Treatment of ischemic colonic anastomoses with systemic transplanted bone marrow derived mesenchymal stem cells.
BACKGROUND
The aim of the study is to investigate the healing effect of the bone-marrow derived mesenchymal stem cells (BM-MSCs) on ischemic colon anastomosis in systemic application and to recovery the adverse effect of ischemia.


METHODS
Fourty male Wistar Albino rats weigthing 250-300 g were divided into four equal groups (n=10 Group 1: control; ischemic left colonic anastomoses (4th day); Group 2: control; ischemic left colonic anastomoses (7th day); Group 3: ischemic left colonic anastomoses + systemic transplanted BM-MSCs (4th day); Group 4: ischemic left colonic anastomoses + systemic transplanted BM-MSCs (7th day). BMSCs labelled with bromodeoxyuridine (BrdU) were transplanted into the vena cava. Group 1 and group 3 were killed four days after surgery. In group 2 and group 4 were sacrificed seven days after the surgical procedure. Histopathological features, hydroxyproline levels in the tissue, and anastomotic strength were investigated.


RESULTS
There was no mortality all of the groups.The mean bursting pressures of ischemic colonic anastomoses in group 3 were higher than in control group 1 (4th day). We found significantly higher hydroxyproline values in group 3 and were significantly higher in group 4 than in control groups. We investigated the early period of wound healing (4th day and 7th day). When comparing between group 1 and group 3, we found higher levels for all of the histological parameters except inflammation in group 3. On day 7, when comparing between group 2 and group 4, we found higher levels for parameters of necrosis, collagen deposition.


CONCLUSIONS
BM-MSCs therapy significantly accelerated all of the healing parameters for ischemic colonic anastomosis except for inflammation on fourth day. On the seventh day, BM-MSCs augmented the levels of the hydroxyproline. Histological parameters, necrosis and collagen deposition were also found to be important for healing of ischemic colonic anastomoses. However, they did not accelerate the others histological parameters especially angiogenesis.
Department of General Surgery, Bakirkoy Sadi Konuk Training and Research Hospital, Istanbul, Turkey. ozgurkemik@hotmail.com  
G
G
Adas
O
O
Kemik
B
B
Eryasar
A
A
Okcu
M
M
Adas
S
S
Arikan
G
G
Erman
A S
AS
Kemik
G
G
Kamali
Y
Y
Dogan
E
E
Karaoz
D000714:Anastomosis, Surgical; D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods; D001973:Bromodeoxyuridine / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D003106:Colon / Q000098:blood supply / Q000473:pathology / Q000601:surgery*; D007511:Ischemia / Q000473:pathology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D009336:Necrosis / Q000209:etiology; D018919:Neovascularization, Physiologic / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014945:Wound Healing
D003094:Collagen; D001973:Bromodeoxyuridine