title: Autophagy prevents irradiation injury and maintains stemness through decreasing ROS generation in mesenchymal stem cells.
abstract: Stem cells were characterized by their stemness: self-renewal and pluripotency. Mesenchymal stem cells (MSCs) are a unique type of adult stem cells that have been proven to be involved in tissue repair, immunoloregulation and tumorigenesis. Irradiation is a well-known factor that leads to functional obstacle in stem cells. However, the mechanism of stemness maintenance in human MSCs exposed to irradiation remains unknown. We demonstrated that irradiation could induce reactive oxygen species (ROS) accumulation that resulted in DNA damage and stemness injury in MSCs. Autophagy induced by starvation or rapamycin can reduce ROS accumulation-associated DNA damage and maintain stemness in MSCs. Further, inhibition of autophagy leads to augment of ROS accumulation and DNA damage, which results in the loss of stemness in MSCs. Our results indicate that autophagy may have an important role in protecting stemness of MSCs from irradiation injury.
authors.affiliation: 1] Tumor Immunology and Gene Therapy Center, Eastern Hepatobiliary Surgery Hospital, the Second Military Medical University, Shanghai, China [2] Department of Pharmacy, Changhai Hospital, The Second Military Medical University, Shanghai, China.
authors.forename: J
authors.initials: J
authors.lastname: Hou
authors.forename: Z-p
authors.initials: ZP
authors.lastname: Han
authors.forename: Y-y
authors.initials: YY
authors.lastname: Jing
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.forename: S-s
authors.initials: SS
authors.lastname: Zhang
authors.forename: K
authors.initials: K
authors.lastname: Sun
authors.forename: C
authors.initials: C
authors.lastname: Hao
authors.forename: Y
authors.initials: Y
authors.lastname: Meng
authors.forename: F-h
authors.initials: FH
authors.lastname: Yu
authors.forename: X-q
authors.initials: XQ
authors.lastname: Liu
authors.forename: Y-f
authors.initials: YF
authors.lastname: Shi
authors.forename: M-c
authors.initials: MC
authors.lastname: Wu
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: L-x
authors.initials: LX
authors.lastname: Wei
mesh_terms: D000328:Adult; D001343:Autophagy / Q000187:drug effects / Q000528:radiation effects*; D004249:DNA Damage; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000473:pathology* / Q000528:radiation effects* / Q000648:ultrastructure; D020011:Protective Agents / Q000494:pharmacology; D011839:Radiation, Ionizing; D017382:Reactive Oxygen Species / Q000378:metabolism*; D020123:Sirolimus / Q000494:pharmacology; D014617:Vacuoles / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure
chemical_list: D020011:Protective Agents; D017382:Reactive Oxygen Species; D020123:Sirolimus