title: Preliminary characterization of genipin-cross-linked silk sericin/poly(vinyl alcohol) films as two-dimensional wound dressings for the healing of superficial wounds.
abstract: The genipin-cross-linked silk sericin/poly(vinyl alcohol) (PVA) films were developed aiming to be applied as two-dimensional wound dressings for the treatment of superficial wounds. The effects of genipin cross-linking concentration on the physical and biological properties of the films were investigated. The genipin-cross-linked silk sericin/PVA films showed the increased surface density, tensile strength, and percentage of elongation, but decreased percentage of light transmission, water vapor transmission rate, and water swelling, compared to the non-cross-linked films. This explained that the cross-linking bonds between genipin and silk sericin would reduce the mobility of molecular chains within the films, resulting in the more rigid molecular structure. Silk sericin was released from the genipin-cross-linked films in a sustained manner. In addition, either L929 mouse fibroblast or HaCat keratinocyte cells showed high percentage of viability when cultured on the silk sericin/PVA films cross-linked with 0.075 and 0.1% w/v genipin. The in vivo safety test performed according to ISO 10993-6 confirmed that the genipin-cross-linked silk sericin/PVA films were safe for the medical usages. The efficacy of the films for the treatment of superficial skin wounds will be further investigated in vivo and clinically. The genipin-cross-linked silk sericin/PVA films would be promising choices of two-dimensional wound dressings for the treatment of superficial wounds.
authors.affiliation: Bioactive Resources for Innovative Clinical Applications Research Unit, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok 10330, Thailand ; Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok 10330, Thailand.
authors.forename: Tippawan
authors.initials: T
authors.lastname: Siritientong
authors.forename: Juthamas
authors.initials: J
authors.lastname: Ratanavaraporn
authors.forename: Teerapol
authors.initials: T
authors.lastname: Srichana
authors.forename: Pornanong
authors.initials: P
authors.lastname: Aramwit
mesh_terms: D000818:Animals; D001458:Bandages; D001672:Biocompatible Materials / Q000737:chemistry / Q000627:therapeutic use; D039823:Iridoids / Q000737:chemistry / Q000627:therapeutic use*; D051379:Mice; D011142:Polyvinyl Alcohol / Q000627:therapeutic use; D047030:Sericins / Q000737:chemistry / Q000627:therapeutic use; D047011:Silk / Q000737:chemistry / Q000627:therapeutic use*; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D039823:Iridoids; D047030:Sericins; D047011:Silk; D011142:Polyvinyl Alcohol; C007834:genipin