title: Antigenotoxic activities of the natural dietary coumarins umbelliferone, herniarin and 7-isopentenyloxy coumarin on human lymphocytes exposed to oxidative stress.
abstract: The antigenotoxic effects of umbelliferone (UMB), herniarin (HER) and 7-isopentenyloxy coumarin (7-IP), common natural dietary coumarins, were evaluated on the human lymphocyte DNA damage using single-cell gel electrophoresis. H(2)O(2)-induced DNA break was measured based on the percentage of DNA in tail, and the antigenotoxic effects of the tested compounds were compared with that of ascorbic acid (10, 25, 50, 100 and 200 μM). UMB, HER and 7-IP did not show any genotoxicity, as compared to phosphate-buffered saline. Treatment with UMB, HER and 7-IP led to a significant reduction in the percentage of DNA in tail induced by H(2)O(2) (p < 0.001) at all concentrations. The presence of prenyl moiety in the chemical structure of 7-IP may contribute to its better antigenotoxic property, compared to UMB. The results of this study showed that 7-IP possessed the best antigenotoxic activity among the tested compounds.
authors.affiliation: Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences , Mashhad , Iran .
authors.forename: Ramin
authors.initials: R
authors.lastname: Rezaee
authors.forename: Effat
authors.initials: E
authors.lastname: Behravan
authors.forename: Javad
authors.initials: J
authors.lastname: Behravan
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Soltani
authors.forename: Yazdan
authors.initials: Y
authors.lastname: Naderi
authors.forename: Bahareh
authors.initials: B
authors.lastname: Emami
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Iranshahi
mesh_terms: D000328:Adult; D016587:Antimutagenic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology; D020552:Comet Assay; D003374:Coumarins / Q000008:administration & dosage / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D008214:Lymphocytes / Q000187:drug effects / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D014468:Umbelliferones / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: C461443:7-isopentenyloxycoumarin; D016587:Antimutagenic Agents; D003374:Coumarins; D014468:Umbelliferones; C007929:herniarin; C031477:7-hydroxycoumarin; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid