title: Development, purification, and characterization of monoclonal antibodies against recombinant histidine-rich protein 3 of Plasmodium falciparum.
abstract: In the present study, monoclonal antibodies (MAbs) against recombinant histidine-rich protein (rHRP3) were developed and assessed for their potential in detection of Plasmodium falciparum HRP3. Hybridomas were obtained by fusion of Sp2/0 mouse myeloma cells and spleen cells from the mouse immunized with purified rHRP3. Three MAbs (IgG1 isotype) specific to rHRP3 were established and characterized by enzyme-linked immunosorbent assay (ELISA) and immunoblotting for sensitivity and specificity. Purification of MAbs from hybridoma cell culture supernatant and PAbs from rabbit anti-serum were carried out using Phenylpropylamine (PPA) HyperCel(™) sorbent. The MAbs were able to detect rHRP3 and the HRP3 from P. falciparum spent medium. Sandwich ELISA was developed to quantify HRP3 in the spent medium of P. falciparum culture. The generated MAbs could be potentially used in immuno-based diagnostic systems for the detection of P. falciparum HRP.
authors.affiliation: Centre for Bioseparation Technology, VIT University , Vellore, Tamil Nadu, India .
authors.forename: B
authors.initials: B
authors.lastname: Palani
authors.forename: S Shiva
authors.initials: SS
authors.lastname: Ranjini
authors.forename: N S
authors.initials: NS
authors.lastname: Jayaprakash
authors.forename: M A
authors.initials: MA
authors.lastname: Vijayalakshmi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000235:genetics; D000953:Antigens, Protozoan / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006825:Hybridomas / Q000166:cytology / Q000378:metabolism; D015151:Immunoblotting; D008288:Malaria / Q000175:diagnosis*; D051379:Mice; D010963:Plasmodium falciparum / Q000276:immunology* / Q000378:metabolism*; D015800:Protozoan Proteins / Q000276:immunology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology*; D012680:Sensitivity and Specificity; D013154:Spleen / Q000166:cytology; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; C578339:HRP3 protein, Plasmodium falciparum; D015800:Protozoan Proteins; D011994:Recombinant Proteins