title: Autocrine and paracrine modulation of microRNA-155 expression by globular adiponectin in RAW 264.7 macrophages: involvement of MAPK/NF-κB pathway.
abstract: Adiponectin, a hormone produced from adipose tissue, regulates various biological responses, including inflammation and many metabolic processes. MicroRNAs control expression of diverse target genes and various physiological responses. Many of these responses are commonly regulated by adiponectin. However, effects of adiponectin on microRNAs regulation are largely unknown. Herein we demonstrated that globular adiponectin induces increase in miR-155 expression, which plays an important role in inflammatory response, in RAW 264.7 macrophages. We further showed that this effect was modulated by and MAPK/NF-κB dependent mechanisms. These results suggest that miR-155 would be a novel promising target mediating adiponectin-induced various biological responses.
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsanbuk-do 712-749, Republic of Korea.
authors.forename: Amit
authors.initials: A
authors.lastname: Subedi
authors.forename: Pil-Hoon
authors.initials: PH
authors.lastname: Park
mesh_terms: D052242:Adiponectin / Q000235:genetics / Q000494:pharmacology*; D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D019898:Autocrine Communication / Q000187:drug effects; D002460:Cell Line; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology; D019899:Paracrine Communication / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013450:Sulfones / Q000494:pharmacology; D013997:Time Factors
chemical_list: C434003:3-(4-methylphenylsulfonyl)-2-propenenitrile; D052242:Adiponectin; D000873:Anthracenes; D017077:Culture Media, Conditioned; D035683:MicroRNAs; C532623:Mirn155 microRNA, mouse; D016328:NF-kappa B; D009570:Nitriles; D011994:Recombinant Proteins; D013450:Sulfones; C432165:pyrazolanthrone; D048031:JNK Mitogen-Activated Protein Kinases
keywords: Adiponectin; Autocrine; Macrophages; MicroRNA; Paracrine