title: Endosymbiotic microorganisms in Adelges (Sacchiphantes) viridis (Insecta, Hemiptera, Adelgoidea: Adelgidae): Molecular characterization, ultrastructure and transovarial transmission.
abstract: The aim of this paper was to identify endosymbiotic microorganisms living in the body cavity of a Polish population of an aphid, Adelges (Sacchiphantes) viridis, as well as to describe their ultrastructure and mode of transmission between generations. Molecular data (amplification and sequencing of 16S rRNA genes) indicated that endosymbionts of A. (S.) viridis are Betaproteobacteria of the species "Candidatus Vallotia virida". Endosymbiotic bacteria are rod-shaped and localized in the cytoplasm of specific cells, termed bacteriocytes, of host insects. Endosymbionts sharing the same bacteriocytes differ in the density of their cytoplasm. There are two morphotypes of endosymbiotic bacteria: with electron-dense cytoplasm and electron-translucent cytoplasm. Since only bacteria containing electron-dense cytoplasm were observed in the binary fusion stage, differences in density of the cytoplasm are probably due to changes in the cytoskeleton of bacteria during division. Endosymbionts of A. (S.) viridis are transovarially (i.e. via oocytes) transmitted from the mother to the offspring.
authors.affiliation: Department of Developmental Biology and Morphology of Invertebrates, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Kraków, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Michalik
authors.affiliation: Department of Genetics and Evolution, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Kraków, Poland.
authors.forename: Aniela
authors.initials: A
authors.lastname: Gołas
authors.affiliation: Department of Developmental Biology and Morphology of Invertebrates, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Kraków, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Kot
authors.affiliation: Department of Zoology, Faculty of Biology and Environmental Protection, University of Silesia, Bankowa 9, 40-007 Katowice, Poland.
authors.forename: Karina
authors.initials: K
authors.lastname: Wieczorek
authors.affiliation: Department of Developmental Biology and Morphology of Invertebrates, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Kraków, Poland. Electronic address: teresa.szklarzewicz@uj.edu.pl.
authors.forename: Teresa
authors.initials: T
authors.lastname: Szklarzewicz
mesh_terms: D000818:Animals; D001042:Aphids / Q000145:classification / Q000382:microbiology* / Q000648:ultrastructure; D020562:Betaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D046529:Microscopy, Electron, Transmission; D009865:Oocytes / Q000382:microbiology / Q000648:ultrastructure; D013559:Symbiosis
keywords: 16S rRNA gene; Adelgids; Endosymbiotic microorganisms; Transovarial transmission of endosymbionts