title: A dual sensor for real-time monitoring of glucose and oxygen.
abstract: A dual glucose and oxygen sensor in a polymer format was developed. The dual sensor composed of a blue emitter as the glucose probe, a red emitter as an oxygen probe, and a yellow emitter as a built-in reference probe which does not respond to either glucose or oxygen. All the three probes were chemically immobilized in a polyacrylamide-based matrix. Therefore, the dual sensor possesses three well separated emission colors and ratiometric approach is applicable for analysis of the glucose and oxygen concentration at biological conditions. The sensor was applied for real-time monitoring of glucose and oxygen consumption of bacterial cells, Escherichia coli (E. coli) and Bacillus subtilis (B. subtilis), and mammalian cells of mouse macrophage J774 and human cervical cancer HeLa cell lines. On the other hand, in order to achieve satisfactory sensing performance for glucose, compositions of the matrices among poly(2-hydroxyethyl methacrylate), polyacrylamide, and poly(6-aminohexyl methacrylamide) which is a linker polymer for grafting the glucose probe, were optimized.
authors.affiliation: Center for Biosignatures Discovery Automation, Biodesign Institute, Arizona State University, Tempe 85287-6501, USA.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Zhang
authors.forename: Fengyu
authors.initials: F
authors.lastname: Su
authors.forename: Sean
authors.initials: S
authors.lastname: Buizer
authors.forename: Hongguang
authors.initials: H
authors.lastname: Lu
authors.forename: Weimin
authors.initials: W
authors.lastname: Gao
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Tian
authors.forename: Deirdre
authors.initials: D
authors.lastname: Meldrum
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D001412:Bacillus subtilis / Q000378:metabolism; D015374:Biosensing Techniques; D002460:Cell Line; D004926:Escherichia coli / Q000378:metabolism; D005947:Glucose / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D015394:Molecular Structure; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism*; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D005947:Glucose; D010100:Oxygen; D000099166:Polyacrylamides
keywords: Dual sensor; Glucose sensor; Oxygen sensor; Ratiometric; Sensor