Nutrient limitation in Auxenochlorella protothecoides induces qualitative changes of fatty acid and expression of caleosin as a membrane protein associated with oil bodies.
Oil bodies formed in Auxenochlorella protothecoides induced during limited nutrition had a coating of caleosin. The total lipid content obtained from A. protothecoides in unstressed cultures (first week) was ~210 mg/g compared to the 231 mg/g obtained in the third week (nutrient limited) and 290 mg/g obtained in the fourth week (nutrient limited). The proportion of total saturated fatty acids increased from 28 to 46 %, whereas that of total polyunsaturated fatty acids decreased from 52 to 35 %. The expression levels of the 28 kDa caleosin protein in A. protothecoides rose to a maximum up to 4 weeks; immunolocalization studies showed that caleosin was predominantly associated with the membranes of oil bodies.
Buntora
B
Pasaribu
I-Ping
IP
Lin
Chii-Shiarng
CS
Chen
Chi-Yu
CY
Lu
Pei-Luen
PL
Jiang
D002135:Calcium-Binding Proteins / Q000032:analysis / Q000378:metabolism*; D000460:Chlorophyta / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D015388:Organelles / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D005227:Fatty Acids; D008565:Membrane Proteins; D010940:Plant Proteins; C402836:caleosin protein, Sesamum indicum; D009584:Nitrogen