title: Improvement of reticuline productivity from dopamine by using engineered Escherichia coli.
abstract: Benzylisoquinoline alkaloids (BIAs) are pharmaceutically important compounds. We have previously devised a reticuline (BIA) production method from dopamine by using Escherichia coli; however, its productivity was relatively low (33 µM, 11 mg/L). We report here, by fine-tuning the method, higher reticuline productivity of 165 µM (54 mg/L), increasing the conversion efficiency by 8-fold. These results are important for developing an efficient route to fermentative reticuline production.
authors.affiliation: Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University.
authors.forename: Ju-Sung
authors.initials: JS
authors.lastname: Kim
authors.forename: Akira
authors.initials: A
authors.lastname: Nakagawa
authors.forename: Yuya
authors.initials: Y
authors.lastname: Yamazaki
authors.forename: Eitaro
authors.initials: E
authors.lastname: Matsumura
authors.forename: Takashi
authors.initials: T
authors.lastname: Koyanagi
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Minami
authors.forename: Takane
authors.initials: T
authors.lastname: Katayama
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Sato
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Kumagai
mesh_terms: D044182:Benzylisoquinolines / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005285:Fermentation; D060847:Metabolic Engineering / Q000379:methods*
chemical_list: D044182:Benzylisoquinolines; D004298:Dopamine; C003298:reticuline