title: The effect of manganese-induced cytotoxicity on mRNA expressions of HSP27, HSP40, HSP60, HSP70 and HSP90 in chicken spleen lymphocytes in vitro.
abstract: The purpose of this study was to investigate the effect of manganese (Mn)-induced cytotoxicity on heat shock proteins in chicken spleen lymphocytes. Lymphocytes were cultured in medium in the absence and presence of MnCl2 (2 × 10(-4), 4 × 10(-4), 6 × 10(-4), 8 × 10(-4), 10 × 10(-4), and 12 × 10(-4) mmol/L) for 12, 24, 36, and 48 h in vitro. Then, the mRNA levels of HSP27, HSP40, HSP60, HSP70, and HSP90 were examined by real-time quantitative PCR. The results showed that the mRNA levels of HSP27, HSP40, HSP60, HSP70, and HSP90 in all treatment groups at all time points, except mRNA levels of HSP27 at 48 h, had the same tendency. As manganese concentration increased, the mRNA expression of the heat shock proteins first increased and then decreased. In other words, we demonstrated that the mRNA expression of the heat shock proteins was induced at lower concentrations of manganese and was inhibited at higher concentrations. Mn had a dosage-dependent effect on HSP27, HSP40, HSP60, HSP70, and HSP90 mRNA expression in chicken spleen lymphocytes in vitro.
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Yihao
authors.initials: Y
authors.lastname: Zhu
authors.forename: Xinxing
authors.initials: X
authors.lastname: Lu
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.forename: Shaohe
authors.initials: S
authors.lastname: Cai
authors.forename: Shu
authors.initials: S
authors.lastname: Li
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Teng
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000096:biosynthesis*; D018834:Chaperonin 60 / Q000096:biosynthesis*; D002645:Chickens; D003603:Cytotoxins / Q000009:adverse effects* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D055551:HSP27 Heat-Shock Proteins / Q000096:biosynthesis*; D050956:HSP40 Heat-Shock Proteins / Q000096:biosynthesis*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis*; D008214:Lymphocytes / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008345:Manganese / Q000009:adverse effects* / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis*; D013154:Spleen / Q000378:metabolism* / Q000473:pathology
chemical_list: D030161:Avian Proteins; D018834:Chaperonin 60; D003603:Cytotoxins; D055551:HSP27 Heat-Shock Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D012333:RNA, Messenger; D008345:Manganese