title: Influence of DNMT genotype on global and site specific DNA methylation patterns in neonates and pregnant women.
abstract: This study examines the relationship between common genetic variation within DNA methyltransferase genes and inter-individual variation in DNA methylation. Eleven polymorphisms spanning DNMT1 and DNMT3B were genotyped. Global and gene specific (IGF2, IGFBP3, ZNT5) DNA methylation was quantified by LUMA and bisulfite Pyrosequencing assays, respectively, in neonatal cord blood and in maternal peripheral blood. Associations between maternal genotype and maternal methylation (n (≈) 333), neonatal genotype and neonatal methylation (n (≈) 454), and maternal genotype and neonatal methylation (n (≈) 137) were assessed. The findings of this study provide some support to the hypothesis that genetic variation in DNA methylating enzymes influence DNA methylation at global and gene-specific levels; however observations were not robust to correction for multiple testing. More comprehensive analysis of the influence of genetic variation on global and site specific DNA methylation is warranted.
authors.affiliation: Human Nutrition Research Centre, Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne, Tyne and Wear, United Kingdom.
authors.forename: Catherine
authors.initials: C
authors.lastname: Potter
authors.forename: Jill
authors.initials: J
authors.lastname: McKay
authors.forename: Alexandra
authors.initials: A
authors.lastname: Groom
authors.forename: Dianne
authors.initials: D
authors.lastname: Ford
authors.forename: Lisa
authors.initials: L
authors.lastname: Coneyworth
authors.forename: John C
authors.initials: JC
authors.lastname: Mathers
authors.forename: Caroline L
authors.initials: CL
authors.lastname: Relton
mesh_terms: D000328:Adult; D027682:Cation Transport Proteins / Q000235:genetics; D018899:CpG Islands; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics*; D019175:DNA Methylation; D005260:Female; D056426:Genetic Loci; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000235:genetics; D007335:Insulin-Like Growth Factor II / Q000235:genetics; D015810:Linkage Disequilibrium; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D000097584:DNA Methyltransferase 3B
chemical_list: D027682:Cation Transport Proteins; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; D018972:Insulin-Like Growth Factor Binding Protein 3; D007335:Insulin-Like Growth Factor II; D000097584:DNA Methyltransferase 3B; C035170:IGF2 protein, human; C515497:IGFBP3 protein, human; C457225:SLC30A5 protein, human; C000619380:DNMT1 protein, human