title: Mucosal vaccination with recombinant adenovirus encoding nucleoprotein provides potent protection against influenza virus infection.
abstract: Influenza vaccines that target the highly variable surface glycoproteins hemagglutinin and neuraminidase cause inconvenience of having vaccination every year. For this reason, development of universal vaccines targeting conserved viral components is needed. In this study, we generated recombinant adenovirus (rAd) vaccine encoding nucleoprotein (NP) of A/PR/8/34 influenza virus, designated rAd/NP. BALB/c mice were immunized intranasally or sublingually with rAd/NP vaccine and subsequently challenged with lethal doses of heterologous as well as homologous influenza viruses. We found that intranasal immunization of rAd/NP elicited strong mucosal IgA responses as well as stronger CD8 T-cell responses toward immunodominant K(d)-restricted NP147-155 epitope than sublingual immunization. Importantly, only single intranasal but not sublingual immunization of rAd/NP provides potent protection against both homologous and heterologous influenza virus challenges. These results suggest that recombinant rAd/NP could be a universal vaccine candidate for mucosal administration against influenza virus.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, Korea.
authors.forename: So-Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Joo Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Youngjoo
authors.initials: Y
authors.lastname: Choi
authors.forename: Huan H
authors.initials: HH
authors.lastname: Nguyen
authors.forename: Man Ki
authors.initials: MK
authors.lastname: Song
authors.forename: Jun
authors.initials: J
authors.lastname: Chang
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism*; D058956:Administration, Mucosal; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D057809:HEK293 Cells; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000378:metabolism / Q000627:therapeutic use*; D007251:Influenza, Human / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D009698:Nucleoproteins / Q000378:metabolism*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000378:metabolism / Q000627:therapeutic use*
chemical_list: D017931:DNA Primers; D007070:Immunoglobulin A; D007252:Influenza Vaccines; D009698:Nucleoproteins; D014614:Vaccines, Synthetic