title: Establishment and evaluation of a stable cattle type II alveolar epithelial cell line.
abstract: Macrophages and dendritic cells are recognized as key players in the defense against mycobacterial infection. Recent research has confirmed that alveolar epithelial cells (AECs) also play important roles against mycobacterium infections. Thus, establishing a stable cattle AEC line for future endogenous immune research on bacterial invasion is necessary. In the present study, we first purified and immortalized type II AECs (AEC II cells) by transfecting them with a plasmid containing the human telomerase reverse trancriptase gene. We then tested whether or not the immortalized cells retained the basic physiological properties of primary AECs by reverse-transcription polymerase chain reaction and Western blot. Finally, we tested the secretion capacity of immortalized AEC II cells upon stimulation by bacterial invasion. The cattle type II alveolar epithelial cell line (HTERT-AEC II) that we established retained lung epithelial cell characteristics: the cells were positive for surfactants A and B, and they secreted tumor necrosis factor-α and interleukin-6 in response to bacterial invasion. Thus, the cell line we established is a potential tool for research on the relationship between AECs and Mycobacterium tuberculosis.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Su
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Guanghui
authors.initials: G
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yu
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Wang
authors.forename: Yaping
authors.initials: Y
authors.lastname: Jin
authors.forename: Guangdong
authors.initials: G
authors.lastname: Hu
authors.forename: Song
authors.initials: S
authors.lastname: Hua
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000382:microbiology*; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D004847:Epithelial Cells / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009164:Mycobacterium Infections / Q000276:immunology / Q000662:veterinary*; D010957:Plasmids / Q000235:genetics; D011650:Pulmonary Alveoli / Q000166:cytology*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019098:Telomerase / Q000235:genetics
chemical_list: D017931:DNA Primers; D019098:Telomerase