title: Enriched glucose and dextrin mannitol-based media modulates fibroblast behavior on bacterial cellulose membranes.
abstract: Bacterial cellulose (BC) produced by Gluconacetobacter hansenii is a suitable biopolymer for biomedical applications. In order to modulate the properties of BC and expand its use as substrate for tissue engineering mainly in the form of biomembranes, glucose or dextrin were added into a BC fermentation mannitol-based medium (BCGl and BCDe, respectively) under static culture conditions. SEM images showed effects on fiber density and porosity on both sides of the BC membranes. Both enriched media decreased the BET surface area, water holding capacity, and rehydration rate. Fourier transform infrared (attenuated total reflectance mode) spectroscopy (FTIR-ATR) analysis revealed no change in the chemical structure of BC. L929 fibroblast cells were seeded on all BC-based membranes and evaluated in aspects of cell adhesion, proliferation and morphology. BCG1 membranes showed the highest biological performance and hold promise for the use in tissue engineering applications.
authors.affiliation: Integrated Technologies Laboratory, Department of Chemical and Food Engineering, Brazil.
authors.forename: Taisa R
authors.initials: TR
authors.lastname: Stumpf
authors.forename: Renata A N
authors.initials: RA
authors.lastname: Pértile
authors.forename: Carlos R
authors.initials: CR
authors.lastname: Rambo
authors.forename: Luismar M
authors.initials: LM
authors.lastname: Porto
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology; D003912:Dextrins / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology; D041821:Gluconacetobacter / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D005947:Glucose / Q000737:chemistry*; D006801:Humans; D008353:Mannitol / Q000737:chemistry*; D008567:Membranes, Artificial; D016062:Porosity
chemical_list: D001672:Biocompatible Materials; D003470:Culture Media; D003912:Dextrins; D008567:Membranes, Artificial; D008353:Mannitol; D002482:Cellulose; D005947:Glucose
keywords: Bacterial cellulose; Biocompatibility; Dextrin; Enriched media for fibroblasts; Glucose