title: Water miscible mono alcohols' effect on the proteolytic performance of Bacillus clausii serine alkaline protease.
abstract: In this study, our investigations showed that the increasing concentrations of all examined mono alcohols caused a decrease in the Vm, kcat and kcat/Km values of Bacillus clausii GMBE 42 serine alkaline protease for casein hydrolysis. However, the Km value of the enzyme remained almost the same, which was an indicator of non-competitive inhibition. Whereas inhibition by methanol was partial non-competitive, inhibition by the rest of the alcohols tested was simple non-competitive. The inhibition constants (KI) were in the range of 1.32-3.10 M, and the order of the inhibitory effect was 1-propanol>2-propanol>methanol>ethanol. The ΔG(≠) and ΔG(≠)E-T values of the enzyme increased at increasing concentrations of all alcohols examined, but the ΔG(≠)ES value of the enzyme remained almost the same. The constant Km and ΔG(≠)ES values in the presence and absence of mono alcohols indicated the existence of different binding sites for mono alcohols and casein on enzyme the molecule. The kcat of the enzyme decreased linearly by increasing log P and decreasing dielectric constant (D) values, but the ΔG(≠) and ΔG(≠)E-T values of the enzyme increased by increasing log P and decreasing D values of the reaction medium containing mono alcohols.
authors.affiliation: Faculty of Arts and Sciences, Department of Chemistry, Section of Biochemistry, Kocaeli University, Umuttepe Campus, 41380, İzmit-Kocaeli, Turkey.
authors.forename: Yonca Avci
authors.initials: YA
authors.lastname: Duman
authors.forename: Dilek
authors.initials: D
authors.lastname: Kazan
authors.forename: Aziz Akin
authors.initials: AA
authors.lastname: Denizci
authors.forename: Altan
authors.initials: A
authors.lastname: Erarslan
mesh_terms: D000438:Alcohols / Q000737:chemistry* / Q000494:pharmacology*; D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D010450:Endopeptidases / Q000378:metabolism*; D006868:Hydrolysis / Q000187:drug effects; D007700:Kinetics; D011480:Protease Inhibitors / Q000737:chemistry / Q000494:pharmacology; D059748:Proteolysis / Q000187:drug effects*; D013816:Thermodynamics; D014867:Water / Q000737:chemistry*
chemical_list: D000438:Alcohols; D001426:Bacterial Proteins; D011480:Protease Inhibitors; D014867:Water; D010450:Endopeptidases; C087822:alkaline protease