title: Influence of cataract maturity on aqueous humor lipid peroxidation markers and antioxidant enzymes.
abstract: OBJECTIVE
  The impact of cataract maturity on the aqueous humor (AH) oxidant/antioxidant balance is largely controversial. This study was aimed at assessing the relationships between cataract maturity and AH lipid peroxidation markers and enzymatic antioxidants.
  
  
  METHODS
  The concentrations of conjugated dienes (CD), lipofuscin-like fluorescent end-products (LLF), soluble proteins, as well as the activities of superoxide dismutase (SOD) and catalase (CAT) were measured in AH samples from nondiabetic patients with either immature (n=15) or mature (n=15) cataract.
  
  
  RESULTS
  In the overall AH sample, the mean values of CD, LLF, SOD, and CAT were 0.160 ± 0.024 (OD234), 166 ± 27 RFU, 24.5 ± 7.1 U/ml, and 31.9 ± 3.9 pmol/ml, respectively. CD was positively correlated with SOD (r=0.647; P<0.001), CAT (r=-0.394; P=0.031), and LLF (r=-0.399; P=0.029). The LLF was negatively correlated with SOD (r=-0.461; P=0.010). In samples adjusted for confounding factors, differences between immature and mature cataract groups regarding SOD, CD, LLF, and total proteins were significant (P<0.05; for all variables). The multiple logistic regression analysis identified LLF (OR=4.08; P=0.038) and SOD (OR=4.99; P=0.031) as independent predictors of cataract maturity.
  
  
  CONCLUSIONS
  These results suggest that AH lipid peroxidation markers and antioxidants may significantly depend on the cataract maturity stage.
authors.affiliation: Institute of Biochemistry, Medical Faculty Pristina, Kosovska Mitrovica, Serbia.
authors.forename: D J
authors.initials: DJ
authors.lastname: Miric
authors.affiliation: Institute of Biochemistry, Medical Faculty Pristina, Kosovska Mitrovica, Serbia.
authors.forename: B M
authors.initials: BM
authors.lastname: Kisic
authors.affiliation: Eye Disease Clinic, Medical Faculty Pristina, Kosovska Mitrovica, Serbia.
authors.forename: L D
authors.initials: LD
authors.lastname: Zoric
authors.affiliation: Department of Informatics, State University of Novi Pazar, Novi Pazar, Serbia.
authors.forename: B M
authors.initials: BM
authors.lastname: Miric
authors.affiliation: Eye Disease Clinic, Medical Faculty Pristina, Kosovska Mitrovica, Serbia.
authors.forename: M
authors.initials: M
authors.lastname: Mirkovic
authors.affiliation: Institute of Pharmacology, Medical Faculty Pristina, Kosovska Mitrovica, Serbia.
authors.forename: R
authors.initials: R
authors.lastname: Mitic
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology; D000975:Antioxidants / Q000378:metabolism; D001082:Aqueous Humor / Q000201:enzymology*; D015415:Biomarkers / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism*; D002386:Cataract / Q000473:pathology*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000378:metabolism*; D008062:Lipofuscin / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D008054:Lipid Peroxides; D008062:Lipofuscin; C577219:CAT protein, human; D002374:Catalase; D013482:Superoxide Dismutase