title: From a gene-centric to whole-proteome view of differentiation of T helper cell subsets.
abstract: Proper differentiation of naïve T helper cells into functionally distinct subsets is of critical importance to human health. Consequently, the process is tightly controlled by a complex intracellular signalling network. To dissect the regulatory principles of this network, immunologists have early on embraced system-wide transcriptomics tools, leading to identification of large panels of potential regulatory factors. In contrast, the use of proteomics approaches in T helper cell research has been notably rare, and to this date relatively few high-throughput datasets have been reported. Here, we discuss the importance of such research and envision the possibilities afforded by mass spectrometry-based proteomics in the near future.
authors.affiliation: European Molecular Biology Laboratory European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, United Kingdom. tapio.lonnberg@ebi.ac.uk.
authors.forename: Tapio
authors.initials: T
authors.lastname: Lönnberg
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.forename: Riitta
authors.initials: R
authors.lastname: Lahesmaa
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D006801:Humans; D013058:Mass Spectrometry; D020543:Proteome / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology* / Q000378:metabolism*
chemical_list: D020543:Proteome
keywords: T cell activation; T helper cell; mass spectrometry; proteomics; systems biology; transcriptomics