title: Effect of glycerol on GHR and IGF-1 gene expression in breast muscle and on growth of Japanese meat quails.
abstract: We evaluated messenger RNA (mRNA) expression of the growth-hormone (GHR) and insulin-like growth factor (IGF-1) genes in 28-day-old Japanese meat quails fed diets containing 0, 8, or 12% dietary glycerol in substitution of corn. Total RNA was extracted from the breast muscle and the DNA was amplified with specific primers using real-time PCR. Feed conversion ratio and feed intake were evaluated. The birds fed 8 and 12% glycerol presented higher IGF-1 mRNA expression [0.059 and 0.049 arbitrary units (AU), respectively] relative to those not fed with glycerol (0.029 AU), while 12% glycerol reduced GHR mRNA expression (0.022 AU). Dietary inclusion of 8% glycerol promoted similar performance results (feed conversion) as the diet with no glycerol. We conclude that inclusion of glycerol in the diet affects GHR and IGF-1 gene expression in Japanese meat quails. However, considering the performance results and the expression of the GHR and IGF-1 genes, 8% glycerol may be safely included in the diet of meat quails.
authors.affiliation: Programa de Pós-Graduação em Zootecnia, Universidade Estadual de Maringá stefania_caroline@hotmail.com.
authors.forename: S C C
authors.initials: SC
authors.lastname: Silva
authors.forename: E
authors.initials: E
authors.lastname: Gasparino
authors.forename: D M
authors.initials: DM
authors.lastname: Voltolini
authors.forename: A V
authors.initials: AV
authors.lastname: Pires
authors.forename: F
authors.initials: F
authors.lastname: Tanamati
authors.forename: A L S
authors.initials: AL
authors.lastname: Garcia
authors.forename: D P
authors.initials: DP
authors.lastname: de Oliveira
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D003370:Coturnix; D004032:Diet / Q000662:veterinary; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression; D005990:Glycerol / Q000494:pharmacology*; D013006:Growth Hormone / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D008460:Meat / Q000032:analysis*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D005990:Glycerol