title: Influence of sinomenine on protein profiles of peripheral blood mononuclear cells from ankylosing spondylitis patients: a pharmacoproteomics study.
abstract: BACKGROUND
  Ankylosing spondylitis (AS) is a common inflammatory rheumatic disease which lacks satisfactory treatment so far. Sinomenine (SIN) is an alkaloid and has recently been utilized in treating multiple rheumatic diseases including AS in China, but its exact mechanism remains to be explored. This study investigated the alteration of proteome in peripheral blood mononuclear cells (PBMCs) from AS patients.
  
  
  METHODS
  Thirty AS patients were enrolled in this study. PBMCs from each AS patient were cultured in medium with or without SIN respectively. Then PBMCs proteins from both groups were separated by two-dimensional electrophoresis (2-DE) and analyzed by mass spectrometry (MS). Two differentially expressed proteins were then chosen to be verified using Western blotting.
  
  
  RESULTS
  Seven proteins, including a-synuclein (SNCA), calmodulin (CALM), acidic leucine-rich nuclear phosphoprotein 32 family member A (ANP32A), chloride intracellular channel protein 1 (CLIC1), guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (GNB1), gelsolin (GSN) and histone H2B type 1-M (HISTH2BM) were over-expressed, while coronin- 1A (CORO1A) was under-expressed in the SIN-treated PBMCs. Further bioinformatics search indicated that the changes of SNCA, ANP32A and CLIC1 pertained to apoptosis, while changes of GSN and CORO1A were associated with both apoptosis and inhibition of immunological function. Subsequently GSN and CORO1A were selected to validate by Western blotting and the results were consistent with those of 2-DE.
  
  
  CONCLUSIONS
  There were 8 differentially expressed proteins in the SIN-treated PBMCs, which might shed some light on the mechanism of SIN in the treatment of AS.
authors.affiliation: Department of Rheumatology and Immunology, Guangdong No. 2 Provincial People's Hospital, Guangzhou, Guangdong 510317, China.
authors.forename: Zhi-xiang
authors.initials: ZX
authors.lastname: Huang
authors.forename: Jin-hui
authors.initials: JH
authors.lastname: Tan
authors.forename: Tian-wang
authors.initials: TW
authors.lastname: Li
authors.forename: Wei-ming
authors.initials: WM
authors.lastname: Deng
authors.forename: Ke-wei
authors.initials: KW
authors.lastname: Qiu
authors.forename: Ze-tao
authors.initials: ZT
authors.lastname: Liao
authors.forename: Zhao-qiu
authors.initials: ZQ
authors.lastname: Zeng
mesh_terms: D000293:Adolescent; D000328:Adult; D001798:Blood Proteins / Q000032:analysis*; D015153:Blotting, Western; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D009019:Morphinans / Q000494:pharmacology*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D013167:Spondylitis, Ankylosing / Q000097:blood*
chemical_list: D001798:Blood Proteins; D009019:Morphinans; C009271:sinomenine