title: Blood volatile compounds as biomarkers for colorectal cancer.
abstract: Many recent studies have focused on the connection between the composition of specific volatile organic compounds (VOCs) in exhaled breath and various forms of cancer. However, the composition of exhaled breath is affected by many factors, such as lung disease, smoking, and diet. VOCs are released into the bloodstream before they are exhaled; therefore, the analysis of VOCs in blood will provide more accurate results than the analysis of VOCs in exhaled breath. Blood were collected from 16 colorectal cancer patients and 20 healthy controls, then solid phase microextraction-chromatography-mass spectrometry (SPME-GC-MS) was used to analysis the exhaled volatile organic compounds (VOCs). The statistical methods principal component analysis (PCA) and partial least-squares discriminant analysis (PLSDA) were performed to deal with the final dates. Three metabolic biomarkers were found at significantly lower levels in the group of CRC patients than in the normal control group (P<0.01): phenyl methylcarbamate, ethylhexanol, and 6-t-butyl-2,2,9,9-tetramethyl-3,5-decadien-7-yne. In addition, significantly higher levels of 1,1,4,4-tetramethyl-2,5-dimethylene-cyclohexane were found in the group of CRC patients than in the normal control group (P<0.05). Compared with healthy individuals, patients with colorectal adenocarcinoma exhibited a distinct blood metabolic profile with respect to VOCs. The analysis of blood VOCs appears to have potential clinical applications for CRC screening.
authors.affiliation: Department of Anesthesiology; First Affiliated Hospital of Harbin Medical University; Harbin, PR China.
authors.forename: Changsong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology; First Affiliated Hospital of Harbin Medical University; Harbin, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Anesthesiology; First Affiliated Hospital of Harbin Medical University; Harbin, PR China.
authors.forename: Ailing
authors.initials: A
authors.lastname: Lian
authors.affiliation: Department of Anesthesiology; First Affiliated Hospital of Harbin Medical University; Harbin, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Anesthesiology; First Affiliated Hospital of Harbin Medical University; Harbin, PR China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology; First Affiliated Hospital of Harbin Medical University; Harbin, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Anesthesiology; First Affiliated Hospital of Harbin Medical University; Harbin, PR China.
authors.forename: Chunjie
authors.initials: C
authors.lastname: Chi
authors.affiliation: Department of Anesthesiology; First Affiliated Hospital of Harbin Medical University; Harbin, PR China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology; First Affiliated Hospital of Harbin Medical University; Harbin, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Anesthesiology; First Affiliated Hospital of Harbin Medical University; Harbin, PR China.
authors.forename: Suqi
authors.initials: S
authors.lastname: Luo
authors.affiliation: Department of Anesthesiology; First Affiliated Hospital of Harbin Medical University; Harbin, PR China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Department of Anesthesiology; First Affiliated Hospital of Harbin Medical University; Harbin, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Biostatistics; School of Public Health; Harbin Medical University; Harbin, PR China.
authors.forename: Chaofu
authors.initials: C
authors.lastname: Ke
authors.affiliation: CAS Key Laboratory of Separation Science for Analytical Chemistry; Dalian Institute of Chemical Physics; Chinese Academy of Sciences; Dalian, PR China.
authors.forename: Guozhu
authors.initials: G
authors.lastname: Ye
authors.affiliation: CAS Key Laboratory of Separation Science for Analytical Chemistry; Dalian Institute of Chemical Physics; Chinese Academy of Sciences; Dalian, PR China.
authors.forename: Guowang
authors.initials: G
authors.lastname: Xu
authors.affiliation: The Heilongjiang Key Laboratory of Immunity and Infection; Pathogenic Biology; Department of Microbiology; Harbin Medical University; Harbin, PR China; Key Laboratory of Bio-Pharmaceutical; Harbin Medical University; Ministry of Education; Harbin, PR China.
authors.forename: Fengmin
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology; First Affiliated Hospital of Harbin Medical University; Harbin, PR China.
authors.forename: Enyou
authors.initials: E
authors.lastname: Li
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000175:diagnosis*; D000368:Aged; D000466:Alkadienes / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D001944:Breath Tests; D002219:Carbamates / Q000097:blood*; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000097:blood* / Q000175:diagnosis; D005260:Female; D000441:Hexanols / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: C000592915:6-t-butyl-2,2,9,9-tetramethyl-3,5-decadien-7-yne; D000466:Alkadienes; D014408:Biomarkers, Tumor; D002219:Carbamates; D000441:Hexanols; C034017:2-ethylhexanol
keywords: biomarkers; cancer diagnosis; colorectal cancer; solid-phase microextraction; volatile organic compounds