title: Measles virus fusion machinery activated by sialic acid binding globular domain.
abstract: Paramyxoviruses, including the human pathogen measles virus (MV) and the avian Newcastle disease virus (NDV), enter host cells through fusion of the viral envelope with the target cell membrane. This fusion is driven by the concerted action of two viral envelope glycoproteins: the receptor binding protein and the fusion protein (F). The MV receptor binding protein (hemagglutinin [H]) attaches to proteinaceous receptors on host cells, while the receptor binding protein of NDV (hemagglutinin-neuraminidase [HN]) interacts with sialic acid-containing receptors. The receptor-bound HN/H triggers F to undergo conformational changes that render it competent to mediate fusion of the viral and cellular membranes. The mechanism of fusion activation has been proposed to be different for sialic acid-binding viruses and proteinaceous receptor-binding viruses. We report that a chimeric protein containing the NDV HN receptor binding region and the MV H stalk domain can activate MV F to fuse, suggesting that the signal to the stalk of a protein-binding receptor binding molecule can be transmitted from a sialic acid binding domain. By engineering the NDV HN globular domain to interact with a proteinaceous receptor, the fusion activation signal was preserved. Our findings are consistent with a unified mechanism of fusion activation, at least for the Paramyxovirinae subfamily, in which the receptor binding domains of the receptor binding proteins are interchangeable and the stalk determines the specificity of F activation.
authors.affiliation: Departments of Pediatrics.
authors.forename: Aparna
authors.initials: A
authors.lastname: Talekar
authors.forename: Anne
authors.initials: A
authors.lastname: Moscona
authors.forename: Matteo
authors.initials: M
authors.lastname: Porotto
mesh_terms: D000818:Animals; D001717:Birds; D002460:Cell Line; D015707:HN Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006388:Hemagglutinins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008457:Measles / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D008459:Measles virus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D009521:Newcastle Disease / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D009522:Newcastle disease virus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D053586:Virus Internalization
chemical_list: D015707:HN Protein; D006388:Hemagglutinins; D011991:Receptors, Virus; D014760:Viral Fusion Proteins; D014764:Viral Proteins; D019158:N-Acetylneuraminic Acid