title: Molecular phylogenetic analysis of Orientia tsutsugamushi based on the groES and groEL genes.
abstract: DNA sequences encoding the GroES and GroEL proteins of Orientia tsutsugamushi were amplified by the PCR and sequenced. Pairwise alignment of full-length groES and groEL gene sequences indicated high sequence similarity (90.4-100% and 90.3-100%) in O. tsutsugamushi, suggesting that these genes are good candidates for the molecular diagnosis and phylogenetic analysis of scrub typhus. Comparisons of the 56-kD type-specific antigen (TSA) protein gene and the groES and groEL genes showed that genotypes based on the 56-kD TSA gene were not related to a cluster containing the groES and groEL genes in a dendrogram, suggesting that a gene rearrangement may be associated with homologous recombination in mites.
authors.affiliation: 1 Infectious Disease Surveillance Center, National Institute of Infectious Diseases , Tokyo, Japan .
authors.forename: Satoru
authors.initials: S
authors.lastname: Arai
authors.forename: Kenji
authors.initials: K
authors.lastname: Tabara
authors.forename: Norishige
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Hiromi
authors.initials: H
authors.lastname: Fujita
authors.forename: Asao
authors.initials: A
authors.lastname: Itagaki
authors.forename: Marina
authors.initials: M
authors.lastname: Kon
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Satoh
authors.forename: Kazuko
authors.initials: K
authors.lastname: Araki
authors.forename: Keiko
authors.initials: K
authors.lastname: Tanaka-Taya
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Takada
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yoshikawa
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ishihara
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Okabe
authors.forename: Kazunori
authors.initials: K
authors.lastname: Oishi
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D018835:Chaperonin 10 / Q000235:genetics*; D018834:Chaperonin 60 / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D012285:Orientia tsutsugamushi / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012612:Scrub Typhus / Q000175:diagnosis / Q000382:microbiology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D018835:Chaperonin 10; D018834:Chaperonin 60; D004269:DNA, Bacterial