title: In vitro and in vivo antimicrobial properties of silver-containing hydroxyapatite prepared via ultrasonic spray pyrolysis route.
abstract: Hydroxyapatite (HAp), with its high biocompatibility and osteoconductivity, readily absorbs proteins, amino acids and other substances, which in turn favor the adsorption and colonization of bacteria. To prevent bacterial growth and biofilm formation on HAp discs, silver-containing (1-20 mol%) HAp (Ag-HAp) powders were synthesized using an ultrasonic spray pyrolysis (USSP) technique. The X-ray diffraction (XRD) peaks were very broad, indicating low crystallinity, and this induced the release of Ag(+) ions from Ag-HAp powders. In addition, a gradual increase in Ca(2+) ion release was observed. These results suggest that dissolution of Ca(2+) ion in Ag-HAp triggered the release of Ag(+) ions. The antimicrobial efficacy of Ag-HAp disc was tested against Staphylococcus aureus. Samples with Ag contents of more than 5 mol% were found to be highly effective against bacterial colonization and biofilm formation in vitro. In vivo antibacterial tests using bioluminescent strains also showed reductions in the viability of bacteria with Ag-HAp (5 mol%) discs. Biocompatibility tests using a modified Transwell® insert method showed that Ag-HAp (5 mol%) discs have negative effects on osteoblast proliferation. These results indicate that Ag-HAp (5 mol%) has effective antibacterial activity and good biocompatibility both in vitro and in vivo together with good biocompatibility, thus confirming its utility as a bactericidal material.
authors.affiliation: Aizawa "Next-generation Bioceramics" Project, Kanagawa Academy of Science and Technology (KAST), 3-2-1 Sakado, Takatsu-ku, Kawasaki, Kanagawa 213-0012, Japan. Electronic address: ap-honda@newkast.or.jp.
authors.forename: Michiyo
authors.initials: M
authors.lastname: Honda
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kawanobe
authors.forename: Ken
authors.initials: K
authors.lastname: Ishii
authors.forename: Toshiisa
authors.initials: T
authors.lastname: Konishi
authors.forename: Minori
authors.initials: M
authors.lastname: Mizumoto
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kanzawa
authors.forename: Morio
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Mamoru
authors.initials: M
authors.lastname: Aizawa
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects; D002118:Calcium / Q000737:chemistry; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D008163:Luminescent Measurements; D008297:Male; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D010006:Osteoblasts / Q000166:cytology; D019736:Prostheses and Implants; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology; D013010:Sonication; D013203:Staphylococcal Infections / Q000473:pathology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology
chemical_list: D000890:Anti-Infective Agents; D020099:Coated Materials, Biocompatible; D007477:Ions; D012834:Silver; D017886:Durapatite; D002118:Calcium
keywords: Ag; Ag-HAp; Antibacterial effect; Biofilm resistance; HAp; Implant-related infection; S. aureus; Silver-containing hydroxyapatite; Staphylococcus aureus; USSP; hydroxyapatite; silver; silver-containing hydroxyapatite; ultrasonic spray-pyrolysis