title: Liquid chromatographic determinations of meta-chlorobenzoic acid in bupropion hydrochloride.
abstract: A simple, sensitive method for the determination of meta-chlorobenzoic acid in bupropion hydrochloride is described. Chromatographic separation of m-chlorobenzoic acid is achieved using a mobile phase consisting of n-hexane and ethanol (1000:50, v/v) at a flow rate of 1.0 ml/min on a Chiralpak ADH (250 × 4.6 mm). Absorbance is monitored at 235 nm. The method is linear for m-chlorobenzoic acid over concentration range of LOQ, 5.0 μg/ml to 15.0 μg/ml for m-chlorobenzoic acid with correlation coefficient greater than 0.99. This method is more selective and accurate than United States Pharmacopoeia method for the determination of m-chlorobenzoic acid in bupropion hydrochloride.
authors.affiliation: Department of Analytical Research Lab, Glenmark Generics Ltd, Glenmark Research Centre, Plot A - 607, TTC Industrial Area, MIDC, Mahape, Navi Mumbai, Maharashtra, India.
authors.forename: Sukumar
authors.initials: S
authors.lastname: Sinha
authors.forename: Vikas
authors.initials: V
authors.lastname: Parashar
authors.forename: K Swamy
authors.initials: KS
authors.lastname: Reddy
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Khan
authors.forename: S K Manirul
authors.initials: SK
authors.lastname: Haque
keywords: 1-(3-Chlorophenyl)-1,2-propanedione; HPLC method; bupropion Hcl; meta-chlorobenzoic acid; method validation