title: [Prokaryotic expression and immunogenicity analysis of phosphoglycerate kinase 1 of Candida albicans].
abstract: OBJECTIVE
  To construct prokaryotic expression plasmids of Candida albicans gene phosphoglycerate kinase 1 (pgk-1) and examine the immunogenicity of the recombinant protein.
  
  
  METHODS
  The full-length coding sequence of pgk-1 was amplified by PCR and cloned into the prokaryotic expression vector pET30a. The 6×His-tagged protein was induced by IPTG in E.coli BL-21(DE3) and the recombinant protein was identified by SDS-PAGE and Western blotting. The BALB/c mice were immunized with the purified recombinant protein to evaluate the antigenicity of the recombinant protein by ELISA.
  
  
  RESULTS
  The full-length pgk-1 gene was cloned from SC5314 genome and pET-30a-pgk-1 was successfully constructed. The recombinant protein PGK-1 was highly expressed in E.coli with a relative molecule mass of 54 810. ELISA indicated that the titer of the antibody was about 1:1024.
  
  
  CONCLUSIONS
  PGK-1 was successfully expressed by prokaryotic expression system and the recombinant protein showed favorable immunogenicity in mice.
authors.affiliation: Department of Clinical Laboratory, Bethune International Peace Hospital of PLA, Shijiazhuang 050082, China.
authors.forename: Zhengxin
authors.initials: Z
authors.lastname: He
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Ran
authors.forename: Xing
authors.initials: X
authors.lastname: Chen
authors.forename: Yun
authors.initials: Y
authors.lastname: Bai
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Xie
authors.forename: Tianwen
authors.initials: T
authors.lastname: Hou
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000201:enzymology* / Q000235:genetics; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D010735:Phosphoglycerate Kinase / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification
chemical_list: D007074:Immunoglobulin G; D011994:Recombinant Proteins; D010735:Phosphoglycerate Kinase