Point-of-care nucleic acid detection using nanotechnology.
Recent developments in nanotechnology have led to significant advancements in point-of-care (POC) nucleic acid detection. The ability to sense DNA and RNA in a portable format leads to important applications for a range of settings, from on-site detection in the field to bedside diagnostics, in both developing and developed countries. We review recent innovations in three key process components for nucleic acid detection: sample preparation, target amplification, and read-out modalities. We discuss how the advancements realized by nanotechnology are making POC nucleic acid detection increasingly applicable for decentralized and accessible testing, in particular for the developing world.
Department of Biological and Environmental Engineering, Cornell University, Ithaca, New York, USA. dan.luo@cornell.edu.
Mark R
MR
Hartman
Roanna C H
RC
Ruiz
Shogo
S
Hamada
Chuanying
C
Xu
Kenneth G
KG
Yancey
Yan
Y
Yu
Wei
W
Han
Dan
D
Luo
D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis*; D055664:Electrochemical Techniques; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology / Q000295:instrumentation; D021141:Nucleic Acid Amplification Techniques; D019095:Point-of-Care Systems; D012313:RNA / Q000032:analysis*
D012313:RNA; D004247:DNA