Gibberellic acid acts as an agonist of steroidogenesis in male rats.
Testicular steroidogenesis has significant implication in male reproductive function. Although the effects of various signalling molecules on testicular functions have been studied earlier, the influence of the plant hormone gibberellic acid (GA3 ) on steroidogenesis has not been investigated. Acute (4 h) and subacute (15 days) studies using this compound through oral administration (150 μg day(-1) ) to groups of normal and diabetic Wistar male rats were therefore carried out. Results indicate that (i) enhanced activity of steroidogenic markers 3β-hydroxysteroid dehydrogenase (3β-HSD), 17β-hydroxysteroid dehydrogenase (17β-HSD), elevated tissue testosterone (T) content, increased steroidogenic acute regulatory protein (StAR) and androgen binding protein (ABP) levels with reduced lipid peroxidation and improved antioxidant defence in this treatment group of normal and diabetic rat testis, and (ii) elevated lipid peroxidation and diminished antioxidant defence, with insignificant change in 3β-HSD and 17β-HSD activity and testosterone level in acute treatment group of normal and diabetic rats testis, were noted. The observed increase in the activity of testicular 3β-HSD and 17β-HSD along with elevated testosterone content established GA3 as an inducer of steroidogenesis in rat.
Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Pondicherry, India.
R
R
Premalatha
R
R
Jubendradass
K
K
Srikumar
P P
PP
Mathur
D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000727:Androgen-Binding Protein / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D005875:Gibberellins / Q000494:pharmacology*; D012739:Gonadal Steroid Hormones / Q000096:biosynthesis*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D010750:Phosphoproteins / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology*; D017208:Rats, Wistar; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D000098682:Steroidogenic Acute Regulatory Protein
D000727:Androgen-Binding Protein; D000975:Antioxidants; D005875:Gibberellins; D012739:Gonadal Steroid Hormones; D010750:Phosphoproteins; D010937:Plant Growth Regulators; D000098682:Steroidogenic Acute Regulatory Protein; C007842:gibberellic acid; D015067:17-Hydroxysteroid Dehydrogenases; D015096:3-Hydroxysteroid Dehydrogenases
Gibberellic acid; lipid peroxidation; phytohormone; rat testis; steroidogenesis