title: Expression of estrogen receptor α and β in esophageal squamous cell carcinoma.
abstract: Estrogen receptors (ERs) are frequently expressed in human tumor tissues. There have been several studies concerning ER expression in esophageal cancers, yet the results are inconsistent, and the prognostic value of the receptors remains unclear. In the present study, we investigated the expression of ER protein and its correlation with clinical features of esophageal squamous cell carcinoma (ESCC) patients. Immunohistochemical staining for the ERs was carried out on paraffin-embedded primary tumor tissue sections from 89 patients with ESCC. Quantitative analyses were performed to determine the prognostic value of the expression of ERs, and Pearson's correlation was used to examine the relationship between ERα and ERβ expression levels. Our results showed that ERα immunoreactivity was significantly lower in ESCC than that in the non-neoplastic epithelium (P=0.0445), whereas the ERβ status was much stronger in ESCC than that in the non-neoplastic epithelium (P=0.0243). A significant inverse correlation was observed between ERα expression and depth of tumor invasion (P=0.0426). Correlation analysis revealed a statistically significant inverse correlation between the expression of ERα and ERβ in ESCC (r=-0.2902, P=0.0058). Kaplan-Meier survival analysis showed that the patients with ERα expression (21/89) had a better outcome than patients without ERα expression (P=0.0280), whereas patients with high ERβ immunoreactivity (44/89) were significantly associated with worse survival (P=0.0366). In conclusion, ERα and ERβ levels were inversely correlated, and the downregulation of ERα and upregulation of ERβ may indicate unfavorable prognosis of ESCC.
authors.affiliation: Department of Histology and Embryology, Shantou University Medical College, Shantou, Guangdong, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Dong
authors.forename: Shi-Wen
authors.initials: SW
authors.lastname: Jiang
authors.forename: Yanru
authors.initials: Y
authors.lastname: Niu
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Shuyan
authors.initials: S
authors.lastname: Liu
authors.forename: Tianzhong
authors.initials: T
authors.lastname: Ma
authors.forename: Xiancai
authors.initials: X
authors.lastname: Chen
authors.forename: Liyan
authors.initials: L
authors.lastname: Xu
authors.forename: Zhongjing
authors.initials: Z
authors.lastname: Su
authors.forename: Haibin
authors.initials: H
authors.lastname: Chen
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D047628:Estrogen Receptor alpha / Q000096:biosynthesis* / Q000235:genetics; D047629:Estrogen Receptor beta / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D011379:Prognosis
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta