title: Identification of UV-B-induced microRNAs in wheat.
abstract: MicroRNAs (miRNAs) play critical roles in post-transcriptional gene regulation and act as important endogenous regulators to various stressors. Ultraviolet-B (UV-B) radiation is a major factor influencing crop growth and development. In this study, we isolated a novel wheat miRNA, named Tae-miR6000, and confirmed its expression diversity after UV-B treatments. Additionally, using the Northern blotting technique, we found that six miRNAs were highly responsive to UV-B stress in wheat. Of these six miRNAs, miR159, miR167a, and miR171 were significantly upregulated, and the remaining three miRNAs were downregulated, at different time points after UV-B treatment. This result indicates that miRNAs may be involved in the regulation of targets after induction by UV-B stress. Furthermore, promoter analysis of the UV-B-responsive miRNA genes revealed some light-relevant cis-elements, such as the I-box and G-box. Taken together, the results of this study suggest that wheat miRNAs play important roles in the response to UV-B stress.
authors.affiliation: College of Plant Protection and State Key Laboratory of Crop Stress Biology on Drought Regions, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: B
authors.initials: B
authors.lastname: Wang
authors.forename: Y F
authors.initials: YF
authors.lastname: Sun
authors.forename: N
authors.initials: N
authors.lastname: Song
authors.forename: X J
authors.initials: XJ
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Feng
authors.forename: L L
authors.initials: LL
authors.lastname: Huang
authors.forename: Z S
authors.initials: ZS
authors.lastname: Kang
mesh_terms: D001483:Base Sequence; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D017343:Genes, Plant; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D018749:RNA, Plant / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome; D014908:Triticum / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant