title: Molecular diagnosis of Actinomadura madurae infection by 16S rRNA deep sequencing.
abstract: Next-generation DNA sequencing can be used to catalog individual organisms within complex, polymicrobial specimens. Here, we utilized deep sequencing of 16S rRNA to implicate Actinomadura madurae as the cause of mycetoma in a diabetic patient when culture and conventional molecular methods were overwhelmed by overgrowth of other organisms.
authors.affiliation: Departments of Laboratory Medicine.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Salipante
authors.forename: Dhruba J
authors.initials: DJ
authors.lastname: Sengupta
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Hoogestraat
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Cummings
authors.forename: Bronwyn H
authors.initials: BH
authors.lastname: Bryant
authors.forename: Catherine
authors.initials: C
authors.lastname: Natividad
authors.forename: Stephanie
authors.initials: S
authors.lastname: Thielges
authors.forename: Peter W
authors.initials: PW
authors.lastname: Monsaas
authors.forename: Mimosa
authors.initials: M
authors.lastname: Chau
authors.forename: Lindley A
authors.initials: LA
authors.lastname: Barbee
authors.forename: Christopher
authors.initials: C
authors.lastname: Rosenthal
authors.forename: Brad T
authors.initials: BT
authors.lastname: Cookson
authors.forename: Noah G
authors.initials: NG
authors.lastname: Hoffman
mesh_terms: D000192:Actinomycetales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D048909:Diabetes Complications; D005260:Female; D005528:Foot / Q000473:pathology; D059014:High-Throughput Nucleotide Sequencing; D006651:Histocytochemistry; D006801:Humans; D008853:Microscopy; D008875:Middle Aged; D008969:Molecular Sequence Data; D008271:Mycetoma / Q000175:diagnosis* / Q000382:microbiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S