title: [Tryptase of mast cells in immune organs of developing chicken embryos].
abstract: OBJECTIVE
  To observe the form, distribution and emerging time of the mast cells (MCs) and tryptase positive cells (TPCs) in the immune organs of different days old chicken embryos.
  
  
  METHODS
  The thymus, bursa of Fabricius and spleen of chicken embryos at different development time were taken and fixed in Carnoy's solution. The alcian blue/safranine O (AB/SO) and streptavidin-biotin complex (SABC) immunohistochemistry were used to observe the MCs and TPCs.
  
  
  RESULTS
  MCs were first present in the thymus, bursa and spleen of day 13-14 embryos. The number of MCs increased along with the development days. On day 16, TPCs were first found in the immune organs. The form and distribution of TPCs were similar to MCs, but the emerging time of TPCs was later than that of MCs.
  
  
  CONCLUSIONS
  Tryptase appears in the MCs in the immune organs of day 16 or later chicken embryos, and it is mainly located in the MCs of the connective tissues.
authors.affiliation: College of Animal Sciences, Guizhou University, Guiyang 550025, China.
authors.forename: Denghui
authors.initials: D
authors.lastname: Gao
authors.forename: Junfeng
authors.initials: J
authors.lastname: Guo
authors.forename: Deyuan
authors.initials: D
authors.lastname: Ou
authors.forename: Hongyan
authors.initials: H
authors.lastname: Yao
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
mesh_terms: D000818:Animals; D002060:Bursa of Fabricius / Q000201:enzymology; D002642:Chick Embryo; D047108:Embryonic Development; D008407:Mast Cells / Q000201:enzymology*; D013154:Spleen / Q000201:enzymology; D013950:Thymus Gland / Q000201:enzymology; D053802:Tryptases / Q000032:analysis*
chemical_list: D053802:Tryptases