title: Preparation and characterization of bimodal porous poly(γ-benzyl-L-glutamate) scaffolds for bone tissue engineering.
abstract: An ideal scaffold in bone tissue-engineering strategy should provide biomimetic extracellular matrix-like architecture and biological properties. Poly(γ-benzyl-L-glutamate) (PBLG) has been a popular model polypeptide for various potential biomedical applications due to its good biocompatibility and biodegradability. This study developed novel bimodal porous PBLG polypeptide scaffolds via a combination of biotemplating method and in situ ring-opening polymerization of γ-benzyl-L-gIutamate N-carboxyanhydride (BLG-NCA). The PBLG scaffolds were characterized by proton nuclear magnetic resonance spectroscopy, X-ray diffraction, differential scanning calorimetry, scanning electron microscope (SEM) and mechanical test. The results showed that the semi-crystalline PBLG scaffolds exhibited an anisotropic porous structure composed of honeycomb-like channels (100-200 μm in diameter) and micropores (5-20 μm), with a very high porosity of 97.4±1.6%. The compressive modulus and glass transition temperature were 402.8±20.6 kPa and 20.2°C, respectively. The in vitro biocompatibility evaluation with MC3T3-E1 cells using SEM, fluorescent staining and MTT assay revealed that the PBLG scaffolds had good biocompatibility and favored cell attachment, spread and proliferation. Therefore, the bimodal porous polypeptide scaffolds are promising for bone tissue engineering.
authors.affiliation: State Key Laboratory for Mechanical Behaviors of Materials, Xi'an Jiaotong University, Xi'an 710049, China. Electronic address: jmqian@mail.xjtu.edu.cn.
authors.forename: Junmin
authors.initials: J
authors.lastname: Qian
authors.forename: Xueqing
authors.initials: X
authors.lastname: Yong
authors.forename: Weijun
authors.initials: W
authors.lastname: Xu
authors.forename: Xinxia
authors.initials: X
authors.lastname: Jin
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D001842:Bone and Bones / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D055119:Elastic Modulus; D051379:Mice; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D023822:Tissue Engineering; D044366:Transition Temperature; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; C003172:poly-gamma-benzyl-L-glutamate; D011099:Polyglutamic Acid
keywords: Bimodal porous structure; Biocompatibility; Biomorphic; In situ polymerization; Poly(γ-benzyl-L-glutamate); Polypeptide scaffold