title: Whole organ cross-section chemical imaging using label-free mega-mosaic FTIR microscopy.
abstract: FTIR chemical imaging has been demonstrated as a promising technique to construct automated systems to complement histopathological evaluation of biomedical tissue samples. The rapid chemical imaging of large areas of tissue has previously been a limiting factor in this application. Consequently, smaller areas of tissue have previously had to be sampled, possibly introducing sampling bias and potentially missing diagnostically important areas. In this report a high spatial resolution chemical image of a whole prostate cross section is shown comprising 66 million pixels. Each pixel represents an area 5.5 × 5.5 μm(2) of tissue and contains a full infrared spectrum providing a chemical fingerprint. The data acquisition time was 14 hours, thus showing that a clinical time frame of hours rather than days has been achieved.
authors.affiliation: Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK. peter.gardner@manchester.ac.uk.
authors.forename: Paul
authors.initials: P
authors.lastname: Bassan
authors.forename: Ashwin
authors.initials: A
authors.lastname: Sachdeva
authors.forename: Jonathan H
authors.initials: JH
authors.lastname: Shanks
authors.forename: Mick D
authors.initials: MD
authors.lastname: Brown
authors.forename: Noel W
authors.initials: NW
authors.lastname: Clarke
authors.forename: Peter
authors.initials: P
authors.lastname: Gardner
mesh_terms: D008856:Microscopy, Fluorescence / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*