title: CD24 and Nanog identify stem cells signature of ovarian epithelium and cysts that may develop to ovarian cancer.
abstract: Ovarian cancer is the most lethal gynecological cancer. There is a general debate whether ovarian cancer is an intrinsic or an imported disease. We investigated whether in normal morphological appearance and in early stages of ovarian tumorgenesis typical cancer cell markers such as CD24 and Nanog are expressed. In 25% of normal appearing ovaries of post-menopausal women there was co-localization of CD24 and Nanog in the walls of the ovarian cysts, leaving the epithelial cells on the surface of these ovaries free of Nanog or CD24 expression. In benign ovarian tumors 37% of specimens were positive to CD24 and Nanog labeling while 26% of them were localized in the cyst walls. In contrast, in serous borderline tumors 79% specimens were labeled with CD24, 42% of them were localized in cysts and in 32% of them showed co-localization with CD24 and Nanog was evident: the rest were labeled in the ovarian epithelial cells. In serous ovarian carcinomas 81% specimens were labeled with CD24 antibodies. In 45% of them co-localization with Nanog was evident in the bulk of the cancerous tissue. In mucinous carcinomas no labeling with CD24 or Nanog was evident. In view of the synergistic effect of CD24 and Nanog expressed in malignant cancer development in other systems, it is suggested that such an analysis can be valuable for early detection of ovarian cancer. Moreover, the abundance of these markers in cysts in the development of ovarian cancer may suggest that they present an intrinsic source of the development of the highly malignant disease. Finally, since CD24 is exposed on the surface of the cancer cells, it may be highly beneficial to target these cells with antibodies to CD24 conjugated to cytotoxic drugs for more efficient treatment of this malignant disease.
authors.affiliation: Department of Pathology, Wolfson Hospital, Holon 58100, Israel.
authors.forename: Letizia
authors.initials: L
authors.lastname: Schreiber
authors.affiliation: Biological Services, The Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Calanit
authors.initials: C
authors.lastname: Raanan
authors.affiliation: Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel. Electronic address: abraham.amsterdam@weizmann.ac.il.
authors.forename: Abraham
authors.initials: A
authors.lastname: Amsterdam
mesh_terms: D000368:Aged; D051927:CD24 Antigen / Q000378:metabolism*; D000077216:Carcinoma, Ovarian Epithelial; D003560:Cysts / Q000473:pathology / Q000503:physiopathology*; D004848:Epithelium / Q000503:physiopathology*; D005260:Female; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D000071317:Nanog Homeobox Protein; D009375:Neoplasms, Glandular and Epithelial / Q000503:physiopathology* / Q000648:ultrastructure; D014411:Neoplastic Stem Cells / Q000473:pathology; D010051:Ovarian Neoplasms / Q000503:physiopathology* / Q000648:ultrastructure; D010053:Ovary / Q000166:cytology / Q000503:physiopathology
chemical_list: D051927:CD24 Antigen; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein
keywords: CD24; Nanog; Ovarian cancer sub-types; Ovarian cysts