title: Molecular detection of adulteration in chicken products based on mitochondrial 12S rRNA gene.
abstract: The aim of this study is to detect the fraudulent in chicken products constitutes in order to protect consumers in Saudi Arabia from illegal substitutions. Two different approaches were used in this study, direct sequencing of specific fragments of amplified mitochondrial 12S rRNA gene in addition to species-specific PCR primers for confirmation of the obtained Blast search results. The results showed that all processed chicken products were identified as chicken (Gallus gallus) by 90-98% homology depending on obtained sequence quality. Samples labeled with chicken luncheon (samples tested in this study) were identified as turkey meat (Meleagris gallopavo) by 98% homology, suggesting adulteration with inedible parts of turkey in chicken luncheon ingredients. The results showed also that not only chicken luncheon was mixed with inedible parts of turkey but also all chicken products tested in this study (chicken balls, chicken burger, chicken sausage and chicken mined meat) contained this turkey meat. Applying methods used in this study could be useful for accurate and rapid identification of commercial processed meat.
authors.affiliation: Biological Sciences Department, Faculty of Sciences, King Abdul Aziz University , Jeddah , Kingdom of Saudi Arabia and.
authors.forename: Osama H A
authors.initials: OH
authors.lastname: Abuzinadah
authors.forename: Haitham Ahmed
authors.initials: HA
authors.lastname: Yacoub
authors.forename: Hassan M
authors.initials: HM
authors.lastname: El Ashmaoui
authors.forename: Hassan A I
authors.initials: HA
authors.lastname: Ramadan
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics*; D017931:DNA Primers / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D008460:Meat / Q000032:analysis; D008928:Mitochondria / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000032:analysis* / Q000235:genetics; D017423:Sequence Analysis, RNA; D013045:Species Specificity; D014422:Turkeys / Q000235:genetics
chemical_list: D017931:DNA Primers; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S
keywords: Chicken; mitochondrial DNA; processed products; sequence analysis; specific 12S rRNA fragments