title: Lineage-tracing methods and the kidney.
abstract: The kidney is a complex organ with over 30 different cell types, and understanding the lineage relationships between these cells is challenging. During nephrogenesis, a central question is how the coordinated morphogenesis, growth, and differentiation of distinct cell types leads to development of a functional organ. In mature kidney, understanding cell division and fate during injury, regeneration and aging are critical topics for understanding disease. Genetic lineage tracing offers a powerful tool to decipher cellular hierarchies in both development and disease because it allows the progeny of a single cell, or group of cells, to be tracked unambiguously. Recent advances in this field include the use of inducible recombinases, multicolor reporters, and mosaic analysis. In this review, we discuss lineage-tracing methods focusing on the mouse model system and consider the impact of these methods on our understanding of kidney biology and prospects for future application.
authors.affiliation: 1] Renal Division, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts, USA [2] Harvard Medical School, Boston, Massachusetts, USA [3] Harvard Stem Cell Institute, Cambridge, Massachusetts, USA.
authors.forename: Benjamin D
authors.initials: BD
authors.lastname: Humphreys
authors.affiliation: 1] Renal Division, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts, USA [2] Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Derek P
authors.initials: DP
authors.lastname: DiRocco
mesh_terms: D000818:Animals; D019070:Cell Lineage; D015870:Gene Expression; D017930:Genes, Reporter / Q000235:genetics*; D005821:Genetic Techniques; D007668:Kidney / Q000166:cytology* / Q000196:embryology*; D051379:Mice; D045522:Recombinases / Q000235:genetics*; D011995:Recombination, Genetic
chemical_list: D045522:Recombinases