title: A novel peptide specifically targeting ovarian cancer identified by in vivo phage display.
abstract: Discovery of peptide ligands that can target human ovarian cancer and deliver chemotherapeutics offers new opportunity for cancer therapy. The advent of phage-displayed peptide library facilitated the screening of such peptides. In vivo screening that set in a microanatomic and functional context was applied in our study, and a novel peptide WSGPGVWGASVK targeting ovarian cancer was isolated. The phage clone PC3-1 displaying peptide WSGPGVWGASVK can gain effective access to accumulate in the tumor sites after intravenous injection while reducing its accumulation in normal organs. Positive immunostaining of PC3-1 was located in both sites of tumor cells and tumor blood vessels, which resulted in a diffuse binding pattern through the tumor. In vitro study results confirmed the capability of peptide WSGPGVWGASVK binding to and being internalized by both tumor cells and angiogenic endothelial cells. Flow cytometry analysis revealed that the peptide bound to SKOV3 cells with Kd value of 5.43 ± 0.4 μM. Taken together, it suggested that peptide WSGPGVWGASVK is a lead candidate for delivering therapeutics to penetrate into tumors.
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu, 610065, China.
authors.forename: Chuying
authors.initials: C
authors.lastname: Ma
authors.forename: Guangfu
authors.initials: G
authors.lastname: Yin
authors.forename: Danhong
authors.initials: D
authors.lastname: Yan
authors.forename: Xueling
authors.initials: X
authors.lastname: He
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wei
authors.forename: Zhongbing
authors.initials: Z
authors.lastname: Huang
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D051379:Mice; D009368:Neoplasm Transplantation; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism / Q000493:pharmacokinetics; D010051:Ovarian Neoplasms / Q000188:drug therapy*; D011485:Protein Binding; D014018:Tissue Distribution
chemical_list: D009842:Oligopeptides
keywords: cell adhesion; in vivo screening; ovarian cancer; peptide ligands; phage display