title: An effect of glycoprotein IIb/IIIa inhibitors on the kinetics of red blood cells aggregation.
abstract: The reversible aggregation of red blood cells (RBCs) continues to be of the basic science and clinical interest. Recently it has been reported about a specific binding between fibrinogen and unknown erythrocyte glycoprotein receptors. The aim of this study was to investigate whether the red blood cell aggregation (RBCA) include the cell-cell interaction using the membrane receptors that bind such ligands as fibrinogen or fibronectin. To test this hypothesis the RBCs were incubated with monafram - the drug of the monoclonal antibodies against glycoprotein (GP) IIb/IIIa, with the GPIIb-IIIa receptor antagonist tirofiban, epifibatide and with the fibrinogen inhibiting peptide. It has been found that the RBC incubation with monafram resulted in a marked RBCA decrease mainly in persons with high level of aggregation. Another research session has shown that RBC incubation with fibronectin was accompanied by a significant RBCA rise. The monafram addition to red cell incubation medium resulted in a significant RBCA lowering. The cell incubation with tirofiban and epifibatide issued in RBCA decrease. The similar results were obtained when RBCs were incubated with the fibrinogen inhibiting peptide. Although monafram, tirofiban, eptifibatide and the fibrinogen inhibiting peptide were related to fibrinogen function they didn't inhibit RBCA completely. Therefore, under moderate and low red blood cell aggregation the cell binding is probably related to nonspecific mode. It seems evident that the specific and nonspecific modes of red blood cell aggregate formation could co-exist. Additional theoretical and experimental investigations in this area are needed.
authors.affiliation: Institute of Mechanics, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: Irina A
authors.initials: IA
authors.lastname: Sokolova
authors.affiliation: Department of Medicine and Biology, Pedagogical University, Yaroslavl, Russia.
authors.forename: Alexei V
authors.initials: AV
authors.lastname: Muravyov
authors.affiliation: Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: Maria D
authors.initials: MD
authors.lastname: Khokhlova
authors.affiliation: Institute of Mechanics, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: Sofya Yu
authors.initials: SY
authors.lastname: Rikova
authors.affiliation: Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: Evgeny V
authors.initials: EV
authors.lastname: Lyubin
authors.affiliation: Institute of Mechanics, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: Marina A
authors.initials: MA
authors.lastname: Gafarova
authors.affiliation: Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: Maria N
authors.initials: MN
authors.lastname: Skryabina
authors.affiliation: Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: Angrey A
authors.initials: AA
authors.lastname: Fedyanin
authors.affiliation: Faculty of Basic Medicine, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: Darya V
authors.initials: DV
authors.lastname: Kryukova
authors.affiliation: Institute of Mechanics, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Shahnazarov
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001792:Blood Platelets / Q000166:cytology; D002450:Cell Communication; D000077542:Eptifibatide; D004903:Erythrocyte Aggregation / Q000187:drug effects*; D004912:Erythrocytes / Q000166:cytology*; D005340:Fibrinogen / Q000037:antagonists & inhibitors; D006801:Humans; D007700:Kinetics; D008024:Ligands; D055095:Optics and Photonics; D010446:Peptide Fragments / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry*; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000037:antagonists & inhibitors*; D000077466:Tirofiban; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry; D055815:Young Adult
chemical_list: D000911:Antibodies, Monoclonal; D008024:Ligands; D010446:Peptide Fragments; D010455:Peptides; D010975:Platelet Aggregation Inhibitors; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; C525918:monafram; D014443:Tyrosine; D005340:Fibrinogen; D000077466:Tirofiban; D000077542:Eptifibatide
keywords: Red blood cells; aggregation; bridging model; eptifibatide; fibrinogen; fibronectin; glycoprotein (GP) IIb/IIIa receptors; monafram; tirofiban