title: Characterising metabolically healthy obesity in weight-discordant monozygotic twins.
abstract: OBJECTIVE
  Not all obese individuals display the metabolic disturbances commonly associated with excess fat accumulation. Mechanisms maintaining this 'metabolically healthy obesity' (MHO) are as yet unknown. We aimed to study different fat depots and transcriptional pathways in subcutaneous adipose tissue (SAT) as related to the MHO phenomenon.
  
  
  METHODS
  Sixteen rare young adult obesity-discordant monozygotic (MZ) twin pairs (intra-pair difference (∆) in BMI ≥ 3 kg/m(2)), aged 22.8-35.8 years, were examined for detailed characteristics of metabolic health (subcutaneous, intra-abdominal and liver fat [magnetic resonance imaging/spectroscopy]), OGTT, lipids, adipokines and C-reactive protein (CRP). Affymetrix U133 Plus 2.0 chips were used to analyse transcriptomics pathways related to mitochondrial function and inflammation in SAT.
  
  
  RESULTS
  Based on liver fat accumulation, two metabolically different subgroups emerged. In half (8/16) of the pairs (∆weight 17.1 ± 2.0 kg), the obese co-twin had significantly higher liver fat (∆718%), 78% increase in AUC insulin during OGTT and CRP, significantly more disturbance in the lipid profile and greater tendency for hypertension compared with the lean co-twin. In these obese co-twins, SAT expression of mitochondrial oxidative phosphorylation, branched-chain amino acid catabolism, fatty acid oxidation and adipocyte differentiation pathways were downregulated and chronic inflammation upregulated. In the other eight pairs (∆weight 17.4 ± 2.8 kg), the obese co-twin did not differ from the non-obese co-twin in liver fat (∆8%), insulin sensitivity, CRP, lipids, blood pressure or SAT transcriptomics.
  
  
  CONCLUSIONS
  Our results suggest that maintenance of high mitochondrial transcription and lack of inflammation in SAT are associated with low liver fat and MHO.
authors.affiliation: Obesity Research Unit, Research Programs Unit, Diabetes and Obesity, University of Helsinki, Biomedicum Helsinki, C424b, PO Box 63, Helsinki, Finland, 00014.
authors.forename: J
authors.initials: J
authors.lastname: Naukkarinen
authors.forename: S
authors.initials: S
authors.lastname: Heinonen
authors.forename: A
authors.initials: A
authors.lastname: Hakkarainen
authors.forename: J
authors.initials: J
authors.lastname: Lundbom
authors.forename: K
authors.initials: K
authors.lastname: Vuolteenaho
authors.forename: L
authors.initials: L
authors.lastname: Saarinen
authors.forename: S
authors.initials: S
authors.lastname: Hautaniemi
authors.forename: A
authors.initials: A
authors.lastname: Rodriguez
authors.forename: G
authors.initials: G
authors.lastname: Frühbeck
authors.forename: P
authors.initials: P
authors.lastname: Pajunen
authors.forename: T
authors.initials: T
authors.lastname: Hyötyläinen
authors.forename: M
authors.initials: M
authors.lastname: Orešič
authors.forename: E
authors.initials: E
authors.lastname: Moilanen
authors.forename: A
authors.initials: A
authors.lastname: Suomalainen
authors.forename: N
authors.initials: N
authors.lastname: Lundbom
authors.forename: J
authors.initials: J
authors.lastname: Kaprio
authors.forename: A
authors.initials: A
authors.lastname: Rissanen
authors.forename: K H
authors.initials: KH
authors.lastname: Pietiläinen
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D001835:Body Weight / Q000502:physiology*; D005260:Female; D005951:Glucose Tolerance Test; D006801:Humans; D008297:Male; D009765:Obesity / Q000097:blood / Q000378:metabolism*; D014430:Twins, Monozygotic; D055815:Young Adult