title: Overcoming nonviral gene delivery barriers: perspective and future.
abstract: A key end goal of gene delivery research is to develop clinically relevant vectors that can be used to combat elusive diseases such as AIDS. Despite promising engineering strategies, efficiency and ultimately gene modulation efficacy of nonviral vectors have been hindered by numerous in vitro and in vivo barriers that have resulted in subviral performance. In this perspective, we concentrate on the gene delivery barriers associated with the two most common classes of nonviral vectors, cationic-based lipids and polymers. We present the existing delivery barriers and summarize current vector-specific strategies to overcome said barriers.
authors.affiliation: Department of Chemical and Biological Engineering, University at Buffalo, State University of New York , Buffalo, New York, 14260-4200, United States.
authors.forename: Charles H
authors.initials: CH
authors.lastname: Jones
authors.forename: Chih-Kuang
authors.initials: CK
authors.lastname: Chen
authors.forename: Anitha
authors.initials: A
authors.lastname: Ravikrishnan
authors.forename: Snehal
authors.initials: S
authors.lastname: Rane
authors.forename: Blaine A
authors.initials: BA
authors.lastname: Pfeifer
mesh_terms: D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D008055:Lipids / Q000737:chemistry; D011108:Polymers / Q000737:chemistry
chemical_list: D008055:Lipids; D011108:Polymers