Elevated serum ubiquitin C-terminal hydrolase-L1 levels in patients with carbon monoxide poisoning.
OBJECTIVE
Ubiquitin C-terminal hydrolase-L1 (UCH-L1) has been established as a reliable and potential biomarker of neuronal damage after acute neurologic insults, such as ischemic stroke, subarachnoid hemorrhage, and traumatic brain injury. However, the effect of serum UCH-L1 levels has not been investigated in carbon monoxide (CO)-poisoned patients. The aim of the present study was to evaluate whether serum UCH-L1 levels are a reliable marker of brain damage and the association of UCH-L1 with outcome.


METHODS
This case-control study enrolled 46 CO-poisoned subjects and 30 controls. Using an enzyme-linked immunosorbent assay (ELISA) kit, we studied the temporal profile of serum UCH-L1 levels at 6, 12, 24 and 48 h after acute CO poisoning. Poisoning severity was assessed using the Glasgow Coma Scale (GCS) score. Long-term outcome was assessed using the Glasgow Outcome Scale (GOS) at 6 months after poisoning.


RESULTS
Compared with controls, CO-poisoned patients had significantly elevated serum levels of UCH-L1 at each time point after poisoning. There were significantly higher levels of UCH-L1 in CO-poisoned patients with a lower GCS score as well as in those with a poor 6-month outcome dichotomized GOS.


CONCLUSIONS
Serum levels of UCH-L1 appear to have potential clinical utility in providing valuable information about poisoning severity and outcome after CO poisoning.
Department of Emergency, The First Affiliated Hospital of Jilin University, Changchun, Jilin, PR China.
Li
L
Pang
Department of Emergency, The First Affiliated Hospital of Jilin University, Changchun, Jilin, PR China.
Yang
Y
Wu
Department of Emergency, The First Affiliated Hospital of Jilin University, Changchun, Jilin, PR China.
Ning
N
Dong
Department of Emergency, The First Affiliated Hospital of Jilin University, Changchun, Jilin, PR China.
Da-hai
DH
Xu
Department of Emergency, The First Affiliated Hospital of Jilin University, Changchun, Jilin, PR China.
Da-wei
DW
Wang
Department of Emergency, The First Affiliated Hospital of Jilin University, Changchun, Jilin, PR China.
Zhi-hao
ZH
Wang
Department of Emergency, The First Affiliated Hospital of Jilin University, Changchun, Jilin, PR China.
Xing-liang
XL
Li
Department of Respiratory, Qianwei Hospital of Jilin Province, Changchun, Jilin, PR China.
Miao
M
Bian
Department of Respiratory, Qianwei Hospital of Jilin Province, Changchun, Jilin, PR China.
Hui-jie
HJ
Zhao
Department of Emergency, The First Affiliated Hospital of Jilin University, Changchun, Jilin, PR China.
Xiao-liang
XL
Liu
Department of Emergency, The First Affiliated Hospital of Jilin University, Changchun, Jilin, PR China. Electronic address: 466634002@qq.com.
Nan
N
Zhang
D000328:Adult; D002249:Carbon Monoxide Poisoning / Q000097:blood / Q000201:enzymology*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D043222:Ubiquitin Thiolesterase / Q000097:blood*; D055815:Young Adult
C482860:UCHL1 protein, human; D043222:Ubiquitin Thiolesterase
Biomarkers; Brain damage; CO; CO poisoning; COHb; ED; ELISA; GCS; GOS; Glasgow Coma Scale; Glasgow Outcome Scale; HBO; HBO therapy; HBOT; NBO; SEM; TBI; UCH-L1; Ubiquitin C-terminal hydrolase L1; carbon monoxide; carboxyhemoglobin; emergency department; enzyme-linked immunosorbent assay; hyperbaric oxygen; normobaric oxygen; standard error of the mean; traumatic brain injury; ubiquitin C-terminal hydrolase-L1