[Detection of the major milk allergen alpha-casein in food with liquid chromatography-tandem mass spectrometry in multiple reaction monitoring mode].
A new method of detecting milk allergen protein in food was established. Using multiple reaction monitoring (MRM) mode in high performance liquid chromatography-mass spectrometry (HPLC-MS) to detect milk allergen protein alpha-casein is successful and convenient. All transitions gave a linear response in the chosen concentration range of the milk allergen protein (between 0. 5 and 250 mg/L total soluble protein, 5 data points), and the detection limit is 0.5 mg/L of alpha-casein. Food samples from markets were tested. The results showed that MRM method could be used to detect the milk allergen in food products.
Department of Chemistry, Fudan University, Shanghai 200433, China.
Xiaoyan
X
Feng
Jin
J
Zhang
Meiling
M
Lü
Mingxia
M
Gao
Xiangmin
X
Zhang
D000485:Allergens / Q000032:analysis*; D000818:Animals; D002364:Caseins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D057230:Limit of Detection; D008892:Milk; D053719:Tandem Mass Spectrometry
D000485:Allergens; D002364:Caseins