title: Vigna angularis inhibits IL-6-induced cellular signalling and ameliorates collagen-induced arthritis.
abstract: OBJECTIVE
  The present study was conducted in order to assess whether extracts or isolated compounds from Vigna angularis were able to suppress IL-6 signalling and to show the therapeutic effect on collagen-induced arthritis (CIA) in mice.
  
  
  METHODS
  The effect of V. angularis on IL-6 signalling was studied by measuring Stat3-dependent luciferase activity, expression of inflammation-related genes, and phosphorylation of Janus kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3) and extracellular signal-regulated kinase (ERK) induced by IL-6. CIA was induced by immunizing with bovine type II collagen. V. angularis extract (VAE) was administrated orally at 50 and 100 mg/kg from day 1 to day 28. Induction of arthritis was evaluated with a visual scoring system and histological analysis.
  
  
  RESULTS
  Extracts or two triterpenoid compounds from V. angularis showed potent inhibitory effects on pSTAT3-inducible luciferase activity, STAT3 tyrosine phosphorylation and the expression of inflammation-related genes induced by IL-6. Administration of VAE significantly suppressed the progression of CIA, accompanied by a reduced antibody response to type II collagen and protection from tissue damage in knee joints.
  
  
  CONCLUSIONS
  Administration of VAE has a therapeutic effect on CIA and this effect is associated with the inhibitory activity on IL-6/STAT3 signalling. These results suggest that extracts or compounds from V. angularis could be a useful treatment for diseases related to IL-6, including RA.
authors.affiliation: Bioindustrial Process Research Center, Bio-Materials Research Institute, KRIBB, 1404 Sinjeong-dong, Jeongeup-si, Jeonbuk 580-185, Republic of Korea. rho-m@kribb.re.kr.
authors.forename: Hyun-Mee
authors.initials: HM
authors.lastname: Oh
authors.forename: Seung Woong
authors.initials: SW
authors.lastname: Lee
authors.forename: Bo Ra
authors.initials: BR
authors.lastname: Yun
authors.forename: Byung Soon
authors.initials: BS
authors.lastname: Hwang
authors.forename: So Nam
authors.initials: SN
authors.lastname: Kim
authors.forename: Chan Sun
authors.initials: CS
authors.lastname: Park
authors.forename: See-Hwa
authors.initials: SH
authors.lastname: Jeoung
authors.forename: Hyun-Kyu
authors.initials: HK
authors.lastname: Kim
authors.forename: Woo Song
authors.initials: WS
authors.lastname: Lee
authors.forename: Mun-Chual
authors.initials: MC
authors.lastname: Rho
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D015153:Blotting, Western; D002417:Cattle; D003094:Collagen / Q000633:toxicity; D005786:Gene Expression Regulation; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D010936:Plant Extracts / Q000008:administration & dosage / Q000627:therapeutic use*; D012313:RNA / Q000235:genetics*; D050796:STAT3 Transcription Factor / Q000096:biosynthesis / Q000235:genetics*; D015398:Signal Transduction / Q000187:drug effects; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D015850:Interleukin-6; D010936:Plant Extracts; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D014409:Tumor Necrosis Factor-alpha; D012313:RNA; D003094:Collagen
keywords: IL-6; STAT3; Vigna angularis; collagen-induced arthritis; rheumatoid arthritis