The luminal progenitor compartment of the normal human mammary gland constitutes a unique site of telomere dysfunction.
Telomeres are essential for genomic integrity, but little is known about their regulation in the normal human mammary gland. We now demonstrate that a phenotypically defined cell population enriched in luminal progenitors (LPs) is characterized by unusually short telomeres independently of donor age. Furthermore, we find that multiple DNA damage response proteins colocalize with telomeres in >95% of LPs but in <5% of basal cells. Paradoxically, 25% of LPs are still capable of exhibiting robust clonogenic activity in vitro. This may be partially explained by the elevated telomerase activity that was also seen only in LPs. Interestingly, this potential telomere salvage mechanism declines with age. Our findings thus reveal marked differences in the telomere biology of different subsets of primitive normal human mammary cells. The chronically dysfunctional telomeres unique to LPs have potentially important implications for normal mammary tissue homeostasis as well as the development of certain breast cancers.
Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC V5Z 1L3, Canada.
Nagarajan
N
Kannan
Nazmul
N
Huda
Liren
L
Tu
Radina
R
Droumeva
Geraldine
G
Aubert
Elizabeth
E
Chavez
Ryan R
RR
Brinkman
Peter
P
Lansdorp
Joanne
J
Emerman
Satoshi
S
Abe
Connie
C
Eaves
David
D
Gilley
D000293:Adolescent; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D000368:Aged; D002478:Cells, Cultured; D004249:DNA Damage; D005260:Female; D006801:Humans; D042361:Mammary Glands, Human / Q000166:cytology* / Q000378:metabolism; D008875:Middle Aged; D019098:Telomerase / Q000235:genetics / Q000378:metabolism; D016615:Telomere / Q000235:genetics*; D059506:Telomere Shortening
C509186:TERT protein, human; D019098:Telomerase