title: On-chip cellomics: Single-cell-based constructive cell-network assay for quasi-in vivo screening of cardiotoxicity.
abstract: We have developed methods and systems of analyzing epigenetic information in cells, as well as that of genetic information, to expand our understanding of how living systems are determined. A system of analyzing epigenetic information was developed starting from the twin complementary viewpoints of cell regulation as an 'algebraic' system (emphasis on temporal aspects) and as a 'geometric' system (emphasis on spatial aspects). As an example of the 'geometric' system, we have developed an quasi-in vivo hiPS cardiomyocyte network assay and confirmed that it can predict the risk of lethal arrythmia correctly in 22 compounds. The knowlege acquired from this study may lead to the use of cells that fully control practical applications like cell-based drug screening and the regeneration of organs.
authors.forename: Kenji
authors.initials: K
authors.lastname: Yasuda
mesh_terms: D000818:Animals; D001145:Arrhythmias, Cardiac / Q000139:chemically induced*; D016466:CHO Cells; D066126:Cardiotoxicity; D006224:Cricetinae; D003412:Cricetulus; D004353:Drug Evaluation, Preclinical / Q000379:methods; D044127:Epigenesis, Genetic; D057809:HEK293 Cells; D006801:Humans; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D059010:Single-Cell Analysis