title: Reduction in heat shock protein 90 correlates to neuronal vulnerability in the rat piriform cortex following status epilepticus.
abstract: In the present study, we addressed the question of whether the distinct patterns of heat shock protein (HSP) 70 and HSP90 expressions in the brain region represents the regional specific responses to status epilepsticus (SE) in an effort to better understand the role of HSPs in epileptogenic insult. HSP70 immunoreactivity was increased in CA3 pyramidal cells as well as dentate granule cells at 12h-1week after SE. HSP70 immunoreactivity was transiently increased in neurons within the piriform cortex (PC) following SE. Linear regression analysis showed no correlation between the intensity of NeuN and that of HSP70. In contrast to HSP70, HSP90 immunoreactivity was decreased in CA1-3 pyramidal cells at 4days-4weeks after SE. In addition, HSP90 immunoreactivity was decreased in PC neurons at 12h-4weeks after SE. linear regression analysis showed a direct proportional relationship between the intensity of NeuN and that of HSP90. Therefore, these findings suggest that HSP90 degradation may be closely related to neuronal vulnerability to SE insult.
authors.affiliation: Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chunchon 200-702, South Korea; Institute of Epilepsy Research, College of Medicine, Hallym University, Chunchon 200-702, South Korea.
authors.forename: Y-J
authors.initials: YJ
authors.lastname: Kim
authors.forename: J-Y
authors.initials: JY
authors.lastname: Kim
authors.forename: A-R
authors.initials: AR
authors.lastname: Ko
authors.forename: T-C
authors.initials: TC
authors.lastname: Kang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002540:Cerebral Cortex / Q000378:metabolism*; D004195:Disease Models, Animal; D005455:Fluorescent Antibody Technique; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000096:biosynthesis; D018841:HSP90 Heat-Shock Proteins / Q000032:analysis / Q000096:biosynthesis*; D006624:Hippocampus / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013226:Status Epilepticus / Q000378:metabolism*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins
keywords: EDTA; FJB; Fluoro-Jade B; HSF1; HSP; HSP70; HSP90; PB; PBS; PC; SE; epilepsy; ethylenediaminetetraacetic acid; heat shock factor 1; heat shock protein; hippocampus; phosphate buffer; phosphate-buffered saline; piriform cortex; priform cortex; status epilepticus