title: In situ visualization and detection of protein sulfenylation responses in living cells through a dimedone-based fluorescent probe.
abstract: Sulfenylation is one of the reversible post-translational modifications, playing significant roles in cellular redox homeostasis and signaling systems. Herein, small fluorescent probe (CPD and CPDDM) based live-cell labelling technology for the visualization of protein sulfenylation responses in living cells has been developed. This approach enables the detection of protein sulfenylation without the need for cell lysis, fixation or purification, and permits the noninvasive study of protein sulfenylation in live cells through the direct fluorescent readout. This technology also can realize dynamic tracking of protein sulfenylation in situ with minimal perturbation to sulfenylated proteins and less interference with cellular function. Information on the global distribution and dynamic changes of endogenous protein sulfenylation has been obtained.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China. wpzhu@ecust.edu.cn yfxu@ecust.edu.cn yiyang@ecsut.edu.cn.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yin
authors.forename: Chusen
authors.initials: C
authors.lastname: Huang
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Weiping
authors.initials: W
authors.lastname: Zhu
authors.forename: Yufang
authors.initials: Y
authors.lastname: Xu
authors.forename: Xuhong
authors.initials: X
authors.lastname: Qian
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003512:Cyclohexanones / Q000737:chemistry* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D018922:HL-60 Cells; D022781:Hepatocytes / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D006801:Humans; D015394:Molecular Structure; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013434:Sulfenic Acids / Q000032:analysis* / Q000378:metabolism
chemical_list: D003512:Cyclohexanones; D005456:Fluorescent Dyes; D011506:Proteins; D013434:Sulfenic Acids; C004774:dimedone