title: Simultaneous determination of seven constituents in herbal prescription jaeumganghwa-tang using HPLC-PDA.
abstract: A simple and accurate high-performance liquid chromatographic method was applied to the quantitative analysis of seven components of the traditional herbal prescription Jaeumganghwa-tang (JGT), including 5-hydroxymethyl-2-furaldehyde, albiflorin, paeoniflorin, liquiritin, ferulic acid, nodakenin, and glycyrrhizin. All seven compounds were separated in less than 40 min on a Gemini C18 column at 40°C by gradient elution using 1.0% (v/v) aqueous acetic acid and acetonitrile containing 1.0% (v/v) acetic acid as mobile phase. The flow rate was 1.0 mL/min and the detector was a photodiode array (PDA) set at 230 nm, 254 nm, 280 nm, and 330 nm. The calibration curves showed good linearity (r (2) > 0.9998) in different concentration ranges. The recovery of each component was in the range of 91.47-102.62%, with relative standard deviations (RSDs, %) less than 4.5%. The RSDs (%) for intra- and interday precision were 0.06-2.85% and 0.06-2.83%, respectively. The concentrations of the seven components in JGT were in the range 0.74-5.48 mg/g.
authors.affiliation: Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon, 305-811, Korea.
authors.forename: Chang-Seob
authors.initials: CS
authors.lastname: Seo
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Hyeun-Kyoo
authors.initials: HK
authors.lastname: Shin
mesh_terms: D001565:Benzoates / Q000032:analysis; D001952:Bridged-Ring Compounds / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003373:Coumaric Acids / Q000032:analysis; D003374:Coumarins / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D044950:Flavanones / Q000032:analysis; D005662:Furaldehyde / Q000031:analogs & derivatives / Q000032:analysis; D005960:Glucosides / Q000032:analysis; D019695:Glycyrrhizic Acid / Q000032:analysis; D019684:Magnoliopsida / Q000737:chemistry*; D039821:Monoterpenes; D006027:Glycosides
chemical_list: D001565:Benzoates; D001952:Bridged-Ring Compounds; D003373:Coumaric Acids; D003374:Coumarins; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005662:Furaldehyde; D005960:Glucosides; D019695:Glycyrrhizic Acid; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides; C014959:albiflorin; C008046:5-hydroxymethylfurfural; C004999:ferulic acid; C471579:nodakenin; C512196:liquiritin
keywords: HPLC-PDA; Herbal prescription; Jaeumganghwa-tang; Jiin-koka-to; Simultaneous determination; Zi-yin-jiang-huo-tang