title: Morinda citrifolia (Noni) alters oxidative stress marker and antioxidant activity in cervical cancer cell lines.
abstract: BACKGROUND
  Cervical cancer, the second most common cancer in women, has a high mortality rate. Cisplatin, an antitumor agent, is generally used for its treatment. However, the administration of cisplatin is associated with side effects and intrinsic resistance. Morinda citrifolia (Noni), a natural plant product, has been shown to have antioxidant activities in vitro and in vivo.
  
  
  METHODS
  Both HeLa and SiHa cervical cancer cell lines were treated with 10% Noni, 10 mg/dl cisplatin, or a combination of both 10% Noni and 10 mg/dl cisplatin for 24 hours. Post culturing, the cells were pelleted and stored at -70oC for malondialdehyde and catalase assays.
  
  
  RESULTS
  On treatment with Noni, CP, and their combination, the level of MDA decreased by 0.76 fold, 0.49 fold, and 0.68 fold respectively in HeLa cells; and by 0.93 fold, 0.67 fold, and 0.79 fold respectively in SiHa cells, as compared to their controls; whereas catalase activity increased by 1.61 fold, 0.54 fold, and 2.35 fold, respectively in HeLa cells; and by 0.98 fold, 0.39 fold, and 1.85 fold respectively in SiHa cells.
  
  
  CONCLUSIONS
  A decrease in level of lipid peroxidation and an increase in catalase activity were observed with Noni by itself and the effect ameliorated changes observed with cisplatin when given in combination.
authors.affiliation: Department of Biochemistry, All India Institute of Medical Science, New Delhi, India E-mail : rakesh_biochemgupta@yahoo.com.
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Gupta
authors.forename: Neeta
authors.initials: N
authors.lastname: Singh
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D000971:Antineoplastic Combined Chemotherapy Protocols; D000975:Antioxidants / Q000494:pharmacology*; D015153:Blotting, Western; D002374:Catalase / Q000378:metabolism; D002945:Cisplatin / Q000494:pharmacology; D005260:Female; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D032066:Morinda / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D010936:Plant Extracts; D008315:Malondialdehyde; D002374:Catalase; D002945:Cisplatin