title: A simple high-performance liquid chromatographic method for the determination of brazilin and its application to a pharmacokinetic study in rats.
abstract: BACKGROUND
  Caesalpinia sappan is a medicinal plant native to China popularly used to treat chronic pelvic inflammation, dysmenorrhea and hysteromyoma. Its main bioactive component is brazilin which had presented antibacterial, anti-inflammatory and anti-platelet aggregation activities. To establish a sensitive, selective, reproducible, and accurate high performance liquid chromatographic (HPLC) method for the quantitative determination of brazilin in plasma, and study the pharmacokinetics of brazilin in rats after intravenous administration of brazilin.
  
  
  METHODS
  Rats received intravenous injection of 25, 50 and 100mg/kg of brazilin. Concentrations of brazilin in plasma were determined by HPLC method at different time points and all pharmacokinetic parameters were estimated by non-compartmental analysis with WinNonLin 6.2 software.
  
  
  RESULTS
  After single intravenous doses of 25, 50 and 100mg/kg brazilin in rats, the main PK parameters were as follows: Cmax were 18.1 ± 4.1, 46.7 ± 8.7 and 82.2 ± 9.6 µg/mL; AUC0-24 were 20.4 ± 4.3, 48.7 ± 6.8 and 90.4 ± 10.3 µgh/mL; and t1/2 were 5.4 ± 1.5, 5.8 ± 0.9 and 6.2 ± 1.2h, respectively.
  
  
  CONCLUSIONS
  It showed that the brazilin was eliminated moderately in rat by intravenous injection route with t1/2 of 6h and showed a dose-dependence profile of Cmax and AUC0-24 at the doses of 25~100mg/kg of brazilin for injection in rats.
authors.affiliation: Department of Pharmacy, First Affiliated Hospital of the Fourth Military Medical University, Changle West Street 127, Xi'an, Shaanxi 710032, China. Electronic address: 18691555435@163.com.
authors.forename: Jia
authors.initials: J
authors.lastname: Yan-yan
authors.affiliation: Department of Pharmacy, First Affiliated Hospital of the Fourth Military Medical University, Changle West Street 127, Xi'an, Shaanxi 710032, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.affiliation: Department of Pharmacy, First Affiliated Hospital of the Fourth Military Medical University, Changle West Street 127, Xi'an, Shaanxi 710032, China.
authors.forename: Song
authors.initials: S
authors.lastname: Ying
authors.affiliation: Department of Pharmacy, First Affiliated Hospital of the Fourth Military Medical University, Changle West Street 127, Xi'an, Shaanxi 710032, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Jinyi
authors.affiliation: Department of Pharmacy, First Affiliated Hospital of the Fourth Military Medical University, Changle West Street 127, Xi'an, Shaanxi 710032, China.
authors.forename: Dou
authors.initials: D
authors.lastname: Fang
authors.affiliation: Department of Pharmacy, First Affiliated Hospital of the Fourth Military Medical University, Changle West Street 127, Xi'an, Shaanxi 710032, China.
authors.forename: Sun
authors.initials: S
authors.lastname: Yuan
authors.affiliation: Department of Pharmacy, First Affiliated Hospital of the Fourth Military Medical University, Changle West Street 127, Xi'an, Shaanxi 710032, China. Electronic address: adwen-2004@hotmail.com.
authors.forename: Wen
authors.initials: W
authors.lastname: Ai-dong
mesh_terms: D000818:Animals; D019540:Area Under Curve; D001578:Benzopyrans / Q000097:blood / Q000737:chemistry* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D006207:Half-Life; D008297:Male; D015394:Molecular Structure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D014011:Tinidazole / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D001578:Benzopyrans; D014011:Tinidazole; C044362:brazilin
keywords: Brazilin; Caesalpinia sappan; HPLC; Pharmacokinetic; Rat