title: Clerodane diterpenes isolated from Polyalthia longifolia induce apoptosis in human leukemia HL-60 cells.
abstract: Polyalthia is a versatile genus of shrubs and trees found in tropic and sub-tropic regions. In this study, three clerodane diterpenes, kolavenic acid (1), polyalthialdoic acid (2), and 16α-hydroxy-cleroda-3,13(14)Z-dien-15,16-olide (3) isolated from Polyalthia longifolia leaves were evaluated for their apoptotic potential against human leukemia HL-60 cells. Compounds 2 and 3 inhibited cell proliferation with IC₅₀ values of 21.8 and 13.7 μM, respectively. Morphological changes and DNA fragmentation analysis indicated that these diterpenes induce apoptotic cell death in the HL-60 cells. Our results revealed the importance of P. longifolia as a chemopreventive medicinal plant.
authors.affiliation: Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Andalas.
authors.forename: Dina Permata
authors.initials: DP
authors.lastname: Sari
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ninomiya
authors.forename: Mai
authors.initials: M
authors.lastname: Efdi
authors.forename: Adlis
authors.initials: A
authors.lastname: Santoni
authors.forename: Sanusi
authors.initials: S
authors.lastname: Ibrahim
authors.forename: Kaori
authors.initials: K
authors.lastname: Tanaka
authors.forename: Mamoru
authors.initials: M
authors.lastname: Koketsu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D045785:Diterpenes, Clerodane / Q000302:isolation & purification* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells; D006801:Humans; D018515:Plant Leaves / Q000737:chemistry; D031044:Polyalthia / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D045785:Diterpenes, Clerodane