title: miR-106a confers cisplatin resistance by regulating PTEN/Akt pathway in gastric cancer cells.
abstract: Recent studies have shown that microRNA-106a (miR-106a) is overexpressed in gastric cancer and contributes to tumor growth. In this study, we investigated whether miR-106a mediated resistance of the gastric cancer cell line SGC7901 to the chemotherapeutic agent cisplatin (DDP). MiR-106a expression was up-regulated in the DDP resistant cell line SGC7901/DDP compared with its parental line SGC7901. Transfection of miR-106a induced DDP resistance in SGC7901, while suppression of miR-106a in SGC7901/DDP led to enhanced DDP cytotoxicity. Further study indicated that the mechanism of miR-106a-induced DDP resistance involved the expression of phosphatase and tensin homolog deleted from chromosome 10 (PTEN) protein and its downstream phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) pathway. This study provides a novel mechanism of DDP resistance in gastric cancer.
authors.affiliation: Department of Central Laboratory, The Affiliated People's Hospital, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Fang
authors.forename: Huiling
authors.initials: H
authors.lastname: Shen
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cao
authors.forename: Rong
authors.initials: R
authors.lastname: Qin
authors.forename: Lulu
authors.initials: L
authors.lastname: Long
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Zhu
authors.forename: Chanjuan
authors.initials: C
authors.lastname: Xie
authors.forename: Wenlin
authors.initials: W
authors.lastname: Xu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D001483:Base Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D017931:DNA Primers; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D015854:Up-Regulation
chemical_list: D000970:Antineoplastic Agents; D017931:DNA Primers; C542541:MIRN106 microRNA, human; D035683:MicroRNAs; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human; D002945:Cisplatin
keywords: PTEN; gastric cancer; miR-106a