title: Enhancement effect of dihydroartemisinin on human γδ T cell proliferation and killing pancreatic cancer cells.
abstract: γδ T cells play important roles in innate immunity against tumors and infections. Inhibitory effect of dihydroartemisinin on growth of cancer cells has been found in recent years. In this study, we investigated the effect of dihydroartemisinin on human γδ T cell proliferation by MTT assay and killing activity against pancreatic cancer cells SW1990, BxPC-3 and PANC-1 by LDH release assay in vitro. Intracellular molecule alterations were verified by flow cytometry. The results suggested that appropriate concentration of dihydroartemisinin favored the expansion of γδ T cells and enhanced γδ T cell mediated killing activity against pancreatic cancer cells. Up-regulation of intracellular perforin, granzyme B expression and IFN-γ production may be the important mechanism of dihydroartemisinin on increased antitumor activity of γδ T cells.
authors.affiliation: Department of Central Laboratory, 97th Hospital of PLA, 226 Tongshan Road, Xuzhou 221004, Jiangsu, China. Electronic address: zhouzhonghai298@aliyun.com.
authors.forename: Zhong-Hai
authors.initials: ZH
authors.lastname: Zhou
authors.forename: Fu-Xing
authors.initials: FX
authors.lastname: Chen
authors.forename: Wen-Rong
authors.initials: WR
authors.lastname: Xu
authors.forename: Hui
authors.initials: H
authors.lastname: Qian
authors.forename: Lei-Qing
authors.initials: LQ
authors.lastname: Sun
authors.forename: Xiao-Ting
authors.initials: XT
authors.lastname: Lü
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.forename: Hui-Chun
authors.initials: HC
authors.lastname: Ji
authors.forename: Su-Juan
authors.initials: SJ
authors.lastname: Fei
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000293:Adolescent; D000328:Adult; D000970:Antineoplastic Agents / Q000494:pharmacology*; D037621:Artemisinins / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D053804:Granzymes / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D052118:Lysosomal-Associated Membrane Protein 1 / Q000378:metabolism; D010190:Pancreatic Neoplasms; D054353:Perforin / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D055815:Young Adult
chemical_list: D000276:Adjuvants, Immunologic; D000970:Antineoplastic Agents; D037621:Artemisinins; D052118:Lysosomal-Associated Membrane Protein 1; D054353:Perforin; C039060:artenimol; D007371:Interferon-gamma; C509213:GZMB protein, human; D053804:Granzymes
keywords: Antitumor activity; Dihydroartemisinin; Pancreatic cancer; Proliferation; γδ T cell