title: Spectrophotometric detection of histidine and lysine using combined enzymatic reactions.
abstract: An amino acid-sensing system with absorption spectrophotometric detection was developed. To ensure specific recognition of each amino acid, aminoacyl-tRNA synthetases were employed and the concentration of NADH produced by way of several enzymatic reactions was measured. Using this detection system, from 1.5 to 55 μM of histidine and from 15 to 95 μM of lysine could be measured selectively in HEPES-KOH buffer (pH 8.0).
authors.affiliation: Center for Industry and Public Relations, Hiroshima City University, 3-8-24 Senda-machi, Naka-ku, Hiroshima 730-0052, Japan. Electronic address: kugimiya@hiroshima-cu.ac.jp.
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Kugimiya
authors.forename: Emi
authors.initials: E
authors.lastname: Takamitsu
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D006639:Histidine / Q000032:analysis*; D006643:Histidine-tRNA Ligase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000032:analysis*; D008250:Lysine-tRNA Ligase / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D013053:Spectrophotometry
chemical_list: D009243:NAD; D006639:Histidine; D000604:Amino Acyl-tRNA Synthetases; D006643:Histidine-tRNA Ligase; D008250:Lysine-tRNA Ligase; D008239:Lysine
keywords: Amino acid; Aminoacyl-tRNA synthetase; Biosensing; Clinical examination; Food industry; β-Nicotinamide adenine dinucleotide