A multiparameter live cell imaging approach to monitor cyclic AMP and protein kinase A dynamics in parallel.
Parallel detection of signaling activities allows us to correlate activity dynamics between signaling molecules. In this review, we detail a multiparameter live cell imaging method to monitor 3',5'-cyclic adenosine monophosphate (cAMP) levels and protein kinase A (PKA) activities in parallel.
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Nwe-Nwe
NN
Aye-Han
Jin
J
Zhang
D002470:Cell Survival; D000242:Cyclic AMP / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted; D057054:Molecular Imaging / Q000379:methods*; D014162:Transfection
D005456:Fluorescent Dyes; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases