title: Measurement of subcutaneous biological substances using thin metal needle with micro flow channel.
abstract: Concentrations of biological substances are useful as indicators of physiological and pathological states. In order to monitor biological substances in daily life, we developed a minimally invasive needle type device with which biological substances are extracted through a microperfusion system inserted under the skin. The perfusion needle has a flow channel with perforated membrane through which biological substances from subepidermal tissue are extracted. The efficacy of the device was examined by measuring lactate concentration of exercising mice. Lactate was successfully collected from the back skin of the mice running on a treadmill using a fabricated microperfusion needle. Lactate concentration of perfused solution correlated with blood lactate concentration.
authors.forename: N
authors.initials: N
authors.lastname: Tsuruoka
authors.forename: K
authors.initials: K
authors.lastname: Ishii
authors.forename: T
authors.initials: T
authors.lastname: Matsunaga
authors.forename: R
authors.initials: R
authors.lastname: Nagatomi
authors.forename: Y
authors.initials: Y
authors.lastname: Haga
mesh_terms: D000818:Animals; D004867:Equipment Design; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D008670:Metals; D051379:Mice; D008810:Mice, Inbred C57BL; D008991:Monitoring, Physiologic / Q000379:methods*; D009339:Needles; D010477:Perfusion; D010805:Physical Conditioning, Animal; D012867:Skin / Q000473:pathology*
chemical_list: D008670:Metals; D019344:Lactic Acid