title: Immobilization of horseradish peroxidase on electrospun microfibrous membranes for biodegradation and adsorption of bisphenol A.
abstract: Horseradish peroxidase (HRP) from roots of horseradish (Amoracia rusticana) was successfully immobilized on novel enzyme carriers, poly(methyl methacrylate-co-ethyl acrylate) (PMMA CEA) microfibrous membranes, and used for removal of bisphenol A from water. PMMA CEA fibrous membranes (PFM) with fiber diameters of 300-500 nm, were fabricated by electrospinning. HRP was covalently immobilized on the surface of microfibers previously activated by polyethylenimine and glutaraldehyde. HRP loading reached 285 mg/g, and enzyme activity was 70% of free HRP after immobilization. Both stabilities and reusability of HRP were greatly improved after immobilization. After six repeated runs, immobilized HRP retained about 50% of its initial activity. Immobilized HRP exhibited significantly higher removal efficiency for bisphenol A (BPA) in 3h (93%) compared with free HRP (61%) and PFM alone (42%). The high BPA removal can be resulted by improvement of catalytic activity of immobilized HPR with adsorption on modified PMMA CEA support.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, PR China. Electronic address: carol.xuran@tongji.edu.cn.
authors.forename: Ran
authors.initials: R
authors.lastname: Xu
authors.forename: Chenglong
authors.initials: C
authors.lastname: Chi
authors.forename: Fengting
authors.initials: F
authors.lastname: Li
authors.forename: Bingru
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D001559:Benzhydryl Compounds / Q000302:isolation & purification*; D001673:Biodegradation, Environmental; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008567:Membranes, Artificial; D010636:Phenols / Q000302:isolation & purification*; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000302:isolation & purification; D000098826:Bisphenol A Compounds
chemical_list: D000180:Acrylic Resins; D001559:Benzhydryl Compounds; D004800:Enzymes, Immobilized; D006735:Horseradish Peroxidase; D008567:Membranes, Artificial; D010636:Phenols; D019904:Polymethyl Methacrylate; D014874:Water Pollutants, Chemical; C478961:poly(ethylacrylate); C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Degradation; Enzyme immobilization; Fibrous membrane; Horseradish peroxidase