title: [Interest of the study of a not identified peak on a hemoglobin chromatogram: delta variant or inter-sample contaminations?].
abstract: D-10(®) (Bio-Rad) analyzer using cationic exchange high performance chromatography (HPLC) allows the detection of the main hemoglobin variants. This observation shows the presence of a peak on chromatogram with a low intensity and no quantifiable which can lead to different diagnosis. Inter-sample contaminations can be confused with the presence of an hemoglobin variant. This case highlights the importance of the knowledge of technicals limits for validation and clinical use of results.
authors.affiliation: Laboratoire de biologie et de recherche pédiatriques, American Memorial Hospital, CHU de Reims, France.
authors.forename: Aurore
authors.initials: A
authors.lastname: Desmons
authors.forename: Stéphane
authors.initials: S
authors.lastname: Jaisson
authors.forename: Philippe
authors.initials: P
authors.lastname: Gillery
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Guillard
mesh_terms: D001774:Blood Chemical Analysis / Q000592:standards; D001800:Blood Specimen Collection / Q000592:standards*; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards; D059018:DNA Contamination; D003951:Diagnostic Errors; D005189:False Positive Reactions; D014644:Genetic Variation; D006451:Hemoglobin, Sickle / Q000032:analysis* / Q000235:genetics*; D006454:Hemoglobins / Q000032:analysis* / Q000235:genetics; D006801:Humans
chemical_list: D006451:Hemoglobin, Sickle; D006454:Hemoglobins
keywords: HPLC; HbA1c; HbS; inter-sample contaminations