title: The increasing application of multiplex nucleic acid detection tests to the diagnosis of syndromic infections.
abstract: On 14 January 2013, the US Food and Drug Administration (FDA) announced permission for a multiplex nucleic acid test, the xTAG® Gastrointestinal Pathogen Panel (GPP) (Luminex Corporation, USA), which simultaneously detects 11 common viral, bacterial and parasitic causes of infectious gastroenteritis, to be marketed in the USA. This announcement reflects the current move towards the development and commercialization of detection technologies based on nucleic acid amplification techniques for diagnosis of syndromic infections. We discuss the limitations and advantages of nucleic acid amplification techniques and the recent advances in Conformité Européene - in-vitro diagnostic (CE-IVD)-approved multiplex real-time PCR kits for the simultaneous detection of multiple targets within the clinical diagnostics market.
authors.affiliation: Norwich Medical School, University of East Anglia, NRP Innovation Centre, Norwich Research Park, Norwich, Norfolk, UK.
authors.forename: J
authors.initials: J
authors.lastname: Gray
authors.forename: L J
authors.initials: LJ
authors.lastname: Coupland
mesh_terms: D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D005759:Gastroenteritis / Q000175:diagnosis* / Q000382:microbiology / Q000821:virology; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods* / Q000639:trends; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D014777:Virus Diseases / Q000175:diagnosis* / Q000821:virology
chemical_list: D011933:Reagent Kits, Diagnostic