title: Anti-anaemia efficacy of β-lactoglobulin hydrolysate-iron complex on iron-deficient anaemic rats.
abstract: OBJECTIVE
  The effects of orally administered β-lactoglobulin hydrolysate-iron complex (β-LGH-Fe) on haematological and biochemical parameters in anaemic rats were evaluated. Female weaning Sprague-Dawley rats were fed with iron-deficient diet to induce iron deficiency anaemia. After 6 weeks, the obtained anaemic rats were divided into five groups: iron deficiency control group (iron-deficient diet without β-LGH-Fe complex supplementation, IDC); three groups supplemented with different dosages of β-LGH-Fe complex (0.5 mg Fe/kg BW, iron-deficient diet with low β-LGH-Fe, IDLFe; 2.0 mg Fe/kg BW, iron-deficient diet with medium β-LGH-Fe, IDMF; 4.0 mg Fe/kg BW, iron-deficient diet with high β-LGH-Fe, IDHFe); and ferrous sulphate-supplemented group at a dosage of 2.0 mg Fe/kg BW.
  
  
  RESULTS
  β-LGH-Fe complex could significantly improve hematocrit and haemoglobin decrease, and normalise the serum iron level, total iron-binding capacity and transferrin saturation of anaemic rats in a dose-dependent manner. Serum ferritin content and hepatic nonheme iron level were also increased. In addition, the antioxidant enzyme activities of superoxidase dismutase, catalase and glutathione peroxidase in both plasma and liver homogenate were improved. The production of malondialdehyde and pro-inflammatory cytokines (TNF-α and IL-6) decreased.
  
  
  CONCLUSIONS
  It suggests that β-LGH-Fe complex can ameliorate iron deficiency anaemia, which might make it a potential ingredient with anti-anaemia activity.
authors.affiliation: Key Laboratory of Functional Dairy, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Mao
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Ting
authors.initials: T
authors.lastname: Ai
authors.forename: Jing-Jiao
authors.initials: JJ
authors.lastname: Ma
authors.forename: Ying-Hui
authors.initials: YH
authors.lastname: Li
mesh_terms: D018798:Anemia, Iron-Deficiency / Q000097:blood / Q000178:diet therapy* / Q000378:metabolism; D000818:Animals; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000097:blood / Q000378:metabolism; D019587:Dietary Supplements; D005260:Female; D005293:Ferritins / Q000819:agonists / Q000097:blood; D006397:Hematinics / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D006454:Hemoglobins / Q000819:agonists / Q000032:analysis; D007501:Iron / Q000032:analysis; D007502:Iron Chelating Agents / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D019266:Iron, Dietary / Q000008:administration & dosage / Q000627:therapeutic use*; D007782:Lactoglobulins / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D008099:Liver / Q000737:chemistry / Q000201:enzymology; D008315:Malondialdehyde / Q000037:antagonists & inhibitors / Q000097:blood / Q000378:metabolism; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000097:blood / Q000378:metabolism; D011492:Protein Hydrolysates / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D014886:Weaning
chemical_list: D016207:Cytokines; D006397:Hematinics; D006454:Hemoglobins; D007502:Iron Chelating Agents; D019266:Iron, Dietary; D007782:Lactoglobulins; D011492:Protein Hydrolysates; D008315:Malondialdehyde; D005293:Ferritins; D007501:Iron; D010088:Oxidoreductases