title: Identification of a soluble isoform of human IL-17RA generated by alternative splicing.
abstract: IL-17RA, a member of the interleukin (IL)-17 receptor family, is a single membrane-spanning protein that ubiquitously expressed on the cell surface. IL-17RA transduces IL-17A, IL-17F, and IL-17A/F heterodimer-mediated signals by forming a complex with IL-17RC, and also signals the IL-17E (also known as IL-25) response in combination with IL-17RB (also known as IL-25R). Previously, soluble isoforms of human IL-17RC and IL-17RB have been reported, but the existence of a soluble isoform of human IL-17RA has remained unclear. Here, we report the identification of a soluble isoform of human IL-17RA at the mRNA and protein levels. Reverse transcribed PCR experiments showed that the IL-17RA variant is generated by spliced out of exon 11 encoding the transmembrane region in a variety of human tissues. The soluble IL-17RA isoform was detected in the culture media of human cell lines by Western blotting. The existence of the soluble IL-17RA isoform sheds new light on the regulation of IL-17RA mediated responses.
authors.affiliation: Division of Oral Biochemistry, Niigata University, Graduate School of Medical and Dental Sciences, 2-5274 Gakkocho-dori, Chuo-ku, Niigata 951-8514, Japan. Electronic address: sohda@dent.niigata-u.ac.jp.
authors.forename: Miwa
authors.initials: M
authors.lastname: Sohda
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Misumi
authors.forename: Kosuke
authors.initials: K
authors.lastname: Tashiro
authors.forename: Manabu
authors.initials: M
authors.lastname: Yamazaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Saku
authors.forename: Kimimitsu
authors.initials: K
authors.lastname: Oda
mesh_terms: D017398:Alternative Splicing; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D005091:Exons / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D053722:Receptors, Interleukin-17 / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D012995:Solubility
chemical_list: C109985:IL17RA protein, human; D020033:Protein Isoforms; D012333:RNA, Messenger; D053722:Receptors, Interleukin-17
keywords: Alternative splicing variant; IL-17RA; Soluble receptor