title: Proteomic approach for identification of immunogenic proteins of Mycoplasma mycoides subsp. capri.
abstract: In this study, an immunoproteomic approach was used to identify immunodominant proteins from Mycoplasma mycoides subsp. capri isolates. Membrane proteins, extracted through TX-114 phase partitioning, were separated using mono- and two-dimensional electrophoresis and detected by Western blotting with pooled sera from naturally infected goats. A total of 27 immunoreactive spots, corresponding to 13 different proteins, were identified using nanoLC-ESI-MSMS. Function annotation revealed that most of these proteins were metabolic enzymes involved in carbohydrate and energy metabolism. The immunogenic proteins identified in this study: pyruvate dehydrogenase, dihydrolipoamide acetyltransferase, dihydrolipoyl dehydrogenase, phosphate acetyltransferase, phosphopyruvate hydratase, adenine phopshoribosyltransferase, transketolase, translation elongation factor G, translation elongation factor Ts, FMN-dependent NADH-azoreductase, peptide methionine sulfoxide reductase, inorganic diphosphatase and trigger factor may be used as biomarkers for the serological diagnosis of contagious agalactia caused by M. mycoides subsp. capri.
authors.affiliation: Istituto Zooprofilattico Sperimentale della Sardegna "G. Pegreffi", 07100 Sassari, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Corona
authors.forename: G
authors.initials: G
authors.lastname: Cillara
authors.forename: S
authors.initials: S
authors.lastname: Tola
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004798:Enzymes / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D006041:Goats; D007106:Immune Sera / Q000378:metabolism; D015151:Immunoblotting; D009176:Mycoplasma mycoides / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011002:Pleuropneumonia, Contagious / Q000175:diagnosis; D040901:Proteomics
chemical_list: D001426:Bacterial Proteins; D004798:Enzymes; D007106:Immune Sera
keywords: 2-DE; Immunoblot; Immunoproteomics; Mass spectrometry; Mycoplasma mycoides subsp. capri