title: Plasma and EBC microRNAs as early biomarkers of non-small-cell lung cancer.
abstract: Lung cancer is a major cause of death in Western countries. Current screening methods are invasive and still lead to a high percentage of false positives. There is, therefore, a need to find biomarkers that increase the probability of detecting lung cancer early. MicroRNAs (miRNAs) are stable molecules in blood plasma and exhaled breath condensate (EBC). We quantified miRNA-21 and miRNA-486 expression from plasma and EBC samples from patients with a diagnosis of non-small-cell lung cancer (NSCLC) and controls. miRNA-21 was significantly higher in plasma and in EBC of the NSCLC patients and miRNA-486 was significantly lower. This difference indicates a significantly improved diagnostic value, and suggests that these miRNAs could be clinically used as a first-line screening test in high-risk subjects.
authors.affiliation: Department of Clinical and Experimental Medicine, University of Parma , Parma , Italy .
authors.forename: Paola
authors.initials: P
authors.lastname: Mozzoni
authors.forename: Iris
authors.initials: I
authors.lastname: Banda
authors.forename: Matteo
authors.initials: M
authors.lastname: Goldoni
authors.forename: Massimo
authors.initials: M
authors.lastname: Corradi
authors.forename: Marcello
authors.initials: M
authors.lastname: Tiseo
authors.forename: Olga
authors.initials: O
authors.lastname: Acampa
authors.forename: Valeria
authors.initials: V
authors.lastname: Balestra
authors.forename: Luca
authors.initials: L
authors.lastname: Ampollini
authors.forename: Angelo
authors.initials: A
authors.lastname: Casalini
authors.forename: Paolo
authors.initials: P
authors.lastname: Carbognani
authors.forename: Antonio
authors.initials: A
authors.lastname: Mutti
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood*; D001944:Breath Tests; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood*; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015415:Biomarkers; C524940:MIRN21 microRNA, human; C560945:MIRN486 microRNA, human; D035683:MicroRNAs