title: Kinetic framework of spindle assembly checkpoint signalling.
abstract: The mitotic spindle assembly checkpoint (SAC) delays anaphase onset until all chromosomes have attached to both spindle poles. Here, we investigated SAC signalling kinetics in response to acute detachment of individual chromosomes using laser microsurgery. Most detached chromosomes delayed anaphase until they had realigned to the metaphase plate. A substantial fraction of cells, however, entered anaphase in the presence of unaligned chromosomes. We identify two mechanisms by which cells can bypass the SAC: first, single unattached chromosomes inhibit the anaphase-promoting complex/cyclosome (APC/C) less efficiently than a full complement of unattached chromosomes; second, because of the relatively slow kinetics of re-imposing APC/C inhibition during metaphase, cells were unresponsive to chromosome detachment up to several minutes before anaphase onset. Our study defines when cells irreversibly commit to enter anaphase and shows that the SAC signal strength correlates with the number of unattached chromosomes. Detailed knowledge about SAC signalling kinetics is important for understanding the emergence of aneuploidy and the response of cancer cells to chemotherapeutics targeting the mitotic spindle.
authors.affiliation: 1] Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), 1030 Vienna, Austria [2] Institute of Biochemistry, ETH Zurich, 8093 Zurich, Switzerland [3] Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA.
authors.forename: Amalie E
authors.initials: AE
authors.lastname: Dick
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Gerlich
mesh_terms: D002877:Chromosomes, Human; D006801:Humans; D018386:Kinetochores; D059566:M Phase Cell Cycle Checkpoints; D015398:Signal Transduction; D008941:Spindle Apparatus