title: Microfluidic array with integrated oxygenation control for real-time live-cell imaging: effect of hypoxia on physiology of microencapsulated pancreatic islets.
abstract: In this article, we present a novel microfluidic islet array based on a hydrodynamic trapping principle. The lab-on-a-chip studies with live-cell multiparametric imaging allow understanding of physiological and pathophysiological changes of microencapsulated islets under hypoxic conditions. Using this microfluidic array and imaging analysis techniques, we demonstrate that hypoxia impairs the function of microencapsulated islets at the single islet level, showing a heterogeneous pattern reflected in intracellular calcium signaling, mitochondrial energetic, and redox activity. Our approach demonstrates an improvement over conventional hypoxia chambers that is able to rapidly equilibrate to true hypoxia levels through the integration of dynamic oxygenation. This work demonstrates the feasibility of array-based cellular analysis and opens up new modality to conduct informative analysis and cell-based screening for microencapsulated pancreatic islets.
authors.affiliation: Department of Surgery/Transplant, University of Illinois at Chicago , 840 South Wood Street, Room 502, Chicago, Illinois 60612.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Nourmohammadzadeh
authors.forename: Joe F
authors.initials: JF
authors.lastname: Lo
authors.forename: Matt
authors.initials: M
authors.lastname: Bochenek
authors.forename: Joshua E
authors.initials: JE
authors.lastname: Mendoza-Elias
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Ze
authors.initials: Z
authors.lastname: Li
authors.forename: Liyi
authors.initials: L
authors.lastname: Zeng
authors.forename: Merigeng
authors.initials: M
authors.lastname: Qi
authors.forename: David T
authors.initials: DT
authors.lastname: Eddington
authors.forename: José
authors.initials: J
authors.lastname: Oberholzer
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D015687:Cell Hypoxia / Q000502:physiology; D003199:Computer Systems; D004339:Drug Compounding / Q000379:methods; D006801:Humans; D007515:Islets of Langerhans / Q000502:physiology*; D044085:Microfluidics / Q000379:methods*; D010101:Oxygen Consumption / Q000502:physiology*; D051381:Rats