title: A boy with a tight skin: Borrelia-associated early-onset morphea.
abstract: We present a case of a 16-year-old boy with morphea caused by Borrelia burgdorferi. We re-emphasise an immunohistochemical method, focus floating microscopy (FFM), to detect Borrelia burgdorferi spirochetes in tissue sections. Focus floating microscopy (FFM) proved to be more sensitive than polymerase chain reaction (PCR) and nearly equally specific.
authors.affiliation: Department of Paediatrics, Rijnstate, Arnhem, The Netherlands. roosverberkt@gmail.com.
authors.forename: Roos M
authors.initials: RM
authors.lastname: Verberkt
authors.forename: Matthijs
authors.initials: M
authors.lastname: Janssen
authors.forename: Judit
authors.initials: J
authors.lastname: Wesseling
mesh_terms: D000293:Adolescent; D017668:Age of Onset; D001431:Bacteriological Techniques; D001706:Biopsy; D025065:Borrelia burgdorferi / Q000302:isolation & purification*; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D008193:Lyme Disease / Q000150:complications / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D008297:Male; D011237:Predictive Value of Tests; D012594:Scleroderma, Localized / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D012867:Skin / Q000187:drug effects / Q000382:microbiology* / Q000473:pathology
chemical_list: D007166:Immunosuppressive Agents