title: Comparative analysis of proteomic profiles between endometrial caruncular and intercaruncular areas in ewes during the peri-implantation period.
abstract: The endometrium of sheep consists of plenty of raised aglandular areas called caruncular (C), and intensely glandular intercaruncular areas (IC). In order to better understand the endometrium involved mechanisms of implantation, we used LC-MS/MS technique to profile the proteome of ovine endometrial C areas and IC areas separately during the peri-implantation period, and then compared the proteomic profiles between these two areas. We successfully detected 1740 and 1813 proteins in C areas and IC areas respectively. By comparing the proteome of these two areas, we found 170 differentially expressed proteins (DEPs) (P < 0.05), functional bioinformatics analysis showed these DEPs were mainly involved in growth and remodeling of endometrial tissue, cell adhesion and protein transport, and so on. Our study, for the first time, provided a proteomic reference for elucidating the differences between C and IC areas, as an integrated function unit respectively, during the peri-implantation period. The results could help us to better understand the implantation in the ewes. In addition, we established a relatively detailed protein database of ovine endometrium, which provide a unique reference for further studies.
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National engineering laboratory for animal breeding, College of Animal Sciences and Technology, China Agricultural University, No.2 Yuanmingyuan Xi Lu, Haidian, Beijing 100193, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National engineering laboratory for animal breeding, College of Animal Sciences and Technology, China Agricultural University, No.2 Yuanmingyuan Xi Lu, Haidian, Beijing 100193, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, China Agricultural University, Beijing 100193, China.
authors.forename: Zhuocheng
authors.initials: Z
authors.lastname: Hou
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National engineering laboratory for animal breeding, College of Animal Sciences and Technology, China Agricultural University, No.2 Yuanmingyuan Xi Lu, Haidian, Beijing 100193, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Miao
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National engineering laboratory for animal breeding, College of Animal Sciences and Technology, China Agricultural University, No.2 Yuanmingyuan Xi Lu, Haidian, Beijing 100193, China.
authors.forename: Haichao
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National engineering laboratory for animal breeding, College of Animal Sciences and Technology, China Agricultural University, No.2 Yuanmingyuan Xi Lu, Haidian, Beijing 100193, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National engineering laboratory for animal breeding, College of Animal Sciences and Technology, China Agricultural University, No.2 Yuanmingyuan Xi Lu, Haidian, Beijing 100193, China.
authors.forename: Min
authors.initials: M
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Sciences and Technology, China Agricultural University, No.2 Yuanmingyuan Xi Lu, Haidian, Beijing 100193, China.
authors.forename: Zhonghong
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National engineering laboratory for animal breeding, College of Animal Sciences and Technology, China Agricultural University, No.2 Yuanmingyuan Xi Lu, Haidian, Beijing 100193, China.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Tian
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National engineering laboratory for animal breeding, College of Animal Sciences and Technology, China Agricultural University, No.2 Yuanmingyuan Xi Lu, Haidian, Beijing 100193, China.
authors.forename: Lei
authors.initials: L
authors.lastname: An