title: N-acyl-homoserine lactone uptake and systemic transport in barley rest upon active parts of the plant.
abstract: Bacteria communicate with each other in a population density-dependent process known as quorum sensing. N-acyl-homoserine lactones (HSLs) are the autoinducers of Gram-negative bacteria and the best-studied quorum sensing signals so far. HSLs induce various responses in plants, including systemic resistance and root development. Here, we used different methods, including tritium labelling, sensor strain assays and monoclonal antibodies (mAbs), to analyse the uptake and translocation of C8- and C10- homoserine lactones into barley (Hordeum vulgare cv Barke). Both HSLs were already systemically transported into the shoot at 2 h after application. HSL uptake could be inhibited by orthovanadate, demonstrating that ABC transporters are involved in the uptake. Root transport occurs predominantly via the central cylinder, which was shown by transport inhibition via KCl application and autoradiography of root cross-sections. Furthermore, a newly established detection method with mAbs allowed the first detection of a systemic transport of long-chain HSLs in plants. The coupled use of different HSL detection methods demonstrated that the uptake and transport of HSLs into barley does not occur passively, but relies, at least partially, on active processes in the plant.
authors.affiliation: Helmholtz Zentrum München, German Research Centre for Environmental Health, GmbH Department Microbe-Plant Interactions, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany.
authors.forename: Tina
authors.initials: T
authors.lastname: Sieper
authors.affiliation: Academy of Sciences of the Czech Republic, Institute of Experimental Botany, Videnska 1083, 14220, Prague 4, Czech Republic.
authors.forename: Sandor
authors.initials: S
authors.lastname: Forczek
authors.affiliation: Academy of Sciences of the Czech Republic, Institute of Experimental Botany, Videnska 1083, 14220, Prague 4, Czech Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Matucha
authors.affiliation: Helmholtz Zentrum München, German Research Centre for Environmental Health, GmbH Department Microbe-Plant Interactions, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Krämer
authors.affiliation: Helmholtz Zentrum München, German Research Centre for Environmental Health, GmbH Department Microbe-Plant Interactions, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany.
authors.forename: Anton
authors.initials: A
authors.lastname: Hartmann
authors.affiliation: Helmholtz Zentrum München, German Research Centre for Environmental Health, GmbH Department Microbe-Plant Interactions, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schröder
mesh_terms: D054742:Acyl-Butyrolactones; D001692:Biological Transport; D001467:Hordeum / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D053038:Quorum Sensing
chemical_list: D054742:Acyl-Butyrolactones
keywords: N-acyl-homoserine lactones (HSLs); barley (Hordeum vulgare); monoclonal antibodies; quorum sensing; signalling