title: Frequency of KRAS mutations in adult Korean patients with acute myeloid leukemia.
abstract: Mutation of KRAS genes occurs with a frequency of 0.5-32 % in AML. In the present study, mutations of KRAS codon 12, 13, and 61 were detected by pyrosequencing and direct sequencing in AML. Seven KRAS mutations (7/123, 5.7 %) were detected. The most common mutation was a G-to-A transition in the second base of KRAS codon 13. No mutations were detected in KRAS codon 61. Combinations of KRAS and FLT3 mutation were not found in the same patient. There was no statistically significant difference between patients with KRAS mutations and patients with wild-type KRAS in terms of sex, age, CBC at diagnosis, CD34 positivity, MPO positivity, FLT3 mutation, karyotype, progression-free survival, and overall survival, although this may be attributable to the small sample size. To our knowledge, this is the first report of the detection of KRAS mutation in Asian AML patients using pyrosequencing and direct sequencing. These two methods showed identical efficiencies in their ability to detect KRAS mutations in 84 patients.
authors.affiliation: Department of Laboratory Medicine, Gachon University Gil Medical Center, 1198 Guwol-dong, Namdong-gu, Incheon, 405-760, Korea.
authors.forename: Mi-Jung
authors.initials: MJ
authors.lastname: Park
authors.forename: Soon-Ho
authors.initials: SH
authors.lastname: Park
authors.forename: Pil-Whan
authors.initials: PW
authors.lastname: Park
authors.forename: Yiel-Hea
authors.initials: YH
authors.lastname: Seo
authors.forename: Kyung-Hee
authors.initials: KH
authors.lastname: Kim
authors.forename: Ji-Hun
authors.initials: JH
authors.lastname: Jeong
authors.forename: Moon Jin
authors.initials: MJ
authors.lastname: Kim
authors.forename: Jeong-Yeal
authors.initials: JY
authors.lastname: Ahn
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Jinny
authors.initials: J
authors.lastname: Park
authors.forename: Junshik
authors.initials: J
authors.lastname: Hong
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics; D002869:Chromosome Aberrations; D003062:Codon; D004252:DNA Mutational Analysis; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D059645:Mutation Rate; D011379:Prognosis; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D016283:Proto-Oncogene Proteins p21(ras); D056910:Republic of Korea; D055815:Young Adult; D018631:ras Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D003062:Codon; C117307:KRAS protein, human; D011518:Proto-Oncogene Proteins; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins