title: Impact of cold ischemia on cytokines after partial liver transplantation in rats.
abstract: To study the impact of cold ischemia on tumor necrosis factor-alpha (TNF-α) and interleukin-10 (IL-10) expression after liver transplantation, a stable model of partial liver transplantation in rats was established. The experimental animals were divided into the following groups: a partial hepatectomy control group, a group that received partial liver transplantation after 30 min of cold ischemia (experimental group A), and a group that received a partial liver transplantation after 10 h of cold ischemia (experimental group B). The survival rate was observed in each group. The liver tissue was sampled 1, 2, and 4 days after surgery, and immunohistochemical detection of proliferating cell nuclear antigen TNF-α and IL-10 was performed. The correlation between liver regeneration and TNF-α and IL-10 expression was analyzed, and the impact of the 2 cytokines on rat liver regeneration after liver transplantation was evaluated. The survival rates of rats in the partial hepatectomy control group, in the group that received a partial liver transplantation after 30 min of cold ischemia, and the group that received a partial liver transplantation after 10 h of cold ischemia were 100, 70, and 33.3%, respectively. The expression of proliferating cell nuclear antigen and TNF-α was decreased (P < 0.05), and IL-10 expression was increased (P < 0.05) in animals that received a partial liver transplant after 10 h of cold ischemia compared with that in the animals that received a partial liver transplant after 30 min of cold ischemia. We conclude that with the extension of cold ischemic time, liver regeneration and survival rate after liver transplantation decreased. TNF-α and IL-10 play important regulatory roles in the regeneration process of transplanted livers.
authors.affiliation: Southwest Hospital & Institute of Hepatobilitary Surgery, Third Military Medical University, Chongqing, China.
authors.forename: Q-A
authors.initials: QA
authors.lastname: Qi
authors.forename: Z-Y
authors.initials: ZY
authors.lastname: Yang
authors.forename: K-S
authors.initials: KS
authors.lastname: Ma
authors.forename: Q
authors.initials: Q
authors.lastname: Lu
authors.forename: S-G
authors.initials: SG
authors.lastname: Wang
authors.forename: X-W
authors.initials: XW
authors.lastname: Li
authors.forename: F
authors.initials: F
authors.lastname: Xia
authors.forename: W
authors.initials: W
authors.lastname: Liu
authors.forename: P
authors.initials: P
authors.lastname: Bie
mesh_terms: D000818:Animals; D050377:Cold Ischemia / Q000009:adverse effects*; D006498:Hepatectomy; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000473:pathology; D008115:Liver Regeneration; D016031:Liver Transplantation / Q000379:methods*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10