title: Identification and function of two myeloid differentiation factor 88 variants in triangle-shell pearl mussel (Hyriopsis cumingii).
abstract: Myeloid differentiation factor 88 (MyD88) is a universal and essential adapter protein that participates in the activation of the Toll-like receptor (TLR)/interleukin-1 receptor-mediated signaling pathway. In this study, two MyD88 genes (HcMyD88-1 and HcMyD88-2) were identified from triangle-shell pearl mussel (Hyriopsis cumingii). Both HcMyD88-1 and HcMyD88-2 proteins were determined to have a death domain at the N-terminal and a TIR domain at the C-terminal. Both HcMyD88-1 and HcMyD88-2 genes were mainly expressed in the hepatopancreas of healthy mussels. HcMyD88-1 and HcMyD88-2 slightly responded to Gram-negative bacterial challenge. Upon bacterial challenge with Gram-positive Staphyloccocus aureus, HcMyD88-1 and HcMyD88-2 transcription levels remarkably increased at 2 and 6h, respectively. Overexpression of HcMyD88-1 and HcMyD88-2 proteins in Drosophila Schneider 2 cells led to the activation of antimicrobial peptide genes. These results indicated that HcMyD88-2 had higher activity than HcMyD88-1 during the activation of attacin A, drosomycin, and metchnikowin genes, suggesting that HcMyD88 genes may play a role in antibacterial innate immune defense.
authors.affiliation: Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, China. Electronic address: renqian0402@126.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ren
authors.forename: Yi-Hong
authors.initials: YH
authors.lastname: Chen
authors.forename: Zheng-Feng
authors.initials: ZF
authors.lastname: Ding
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Yan-Ru
authors.initials: YR
authors.lastname: Shi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000276:immunology / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004330:Drosophila / Q000276:immunology; D029721:Drosophila Proteins / Q000276:immunology / Q000378:metabolism; D014644:Genetic Variation; D008969:Molecular Sequence Data; D053594:Myeloid Differentiation Factor 88 / Q000145:classification / Q000235:genetics / Q000276:immunology*; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D013203:Staphylococcal Infections / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology; D049876:Unionidae / Q000235:genetics / Q000276:immunology* / Q000382:microbiology
chemical_list: D023181:Antimicrobial Cationic Peptides; C488990:AttA protein, Drosophila; D029721:Drosophila Proteins; D053594:Myeloid Differentiation Factor 88; D020033:Protein Isoforms; C091227:DRS protein, Drosophila; C096696:Mtk protein, Drosophila
keywords: Antibacterial peptides; Hyriopsis cumingii; MyD88; Overexpression