title: Magnetic field effects on mitochondrion-activity-related optical properties in slime mold and bone forming cells.
abstract: In the present study, a cellular level response of Cyto-aa3 oxidation was investigated in real time under both time-varying and strong static magnetic fields of 5 T. Two kinds of cells, a slime mold, Physarum polycephalum, and bone forming cells, MC-3T3-E1, were used for the experiments. The oxidation level of the Cyto-aa3 was calculated by optical absorptions at 690 nm, 780 nm and 830 nm. The sample, fiber-optics and an additional optical fiber for light stimulation were set in a solenoidal coil or the bore of a 5-T superconducting magnet. The solenoidal coil for time-varying magnetic fields produced sinusoidal magnetic fields of 6 mT. The slime mold showed a periodic change in Cyto-aa3 oxidation, and the oxidation-reduction cycle of Cyto-aa3 was apparently changed when visible-light irradiated the slime mold. Similarly to the case with light, time-varying magnetic stimulations changed the oxidation-reduction cycle during and after the stimulation for 10 minutes. The same phenomena were observed in the MC-3T3-E1 cell assembly, although their cycle rhythm was comparatively random. Finally, magnetic field exposure of up to 5 T exhibited a distinct suppression of Cyto-aa3 oscillation in the bone forming cells. Exposure up to 5 T was repeated five times, and the change in Cyto-aa3 oxidation reproducibly occurred.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Mizukawa
authors.forename: Masakazu
authors.initials: M
authors.lastname: Iwasaka
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001842:Bone and Bones / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000737:chemistry*; D008027:Light; D060526:Magnetic Fields; D051379:Mice; D008928:Mitochondria / Q000502:physiology*; D055095:Optics and Photonics / Q000295:instrumentation* / Q000379:methods; D010100:Oxygen / Q000737:chemistry; D010804:Physarum / Q000378:metabolism*
chemical_list: D003576:Electron Transport Complex IV; D010100:Oxygen