title: Generation of a monoclonal antibody to S1 protein of porcine epidemic diarrhea virus.
abstract: Gene encoding the N-terminal half of spike protein (S1) of porcine epidemic diarrhea virus (PEDV) was cloned and expressed as a recombinant protein in Escherichia coli BL21 (DE3). Then, female BALB/c mice were immunized with the purified recombinant S1 protein (rS1), and a monoclonal antibody (MAb designated as 5E12) against the rS1 protein was achieved by hybridoma technique. MAb 5E12 not only reacted with rS1 protein indirect ELISA and Western blot, but also recognized PEDV transiently expressed in Vero E6 cells in indirect immunofluorescence examinations. This work suggests that 5E12 would be a useful tool as a specific diagnostic reagent for detecting PEDV S protein.
authors.affiliation: 1 State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Disease of Grazing Animal of Ministry of Agriculture, Lanzhou Veterinary Research Institute , Chinese Academy of Agricultural Science (LVRI, CAAS), Lanzhou, Gansu, China .
authors.forename: Liyan
authors.initials: L
authors.lastname: Cao
authors.forename: Zhaoheng
authors.initials: Z
authors.lastname: Qin
authors.forename: Xuying
authors.initials: X
authors.lastname: Ge
authors.forename: Xiangping
authors.initials: X
authors.lastname: Yin
authors.forename: Cheng
authors.initials: C
authors.lastname: Xia
authors.forename: Ri-e
authors.initials: RE
authors.lastname: Bu
authors.forename: Ying
authors.initials: Y
authors.lastname: Fang
authors.forename: Jixing
authors.initials: J
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Gao
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ren
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D015153:Blotting, Western; D002522:Chlorocebus aethiops; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D053485:Porcine epidemic diarrhea virus / Q000276:immunology*; D064370:Spike Glycoprotein, Coronavirus / Q000276:immunology*; D014709:Vero Cells
chemical_list: D000911:Antibodies, Monoclonal; D017931:DNA Primers; D064370:Spike Glycoprotein, Coronavirus