Co-stimulation with LPS or Poly I:C markedly enhances the anti-platelet immune response and severity of fetal and neonatal alloimmune thrombocytopenia.
Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is a life-threatening bleeding disorder caused by maternal antibodies against fetal/neonatal platelets. FNAIT is also linked with miscarriages, although the incidence and mechanisms of fetal death have not been well studied. IntegrinαIIbβ3 (GPIIbIIIa) and the GPIbα complex are major glycoproteins expressed on platelets and are also major antigens targeted in autoimmune thrombocytopenia (ITP), but reported cases of anti-GPIb-mediated FNAIT are rare. Bacterial and viral infections have been causally linked with the pathogenesis of immune-mediated thrombocytopenia (ITP); however, it is unknown whether these infections contribute to the severity of FNAIT. Here, immune responses against platelet antigens were examined by transfusing wild-type (WT) mouse platelets into β3-/- or GPIbα-/- mice. To mimic bacterial or viral infections, lipopolysaccharide (LPS) or polyinosinic:polycytidylic acid (Poly I:C) were injected intraperitoneally following platelet transfusions. The FNAIT model was established by breeding the immunised female mice with WT male mice. We demonstrated for the first time that the platelet GPIbα has lower immunogenicity compared to β3 integrin. Interestingly, co-stimulation with LPS or Poly I:C markedly enhanced the immune response against platelet GPIbα and caused severe pathology of FNAIT (i.e. miscarriages). LPS or Poly I:C also enhanced the immune response against platelet β3 integrin. Our data suggest that bacterial and viral infections facilitate the anti-platelet GPIbα response, which may lead to a severe non-classical FNAIT (i.e. miscarriage but not neonatal bleeding) that has not been adequately reported in humans.
Heyu Ni, MD, PhD, Canadian Blood Services and Department of Laboratory Medicine and Pathobiology, University of Toronto, St. Michael's Hospital, Room 420, LKSKI - Keenan Research Centre, 209 Victoria Street, Toronto, Ontario M5B 1W8, Canada, Tel.: +1 416 847 1738, E-mail: nih@smh.ca.
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Pingguo
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D000022:Abortion, Spontaneous / Q000209:etiology / Q000276:immunology*; D000818:Animals; D001324:Autoantigens / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology*; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D005315:Fetal Diseases / Q000276:immunology*; D006801:Humans; D007109:Immunity; D007114:Immunization; D007239:Infections / Q000150:complications / Q000276:immunology*; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000276:immunology; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D017713:Platelet Transfusion; D011070:Poly I-C / Q000008:administration & dosage; D011247:Pregnancy; D054098:Thrombocytopenia, Neonatal Alloimmune / Q000276:immunology*
D001324:Autoantigens; D008070:Lipopolysaccharides; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D019038:Platelet Glycoprotein GPIb-IX Complex; C094990:adhesion receptor; D011070:Poly I-C
Alloimmune thrombocytopenia; GPIbα; infection; platelets; β3 integrin