title: Solute removal capacity of high cut-off membrane plasma separators.
abstract: In vitro blood filtration was performed by a closed circuit using high cut-off membrane plasma separators, EVACURE EC-2A10 (EC-2A) and EVACURE EC-4A10 (EC-4A). Samples were obtained from sampling sites before the plasma separator, after each plasma separator, and from the ultrafiltrate of each separator. The sieving coefficient (S.C.) of total protein (TP), albumin (Alb), IgG, interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), fibrinogen (Fib), antithrombin III (AT-III), and coagulation factor XIII (FXIII) were calculated. The S.C. of each solute using EC-2A and EC-A4 were as follows; TP: 0.25 and 0.56, Alb: 0.32 and 0.73, IgG: 0.16 and 0.50, IL-6:0.73 and 0.95, IL-8:0.85 and 0.82, TNF-α: 1.07 and 0.99, Fib: 0 and 0, FXIII: 0.07 and 0.17, respectively. When compared with the conventional type of membrane plasma separators, EVACURE could efficiently remove cytokines while retaining coagulation factors such as fibrinogen. Moreover, EC-2A prevented protein loss, whereas EC-4A could remove approximately 50% of IgG.
authors.affiliation: Medical Engineering Center, Tokyo Medical and Dental University Hospital, Tokyo, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ohkubo
authors.forename: Naoki
authors.initials: N
authors.lastname: Kurashima
authors.forename: Ayako
authors.initials: A
authors.lastname: Nakamura
authors.forename: Satoko
authors.initials: S
authors.lastname: Miyamoto
authors.forename: Soichiro
authors.initials: S
authors.lastname: Iimori
authors.forename: Tatemitsu
authors.initials: T
authors.lastname: Rai
mesh_terms: D001779:Blood Coagulation Factors / Q000378:metabolism; D001781:Blood Component Removal / Q000379:methods*; D016207:Cytokines / Q000097:blood; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D066298:In Vitro Techniques; D008567:Membranes, Artificial; D010949:Plasma / Q000737:chemistry*
chemical_list: D001779:Blood Coagulation Factors; D016207:Cytokines; D007074:Immunoglobulin G; D008567:Membranes, Artificial; D005340:Fibrinogen
keywords: Cytokine; Membrane plasma separator; Plasma exchange therapy; Pore size; Sieving coefficient