title: High expression of H3K27me3 is an independent predictor of worse outcome in patients with urothelial carcinoma of bladder treated with radical cystectomy.
abstract: It has been suggested that trimethylation of lysine 27 on histone H3 (H3K27me3) is a crucial epigenetic process in tumorigenesis. However, the expression pattern of H3K27me3 and its clinicopathological/prognostic significance in urothelial carcinoma of bladder (UCB) are unclear. In this study, upregulated expression of H3K27me3 protein was observed in the majority of UCBs by Western blotting. High expression of H3K27me3 was examined by IHC in 59/126 (46.8%) of UCB tissues and in 18/72 (25.0%) of normal urothelial bladder epithelial tissues (P = 0.002). High expression of H3K27me3 was associated with multifocal tumors and lymph node metastases (P < 0.05). Patients with high expression of H3K27me3 had shorter cancer-specific survival (CSS) time than patients with low expression of H3K27me3 (P < 0.001). In different subsets of UCB patients, high expression of H3K27me3 was also a prognostic indicator in patients with grade 2 and grade 3, pT1, pT2, pT3, and pN- disease (P < 0.05). Importantly, expression of H3K27me3 was an independent predictor for CSS (P < 0.001) of UCB patients treated with radical cystectomy (RC). Our data suggests that high expression of H3K27me3 is an independent molecular marker for predicting poor prognosis of UCB patients treated with RC.
authors.affiliation: State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, China ; Department of Urology, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, China.
authors.forename: Jianye
authors.initials: J
authors.lastname: Liu
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Li
authors.forename: Yiji
authors.initials: Y
authors.lastname: Liao
authors.forename: Shijuan
authors.initials: S
authors.lastname: Mai
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhouwei
authors.initials: Z
authors.lastname: Liu
authors.forename: Lijuan
authors.initials: L
authors.lastname: Jiang
authors.forename: Yixin
authors.initials: Y
authors.lastname: Zeng
authors.forename: Fangjian
authors.initials: F
authors.lastname: Zhou
authors.forename: Dan
authors.initials: D
authors.lastname: Xie
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis; D002277:Carcinoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D015653:Cystectomy / Q000009:adverse effects; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D056466:Histone Demethylases / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics* / Q000473:pathology; D008297:Male; D008745:Methylation; D008875:Middle Aged; D011379:Prognosis; D016896:Treatment Outcome; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000601:surgery
chemical_list: D014408:Biomarkers, Tumor; D056466:Histone Demethylases