title: Arabidopsis receptor-like protein30 and receptor-like kinase suppressor of BIR1-1/EVERSHED mediate innate immunity to necrotrophic fungi.
abstract: Effective plant defense strategies rely in part on the perception of non-self determinants, so-called microbe-associated molecular patterns (MAMPs), by transmembrane pattern recognition receptors leading to MAMP-triggered immunity. Plant resistance against necrotrophic pathogens with a broad host range is complex and yet not well understood. Particularly, it is unclear if resistance to necrotrophs involves pattern recognition receptors. Here, we partially purified a novel proteinaceous elicitor called sclerotinia culture filtrate elicitor1 (SCFE1) from the necrotrophic fungal pathogen Sclerotinia sclerotiorum that induces typical MAMP-triggered immune responses in Arabidopsis thaliana. Analysis of natural genetic variation revealed five Arabidopsis accessions (Mt-0, Lov-1, Lov-5, Br-0, and Sq-1) that are fully insensitive to the SCFE1-containing fraction. We used a forward genetics approach and mapped the locus determining SCFE1 sensitivity to receptor-like protein30 (RLP30). We also show that SCFE1-triggered immune responses engage a signaling pathway dependent on the regulatory receptor-like kinases brassinosteroid insensitive1-associated receptor kinase1 (BAK1) and Suppressor of BIR1-1/evershed (SOBIR1/EVR). Mutants of RLP30, BAK1, and SOBIR1 are more susceptible to S. sclerotiorum and the related fungus Botrytis cinerea. The presence of an elicitor in S. sclerotiorum evoking MAMP-triggered immune responses and sensed by RLP30/SOBIR1/BAK1 demonstrates the relevance of MAMP-triggered immunity in resistance to necrotrophic fungi.
authors.affiliation: Department of Biochemistry, Center for Plant Molecular Biology, Eberhard Karls University, D-72076 Tuebingen, Germany.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Zhang
authors.forename: Malou
authors.initials: M
authors.lastname: Fraiture
authors.forename: Dagmar
authors.initials: D
authors.lastname: Kolb
authors.forename: Birgit
authors.initials: B
authors.lastname: Löffelhardt
authors.forename: Yoshitake
authors.initials: Y
authors.lastname: Desaki
authors.forename: Freddy F G
authors.initials: FF
authors.lastname: Boutrot
authors.forename: Mahmut
authors.initials: M
authors.lastname: Tör
authors.forename: Cyril
authors.initials: C
authors.lastname: Zipfel
authors.forename: Andrea A
authors.initials: AA
authors.lastname: Gust
authors.forename: Frédéric
authors.initials: F
authors.lastname: Brunner
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000276:immunology*; D001203:Ascomycota / Q000472:pathogenicity*; D060467:Disease Resistance / Q000235:genetics; D005656:Fungal Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D057865:Plant Immunity; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000276:immunology*
chemical_list: D029681:Arabidopsis Proteins; D005656:Fungal Proteins; C463660:BAK1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases