Medium optimization, molecular characterization, and bioactivity of exopolysaccharides from Pleurotus eryngii.
An optimal medium for exopolysaccharides (EPS) production was obtained through one-factor-at-a-time method and response surface methodology. Under optimal culture medium, the maximum EPS concentration in shake flask was 5.16 g/l. Two groups of EPSs (designated as Fr-I and Fr-II) were obtained from the culture filtrates by size exclusion chromatography/multiangle laser light scattering, and the weight average molar masses (M w) of Fr-I and Fr-II were determined to be 4.098 × 10(4) and 1.114 × 10(4) g/mol, respectively. The molecular confirmation of Fr-I was revealed to be a rigid rod form in aqueous solution. Moreover, monosaccharide composition and characteristic groups were investigated by GC and Fourier transform infrared, respectively. Finally, pharmacology experiment in vitro indicated EPS Fr-II of Pleurotus eryngii exhibited higher antioxidant and antitumor abilities than Fr-I, which might be attributed to the different molecular weights and chemical compositions in the EPS fraction.
College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450002, Henan, People's Republic of China.
Xiaoyan
X
Jing
Duobin
D
Mao
Lujing
L
Geng
Chunping
C
Xu
D000970:Antineoplastic Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D002845:Chromatography; D003470:Culture Media / Q000737:chemistry; D062610:Fungal Polysaccharides / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D008970:Molecular Weight; D020076:Pleurotus / Q000737:chemistry* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared
D000970:Antineoplastic Agents; D000975:Antioxidants; D003470:Culture Media; D062610:Fungal Polysaccharides