title: Wide-field fluorescence lifetime imaging with multi-anode detectors.
abstract: Fluorescence lifetime imaging microscopy (FLIM) has become a powerful and widely used tool to monitor inter- and intramolecular dynamics of fluorophore-labeled proteins inside living cells.Here, we present recent achievements in the construction of a positional sensitive wide-field single-photon counting detector system to measure fluorescence lifetimes in the time domain and demonstrate its usage in FRET applications.The setup is based on a conventional fluorescence microscope equipped with synchronized short-pulse lasers that illuminate the entire field of view at minimal invasive intensities, thereby enabling long-term experiments of living cells. The system is capable to acquire single-photon counting images and measures directly the transfer rate of fast photophysical processes as, for instance, FRET, in which it can resolve complex fluorescence decay kinetics.
authors.affiliation: Multidimensional Microscopy and Cellular Diagnostics, Medical Faculty, Otto-von-Guericke-University, Magdeburg, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Hartig
authors.forename: Yury
authors.initials: Y
authors.lastname: Prokazov
authors.forename: Evgeny
authors.initials: E
authors.lastname: Turbin
authors.forename: Werner
authors.initials: W
authors.lastname: Zuschratter
mesh_terms: D004566:Electrodes; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D007700:Kinetics; D008856:Microscopy, Fluorescence / Q000379:methods*; D061848:Optical Imaging; D017785:Photons; D011506:Proteins / Q000032:analysis / Q000737:chemistry
chemical_list: D011506:Proteins