title: Thromboelastography platelet mapping in healthy dogs using 1 analyzer versus 2 analyzers.
abstract: The objective of this study was to describe the results of thromboelastography platelet mapping (TEG-PM) carried out using 2 techniques in 20 healthy dogs. Maximum amplitudes (MA) generated by thrombin (MAthrombin), fibrin (MAfibrin), adenosine diphosphate (ADP) receptor activity (MAADP), and thromboxane A2 (TxA2) receptor activity (stimulated by arachidonic acid, MAAA) were recorded. Thromboelastography platelet mapping was carried out according to the manufacturer's guidelines (2-analyzer technique) and using a variation of this method employing only 1 analyzer (1-analyzer technique) on 2 separate blood samples obtained from each dog. Mean [± standard deviation (SD)] MA values for the 1-analyzer/2-analyzer techniques were: MAthrombin = 51.9 mm (± 7.1)/52.5 mm (± 8.0); MAfibrin = 20.7 mm (± 21.8)/23.0 mm (± 26.1); MAADP = 44.5 mm (± 15.6)/45.6 mm (± 17.0); and MAAA = 45.7 mm (± 11.6)/45.0 mm (± 15.4). Mean (± SD) percentage aggregation due to ADP receptor activity was 70.4% (± 32.8)/67.6% (± 33.7). Mean percentage aggregation due to TxA2 receptor activity was 77.3% (± 31.6)/78.1% (± 50.2). Results of TEG-PM were not significantly different for the 1-analyzer and 2-analyzer methods. High correlation was found between the 2 methods for MAfibrin [concordance correlation coefficient (r) = 0.930]; moderate correlation was found for MAthrombin (r = 0.70) and MAADP (r = 0.57); correlation between the 2 methods for MAAA was lower (r = 0.32). Thromboelastography platelet mapping (TEG-PM) should be further investigated to determine if it is a suitable method for measuring platelet dysfunction in dogs with thrombopathy.
authors.affiliation: Department of Clinical Studies (Blois, Banerjee, Park) and Department of Pathobiology (Wood), Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1.
authors.forename: Shauna L
authors.initials: SL
authors.lastname: Blois
authors.forename: Amrita
authors.initials: A
authors.lastname: Banerjee
authors.forename: R Darren
authors.initials: RD
authors.lastname: Wood
authors.forename: Fiona M
authors.initials: FM
authors.lastname: Park
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D004285:Dogs / Q000502:physiology*; D005260:Female; D005337:Fibrin / Q000494:pharmacology; D008297:Male; D010974:Platelet Aggregation / Q000502:physiology*; D018709:Statistics, Nonparametric; D013916:Thrombelastography / Q000379:methods / Q000662:veterinary*; D013917:Thrombin / Q000494:pharmacology; D013928:Thromboxane A2 / Q000494:pharmacology
chemical_list: D013928:Thromboxane A2; D000244:Adenosine Diphosphate; D005337:Fibrin; D013917:Thrombin