title: Lymphangiogenic and angiogenic microvessel density in chinese patients with gastric carcinoma: correlation with clinicopathologic parameters and prognosis.
abstract: The incidence of gastric cancer worldwide, and in particular in developing countries, has shown a marked increase. Poor prognosis of gastric cancer patients occurs due to the rapid metastasis of the disease via the lymphatic and blood vessels. The aim of this study was to investigate the expression and the clinical significance of D2-40 and CD34 in human gastric cancer. D2-40 and CD34 expression wasdetected in 1,072 cases of Chinese patients with gastric carcinoma using immunohistochemistry. The lymphatic vessel density (LVD) and microvessel density (MVD) were calculated and analyzed and the correlation with the clinicopathological factors and prognosis was determined. The LVD and MVD of the gastric cancer cases were significantly higher compared to those of normal tissues (P < 0.05). The expression of D2-40-LVD and CD34-MVD in the malignancies were positively related to the age, tumor size, invasion depth, lymphatic metastasis and pathological tumor-node-metastasis (pTNM) (P < 0.05); However, no statistically significant difference was identified between them with the patient gender (P > 0.05). Up-regulation of D2-40 and CD34 expression was significantly correlated with the poor survival rate in univariate and multivariate analyses. The LVD marked by D2-40 and the MVD marked by CD34 were positively correlated to the clinicopathological factors of the malignancies and may play important role in the development and progression of gastric cancer.
authors.affiliation: Department of Surgery, the Affiliated Kunshan First People's Hospital, Jiangsu University, Kunshan, China E-mail : dinghouzhong@hotmail.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Cao
authors.forename: Yong-Wei
authors.initials: YW
authors.lastname: Hu
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Kuo
authors.initials: K
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Li
authors.forename: Can-Rong
authors.initials: CR
authors.lastname: Ni
authors.forename: Hou-Zhong
authors.initials: HZ
authors.lastname: Ding
mesh_terms: D058846:Antibodies, Monoclonal, Murine-Derived / Q000276:immunology / Q000378:metabolism*; D018952:Antigens, CD34 / Q000276:immunology / Q000378:metabolism*; D044466:Asian People; D014408:Biomarkers, Tumor / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D042583:Lymphangiogenesis; D042601:Lymphatic Vessels / Q000378:metabolism / Q000473:pathology*; D008297:Male; D055806:Microvessels / Q000378:metabolism / Q000473:pathology*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology*; D011379:Prognosis; D013274:Stomach Neoplasms / Q000098:blood supply / Q000378:metabolism / Q000473:pathology*; D015996:Survival Rate
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D018952:Antigens, CD34; D014408:Biomarkers, Tumor; C500987:monoclonal antibody D2-40