title: Quantification of triacylglycerol molecular species in cocoa butter using high-performance liquid chromatography equipped with nano quantity analyte detector.
abstract: Triacylglycerol (TAG) molecular species were quantified through high-performance liquid chromatography (HPLC) equipped with a nano quantity analyte detector (NQAD). TAG standard compounds, i.e., 1,3-dipalmitoyl-2-oleoylglycerol (β-POP), 1-palmitoyl-2-oleoyl-3-stearoyl-rac-glycerol (β-POS), and 1,3-distearoyl-2-oleoylglycerol (β-SOS), and natural cocoa butter were used for analyses. NQAD gave the first order equation passing through the origin for all TAG standard compounds. TAG molecular species in cocoa butter were quantified using the calibration curves and the obtained values were almost the same as the reported ones of conventional cocoa butter. Furthermore, a recovery test was also carried out and the values were almost 100. Therefore, HPLC-NQAD can be successfully used for the quantification of TAG molecular species in natural fats and oils.
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology.
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Beppu
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Nagai
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Yoshinaga
authors.forename: Hoyo
authors.initials: H
authors.lastname: Mizobe
authors.forename: Koichi
authors.initials: K
authors.lastname: Kojima
authors.forename: Naohiro
authors.initials: N
authors.lastname: Gotoh
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D004041:Dietary Fats / Q000032:analysis*; D005504:Food Analysis / Q000295:instrumentation* / Q000379:methods; D053758:Nanoparticles; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D014280:Triglycerides / Q000032:analysis*
chemical_list: D004041:Dietary Fats; D014280:Triglycerides; C052387:cocoa butter