title: Characterization of nitrifying microbial community in a submerged membrane bioreactor at short solids retention times.
abstract: This study investigated the nitrifying bacterial community in membrane bioreactor (MBR) at short solids retention times (SRTs) of 3, 5 and 10 days. The denaturing gradient gel electrophoresis results showed that different types of ammonia-oxidizing bacteria (AOB) can survive at different operating conditions. The diversity of AOB increased as the SRT increased. The real-time PCR results showed that the amoA gene concentrations were similar when MBRs were stabilized, and it can be a good indicator of stabilized nitrification. The results of clone library indicated that Nitrosomonas was the dominant group of AOB in three reactors. The microarray results showed that Nitrospira was the dominant group of nitrite-oxidizing bacteria (NOB) in the system. All groups of AOB and NOB except Nitrosolobus and Nitrococcus were found in MBR, indicated that the nitrifying bacterial community structure was more complicated. The combination of some molecular tools can provide more information of microbial communities.
authors.affiliation: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, PR China; Department of Civil and Environmental Engineering, University of California, Berkeley, CA 94720-1710, USA.
authors.forename: Liang
authors.initials: L
authors.lastname: Duan
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Song
authors.forename: Siqing
authors.initials: S
authors.lastname: Xia
authors.forename: Slawomir W
authors.initials: SW
authors.lastname: Hermanowicz
mesh_terms: D000641:Ammonia / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D019149:Bioreactors / Q000382:microbiology*; D058645:Denaturing Gradient Gel Electrophoresis; D005453:Fluorescence; D018628:Gene Dosage; D015964:Gene Expression Regulation, Bacterial; D015723:Gene Library; D005798:Genes, Bacterial / Q000235:genetics; D008567:Membranes, Artificial; D058465:Nitrification; D020411:Oligonucleotide Array Sequence Analysis; D010084:Oxidation-Reduction; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D008567:Membranes, Artificial; D000641:Ammonia
keywords: Ammonia-oxidizing bacteria (AOB); Membrane bioreactor; Nitrification; Nitrite-oxidizing bacteria (NOB); Short solids retention time (SRT)