title: Different virulence of porcine and porcine-like bovine rotavirus strains with genetically nearly identical genomes in piglets and calves.
abstract: Direct interspecies transmissions of group A rotaviruses (RVA) have been reported under natural conditions. However, the pathogenicity of RVA has never been directly compared in homologous and heterologous hosts. The bovine RVA/Cow-tc/KOR/K5/2004/G5P[7] strain, which was shown to possess a typical porcine-like genotype constellation similar to that of the G5P[7] prototype RVA/Pig-tc/USA/OSU/1977/G5P9[7] strain, was examined for its pathogenicity and compared with the porcine G5P[7] RVA/Pig-tc/KOR/K71/2006/G5P[7] strain possessing the same genotype constellation. The bovine K5 strain induced diarrhea and histopathological changes in the small intestine of piglets and calves, whereas the porcine K71 strain caused diarrhea and histopathological changes in the small intestine of piglets, but not in calves. Furthermore, the bovine K5 strain showed extra-intestinal tropisms in both piglets and calves, whereas the porcine K71 strain had extra-intestinal tropisms in piglets, but not in calves. Therefore, we performed comparative genomic analysis of the K71 and K5 RVA strains to determine whether specific mutations could be associated with these distinct clinical and pathological phenotypes. Full-length sequencing analyses for the 11 genomic segments for K71 and K5 revealed that these strains were genetically nearly identical to each other. Two nucleotide mutations were found in the 5' untranslated region (UTR) of NSP5 and the 3' UTR of NSP3, and eight amino acid mutations in VP1-VP4 and NSP2. Some of these mutations may be critical molecular determinants for RVA virulence and/or pathogenicity.
authors.affiliation: Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Republic of Korea. jelle.matthijnssens@uz.kuleuven.ac.be.
authors.forename: Jun-Gyu
authors.initials: JG
authors.lastname: Park
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jelle
authors.initials: J
authors.lastname: Matthijnssens
authors.forename: Mia Madel
authors.initials: MM
authors.lastname: Alfajaro
authors.forename: Deok-Song
authors.initials: DS
authors.lastname: Kim
authors.forename: Kyu-Yeol
authors.initials: KY
authors.lastname: Son
authors.forename: Hyoung-Jun
authors.initials: HJ
authors.lastname: Kwon
authors.forename: Myra
authors.initials: M
authors.lastname: Hosmillo
authors.forename: Eun-Hye
authors.initials: EH
authors.lastname: Ryu
authors.forename: Ji-Yun
authors.initials: JY
authors.lastname: Kim
authors.forename: Rohani B
authors.initials: RB
authors.lastname: Cena
authors.forename: Ju-Hwan
authors.initials: JH
authors.lastname: Lee
authors.forename: Mun-Il
authors.initials: MI
authors.lastname: Kang
authors.forename: Sang-Ik
authors.initials: SI
authors.lastname: Park
authors.forename: Kyoung-Oh
authors.initials: KO
authors.lastname: Cho
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D003967:Diarrhea / Q000662:veterinary* / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012401:Rotavirus / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology; D016415:Sequence Alignment / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D014774:Virulence