title: Nanometrology.
abstract: Methods and protocols are described when using fluorescence metrology to determine the average nanoparticle (np) size in colloids in the range of 1-10 nm. The technique is based on determining the rotational correlation time of the np from the decay of fluorescence anisotropy of a dye that is electrostatically or covalently attached to the np as it undergoes Brownian rotation. The np size is then calculated from the Stokes-Einstein equation. The exemplar of silica nps is presented, but the approach can also be applied to other types of nps.
authors.affiliation: The Photophysics Research Group, Department of Physics, Centre for Molecular Nanometrology, SUPA, University of Strathclyde, Glasgow, Scotland, UK.
authors.forename: David J S
authors.initials: DJ
authors.lastname: Birch
authors.forename: Philip
authors.initials: P
authors.lastname: Yip
mesh_terms: D003102:Colloids / Q000737:chemistry; D005453:Fluorescence; D005454:Fluorescence Polarization / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D003102:Colloids; D012822:Silicon Dioxide