title: Comprehensive analysis of the homeodomain-leucine zipper IV transcription factor family in Cucumis sativus.
abstract: The class IV homeodomain-leucine zipper (HD-Zip IV) proteins are plant-specific transcriptional factors known to play crucial roles in plant growth and development. In this study, 11 cucumber (Cucumis sativus) HD-Zip IV genes were identified in the version 2 cucumber genome and found to be distributed unevenly across the chromosomes. The CsHDZIV (Cucumis sativus homeodomain-leucine zipper IV) gene family is smaller than in other studied species (except for rice) because of the absence of gene duplication events. Phylogenetic analysis showed that HD-Zip IV genes from cucumber, Arabidopsis, tomato, cotton, maize, and rice could be classified into five subgroups. All CsHDZIV genes appear to be derived from a basic module containing 11 exons in the coding region. Two conserved motifs of 21 and 19 nucleotides were found in the 3'-untranslated regions of six CsHDZIV genes, suggesting that post-transcriptional regulation may play a role in regulation of CsHDZIV genes. In addition, 6 of 11 CsHDZIV genes were found to undergo alternative splicing events. Reverse transcription PCR analysis showed that all CsHDZIV genes (except one) were expressed and showed preferential expression in reproductive organs.
authors.affiliation: State Key Laboratory of Crop Biology; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Huanghuai Region), Ministry of Agriculture; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, People's Republic of China.
authors.forename: Rao
authors.initials: R
authors.lastname: Fu
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Lina
authors.initials: L
authors.lastname: Wang
authors.forename: Zhonghai
authors.initials: Z
authors.lastname: Ren
mesh_terms: D020413:3' Untranslated Regions; D017398:Alternative Splicing; D032461:Chromosomes, Plant / Q000235:genetics; D018553:Cucumis sativus / Q000235:genetics*; D019143:Evolution, Molecular; D018506:Gene Expression Regulation, Plant; D005801:Genes, Homeobox; D017343:Genes, Plant; D018398:Homeodomain Proteins / Q000235:genetics*; D016350:Leucine Zippers / Q000235:genetics*; D005810:Multigene Family; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D018398:Homeodomain Proteins; D014157:Transcription Factors