title: Fish scale collagen sponge incorporated with Macrotyloma uniflorum plant extract as a possible wound/burn dressing material.
abstract: Application of plant extracts for the burn/wound treatment is followed over the decades as a common practice and it is an important aspect in clinical management. In this study porous collagen sponges (CS) were prepared using fish scales and were incorporated with mupirocin (CSM) and extracts of Macrotyloma uniflorum (CSPE) separately to impart antimicrobial activity to the sponges. The results showed that the addition of plant extract increased the tensile strength of CSPE and stability against collagenase enzyme. FTIR studies have shown the incorporation of plant extract in CSPE, SEM studies have revealed the porous nature of the sponges and XRD patterns have shown the retention of collagen triple helical structure even after the addition of plant extract. CSPE and CSM have exhibited antimicrobial properties. The sponges prepared were analysed for their in vitro biocompatibility studies using fibroblasts and keratinocyte cell lines and the results have shown their biocompatible nature. Based on the results obtained, CS, CSM and CSPE may be tried as a burn/wound dressing materials, initially, in small animals in vivo.
authors.affiliation: Bio-Products Laboratory, Central Leather Research Institute, Adyar, Chennai 600 020, Tamilnadu, India.
authors.forename: Thangavelu
authors.initials: T
authors.lastname: Muthukumar
authors.affiliation: Bio-Products Laboratory, Central Leather Research Institute, Adyar, Chennai 600 020, Tamilnadu, India.
authors.forename: P
authors.initials: P
authors.lastname: Prabu
authors.affiliation: FDDI International Testing Centre, Ministry of Commerce & Industry, Chennai, Tamilnadu, India.
authors.forename: Kausik
authors.initials: K
authors.lastname: Ghosh
authors.affiliation: Bio-Products Laboratory, Central Leather Research Institute, Adyar, Chennai 600 020, Tamilnadu, India. Electronic address: sastrytp@hotmail.com.
authors.forename: Thotapalli Parvathaleswara
authors.initials: TP
authors.lastname: Sastry
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D001672:Biocompatible Materials / Q000737:chemistry; D002056:Burns; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000737:chemistry*; D017364:Collagenases / Q000378:metabolism; D007887:Fabaceae / Q000737:chemistry*; D005399:Fishes; D051379:Mice; D016712:Mupirocin / Q000737:chemistry; D041681:NIH 3T3 Cells; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects; D013718:Tensile Strength; D014945:Wound Healing / Q000187:drug effects*; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D010936:Plant Extracts; D003094:Collagen; D016712:Mupirocin; D017364:Collagenases
keywords: Collagenase; Fish scale collagen; Macrotyloma uniflorum; Mupirocin and sponge; Wound dressing