title: Anti-inflammatory activity of iridoids and verbascoside isolated from Castilleja tenuiflora.
abstract: Castilleja tenuiflora (Orobanchaceae) has been used in Mexican traditional medicine as a treatment for cough, dysentery, anxiety, nausea and vomiting as well as hepatic and gastrointestinal diseases. The ethanolic extract of the aerial parts of Castilleja tenuiflora was separated by silica gel column chromatography. The fractions were evaluated using the induced edema acetate 12-O-tetradecanoylphorbol (TPA) anti-inflammatory activity model. The most active fraction was subjected to medium-pressure liquid chromatography (MPLC) with UV detection at 206 and 240 nm. The following iridoids were isolated: geniposidic acid, aucubin, bartioside, 8-epi-loganin, mussaenoside, and the phenylpropanoid verbascoside. The most active iridoid was geniposidic acid, which was more active than the control (indomethacin), and the least active iridoid was mussaenoside. 8-epi-Loganin, and mussaenoside have not been previously reported to be anti-inflammatory compounds. The results of these investigations confirm the potential of Mexican plants for the production of bioactive compounds and validate the ethnomedical use of Castilleja tenuiflora-like anti-inflammatory plants.
authors.affiliation: Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa., Cuernavaca 62209, Morelos, Mexico. veronica_rodriguez@uaem.mx.
authors.forename: Danae
authors.initials: D
authors.lastname: Carrillo-Ocampo
authors.forename: Sugeyla
authors.initials: S
authors.lastname: Bazaldúa-Gómez
authors.forename: Jaime R
authors.initials: JR
authors.lastname: Bonilla-Barbosa
authors.forename: Rola
authors.initials: R
authors.lastname: Aburto-Amar
authors.forename: Verónica
authors.initials: V
authors.lastname: Rodríguez-López
mesh_terms: D027901:Acanthaceae / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004487:Edema / Q000188:drug therapy; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D039823:Iridoids / Q000302:isolation & purification / Q000494:pharmacology*; D008519:Medicine, Traditional; D051379:Mice; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects; D059808:Polyphenols
chemical_list: D000893:Anti-Inflammatory Agents; D005960:Glucosides; D039823:Iridoids; D010636:Phenols; D010936:Plant Extracts; C058956:acteoside; D059808:Polyphenols