title: Neuroprotective effects of microRNA-210 on hypoxic-ischemic encephalopathy.
abstract: OBJECTIVE
  To reveal the effect of microRNA-210 on cell apoptosis caused by HIE.
  
  
  METHODS
  Postnatal day 7 rats after HI injury were intraventricularly injected with microRNA-210 mimic, microRNA-210 inhibitor, or physiological saline. 72 h after the injection, rats were sacrificed and the left hemispheres were collected. The expression level of microRNA-210 was identified by quantitative real-time PCR analysis. Apoptosis in brain sections was investigated by TUNEL assay. Apoptosis-related protein expressions were studied by Western blot analysis.
  
  
  RESULTS
  The results showed that microRNA-210, whose expression was downregulated in the brain 72 h after HI injury, suppressed neuronal apoptosis by inhibiting caspase activity and regulating the balance between bcl-2 and bax levels.
  
  
  CONCLUSIONS
  Recent study demonstrated that microRNA-210 has neuroprotective effects through inhibiting apoptosis in a murine model of HIE. It represents a potential novel therapeutic approach for the treatment of HIE.
authors.affiliation: Department of Newborn Infants, Nanjing Children's Hospital of Nanjing Medical University, Nanjing 210008, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Qiu
authors.forename: Xiao-yu
authors.initials: XY
authors.lastname: Zhou
authors.forename: Xiao-guang
authors.initials: XG
authors.lastname: Zhou
authors.forename: Rui
authors.initials: R
authors.lastname: Cheng
authors.forename: Hai-ying
authors.initials: HY
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D006801:Humans; D020925:Hypoxia-Ischemia, Brain / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000008:administration & dosage* / Q000037:antagonists & inhibitors / Q000138:chemical synthesis / Q000235:genetics; D018696:Neuroprotective Agents / Q000008:administration & dosage; D051381:Rats
chemical_list: C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D018696:Neuroprotective Agents