title: Screening and antiviral analysis of phages that display peptides with an affinity to subunit C of porcine aminopeptidase.
abstract: The purified C subunit of the recombinant porcine aminopeptidase N (rpAPN-C) protein was used as an immobilized target to screen potential ligands against rpAPN-C from a 12-mer phage display random peptide library. After five rounds of biopanning, five phage clones showed specific binding affinities to rpAPN-C. In 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assays, the phage clone PM1, which contained the HDAISWTHYHPW peptide sequence, had a protective effect against TGEV infection in swine testis cells. Therefore, the HDAISWTHYHPW peptide sequence has a potential use as a small molecular therapeutic agent against TGEV infection.
authors.affiliation: 1 Department of Veterinary Clinical Medicine, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University , Daqing, P.R. China .
authors.forename: Donghua
authors.initials: D
authors.lastname: Guo
authors.forename: Qinghe
authors.initials: Q
authors.lastname: Zhu
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.forename: Dongbo
authors.initials: D
authors.lastname: Sun
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D018826:CD13 Antigens / Q000235:genetics*; D061505:Cell Surface Display Techniques; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005761:Gastroenteritis, Transmissible, of Swine / Q000188:drug therapy*; D008297:Male; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000235:genetics / Q000627:therapeutic use*; D019151:Peptide Library; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D013737:Testis / Q000821:virology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D005760:Transmissible gastroenteritis virus / Q000378:metabolism* / Q000502:physiology; D053586:Virus Internalization
chemical_list: D009842:Oligopeptides; D019151:Peptide Library; D021122:Protein Subunits; D013778:Tetrazolium Salts; D013844:Thiazoles; D018826:CD13 Antigens; C022616:thiazolyl blue