title: Identification of a drought-induced rice gene, OsSAP, that suppresses Bax-induced cell death in yeast.
abstract: We identified rice genes that might be involved in drought stress tolerance by virtue of their anti-apoptotic activity. Potential anti-apoptosis related genes were identified by screening an Oryza sativa cDNA library derived from drought stressed tissues in a yeast functional assay. About 28 O. sativa cDNAs promoted yeast survival following engagement of Bax-induced apoptosis. An O. sativa cDNA encoding R12H780 was a highly conserved putative senescence-associated-protein (OsSAP). OsSAP was both highly and rapidly expressed in response to drought stress. Additionally, OsSAP was found to be localized to the mitochondria. Overall, OsSAP represents a new type of Bax suppressor related gene and endows multiple stress tolerance in yeast.
authors.affiliation: Division of Plant Biosciences, School of Applied BioSciences, College of Agriculture and Life Science, Kyungpook National University, Taegu, 702-701, Korea.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ubaidillah
authors.forename: Kyung-A
authors.initials: KA
authors.lastname: Kim
authors.forename: Yoon Ha
authors.initials: YH
authors.lastname: Kim
authors.forename: In-Jung
authors.initials: IJ
authors.lastname: Lee
authors.forename: Byung-Wook
authors.initials: BW
authors.lastname: Yun
authors.forename: Doh Hoon
authors.initials: DH
authors.lastname: Kim
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Loake
authors.forename: Kyung-Min
authors.initials: KM
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D018533:Biomass; D016922:Cellular Senescence / Q000235:genetics; D055864:Droughts; D015870:Gene Expression; D020869:Gene Expression Profiling; D017343:Genes, Plant; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D013312:Stress, Physiological / Q000235:genetics*; D014867:Water / Q000737:chemistry; D015003:Yeasts / Q000235:genetics* / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics* / Q000378:metabolism
chemical_list: D051028:bcl-2-Associated X Protein; D014867:Water