title: Antibiotic-induced autoactivation of IS256 in Staphylococcus aureus.
abstract: The 3' end of rsbU, encoding the positive regulator of the stress factor sigma B, was identified as a hot spot for spontaneous IS256 insertion in Staphylococcus aureus SA137/93G. Interestingly, subinhibitory concentrations of chloramphenicol in combination with heat stress, as well as linezolid and spectinomycin at physiological temperatures, selected for such rsbU::IS256 insertion mutants. In consequence of the inactivation of rsbU, the IS256 transposition frequency was increased 4-fold in S. aureus HG001.
authors.affiliation: Institute of Medical Microbiology, Immunology and Parasitology, University of Bonn, Bonn, Germany.
authors.forename: Franziska
authors.initials: F
authors.lastname: Schreiber
authors.forename: Christiane
authors.initials: C
authors.lastname: Szekat
authors.forename: Michaele
authors.initials: M
authors.lastname: Josten
authors.forename: Hans-Georg
authors.initials: HG
authors.lastname: Sahl
authors.forename: Gabriele
authors.initials: G
authors.lastname: Bierbaum
mesh_terms: D000081:Acetamides / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002701:Chloramphenicol / Q000494:pharmacology; D004251:DNA Transposable Elements / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000235:genetics; D000069349:Linezolid; D009154:Mutation; D023303:Oxazolidinones / Q000494:pharmacology; D000198:Spectinomycin / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics*; D013696:Temperature
chemical_list: D000081:Acetamides; D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D023303:Oxazolidinones; D002701:Chloramphenicol; D000198:Spectinomycin; D000069349:Linezolid