title: The ha72 core gene of baculovirus is essential for budded virus production and occlusion-derived virus embedding, and amino acid K22 plays an important role in its function.
abstract: ha72 of Helicoverpa armigera nucleopolyhedrovirus (a homologue of ac78) was identified as a conserved late baculovirus gene and characterized. HA72 localizes in the intranuclear ring zone. By generating mutants, we showed that HA72 is essential for budded virus (BD) production and occlusion-derived virus (ODV) embedding. HA72 also interacted with P33, a baculoviral sulfhydryl oxidase. A point mutation of amino acid 22 from lysine to glutamic acid curtailed BV production and precluded ODV occlusion as well as interaction with P33.
authors.affiliation: State Key Laboratory of Virology and Joint Laboratory of Invertebrate Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, People's Republic of China.
authors.forename: Huachao
authors.initials: H
authors.lastname: Huang
authors.forename: Manli
authors.initials: M
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Deng
authors.forename: Dianhai
authors.initials: D
authors.lastname: Hou
authors.forename: Basil M
authors.initials: BM
authors.lastname: Arif
authors.forename: Hualin
authors.initials: H
authors.lastname: Wang
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000502:physiology*; D016367:Baculoviridae / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D017931:DNA Primers; D005814:Genes, Viral; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D017931:DNA Primers; D014764:Viral Proteins