title: Arg-Thz is a minimal substrate for the N(α),N(α)-arginyl methyltransferase involved in the biosynthesis of plantazolicin.
abstract: The final biosynthetic step towards plantazolicin (PZN) comprises N(α),N(α)-arginyl methyltransferase (PznL) mediated N-terminal bismethylation. We show that PznL processes truncated desmethyl-plantazolicin analogues, but only those with an N-terminal guanidine side chain derived from arginine. PznL specificity, which is narrow, depends on the side chain of the N-terminal amino acid linked to an azole, and not so much on the number of azoles.
authors.affiliation: Technische Universität Berlin, Institut für Chemie, Straße des 17. Juni 124, 10623 Berlin, Germany. suessmuth@chem.tu-berlin.de.
authors.forename: Natalia A
authors.initials: NA
authors.lastname: Piwowarska
authors.forename: Srinivas
authors.initials: S
authors.lastname: Banala
authors.forename: Hermen S
authors.initials: HS
authors.lastname: Overkleeft
authors.forename: Roderich D
authors.initials: RD
authors.lastname: Süssmuth
mesh_terms: D001120:Arginine / Q000031:analogs & derivatives* / Q000378:metabolism; D001393:Azoles / Q000378:metabolism*; D008780:Methyltransferases / Q000378:metabolism*; D009842:Oligopeptides / Q000096:biosynthesis*; D013379:Substrate Specificity; D013844:Thiazoles / Q000378:metabolism
chemical_list: D001393:Azoles; D009842:Oligopeptides; D013844:Thiazoles; D001120:Arginine; D008780:Methyltransferases