title: Development of a rapid resolution liquid chromatography-diode array detector method for the determination of three compounds in Ziziphora clinopodioides Lam from different origins of Xinjiang.
abstract: BACKGROUND
  As a traditional Uygur medicinal plant, Z. clinopodioides Lam has various uses in Xinjiang.
  
  
  OBJECTIVE
  A reversed-phase rapid resolution liquid chromatography (RP-RRLC) method with diode array detector (DAD) was developed for simultaneous determination of diosmin, linarin, and pulegone from Ziziphora clinopodioides Lam, a widely used in traditional Uygur medicine for treating heart disease, high blood pressure, and other diseases.
  
  
  METHODS
  Compounds were separated on a XDB-C18 reversed-phase analytical column (50 mm × 4.6 mm, 1.8 μm) with gradient elution using methanol and 1% aqueous acetic acid (v/v) at 0.9 mL/min. he detection wavelength was set at 270 nm.
  
  
  METHODS
  Ziziphora clinopodioides Lam. were collected from ten different origins in Xinjiang, including the Ban fang ditch, Tuoli, the Altay mountains, Terks, Xiata Road, Zhaosu Highway, Guozigou, Fukang, Jimsar, Wulabo.
  
  
  METHODS
  The intra-day and inter-day precisions of all three compounds were less than 0.89% and the average recoveries ranged from 97.4 to 104.1%. There were highly significant linear correlations between component concentrations and specific chromatographic peak areas (R2 > 0.999).
  
  
  RESULTS
  The proposed method was successfully applied to determine the levels of three active components in Z. clinopodioides Lam. samples from different locations in Xinjiang.
  
  
  CONCLUSIONS
  The proposed method is simple, consistent, accurate, and could be utilized as a quality control method for Z. clinopodioides Lam.
authors.affiliation: Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Xinjiang, China ; Department of Chinese Herbal Medicine, College of TCM, Xinjiang Medical University, Urumqi - 830011, Xinjiang, China.
authors.forename: Shuge
authors.initials: S
authors.lastname: Tian
authors.forename: Qian
authors.initials: Q
authors.lastname: Yu
authors.forename: Dongdong
authors.initials: D
authors.lastname: Wang
authors.forename: Halmuart
authors.initials: H
authors.lastname: Upur
keywords: Diosmin; Ziziphora clinopodioides Lam; linarin; pulegone; reversed-phase-rapid resolution liquid chromatography-diode array detector