title: A peptide-mediated targeting gene delivery system for malignant glioma cells.
abstract: Glioblastoma multiforme (GBM) is the most common and malignant glioma. Although there has been considerable progress in treatment strategies, the prognosis of many patients with GBM remains poor. In this work, polyethylenimine (PEI) and the VTWTPQAWFQWV (VTW) peptide were modified and synthesized into GBM-targeting nanoparticles. The transfection efficiency of U-87 (human glioblastoma) cells was evaluated using fluorescence microscopy and flow cytometry. Cell internalization was investigated to verify the nanoparticle delivery into the cytoplasm. Results showed that the methods of polymer conjugation and the amount of VTW peptide were important factors to polymer synthesis and transfection. The PEI-VTW20 nanoparticles increased the transfection efficiency significantly. This report describes the use of VTW peptide-based PEI nanoparticles for intracellular gene delivery in a GBM cell-specific manner.
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, People's Republic of China ; Brain Science Research Institute of Shandong University, Jinan, People's Republic of China.
authors.forename: Chuanwei
authors.initials: C
authors.lastname: Wang
authors.forename: Liping
authors.initials: L
authors.lastname: Ning
authors.forename: Hongwei
authors.initials: H
authors.lastname: Wang
authors.forename: Zaijun
authors.initials: Z
authors.lastname: Lu
authors.forename: Xingang
authors.initials: X
authors.lastname: Li
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Fan
authors.forename: Xuping
authors.initials: X
authors.lastname: Wang
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004247:DNA / Q000008:administration & dosage / Q000235:genetics*; D018390:Gene Targeting / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods; D005909:Glioblastoma / Q000503:physiopathology*; D006801:Humans; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry*; D010455:Peptides / Q000493:pharmacokinetics*; D051381:Rats; D014162:Transfection / Q000379:methods*
chemical_list: D053769:Nanocapsules; D010455:Peptides; D004247:DNA
keywords: drug-delivery systems; gene transfer techniques; glioblastoma; nanoparticles; polyethylenimine