title: N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes.
abstract: OBJECTIVE
  Oxidative stress plays critical roles in the pathogeneses of diabetes, hypertension, and atherosclerosis, but its effect on fat accumulation is still unclear. In this study, we analyzed the role of the well-known antioxidant and a glutathione (GSH) precursor N-acetylcysteine (NAC) in fat accumulation and the expression of obesity-associated proteins.
  
  
  METHODS
  We studied the effects of 10 µM NAC on obesity-related protein expression in cultured 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids.
  
  
  RESULTS
  NAC treatment inhibited fat accumulation and reduced the expression of obesity-related proteins, including monoamine oxidase A, heat shock protein 70 (HSP70), aminoacylase -1 (ACY-1), and transketolase.
  
  
  CONCLUSIONS
  Our results suggest that the effects of NAC on triglycerides (Tgs) and protein expression are correlated. In support of this, we showed that NAC treatment affected both the Tg synthesis pathway and the expression levels of proteins implicated in human obesity.
authors.affiliation: Universidad de Buenos Aires - IQUIBICEN, CONICET. Intendente Güiraldes 2160, Pabellón 2 (1428), Buenos Aires, Argentina.
authors.forename: P
authors.initials: P
authors.lastname: Calzadilla
authors.forename: M
authors.initials: M
authors.lastname: Gómez-Serrano
authors.forename: E
authors.initials: E
authors.lastname: García-Santos
authors.forename: A
authors.initials: A
authors.lastname: Schiappacasse
authors.forename: Y
authors.initials: Y
authors.lastname: Abalde
authors.forename: J C
authors.initials: JC
authors.lastname: Calvo
authors.forename: B
authors.initials: B
authors.lastname: Peral
authors.forename: L N
authors.initials: LN
authors.lastname: Guerra
mesh_terms: D041721:3T3-L1 Cells; D000111:Acetylcysteine / Q000494:pharmacology*; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D050156:Adipogenesis / Q000187:drug effects*; D000581:Amidohydrolases / Q000096:biosynthesis; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D050556:Fatty Acid-Binding Proteins / Q000096:biosynthesis; D050884:HSP72 Heat-Shock Proteins / Q000096:biosynthesis; D051379:Mice; D008995:Monoamine Oxidase / Q000096:biosynthesis; D018384:Oxidative Stress / Q000187:drug effects; D014174:Transketolase / Q000096:biosynthesis; D014280:Triglycerides / Q000378:metabolism
chemical_list: C493411:Fabp4 protein, mouse; D050556:Fatty Acid-Binding Proteins; D050884:HSP72 Heat-Shock Proteins; D014280:Triglycerides; D008995:Monoamine Oxidase; D014174:Transketolase; D000581:Amidohydrolases; C019672:aminoacylase I; D000111:Acetylcysteine