title: Fluorescent indicator displacement assay of ligands targeting 10 microRNA precursors.
abstract: Fluorescent indicator displacement (FID) assay is a rapid and convenient assay for identifying new ligands that bind to the target molecules. In our previous studies, we have shown that a series of 2,7-diaminoalkoxy xanthone and thioxanthone derivatives can be used as fluorescent indicators for detecting the interaction between RNA and a ligand. The xanthone and thioxanthone fluorochromes showed efficient fluorescence quenching upon binding to target RNA. Subsequent displacement of the bound-fluorochrome with a ligand that binds more strongly to the target RNA led to the recovery of the fluorescence by releasing the fluorochrome from RNA. Here we report a pilot screening of a chemical library that contains 9600 structurally diverse compounds for molecules that bind to a specific miRNA precursor using the FID assay.
authors.affiliation: Department of Regulatory Bioorganic Chemistry, The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki 567-0047, Japan.
authors.forename: Asako
authors.initials: A
authors.lastname: Murata
authors.forename: Yasue
authors.initials: Y
authors.lastname: Harada
authors.forename: Takeo
authors.initials: T
authors.lastname: Fukuzumi
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakatani
mesh_terms: D001483:Base Sequence; D005456:Fluorescent Dyes / Q000737:chemistry*; D008024:Ligands; D035683:MicroRNAs / Q000032:analysis* / Q000378:metabolism; D012322:RNA Precursors / Q000032:analysis / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D044004:Xanthones / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D008024:Ligands; D035683:MicroRNAs; D012322:RNA Precursors; D044004:Xanthones; C009689:xanthone
keywords: FID assay; Fluorescent indicator; Pre-miRNA; Screening; Small molecule