title: Development of a tightly regulated and highly responsive copper-inducible gene expression system and its application to control of flowering time.
abstract: A newly developed copper-inducible gene expression system overcame the mixed results reported earlier, worked well both in cultured cells and a whole plant, and enabled to control flowering timing. Copper is one of the essential microelements and is readily taken up by plants. However, to date, it has rarely been used to control the expression of genes of interest, probably due to the inefficiency of the gene expression systems. In this study, we successfully developed a copper-inducible gene expression system that is based on the regulation of the yeast metallothionein gene. This system can be applied in the field and regulated at approximately one-hundredth of the rate used for registered copper-based fungicides. In the presence of copper, a translational fusion of the ACE1 transcription factor with the VP16 activation domain (VP16AD) of herpes simplex virus strongly activated transcription of the GFP gene in transgenic Arabidopsis. Interestingly, insertion of the To71 sequence, a 5'-untranslated region of the 130k/180k gene of tomato mosaic virus, upstream of the GFP gene reduced the basal expression of GFP in the absence of copper to almost negligible levels, even in soil-grown plants that were supplemented with ordinary liquid nutrients. Exposure of plants to 100 μM copper resulted in an over 1,000-fold induction ratio at the transcriptional level of GFP. This induction was copper-specific and dose-dependent with rapid and reversible responses. Using this expression system, we also succeeded in regulating floral transition by copper treatment. These results indicate that our newly developed copper-inducible system can accelerate gene functional analysis in model plants and can be used to generate novel agronomic traits in crop species.
authors.affiliation: Biology Group, Discovery, Health & Crop Sciences Research Laboratory, Sumitomo Chemical Co., Ltd., 4-2-1, Takatsukasa, Takarazuka, Hyogo, 665-8555, Japan, saijout@sc.sumitomo-chem.co.jp.
authors.forename: Takanori
authors.initials: T
authors.lastname: Saijo
authors.forename: Akitsu
authors.initials: A
authors.lastname: Nagasawa
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D003300:Copper / Q000494:pharmacology*; D035264:Flowers / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D049452:Green Fluorescent Proteins; D003300:Copper