title: Immunohistochemical study of pituitary cells in wild and captive Salminus hilarii (Characiformes: Characidae) females during the annual reproductive cycle.
abstract: Freshwater fish that live exclusively in rivers are at particular risk from fragmentation of the aquatic system, mainly the species that migrate upriver for reproduction. That is the case of Salminus hilarii, an important migratory species currently classified as "almost threatened" in the São Paulo State (Brazil), facing water pollution, dam construction, riparian habitat destruction and environmental changes that are even more serious in this State. Additionally, this species show ovulation dysfunction in captivity. Our studies focused on the identification and distribution of the pituitary cell types in the adenohypophysis of S. hilarii females, including a morphometric analysis that compares pituitary cells from wild and captive broodstocks during the reproductive annual cycle. The morphology of adenohypophysial cells showed differences following the reproductive cycle and the environment. In general, optical density suggested a higher cellular activity during the previtellogenic (growth hormone) and vitellogenic (somatolactin) stages in both environments. Additionally, the nucleus/cell ratio analysis suggested that growth hormone and somatolactin cells were larger in wild than in captive females in most reproductive stages of the annual cycle. In contrast, prolactin hormone showed no variation throughout the reproductive cycle (in both environments). Morphometrical analyses related to reproduction of S. hilarii in different environmental conditions, suggest that somatolactin and growth hormone play an important role in reproduction in teleost and can be responsible for the regulation of associated processes that indirectly affect reproductive status.
authors.affiliation: Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, Trav. 14, N° 321, 05508-090 São Paulo, SP Brazil.
authors.forename: Renato Massaaki
authors.initials: RM
authors.lastname: Honji
authors.forename: Rafael Henrique
authors.initials: RH
authors.lastname: Nóbrega
authors.forename: Matias
authors.initials: M
authors.lastname: Pandolfi
authors.forename: Akio
authors.initials: A
authors.lastname: Shimizu
authors.forename: Maria Inês
authors.initials: MI
authors.lastname: Borella
authors.forename: Renata Guimarães
authors.initials: RG
authors.lastname: Moreira
keywords: Gonadotropins; Growth hormone; Prolactin hormone; Reproductive dysfunction; Somatolactin hormone