title: Adipose tissue-derived stem cells treated with estradiol enhance survival of autologous fat transplants.
abstract: Autologous fat transplantation (AFT) is a common and important operation in plastic surgery for soft tissue defects and adipose tissue-derived stem cells (ADSCs) are considered as a promising supplement to decrease absorption and subsequent side effects due to the ability of multiple differentiation and production of vascular endothelial growth factor (VEGF). The capacities of ADSCs can be further enhanced by treatment with 17-β estradiol (E2). Therefore, we hypothesized that E2 may promote the potential of ADSCs for AFT. In this study, ADSCs were extracted from three female patients by liposuction. In vitro studies showed that E2 supplementation at an optimal concentration of 10(-8) M resulted in enhanced proliferation, VEGF production, and adipogenic differentiation of human ADSCs, and reduced apoptosis rate in a serum-free environment. In addition, a nude mice model of fat transplantation was utilized to demonstrate the efficacy of ADSC for survival ratio in vivo. These results using the volume of fat tissues after 12 weeks compared original volume, revealed that the addition of E2-treated ADSCs induced a significantly higher tissue survival ratio (76.9 ± 1.9%) when compared with the ADSC-free system (55.5 ± 1.5%). Furthermore, increased capillary formation stained with hematoxylin-eosin (H&E) was observed in ADSCs systems after treatment with E2. Therefore, this study demonstrated E2 could promote the capacities of ADSCs about aspects of adipogenic differentiation, growth factor secretion and apoptosis reduction in vitro, vascularization improvement in vivo, and then enhanced the survival ratio of AFT.
authors.affiliation: The Plastic and Cosmetic Center, The 1st Affiliated Hospital of Harbin Medical University.
authors.forename: Sai
authors.initials: S
authors.lastname: Luo
authors.forename: Lijun
authors.initials: L
authors.lastname: Hao
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Dongmei
authors.initials: D
authors.lastname: Yu
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Diao
authors.forename: Liwen
authors.initials: L
authors.lastname: Ren
authors.forename: Haiqian
authors.initials: H
authors.lastname: Xu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000637:transplantation*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001391:Azo Compounds; D049109:Cell Proliferation / Q000187:drug effects; D017931:DNA Primers / Q000235:genetics; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006085:Graft Survival / Q000187:drug effects*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000187:drug effects / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D001391:Azo Compounds; D017931:DNA Primers; D042461:Vascular Endothelial Growth Factor A; D004958:Estradiol; C011049:oil red O