title: Resveratrol mainly stimulates the glycolytic ATP synthesis flux and not the mitochondrial one: a saturation transfer NMR study in perfused and isolated rat liver.
abstract: Our aim was to monitor the effects of resveratrol (RSV) on the respective contribution of glycolysis and oxidative phosphorylation on the unidirectional flux of ATP synthesis in whole isolated rat liver perfused with Krebs-Henseleit Buffer (KHB). The rate of tissular ATP supply was measured directly by monitoring the chemical exchange Pi toward ATP with saturation transfer (ST) (31)P nuclear magnetic resonance, a method applied for the first time for studying the effects of RSV. ST allows the measurement of the total cellular Pi→ATP chemical exchange; after specific inhibition of glycolysis with iodacetate, ST could provide the Pi→ATP flux issued from mitochondria. This latter was compared to mitochondrial ATP turn-over evaluated after chemical ischemia (CI), performed with specific inhibition (KCN) of oxidative phosphorylation, and measured by standard (31)P NMR spectroscopy. In controls (KHB alone), the apparent time constant (ks) of Pi exchange toward ATP as measured by ST was 0.48±0.04s(-1) leading to a total ATP synthesis rate of 37±3.9μmolmin(-1)g(-1). KHB+RSV perfusion increased ks (+52%; p=0.0009 vs. KHB) leading to an enhanced rate of total ATP synthesis (+52%; p=0.01 vs. KHB). When glycolysis was previously inhibited in KHB, both ks and ATP synthesis flux dramatically decreased (-87% and -86%, respectively, p<0.0001 vs. KHB without inhibition), evidencing a collapse of Pi-to-ATP exchange. However, glycolysis inhibition in KHB+RSV reduced to less extent ks (-41%, p=0.0005 vs. KHB+RSV without inhibition) and ATP synthesis flux (-18%). Using the CI method in KHB and KHB+RSV, KCN addition after glycolysis inhibition induced a rapid fall to zero of the ATP content. The mitochondrial ATP turnover R(t0) and its time constant kd mito were similar in KHB (1.18±0.19μmolmin(-1)g(-1) and 0.91±0.13min(-1)) and KHB+RSV (1.36±0.26μmolmin(-1)g(-1) and 0.77±0.18min(-1)). Since mitochondrial ATP turnover was not increased by RSV, the stimulation of Pi-to-ATP exchange by RSV mainly reflected an increase in glycolytic ATP synthesis flux. Moreover, the maintenance by RSV of a high level of Pi-to-ATP exchange after glycolysis inhibition evidenced a protective effect of the polyphenol, in agreement with our previous hypothesis of a stimulation of substrate flux throughout the glycolysis 3-carbon step.
authors.affiliation: Centre de Résonance Magnétique des Systèmes Biologiques, UMR 5536 CNRS-Université, Bordeaux Segalen, LabEx TRAIL-IBIO, 146 rue Léo Saignat, F-33076 Bordeaux Cedex, France. Electronic address: marie-christine.beauvieux@chu-bordeaux.fr.
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Beauvieux
authors.forename: Alan
authors.initials: A
authors.lastname: Stephant
authors.forename: Henri
authors.initials: H
authors.lastname: Gin
authors.forename: Nizar
authors.initials: N
authors.lastname: Serhan
authors.forename: Patrice
authors.initials: P
authors.lastname: Couzigou
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Gallis
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D006019:Glycolysis / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D013267:Stilbenes; D000255:Adenosine Triphosphate; D000077185:Resveratrol
keywords: AMPK; AMPkinase; ATP; CI; G3PDH; Glycolysis; IAA; Iodoacetate (PubChem CID: 5240); KHB; Krebs-Henseleit Buffer; NMR; PFK; Pi; Potassium cyanide KCN (PubChem CID: 9032); R; RSV; Resveratrol; SIRT1; ST; Saturation transfer NMR; TCA; Trans-resveratrol (PubChem CID: 445154); Vs; apparent catalytic time constant; apparent synthesis time constant; chemical ischemia; glyceraldehyde-3-phosphate-dehydrogenase; inorganic phosphate; iodacetate; k(d mito); k(s); nuclear magnetic resonance; phosphofructokinase; rate of ATP synthesis by ST; rate of mitochondrial ATP synthesis; saturation transfer; sirtuin 1; trans-resveratrol; tricarboxylic cycle; wet weight; ww