title: Acute and subchronic toxicity study of tud-rak-ka-sai-puu recipe in rats.
abstract: Acute and subchronic toxicities of Tud-Rak-Ka-Sai-Puu (TR) recipe were studied in male and female rats. After 14 days of a single oral administration of test substance (5,000 mg/kg body weight), measurement of the body and organs weights, necropsy and health monitoring were performed. No signs and differences in the weights and behavior were observed relative to the control rats, suggesting that TR recipe in the dose of 5,000 mg/kg body weight does not produce acute toxicity. The subchronic toxicity was determined by oral feeding in male and female rats daily with the test substance at 2, 20, 200 and 2,000 mg/kg body weight for 90 days. No defects of animal behavior were observed in the test groups. Both test and control groups (on the 90(th) day) as well as the satellite group (on the 118(th) day) were analyzed by measuring their final body and organ weights, taking necropsy, and examining hematology, blood clinical chemistry, and microanatomy. These results together with the information of signs, behavior and health monitoring can lead to a conclusion that an oral administration of TR recipe at 2, 20, 200 and 2,000 mg/kg body weight for 90 days did not cause subchronic toxicity.
authors.affiliation: Division of Pharmacology, Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand.
authors.forename: Seewaboon
authors.initials: S
authors.lastname: Sireeratawong
authors.forename: Urarat
authors.initials: U
authors.lastname: Nanna
authors.forename: Supaporn
authors.initials: S
authors.lastname: Vannasiri
authors.forename: Natthakarn
authors.initials: N
authors.lastname: Chiruntanat
authors.forename: Supachai
authors.initials: S
authors.lastname: Srithiwong
authors.forename: Pennapa
authors.initials: P
authors.lastname: Subcharoen
authors.forename: Pornthip
authors.initials: P
authors.lastname: Termwiset
authors.forename: Parunkul
authors.initials: P
authors.lastname: Tungsukruthai
authors.forename: Anchalee
authors.initials: A
authors.lastname: Chuthaputti
authors.forename: Kanjana
authors.initials: K
authors.lastname: Jaijoy
mesh_terms: D000818:Animals; D005260:Female; D019684:Magnoliopsida / Q000009:adverse effects; D008297:Male; D008519:Medicine, Traditional / Q000009:adverse effects; D008517:Phytotherapy / Q000009:adverse effects; D010936:Plant Extracts / Q000009:adverse effects / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013785:Thailand; D018675:Toxicity Tests
chemical_list: D010936:Plant Extracts
keywords: Acute toxicity; Subchronic toxicity; Tud-Rak-Ka-Sai-Puu Recipe