title: Haplotype combination of polymorphisms in the ADIPOQ gene promoter is associated with growth traits in Qinchuan cattle.
abstract: Adiponectin modulates lipid and glucose metabolism in adipose tissues and is also related to bone metabolism. Polymorphisms in the ADIPOQ gene likely have an impact on growth traits in cattle. In this study, we examined the relationship between ADIPOQ polymorphisms and body measurement parameters in Chinese beef cattle. First, we sequenced ADIPOQ and 1.2 kb of DNA upstream of its promoter, and we found 14 polymorphisms. With the luciferase reporter assay, we showed that the two polymorphisms SNP PR_-135 A>G and PR_-68 G>C, which are located in the core region of promoter, influence promoter activity of ADIPOQ. Second, we identified three haplotypes involved in these two polymorphic sites: A (A-135/C-68), B (A-135/G-68), and C (G-135/G-68). Haplotypes B and C are major haplotypes in five Chinese populations of cattle (Qinchuan, Nanyang, Jiaxian, Hazakh, and Chinese Holstein). We studied the effects of these three haplotypes on body measurements, gene expression, and promoter activity, and we found that the genotypes are associated with body measurement parameters in Qinchuan cattle. Individuals with genotype BC (AG/GG) had significantly higher body height and heart girth than others, and this result may be interpreted by the following two observations. The promoter activity with haplotype B (A/G) is significantly higher than those with A (A/C) and C (G/G) in driving reporter gene transcription; the ADIPOQ mRNA level in cattle with genotype BC (AG/GG) is relatively lower than that in cattle with genotype BB (AA/GG).
authors.affiliation: a College of Animal Science and Technology, Northwest A & F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, P.R. China.
authors.forename: Liangzhi
authors.initials: L
authors.lastname: Zhang
authors.forename: Mijie
authors.initials: M
authors.lastname: Li
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Lai
authors.forename: Mingjuan
authors.initials: M
authors.lastname: Yang
authors.forename: Yao
authors.initials: Y
authors.lastname: Xu
authors.forename: Liushuai
authors.initials: L
authors.lastname: Hua
authors.forename: Xianyong
authors.initials: X
authors.lastname: Lan
authors.forename: Chunlei
authors.initials: C
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D052242:Adiponectin / Q000235:genetics*; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D049628:Body Size / Q000235:genetics; D001835:Body Weight / Q000235:genetics; D002417:Cattle / Q000235:genetics* / Q000254:growth & development*; D002681:China; D056726:Genetic Association Studies; D005838:Genotype; D006239:Haplotypes; D015810:Linkage Disequilibrium; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D019655:Quantitative Trait, Heritable; D017422:Sequence Analysis, DNA
chemical_list: D052242:Adiponectin