title: TCam-2 seminoma cells exposed to egg-derived microenvironment modify their shape, adhesive pattern and migratory behaviour: a molecular and morphometric analysis.
abstract: Seminoma is one of the most common Testicular Germ Cell Tumours that originates during embryonic development due to an alteration of the local niche that in turn results in a delayed or blocked differentiation of Primordial Germ Cells. The block of differentiation is actually a common way to develop cancer disease as postulated by the "embryonic rest theory of cancer". In agreement with this theory different studies have demonstrated that embryonic cues display the capacity of reprogramming aggressive cancer cells towards a less aggressive phenotype. Herein we investigate the ability of a culture medium added with 10% egg albumen (EW, Egg White) to modulate seminoma cell phenotype and behaviour, by ensuring a proper set of morphogenetic signals. We chose to use the TCam-2 seminoma cell line that has been established as the only available cell line, obtained from a primary testicular seminoma. EW is able to: 1) modify TCam-2 cell spreading rate and cell-substrate adhesion without affecting proliferation and survival indexes; 2) modulate TCam-2 actin distribution pattern increasing cortical localization of actin filaments; 3) increase TCam-2 cell-cell junction capability; 4) decrease both chemo-sensitive and collective TCam-2 migratory behaviour. According to these observations morphometric fractal analysis revealed the ability of EW to increase Circularity and Solidity parameters and, consequently, to decrease Fractal dimension. Prompted by these observations we hypothesize that EW treatment could rescue, at least in part, the neoplastic-metastatic behaviour of seminoma cells.
authors.affiliation: Department of Anatomy, Histology, Forensic Medicine and Orthopedics - Section of Histology and Medical Embryology, Sapienza University of Rome, Rome, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Ferranti
authors.forename: Fabrizio
authors.initials: F
authors.lastname: D'Anselmi
authors.forename: Maria
authors.initials: M
authors.lastname: Caruso
authors.forename: Vittorio
authors.initials: V
authors.lastname: Lei
authors.forename: Simona
authors.initials: S
authors.lastname: Dinicola
authors.forename: Alessia
authors.initials: A
authors.lastname: Pasqualato
authors.forename: Alessandra
authors.initials: A
authors.lastname: Cucina
authors.forename: Alessandro
authors.initials: A
authors.lastname: Palombo
authors.forename: Giulia
authors.initials: G
authors.lastname: Ricci
authors.forename: Angela
authors.initials: A
authors.lastname: Catizone
authors.forename: Mariano
authors.initials: M
authors.lastname: Bizzarri
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D018031:Connexin 43 / Q000378:metabolism; D004527:Egg Proteins / Q000494:pharmacology*; D004529:Egg White; D022001:Focal Adhesions / Q000187:drug effects; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism; D007365:Intercellular Junctions / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D018239:Seminoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D013736:Testicular Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D016596:Vinculin / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: D000199:Actins; D015820:Cadherins; D018031:Connexin 43; D004527:Egg Proteins; D019012:Integrin beta1; D051176:beta Catenin; D016596:Vinculin