[Gene expressions of LOXs and MMPs of the ACL fibroblasts cells co-cultured with synovial cells].
The progress of research on the the anterior cruciate ligament (ACL) wound healing demonstrates that the synovial tissue in the knee joint plays a very important role in the healing process of injured ACL. Therefore, the molecular response mechanisms of lysyl oxidase (LOX) and matrix metalloproteina (MMP) in normal/injured ACL fibroblast cells could be considered to perform the major analysis function of injured ACL healing mechanism. The mRNA expressions of LOXs and MMPs and the activity expressions of MMP-2 in ACL fibroblasts co-cultured with synovial cells were analyzed by quantitative real-time PCR and zymography. The results showed that co-culture could regulate the mRNA expressions of LOXs and MMPs in the ACL fibroblasts cells. These results suggest that the differential expressions of LOXs and MMP-1, 2, 3 in co-cultured ACL indicate that interaction crosstalk do exist between ACL cells and synovial cells and provide a theoretical basis for subsequent exploration of the mechanisms and treatment of ACL injury and repair.
College of Bioengineering, Chongqing University, Chongqing 400044, China.
Chunli
C
Wang
Hu
H
Mei
Jing
J
Xie
Jiahuan
J
Jiang
Rongfu
R
Chen
Lin
L
Yin
Chunfeng
C
Fu
Cheng
C
Chen
K L Paul
KL
Sung
D016118:Anterior Cruciate Ligament / Q000166:cytology*; D000070598:Anterior Cruciate Ligament Injuries; D018920:Coculture Techniques; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007718:Knee Injuries / Q000503:physiopathology; D007719:Knee Joint / Q000166:cytology; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism*; D008249:Protein-Lysine 6-Oxidase / Q000235:genetics / Q000378:metabolism*; D013583:Synovial Membrane / Q000166:cytology*; D014945:Wound Healing / Q000502:physiology
D008249:Protein-Lysine 6-Oxidase; D020782:Matrix Metalloproteinases