title: Nspc1 regulates the key pluripotent Oct4-Nanog-Sox2 axis in P19 embryonal carcinoma cells via directly activating Oct4.
abstract: Nspc1 is an identified transcription repressor. However, transiently up-regulated or down-regulated Nspc1 in P19 embryonal carcinoma cells affects expression levels of Oct4, Sox2 and Nanog in a positive correlation. Luciferase activity assays verified that Nspc1 regulates the Oct4 promoter in a dose dependent manner. ChIP assay shows that Nspc1 activates Oct4 by directly binding to the (-1021 to -784) region of Oct4 promoter. Dominant negative analysis indicated the activation is dependent on the retinoid acid response element (RARE). We demonstrated Nspc1 has a positive role in maintaining the pluripotency of P19 cells by directly regulating Oct4.
authors.affiliation: Department of Histology and Embryology, Logistic University of the Chinese People's Armed Police Force, Tianjin 300162, China; Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Rong
authors.initials: R
authors.lastname: Fan
authors.forename: Mingze
authors.initials: M
authors.lastname: Sun
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Gong
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D018398:Homeodomain Proteins / Q000235:genetics*; D051379:Mice; D000071317:Nanog Homeobox Protein; D055495:Neurogenesis / Q000235:genetics*; D050814:Octamer Transcription Factor-3 / Q000235:genetics*; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D063150:Polycomb Repressive Complex 1 / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D055748:SOXB1 Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation
chemical_list: D018398:Homeodomain Proteins; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse; D050814:Octamer Transcription Factor-3; C570527:Pcgf1 protein, mouse; C494174:Pou5f1 protein, mouse; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; D063150:Polycomb Repressive Complex 1
keywords: Embryonal carcinoma cells; Oct4; Polycomb group protein; Transcription regulation