title: In vitro and in vivo effects of (-)-epigallocatechin 3-O-gallate on Leishmania amazonensis.
abstract: (-)-Epigallocatechin 3-O-gallate (1), the most abundant flavanol in green tea, has been reported to have antiproliferative effects on Trypanosoma cruzi. The present study reports the effects in vitro and in vivo of 1 on Leishmania amazonensis. L. amazonensis-infected macrophages treated with 1 exhibited a significant reduction of the infection index in a dose-dependent manner, with an IC50 value of 1.6 μM. Oral administration of 1 on L. amazonensis-infected BALB/c mice (30 mg/kg/day) resulted in a decrease in the lesion size and parasite burden, without altering serological markers of toxicity. These data demonstrate the in vitro and in vivo leishmanicidal effects of compound 1.
authors.affiliation: Laboratório de Bioquímica de Tripanosomatideos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz , Pavilhão Leônidas Deane, Manguinhos, 21045-900, Rio de Janeiro, Brazil.
authors.forename: Job D F
authors.initials: JD
authors.lastname: Inacio
authors.forename: Marilene M
authors.initials: MM
authors.lastname: Canto-Cavalheiro
authors.forename: Elmo E
authors.initials: EE
authors.lastname: Almeida-Amaral
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001938:Brazil; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D020128:Inhibitory Concentration 50; D007894:Leishmania mexicana / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D013237:Stereoisomerism; D013662:Tea / Q000737:chemistry; D014349:Trypanosoma cruzi / Q000187:drug effects
chemical_list: D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate