title: A liquid-phase-blocking concanavalin A enzyme-linked immunosorbent assay for the detection of antibodies against Newcastle disease virus in serum of free-ranging pigeons.
abstract: A competitive liquid-phase-blocking concanavalin A enzyme-linked immunosorbent assay (LPB-ConA-ELISA) was developed in the current study. The assay used ConA as a capture reagent, and the sera of specific pathogen-free chickens immunized with nonpurified Newcastle disease virus (NDV) suspension as detector antibodies, to detect and quantify specific antiviral antibodies in serum samples from free-ranging pigeons. The comparison between the LPB-ConA-ELISA and the hemagglutination inhibition (HI) test for the detection of antibodies in serum samples from 107 pigeons showed significant correlation between the assays (r = 0.875), a high sensitivity (100%), specificity (95.8%), accuracy (96.3%) for the ELISA, and good agreement (κ = 0.83) between the 2 assays. The results of this study suggest that the LPB-ConA-ELISA could be a useful alternative to HI test in the serodiagnosis of NDV in pigeons, or other species of birds.
authors.affiliation: 1Elisabete Schirato de Oliveira, Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Via de Acesso Paulo Donato Castellane, s/n, 14884-900 Jaboticabal, São Paulo, Brazil. elisaschirato@hotmail.com.
authors.forename: Elisabete Schirato
authors.initials: ES
authors.lastname: de Oliveira
authors.forename: Ketherson Rodrigues
authors.initials: KR
authors.lastname: Silva
authors.forename: Filipe Santos
authors.initials: FS
authors.lastname: Fernando
authors.forename: Mariana Costa Mello
authors.initials: MC
authors.lastname: Gonçalves
authors.forename: Camila Cesário
authors.initials: CC
authors.lastname: Fernandes
authors.forename: Mariana Monezi
authors.initials: MM
authors.lastname: Borzi
authors.forename: Romeu Moreira
authors.initials: RM
authors.lastname: dos Santos
authors.forename: Maria de Lourdes Feres
authors.initials: Mde L
authors.lastname: Tamanini
authors.forename: Maria de Fátima da Silva
authors.initials: Mde F
authors.lastname: Montassier
authors.forename: Helio José
authors.initials: HJ
authors.lastname: Montassier
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002645:Chickens; D010856:Columbidae / Q000097:blood / Q000821:virology*; D003208:Concanavalin A; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D016014:Linear Models; D009521:Newcastle Disease / Q000097:blood* / Q000821:virology; D009522:Newcastle disease virus / Q000302:isolation & purification*; D012372:ROC Curve; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013047:Specific Pathogen-Free Organisms
chemical_list: D000914:Antibodies, Viral; D003208:Concanavalin A
keywords: Immunodiagnostic; Newcastle disease virus; lectins; pigeons