title: Biodegradation and cometabolic modeling of selected beta blockers during ammonia oxidation.
abstract: Accurate prediction of pharmaceutical concentrations in wastewater effluents requires that the specific biochemical processes responsible for pharmaceutical biodegradation be elucidated and integrated within any modeling framework. The fate of three selected beta blockers-atenolol, metoprolol, and sotalol-was examined during nitrification using batch experiments to develop and evaluate a new cometabolic process-based (CPB) model. CPB model parameters describe biotransformation during and after ammonia oxidation for specific biomass populations and are designed to be integrated within the Activated Sludge Models framework. Metoprolol and sotalol were not biodegraded by the nitrification enrichment culture employed herein. Biodegradation of atenolol was observed and linked to the activity of ammonia-oxidizing bacteria (AOB) and heterotrophs but not nitrite-oxidizing bacteria. Results suggest that the role of AOB in atenolol degradation may be disproportionately more significant than is otherwise suggested by their lower relative abundance in typical biological treatment processes. Atenolol was observed to competitively inhibit AOB growth in our experiments, though model simulations suggest inhibition is most relevant at atenolol concentrations greater than approximately 200 ng·L(-1). CPB model parameters were found to be relatively insensitive to biokinetic parameter selection suggesting the model approach may hold utility for describing pharmaceutical biodegradation during biological wastewater treatment.
authors.affiliation: Tufts University , Department of Civil and Environmental Engineering, 200 College Avenue Room 113 Anderson Hall, Medford, Massachusetts 02155, United States.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Sathyamoorthy
authors.forename: Kartik
authors.initials: K
authors.lastname: Chandran
authors.forename: C Andrew
authors.initials: CA
authors.lastname: Ramsburg
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000378:metabolism*; D000641:Ammonia / Q000378:metabolism*; D001262:Atenolol / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D018533:Biomass; D008954:Models, Biological; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D010084:Oxidation-Reduction; D012996:Solutions
chemical_list: D000319:Adrenergic beta-Antagonists; D009566:Nitrates; D009573:Nitrites; D012996:Solutions; D001262:Atenolol; D000641:Ammonia