title: Consistency of influenza A virus detection test results across respiratory specimen collection methods using real-time reverse transcription-PCR.
abstract: In our prospective cohort study, we compared the performance of nasopharyngeal, oropharyngeal, and nasal swabs for the detection of influenza virus using real-time reverse transcription-PCR assay. Joint consideration of results from oropharyngeal and nasal swabs was as effective as consideration of results from nasopharyngeal swabs alone, as measured by sensitivity and noninferiority analysis.
authors.affiliation: Epidemiology and Prevention Branch, Influenza Division, National Center for Immunization and Respiratory Diseases (NCIRD), Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: Spencer
authors.forename: Manjusha
authors.initials: M
authors.lastname: Gaglani
authors.forename: Allison
authors.initials: A
authors.lastname: Naleway
authors.forename: Sue
authors.initials: S
authors.lastname: Reynolds
authors.forename: Sarah
authors.initials: S
authors.lastname: Ball
authors.forename: Sam
authors.initials: S
authors.lastname: Bozeman
authors.forename: Emily
authors.initials: E
authors.lastname: Henkle
authors.forename: Jennifer
authors.initials: J
authors.lastname: Meece
authors.forename: Mary
authors.initials: M
authors.lastname: Vandermause
authors.forename: Lydia
authors.initials: L
authors.lastname: Clipper
authors.forename: Mark
authors.initials: M
authors.lastname: Thompson
mesh_terms: D000328:Adult; D015331:Cohort Studies; D005260:Female; D006801:Humans; D009980:Influenza A virus / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis*; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009296:Nasal Cavity / Q000821:virology; D009305:Nasopharynx / Q000821:virology; D009960:Oropharynx / Q000821:virology; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*