title: Extraction of lycopene from tomato paste by ursodeoxycholic acid using the selective inclusion complex method.
abstract: Lycopene, a precursor of β-carotene with well-known antioxidant activity and powerful health properties, can be found in many natural products such as tomato (Lycopersicon esculentum), watermelon, red pepper, and papaya. Many separation methods have been reported for extracting lycopene from its sources. The inclusion complex is an effective method for extraction and purification of organic chemicals. This procedure has 2 main components: host and guest molecules. In this study, lycopene (guest) was extracted from tomato paste by ursodeoxycholic acid, the inclusive agent (host). The molecular structure of the extracted lycopene was then confirmed by (1) HNMR and its purity was evaluated using high-performance liquid chromatography and UV-Vis spectrophotometry methods, in comparison with a standard product. The results indicated that the proposed separation method was very promising and could be used for the extraction and purification of lycopene from tomato paste.
authors.affiliation: Biotechnology Research Center, School of Pharmacy, Mashhad Univ. of Medical Sciences, Mashhad, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Seifi
authors.forename: Parisa
authors.initials: P
authors.lastname: Seifi
authors.forename: Farzin
authors.initials: F
authors.lastname: Hadizadeh
authors.forename: Seyed Ahmad
authors.initials: SA
authors.lastname: Mohajeri
mesh_terms: D002338:Carotenoids / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D005511:Food Handling; D000077276:Lycopene; D018551:Solanum lycopersicum / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013053:Spectrophotometry; D014580:Ursodeoxycholic Acid / Q000737:chemistry*; D019207:beta Carotene / Q000302:isolation & purification
chemical_list: D019207:beta Carotene; D002338:Carotenoids; D014580:Ursodeoxycholic Acid; D000077276:Lycopene
keywords: inclusive agent; lycopene; purification; ursodeoxycholic acid