title: Preparation and characterization of nanoliposomes entrapping medium-chain fatty acids and vitamin C by lyophilization.
abstract: The complex nanoliposomes encapsulating both a hydrophilic drug vitamin C (vit C) and hydrophobic drug medium-chain fatty acids (MCFAs) was prepared by combining double emulsion method with dynamic high pressure microfluidization. The complex nanoliposomes was further freeze-dried under -86 °C for 48 h with sucrose at the sucrose/lipids ratio of 2:1(w/w) in order to enhance its stability. The freeze-dried complex nanoliposomes under the suitable conditions exhibited high entrapment efficiency of MCFAs (44.26 ± 3.34)%, relatively high entrapment efficiency of vit C (62.25 ± 3.43)%, low average size diameter (110.4 ± 7.28) nm and good storage stability at 4 °C for 60 days with slight changes in mean particle diameter and drug entrapment efficiencies. The results of transmission electron microscopy of freeze-dried complex nanoliposomes also showed that the freeze-dried samples with sucrose were stable without great increase in their particle sizes and without destroying their spherical shape. The results indicated that sucrose presented well protection effects in MCFAs-vit C complex nanoliposomes, suggesting the possibility of further usage in commercial liposomes.
authors.affiliation: Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China. yqhu@zju.edu.cn.
authors.forename: Shuibing
authors.initials: S
authors.lastname: Yang
authors.forename: Chengmei
authors.initials: C
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Haixia
authors.initials: H
authors.lastname: Yu
authors.forename: Huijuan
authors.initials: H
authors.lastname: Zheng
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Hu
mesh_terms: D001205:Ascorbic Acid / Q000737:chemistry*; D004356:Drug Storage; D005227:Fatty Acids / Q000737:chemistry*; D005612:Freeze Drying / Q000379:methods; D008081:Liposomes / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size
chemical_list: D005227:Fatty Acids; D008081:Liposomes; D001205:Ascorbic Acid