title: Comparative proteomic analysis of the stolon cold stress response between the C4 perennial grass species Zoysia japonica and Zoysia metrella.
abstract: Zoysiagrass, the most cold-tolerant grass among the warm-season turfgrasses, is often used as a model species for isolating cellular components related to cold stress. To understand the proteomic responses to cold stress in zoysiagrass stolons, we extracted stolon proteins from Zoysiajaponica, cv. Meyer (cold-tolerant) and Z. metrella, cv. Diamond (cold-sensitive), which were grown with or without cold treatment. Approximately 700 proteins were resolved on 2-DE gels, and 70 protein spots were differentially accumulated. We further observed that 45 of the identified proteins participate in 10 metabolic pathways and cellular processes. A significantly greater number of proteins accumulated in the Meyer than in the Diamond and 15 increased proteins were detected only in the Meyer cultivar under cold stress. Furthermore, we propose a cold stress-responsive protein network composed of several different functional components that exhibits a balance between reactive oxygen species (ROS) production and scavenging, accelerated protein biosynthesis and proteolysis, reduced protein folding, enhanced photosynthesis, abundant energy supply and enhanced biosynthesis of carbohydrates and nucleotides. Generally, the cold-tolerant Meyer cultivar showed a greater ROS scavenging ability, more abundant energy supply and increased photosynthesis and protein synthesis than did the cold-sensitive Diamond cultivar, which may partly explain why Meyer is more cold tolerant.
authors.affiliation: Institute of Botany, Jiangsu Province and the Chinese Academy of Sciences, No.1 Qianhu Houcun, Zhongshanmenwai, Nanjing, Jiangsu Province, PR China.
authors.forename: Jiping
authors.initials: J
authors.lastname: Xuan
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Song
authors.forename: Hongxiao
authors.initials: H
authors.lastname: Zhang
authors.forename: Jianxiu
authors.initials: J
authors.lastname: Liu
authors.forename: Zhongren
authors.initials: Z
authors.lastname: Guo
authors.forename: Yuelou
authors.initials: Y
authors.lastname: Hua
mesh_terms: D058639:Cold-Shock Response / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D010788:Photosynthesis / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D006109:Poaceae / Q000235:genetics* / Q000378:metabolism*; D020543:Proteome / Q000235:genetics* / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D017382:Reactive Oxygen Species / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D010940:Plant Proteins; D020543:Proteome; D017382:Reactive Oxygen Species