title: All-transretinoic acid regulates Th1/Th2 balance in CD4+ T cells when GATA-3 is deficient.
abstract: The essential effect of vitamin A on immune function occurs through various mechanisms including direct effect on Th1-Th2 balance modulation. However, it is unclear whether or not vitamin A can regulate Th1-Th2 balance under a strong Th1-polarizing condition. Therefore, the purpose of our study was to examine the effect of vitamin A metabolite all-trans retinoic acid (ATRA) on Th1-Th2 differentiation in CD4+ T cells under GATA-3 deficiency, which can induce Th1-polarizing condition. In the present study, GATA-3 deficiency T cells were induced by siRNA and checked by real-time quantitative PCR and western blot. GATA-3 deficiency CD4+ T cells and normal CD4+ T were treated for 48 h with or without ATRA. The expression of Th1 and Th2 cytokines were detected by qPCR and ELISA. The results would contribute to clarify the knowledge of the role of vitamin A in regulating Th1-Th2 balance under some special conditions, and help to explain the mechanism of immune regulatory function of vitamin A.
authors.affiliation: Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, Sichuan, China.
authors.forename: Yan Feng
authors.initials: YF
authors.lastname: Zhu
authors.forename: Jia Zhe
authors.initials: JZ
authors.lastname: Hu
authors.forename: Pin Nan
authors.initials: PN
authors.lastname: Zhao
authors.forename: Lin Xi
authors.initials: LX
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D050990:GATA3 Transcription Factor / Q000172:deficiency*; D006801:Humans; D058408:Th1-Th2 Balance / Q000187:drug effects*; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; D014212:Tretinoin