title: Analysis of yeast lipid droplet proteome and lipidome.
abstract: Lipid droplets (LD) are in the spotlight of lipid research because of the link of lipid storage to health and disease and the just incipient understanding of their involvement in cellular processes apart from nonpolar lipid metabolism. Yeast is an excellent model organism to study the lipidome and proteome of LD under different environmental conditions and to address new aspects of LD biology and chemistry. In this chapter, we describe a versatile protocol for the isolation of LD at high purity and address specific demands for handling different yeast species. Moreover, we discuss the analysis of the LD proteome and lipidome based on standard methods such as thin layer chromatography (TLC), gas liquid chromatography (GLC), mass spectrometry (MS) as well as GLC/MS. Finally, we point out similarities and disparities of LD proteome and lipidome from the three different yeasts Saccharomyces cerevisiae, Yarrowia lipolytica, and Pichia pastoris.
authors.affiliation: Institute of Biochemistry, Graz University of Technology, Graz, Austria.
authors.forename: Claudia
authors.initials: C
authors.lastname: Schmidt
authors.forename: Birgit
authors.initials: B
authors.lastname: Ploier
authors.forename: Barbara
authors.initials: B
authors.lastname: Koch
authors.forename: Günther
authors.initials: G
authors.lastname: Daum
mesh_terms: D000818:Animals; D002855:Chromatography, Thin Layer / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D002479:Inclusion Bodies / Q000737:chemistry / Q000378:metabolism*; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry / Q000302:isolation & purification*; D013058:Mass Spectrometry / Q000379:methods; D010843:Pichia; D040901:Proteomics / Q000379:methods*; D012441:Saccharomyces cerevisiae; D025062:Yarrowia
chemical_list: D008055:Lipids
keywords: Fatty acids; Gas liquid chromatography; Lipid droplets/particles; Mass spectrometry; Nonpolar lipids; Phospholipids; Thin layer chromatography; Yeast