title: Biosynthesis of terephthalic acid, isophthalic acid and their derivatives from the corresponding dinitriles by tetrachloroterephthalonitrile-induced Rhodococcus sp.
abstract: The nitrilase from Rhodococcus sp. CCZU10-1 catalyses the hydrolysis of dinitriles to acids without the formation of amides and cyanocarboxylic acids. It was induced by benzonitrile and its analogues (tetrachloroterephthalonitrile > ε-caprolactam > benzonitrile > phenylacetonitrile), and had activity towards aromatic nitriles (terephthalonitrile > tetrachloroterephthalonitrile > isophthalonitrile > tetrachloroisophthalonitrile > tetrafluoroterephthalonitrile > benzonitrile). After the optimization, the highest nitrilase induction [311 U/(g DCW)] was achieved with tetrachloroterephthalonitrile (1 mM) in the medium after 24 h at 30 °C after optimum enzyme activity was at pH 6.8 and at 30 °C. Efficient biocatalyst recycling was achieved by cell immobilization in calcium alginate, with a product-to-biocatalyst ratios of 776 g terephthalic acid/g DCW and 630 g isophthalic acid/g DCW.
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical and Life Sciences, Changzhou University, Changzhou, 213164, China, heyucai2001@163.com.
authors.forename: Yu-Cai
authors.initials: YC
authors.lastname: He
authors.forename: Ya-Dong
authors.initials: YD
authors.lastname: Wu
authors.forename: Xue-He
authors.initials: XH
authors.lastname: Pan
authors.forename: Cui-Luan
authors.initials: CL
authors.lastname: Ma
mesh_terms: D000619:Aminohydrolases / Q000378:metabolism*; D002722:Chlorobenzenes / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009570:Nitriles / Q000378:metabolism*; D010795:Phthalic Acids / Q000378:metabolism*; D012240:Rhodococcus / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D015533:Transcriptional Activation
chemical_list: D002722:Chlorobenzenes; D003470:Culture Media; D009570:Nitriles; D010795:Phthalic Acids; C059768:isophthalate; C011363:terephthalic acid; D000619:Aminohydrolases; C021867:nitrilase