title: Schmallenberg virus detection in bovine semen after experimental infection of bulls.
abstract: To study Schmallenberg virus (SBV) excretion in bovine semen after experimental infection, two bulls were inoculated subcutaneously with a SBV isolate (1 ml Vero cell culture 106 TCID50). After inoculation (at day 0), semen was collected daily from both animals for 21 days and samples were tested for SBV by qRT-PCR assay. At 24 days post-inoculation both animals were subjected to necropsy and the genital organs and lymph nodes draining these organs were also tested for SBV RNA (qRT-PCR). After SBV infection both animals in the study showed viraemia (qRT-PCR) with fever and diarrhoea. SBV RNA could be detected in semen from both animals. The highest SBV RNA concentrations in semen were found in the first week (days 4-7 post-inoculation) but concentrations were relatively low (Ct values 30-39). Viable SBV was only isolated from blood samples and not from semen or genital tissues.
authors.affiliation: Central Veterinary Institute (CVI) of Wageningen University and Research Centre,Lelystad,The Netherlands.
authors.forename: W H M
authors.initials: WH
authors.lastname: Van Der Poel
authors.affiliation: Faculty of Veterinary Medicine,Utrecht University,The Netherlands.
authors.forename: J M
authors.initials: JM
authors.lastname: Parlevliet
authors.affiliation: Central Veterinary Institute (CVI) of Wageningen University and Research Centre,Lelystad,The Netherlands.
authors.forename: E R A M
authors.initials: ER
authors.lastname: Verstraten
authors.affiliation: Central Veterinary Institute (CVI) of Wageningen University and Research Centre,Lelystad,The Netherlands.
authors.forename: E A
authors.initials: EA
authors.lastname: Kooi
authors.affiliation: Central Veterinary Institute (CVI) of Wageningen University and Research Centre,Lelystad,The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Hakze-Van Der Honing
authors.affiliation: Central Veterinary Institute (CVI) of Wageningen University and Research Centre,Lelystad,The Netherlands.
authors.forename: N
authors.initials: N
authors.lastname: Stockhofe
mesh_terms: D000818:Animals; D002044:Bunyaviridae Infections / Q000662:veterinary* / Q000821:virology; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D002522:Chlorocebus aethiops; D021821:Communicable Diseases, Emerging / Q000821:virology; D005837:Genitalia, Male / Q000821:virology; D008198:Lymph Nodes / Q000821:virology; D008297:Male; D016851:Orthobunyavirus / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis; D012661:Semen / Q000821:virology*; D014709:Vero Cells
chemical_list: D012367:RNA, Viral