title: Mechanical wounding of yeast-like conidium cells of Tremella fuciformis makes them susceptible to Agrobacterium-mediated transformation.
abstract: We developed an efficient protocol that makes possible the practical use of Agrobacterium to transform the yeast-like conidia of Tremella fuciformis. Mechanical wounding of fungal cells prior to co-cultivation with Agrobacterium cells greatly improved transformation efficiency.
authors.affiliation: Department of Biotechnology, Catholic University of Daegu.
authors.forename: Dong-Il
authors.initials: DI
authors.lastname: Shin
authors.forename: Hee-Sung
authors.initials: HS
authors.lastname: Park
mesh_terms: D060054:Agrobacterium / Q000235:genetics*; D001487:Basidiomycota / Q000166:cytology* / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D055595:Mechanical Phenomena; D013172:Spores, Fungal / Q000166:cytology*; D014170:Transformation, Genetic