title: Expression, purification and preliminary X-ray crystallographic analysis of Arf1-GDP in complex with dimeric p23 peptide.
abstract: Arf1 is a member of the Ras superfamily and is involved in COPI vesicle formation. Arf1-GDP can interact with dimeric p23. Here, human Arf1 (residues 18-181) was cloned, expressed and purified in Escherichia coli. For crystallization, Arf1-GDP was mixed with dimeric p23 peptide in a 1:5 molar ratio. Crystals were obtained which diffracted to 2.7 Å resolution. The crystals belonged to space group P6₁22, with unit-cell parameters a=b=80.6, c=336.0 Å, α=β=90, γ=120°. The asymmetric unit of the crystals contained two molecules, with a Matthews coefficient of 3.2 Å3 Da(-1) and a solvent content of 61.9%.
authors.affiliation: Laboratory of Noncoding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zheng
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Kai
authors.initials: K
authors.lastname: Deng
authors.forename: Weimin
authors.initials: W
authors.lastname: Gong
authors.forename: Zhe
authors.initials: Z
authors.lastname: Sun
mesh_terms: D020823:ADP-Ribosylation Factor 1 / Q000737:chemistry* / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D018360:Crystallography, X-Ray; D006153:Guanosine Diphosphate / Q000737:chemistry*; D006801:Humans; D010455:Peptides / Q000737:chemistry*; D055503:Protein Multimerization
chemical_list: D010455:Peptides; D006153:Guanosine Diphosphate; D020823:ADP-Ribosylation Factor 1
keywords: Arf1; COPI; dimer; p23