title: Echinacea purpurea root extract enhances the adipocyte differentiation of 3T3-L1 cells.
abstract: Echinacea purpurea has been shown to have anti-diabetic activities; for example, it activates peroxisome proliferator-activated receptor γ (PPARγ) and increases insulin-stimulated glucose uptake. Adipogenesis has been used to study the insulin signaling pathway and to screen anti-diabetic compounds. The present study was conducted to investigate the effects of an ethanol extract of E. purpurea (EEEP) and its constituents on the insulin-induced adipocyte differentiation of 3T3-L1 preadipocytes. When adipocyte differentiation was induced with insulin plus 3-isobutyl-1-methylxanthine and dexamethasone, the accumulation of lipid droplets and the cellular triglyceride content were significantly increased by EEEP. The expressions of PPARγ and C/EBPα in adipocytes treated with EEEP were gradually increased as compared with control cells. Fat accumulation and triglyceride content of adipocytes treated with dodeca-2(E),4(E)-dienoic acid isobutylamide were significantly increased as compared with control cells. The expressions of PPARγ and C/EBPα in adipocytes treated with dodeca-2(E),4(E)-dienoic acid isobutylamide were significantly higher than in control cells. These results suggest EEEP promotes the adipogenesis that is partially induced by insulin and that dodeca-2(E),4(E)-dienoic acid isobutylamide appears to be responsible for EEEP-enhanced adipocyte differentiation.
authors.affiliation: College of Pharmacy and Center for Innovative Cancer Therapeutics, Chungbuk National University, 52 Naesudong-ro, Heungduk-gu, Cheongju, 361-763, Republic of Korea.
authors.forename: Dong-Mi
authors.initials: DM
authors.lastname: Shin
authors.forename: Kyeong-Mi
authors.initials: KM
authors.lastname: Choi
authors.forename: Youn-Sun
authors.initials: YS
authors.lastname: Lee
authors.forename: Wonkyun
authors.initials: W
authors.lastname: Kim
authors.forename: Kyong-Oh
authors.initials: KO
authors.lastname: Shin
authors.forename: Seikwan
authors.initials: S
authors.lastname: Oh
authors.forename: Jae-Chul
authors.initials: JC
authors.lastname: Jung
authors.forename: Mi Kyeong
authors.initials: MK
authors.lastname: Lee
authors.forename: Yong-Moon
authors.initials: YM
authors.lastname: Lee
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.forename: Yeo-Pyo
authors.initials: YP
authors.lastname: Yun
authors.forename: Hwan-Soo
authors.initials: HS
authors.lastname: Yoo
mesh_terms: D041721:3T3-L1 Cells / Q000187:drug effects* / Q000502:physiology; D017667:Adipocytes / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D020900:Echinacea; D051379:Mice; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots
chemical_list: D010936:Plant Extracts