title: Rapid detection of S. mutans surface antigen I/II using a sensitive monoclonal anti-Ag I/II antibody by ELISA.
abstract: The cell-surface protein antigen I/II (Ag I/II) is expressed in oral streptococci, which are known as the causative agent of a number of diseases including dental caries, endocarditis, gingivitis, and periodontal disease. Consequently, monoclonal antibodies (MAb) capable of recognizing the streptococcal Ag I/II protein could be a useful tool for the diagnosis and cure of these diseases. In this study, a previously generated monoclonal anti-Ag I/II antibody, ckAg I/II, was used to detect a small amount of Streptococcus mutans (S. mutans) surface antigen Ag I/II. The ckAg I/II was proved to be very sensitive and able to detect as little as 1 ng of recombinant Ag I/II protein within 5 min and Ag I/II in saliva within 10 min, as well as native Ag I/II in 20 μL of culture supernatant by ELISA. These results suggest that ckAg I/II can be used as a fast and efficient diagnostic tool to detect Ag I/II.
authors.affiliation: 1 Department of Pediatric Dentistry and Institute of Oral Bioscience, Chonbuk National University , Jeonju-si, Jeollabuk-do, Korea.
authors.forename: Mi-Ah
authors.initials: MA
authors.lastname: Kim
authors.forename: Hyun-Soon
authors.initials: HS
authors.lastname: Jeon
authors.forename: Se-Young
authors.initials: SY
authors.lastname: Shin
authors.forename: Byeong-Ju
authors.initials: BJ
authors.lastname: Baik
authors.forename: Yeon-Mi
authors.initials: YM
authors.lastname: Yang
authors.forename: Kyung-Yeol
authors.initials: KY
authors.lastname: Lee
authors.forename: Jae-Gon
authors.initials: JG
authors.lastname: Kim
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D000954:Antigens, Surface / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D009059:Mouth Diseases / Q000175:diagnosis / Q000382:microbiology*; D011994:Recombinant Proteins / Q000276:immunology; D012463:Saliva / Q000382:microbiology; D013295:Streptococcus mutans / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D011994:Recombinant Proteins