title: S-nitroso albumin enhances bone formation in a rabbit calvaria model.
abstract: Nitric oxide (NO) is a mediator involved in bone regeneration. We therefore examined the effect of the novel NO donor, S-nitroso human serum albumin (S-NO-HSA) on bone formation in a rabbit calvaria augmentation model. Circular grooves (8 mm diameter, two per animal) were created by a trephine drill in the cortical bone of 40 rabbits and titanium caps were placed on the rabbit calvaria bone filled with a collagen sponge soaked with either 100 μL S-NO-HSA (5%, 20%) or human albumin (5%, 20%). After 4 weeks the titanium hemispheres were subjected to histological and histomorphometric analysis. Bone formation and the volume of the residual collagen sponge were evaluated. S-NO-HSA treatment groups had a significantly higher volume of newly formed bone underneath the titanium hemispheres compared to the albumin control groups (5%: 15.5 ± 4.0% versus 10.6 ± 2.9%; P < 0.05; 20%: 14.0 ± 4.6% versus 6.0 ± 3.8%; P < 0.01). The volume of residual collagen sponge was also significantly lower in the S-NO-HSA groups compared to the control groups (5%: 0.4 ± 0.5% versus 2.6 ± 2.4%; P < 0.05 and 20%: 1.5 ± 2.7% versus 13.0 ± 18.7%; P < 0.01). This study demonstrates for the first time that S-NO-HSA promotes bone formation by slow NO release. Additionally, S-NO-HSA increases collagen sponge degradation.
authors.affiliation: Department of Oral Surgery, Medical University of Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Fügl
authors.affiliation: Ludwig Boltzmann Cluster for Cardiovascular Research, Medical University of Vienna, Austria.
authors.forename: H
authors.initials: H
authors.lastname: Gasser
authors.affiliation: Department of Oral Surgery, Medical University of Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: G
authors.initials: G
authors.lastname: Watzak
authors.affiliation: Department of Oral Surgery, Medical University of Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Bucher
authors.affiliation: Department of Oral Surgery, Medical University of Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: J
authors.initials: J
authors.lastname: Feierfeil
authors.affiliation: Institute of Physiological Chemistry, Center of Physiological Medicine, Medical University Graz, Austria.
authors.forename: G
authors.initials: G
authors.lastname: Jürgens
authors.affiliation: Department of Oral Surgery, Medical University of Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: G
authors.initials: G
authors.lastname: Watzek
authors.affiliation: Institute of Physiological Chemistry, Center of Physiological Medicine, Medical University Graz, Austria. Electronic address: seth.hallstroem@medunigraz.at.
authors.forename: S
authors.initials: S
authors.lastname: Hallström
authors.affiliation: Department of Oral Surgery, Medical University of Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria; School of Dental Medicine, University of Bern, Switzerland. Electronic address: reinhard.gruber@meduniwien.ac.at.
authors.forename: R
authors.initials: R
authors.lastname: Gruber
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D003094:Collagen / Q000494:pharmacology; D008297:Male; D009603:Nitroso Compounds / Q000494:pharmacology*; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D012886:Skull / Q000187:drug effects* / Q000601:surgery
chemical_list: D009603:Nitroso Compounds; C091665:S-nitrosoalbumin; D012710:Serum Albumin, Bovine; D003094:Collagen
keywords: augmentation; bone regeneration; guided bone regeneration; nitric oxide; rabbit