title: Effect of treatment with a cationic antiviral compound on acute infection with bovine viral diarrhea virus.
abstract: Bovine viral diarrhea virus (BVDV) is a widespread bovine pathogen capable of causing disease affecting multiple body systems. Previous studies have shown 2-(2-benzimidazolyl)-5-[4-(2-imidazolino)phenyl]furan dihydrochloride (DB772) effectively prevents BVDV infection in cell culture. The aim of this project was to assess the efficacy of DB772 for the prevention of acute BVDV infection. Four calves seronegative to BVDV were treated with DB772 and another 4 calves were treated with diluent only on the same dosing schedule. Each calf was subsequently challenged intranasally with BVDV. Virus was isolated consistently from untreated calves on days 4 to 8, while treated calves remained negative by virus isolation during this period. Azotemia was exhibited by all treated calves on day 4 resulting in the euthanasia of 1 calf on day 10 and the death of another on day 13. Virus was isolated from the 2 remaining treated calves on day 14 or 21. On day 21, both remaining treated calves and all 4 untreated calves had anti-BVDV antibody titers > 1:2048. This pilot study indicates that DB772 temporarily prevented acute disease due to BVDV, but carries a significant concern of renal toxicity.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, 1500 Wire Road, Auburn University, Alabama 36849-5522, USA (Newcomer, Gard); Department of Pathobiology, College of Veterinary Medicine, 127 Sugg Laboratory, Auburn University, Alabama 36849-5516, USA (Marley, Galik, Zhang, Riddell, Givens); Department of Chemistry, Georgia State University, Atlanta, Georgia 30303, USA (Boykin, Kumar); Thompson-Bishop-Sparks State Diagnostic Laboratory, 890 Simms Road, Auburn, Alabama 36831-2209, USA (Kuhnt).
authors.forename: Benjamin W
authors.initials: BW
authors.lastname: Newcomer
authors.forename: M Shonda
authors.initials: MS
authors.lastname: Marley
authors.forename: Patricia K
authors.initials: PK
authors.lastname: Galik
authors.forename: Yijing
authors.initials: Y
authors.lastname: Zhang
authors.forename: Kay P
authors.initials: KP
authors.lastname: Riddell
authors.forename: David W
authors.initials: DW
authors.lastname: Boykin
authors.forename: Arvind
authors.initials: A
authors.lastname: Kumar
authors.forename: Leah A
authors.initials: LA
authors.lastname: Kuhnt
authors.forename: Julie A
authors.initials: JA
authors.lastname: Gard
authors.forename: M Daniel
authors.initials: MD
authors.lastname: Givens
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000998:Antiviral Agents / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology*; D001806:Blood Urea Nitrogen; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000188:drug therapy* / Q000517:prevention & control; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D005260:Female; D005663:Furans / Q000494:pharmacology*; D008297:Male; D009500:Neutralization Tests / Q000662:veterinary; D010865:Pilot Projects; D011897:Random Allocation; D014766:Viremia / Q000276:immunology / Q000662:veterinary* / Q000821:virology
chemical_list: C571880:2-(2-benzimidazolyl)-5-(4-(2-imidazolino)phenyl)furan; D000914:Antibodies, Viral; D000998:Antiviral Agents; D001562:Benzimidazoles; D005663:Furans