title: Molecular characterization of a novel botulinum neurotoxin type H gene.
abstract: We sequenced the 2 botulinum toxin gene clusters of Clostridium botulinum strain IBCA10-7060 type Bh. The sequence of bont/H differed substantially from the sequences of the 7 known bont genes for toxin types A-G. The 5' one-third terminus of bont/H that codes for the botulinum toxin light chain differed markedly from the light chain coding sequences of toxin types A-G. The 3' two-thirds terminus of bont/H that codes for the botulinum toxin heavy chain contained a novel Hn translocation domain coding sequence and a nonneutralizing type A-like Hc binding domain coding sequence. bont/H was part of an orfX toxin gene cluster that was located at a unique chromosomal site distant from those used by other botulinum toxin gene clusters. The bont/B sequence was similar to that of subtype bont/B2 and was located within its ha toxin gene cluster at the oppA/brnQ site. Our findings further establish that C. botulinum IBCA10-7060 produces novel BoNT/H.
authors.affiliation: Infant Botulism Treatment and Prevention Program, California Department of Public Health, Richmond, California.
authors.forename: Nir
authors.initials: N
authors.lastname: Dover
authors.forename: Jason R
authors.initials: JR
authors.lastname: Barash
authors.forename: Karen K
authors.initials: KK
authors.lastname: Hill
authors.forename: Gary
authors.initials: G
authors.lastname: Xie
authors.forename: Stephen S
authors.initials: SS
authors.lastname: Arnon
mesh_terms: D001905:Botulinum Toxins / Q000235:genetics* / Q000378:metabolism; D001906:Botulism / Q000382:microbiology; D003014:Clostridium botulinum / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D023061:Gene Order; D006801:Humans; D007223:Infant; D005810:Multigene Family; D010802:Phylogeny; D021122:Protein Subunits / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004269:DNA, Bacterial; D021122:Protein Subunits; D001905:Botulinum Toxins
keywords: Clostridium botulinum; botulinum toxin; botulinum toxin type H; botulism