloid and alkaloid compounds from Nelumbinis Plumula displayed a significant cytoprotective effect against oxidative stress. Further study is needed to elucidate the relationship between the chemical structures of the components in NPA and their protective effect on oxidative stress.
College of Food Science and technology, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China. zbdfst@163.com.
Yong
Y
Xie
Yi
Y
Zhang
Long-Tao
LT
Zhang
Shao-Xiao
SX
Zeng
Ze-Bin
ZB
Guo
Bao-Dong
BD
Zheng
D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology; D044182:Benzylisoquinolines / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D004353:Drug Evaluation, Preclinical; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D005609:Free Radicals / Q000737:chemistry; D005978:Glutathione; D056945:Hep G2 Cells; D006801:Humans; D007546:Isoquinolines / Q000737:chemistry / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D031653:Nelumbo / Q000737:chemistry*; D016877:Oxidants / Q000633:toxicity; D018384:Oxidative Stress; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D020122:tert-Butylhydroperoxide / Q000633:toxicity
D000470:Alkaloids; D044182:Benzylisoquinolines; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D007546:Isoquinolines; D016877:Oxidants; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; C499779:isoliensinine; C057222:neferine; C080095:liensinine; D008315:Malondialdehyde; D020122:tert-Butylhydroperoxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D007770:L-Lactate Dehydrogenase; D005978:Glutathione