title: Enterovirus 74 infection in children.
abstract: Enterovirus 74 (EV74) is a rarely detected viral infection of children. In 2010, EV74 was identified in New Zealand in a 2 year old child with acute flaccid paralysis (AFP) through routine polio AFP surveillance. A further three cases of EV74 were identified in children within six months. These cases are the first report of EV74 in New Zealand. In this study we describe the near complete genome sequence of four EV74 isolates from New Zealand, which shows only limited sequence identity in the non-structural proteins when compared to the other two known EV74 sequences. As is typical of enteroviruses multiple recombination events were evident, particularly in the P2 region and P3 regions. This is the first complete EV74 genome sequenced from a patient with acute flaccid paralysis.
authors.affiliation: Clinical Virology, The Institute of Environmental Science and Research, National Centre for Biosecurity and Infectious Disease, Wellington, New Zealand.
authors.forename: Matthew
authors.initials: M
authors.lastname: Peacey
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Hall
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Angela K
authors.initials: AK
authors.lastname: Todd
authors.forename: Seiha
authors.initials: S
authors.lastname: Yen
authors.forename: Jasmine
authors.initials: J
authors.lastname: Chan-Hyams
authors.forename: Christy J
authors.initials: CJ
authors.lastname: Rand
authors.forename: Jo-Ann
authors.initials: JA
authors.lastname: Stanton
authors.forename: Q Sue
authors.initials: QS
authors.lastname: Huang
mesh_terms: D000367:Age Factors; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D004770:Enterovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000453:epidemiology*; D005243:Feces / Q000821:virology; D005260:Female; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008969:Molecular Sequence Data; D009520:New Zealand / Q000453:epidemiology; D010641:Phenotype; D010802:Phylogeny