title: Effects of vitamin E supplementation on cellular α-tocopherol concentrations of neutrophils in Holstein calves.
abstract: The effects of vitamin E supplementation on cellular α-tocopherol concentrations of neutrophils from Holstein calves and the mechanism of scavenger receptor class B type I (SR-BI)-mediated uptake of α-tocopherol were examined. Cellular α-tocopherol concentrations in vitamin E-treated calves increased from 3.5 ± 0.38 to 7.2 ± 0.84 μg/10(7) cells, respectively, within 14 d after vitamin E supplementation; these concentrations were significantly higher than those of control calves (P < 0.01). The expression indices of SR-BI [a major receptor that recognizes high-density lipoprotein (HDL)] mRNA in neutrophils were two to five times higher (P < 0.01) in neutrophils obtained from vitamin E-supplemented calves compared with those from control calves, and anti-SR-B1 antibody, ranging from 0.1 to 1.0 μg/mL, significantly (P < 0.01) decreased cellular α-tocopherol concentrations of neutrophils. Cytochalasin D and latrunculin B, major inhibitors of actin polymerization of neutrophils, significantly decreased cellular α-tocopherol concentrations of neutrophils (P < 0.01). Our results demonstrated that in vitamin E-supplemented calves: 1) α-tocopherol is mainly distributed with HDL, 2) α-tocopherol within HDL is recognized by SR-BI on the surface of neutrophils, and 3) rearrangement of the actin cytoskeleton is a crucial step for the uptake of α-tocopherol by neutrophils.
authors.affiliation: Animal Health Laboratory (Higuchi, Ito, Nagahata), Department of Veterinary Herd Health (Oikawa), Department of Health and Environmental Sciences and Veterinary Biochemistry, and Department of Bioscience (Iwano), School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Higuchi
authors.forename: Erina
authors.initials: E
authors.lastname: Ito
authors.forename: Hidetoma
authors.initials: H
authors.lastname: Iwano
authors.forename: Shin
authors.initials: S
authors.lastname: Oikawa
authors.forename: Hajime
authors.initials: H
authors.lastname: Nagahata
mesh_terms: D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D008076:Cholesterol, HDL / Q000235:genetics / Q000378:metabolism; D003126:Colostrum; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D005786:Gene Expression Regulation / Q000187:drug effects; D009504:Neutrophils / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051122:Scavenger Receptors, Class B / Q000235:genetics / Q000378:metabolism; D014810:Vitamin E / Q000008:administration & dosage* / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008076:Cholesterol, HDL; D012333:RNA, Messenger; D051122:Scavenger Receptors, Class B; D014810:Vitamin E; D024502:alpha-Tocopherol