title: Investigating the ruthenium metalation of proteins: X-ray structure and Raman microspectroscopy of the complex between RNase A and AziRu.
abstract: A Raman-assisted crystallographic study on the adduct between AziRu, a Ru(III) complex with high antiproliferative activity, and RNase A is presented. The protein structure is not perturbed significantly by the Ru label. The metal coordinates to ND atoms of His105 or of His119 imidazole rings, losing all of its original ligands but retaining octahedral, although distorted, coordination geometry. The AziRu binding inactivates the enzyme, suggesting that its antitumor action can be exerted by a mechanism of competitive inhibition.
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II , Napoli, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Vergara
authors.forename: Irene
authors.initials: I
authors.lastname: Russo Krauss
authors.forename: Daniela
authors.initials: D
authors.lastname: Montesarchio
authors.forename: Luigi
authors.initials: L
authors.lastname: Paduano
authors.forename: Antonello
authors.initials: A
authors.lastname: Merlino
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry; D056831:Coordination Complexes / Q000737:chemistry*; D018360:Crystallography, X-Ray; D015394:Molecular Structure; D011506:Proteins / Q000737:chemistry*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D011506:Proteins; D012428:Ruthenium; D012259:Ribonuclease, Pancreatic