title: Construction and targeted retrieval of specific clone from a non-gridded soybean bacterial artificial chromosome library.
abstract: Although a post-genomic era is emerging for many plants, the bacterial artificial chromosome (BAC) library is still a valuable tool for genomic studies and preservation of precious genetic resources. Construction of non-gridded BAC libraries would dramatically reduce cost and save storage space. A non-gridded BAC library composed of approximately 96,000 insert-containing clones in 80 pools with an average insert size of 75 kb was constructed. This library represented 5.2 genome equivalents. We successfully developed a unique procedure to retrieve positive clones from the non-gridded pools. With this retrieving protocol, the non-gridded library system can be adapted to different species and to serve various research needs.
authors.affiliation: Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China; Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. Electronic address: xiazhj@neigaehrb.ac.cn.
authors.forename: Zhengjun
authors.initials: Z
authors.lastname: Xia
authors.forename: Hongyan
authors.initials: H
authors.lastname: Wu
authors.forename: Satoshi
authors.initials: S
authors.lastname: Watanabe
authors.forename: Kyuya
authors.initials: K
authors.lastname: Harada
mesh_terms: D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular; D015723:Gene Library; D013025:Glycine max / Q000235:genetics*
keywords: BAC; Non-gridded library; Pooling; Screening; Soybean