title: [MicroRNA-10a accelerates endodermal lineage differentiation of mesenchymal stem cells from human placenta].
abstract: OBJECTIVE
  To improve the potential of endodermal differentiation of human placenta-derived mesenchymal stem cells (PMSCs) by microRNA-10a (miR-10a)-mediated post-transcriptional regulation of its mRNA targets.
  
  
  METHODS
  Lentiviral vectors were used to stably and specifically over-express miR-10a and inhibited the miR-10a function by its antagomir. In addition, the relationship between miR-10a and Hoxa1 expression was analyzed. Real-time quantitative PCR (qRT-PCR) and immunofluorescent cytochemical staining were utilized to test the mRNA and protein expression variation and assess the ability of PMSCs to differentiate into endodermal cells. Results Over-expression of miR-10a led to the suppression of endogenous Hoxa1 expression, and inhibition of miR-10a relieved the repression of Hoxa1. Over-expression of miR-10a in PMSCs resulted in the up-regulation of endoderm-specific genes (FoxA2, Sox-17, Pdx-1 and Cdx2) and the increased proportions of FOXA2, SOX-17 and PDX-1 positive events as compared with the control treated cells.
  
  
  CONCLUSIONS
  miR-10a was up-regulated during endodermal differentiation of PMSCs and involved in its differentiation partially via the suppression of the Hoxa1 gene. Furthermore, the miR-10a accelerates endodermal differentiation, likely mediated by the up-reguation of endoderm-specific down-stream genes.
authors.affiliation: Institute of Human Stem Cell, General Hospital, Key Laboratory of Fertility Preservation and Maintenance, Ministry of Education, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Chen
authors.forename: Haibin
authors.initials: H
authors.lastname: Ma
authors.forename: Shudan
authors.initials: S
authors.lastname: Liu
authors.forename: Libin
authors.initials: L
authors.lastname: Wang
authors.forename: Yukui
authors.initials: Y
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Wei
mesh_terms: D002454:Cell Differentiation; D019070:Cell Lineage; D004707:Endoderm / Q000166:cytology*; D005260:Female; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D035683:MicroRNAs / Q000502:physiology*; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D014157:Transcription Factors / Q000235:genetics; D000099261:Homeobox A1 Protein
chemical_list: D018398:Homeodomain Proteins; D035683:MicroRNAs; D014157:Transcription Factors; D000099261:Homeobox A1 Protein; C547183:MIRN10 microRNA, human