title: Antioxidative capacity in the fat body of Bombyx mori is increased following oral administration of 4-methylumbelliferone.
abstract: Plant sources of umbelliferones have tumor-inhibitory effects at the cellular level. However, their physiological functions in animals are largely unresolved. In this study, we provide evidence to show that 4-methylumbelliferone (4-MU) participates in the regulation of antioxidative capacity in the fat body of Bombyx mori, a tissue similar to mammalian liver in this model invertebrate. Larvae (3rd day of the 5th instar) were orally exposed to 4 mM 4-MU, an umbelliferone, which swiftly induced the generation of a large number of ROS (e.g. H2O2 increased 6 to 8-fold), and 4-MU was detected in the fat body 8 min after administration. In addition, the activities of CAT and GPx were up-regulated 4 to 11-fold and 2 to 16-fold, respectively, and were helpful in defending fat body cells against oxidative injury in combination with NADPH. Furthermore, significant increases in the contents of T-AOC (up to approx. 2-fold), antioxidants of ASAFR (by 2 to 4-fold) and GSH were detected.
authors.affiliation: Department of Applied Biology, School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou 215123, China; National Engineering Laboratory for Modern Silk, Institute of Agricultural Biotechnology & Ecology, Soochow University, Suzhou 215123, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Fang
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Zhu
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.forename: Hengjiang
authors.initials: H
authors.lastname: Liu
authors.forename: Yue
authors.initials: Y
authors.lastname: He
authors.forename: Xu
authors.initials: X
authors.lastname: Xu
authors.forename: Weimin
authors.initials: W
authors.lastname: Yin
authors.forename: Yanghu
authors.initials: Y
authors.lastname: Sima
authors.forename: Shiqing
authors.initials: S
authors.lastname: Xu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D012831:Bombyx / Q000187:drug effects* / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D005216:Fat Body / Q000187:drug effects* / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D006923:Hymecromone / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D006923:Hymecromone; D009249:NADP; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione
keywords: 4-Methylumbelliferone; 4-methylumbelliferone; 4MU; ASAFR; Anti-oxidant; Bombyx mori; CAT; DEPC; Fat body; Fb; GCL; GPx; GR; GSH; GSSG; NADH oxidase; NOX-1; Oxidative stress response; ROS; Reactive oxygen species; SOD; T-AOC; anti-superoxide anion free radical; catalase; diethyl pyrocarbonate; fat body; glutathione peroxidase; glutathione reductase; oxidized glutathione; reactive oxygen species; reduced glutathione; superoxide dismutase; total antioxidant capacity; γ-glutamate-cysteine ligase