title: Membrane surface functionalization via theophylline derivative coating and streptavidin immobilization.
abstract: Poly(vinylidene fluoride) (PVDF) and regenerated cellulose (RC) membranes were surface-modified by the adsorption of one adenosine receptor antagonist: the theophylline-oligo(ethylene glycol)-alkene derivative, Theo1. Surface modification was carried out by immersion of the membrane in a dichloromethane solution of Theo1 (PVDF+Theo1 and RC+Theo1 samples). Membrane surfaces with partial coverage by theophylline and/or its inclusion in the membrane structures were studied by X-ray photoelectron spectroscopy (XPS), solid-state nuclear magnetic resonance (SNMR), impedance spectroscopy (IS) and contact angle (CA) measurements. The Theo1 orientation was inferred from the data. Streptavidin (SA) was immobilized onto the membrane/Theo1 hybrid material. The protein-theophylline Theo1 interaction was visualized with bright field microscopy (BFM).
authors.affiliation: Dep. de Química Orgánica, Facultad de Ciencias, Universidad de Málaga, E-29071 Málaga, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Hierrezuelo
authors.affiliation: Dep. de Física Aplicada I, Facultad de Ciencias, Universidad de Málaga, E-29071 Málaga, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Romero
authors.affiliation: Dep. de Física Aplicada I, Facultad de Ciencias, Universidad de Málaga, E-29071 Málaga, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Benavente
authors.affiliation: Dep. de Química Orgánica, Facultad de Ciencias, Universidad de Málaga, E-29071 Málaga, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Rico
authors.affiliation: Dep. de Química Orgánica, Facultad de Ciencias, Universidad de Málaga, E-29071 Málaga, Spain. Electronic address: jmromero@uma.es.
authors.forename: J Manuel
authors.initials: JM
authors.lastname: López-Romero
mesh_terms: D008567:Membranes, Artificial; D008853:Microscopy; D011485:Protein Binding; D019809:Streptavidin / Q000737:chemistry*; D013806:Theophylline / Q000737:chemistry*
chemical_list: D008567:Membranes, Artificial; D019809:Streptavidin; D013806:Theophylline
keywords: Biosensor; Membrane modification; Physicochemical characterization; Theophylline