title: Live visualization of chromatin dynamics with fluorescent TALEs.
abstract: The spatiotemporal organization of genomes in the nucleus is an emerging key player to regulate genome function. Live imaging of nuclear organization dynamics would be a breakthrough toward uncovering the functional relevance and mechanisms regulating genome architecture. Here, we used transcription activator-like effector (TALE) technology to visualize endogenous repetitive genomic sequences. We established TALE-mediated genome visualization (TGV) to label genomic sequences and follow nuclear positioning and chromatin dynamics in cultured mouse cells and in the living organism. TGV is highly specific, thus allowing differential labeling of parental chromosomes by distinguishing between single-nucleotide polymorphisms (SNPs). Our findings provide a framework to address the function of genome architecture through visualization of nuclear dynamics in vivo.
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Université de Strasbourg, Illkirch, France.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Miyanari
authors.forename: Céline
authors.initials: C
authors.lastname: Ziegler-Birling
authors.forename: Maria-Elena
authors.initials: ME
authors.lastname: Torres-Padilla
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry* / Q000378:metabolism*; D002478:Cells, Cultured; D002843:Chromatin / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D002843:Chromatin