title: Long-term experiment to study the development, interaction, and influencing factors of DEXA parameters.
abstract: Dual-energy X-ray absorption (DEXA) is commonly used to measure bone mineral density (BMD), bone mineral content (BMC), and body composition data (fat mass and lean mass) for phenotype assessment in mice. We were interested in the long-term development of BMD, BMC, lean mass, and fat mass of mice, also taking into account sex and genetic background. The dataset was used to analyze correlations among the different parameters. We analyzed males and females from inbred strains C3HeB/FeJ and C57BL/6J, starting from 42 until 528 days of age. To evaluate the effect of husbandry systems, we repeated a part of the study in a second facility with a different caging system. We also assessed different DEXA settings and repeatability of the scans. The results of this study were used to draw conclusions for the use of DEXA analysis in mouse phenotyping approaches.
authors.affiliation: Helmholtz Zentrum München, German Mouse Clinic, Institute of Experimental Genetics, German Research Center for Environmental Health (GmbH), Ingolstädter Landstr. 1, 85764, Neuherberg, Germany, hfuchs@helmholtz-muenchen.de.
authors.forename: Helmut
authors.initials: H
authors.lastname: Fuchs
authors.forename: Christine
authors.initials: C
authors.lastname: Gau
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Hans
authors.forename: Valerie
authors.initials: V
authors.lastname: Gailus-Durner
authors.forename: Martin
authors.initials: M
authors.lastname: Hrabě de Angelis
mesh_terms: D015502:Absorptiometry, Photon; D050154:Adiposity / Q000235:genetics; D000822:Animal Husbandry; D000818:Animals; D015519:Bone Density / Q000235:genetics*; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000378:metabolism*; D005260:Female; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D015203:Reproducibility of Results; D015430:Weight Gain