A novel amide stationary phase for hydrophilic interaction liquid chromatography and ion chromatography.
A novel amide stationary phase (ASP) for hydrophilic interaction liquid chromatography (HILIC) has been prepared via the Click chemistry method. It was based on the strategy that the amino group of Asparagine was easily transferred to the corresponding azido group and then clicked onto terminal alkyne-silica gel in the presence of Cu(I)-based catalyst. For the tested polar compounds including nucleosides and nucleic acid bases, ASP-based column has demonstrated good performance in terms of separation efficiency and column stability, and the retention mechanism was found to match well the typical HILIC retention. In addition, the ASP described here showed much better selectivity in separation of inorganic anions under ion chromatography mode relative to other kinds of commercial ASP.
School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
Guobin
G
Shen
Feifang
F
Zhang
Bingcheng
B
Yang
Changhu
C
Chu
Xinmiao
X
Liang
D000480:Alkynes / Q000737:chemistry; D001216:Asparagine / Q000737:chemistry*; D002384:Catalysis; D002414:Cations, Monovalent; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D057930:Click Chemistry; D003300:Copper / Q000737:chemistry; D005782:Gels; D057927:Hydrophobic and Hydrophilic Interactions; D009705:Nucleosides / Q000302:isolation & purification*; D011685:Purine Nucleotides / Q000302:isolation & purification*; D011742:Pyrimidine Nucleotides / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry
D000480:Alkynes; D002414:Cations, Monovalent; D005782:Gels; D009705:Nucleosides; D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides; D001216:Asparagine; D012822:Silicon Dioxide; D003300:Copper
Amide stationary phase; Click chemistry; Hydrophilic interaction liquid chromatography; Ion chromatography