title: The in vivo blood compatibility of bio-inspired small diameter vascular graft: effect of submicron longitudinally aligned topography.
abstract: BACKGROUND
  Cardiovascular disease is the leading cause of deaths worldwide and the arterial reconstructive surgery remains the treatment of choice. Although large diameter vascular grafts have been widely used in clinical practices, there is an urgent need to develop a small diameter vascular graft with enhanced blood compatibility. Herein, we fabricated a small diameter vascular graft with submicron longitudinally aligned topography, which mimicked the tunica intima of the native arterial vessels and were tested in Sprague-Dawley (SD) rats.
  
  
  METHODS
  Vascular grafts with aligned and smooth topography were prepared by electrospinning and were connected to the abdominal aorta of the SD rats to evaluate their blood compatibility. Graft patency and platelet adhesion were evaluated by color Doppler ultrasound and immunofluorescence respectively.
  
  
  RESULTS
  We observed a significant higher patency rate (p = 0.021) and less thrombus formation in vascular graft with aligned topography than vascular graft with smooth topography. However, no significant difference between the adhesion rates on both vascular grafts (smooth/aligned: 0.35‰/0.12‰, p > 0.05) was observed. Moreover, both vascular grafts had few adherent activated platelets on the luminal surface.
  
  
  CONCLUSIONS
  Bionic vascular graft showed enhanced blood compatibility due to the effect of surface topography. Therefore, it has considerable potential for using in clinical application.
authors.affiliation: Department of Vascular Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, P, R, China. zuojunhu@hotmail.com.
authors.forename: Ruiming
authors.initials: R
authors.lastname: Liu
authors.forename: Yuansen
authors.initials: Y
authors.lastname: Qin
authors.forename: Huijin
authors.initials: H
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Zuojun
authors.initials: Z
authors.lastname: Hu
authors.forename: Shenming
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000473:pathology / Q000502:physiology / Q000601:surgery*; D001807:Blood Vessel Prosthesis / Q000592:standards; D019917:Blood Vessel Prosthesis Implantation / Q000295:instrumentation* / Q000379:methods* / Q000592:standards; D010973:Platelet Adhesiveness / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014654:Vascular Patency / Q000502:physiology*