title: Enhanced lymphatic transport of bioactive lipids: cell culture study of polymethoxyflavone incorporation into chylomicrons.
abstract: Polymethoxyflavones (PMFs) are bioactive flavonoids found in citrus fruits that have been shown to have potential health promoting properties. However, their application as nutraceuticals in functional foods and beverages is currently limited due to their low water solubility and high melting point. The oral bioavailability of lipophilic compounds can be enhanced by promoting their intestinal lymphatic transport through co-administration with digestible lipids. We investigated the effects of chylomicron-mediated intestinal lymphatic transport on the bioavailability of 5-hydroxy-6,7,8,3',4'-pentamethoxylflavone (5-HPMF), one of representative PMFs in Caco-2 cells. Our results demonstrated that oleic acid and bile acid promoted secretion of chylomicrons in Caco-2 cells, with mean diameter ranged from 70 to 150 nm. The intracellular level of 5-HPMF increased 3-fold by co-incubation with the mixed micelle solution. Moreover, the basolateral level of 5-HPMF increased 3-fold due to enhanced chylomicron-mediated transport. Overall, our results demonstrated for the first time that the bioavailability of polymethoxyflavones can be enhanced by promoting their incorporation into chylomicrons.
authors.affiliation: Department of Food Science, University of Massachusetts, 102 Holdsworth Way Chenoweth, Amherst, Massachusetts 01003, USA. hangxiao@foodsci.umass.edu.
authors.forename: Mingfei
authors.initials: M
authors.lastname: Yao
authors.forename: Jingjing
authors.initials: J
authors.lastname: Chen
authors.forename: Jinkai
authors.initials: J
authors.lastname: Zheng
authors.forename: Mingyue
authors.initials: M
authors.lastname: Song
authors.forename: David Julian
authors.initials: DJ
authors.lastname: McClements
authors.forename: Hang
authors.initials: H
authors.lastname: Xiao
mesh_terms: D001692:Biological Transport; D018938:Caco-2 Cells; D002914:Chylomicrons / Q000378:metabolism*; D002957:Citrus / Q000737:chemistry; D020895:Enterocytes / Q000378:metabolism*; D005419:Flavonoids / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D008196:Lymph / Q000378:metabolism*; D019301:Oleic Acid / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism*
chemical_list: D002914:Chylomicrons; D005419:Flavonoids; D010936:Plant Extracts; D019301:Oleic Acid