title: Analysis of phytochemical profile of Terminalia arjuna bark extract with antioxidative and antimicrobial properties.
abstract: OBJECTIVE
  To investigate phytochemical screening, antimicrobial activity and qualitative thin layer chromatographic separation of flavonoid components, antioxidant activity and total flavonoid compound of Terminalia arjuna.
  
  
  METHODS
  For phytochemical screening, some common and available standard tests were done. Antimicrobial bioassay was done through agar well diffusion method. Detection of antioxidant activity and flavonoid compounds were done through thin layer chromatography. Total antioxidant activity was measured by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) in colorimetric method. Aluminum chloride colorimetric method was used for total flavonoid determination.
  
  
  RESULTS
  Phytochemical screening showed the active compounds presence in high concentration, such as phytosterol, lactones, flavonoids, phenolic compounds and tannins and glycosides. The antimicrobial activity of extract showed that greater inhibition zone against Gram negative bacteria than Gram positive bacteria. This methanolic extract showed a promising antioxidant activity, as absorption of DPPH redicles decreased in DPPH free radical scavenging assay. Flavonoids components having antioxidant property present in the methanol extract at a level of 199.00 mg quercetin equivalent/g of dried methanol extract in colorimetric method.
  
  
  CONCLUSIONS
  The Terminalia arjuna bark extract revealed the presence of bio-active constituents which are known to exhibit medicinal as well as physiological activities.
authors.affiliation: Department of Nutrition, Microbiology and Human Physiology, Raja N L Khan Women's College, Midnapore, Pin-721102, West Bengal, India.
authors.forename: Shreya
authors.initials: S
authors.lastname: Mandal
authors.forename: Arpita
authors.initials: A
authors.lastname: Patra
authors.forename: Animesh
authors.initials: A
authors.lastname: Samanta
authors.forename: Suchismita
authors.initials: S
authors.lastname: Roy
authors.forename: Arpita
authors.initials: A
authors.lastname: Mandal
authors.forename: Tapasi Das
authors.initials: TD
authors.lastname: Mahapatra
authors.forename: Shrabani
authors.initials: S
authors.lastname: Pradhan
authors.forename: Koushik
authors.initials: K
authors.lastname: Das
authors.forename: Dilip Kumar
authors.initials: DK
authors.lastname: Nandi
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D006094:Gram-Positive Bacteria; D008826:Microbial Sensitivity Tests; D064209:Phytochemicals / Q000737:chemistry* / Q000302:isolation & purification; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D029766:Terminalia / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D064209:Phytochemicals; D010936:Plant Extracts
keywords: Antimicrobial activity; Antioxidant; DPPH; Phytocompound; TLC; Terminalia arjuna; Total flavonoid