title: Antioxidant status in oral subchronic toxicity of fipronil and fluoride co-exposure in buffalo calves.
abstract: The effects of fipronil and fluoride co-exposure were investigated on antioxidant status of buffalo calves. A total of 24 healthy male buffalo calves divided into 4 groups were treated for 98 consecutive days. Group I, receiving no treatment, served as the control. Animals of groups II and III were orally administered with fipronil at the dosage of 0.5 mg/kg/day and sodium fluoride (NaF) at the dosage of 6.67 mg/kg/day, respectively, for 98 days. Group IV was coadministered with fipronil and NaF at the same dosages as groups II and III. Administration of fipronil alone produced significant elevation in lipid peroxidation (LPO) and decrease in the levels of nonenzymatic antioxidant glutathione (GSH). However, it did not produce any significant effect on the activities of enzymatic antioxidants including glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD). NaF exposure led to enhanced oxidative stress as shown by significant increase in the LPO and SOD activities while GPx and CAT activities and GSH levels were significantly decreased. Co-exposure to fipronil and NaF showed additive effects on LPO, GPx activity, and GSH levels.
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India.
authors.forename: Kamalpreet Kaur
authors.initials: KK
authors.lastname: Gill
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India vkdumka@yahoo.com.
authors.forename: Vinod Kumar
authors.initials: VK
authors.lastname: Dumka
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000097:blood; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D005459:Fluorides / Q000008:administration & dosage / Q000633:toxicity*; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects; D011720:Pyrazoles / Q000008:administration & dosage / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000097:blood; D059550:Toxicity Tests, Subchronic
chemical_list: D000975:Antioxidants; D011720:Pyrazoles; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D005459:Fluorides; C082360:fipronil
keywords: Fipronil; buffalo calves; co-exposure; oxidative stress; sodium fluoride