title: Frequency domain fluorometry: theory and application.
abstract: Frequency domain fluorometry is a widely utilized tool in the physical, chemical, and biological sciences. This chapter focuses on the theory of the method and the practical aspects required to carry out intensity decay, i.e., lifetime measurements on a modern frequency domain fluorometer. Several chemical/biological systems are utilized to illustrate data acquisition protocols. Data analysis procedures and methodologies are also discussed.
authors.affiliation: Department Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, USA.
authors.forename: Carissa M
authors.initials: CM
authors.lastname: Vetromile
authors.forename: David M
authors.initials: DM
authors.lastname: Jameson
mesh_terms: D005453:Fluorescence; D005470:Fluorometry / Q000379:methods*; D008958:Models, Molecular; D008967:Molecular Biology / Q000379:methods*