title: Fabrications of zinc-releasing biocement combining zinc calcium phosphate to calcium phosphate cement.
abstract: Recently, zinc-releasing bioceramics have been the focus of much attention owing to their bone-forming ability. Thus, some types of zinc-containing calcium phosphate (e.g., zinc-doped tricalcium phosphate and zinc-substituted hydroxyapatite) are examined and their osteoblastic cell responses determined. In this investigation, we studied the effects of zinc calcium phosphate (ZCP) derived from zinc phosphate incorporated into calcium phosphate cement (CPC) in terms of its setting reaction and MC3T3-E1 osteoblast-like cell responses. Compositional analysis by powder X-ray diffraction analysis revealed that HAP crystals were precipitated in the CPC containing 10 or 30wt% ZCP after successfully hardening. However, the crystal growth observed by scanning electron microscopy was delayed in the presence of additional ZCP. These findings indicate that the additional zinc inhibits crystal growth and the conversion of CPC to the HAP crystals. The proliferation of the cells and alkaline phosphatase (ALP) activity were enhanced when 10wt% ZCP was added to CPC. Taken together, ZCP added CPC at an appropriate fraction has a potent promotional effect on bone substitute biomaterials.
authors.affiliation: Department of Orthodontics and Dentofacial Orthopedics, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8504, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Horiuchi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hiasa
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yasue
authors.forename: Kazumitsu
authors.initials: K
authors.lastname: Sekine
authors.forename: Kenichi
authors.initials: K
authors.lastname: Hamada
authors.forename: Kenzo
authors.initials: K
authors.lastname: Asaoka
authors.forename: Eiji
authors.initials: E
authors.lastname: Tanaka
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001843:Bone Cements / Q000737:chemistry* / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D055595:Mechanical Phenomena; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D010710:Phosphates / Q000737:chemistry*; D013997:Time Factors; D015032:Zinc / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D001843:Bone Cements; D002130:Calcium Phosphates; D010710:Phosphates; D017967:Zinc Compounds; C043952:zinc phosphate; C020243:calcium phosphate; D000469:Alkaline Phosphatase; D015032:Zinc
keywords: Calcium phosphate cement; Hopeite; Hydroxyapatite (HAP); Synthetic cement; Zinc calcium phosphate; Zinc oxide