title: A recombinant pseudotyped lentivirus expressing the envelope glycoprotein of hantaan virus induced protective immunity in mice.
abstract: BACKGROUND
  Hantaviruses cause acute hemorrhagic fever with renal syndrome (HFRS). Currently, several types of inactivated HFRS vaccines are widely used, however the limited ability of these immunogen to elicit neutralizing antibodies restricts vaccine efficacy. Development of an effective vaccine to overcome this weakness is must.
  
  
  METHODS
  In the present study, a recombinant pseudotyped lentivirus bearing the hantaan virus (HTNV) envelope glycoproteins (GP), rLV-M, was constructed. C57BL/6 mice were immunized with the rLV-M and a series of immunological assays were conducted to determine the immunogenicity of the recombinant pseudotyped lentivirus. The humoral and cell-mediated immune responses induced by rLV-M were compared with those of the inactivated HFRS vaccine.
  
  
  RESULTS
  Indirect immunofluorescence assay (IFA) showed the rLV-M expressed target proteins in HEK-293 cells. In mice, the rLV-M efficiently induced GP-specific humoral responses and protection against HTNV infection. Furthermore, the rLV-M induced higher neutralizing antibody titers than the inactivated HFRS vaccine control.
  
  
  CONCLUSIONS
  The results indicated the potential of using a pseudotyped lentivirus as a delivery vector for a hantavirus vaccine immunogen.
authors.affiliation: Department of Microbiology, Fourth Military Medical University, Xi'an, 710032, China. flzhang@fmmu.edu.cn.
authors.forename: Lan
authors.initials: L
authors.lastname: Yu
authors.forename: Wentao
authors.initials: W
authors.lastname: Bai
authors.forename: Xingan
authors.initials: X
authors.lastname: Wu
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Puyuan
authors.initials: P
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Ziyu
authors.initials: Z
authors.lastname: Liu
authors.forename: Fanglin
authors.initials: F
authors.lastname: Zhang
authors.forename: Zhikai
authors.initials: Z
authors.lastname: Xu
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D004337:Drug Carriers; D058501:Enzyme-Linked Immunospot Assay; D005260:Female; D005822:Genetic Vectors; D018063:Hantaan virus / Q000235:genetics / Q000276:immunology*; D016086:Lentivirus / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009500:Neutralization Tests; D013154:Spleen / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D016207:Cytokines; D004337:Drug Carriers; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C090197:glycoprotein G2, Hantavirus