title: Crystallization and preliminary X-ray diffraction analysis of a high-affinity phosphate-binding protein endowed with phosphatase activity from Pseudomonas aeruginosa PAO1.
abstract: In prokaryotes, phosphate starvation induces the expression of numerous phosphate-responsive genes, such as the pst operon including the high-affinity phosphate-binding protein (PBP or pstS) and alkaline phosphatases such as PhoA. This response increases the cellular inorganic phosphate import efficiency. Notably, some Pseudomonas species secrete, via a type-2 secretion system, a phosphate-binding protein dubbed LapA endowed with phosphatase activity. Here, the expression, purification, crystallization and X-ray data collection at 0.87 Å resolution of LapA are described. Combined with biochemical and enzymatic characterization, the structure of this intriguing phosphate-binding protein will help to elucidate the molecular origin of its phosphatase activity and to decipher its putative role in phosphate uptake.
authors.affiliation: Aix Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, Inserm 1095, 27 Boulevard Jean Moulin, 13385 Marseille CEDEX 5, France.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Djeghader
authors.forename: Guillaume
authors.initials: G
authors.lastname: Gotthard
authors.forename: Andrew
authors.initials: A
authors.lastname: Suh
authors.forename: Daniel
authors.initials: D
authors.lastname: Gonzalez
authors.forename: Ken
authors.initials: K
authors.lastname: Scott
authors.forename: Eric
authors.initials: E
authors.lastname: Chabriere
authors.forename: Mikael
authors.initials: M
authors.lastname: Elias
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D028044:Phosphate-Binding Proteins / Q000737:chemistry*; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry*; D010802:Phylogeny; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D028044:Phosphate-Binding Proteins; D010744:Phosphoric Monoester Hydrolases
keywords: LapA; Pseudomonas aeruginosa PAO1; phosphatase; phosphate starvation; phosphate-binding proteins; pstS