title: Sites under positive selection modulate the RNA silencing suppressor activity of rice yellow mottle virus movement protein P1.
abstract: RNA silencing is a eukaryotic mechanism for RNA-based gene regulation that plays an essential role in diverse biological processes, such as defence against viral infections. The P1 of rice yellow mottle virus (RYMV) is a movement protein and displays RNA silencing suppression activity with variable efficiency, depending on the origin of the isolates. In this study, the positive selection pressure acting on the P1 protein gene was assessed. A site-by-site analysis of the dN/dS ratio was performed and 18 positively selected sites were identified. Four of these were mutated, and the ability to suppress RNA silencing was evaluated for the resulting mutants in a transient expression assay. All mutations affected quantitatively RNA silencing suppression, one caused a significant decrease in the activity and three significantly increased it. This work demonstrates, for what is to the best of our knowledge the first time, that the RYMV gene encoding the P1 RNA silencing suppressor is under adaptive evolution.
authors.affiliation: Institut de l'Environnement et de Recherches Agricoles (INERA), Laboratoire de Virologie et de Biotechnologies Végétales, 01 BP 476 Ouagadougou 01, Burkina Faso.
authors.forename: Drissa
authors.initials: D
authors.lastname: Sérémé
authors.affiliation: Institut de Recherche pour le Développement (IRD), UMR 186/RPB, 911 Av. Agropolis BP 64501 F-34394 Montpellier Cedex 5, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Lacombe
authors.affiliation: Institut de l'Environnement et de Recherches Agricoles (INERA), Laboratoire de Virologie et de Biotechnologies Végétales, 01 BP 476 Ouagadougou 01, Burkina Faso.
authors.forename: Moumouni
authors.initials: M
authors.lastname: Konaté
authors.affiliation: Institut de Recherche pour le Développement (IRD), UMR 186/RPB, 911 Av. Agropolis BP 64501 F-34394 Montpellier Cedex 5, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Bangratz
authors.affiliation: Institut de Recherche pour le Développement (IRD), UMR 186/RPB, 911 Av. Agropolis BP 64501 F-34394 Montpellier Cedex 5, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Pinel-Galzi
authors.affiliation: Institut de Recherche pour le Développement (IRD), UMR 186/RPB, 911 Av. Agropolis BP 64501 F-34394 Montpellier Cedex 5, France.
authors.forename: Denis
authors.initials: D
authors.lastname: Fargette
authors.affiliation: Université de Ouagadougou, UFR SVT, 03 BP 7021 Ouagadougou 03, Burkina Faso.
authors.forename: Alfred S
authors.initials: AS
authors.lastname: Traoré
authors.affiliation: Institut de l'Environnement et de Recherches Agricoles (INERA), Laboratoire de Virologie et de Biotechnologies Végétales, 01 BP 476 Ouagadougou 01, Burkina Faso.
authors.forename: Gnissa
authors.initials: G
authors.lastname: Konaté
authors.affiliation: Institut de Recherche pour le Développement (IRD), UMR 186/RPB, 911 Av. Agropolis BP 64501 F-34394 Montpellier Cedex 5, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Brugidou
mesh_terms: D000595:Amino Acid Sequence; D019143:Evolution, Molecular; D054884:Host-Pathogen Interactions; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000821:virology; D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D034622:RNA Interference; D012328:RNA Viruses / Q000235:genetics / Q000378:metabolism*; D012641:Selection, Genetic; D016415:Sequence Alignment; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C112015:P1 protein, Rice yellow mottle virus; D014764:Viral Proteins