title: Effect of multiple freeze and thaw cycles on the sensitivity of IgG and IgM immunoglobulins in the sera of patients with syphilis.
abstract: We describe the effects of multiple freeze and thaw cycles on the sensitivity of the immunoglobulins IgG and IgM measured by enzyme-linked immunoassays in the sera of patients with syphilis. Stored frozen sera can withstand repeated freezing and thawing cycles with a minimal detrimental effect on the sensitivity of the sera.
authors.affiliation: From the Division of STD Prevention, Centers for Disease Control and Prevention, Atlanta, GA.
authors.forename: Arnold R
authors.initials: AR
authors.lastname: Castro
authors.forename: Heather A
authors.initials: HA
authors.lastname: Jost
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D055550:Protein Stability; D015203:Reproducibility of Results; D013588:Syphilis Serodiagnosis / Q000379:methods; D014021:Tissue Preservation / Q000379:methods*; D014210:Treponema pallidum / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M