title: Power assisted liposuction to obtain adipose-derived stem cells: impact on viability and differentiation to adipocytes in comparison to manual aspiration.
abstract: BACKGROUND
  Adipose-derived stem cells (ASCs) play a key role in tissue engineering approaches and are probably of major importance in the context of autologous fat transfer. A number of different tools for harvesting ASCs-containing fat tissue have been established. Such devices should be easy to handle, time saving, low priced, safe and provide a high amount of viable ASCs in the aspirate. Power-assisted liposuction (PAL) has not yet been described in the literature as a tool for fat harvesting for lipotransfer. Aim of this study was to investigate ASCs' viability in fat tissue harvested using PAL versus manual aspiration (MA).
  
  
  METHODS
  Fat tissue was obtained from 9 donors undergoing abdominoplasty. Samples were divided into two sections. Out of each section fat was harvested using either PAL or MA. Number of isolated ASCs was defined, proliferation rate was determined and cell viability was assessed by flow cytometry. The ability of isolated ASCs to differentiate into mature adipocytes was analyzed by gene marker expression.
  
  
  RESULTS
  The number of viable ASCs and the proliferation rates did not significantly differ between PAL and MA but cells harvested using PAL showed significantly higher expression levels of differentiation markers adiponectin, GLUT4 and PPARg.
  
  
  CONCLUSIONS
  Our results show that PAL is a feasible method for harvesting fat tissue containing viable ASCs. Quantity and quality of PAL-harvested ASC is similar or even better, respectively, compared to ASCs harvested by MA.
authors.affiliation: Division of Plastic and Reconstructive Surgery, Department of Surgery, and Clinical Division of Endocrinology and Metabolism, Department of Medicine III, Medical University Vienna, Währingergürtel 18-22, 1090 Vienna, Austria. Electronic address: maike.keck@meduniwien.ac.at.
authors.forename: Maike
authors.initials: M
authors.lastname: Keck
authors.affiliation: Division of Plastic and Reconstructive Surgery, Department of Surgery, and Clinical Division of Endocrinology and Metabolism, Department of Medicine III, Medical University Vienna, Währingergürtel 18-22, 1090 Vienna, Austria.
authors.forename: Johanna
authors.initials: J
authors.lastname: Kober
authors.affiliation: Division of Plastic and Reconstructive Surgery, Department of Surgery, and Clinical Division of Endocrinology and Metabolism, Department of Medicine III, Medical University Vienna, Währingergürtel 18-22, 1090 Vienna, Austria.
authors.forename: Otto
authors.initials: O
authors.lastname: Riedl
authors.affiliation: Division of Plastic and Reconstructive Surgery, Department of Surgery, and Clinical Division of Endocrinology and Metabolism, Department of Medicine III, Medical University Vienna, Währingergürtel 18-22, 1090 Vienna, Austria.
authors.forename: Hugo B
authors.initials: HB
authors.lastname: Kitzinger
authors.affiliation: Division of Plastic and Reconstructive Surgery, Department of Surgery, and Clinical Division of Endocrinology and Metabolism, Department of Medicine III, Medical University Vienna, Währingergürtel 18-22, 1090 Vienna, Austria.
authors.forename: Sonja
authors.initials: S
authors.lastname: Wolf
authors.affiliation: Christian Doppler-Laboratory for Cardio-Metabolic Immunotherapy, Clinical Division of Endocrinology and Metabolism, Department of Medicine III, Medical University Vienna, Vienna, Austria.
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Stulnig
authors.affiliation: Christian Doppler-Laboratory for Cardio-Metabolic Immunotherapy, Clinical Division of Endocrinology and Metabolism, Department of Medicine III, Medical University Vienna, Vienna, Austria.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Zeyda
authors.affiliation: Division of Plastic and Reconstructive Surgery, Department of Surgery, and Clinical Division of Endocrinology and Metabolism, Department of Medicine III, Medical University Vienna, Währingergürtel 18-22, 1090 Vienna, Austria.
authors.forename: Alfred
authors.initials: A
authors.lastname: Gugerell
mesh_terms: D017667:Adipocytes / Q000166:cytology; D052242:Adiponectin / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D015870:Gene Expression; D051275:Glucose Transporter Type 4 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D015187:Lipectomy / Q000379:methods*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D020858:Tissue and Organ Harvesting / Q000379:methods*
chemical_list: D052242:Adiponectin; D051275:Glucose Transporter Type 4; D047495:PPAR gamma; D012333:RNA, Messenger; C495692:SLC2A4 protein, human
keywords: Adipocytes; Adiponectin; Autologous fat transplantation; Centrifugation; Differentiation; GLUT4; PPARƴ