title: Molecular determinants of the binding specificity of BH3 ligands to BclXL apoptotic repressor.
abstract: B-cell lymphoma extra-large protein (BclXL) serves as an apoptotic repressor by virtue of its ability to recognize and bind to BH3 domains found within a diverse array of proapoptotic regulators. Herein, we investigate the molecular basis of the specificity of the binding of proapoptotic BH3 ligands to BclXL. Our data reveal that while the BH3 ligands harboring the LXXX[A/S]D and [R/Q]XLXXXGD motif bind to BclXL with high affinity in the submicromolar range, those with the LXXXGD motif afford weak interactions. This suggests that the presence of a glycine at the fourth position (G+4)--relative to the N-terminal leucine (L0) within the LXXXGD motif--mitigates binding, unless the LXXXGD motif also contains arginine/glutamine at the -2 position. Of particular note is the observation that the residues at the +4 and -2 positions within the LXXX[A/S]D and [R/Q]XLXXXGD motifs appear to be energetically coupled-replacement of either [A/S]+4 or [R/Q]-2 with other residues has little bearing on the binding affinity of BH3 ligands harboring one of these motifs. Collectively, our study lends new molecular insights into understanding the binding specificity of BH3 ligands to BclXL with important consequences on the design of novel anticancer drugs.
authors.affiliation: Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL, 33136.
authors.forename: Vikas
authors.initials: V
authors.lastname: Bhat
authors.forename: Max B
authors.initials: MB
authors.lastname: Olenick
authors.forename: Brett J
authors.initials: BJ
authors.lastname: Schuchardt
authors.forename: David C
authors.initials: DC
authors.lastname: Mikles
authors.forename: Caleb B
authors.initials: CB
authors.lastname: McDonald
authors.forename: Amjad
authors.initials: A
authors.lastname: Farooq
mesh_terms: D000595:Amino Acid Sequence; D017209:Apoptosis; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions / Q000187:drug effects; D008024:Ligands; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology; D012997:Solvents; D055672:Static Electricity; D013816:Thermodynamics; D051020:bcl-X Protein / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D008024:Ligands; D010455:Peptides; D012997:Solvents; D051020:bcl-X Protein; D012965:Sodium Chloride
keywords: BclXL-BH3 recognition; binding specificity; energetic coupling; salt dependence