title: Interplay between polymerase II- and polymerase III-assisted expression of overlapping genes.
abstract: Up to 15% of the genes in different genomes overlap. This architecture, although beneficial for the genome size, represents an obstacle for simultaneous transcription of both genes. Here we analyze the interference between RNA-polymerase II (Pol II) and RNA-polymerase III (Pol III) when transcribing their target genes encoded on opposing strands within the same DNA fragment in Arabidopsis thaliana. The expression of a Pol II-dependent protein-coding gene negatively correlated with the transcription of a Pol III-dependent, tRNA-coding gene set. We suggest that the architecture of the overlapping genes introduces an additional layer of control of gene expression.
authors.affiliation: Department of Biochemistry, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam, Germany; Department of Molecular Biology, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam, Germany.
authors.forename: Radoslaw
authors.initials: R
authors.lastname: Lukoszek
authors.forename: Bernd
authors.initials: B
authors.lastname: Mueller-Roeber
authors.forename: Zoya
authors.initials: Z
authors.lastname: Ignatova
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D012319:RNA Polymerase II / Q000378:metabolism*; D012320:RNA Polymerase III / Q000378:metabolism*; D012361:RNA, Transfer, Pro / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; D012361:RNA, Transfer, Pro; D012319:RNA Polymerase II; D012320:RNA Polymerase III
keywords: Arabidopsis thaliana; Gene expression; Nested and overlapping genes; Transcription; tRNA