title: Genomic DNA isolation of Acrocomia aculeata (Arecaceae) from leaf and stipe tissue samples for PCR analysis.
abstract: Macaw palm, Acrocomia aculeata is an oleaginous species of the Arecaceae family; it has been identified as one of the most promising plants for sustainable production of renewable energy, especially biodiesel. We developed an efficient protocol of genomic DNA extraction for A. aculeata using leaf and stipe tissues, based on the cationic hexadecyltrimethylammonium bromide method, and we evaluated the quantity, purity, and integrity of the resultant DNA. We also determined whether these procedures interfere with PCR amplification using SSR molecular markers. The lowest concentration of DNA was obtained from stipe tissues (135 ng/μL), while fresh leaf tissues provided the highest concentration of DNA (650 ng/μL). Good quality DNA was obtained from fresh leaf, lyophilized leaf, and stipe tissues (relative purity, 1.79-1.89 nm). Differences in quantity and quality of DNA extracted from different tissues did not interfere with general patterns of PCR amplification based on SSR markers.
authors.affiliation: Departamento de Fitotecnia, Universidade Federal de Viçosa, Viçosa, MG, Brasil edercml@gmail.com.
authors.forename: E C M
authors.initials: EC
authors.lastname: Lanes
authors.forename: C
authors.initials: C
authors.lastname: Nick
authors.forename: K N
authors.initials: KN
authors.lastname: Kuki
authors.forename: R D
authors.initials: RD
authors.lastname: Freitas
authors.forename: S Y
authors.initials: SY
authors.lastname: Motoike
mesh_terms: D028023:Arecaceae / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification*; D005819:Genetic Markers; D018895:Microsatellite Repeats; D021141:Nucleic Acid Amplification Techniques; D018515:Plant Leaves / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers