title: Tobacco LSU-like protein couples sulphur-deficiency response with ethylene signalling pathway.
abstract: Most genes from the plant-specific family encoding Response to Low Sulphur (LSU)-like proteins are strongly induced in sulphur (S)-deficient conditions. The exact role of these proteins remains unclear; however, some data suggest their importance for plants' adjustment to nutrient deficiency and other environmental stresses. This work established that the regulation of ethylene signalling is a part of plants' response to S deficiency and showed the interaction between UP9C, a tobacco LSU family member, and one of the tobacco isoforms of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO2A). Increase in ethylene level induced by S deficiency does not take place in tobacco plants with UP9C expressed in an antisense orientation. Based on transcriptomics data, this work also demonstrated that the majority of tobacco's response to S deficiency is misregulated in plants expressing UP9C-antisense. A link between response to S deficiency, ethylene sensing, and LSU-like proteins was emphasized by changes in expression of the genes encoding ethylene receptors and F-box proteins specific for the ethylene pathway.
authors.affiliation: Institute of Biochemistry and Biophysics PAS, Pawinskiego 5A, 02-106 Warsaw, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Moniuszko
authors.forename: Marek
authors.initials: M
authors.lastname: Skoneczny
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Zientara-Rytter
authors.forename: Anna
authors.initials: A
authors.lastname: Wawrzyńska
authors.forename: Dawid
authors.initials: D
authors.lastname: Głów
authors.forename: Simona M
authors.initials: SM
authors.lastname: Cristescu
authors.forename: Frans J M
authors.initials: FJ
authors.lastname: Harren
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Sirko
mesh_terms: D005030:Ethylenes / Q000378:metabolism*; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013455:Sulfur / Q000172:deficiency*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
chemical_list: D005030:Ethylenes; D044783:F-Box Proteins; D010940:Plant Proteins; D013455:Sulfur; C036216:ethylene
keywords: Ethylene; response to nutrients deficit; sulphate; sulphur deficiency; tobacco; transcriptome; transgenic plants.