title: Organ-specific function of adhesion G protein-coupled receptor GPR126 is domain-dependent.
abstract: Despite their abundance and multiple functions in a variety of organ systems, the function and signaling mechanisms of adhesion G protein-coupled receptors (GPCRs) are poorly understood. Adhesion GPCRs possess large N termini containing various functional domains. In addition, many of them are autoproteolytically cleaved at their GPS sites into an N-terminal fragment (NTF) and C-terminal fragment. Here we demonstrate that Gpr126 is expressed in the endocardium during early mouse heart development. Gpr126 knockout in mice and knockdown in zebrafish caused hypotrabeculation and affected mitochondrial function. Ectopic expression of Gpr126-NTF that lacks the GPS motif (NTF(ΔGPS)) in zebrafish rescued the trabeculation but not the previously described myelination phenotype in the peripheral nervous system. These data support a model in which the NTF of Gpr126, in contrast to the C-terminal fragment, plays an important role in heart development. Collectively, our analysis provides a unique example of the versatile function and signaling properties of adhesion GPCRs in vertebrates.
authors.affiliation: Department of Cardiac Development and Remodelling, Max Planck Institute for Heart and Lung Research, 61231 Bad Nauheim, Germany.
authors.forename: Chinmoy
authors.initials: C
authors.lastname: Patra
authors.forename: Machteld J
authors.initials: MJ
authors.lastname: van Amerongen
authors.forename: Subhajit
authors.initials: S
authors.lastname: Ghosh
authors.forename: Filomena
authors.initials: F
authors.lastname: Ricciardi
authors.forename: Amna
authors.initials: A
authors.lastname: Sajjad
authors.forename: Tatyana
authors.initials: T
authors.lastname: Novoyatleva
authors.forename: Amit
authors.initials: A
authors.lastname: Mogha
authors.forename: Kelly R
authors.initials: KR
authors.lastname: Monk
authors.forename: Christian
authors.initials: C
authors.lastname: Mühlfeld
authors.forename: Felix B
authors.initials: FB
authors.lastname: Engel
mesh_terms: D000818:Animals; D004699:Endocardium / Q000166:cytology / Q000196:embryology*; D051379:Mice; D018345:Mice, Knockout; D008929:Mitochondria, Heart / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D009928:Organ Specificity / Q000502:physiology; D017434:Protein Structure, Tertiary; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C550767:Gpr126 protein, mouse; D043562:Receptors, G-Protein-Coupled; D029961:Zebrafish Proteins; C543742:stx7l protein, zebrafish