title: Measurement of calibration constants for quantitative calcium fluorimetry.
abstract: A careful calibration of the fluorescence setup is essential when using Fura-2 for quantitative fluorimetry. The calibration procedure described here is used for experiments in which the indicator dye is introduced into the cell through a patch-clamp pipette. The fluorescence ratio is determined in whole-cell recordings for three different calibration parameters that reflect different [Ca(2+)]: very high (R1), intermediate (Keff), and zero (R0).
authors.forename: Erwin
authors.initials: E
authors.lastname: Neher
mesh_terms: D002118:Calcium / Q000032:analysis*; D002138:Calibration; D003584:Cytological Techniques / Q000379:methods* / Q000592:standards*; D005470:Fluorometry / Q000379:methods* / Q000592:standards*; D016257:Fura-2 / Q000378:metabolism
chemical_list: D002118:Calcium; D016257:Fura-2