title: Anticonvulsant effects of Fuzi total alkaloid on pentylenetetrazole-induced seizure in mice.
abstract: We investigated the anticonvulsant effect of acute Fuzi total alkaloid (FTA) in seizure induced by the GABAA-receptor antagonist pentylenetetrazole (PTZ). FTA significantly increased the seizure latency and decreased the mortality in PTZ-treated mice. Administration of PTZ increased c-Fos expression in the hippocampus, medial prefrontal cortex, and piriform cortex; and this PTZ-induced effect was inhibited by FTA in a dose-dependent manner. Furthermore, the effects of FTA on PTZ-induced seizure and c-Fos expression were reversed by the GABAA/benzodiazepine receptor-selective antagonist flumazenil. These findings suggest that the anticonvulsant effects of FTA may be related to modulation of GABAA-benzodiazepine receptor complex.
authors.affiliation: National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, China.
authors.forename: Bingjin
authors.initials: B
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Tang
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Yinuo
authors.initials: Y
authors.lastname: Wang
authors.forename: Yunong
authors.initials: Y
authors.lastname: Song
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Li
authors.forename: Ranji
authors.initials: R
authors.lastname: Cui
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000378:metabolism; D004195:Disease Models, Animal; D004224:Diterpenes; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal; D005442:Flumazenil / Q000494:pharmacology; D058787:GABA-A Receptor Antagonists; D006624:Hippocampus / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010433:Pentylenetetrazole / Q000037:antagonists & inhibitors; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D011963:Receptors, GABA-A / Q000378:metabolism*; D012640:Seizures / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D000927:Anticonvulsants; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D058787:GABA-A Receptor Antagonists; D010936:Plant Extracts; D016760:Proto-Oncogene Proteins c-fos; D011963:Receptors, GABA-A; C575009:fuzi drug herbal; D005442:Flumazenil; D010433:Pentylenetetrazole