title: The (13)C-butyrate breath test: a new non-invasive method for assessing colitis in a murine model.
abstract: The conventional method for the real-time assessment of murine colitis requires a large number of animals. The (13)C-butyrate breath test could be useful for evaluating disease activity and the amelioration of human ulcerative colitis non-invasively. The purpose of this study was to investigate whether this test can be used to assess the phase of inflammation in murine colitis. We investigated the excretion of (13)CO2 measured by the (13)C-butyrate breath test after rectal instillation of butyrate in the DSS colitis model. The colon length, MPO activity, and histological damage were analyzed as parameters. The efficacy of salicylazosulfa-pyridine (SASP) on (13)CO2 excretion was also studied. The (13)CO2 excretion curves in the 0.5% DSS- and 0.75% DSS-treated groups were significantly lower than those in the normal group (P < 0.01, P < 0.01). Good correlation between the results of the breath test and the inflammation parameters was observed. The (13)CO2 excretion curve in DSS murine colitis after the administration of SASP was significantly higher than in the normal group (P < 0.01). The (13)C-butyrate breath test can be used to evaluate the inflammatory phase of DSS murine colitis, and it may be a new non-invasive method for assessing murine colitis.
authors.affiliation: Department of Clinical Research, Oriental Medicine Research Center, Kitasato University, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Endo
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Oikawa
authors.forename: Takayuki
authors.initials: T
authors.lastname: Hoshino
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Hatori
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Hanawa
mesh_terms: D000285:Administration, Rectal; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001944:Breath Tests / Q000379:methods*; D002087:Butyrates / Q000008:administration & dosage; D002245:Carbon Dioxide / Q000032:analysis; D002247:Carbon Isotopes / Q000032:analysis; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000175:diagnosis* / Q000188:drug therapy / Q000473:pathology; D003106:Colon / Q000201:enzymology / Q000473:pathology; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D009149:Mustard Plant; D009195:Peroxidase / Q000378:metabolism; D010938:Plant Oils; D012460:Sulfasalazine / Q000031:analogs & derivatives / Q000627:therapeutic use
chemical_list: D015415:Biomarkers; D002087:Butyrates; D002247:Carbon Isotopes; D010938:Plant Oils; D002245:Carbon Dioxide; D012460:Sulfasalazine; D016264:Dextran Sulfate; D009195:Peroxidase; C016095:salicylazoiminopyridine; C027793:mustard oil