title: A fast fluorescence imaging flow cytometer for phytoplankton analysis.
abstract: We report a fast fluorescence imaging flow cytometer for phytoplankton analysis that can achieve a volume flow rate up to 1ml/min. The instrument shows a high immunity to motion blur in image captured with a lateral resolution of 0.75 ± 0.06 μm for a wide size range ~1 μm to ~200 μm. This is made possible by suppressing the out-of-focus light using thin light sheet illumination and image deconvolution, and by precluding the motion-blur with a unique flow configuration. Preliminary results from untreated coastal water samples show the technique has high potential as a practical field instrument for monitoring phytoplankton abundance and species composition.
authors.forename: Jianglai
authors.initials: J
authors.lastname: Wu
authors.forename: Robert K Y
authors.initials: RK
authors.lastname: Chan
mesh_terms: D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D005453:Fluorescence; D007091:Image Processing, Computer-Assisted; D010839:Phytoplankton / Q000166:cytology*; D012623:Seawater / Q000382:microbiology