title: A novel alkaloid from marine-derived actinomycete Streptomyces xinghaiensis with broad-spectrum antibacterial and cytotoxic activities.
abstract: Due to the increasing emergence of drug-resistant bacteria and tumor cell lines, novel antibiotics with antibacterial and cytotoxic activities are urgently needed. Marine actinobacteria are rich sources of novel antibiotics, and here we report the discovery of a novel alkaloid, xinghaiamine A, from a marine-derived actinomycete Streptomyces xinghaiensis NRRL B24674(T). Xinghaiamine A was purified from the fermentation broth, and its structure was elucidated based on extensive spectroscopic analysis, including 1D and 2D NMR spectrum as well as mass spectrometry. Xinghaiamine A was identified to be a novel alkaloid with highly symmetric structure on the basis of sulfoxide functional group, and sulfoxide containing compound has so far never been reported in microorganisms. Biological assays revealed that xinghaiamine A exhibited broad-spectrum antibacterial activities to both Gram-negative persistent hospital pathogens (e.g. Acinetobacter baumannii, Pseudomonas aeruginosa and Escherichia coli) and Gram-positive ones, which include Staphylococcus aureus and Bacillus subtilis. In addition, xinghaiamine A also exhibited potent cytotoxic activity to human cancer cell lines of MCF-7 and U-937 with the IC50 of 0.6 and 0.5 µM, respectively.
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian, China.
authors.forename: Wence
authors.initials: W
authors.lastname: Jiao
authors.forename: Fenghua
authors.initials: F
authors.lastname: Zhang
authors.forename: Xinqing
authors.initials: X
authors.lastname: Zhao
authors.forename: Jiehan
authors.initials: J
authors.lastname: Hu
authors.forename: Joo-Won
authors.initials: JW
authors.lastname: Suh
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D059001:Aquatic Organisms / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D005285:Fermentation; D006801:Humans; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D013302:Streptomyces / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000470:Alkaloids; D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; C587994:xinghaiamine A