title: Application of a new diagnostic approach to a bovine genital campylobacteriosis outbreak in a Saskatchewan beef herd.
abstract: A new real-time quantitative polymerase chain reaction (qPCR) test was used to diagnose Campylobacter fetus subsp. venerealis infection associated with dramatic reproductive losses in a commercial cow-calf herd. The results were verified with repeated culture, phenotypic characterization of the organism and DNA sequencing. This case demonstrates the need for a practical field test for C. fetus subsp. venerealis and the importance of considering this organism as a potential cause of pregnancy failure in beef herds.
authors.affiliation: Department of Large Animal Clinical Sciences (Waldner, Hendrick, Garcia Guerra, Campbell), Department of Veterinary Microbiology (Chaban, Hill), Western College of Veterinary Medicine, Saskatoon, Saskatchewan S7N 5B4; Southwest Animal Health Center, Swift Current, Saskatchewan S9H 4G6 (Griffin).
authors.forename: Cheryl
authors.initials: C
authors.lastname: Waldner
authors.forename: Steve
authors.initials: S
authors.lastname: Hendrick
authors.forename: Bonnie
authors.initials: B
authors.lastname: Chaban
authors.forename: Alvaro
authors.initials: A
authors.lastname: Garcia Guerra
authors.forename: Glen
authors.initials: G
authors.lastname: Griffin
authors.forename: John
authors.initials: J
authors.lastname: Campbell
authors.forename: Janet E
authors.initials: JE
authors.lastname: Hill
mesh_terms: D000818:Animals; D002169:Campylobacter Infections / Q000453:epidemiology / Q000662:veterinary*; D002168:Campylobacter fetus / Q000145:classification / Q000302:isolation & purification*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D004196:Disease Outbreaks / Q000662:veterinary*; D005260:Female; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D012525:Saskatchewan / Q000453:epidemiology; D015231:Sexually Transmitted Diseases, Bacterial / Q000453:epidemiology / Q000662:veterinary*