title: Simultaneous telemetric monitoring of brain glucose and lactate and motion in freely moving rats.
abstract: A new telemetry system for simultaneous detection of extracellular brain glucose and lactate and motion is presented. The device consists of dual-channel, single-supply miniature potentiostat-I/V converter, a microcontroller unit, a signal transmitter, and a miniaturized microvibration sensor. Although based on simple and inexpensive components, the biotelemetry device has been used for accurate transduction of the anodic oxidation currents generated on the surface of implanted glucose and lactate biosensors and animal microvibrations. The device was characterized and validated in vitro before in vivo experiments. The biosensors were implanted in the striatum of freely moving animals and the biotelemetric device was fixed to the animal's head. Physiological and pharmacological stimulations were given in order to induce striatal neural activation and to modify the motor behavior in awake, untethered animals.
authors.affiliation: Department of Clinical and Experimental Medicine, Medical School, University of Sassari , Viale S. Pietro 43/b, 07100 Sassari, Italy.
authors.forename: Gaia
authors.initials: G
authors.lastname: Rocchitta
authors.forename: Ottavio
authors.initials: O
authors.lastname: Secchi
authors.forename: Maria Domenica
authors.initials: MD
authors.lastname: Alvau
authors.forename: Donatella
authors.initials: D
authors.lastname: Farina
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Bazzu
authors.forename: Giammario
authors.initials: G
authors.lastname: Calia
authors.forename: Rossana
authors.initials: R
authors.lastname: Migheli
authors.forename: Maria Speranza
authors.initials: MS
authors.lastname: Desole
authors.forename: Robert D
authors.initials: RD
authors.lastname: O'Neill
authors.forename: Pier A
authors.initials: PA
authors.lastname: Serra
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D001921:Brain / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013686:Telemetry
chemical_list: D007773:Lactates; D005947:Glucose