title: Bacteriological and pathological studies of egg peritonitis in commercial layer chicken in Namakkal area.
abstract: OBJECTIVE
  To detect the various bacteriological agents and pathological changes in commercial layer chicken affected with egg yolk peritonitis in Namakkal region of India.
  
  
  METHODS
  A total of 6 572 layer chicken from 85 commercial farms were subjected for the study, out of which 1 715 showed various types of oviduct abnormalities. Among the 1 715, 264 birds from six farms were identified as egg peritonitis on the basis of postmortem examination. Trachea, lung, heart blood, liver, peritoneal exudate, oviduct (infundibulum, magnum, uterus) and cloacal swabs were collected from the 264 birds with egg peritonitis lesion for screening of bacterial agents. Signalment, clinical signs and pathological changes were recorded in the affected flocks.
  
  
  RESULTS
  The results of the present investigation indicated that the E. coli associated egg peritonitis was responsible for 15.39% of the reproductive tract abnormalities in commercial layers between 21 and 80 week of age. In the affected flocks egg production drop and mortality varied from 3% to 20% and 0.5% to 7.0% respectively. It was noticed during peak egg production (21 to 60 week) and southwest monsoon season (58%). Statistical analysis of age, season and egg production by Chi square test of independence revealed highly significant difference. E. coli was isolated as a pure culture and concurrent with other bacterial agents in 226 and 38 birds respectively. Among the fifteen E. coli serotypes identified serotype O166, O64 and O111 were predominant. Necropsy examination of affected birds revealed the presence of amorphous or insipissiated yolk material in the abdominal cavity with inflammatory changes in the ovary, oviduct and intestine. Microscopically the oviduct surface epithelium showed degeneration and desquamation, moderate to marked infiltration of inflammatory cells especially heterophils and lymphocytes in various regions and lumen contained serofibrinous exudate, inflammatory and desquamated epithelial cells with bacterial microcolonies. Ovarian follicles revealed hyperemia, degeneration of granulosa cells and infiltration of inflammatory cells. Intestine showed degenerative, necrotic and inflammatory lesion.
  
  
  CONCLUSIONS
  The findings of this study showed that the egg peritonitis might be caused by either the translocation of intestinal E. coli into the peritoneal cavity or by the movement of cloacal E. coli into the oviduct followed by ascension of these bacteria up the oviduct, through the infundibulum, and into the peritoneal cavity. To control the egg peritonitis faecal contamination with E. coli should be minimized.
authors.affiliation: Department of Veterinary Pathology, Veterinary College and Research Institute, Namakkal, Tamil Nadu State, India. Electronic address: srinipat2004@yahoo.com.
authors.forename: Palani
authors.initials: P
authors.lastname: Srinivasan
authors.forename: Gurusamypalayam Amirthalingam
authors.initials: GA
authors.lastname: Balasubramaniam
authors.forename: Thippichettipalayam Ramasamy Gopala Krishna
authors.initials: TR
authors.lastname: Murthy
authors.forename: Perumal
authors.initials: P
authors.lastname: Balachandran
mesh_terms: D000825:Animal Structures / Q000382:microbiology / Q000473:pathology; D000818:Animals; D002645:Chickens; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D007194:India; D010538:Peritonitis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D011201:Poultry Diseases / Q000382:microbiology* / Q000473:pathology*; D012703:Serotyping; D016019:Survival Analysis
keywords: E. coli; Egg peritonitis; Layer chicken; Pathology; Prevalence