title: Label-free electrochemical biosensor using home-made 10-methyl-3-nitro-acridone as indicator for picomolar detection of nuclear factor kappa B.
abstract: A new acridone derivative 10-methyl-3-nitro-acridone (MNA) with excellent electrochemical activity was synthesized in this paper. Using it as an electrochemical indicator, a signal-on and label-free electrochemical biosensor was developed for picomolar determination of nuclear factor kappa B (NF-κB) in serum. Initially, linear double-stranded DNA (dsDNA) probes, which contain a protein-binding site specific to NF-κB, were self-assembled on the surface of a glass carbon electrode (GCE). If the NF-κB was absent, the dsDNA probes were cut into ss-DNA fragments by the digestion of ExoIII, resulting in a low electrochemical signal of MNA due to the weak binding affinity of MNA to ss-DNA. On the contrary, in the presence of NF-κB, it could bind with the dsDNA probes at the specific site and hinder the digestion of ExoIII, resulting in a significant increase of electrochemical response due to the intercalation of MNA into the dsDNA probes. By employing the above strategy, this sensor could detect as low as 40 pM NF-κB with high specificity. To the best of our knowledge, the proposed sensor is the first attempt to use acridone derivative as an electrochemical indicator for NF-κB detection, which may represent a promising path toward clinical diagnosis and drug developments.
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350004, China. Electronic address: cjh_huaxue@126.com.
authors.forename: Jing Hua
authors.initials: JH
authors.lastname: Chen
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.forename: Shuxian
authors.initials: S
authors.lastname: Cai
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Wu
authors.forename: Jia
authors.initials: J
authors.lastname: Lin
authors.forename: Chunyan
authors.initials: C
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D054831:Acridones / Q000138:chemical synthesis* / Q000737:chemistry; D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D006801:Humans; D016328:NF-kappa B / Q000737:chemistry / Q000302:isolation & purification*; D011485:Protein Binding
chemical_list: D054831:Acridones; D016328:NF-kappa B; D002244:Carbon; D004247:DNA
keywords: 10-methyl-3-nitro-acridone; Electrochemical biosensor; Nuclear factor kappa B