title: Polycarbazole-based organic photodiodes for highly sensitive chemiluminescent immunoassays.
abstract: It is reported the development of a polycarbazole-based organic photodetector for chemiluminescent immunoassays. The optical detector comprised a 1∶4 blend by weight of poly [N-9'-heptadecanyl-2,7-carbazole-alt-5,5-(4',7'-di-2-thienyl-2',1',3'-benzothiadiazole)] (PCDTBT) and [6,6]-phenyl C71-butyric acid methyl ester (PC70BM). Optimization of the photodetector design was conducted aiming to maximize photosensitivity and reduce the background level. Quantitation of recombinant human thyroid stimulating hormone indicated good linearity and yielded a detection sensitivity of ∼3.7 nA × nM(-1) and a detection limit of 80 pg/ml.
authors.forename: Nuno M M
authors.initials: NM
authors.lastname: Pires
authors.forename: Tao
authors.initials: T
authors.lastname: Dong
mesh_terms: D015374:Biosensing Techniques; D002227:Carbazoles / Q000737:chemistry*; D004560:Electricity; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods*; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods*; D055095:Optics and Photonics / Q000295:instrumentation*; D009930:Organic Chemicals / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011994:Recombinant Proteins / Q000032:analysis; D012815:Signal Processing, Computer-Assisted; D019809:Streptavidin / Q000378:metabolism; D013457:Sulfur Compounds / Q000737:chemistry*; D013830:Thiadiazoles / Q000737:chemistry; D013972:Thyrotropin / Q000032:analysis
chemical_list: D002227:Carbazoles; D009930:Organic Chemicals; D011108:Polymers; D011994:Recombinant Proteins; D013457:Sulfur Compounds; D013830:Thiadiazoles; C000600521:poly(N-9'-heptadecanyl-2,7-carbazole-alt-5,5-(4',7'-di-2-thienyl-2',1',3'-benzothiadiazole); C041514:carbazole; C015700:benzo-1,2,3-thiadiazole; D013972:Thyrotropin; D019809:Streptavidin; D006735:Horseradish Peroxidase