title: [Evaluation of high resolution Luminex technology for specific HLA-DRB1* typing in the Moroccan population].
abstract: The aim of this study is to evaluate the possibility of using routinely Luminex high definition technology for the specific HLA typing of donors and recipients of hematopoietic stem cells. 340 HLA-DRB1 *, all from Moroccan individuals were first tested at the generic level and then at the specific level by PCR-SSO Luminex high definition. Alleles identified correspond to those originally found with the generic typing. The specificity could be determined only in 41.5% of cases. The percentage of specific alleles identified for DRB1 * 04 was 78.7% and varies between 17.6% and 34.6% for other alleles. Of the eight haplotypes tested, blanks obtained by PCR-SSP were all resolved. Our results confirm the reliability of the method, since we confirm the alleles identified at the generic level. Luminex high definition technology can be used for HLA-DRB1 * 04 typing, and for the resolution of ambiguities and blanks. However, it must be completed by other techniques (PCR-SSP, SBT) in the context of hematopoietic stem cells.
authors.affiliation: Unité d'immunologie, Service de transfusion sanguine et d'hémovigilance, Hôpital des enfants, CHU Ibn Sina, Rabat, Maroc.
authors.forename: Chehrazade
authors.initials: C
authors.lastname: Brick
authors.forename: Ouafa
authors.initials: O
authors.lastname: Atouf
authors.forename: Nadia
authors.initials: N
authors.lastname: Benseffaj
authors.forename: Malika
authors.initials: M
authors.lastname: Essakalli
mesh_terms: D003920:Diabetes Mellitus / Q000097:blood / Q000235:genetics; D005787:Gene Frequency; D005828:Genetics, Population / Q000295:instrumentation / Q000379:methods*; D059811:HLA-DRB1 Chains / Q000235:genetics*; D006239:Haplotypes; D006650:Histocompatibility Testing / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D009018:Morocco; D009103:Multiple Sclerosis / Q000097:blood / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014019:Tissue Donors
chemical_list: D059811:HLA-DRB1 Chains
keywords: HLA-DRB1*; Luminex HD; Morocco; PCR-SSO; specific typing