title: Diaphorobacter aerolatus sp. nov., isolated from air, and emended description of the genus Diaphorobacter.
abstract: A Gram-stain-negative, non-motile, aerobic rod, designated 8604S-37(T), was isolated from an outdoor air sample collected in Suwon region, Republic of Korea. The isolate was catalase-positive and oxidase-negative. 16S rRNA gene sequence analysis indicated that strain 8604S-37(T) was most closely related to Diaphorobacter nitroreducens NA10B(T) and Diaphorobacter oryzae RF3(T), with which it shared 97.0% 16S rRNA gene sequence similarity. Strain 8604S-37(T) grew at 10-37 °C (optimum 28 °C; no growth occurred at 4 or 40 °C) and pH 5-9 (optimum pH 7). The strain tolerated up to 3% NaCl (w/v) (optimum 0%). The only respiratory quinone was ubiquinone-8 (Q-8). The polar lipid profile comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and one unknown aminolipid. The major fatty acids were C16 : 0, summed feature 3 (comprising C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH), summed feature 8 (C(18 : 1)ω6c and/or C(18 : 1)ω7c) and C(17 : 0) cyclo, and the predominant hydroxy fatty acid was C(10 : 0) 3-OH. Strain 8604S-37(T) exhibited less than 50% DNA-DNA relatedness with Diaphorobacter nitroreducens KACC 13856(T) and Diaphorobacter oryzae KACC 16857(T). The DNA G+C content was 65 mol%. Strain 8604S-37(T) represents a novel species of the genus Diaphorobacter, for which the name Diaphorobacter aerolatus sp. nov. is proposed. The type strain is 8604S-37(T) ( = KACC 16536(T) = NBRC 108926(T)). An emended description of the genus Diaphorobacter is also proposed.
authors.affiliation: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Moon
authors.affiliation: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Jae-Hyung
authors.initials: JH
authors.lastname: Ahn
authors.affiliation: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Hang-Yeon
authors.initials: HY
authors.lastname: Weon
authors.affiliation: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Seung-Beom
authors.initials: SB
authors.lastname: Hong
authors.affiliation: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Soon-Ja
authors.initials: SJ
authors.lastname: Seok
authors.affiliation: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Soon-Wo
authors.initials: SW
authors.lastname: Kwon
mesh_terms: D000391:Air Microbiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D042621:Comamonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8