title: Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser.
abstract: X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses as short as 70 fs. Parametric scaling used in conventional crystallography does not account for the observed effects.
authors.affiliation: Max-Planck Institut für medizinische Forschung, Jahnstrasse 29, DE-69120 Heidelberg, Germany ; Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, DE-22607 Hamburg, Germany.
authors.forename: Lukas
authors.initials: L
authors.lastname: Lomb
authors.forename: Thomas R M
authors.initials: TR
authors.lastname: Barends
authors.forename: Stephan
authors.initials: S
authors.lastname: Kassemeyer
authors.forename: Andrew
authors.initials: A
authors.lastname: Aquila
authors.forename: Sascha W
authors.initials: SW
authors.lastname: Epp
authors.forename: Benjamin
authors.initials: B
authors.lastname: Erk
authors.forename: Lutz
authors.initials: L
authors.lastname: Foucar
authors.forename: Robert
authors.initials: R
authors.lastname: Hartmann
authors.forename: Benedikt
authors.initials: B
authors.lastname: Rudek
authors.forename: Daniel
authors.initials: D
authors.lastname: Rolles
authors.forename: Artem
authors.initials: A
authors.lastname: Rudenko
authors.forename: Robert L
authors.initials: RL
authors.lastname: Shoeman
authors.forename: Jakob
authors.initials: J
authors.lastname: Andreasson
authors.forename: Sasa
authors.initials: S
authors.lastname: Bajt
authors.forename: Miriam
authors.initials: M
authors.lastname: Barthelmess
authors.forename: Anton
authors.initials: A
authors.lastname: Barty
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Bogan
authors.forename: Christoph
authors.initials: C
authors.lastname: Bostedt
authors.forename: John D
authors.initials: JD
authors.lastname: Bozek
authors.forename: Carl
authors.initials: C
authors.lastname: Caleman
authors.forename: Ryan
authors.initials: R
authors.lastname: Coffee
authors.forename: Nicola
authors.initials: N
authors.lastname: Coppola
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Deponte
authors.forename: R Bruce
authors.initials: RB
authors.lastname: Doak
authors.forename: Tomas
authors.initials: T
authors.lastname: Ekeberg
authors.forename: Holger
authors.initials: H
authors.lastname: Fleckenstein
authors.forename: Petra
authors.initials: P
authors.lastname: Fromme
authors.forename: Maike
authors.initials: M
authors.lastname: Gebhardt
authors.forename: Heinz
authors.initials: H
authors.lastname: Graafsma
authors.forename: Lars
authors.initials: L
authors.lastname: Gumprecht
authors.forename: Christina Y
authors.initials: CY
authors.lastname: Hampton
authors.forename: Andreas
authors.initials: A
authors.lastname: Hartmann
authors.forename: Günter
authors.initials: G
authors.lastname: Hauser
authors.forename: Helmut
authors.initials: H
authors.lastname: Hirsemann
authors.forename: Peter
authors.initials: P
authors.lastname: Holl
authors.forename: James M
authors.initials: JM
authors.lastname: Holton
authors.forename: Mark S
authors.initials: MS
authors.lastname: Hunter
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kabsch
authors.forename: Nils
authors.initials: N
authors.lastname: Kimmel
authors.forename: Richard A
authors.initials: RA
authors.lastname: Kirian
authors.forename: Mengning
authors.initials: M
authors.lastname: Liang
authors.forename: Filipe R N C
authors.initials: FR
authors.lastname: Maia
authors.forename: Anton
authors.initials: A
authors.lastname: Meinhart
authors.forename: Stefano
authors.initials: S
authors.lastname: Marchesini
authors.forename: Andrew V
authors.initials: AV
authors.lastname: Martin
authors.forename: Karol
authors.initials: K
authors.lastname: Nass
authors.forename: Christian
authors.initials: C
authors.lastname: Reich
authors.forename: Joachim
authors.initials: J
authors.lastname: Schulz
authors.forename: M Marvin
authors.initials: MM
authors.lastname: Seibert
authors.forename: Raymond
authors.initials: R
authors.lastname: Sierra
authors.forename: Heike
authors.initials: H
authors.lastname: Soltau
authors.forename: John C H
authors.initials: JC
authors.lastname: Spence
authors.forename: Jan
authors.initials: J
authors.lastname: Steinbrener
authors.forename: Francesco
authors.initials: F
authors.lastname: Stellato
authors.forename: Stephan
authors.initials: S
authors.lastname: Stern
authors.forename: Nicusor
authors.initials: N
authors.lastname: Timneanu
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.forename: Georg
authors.initials: G
authors.lastname: Weidenspointner
authors.forename: Uwe
authors.initials: U
authors.lastname: Weierstall
authors.forename: Thomas A
authors.initials: TA
authors.lastname: White
authors.forename: Cornelia
authors.initials: C
authors.lastname: Wunderer
authors.forename: Henry N
authors.initials: HN
authors.lastname: Chapman
authors.forename: Joachim
authors.initials: J
authors.lastname: Ullrich
authors.forename: Lothar
authors.initials: L
authors.lastname: Strüder
authors.forename: Ilme
authors.initials: I
authors.lastname: Schlichting