title: Primary cilia respond to fluid shear stress and mediate flow-induced calcium deposition in osteoblasts.
abstract: Bone turnover in vivo is regulated by mechanical forces such as shear stress originating from interstitial oscillatory fluid flow (OFF), and bone cells in vitro respond to mechanical loading. However, the mechanisms by which bone cells sense mechanical forces, resulting in increased mineral deposition, are not well understood. The aim of this study was to investigate the role of the primary cilium in mechanosensing by osteoblasts. MLO-A5 murine osteoblasts were cultured in monolayer and subjected to two different OFF regimens: 5 short (2 h daily) bouts of OFF followed by morphological analysis of primary cilia; or exposure to chloral hydrate to damage or remove primary cilia and 2 short bouts (2 h on consecutive days) of OFF. Primary cilia were shorter and there were fewer cilia per cell after exposure to periods of OFF compared with static controls. Damage or removal of primary cilia inhibited OFF-induced PGE2 release into the medium and mineral deposition, assayed by Alizarin red staining. We conclude that primary cilia are important mediators of OFF-induced mineral deposition, which has relevance for the design of bone tissue engineering strategies and may inform clinical treatments of bone disorders causes by load-deficiency.
authors.affiliation: 1School of Engineering and Materials Science, Queen Mary University of London, Mile End Rd., London, E1 4NS, UK. r.delaine-smith@qmul.ac.uk.
authors.forename: Robin M
authors.initials: RM
authors.lastname: Delaine-Smith
authors.forename: Anuphan
authors.initials: A
authors.lastname: Sittichokechaiwut
authors.forename: Gwendolen C
authors.initials: GC
authors.lastname: Reilly
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D002923:Cilia / Q000378:metabolism* / Q000502:physiology*; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism*; D013314:Stress, Mechanical
chemical_list: D002118:Calcium
keywords: extracellular matrix; mechanotransduction; oscillatory fluid flow; osteogenesis