title: HDAC5 promotes osteosarcoma progression by upregulation of Twist 1 expression.
abstract: Histone deacetylases (HDACs) form a family of enzymes, which have fundamental roles in the epigenetic regulation of gene expression and contribute to the growth, differentiation, and apoptosis of cancer cells. In this study, we firstly investigated the biological function of HDAC5 in osteosarcoma cells. We found that mRNA and protein levels of HDAC5 were upregulated in osteosarcoma tissues and cell lines. Furthermore, overexpression of HDAC5 could promote cell proliferation in osteosarcoma cell lines. In contrast, HDAC5 knockdown using small interfering RNA inhibited cell proliferation. At the molecular level, we demonstrated that HDAC5 promoted mRNA expression of twist 1, which has been reported as an oncogene. Together, these results highlighted for the first time an unrecognized link between HDAC5 and osteosarcoma progression and demonstrated that its specific inhibition might contribute to the treatment of tumorigenesis.
authors.affiliation: Department of Orthopedic, Huashan Hospital, Fudan University, Shanghai, 200040, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Xia
authors.forename: Yong-lin
authors.initials: YL
authors.lastname: Yu
authors.forename: Si-qun
authors.initials: SQ
authors.lastname: Wang
authors.forename: Yi-bing
authors.initials: YB
authors.lastname: Wei
authors.forename: Fei-yan
authors.initials: FY
authors.lastname: Chen
authors.forename: Gang-yong
authors.initials: GY
authors.lastname: Huang
authors.forename: Jing-sheng
authors.initials: JS
authors.lastname: Shi
mesh_terms: D017209:Apoptosis / Q000235:genetics; D063646:Carcinogenesis / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D006655:Histone Deacetylases / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D051879:Twist-Related Protein 1 / Q000096:biosynthesis / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D009687:Nuclear Proteins; D012333:RNA, Messenger; C103173:TWIST1 protein, human; D051879:Twist-Related Protein 1; C404500:HDAC5 protein, human; D006655:Histone Deacetylases