title: Evaluation of an in-clinic assay for the diagnosis of canine parvovirus.
abstract: The results of a study designed to evaluate the performance of an in-clinic test for the detection of canine parvovirus (CPV) are reported. A total of 150 faecal samples collected from dogs with acute diarrhoea were tested using the in-clinic test, a haemagglutination assay (HA) and a real-time PCR assay for CPV detection, quantification and characterisation. CPV was detected in 66, 73, and 101 faecal samples by in-clinic, HA and PCR testing, respectively. The relative sensitivity and specificity of the in-clinic test were 86.3% and 96.1%, respectively, when the test was compared to HA, and 65.3% and 100%, respectively, when compared to real-time PCR. The sample distribution according to the virus type was CPV-2a, n=44; CPV-2b, n=11; CPV-2c, n=44, CPV-2, n=2, as determined by minor groove binder probe assays and/or sequence analysis. The percentage of positive in-clinic tests was 70.5% for CPV-2a, 72.7% for CPV-2b and 75.0% for CPV-2c (P>0.05). Using real-time PCR as the reference standard for CPV detection, the in-clinic test was more specific than HA and had comparable sensitivity to HA, demonstrating detection rates similar to those previously observed for other rapid in-clinic tests. The in-clinic test was also able to detect all CPV types at equivalent rates.
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano (Bari), Italy. Electronic address: nicola.decaro@uniba.it.
authors.forename: N
authors.initials: N
authors.lastname: Decaro
authors.forename: C
authors.initials: C
authors.lastname: Desario
authors.forename: M
authors.initials: M
authors.lastname: Billi
authors.forename: E
authors.initials: E
authors.lastname: Lorusso
authors.forename: M L
authors.initials: ML
authors.lastname: Colaianni
authors.forename: V
authors.initials: V
authors.lastname: Colao
authors.forename: G
authors.initials: G
authors.lastname: Elia
authors.forename: G
authors.initials: G
authors.lastname: Ventrella
authors.forename: I
authors.initials: I
authors.lastname: Kusi
authors.forename: S
authors.initials: S
authors.lastname: Bo
authors.forename: C
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000415:Albania; D000818:Animals; D015169:Colony Count, Microbial / Q000379:methods* / Q000662:veterinary; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D003967:Diarrhea / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D004283:Dog Diseases / Q000175:diagnosis* / Q000821:virology; D004285:Dogs; D005243:Feces / Q000821:virology; D006386:Hemagglutination Tests / Q000379:methods* / Q000662:veterinary; D007558:Italy; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods* / Q000662:veterinary; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013030:Spain
chemical_list: D004279:DNA, Viral
keywords: CPV-2c; Canine parvovirus; Diagnosis; In-clinic test