title: N-acetylcysteine protects against fluoride-induced oxidative damage in primary rat hepatocytes.
abstract: Fluoride induces the overproduction of free radicals, which might in turn affect various biochemical parameters. Therefore, the aim of this study was to elucidate the role of N-acetylcysteine (NAC) in decreasing fluoride-induced oxidative stress. The fluoride intoxicated (0.002; 0.082; 0.164mmol/l) rat hepatocytes was pre-treated (60min) and simultaneously treated with NAC (1mmol/l). The resulting levels of lactate dehydrogenase (LDH), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR) and intracellular reduced glutathione (GSH) were measured along with the total antioxidant status (TAS) to determine whether NAC treatment reduced cell damage and/or the antioxidant state. These results suggest that NAC pre-treatment provides protection against fluoride-induced oxidative stress in hepatocytes.
authors.affiliation: Department of Food and Nutrition, Medical University of Silesia in Katowice, Jednosci 8, 41-200 Sosnowiec, Poland. Electronic address: kgoral@sum.edu.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Pawłowska-Góral
authors.forename: Ewa
authors.initials: E
authors.lastname: Kurzeja
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Stec
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012969:Sodium Fluoride / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D012969:Sodium Fluoride; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione; D000111:Acetylcysteine
keywords: Fluoride; Fluorosis; N-acetylcysteine; Oxidative stress; Rat hepatocytes