title: Long-term live cell microscopy studies of lipid droplet fusion dynamics in adipocytes.
abstract: During the adipogenic differentiation process of mesenchymal stem cells, lipid droplets (LDs) grow slowly by transferring lipids between each other. Recent findings hint at the possibility that a fusion pore is involved. In this study, we analyze lipid transfer data obtained in long-term label-free microscopy studies in the framework of a Hagen-Poiseuille model. The data obtained show a LD fusion process in which the lipid transfer directionality depends on the size difference between LDs, whereas the respective rates depend on the size difference and additionally on the diameter of the smaller LDs. For the data analysis, the viscosity of the transferred material has to be known. We demonstrate that a viscosity-dependent molecular rotor dye can be used to measure LD viscosities in live cells. On this basis, we calculate the diameter of a putative lipid transfer channel which appears to have a direct dependence on the diameter of the smaller of the two participating LDs.
authors.affiliation: Department of Chemistry, University of Konstanz, 78457 Konstanz, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Jüngst
authors.forename: Matthias
authors.initials: M
authors.lastname: Klein
authors.forename: Andreas
authors.initials: A
authors.lastname: Zumbusch
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D002470:Cell Survival; D006801:Humans; D008055:Lipids; D008853:Microscopy; D015388:Organelles / Q000378:metabolism*; D013059:Spectrum Analysis, Raman; D013696:Temperature; D014783:Viscosity
chemical_list: D008055:Lipids
keywords: adipogenic differentiation; coherent anti-Stokes Raman scattering microscopy; fluorescence lifetime imaging