title: High throughput colorimetric assay for rapid urease activity quantification.
abstract: A novel high throughput colorimetric urease activity assay was compared to the Nessler method. The new method employs phenol red to determine the urease activity. This method reduces significantly sample processing time and allows real-time investigations. This method is rapid, sensitive, easy, cost-effective, and does not use any toxic chemical reagents.
authors.affiliation: Department of Civil and Environmental Engineering, University of Houston, Houston, TX, 77004, USA.
authors.forename: Tugba
authors.initials: T
authors.lastname: Onal Okyay
authors.forename: Debora
authors.initials: D
authors.lastname: Frigi Rodrigues
mesh_terms: D003124:Colorimetry / Q000379:methods*; D003365:Costs and Cost Analysis; D010637:Phenolsulfonphthalein / Q000378:metabolism; D012680:Sensitivity and Specificity; D013997:Time Factors; D014510:Urease / Q000032:analysis*
chemical_list: D014510:Urease; D010637:Phenolsulfonphthalein
keywords: Colorimetric assay; High throughput; Urease activity