title: A validation study of a multiplex INDEL assay for forensic use in four Chinese populations.
abstract: Insertion/deletion (INDELs) marker sets can serve as a useful supplementary tool for human identification. A commercial kit, the Qiagen DIPplex(®) Investigator kit, multiplexes 30 biallelic autosomal INDELs and Amelogenin for forensic use. We performed a validation study based on the DIPplex(®) kit in four Chinese populations: Han, Tibetan, Uyghur, and Kazakh. There were no significant departures from Hardy-Weinberg equilibrium or significant linkage disequilibrium (pair-wised r(2)<0.2) between the 30 INDELs. The random match probabilities were in the range of 3.84 × 10(-11) to 1.20 × 10(-12), and the power of exclusion was >0.99. The multiplex PCR was optimized for a 5-μL volume, full profiles were obtained with 0.062 ng/μL of template DNA, and excellent performance was obtained with degraded casework samples. This study demonstrates that the multiplex INDEL assay can be used as a supplementary method for degraded DNA detection in the studied Chinese populations.
authors.affiliation: Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, PR China; Key Laboratory of Medical Epigenetics, Tianjin Research Center of Basic Medical Sciences, Tianjin 300070, PR China.
authors.forename: Yi-Liang
authors.initials: YL
authors.lastname: Wei
authors.affiliation: Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, PR China; Department of Forensic Science, Chinese People's Public Security University, Beijing 100038, PR China.
authors.forename: Cui-Jiao
authors.initials: CJ
authors.lastname: Qin
authors.affiliation: Department of Forensic Science, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Dong
authors.affiliation: Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, PR China; Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Jia
authors.affiliation: Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, PR China. Electronic address: licaixia@tsinghua.org.cn.
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Li
mesh_terms: D002681:China; D053836:DNA Degradation, Necrotic; D016172:DNA Fingerprinting; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D005828:Genetics, Population; D006801:Humans; D054643:INDEL Mutation; D008297:Male; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
keywords: F(st); Heterozygosity; INDEL; Individual identification; Population genetics