title: Polydopamine as an intermediate layer for silver and hydroxyapatite immobilisation on metallic biomaterials surface.
abstract: Hydroxyapatite (HA) coated implant is more susceptible to bacterial infection as the micro-structure surface which is beneficial for osseointegration, could also become a reservoir for bacterial colonisation. The aim of this study was to introduce the antibacterial effect of silver (Ag) to the biomineralised HA by utilising a polydopamine film as an intermediate layer for Ag and HA immobilisation. Sufficient catechol groups in polydopamine were required to bind chemically stainless steel 316 L, Ag and HA elements. Different amounts of Ag nanoparticles were metallised on the polydopamine grafted stainless steel by varying the immersion time in silver nitrate solution from 12 to 24 h. Another polydopamine layer was then formed on the metallised film, followed by surface biomineralisation in 1.5 Simulated Body Fluid (SBF) solution for 3 days. Several characterisation techniques including X-Ray Photoelectron Spectroscopy, Atomic Force Microscopy, Scanning Electron Microscopy and Contact Angle showed that Ag nanoparticles and HA agglomerations were successfully immobilised on the polydopamine film through an element reduction process. The Ag metallisation at 24 h has killed the viable bacteria with 97.88% of bactericidal ratio. The Ag was ionised up to 7 days which is crucial to prevent bacterial infection during the first stage of implant restoration. The aged functionalised films were considered stable due to less alteration of its chemical composition, surface roughness and wettability properties. The ability of the functionalised film to coat complex and micro scale metal make it suitable for dental and orthopaedic implants application.
authors.affiliation: Medical Implant Technology Group, Faculty of Biosciences & Medical Engineering, Universiti Teknologi Malaysia, 81310 Johor Bahru, Malaysia. Electronic address: syafiqahsaidin@gmail.com.
authors.forename: Syafiqah
authors.initials: S
authors.lastname: Saidin
authors.forename: Pascale
authors.initials: P
authors.lastname: Chevallier
authors.forename: Mohammed Rafiq
authors.initials: MR
authors.lastname: Abdul Kadir
authors.forename: Hendra
authors.initials: H
authors.lastname: Hermawan
authors.forename: Diego
authors.initials: D
authors.lastname: Mantovani
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D007211:Indoles / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D019736:Prostheses and Implants / Q000382:microbiology; D012834:Silver / Q000737:chemistry*; D013193:Stainless Steel / Q000737:chemistry; D013499:Surface Properties; D017155:Wettability
chemical_list: D001672:Biocompatible Materials; D007211:Indoles; D011108:Polymers; C568283:polydopamine; D013193:Stainless Steel; D012834:Silver; D017886:Durapatite
keywords: Antibacterial; Biomineralisation; Hydroxyapatite; Metallisation; Polydopamine; Silver