title: Salmonella modulation of host cell gene expression promotes its intracellular growth.
abstract: Salmonella Typhimurium has evolved a complex functional interface with its host cell largely determined by two type III secretion systems (T3SS), which through the delivery of bacterial effector proteins modulate a variety of cellular processes. We show here that Salmonella Typhimurium infection of epithelial cells results in a profound transcriptional reprogramming that changes over time. This response is triggered by Salmonella T3SS effector proteins, which stimulate unique signal transduction pathways leading to STAT3 activation. We found that the Salmonella-stimulated changes in host cell gene expression are required for the formation of its specialized vesicular compartment that is permissive for its intracellular replication. This study uncovers a cell-autonomous process required for Salmonella pathogenesis potentially opening up new avenues for the development of anti-infective strategies that target relevant host pathways.
authors.affiliation: Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut, United States of America.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Hannemann
authors.forename: Beile
authors.initials: B
authors.lastname: Gao
authors.forename: Jorge E
authors.initials: JE
authors.lastname: Galán
mesh_terms: D058947:Bacterial Secretion Systems / Q000502:physiology*; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D006367:HeLa Cells; D056945:Hep G2 Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000502:physiology*
chemical_list: D058947:Bacterial Secretion Systems; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human