title: High-efficiency localization of Na(+)-K(+) ATPases on the cytoplasmic side by direct stochastic optical reconstruction microscopy.
abstract: We describe a concise and effective strategy towards precisely mapping Na(+)-K(+) ATPases on the cytoplasmic side of cell membranes by direct stochastic optical reconstruction microscopy (dSTORM). We found that most Na(+)-K(+) ATPases are localized in different sizes of clusters on human red blood cell (hRBC) membranes, revealed by Ripley's K-function analysis. Further evidence that cholesterol depletion causes the dispersion of Na(+)-K(+) ATPase clusters indicates that such clusters could be localized in cholesterol-enriched domains. Our results suggest that Na(+)-K(+) ATPases might aggregate within the lipid rafts to fulfill their functions.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P.R. China. hdwang@ciac.ac.cn jiangjg@ciac.ac.cn.
authors.forename: Jiazhen
authors.initials: J
authors.lastname: Wu
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.forename: Miao
authors.initials: M
authors.lastname: Qi
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Wang
authors.forename: Mingjun
authors.initials: M
authors.lastname: Cai
authors.forename: Shuheng
authors.initials: S
authors.lastname: Liu
authors.forename: Xian
authors.initials: X
authors.lastname: Hao
authors.forename: Junguang
authors.initials: J
authors.lastname: Jiang
authors.forename: Hongda
authors.initials: H
authors.lastname: Wang
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D002232:Carbocyanines / Q000737:chemistry; D002784:Cholesterol / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism*; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008853:Microscopy; D049329:Nanostructures / Q000737:chemistry; D010316:Particle Size; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D002232:Carbocyanines; C085321:cyanine dye 5; D002784:Cholesterol; D000254:Sodium-Potassium-Exchanging ATPase