title: Adipose-derived regenerative cell (ADRC)-enriched fat grafting: optimal cell concentration and effects on grafted fat characteristics.
abstract: BACKGROUND
  To overcome the absorption of traditional fat grafting, techniques for adipose-derived regenerative cell (ADRC)-enriched fat grafting are currently being adapted for practical application. The Celution800/CRS (Cytori Therapeutics, San Diego, CA) has enabled rapid grafting of the patient's own freshly harvested ADRCs without requiring a culturing step. However, the optimal cell concentration and the effects of ADRCs on the characteristics of grafted fat after free fat grafting remain unclear.
  
  
  METHODS
  ADRCs were isolated and purified from human fat tissue using the Celution800/CRS. Animals that received fat grafting without the addition of ADRCs were designated the control group (group A). The number of ADRCs per grafted fat volume (mL) was adjusted to 3 × 10⁵, 1.5 × 10⁶, and 3 × 10⁶ cells/mL (groups B, C, and D, respectively), mixed with free fat, and transplanted as ADRC-enriched fat grafting. These mixtures were transplanted subcutaneously into BALB/C Jcl-nu/nu mice. The volume of grafted fat was determined 5 months after transplantation, and histological assessments were performed.
  
  
  RESULTS
  ADRC-enriched fat grafting resulted in decreased fat absorption and the formation of greater numbers of new blood vessels in the grafted fat. The optimal ADRC concentration in this study was found to be 3 × 105 cells/mL (group B), with higher concentrations resulting in increased cyst and fibril formation in the grafted fat.
  
  
  CONCLUSIONS
  This study used the Celution800/CRS for free fat grafting and demonstrated that the concentration of transplanted ADRCs affected the engraftment and quality of the grafted fat.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Kansai Medical University, Osaka, Japan. kakudon@hirakata.kmu.ac.jp.
authors.forename: Natsuko
authors.initials: N
authors.lastname: Kakudo
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Naoki
authors.initials: N
authors.lastname: Morimoto
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ogawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kushida
authors.forename: Tomoya
authors.initials: T
authors.lastname: Hara
authors.forename: Kenji
authors.initials: K
authors.lastname: Kusumoto
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000637:transplantation*; D000273:Adipose Tissue / Q000098:blood supply / Q000166:cytology* / Q000637:transplantation*; D000818:Animals; D002196:Capillaries; D002452:Cell Count; D002470:Cell Survival; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D012867:Skin; D013194:Staining and Labeling; D014022:Tissue Survival; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D014841:von Willebrand Factor