title: Effects of storage temperature and semen extender on stored canine semen.
abstract: The objective of the present study was to determine an optimum temperature and extender for short-term transport of canine ejaculated semen. There was no significant difference in the qualities of semen diluted with two kinds of extender, egg yolk Tris-citrate fructose (EYT-FC) or glucose (EYT-GC) extender, between the 2, 8 or 12 and the 4°C control groups during storage for up to 48 hr, while the 16-24°C groups showed decreased sperm motility during storage for 48 hr. However, the 2°C group showed slightly lower sperm motility and slightly higher sperm abnormality than the 4°C group. Therefore, we concluded that semen qualities can be maintained for up to 48 hr when canine semen samples are extended with EYT-FC or EYT-GC and stored at a temperature in the range of 4-12°C.
authors.affiliation: Department of Reproduction, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Hori
authors.forename: Ryuta
authors.initials: R
authors.lastname: Yoshikuni
authors.forename: Masanori
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Eiichi
authors.initials: E
authors.lastname: Kawakami
mesh_terms: D000818:Animals; D019343:Citric Acid; D004285:Dogs; D004530:Egg Yolk; D005632:Fructose; D005947:Glucose; D008297:Male; D012661:Semen / Q000166:cytology*; D055101:Semen Analysis / Q000379:methods; D012662:Semen Preservation / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D019343:Citric Acid; D005632:Fructose; D005947:Glucose