title: Enumeration of labile hydrogens in natural organic matter by use of hydrogen/deuterium exchange Fourier transform ion cyclotron resonance mass spectrometry.
abstract: A method to enumerate labile hydrogens in all constituents of molecular ensemble of natural organic matter (NOM) based on our previously developed simple hydrogen/deuterium (H/D) exchange (electrospray ionization (ESI) ion source (Kostyukevich et al. Anal. Chem. 2013, 85, 5330) and ultra-high-resolution Fourier transform ion cyclotron resonance mass spectrometry is presented. The method was applied for analysis of Suwannee River fulvic acid (SRFA), which is an International Humic Substances Society standard, as well as Siberian crude oil; and lignosulfonate. We found that SRFA and lignosulfonate molecules contain 2-5 labile hydrogens, and their number increases with the number of oxygens in the molecule. Also, we observed that compounds of Siberian crude oil ionizing in positive-ESI mode do not have labile hydrogens, while compounds ionizing in negative-ESI mode have one labile hydrogen that detaches during ESI ionization.
authors.affiliation: Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences , Leninskij pr. 38 k.2, 119334 Moscow, Russia.
authors.forename: Yury
authors.initials: Y
authors.lastname: Kostyukevich
authors.forename: Alexey
authors.initials: A
authors.lastname: Kononikhin
authors.forename: Igor
authors.initials: I
authors.lastname: Popov
authors.forename: Oleg
authors.initials: O
authors.lastname: Kharybin
authors.forename: Irina
authors.initials: I
authors.lastname: Perminova
authors.forename: Andrey
authors.initials: A
authors.lastname: Konstantinov
authors.forename: Eugene
authors.initials: E
authors.lastname: Nikolaev
mesh_terms: D001578:Benzopyrans / Q000032:analysis; D017357:Cyclotrons; D003903:Deuterium / Q000737:chemistry*; D005583:Fourier Analysis; D005618:Fresh Water / Q000032:analysis*; D006812:Humic Substances / Q000032:analysis*; D006859:Hydrogen / Q000737:chemistry*; D008031:Lignin / Q000031:analogs & derivatives / Q000032:analysis; D010578:Petroleum / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D001578:Benzopyrans; D006812:Humic Substances; D010578:Petroleum; D006859:Hydrogen; C001545:lignosulfuric acid; D008031:Lignin; D003903:Deuterium; C005023:fulvic acid