title: Micromonospora polyrhachis sp. nov., an actinomycete isolated from edible Chinese black ant (Polyrhachis vicina Roger).
abstract: A novel actinomycete, designated strain NEAU-ycm2(T), was isolated from edible Chinese black ants (Polyrhachis vicina Roger) and characterized using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of the genus Micromonospora. The 16S rRNA gene sequence of strain NEAU-ycm2(T) showed highest similarity to those of Micromonospora sonneratiae 274745(T) (99.12%), Micromonospora pattaloongensis TJ2-2(T) (98.85%), Micromonospora pisi GUI 15(T) (98.76%), Polymorphospora rubra TT 97-42(T) (98.42%) and Micromonospora eburnea LK2-10(T) (98.21%). Phylogenetic analysis based on the 16S rRNA gene and gyrB gene demonstrated that strain NEAU-ycm2(T) is a member of the genus Micromonospora and supported the close phylogenetic relationship to M. sonneratiae 274745(T), M. pattaloongensis JCM 12833(T) and M. pisi GUI 15(T). Furthermore, a combination of DNA-DNA hybridization and some physiological and biochemical properties indicated that the novel strain could be readily distinguished from its closest phylogenetic relatives. Therefore, it is proposed that NEAU-ycm2(T) represents a novel species of the genus of Micromonospora, for which the name Micromonospora polyrhachis sp. nov. is proposed. The type strain is NEAU-ycm2(T) ( = CGMCC 4.7100(T) = DSM 45886(T)).
authors.affiliation: School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Xiang
authors.affiliation: School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yu
authors.affiliation: School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Chongxi
authors.initials: C
authors.lastname: Liu
authors.affiliation: School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Zhao
authors.affiliation: School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Lingyu
authors.initials: L
authors.lastname: Yang
authors.affiliation: School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Binjiao
authors.initials: B
authors.lastname: Xie
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, PR China.
authors.forename: Kui
authors.initials: K
authors.lastname: Hong
authors.affiliation: School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Xiangjing
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D001000:Ants / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D008847:Micromonospora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2