title: Characterization of GM events by insert knowledge adapted re-sequencing approaches.
abstract: Detection methods and data from molecular characterization of genetically modified (GM) events are needed by stakeholders of public risk assessors and regulators. Generally, the molecular characteristics of GM events are incomprehensively revealed by current approaches and biased towards detecting transformation vector derived sequences. GM events are classified based on available knowledge of the sequences of vectors and inserts (insert knowledge). Herein we present three insert knowledge-adapted approaches for characterization GM events (TT51-1 and T1c-19 rice as examples) based on paired-end re-sequencing with the advantages of comprehensiveness, accuracy, and automation. The comprehensive molecular characteristics of two rice events were revealed with additional unintended insertions comparing with the results from PCR and Southern blotting. Comprehensive transgene characterization of TT51-1 and T1c-19 is shown to be independent of a priori knowledge of the insert and vector sequences employing the developed approaches. This provides an opportunity to identify and characterize also unknown GM events.
authors.affiliation: 1] Collaborative Innovation center for biosafety of GMOs, National Center for Molecular Characterization of GMOs, School of Life Science and Biotechnology, Shanghai Jiao Tong University. 800 Dongchuan Road, Shanghai 200240. P. R. China [2].
authors.forename: Litao
authors.initials: L
authors.lastname: Yang
authors.forename: Congmao
authors.initials: C
authors.lastname: Wang
authors.forename: Arne
authors.initials: A
authors.lastname: Holst-Jensen
authors.forename: Dany
authors.initials: D
authors.lastname: Morisset
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Lin
authors.forename: Dabing
authors.initials: D
authors.lastname: Zhang
mesh_terms: D019295:Computational Biology; D003198:Computer Simulation; D018744:DNA, Plant / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D016254:Mutagenesis, Insertional / Q000235:genetics*; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*; D019076:Transgenes / Q000235:genetics*
chemical_list: D018744:DNA, Plant