title: Immunochromatographic test for detection of adenovirus from respiratory samples: is it a real solution for pediatric emergency department?
abstract: Rapid diagnosis of adenoviral respiratory infections is required in order to decide optimal treatment strategies. The adenovirus antigen immunochromatography Adeno Respiratory Card Letitest (Leti diagnostics, Barcelona, Spain), was evaluated versus the shell-vial culture and multiplex PCR (Clart Pneumovir Version 3.0, Genomica, Madrid, Spain), in nasopharyngeal washes and oropharyngeal swabs specimens from subjects with respiratory tract infections. Between April 2011 and November 2012, 224 patients were included. The IC Adeno Respiratory Card Letitest was positive for 77.9% (74 of 95) of patients diagnosed at bedside. Using multiplex-PCR as the reference standard, the overall sensitivity was 77.9% and the specificity was 73.6%. Taking shell-viral culture as the reference method, the Adeno Respiratory Card Letitest (Leti diagnostics, Barcelona, Spain) sensitivity and specificity values were 80.0% and 60.9%, respectively. Using RT-PCR (Clart Pneumovir Version 3.0, Genomica, Madrid, Spain) as the reference standard, the viral culture sensitivity was 53.2% and the specificity was 100%. The Adeno Respiratory Card Letitest (Leti diagnostics, Barcelona, Spain) assay could be a simple and rapid method for antigenic detection of adenovirus in pediatric respiratory samples although it would be necessary to improve the specificity and sensitivity of the test.
authors.affiliation: Servicio de Microbiología, Hospital Universitario La Paz, IdiPAZ, Paseo de La Castellana, 261, Madrid 28046, Spain. Electronic address: mpromero.hulp@salud.madrid.org.
authors.forename: María Pilar
authors.initials: MP
authors.lastname: Romero-Gómez
authors.forename: Rosario
authors.initials: R
authors.lastname: López López
authors.forename: Remedios
authors.initials: R
authors.lastname: González Montes
authors.forename: Cristina
authors.initials: C
authors.lastname: Ots Ruiz
authors.forename: Sara
authors.initials: S
authors.lastname: Hierro Cuesta
authors.forename: María Antonia
authors.initials: MA
authors.lastname: Martín Crespo
authors.forename: Santos
authors.initials: S
authors.lastname: García García
mesh_terms: D000258:Adenovirus Infections, Human / Q000175:diagnosis* / Q000821:virology; D000260:Adenoviruses, Human / Q000302:isolation & purification*; D012634:Bodily Secretions / Q000821:virology*; D002648:Child; D002675:Child, Preschool; D002846:Chromatography, Affinity / Q000379:methods*; D004636:Emergency Service, Hospital; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D009305:Nasopharynx / Q000821:virology*; D019095:Point-of-Care Systems; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000821:virology; D012680:Sensitivity and Specificity; D013030:Spain
keywords: Adenovirus; Immunochromatographic test; Respiratory samples