Production of ginsenosides Rg1 and Rh1 by hydrolyzing the outer glycoside at the C-6 position in protopanaxatriol-type ginsenosides using β-glucosidase from Pyrococcus furiosus.
The specific activity of a recombinant β-glucosidase from Pyrococcus furiosus for protopanaxatriol (PPT)-type ginsenosides followed the order Rf > R1 > Re > R2 > Rg2, which were converted to Rh1, Rg1, Rg1, Rh1, and Rh1, respectively. No activity was observed with Rg1 and Rh1. Thus, P. furiosus β-glucosidase hydrolyzed the outer glycoside at the C-6 position in PPT-type ginsenosides whereas the enzyme did not hydrolyze the inner glucoside at the C-6 position and the glucoside at the C-20 position. The activity for Rf was optimal at 95 °C, pH 5.5, 5 mM ginsenoside, and 32 U enzyme l−1. Under these conditions, P. furiosus β-glucosidase completely converted from R1 to Rg1 after 10 h, with a productivity of 0.4 g l−1 h−1 and completely converted Rf to Rh1 after 1.2 h, with a productivity of 2.74 g l−1 h−1.
Hye-Jin
HJ
Oh
Kyung-Chul
KC
Shin
Deok-Kun
DK
Oh
D019843:Archaeal Proteins / Q000378:metabolism*; D001711:Biotransformation; D036145:Ginsenosides / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D006358:Hot Temperature; D020027:Pyrococcus furiosus / Q000201:enzymology* / Q000235:genetics; D012502:Sapogenins / Q000737:chemistry / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism*
D019843:Archaeal Proteins; D036145:Ginsenosides; D012502:Sapogenins; C081552:protopanaxatriol; D001617:beta-Glucosidase; C035054:ginsenoside Rg1