title: Phosphoproteomic dynamics of chickpea (Cicer arietinum L.) reveals shared and distinct components of dehydration response.
abstract: Reversible protein phosphorylation is a ubiquitous regulatory mechanism that plays critical roles in transducing stress signals to bring about coordinated intracellular responses. To gain better understanding of dehydration response in plants, we have developed a differential phosphoproteome in a food legume, chickpea (Cicer arietinum L.). Three-week-old chickpea seedlings were subjected to progressive dehydration by withdrawing water, and the changes in the phosphorylation status of a large repertoire of proteins were monitored. The proteins were resolved by 2-DE and stained with phosphospecific fluorescent Pro-Q Diamond dye. Mass spectrometric analysis led to the identification of 91 putative phosphoproteins, presumably involved in a variety of functions including cell defense and rescue, photosynthesis and photorespiration, molecular chaperones, and ion transport, among others. Multiple sites of phosphorylation were predicted on several key elements, which include both the regulatory as well as the functional proteins. A critical survey of the phosphorylome revealed a DREPP (developmentally regulated plasma membrane protein) plasma membrane polypeptide family protein, henceforth designated CaDREPP1. The transcripts of CaDREPP1 were found to be differentially regulated under dehydration stress, further corroborating the proteomic results. This work provides new insights into the possible phosphorylation events triggered by the conditions of progressive water-deficit in plants.
authors.affiliation: National Institute of Plant Genome Research , Aruna Asaf Ali Marg, New Delhi 110067, India .
authors.forename: Pratigya
authors.initials: P
authors.lastname: Subba
authors.forename: Pragya
authors.initials: P
authors.lastname: Barua
authors.forename: Rajiv
authors.initials: R
authors.lastname: Kumar
authors.forename: Asis
authors.initials: A
authors.lastname: Datta
authors.forename: Kamlesh Kumar
authors.initials: KK
authors.lastname: Soni
authors.forename: Subhra
authors.initials: S
authors.lastname: Chakraborty
authors.forename: Niranjan
authors.initials: N
authors.lastname: Chakraborty
mesh_terms: D029863:Cicer / Q000235:genetics* / Q000378:metabolism; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D003681:Dehydration / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D053719:Tandem Mass Spectrometry
chemical_list: D017931:DNA Primers; D010750:Phosphoproteins; D010940:Plant Proteins