title: Microfabricated device for co-culture of sympathetic neuron and iPS-derived cardiomyocytes.
abstract: Induced pluripotent stem (iPS) cell-derived cardiomyocytes (iPS-CMs) has been expected as a cell source for therapy of serious heart failure. However, it is unclear whether the function of iPS-CMs is modulated by the host sympathetic nervous system. Here we developed a device for co-culture of sympathetic neurons and iPS-CMs using microfabrication technique. The device consisted of a culture chamber and a microelectrode-array (MEA) substrate. The superior cervical ganglion (SCG) neurons were co-cultured with iPS-CMs in a microfabricated device, which had multiple compartments. Several days after seeding, synapses were formed between SCG neurons and iPS-CMs, as confirmed by immunostaining. Spontaneous electrical activities of the SCG neurons and the iPS-CMs were observed from the electrode of the MEA substrate. The beat rate of iPS-CMs increased after electrical stimulation of the co-cultured SCG neurons. Such changes in the beat rate were prevented in the presence of propranolol, a β-adrenoreceptor antagonist. These results suggest that the microfabricated device will be utilized for studying the functional modulation of iPS-CMs by connected sympathetic neurons.
authors.forename: Akimasa
authors.initials: A
authors.lastname: Takeuchi
authors.forename: Kenta
authors.initials: K
authors.lastname: Shimba
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Takayama
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Kotani
authors.forename: Jong-Kook
authors.initials: JK
authors.lastname: Lee
authors.forename: Makoto
authors.initials: M
authors.lastname: Noshiro
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Jimbo
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation; D018920:Coculture Techniques; D004558:Electric Stimulation; D008839:Microelectrodes; D055616:Microtechnology; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D039904:Pluripotent Stem Cells / Q000502:physiology; D011433:Propranolol / Q000494:pharmacology; D017208:Rats, Wistar; D017783:Superior Cervical Ganglion / Q000166:cytology; D013569:Synapses / Q000502:physiology
chemical_list: D000319:Adrenergic beta-Antagonists; D011433:Propranolol