title: Comparative analysis of ESBL-positive Escherichia coli isolates from animals and humans from the UK, The Netherlands and Germany.
abstract: The putative virulence and antimicrobial resistance gene contents of extended spectrum β-lactamase (ESBL)-positive E. coli (n=629) isolated between 2005 and 2009 from humans, animals and animal food products in Germany, The Netherlands and the UK were compared using a microarray approach to test the suitability of this approach with regard to determining their similarities. A selection of isolates (n=313) were also analysed by multilocus sequence typing (MLST). Isolates harbouring bla(CTX-M-group-1) dominated (66%, n=418) and originated from both animals and cases of human infections in all three countries; 23% (n=144) of all isolates contained both bla(CTX-M-group-1) and bla(OXA-1-like) genes, predominantly from humans (n=127) and UK cattle (n=15). The antimicrobial resistance and virulence gene profiles of this collection of isolates were highly diverse. A substantial number of human isolates (32%, n=87) did not share more than 40% similarity (based on the Jaccard coefficient) with animal isolates. A further 43% of human isolates from the three countries (n=117) were at least 40% similar to each other and to five isolates from UK cattle and one each from Dutch chicken meat and a German dog; the members of this group usually harboured genes such as mph(A), mrx, aac(6')-Ib, catB3, bla(OXA-1-like) and bla(CTX-M-group-1). forty-four per cent of the MLST-typed isolates in this group belonged to ST131 (n=18) and 22% to ST405 (n=9), all from humans. Among animal isolates subjected to MLST (n=258), only 1.2% (n=3) were more than 70% similar to human isolates in gene profiles and shared the same MLST clonal complex with the corresponding human isolates. The results suggest that minimising human-to-human transmission is essential to control the spread of ESBL-positive E. coli in humans.
authors.affiliation: Animal Health and Veterinary Laboratories Agency (AHVLA, Weybridge), Addlestone, United Kingdom.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Wu
authors.forename: Michaela J
authors.initials: MJ
authors.lastname: Day
authors.forename: Muriel T
authors.initials: MT
authors.lastname: Mafura
authors.forename: Javier
authors.initials: J
authors.lastname: Nunez-Garcia
authors.forename: Jackie J
authors.initials: JJ
authors.lastname: Fenner
authors.forename: Meenaxi
authors.initials: M
authors.lastname: Sharma
authors.forename: Alieda
authors.initials: A
authors.lastname: van Essen-Zandbergen
authors.forename: Irene
authors.initials: I
authors.lastname: Rodríguez
authors.forename: Cindy
authors.initials: C
authors.lastname: Dierikx
authors.forename: Kristina
authors.initials: K
authors.lastname: Kadlec
authors.forename: Anne-Kathrin
authors.initials: AK
authors.lastname: Schink
authors.forename: Marie
authors.initials: M
authors.lastname: Chattaway
authors.forename: John
authors.initials: J
authors.lastname: Wain
authors.forename: Reiner
authors.initials: R
authors.lastname: Helmuth
authors.forename: Beatriz
authors.initials: B
authors.lastname: Guerra
authors.forename: Stefan
authors.initials: S
authors.lastname: Schwarz
authors.forename: John
authors.initials: J
authors.lastname: Threlfall
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Woodward
authors.forename: Neil
authors.initials: N
authors.lastname: Woodford
authors.forename: Nick
authors.initials: N
authors.lastname: Coldham
authors.forename: Dik
authors.initials: D
authors.lastname: Mevius
mesh_terms: D000821:Animal Feed / Q000382:microbiology; D000818:Animals; D002417:Cattle / Q000382:microbiology*; D002645:Chickens / Q000382:microbiology*; D004285:Dogs / Q000382:microbiology*; D004351:Drug Resistance / Q000235:genetics*; D004926:Escherichia coli / Q000302:isolation & purification* / Q000378:metabolism / Q000472:pathogenicity*; D005858:Germany; D006801:Humans; D046228:Microarray Analysis; D058885:Multilocus Sequence Typing; D009426:Netherlands; D013045:Species Specificity; D006113:United Kingdom; D014774:Virulence; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001618:beta-Lactamases