title: Experimental hybridization and backcrossing reveal forces of reproductive isolation in Microbotryum.
abstract: BACKGROUND
  Hybridization and reproductive isolation are central to the origin and maintenance of species, and especially for sympatric species, gene flow is often inhibited through barriers that depend upon mating compatibility factors. The anther-smut fungi (genus Microbotryum) serve as models for speciation in the face of sympatry, and previous studies have tested for but not detected assortative mating. In addition, post-mating barriers are indicated by reduced fitness of hybrids, but sources of those barriers (i.e. ecological maladaptation or genetic incompatibilities) have not yet been detected. Here, backcrossing experiments, specifically controlling for the fungal species origins of the mating compatibility factors, were used to investigate reproductive isolation in the recently-derived species Microbotryum lychnidis-dioicae and Microbotryum silenes-dioicae.
  
  
  RESULTS
  Assortative mating was detected during backcrossing and was manifested by the preferential conjugation of the hybrid-produced gametes with non-hybrid gametes containing mating compatibility factors from the same parental species. Patterns of post-mating performance supported either a level of extrinsic isolation mechanism, where backcross progeny with a higher proportion of the pathogen genome adapted to the particular host environment were favored, or an infection advantage attributed to greater genetic contribution to the hybrid from the M. lychnidis-dioicae genome.
  
  
  CONCLUSIONS
  The use of controlled backcrossing experiments reveals significant species-specific mating type effects on conjugations between recently-derived sister species, which are likely to play important roles in both maintaining species separation and the nature of hybrids lineages that emerge in sympatry between Microbotryum species.
authors.affiliation: Department of Biology, Amherst College, 220 South Pleasant Street, Amherst, MA 01002, USA. Mhood@amherst.edu.
authors.forename: Britta
authors.initials: B
authors.lastname: Büker
authors.forename: Elsa
authors.initials: E
authors.lastname: Petit
authors.forename: Dominik
authors.initials: D
authors.lastname: Begerow
authors.forename: Michael E
authors.initials: ME
authors.lastname: Hood
mesh_terms: D001487:Basidiomycota / Q000145:classification* / Q000166:cytology / Q000235:genetics*; D051456:Gene Flow; D006824:Hybridization, Genetic; D008540:Meiosis; D010944:Plants / Q000382:microbiology; D012098:Reproduction; D060047:Reproductive Isolation