title: Non-invasive detection of microvascular changes in a paediatric and adolescent population with type 1 diabetes: a pilot cross-sectional study.
abstract: BACKGROUND
  The detection of microvascular damage in type 1 diabetes is difficult and traditional investigations do not detect changes until they are well established. The purpose of this study was to investigate the combined ability of nailfold capillaroscopy, laser Doppler flowmetry, retinal vessel analysis and 24-hr ambulatory blood pressure monitoring to detect early microvascular changes in a paediatric and adolescent population with type 1 diabetes.
  
  
  METHODS
  Patients aged between 8 - 18 years with type I diabetes and no other autoimmune conditions were studied. The participants underwent the above cardiac and vascular investigations in a single three-hour session. Standard parameters including HbA1c were also investigated. Associations between all parameters were described by correlation analysis. Fisher's exact and t-tests determined the association with clinical findings.
  
  
  RESULTS
  26 participants were recruited. The mean HbA1c was 8.1% (SD ± 1.1) with a mean duration of type 1 diabetes of 7.9 years (SD ± 3.4). Three participants had microalbuminuria and one had early signs of retinopathy. Participants with microvascular complications had more avascular areas on nailfold capillaroscopy (p = 0.03). Recent HbA1c was positively associated with the number of nailfold microhaemorrhages (p = 0.03) Decreased baseline perfusion by laser Doppler flowmetry was associated with increased capillary density (p = 0.001) and an increased number of microaneurysms (p = 0.04) on nailfold capillaroscopy.
  
  
  CONCLUSIONS
  This pilot study has shown that in children and adolescents with established type 1 diabetes, abnormal microvasculature can be detected by these investigations. These markers were also positively associated with evidence of suboptimal diabetes control as assessed by HbA1c. Further research will be necessary to determine the practical role of these investigations in the management and progress of the complications of type 1 diabetes.
  
  
  BACKGROUND
  Clinical Trial number NCT01279928, ClinicalTrials.gov.
authors.affiliation: John Hunter Children's Hospital, New Lambton, NSW 2305, Australia. sarahhosking14@gmail.com.
authors.forename: Sarah P M
authors.initials: SP
authors.lastname: Hosking
authors.forename: Rani
authors.initials: R
authors.lastname: Bhatia
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Crock
authors.forename: Ian
authors.initials: I
authors.lastname: Wright
authors.forename: Marline L
authors.initials: ML
authors.lastname: Squance
authors.forename: Glenn
authors.initials: G
authors.lastname: Reeves