title: Dual-wavelength laser speckle imaging to simultaneously access blood flow, blood volume, and oxygenation using a color CCD camera.
abstract: We developed a dual-wavelength laser speckle imaging system using a single industrial-grade color CCD camera with Bayer filters to simultaneously image changes in blood flow, blood volume, and oxygenation. One frame of a color image recorded with dual-wavelength laser illumination provides not only the intensity fluctuation of the speckle pattern, but also the dual-wavelength optical reflectance signal. The method was validated using a tissue phantom and cuff ischemia experiments in the human arm. This system achieves complete time synchronization, unlike conventional time-sharing systems. Compared with a multicamera system, it also avoids the problem of image registration and can be less expensive.
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.forename: Yaru
authors.initials: Y
authors.lastname: Wang
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Danqi
authors.initials: D
authors.lastname: Feng
authors.forename: Jinling
authors.initials: J
authors.lastname: Lu
authors.forename: Qingming
authors.initials: Q
authors.lastname: Luo
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Li
mesh_terms: D001775:Blood Circulation; D001810:Blood Volume; D003116:Color; D005385:Fingers / Q000098:blood supply; D006801:Humans; D061848:Optical Imaging / Q000295:instrumentation*; D010100:Oxygen / Q000378:metabolism*; D013997:Time Factors
chemical_list: D010100:Oxygen