title: Isolation and identification of algicidal compound from Streptomyces and algicidal mechanism to Microcystis aeruginosa.
abstract: The biological control of cyanobacterial harmful algal blooms (cyanoHABs) is important to promote human health, environmental protection, and economic growth. Active algicidal compounds and algicidal mechanisms should be identified and investigated to control cyanoHABs. In this study, the algicidal actinobacterium Streptomyces sp. L74 was isolated from the soil of a nearby pond which located in the center lake of Guanghzou Higher Education Mega Center. Results showed that the algicidal activities of cyanoHABs are mainly achieved via an indirect attack by producing algicidal compounds. All active algicidal compounds are hydrophilic substances that are heat and pH stable. In the present study, an active compound (B3) was isolated and purified by high-performance liquid chromatography and identified as a type of triterpenoid saponin (2-hydroxy-12-oleanene-3, 28-O-D-glucopyranosyl) with a molecular formula of C42H70O13 as determined by infrared spectrometry, electrospray ionization mass spectrometry, and nuclear magnetic resonance. Active algicidal compounds from Streptomyces sp. L74 were shown to disrupt the antioxidant systems of Microcystis aeruginosa cells.
authors.affiliation: College of Bioscience and Bioengineering, South China University of Technology, Guangzhou, Pr China.
authors.forename: Jianfei
authors.initials: J
authors.lastname: Luo
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Shuishui
authors.initials: S
authors.lastname: Tang
authors.forename: Jianwen
authors.initials: J
authors.lastname: Liang
authors.forename: Weitie
authors.initials: W
authors.lastname: Lin
authors.forename: Lixin
authors.initials: L
authors.lastname: Luo
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000898:Antibiosis / Q000502:physiology; D057097:Harmful Algal Bloom / Q000187:drug effects*; D025301:Hyphae; D046931:Microcystis / Q000187:drug effects* / Q000254:growth & development; D010802:Phylogeny; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013171:Spores, Bacterial; D013302:Streptomyces / Q000145:classification / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S