title: Biodegradation of decabromodiphenyl ether (BDE-209) by a metal resistant strain, Bacillus cereus JP12.
abstract: A metal resistant bacterial strain, Bacillus cereus JP12, could use decabromodiphenyl ether (BDE-209) as the sole carbon and energy source for growth in mineral salt medium. Under the conditions of pH 6.0, 30°C, 150 rpm and an inoculum of OD600=0.6, more than 88% of the initial BDE-209 (1mg/L) was degraded after 12 days. The addition of appropriate surfactants and additional carbon sources could enhance the biodegradation efficiency of BDE-209. The presence of Cu(2+) (≤ 8 mg/L) and Zn(2+) (≤ 15 mg/L) provided a slight stimulating effect on BDE-209 removal. However, BDE-209 biodegradation efficiency was decreased when adding higher levels of metals due to reduced substrate availability caused by excess metal adsorption into the cell surface. Biosorption of heavy metals by JP12 led to release of light metals such as K(+) and Na(+). A BDE-209 biodegradation pathway was proposed on the basis of metabolite identification.
authors.affiliation: State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing 102249, China; School of Materials Science and Engineering, Jingdezhen Ceramic Institute, Jingdezhen 333403, Jiangxi Province, China.
authors.forename: Mang
authors.initials: M
authors.lastname: Lu
authors.forename: Zhong-Zhi
authors.initials: ZZ
authors.lastname: Zhang
authors.forename: Xue-Jiao
authors.initials: XJ
authors.lastname: Wu
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Xu
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Su
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Jing-Xiu
authors.initials: JX
authors.lastname: Wang
mesh_terms: D000332:Aerobiosis / Q000187:drug effects; D001409:Bacillus cereus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D055768:Halogenated Diphenyl Ethers / Q000737:chemistry / Q000378:metabolism*; D054879:Halogenation / Q000187:drug effects; D019216:Metals, Heavy / Q000633:toxicity*; D017550:Spectroscopy, Fourier Transform Infrared; D013501:Surface-Active Agents / Q000494:pharmacology; D013997:Time Factors
chemical_list: D055768:Halogenated Diphenyl Ethers; D019216:Metals, Heavy; D013501:Surface-Active Agents; C010902:decabromobiphenyl ether
keywords: Aerobic debromination; BDE-209; Heavy metals; Metabolites