title: Adhesion of tumor cells to matrices and endothelium.
abstract: Adhesion of tumor cells to matrix components and endothelial cells is essential for tumor metastasis. Investigation of the adhesion molecules required and the signals which induce tumor cell adhesion and migration are crucial in order to increase our understanding of this process. This chapter describes protocols which may be used to study tumor cell adhesion to purified matrix elements and tissue sections. It also details methods used to investigate cell adhesion to endothelial cells, both under static and flow conditions. In addition, there is a section detailing the use of endothelial cell cultures on three-dimensional collagen gels which are useful when studying adhesion to endothelial cells and onward invasion through a protein matrix.
authors.affiliation: School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham, UK.
authors.forename: Clara M
authors.initials: CM
authors.lastname: Yates
authors.forename: Helen M
authors.initials: HM
authors.lastname: McGettrick
authors.forename: Gerard B
authors.initials: GB
authors.lastname: Nash
authors.forename: G Ed
authors.initials: GE
authors.lastname: Rainger
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D003094:Collagen / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D004727:Endothelium / Q000187:drug effects / Q000378:metabolism*; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism*; D005782:Gels / Q000494:pharmacology; D006801:Humans; D009369:Neoplasms / Q000473:pathology*; D051381:Rats; D012212:Rheology / Q000187:drug effects
chemical_list: D005782:Gels; D003094:Collagen