title: Characteristics of cardiac epithelium at the esophagogastric junction of a pediatric population with gastroesophageal reflux.
abstract: Cardiac mucosa (CM) of the adult, regardless its location, shares phenotypic characteristics with Barrett's epithelium, namely villin expression and a Barrett's pattern of cytokeratins 7 and 20 expression. As far as we know, the phenotypic profile of CM in children has not been studied. The objective was to evaluate the phenotypic profile of cardiac mucosa from the esophagogastric junction of children with reflux symptoms. Biopsies routinely performed at the esophagogastric junction of children submitted to upper-gastrointestinal endoscopy for complaints suggestive of reflux were retrieved from the archive and used for the purposes of this study. Biopsies were assessed for the presence of squamous epithelium, cardiac and oxyntic mucosa and intestinal metaplasia. Samples displaying both squamous and columnar epithelia were immunohistochemically evaluated for the presence of villin and sucrase-isomaltae and for the expression of cytokeratins 7 and 20. From the 42 biopsies samples retrieved, 30 had simultaneously squamous and columnar epithelia. Cardiac mucosa was present in 86.7% of the cases, and intestinal metaplasia was observed only in one (3.3%). Villin expression in cardiac mucosa was observed in 96% of the cases and a cytokeratins 7 and 20 Barrett's pattern in 73%. Sucrase-isomaltase and MUC2 were only expressed in the case with intestinal metaplasia. Cardiac mucosa was high prevalent in biopsies from the esophagogastric junction of children with reflux. As in adults, cardiac mucosa in children has an immunoprofile similar to Barrett's esophagus. For the first time, it was shown that pediatric cardiac mucosa frequently expresses villin.
authors.affiliation: Department of Gastroenterology, Instituto Português de Oncologia de Lisboa de Francisco Gentil, EPE, Lisbon, Portugal; Faculdade de Ciências da Saúde, Universidade da Beira Interior, Lisbon, Portugal.
authors.forename: A
authors.initials: A
authors.lastname: Dias Pereira
authors.forename: P Magalhães
authors.initials: PM
authors.lastname: Ramalho
authors.forename: P
authors.initials: P
authors.lastname: Chaves
mesh_terms: D000293:Adolescent; D001471:Barrett Esophagus / Q000473:pathology; D002299:Cardia / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D004943:Esophagogastric Junction / Q000737:chemistry / Q000473:pathology*; D004947:Esophagus / Q000473:pathology; D005260:Female; D005753:Gastric Mucosa / Q000737:chemistry / Q000473:pathology*; D005764:Gastroesophageal Reflux / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D053554:Keratin-20 / Q000032:analysis; D053552:Keratin-7 / Q000032:analysis; D008297:Male; D008840:Microfilament Proteins / Q000032:analysis; D055262:Mucin-2 / Q000032:analysis; D010641:Phenotype; D012189:Retrospective Studies; D013394:Sucrase-Isomaltase Complex / Q000032:analysis
chemical_list: D053554:Keratin-20; D053552:Keratin-7; C524625:MUC2 protein, human; D008840:Microfilament Proteins; D055262:Mucin-2; C026615:villin; D013394:Sucrase-Isomaltase Complex
keywords: esophagogastric junction; gastroesophageal reflux; pediatric