[Study on quality evaluation of cimicifugae rhizoma from different producing areas by HPLC fingerprint].
To establish a fingerprint for Cimicifugae Rhizoma from different producing areas. Column kromasil (4.6 mm x 250 mm, 5 microm) was employed with acetonitrile-0.1% formic acid solution as the mobile phase for gradient elution. The flow rate was 1.0 mL x min(-1), the detection wavelength was 254 nm. Twenty chromatographic peaks were extracted as the common peaks of fingerprint, and 21 batches of samples were compared and classified with such methods as similarity evaluation, cluster analysis and principle component analysis. The results showed 12 common peaks and three categories of samples. The method was so highly reproducible, simple and reliable that it could provide basis for quality control and evaluation of Cimicifugae Rhizoma from different producing areas.
Engineering Research Center for Processing Standardization of Traditional Chinese Medicines, State Ministry of Education, Nanjing University of Chinese Medicine, Nanjing 210029, China. shenbaojia@163.com
Bao-Jia
BJ
Shen
Kun-Ming
KM
Qin
Xing-Hai
XH
Zhang
Qi-Di
QD
Liu
Hao
H
Cai
Xiao
X
Liu
Bao-Chang
BC
Cai
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031885:Cimicifuga / Q000737:chemistry*; D004355:Drug Stability; D011786:Quality Control