title: Characterization of the Sandfly fever Naples species complex and description of a new Karimabad species complex (genus Phlebovirus, family Bunyaviridae).
abstract: Genomic and antigenic characterization of members of the Sandfly fever Naples virus (SFNV) complex reveals the presence of five clades that differ in their geographical distribution. Saint Floris and Gordil viruses, both found in Africa, form one clade; Punique, Granada and Massilia viruses, all isolated in the western Mediterranean, constitute a second; Toscana virus, a third; SFNV isolates from Italy, Cyprus, Egypt and India form a fourth; while Tehran virus and a Serbian isolate Yu 8/76, represent a fifth. Interestingly, this last clade appears not to express the second non-structural protein ORF. Karimabad virus, previously classified as a member of the SFNV complex, and Gabek Forest virus are distinct and form a new species complex (named Karimabad) in the Phlebovirus genus. In contrast with the high reassortment frequency observed in some South American phleboviruses, the only virus of the SFNV complex with evidence of reassortment was Granada virus.
authors.affiliation: Center for Genomic Sciences, United States Army Medical Research Institute for Infectious Diseases, Frederick, MD, USA.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Palacios
authors.affiliation: Center for Biodefense and Emerging Infectious Diseases, Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Robert B
authors.initials: RB
authors.lastname: Tesh
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
authors.forename: Nazir
authors.initials: N
authors.lastname: Savji
authors.affiliation: Center for Biodefense and Emerging Infectious Diseases, Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Amelia P A
authors.initials: APA
authors.lastname: Travassos da Rosa
authors.affiliation: Center for Biodefense and Emerging Infectious Diseases, Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Hilda
authors.initials: H
authors.lastname: Guzman
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
authors.forename: Ana Valeria
authors.initials: AV
authors.lastname: Bussetti
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
authors.forename: Aaloki
authors.initials: A
authors.lastname: Desai
authors.affiliation: Center for Genomic Sciences, United States Army Medical Research Institute for Infectious Diseases, Frederick, MD, USA.
authors.forename: Jason
authors.initials: J
authors.lastname: Ladner
authors.affiliation: Centro Nacional de Microbiologia, Instituto de Salud 'Carlos III', Madrid, Spain.
authors.forename: Maripaz
authors.initials: M
authors.lastname: Sanchez-Seco
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
authors.forename: W Ian
authors.initials: WI
authors.lastname: Lipkin
mesh_terms: D006801:Humans; D008969:Molecular Sequence Data; D010217:Phlebotomus Fever / Q000821:virology*; D016856:Phlebovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D058974:Phylogeography; D012367:RNA, Viral / Q000235:genetics*; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral