The ocular consequences and applicability of minimally invasive 25-gauge transvitreal retinochoroidal biopsy.
OBJECTIVE
To determine the applicability and ocular morbidity of the 25-gauge transvitreal retinochoroidal biopsy technique in the management of intraocular tumors.


METHODS
Retrospective, consecutive, observational, single-surgeon case series.


METHODS
A total of 124 biopsies were performed in 123 patients with intraocular tumors in the posterior segment from January 1, 2009, through December 31, 2011.


METHODS
The biopsies were performed under general anesthesia with standard 25-gauge vitrectomy equipment. The vitreous body and the retinotomies were left untreated with the exception of 1 patient in whom a complete vitrectomy and oil tamponade were performed. Histopathologic examination of all samples was performed and cytogenetic testing with fluorescence in situ hybridization and multiplex ligation-dependent probe amplification were performed in the uveal melanomas. Median follow-up time was 26.3 months (range, 2.0-47.2 months).


METHODS
Histopathologic diagnosis and chromosome 3 analysis of the biopsy-obtained tissue sample. Clinical observations included visual acuity, retinal detachment, vitreous hemorrhage, and secondary enucleation.


RESULTS
Histopathologic diagnosis was obtained in 97.6% (n = 121) of the intraocular tumors, and chromosome 3 status could be determined in 97.3% (n = 110) of uveal melanoma patients. Preoperative retinal detachment was present in 65% (n = 55). Apart from in 1 case, all retinal detachments remained stable during surgery. Additionally, 7.1% (n = 6) of cases demonstrated retinal detachment during the follow-up period, and vitreous hemorrhage was observed in 96.5% of cases (n = 82) 1 day after surgery. Both conditions regressed spontaneously in nearly all cases. Retinal detachment surgery and vitrectomy resulting from persistent vitreous hemorrhage was performed in 3.5% (n = 3) and 5.9% (n = 5) of patients, respectively. The frequency of secondary enucleated eyes was 6.7% (n = 5). Free tumor cells after biopsy were described in 15.9% (n = 7), but no tumor recurrence at the sclerotomy sites was observed. A decrease in visual acuity from better than 0.1 (20/200) at diagnosis to 0.1 or worse at 1 and 3 years of follow-up was observed in 21.7% (n = 13) and 41.7% (n = 5) of patients, respectively.


CONCLUSIONS
The 25-gauge transvitreal retinochoroidal biopsy provides a large sample, adequate for histopathologic examination and cytogenetic analysis. The procedure is associated with a low risk of ocular complications.
Department of Ophthalmology, Rigshospitalet and Glostrup Hospital, Copenhagen University Hospital, Copenhagen, Denmark; Department of Ophthalmology, Roskilde Hospital, Copenhagen University Hospital, Roskilde, Denmark. Electronic address: bagger.mette@gmail.com.
Mette
M
Bagger
Department of Ophthalmology, Rigshospitalet and Glostrup Hospital, Copenhagen University Hospital, Copenhagen, Denmark.
Jens F
JF
Tebering
Department of Ophthalmology, Rigshospitalet and Glostrup Hospital, Copenhagen University Hospital, Copenhagen, Denmark.
Jens F
JF
Kiilgaard
D044963:Biopsy, Fine-Needle / Q000379:methods*; D002829:Choroid / Q000473:pathology; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics; D015353:Eye Enucleation; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008545:Melanoma / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D008875:Middle Aged; D019060:Minimally Invasive Surgical Procedures; D060885:Multiplex Polymerase Chain Reaction; D009339:Needles; D009367:Neoplasm Staging; D012160:Retina / Q000473:pathology; D012163:Retinal Detachment / Q000175:diagnosis; D012189:Retrospective Studies; D014604:Uveal Neoplasms / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D014792:Visual Acuity / Q000502:physiology; D014821:Vitrectomy / Q000295:instrumentation; D014823:Vitreous Hemorrhage / Q000175:diagnosis; D000098943:Uveal Melanoma