title: TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells.
abstract: OBJECTIVE
  Oxygen deprivation leading to hypoxia represents a common feature of advanced solid tumors, able to control several aspects of tumor progression. Indeed, ability to respond to changes in oxygen partial pressure represents a hallmark of malignant cells. Aim of this study is to disclose new pathway of hypoxia-induced tumor cell invasion.
  
  
  METHODS
  Hs294T human melanoma cells were grown in a gas mixture containing 0.3% O2 and used to evaluate invasion on Matrigel-coated polycarbonate filters mounted in Boyden's chambers, MMP release and expression of inflammatory receptors and their ligands.
  
  
  RESULTS
  We demonstrate that hypoxia promotes the expression of TNFα receptor 1 (TNFαR1) able to drive a higher ability to penetrate Matrigel-coated filters of Hs294T human melanoma cells, an effect does not mediated by hypoxia-inducible factor (HIF)-1α.
  
  
  CONCLUSIONS
  Expression of inflammatory cytokine receptors in hypoxic human melanoma cells might provide a new target for improving strategies against local and distant tumor cell diffusion.
authors.affiliation: Departament of Experimental and Clinical Biomedical Sciences, Section of Experimental Pathology and Oncology, University of Florence, Istituto Toscano Tumori, Florence, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Marconi
authors.forename: S
authors.initials: S
authors.lastname: Peppicelli
authors.forename: F
authors.initials: F
authors.lastname: Bianchini
authors.forename: L
authors.initials: L
authors.lastname: Calorini
mesh_terms: D015153:Blotting, Western; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology*; D009361:Neoplasm Invasiveness / Q000473:pathology*; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D018836:Inflammation Mediators; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha