title: Stem cell-like dog placenta cells afford neuroprotection against ischemic stroke model via heat shock protein upregulation.
abstract: In this study, we investigated the dog placenta as a viable source of stem cells for stroke therapy. Immunocytochemical evaluation of phenotypic markers of dog placenta cells (DPCs) cultured in proliferation and differentiation medium revealed that DPCs expressed both stem cell and neural cell markers, respectively. Co-culture with DPCs afforded neuroprotection of rat primary neural cells in a dose-dependent manner against oxygen-glucose deprivation. Subsequent in vivo experiments showed that transplantation of DPCs, in particular intravenous and intracerebral cell delivery, produced significant behavioral recovery and reduced histological deficits in ischemic stroke animals compared to those that received intra-arterial delivery of DPCs or control stroke animals. Furthermore, both in vitro and in vivo studies implicated elevated expression of heat shock protein 27 (Hsp27) as a potential mechanism of action underlying the observed therapeutic benefits of DPCs in stroke. This study supports the use of stem cells for stroke therapy and implicates a key role of Hsp27 signaling pathway in neuroprotection.
authors.affiliation: Department of Neurosurgery and Brain Repair, University of South Florida College of Medicine, Tampa, Florida, United States of America.
authors.forename: Seongjin
authors.initials: S
authors.lastname: Yu
authors.forename: Naoki
authors.initials: N
authors.lastname: Tajiri
authors.forename: Nick
authors.initials: N
authors.lastname: Franzese
authors.forename: Max
authors.initials: M
authors.lastname: Franzblau
authors.forename: Eunkyung
authors.initials: E
authors.lastname: Bae
authors.forename: Simon
authors.initials: S
authors.lastname: Platt
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kaneko
authors.forename: Cesar V
authors.initials: CV
authors.lastname: Borlongan
mesh_terms: D000818:Animals; D004285:Dogs; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism*; D007150:Immunohistochemistry; D018696:Neuroprotective Agents; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D051381:Rats; D015398:Signal Transduction / Q000502:physiology*; D033581:Stem Cell Transplantation / Q000379:methods; D013234:Stem Cells / Q000502:physiology*; D020521:Stroke / Q000517:prevention & control*; D015854:Up-Regulation / Q000235:genetics / Q000502:physiology*
chemical_list: D055551:HSP27 Heat-Shock Proteins; D018696:Neuroprotective Agents