title: Proteomic studies of isolated lipid droplets from bacteria, C. elegans, and mammals.
abstract: Lipid droplets (LDs) are an intracellular organelle, consisting of a neutral lipid core covered by a monolayer of phospholipids and proteins. It primarily mediates lipid storage, metabolism, and transportation. Recently, research of LDs has emerged as a rapidly developing field due to the strong linkage between ectopic lipid accumulation and metabolic syndromes. Recently, more than 30 proteomic studies of isolated LDs have identified many important LD proteins that have highlighted and have also predicted the potential biological roles of the organelle, motivating the field to develop quite rapidly. This chapter summarizes methods used in proteomic studies for three representative species reported and discusses their advantages and disadvantages. We believe that this chapter provides useful information and methods for future LD proteomic studies especially for LDs in other species.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Na
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Ding
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Huina
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhensheng
authors.initials: Z
authors.lastname: Xie
authors.forename: Fuquan
authors.initials: F
authors.lastname: Yang
authors.forename: Simon
authors.initials: S
authors.lastname: Cichello
authors.forename: Pingsheng
authors.initials: P
authors.lastname: Liu
mesh_terms: D000818:Animals; D001419:Bacteria / Q000737:chemistry / Q000378:metabolism; D017173:Caenorhabditis elegans / Q000737:chemistry / Q000378:metabolism; D002479:Inclusion Bodies / Q000737:chemistry / Q000378:metabolism*; D050356:Lipid Metabolism; D008322:Mammals / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: D010743:Phospholipids
keywords: Bacteria; C. elegans; Isolation; Lipid droplet; Mammal; Proteomics