title: Characterization of CYP76AH4 clarifies phenolic diterpenoid biosynthesis in the Lamiaceae.
abstract: Miltiradiene (1) is the precursor of phenolic diterpenoids such as ferruginol (2), requiring aromatization and hydroxylation. While this has been attributed to a single cytochrome P450 (CYP76AH1), characterization of the rosemary ortholog CYP76AH4 led to the discovery that these CYPs simply hydroxylate the facilely oxidized aromatic intermediate abietatriene (3).
authors.affiliation: Department of Biochemistry, Biophysics & Molecular Biology, Iowa State University, Ames, IA 50011, USA. rjpeters@iastate.edu.
authors.forename: Jiachen
authors.initials: J
authors.lastname: Zi
authors.forename: Reuben J
authors.initials: RJ
authors.lastname: Peters
mesh_terms: D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004224:Diterpenes / Q000737:chemistry / Q000378:metabolism*; D019686:Lamiaceae / Q000201:enzymology / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010636:Phenols / Q000737:chemistry*
chemical_list: D004224:Diterpenes; D010636:Phenols; D003577:Cytochrome P-450 Enzyme System