title: Enhanced differentiation of rat MSCs into cardiomyocytes with 5-azacytidine/collagen I nano-molecules.
abstract: This study was to investigate the enhancement ability of 5-azacytidine (5-aza) and collagen I nano-molecules treatment to the differentiation of rat mesenchymal stem cells (MSCs) towards a cardiomyocytes in vitro. The results demonstrated that the size of the cells increased significantly and connecting with adjoining cells by forming myotube-like structures. Also, additional treatment of the MSCs with collagen I nano-fibrils significantly increased two transcription factors GATA-4 and Nkx2.5 expressions and three expressions of cardiac genes of troponin I, β-myosin heavy chain and cardiac α-actin compared with MSC groups treated only with 5-aza at early 3 d culturing(all, P<0.01 or better). These results indicate that culturing MSCs with collagen I nano-molecules, which could act as scaffolds or soluble protein ingredients, leads to alterations in gene expression and affects the differentiation fate induced with 5-aza.
authors.forename: Yi-Jhen
authors.initials: YJ
authors.lastname: Wu
authors.forename: Shu Ying
authors.initials: SY
authors.lastname: Chen
authors.forename: Shwu Jen
authors.initials: SJ
authors.lastname: Chang
authors.forename: Shyh Ming
authors.initials: SM
authors.lastname: Kuo
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001374:Azacitidine / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000494:pharmacology* / Q000648:ultrastructure; D003470:Culture Media; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000502:physiology*; D018995:Myosin Heavy Chains / Q000378:metabolism; D057139:Nanofibers / Q000648:ultrastructure; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds
chemical_list: D000199:Actins; D024042:Collagen Type I; D003470:Culture Media; D018995:Myosin Heavy Chains; D001374:Azacitidine