title: Antioxidant activity potential of Virginia (flue-cured) tobacco flower polysaccharide fractions obtained by ultrasound-assisted extraction.
abstract: Ultrasound-assisted extraction was employed to extract polysaccharide from Virginia (flue-cured) tobacco flowers. The orthogonal matrix method (L9(3)(4)) was used to determine the optimal extraction conditions as to ultrasound power, extraction time, ratio of solvent to solid, and extraction temperature at 300 W, 4 min, 35 (mL/g), and 70 °C respectively. The crude extract was successively purified by chromatography, yielding two major polysaccharide fractions, termed Fr-I and Fr-II. Both fractions are heteropolysaccharides, mainly containing glucose, mannose, and allose with an a-configuration. Thermo gravimetric analysis (TGA) indicated that the degradation temperatures (Td) of Fr-I and Fr-II were 185 °C and 190 °C respectively. The preliminary antioxidant activity test in vitro showed both fractions could potentialize the scavenging effect on hydroxyl and DPPH radicals in a dose-dependent manner. In conclusion, the two polysaccharides may be useful as naturally potential antioxidant agents for application in food and medicinal fields.
authors.affiliation: College of Food and Biological Engineering, Zhengzhou University of Light Industry.
authors.forename: Chun-Ping
authors.initials: CP
authors.lastname: Xu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xiao
authors.forename: Duo-Bin
authors.initials: DB
authors.lastname: Mao
mesh_terms: D001713:Biphenyl Compounds / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods*; D002845:Chromatography; D035264:Flowers / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification; D017665:Hydroxyl Radical / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D014026:Nicotiana / Q000737:chemistry*; D014465:Ultrasonics
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D011134:Polysaccharides; D012997:Solvents; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl