title: The association of interleukin-16 gene polymorphisms with IL-16 serum levels and risk of nasopharyngeal carcinoma in a Chinese population.
abstract: Interleukin (IL)-16 plays a fundamental role in inflammatory diseases, as well as in the development and progression of tumors. Genetic variation in DNA sequence of IL16 gene may lead to altered cytokine production and/or activity, and this variation may modulate an individual's susceptibility to nasopharyngeal carcinoma (NPC). To test this hypothesis, we investigated the association of IL16 gene polymorphisms and serum IL-16 levels with NPC risk in a Chinese population. We analyzed IL16 gene rs11556218 T/G, rs4778889 T/C, and rs4072111 C/T polymorphisms using PCR-RFLP and DNA sequencing, and serum IL-16 levels were measured by ELISA. The IL16 rs11556218 T/G polymorphism was significantly associated with the susceptibility to NPC patients. The TG genotype was associated with a significantly higher risk of NPC as compared with the TT genotype (OR = 2.05, 95% CI 1.04-4.01; p = 0.037). Patients carrying the G allele had a significantly higher risk for developing NPC compared with individuals carrying the T allele (OR = 1.79, 95% CI 1.07-3.01; p = 0.027). The serum IL-16 levels were increased in NPC patients compared with controls (p < 0.01); the genotypes carrying the IL16 rs11556218 G variant allele were associated with increased serum IL-16 levels compared with the homozygous wild-type genotype in NPC patients (all p values <0.01). Our data suggested that IL16 rs11556218 T/G polymorphism was associated with increased susceptibility to NPC through increasing the production of serum IL-16 levels.
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Qin
authors.forename: Qiliu
authors.initials: Q
authors.lastname: Peng
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Lao
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Chen
authors.forename: Yu
authors.initials: Y
authors.lastname: Lu
authors.forename: Xianjun
authors.initials: X
authors.lastname: Lao
authors.forename: Cuiju
authors.initials: C
authors.lastname: Mo
authors.forename: Jingzhe
authors.initials: J
authors.lastname: Sui
authors.forename: Junrong
authors.initials: J
authors.lastname: Wu
authors.forename: Limin
authors.initials: L
authors.lastname: Zhai
authors.forename: Shi
authors.initials: S
authors.lastname: Yang
authors.forename: Shan
authors.initials: S
authors.lastname: Li
authors.forename: Jinmin
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics*; D002277:Carcinoma; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D019410:Interleukin-16 / Q000097:blood* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000097:blood* / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors
chemical_list: D019410:Interleukin-16