title: Biosynthetic incorporation of tryptophan analogs in proteins.
abstract: Biosynthetic incorporation of Trp analogs in a protein can help in its characterization using fluorescence spectroscopy and other methodologies like NMR and phosphorescence. Here a protocol is presented resulting in the efficient incorporation of Trp analogs in a recombinant protein, using an Escherichia coli Trp auxotroph. An overview of recent developments in the Trp analog incorporation field is also presented.
authors.affiliation: Laboratory of Biophysical Chemistry and Groningen Biomolecular Science and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.
authors.forename: Jaap
authors.initials: J
authors.lastname: Broos
mesh_terms: D004926:Escherichia coli; D009682:Magnetic Resonance Spectroscopy; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000031:analogs & derivatives / Q000737:chemistry*
chemical_list: D011994:Recombinant Proteins; D014364:Tryptophan