title: Broad-spectrum activity of a novel antibiotic peptide against multidrug-resistant veterinary isolates.
abstract: The emergence of multidrug resistant (MDR) and extensively drug resistant (XDR) bacteria has become a medical and veterinary problem. Antimicrobial peptides (AMPs) show potential to overcome antibiotic resistance and could be used therapeutically. A novel AMP (AMP2041) was developed in silico and its microbiocidal activity against MDR clinical strains isolated from cattle (n=6), dogs (n=8), and pigs (n=20) was evaluated. AMP2041 showed strong antimicrobial activity against all Gram-positive and Gram-negative MDR clinical strains tested. Within 20 min of incubation, there was complete killing of Pseudomonas aeruginosa ATCC 27953 and a 90% reduction of colony count for Escherichia coli ATCC 25922. For Staphylococcus aureus ATCC 25923, a 90% reduction of colony count was observed within 120 min of incubation.
authors.affiliation: Dipartimento di Scienze Medico-Veterinarie, Università degli Studi di Parma, via del Taglio 10, 43126 Parma, Italy. Electronic address: clotildesilvia.cabassi@unipr.it.
authors.forename: Clotilde Silvia
authors.initials: CS
authors.lastname: Cabassi
authors.forename: Simone
authors.initials: S
authors.lastname: Taddei
authors.forename: Sandro
authors.initials: S
authors.lastname: Cavirani
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Baroni
authors.forename: Paolo
authors.initials: P
authors.lastname: Sansoni
authors.forename: Antonello A
authors.initials: AA
authors.lastname: Romani
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy* / Q000382:microbiology; D004283:Dog Diseases / Q000188:drug therapy* / Q000382:microbiology; D004285:Dogs; D024901:Drug Resistance, Multiple, Bacterial; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D013552:Swine; D013553:Swine Diseases / Q000188:drug therapy* / Q000382:microbiology
chemical_list: C000618832:AMP2041; D023181:Antimicrobial Cationic Peptides
keywords: Antibiotic peptides; Antimicrobial resistance; MDR bacteria; Veterinary isolates