title: Induced resistance to Botrytis cinerea in Capsicum annuum by a Fusarium crude elicitor fraction, free of proteins.
abstract: Fusarium oxysporum f. sp. lycopersici (FOL) induces resistance in pepper against the airborne pathogen Botrytis cinerea and the soil-borne pathogen Verticillium dahliae. However, its practical use is limited due to its pathogenicity to other crops. In this study we tested several fractions of a heat-sterilised crude FOL-elicitor preparation to protect pepper against B. cinerea and V. dahliae. Only the protein-free insoluble fraction of the preparation reduced B. cinerea infection. However, none of the fractions reduce V. dahliae symptoms. The insoluble protein-free fraction induced expression of defence genes in the plant, namely a chitinase (CACHI2), a peroxidase (CAPO1), a sesquiterpene cyclase (CASC1) and a basic PR1 (CABPR1). Even though the CASC1 gene was not induced directly after treatment with the insoluble fraction in the leaves, it was induced after B. cinerea inoculation, showing a priming effect. The insoluble protein-free FOL-elicitor protected pepper against the airborne pathogen through a mechanism that involves induced responses in the plant, but different to the living FOL.
authors.affiliation: Depto. de Bioloxía Animal, Bioloxía Vexetal e Ecoloxía, Universidade da Coruña, Coruña, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Veloso
authors.forename: J
authors.initials: J
authors.lastname: Díaz
mesh_terms: D020171:Botrytis / Q000472:pathogenicity; D002212:Capsicum / Q000187:drug effects / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D002457:Cell Extracts / Q000737:chemistry / Q000494:pharmacology*; D005670:Fusarium / Q000737:chemistry*; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D018547:Plant Stems / Q000187:drug effects / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D013172:Spores, Fungal; D020172:Verticillium / Q000472:pathogenicity
chemical_list: D002457:Cell Extracts; D010940:Plant Proteins; C053376:pathogenesis-related proteins, plant
keywords: Fusarium oxysporum f. sp. lycopersici; PR proteins; Verticillium dahliae; induced resistance; saccharidic elicitors