title: Development and prospects of organ replacement regenerative therapy.
abstract: Current approaches for the development of regenerative therapies have been influenced by our understanding of embryonic development, stem cell biology, and tissue engineering technology. The ultimate goal of regenerative therapy is to develop fully functioning bioengineered organs to replace lost or damaged organs that result from disease, injury, or aging. Almost all organs including ectodermal organs, such as teeth, hair, salivary glands, and lacrimal glands, arise from organ germs induced by reciprocal epithelial-mesenchymal interactions in the developing embryo. A novel concept to generate a bioengineered organ is to recreate organogenesis and thereby develop fully functioning bioengineered organs from the resulting bioengineered organ germ generated via 3-dimensional cell manipulation using immature stem cells in vitro. We have previously developed a bioengineering method for forming a 3-dimensional organ germ in the early developmental stages, termed the "bioengineered organ germ method." Recently, we reported fully functioning bioengineered tooth replacements after transplantation of a bioengineered tooth germ or a mature tooth unit comprising the bioengineered tooth and periodontal tissues. This concept could be adopted to generate not only teeth but also bioengineered hair follicles, salivary glands, and lacrimal glands. These studies emphasize the potential for bioengineered organ replacement in future regenerative therapies. In this review, we will summarize the strategies and the recent progress of research and development for the establishment of organ replacement regenerative therapies.
authors.affiliation: *Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan; †Research Institute for Science and Technology, Tokyo University of Science, Chiba, Japan; ‡Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Chiba, Japan; and §Organ Technologies, Inc, Chiyoda-ku, Tokyo, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Hirayama
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Oshima
authors.forename: Takashi
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D021522:Bioartificial Organs; D001698:Biomedical Engineering / Q000379:methods*; D006801:Humans; D009924:Organ Culture Techniques / Q000379:methods*; D038081:Organogenesis / Q000502:physiology; D013234:Stem Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*