title: Recombinant rat CC10 protein inhibits PDGF-induced airway smooth muscle cells proliferation and migration.
abstract: Abnormal migration and proliferation of airway smooth muscle cells (ASMCs) in the airway cause airway wall thickening, which is strongly related with the development of airway remodeling in asthma. Clara cell 10 kDa protein (CC10), which is secreted by the epithelial clara cells of the pulmonary airways, plays an important role in the regulation of immunological and inflammatory processes. Previous studies suggested that CC10 protein had great protective effects against inflammation in asthma. However, the effects of CC10 protein on ASMCs migration and proliferation in airway remodeling were poorly understood. In this study, we constructed the pET-22b-CC10 recombinant plasmid, induced expression and purified the recombinant rat CC10 protein from E. coli by Ni(2+) affinity chromatography and ion exchange chromatography purification. We investigated the effect of recombinant rat CC10 protein on platelet-derived growth factor (PDGF)-BB-induced ASMCs proliferation and migration. Our results demonstrated that the recombinant CC10 protein could inhibit PDGF-BB-induced cell viability, proliferation and migration. Western blot analysis showed that PDGF-BB-induced activation of cyclin D1 was inhibited by CC10. These findings implicated that CC10 could inhibit increased ASMCs proliferation, and migration induced by PDGF-BB, and this suppression effect might be associated with inhibition of cyclin D1 expression, which might offer hope for the future treatment of airway remodeling.
authors.affiliation: Laboratory of Molecular Biology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, 650 South Wanping Road, Shanghai 200030, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wei
authors.forename: Yu-Dong
authors.initials: YD
authors.lastname: Xu
authors.forename: Lei-Miao
authors.initials: LM
authors.lastname: Yin
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Ran
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.forename: Zi-Feng
authors.initials: ZF
authors.lastname: Ma
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Liu
authors.forename: Yong-Qing
authors.initials: YQ
authors.lastname: Yang
mesh_terms: D000818:Animals; D000077214:Becaplermin; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D002470:Cell Survival; D004926:Escherichia coli; D006801:Humans; D008168:Lung / Q000473:pathology; D032389:Myocytes, Smooth Muscle / Q000378:metabolism* / Q000473:pathology; D020574:Proto-Oncogene Proteins c-sis / Q000037:antagonists & inhibitors / Q000235:genetics*; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014598:Uteroglobin / Q000235:genetics* / Q000378:metabolism
chemical_list: D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins; D000077214:Becaplermin; D014598:Uteroglobin