title: Reduced hepatic mitochondrial respiration following acute high-fat diet is prevented by PGC-1α overexpression.
abstract: Changes in substrate utilization and reduced mitochondrial respiratory capacity following exposure to energy-dense, high-fat diets (HFD) are putatively key components in the development of obesity-related metabolic disease. We examined the effect of a 3-day HFD on isolated liver mitochondrial respiration and whole body energy utilization in obesity-prone (OP) rats. We also examined if hepatic overexpression of peroxisomal proliferator-activated receptor-γ coactivator-1α (PGC-1α), a master regulator of mitochondrial respiratory capacity and biogenesis, would modify liver and whole body responses to the HFD. Acute, 3-day HFD (45% kcal) in OP rats resulted in increased daily energy intake, energy balance, weight gain, and adiposity, without an increase in liver triglyceride (triacylglycerol) accumulation. HFD-fed OP rats also displayed decreased whole body substrate switching from the dark to the light cycle, which was paired with reductions in hepatic mitochondrial respiration of multiple substrates in multiple respiratory states. Hepatic PGC-1α overexpression was observed to protect whole body substrate switching, as well as maintain mitochondrial respiration, following the acute HFD. Additionally, liver PGC-1α overexpression did not alter whole body dietary fatty acid oxidation but resulted in greater storage of dietary free fatty acids in liver lipid, primarily as triacylglycerol. Together, these data demonstrate that a short-term HFD can result in a decrease in metabolic flexibility and hepatic mitochondrial respiratory capacity in OP rats that is completely prevented by hepatic overexpression of PGC-1α.
authors.affiliation: Depts. of Nutrition and Exercise Physiology and Medicine-Division of Gastroenterology and Hepatology, 1 Hospital Dr., NW502 Health Science Building, Univ. of Missouri, Columbia, MO 65202. thyfaultj@health.missouri.edu.
authors.forename: E Matthew
authors.initials: EM
authors.lastname: Morris
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Jackman
authors.forename: Grace M E
authors.initials: GM
authors.lastname: Meers
authors.forename: Ginger C
authors.initials: GC
authors.lastname: Johnson
authors.forename: Jordan L
authors.initials: JL
authors.lastname: Lopez
authors.forename: Paul S
authors.initials: PS
authors.lastname: MacLean
authors.forename: John P
authors.initials: JP
authors.lastname: Thyfault
mesh_terms: D050154:Adiposity; D000818:Animals; D019069:Cell Respiration; D059305:Diet, High-Fat / Q000009:adverse effects*; D002149:Energy Intake; D008099:Liver / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D051381:Rats; D017208:Rats, Wistar; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014280:Triglycerides / Q000378:metabolism; D015430:Weight Gain
chemical_list: D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C501429:Ppargc1a protein, rat; D014157:Transcription Factors; D014280:Triglycerides
keywords: liver; mitochondria; peroxisomal proliferator-activated receptor-γ coactivator-1α