title: Ursodeoxycholic and deoxycholic acids: A good and a bad bile acid for intestinal calcium absorption.
abstract: The aim of this study was to investigate the effect of ursodeoxycholic acid (UDCA) on intestinal Ca(2+) absorption and to find out whether the inhibition of this process caused by NaDOC could be prevented by UDCA. Chicks were employed and divided into four groups: (a) controls, (b) treated with 10mM NaDOC, (c) treated with 60 μg UDCA/100g of b.w., and (d) treated with 10mM NaDOC and 60 μg UDCA/100g of b.w. UDCA enhanced intestinal Ca(2+) absorption, which was time and dose-dependent. UDCA avoided the inhibition of intestinal Ca(2+) absorption caused by NaDOC. Both bile acids altered protein and gene expression of molecules involved in the transcellular pathway of intestinal Ca(2+) absorption, but in the opposite way. UDCA aborted the oxidative stress produced by NaDOC in the intestine. UDCA and UDCA plus NaDOC increased vitamin D receptor protein expression. In conclusion, UDCA is a beneficial bile acid for intestinal Ca(2+) absorption. Contrarily, NaDOC inhibits the intestinal cation absorption through triggering oxidative stress. The use of UDCA in patients with cholestasis would be benefited because of the protective effect on the intestinal Ca(2+) absorption, avoiding the inhibition caused by hydrophobic bile acids and neutralizing the oxidative stress.
authors.affiliation: Laboratorio "Dr. Fernando Cañas", Cátedra De Bioquímica Y Biología Molecular, Facultad De Ciencias Médicas, INICSA (CONICET-Universidad Nacional De Córdoba), Pabellón Argentina, 2do. Piso, Ciudad Universitaria, 5000 Córdoba, Argentina.
authors.forename: Valeria
authors.initials: V
authors.lastname: Rodríguez
authors.forename: María
authors.initials: M
authors.lastname: Rivoira
authors.forename: Ana
authors.initials: A
authors.lastname: Marchionatti
authors.forename: Adriana
authors.initials: A
authors.lastname: Pérez
authors.forename: Nori
authors.initials: N
authors.lastname: Tolosa de Talamoni
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002645:Chickens; D003840:Deoxycholic Acid / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005786:Gene Expression Regulation / Q000187:drug effects; D007408:Intestinal Absorption / Q000187:drug effects*; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D013997:Time Factors; D014580:Ursodeoxycholic Acid / Q000494:pharmacology*
chemical_list: D003840:Deoxycholic Acid; D014580:Ursodeoxycholic Acid; D000469:Alkaline Phosphatase; D002118:Calcium
keywords: Ca(2+)-ATPase; Calbindin D(28k); Intestinal Ca(2+) absorption; Na(+)/Ca(2+) exchanger; NaDOC; UDCA