title: Detection of PCV-2 DNA in stool samples from infants vaccinated with RotaTeq®.
abstract: Rotarix® and RotaTeq® vaccines have led to a dramatic reduction in rotavirus disease worldwide. However, the detection of porcine circovirus type 1 (PCV-1) and 2 (PCV-2) DNA in these vaccines raised some safety concerns. Studies examining shedding of rotavirus in stool from rotavirus vaccine recipients have been performed but no published data exist regarding the shedding of PCV virus in stools of vaccinees. The goal of this study was to determine if PCV-1 and/or PCV-2 is shed in the feces of infants vaccinated with RotaTeq®. Using multiple PCR assays for detection of PCV DNA, we tested for PCV-1 and PCV-2 in 826 stool swab samples collected serially during the first 9 d after vaccination from 102 children vaccinated with RotaTeq®. Since the vaccine is recommended and uptake is high, we did not have samples from unvaccinated infants. A total of 235 (28.5%) samples from 59 vaccine recipients were positive for PCV-2 DNA by one or more assays used in this study. PCV-1 DNA was not detected in RotaTeq® or any of the stool swab extracts. Twenty-two of the 102 vaccine recipients (21.6%) shed RotaTeq® vaccine strain and 10 of these vaccinees (9.8%) were shedding both PCV DNA and rotavirus vaccine RNA. PCV DNA was detected up to 9 d post vaccination and was most frequently detected in the first 5 d after vaccination. This study demonstrated shedding of PCV-2 DNA by RotaTeq® vaccinees but we did not find evidence that this DNA was associated with viable PCV. Findings from this study support the continued use of current rotavirus vaccines.
authors.affiliation: Division of Viral Diseases; National Center for Immunization and Respiratory Diseases; Centers for Disease Control and Prevention; Atlanta, GA USA.
authors.forename: Mathew D
authors.initials: MD
authors.lastname: Esona
authors.affiliation: Division of Viral Diseases; National Center for Immunization and Respiratory Diseases; Centers for Disease Control and Prevention; Atlanta, GA USA.
authors.forename: Slavica
authors.initials: S
authors.lastname: Mijatovic-Rustempasic
authors.affiliation: Division of Viral Diseases; National Center for Immunization and Respiratory Diseases; Centers for Disease Control and Prevention; Atlanta, GA USA; College of Physicians & Surgeons; Columbia University; New York, NY USA.
authors.forename: Catherine
authors.initials: C
authors.lastname: Yen
authors.affiliation: Division of Viral Diseases; National Center for Immunization and Respiratory Diseases; Centers for Disease Control and Prevention; Atlanta, GA USA.
authors.forename: Umesh D
authors.initials: UD
authors.lastname: Parashar
authors.affiliation: Division of Viral Diseases; National Center for Immunization and Respiratory Diseases; Centers for Disease Control and Prevention; Atlanta, GA USA.
authors.forename: Jon R
authors.initials: JR
authors.lastname: Gentsch
authors.affiliation: Division of Viral Diseases; National Center for Immunization and Respiratory Diseases; Centers for Disease Control and Prevention; Atlanta, GA USA.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Bowen
authors.affiliation: College of Physicians & Surgeons; Columbia University; New York, NY USA.
authors.forename: Philip
authors.initials: P
authors.lastname: LaRussa
mesh_terms: D017925:Circovirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000821:virology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification; D022243:Rotavirus Vaccines / Q000008:administration & dosage* / Q000009:adverse effects*; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000009:adverse effects; D017201:Virus Shedding
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; C492535:RotaTeq; D022243:Rotavirus Vaccines; D014613:Vaccines, Attenuated
keywords: DNA; porcine circovirus; rotavirus vaccines; shedding; stool