title: Dihydroavenanthramide D prevents UV-irradiated generation of reactive oxygen species and expression of matrix metalloproteinase-1 and -3 in human dermal fibroblasts.
abstract: Ultraviolet B (UVB) radiation induces photoageing by upregulating the expression of matrix metalloproteinases (MMPs) in human skin cells. Dihydroavenanthramide D (DHAvD) is a synthetic analog to naturally occurring avenanthramide, which is the active component in oats. Although anti-inflammatory, anti-atherosclerotic and antioxidant effects have been reported, the antiphotoageing effects of DHAvD are yet to be understood. In this study, we investigated the inhibitory effects of DHAvD on UVB-induced production of reactive oxygen species (ROS) and expression of MMPs, and its molecular mechanism in UVB-irradiated human dermal fibroblasts. Western blot and real-time PCR analyses revealed that DHAvD inhibited UVB-induced MMP-1 and MMP-3 expression. It also significantly blocked UVB-induced ROS generation in fibroblasts. Additionally, DHAvD attenuated UVB-induced phosphorylation of MAPKs, activation of NF-κB and AP-1. DHAvD regulates UVB-irradiated MMP expression by inhibiting ROS-mediated MAPK/NF-κB and AP-1 activation. DHAvD may be a useful candidate for preventing UV light-induced skin photoageing.
authors.affiliation: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, South Korea.
authors.forename: Jeong-Mi
authors.initials: JM
authors.lastname: Kim
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Noh
authors.forename: Kang-Beom
authors.initials: KB
authors.lastname: Kwon
authors.forename: Bo-Mi
authors.initials: BM
authors.lastname: Hwang
authors.forename: Jin-Ki
authors.initials: JK
authors.lastname: Hwang
authors.forename: Yong-Ouk
authors.initials: YO
authors.lastname: You
authors.forename: Min Seuk
authors.initials: MS
authors.lastname: Kim
authors.forename: Wan
authors.initials: W
authors.lastname: Lee
authors.forename: Jeong-Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Hye-Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jong-Suk
authors.initials: JS
authors.lastname: Kim
authors.forename: Young-Rae
authors.initials: YR
authors.lastname: Lee
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry; D002467:Cell Nucleus / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism*; D010766:Phosphorylation; D017382:Reactive Oxygen Species; D015398:Signal Transduction; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D015595:Skin Aging / Q000187:drug effects*; D014466:Ultraviolet Rays; D062367:ortho-Aminobenzoates / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D017382:Reactive Oxygen Species; D062367:ortho-Aminobenzoates; C532345:dihydroavenanthramide D; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1
keywords: DHAvD; MAPK; MMP; ROS; UVB