title: The dynamics of cellular energetics during continuous yeast culture.
abstract: A plethora of data is accumulating from high throughput methods on metabolites, coenzymes, proteins, and nucleic acids and their interactions as well as the signalling and regulatory functions and pathways of the cellular network. The frozen moment viewed in a single discrete time sample requires frequent repetition and updating before any appreciation of the dynamics of component interaction becomes possible. Even then in a sample derived from a cell population, time-averaging of processes and events that occur in out-of-phase individuals blur the detailed complexity of single cell organization. Continuously-grown cultures of yeast can become spontaneously self-synchronized, thereby enabling resolution of far more detailed temporal structure. Continuous on-line monitoring by rapidly responding sensors (O2 electrode and membrane-inlet mass spectrometry for O2, CO2 and H2S; direct fluorimetry for NAD(P)H and flavins) gives dynamic information from time-scales of minutes to hours. Supplemented with capillary electophoresis and gas chromatography mass spectrometry and transcriptomics the predominantly oscillatory behaviour of network components becomes evident, with a 40 min cycle between a phase of increased respiration (oxidative phase) and decreased respiration (reductive phase). Highly pervasive, this ultradian clock provides a coordinating function that links mitochondrial energetics and redox balance to transcriptional regulation, mitochondrial structure and organelle remodelling, DNA duplication and cell division events. Ultimately, this leads to a global partitioning of anabolism and catabolism and the enzymes involved, mediated by a relatively simple ATP feedback loop on chromatin architecture.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Amariei
authors.forename: Rainer
authors.initials: R
authors.lastname: Machné
authors.forename: Kalesh
authors.initials: K
authors.lastname: Sasidharan
authors.forename: Willi
authors.initials: W
authors.lastname: Gottstein
authors.forename: Masaru
authors.initials: M
authors.lastname: Tomita
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Soga
authors.forename: David
authors.initials: D
authors.lastname: Lloyd
authors.forename: Douglas B
authors.initials: DB
authors.lastname: Murray
mesh_terms: D000244:Adenosine Diphosphate / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis; D042002:Chromatin Assembly and Disassembly; D016000:Cluster Analysis; D004247:DNA / Q000378:metabolism; D004138:Dinitrocresols / Q000737:chemistry; D019075:Electrophoresis, Capillary; D004734:Energy Metabolism; D008401:Gas Chromatography-Mass Spectrometry; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism; D009243:NAD / Q000737:chemistry; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000378:metabolism; D059467:Transcriptome
chemical_list: D004138:Dinitrocresols; D017382:Reactive Oxygen Species; D009243:NAD; C024132:4,6-dinitro-o-cresol; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D004247:DNA