title: Sensitive HIV-1 detection in a homogeneous solution based on an electrochemical molecular beacon coupled with a nafion-graphene composite film modified screen-printed carbon electrode.
abstract: A novel electrochemical sensing assay for sensitive determination of HIV-1 in a homogeneous solution has been developed using an electrochemical molecular beacon combined with a nafion-graphene composite film modified screen-printed carbon electrode (nafion-graphene/SPCE). The electrochemical molecular beacon (CAs-MB), comprising a special recognition sequence for the conserved region of the HIV-1 gag gene and a pair of carminic acid molecules as a marker, can indicate the presence of the HIV-1 target by its on/off electrochemical signal behavior. It is suitable for direct, electrochemical determination of HIV-1, thereby simplifying the detection procedure and improving the signal-to-noise (S/N) ratio. To further improve the sensitivity, the nafion-graphene/SPCE was used to monitor changes in the CAs-MB, which has notable advantages, such as being ultrasensitive, inexpensive, and disposable. Under optimized conditions, the peak currents showed a linear relationship with the logarithm of target oligonucleotide concentrations ranging from 40 nM to 2.56 μM, with a detection limit of 5 nM (S/N=3). This sensing assay also displays a good stability, with a recovery of 88-106.8% and RSD<7% (n=5) in real serum samples. This work may lead to the development of an effective method for early point-of-care diagnosis of HIV-1 infection.
authors.affiliation: Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Zhengliang
authors.initials: Z
authors.lastname: Li
authors.forename: Bo
authors.initials: B
authors.lastname: Situ
authors.forename: Zong
authors.initials: Z
authors.lastname: Dai
authors.forename: Qinlan
authors.initials: Q
authors.lastname: Liu
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Dayong
authors.initials: D
authors.lastname: Gu
authors.forename: Lei
authors.initials: L
authors.lastname: Zheng
mesh_terms: D015374:Biosensing Techniques; D002244:Carbon / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D005465:Fluorocarbon Polymers / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D015497:HIV-1 / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D012996:Solutions / Q000737:chemistry
chemical_list: D005465:Fluorocarbon Polymers; D012996:Solutions; C040402:perfluorosulfonic acid; D002244:Carbon; D006108:Graphite
keywords: Electrochemical molecular beacon; Graphene; HIV-1; SPCE; Sensing assay