title: Selenium deficiency is associated with endoplasmic reticulum stress in a rat model of cardiac malfunction.
abstract: The relationship between selenium (Se) deficiency-induced cardiac malfunction and endoplasmic reticulum (ER) stress is poorly understood. In the present study, 18 weaning Sprague Dawley rats were randomly fed with three different Se diets, and myocardial glutathione peroxidase (GPx) activity was measured by an enzyme activity assay. Cardiac function was evaluated by hemodynamic parameters. ER stress markers immunoglobulin-binding protein (BiP)/glucose-regulated protein 78 (GRP78) and C/EBP homologous protein (CHOP) were detected by western blotting. Our data showed that myocardial GPx activity and cardiac function were conspicuously impaired in Se-deficient rats. Expression of GRP78 and CHOP was significantly upregulated by treatment of Se deficiency. Improvements in myocardial GPx activity and cardiac function, as well as decreases in expression of GRP78 and CHOP, were observed after Se supplementation. Consequently, our data show that ER stress was involved in Se deficiency-induced cardiac dysfunction.
authors.affiliation: Department of Cardiology, Second Affiliated Hospital, Xi'an Jiaotong University, 157 Road Xiwu, Xi'an, Shaanxi, 710004, People's Republic of China.
authors.forename: Su-Qin
authors.initials: SQ
authors.lastname: Wang
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Niu
authors.forename: Zhong-Wei
authors.initials: ZW
authors.lastname: Liu
authors.forename: Yan-He
authors.initials: YH
authors.lastname: Zhu
authors.forename: Deng-Feng
authors.initials: DF
authors.lastname: Gao
mesh_terms: D000818:Animals; D019587:Dietary Supplements; D004195:Disease Models, Animal; D059865:Endoplasmic Reticulum Stress; D005786:Gene Expression Regulation / Q000187:drug effects; D006331:Heart Diseases / Q000178:diet therapy / Q000378:metabolism* / Q000473:pathology; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000172:deficiency* / Q000494:pharmacology; D051742:Transcription Factor CHOP / Q000096:biosynthesis; D000091342:Endoplasmic Reticulum Chaperone BiP
chemical_list: D006360:Heat-Shock Proteins; D012643:Selenium; D051742:Transcription Factor CHOP; C497251:Ddit3 protein, rat; C051684:GRP78 protein, rat; D000091342:Endoplasmic Reticulum Chaperone BiP