title: Validation of high-throughput genotoxicity assay screening using γH2AX in-cell western assay on HepG2 cells.
abstract: In vitro genotoxicity tests used in regulatory toxicology studies are sensitive, but the occurrence of irrelevant positive results is high compared with carcinogenicity studies in rodents. Current in vitro genotoxicity tests are also often limited by relatively low throughput. The aim of this study was to validate an in vitro genotoxic assay in a 96-well plate format that allows the simultaneous examination of cytotoxicity and genotoxicity. The test is based on the quantification of the phosphorylation of the histone H2AX (γH2AX), which reflects a global genotoxic insult, using the In-Cell Western technique. The assay was evaluated on HepG2 cells by testing a list of 61 compounds recommended by the European Center for the Validation of Alternative Methods (ECVAM), whose genotoxic potential has already been characterized. The γH2AX assay on HepG2 cell line was highly sensitive: 75% of the genotoxic compounds gave a positive result, and specific: 90-100% of nongenotoxic compounds gave negative results. Compared with the micronucleus genotoxicity assay using the same cell line and test compounds, the γH2AX assay was more sensitive and specific. In sum, the high-throughput γH2AX assay described here can accurately detect simultaneously the genotoxic and the cytotoxic potential of compounds with different modes of mutagenic action, notably those who required metabolic activation. The use of this assay in the early discovery phase of drug development may prove to be a valuable way to assess the genotoxic potential of xenobiotics.
authors.affiliation: INRA, UMR1331, Toxalim, Research Centre in Food Toxicology, F-31027, Toulouse, France; Université de Toulouse, INPT, UPS, UMR1331, F-31062, Toulouse, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Khoury
authors.forename: Daniel
authors.initials: D
authors.lastname: Zalko
authors.forename: Marc
authors.initials: M
authors.lastname: Audebert
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D001711:Biotransformation / Q000187:drug effects; D015153:Blotting, Western; D004249:DNA Damage / Q000187:drug effects*; D056945:Hep G2 Cells; D057166:High-Throughput Screening Assays; D006657:Histones / Q000378:metabolism*; D006801:Humans; D015162:Micronucleus Tests / Q000379:methods*; D009152:Mutagenicity Tests / Q000379:methods*; D010766:Phosphorylation / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; C468783:H2AX protein, human; D006657:Histones
keywords: Ames assay; ECVAM; H2AX; HepG2; genotoxicity; micronucleus