Preparation and characterisation of vancomycin-impregnated gelatin microspheres/silk fibroin scaffold.
A type of antibacterial silk fibroin (SF) scaffold was developed and characterised as a potential functional wound dressing for acute trauma treatment. To achieve this, SF solution was mixed with previously fabricated vancomycin (Vm)-loaded gelatin (G) microspheres, followed by a freeze-drying step. Some physical and antimicrobial properties of the prepared Vm/G/SF scaffolds were investigated and the results demonstrated that the average pore size and porosity of the composite scaffold were 60-80 μm and 75%, respectively. The compressive stress and compressive modulus of Vm/G/SF scaffold were 140 and 468 KPa, respectively. Compared with Vm/G microspheres and Vm/SF scaffold, the Vm/G/SF scaffold has slower release rate of Vm. In addition, the Vm release rate of Vm/G/SF scaffold matched well with the degradation rate of SF scaffold. With respect to the antimicrobial effect, the results showed that the Vm/G/SF scaffold had good antimicrobial activity against Staphylococcus aureus (gram-positive), which is a gram-positive bacteria commonly found in infected wounds.
a Key Laboratory of Biomaterials of Guangdong Higher Education Institutes , Jinan University , Guangzhou , 510632 , China .
Yong
Y
Lan
Weichang
W
Li
Rui
R
Guo
Yi
Y
Zhang
Wei
W
Xue
Yuanming
Y
Zhang
D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D001458:Bandages; D002417:Cattle; D004337:Drug Carriers / Q000737:chemistry; D005349:Fibroins / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D055595:Mechanical Phenomena; D008863:Microspheres; D013211:Staphylococcus aureus / Q000187:drug effects; D013696:Temperature; D014640:Vancomycin / Q000008:administration & dosage / Q000494:pharmacology*; D014945:Wound Healing
D000890:Anti-Infective Agents; D004337:Drug Carriers; D014640:Vancomycin; D005780:Gelatin; D005349:Fibroins