title: Effects of morphine and fentanyl on 5-fluorouracil sensitivity in human colon cancer HCT116 cells.
abstract: Opioids are widely used for perioperative pain management in cancer surgery patients. It has been reported that opioids may alter cancer recurrence or progression; however, there are no published reports regarding the effects of opioids on chemotherapy after cancer surgery. Here we investigated the effects of opioids (morphine or fentanyl) on cell proliferation and 5-fluorouracil sensitivity in the human colon cancer cell line, HCT116. First, we exposed cancer cells to the opioid at various concentrations for 6 or 24 h and evaluated cell proliferation using a MTT assay. Next, to simulate the potential postoperative situation in which anticancer drugs are administered after cancer surgery, cancer cells were incubated with the opioid for 6 or 24 h, followed by treatment with 5-fluorouracil for 48 h. Although fentanyl did not affect cell proliferation, morphine exposure for 6 h enhanced the proliferation. However, sensitivity of HCT116 cells to 5-fluorouracil was not altered in all treatment groups. The current study demonstrated that the opioids commonly used during postoperative periods do not affect 5-fluorouracil sensitivity in human colon cancer HCT116 cells.
authors.affiliation: Department of Anesthesiology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara, Japan.
authors.forename: Yasumitsu
authors.initials: Y
authors.lastname: Nomura
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Kawaraguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugimoto
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Furuya
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kawaguchi
mesh_terms: D000701:Analgesics, Opioid / Q000494:pharmacology*; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy*; D004347:Drug Interactions; D005283:Fentanyl / Q000494:pharmacology*; D005472:Fluorouracil / Q000494:pharmacology*; D045325:HCT116 Cells; D006801:Humans; D009020:Morphine / Q000494:pharmacology*
chemical_list: D000701:Analgesics, Opioid; D000964:Antimetabolites, Antineoplastic; D009020:Morphine; D005472:Fluorouracil; D005283:Fentanyl