title: Identification of serum component involved in generation of neo-lectin with agglutinating and phenoloxidase activities in human serum.
abstract: Human serum albumin (HSA) was identified as the component involved in generation of neo-lectin molecules with both lectin and phenoloxidase activities. Pronase treated HSA was able to agglutinate hen RBC and oxidize hydroquinone. Sodium dodecyl sulphate (SDS) treated HSA agglutinated both hen and sheep RBC as well as oxidized dopamine. The hemagglutinating activities of pronase/SDS treated HSA observed against hen RBC were dosimetric. The oxidation of pronase/SDS treated HSA with hydroquinone/dopamine, respectively, was inhibitable by inhibitors of phenoloxidase, namely, phenylthiourea and tropolone. Very low concentrations of HSA could generate these humoral neo-lectin molecules.
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, India. Electronic address: beulaja@gmail.com.
authors.forename: Beulaja
authors.initials: B
authors.lastname: Manikandan
authors.affiliation: Department of Animal Health and Management, Alagappa University, Alagappapuram, Karaikudi 630 003, India.
authors.forename: Manikandan
authors.initials: M
authors.lastname: Ramar
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, India.
authors.forename: Arumugam
authors.initials: A
authors.lastname: Munusamy
mesh_terms: D000818:Animals; D002645:Chickens; D006384:Hemagglutination; D006386:Hemagglutination Tests; D006801:Humans; D037102:Lectins / Q000097:blood / Q000737:chemistry / Q000378:metabolism*; D014442:Monophenol Monooxygenase / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D010670:Phenylthiourea / Q000494:pharmacology; D011402:Pronase / Q000737:chemistry; D044967:Serum / Q000737:chemistry / Q000378:metabolism*; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism; D012756:Sheep; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013379:Substrate Specificity; D014334:Tropolone / Q000494:pharmacology
chemical_list: D037102:Lectins; D012709:Serum Albumin; D012967:Sodium Dodecyl Sulfate; D010670:Phenylthiourea; D014334:Tropolone; D014442:Monophenol Monooxygenase; D011402:Pronase