title: Genogroup I avian picobirnavirus detected in Brazilian broiler chickens: a molecular epidemiology study.
abstract: Picobirnavirus (PBV) belongs to the family Picobirnaviridae. Picobirnaviruses contain a bisegmented dsRNA genome that is non-enveloped. A total of 85 pooled faecal samples were collected from the poultry of 37 farms from the Metropolitan Mesoregion of Belém (MMB), Pará state, Brazil. The viral RNA from each sample was analysed by PAGE and reverse transcriptase PCR (RT-PCR). For each county affected, at least one positive sample was selected, cloned and sequenced. The samples showed a positivity of 15.3 % (13/85) by PAGE and 49.4 % (42/85) by RT-PCR. Sequencing of these strains demonstrated a considerable RdRp gene heterogeneity that ranged from 56.1 to 100 % at the nucleotide level compared with prototypes of different species and water sewage, and from 50.3 to 100 % among themselves. Avian picobirnavirus (AvPBV) was detected in MMB broiler farms and showed a heterogeneous relationship with the prototypes used. This report includes what is believed to be the first gene sequencing of AvPBV in Brazilian broiler chickens.
authors.affiliation: Ministério da Agricultura, Pecuária e Abastecimento, Avenida Almirante Barroso, Número 1234, CEP: 66093032, Lanagro, Belém/Pará, Brazil.
authors.forename: René
authors.initials: R
authors.lastname: Ribeiro Silva
authors.affiliation: Seção de Virologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde. Rodovia BR-316 km 7, Sem Número, Levilândia. CEP: 67030-000, Ananindeua/Pará, Brazil.
authors.forename: Delana Andreza Melo
authors.initials: DAM
authors.lastname: Bezerra
authors.affiliation: Seção de Virologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde. Rodovia BR-316 km 7, Sem Número, Levilândia. CEP: 67030-000, Ananindeua/Pará, Brazil.
authors.forename: Jane Haruko Lima
authors.initials: JHL
authors.lastname: Kaiano
authors.affiliation: Seção de Virologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde. Rodovia BR-316 km 7, Sem Número, Levilândia. CEP: 67030-000, Ananindeua/Pará, Brazil.
authors.forename: Darleise de Souza
authors.initials: DS
authors.lastname: Oliveira
authors.affiliation: Seção de Virologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde. Rodovia BR-316 km 7, Sem Número, Levilândia. CEP: 67030-000, Ananindeua/Pará, Brazil.
authors.forename: Rodrigo Vellasco Duarte
authors.initials: RVD
authors.lastname: Silvestre
authors.affiliation: Seção de Virologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde. Rodovia BR-316 km 7, Sem Número, Levilândia. CEP: 67030-000, Ananindeua/Pará, Brazil.
authors.forename: Yvone Benchimol
authors.initials: YB
authors.lastname: Gabbay
authors.affiliation: Division of Virology, National Institute of Cholera and Enteric Diseases (NICED), P-33, C.I.T. Road, Scheme-XM, Beliaghata, Kolkata 700 010, West Bengal, India.
authors.forename: Balasubramanian
authors.initials: B
authors.lastname: Ganesh
authors.affiliation: Seção de Virologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde. Rodovia BR-316 km 7, Sem Número, Levilândia. CEP: 67030-000, Ananindeua/Pará, Brazil.
authors.forename: Joana D'Arc Pereira
authors.initials: JDP
authors.lastname: Mascarenhas
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002645:Chickens; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D005838:Genotype; D017720:Molecular Epidemiology; D010802:Phylogeny; D018708:Picobirnavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D011201:Poultry Diseases / Q000453:epidemiology* / Q000821:virology*; D012327:RNA Virus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology