title: [Effects of composite resin and glass-ionomer cements on proliferation and functional activity of human macrophages].
abstract: OBJECTIVE
  To study the effects of composite resin and glass-ionomer cements on cell proliferation and function of human macrophages in vitro.
  
  
  METHODS
  Macrophages were differentiated from THP-1 cells after treatment with phorbol ester and used as the model of inflammatory cells, which were treated by specimens from glass-ionomer cements(GC), composite resin Filtek Z350 (3M) and Filtek P60(3M) on culture medium for 24 hours. The cell proliferation of the tooth-colored restorative materials on human macrophages in vitro was evaluated by MTT color imetric assay, and determined for IL-1 content in these material specimens by ELISA. All statistical analyses were performed using the SPSS 17.0 software package.
  
  
  RESULTS
  Compared with control group, composite resin Filtek Z350(3M) and Filtek P60(3M) significantly enhanced the proliferation of human macrophages (P<0.05), while Glass-ionomer had little effect on the proliferation of human macrophages (P>0.05). Glass-ionomer could promote macrophages to secrete IL-1β and the difference was statistically significant(P<0.05). The composite resin could not cause release of IL-1β from macrophages (P>0.05).
  
  
  CONCLUSIONS
  Composite resin enhances proliferation and function of human macrophages. The effect may be associated with hypersensitivity of dentin. Glass-ionomer cement has little effect on proliferation of macrophages, but may lead to progress of inflammation.
authors.affiliation: Department of Emergency, Affiliated Stomatological Hospital of Kunming Medical University. Kunming 650031;China.E-mail:318731210@qq.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Gong
authors.forename: Rong-qiang
authors.initials: RQ
authors.lastname: Yang
authors.forename: Hong-li
authors.initials: HL
authors.lastname: Yang
mesh_terms: D000180:Acrylic Resins; D049109:Cell Proliferation; D003188:Composite Resins; D003764:Dental Materials; D003804:Dentin; D005899:Glass Ionomer Cements; D006801:Humans; D008264:Macrophages; D012822:Silicon Dioxide
chemical_list: D000180:Acrylic Resins; D003188:Composite Resins; D003764:Dental Materials; D005899:Glass Ionomer Cements; C533204:filtek Z350; C015897:glass ionomer; D012822:Silicon Dioxide