title: Tracking donor RBC survival in premature infants: agreement of multiple populations of biotin-labeled RBCs with Kidd antigen-mismatched RBCs.
abstract: BACKGROUND
  Anemia, a common condition among critically ill premature infants, is affected by red blood cell (RBC) survival (RCS). We hypothesized that transfused allogeneic Kidd antigen-mismatched RBCs would demonstrate the same concurrent RCS tracking as RBCs multilabeled at separate, discrete low densities with biotin (BioRBCs).
  
  
  METHODS
  Allogeneic RBCs from adult donors were labeled at four biotin densities, mixed, and transfused into 17 anemic premature infants. Nine of the donors and neonates were Kidd antigen mismatched. Serial posttransfusion blood samples were assayed for up to 8 wk by flow cytometry to track the survival of the proportions of Kidd antigen-mismatched and Kidd antigen-biotinylated RBCs.
  
  
  RESULTS
  Using linear mixed modeling to compare results, RCS of the three lowest BioRBC densities was similar to RCS by Kidd antigen mismatch and to one another. RCS of RBCs labeled at the highest BioRBC density was shortened.
  
  
  CONCLUSIONS
  RCS of different populations of RBCs can be tracked concurrently and reliably using the three lowest BioRBC densities. Although comparable RCS results can be achieved using Kidd antigen mismatches, BioRBCs are preferred for investigating neonatal anemia because biotin labeling of both allogeneic and autologous RBCs is possible.
authors.affiliation: Department of Pediatrics, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa.
authors.forename: John A
authors.initials: JA
authors.lastname: Widness
authors.affiliation: Department of Pediatrics, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa.
authors.forename: Demet
authors.initials: D
authors.lastname: Nalbant
authors.affiliation: Department of Biochemistry & Molecular Biology and Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas.
authors.forename: Nell I
authors.initials: NI
authors.lastname: Matthews
authors.affiliation: 1] Department of Pediatrics, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa [2] Department of Pathology, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa.
authors.forename: Ronald G
authors.initials: RG
authors.lastname: Strauss
authors.affiliation: Department of Pediatrics, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Schmidt
authors.affiliation: Department of Pediatrics, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa.
authors.forename: Gretchen A
authors.initials: GA
authors.lastname: Cress
authors.affiliation: Department of Biostatistics, University of Iowa College of Public Health, Iowa City, Iowa.
authors.forename: Miriam Bridget
authors.initials: MB
authors.lastname: Zimmerman
authors.affiliation: Department of Biochemistry & Molecular Biology and Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas.
authors.forename: Donald M
authors.initials: DM
authors.lastname: Mock
mesh_terms: D000328:Adult; D001710:Biotin / Q000378:metabolism*; D002470:Cell Survival; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000276:immunology / Q000378:metabolism; D005319:Fetal Hemoglobin / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D007667:Kidd Blood-Group System / Q000276:immunology*
chemical_list: D007667:Kidd Blood-Group System; D001710:Biotin; D005319:Fetal Hemoglobin