title: Chlorogenic acid inhibits the replication and viability of enterovirus 71 in vitro.
abstract: Enterovirus 71 (EV71) is an etiology for a number of diseases in humans. Traditional Chinese herbs have been reported to be effective for treating EV71 infection. However, there is no report about the antiviral effects of CHA against EV71. In this study, plaque reduction assay demonstrated that the inhibitory concentration 50% (IC50) of CHA on EV71 replication is 6.3 µg/ml. When both CHA (20 µg/ml) and EV71 were added, or added post-infection at different time points, CHA was able to effectively inhibit EV71 replication between 0 and 10 h. In addition, CHA inhibited EV71 2A transcription and translation in EV71-infected RD cells, but did not affect VP1, 3C, and 3D expression. Furthermore, CHA inhibited secretions of IL-6, TNF-α, IFN-γ and MCP-1 in EV71-infected RD cells. Altogether, these results revealed that CHA may have antiviral properties for treating EV71 infection.
authors.affiliation: Department of Clinical Laboratory, The Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Xueling
authors.initials: X
authors.lastname: Hou
authors.forename: Hongjun
authors.initials: H
authors.lastname: Peng
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Jiang
authors.forename: Mei
authors.initials: M
authors.lastname: Shi
authors.forename: Yun
authors.initials: Y
authors.lastname: Ji
authors.forename: Yuyue
authors.initials: Y
authors.lastname: Wang
authors.forename: Weifeng
authors.initials: W
authors.lastname: Shi
mesh_terms: D018932:Chemokine CCL2 / Q000378:metabolism; D002726:Chlorogenic Acid / Q000737:chemistry / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D029821:Enterovirus A, Human / Q000187:drug effects*; D004769:Enterovirus Infections / Q000188:drug therapy*; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D007371:Interferon-gamma / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D050296:Microbial Viability / Q000187:drug effects*; D015394:Molecular Structure; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D002726:Chlorogenic Acid; D007371:Interferon-gamma