title: The origin of amniotic polymorphonuclear leucocytes in the mare.
abstract: The objective of this study was to investigate the presence and origin of polymorphonuclear leucocytes (PMNLs) in the amniotic fluid of mares giving birth to healthy foals. Material from 25 mares was included. Amniotic fluid was collected during parturition before breakage of the amniotic vesicle. Manual microscopic cytologic evaluation was made on cytospin preparations after staining. PMNLs were found in all amniotic samples examined. The genomic DNA was extracted from 12 of the amniotic fluid samples and was genotyped. The results indicate that the PMNLs originate from the foetus.
authors.affiliation: Herrgården, Hjortkvarn, Sweden; Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Hemberg
authors.forename: S
authors.initials: S
authors.lastname: Einarsson
authors.forename: B
authors.initials: B
authors.lastname: Jones
authors.forename: S
authors.initials: S
authors.lastname: Mikko
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology*; D000818:Animals; D005260:Female; D005333:Fetus / Q000166:cytology; D006736:Horses / Q000502:physiology*; D009504:Neutrophils / Q000502:physiology*; D011247:Pregnancy