title: Influence of the application of chelant EDDS on soil enzymatic activity and microbial community structure.
abstract: The present study evaluated the effects of a biodegradable chelant, S,S-ethylenediaminedisuccinic acid (EDDS), on enzyme activities and microbial community composition in copper (Cu)-contaminated soils, planted with either corn or beans. Results showed that the application of EDDS did not affect urease and acid phosphatase activities in the soil, but greatly reduced catalase and saccharase activities, and increased β-glucosidase activity on the seventh day after EDDS application. On the 28th day, no significant difference was observed in the enzyme activities (except for β-glucosidase) of EDDS-treated soils compared to the controls. Analysis of phospholipid fatty acids (PLFAs) showed that the application of 3 mmol kg(-1) EDDS did not cause significant stress to soil microbial communities. However, PCR-denaturing gradient gel electrophoresis (PCR-DGGE) fingerprint revealed that EDDS influenced the bacterial communities in the soils, and the effects on bean soils were more significant than that with corn. In general, the enzyme activities and bacterial communities were influenced by the application of EDDS, but the impact became weaker or even disappeared with the biodegradation of EDDS.
authors.affiliation: College of Life Sciences, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing 210095, China; Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Guiping
authors.initials: G
authors.lastname: Wang
authors.forename: Zhineng
authors.initials: Z
authors.lastname: Cheng
authors.forename: Yue
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Shen
authors.forename: Chunling
authors.initials: C
authors.lastname: Luo
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D002374:Catalase / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology*; D003300:Copper; D004269:DNA, Bacterial / Q000032:analysis; D005029:Ethylenediamines / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D005658:Fungi / Q000378:metabolism; D027805:Phaseolus / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants; D013386:Succinates / Q000494:pharmacology*; D014510:Urease / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism; D043324:beta-Fructofuranosidase / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D002614:Chelating Agents; D004269:DNA, Bacterial; D005029:Ethylenediamines; D005227:Fatty Acids; D012989:Soil Pollutants; D013386:Succinates; C041447:N,N'-ethylenediamine disuccinic acid; D003300:Copper; D002374:Catalase; D000135:Acid Phosphatase; D001617:beta-Glucosidase; D043324:beta-Fructofuranosidase; D014510:Urease
keywords: Copper; EDDS; Enzyme activities; Microbial community