title: Ultrasound stimulus to enhance the bone regeneration capability of gelatin cryogels.
abstract: In the present study, gelatin-based cryogels have been seeded with human SAOS-2 osteoblasts. In order to overcome the drawbacks associated with in vitro culture systems, such as limited diffusion and inhomogeneous cell-matrix distribution, this work describes the application of ultrasounds (average power, 149 mW; frequency, 1.5 MHz) to physically enhance the cell culture in vitro. The results indicate that the physical stimulation of cell-seeded gelatin-based cryogels upregulates the bone matrix production.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Fassina
authors.forename: Livia
authors.initials: L
authors.lastname: Visai
authors.forename: Giovanni
authors.initials: G
authors.lastname: Magenes
authors.forename: Jorg
authors.initials: J
authors.lastname: Schelfhout
authors.forename: Nora
authors.initials: N
authors.lastname: Bloise
authors.forename: Federica
authors.initials: F
authors.lastname: Riva
authors.forename: Claudia
authors.initials: C
authors.lastname: Omes
authors.forename: Maria Antonietta
authors.initials: MA
authors.lastname: Avanzini
authors.forename: Maria Gabriella
authors.initials: MG
authors.lastname: Cusella De Angelis
authors.forename: Francesco
authors.initials: F
authors.lastname: Benazzo
authors.forename: Manuel
authors.initials: M
authors.lastname: Dierick
authors.forename: Luc
authors.initials: L
authors.lastname: Van Hoorebeke
authors.forename: Peter
authors.initials: P
authors.lastname: Dubruel
authors.forename: Sandra
authors.initials: S
authors.lastname: Van Vlierberghe
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology*; D002417:Cattle; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D059985:Cryogels / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005780:Gelatin / Q000494:pharmacology*; D006801:Humans; D020100:Hydrogels / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D016062:Porosity / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry; D014465:Ultrasonics; D055114:X-Ray Microtomography
chemical_list: D059985:Cryogels; D020100:Hydrogels; D005780:Gelatin