title: Snapshot of a DNA polymerase while incorporating two consecutive C5-modified nucleotides.
abstract: Functional nucleotides are important in many cutting-edge biomolecular techniques. Often several modified nucleotides have to be incorporated consecutively. This structural study of KlenTaq DNA polymerase, a truncated form of Thermus aquaticus DNA polymerase, gives first insights how multiple modifications are processed by a DNA polymerase and, therefore, contribute to the understanding of these enzymes in their interplay with artificial substrates.
authors.affiliation: Departments of Chemistry and Biology, Konstanz Research School Chemical Biology, University of Konstanz , Universitätsstrasse 10, 78457 Konstanz, Germany.
authors.forename: Samra
authors.initials: S
authors.lastname: Obeid
authors.forename: Holger
authors.initials: H
authors.lastname: Bußkamp
authors.forename: Wolfram
authors.initials: W
authors.lastname: Welte
authors.forename: Kay
authors.initials: K
authors.lastname: Diederichs
authors.forename: Andreas
authors.initials: A
authors.lastname: Marx
mesh_terms: D008958:Models, Molecular; D015394:Molecular Structure; D009711:Nucleotides / Q000737:chemistry* / Q000378:metabolism; D019914:Taq Polymerase / Q000737:chemistry* / Q000378:metabolism; D013824:Thermus / Q000201:enzymology*
chemical_list: D009711:Nucleotides; D019914:Taq Polymerase