title: The detection of inherent homologous recombination between repeat sequences in H. pylori 26695 by the PCR-based method.
abstract: Helicobacter pylori infects more than half of the world's population, making it the most widespread infection of bacteria. It has high genetic diversity and has been considered as one of the most variable bacterial species. In the present study, a PCR-based method was used to detect the presence and the relative frequency of homologous recombination between repeat sequences (>500 bp) in H. pylori 26695. All the recombinant structures have been confirmed by sequencing. The inversion generated between inverted repeats showed distinct features from the recombination for duplication or deletion between direct repeats. Meanwhile, we gave the mathematic reasoning of a general formula for the calculation of relative recombination frequency and indicated the conditions for its application. This formula could be extensively applied to detect the frequency of homologous recombination, site-specific recombination, and other types of predictable recombination. Our results should be helpful for better understanding the genome evolution and adaptation of bacteria.
authors.affiliation: Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Boulevard del Maestro S/N esq. Elías Piña, Col. Narciso Mendoza, Cd., 88710, Reynosa, Tamaulipas, Mexico.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Fu
authors.forename: Reyna Cristina
authors.initials: RC
authors.lastname: Zepeda-Gurrola
authors.forename: Germán Rubén
authors.initials: GR
authors.lastname: Aguilar-Gutiérrez
authors.forename: Edgar E
authors.initials: EE
authors.lastname: Lara-Ramírez
authors.forename: Erick J
authors.initials: EJ
authors.lastname: De Luna-Santillana
authors.forename: Isabel Cristina
authors.initials: IC
authors.lastname: Rodríguez-Luna
authors.forename: Alejandro
authors.initials: A
authors.lastname: Sánchez-Varela
authors.forename: Ricardo
authors.initials: R
authors.lastname: Carreño-López
authors.forename: Víctor Ricardo
authors.initials: VR
authors.lastname: Moreno-Medina
authors.forename: Mario A
authors.initials: MA
authors.lastname: Rodríguez-Pérez
authors.forename: Yolanda
authors.initials: Y
authors.lastname: López-Vidal
authors.forename: Xianwu
authors.initials: X
authors.lastname: Guo
mesh_terms: D004269:DNA, Bacterial; D017353:Gene Deletion; D020440:Gene Duplication; D023061:Gene Order; D016680:Genome, Bacterial; D016480:Helicobacter pylori / Q000235:genetics*; D059765:Homologous Recombination; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004269:DNA, Bacterial