title: VEGF-mediated proliferation of human adipose tissue-derived stem cells.
abstract: Human adipose tissue-derived stem cells (ADSCs) are an attractive multipotent stem cell source with therapeutic applicability across diverse fields for the repair and regeneration of acute and chronically damaged tissues. In recent years, there has been increasing interest in ADSC for tissue engineering applications. However, the mechanisms underlying the regulation of ADSC proliferation are not fully understood. Here we show that 47 transcripts are up-regulated while 23 are down-regulated in ADSC compared to terminally differentiated cells based on global mRNA profiling and microRNA profiling. Among the up-regulated genes, the expression of vascular endothelial growth factor (VEGF) is fine-tuned by miR-199a-5p. Further investigation indicates that VEGF accelerates ADSC proliferation whereas the multipotency of ADSC remains stable in terms of adipogenic, chondrogenic and osteogenic potentials after VEGF treatment, suggesting that VEGF may serve as an excellent supplement for accelerating ADSC proliferation during in vitro expansion.
authors.affiliation: Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Guangfeng
authors.initials: G
authors.lastname: Chen
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Shi
authors.forename: Chen
authors.initials: C
authors.lastname: Sun
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhou
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Huang
authors.forename: Yu
authors.initials: Y
authors.lastname: Qiu
authors.forename: Xiangyi
authors.initials: X
authors.lastname: Wen
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yushan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shuzhang
authors.initials: S
authors.lastname: Yang
authors.forename: Lixia
authors.initials: L
authors.lastname: Lu
authors.forename: Jieping
authors.initials: J
authors.lastname: Zhang
authors.forename: Qionglan
authors.initials: Q
authors.lastname: Yuan
authors.forename: Jianwei
authors.initials: J
authors.lastname: Lu
authors.forename: Guotong
authors.initials: G
authors.lastname: Xu
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Xue
authors.forename: Zibing
authors.initials: Z
authors.lastname: Jin
authors.forename: Cizhong
authors.initials: C
authors.lastname: Jiang
authors.forename: Ming
authors.initials: M
authors.lastname: Ying
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Liu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D050156:Adipogenesis / Q000187:drug effects; D000273:Adipose Tissue / Q000166:cytology*; D049109:Cell Proliferation / Q000187:drug effects; D020219:Chondrogenesis / Q000187:drug effects; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D023281:Genomics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D042461:Vascular Endothelial Growth Factor A