title: In ovo expression of microRNA in ventral chick midbrain.
abstract: Non-coding RNAs are additional players in regulating gene expression. Targeted in ovo electroporation of specific areas provides a unique tool for spatial and temporal control of ectopic microRNA expression. However, ventral brain structures like ventral midbrain are rather difficult to reach for any manipulations. Here, we demonstrate an efficient way to electroporate miRNA into ventral midbrain using thin platinum electrodes. This method offers a reliable way to transfect specific areas of the midbrain and a useful tool for in vivo studies.
authors.affiliation: Department of Experimental Embryology, Institute of Anatomy, University of Tübingen.
authors.forename: Carola
authors.initials: C
authors.lastname: Huber
authors.forename: A Alwin Prem
authors.initials: AA
authors.lastname: Anand
authors.forename: Manfred
authors.initials: M
authors.lastname: Mauz
authors.forename: Peter
authors.initials: P
authors.lastname: Künstle
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Hupp
authors.forename: Bernhard
authors.initials: B
authors.lastname: Hirt
authors.forename: Andrea
authors.initials: A
authors.lastname: Wizenmann
mesh_terms: D000818:Animals; D002642:Chick Embryo; D018274:Electroporation / Q000295:instrumentation / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D008636:Mesencephalon / Q000196:embryology / Q000378:metabolism / Q000502:physiology*; D035683:MicroRNAs / Q000008:administration & dosage* / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection / Q000295:instrumentation / Q000379:methods*
chemical_list: D035683:MicroRNAs