title: Spectroscopic investigation on interaction and sonodynamic damage of Riboflavin to DNA under ultrasonic irradiation by using Methylene Blue as fluorescent probe.
abstract: In this paper, the Riboflavin (RF) as a sonosensitizer and Methylene Blue (MB) as a fluorescent probe were used to study the interaction and sonodynamic damage to Deoxyribonucleic Acid (DNA) by fluorescence and UV-vis spectroscopy. The results showed that the RF could efficiently bind to DNA in aqueous solution and exchange with the MB through competing reaction. And then, under ultrasonic irradiation, the RF could obviously damage the DNA. In addition, the influencing factors such as ultrasonic irradiation time and RF concentration on the sonodynamic damage to DNA were also considered. The experimental results showed that the sonodynamic damage degree increase with the increase of ultrasonic irradiation time and RF concentration. Perhaps, this paper may offer some important subjects for broadening the application of RF in sonodynamic therapy (SDT) technologies for tumor treatment.
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Dandan
authors.initials: D
authors.lastname: Chen
authors.forename: Ping
authors.initials: P
authors.lastname: Fan
authors.forename: Baoxin
authors.initials: B
authors.lastname: Wang
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000378:metabolism* / Q000528:radiation effects; D004249:DNA Damage / Q000528:radiation effects; D004791:Enzyme Inhibitors / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism*; D008751:Methylene Blue / Q000378:metabolism*; D015394:Molecular Structure; D011485:Protein Binding; D011838:Radiation-Sensitizing Agents / Q000378:metabolism*; D012256:Riboflavin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014465:Ultrasonics
chemical_list: D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D011838:Radiation-Sensitizing Agents; D004247:DNA; C427203:calf thymus DNA; D008751:Methylene Blue; D012256:Riboflavin
keywords: Deoxyribonucleic acid (DNA); Methylene Blue (MB); Riboflavin (RF); Sonodynamic damage; Ultrasonic irradiation