title: Differential active site loop conformations mediate promiscuous activities in the lactonase SsoPox.
abstract: Enzymes are proficient catalysts that enable fast rates of Michaelis-complex formation, the chemical step and products release. These different steps may require different conformational states of the active site that have distinct binding properties. Moreover, the conformational flexibility of the active site mediates alternative, promiscuous functions. Here we focused on the lactonase SsoPox from Sulfolobus solfataricus. SsoPox is a native lactonase endowed with promiscuous phosphotriesterase activity. We identified a position in the active site loop (W263) that governs its flexibility, and thereby affects the substrate specificity of the enzyme. We isolated two different sets of substitutions at position 263 that induce two distinct conformational sampling of the active loop and characterized the structural and kinetic effects of these substitutions. These sets of mutations selectively and distinctly mediate the improvement of the promiscuous phosphotriesterase and oxo-lactonase activities of SsoPox by increasing active-site loop flexibility. These observations corroborate the idea that conformational diversity governs enzymatic promiscuity and is a key feature of protein evolvability.
authors.affiliation: URMITE UMR CNRS-IRD 6236, Faculté de Médecine et de Pharmacie, Université de la Méditerranée, Marseille, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Hiblot
authors.forename: Guillaume
authors.initials: G
authors.lastname: Gotthard
authors.forename: Mikael
authors.initials: M
authors.lastname: Elias
authors.forename: Eric
authors.initials: E
authors.lastname: Chabriere
mesh_terms: D020134:Catalytic Domain / Q000235:genetics*; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D015394:Molecular Structure; D009154:Mutation / Q000235:genetics; D044345:Phosphoric Triester Hydrolases / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D013379:Substrate Specificity; D048229:Sulfolobus solfataricus / Q000201:enzymology* / Q000235:genetics
chemical_list: D006867:Hydrolases; D044345:Phosphoric Triester Hydrolases