title: Label-free monitoring of whole cell vitality.
abstract: The Intelligent Mobile Lab (IMOLA) delivers metabolic and morphological parameters of living cells in a label-free and real time way. It represents a key technology for the development of new cell-based assays. Electrochemical microsensors are used to measure the extracellular acidification (pH), cellular respiration (pO2), changes in cell number and morphology (electric impedance) in a controlled environment. These parameters are closely linked to the intracellular signaling network of the living cells. They are thus likely to respond sensitively to changes in cellular vitality. A wide spectrum of cell types can be tested with the system, including adherent and suspended cells, continuous cell lines, primary cells or tissue samples. The platform is described in detail and applications in the field's oncology, toxicology and environmental monitoring are shown.
authors.forename: D
authors.initials: D
authors.lastname: Weiss
authors.forename: M
authors.initials: M
authors.lastname: Brischwein
authors.forename: H
authors.initials: H
authors.lastname: Grothe
authors.forename: B
authors.initials: B
authors.lastname: Wolf
authors.forename: J
authors.initials: J
authors.lastname: Wiest
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002452:Cell Count; D002460:Cell Line; D045744:Cell Line, Tumor; D002516:Ceramics; D002708:Chlorella; D002945:Cisplatin / Q000737:chemistry; D003584:Cytological Techniques; D017097:Electric Impedance; D004563:Electrochemistry / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods*; D005898:Glass; D005947:Glucose / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D010100:Oxygen / Q000737:chemistry; D010641:Phenotype; D015398:Signal Transduction; D012825:Silicon / Q000737:chemistry; D013696:Temperature; D015262:Xenobiotics
chemical_list: D015262:Xenobiotics; D005947:Glucose; D002945:Cisplatin; D010100:Oxygen; D012825:Silicon