title: Improving catalytic hydrolysis reaction efficiency of sol-gel-encapsulated Candida rugosa lipase with magnetic β-cyclodextrin nanoparticles.
abstract: A silica-based β-cyclodextrin was immobilized on magnetic nanoparticles to obtain a macrocyclic compound with magnetic property. Then, the β-cyclodextrin-grafted magnetic nanoparticles were encapsulated with Candida rugosa lipase in sol-gel matrices using alkoxysilane precursors. The catalytic activity of the encapsulated lipases was evaluated with model reactions, i.e., the hydrolysis of p-nitro-phenylpalmitate (p-NPP) and the enantioselective hydrolysis of rasemic Naproxen methyl ester that was studied in an aqueous buffer solution/isooctane reaction system. The results indicate that the cyclodextrin-based, encapsulated lipase particularly exhibited high conversion and enantioselectivity behavior compared to the sol-gel free lipase. It was also observed that excellent enantioselectivity (E=399) was obtained for the encapsulated lipase with magnetic β-cyclodextrin that has an ee value of S-Naproxen acid of about 98%.
authors.affiliation: Department of Chemistry, Selcuk University, 42075 Konya, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Ozyilmaz
authors.affiliation: Department of Chemistry, Selcuk University, 42075 Konya, Turkey.
authors.forename: Serkan
authors.initials: S
authors.lastname: Sayin
authors.affiliation: Department of Chemistry, Kirklareli University, Kirklareli 39000, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Arslan
authors.affiliation: Department of Chemistry, Selcuk University, 42075 Konya, Turkey. Electronic address: myilmaz42@yahoo.com.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Yilmaz
mesh_terms: D002175:Candida / Q000201:enzymology*; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D008689:Methacrylates; D053758:Nanoparticles / Q000737:chemistry*; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D008689:Methacrylates; C483029:Temphase; D047392:beta-Cyclodextrins; D008049:Lipase; C031215:betadex
keywords: Cyclodextrin; Drug; Enantioselectivity; Fe(3)O(4); Lipase