title: Prevalence and genetic characterization of Vibrio vulnificus in raw seafood and seawater in Malaysia.
abstract: Vibrio vulnificus is a highly invasive human pathogen that exists naturally in estuarine environment and coastal waters. In this study, we used different PCR assays to detect V. vulnificus in 260 seafood and 80 seawater samples. V. vulnificus was present in about 34 (13%) of the 260 seafood samples and 18 (23%) of the 80 seawater samples. Repetitive extragenic palindromic PCR (REP-PCR) and enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) were applied to subtype the V. vulnificus isolates. Twenty-five REP profiles and 45 ERIC profiles were observed, and the isolates were categorized into 9 and 10 distinct clusters at the similarity of 80%, by REP-PCR and ERIC-PCR, respectively. ERIC-PCR is more discriminative than REP-PCR in subtyping V. vulnificus, demonstrating high genetic diversity among the isolates.
authors.affiliation: Institute of Biological Sciences, Faculty of Science, Laboratory of Biomedical Science and Molecular Microbiology, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Mohammadjavad
authors.initials: M
authors.lastname: Paydar
authors.forename: Kwai Lin
authors.initials: KL
authors.lastname: Thong
mesh_terms: D003257:Consumer Product Safety; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005506:Food Contamination / Q000032:analysis*; D014644:Genetic Variation; D006801:Humans; D008296:Malaysia / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D017747:Seafood / Q000382:microbiology*; D012623:Seawater / Q000382:microbiology*; D041261:Vibrio vulnificus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial