title: Down-regulation of PERK enhances resistance to ionizing radiation.
abstract: Although, ionizing radiation (IR) has been implicated to cause stress in endoplasmic reticulum (ER), how ER stress signaling and major ER stress sensors modulate cellular response to IR is unclear. Protein kinase RNA-like endoplasmic reticulum kinase (PERK) is an ER transmembrane protein which initiates unfolded protein response (UPR) or ER stress signaling when ER homeostasis is disturbed. Here, we report that down-regulation of PERK resulted in increased clonogenic survival, enhanced DNA repair and reduced apoptosis in irradiated cancer cells. Our study demonstrated that PERK has a role in sensitizing cancer cells to IR.
authors.affiliation: Centre for Cancer Research and Cell Biology, Queen's University Belfast, Belfast, Northern Ireland, United Kingdom. Electronic address: oommen1978@gmail.com.
authors.forename: Deepu
authors.initials: D
authors.lastname: Oommen
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Prise
mesh_terms: D017209:Apoptosis / Q000528:radiation effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair / Q000528:radiation effects; D015536:Down-Regulation / Q000528:radiation effects*; D020868:Gene Silencing / Q000528:radiation effects; D006801:Humans; D010766:Phosphorylation / Q000528:radiation effects; D011839:Radiation, Ionizing; D051742:Transcription Factor CHOP / Q000378:metabolism; D019892:eIF-2 Kinase / Q000378:metabolism*
chemical_list: D051742:Transcription Factor CHOP; D019892:eIF-2 Kinase
keywords: DDR; DDSBs; DNA damage; DNA damage response; DNA double strand breaks; ER-stress; IR; Ionizing radiation; PERK; SDS–PAGE; ionizing radiation; sodium dodecyl sulfate–polyacrylamide gel electrophoresis