title: Toward one-pot lipase-catalyzed synthesis of poly(ε-caprolactone) particles in aqueous dispersion.
abstract: The preparation of polyester particles using enzyme-catalyzed (lipase from Candida antarctica B, CALB) ring-opening polymerization of ε-caprolactone (ε-CL) in aqueous dispersion was demonstrated for the first time. Immobilization of CALB enabled a significant increase of the number-average degree of polymerization of ε-CL oligomers (up to 38) as compared to dissolved CALB (8 at the maximum). The nature and amount of lipase, as well as the nature of the support material were identified as key parameters controlling ring-opening polymerization of ε-CL in aqueous dispersion. In addition, the involvement of solubilized monomers in polymerization elementary reactions was demonstrated and the consequences on oligomers average length were detailed. An overall mechanism of lipase-catalyzed ε-CL polymerization in aqueous dispersion taking into account the colloidal nature of reaction medium was proposed on the basis of experimental results.
authors.affiliation: Department of Biotechnology, Faculty of Science, Mahidol University, Rama VI Road, Phayathai, Bangkok 10400, Thailand; Project Group BioCat, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Schulgasse 11a, 94315 Straubing, Germany. Electronic address: pranee.inprakhon@igb.fraunhofer.de.
authors.forename: Pranee
authors.initials: P
authors.lastname: Inprakhon
authors.affiliation: Department of Biotechnology, Faculty of Science, Mahidol University, Rama VI Road, Phayathai, Bangkok 10400, Thailand.
authors.forename: Pabhangkon
authors.initials: P
authors.lastname: Panlawan
authors.affiliation: Department of Biotechnology, Faculty of Science, Mahidol University, Rama VI Road, Phayathai, Bangkok 10400, Thailand.
authors.forename: Thunyarat
authors.initials: T
authors.lastname: Pongtharankul
authors.affiliation: CNRS, LCPM, FRE 3564, Nancy, F-54001, France; Université de Lorraine, LCPM, FRE 3564, Nancy, F-54001, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Marie
authors.affiliation: Project Group BioCat, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Schulgasse 11a, 94315 Straubing, Germany.
authors.forename: Lars O
authors.initials: LO
authors.lastname: Wiemann
authors.affiliation: CNRS, LCPM, FRE 3564, Nancy, F-54001, France; Université de Lorraine, LCPM, FRE 3564, Nancy, F-54001, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Durand
authors.affiliation: Project Group BioCat, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Schulgasse 11a, 94315 Straubing, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Sieber
mesh_terms: D002175:Candida / Q000201:enzymology; D002208:Caproates / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D007783:Lactones / Q000737:chemistry; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D011091:Polyesters / Q000737:chemistry*
chemical_list: D002208:Caproates; D004800:Enzymes, Immobilized; D007783:Lactones; D011091:Polyesters; C016240:polycaprolactone; C121056:caprolactone; D008049:Lipase
keywords: Aqueous dispersion; Lipase; Ring-opening polymerization; ɛ-caprolactone