title: Imaging of neutral lipids and neutral lipid associated proteins.
abstract: Intracellular fat droplets are large and have a distinct morphology, which makes their imaging at the light level simple and informative. We detail how to image the fat droplet core by metabolic labeling with fluorescent fatty acids or lipophilic fluorochromes. Further, we describe the use of indirect immunostaining to image fat droplet proteins and fat cores in the same field. We also address the use of appropriate controls for determining signal specificity and other practical considerations for optimizing image quality.
authors.affiliation: Center for Human Nutrition, Washington University School of Medicine, St. Louis, Missouri, USA.
authors.forename: Lydia-Ann L S
authors.initials: LA
authors.lastname: Harris
authors.forename: James R
authors.initials: JR
authors.lastname: Skinner
authors.forename: Nathan E
authors.initials: NE
authors.lastname: Wolins
mesh_terms: D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D005227:Fatty Acids / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism; D002479:Inclusion Bodies / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure*; D050356:Lipid Metabolism; D008055:Lipids; D008565:Membrane Proteins / Q000378:metabolism* / Q000648:ultrastructure; D008856:Microscopy, Fluorescence; D010750:Phosphoproteins / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D005227:Fatty Acids; D005456:Fluorescent Dyes; D008055:Lipids; D008565:Membrane Proteins; D010750:Phosphoproteins
keywords: BODIPY; Immunofluorescence; Immunostaining; Lipid droplets