title: Enhanced doxorubicin delivery and cytotoxicity in multidrug resistant cancer cells using multifunctional magnetic nanoparticles.
abstract: Carboxymethyl modified magnetic nanoparticles (CMC-MNPs) have been designed as a vehicle for drug delivery in both drug-sensitive and drug-resistant cancer cells. We have demonstrated that the CMC-MNPs were able to load doxorubicin (DOX) with a high loading efficiency while also maintaining a good colloidal stability in an aqueous solution. According to a drug release study, DOX-loaded CMC-MNPs showed that the pH-dependent drug release property had a much higher release rate in acidic pH. Compared to free DOX, the DOX-loaded CMC-MNPs showed higher DOX accumulation in drug-sensitive cancer cells and much higher accumulation in drug-resistant cancer cells. These results indicate that our nanoplatform is highly efficient as a drug delivery system in both normal cancer cells and MDR cancer cells. In addition, the DOX-loaded CMC-MNPs can also enhance cytotoxicity against drug-resistant cancer cells in comparison to free DOX. The results obtained in this research demonstrate that our nanoplatform may be a promising approach in cancer chemotherapy and for overcoming multidrug-resistant cancer cells.
authors.affiliation: Center of Excellence for Molecular Imaging (CEMI), Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: chalermchai.pilapong@cmu.ac.th.
authors.forename: Chalermchai
authors.initials: C
authors.lastname: Pilapong
authors.affiliation: Department of Physics and Material Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Yanee
authors.initials: Y
authors.lastname: Keereeta
authors.affiliation: Center of Excellence for Molecular Imaging (CEMI), Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Samlee
authors.initials: S
authors.lastname: Munkhetkorn
authors.affiliation: Department of Physics and Material Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Somchai
authors.initials: S
authors.lastname: Thongtem
authors.affiliation: Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Titipun
authors.initials: T
authors.lastname: Thongtem
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry* / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry; D019008:Drug Resistance, Neoplasm; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D058185:Magnetite Nanoparticles; D004317:Doxorubicin
keywords: Doxorubicin; Magnetic nanoparticles; Multidrug resistance; pH-Dependent drug release