title: Fixation of CO2 in Clostridium cellulovorans analyzed by 13C-isotopomer-based target metabolomics.
abstract: Clostridium cellulovorans has been one of promising microorganisms to use biomass efficiently; however the basic metabolic pathways have not been completely known. We carried out 13C-isotopomer-based target metabolome analysis, or carbohydrate conversion process analysis, for more profound understanding of metabolic pathways of the bacterium. Our findings that pyruvate + oxaloacetate, fumarate, and malate inside and outside cells exhibited 13C incorporation suggest that C. cellulovorans exactly fixed CO2 and partly operated the TCA cycle in a reductive manner. Accompanied with CO2 fixation, the microorganism was also found to produce and secrete lactate. Overall, our study demonstrates that a part of C. cellulovorans metabolic pathways related to glycolysis and the TCA cycle are involved in CO2 fixation.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. miueda@kais.kyoto-u.ac.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Shinohara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakuragi
authors.forename: Hironobu
authors.initials: H
authors.lastname: Morisaka
authors.forename: Hideo
authors.initials: H
authors.lastname: Miyake
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tamaru
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Fukusaki
authors.forename: Kouichi
authors.initials: K
authors.lastname: Kuroda
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Ueda