title: Eotaxin-1 is involved in parasite clearance during chronic filarial infection.
abstract: Eosinophil migration as key feature of helminth infection is increased during infection with filarial nematodes. In a mouse model of filariasis, we investigated the role of the eosinophil-attracting chemokine Eotaxin-1 on disease outcome. BALB/c and Eotaxin-1(-/-) mice were infected with the rodent filaria Litomosoides sigmodontis, and parasitic parameters, cellular migration to the site of infection, and cellular responsiveness were investigated. We found increased parasite survival but unaffected eosinophil migration to the site of infection in Eotaxin-1(-/-) mice. Expression of CD80 and CD86 was reduced on eosinophils from Eotaxin-1(-/-) mice after in vitro TLR2 stimulation and exposure to filarial antigen, respectively, suggesting a potential reduced activation state of eosinophils in Eotaxin-1 deficient mice. We further demonstrated that macrophages from Eotaxin-1(-/-) mice produce decreased amounts of IL-6 in vitro, a cytokine found to be associated with parasite containment, suggesting possible mechanisms by which Eotaxin-1 regulates activation of inflammatory cells and thus parasite survival.
authors.affiliation: Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Gentil
authors.forename: C S
authors.initials: CS
authors.lastname: Lentz
authors.forename: R
authors.initials: R
authors.lastname: Rai
authors.forename: M
authors.initials: M
authors.lastname: Muhsin
authors.forename: A D
authors.initials: AD
authors.lastname: Kamath
authors.forename: O
authors.initials: O
authors.lastname: Mutluer
authors.forename: S
authors.initials: S
authors.lastname: Specht
authors.forename: M P
authors.initials: MP
authors.lastname: Hübner
authors.forename: A
authors.initials: A
authors.lastname: Hoerauf
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D000947:Antigens, Helminth / Q000276:immunology; D002465:Cell Movement; D002478:Cells, Cultured; D054413:Chemokine CCL11 / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D054423:Chemokine CCL24 / Q000378:metabolism; D018946:Chemokine CCL5 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004804:Eosinophils / Q000276:immunology* / Q000502:physiology; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D005368:Filariasis / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology; D005370:Filarioidea / Q000254:growth & development / Q000276:immunology*; D015850:Interleukin-6 / Q000378:metabolism; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008842:Microfilariae / Q000502:physiology; D059208:Parasite Load; D035422:Pleural Cavity / Q000276:immunology / Q000469:parasitology; D013154:Spleen / Q000276:immunology
chemical_list: D000947:Antigens, Helminth; C515457:Ccl11 protein, mouse; C515485:Ccl24 protein, mouse; C503460:Ccl5 protein, mouse; D054413:Chemokine CCL11; D054423:Chemokine CCL24; D018946:Chemokine CCL5; D016207:Cytokines; D015850:Interleukin-6
keywords: Eotaxin-1; Litomosoides; chemokine; eosinophil; filaria; macrophage