title: Removing reprogramming roadblocks: Mbd3 depletion allows deterministic iPSC generation.
abstract: Transcription factor-induced pluripotency is extremely inefficient and assumed to be stochastic. In a recent Nature publication, Rais et al. show that depletion of the methyl-binding protein 3 yields reprogramming efficiencies of up to 100% within days, suggesting that elimination of a single gene suffices to render reprogramming a deterministic process.
authors.affiliation: Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, 185 Cambridge Street, Boston, MA 02114, USA; Harvard Stem Cell Institute, 1350 Massachusetts Avenue, Cambridge, MA 02138, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Brumbaugh
authors.forename: Konrad
authors.initials: K
authors.lastname: Hochedlinger
mesh_terms: D000818:Animals; D065150:Cellular Reprogramming / Q000502:physiology*; D005260:Female; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D008297:Male; D008954:Models, Biological