title: A microfluidic device for on-chip agarose microbead generation with ultralow reagent consumption.
abstract: Water-in-oil microdroplets offer microreactors for compartmentalized biochemical reactions with high throughput. Recently, the combination with a sol-gel switch ability, using agarose-in-oil microdroplets, has increased the range of possible applications, allowing for example the capture of amplicons in the gel phase for the preservation of monoclonality during a PCR reaction. Here, we report a new method for generating such agarose-in-oil microdroplets on a microfluidic device, with minimized inlet dead volume, on-chip cooling, and in situ monitoring of biochemical reactions within the gelified microbeads. We used a flow-focusing microchannel network and successfully generated agarose microdroplets at room temperature using the "push-pull" method. This method consists in pushing the oil continuous phase only, while suction is applied to the device outlet. The agarose phase present at the inlet is thus aspirated in the device, and segmented in microdroplets. The cooling system consists of two copper wires embedded in the microfluidic device. The transition from agarose microdroplets to microbeads provides additional stability and facilitated manipulation. We demonstrate the potential of this method by performing on-chip a temperature-triggered DNA isothermal amplification in agarose microbeads. Our device thus provides a new way to generate microbeads with high throughput and no dead volume for biochemical applications.
authors.affiliation: LIMMS/CNRS-IIS, Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.
authors.forename: Linda
authors.initials: L
authors.lastname: Desbois
authors.forename: Adrien
authors.initials: A
authors.lastname: Padirac
authors.forename: Shohei
authors.initials: S
authors.lastname: Kaneda
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Genot
authors.forename: Yannick
authors.initials: Y
authors.lastname: Rondelez
authors.forename: Didier
authors.initials: D
authors.lastname: Hober
authors.forename: Dominique
authors.initials: D
authors.lastname: Collard
authors.forename: Teruo
authors.initials: T
authors.lastname: Fujii