title: Mineral trioxide aggregate enhances the odonto/osteogenic capacity of stem cells from inflammatory dental pulps via NF-κB pathway.
abstract: OBJECTIVE
  This study was designed to investigate the effects of mineral trioxide aggregate (MTA) on the osteo/odontogenic differentiation of inflammatory dental pulp stem cells (iDPSCs).
  
  
  METHODS
  inflammatory DPSCs were isolated from the inflammatory pulps of rat incisors and cocultured with MTA-conditioned medium. MTT assay and flow cytometry were performed to evaluate the proliferation of iDPSCs. Alkaline phosphatase (ALP) activity, alizarin red staining, real-time RT-PCR, and Western blot assay were used to investigate the differentiation capacity as well as the involvement of NF-κB pathway in iDPSCs.
  
  
  RESULTS
  Mineral trioxide aggregate-treated iDPSCs demonstrated the higher ALP activity and formed more mineralized nodules than the untreated group. The odonto/osteoblastic markers (Alp, Runx2/RUNX2, Osx/OSX, Ocn/OCN, and Dspp/DSP, respectively) in MTA-treated iDPSCs were significantly upregulated as compared with untreated iDPSCs. Mechanistically, cytoplastic phos-P65 and nuclear P65 in MTA-treated iDPSCs were significantly increased in a time-dependent manner. Moreover, the inhibition of NF-κB pathway suppressed the MTA-induced odonto/osteoblastic differentiation of iDPSCs, as indicated by decreased ALP levels, weakened mineralization capacity and downregulated levels of odonto/osteoblastic genes (Osx, Ocn, and Dspp).
  
  
  CONCLUSIONS
  Mineral trioxide aggregate enhances the odonto/osteogenic capacity of DPSCs from inflammatory sites via activating the NF-κB pathway.
authors.affiliation: Institute of Stomatology, Nanjing Medical University, Nanjing, China; Endodontic Department, Affiliated Stomatological Hospital of Soochow University, Suzhou Stomatological Hospital, Suzhou, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Yan
authors.forename: Z
authors.initials: Z
authors.lastname: Fan
authors.forename: L
authors.initials: L
authors.lastname: Ma
authors.forename: Y
authors.initials: Y
authors.lastname: Yu
authors.forename: J
authors.initials: J
authors.lastname: Yu
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology*; D000818:Animals; D017610:Calcium Compounds / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D003782:Dental Pulp / Q000166:cytology*; D004338:Drug Combinations; D016328:NF-kappa B / Q000187:drug effects* / Q000502:physiology*; D009805:Odontogenesis / Q000187:drug effects* / Q000502:physiology*; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology*; D010087:Oxides / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017640:Silicates / Q000494:pharmacology*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects*
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D016328:NF-kappa B; D010087:Oxides; D017640:Silicates; C086631:mineral trioxide aggregate
keywords: dental pulp stem cells; differentiation; inflammation; mineral trioxide aggregate; nuclear factor kappa B