title: Inhibition of spoiling yeasts of fruit juices through citrus extracts.
abstract: This article reports on the bioactivities of citrus extracts (citrus extract, lemon extract, and neroli) toward Saccharomyces cerevisiae, Zygosaccharomyces bailii, Zygosaccharomyces rouxii, Pichia membranifaciens, and Rhodotorula bacarum. The bioactivities of the extracts (from 10 to 100 ppm) were evaluated through a microdilution method; thereafter, citrus extracts (0 to 80 ppm) were tested in combination with either pH (3.0 to 5.0) or temperature (5 to 25°C). Finally, a confirmatory experiment was run in a commercial drink (referred to as red fruit juice) containing citrus extract (40 ppm) that was inoculated with either S. cerevisiae or Z. bailii (5 log CFU/ml) and stored at 4 and 25°C. Yeasts increased to 7 log CFU/ml (Z. bailii) or 8 log CFU/ml (S. cerevisiae) in the control at 25°C, but the citrus extract addition controlled yeast growth for at least 3 days; under refrigeration, the effect was significant for 10 days.
authors.affiliation: Department of the Science of Agriculture, Food and Environment, University of Foggia, Via Napoli 25, 71122 Foggia, Italy; or abevi@libero.it, Email: antonio.bevilacqua@unifg.it.
authors.forename: Antonio
authors.initials: A
authors.lastname: Bevilacqua
authors.forename: Barbara
authors.initials: B
authors.lastname: Speranza
authors.forename: Daniela
authors.initials: D
authors.lastname: Campaniello
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Corbo
authors.forename: Milena
authors.initials: M
authors.lastname: Sinigaglia
mesh_terms: D001628:Beverages / Q000382:microbiology*; D002957:Citrus / Q000737:chemistry*; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D005519:Food Preservation / Q000379:methods*; D005520:Food Preservatives / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010843:Pichia / Q000187:drug effects / Q000254:growth & development; D010936:Plant Extracts / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development; D013696:Temperature; D015003:Yeasts / Q000187:drug effects* / Q000254:growth & development; D020068:Zygosaccharomyces / Q000187:drug effects / Q000254:growth & development
chemical_list: D005520:Food Preservatives; D010936:Plant Extracts