title: Antibodies against benzo[a]pyrene in immunized mouse and in lung cancer patients.
abstract: OBJECTIVE
  To evaluate the production of antibodies against benzo[a]pyrene (BP) (Ab1) and corresponding antiidiotypic antibodies (Ab2) in mice after immunization with BP-protein conjugate and in lung cancer patients.
  
  
  METHODS
  The Ab1 and Ab2 levels were measured by non-competitive ELISA in blood serum of 10 mice immunized with BP-protein conjugate, and in blood serum of 288 healthy persons and 165 lung cancer patients.
  
  
  RESULTS
  The Ab1 level of was 2-fold higher than Ab2 level in blood serum of BP-immunized mice. In lung cancer patients the Ab1 level was almost 3 times higher and the Ab2 level was by 30% higher than these indexes in healthy individuals. The Ab1/Ab2 ratio was 2 in BP-immunized mice and healthy individuals and 1 in lung cancer patients.
  
  
  CONCLUSIONS
  Our data have shown that the Ab1/Ab2 ratio in lung cancer patients differ from that in healthy individuals and is close to the Ab1/Ab2 ratio in BP-immunized mouse.
authors.affiliation: Institute of Human Ecology of Siberian Branch of Russian Academy of Sciences, Kemerovo 650065, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Ustinov
authors.forename: V A
authors.initials: VA
authors.lastname: Matveeva
authors.forename: M A
authors.initials: MA
authors.lastname: Kostyanko
authors.forename: A N
authors.initials: AN
authors.lastname: Glushkov
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood* / Q000276:immunology; D000888:Antibodies, Anti-Idiotypic / Q000097:blood / Q000276:immunology; D001564:Benzo(a)pyrene / Q000009:adverse effects*; D002273:Carcinogens; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000276:immunology*; D051379:Mice
chemical_list: D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D002273:Carcinogens; D001564:Benzo(a)pyrene