title: Multinucleation and cell dysfunction induced by amorphous silica nanoparticles in an L-02 human hepatic cell line.
abstract: Silica nanoparticles (SNPs) are one of the most important nanomaterials, and have been widely used in a variety of fields. Therefore, their effects on human health and the environment have been addressed in a number of studies. In this work, the effects of amorphous SNPs were investigated with regard to multinucleation in L-02 human hepatic cells. Our results show that L-02 cells had an abnormally high incidence of multinucleation upon exposure to silica, that increased in a dose-dependent manner. Propidium iodide staining showed that multinucleated cells were arrested in G2/M phase of the cell cycle. Increased multinucleation in L-02 cells was associated with increased generation of cellular reactive oxygen species and mitochondrial damage on flow cytometry and confocal microscopy, which might have led to failure of cytokinesis in these cells. Further, SNPs inhibited cell growth and induced apoptosis in exposed cells. Taken together, our findings demonstrate that multinucleation in L-02 human hepatic cells might be a failure to undergo cytokinesis or cell fusion in response to SNPs, and the increase in cellular reactive oxygen species could be responsible for the apoptosis seen in both mononuclear cells and multinucleated cells.
authors.affiliation: School of Public Health, Capital Medical University, Beijing ; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing ; School of Public Health, Jilin University, Changchun, Jilin.
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Liu
authors.forename: Minghua
authors.initials: M
authors.lastname: Jin
authors.forename: Haiying
authors.initials: H
authors.lastname: Du
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Peili
authors.initials: P
authors.lastname: Huang
authors.forename: Xianqing
authors.initials: X
authors.lastname: Zhou
authors.forename: Lan
authors.initials: L
authors.lastname: Yuan
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Sun
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology* / Q000502:physiology*; D006801:Humans; D053768:Metal Nanoparticles / Q000009:adverse effects*; D010316:Particle Size; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012834:Silver / Q000009:adverse effects*
chemical_list: D017382:Reactive Oxygen Species; D012834:Silver
keywords: apoptosis; cell cycle; cell dysfunction; human hepatic cell L-02; multinucleation; silica nanoparticles