title: Expression of fatty acid desaturase genes and fatty acid accumulation in Chlamydomonas sp. ICE-L under salt stress.
abstract: The Antarctic ice microalgae Chlamydomonas sp. ICE-L which is highly resistant to salt stress holds promise in providing an alternative species for the production of microalgal oil. We studied the effects of the alga in confrontation with NaCl stress on the growth, oil yield and expression of fatty acid desaturase genes. The growth rate of Chlamydomonas sp. ICE-L decreased with the gradual increase in NaCl concentration. Interestingly, we found that the highest lipid content was achieved at 16‰ NaCl, reaching 23% (w/w). Meanwhile, the expression of Δ9ACPCiFAD increased rapidly while Δ12CiFAD, ω3CiFAD2 and Δ6CiFAD showed a delayed elevation in response to altered salt stress. C18:3 was the dominant PUFA, which account for about 75% TFA in Chlamydomonas sp. ICE-L. Under 96‰ and 128‰ NaCl stress, the content of C20:5 almost approached that of C18:3. In contrast, low salinity enhanced the dominance of C18:3 at the expense of C20:3 and C20:5.
authors.affiliation: Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, Qingdao, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: An
authors.forename: Shanli
authors.initials: S
authors.lastname: Mou
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhou
authors.initials: Z
authors.lastname: Zheng
authors.forename: Naihao
authors.initials: N
authors.lastname: Ye
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jinlai
authors.initials: J
authors.lastname: Miao
mesh_terms: D018533:Biomass; D002696:Chlamydomonas / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D002734:Chlorophyll / Q000378:metabolism; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D005453:Fluorescence; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics*; D008055:Lipids / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D054712:Salinity; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects* / Q000235:genetics
chemical_list: D005227:Fatty Acids; D008055:Lipids; D002734:Chlorophyll; D012965:Sodium Chloride; D044943:Fatty Acid Desaturases
keywords: Chlamydomonas sp. ICE-L; FADs; PUFAs; Salt stress