title: Quantitative analysis of the effect of triglyceride alkyl-chain length on the partitioning of highly lipophilic compounds to the mesenteric lymph in intestinal cells.
abstract: The purpose of this study was to quantitatively clarify the effect of alky-chain length of a triglyceride in an emulsion on the partitioning of highly lipophilic compounds into the lymph fluid after their oral administration. Highly lipophilic anthraquinone derivatives were orally administered in emulsions to rats. Emulsions composed of long-, medium-, and short-chain triglycerides (LCT, MCT, and SCT emulsions, respectively) were used. The concentrations of the compounds in plasma and lymph fluid were periodically determined and their partitioning to the lymph was calculated using a mathematical model. Intestinal absorption of all compounds was enhanced and the plasma concentrations of the compounds were found to be in the following order: LCT emulsion > MCT emulsion > SCT emulsion. The amounts of each compound recovered in the lymph were not in agreement with their lipophilicity. Quantitative analysis revealed that the partitioning of the compounds to the lymph may be determined by the solubility of the compound in the triglyceride in the form of an emulsion and the amount of triglyceride transferred to the lymph fluid. These results suggest a possibility that the amount of a compound absorbed via the lymph route after oral administration can be quantitatively controlled by the formulations.
authors.affiliation: Division of Pharmaceutics, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka, 569-1094, Japan, iwanaga@gly.oups.ac.jp.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Iwanaga
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kawabata
authors.forename: Makoto
authors.initials: M
authors.lastname: Miyazaki
authors.forename: Masawo
authors.initials: M
authors.lastname: Kakemi
mesh_terms: D000284:Administration, Oral; D000818:Animals; D004655:Emulsions / Q000008:administration & dosage / Q000032:analysis* / Q000737:chemistry; D007408:Intestinal Absorption / Q000187:drug effects / Q000502:physiology*; D008196:Lymph / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008643:Mesentery / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000008:administration & dosage / Q000032:analysis* / Q000737:chemistry
chemical_list: D004655:Emulsions; D014280:Triglycerides