title: High-throughput genotyping system as a robust and useful tool in oncology: experience from a single institution.
abstract: OBJECTIVE
  Single nucleotide polymorphisms (SNPs) are substitutions of one base for another in the gene sequence and conforms the basis for pharmacogenetics and the development of personalized medicine. Many methods have been developed for SNP genotyping. The aim of the present study was to validate the use of a novel high-throughput genotyping system.
  
  
  METHODS
  Five SNPs (rs25487, rs25489, rs1799782, rs13181, and rs11615) were genotyped in 118 cancer patients using the classical method PCR restriction fragment length polymorphism (RFLP) and the high-throughput, automated assay Biotrove OpenArray(®) NT Cycler, trying to explore the feasibility and reproducibility of the OpenArray system in the context of oncology.
  
  
  RESULTS
  The call rates obtained ranged from 95.7 to 100% for both techniques. The percentage of overlapping ranged from 96.2 to 100% among both assays, showing a high reproducibility between the techniques.
  
  
  CONCLUSIONS
  These findings, together with the low-cost and the simple and fast work flow, suggest that the OpenArray system is a robust and easy methodology for genotyping in the field of oncology.
authors.affiliation: Radiation Oncology Department, Hospital Universitario de Gran Canaria, Dr. Negrín, C/Barranco de La Ballena s/n, CP 35010, Las Palmas de Gran Canaria, Spain; Instituto Canario de Investigación del Cáncer, Las Palmas de Gran Canaria, Spain; Clinical Sciences Department, Universidad de Las Palmas de Gran Canaria, C/Dr. Pasteur s/n, CP 35016, Las Palmas de Gran Canaria, Spain. Electronic address: lhenriquez@dcc.ulpgc.es.
authors.forename: Luis Alberto
authors.initials: LA
authors.lastname: Henríquez-Hernández
authors.forename: Almudena
authors.initials: A
authors.lastname: Valenciano
authors.forename: Estefanía
authors.initials: E
authors.lastname: Herrera-Ramos
authors.forename: Marta
authors.initials: M
authors.lastname: Lloret
authors.forename: Alba
authors.initials: A
authors.lastname: Riveros-Perez
authors.forename: Pedro C
authors.initials: PC
authors.lastname: Lara
mesh_terms: D005240:Feasibility Studies; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D046228:Microarray Analysis; D009369:Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000628:therapy; D017063:Outcome Assessment, Health Care / Q000379:methods; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results
keywords: Medical diagnostics technology; Microarray analysis; OpenArray; PCR-RFLP; SNP assay