title: Canine parvovirus type 2a (CPV-2a)-induced apoptosis in MDCK involves both extrinsic and intrinsic pathways.
abstract: The canine parvovirus type 2 (CPV-2) causes an acute disease in dogs. It has been found to induce cell cycle arrest and DNA damage leading to cellular lysis. In this paper, we evaluated the apoptotic potential of the "new CPV-2a" in MDCK cells and elucidated the mechanism of the induction of apoptosis. The exposure of MDCK cells to the virus was found to trigger apoptotic response. Apoptosis was confirmed by phosphatidylserine translocation, DNA fragmentation assays, and cell cycle analysis. Activation of caspases-3, -8, -9, and -12 and decrease in mitochondrial potential in CPV-2a-infected MDCK cells suggested that the CPV-2a-induced apoptosis is caspase dependent involving extrinsic, intrinsic, and endoplasmic reticulum pathways. Increase in p53 and Bax/Bcl2 ratio was also observed in CPV-2a-infected cells.
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India.
authors.forename: Juwar
authors.initials: J
authors.lastname: Doley
authors.forename: Lakshya Veer
authors.initials: LV
authors.lastname: Singh
authors.forename: G Ravi
authors.initials: GR
authors.lastname: Kumar
authors.forename: Aditya Prasad
authors.initials: AP
authors.lastname: Sahoo
authors.forename: Lovleen
authors.initials: L
authors.lastname: Saxena
authors.forename: Uttara
authors.initials: U
authors.lastname: Chaturvedi
authors.forename: Shikha
authors.initials: S
authors.lastname: Saxena
authors.forename: Rajiv
authors.initials: R
authors.lastname: Kumar
authors.forename: Prafull Kumar
authors.initials: PK
authors.lastname: Singh
authors.forename: R S
authors.initials: RS
authors.lastname: Rajmani
authors.forename: Lakshman
authors.initials: L
authors.lastname: Santra
authors.forename: S K
authors.initials: SK
authors.lastname: Palia
authors.forename: S
authors.initials: S
authors.lastname: Tiwari
authors.forename: D R
authors.initials: DR
authors.lastname: Harish
authors.forename: Arvind
authors.initials: A
authors.lastname: Kumar
authors.forename: G S
authors.initials: GS
authors.lastname: Desai
authors.forename: Smita
authors.initials: S
authors.lastname: Gupta
authors.forename: Shishir K
authors.initials: SK
authors.lastname: Gupta
authors.forename: A K
authors.initials: AK
authors.lastname: Tiwari
mesh_terms: D000818:Animals; D017209:Apoptosis; D001692:Biological Transport; D020169:Caspases / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004171:Diploidy; D004285:Dogs; D004721:Endoplasmic Reticulum / Q000378:metabolism; D061985:Madin Darby Canine Kidney Cells; D009707:Nucleosomes / Q000378:metabolism; D017993:Parvovirus, Canine / Q000502:physiology*; D010718:Phosphatidylserines / Q000378:metabolism; D015398:Signal Transduction; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D009707:Nucleosomes; D010718:Phosphatidylserines; D016159:Tumor Suppressor Protein p53; D020169:Caspases