title: Automated fiber-type-specific cross-sectional area assessment and myonuclei counting in skeletal muscle.
abstract: Skeletal muscle is an exceptionally adaptive tissue that compromises 40% of mammalian body mass. Skeletal muscle functions in locomotion, but also plays important roles in thermogenesis and metabolic homeostasis. Thus characterizing the structural and functional properties of skeletal muscle is important in many facets of biomedical research, ranging from myopathies to rehabilitation sciences to exercise interventions aimed at improving quality of life in the face of chronic disease and aging. In this paper, we focus on automated quantification of three important morphological features of muscle: 1) muscle fiber-type composition; 2) muscle fiber-type-specific cross-sectional area, and 3) myonuclear content and location. We experimentally prove that the proposed automated image analysis approaches for fiber-type-specific assessments and automated myonuclei counting are fast, accurate, and reliable.
authors.affiliation: Division of Biomedical Informatics, Departments of Biostatistics and Computer Science, College of Public Health, University of Kentucky, Lexington, Kentucky.
authors.forename: Fujun
authors.initials: F
authors.lastname: Liu
authors.forename: Christopher S
authors.initials: CS
authors.lastname: Fry
authors.forename: Jyothi
authors.initials: J
authors.lastname: Mula
authors.forename: Janna R
authors.initials: JR
authors.lastname: Jackson
authors.forename: Jonah D
authors.initials: JD
authors.lastname: Lee
authors.forename: Charlotte A
authors.initials: CA
authors.lastname: Peterson
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
mesh_terms: D000465:Algorithms; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure*; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D018485:Muscle Fibers, Skeletal / Q000378:metabolism* / Q000648:ultrastructure*
keywords: cross-sectional area; image segmentation; muscle; myonuclei counting