title: Parallel synthesis and splicing redirection activity of cell-penetrating peptide conjugate libraries of a PNA cargo.
abstract: A novel method for the parallel synthesis of peptide-biocargo conjugates was developed that utilizes affinity purification for fast isolation of the conjugates in order to avoid time consuming HPLC purification. The methodology was applied to create two libraries of cell-penetrating peptide (CPP)-PNA705 conjugates from parallel-synthesized peptide libraries. The conjugates were tested for their ability to induce splicing redirection in HeLa pLuc705 cells. The results demonstrate how the novel methodology can be applied for screening purposes in order to find suitable CPP-biocargo combinations and further optimization of CPPs.
authors.affiliation: Medical Research Council, Laboratory of Molecular Biology, Cambridge Biomedical Campus, Francis Crick Avenue, Cambridge, CB2 0QH, UK. mgait@mrc-lmb.cam.ac.uk.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Deuss
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Arzumanov
authors.forename: Donna L
authors.initials: DL
authors.lastname: Williams
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Gait
mesh_terms: D057846:Cell-Penetrating Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006367:HeLa Cells; D006801:Humans; D008968:Molecular Conformation; D019151:Peptide Library; D020135:Peptide Nucleic Acids / Q000737:chemistry* / Q000378:metabolism*; D012326:RNA Splicing / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*
chemical_list: D057846:Cell-Penetrating Peptides; D019151:Peptide Library; D020135:Peptide Nucleic Acids; D012333:RNA, Messenger