title: Amino-functionalized macroporous silica for efficient tryptic digestion in acidic solutions.
abstract: Amino-functionalized macroporous silica foam (NH2 -MOSF) has been developed as a host reactor to realize highly efficient proteolysis in acidic solutions where normal tryptic reactions cannot occur. The digestion protocol consists simply of adding the functionalized NH2 -MOSF into the protein and trypsin solutions without altering the bulk pH or preloading the enzymes on the materials. With this protocol, digestion of sample fractions from LC can be efficiently realized in the acidic solutions directly. Digestion of a protein fraction extracted from rat liver tissue after LC separation was performed to illustrate this principle, where 103 proteins were successfully identified at pH 3 after 1.5 h of tryptic digestion.
authors.affiliation: Department of Chemistry, Institute of Biomedical Sciences and State Key Lab of Molecular Engineering of Polymers, Fudan University, Shanghai, China.
authors.forename: Jinrui
authors.initials: J
authors.lastname: Gan
authors.forename: Kun
authors.initials: K
authors.lastname: Qian
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wan
authors.forename: Liang
authors.initials: L
authors.lastname: Qiao
authors.forename: Weichao
authors.initials: W
authors.lastname: Guo
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
authors.forename: Hubert H
authors.initials: HH
authors.lastname: Girault
authors.forename: Baohong
authors.initials: B
authors.lastname: Liu
mesh_terms: D000818:Animals; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000737:chemistry; D036103:Nanotechnology; D010446:Peptide Fragments / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D010449:Peptide Mapping / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D051381:Rats; D012822:Silicon Dioxide / Q000737:chemistry*; D014357:Trypsin / Q000378:metabolism*
chemical_list: D010446:Peptide Fragments; D012822:Silicon Dioxide; D014357:Trypsin
keywords: Acidic solutions; Amino-functionalized macroporous silica; Mass spectrometry; Nanoproteomics; Peptide mass fingerprinting