title: High-resolution structure of an atypical α-phosphoglucomutase related to eukaryotic phosphomannomutases.
abstract: The first structure of a bacterial α-phosphoglucomutase with an overall fold similar to eukaryotic phosphomannomutases is reported. Unlike most α-phosphoglucomutases within the α-D-phosphohexomutase superfamily, it belongs to subclass IIb of the haloacid dehalogenase superfamily (HADSF). It catalyzes the reversible conversion of α-glucose 1-phosphate to glucose 6-phosphate. The crystal structure of α-phosphoglucomutase from Lactococcus lactis (APGM) was determined at 1.5 Å resolution and contains a sulfate and a glycerol bound at the enzyme active site that partially mimic the substrate. A dimeric form of APGM is present in the crystal and in solution, an arrangement that may be functionally relevant. The catalytic mechanism of APGM and its strict specificity towards α-glucose 1-phosphate are discussed.
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa (ITQB-UNL), 2780-157 Oeiras, Portugal.
authors.forename: Przemyslaw
authors.initials: P
authors.lastname: Nogly
authors.forename: Pedro M
authors.initials: PM
authors.lastname: Matias
authors.forename: Matteo
authors.initials: M
authors.lastname: de Rosa
authors.forename: Rute
authors.initials: R
authors.lastname: Castro
authors.forename: Helena
authors.initials: H
authors.lastname: Santos
authors.forename: Ana Rute
authors.initials: AR
authors.lastname: Neves
authors.forename: Margarida
authors.initials: M
authors.lastname: Archer
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D020134:Catalytic Domain / Q000235:genetics; D018360:Crystallography, X-Ray; D019298:Glucose-6-Phosphate / Q000737:chemistry / Q000235:genetics; D005958:Glucosephosphates / Q000737:chemistry / Q000235:genetics; D006867:Hydrolases / Q000737:chemistry / Q000145:classification / Q000235:genetics; D013294:Lactococcus lactis / Q000201:enzymology* / Q000235:genetics; D018716:Molecular Mimicry / Q000235:genetics; D005810:Multigene Family; D017875:Phosphotransferases (Phosphomutases) / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D011485:Protein Binding / Q000235:genetics; D013379:Substrate Specificity / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D005958:Glucosephosphates; D019298:Glucose-6-Phosphate; C031590:glucose-1-phosphate; D006867:Hydrolases; C034507:2-haloacid dehalogenase; D017875:Phosphotransferases (Phosphomutases); C052799:phosphomannomutase
keywords: Lactococcus lactis; eukaryotic phosphomannomutases; haloacid dehalogenase superfamily; phosphomannomutases; sugar metabolism; α-glucose 1-phosphate; α-phosphoglucomutases