title: Immobilization of an esterase inhibitor on a porous hollow-fiber membrane by radiation-induced graft polymerization for developing a diagnostic tool for feline kidney diseases.
abstract: Removal of the major urinary protein, cauxin, a carboxylesterase, from cat urine is essential for distinguishing between physiological and abnormal proteinuria by a urine dipstick. We have previously developed a material for removing cauxin by using lens culinaris agglutinin (LCA) lectin which targets the N-linked oligosaccharides present in cauxin. To improve the affinity and specificity toward cauxin, we immobilized 1,1,1-trifluoro-3-(2-sulfanylethylsulfanyl) propane-2-one, an inhibitor of esterases, to a polymer chain grafted on to a porous hollow-fiber membrane by applying radiation-induced graft polymerization. Normal male urine was forced to permeate through the pores rimmed by the ligand-immobilized polymer chain. Cauxin could not be detected in the effluent from the membrane. The residence time of the urine across a membrane thickness of 1 mm was set at 7 s. The respective dynamic and equilibrium binding capacities of the membrane for cauxin were 2 and 3 mg/g. The developed cauxin-affinity membrane material was more effective for diagnosing cat kidney diseases than the LCA lectin tip.
authors.affiliation: Department of Applied Chemistry and Biotechnology, Chiba University.
authors.forename: Shinya
authors.initials: S
authors.lastname: Matsuno
authors.forename: Daisuke
authors.initials: D
authors.lastname: Umeno
authors.forename: Masao
authors.initials: M
authors.lastname: Miyazaki
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Suzuta
authors.forename: Kyoichi
authors.initials: K
authors.lastname: Saito
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Yamashita
mesh_terms: D000096:Acetone / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D043182:Carboxylesterase / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine; D002371:Cat Diseases / Q000175:diagnosis* / Q000652:urine; D002415:Cats; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D004950:Esterases / Q000037:antagonists & inhibitors*; D007674:Kidney Diseases / Q000175:diagnosis* / Q000652:urine; D008024:Ligands; D008297:Male; D008567:Membranes, Artificial; D058105:Polymerization; D016062:Porosity; D013440:Sulfides / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D008024:Ligands; D008567:Membranes, Artificial; D013440:Sulfides; D000096:Acetone; D004950:Esterases; D043182:Carboxylesterase; C515680:cauxin protein, Felis catus