title: Characterization of thermoplastic polyurethane/polylactic acid (TPU/PLA) tissue engineering scaffolds fabricated by microcellular injection molding.
abstract: Polylactic acid (PLA) and thermoplastic polyurethane (TPU) are two kinds of biocompatible and biodegradable polymers that can be used in biomedical applications. PLA has rigid mechanical properties while TPU possesses flexible mechanical properties. Blended TPU/PLA tissue engineering scaffolds at different ratios for tunable properties were fabricated via twin screw extrusion and microcellular injection molding techniques for the first time. Multiple test methods were used to characterize these materials. Fourier transform infrared spectroscopy (FTIR) confirmed the existence of the two components in the blends; differential scanning calorimetry (DSC) and dynamic mechanical analysis (DMA) confirmed the immiscibility between the TPU and PLA. Scanning electron microscopy (SEM) images verified that, at the composition ratios studied, PLA was dispersed as spheres or islands inside the TPU matrix and that this phase morphology further influenced the scaffold's microstructure and surface roughness. The blends exhibited a large range of mechanical properties that covered several human tissue requirements. 3T3 fibroblast cell culture showed that the scaffolds supported cell proliferation and migration properly. Most importantly, this study demonstrated the feasibility of mass producing biocompatible PLA/TPU scaffolds with tunable microstructures, surface roughnesses, and mechanical properties that have the potential to be used as artificial scaffolds in multiple tissue engineering applications.
authors.affiliation: National Engineering Research Center of Novel Equipment for Polymer Processing, South China University of Technology, Guangzhou, China; Department of Mechanical Engineering, University of Wisconsin-Madison, WI, USA.
authors.forename: Hao-Yang
authors.initials: HY
authors.lastname: Mi
authors.forename: Max R
authors.initials: MR
authors.lastname: Salick
authors.forename: Xin
authors.initials: X
authors.lastname: Jing
authors.forename: Brianna R
authors.initials: BR
authors.lastname: Jacques
authors.forename: Wendy C
authors.initials: WC
authors.lastname: Crone
authors.forename: Xiang-Fang
authors.initials: XF
authors.lastname: Peng
authors.forename: Lih-Sheng
authors.initials: LS
authors.lastname: Turng
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D019344:Lactic Acid / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry*; D012212:Rheology; D013718:Tensile Strength; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D011140:Polyurethanes; D019344:Lactic Acid; C033616:poly(lactide)
keywords: Fibroblasts; Microcellular injection molding; Polylactic acid (PLA); Thermoplastic polyurethane (TPU); Tissue engineering scaffold