title: Nano-anchors with single protein capacity produced with STED lithography.
abstract: Acrylate nanoanchors of subdiffraction-limited diameter are written with optical stimulated emission depletion (STED) lithography. After incubation, 98% of all nanoanchors are loaded quickly with fluorescently labeled antibodies. Controlling the size of the nanoanchors allows for limiting the number of the antibodies. Direct stochastic optical reconstruction microscopy (dSTORM) imaging, statistical distribution of fluorescence, quantitative fluorescence readout, and single molecule blinking consistently prove that 80% of the nanoanchors with a 65 nm diameter are carrying only one antibody each, which are functional as confirmed with live erythrocytes.
authors.affiliation: Johannes Kepler University Linz , 4040 Linz, Austria.
authors.forename: Moritz
authors.initials: M
authors.lastname: Wiesbauer
authors.forename: Richard
authors.initials: R
authors.lastname: Wollhofen
authors.forename: Borislav
authors.initials: B
authors.lastname: Vasic
authors.forename: Kurt
authors.initials: K
authors.lastname: Schilcher
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Jacak
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Klar
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D049329:Nanostructures; D036103:Nanotechnology
chemical_list: D000179:Acrylates; D000906:Antibodies; D005456:Fluorescent Dyes; C036658:acrylic acid