title: Gender-specific metabolomic profiling of obesity in leptin-deficient ob/ob mice by 1H NMR spectroscopy.
abstract: Despite the numerous metabolic studies on obesity, gender bias in obesity has rarely been investigated. Here, we report the metabolomic analysis of obesity by using leptin-deficient ob/ob mice based on the gender. Metabolomic analyses of urine and serum from ob/ob mice compared with those from C57BL/6J lean mice, based on the (1)H NMR spectroscopy in combination with multivariate statistical analysis, revealed clear metabolic differences between obese and lean mice. We also identified 48 urine and 22 serum metabolites that were statistically significantly altered in obese mice compared to lean controls. These metabolites are involved in amino acid metabolism (leucine, alanine, ariginine, lysine, and methionine), tricarbocylic acid cycle and glucose metabolism (pyruvate, citrate, glycolate, acetoacetate, and acetone), lipid metabolism (cholesterol and carnitine), creatine metabolism (creatine and creatinine), and gut-microbiome-derived metabolism (choline, TMAO, hippurate, p-cresol, isobutyrate, 2-hydroxyisobutyrate, methylamine, and trigonelline). Notably, our metabolomic studies showed distinct gender variations. The obese male mice metabolism was specifically associated with insulin signaling, whereas the obese female mice metabolism was associated with lipid metabolism. Taken together, our study identifies the biomarker signature for obesity in ob/ob mice and provides biochemical insights into the metabolic alteration in obesity based on gender.
authors.affiliation: Medical Proteomics Research Center, KRIBB, Daejeon, Republic of Korea ; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea.
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Won
authors.forename: Mi-Kyung
authors.initials: MK
authors.lastname: Yoon
authors.forename: Sang-Woo
authors.initials: SW
authors.lastname: Kim
authors.forename: Youngae
authors.initials: Y
authors.lastname: Jung
authors.forename: Hyun-Whee
authors.initials: HW
authors.lastname: Bae
authors.forename: Daeyoup
authors.initials: D
authors.lastname: Lee
authors.forename: Sung Goo
authors.initials: SG
authors.lastname: Park
authors.forename: Chul-Ho
authors.initials: CH
authors.lastname: Lee
authors.forename: Geum-Sook
authors.initials: GS
authors.lastname: Hwang
authors.forename: Seung-Wook
authors.initials: SW
authors.lastname: Chi
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism / Q000652:urine; D005260:Female; D020738:Leptin / Q000172:deficiency*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055432:Metabolomics; D051379:Mice; D008820:Mice, Obese; D015999:Multivariate Analysis; D009765:Obesity / Q000097:blood / Q000378:metabolism* / Q000652:urine*; D012727:Sex Characteristics
chemical_list: D015415:Biomarkers; D020738:Leptin