title: Rare-earth-incorporated polymeric vector for enhanced gene delivery.
abstract: Cationic polymer PEI-CyD is doped with Nd by plasma technology to produce the gene vector: Nd@PEI-CyD. Luciferase expression and EGFP transfection experiments performed in vitro reveal that Nd@PEI-CyD has significantly higher transfection efficiency than lipofectamine 2000 and PEI-CyD and the mechanism is studied and proposed. The rare-earth element, Nd, stimulates the energy metabolism of cells, enhances cell uptake of complexes/pDNA, and regulates the cellular pathways. These special features suggest a new strategy involving metal-incorporated non-viral gene vectors.
authors.affiliation: Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou 310028, PR China.
authors.forename: Qiwen
authors.initials: Q
authors.lastname: Wang
authors.forename: Weihong
authors.initials: W
authors.lastname: Jin
authors.forename: Guosong
authors.initials: G
authors.lastname: Wu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xue
authors.initials: X
authors.lastname: Jin
authors.forename: Xiurong
authors.initials: X
authors.lastname: Hu
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Guping
authors.initials: G
authors.lastname: Tang
authors.forename: Paul K
authors.initials: PK
authors.lastname: Chu
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D057809:HEK293 Cells; D006801:Humans; D008674:Metals, Rare Earth / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D011108:Polymers / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D008674:Metals, Rare Earth; D011108:Polymers
keywords: Cellular pathways; Energetic ions; Neodymium; PEI-CyD; Plasma treatment