title: Regulatory T cells in cutaneous lesions of patients with Paracoccidioidomycosis.
abstract: Paracoccidioidomycosis (PCM) is a systemic mycosis caused by the dimorphic fungus Paracoccidioides brasiliensis, with high incidence in Brazil and very significant in Latin America. The disease is clinically classified as acute, subacute or chronic where the primary lesion initiates in the lungs and can spread to other organs such as the skin and mucous membranes. The lesions are characterized by granulomatous formation, organized according to the type of pattern of host immune response. We demonstrated and quantified by immunohistochemistry the expression of Foxp3, CD25, TGF-beta and IL-10 in thirty cutaneous lesions with different presentation of granulomatous response. Cells expressing Foxp3 and CD25 were increased in lesions with compact granulomas. The expression of TGF-beta and IL-10 was similar in all PCM lesions. As previous studies, our data suggest the correlation of Treg cells with the chronicity of the disease and the participation in suppressing mechanism as a possible source of IL-10. TGF-β and IL-10, two important suppressor cytokines, are expressed in great amounts in the lesions but our results do not allow correlating with the differences in the granulomatous response.
authors.affiliation: Laboratório da Disciplina de Patologia de Moléstias Transmissíveis, Departamento de Patologia, Faculdade de Medicina da Universidade de São Paulo, Brazil; Laboratório de Dermatopatologia, Departamento de Dermatologia, Faculdade de Medicina da Universidade de São Paulo, Brazil.
authors.forename: Aline Alves de Lima
authors.initials: AA
authors.lastname: Silva
authors.forename: Mirian N
authors.initials: MN
authors.lastname: Sotto
authors.forename: Maria Irma Seixas
authors.initials: MI
authors.lastname: Duarte
authors.forename: Carla
authors.initials: C
authors.lastname: Pagliari
mesh_terms: D000328:Adult; D000946:Antigens, Fungal / Q000276:immunology; D005260:Female; D051858:Forkhead Transcription Factors / Q000276:immunology; D006099:Granuloma / Q000276:immunology / Q000382:microbiology; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology; D016753:Interleukin-10 / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D008297:Male; D009092:Mucous Membrane / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D010228:Paracoccidioides / Q000276:immunology*; D010229:Paracoccidioidomycosis / Q000276:immunology* / Q000382:microbiology; D012867:Skin / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000276:immunology
chemical_list: D000946:Antigens, Fungal; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; C508609:IL10 protein, human; C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit; D016212:Transforming Growth Factor beta; D016753:Interleukin-10
keywords: Cytokines; Paracoccidioidomycosis; Regulatory T cells; Skin