title: Using eggshell membrane as nerve guide channels in peripheral nerve regeneration.
abstract: OBJECTIVE
  The aim of this study was to evaluate the final outcome of nerve regeneration across the eggsell membrane (ESM) tube conduit in comparison with autograft.
  
  
  METHODS
  Thirty adult male rats (250-300 g) were randomized into (1) ESM conduit, (2) autograft, and (3) sham surgery groups. The eggs submerged in 5% acetic acid. The decalcifying membranes were cut into four pieces, rotated over the teflon mandrel and dried at 37(°)C. The left sciatic nerve was surgically cut. A 10-mm nerve segment was cut and removed. In the ESM group, the proximal and distal cut ends of the sciatic nerve were telescoped into the nerve guides. In the autograft group, the 10 mm nerve segment was reversed and used as an autologous nerve graft. All animals were evaluated by sciatic functional index (SFI) and electrophysiology testing.
  
  
  RESULTS
  The improvement in SFI from the first to the last evalution in ESM and autograft groups were evaluated. On days 49 and 60 post-operation, the mean SFI of ESM group was significantly greater than the autograft group (P< 0.05). On day 90, the mean nerve conduction velocity (NCV) of ESM group was greater than autograft group, although the difference was not statistically significant (P> 0.05).
  
  
  CONCLUSIONS
  These findings demonstrate that ESM effectively enhances nerve regeneration and promotes functional recovery in injured sciatic nerve of rat.
authors.affiliation: Neurophysiology Research Center, Department of Anatomy, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
authors.forename: Gholam Hossein
authors.initials: GH
authors.lastname: Farjah
authors.forename: Behnam
authors.initials: B
authors.lastname: Heshmatian
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Karimipour
authors.forename: Ali
authors.initials: A
authors.lastname: Saberi
keywords: Eggshell membrane; Nerve regeneration; Rat; Sciatic nerve