title: Aquimarina amphilecti sp. nov., isolated from the sponge Amphilectus fucorum.
abstract: A Gram-stain-negative, rod-shaped, orange-coloured, catalase- and oxidase-positive, non-motile bacterium, designated strain 92V(T), was isolated from the marine sponge Amphilectus fucorum, collected from Lough Hyne, County Cork, Ireland. 16S rRNA gene sequence analysis revealed that strain 92V(T) clustered with members of the family Flavobacteriaceae, the closest member being Aquimarina latercula NCIMB 1399(T), with a gene sequence similarity of 97.5%. Strain 92V(T) required seawater for growth with optimal growth occurring at 25 °C, at pH 6-7 and with 3% (w/v) NaCl. MK-6 was the sole respiratory quinone present and the major fatty acids were iso-C(17 : 0) 3-OH, iso-C(15 : 0), iso-C(17 : 1)ω9c and iso-C(15 : 0) 3-OH. The DNA G+C content was 36.1 mol%. Combined phenotypic differences and phylogenetic analysis indicate that strain 92V(T) represents a novel species of the genus Aquimarina, for which the name Aquimarina amphilecti sp. nov. is proposed. The type strain is 92V(T) ( = NCIMB 14723(T) = DSM 25232(T)).
authors.affiliation: Marine Biotechnology Centre, Environmental Research Institute, University College Cork, Ireland.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Kennedy
authors.affiliation: Marine Biotechnology Centre, Environmental Research Institute, University College Cork, Ireland.
authors.forename: Lekha Menon
authors.initials: LM
authors.lastname: Margassery
authors.affiliation: Department of Microbiology, University College Cork, Ireland.|Marine Biotechnology Centre, Environmental Research Institute, University College Cork, Ireland.
authors.forename: Niall D
authors.initials: ND
authors.lastname: O'Leary
authors.affiliation: BIOMERIT Research Centre, University College Cork, Ireland.|Department of Microbiology, University College Cork, Ireland.
authors.forename: Fergal
authors.initials: F
authors.lastname: O'Gara
authors.affiliation: Department of Microbiology, University College Cork, Ireland.
authors.forename: John
authors.initials: J
authors.lastname: Morrissey
authors.affiliation: Department of Microbiology, University College Cork, Ireland.|Marine Biotechnology Centre, Environmental Research Institute, University College Cork, Ireland.
authors.forename: Alan D W
authors.initials: ADW
authors.lastname: Dobson
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007494:Ireland; D008969:Molecular Sequence Data; D010802:Phylogeny; D010858:Pigmentation; D011161:Porifera / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6