title: Piezoelectric tuning fork probe for atomic force microscopy imaging and specific recognition force spectroscopy of an enzyme and its ligand.
abstract: Piezoelectric quartz tuning fork has drawn the attention of many researchers for the development of new atomic force microscopy (AFM) self-sensing probes. However, only few works have been done for soft biological materials imaging in air or aqueous conditions. The aim of this work was to demonstrate the efficiency of the AFM tuning fork probe to perform high-resolution imaging of proteins and to study the specific interaction between a ligand and its receptor in aqueous media. Thus, a new kind of self-sensing AFM sensor was introduced to realize imaging and biochemical specific recognition spectroscopy of glucose oxidase enzyme using a new chemical functionalization procedure of the metallic tips based on the electrochemical reduction of diazonium salt. This scanning probe as well as the functionalization strategy proved to be efficient respectively for the topography and force spectroscopy of soft biological materials in buffer conditions.
authors.affiliation: CEA, IRAMIS, Service de Physique et Chimie des Surfaces et Interfaces, Gif-sur-Yvette, France.
authors.forename: Ali
authors.initials: A
authors.lastname: Makky
authors.forename: Pascal
authors.initials: P
authors.lastname: Viel
authors.forename: Shu-wen Wendy
authors.initials: SW
authors.lastname: Chen
authors.forename: Thomas
authors.initials: T
authors.lastname: Berthelot
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Pellequer
authors.forename: Jérôme
authors.initials: J
authors.lastname: Polesel-Maris
mesh_terms: D000327:Adsorption; D000388:Air; D000538:Aluminum Silicates; D001234:Aspergillus niger / Q000201:enzymology*; D003198:Computer Simulation; D055664:Electrochemical Techniques; D005944:Glucosamine / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism*; D008024:Ligands; D018625:Microscopy, Atomic Force / Q000379:methods*
chemical_list: D000538:Aluminum Silicates; D008024:Ligands; D005949:Glucose Oxidase; D005944:Glucosamine; C011934:mica
keywords: atomic force microscopy; force spectroscopy; glucose oxidase; tuning fork