title: Antimicrobial resistance and detection of the mecA gene besides enterotoxin-encoding genes among coagulase-negative Staphylococci isolated from clam meat of Anomalocardia brasiliana.
abstract: The marine clam Anomalocardia brasiliana is a candidate as a sentinel animal to monitor the contamination levels of coliforms in shellfish-harvesting areas of Brazil's northeastern region. The aim of the present study was to search enterotoxin-encoding genes plus the mecA gene among coagulase-negative staphylococci (CNS) isolates from shellfish meats of A. brasiliana. The specimen clam (n=48; 40 clams per sample) was collected during low tide in the bay area of Mangue Seco from April through June 2009, and random samples of chilled and frozen shelled clam meat (n=33; 250 g per sample) were obtained from retail shops from January through March 2012. Seventy-nine CNS isolates were identified, including Staphylococcus xylosus, S. cohnii spp. urealyticus, S. sciuri, and S. lentus. A high percentage of isolates resistant to erythromycin (58.5%), penicillin (51.2%), and tetracycline (43.9%), and the fluoroquinolones levofloxacin (39%) and ciprofloxacin (34.1%) were recorded from those environmental samples. Isolates from retail shops were particularly resistant to oxacillin (55.3%) and penicillin (36.8%). All CNS resistant to oxacillin and/or cefoxitin were positive for the presence of the mecA gene, but phenotypically susceptible to vancomycin. Also, the enterotoxin-encoding genes seg and seh were detected through multiplex-polymerase chain reaction in 77.7% and 88.8% of the isolates from environmental samples, versus 90.5% and 100% of the isolates from retail shops, respectively. The data reveal the risk to public health due to consuming raw or undercooked shellfish containing enterotoxigenic plus methicillin-resistant CNS.
authors.affiliation: 1 Laboratory of Microbiology and Immunology, Department of Biology, Federal Rural University of Pernambuco , Recife, Pernambuco, Brazil .
authors.forename: Jacqueline Ellen Camelo
authors.initials: JE
authors.lastname: Batista
authors.forename: Ewerton Lucena
authors.initials: EL
authors.lastname: Ferreira
authors.forename: Danielle Cristina de Oliveira
authors.initials: DC
authors.lastname: Nascimento
authors.forename: Roberta Ferreira
authors.initials: RF
authors.lastname: Ventura
authors.forename: Wagner Luis Mendes
authors.initials: WL
authors.lastname: de Oliveira
authors.forename: Nilma Cintra
authors.initials: NC
authors.lastname: Leal
authors.forename: José Vitor
authors.initials: JV
authors.lastname: Lima-Filho
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D049872:Bivalvia / Q000382:microbiology*; D001938:Brazil; D003030:Coagulase / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004768:Enterotoxins / Q000235:genetics*; D005506:Food Contamination; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D012758:Shellfish / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000382:microbiology*; D013210:Staphylococcus / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D003030:Coagulase; D004768:Enterotoxins