title: Telomere length, c-myc and mad-1 expression could represent prognosis markers of myelodysplastic syndrome.
abstract: Telomere dysfunction might generate genomic instability leading to the progression of myelodysplastic syndromes (MDS) into acute myeloid leukemia (AML). We investigated telomere length (TL), telomerase activity (TA) and hTERT, c-myc, mad1, and p53 expression in the bone marrow of patients with MDS (n=109), AML (n=47) and in controls (n=24). TL was lower in MDS patients than in controls (p<0.001) and higher in L-MDS (low, intermediate-1 IPSS, p<0.01) respect H-MDS (high, intermediate-2 IPSS, p<0.01) patients. Mad-1 expression was higher in MDS patients than in controls (p<0.01), c-myc expression was highest in AML and in H-MDS patients. Our results show that the telomere dynamics might be useful for stratifying patients according to a risk scoring system.
authors.affiliation: Clinica di Ematologia, Dipartimento Scienze Cliniche e Molecolari, Ancona, Italy. Electronic address: a.poloni@univpm.it.
authors.forename: Antonella
authors.initials: A
authors.lastname: Poloni
authors.forename: Federica
authors.initials: F
authors.lastname: Serrani
authors.forename: Eleonora
authors.initials: E
authors.lastname: Berardinelli
authors.forename: Giulia
authors.initials: G
authors.lastname: Maurizi
authors.forename: Marianna
authors.initials: M
authors.lastname: Mariani
authors.forename: Benedetta
authors.initials: B
authors.lastname: Costantini
authors.forename: Silvia
authors.initials: S
authors.lastname: Trappolini
authors.forename: Stefania
authors.initials: S
authors.lastname: Mancini
authors.forename: Attilio
authors.initials: A
authors.lastname: Olivieri
authors.forename: Pietro
authors.initials: P
authors.lastname: Leoni
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D015153:Blotting, Western; D016022:Case-Control Studies; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D009190:Myelodysplastic Syndromes / Q000145:classification / Q000235:genetics* / Q000473:pathology; D009687:Nuclear Proteins / Q000235:genetics*; D011379:Prognosis; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019098:Telomerase / Q000235:genetics; D059505:Telomere Homeostasis / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; C501384:MAD1L1 protein, human; C489427:MYC protein, human; D009687:Nuclear Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; C509186:TERT protein, human; D019098:Telomerase
keywords: Acute myeloid leukemia; Myelodysplastic syndromes; Telomerase activity; Telomere length; Transcription factors; hTERT