title: Osteogenic matrix cell sheet transplantation enhances early tendon graft to bone tunnel healing in rabbits.
abstract: The objective of this study was to determine whether osteogenic matrix cell sheets (OMCS) could induce bone formation around grafted tendons, thereby enhancing early stage tendon to bone tunnel healing in skeletally mature male Japanese white rabbits. First, the osteogenic potential of rabbit OMCS was evaluated. Then, the OMCS were transplanted into the interface between the grafted tendon and the bone tunnel created at the tibia. Histological assessments and biomechanical tensile testing were performed after 3 weeks. The rabbit OMCS showed high alkaline phosphatase (ALP) activity, positive staining of ALP, and osteogenic potential when transplanted subcutaneously with beta tricalcium phosphate disks. Newly formed bony walls and positive collagen type I staining were seen around the grafted tendon with OMCS transplantation, whereas such bony walls were thinner or less frequent without OMCS transplantation. Micro-computed tomography images showed significantly higher bone volume in the OMCS transplantation group. The pullout strength was significantly higher with OMCS (0.74 ± 0.23 N/mm(2)) than without OMCS (0.58 ± 0.15 N/mm(2)). These results show that OMCS enhance early tendon to bone tunnel healing. This method can be applied to cases requiring early tendon to bone tunnel healing after ligament reconstruction surgery.
authors.affiliation: Department of Orthopaedic Surgery, Nara Medical University, Shijocho 840, Kashihara, Nara 634-8522, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Inagaki
authors.forename: Kota
authors.initials: K
authors.lastname: Uematsu
authors.forename: Manabu
authors.initials: M
authors.lastname: Akahane
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Morita
authors.forename: Munehiro
authors.initials: M
authors.lastname: Ogawa
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Ueha
authors.forename: Takamasa
authors.initials: T
authors.lastname: Shimizu
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Kura
authors.forename: Kenji
authors.initials: K
authors.lastname: Kawate
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Tanaka
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D016025:Bone Transplantation; D006801:Humans; D008297:Male; D010012:Osteogenesis; D011817:Rabbits; D013710:Tendons / Q000473:pathology / Q000601:surgery / Q000637:transplantation*; D013977:Tibia / Q000601:surgery / Q000637:transplantation*; D014945:Wound Healing
chemical_list: D000469:Alkaline Phosphatase