title: Lack of association between LXRα and LXRβ gene polymorphisms and prevalence of metabolic syndrome: a case-control study of an Iranian population.
abstract: The metabolic syndrome (MetS) is considered to be a major risk factor for type 2 diabetes mellitus and cardiovascular diseases. It is characterized by central adiposity, high blood pressure, glucose intolerance and abnormalities of lipoprotein metabolism. The cause of MetS is likely to be due to a complex interaction between genetic and environmental factors. Liver X receptors alpha (NR1H3) and beta (NR1H2) play a key role in lipid and carbohydrate metabolism. The aim of this study was to investigate the contribution of genetic polymorphisms in the LXRs to risk of MetS and related traits. Two common SNPs in NR1H3 (rs11039155 and rs2279238) and in NR1H2 (rs17373080 and rs2695121) were genotyped using TaqMan assays in MetS patients (n=265) and controls (n=219). Logistic regression analyses were performed to calculate the odds ratios (ORs) as a measure of association of genotypes with the presence of MetS and related phenotypes. Although The NR1H2 polymorphism rs2695121 was nominally associated with MetS but correction for multiple-testing and adjustment for age, sex and number of MetS criteria, failed to identify any significant interactions associated with prevalence of MetS. However in the haplotype analysis, a LXRα haplotype AC, was more common in controls and was associated with a significant protective effect for MetS (OR [95% CI]=0.25 [0.07-0.88], p=0.031). In conclusion, this study suggests that the above-named variants in LXRα and LXRβ genes are not potential contributors to the risk of MetS and related traits in an Iranian population.
authors.affiliation: Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Basic Science and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: rookihassan@gmail.comMajid.
authors.forename: Hassan
authors.initials: H
authors.lastname: Rooki
authors.forename: Manjid
authors.initials: M
authors.lastname: Ghayour-Mobarhan
authors.forename: Monir-Sadat
authors.initials: MS
authors.lastname: Haerian
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Ebrahimi
authors.forename: Pedram
authors.initials: P
authors.lastname: Azimzadeh
authors.forename: Alireza
authors.initials: A
authors.lastname: Heidari-Bakavoli
authors.forename: Reza
authors.initials: R
authors.lastname: Mirfakhraei
authors.forename: Shima
authors.initials: S
authors.lastname: Tavallaie
authors.forename: Reza
authors.initials: R
authors.lastname: Mirhafez
authors.forename: Gordon
authors.initials: G
authors.lastname: Ferns
authors.forename: Mohammad-Reza
authors.initials: MR
authors.lastname: Zali
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D006239:Haplotypes; D006801:Humans; D007492:Iran / Q000453:epidemiology; D015810:Linkage Disequilibrium; D000071518:Liver X Receptors; D008297:Male; D024821:Metabolic Syndrome / Q000453:epidemiology / Q000235:genetics*; D008875:Middle Aged; D057093:Orphan Nuclear Receptors / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D015995:Prevalence
chemical_list: D000071518:Liver X Receptors; C000605210:NR1H2 protein, human; C000605213:NR1H3 protein, human; D057093:Orphan Nuclear Receptors
keywords: HWE; Hardy–Weinberg equilibrium; LXRs; LXRα; LXRβ; Liver X receptors; MetS; Metabolic syndrome; SNP; Single nucleotide polymorphism; metabolic syndrome; single nucleotide polymorphism