title: [Expression and clinical significance of E-cadherin and β-catenin in the vulvar squamous cell carcinoma tissues].
abstract: OBJECTIVE
  To investigate the expression of E-cadherin and β-catenin in the Vulvar squamous cell carcinoma (VSCC) tissues.
  
  
  METHODS
  A total of 63 documented paraffin blocks of VSCC (n=41), vulvar intraepithelial neoplasia (n=22), vulvar negative cutting edge tissues (n=10) diagnosed in department of pathology of Shengjing Hospital of China Medical University from January 2005 to April 2012 were enrolled. EliVision immunohistochemical staining was used to detect the expression of E-cadherin and β-catenin in the three groups. Then to do a statistical analysis among the expression of them with patients' menopause status, pathological grade, clinical stage and lymph node metastasis. Spearman correlation analysis was used to analyse the expression of E-cadherin and β-catenin in the vulvar lesion tissues.
  
  
  RESULTS
  The abnormal immunoreactivity for E-cadherin [46%(19/41), 64% (14/22)] and β-catenin [61% (25/41), 68% (15/22)] in VSCC and VINII-III were found, which were significantly different from that in normal epithelium samples (P<0.05). The abnormal expression of E-cadherin and β-catenin have no statistically significant difference between VSCC group and VINII-III group (P>0.05). The abnormal expression of E-cadherin and β-catenin were collected with tumor pathological grade and lymph node metastasis status (all P<0.05). The abnormal expression of E-cadherin and β-catenin have no statistically significant difference between menopause and the surgical stage of patients (all P>0.05). The abnormal expression of E-cadherin and β-catenin have a significant positive correlation in the same sample in the VSCC tissue (r=0.543, P=0.000). The abnormal expression of E-cadherin and β-catenin have no correlation in the VINII-III tissue (r=0.295, P=0.182).
  
  
  CONCLUSIONS
  The abnormal expression of E-cadherin and β-catenin may occurs frequently in the VSCC. The abnormal expression of E-cadherin and β-catenin have correlation with vulvar cancer pathological grade and lymph node metastasis, which may be important mechanisms promoting the invasion and metastasis of VSCC.
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Jia
authors.forename: Ling
authors.initials: L
authors.lastname: Ouyang
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Zi-xuan
authors.initials: ZX
authors.lastname: Song
authors.forename: Xin
authors.initials: X
authors.lastname: Tong
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Shi-tai
authors.initials: ST
authors.lastname: Zhang
authors.forename: Zheng
authors.initials: Z
authors.lastname: He
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis / Q000473:pathology; D008875:Middle Aged; D009367:Neoplasm Staging; D018307:Neoplasms, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D014846:Vulvar Neoplasms / Q000378:metabolism* / Q000473:pathology; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; C495270:CTNNB1 protein, human; D015820:Cadherins; D051176:beta Catenin