title: Production of a monoclonal antibody and development of an immunoassay for detection of Cr(III) in water samples.
abstract: In this study we report the production of a monoclonal antibody (Mab) specific for Cr(III)-chelate and the development of a competitive immunoassay for detection of Cr(III) in water samples. In the assay, the complete antigen (Cr(III)-ITCBE-BSA) was used as coating antigen, and Cr(III)-ITCBE as competitor competes with coating antigen to bind with Mab. Using this approach, the spiked water samples with Cr(III) were detected. The linear range of the detection was 0.7-12.4 ng mL(-1). The limit of the detection (LOD) was 0.51 ng mL(-1). The spiked results were also confirmed by ICP-MS, which showed a good correlation (R(2)=0.997) between the two methods. The results indicated that the developed assay was reliable and suitable for the detection of Cr(III) in water samples.
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China. Electronic address: zhouyu69@sina.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.forename: Y S
authors.initials: YS
authors.lastname: Li
authors.forename: X Y
authors.initials: XY
authors.lastname: Meng
authors.forename: Y Y
authors.initials: YY
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.forename: Z H
authors.initials: ZH
authors.lastname: Li
authors.forename: J H
authors.initials: JH
authors.lastname: Zhang
authors.forename: X R
authors.initials: XR
authors.lastname: Wang
authors.forename: J Q
authors.initials: JQ
authors.lastname: Liu
authors.forename: S Y
authors.initials: SY
authors.lastname: Lu
authors.forename: H L
authors.initials: HL
authors.lastname: Ren
authors.forename: P
authors.initials: P
authors.lastname: Hu
authors.forename: Z S
authors.initials: ZS
authors.lastname: Liu
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002857:Chromium / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D014874:Water Pollutants, Chemical; D002857:Chromium
keywords: Assay; Complete antigen; Cr(III); Mab