title: Simultaneous decolorization of reactive Orange M2R dye and reduction of chromate by Lysinibacillus sp. KMK-A.
abstract: Azo dyes constitute the largest and diverse group of dyes, widely used in number of industries that are contributing toward organic and inorganic load of effluent treatment. In the present study, Lysinibacillus sp. KMK-A was able to effectively decolorize Orange M2R dye up to 2000 mg l(-1) (Vmax of 19.6 mg l(-1) h(-1) and Km of 439 mg l(-1)) and reduce Cr(VI) up to 250 mg l(-1) (Vmax of 3.6 mg l(-1) h(-1) and Km 28.3 mg l(-1)). It also has an ability of simultaneous decolorization of Orange M2R dye (200-1000 mg l(-1)) with reduction of Cr(VI) (50-200 mg l(-1)). Significant reduction in total organic carbon content, chemical and biological oxygen demand along with spectroscopic and chromatographic analysis confirmed the biotransformation of Orange M2R. Involvement of enzymes namely azoreductase and chromate reductase was observed during biotransformation. The phyto and geno toxicity studies demonstrated that metabolites of dye degradation were non-toxic. Higher tolerance with simultaneous decolorization and detoxification of azo dyes in presence of Cr(VI) makes Lysinibacillus sp. KMK-A, a potential candidate for eco-friendly remediation of metal contaminated dye effluents.
authors.affiliation: Biochemistry Division, Department of Chemistry, University of Pune, Pune 411007, India.
authors.forename: Ashvini U
authors.initials: AU
authors.lastname: Chaudhari
authors.forename: Savita R
authors.initials: SR
authors.lastname: Tapase
authors.forename: Vijaykumar L
authors.initials: VL
authors.lastname: Markad
authors.forename: Kisan M
authors.initials: KM
authors.lastname: Kodam
mesh_terms: D001391:Azo Compounds / Q000378:metabolism* / Q000633:toxicity; D001406:Bacillaceae / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001483:Base Sequence; D002478:Cells, Cultured; D002840:Chromates / Q000378:metabolism; D003116:Color; D004396:Coloring Agents / Q000378:metabolism* / Q000633:toxicity; D020552:Comet Assay; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism* / Q000633:toxicity
chemical_list: D001391:Azo Compounds; D002840:Chromates; D004396:Coloring Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D014874:Water Pollutants, Chemical
keywords: Azo dyes; Azoreductase; Biotransformation; Chromate reduction; Toxicity