title: [Role of bone marrow-derived mesenchymal stem cells in treating colitis through Fas/FasL-mediated immune regulation].
abstract: OBJECTIVE
  To investigate the difference in the therapeutic effects of bone marrow-derived mesenchymal stem cells (BMSCs) of mice from ovariectomy (OVX) group and sham group in treating colitis, and then further study the differences of Fas/FasL expression and downstream T cell migration and apoptosis between the two groups.
  
  
  METHODS
  The osteoporosis animal models were set up by ovariectomy in C57BL6 mice. Meanwhile, 3% dextran sulfate sodium (DSS) was administered for inducing colitis. We compared the therapeutic effects of BMSCs from OVX and sham groups in treating colitis, in addition, detected the expression of Fas/FasL in BMSCsby means of RT-PCR and Western blotting. The ability of BMSCs from the two groups of inducing T cell migaration and apoptosis was also detected.
  
  
  RESULTS
  Compared with the sham group, BMSCs from OVX mice expressed a lower level of Fas/FasL and displayed a decreased ability of inducing T cell migration and apoptosis, thus leading to an inferior therapeutic effect in treating colitis in animal models.
  
  
  CONCLUSIONS
  Fas/FasL expression of BMSCs from the OVX mice is down regulated, thus leading to a decrease of the migration and apoptosis for T cells from mouse colitis.
authors.affiliation: Department of Endodontics, Affliated Hospital of Stomatology, Chongqing Medical University, Chongqing 400015, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Bingyi
authors.initials: B
authors.lastname: Shao
authors.forename: Yi
authors.initials: Y
authors.lastname: Shuai
authors.forename: Fengqing
authors.initials: F
authors.lastname: Shang
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.forename: Zhifei
authors.initials: Z
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: Liao
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.forename: Deqin
authors.initials: D
authors.lastname: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis; D002465:Cell Movement; D003092:Colitis / Q000276:immunology / Q000628:therapy*; D053222:Fas Ligand Protein / Q000502:physiology*; D005260:Female; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology; D019014:fas Receptor / Q000502:physiology*
chemical_list: D053222:Fas Ligand Protein; D019014:fas Receptor