title: Patch testing with serial dilutions and thin-layer chromatograms of oak moss absolutes containing high and low levels of atranol and chloroatranol.
abstract: BACKGROUND
  Oak moss absolute (Evernia prunastri extract) contains a large number of substances, among them the potent allergens atranol and chloroatranol. Since 2008, their content in oak moss absolute has been restricted by the International Fragrance Association to a maximum level of 100 ppm each.
  
  
  OBJECTIVE
  To compare the elicitation capacities of a traditional (sample A) and a treated (sample B) oak moss absolute containing, in total, 27 000 and 66 ppm of atranol and chloroatranol, respectively, and to investigate reactions to components of oak moss absolute separated by thin-layer chromatography (TLC).
  
  
  METHODS
  Fifteen oak moss-allergic subjects were patch tested with serial dilutions and TLC strips of samples A and B.
  
  
  RESULTS
  Fifteen subjects reacted to sample A at concentrations ≤ 2.0%, and 2 subjects reacted to sample B at 2.0% but not to lower concentrations. Among 13 subjects reacting to the TLC strip of sample A, 11 reacted to spots with retardation factor values corresponding to those of atranol and/or chloroatranol, and 11 reacted to other areas on the TLC strip. Only one subject reacted to the TLC strip of sample B.
  
  
  CONCLUSIONS
  The patch test reactivity of sample B was significantly lower than that of sample A. The TLC patch tests indicate the presence of sensitizers other than atranol and chloroatranol in oak moss absolute.
authors.affiliation: Department of Occupational and Environmental Dermatology, Skåne University Hospital, Lund University, SE-20502, Malmö, Sweden.
authors.forename: Martin
authors.initials: M
authors.lastname: Mowitz
authors.forename: Erik
authors.initials: E
authors.lastname: Zimerson
authors.forename: Cecilia
authors.initials: C
authors.lastname: Svedman
authors.forename: Magnus
authors.initials: M
authors.lastname: Bruze
mesh_terms: D000328:Adult; D000368:Aged; D000485:Allergens / Q000008:administration & dosage / Q000009:adverse effects; D001547:Benzaldehydes / Q000008:administration & dosage / Q000009:adverse effects; D002855:Chromatography, Thin Layer; D017449:Dermatitis, Allergic Contact / Q000175:diagnosis* / Q000209:etiology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010328:Patch Tests / Q000379:methods*; D012116:Resins, Plant / Q000009:adverse effects* / Q000737:chemistry; D012996:Solutions / Q000008:administration & dosage; D013729:Terpenes / Q000009:adverse effects* / Q000737:chemistry
chemical_list: D000485:Allergens; D001547:Benzaldehydes; D012116:Resins, Plant; D012996:Solutions; D013729:Terpenes; C493895:atranol; C487450:chloroatranol; C044724:oakmoss
keywords: Evernia prunastri extract; atranol; chloroatranol; oak moss absolute