Chromosome 7 aneuploidy in clear cell and papillary renal cell carcinoma: detection using silver in situ hybridization technique.
BACKGROUND
Chromosome 7 aberrations in renal cell carcinoma (RCC) have been reported in papillary renal cell carcinoma (pRCC) and clear cell renal cell carcinoma (ccRCC). However, the implication of these anomalies on prognosis and survival is still unclear. RCC Chromosome 7 aberrations have commonly been detected by fluorescent in situ hybridization and chromogenic in situ hybridization but not silver in situ hybridization (SISH).


OBJECTIVE
The purpose was to report chromosome 7 aberrations in ccRCC and pRCC using SISH in paraffin-embedded tissues and determine the association between the anomalies with clinical and pathological features.


METHODS
Cases of ccRCC and pRCC from University Malaya Medical Centre (2001-2009) were analyzed. Chromosome 7 staining was performed using an automated SISH method and association tests between chromosomal anomalies, clinical features and survival were performed.


RESULTS
SISH is a feasible technique to detect chromosome 7 aberration in RCC. Chromosome 7 aberrations with nuclear grading, staging and survival yielded no significant correlation. Surprisingly, there was a significant association between gender and chromosome 7 expressions. Though grade did not reach statistical significance for survival in our RCC cases, there was a significant correlation between overall survival with race and stage.


CONCLUSIONS
Chromosome 7 aberrations in ccRCC showed no prognostic significance. Nevertheless, staging and grading systems that include prognostic variables could hold better promise.
Department of Surgery, Faculty of Medicine, University of Malaya Cancer Research Institute, University of Malaya, Malaysia; Centre for Kidney Disease Research, School of Medicine, University of Queensland, Australia, .
Jayalakshmi
J
Pailoor
Retnagowri
R
Rajandram
Ning Yi
NY
Yap
Keng Lim
KL
Ng
Zhiqiang
Z
Wang
Krishnan R
KR
Iyengar
D000046:Academic Medical Centers; D000328:Adult; D000368:Aged; D000782:Aneuploidy; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D002897:Chromosomes, Human, Pair 7; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D008296:Malaysia; D008297:Male; D008875:Middle Aged; D012720:Severity of Illness Index; D016019:Survival Analysis