title: Screening and characterization of extracelluar L-asparaginase producing Bacillus subtilis strain hswx88, isolated from Taptapani hotspring of Odisha, India.
abstract: OBJECTIVE
  To screen and isolate an eco-friendly, a thermophilic and potent L-asparaginase producing bacterium, with novel immunological properties that may obviates hypersensitivity reactions.
  
  
  METHODS
  In the present study bacterial strain isolated for extracellular L-asparaginase production from hotspring, identified by morphological, biochemical and physiological tests followed by 16S rDNA technology and the L-asparaginase production ability was tested by both semi quantitative and quantitative enzymatic assay.
  
  
  RESULTS
  The bacterial strain was identified as Bacillus subtilis strain hswx88 (GenBank Accession Number: JQ237656.1). The extracellular enzyme yielding capacity isolate Bacillus subtilis strain hswx88 (23.8 IU/mL) was found to be 1.7 and 14.5 times higher than the reference organism Pectobacterium carotovorum MTCC 1428 (14.2 IU/mL) and Bacillus sp. BCCS 034 (1.64 IU/mL).
  
  
  CONCLUSIONS
  The isolate is eco-friendly and useful to produce bulk quantity of extracellular, thermophilic L-asparaginase for the treatment of various tumor cases and for preparation of acrylamide free fry food preparation.
authors.affiliation: Pharmaceutical Biotechnology Division, University Department of Pharmaceutical Sciences, Utkal University, Vani Vihar, Bhubaneswar, Odisha-751 004, India.
authors.forename: Biswaprakash
authors.initials: B
authors.lastname: Pradhan
authors.forename: Sashi K
authors.initials: SK
authors.lastname: Dash
authors.forename: Sabuj
authors.initials: S
authors.lastname: Sahoo
mesh_terms: D001215:Asparaginase / Q000378:metabolism*; D001412:Bacillus subtilis / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D045482:Hot Springs / Q000382:microbiology*; D006801:Humans; D007194:India; D008853:Microscopy; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D001215:Asparaginase
keywords: 16S rDNA; Bacillus subtilis strain hswx88; L-asparaginase; Thermophilic bacteria