title: Bone marrow-derived mononuclear cells promote improvement in glomerular function in rats with early diabetic nephropathy.
abstract: OBJECTIVE
  Diabetic nephropathy is one of the main causes of end-stage renal disease. The present study investigated the effect of mononuclear cell (MC) therapy in rats subjected to diabetic nephropathy.
  
  
  METHODS
  Male Wistar rats were divided into control (CTRL), diabetic (DM), CTRL+MC and DM+MC groups. Diabetes was induced by a single injection of streptozotocin (45 mg/kg, i.p.) and, 4 weeks later, 2×10(7) MCs were injected via the jugular vein.
  
  
  RESULTS
  The rats in the DM and DM+MC groups showed increased glycemia, glomerular filtration rate and glomerular tuff area versus control groups. The glomerular filtration rate and glomerular tuff area were normalized in the DM+MC group. No alterations were observed in the fractional excretion of electrolytes and proteinuria between the DM and DM+MC groups. TGF-β1 protein levels in the DM group were significantly increased versus control animals and normalized in the DM+MC group. An increase in ED1(+)/arginase I(+) macrophages and IL-10 renal expression was observed in the DM+MC group versus DM group.
  
  
  CONCLUSIONS
  Bone marrow-derived MC therapy was able to prevent glomerular alterations and TGF-β1 protein overexpression and modulated glomerular arginase I(+) macrophage infiltration in rats subjected to early diabetic nephropathy.
authors.affiliation: Laboratório de Fisiologia Celular e Molecular, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil.
authors.forename: Raquel C
authors.initials: RC
authors.lastname: Castiglione
authors.forename: Tatiana
authors.initials: T
authors.lastname: Maron-Gutierrez
authors.forename: Carolina M L
authors.initials: CM
authors.lastname: Barbosa
authors.forename: Felipe M
authors.initials: FM
authors.lastname: Ornellas
authors.forename: André Luis
authors.initials: AL
authors.lastname: Barreira
authors.forename: Carolina B A
authors.initials: CB
authors.lastname: Dibarros
authors.forename: Andréia
authors.initials: A
authors.lastname: Vasconcelos-dos-Santos
authors.forename: Bruno Diaz
authors.initials: BD
authors.lastname: Paredes
authors.forename: Bernardo M
authors.initials: BM
authors.lastname: Pascarelli
authors.forename: Bruno L
authors.initials: BL
authors.lastname: Diaz
authors.forename: Bartira
authors.initials: B
authors.lastname: Rossi-Bergmann
authors.forename: Christina M
authors.initials: CM
authors.lastname: Takiya
authors.forename: Patricia R M
authors.initials: PR
authors.lastname: Rocco
authors.forename: Jackson
authors.initials: J
authors.lastname: Souza-Menezes
authors.forename: Marcelo M
authors.initials: MM
authors.lastname: Morales
mesh_terms: D000818:Animals; D001119:Arginase / Q000378:metabolism; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D001854:Bone Marrow Cells / Q000166:cytology*; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000473:pathology / Q000601:surgery*; D003928:Diabetic Nephropathies / Q000601:surgery*; D053331:Ectodysplasins / Q000378:metabolism; D005919:Glomerular Filtration Rate; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007668:Kidney / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000637:transplantation*; D008264:Macrophages / Q000378:metabolism; D008297:Male; D011507:Proteinuria; D051381:Rats; D017208:Rats, Wistar; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D053331:Ectodysplasins; D015850:Interleukin-6; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; D016753:Interleukin-10; D001119:Arginase; C542908:arginase I, rat