title: Developmental cis-regulatory analysis of the cyclin D gene in the sea urchin Strongylocentrotus purpuratus.
abstract: Cyclin D genes regulate the cell cycle, growth and differentiation in response to intercellular signaling. While the promoters of vertebrate cyclin D genes have been analyzed, the cis-regulatory sequences across an entire cyclin D locus have not. Doing so would increase understanding of how cyclin D genes respond to the regulatory states established by developmental gene regulatory networks, linking cell cycle and growth control to the ontogenetic program. Therefore, we conducted a cis-regulatory analysis on the cyclin D gene, SpcycD, of the sea urchin, Strongylocentrotus purpuratus, during embryogenesis, identifying upstream and intronic sequences, located within six defined regions bearing one or more cis-regulatory modules each.
authors.affiliation: Mount Desert Island Biological Laboratory, PO Box 35, Salisbury Cove, ME 04672, USA; The Graduate School of Biomedical Science and Engineering, The University of Maine, Orono, ME 04469, USA. Electronic address: cmccarty@mdibl.org.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: McCarty
authors.forename: James A
authors.initials: JA
authors.lastname: Coffman
mesh_terms: D000818:Animals; D056741:Cyclin D / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks; D011401:Promoter Regions, Genetic; D047270:Strongylocentrotus purpuratus / Q000196:embryology* / Q000235:genetics*
chemical_list: D056741:Cyclin D
keywords: 5′ UTR; 5′ untranslated region; CRM; Cyclin D; Development; GRN; Sea urchin embryo; Strongylocentrotus purpuratus; cis-Regulatory analysis; cis-regulatory module; gene regulatory network; hours post-fertilization; hpf