title: The RNA-binding protein FPA regulates flg22-triggered defense responses and transcription factor activity by alternative polyadenylation.
abstract: RNA-binding proteins (RBPs) play an important role in plant host-microbe interactions. In this study, we show that the plant RBP known as FPA, which regulates 3'-end mRNA polyadenylation, negatively regulates basal resistance to bacterial pathogen Pseudomonas syringae in Arabidopsis. A custom microarray analysis reveals that flg22, a peptide derived from bacterial flagellins, induces expression of alternatively polyadenylated isoforms of mRNA encoding the defence-related transcriptional repressor ETHYLENE RESPONSE FACTOR 4 (ERF4), which is regulated by FPA. Flg22 induces expression of a novel isoform of ERF4 that lacks the ERF-associated amphiphilic repression (EAR) motif, while FPA inhibits this induction. The EAR-lacking isoform of ERF4 acts as a transcriptional activator in vivo and suppresses the flg22-dependent reactive oxygen species burst. We propose that FPA controls use of proximal polyadenylation sites of ERF4, which quantitatively limit the defence response output.
authors.affiliation: 1] RIKEN Plant Science Center, Tsurumi-ku, Yokohama, 230-0045, Japan [2] Commonwealth Scientific and Industrial Research Organization Plant Industry, Queensland Bioscience Precinct, St. Lucia, Queensland 4067, Australia.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Lyons
authors.forename: Akira
authors.initials: A
authors.lastname: Iwase
authors.forename: Thomas
authors.initials: T
authors.lastname: Gänsewig
authors.forename: Alexander
authors.initials: A
authors.lastname: Sherstnev
authors.forename: Céline
authors.initials: C
authors.lastname: Duc
authors.forename: Geoffrey J
authors.initials: GJ
authors.lastname: Barton
authors.forename: Kousuke
authors.initials: K
authors.lastname: Hanada
authors.forename: Mieko
authors.initials: M
authors.lastname: Higuchi-Takeuchi
authors.forename: Minami
authors.initials: M
authors.lastname: Matsui
authors.forename: Keiko
authors.initials: K
authors.lastname: Sugimoto
authors.forename: Kemal
authors.initials: K
authors.lastname: Kazan
authors.forename: Gordon G
authors.initials: GG
authors.lastname: Simpson
authors.forename: Ken
authors.initials: K
authors.lastname: Shirasu
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D005408:Flagellin / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant; D007113:Immunity, Innate / Q000235:genetics; D010446:Peptide Fragments / Q000494:pharmacology; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D026723:Polyadenylation / Q000235:genetics*; D044224:Pseudomonas syringae / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D029681:Arabidopsis Proteins; C505622:ERF4 protein, Arabidopsis; C432432:FPA protein, Arabidopsis; D010446:Peptide Fragments; D012333:RNA, Messenger; D018749:RNA, Plant; D016601:RNA-Binding Proteins; D017382:Reactive Oxygen Species; D012097:Repressor Proteins; D005408:Flagellin