title: Use of cellobiohydrolase-free cellulase blends for the hydrolysis of microcrystalline cellulose and sugarcane bagasse pretreated by either ball milling or ionic liquid [Emim][Ac].
abstract: This study investigated the requirement of cellobiohydrolases (CBH) for saccharification of microcrystalline cellulose and sugarcane bagasse pretreated either by ball milling (BM) or by ionic liquid (IL) [Emim][Ac]. Hydrolysis was done using CBH-free blends of Pyrococcus horikoshii endoglucanase (EG) plus Pyrococcus furiosus β-glucosidase (EGPh/BGPf) or Optimash™ BG while Acremonium Cellulase was used as control. IL-pretreated substrates were hydrolyzed more effectively by CBH-free enzymes than were the BM-pretreated substrates. IL-treatment decreased the crystallinity and increased the specific surface area (SSA), whereas BM-treatment decreased the crystallinity without increasing the SSA. The hydrolysis of IL-treated cellulose by EGPh/BGPf showed a saccharification rate of 3.92 g/Lh and a glucose yield of 81% within 9h. These results indicate the efficiency of CBH-free enzymes for the hydrolysis of IL-treated substrates.
authors.affiliation: Federal University of Rio de Janeiro, Chemistry Institute, Av.Athos da Silveira Ramos, 149 - Centro de Tecnologia, Bloco A, Cidade Universitária, CEP 21941-909, Rio de Janeiro, RJ, Brazil.
authors.forename: Ricardo Sposina Sobral
authors.initials: RS
authors.lastname: Teixeira
authors.forename: Ayla Sant'Ana
authors.initials: AS
authors.lastname: da Silva
authors.forename: Han-Woo
authors.initials: HW
authors.lastname: Kim
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ishikawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Endo
authors.forename: Seung-Hwan
authors.initials: SH
authors.lastname: Lee
authors.forename: Elba P S
authors.initials: EP
authors.lastname: Bon
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000378:metabolism*; D003460:Crystallization; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D007093:Imidazoles / Q000494:pharmacology*; D052578:Ionic Liquids / Q000494:pharmacology*; D019711:Pyrococcus / Q000201:enzymology; D031786:Saccharum / Q000737:chemistry*; D013997:Time Factors; D014994:Xylose / Q000378:metabolism
chemical_list: D007093:Imidazoles; D052578:Ionic Liquids; D002482:Cellulose; C027433:bagasse; D014994:Xylose; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase; D005947:Glucose; C109691:microcrystalline cellulose; C518739:1-ethyl-3-methylimidazolium
keywords: Ball milling pretreatment; Cellobiohydrolase requirement; Cellulose crystallinity; Ionic liquid pretreatment; Sugarcane bagasse