title: Extraction and RP-HPLC determination of taxol in rat plasma, cell culture and quality control samples.
abstract: A rapid, sensitive, selective and validated reverse phase high-performance liquid chromatography (RP-HPLC) method for the estimation of paclitaxel in micro-sample of rat plasma and in culture of cancer cells was performed in this study. The mobile phase consisted of an optimized mixture of methanol:water: trifluroacetic acid (80: 20: 0.1, v/v/v). Column elution at a flow rate of 1 mL/minute with UV detection at 225 nm at room temperature was used. The RP-HPLC method was successfully applied for the determination of paclitaxel in plasma samples and in culture of cancer cells with nano-quantity of estimation. The validation studies were performed in accordance with the International Conference on Harmonization (ICH) guidelines. The intra- and inter-day precision showed that the coefficients of variation ranged from 1.07% to 4.27% at different levels of concentrations. To the best of our knowledge, this study also reported for the first time the optimization of different solvents for effective extraction of paclitaxel wherein tert.-butyl methyl ether (TBME): diethyl ether (DEE) in 50: 50 v/v composition was found most efficient with extraction efficiency ranging between 77.99% and 91.74% and between 76.14 and 93.66% in the plasma and cell culture, respectively. This proposed method was successfully applied to study the pharmacokinetics of paclitaxel and the influence of verapamil and all-trans retinoic acid (atRA) on paclitaxel pharmacokinetics in rat models. This proposed method might emerge as a valuable aid in the laboratory monitoring of paclitaxel in a variety of in vitro as well as in vivo scenarios.
authors.affiliation: School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston PR1 2HE United Kingdom; ; College of Pharmacy, University of Hawai'i at Hilo, 96720, Hawai'i, USA; ; Pharmaceutics Research Laboratory, Dr. Hari Singh Gour University, Sagar (M.P), 470003 India;
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Tekade
authors.forename: Antony
authors.initials: A
authors.lastname: D'Emanuele
authors.forename: Abdelbary
authors.initials: A
authors.lastname: Elhissi
authors.forename: Ashish
authors.initials: A
authors.lastname: Agrawal
authors.forename: Anurekha
authors.initials: A
authors.lastname: Jain
authors.forename: Basel Tawfiq
authors.initials: BT
authors.lastname: Arafat
authors.forename: Narendra Kumar
authors.initials: NK
authors.lastname: Jain
keywords: extraction optimization; high-performance liquid chromatography (HPLC); micro-sample rat plasma; paclitaxel; plasma profile