title: Nonhuman transferrin receptor 1 is an efficient cell entry receptor for Ocozocoautla de Espinosa virus.
abstract: Ocozocoautla de Espinosa virus (OCEV) is a novel, uncultured arenavirus. We found that the OCEV glycoprotein mediates entry into grivet and bat cells through transferrin receptor 1 (TfR1) binding but that OCEV glycoprotein precursor (GPC)-pseudotyped retroviruses poorly entered 53 human cancer cell lines. Interestingly, OCEV and Tacaribe virus could use bat, but not human, TfR1. Replacing three human TfR1 amino acids with their bat ortholog counterparts transformed human TfR1 into an efficient OCEV and Tacaribe virus receptor.
authors.affiliation: Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, USA.
authors.forename: Yíngyún
authors.initials: Y
authors.lastname: Caì
authors.forename: Shuiqìng
authors.initials: S
authors.lastname: Yú
authors.forename: Steven
authors.initials: S
authors.lastname: Mazur
authors.forename: Lián
authors.initials: L
authors.lastname: Dong
authors.forename: Krisztina
authors.initials: K
authors.lastname: Janosko
authors.forename: Téngfei
authors.initials: T
authors.lastname: Zhang
authors.forename: Marcel A
authors.initials: MA
authors.lastname: Müller
authors.forename: Lisa E
authors.initials: LE
authors.lastname: Hensley
authors.forename: Sina
authors.initials: S
authors.lastname: Bavari
authors.forename: Peter B
authors.initials: PB
authors.lastname: Jahrling
authors.forename: Sheli R
authors.initials: SR
authors.lastname: Radoshitzky
authors.forename: Jens H
authors.initials: JH
authors.lastname: Kuhn
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D001117:Arenaviridae Infections / Q000235:genetics / Q000378:metabolism* / Q000662:veterinary* / Q000821:virology; D006477:Arenaviruses, New World / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D002685:Chiroptera / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D002522:Chlorocebus aethiops / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D006801:Humans; D008969:Molecular Sequence Data; D011990:Receptors, Transferrin / Q000235:genetics / Q000378:metabolism*; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism; D053586:Virus Internalization
chemical_list: D015703:Antigens, CD; C068126:CD71 antigen; D011990:Receptors, Transferrin; D011991:Receptors, Virus; D014759:Viral Envelope Proteins