title: Anti-inflammatory response of dietary vitamin E and its effects on pain and joint structures during early stages of surgically induced osteoarthritis in dogs.
abstract: There is evidence that vitamin E (VE) has anti-inflammatory and analgesic properties in human osteoarthritis (OA). This double-blinded and randomized pilot study used a broad spectrum of clinical and laboratory parameters to investigate whether such beneficial effects could be detected in a canine experimental OA model. Dogs were divided into 2 groups: control (n = 8), which received a placebo, and test group (n = 7), which received 400 IU/animal per day of VE for 55 d, starting the day after transection of the cranial cruciate ligament. Lameness and pain were assessed using a visual analogue scale (VAS), numerical rating scale (NRS), and electrodermal activity (EDA) at day 0, day 28, and day 55. Cartilage and synovial inflammation lesions were assessed. One-side comparison was conducted at an alpha-threshold of 10%. At day 56, dogs were euthanized and concentrations of prostaglandin E2 (PGE2), nitrogen oxides (NOx), and interleukin-1 beta (IL-1β) were measured in synovial fluid. Concentrations of NOx and PGE2 in synovial fluid were lower in the test group (P < 0.0001 and P = 0.03, respectively). Values of VAS, NRS, and EDA showed a consistent trend to be lower in the test group than in the control, while statistical significance was reached for VAS at day 55 and for EDA at day 28 (adjusted P = 0.07 in both cases). Histological analyses of cartilage showed a significant reduction in the scores of lesions in the test group. This is the first time that a study in dogs with OA using a supplement with a high dose of vitamin E showed a reduction in inflammation joint markers and histological expression, as well as a trend to improving signs of pain.
authors.affiliation: Research Group in Animal Pharmacology of Quebec (GREPAQ), Département de biomédecine vétérinaire (Rhouma, Troncy, Beaudry, Chorfi) and Département de sciences cliniques (El Warrak), Faculté de médecine vétérinaire, Université de Montréal, 3200 Sicotte Street, P.O. Box 5000, Saint-Hyacinthe, Quebec J2S 7C6.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Rhouma
authors.forename: Alexander
authors.initials: A
authors.lastname: de Oliveira El Warrak
authors.forename: Eric
authors.initials: E
authors.lastname: Troncy
authors.forename: Francis
authors.initials: F
authors.lastname: Beaudry
authors.forename: Younès
authors.initials: Y
authors.lastname: Chorfi
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000276:immunology* / Q000473:pathology; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D015232:Dinoprostone / Q000032:analysis; D004283:Dog Diseases / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology; D004285:Dogs; D004311:Double-Blind Method; D006651:Histocytochemistry / Q000662:veterinary; D007249:Inflammation / Q000188:drug therapy / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D053583:Interleukin-1beta / Q000032:analysis; D007794:Lameness, Animal / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology; D009589:Nitrogen Oxides / Q000032:analysis; D010003:Osteoarthritis / Q000188:drug therapy / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D010146:Pain / Q000188:drug therapy / Q000662:veterinary; D010865:Pilot Projects; D011897:Random Allocation; D013582:Synovial Fluid / Q000737:chemistry / Q000276:immunology; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D053583:Interleukin-1beta; D009589:Nitrogen Oxides; D014810:Vitamin E; D015232:Dinoprostone