title: System for stable β-estradiol-inducible gene expression in the moss Physcomitrella patens.
abstract: Inducible transgene expression provides a useful tool to analyze gene function. The moss Physcomitrellapatens is a model basal land plant with well-developed research tools, including a high efficiency of gene targeting and substantial genomics resources. However, current systems for controlled transgene expression remain limited. Here we report the development of an estrogen receptor mediated inducible gene expression system, based on the system used in flowering plants. After identifying the appropriate promoters to drive the chimeric transducer, we succeeded in inducing transcription over 1,000-fold after 24 h incubation with β-estradiol. The P. patens system was also effective for high-level long-term induction of gene expression; transcript levels of the activated gene were maintained for at least seven days on medium containing β-estradiol. We also established two potentially neutral targeting sites and a set of vectors for reproducible expression of two transgenes. This β-estradiol-dependent system will be useful to test genes individually or in combination, allowing stable, inducible transgenic expression in P. patens.
authors.affiliation: National Institute for Basic Biology, Okazaki, Japan ; ERATO, Japan Science and Technology Agency, Okazaki, Japan ; Plant Biotechnology, Faculty of Biology, University of Freiburg, Freiburg, Germany ; FRIAS - Freiburg Institute for Advanced Studies, Freiburg, Germany.
authors.forename: Minoru
authors.initials: M
authors.lastname: Kubo
authors.forename: Akihiro
authors.initials: A
authors.lastname: Imai
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Nishiyama
authors.forename: Masaki
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Sato
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kurata
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hiwatashi
authors.forename: Ralf
authors.initials: R
authors.lastname: Reski
authors.forename: Mitsuyasu
authors.initials: M
authors.lastname: Hasebe
mesh_terms: D019068:Bryopsida / Q000187:drug effects* / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D005818:Genetic Engineering / Q000379:methods*; D056426:Genetic Loci / Q000187:drug effects / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D013997:Time Factors; D015533:Transcriptional Activation / Q000187:drug effects*; D019076:Transgenes / Q000235:genetics
chemical_list: D011993:Recombinant Fusion Proteins; D004958:Estradiol