title: The heterogeneous immune microenvironment in breast cancer is affected by hypoxia-related genes.
abstract: The immune system constitutes an important first-line defence against malignant transformation. However, cancer mediated immunosuppression inactivates the mechanisms of host immune surveillance. Cancer cells shut down anti-cancer immunity through direct cell-cell interactions with leukocytes and through soluble factors, establishing an immunosuppressive environment for unimpeded cancer growth. The composition of the immunosuppressive microenvironment in breast tumours is not well documented. To address this question, selected immunosuppressive factors were analyzed in tumour specimens from 33 breast cancer patients after surgery. The mRNA expression of selected genes was quantified in fresh tumour samples. Tumour infiltrating leukocytes were characterized by flow cytometry to identify regulatory T cells, myeloid derived suppressor cells, and type 2 macrophages. Statistical analysis revealed several interesting correlations between the studied parameters and clinical features. Overall, a surprisingly high degree of heterogeneity in the composition of the immunosuppressive environment was found across all breast cancer samples which adds to the complexity of this disease. The influence of the hypoxia inducible factors (HIFs) on the immune microenvironment was also addressed. The level of HIFs correlated with hormone receptor status and the expression of several immunosuppressive molecules. Targeting HIFs might not only sensitize breast tumours for radiation and chemotherapies but also interfere with cancer immunosuppression.
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, 112 Sienkiewicza Street, 90-363 Lodz, Poland. Electronic address: mduchler@cbmm.lodz.pl.
authors.forename: Markus
authors.initials: M
authors.lastname: Duechler
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, 112 Sienkiewicza Street, 90-363 Lodz, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Peczek
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, 112 Sienkiewicza Street, 90-363 Lodz, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Zuk
authors.affiliation: Department of Molecular Biology of Cancer, Medical University of Lodz, 6/8 Mazowiecka Street, 92-215 Lodz, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Zalesna
authors.affiliation: Clinics for Oncological Surgery, Medical University of Lodz, 62 Pabianicka Street, 93-513 Lodz, Poland.
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Jeziorski
authors.affiliation: Department of Molecular Biology of Cancer, Medical University of Lodz, 6/8 Mazowiecka Street, 92-215 Lodz, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Czyz
mesh_terms: D000368:Aged; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D001943:Breast Neoplasms / Q000235:genetics / Q000276:immunology*; D002277:Carcinoma / Q000235:genetics / Q000276:immunology*; D002469:Cell Separation; D002478:Cells, Cultured; D060833:Cellular Microenvironment; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D007157:Immunologic Surveillance; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008875:Middle Aged; D058990:Molecular Targeted Therapy; D022423:Myeloid Cells / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D019139:Tumor Escape; D000099162:Endothelial PAS Domain-Containing Protein 1
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D000099162:Endothelial PAS Domain-Containing Protein 1
keywords: Cancer hypoxia; Cancer immunosuppression; ER; EpCAM; Forkhead-Box-Protein P3; FoxP3; HIF; HO-1; Human breast cancer; IDO-1; IL-10; Immune microenvironment; MDSC; NK cell; PPIA; PgR; Stat-3; Surgical specimen; TAM; TBP; TGF-β; TIL; Tata-box binding protein; Treg cells; VEGF; epithelial cell adhesion molecule; estrogen receptor; heme oxygenase-1; hypoxia inducible factor; indoleamine 2,3-dioxygenase; interleukin 10; myeloid-derived suppressor cells; natural killer cell; peptidylprolyl isomerase A=cyclophilin A; progesterone receptor; regulatory T cells; signal transducer and activator of transcription 3; transforming growth factor beta; tumour associated macrophage; tumour infiltration leukocytes; vascular endothelial growth factor