title: Application of proteomics to identify the target molecules involved in Lonicera japonica-induced photokilling in human lung cancer CH27 cells.
abstract: BACKGROUND
  The Lonicera japonica has been used as natural and healthy drink for its anti-inflammatory effect and pleasant odor in China and Taiwan.
  
  
  METHODS
  2D electrophoresis was used to analyze the proteins involved in photoactivated Lonicera japonica-induced CH27 cell apoptosis. The fluorescent dyes MitoTracker Red CMXRos, calcein AM and JC-1 were used to elucidate mitochondrial function. The protein expression was performed by Western blotting. Fluorescent image of endoplasmic reticulum was accomplished by using ER-Tracker Green. This study used fluorescent dye CM-H2DCFDA to detect intracellular generation of reactive oxygen species.
  
  
  RESULTS
  The identified proteins can be classified into three major groups, which include proteins involved in mitochondrial function, cytoskeleton-related proteins and proteins associated with endoplasmic reticulum (ER) stress. Photoactivated Lonicera japonica caused a significant effect on the mitochondrial function and ER stress in CH27 cells. The reactive oxygen species producing was found to be involved in photoactivated Lonicera japonica-induced CH27 cell apoptosis.
  
  
  CONCLUSIONS
  Mitochondria and endoplasmic reticulum are the integral targets in photoactivated Lonicera japonica-induced CH27 cell apoptosis. We also demonstrated that ethyl acetate fraction of Lonicera japonica extracts caused photocytotoxicity in a dose-dependent manner in CH27 cells. This could explain the fact that the ethyl acetate fraction of Lonicera japonica extracts may contain compounds which exhibit the photosensitizing activity in CH27 cells.
authors.affiliation: School of Pharmacy, China Medical University, Taichung, Taiwan. hong@mail.cmu.edu.tw.
authors.forename: Jung C
authors.initials: JC
authors.lastname: Liao
authors.forename: Wen T
authors.initials: WT
authors.lastname: Chang
authors.forename: Yu H
authors.initials: YH
authors.lastname: Lan
authors.forename: Mann J
authors.initials: MJ
authors.lastname: Hour
authors.forename: Hong Z
authors.initials: HZ
authors.lastname: Lee
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects; D006801:Humans; D031239:Lonicera / Q000737:chemistry*; D008175:Lung Neoplasms / Q000737:chemistry / Q000188:drug therapy* / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects; D018832:Molecular Chaperones / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D055668:Photochemical Processes; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D020543:Proteome / Q000187:drug effects*; D040901:Proteomics; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D018832:Molecular Chaperones; D010936:Plant Extracts; D020543:Proteome; D017382:Reactive Oxygen Species