title: Probiotic modulation of dendritic cell function is influenced by ageing.
abstract: Dendritic cells (DCs) are critical for the generation of T-cell responses. DC function may be modulated by probiotics, which confer health benefits in immunocompromised individuals, such as the elderly. This study investigated the effects of four probiotics, Bifidobacterium longum bv. infantis CCUG 52486, B. longum SP 07/3, Lactobacillus rhamnosus GG (L.GG) and L. casei Shirota (LcS), on DC function in an allogeneic mixed leucocyte reaction (MLR) model, using DCs and T-cells from young and older donors in different combinations. All four probiotics enhanced expression of CD40, CD80 and CCR7 on both young and older DCs, but enhanced cytokine production (TGF-β, TNF-α) by old DCs only. LcS induced IL-12 and IFNγ production by DC to a greater degree than other strains, while B. longum bv. infantis CCUG 52486 favoured IL-10 production. Stimulation of young T cells in an allogeneic MLR with DC was enhanced by probiotic pretreatment of old DCs, which demonstrated greater activation (CD25) than untreated controls. However, pretreatment of young or old DCs with LPS or probiotics failed to enhance the proliferation of T-cells derived from older donors. In conclusion, this study demonstrates that ageing increases the responsiveness of DCs to probiotics, but this is not sufficient to overcome the impact of immunosenescence in the MLR.
authors.affiliation: Department of Food and Nutritional Sciences, The University of Reading, Reading, UK.
authors.forename: Jialu
authors.initials: J
authors.lastname: You
authors.affiliation: Department of Food and Nutritional Sciences, The University of Reading, Reading, UK.
authors.forename: Honglin
authors.initials: H
authors.lastname: Dong
authors.affiliation: Antigen Presentation Research Group, Imperial College London, Northwick Park and St. Mark's Campus, Harrow, HA1 3UJ, UK.
authors.forename: Elizabeth R
authors.initials: ER
authors.lastname: Mann
authors.affiliation: Antigen Presentation Research Group, Imperial College London, Northwick Park and St. Mark's Campus, Harrow, HA1 3UJ, UK.
authors.forename: Stella C
authors.initials: SC
authors.lastname: Knight
authors.affiliation: Department of Food and Nutritional Sciences, The University of Reading, Reading, UK. Electronic address: p.yaqoob@reading.ac.uk.
authors.forename: Parveen
authors.initials: P
authors.lastname: Yaqoob
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging / Q000276:immunology; D017951:Antigen Presentation; D015703:Antigens, CD / Q000378:metabolism; D001644:Bifidobacterium / Q000276:immunology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D007519:Isoantigens / Q000276:immunology; D007780:Lacticaseibacillus casei / Q000276:immunology*; D052201:Lacticaseibacillus rhamnosus / Q000276:immunology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D019936:Probiotics; D054400:Receptors, CCR7 / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*; D055815:Young Adult
chemical_list: D015703:Antigens, CD; C515370:CCR7 protein, human; D016207:Cytokines; D007519:Isoantigens; D054400:Receptors, CCR7
keywords: Ageing; Allogeneic mixed leucocyte reaction; CFSE; Cytokine; DCs; Dendritic cells; L. casei Shirota; LcS; MLR; PAMPs; PRPs; Probiotics; carboxyfluorescein diacetate succinimidyl ester; dendritic cells; mixed leucocyte reaction; pathogen recognition patterns; pathogen-associated molecular patterns