title: The effects of volatile anesthetics on the extracellular accumulation of [(3)H]GABA in rat brain cortical slices.
abstract: GABA is an inhibitory neurotransmitter that appears to be associated with the action of volatile anesthetics. These anesthetics potentiate GABA-induced postsynaptic currents by synaptic GABAA receptors, although recent evidence suggests that these agents also significantly affect extrasynaptic GABA receptors. However, the effect of volatile anesthetics on the extracellular concentration of GABA in the central nervous system has not been fully established. In the present study, rat brain cortical slices loaded with [(3)H]GABA were used to investigate the effect of halothane and sevoflurane on the extracellular accumulation of this neurotransmitter. The accumulation of [(3)H]GABA was significantly increased by sevoflurane (0.058, 0.11, 0.23, 0.46, and 0.93 mM) and halothane (0.006, 0.012, 0.024, 0.048, 0072, and 0.096 mM) with an EC50 of 0.26 mM and 35 μM, respectively. TTX (blocker of voltage-dependent Na(+) channels), EGTA (an extracellular Ca(2+) chelator) and BAPTA-AM (an intracellular Ca(2+) chelator) did not interfere with the accumulation of [(3)H]GABA induced by 0.23 mM sevoflurane and 0.048 mM halothane. SKF 89976A, a GABA transporter type 1 (GAT-1) inhibitor, reduced the sevoflurane- and halothane-induced increase in the accumulation of GABA by 57 and 63 %, respectively. Incubation of brain cortical slices at low temperature (17 °C), a condition that inhibits GAT function and reduces GABA release through reverse transport, reduced the sevoflurane- and halothane-induced increase in the accumulation of [(3)H]GABA by 82 and 75 %, respectively, relative to that at normal temperature (37 °C). Ouabain, a Na(+)/K(+) ATPase pump inhibitor, which is known to induce GABA release through reverse transport, abolished the sevoflurane and halothane effects on the accumulation of [(3)H]GABA. The effect of sevoflurane and halothane did not involve glial transporters because β-alanine, a blocker of GAT-2 and GAT-3, did not inhibit the effect of the anesthetics. In conclusion, the present study suggests that sevoflurane and halothane increase the accumulation of GABA by inducing the reverse transport of this neurotransmitter. Therefore, volatile anesthetics could interfere with neuronal excitability by increasing the action of GABA on synaptic and extrasynaptic GABA receptors.
authors.affiliation: Programa de Pós-graduação em Medicina Molecular, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Paulo H C
authors.initials: PH
authors.lastname: Diniz
authors.forename: Cristina
authors.initials: C
authors.lastname: Guatimosim
authors.forename: Nancy S
authors.initials: NS
authors.lastname: Binda
authors.forename: Flávia L P
authors.initials: FL
authors.lastname: Costa
authors.forename: Marcus V
authors.initials: MV
authors.lastname: Gomez
authors.forename: Renato S
authors.initials: RS
authors.lastname: Gomez
mesh_terms: D000777:Anesthetics / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism*; D003080:Cold Temperature; D005110:Extracellular Space / Q000187:drug effects* / Q000378:metabolism*; D006221:Halothane / Q000008:administration & dosage / Q000494:pharmacology; D008738:Methyl Ethers / Q000008:administration & dosage / Q000494:pharmacology; D009557:Nipecotic Acids / Q000494:pharmacology; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D000077149:Sevoflurane; D013779:Tetrodotoxin / Q000633:toxicity; D014316:Tritium / Q000378:metabolism; D014835:Volatilization; D015091:beta-Alanine / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D000777:Anesthetics; D008738:Methyl Ethers; D009557:Nipecotic Acids; D014316:Tritium; D015091:beta-Alanine; D000077149:Sevoflurane; D013779:Tetrodotoxin; D005680:gamma-Aminobutyric Acid; D010042:Ouabain; C045598:N-(4,4-diphenyl-3-butenyl)nipecotic acid; D002118:Calcium; D006221:Halothane