title: Nanohybrid structure analysis and biomolecule release behavior of polysaccharide-CDHA drug carriers.
abstract: Nanoscaled polymer composites were prepared from polysaccharide chitosan (CS) and Ca-deficient hydroxyapatite (CDHA). CS-CDHA nanocomposites were synthesized by in situ precipitation at pH 9, and the CS-CDHA carriers were then fabricated by ionic cross-linking methods using tripolyphosphate and chemical cross-linking methods by glutaraldehyde and genipin. Certain biomolecules such as vitamin B12, cytochrome c, and bovine serum albumin were loaded into the CS-CDHA carriers, and their release behaviors were investigated. Furthermore, these CS-CDHA carriers were examined by transmission electron microscopy, electron spectroscopy for chemical analysis, and X-ray diffraction. The release behavior of the biomolecules was controlled by the CS/CDHA ratios and cross-linked agents. By increasing the concentration of CS and the concentration of the cross-linking agents, cross-linking within carriers increases, and the release rate of the biomolecules is decreased. Moreover, the release rate of the biomolecules from the CS-CDHA carriers at pH 4 was higher than that at pH 10, displaying a pH-sensitive behavior. Therefore, these CS-CDHA hydrogel beads may be useful for intelligent drug release and accelerate bone reconstruction.
authors.affiliation: Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan. tyliu0322@gmail.com.
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Huang
authors.forename: Ting-Yu
authors.initials: TY
authors.lastname: Liu
authors.forename: Tse-Ying
authors.initials: TY
authors.lastname: Liu
authors.forename: Andreas
authors.initials: A
authors.lastname: Mevold
authors.forename: Andri
authors.initials: A
authors.lastname: Hardiansyah
authors.forename: Hung-Chou
authors.initials: HC
authors.lastname: Liao
authors.forename: Chin-Ching
authors.initials: CC
authors.lastname: Lin
authors.forename: Ming-Chien
authors.initials: MC
authors.lastname: Yang