title: Tyrosine phosphorylation is essential for DSCAML1 to promote dendrite arborization of mouse cortical neurons.
abstract: Dendritic self-avoidance is critical for appropriate dendrite arborization. We herein examined the role of Down syndrome cell adhesion molecule like-1 (DSCAML1) in regulating dendritic self-avoidance and that of tyrosine phosphorylation in mediating the effects of DSCAML1. Knocking down DSCAML1 in newborn mouse cortical neurons compromised dendritic self-avoidance as evidenced by dendritic fasciculation and increased dendritic self-crossing. Introduction of a DSCAML1(Y1808F) mutant into the DSCAML1-knocked down neurons failed to reverse the abnormal dendritic arborization. These results suggest that DSCAML1 promotes dendritic self-avoidance in cortical neurons, and that phosphorylation at Y1808 is essential in mediating the effects of DSCAML1.
authors.affiliation: Department of Orthopedics, Shengjing Hospital, China Medical University, Shenyang, China. Electronic address: cuisq@sj-hospital.org.
authors.forename: Shaoqian
authors.initials: S
authors.lastname: Cui
authors.forename: Limin
authors.initials: L
authors.lastname: Lao
authors.forename: Jingzhu
authors.initials: J
authors.lastname: Duan
authors.forename: Guoxin
authors.initials: G
authors.lastname: Jin
authors.forename: Xu
authors.initials: X
authors.lastname: Hou
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism / Q000648:ultrastructure*; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D055785:Gene Knockdown Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure*; D010766:Phosphorylation; D061251:Primary Cell Culture; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D015815:Cell Adhesion Molecules; C486620:DSCAML1 protein, mouse; D014443:Tyrosine
keywords: DIV; DSCAM; DSCAML1; Dendrite arborization; Down syndrome cell adhesion molecule; Down syndrome cell adhesion molecule like-1; PAK1; RNA interference; Self-avoidance; Tyrosine phosphorylation; day in vitro; p21 activated kinase