title: Preparation and in vitro evaluation of mesoporous hydroxyapatite coated β-TCP porous scaffolds.
abstract: A mesoporous hydroxyapatite (HA) coating was prepared on a β-tricalcium phosphate (β-TCP) porous scaffold by a sol-gel dip-coating method using the block copolymer Pluronic F127 (EO106PO70EO106) as the template. For application as a bone graft, in vitro cell response and bone-related protein expression of mesoporous HA coated β-TCP scaffold were investigated, using the non-mesoporous HA coated scaffold as the control group, to evaluate the influence of the mesoporous structure on the biological properties of HA coating. It was found that the increased surface area of the mesoporous HA coating greatly affected the response of MC3T3-E1 osteoblasts and the expression of proteins. An enzyme-linked immunosorbent assay recorded a significantly higher expression of alkaline phosphatase (ALP) and bone sialoprotein (BSP) in the mesoporous group than those in the control group (*p<0.05) after different incubation periods. The introduction of mesopores enhanced the expression of ALP and BSP in the cells grown on the mesoporous HA coatings, on the premise of maintaining the protein expression in a sequence to ensure the correct temporo-spatial expression in osteogenesis. These results indicated that the mesoporous HA coating would provide a good environment for cell growth, suggesting that it could be used as the coating material for the surface modification of the tissue engineering scaffolds.
authors.affiliation: Key Laboratory for Advanced Ceramics and Machining Technology of Ministry of Education, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Ye
authors.forename: Shu
authors.initials: S
authors.lastname: Cai
authors.forename: Guohua
authors.initials: G
authors.lastname: Xu
authors.forename: Ying
authors.initials: Y
authors.lastname: Dou
authors.forename: Hongtao
authors.initials: H
authors.lastname: Hu
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Ye
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry*; D058574:Integrin-Binding Sialoprotein / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D020442:Poloxamer / Q000737:chemistry; D016062:Porosity
chemical_list: D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D058574:Integrin-Binding Sialoprotein; C485817:beta-tricalcium phosphate; D020442:Poloxamer; D017886:Durapatite; D000469:Alkaline Phosphatase
keywords: Cell response; Coatings; Mesoporous hydroxyapatite; Protein expression; β-tricalcium phosphate (β-TCP) scaffold