title: [Effect of NF-κB on the expression of interleukin-6 induced by lipopolysaccharides of Porphyromonas endodontalis in MC3T3-E1 cells].
abstract: OBJECTIVE
  To investigate the effect of NF-κB signaling on the expression of interleukin-6(IL-6) induced by lipopolysaccharides(LPS) extracted from Porphyromonas endodontalis(P.e) in MC3T3-El cells.
  
  
  METHODS
  MC3T3-E1 cells were pretreated with BAY-117082 for 1 h, and then were treated with 10 mg/L P.e-LPS for different times. The translocation of NF-κB was observed by immunofluorescence. The expression of IL-6 was detected by reverse transcription polymerse chain reaction (RT-PCR) and enzyme-linked immuno sorbent assay (ELISA). Statistical analysis was performed using multi-way ANOVA and Dunnett's t test with SPSS 13.0 software package.
  
  
  RESULTS
  The staining of NF-κB was mostly in cytoplasm in untreated cells. Rapid translocation of NF-κB into nucleus was observed in the cells stimulated for 30 min and mostly relocalization of NF-κB from nucleus to cytoplasm was observed after 60 min. Pretreatment with 10 μmol/L BAY-117082 for 1h significantly inhibited P.e-LPS-induced translocation of NF-κB .The mRNA and proteins of IL-6 decreased significantly after pretreatment with 10 μmol/L BAY-117082 and the expression of IL-6 proteins was reduced from (774.983±6.585) ng/L to (377.384±14.620) ng/L (P<0.01). The group of treatment with BAY-117082 alone had no significant difference from the blank control group.
  
  
  CONCLUSIONS
  P.e-LPS can induce translocation of NF-κB in mouse osteoblast MC3T3-El, and P.e-LPS may induce the expression of IL-6 in mouse osteoblast through the signaling of NF-κB.
authors.affiliation: Department of Endodontics, School of Stomatology, China Medical University. Shenyang 110002, Liaoning Province;China.E-mail:yuyaqiong1987@126.com.
authors.forename: Ya-qiong
authors.initials: YQ
authors.lastname: Yu
authors.forename: Jia-jie
authors.initials: JJ
authors.lastname: Guo
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Qiu
authors.forename: You
authors.initials: Y
authors.lastname: Lv
authors.forename: Ge
authors.initials: G
authors.lastname: Jia
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
mesh_terms: D000818:Animals; D015850:Interleukin-6; D008070:Lipopolysaccharides; D051379:Mice; D016328:NF-kappa B; D009570:Nitriles; D010006:Osteoblasts; D045202:Porphyromonas endodontalis; D012333:RNA, Messenger; D013450:Sulfones
chemical_list: C434003:3-(4-methylphenylsulfonyl)-2-propenenitrile; D015850:Interleukin-6; D008070:Lipopolysaccharides; D016328:NF-kappa B; D009570:Nitriles; D012333:RNA, Messenger; D013450:Sulfones