title: [In vitro cell response to chemotherapeutic agents, to personalize ovarian cancer treatment: report of two cases].
abstract: Our laboratory has implemented an in vitro assay to estimate the response to chemotherapy in ovarian cancer cells pertaining to individual patients. In two selected patients, we determined the correlation between an in vitro assay of cells from suspected ovarian cancer ascites, with the clinical chemotherapy response. Cancer cells isolated from peritoneal fluid with suspected ovarian cancer were tested for cytotoxicity with corresponding chemotherapy regimens. Circulating Cal25 levels and attending physician consultation determined clinical course and response to chemotherapy. The in vitro assay result correlated with Cal25 levels, progression free survival and attending physician evaluation. The assay predicted correctly the failure of two successive chemotherapy regimes in the first patient, while predicting a favorable clinical response in the second subject.
authors.affiliation: Departamento de Hematología y Oncología, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: C
authors.initials: C
authors.lastname: Carolina Ibáñez
authors.forename: S
authors.initials: S
authors.lastname: Marcelo Garrido
authors.forename: Lidia
authors.initials: L
authors.lastname: Medina
authors.forename: Sumie
authors.initials: S
authors.lastname: Kato
authors.forename: María Loreto
authors.initials: ML
authors.lastname: Bravo
authors.forename: Pamela
authors.initials: P
authors.lastname: González
authors.forename: Barbara
authors.initials: B
authors.lastname: Oliva
authors.forename: Javier
authors.initials: J
authors.lastname: Pizarro
authors.forename: Eva
authors.initials: E
authors.lastname: Bustamante
authors.forename: Jorge
authors.initials: J
authors.lastname: Brañes
authors.forename: Mauricio
authors.initials: M
authors.lastname: Cuello
authors.forename: Gareth I
authors.initials: GI
authors.lastname: Owen
mesh_terms: D000328:Adult; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000008:administration & dosage* / Q000009:adverse effects; D014408:Biomarkers, Tumor / Q000032:analysis; D018394:CA-125 Antigen / Q000032:analysis; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000097:blood / Q000188:drug therapy*; D057285:Precision Medicine; D012074:Remission Induction; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D018394:CA-125 Antigen