title: Overexpression, crystallization and preliminary X-ray crystallographic analysis of a putative xylose isomerase from Bacteroides thetaiotaomicron.
abstract: Bacteroides thetaiotaomicron BT0793, a putative xylose isomerase, was overexpressed in Escherichia coli, purified and crystallized using polyethylene glycol monomethyl ether 550 as the precipitant. X-ray diffraction data were collected to 2.10 Å resolution at 100 K using synchrotron X-rays. The crystal was found to belong to space group P1, with unit-cell parameters a=96.3, b=101.7, c=108.3 Å, α=82.8, β=68.2, γ=83.0°. The asymmetric unit contained eight subunits of xylose isomerase with a crystal volume per protein weight (VM) of 2.38 Å3 Da(-1) and a solvent content of 48.3%.
authors.affiliation: Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Jea-Won
authors.initials: JW
authors.lastname: Cho
authors.forename: Byeong-Gu
authors.initials: BG
authors.lastname: Han
authors.forename: Sang Youn
authors.initials: SY
authors.lastname: Park
authors.forename: Seung Jun
authors.initials: SJ
authors.lastname: Kim
authors.forename: Myoung-Dong
authors.initials: MD
authors.lastname: Kim
authors.forename: Byung Il
authors.initials: BI
authors.lastname: Lee
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000737:chemistry*; D001439:Bacteroides / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D007661:Ketoses / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D007661:Ketoses; D011994:Recombinant Proteins; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase
keywords: BT0793; Bacteroides thetaiotaomicron; glucose isomerase; xylose isomerase