A glyco-gold nanoparticle based assay for α-2,8-polysialyltransferase from Neisseria meningitidis.
We designed a novel strategy for sensitively detecting the activity of α-2,8-polysialyltransferase (PST) by a combination of ganglioside GD3 functionalized gold nanoparticles and inactive endosialidase. We anticipate that this new method will facilitate the search for PST inhibitors as well as for improved mutant forms of PST in directed evolution experiments.
Department of Chemistry, National Tsing Hua University, 101, Sec. 2, Kuang Fu Rd., Hsinchu, 30013, Taiwan. cclin66@mx.nthu.edu.tw.
Ching-Ching
CC
Yu
Li-De
LD
Huang
David H
DH
Kwan
Warren W
WW
Wakarchuk
Stephen G
SG
Withers
Chun-Cheng
CC
Lin
D015374:Biosensing Techniques / Q000379:methods*; D005732:Gangliosides / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D008954:Models, Biological; D008958:Models, Molecular; D009345:Neisseria meningitidis / Q000201:enzymology*; D012799:Sialyltransferases / Q000737:chemistry* / Q000378:metabolism
D005732:Gangliosides; D006046:Gold; D012799:Sialyltransferases