title: Dissecting functions of the conserved oligomeric Golgi tethering complex using a cell-free assay.
abstract: Vesicle transport sorts proteins between compartments and is thereby responsible for generating the non-uniform protein distribution along the eukaryotic secretory and endocytic pathways. The mechanistic details of specific vesicle targeting are not yet well characterized at the molecular level. We have developed a cell-free assay that reconstitutes vesicle targeting utilizing the recycling of resident enzymes within the Golgi apparatus. The assay has physiological properties, and could be used to show that the two lobes of the conserved oligomeric Golgi tethering complex play antagonistic roles in trans-Golgi vesicle targeting. Moreover, we can show that the assay is sensitive to several different congenital defects that disrupt Golgi function and therefore cause glycosylation disorders. Consequently, this assay will allow mechanistic insight into the targeting step of vesicle transport at the Golgi, and could also be useful for characterizing some novel cases of congenital glycosylation disorders.
authors.affiliation: Department of Biology, University of York, York, UK.
authors.forename: Nathanael P
authors.initials: NP
authors.lastname: Cottam
authors.forename: Katherine M
authors.initials: KM
authors.lastname: Wilson
authors.forename: Bobby G
authors.initials: BG
authors.lastname: Ng
authors.forename: Christian
authors.initials: C
authors.lastname: Körner
authors.forename: Hudson H
authors.initials: HH
authors.lastname: Freeze
authors.forename: Daniel
authors.initials: D
authors.lastname: Ungar
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism; D002474:Cell-Free System / Q000378:metabolism*; D005700:Galactosyltransferases / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D055503:Protein Multimerization; D021381:Protein Transport; D022142:Secretory Vesicles / Q000378:metabolism
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D005700:Galactosyltransferases; C119701:beta-1,4-galactosyltransferase I
keywords: Golgi apparatus; cell-free reconstitution; congenital disorders of glycosylation; conserved oligomeric Golgi complex; glycosylation enzyme sorting; vesicle tethering