title: The role of Vitamin D level and related single nucleotide polymorphisms in Crohn's disease.
abstract: New Zealand has one of the highest rates of Crohn's Disease (CD) in the world, and there is much speculation as to why this might be. A high risk of CD has been associated with deficient or insufficient levels of Vitamin D (Vit D), lifestyle as well as various genetic polymorphisms. In this study we sought to analyse the relevance of serum Vit D levels, lifestyle and genotype to CD status. Serum samples were analysed for 25-OH-Vitamin D levels. DNA was isolated from blood and cheek-swabs, and Sequenom and ImmunoChip techniques were used for genotyping. Serum Vit D levels were significantly lower in CD patients (mean = 49.5 mg/L) than those found in controls (mean = 58.9 mg/L, p = 4.74 × 10⁻⁶). A total of seven single nucleotide polymorphisms were examined for effects on serum Vit D levels, with adjustment for confounding variables. Two variants: rs731236[A] (VDR) and rs732594[A] (SCUBE3) showed a significant association with serum Vit D levels in CD patients. Four variants: rs7975232[A] (VDR), rs732594[A] (SCUBE3), and rs2980[T] and rs2981[A] (PHF-11) showed a significant association with serum Vit D levels in the control group. This study demonstrates a significant interaction between Vit D levels and CD susceptibility, as well as a significant association between Vit D levels and genotype.
authors.affiliation: Presbyterian University Mackenzie, R. da Consolacao 930, Sao Paulo 01302-907, Brazil. l.ferguson@auckland.ac.nz.
authors.forename: Andre Y O M
authors.initials: AY
authors.lastname: Carvalho
authors.forename: Karen S
authors.initials: KS
authors.lastname: Bishop
authors.forename: Dug Yeo
authors.initials: DY
authors.lastname: Han
authors.forename: Stephanie
authors.initials: S
authors.lastname: Ellett
authors.forename: Amalini
authors.initials: A
authors.lastname: Jesuthasan
authors.forename: Wen J
authors.initials: WJ
authors.lastname: Lam
authors.forename: Lynnette R
authors.initials: LR
authors.lastname: Ferguson
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002135:Calcium-Binding Proteins / Q000235:genetics; D016022:Case-Control Studies; D002648:Child; D003424:Crohn Disease / Q000097:blood* / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008019:Life Style; D008297:Male; D008875:Middle Aged; D009520:New Zealand; D020641:Polymorphism, Single Nucleotide; D018167:Receptors, Calcitriol / Q000235:genetics; D014807:Vitamin D / Q000097:blood*; D055815:Young Adult
chemical_list: D002135:Calcium-Binding Proteins; D018167:Receptors, Calcitriol; C493800:SCUBE3 protein, human; C579457:VDR protein, human; D014807:Vitamin D