title: Toll-like receptor 2 subfamily genotypes are not associated with severity of bronchiolitis or postbronchiolitis wheezing in infants.
abstract: OBJECTIVE
  Expression of toll-like receptor (TLR) 2 subfamily genes, including genes encoding TLR1, TLR2, TLR6 and TLR10, have been connected to allergy and asthma. This controlled study investigated the association of TLR1, TLR2 and TLR6 gene polymorphisms with clinical characteristics and subsequent wheezing in young infants with bronchiolitis.
  
  
  METHODS
  In all, 129 full-term infants hospitalised for bronchiolitis at the age of <6 months were clinically followed up until a mean age of 18 months. Genotyping of the TLR1 T1805G, TLR2 G2258A and TLR6 C745T polymorphisms was carried out by pyrosequencing and in 318 healthy, Finnish controls.
  
  
  RESULTS
  There were no significant associations between TLR1, TLR2 or TLR6 genotypes and severity of bronchiolitis or risk of postbronchiolitis wheezing. TLR6 polymorphism was associated with allergy in univariate analyses. Minor allele frequency (MAF) in the TLR1 gene (17%) in the hospitalised children was similar to our Finnish controls, but different to European controls from other studies. MAF in the TLR6 gene was 50% versus 41% in both the Finnish and European controls. MAF in the TLR2 gene was low (3%) in study subjects and in both controls.
  
  
  CONCLUSIONS
  TLR2 subfamily gene polymorphisms were not associated with severity of bronchiolitis or risk of postbronchiolitis wheezing.
authors.affiliation: Seinäjoki Central Hospital, Seinäjoki, Finland. kirsi.nuolivirta@fimnet.fi.
authors.forename: Kirsi
authors.initials: K
authors.lastname: Nuolivirta
authors.forename: Juho
authors.initials: J
authors.lastname: Vuononvirta
authors.forename: Ville
authors.initials: V
authors.lastname: Peltola
authors.forename: Petri
authors.initials: P
authors.lastname: Koponen
authors.forename: Merja
authors.initials: M
authors.lastname: Helminen
authors.forename: Qiushui
authors.initials: Q
authors.lastname: He
authors.forename: Matti
authors.initials: M
authors.lastname: Korppi
mesh_terms: D001988:Bronchiolitis / Q000235:genetics*; D016022:Case-Control Studies; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007223:Infant; D020641:Polymorphism, Single Nucleotide; D012135:Respiratory Sounds / Q000235:genetics*; D051194:Toll-Like Receptor 1 / Q000235:genetics*; D051195:Toll-Like Receptor 2 / Q000235:genetics*; D051216:Toll-Like Receptor 6 / Q000235:genetics*
chemical_list: C495342:TLR2 protein, human; C495368:TLR6 protein, human; D051194:Toll-Like Receptor 1; D051195:Toll-Like Receptor 2; D051216:Toll-Like Receptor 6
keywords: Bronchiolitis; Infant; Toll-like receptor 1; Toll-like receptor 2; Toll-like receptor 2 subfamily; Toll-like receptor 3; Wheezing