title: Spectroscopic identification and anti-biofilm properties of polar metabolites from the medicinal plant Helichrysum italicum against Pseudomonas aeruginosa.
abstract: Two new acylated styrylpyrones, one 5-methoxy-1(3H)-isobenzofuranone glucoside and a hydroxymethyl-orcinol derivative, along with sixteen known aromatic metabolites, including lignans, quinic acid derivatives low-molecular weight phenol glucosides, have been isolated from the methanol extract of Helichrysum italicum, a medicinal plant typical of the Mediterranean vegetation. The structures of these compounds have been elucidated on the basis of extensive 2D-NMR spectroscopic analyses, including COSY, TOCSY, HSQC, CIGAR-HMBC, H2BC and HSQC-TOCSY, along with Q-TOF HRMS(2) analysis. Selected compounds were evaluated for their anti-biofilm properties against Pseudomonas aeruginosa.
authors.affiliation: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, via Vivaldi 43, 81100 Caserta, Italy. Electronic address: brigida.dabrosca@unina2.it.
authors.forename: Brigida
authors.initials: B
authors.lastname: D'Abrosca
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Buommino
authors.forename: Grazia
authors.initials: G
authors.lastname: D'Angelo
authors.forename: Lorena
authors.initials: L
authors.lastname: Coretti
authors.forename: Monica
authors.initials: M
authors.lastname: Scognamiglio
authors.forename: Valeria
authors.initials: V
authors.lastname: Severino
authors.forename: Severina
authors.initials: S
authors.lastname: Pacifico
authors.forename: Giovanna
authors.initials: G
authors.lastname: Donnarumma
authors.forename: Antonio
authors.initials: A
authors.lastname: Fiorentino
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D036562:Helichrysum / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D012118:Resorcinols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D005960:Glucosides; D010936:Plant Extracts; D012118:Resorcinols; C005282:orcinol
keywords: Anti-biofilm activity; Helichrysum italicum; Pseudomonas aeruginosa; Spectroscopic analysis; Structural characterization