title: Determination of ethyl glucuronide in human hair by hydrophilic interaction liquid chromatography-tandem mass spectrometry.
abstract: Ethyl glucuronide (EtG) is a direct metabolite of ethanol and has been utilized as a marker for alcohol intake. This study presents development, validation and application of a new hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) method for the analysis of EtG in human hair samples. The linearity was assessed in the range of 5-2000 pg/mg hair, with a correlation coefficient of >0.99. The method was selective and sensitive, with a limit of detection (LOD) and limit of quantitation (LOQ) of 0.05 pg/mg and 0.18 pg/mg in hair, respectively. Differently from the extraction procedures in the literature, a fast and simple liquid-liquid method was used and highest recoveries and cleanest extracts were obtained. The method was successfully applied to 30 human hair samples which were taken from those who state they consume alcohol. EtG concentrations in the hair samples of alcohol users participated in this study, ranged between 1.34 and 82.73 pg/mg. From the concentration of EtG in hair strands 20 of the 30 subjects can be considered regular moderate drinkers.
authors.affiliation: Mersin University, Advanced Technology Education, Research and Application Center, Mersin, Turkey. Electronic address: fadileyener@gmail.com.
authors.forename: Fadile
authors.initials: F
authors.lastname: Yaldiz
authors.forename: Nebile
authors.initials: N
authors.lastname: Daglioglu
authors.forename: Ahmet
authors.initials: A
authors.lastname: Hilal
authors.forename: Alper
authors.initials: A
authors.lastname: Keten
authors.forename: Mete Korkut
authors.initials: MK
authors.lastname: Gülmen
mesh_terms: D000328:Adult; D000428:Alcohol Drinking; D002853:Chromatography, Liquid; D053593:Forensic Toxicology / Q000379:methods; D005965:Glucuronates / Q000032:analysis*; D006197:Hair / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D057230:Limit of Detection; D008297:Male; D008875:Middle Aged; D053719:Tandem Mass Spectrometry
chemical_list: D005965:Glucuronates; C093924:ethyl glucuronide
keywords: Alcohol addiction; Ethyl Glucuronide; HILIC; Hair