title: Cloning and sequence analysis of neuropeptide F from the oriental tobacco budworm Helicoverpa assulta (Guenée).
abstract: Neuropeptide F (NPF), the invertebrate homolog of neuropeptide Y (NPY) in vertebrates, shares similarity of structure and function with NPY. However, a few NPYs were also found in some insect species. In this paper, two neuropeptide genes encoding a NPF and a NPY were cloned from a tobacco budworm Helicoverpa assulta cDNA library. The npf1 gene further produces two splicing variants of rnRNAs, i.e. npf1a (lacks the 120 bp segment) and npf1b (includes a 120 bp segment). These two splicing variants form two mature peptides, NPF1a and NPF1b by modification of transcripts. NPF and NPY co-exist in H. assulta.
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, China.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Liu
authors.forename: Yifan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zijing
authors.initials: Z
authors.lastname: Zhou
authors.forename: Zhangwu
authors.initials: Z
authors.lastname: Zhao
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D009478:Neuropeptide Y / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009479:Neuropeptides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D009478:Neuropeptide Y; D009479:Neuropeptides; D012333:RNA, Messenger; C076101:neuropeptide F
keywords: Helicoverpa assulta; NPY-like peptides; Neuropeptide F; Neuropeptide Y