title: O-glycosylation of the non-canonical T-cadherin from rabbit skeletal muscle by single mannose residues.
abstract: O-mannosylation is a vital protein modification. In humans, defective O-mannosylation of α-dystroglycan results in severe congenital muscular dystrophies. However, other proteins bearing this modification in vivo are still largely unknown. Here, we describe a highly reliable method combining glycosidase treatment with LC-MS analyses to identify mammalian O-mannosylated proteins from tissue sources. Our workflow identified T-cadherin (H-cadherin, CDH13) as a novel O-mannosylated protein. In contrast to known O-mannosylated proteins, single mannose residues (Man-α-Ser/Thr) are attached to this cell adhesion molecule. Conserved O-glycosylation sites in T-, E- and N-cadherins from different species, point to a general role of O-mannosyl glycans for cadherin function.
authors.affiliation: Centre for Organismal Studies Heidelberg, University of Heidelberg, Im Neuenheimer Feld 360, 69120 Heidelberg, Germany; Center for Molecular Biology, University of Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany.
authors.forename: Patrick R
authors.initials: PR
authors.lastname: Winterhalter
authors.forename: Mark
authors.initials: M
authors.lastname: Lommel
authors.forename: Thomas
authors.initials: T
authors.lastname: Ruppert
authors.forename: Sabine
authors.initials: S
authors.lastname: Strahl
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D017124:Conserved Sequence; D006031:Glycosylation; D008358:Mannose / Q000378:metabolism*; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D011817:Rabbits; D043323:alpha-Mannosidase / Q000737:chemistry
chemical_list: D015820:Cadherins; C099972:H-cadherin; D010940:Plant Proteins; D043323:alpha-Mannosidase; D008358:Mannose
keywords: CDH13; CID; ConA; EC; ER; GPI; Glycosylation; H-cadherin; LC–MS; Mass spectrometry; O-mannosylation; POMT; T-cad; T-cadherin; XIC; collision-induced dissociation; concanavalin A; endoplasmic reticulum; extracellular cadherin; extracted ion chromatogram; glycosylphosphatidylinositol; liquid chromatography–mass spectrometry; protein O-mannosyltransferase; α-DG; α-dystroglycan