title: Prevalence and molecular characterization of G1P[8] human rotaviruses possessing DS-1-like VP6, NSP4, and NSP5/6 in Japan.
abstract: During a survey of human rotaviruses in Okayama Prefecture, Japan in the 2011-2012 rotavirus season (between September 2011 and August 2012), G3P[8] was found to be a predominant genotype overall. However, G1P[8] emerged in the latter half of the season. To clarify the genetic background of the G1P[8] strains, the VP7, VP4, VP6, NSP4, and NSP5/6 genes of the strains were sequenced and genotyped. As a result, it was demonstrated that the strains with two different genotype constellations (G1-P[8]-I1-E1-H1 and G1-P[8]-I2-E2-H2) prevailed in the season. The G1P[8] strains possessing the DS-1-like VP6, NSP4, and NSP5/6 genes (the DS-1-like G1P[8] strains), which should reveal a short electropherotype, were originated from possible intergenogroup reassortment events. The DS-1-like G1P[8] strains accounted for 74.1% of all G1P[8] strains and were detected continuously throughout the season but not in the preceding season, indicating the possibility of new introduction and rapid spreading of these strains in the 2011-2012 season. The results suggest that the intergenogroup reassortants, considered generally unstable, can spread rapidly and become relevant.
authors.affiliation: Department of Virology, Okayama Prefectural Institute for Environmental Science and Public Health, Okayama, Japan.
authors.forename: Mitsutaka
authors.initials: M
authors.lastname: Kuzuya
authors.forename: Ritsushi
authors.initials: R
authors.lastname: Fujii
authors.forename: Masako
authors.initials: M
authors.lastname: Hamano
authors.forename: Koji
authors.initials: K
authors.lastname: Kida
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Mizoguchi
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Kanadani
authors.forename: Keiko
authors.initials: K
authors.lastname: Nishimura
authors.forename: Toshio
authors.initials: T
authors.lastname: Kishimoto
mesh_terms: D000293:Adolescent; D000956:Antigens, Viral / Q000235:genetics*; D036022:Capsid Proteins / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D007564:Japan / Q000453:epidemiology; D008297:Male; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology*; D017422:Sequence Analysis, DNA; D017361:Viral Nonstructural Proteins / Q000235:genetics*
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D012367:RNA, Viral; C061332:VP6 protein, Rotavirus; D017361:Viral Nonstructural Proteins
keywords: Japan; genotype constellation; human rotavirus; intergenogroup reassortment; phylogenetic analysis