title: Immunofluorescence microscopy for the detection of surface antigens in methicillin-resistant Staphylococcus aureus (MRSA).
abstract: Immunofluorescence microscopy is a widely used laboratory method which allows detection and visualization of specific antigens. The method employs the specificity of antibodies to deliver fluorophore to a specific target and then visualize it with a microscope. The power of the technique is that it requires relatively little manipulation and relatively few bacterial cells, enabling the detection of antigen expression where other methods cannot, such as during an actual infection in an animal. Here, we apply the method to follow antigen expression on the surface of MRSA cells over time in in vivo infection models.
authors.affiliation: Pfizer Vaccine Research, Pearl River, NY, USA.
authors.forename: Yekaterina
authors.initials: Y
authors.lastname: Timofeyeva
authors.forename: Ingrid L
authors.initials: IL
authors.lastname: Scully
authors.forename: Annaliesa S
authors.initials: AS
authors.lastname: Anderson
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D005455:Fluorescent Antibody Technique / Q000379:methods; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D013203:Staphylococcal Infections / Q000276:immunology / Q000382:microbiology*
chemical_list: D000942:Antigens, Bacterial; D000954:Antigens, Surface