title: DT-MRI measurement of myolaminar structure: accuracy and sensitivity to time post-fixation, b-value and number of directions.
abstract: DT-MRI has been widely used to quantify myocardial fiber and laminar orientations. These structural orientations influence both the spread of excitation and the reorganization of the myocardium during contraction and are altered in disease states. Studies have sought to validate DT-MRI but questions remain about the accuracy of the method and its sensitivity to the time post-fixation and imaging parameters, including b-value, number of diffusion directions and image voxel size. The advent of high-spatial resolution ex vivo MRI and structure tensor (ST) analysis provides a means of direct validation of DT-MRI and assessment of sensitivity to the b-value, the number of diffusion directions and the image voxel size. We find that, with the fixation method we used, structure does not change with time (up to 72 hours). We show that DT-MRI and ST/HR-MRI are markedly similar measures of fiber orientation but DT-MRI and ST are much less similar measures of laminar orientation. DT-MRI performance is not sensitive to the number of directions, with similar structural orientations measured with 6 or 12 directions. Likewise, DT-MRI performance is generally insensitive to b-value, but laminar measurement is moderately more accurate at b = 500 than for higher b-values.
authors.forename: Stephen H
authors.initials: SH
authors.lastname: Gilbert
authors.forename: Bruce H
authors.initials: BH
authors.lastname: Smaill
authors.forename: Richard D
authors.initials: RD
authors.lastname: Walton
authors.forename: Mark L
authors.initials: ML
authors.lastname: Trew
authors.forename: Olivier
authors.initials: O
authors.lastname: Bernus
mesh_terms: D000818:Animals; D038524:Diffusion Magnetic Resonance Imaging; D056324:Diffusion Tensor Imaging; D008297:Male; D009206:Myocardium / Q000473:pathology*; D017208:Rats, Wistar; D013997:Time Factors; D016707:Tissue Fixation