title: Biotransformation of 20(S)-protopanaxadiol by Aspergillus niger AS 3.1858.
abstract: The biotransformation of 20(S)-protopanaxadiol (1) by Aspergillus niger AS 3.1858 was conducted. Seven metabolites 26-hydroxyl-20(S)-protopanaxadiol (2); 23, 24-en-25-hydroxyl-20(S)-protopanaxadiol (3); 25, 26-en-20(S)-protopanaxadiol (4); (E)-20, 22-en-25-hydroxyl-20(S)-protopanaxadiol (5); 25, 26-en-24(R)-hydroxyl-20(S)-protopanaxadiol (6); 25, 26-en-24(S)-hydroxyl-20(S)-protopanaxadiol (7); and 23, 24-en-25-ethoxyl-20(S)-protopanaxadiol (8) were afforded. Among them, 6, 7, and 8 are new compounds. The chemical structures of these metabolites were elucidated based on extensive spectral data including 2D NMR and HRMS. In addition, the cytotoxicity of substrate and all transformed products was evaluated by MTT assay using a panel of seven human tumor cell lines (Du-145, Hela, K562, K562/ADR, SH-SY5Y, HepG2, and MCF-7 cells) and one normal cell line Vero.
authors.affiliation: School of Medicine, Nantong University, 19 Qi Xiu Road, Nantong 226001, PR China.
authors.forename: Guangtong
authors.initials: G
authors.lastname: Chen
authors.affiliation: School of Medicine, Nantong University, 19 Qi Xiu Road, Nantong 226001, PR China.
authors.forename: Xue
authors.initials: X
authors.lastname: Yang
authors.affiliation: School of Medicine, Nantong University, 19 Qi Xiu Road, Nantong 226001, PR China.
authors.forename: Jianlin
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Medicine, Nantong University, 19 Qi Xiu Road, Nantong 226001, PR China.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Ge
authors.affiliation: School of Medicine, Nantong University, 19 Qi Xiu Road, Nantong 226001, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Song
authors.affiliation: School of Pharmaceutical Engineering & Life Science, Changzhou University, 1 Gehu Road, Changzhou 213164, PR China. Electronic address: renjie2006@163.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Ren
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D001234:Aspergillus niger / Q000378:metabolism*; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D001711:Biotransformation; D002522:Chlorocebus aethiops; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D015394:Molecular Structure; D009369:Neoplasms / Q000188:drug therapy*; D005894:Panax / Q000737:chemistry*; D008517:Phytotherapy; D012502:Sapogenins / Q000378:metabolism*; D014709:Vero Cells
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001688:Biological Products; D012502:Sapogenins; C062916:protopanaxadiol
keywords: 20(S)-Protopanaxadiol; Aspergillus niger AS 3.1858; Biotransformation; Cytotoxic activity