title: RhoD binds the Rab5 effector Rabankyrin-5 and has a role in trafficking of the platelet-derived growth factor receptor.
abstract: RhoD is a member of the classical Rho GTPases and it has essential roles in the regulation of actin dynamics. RhoD localizes to early endosomes and recycling endosomes, which indicates its important role in the regulation of endosome trafficking. Here, we show that RhoD binds to the Rab5 effector Rabankyrin-5, and RhoD and Rabankyrin-5 colocalize to Rab5-positive endosomes, which suggests a role for Rabankyrin-5 in the coordination of RhoD and Rab5 in endosomal trafficking. Interestingly, depletion of RhoD using siRNA techniques interfered with the internalization of the PDGFβ receptor and the subsequent activation of the downstream signaling cascades. Our data suggest that RhoD and Rabankyrin-5 have important roles in coordinating RhoD and Rab activities during internalization and trafficking of activated tyrosine kinase receptors.
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Box 280, Nobels väg 16, SE-171 77, Stockholm, Sweden.
authors.forename: Vishal
authors.initials: V
authors.lastname: Nehru
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Voytyuk
authors.forename: Johan
authors.initials: J
authors.lastname: Lennartsson
authors.forename: Pontus
authors.initials: P
authors.lastname: Aspenström
mesh_terms: D001665:Binding Sites; D011992:Endosomes / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D028044:Phosphate-Binding Proteins; D011485:Protein Binding; D021381:Protein Transport; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000378:metabolism*; D020696:rab5 GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: C118719:ANKFY1 protein, human; D008565:Membrane Proteins; D028044:Phosphate-Binding Proteins; D020797:Receptor, Platelet-Derived Growth Factor beta; D020696:rab5 GTP-Binding Proteins; D020741:rho GTP-Binding Proteins
keywords: Endosome; PDGF receptor; Rab5; Rabankyrin-5; Rho GTPases; RhoD