title: Fluorescence assay for glycan expression on living cancer cells based on competitive strategy coupled with dual-functionalized nanobiocomposites.
abstract: Cell surface glycans are a class of sophisticated biomolecules related to cancer development and progression, and their analysis is of great significance for early cancer diagnosis and treatment. In this paper, we proposed a fluorescence assay to evaluate glycan expression on living cancer cells based on a competitive strategy coupled with dual-functionalized nanobiocomposites. The competitive assay was conducted between living cancer cells and thiomannosyl derivatives using concanavalin A (Con A)-modified electrode as the interaction platform. To impart fluorescence signaling ability to competitive derivatives, quantum dots (QDs) were anchored on BSA-protected Au nanoparticles, and thiomannosyl derivatives were further immobilized on the nanoparticle surface through Au-S binding. Due to the spacing between QDs and Au nanoparticles by BSA, the {QDs-Au-BSA-mannose} nanobiocomposites maintained the fluorescence of QDs and showed binding ability with the Con A-modified electrode. Au nanorods (AuNRs)-modified electrode was used as an effective substrate to immobilize Con A. This assay was successfully applied to the analysis of two cancer cells lines (A549 and QGY-7701). The method is simple and shows promise for the study of glycan expression on living cancer cells.
authors.affiliation: Department of Chemistry, East China Normal University, Shanghai 200062, People's Republic of China. zaxu@chem.ecnu.edu.cn wzhang@chem.ecnu.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Fu
authors.forename: Danqin
authors.initials: D
authors.lastname: Lu
authors.forename: Bin
authors.initials: B
authors.lastname: Lin
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Sun
authors.forename: Kai
authors.initials: K
authors.lastname: Liu
authors.forename: Lili
authors.initials: L
authors.lastname: Xu
authors.forename: Shengping
authors.initials: S
authors.lastname: Zhang
authors.forename: Chen
authors.initials: C
authors.lastname: Hu
authors.forename: Chuangui
authors.initials: C
authors.lastname: Wang
authors.forename: Zhiai
authors.initials: Z
authors.lastname: Xu
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D001681:Biological Assay / Q000379:methods*; D045744:Cell Line, Tumor; D005453:Fluorescence; D006046:Gold / Q000737:chemistry; D006801:Humans; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology; D011134:Polysaccharides / Q000235:genetics* / Q000378:metabolism; D011952:Receptors, Concanavalin A / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D011134:Polysaccharides; D011952:Receptors, Concanavalin A; C027774:concanavalin A-binding glycoproteins; D006046:Gold