title: [Use of recombinant human beta-defensin-3 to evaluate the effect of adhesion of Candida albicans on the surface of soft lining material].
abstract: OBJECTIVE
  To evaluate the effectiveness of recombinant human beta-defensin-3 (rHBD3) on reduction of Candida albicans from the surface of soft lining materials.
  
  
  METHODS
  Specimens made of soft lining materials, which had been contaminated with C. albicans, were immersed in 25 μg/mL rHBD3 and 5.25% sodium hypochlorite (a positive control) for 5, 10, 30 and 60 min. The results were analyzed statistically with SPSS 10.0 software package. Confocal laser scanning microscopy(CLSM) of biofilm-associated C. albicans were treated with rHBD3 in different times.
  
  
  RESULTS
  The results confirmed that an immersion time of 30 min or higher yielded a good disinfection effect, both for the experimental group and the positive control. A large number of pathogenic bacteria were killed and the cells were stained red by SYTO9/PI after 30 min.
  
  
  CONCLUSIONS
  It is possible that soft lining materials immersed in rHBD3 solution that possesses antifungal activity may be clinically protective.
authors.affiliation: .Department of Prosthodontics, Guangdong Provincial Stomatological Hospital, Affiliated Hospital of Southern Medical University. Guangzhou 510280;China.E-mail:83516422@qq.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Shi
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.forename: Xiao-fang
authors.initials: XF
authors.lastname: Liu
authors.forename: Li-min
authors.initials: LM
authors.lastname: Gao
authors.forename: Guang-bao
authors.initials: GB
authors.lastname: Song
mesh_terms: D018441:Biofilms; D002176:Candida albicans; D003822:Denture Liners; D006801:Humans; D012973:Sodium Hypochlorite; D023083:beta-Defensins
chemical_list: C422950:DEFB103A protein, human; D023083:beta-Defensins; D012973:Sodium Hypochlorite