title: Array tomography.
abstract: In array tomography ordered, ribbon-like assemblies of ultrathin serial sections are deposited on a solid substrate and imaged afterwards. The resulting images are then aligned and reconstructed into a three-dimensional representation of the object. Depending on the preparation and labelling regime, different imaging modalities can be applied. When using light microscopy, the labelling with fluorescent markers would be the obvious choice, whereas the imaging in a scanning electron microscope would require impregnation with heavy metals. Depending on preparative constraints, the combination of diverse imaging modalities or truly correlative imaging is possible.
authors.affiliation: Institute for Biological Interfaces 1, Karlsruhe Institute of Technology, Karlsruhe, Germany; HEiKA, Heidelberg-Karlsruhe Research Partnership, Correlative Imaging Platform.
authors.forename: I
authors.initials: I
authors.lastname: Wacker
authors.forename: R R
authors.initials: RR
authors.lastname: Schroeder
mesh_terms: D000818:Animals; D003585:Cell Biology; D003584:Cytological Techniques / Q000379:methods*; D015509:Developmental Biology / Q000379:methods*; D005455:Fluorescent Antibody Technique; D008867:Microtomy; D016610:Tissue Embedding / Q000379:methods; D014054:Tomography / Q000379:methods*; D015027:Zebrafish / Q000033:anatomy & histology
keywords: 3-D reconstruction; Array tomography; correlative imaging