title: Ultrasound-assisted surfactant-enhanced emulsification microextraction for the determination of carbamates in wines by ultra-high performance liquid chromatography-tandem mass spectrometry.
abstract: A new sensitive multiresidue method based on ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) has been developed for the detection, confirmation and quantification of twenty five carbamates in wine samples. The separation was achieved in 5.5 min, using a Zorbax Eclipse plus RRHD C18 column (50 mm×2.1 mm, 1.8 μm), with a mobile phase of water and methanol, both of them with 0.01% formic acid. The analytes were detected in positive mode with multiple reaction monitoring mode. Ultrasound-assisted surfactant-enhanced emulsification microextraction (UASEME), using a low-density extraction solvent has been optimized for the satisfactory extraction of carbamates and clean-up of extracts. The matrix effect was studied, showing that the proposed procedure provides very clean extracts. Under optimum conditions, recoveries for fortified wine samples ranged from 74 to 102%, with relative standard deviations lower than 6%. Limits of quantification ranged from 0.15 to 0.92 μgl(-1), showing the high sensitivity of this fast and simple method and its compliance with current requirements.
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Av. Fuentenueva s/n, 18071 Granada, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Moreno-González
authors.forename: José F
authors.initials: JF
authors.lastname: Huertas-Pérez
authors.forename: Ana M
authors.initials: AM
authors.lastname: García-Campaña
authors.forename: Juan M
authors.initials: JM
authors.lastname: Bosque-Sendra
authors.forename: Laura
authors.initials: L
authors.lastname: Gámiz-Gracia
mesh_terms: D002219:Carbamates / Q000032:analysis*; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004655:Emulsions; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013010:Sonication; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014920:Wine / Q000032:analysis*
chemical_list: D002219:Carbamates; D004655:Emulsions
keywords: Carbamates; UASEME; UHPLC–MS/MS; Wine