title: Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy.
abstract: We have developed a fast hardware friendly bi-exponential fluorescence lifetime algorithm suitable for 2D CMOS single-photon avalanche diode (SPAD) arrays. The performance of the proposed algorithm against other techniques is demonstrated on the data from a plant specimen (Convallaria) by using 0.13µm CMOS SPAD arrays mounted on a multi-beam multi-photon microscopy system.
authors.forename: D D-U
authors.initials: DD
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Poland
authors.forename: S
authors.initials: S
authors.lastname: Coelho
authors.forename: D
authors.initials: D
authors.lastname: Tyndall
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Richardson
authors.forename: R K
authors.initials: RK
authors.lastname: Henderson
authors.forename: S M
authors.initials: SM
authors.lastname: Ameer-Beg
mesh_terms: D000465:Algorithms; D031405:Convallaria / Q000502:physiology*; D004867:Equipment Design; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation* / Q000379:methods; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods; D008027:Light; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D009010:Monte Carlo Method; D061848:Optical Imaging; D017785:Photons; D025941:Protein Interaction Mapping; D012666:Semiconductors; D012815:Signal Processing, Computer-Assisted