title: The NOD2 receptor does not play a major role in the pathogenesis of Group B Streptococcus in mice.
abstract: Group B Streptococcus (GBS) capsular type III is an important agent of life-threatening invasive infections. It has been previously shown that encapsulated GBS is easily internalized by dendritic cells (DCs) and this internalization has an impact on cytokine production. The intracellular receptors or pathways underlying this response are not well understood. In this work, we investigated the role of NOD2 in the pathogenesis of GBS using a mouse model of infection. NOD2(-/-) mice showed similar levels of survival and bacteremia than control mice. Interestingly, ex vivo analysis of total spleen cells from infected animals showed that the absence of NOD2 results in reduced production of inflammatory cytokines. However this abridged inflammatory response does not seem to improve mouse survival. In conclusion, we demonstrated that NOD2 is not a crucial receptor to fight GBS infection and only partially contributes to the inflammatory response.
authors.affiliation: Laboratory of Immunology, Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada.
authors.forename: Paul
authors.initials: P
authors.lastname: Lemire
authors.forename: Cynthia
authors.initials: C
authors.lastname: Calzas
authors.forename: Mariela
authors.initials: M
authors.lastname: Segura
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D016470:Bacteremia / Q000235:genetics / Q000276:immunology; D016667:Bacterial Capsules / Q000276:immunology; D054357:Chemokine CXCL10 / Q000097:blood; D054370:Chemokine CXCL9 / Q000097:blood; D005260:Female; D007249:Inflammation / Q000276:immunology; D007371:Interferon-gamma / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D037181:Lectins, C-Type / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D053473:Nod2 Signaling Adaptor Protein / Q000235:genetics / Q000502:physiology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013290:Streptococcal Infections / Q000276:immunology* / Q000401:mortality; D013292:Streptococcus agalactiae / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D054357:Chemokine CXCL10; D054370:Chemokine CXCL9; D007371:Interferon-gamma; D015850:Interleukin-6; D037181:Lectins, C-Type; D053473:Nod2 Signaling Adaptor Protein; D014409:Tumor Necrosis Factor-alpha; D000099192:CD69 Antigens; C485433:Cxcl10 protein, mouse; C485969:Cxcl9 protein, mouse; C495945:Nod2 protein, mouse
keywords: CPS; DCs; GBS; Group B Streptococcus; Inflammatory cytokines; NLR family pyrin domain-containing protein 3; NLRP3; NLRs; NOD1; NOD2; Pathogenesis; TLR; capsular polysaccharide; dendritic cells; nod-like receptors; nucleotide-binding oligomerization domain-containing protein 1; nucleotide-binding oligomerization domain-containing protein 2; toll-like receptor