title: Monitoring membrane properties and apoptosis using membrane probes of the 3-hydroxyflavone family.
abstract: Environment-sensitive fluorescent membrane probes are attractive tools for investigating the membrane properties and their changes under perturbing conditions. Membrane probes of the 3-hydroxyflavone family are of particular interest due to their excited-state intramolecular proton transfer (ESIPT) reaction, which confers a dual emission highly sensitive to the polarity and hydration of the environment. In the present work, we will describe the protocols used with these probes in order to monitor the physicochemical properties of lipid membrane models and cell plasma membranes and to detect apoptosis.
authors.affiliation: Faculté de Parmacie, Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Université de Strasbourg, Illkirch, France.
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Darwich
authors.forename: Andrey S
authors.initials: AS
authors.lastname: Klymchenko
authors.forename: Yves
authors.initials: Y
authors.lastname: Mély
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D002462:Cell Membrane / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006860:Hydrogen Bonding; D008563:Membrane Lipids / Q000032:analysis; D011522:Protons; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry
chemical_list: D005419:Flavonoids; D005456:Fluorescent Dyes; D008563:Membrane Lipids; D011522:Protons; D014867:Water; C041477:3-hydroxyflavone