title: Fluorescence-based method for assessment of blood-brain barrier disruption.
abstract: We report on a fluorescence-based optical method for assessment of blood-brain barrier in humans. The technique is based on monitoring of fluorescence light excited in the dye circulating in the brain. Measurements were carried out in healthy volunteers and in patients with disruption of the blood-brain barrier with the use of time-resolved method during inflow and washout of indocyanine green after its intravenous injection. We show large differences in the fluorescence signals - in healthy subjects a fast washout of the dye can be observed whereas in patients the washout is significantly prolonged. We conclude that the monitoring of the fluorescence signals during injection of exogenous optical contrast agent can be used for the assessment of the condition of blood-brain barrier at the bedside. The technique may be of benefit for diagnosis of the patients suffering from damage of the blood-brain barrier and in monitoring of therapies used in such patients.
authors.forename: Adam
authors.initials: A
authors.lastname: Liebert
authors.forename: Daniel
authors.initials: D
authors.lastname: Milej
authors.forename: Wojciech
authors.initials: W
authors.lastname: Weigl
authors.forename: Anna
authors.initials: A
authors.lastname: Gerega
authors.forename: Michal
authors.initials: M
authors.lastname: Kacprzak
authors.forename: Roman
authors.initials: R
authors.lastname: Maniewski
mesh_terms: D001783:Blood Flow Velocity / Q000502:physiology; D001812:Blood-Brain Barrier; D001921:Brain / Q000187:drug effects; D001927:Brain Diseases / Q000503:physiopathology; D003287:Contrast Media; D004566:Electrodes; D004569:Electroencephalography / Q000295:instrumentation / Q000379:methods; D005456:Fluorescent Dyes / Q000008:administration & dosage*; D064368:Healthy Volunteers; D006801:Humans; D007208:Indocyanine Green; D007275:Injections, Intravenous; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D055100:Optical Fibers; D056888:Patient Positioning; D013194:Staining and Labeling
chemical_list: D003287:Contrast Media; D005456:Fluorescent Dyes; D007208:Indocyanine Green