title: Barcoding T cell calcium response diversity with methods for automated and accurate analysis of cell signals (MAAACS).
abstract: We introduce a series of experimental procedures enabling sensitive calcium monitoring in T cell populations by confocal video-microscopy. Tracking and post-acquisition analysis was performed using Methods for Automated and Accurate Analysis of Cell Signals (MAAACS), a fully customized program that associates a high throughput tracking algorithm, an intuitive reconnection routine and a statistical platform to provide, at a glance, the calcium barcode of a population of individual T-cells. Combined with a sensitive calcium probe, this method allowed us to unravel the heterogeneity in shape and intensity of the calcium response in T cell populations and especially in naive T cells, which display intracellular calcium oscillations upon stimulation by antigen presenting cells.
authors.affiliation: Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille University, UM2, Marseille, France ; Institut National de la Santé et de la Recherche Médicale (Inserm), U1104, Marseille, France ; Centre National de la Recherche Scientifique (CNRS) UMR7280, Marseille, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Salles
authors.forename: Cyrille
authors.initials: C
authors.lastname: Billaudeau
authors.forename: Arnauld
authors.initials: A
authors.lastname: Sergé
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Bernard
authors.forename: Marie-Claire
authors.initials: MC
authors.lastname: Phélipot
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bertaux
authors.forename: Mathieu
authors.initials: M
authors.lastname: Fallet
authors.forename: Pierre
authors.initials: P
authors.lastname: Grenot
authors.forename: Didier
authors.initials: D
authors.lastname: Marguet
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: He
authors.forename: Yannick
authors.initials: Y
authors.lastname: Hamon
mesh_terms: D000818:Animals; D019556:COS Cells; D002118:Calcium / Q000378:metabolism*; D002522:Chlorocebus aethiops; D006801:Humans; D015335:Molecular Probes; D015398:Signal Transduction; D012984:Software; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D015335:Molecular Probes; D002118:Calcium