title: FTIR study of protective action of deferoxamine and deferiprone on the kidney tissues of aluminum loaded mice.
abstract: The present study was designed to evaluate the FTIR spectra of the aluminum exposed kidney tissues and recovered by chelating agents DFO and DFP then showed significant alteration on the major biochemical constituents such as lipids, proteins and glycogen at molecular level. The significant increased in the peak area of glycogen from 0.006±0.001 to 0.187±0.032 may be the interruption of aluminum in the calcium metabolism and the reduced level of calcium. The peak area value of amide A significantly decreased from control (4.931±1.446) to aluminum (1.234±0.052), but improved by DFP and DFO+DFP from 2.658±0.153 to 3.252±0.070 respectively. Amide I and amide II peak area values also decreased from 1.690±0.133 to 0.811±0.192 and 1.158±0.050 to 0.489±0.047 but treated with DFP and DFO+DFP significantly improved. This result suggests an alteration in the protein profile. The absence of Olefinic=CH stretching band, C=O stretching of triglycerides and ring breathing mode in the DNA bases in aluminum exposure kidney suggests an altered lipid levels. Treated with DFP and DFO+DFP mice were considerably increased in lipid peroxidative markers. Further, assessed the activities of enzymatic antioxidants and measured the levels of nonenzymatic antioxidants. Concentrations of trace elements were found by ICP-OES. Histopathology of chelating agents treated kidney showed reduced renal damage in aluminum induced mice. Thus, histopathological findings confirmed the biochemical observations of this study. This results demonstrated that FTIR spectroscopy can be successfully applied to toxicological and biotoxicology studies.
authors.affiliation: Department of Physics, Annamalai University, Annamalai Nagar, Tamilnadu 608002, India. Electronic address: girihari777@yahoo.com.
authors.forename: S
authors.initials: S
authors.lastname: Sivakumar
authors.forename: Chandra Prasad
authors.initials: CP
authors.lastname: Khatiwada
authors.forename: J
authors.initials: J
authors.lastname: Sivasubramanian
authors.forename: B
authors.initials: B
authors.lastname: Raja
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D000818:Animals; D002614:Chelating Agents / Q000494:pharmacology*; D000077543:Deferiprone; D003676:Deferoxamine / Q000494:pharmacology*; D007668:Kidney / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology*; D008297:Male; D051379:Mice; D020011:Protective Agents / Q000494:pharmacology*; D011728:Pyridones / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D002614:Chelating Agents; D020011:Protective Agents; D011728:Pyridones; D000077543:Deferiprone; D000535:Aluminum; D003676:Deferoxamine
keywords: Aluminum; DFO and DFP; FTIR; ICP-OES; Kidney