title: A synthetic peptide-acrylate surface for production of insulin-producing cells from human embryonic stem cells.
abstract: Human embryonic stem cells (hESCs), due to their self-renewal capacity and pluripotency, have become a potential source of transplantable β-cells for the treatment of diabetes. However, it is imperative that the derived cells fulfill the criteria for clinical treatment. In this study, we replaced common Matrigel with a synthetic peptide-acrylate surface (Synthemax) to expand undifferentiated hESCs and direct their differentiation in a defined and serum-free medium. We confirmed that the cells still expressed pluripotent markers, had the ability to differentiate into three germ layers, and maintained a normal karyotype after 10 passages of subculture. Next, we reported an efficient protocol for deriving nearly 86% definitive endoderm cells from hESCs under serum-free conditions. Moreover, we were able to obtain insulin-producing cells within 21 days following a simple three-step protocol. The results of immunocytochemical and quantitative gene expression analysis showed that the efficiency of induction was not significantly different between the Synthemax surface and the Matrigel-coated surface. Thus, we provided a totally defined condition from hESC culture to insulin-producing cell differentiation, and the derived cells could be a therapeutic resource for diabetic patients in the future.
authors.affiliation: 1 Bioresource Collection and Research Center, Food Industry Research and Development Institute , Hsinchu, Taiwan .
authors.forename: Pei-Yi
authors.initials: PY
authors.lastname: Lin
authors.forename: Shih-Han
authors.initials: SH
authors.lastname: Hung
authors.forename: Yao-Chen
authors.initials: YC
authors.lastname: Yang
authors.forename: Li-Chuan
authors.initials: LC
authors.lastname: Liao
authors.forename: Yi-Cheng
authors.initials: YC
authors.lastname: Hsieh
authors.forename: Hsan-Jan
authors.initials: HJ
authors.lastname: Yen
authors.forename: Huai-En
authors.initials: HE
authors.lastname: Lu
authors.forename: Maw-Sheng
authors.initials: MS
authors.lastname: Lee
authors.forename: I-Ming
authors.initials: IM
authors.lastname: Chu
authors.forename: Shiaw-Min
authors.initials: SM
authors.lastname: Hwang
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry; D004338:Drug Combinations; D053595:Embryonic Stem Cells / Q000502:physiology*; D006801:Humans; D007328:Insulin / Q000096:biosynthesis*; D007797:Laminin / Q000737:chemistry; D018716:Molecular Mimicry; D010446:Peptide Fragments / Q000737:chemistry*; D011509:Proteoglycans / Q000737:chemistry; D013499:Surface Properties; D019096:Vitronectin / Q000737:chemistry
chemical_list: D000179:Acrylates; D001672:Biocompatible Materials; D003470:Culture Media; D004338:Drug Combinations; D007328:Insulin; D007797:Laminin; D010446:Peptide Fragments; D011509:Proteoglycans; D019096:Vitronectin; C059485:matrigel; D003094:Collagen