title: The influence of perfusate volume on antimicrobial concentration in synovial fluid following intravenous regional limb perfusion in the standing horse.
abstract: This study investigated the influence of perfusate volume on antimicrobial concentration in synovial fluid following intravenous regional limb perfusion (IVRLP) and assessed the efficacy of low volume IVRLP. The front limbs of 9 horses were randomly assigned to 1 of 3 volume groups: 10 mL (Group 1), 30 mL (Group 2), or 60 mL (Group 3). A tourniquet was applied distal to the carpus and the limbs were perfused with 500 mg genta-micin diluted to the assigned volume via a catheter placed in the lateral palmar digital vein at the level of the proximal sesamoid bones. Synovial fluid samples were collected from the metacarpophalangeal joint at 30 minutes, followed by removal of the tourniquet. Gentamicin concentration in synovial fluid was detected using a fluorescence polarization immunoassay. There were no statistically significant differences among gentamicin concentrations in synovial fluid among perfusate volume groups. Mean gentamicin concentration in Group 1 (125.9 μg/mL) was higher than Group 2 (82.7 μg/mL) and Group 3 (56.1 μg/mL).
authors.affiliation: Peterson and Smith Equine Hospital, 4747 SW 60th Avenue, Ocala, Florida 34474, USA.
authors.forename: Rosaleen M
authors.initials: RM
authors.lastname: Hyde
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Lynch
authors.forename: Carol K
authors.initials: CK
authors.lastname: Clark
authors.forename: Donnie E
authors.initials: DE
authors.lastname: Slone
authors.forename: Faith E
authors.initials: FE
authors.lastname: Hughes
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005260:Female; D005552:Forelimb; D005839:Gentamicins / Q000008:administration & dosage / Q000493:pharmacokinetics*; D006736:Horses; D010477:Perfusion / Q000379:methods / Q000662:veterinary; D013582:Synovial Fluid / Q000737:chemistry*; D014018:Tissue Distribution
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins