title: Efficient succinic acid production from lignocellulosic biomass by simultaneous utilization of glucose and xylose in engineered Escherichia coli.
abstract: To enhance succinic acid formation during xylose fermentation in Escherichia coli, overexpression of ATP-forming phosphoenolpyruvate carboxykinase (PEPCK) from Bacillus subtilis 168 in an ldhA, pflB, and ppc deletion strain resulted in a significant increase in cell mass and succinic acid production. However, BA204 displays a low yield of glucose fermentation and sequential glucose-xylose utilization under regulation by the phosphotransferase system (PTS). To improve the capability of glucose fermentation and simultaneously consume sugar mixture for succinic acid production, a pflB, ldhA, ppc, and ptsG deletion strain overexpressing ATP-forming PEPCK, named E. coli BA305, was constructed. As a result, after 120 h fed-batch fermentation of sugarcane bagasse hydrolysate, the dry cell weight and succinic acid concentration in BA305 were 4.58 g L(-1) and 39.3 g L(-1), respectively.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211816, China.
authors.forename: Rongming
authors.initials: R
authors.lastname: Liu
authors.forename: Liya
authors.initials: L
authors.lastname: Liang
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Mingke
authors.initials: M
authors.lastname: Wu
authors.forename: Kequan
authors.initials: K
authors.lastname: Chen
authors.forename: Jiangfeng
authors.initials: J
authors.lastname: Ma
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
authors.forename: Pingkai
authors.initials: P
authors.lastname: Ouyang
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000693:Anaerobiosis; D061249:Batch Cell Culture Techniques; D018533:Biomass; D019149:Bioreactors; D002482:Cellulose / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D005285:Fermentation; D005818:Genetic Engineering; D005947:Glucose / Q000378:metabolism*; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D010730:Phosphoenolpyruvate Carboxylase / Q000378:metabolism; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism; D031786:Saccharum / Q000737:chemistry; D019802:Succinic Acid / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
chemical_list: C036909:lignocellulose; D000255:Adenosine Triphosphate; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D014994:Xylose; D019802:Succinic Acid; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C031629:phosphoenolpyruvate-glucose phosphotransferase; D010730:Phosphoenolpyruvate Carboxylase; D005947:Glucose
keywords: ATP; Escherichia coli; Lignocellulosic hydrolysate; Simultaneous utilization; Succinic acid