title: Evaluation of enzyme-linked immunosorbent assays performed on milk and serum samples for detection of neosporosis and leukosis in lactating dairy cows.
abstract: Serum and milk samples from 1229 cows on 22 Ontario dairy farms were individually tested for antibodies specific for bovine leukosis virus (BLV) and Neospora caninum by enzyme-linked immunosorbent assay (ELISA). Antibodies against BLV were present in 361 serum samples (29.4%) and 369 milk samples (30.0%). Comparing the 2 tests, agreement was almost perfect (k = 0.86; 95% CI = 0.83 to 0.90) and the proportions of samples positive were not significantly different (P = 0.56). Both tests identified the same 3 herds free of bovine leukosis virus. Antibodies against N. caninum were detected in 138 serum samples (11.2%), and 111 milk samples (9.0%). Agreement between the 2 tests was moderate (k = 0.52; 95% CI = 0.43 to 0.59). Four herds were free of neosporosis by the serum test, while 10 herds were negative by the milk test. The ELISA on milk samples facilitates sample collection to classify herds free of BLV; the milk N. caninum ELISA was less reliable in predicting herd-level infection.
authors.affiliation: Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada N1G 2W1 (Walsh, Kelton, Duffield); The California Animal Health and Food Safety Laboratory System, University of California, Davis Campus, PO Box 1770, Davis, California 95617, USA (Hietala).
authors.forename: Robert B
authors.initials: RB
authors.lastname: Walsh
authors.forename: David F
authors.initials: DF
authors.lastname: Kelton
authors.forename: Sharon K
authors.initials: SK
authors.lastname: Hietala
authors.forename: Todd F
authors.initials: TF
authors.lastname: Duffield
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D003048:Coccidiosis / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D003612:Dairying; D016583:Enzootic Bovine Leukosis / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005260:Female; D007774:Lactation / Q000502:physiology*; D001909:Leukemia Virus, Bovine / Q000276:immunology; D008892:Milk / Q000737:chemistry*; D018688:Neospora / Q000276:immunology; D009864:Ontario / Q000453:epidemiology
chemical_list: D000913:Antibodies, Protozoan; D000914:Antibodies, Viral