title: Expression, crystallization and preliminary X-ray crystallographic analysis of cellobiose 2-epimerase from Dictyoglomus turgidum DSM 6724.
abstract: Cellobiose 2-epimerase epimerizes and isomerizes β-1,4- and α-1,4-gluco-oligosaccharides. N-Acyl-D-glucosamine 2-epimerase (DT_epimerase) from Dictyoglomus turgidum has an unusually high catalytic activity towards its substrate cellobiose. DT_epimerase was expressed, purified and crystallized. Crystals were obtained of both His-tagged DT_epimerase and untagged DT_epimerase. The crystals of His-tagged DT_epimerase diffracted to 2.6 Å resolution and belonged to the monoclinic space group P2₁, with unit-cell parameters a=63.9, b=85.1, c=79.8 Å, β=110.8°. With a Matthews coefficient VM of 2.18 Å3 Da(-1), two protomers were expected to be present in the asymmetric unit with a solvent content of 43.74%. The crystals of untagged DT_epimerase diffracted to 1.85 Å resolution and belonged to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a=55.9, b=80.0, c=93.7 Å. One protomer in the asymmetric unit was expected, with a corresponding VM of 2.26 Å3 Da(-1) and a solvent content of 45.6%.
authors.affiliation: Department of Advanced Technology Fusion, Konkuk University, 1 Hwayang dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Tan-Viet
authors.initials: TV
authors.lastname: Pham
authors.forename: Seung-Hye
authors.initials: SH
authors.lastname: Hong
authors.forename: Myoung-ki
authors.initials: MK
authors.lastname: Hong
authors.forename: Ho-Phuong-Thuy
authors.initials: HP
authors.lastname: Ngo
authors.forename: Deok-Kun
authors.initials: DK
authors.lastname: Oh
authors.forename: Lin-Woo
authors.initials: LW
authors.lastname: Kang
mesh_terms: D001419:Bacteria / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry*; D002238:Carbohydrate Epimerases / Q000737:chemistry*; D002352:Carrier Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D006639:Histidine; D009842:Oligopeptides
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; C471213:His-His-His-His-His-His; D009842:Oligopeptides; D006639:Histidine; D002238:Carbohydrate Epimerases; C024519:N-acyl-D-glucosamine 2-epimerase
keywords: Dictyoglomus turgidum; cellobiose 2-epimerase