title: Isolation of an alcohol dehydrogenase cDNA from and characterization of its expression in chrysanthemum under waterlogging.
abstract: A PCR strategy was used to isolate a full-length CgADH (alcohol dehydrogenase) cDNA from chrysanthemum. The gene putatively encodes a 378 residue polypeptides, which shares 95% homology with tomato alcohol dehydrogenase class III. Endogenous ethylene generated in waterlogged Chrysanthemum zawadskii was enhanced by exogenous ethylene but decreased by 1-methylcyclopropene (1-MCP), an inhibitor of ethylene action. In waterlogged roots, the transcription of the gene encoding alcohol dehydrogenase (ADH, EC 1.1.1.1) increased rapidly but transiently, peaking at 7.5 fold the non-waterlogged level after 2h of stress. Waterlogging elevated ADH activity after a prolonged episode of stress. The exogenous supply of 40μLL(-1) ethylene suppressed the production of ethanol, while that of 4μLL(-1) 1-MCP enhanced it. Ethylene appeared to suppress an acceleration of both CgADH expression and fermentation, and alleviates ethanolic fermentation probably through by as a signal to acceleration of waterlogging-induced aerenchyma formation. This supports the previously observed phenomenon that the expression level of ADH gene is regulated by the local level of physiologically active ethylene. The relevance of the CgADH gene in relation to chrysanthemum waterlogging was discussed as well.
authors.affiliation: School of Ecological Technology and Enginneering, Shanghai Institute of Technology, Shanghai 201418, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Yin
authors.forename: Dian
authors.initials: D
authors.lastname: Ni
authors.forename: Lili
authors.initials: L
authors.lastname: Song
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D031202:Chrysanthemum / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D003521:Cyclopropanes / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014867:Water / Q000378:metabolism*
chemical_list: D003521:Cyclopropanes; D018076:DNA, Complementary; D005030:Ethylenes; D010940:Plant Proteins; D014867:Water; D000431:Ethanol; C036216:ethylene; D000426:Alcohol Dehydrogenase; C412563:1-methylcyclopropene
keywords: 1-MCP; 1-methylcyclopropene; ADH; Alcohol dehydrogenase; CgADH; Chrysanthemum; Ethylene; Gene expression; PCD; PDC; Programmed cell death; Pyruvate decarboxylase; Quantitative reverse transcription-polymerase chain reaction; Waterlogging; qRT-PCR