title: Dye-attached magnetic poly(hydroxyethyl methacrylate) nanospheres for albumin depletion from human plasma.
abstract: The selective binding of albumin on dye-affinity nanospheres was combined with magnetic properties as an alternative approach for albumin depletion from human plasma. Magnetic poly(hydroxyethyl methacrylate) (mPHEMA) nanospheres were synthesized using mini-emulsion polymerization method in the presence of magnetite powder. The specific surface area of the mPHEMA nanospheres was found to be 1302 m(2)/g. Subsequent to Cibacron Blue F3GA (CB) immobilization onto mPHEMA nanospheres, a serial characterization processing was implemented. The quantity of immobilized CB was calculated as 800 μmol/g. Ultimately, albumin adsorption performance of the CB-attached mPHEMA nanospheres from both aqueous dissolving medium and human plasma were explored.
authors.affiliation: Division of Biochemistry, Department of Chemistry, Hacettepe University , Ankara , Turkey.
authors.forename: Öznur
authors.initials: Ö
authors.lastname: Gökay
authors.forename: Veyis
authors.initials: V
authors.lastname: Karakoç
authors.forename: Müge
authors.initials: M
authors.lastname: Andaç
authors.forename: Deniz
authors.initials: D
authors.lastname: Türkmen
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D004396:Coloring Agents / Q000737:chemistry*; D004655:Emulsions; D006464:Hemoperfusion / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D059346:Magnets; D054118:Nanospheres / Q000737:chemistry* / Q000648:ultrastructure; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D058105:Polymerization; D012709:Serum Albumin / Q000737:chemistry*; D013696:Temperature; D014227:Triazines / Q000737:chemistry*
chemical_list: D004396:Coloring Agents; D004655:Emulsions; D012709:Serum Albumin; D014227:Triazines; D011102:Polyhydroxyethyl Methacrylate; C012842:Cibacron Blue F 3GA
keywords: PHEMA; albumin depletion; dye-ligand affinity; magnetic nanospheres; proteomics