title: Analysis of neuronal cells of dissociated primary culture on high-density CMOS electrode array.
abstract: Spontaneous development of neuronal cells was recorded around 4-34 days in vitro (DIV) with high-density CMOS array, which enables detailed study of the spatio-temporal activity of neuronal culture. We used the CMOS array to characterize the evolution of the inter-spike interval (ISI) distribution from putative single neurons, and estimate the network structure based on transfer entropy analysis, where each node corresponds to a single neuron. We observed that the ISI distributions gradually obeyed the power law with maturation of the network. The amount of information transferred between neurons increased at the early stage of development, but decreased as the network matured. These results suggest that both ISI and transfer entropy were very useful for characterizing the dynamic development of cultured neural cells over a few weeks.
authors.forename: Eiko
authors.initials: E
authors.lastname: Matsuda
authors.forename: Takeshi
authors.initials: T
authors.lastname: Mita
authors.forename: Julien
authors.initials: J
authors.lastname: Hubert
authors.forename: Douglas
authors.initials: D
authors.lastname: Bakkum
authors.forename: Urs
authors.initials: U
authors.lastname: Frey
authors.forename: Andreas
authors.initials: A
authors.lastname: Hierlemann
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Takahashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Ikegami
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D002478:Cells, Cultured; D004566:Electrodes; D019277:Entropy; D008670:Metals / Q000737:chemistry*; D009415:Nerve Net / Q000502:physiology; D016571:Neural Networks, Computer; D009474:Neurons / Q000502:physiology*; D010087:Oxides / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012666:Semiconductors
chemical_list: D008670:Metals; D010087:Oxides