title: Crystallization and X-ray diffraction analysis of nylon hydrolase (NylC) from Arthrobacter sp. KI72.
abstract: Nylon hydrolase (NylC) encoded by Arthrobacter plasmid pOAD2 (NylCp2) was expressed in Escherichia coli JM109 and purified by ammonium sulfate fractionation, anion-exchange column chromatography and gel-filtration chromatography. NylCp2 was crystallized by the sitting-drop vapour-diffusion method with ammonium sulfate as a precipitant in 0.1 M HEPES buffer pH 7.5 containing 0.2 M NaCl and 25% glycerol. Diffraction data were collected from the native crystal to a resolution of 1.60 Å. The obtained crystal was spindle shaped and belonged to the C-centred orthorhombic space group C2221, with unit-cell parameters a=70.84, b=144.90, c=129.05 Å. A rotation and translation search gave one clear solution containing two molecules per asymmetric unit.
authors.affiliation: Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nagai
authors.forename: Kengo
authors.initials: K
authors.lastname: Yasuhira
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Dai-ichiro
authors.initials: D
authors.lastname: Kato
authors.forename: Masahiro
authors.initials: M
authors.lastname: Takeo
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Higuchi
authors.forename: Seiji
authors.initials: S
authors.lastname: Negoro
authors.forename: Naoki
authors.initials: N
authors.lastname: Shibata
mesh_terms: D000619:Aminohydrolases / Q000737:chemistry*; D001173:Arthrobacter / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D009757:Nylons / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D009757:Nylons; D000619:Aminohydrolases
keywords: Arthrobacter; nylon hydrolase; plasmid pOAD2