title: Subcytolytic effects of suilysin on interaction of Streptococcus suis with epithelial cells.
abstract: Suilysin is a pore-forming cholesterol-dependent cytolysin secreted by Streptococcus suis (S. suis), an important swine and zoonotic pathogen. The role of suilysin in S. suis host-cell interaction is still unclear. We found a higher adherence and invasion rate of an unencapsulated sly-positive strain in comparison to its isogenic sly-negative mutant. Electron microscopy revealed that formation of membrane ruffles accompanying invasion of the sly-positive strain was abolished in the sly-negative mutant. Inhibition experiments showed that the actin cytoskeleton was involved in suilysin-mediated effects. Point-mutation of the domain putatively responsible for macropore-formation resulted in abolished hemolytic and cytolysin activity, but had no effect on S. suis host cell association. Concluding, our results indicate that subcytolytic suilysin promotes S. suis association with epithelial cells.
authors.affiliation: Institute for Microbiology, University of Veterinary Medicine Hannover, Hannover, Germany. Electronic address: maren.seitz@tiho-hannover.de.
authors.forename: M
authors.initials: M
authors.lastname: Seitz
authors.forename: C G
authors.initials: CG
authors.lastname: Baums
authors.forename: C
authors.initials: C
authors.lastname: Neis
authors.forename: L
authors.initials: L
authors.lastname: Benga
authors.forename: M
authors.initials: M
authors.lastname: Fulde
authors.forename: M
authors.initials: M
authors.lastname: Rohde
authors.forename: R
authors.initials: R
authors.lastname: Goethe
authors.forename: P
authors.initials: P
authors.lastname: Valentin-Weigand
mesh_terms: D000818:Animals; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology; D006460:Hemolysin Proteins / Q000494:pharmacology* / Q000633:toxicity; D054884:Host-Pathogen Interactions / Q000187:drug effects*; D006801:Humans; D009154:Mutation; D013290:Streptococcal Infections / Q000382:microbiology*; D017011:Streptococcus suis / Q000187:drug effects* / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D006460:Hemolysin Proteins; C104760:suilysin
keywords: Cholesterol-dependent cytolysin; Streptococcus suis; Suilysin