title: Effect of miR27a on proliferation and invasion in colonic cancer cells.
abstract: The aim of this study was to detect the expression of miR196a, miR146a, miR27a and miR200a in patients with colon cancer, and investigate the effect of miR27a expression on proliferation and invasion in colonic cancer cells. RT-PCR was employed to detect the expression levels in colon cancers. Then, colon cancer cells were cultured and transfected with 100 nM of miR27a mimics (80 nmol/L) or 80 nM miR27a inhibitors (80 nmol/L) in 24-well plates. Proliferation and invasion of colonic cancer cells were then determined by CCK-8 and Transwell assays, respectively. Our data showed miR27a to be high-expressed in patients with colon cancer. In addition, proliferation and invasion in the miR27a mimic group were significantly higher than in the control group and negative group (P<0.05), while, proliferation and invasion in the miR27a inhibitor group were obviously lowered (P<0.05). In conclusion, high expression of miR27a may play an important role in enhancing proliferation and invasion of colon cancer cells.
authors.affiliation: Department of General Surgery, Henan Cancer Hospital, Zhengzhou, Henan, China E-mail : yanggaocn@126.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.forename: Bao-Dong
authors.initials: BD
authors.lastname: Li
authors.forename: Yong-Gang
authors.initials: YG
authors.lastname: Liu
mesh_terms: D015153:Blotting, Western; D002465:Cell Movement; D049109:Cell Proliferation; D003092:Colitis / Q000235:genetics / Q000473:pathology*; D003106:Colon / Q000378:metabolism / Q000473:pathology*; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012844:Sincalide / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: C531388:MIRN27 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D012844:Sincalide