title: Antitumor effect of 1, 8-cineole against colon cancer.
abstract: Several essential oils possess pharmacological effects. Among the various constituents of essential oils, 1, 8-cineole has been shown to possess pharmacological effects such as anti-bacterial and anti-inflammatory effects. The effect of 1, 8-cineole on human colorectal cancer cells, however, has not reported previously. In this study, we have investigated the anti-proliferative effect of 1, 8-cineole on human colon cancer cell lines HCT116 and RKO by WST-8 and BrdU assays. The cytotoxicity of 1, 8-cineole was investigated by LDH activity and TUNEL staining. The mechanism of apoptosis by 1, 8-cineole was determined by western blot analyses. In in vivo study, RKO cells were injected into the SCID mice and the effect of 1, 8-cineole was investigated. Specific induction of apoptosis, not necrosis, was observed in human colon cancer cell lines HCT116 and RKO by 1, 8-cineole. The treatment with 1, 8-cineole was associated with inactivation of survivin and Akt and activation of p38. These molecules induced cleaved PARP and caspase-3, finally causing apoptosis. In xenotransplanted SCID mice, the 1, 8-cineole group showed significantly inhibited tumor progression compared to the control group. These results indicated 1, 8-cineole suppressed human colorectal cancer proliferation by inducing apoptosis. Based on these studies 1, 8-cineole would be an effective strategy to treat colorectal cancer.
authors.affiliation: Department of Surgery, Teikyo University Chiba Medical Center, Ichihara, Japan.
authors.forename: Soichiro
authors.initials: S
authors.lastname: Murata
authors.forename: Risa
authors.initials: R
authors.lastname: Shiragami
authors.forename: Chihiro
authors.initials: C
authors.lastname: Kosugi
authors.forename: Tohru
authors.initials: T
authors.lastname: Tezuka
authors.forename: Masato
authors.initials: M
authors.lastname: Yamazaki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hirano
authors.forename: Yukino
authors.initials: Y
authors.lastname: Yoshimura
authors.forename: Masato
authors.initials: M
authors.lastname: Suzuki
authors.forename: Kiyohiko
authors.initials: K
authors.lastname: Shuto
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Ohkohchi
authors.forename: Keiji
authors.initials: K
authors.lastname: Koda
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D063646:Carcinogenesis; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D003511:Cyclohexanols / Q000008:administration & dosage* / Q000009:adverse effects; D000077591:Eucalyptol; D045325:HCT116 Cells; D006801:Humans; D051379:Mice; D039821:Monoterpenes / Q000008:administration & dosage* / Q000009:adverse effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D003511:Cyclohexanols; D039821:Monoterpenes; D000077591:Eucalyptol