title: Live pigs produced from genome edited zygotes.
abstract: Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology enables site directed engineering of the genome. Here we demonstrate for the first time that both TALEN and ZFN injected directly into pig zygotes can produce live genome edited pigs. Monoallelic as well as heterozygous and homozygous biallelic events were identified, significantly broadening the use of genome editor technology in livestock by enabling gene knockout in zygotes from any chosen mating.
authors.affiliation: The Roslin Institute and R(D)SVS, Easter Bush Campus, University of Edinburgh, Edinburgh, EH25 9RG, UK.
authors.forename: Simon G
authors.initials: SG
authors.lastname: Lillico
authors.forename: Chris
authors.initials: C
authors.lastname: Proudfoot
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Carlson
authors.forename: Dana
authors.initials: D
authors.lastname: Stverakova
authors.forename: Claire
authors.initials: C
authors.lastname: Neil
authors.forename: Carol
authors.initials: C
authors.lastname: Blain
authors.forename: Tim J
authors.initials: TJ
authors.lastname: King
authors.forename: William A
authors.initials: WA
authors.lastname: Ritchie
authors.forename: Wenfang
authors.initials: W
authors.lastname: Tan
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Mileham
authors.forename: David G
authors.initials: DG
authors.lastname: McLaren
authors.forename: Scott C
authors.initials: SC
authors.lastname: Fahrenkrug
authors.forename: C Bruce A
authors.initials: CB
authors.lastname: Whitelaw
mesh_terms: D000483:Alleles; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D055786:Gene Knockout Techniques; D005818:Genetic Engineering; D016678:Genome; D006720:Homozygote; D008969:Molecular Sequence Data; D053652:Nuclear Transfer Techniques; D017393:RNA Editing / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D016335:Zinc Fingers / Q000235:genetics*; D015053:Zygote / Q000166:cytology* / Q000378:metabolism
chemical_list: D004720:Endonucleases