title: Polymyxins and analogues bind to ribosomal RNA and interfere with eukaryotic translation in vitro.
abstract: Looking for targets: while the bactericidal activity of polymyxins is attributed to changes in membrane permeation, we show that these antibiotics can bind prokaryotic and eukaryotic A-sites, domains responsible for translational decoding. Polymyxin B, colistin and analogues also hinder eukaryotic translation in vitro. These new targets and effects might be partially responsible for the plethora of adverse effects by these potent bactericidal agents.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 (USA) http://torgroup.ucsd.edu/
authors.forename: Lisa S
authors.initials: LS
authors.lastname: McCoy
authors.forename: Kade D
authors.initials: KD
authors.lastname: Roberts
authors.forename: Roger L
authors.initials: RL
authors.lastname: Nation
authors.forename: Philip E
authors.initials: PE
authors.lastname: Thompson
authors.forename: Tony
authors.initials: T
authors.lastname: Velkov
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Yitzhak
authors.initials: Y
authors.lastname: Tor
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism*; D001665:Binding Sites; D003091:Colistin / Q000737:chemistry / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D011112:Polymyxin B / Q000737:chemistry / Q000378:metabolism; D011113:Polymyxins / Q000737:chemistry* / Q000378:metabolism; D014176:Protein Biosynthesis; D012335:RNA, Ribosomal / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D005456:Fluorescent Dyes; D011113:Polymyxins; D012335:RNA, Ribosomal; D011112:Polymyxin B; D003091:Colistin
keywords: RNA; antibiotics; colistin; polymyxins; translation