title: Influence of a silicon (Si14)-based coating substrate for biomaterials on fibroblast growth and human C5a.
abstract: Despite considerable efforts in biomaterial development there is still a lack on substrates for cardiovascular tissue engineering approaches which allow the establishment of a tight a functional endothelial layer on their surface to provide hemocompatibility. The study aimed to test the biocompatibility of a silicon (Si14)-based coating substrate (Supershine Medicare, Permanon) which was designed to resist temperatures from -40°C up to 300°C and which allows the use of established heat-inducing sterilization techniques respectively. By X-ray photoelectron spectroscopy it could be validated that this substrate is able to establish a 40-50 nm thick layer of silica, oxygen and carbon without including any further elements from the substrate on an exemplary selection of materials (silicone, soda-lime-silica glass, stainless steel). Analysis of the LDH-release, the cell activity/proliferation (MTS assay) and the cell phenotype after growing 3T3 cells with extracts of the coated materials did not indicate any signs of cytotoxicity. Additionally by measuring the C5a release after exposure of the coated materials with human serum it could be demonstrated, that the coating had no impact on the activation of the complement system. These results generally suggest the tested substrate as a promising candidate for the coating of materials which are aimed to be used in cardiovascular tissue engineering approaches.
authors.affiliation: Center for Medical Basic Research, Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany.
authors.forename: B
authors.initials: B
authors.lastname: Hiebl
authors.affiliation: Faculty of Veterinary Medicine, Institute of Veterinary Anatomy, Freie Universität Berlin, Berlin, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Hopperdietzel
authors.affiliation: Faculty of Veterinary Medicine, Institute of Veterinary Anatomy, Freie Universität Berlin, Berlin, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Hünigen
authors.affiliation: Institute of Biomaterial Science and Berlin-Brandenburg Centre for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Jung
authors.affiliation: Institute of Materials Research, Magnesium Innovations Center, Helmholtz-Zentrum Geesthacht, Zentrum für Material- und Küstenforschung GmbH, Geesthacht, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Scharnagl
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D048708:Cell Growth Processes / Q000187:drug effects / Q000502:physiology; D015936:Complement C5a / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D010641:Phenotype; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D015936:Complement C5a; D012825:Silicon
keywords: Biomaterial; cardiovascular; coating; silicon; tissue engineering