title: 3D super-resolution imaging with blinking quantum dots.
abstract: Quantum dots are promising candidates for single molecule imaging due to their exceptional photophysical properties, including their intense brightness and resistance to photobleaching. They are also notorious for their blinking. Here we report a novel way to take advantage of quantum dot blinking to develop an imaging technique in three-dimensions with nanometric resolution. We first applied this method to simulated images of quantum dots and then to quantum dots immobilized on microspheres. We achieved imaging resolutions (fwhm) of 8-17 nm in the x-y plane and 58 nm (on coverslip) or 81 nm (deep in solution) in the z-direction, approximately 3-7 times better than what has been achieved previously with quantum dots. This approach was applied to resolve the 3D distribution of epidermal growth factor receptor (EGFR) molecules at, and inside of, the plasma membrane of resting basal breast cancer cells.
authors.affiliation: Department of Physics, ‡Center for Physics of the Living Cells, and §Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign , Urbana, Illinois 61801, United States.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Gilbert
authors.initials: G
authors.lastname: Fruhwirth
authors.forename: En
authors.initials: E
authors.lastname: Cai
authors.forename: Tony
authors.initials: T
authors.lastname: Ng
authors.forename: Paul R
authors.initials: PR
authors.lastname: Selvin
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism; D002138:Calibration; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000378:metabolism; D005260:Female; D006801:Humans; D045663:Quantum Dots
chemical_list: D066246:ErbB Receptors