Continuous production of β-cyclodextrin from starch by highly stable cyclodextrin glycosyltransferase immobilized on chitosan.
Cyclodextrin glycosyltransferase (CGTase) from Thermoanaerobacter sp. was covalently immobilized on glutaraldehyde-activated chitosan spheres and used in a packed bed reactor to investigate the continuous production of β-cyclodextrin (β-CD). The optimum temperatures were 75 °C and 85 °C at pH 6.0, respectively for free and immobilized CGTase, and the optimum pH (5.0) was the same for both at 60 °C. In the reactor, the effects of flow rate and substrate concentration in the β-CD production were evaluated. The optimum substrate concentration was 4% (w/v), maximizing the β-CD production (1.32 g/L) in a flow rate of 3 mL/min. In addition, the biocatalyst had good operational stability at 60 °C, maintaining 61% of its initial activity after 100 cycles of batch and 100% after 100 h of continuous use. These results suggest the possibility of using this immobilized biocatalyst in continuous production of CDs.
Biotechnology, Bioprocess and Biocatalysis Group, Institute of Food Science and Technology, Federal University of Rio Grande do Sul, Avenida Bento Gonçalves, 9500, P.O. Box 15090, ZC 91501-970, Porto Alegre, RS, Brazil.
Jéssie da Natividade
Jda N
Schöffer
Manuela Poletto
MP
Klein
Rafael C
RC
Rodrigues
Plinho Francisco
PF
Hertz
D001426:Bacterial Proteins / Q000737:chemistry*; D055162:Biocatalysis; D048271:Chitosan / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005964:Glucosyltransferases / Q000737:chemistry*; D005976:Glutaral / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013213:Starch / Q000737:chemistry*; D013696:Temperature; D046968:Thermoanaerobacter / Q000737:chemistry / Q000201:enzymology; D047392:beta-Cyclodextrins / Q000138:chemical synthesis*
D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D047392:beta-Cyclodextrins; D013213:Starch; D048271:Chitosan; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase; C031215:betadex; D005976:Glutaral
Chitosan; Cyclodextrin glycosyltransferase; Enzyme immobilization; Packed bed reactor