title: Lactobacillus plantarum HY7712 protects against the impairment of NK-cell activity caused by whole-body γ-irradiation in mice.
abstract: While searching for lactic acid bacteria that can restore aging-impaired immune responses, we isolated the Toll-like receptor (TLR) 2/NF-kappaB-activating strain Lactobacillus plantarum HY7712 from kimchi and investigated its immunomodulating effect in whole-body γ-irradiated mice. Exposure to HY7712 strongly activated NF-kappaB signaling in RAW264.7 cells, but inhibited lipopolysaccharide-stimulated NF-kappaB activation. Moreover, HY7712 protected against the downregulation of interferon (IFN)-γ and upregulation of interleukin (IL)-13 caused by γ-irradiation in mice. In mice, γ-irradiation impaired NK-cell activity against YAC-1 tumor cells, but following HY7712 exposure, the activity of NK cells was restored to 91.5% of the level measured in control mice (p < 0.05). These findings suggest that HY7712 activates the TLR2/NF-kappaB signaling pathway and protects against the impairment of NK-cell activity caused by γ-irradiation or aging.
authors.affiliation: College of Pharmacy, Kyung Hee University, Seoul 130-702, Republic of Korea.
authors.forename: Hoyong
authors.initials: H
authors.lastname: Lee
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Ahn
authors.forename: Se-Hoon
authors.initials: SH
authors.lastname: Park
authors.forename: Do-Young
authors.initials: DY
authors.lastname: Park
authors.forename: Young-Woo
authors.initials: YW
authors.lastname: Jin
authors.forename: Cha Soon
authors.initials: CS
authors.lastname: Kim
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Sung
authors.forename: Chul-Sung
authors.initials: CS
authors.lastname: Huh
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
mesh_terms: D000818:Animals; D002460:Cell Line; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D018793:Interleukin-13 / Q000276:immunology / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology* / Q000528:radiation effects*; D048191:Lactiplantibacillus plantarum / Q000254:growth & development* / Q000276:immunology*; D008264:Macrophages / Q000276:immunology / Q000382:microbiology; D051379:Mice; D016328:NF-kappa B / Q000276:immunology / Q000378:metabolism; D015398:Signal Transduction; D051195:Toll-Like Receptor 2 / Q000276:immunology / Q000378:metabolism; D014916:Whole-Body Irradiation
chemical_list: D018793:Interleukin-13; D016328:NF-kappa B; C493486:Tlr2 protein, mouse; D051195:Toll-Like Receptor 2; D007371:Interferon-gamma