title: Gene expression profiles of sodium-dependent vitamin C transporters in mice after alcohol consumption.
abstract: Alcoholic liver disease (ALD) is a serious liver problem in western countries. Our previous study has demonstrated that vitamin C plays a protective role in ALD. The vitamin C homeostasis is tightly regulated by sodium-dependent vitamin C transporters (SVCTs) 1 and 2. But the role of two SVCTs in ALD is less understood. In this study, we examined the expression patterns of two SVCTs in mice after alcohol consumption. Our results suggested that alcohol consumption obviously increased the expression of two SVCTs in liver and SVCT1 in kidney and intestine, which is important for vitamin C absorption. Vitamin C supplement increased the sera vitamin C content and ameliorated the symptom of ALD. Intestinal absorption and renal re-absorption mediated by SVCT1 are key factors to increase the sera vitamin C content after alcohol consumption. We proposed that both reactive oxygen species and low vitamin C concentration regulate the expression of SVCTs, and the protective role of vitamin C is mediated by suppressing the stability of hypoxia-inducible factor-1α. Thus, our study is significant for the understanding of vitamin C homeostasis in ALD and for better use of other antioxidants in ALD therapy.
authors.affiliation: Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang 050024, China.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Guo
authors.forename: Yuejia
authors.initials: Y
authors.lastname: Wang
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Shen
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Gao
authors.forename: Yanzhong
authors.initials: Y
authors.lastname: Chang
authors.forename: Xianglin
authors.initials: X
authors.lastname: Duan
mesh_terms: D000428:Alcohol Drinking; D000818:Animals; D001205:Ascorbic Acid / Q000097:blood; D001483:Base Sequence; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers; D000431:Ethanol / Q000633:toxicity; D020869:Gene Expression Profiling; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D060888:Real-Time Polymerase Chain Reaction; D060347:Sodium-Coupled Vitamin C Transporters / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D017931:DNA Primers; C497443:Hif1a protein, mouse; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D060347:Sodium-Coupled Vitamin C Transporters; D000431:Ethanol; D001205:Ascorbic Acid
keywords: alcoholic liver disease; iron overload; sodium-dependent vitamin C transporter; vitamin C