title: Physiological quality and gene expression related to heat-resistant proteins at different stages of development of maize seeds.
abstract: We quantified and characterized the expression of heat-resistant proteins during seed development of maize lines with distinct levels of tolerance to high drying temperature. A corn field was planted for multiplication of seeds of different lines, two tolerant and two non-tolerant to high drying temperatures. Harvest of the seeds was carried out at various stages of development and they were then subjected to tests of moisture content, germination, first count of germination, accelerated aging, and cold test. The seeds were stored in a freezer for later analysis of expression of heat-resistant proteins by means of real-time PCR, electrophoresis, and spectrophotometry. We observed that heat-resistant proteins are expressed in a differential manner in seeds from different lines and at different stages of development. The expression of heat-resistant proteins was earlier in lines tolerant to high drying temperatures. Greater germination and vigor values was found for seeds collected at the last stage of development.
authors.affiliation: Departamento de Agricultura, Universidade Federal de Lavras, Lavras, MG, Brasil thaisandradeagro@gmail.com.
authors.forename: T
authors.initials: T
authors.lastname: Andrade
authors.forename: E V R
authors.initials: EV
authors.lastname: Von Pinho
authors.forename: R G
authors.initials: RG
authors.lastname: Von Pinho
authors.forename: G E
authors.initials: GE
authors.lastname: Oliveira
authors.forename: V
authors.initials: V
authors.lastname: Andrade
authors.forename: J S
authors.initials: JS
authors.lastname: Fernandes
mesh_terms: D018744:DNA, Plant / Q000235:genetics; D004586:Electrophoresis; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000235:genetics; D006358:Hot Temperature; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012639:Seeds / Q000235:genetics* / Q000502:physiology; D017422:Sequence Analysis, DNA; D013053:Spectrophotometry; D003313:Zea mays / Q000235:genetics* / Q000502:physiology
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins