title: Study of antidepressant-like activity of an enriched phloroglucinol fraction obtained from Hypericum caprifoliatum.
abstract: BACKGROUND
  Hypericum caprifoliatum Cham & Schlecht (Guttiferae) extracts have a potential antidepressant-like effect in rodents. However, the molecular mechanisms by which these extracts exert this effect remain unclear.
  
  
  OBJECTIVE
  This study evaluated the effect of HC1, a fraction obtained from H. caprifoliatum enriched in phloroglucinol derivatives, on the Na⁺, K⁺ ATPase activity in mouse brain and verified the influence of veratrine on the effect of HC1 in the forced swimming test (FST).
  
  
  METHODS
  Veratrine (0.06 mg/kg) and HC1 (360 mg/kg) were given alone or combined i.p. 60 and p.o. 30 min, respectively, before FST. The effect of single and repeated administration (once a day for 3 consecutive days) of HC1 (360 mg/kg) on Na⁺, K⁺ ATPase activity was evaluated ex vivo in the cerebral cortex and hippocampus of mice subjected or not to FST.
  
  
  RESULTS
  HC1 reduced the immobility time (103.15 ± 18.67 s), when compared to the control group (183.6 ± 9.51 s). This effect was prevented by veratrine (151.75 ± 22.19 s). Mice repeatedly treated with HC1 presented a significant increase in Na⁺, K⁺ ATPase activity, both in cerebral cortex (46 ± 2.41 nmol Pi/min·mg protein) and hippocampus (49.83 ± 2.31 nmol Pi/min·mg protein), in relation to the respective controls (30 ± 2.66 and 29.83 ± 2.31 nmol Pi/min·mg protein respectively).
  
  
  CONCLUSIONS
  The HC1 antidepressant-like effect on FST might be related to its capacity to inhibit Na⁺ influx. HC1 increases hippocampal and cortical Na⁺, K⁺ ATPase activities possibly through long-term regulatory mechanisms.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul , Porto Alegre , Brasil and.
authors.forename: Fernanda B
authors.initials: FB
authors.lastname: Centurião
authors.forename: Andressa
authors.initials: A
authors.lastname: Braga
authors.forename: Fernanda Rossatto
authors.initials: FR
authors.lastname: Machado
authors.forename: Barbara
authors.initials: B
authors.lastname: Tagliari
authors.forename: Liz G
authors.initials: LG
authors.lastname: Müller
authors.forename: Janaína
authors.initials: J
authors.lastname: Kolling
authors.forename: Gilsane von
authors.initials: Gv
authors.lastname: Poser
authors.forename: Angela T S
authors.initials: AT
authors.lastname: Wyse
authors.forename: Stela M K
authors.initials: SM
authors.lastname: Rates
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D003863:Depression / Q000188:drug therapy*; D004195:Disease Models, Animal; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D020902:Hypericum; D008297:Male; D051379:Mice; D010696:Phloroglucinol / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013550:Swimming; D014702:Veratrine / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D000928:Antidepressive Agents; D010936:Plant Extracts; D012964:Sodium; D010696:Phloroglucinol; D000254:Sodium-Potassium-Exchanging ATPase; D014702:Veratrine