title: Transcriptional profiles of Rel/NF-κB, inhibitor of NF-κB (IκB), and lipopolysaccharide-induced TNF-α factor (LITAF) in the lipopolysaccharide (LPS) and two Vibrio sp.-exposed intertidal copepod, Tigriopus japonicus.
abstract: The immune system and the role of immunity-related genes have rarely been studied in copepods, even though copepods have a primitive immune response system and also have a potential in pathogen transport higher trophic levels. In this study, we firstly cloned and characterized three core immune genes such as nuclear factor κB (NF-κB), inhibitor of NF-κB (IκB), and lipopolysaccharide-induced TNF-α factor (LITAF) genes in the intertidal copepod Tigriopus japonicus. Several in silico analyses based on conserved domains, motifs, and phylogenetic relationships were supporting their annotations. To investigate the immune-related role of three genes, we exposed lipopolysaccharide (LPS) and two Vibrio sp. to T. japonicus. After exposure of different concentrations of LPS and two Vibrio sp., transcripts of TJ-IκB and TJ-LITAF genes were significantly elevated during the time course in a dose-dependent manner, while TJ-NF-κB transcripts were not significantly changed during exposure. These findings demonstrated that the copepod T. japonicus has a conserved immunity against infection.
authors.affiliation: Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 133-791, South Korea.
authors.forename: Bo-Mi
authors.initials: BM
authors.lastname: Kim
authors.forename: Chang-Bum
authors.initials: CB
authors.lastname: Jeong
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Rhee
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D033342:Copepoda / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D005260:Female; D051550:I-kappa B Kinase / Q000235:genetics* / Q000276:immunology; D008070:Lipopolysaccharides; D008297:Male; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000235:genetics* / Q000276:immunology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014735:Vibrio Infections / Q000276:immunology*; D014736:Vibrio parahaemolyticus / Q000276:immunology; D041261:Vibrio vulnificus / Q000276:immunology
chemical_list: D008070:Lipopolysaccharides; D016328:NF-kappa B; D051550:I-kappa B Kinase
keywords: Copepod; IκB; LITAF; Lipopolysaccharide; NF-κB; Tigriopus japonicus