title: Decoration of electrospun nanofibers with monomeric catechols to facilitate cell adhesion.
abstract: Monomeric catechols are displayed on the surface of polymeric nanofibers by robust catechol-thiol interactions to enhance cell adhesion and migration. Dihydroxyphenyl propionic acid is chemically conjugated to primary amine groups of poly(ϵ-caprolactone)-poly(ethylene glycol)-amine (PCL-PEG) nanofibers to display catechol moieties on the surface. At basic pH, catecholized nanofibers incorporate thiol groups at a five-fold higher rate than at acidic pH, while catechol-coated surfaces do not show any pH-dependent binding. Live/dead cell staining indicates that the catecholized nanofibers do not exert any cytotoxic effects. Also, NIH 3T3 cells cultured on the catecholized nanofibers show increased attachment and migration that is proportional to the amount of the immobilized catechol moieties on the surface. These results clearly indicate that 6 nmol of monomeric catechols on the surface of nanofiber can promote cell adhesion and migration by thiol-catehol interactions.
authors.affiliation: Department of Biomedical Materials Engineering, Kangwon National University, Chuncheon, 200-701, Republic of Korea; Institute of Bioscience and Bioengineering, Kangwon National University, Republic of Korea.
authors.forename: Ji Suk
authors.initials: JS
authors.lastname: Choi
authors.forename: Phillip B
authors.initials: PB
authors.lastname: Messersmith
authors.forename: Hyuk Sang
authors.initials: HS
authors.lastname: Yoo
mesh_terms: D000818:Animals; D002396:Catechols / Q000737:chemistry*; D002448:Cell Adhesion; D002465:Cell Movement; D006863:Hydrogen-Ion Concentration; D051379:Mice; D041681:NIH 3T3 Cells; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D013499:Surface Properties
chemical_list: D002396:Catechols; D011091:Polyesters; C016240:polycaprolactone; D011092:Polyethylene Glycols; C034221:catechol
keywords: catechol; cell adhesion; cell migration; chemical surface immobilization; nanofibers