title: Microspectroscopic Study of Liposome-to-cell Interaction Revealed by Förster Resonance Energy Transfer.
abstract: We report the Förster resonance energy transfer (FRET)-labeling of liposomal vesicles as an effective approach to study in dynamics the interaction of liposomes with living cells of different types (rat hepatocytes, rat bone marrow, mouse fibroblast-like cells and human breast cancer cells) and cell organelles (hepatocyte nuclei). The in vitro experiments were performed using fluorescent microspectroscopic technique. Two fluorescent dyes (DiO as the energy donor and DiI as an acceptor) were preloaded in lipid bilayers of phosphatidylcholine liposomes that ensures the necessary distance between the dyes for effective FRET. The change in time of the donor and acceptor relative fluorescence intensities was used to visualize and trace the liposome-to-cell interaction. We show that FRET-labeling of liposome vesicles allows one to reveal the differences in efficiency and dynamics of these interactions, which are associated with composition, fluidity, and metabolic activity of cell plasma membranes.
authors.affiliation: Institute for Scintillation Materials, NAS of Ukraine, 60 Lenin ave, 61001, Kharkov, Ukraine. ephimova@isma.kharkov.ua.
authors.forename: Svetlana L
authors.initials: SL
authors.lastname: Yefimova
authors.affiliation: Slavyansk State Pedagogical University, 19 Batyuka str, Slavyansk, 84116, Ukraine.
authors.forename: Irina Yu
authors.initials: IY
authors.lastname: Kurilchenko
authors.affiliation: Institute for Scintillation Materials, NAS of Ukraine, 60 Lenin ave, 61001, Kharkov, Ukraine.
authors.forename: Tatyana N
authors.initials: TN
authors.lastname: Tkacheva
authors.affiliation: Institute for Scintillation Materials, NAS of Ukraine, 60 Lenin ave, 61001, Kharkov, Ukraine.
authors.forename: Nataliya S
authors.initials: NS
authors.lastname: Kavok
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, 03022, Ukraine.
authors.forename: Igor N
authors.initials: IN
authors.lastname: Todor
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, 03022, Ukraine.
authors.forename: Nataliya Yu
authors.initials: NY
authors.lastname: Lukianova
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, 03022, Ukraine.
authors.forename: Vasyl F
authors.initials: VF
authors.lastname: Chekhun
authors.affiliation: Institute for Scintillation Materials, NAS of Ukraine, 60 Lenin ave, 61001, Kharkov, Ukraine.
authors.forename: Yuriy V
authors.initials: YV
authors.lastname: Malyukin
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006801:Humans; D008081:Liposomes; D051379:Mice; D051381:Rats
chemical_list: D008081:Liposomes
keywords: Fluorescent dyes; Förster resonance energy transfer; Liposome; Living cells