title: Interferon α/β receptor knockout mice as a model to study bluetongue virus infection.
abstract: Bluetongue is an arthropod-borne disease caused by a virus of the genus Orbivirus, the bluetongue virus (BTV), which affects ruminant livestock such as cattle, sheep, and goats and wild ruminants such as deer, and camelids. Recently, adult mice with gene knockouts of the interferon α/β receptor (IFNAR-/-) have been described as a model of lethal BTV infection. IFNAR(-/-) mice are highly susceptible to BTV-1, BTV-4 and BTV-8 infection when the virus is administered intravenously or subcutaneosuly. Disease progression and pathogenesis closely mimics signs of bluetongue disease in ruminants. In the present paper we review the studies where IFNAR(-/-) mice have been used as an animal model to study BTV transmission, pathogenesis, virulence, and protective efficacy of inactivated and new recombinant marker BTV vaccines. Furthermore, we report new data on protective efficacy of different strategies of BTV vaccination and also on induction of interferon α/β and proinflammatory immune responses in IFNAR(-/-) mice infected with BTV.
authors.affiliation: Centro de Investigación en Sanidad Animal, CISA-INIA, Valdeolmos, Madrid, Spain. Electronic address: ortego@inia.es.
authors.forename: Javier
authors.initials: J
authors.lastname: Ortego
authors.affiliation: Centro de Investigación en Sanidad Animal, CISA-INIA, Valdeolmos, Madrid, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: de la Poza
authors.affiliation: Centro de Investigación en Sanidad Animal, CISA-INIA, Valdeolmos, Madrid, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Marín-López
mesh_terms: D000818:Animals; D001819:Bluetongue / Q000276:immunology / Q000473:pathology* / Q000517:prevention & control / Q000821:virology*; D001820:Bluetongue virus / Q000276:immunology* / Q000472:pathogenicity / Q000502:physiology*; D004195:Disease Models, Animal; D051379:Mice; D018345:Mice, Knockout; D053633:Receptor, Interferon alpha-beta / Q000172:deficiency*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology
chemical_list: D014765:Viral Vaccines; D053633:Receptor, Interferon alpha-beta
keywords: Bluetongue virus; IFNAR(−/−) mice; Orbiviruses