title: In vitro efficacy of various topical antimicrobial agents in different time periods from contamination to application against 6 multidrug-resistant bacterial strains isolated from burn patients.
abstract: OBJECTIVE
  In vitro efficacy evaluation of eleven topical antimicrobials against multidrug-resistant (MDR) bacteria isolated from burn wounds of our patients.
  
  
  METHODS
  Growth of six MDR bacterial strains: Pseudomonas aeruginosa (2 strains), Staphylococcus aureus, Staphylococcus haemolyticus, Enterococcus faecalis and Escherichia coli in burn-wound models was evaluated 24-h after application of the tested agents. Four different wound models were created to investigate the role of time elapsed between inoculation of bacteria and application of the agents on their antimicrobial activity and efficacy.
  
  
  RESULTS
  The efficacy against all the 6 bacteria in freshly contaminated wounds was excellent in majority of the tested agents. The longer was the time interval between inoculation and application of the topical antimicrobial agents, the higher failure of the agents was observed.
  
  
  CONCLUSIONS
  Topical antimicrobials play an important role in treatment of burn wounds, but they should be used according to their efficacy against bacterial strains present in patients' wounds. In cases where topical agents have been applied after 24 h, when formation of mature biofilm in the wound could be expected, it would probably not be possible to kill all the bacteria using topical antimicrobial therapy only.
authors.affiliation: Teaching Department of Burns and Reconstructive Surgery, Medical Faculty of Comenius University and University Hospital, Bratislava, Slovakia. Electronic address: mariannahajska@gmail.com.
authors.forename: Marianna
authors.initials: M
authors.lastname: Hajská
authors.affiliation: Institute of Microbiology, Medical Faculty of Comenius University and University Hospital, Bratislava, Slovakia.
authors.forename: Lívia
authors.initials: L
authors.lastname: Slobodníková
authors.affiliation: Institute of Microbiology, Medical Faculty of Comenius University and University Hospital, Bratislava, Slovakia.
authors.forename: Helena
authors.initials: H
authors.lastname: Hupková
authors.affiliation: Teaching Department of Burns and Reconstructive Surgery, Medical Faculty of Comenius University and University Hospital, Bratislava, Slovakia.
authors.forename: Ján
authors.initials: J
authors.lastname: Koller
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002056:Burns / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial / Q000502:physiology*; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology; D004926:Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology; D006722:Honey; D006801:Humans; D007097:Imines; D008826:Microbial Sensitivity Tests; D009583:Nitrofurazone / Q000494:pharmacology; D011206:Povidone-Iodine / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology; D011725:Pyridines / Q000494:pharmacology; D012837:Silver Sulfadiazine / Q000494:pharmacology; D041162:Staphylococcus haemolyticus / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology
chemical_list: D000891:Anti-Infective Agents, Local; D007097:Imines; D011725:Pyridines; D011206:Povidone-Iodine; C034213:octenidine; D019342:Acetic Acid; D012837:Silver Sulfadiazine; D009583:Nitrofurazone
keywords: Bacterial infection; In vitro burn wound model; Topical antimicrobials