title: [MicroRNAs regulate epithelial-mesenchymal transition of supraglottic laryngeal cancer].
abstract: OBJECTIVE
  To study microRNAs (miRNAs) expression profiles associated with epithelial-mesenchymal transition (EMT) in lymph node metastasis of supraglottic laryngeal squamous cell carcinomas(SGLSCC).
  
  
  METHODS
  Primary tumor tissue samples of 12 SGLSCC patients were collected, including 6 patients clinically diagnosed with lymph nodes metastasis (N(+)) and 6 patients with lymph nodes metastasis-free (N0), for miRNA microarray gene-expression profiling to identify the differences between N(+) and N0 groups. Differentially expressed miRNAs was verified using quantitative real-time PCR in 20 patients with N(+) and 20 patients with N0. Target genes for the miRNAs associated with EMT in SGLSCC metastasis were analyzed.
  
  
  RESULTS
  Ten miRNAs differentially expressed between N(+) group and N0 group were determined. Comparing with N0 group, nine miRNAs were over-expressed and one miRNA was expressed at lower level in N(+) group. The genes for miR-192, miR-143, miR-409 and miR-634 were predicted as target genes that could promote EMT of laryngeal cancer cells by targeted inhibiting Krüppel-like factor 17(KLF17), E-cadherin and phosphatidylinositol 3 kinase (PI3K).
  
  
  CONCLUSIONS
  The miRNAs over-expressed in group N(+) can be used to predict cervical lymph node metastasis in SGLSCC. The miRNAs as new markers could improve the diagnosis and treatment of SGLSCC.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education (Capital Medical University), Beijing 100730, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tai
authors.forename: Xiao
authors.initials: X
authors.lastname: Xiao
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Huang
authors.forename: Zhen-kun
authors.initials: ZK
authors.lastname: Yu
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Chen
authors.forename: Wei-guo
authors.initials: WG
authors.lastname: Zhou
authors.forename: Xue-jun
authors.initials: XJ
authors.lastname: Chen
authors.forename: Yuan-sheng
authors.initials: YS
authors.lastname: Rao
authors.forename: Ju-gao
authors.initials: JG
authors.lastname: Fang
authors.forename: Xin
authors.initials: X
authors.lastname: Ni
mesh_terms: D000368:Aged; D015820:Cadherins; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D020869:Gene Expression Profiling; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000378:metabolism; D007830:Larynx; D007831:Larynx, Artificial; D008198:Lymph Nodes; D008207:Lymphatic Metastasis / Q000235:genetics; D035683:MicroRNAs / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D015820:Cadherins; D035683:MicroRNAs; D019869:Phosphatidylinositol 3-Kinases