title: Antitumor activity of an enzyme prodrug therapy targeted to the breast tumor vasculature.
abstract: The L-methioninase-annexin V/selenomethionine enzyme prodrug system, designed to target the tumor vasculature and release the methylselenol anticancer drug in the tumor, was tested in mice with implanted MBA-MB-231 breast tumors. This therapy was able to cause a reduction in the size of the tumors during the treatment period. It was shown that L-methioninase-annexin V was uniformly bound at the blood vessel surface in the tumor and also that there was a substantial cutoff of blood flowing through the treated tumor, consistent with the therapy's design. This new approach for enzyme prodrug therapy of breast cancer appears promising.
authors.affiliation: 1Bioengineering Center and the School of Chemical, Biological and Materials Engineering, University of Oklahoma , Norman, Oklahoma , USA.
authors.forename: Brent D
authors.initials: BD
authors.lastname: Van Rite
authors.forename: John J
authors.initials: JJ
authors.lastname: Krais
authors.forename: Mohamad
authors.initials: M
authors.lastname: Cherry
authors.forename: Vassilios I
authors.initials: VI
authors.lastname: Sikavitsas
authors.forename: Carla
authors.initials: C
authors.lastname: Kurkjian
authors.forename: Roger G
authors.initials: RG
authors.lastname: Harrison
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001943:Breast Neoplasms / Q000098:blood supply / Q000188:drug therapy; D013437:Carbon-Sulfur Lyases / Q000378:metabolism*; D045744:Cell Line, Tumor; D057487:Enzyme Therapy; D005260:Female; D006801:Humans; D015674:Mammary Neoplasms, Animal / Q000098:blood supply / Q000188:drug therapy*; D000432:Methanol / Q000031:analogs & derivatives* / Q000627:therapeutic use; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D016566:Organoselenium Compounds / Q000627:therapeutic use*; D011355:Prodrugs / Q000378:metabolism / Q000627:therapeutic use; D012645:Selenomethionine / Q000378:metabolism*
chemical_list: D017304:Annexin A5; D000970:Antineoplastic Agents; D016566:Organoselenium Compounds; D011355:Prodrugs; C019003:methaneselenol; D012645:Selenomethionine; D013437:Carbon-Sulfur Lyases; C021558:L-methionine gamma-lyase; D000432:Methanol