title: Screening of a protein that interacts with the matrix attachment region-binding protein from Dunaliella salina.
abstract: We isolated the matrix attachment region-binding protein (MBP) DMBP-1 from Dunaliella salina in our previous studies. MBPs are part of the cis-acting protein family cluster. The regulatory function possibly works through the interaction of the MBPs with each other. In the present study, DMBP-1 was used as the bait in screening the D. salina cDNA library for DMBP-1 interactors that could potentially mediate the DMBP-1-regulated functions. A novel MBP, namely, DMBP-2, was identified as a DMBP-1 binding partner. The cDNA of DMBP-1 was 823 bp long and contained a 573 bp open reading frame, which encoded a polypeptide of 191 amino acids. The interaction between DMBP-2 and DMBP-1 was further confirmed through glutathione S-transferase pull-down assays.
authors.affiliation: Department of Life Science and Technology, Xinxiang Medical University, Xinxiang, Henan 453003, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.forename: Zhaoxi
authors.initials: Z
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Lin
authors.forename: Baosheng
authors.initials: B
authors.lastname: Yang
authors.forename: Tianyun
authors.initials: T
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000235:genetics*; D000460:Chlorophyta / Q000235:genetics*; D003001:Cloning, Molecular; D015723:Gene Library; D045170:Matrix Attachment Regions / Q000235:genetics*
chemical_list: D002352:Carrier Proteins