title: Deoxyribozyme cascade for visual detection of bacterial RNA.
abstract: In the blink of the eye: a cascade of two deoxyribozymes was designed for rapid visual detection of bacterial 16S rRNA. The detection limit is 12.5 ng by the naked eye, with the ability to differentiate between closely related pathogenic and nonpathogenic species.
authors.affiliation: Chemistry Department, University of Central Florida, 4000 Central Florida Blvd, Orlando, FL 32816 (USA).
authors.forename: Yulia V
authors.initials: YV
authors.lastname: Gerasimova
authors.forename: Evan M
authors.initials: EM
authors.lastname: Cornett
authors.forename: Emily
authors.initials: E
authors.lastname: Edwards
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Su
authors.forename: Kyle H
authors.initials: KH
authors.lastname: Rohde
authors.forename: Dmitry M
authors.initials: DM
authors.lastname: Kolpashchikov
mesh_terms: D015100:3,3'-Diaminobenzidine / Q000737:chemistry; D001419:Bacteria / Q000235:genetics*; D001483:Base Sequence; D003124:Colorimetry; D021881:DNA, Catalytic / Q000378:metabolism*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics; D009841:Oligonucleotides / Q000737:chemistry; D012329:RNA, Bacterial / Q000032:analysis*; D012336:RNA, Ribosomal, 16S / Q000032:analysis
chemical_list: D021881:DNA, Catalytic; D009841:Oligonucleotides; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D015100:3,3'-Diaminobenzidine
keywords: RNA recognition; binary deoxyribozyme; mycobacterium tuberculosis; sensors; visual assays