title: Time-resolved fluorescence anisotropy imaging.
abstract: Fluorescence can be characterized by its intensity, position, wavelength, lifetime, and polarization. The more of these features are acquired in a single measurement, the more can be learned about the sample, i.e., the microenvironment of the fluorescence probe. Polarization-resolved fluorescence lifetime imaging-time-resolved fluorescence anisotropy imaging, TR-FAIM-allows mapping of viscosity or binding or of homo-FRET which can indicate dimerization or generally oligomerization.
authors.affiliation: Department of Physics, King's College London, London, UK.
authors.forename: Klaus
authors.initials: K
authors.lastname: Suhling
authors.forename: James
authors.initials: J
authors.lastname: Levitt
authors.forename: Pei-Hua
authors.initials: PH
authors.lastname: Chung
mesh_terms: D019281:Dimerization; D005454:Fluorescence Polarization / Q000379:methods*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D061848:Optical Imaging; D014783:Viscosity