title: Histone acetyltransferase p300 promotes MKL1-mediated transactivation of catechol-O-methyltransferase gene.
abstract: Previous studies have revealed that histone acetyltransferase p300 is recruited to the promoters of certain cardiac and smooth muscle specific genes to enhance the transactivation activity of myocardin, which is a master regulator in cardiovascular differentiation and development. Here, we found that the gene encoding catechol-O-methyltransferase (COMT), an important metabolic enzyme catalyzing the conversion of estrogen, is also a target gene of myocardin-related transcription factors (MRTFs). Megakaryoblastic leukemia 1 (MKL1, also named MRTF-A) and p300 could synergistically augment the expression of COMT gene, increase the metabolic rate of estrogen, and thus reduce the proliferation of MCF-7 breast cancer cells stimulated by estrogen.
authors.affiliation: Key Laboratory of Industrial Microbiology, Ministry of Education and Tianjin City, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Liu
authors.forename: Xuegang
authors.initials: X
authors.lastname: Luo
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Wenwen
authors.initials: W
authors.lastname: Zhao
authors.forename: Shu
authors.initials: S
authors.lastname: Guo
authors.forename: Yu
authors.initials: Y
authors.lastname: Guo
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Hongpeng
authors.initials: H
authors.lastname: He
authors.forename: Xinghua
authors.initials: X
authors.lastname: Liao
authors.forename: Wenjian
authors.initials: W
authors.lastname: Ma
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.forename: Tongcun
authors.initials: T
authors.lastname: Zhang
mesh_terms: D048729:Anacardic Acids / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D019556:COS Cells; D002394:Catechol O-Methyltransferase / Q000235:genetics* / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism / Q000494:pharmacology; D004967:Estrogens / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D061986:MCF-7 Cells; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D015514:Oncogene Proteins, Fusion / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators; D015533:Transcriptional Activation / Q000187:drug effects / Q000235:genetics*; D050880:p300-CBP Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D000099224:p300-CBP-Associated Factor
chemical_list: D048729:Anacardic Acids; D002394:Catechol O-Methyltransferase; D004268:DNA-Binding Proteins; D004958:Estradiol; D004967:Estrogens; D008156:Luciferases; D015514:Oncogene Proteins, Fusion; D015534:Trans-Activators; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor; C431575:MRTFA protein, human; C088115:anacardic acid; C588010:COMT protein, human
keywords: COMT; MKL1; cell metabolism; p300; transcriptional regulation