title: Fluorescent staining of protein in sodium dodecyl sulfate polyacrylamide gels by salicylaldehyde azine.
abstract: As a non-covalent fluorescence probe, in this study, salicylaldehyde azine (SA) was introduced as a sensitive fluorescence-based dye for detecting proteins both in 1-D and 2-D polyacrylamide electrophoresis gels. Down to 0.2 ng of single protein band could be detected within 1 h, which similars to that of glutaraldehyde (GA)-silver stain, but approximately four times higher than that of SYPRO Ruby fluorescent stain. Furthermore, comparative analysis of the MS compatibility of SA stain with SYPRO Ruby stain indicated that SA stain is compatible with the downstream of protein identification by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Additionally, the probable mechanism of the SA stain was investigated by molecular docking. The results demonstrated that the interaction between SA and protein was mainly contributed by hydrogen bonding and hydrophobic forces.
authors.affiliation: Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College, Wenzhou, Zhejiang, P. R. China.
authors.forename: Mao-Wei
authors.initials: MW
authors.lastname: Ni
authors.forename: Wei-Jian
authors.initials: WJ
authors.lastname: Ye
authors.forename: Wei-Tao
authors.initials: WT
authors.lastname: Cong
authors.forename: Guo-Ying
authors.initials: GY
authors.lastname: Hong
authors.forename: Zhong-Xin
authors.initials: ZX
authors.lastname: Zhu
authors.forename: Yuan-Meng
authors.initials: YM
authors.lastname: Duan
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.forename: Li-Tai
authors.initials: LT
authors.lastname: Jin
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000447:Aldehydes / Q000737:chemistry*; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D051379:Mice; D062105:Molecular Docking Simulation; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
chemical_list: D000180:Acrylic Resins; D000447:Aldehydes; D005456:Fluorescent Dyes; D011506:Proteins; C016680:polyacrylamide gels; C013243:salicylaldehyde; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate