title: Identification of serum miRNAs as novel non-invasive biomarkers for detection of high risk for early gastric cancer.
abstract: BACKGROUND
  Many micro-RNAs (miRNAs) are differentially expressed in Helicobacter pylori-infected gastric mucosa and in gastric cancer tissue and previous reports have suggested the possibility of serum miRNAs as complementary tumour markers. The aim of the study was to investigate serum miRNAs and pepsinogen levels in individuals at high risk for gastric cancer both before and after H. pylori eradication.
  
  
  METHODS
  Patients with recent history of endoscopic resection for early gastric cancer and the sex- and age-matched controls were enrolled. Serum was collected from subjects before or after eradication and total RNA was extracted to analyse serum levels of 24 miRNAs. Serum pepsinogen (PG) I and II levels were measured using enzyme-linked immunosorbent assay kits.
  
  
  RESULTS
  Using miR-16 as an endogenous control, the relative levels of miR-106 and let-7d before and after H. pylori eradication and miR-21 after eradication were significantly higher in the high-risk group than in the controls. H. pylori eradication significantly decreased miR-106b levels and increased let-7d only in the control group. After eradication, the combination MiR-106b with miR-21 was superior to serum pepsinogen and the most valuable biomarker for the differentiating high-risk group from controls.
  
  
  CONCLUSIONS
  Serum miR-106b and miR-21 may provide a novel and stable marker of increased risk for early gastric cancer after H. pylori eradication.
authors.affiliation: Division of Gastroenterology, Department of Internal Medicine, Kawasaki Medical School, Kurashiki, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Shiotani
authors.forename: T
authors.initials: T
authors.lastname: Murao
authors.forename: Y
authors.initials: Y
authors.lastname: Kimura
authors.forename: H
authors.initials: H
authors.lastname: Matsumoto
authors.forename: T
authors.initials: T
authors.lastname: Kamada
authors.forename: H
authors.initials: H
authors.lastname: Kusunoki
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.forename: N
authors.initials: N
authors.lastname: Uedo
authors.forename: H
authors.initials: H
authors.lastname: Iishi
authors.forename: K
authors.initials: K
authors.lastname: Haruma
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000097:blood / Q000175:diagnosis / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000302:isolation & purification; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D020116:Pepsinogen A / Q000097:blood; D020115:Pepsinogen C / Q000097:blood; D013274:Stomach Neoplasms / Q000097:blood* / Q000235:genetics* / Q000382:microbiology / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C542541:MIRN106 microRNA, human; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D020115:Pepsinogen C; D020116:Pepsinogen A