title: Sulfitobacter porphyrae sp. nov., isolated from the red alga Porphyra yezoensis.
abstract: Gram-stain-negative, aerobic, halophilic bacteria, designated SCM-1(T), LCM10-1 and CTBL-B-147, were isolated from modified half-strength SWM-III medium, PES medium and thalli after laboratory cultivation of a red alga, Porphyra yezoensis. Phylogenetic analysis of 16S rRNA gene sequences indicated that the new isolates were affiliated to the genus Sulfitobacter of the class Alphaproteobacteria, and the 16S rRNA gene sequence similarity of the new isolates with the closest related species, Sulfitobacter mediterraneus CH-B427(T), was 98.8%. The DNA G+C contents of the new isolates were in the range of 61.4-62.3 mol%. DNA-DNA relatedness values of strain SCM-1(T) with other type strains of the genus Sulfitobacter were less than 15.9%. The new isolates contained Q-10 as the predominant ubiquinone, phosphatidylcholine, phosphatidylglycerol, an unidentified amino lipid and an unidentified lipid as the main polar lipids, and C(18 : 1)ω7c, C(19 : 1)ω7c and C(16 : 0) as the major fatty acids (>10% of the total). Strain SCM-1(T) could be differentiated from Sulfitobacter mediterraneus JCM 21792(T) by 35 morphological and phenotypic characteristics. On the basis of the phylogenetic, genetic and phenotypic properties of the new isolates, the name Sulfitobacter porphyrae sp. nov. is proposed, with strain SCM-1(T) ( = LMG 27110(T) = NBRC 109054(T)) as the type strain.
authors.affiliation: National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama 236-8648, Japan.
authors.forename: Youhei
authors.initials: Y
authors.lastname: Fukui
authors.affiliation: National Fisheries University, Shimonoseki 759-6595, Japan.
authors.forename: Mahiko
authors.initials: M
authors.lastname: Abe
authors.affiliation: Seikai National Fisheries Research Institute, Fisheries Research Agency, Nagasaki 851-2213, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kobayashi
authors.affiliation: Tokyo Bay Fisheries Laboratory, Chiba Prefectural Fisheries Research Center, Futtsu, Chiba 293-0042, Japan.
authors.forename: Yushi
authors.initials: Y
authors.lastname: Shimada
authors.affiliation: National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama 236-8648, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Saito
authors.affiliation: National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, Hatsukaichi, Hiroshima 739-0452, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Oikawa
authors.affiliation: Hokkaido National Fisheries Research Institute, Fisheries Research Agency, Sapporo 062-0922, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yano
authors.affiliation: National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama 236-8648, Japan.
authors.forename: Masataka
authors.initials: M
authors.lastname: Satomi
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010713:Phosphatidylcholines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D010802:Phylogeny; D044664:Porphyra / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2