title: Association of pregnancy-associated plasma protein-A gene polymorphism with ischemic stroke in northern Chinese Han population.
abstract: OBJECTIVE
  The pregnancy-associated plasma protein-A (PAPP-A), belonging to the metalloproteinase superfamily, plays an important role in destabilization of atherosclerotic plaques. The aim of this study is to evaluate whether PAPP-A gene polymorphism is associated with the risk of ischemic stroke (IS) in northern Chinese Han populations.
  
  
  METHODS
  A total of 368 patients with IS and 374 aged-matched healthy controls in a Chinese Han population were included in the case-control study. The single-nucleotide polymorphism (SNP) IVS6+95 (rs13290387) in the PAPP-A gene was analyzed by the polymerase chain reaction-ligation detection reaction (PCR-LDR) method.
  
  
  RESULTS
  Compared with the GG+CG genotype, the frequencies of the CC genotype of IVS6+95 (rs13290387) in the patients with IS were significantly higher than those in the controls (P  =  0·026). After adjusting the confounding risk factors of IS (age, gender, smoking, alcohol drinking, hypertension, diabetes mellitus, and hyperlipidemia) by the multivariate logistic analysis, this significant correlation still remained (P  =  0·010).
  
  
  CONCLUSIONS
  The CC genotype of IVS6+95 (rs13290387) was associated with increased incidence of IS in northern Chinese Han populations. This is an initial study to indicate that PAPP-A (rs13290387) might contribute to IS susceptibility in northern Chinese Han populations.
authors.forename: Ruixia
authors.initials: R
authors.lastname: Zhu
authors.forename: Xu
authors.initials: X
authors.lastname: Liu
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: He
mesh_terms: D044466:Asian People / Q000235:genetics; D002545:Brain Ischemia / Q000453:epidemiology / Q000235:genetics*; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011266:Pregnancy-Associated Plasma Protein-A / Q000235:genetics*; D012307:Risk Factors; D020521:Stroke / Q000453:epidemiology / Q000235:genetics*
chemical_list: D011266:Pregnancy-Associated Plasma Protein-A; C579283:PAPPA protein, human
keywords: Genetic polymorphism,; Ischemic stroke,; Pregnancy-associated plasma protein-A