title: Reflection-mode optical-resolution photoacoustic microscopy based on a reflective objective.
abstract: We developed a new reflection-mode optical-resolution photoacoustic microscopy (OR-PAM) based on the cooperation of a reflective objective and an ultrasonic transducer. The reflective objective is used to produce nearly diffraction-limited optical focusing, and the excited ultrasound waves are then directly detected by an ultrasonic transducer that was placed in the central cone of the objective. This new design avoids the coupling between optical focusing and ultrasound transmission in the reflection mode. Moreover, the proposed system is able to provide lateral resolution of 1.2 μm at 580 nm, penetration depth of 0.9 mm in biological tissues, and a work distance of 6.0 mm. We present in vivo imaging of the microvasculature in mouse ears and in vitro imaging of red blood cells (RBCs), which demonstrate the capability of the system to study microcirculation.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Yang
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Liu
authors.forename: Bowen
authors.initials: B
authors.lastname: Jiang
authors.forename: Qingming
authors.initials: Q
authors.lastname: Luo
mesh_terms: D000818:Animals; D004423:Ear / Q000033:anatomy & histology / Q000098:blood supply; D004912:Erythrocytes / Q000166:cytology; D021621:Imaging, Three-Dimensional; D051379:Mice; D008853:Microscopy / Q000379:methods*; D055806:Microvessels / Q000033:anatomy & histology; D055620:Optical Phenomena; D061088:Photoacoustic Techniques / Q000379:methods*