title: ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis.
abstract: In order to obtain insights into the regulatory pathways controlling phloem development, we characterized three genes encoding membrane proteins from the G sub-family of ABC transporters (ABCG9, ABCG11 and ABCG14), whose expression in the phloem has been confirmed. Mutations in the genes encoding these dimerizing 'half transporters' are semi-dominant and result in vascular patterning defects in cotyledons and the floral stem. Co-immunoprecipitation and bimolecular fluorescence complementation experiments demonstrated that these proteins dimerize, either by flexible pairing (ABCG11 and ABCG9) or by forming strict heterodimers (ABCG14). In addition, metabolome analyses and measurement of sterol ester contents in the mutants suggested that ABCG9, ABCG11 and ABCG14 are involved in lipid/sterol homeostasis regulation. Our results show that these three ABCG genes are required for proper vascular development in Arabidopsis thaliana.
authors.affiliation: Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-90187, Umeå, Sweden; Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, S-90183, Umeå, Sweden; UMR 1318, AgroParisTech, Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique Centre de Versailles, RD10, 78026, Versailles Cedex, France.
authors.forename: Rozenn
authors.initials: R
authors.lastname: Le Hir
authors.forename: Clément
authors.initials: C
authors.lastname: Sorin
authors.forename: Dipankar
authors.initials: D
authors.lastname: Chakraborti
authors.forename: Thomas
authors.initials: T
authors.lastname: Moritz
authors.forename: Hubert
authors.initials: H
authors.lastname: Schaller
authors.forename: Frédérique
authors.initials: F
authors.lastname: Tellier
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Robert
authors.forename: Halima
authors.initials: H
authors.lastname: Morin
authors.forename: Laszlo
authors.initials: L
authors.lastname: Bako
authors.forename: Catherine
authors.initials: C
authors.lastname: Bellini
mesh_terms: D000070996:ATP Binding Cassette Transporter, Subfamily G; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018548:Cotyledon / Q000235:genetics / Q000254:growth & development; D006706:Homeostasis; D055442:Metabolome; D009154:Mutation; D052585:Phloem / Q000235:genetics / Q000254:growth & development*; D010840:Phytosterols / Q000737:chemistry; D018547:Plant Stems / Q000235:genetics / Q000254:growth & development; D055503:Protein Multimerization
chemical_list: C525930:ABCG11 protein, Arabidopsis; C000589386:ABCG14 protein, Arabidopsis; C000592969:ABCG9 protein, Arabidopsis; D000070996:ATP Binding Cassette Transporter, Subfamily G; D018528:ATP-Binding Cassette Transporters; D029681:Arabidopsis Proteins; D010840:Phytosterols
keywords: ABC transporters; ABCG dimer; Arabidopsis thaliana; lipid/sterol homeostasis; vascular development