title: DNA adsorption characteristics of hollow spherule allophane nano-particles.
abstract: To understand the propensity of natural allophane to adsorb the DNA molecules, the adsorption characteristics were assessed against natural allophane (AK70), using single-stranded DNA (ss-DNA) and adenosine 5'-monophosphate (5'-AMP) as a reference molecule. The adsorption capacity of ss-DNA on AK70 exhibited one order of magnitude lower value as compared with that of 5'-AMP. The adsorption capacity of ss-DNA decreased with increasing pH due to the interaction generated between phosphate groups of ss-DNA and functional Al-OH groups on the wall perforations through deprotonating, associated with higher energy barrier for the adsorption of ss-DNA. The adsorption morphologies consisting of the individual ss-DNA with mono-layer coverage of the clustered allophane particle were observed successfully through transmission electron microscopy analysis.
authors.affiliation: Advanced Polymeric Nanostructured Materials Engineering, Graduate School of Engineering Toyota Technological Institute, 2-12-1 Hisakata, Tempaku, Nagoya 468-8511, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Matsuura
authors.forename: Fumitoshi
authors.initials: F
authors.lastname: Iyoda
authors.forename: Shuichi
authors.initials: S
authors.lastname: Arakawa
authors.forename: Baiju
authors.initials: B
authors.lastname: John
authors.forename: Masami
authors.initials: M
authors.lastname: Okamoto
authors.forename: Hidetomo
authors.initials: H
authors.lastname: Hayashi
mesh_terms: D000249:Adenosine Monophosphate / Q000737:chemistry; D000327:Adsorption; D000538:Aluminum Silicates / Q000737:chemistry*; D000077215:Clay; D004277:DNA, Single-Stranded / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000538:Aluminum Silicates; D004277:DNA, Single-Stranded; C049037:aluminosilicate; D000249:Adenosine Monophosphate; D000077215:Clay
keywords: Adsorption; Clustered particles; Natural allophane; Single-stranded DNA