title: Proteomic analysis of the excretory-secretory products from larval stages of Ascaris suum reveals high abundance of glycosyl hydrolases.
abstract: BACKGROUND
  Ascaris lumbricoides and Ascaris suum are socioeconomically important and widespread parasites of humans and pigs, respectively. The excretory-secretory (ES) molecules produced and presented at the parasite-host interface during the different phases of tissue invasion and migration are likely to play critical roles in the induction and development of protective immune and other host responses.
  
  
  RESULTS
  The aim of this study was to identify the ES proteins of the different larval stages (L3-egg, L3-lung and L4) by LC-MS/MS. In total, 106 different proteins were identified, 20 in L3-egg, 45 in L3-lung stage and 58 in L4. Although most of the proteins identified were stage-specific, 15 were identified in the ES products of at least two stages. Two proteins, i.e. a 14-3-3-like protein and a serpin-like protein, were present in the ES products from the three different larval stages investigated. Interestingly, a comparison of ES products from L4 with those of L3-egg and L3-lung showed an abundance of metabolic enzymes, particularly glycosyl hydrolases. Further study indicated that most of these glycolytic enzymes were transcriptionally upregulated from L4 onwards, with a peak in the adult stage, particularly in intestinal tissue. This was also confirmed by enzymatic assays, showing the highest glycosidase activity in protein extracts from adult worms gut.
  
  
  CONCLUSIONS
  The present proteomic analysis provides important information on the host-parasite interaction and the biology of the migratory stages of A. suum. In particular, the high transcriptional upregulation of glycosyl hydrolases from the L4 stage onwards reveals that the degradation of complex carbohydrates forms an essential part of the energy metabolism of this parasite once it establishes in the small intestine.
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Katleen
authors.initials: K
authors.lastname: Van Steendam
authors.forename: Maarten
authors.initials: M
authors.lastname: Dhaenens
authors.forename: Johnny
authors.initials: J
authors.lastname: Vlaminck
authors.forename: Dieter
authors.initials: D
authors.lastname: Deforce
authors.forename: Aaron R
authors.initials: AR
authors.lastname: Jex
authors.forename: Robin B
authors.initials: RB
authors.lastname: Gasser
authors.forename: Peter
authors.initials: P
authors.lastname: Geldhof
mesh_terms: D000818:Animals; D017165:Ascaris suum / Q000737:chemistry*; D002853:Chromatography, Liquid; D006026:Glycoside Hydrolases / Q000032:analysis* / Q000378:metabolism*; D015801:Helminth Proteins / Q000032:analysis* / Q000378:metabolism*; D007814:Larva / Q000737:chemistry; D020543:Proteome / Q000032:analysis*; D013552:Swine; D053719:Tandem Mass Spectrometry
chemical_list: D015801:Helminth Proteins; D020543:Proteome; D006026:Glycoside Hydrolases