title: Endostatin/collagen XVIII is increased in cerebrospinal fluid after severe traumatic brain injury.
abstract: Recent studies have suggested that endogenous angiogenesis inhibitor endostatin/collagen XVIII might play an important role in the secondary brain injury following traumatic brain injury (TBI). In this study, we measured endostatin/collagen XVIII concentrations serially for 1 week after hospitalization by using the enzyme-linked immunosorbent assay method in the cerebrospinal fluid (CSF) of 30 patients with TBI and a Glasgow Coma Scale (GCS) score of 8 or less on admission. There was a significant trend toward increased CSF levels of endostatin after TBI versus control from 72 h after injury. In patients with GCS score of 3-5, CSF endostatin concentration was substantially higher at 72 h after injury than that in patients with GCS score of 6-8 (P < 0.05) and peaked rapidly at day 5 after injury, but decreased thereafter. The CSF endostatin concentration in 12 patients with an unfavorable outcome was significantly higher than that in 18 patients with a favorable outcome at day 5 (P = 0.043) and day 7 (P = 0.005) after trauma. Receiver operating characteristic curve analysis suggested a reliable operating point for the 7-day CSF endostatin concentration predicting poor prognosis to be 67.29 pg/mL. Our preliminary findings provide new evidence that endostatin/collagen XVIII concentration in the CSF increases substantially in patients with sTBI. Its dynamic change may have some clinical significance on the judgment of brain injury severity and the assessment of prognosis. This trial is registered with the ClinicalTrials.gov Identifier: NCT01846546.
authors.affiliation: Department of Neurosurgery, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, 600 Yishan Road, Shanghai 200233, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Xue
authors.forename: He-Li
authors.initials: HL
authors.lastname: Cao
authors.forename: Shi-Wen
authors.initials: SW
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.forename: Wen-Wei
authors.initials: WW
authors.lastname: Gao
authors.forename: Shi-Ming
authors.initials: SM
authors.lastname: Ju
authors.forename: Heng-Li
authors.initials: HL
authors.lastname: Tian
mesh_terms: D000368:Aged; D001930:Brain Injuries / Q000134:cerebrospinal fluid* / Q000503:physiopathology*; D043170:Collagen Type XVIII / Q000134:cerebrospinal fluid* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015600:Glasgow Coma Scale; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012372:ROC Curve
chemical_list: D043170:Collagen Type XVIII