title: [The influence of microcystin-LR on monocytes and lymphocytes of mice].
abstract: OBJECTIVE
  To investigate the influence of microcystin-LR (MC-LR) on monocytes and lymphocytes in blood of mice and to find a sensitive index of toxic effects.
  
  
  METHODS
  Specific pathogen free Kunming male mice, aging 1 month-old,were randomly divided into 5 groups by weights, 7 mice for each group. The mice in 5 groups were exposed to MC-LR through intraperitoneal injection at 0, 3.125,6.250, 12.500 and 25.000 µg/kg respectively for 7 days. Then cytokine levels in the serum were measured by radioimmunoassay, DNA-protein crosslinks (DPC) was measured by the SDS/KCl precipitation technique, and the phagocytosis and ROS of leukocytes were detected by flow cytometry.
  
  
  RESULTS
  The levels of interleukin 6 in the 6.250, 12.500 and 25.000 µg·kg(-1)·d(-1) dose groups were (346.837 ± 25.536), (360.847 ± 37.886) and (434.245 ± 35.858)pg/ml respectively, which were significantly higher than those in the control group which the value was (232.775 ± 32.816) pg/ml (t values were -7.258, -6.760 and -10.966 respectively, P values were all < 0.05).While the level of tumor necrosis factor-alpha was(10.782 ± 0.966) fmol/ml in 25 µg·kg(-1)·d(-1) dose group was statistically lower than it in the control group which the value was (16.878 ± 3.378) fmol/ml (t value was 4.591, P < 0.05). The DPC levels of lymphocytes in 6.250, 12.500 µg·kg(-1)·d(-1) dose group were (242.576 ± 7.545),(241.472 ± 2.793) ng/ml,higher than it in the control group while the value was (228.657 ± 4.130) ng/ml (t value was -4.282, -6.801, P values were all <0.05). The fluorescence intensity of DCF in lymphocytes in the 4 treated groups were separately 3299.37 ± 120.54, 3281.38 ± 58.34, 3308.06 ± 136.12 and 3346.92 ± 108.69, all significantly lower than 3770.81 ± 131.39 in the control group (t values were 6.995, 9.007, 6.472 and 6.577 respectively, and P values were all <0.05). The fluorescence intensity of DCF in monocytes in the 4 treated groups (3271.51 ± 140.79, 3270.05 ± 117.92, 3326.90 ± 114.39 and 3292.49 ± 145.97 respectively) were also significantly lower than the value in the control group was 3841.72 ± 130.92 (t values were 7.847, 8.584, 7.835 and 7.411 respectively, P values were all <0.05). There was no significant difference in other index among the four experiment groups and the control group.
  
  
  CONCLUSIONS
  The MC-LR administered via intraperitoneal injection to mice induced the alterations of some cytokines of monocytes and lymphocytes in blood. By comparison, the ROS of leukocyte was the most sensitive index.
authors.affiliation: Institute of Environmental Medicine, School of Public Health,Tongji Medical College, Huazhong University of Science and Technology, MOE Key Lab of Environment and Health, Wuhan 430030, China.
authors.forename: Xia-ning
authors.initials: XN
authors.lastname: Huang
authors.forename: Yong-lu
authors.initials: YL
authors.lastname: Zhang
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Ye
authors.forename: Wen-qing
authors.initials: WQ
authors.lastname: Xiao
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhong
authors.forename: Kang-ding
authors.initials: KD
authors.lastname: Gu
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D008387:Marine Toxins; D051379:Mice; D008815:Mice, Inbred Strains; D052998:Microcystins / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D016207:Cytokines; D008387:Marine Toxins; D052998:Microcystins; D017382:Reactive Oxygen Species; C057862:cyanoginosin LR