title: The in vivo effect of P-15 coating on early osseointegration.
abstract: The aim of this study was to evaluate mechanically and morphologically the effect of a specific peptide sequence P-15, when incorporated into implant surfaces. Three types of implants were used for the study: Group A: commercially pure titanium implant (blasted and acid etched) + electrochemical thin calcium phosphate deposition, Group B: commercially pure titanium implant (blasted and acid etched) + electrochemical thin calcium phosphate deposition + P-15 incorporation, and as control, Group C: commercially pure titanium implant (blasted and acid etched). After a topographical characterization, transcortical osteotomies were made, and all implant groups (102 implants per group) were randomly placed bilaterally in the tibiae of adult beagle dogs (n = 24). At, 1, 2, and 4 weeks post-surgery, the animals were sacrificed and the samples were retrieved for removal torque tests, for nano indentation, and for histomorphometrical analysis. The results (mean ± 95% CI) showed that Group B (34.4 ± 8.7%) presented statistically higher bone-to-implant contact than the other groups (A = 23.9 ± 7.8%; C = 21.7 ± 8.3%) at 1 week, indicating an enhanced osteogenesis due to the peptide incorporation. The results suggested that the incorporation of P-15 to implant surfaces increased its bioactivity and the effects were notable especially in the early stages of the healing process.
authors.affiliation: Department of Biomaterials and Biomimetics, New York University College of Dentistry, New York; Department of Periodontology and Implant Dentistry, New York University College of Dentistry, New York.
authors.forename: Paulo G
authors.initials: PG
authors.lastname: Coelho
authors.forename: Hellen S
authors.initials: HS
authors.lastname: Teixeira
authors.forename: Charles
authors.initials: C
authors.lastname: Marin
authors.forename: Lukasz
authors.initials: L
authors.lastname: Witek
authors.forename: Nick
authors.initials: N
authors.lastname: Tovar
authors.forename: Malvin N
authors.initials: MN
authors.lastname: Janal
authors.forename: Ryo
authors.initials: R
authors.lastname: Jimbo
mesh_terms: D000465:Algorithms; D000818:Animals; D001696:Biomechanical Phenomena; D003094:Collagen / Q000494:pharmacology*; D016001:Confidence Intervals; D004285:Dogs; D004563:Electrochemistry; D005557:Formaldehyde; D007368:Interferometry; D007595:Joint Prosthesis; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration / Q000187:drug effects*; D010446:Peptide Fragments / Q000494:pharmacology*; D013977:Tibia / Q000254:growth & development / Q000502:physiology; D019415:Torque
chemical_list: D010446:Peptide Fragments; C407062:cell-binding peptide P-15; D005557:Formaldehyde; D003094:Collagen
keywords: bioactivity; biomechanics; histomorphometry; in vivo; peptide