title: Identification of a novel human testicular interstitial gene, RNF148, and its expression regulated by histone deacetylases.
abstract: Multiple genes are restrictively expressed in mammalian testicular tissues, and they play important roles in the complex process of spermatogenesis. Investigation of these genes and their expression regulation mechanisms is valuable to elucidate the molecular process of spermatogenesis. In this study, we identified a novel human gene, ring finger protein 148 (RNF148) that is abundantly expressed in testes and slightly expressed in pancreas. In situ hybridization analysis showed that RNF148 messenger RNA was mainly present in the interstitial cells of human testicular tissues, and immunohistochemical analysis confirmed protein levels in that location. Treatment with histone deacetylase inhibitor trichostatin A activated the expression of RNF148 messenger RNA in a time- and concentration-dependent manner in HEK293T and HeLa cells, neither of which normally express RNF148. Chromatin immunoprecipitation analysis showed that trichostatin A treatment increased the binding of acetylated histone H3 to the RNF148 gene promoter. We identified a novel human testicular interstitial gene and observed that histone deacetylases regulate RNF148 expression.
authors.affiliation: Department of Medical Genetics, Division of Human Morbid Genomics, State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, China yq_liu@scu.edu.cn.
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Liu
authors.forename: D C
authors.initials: DC
authors.lastname: Tao
authors.forename: S Y
authors.initials: SY
authors.lastname: Liao
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: Y X
authors.initials: YX
authors.lastname: Ma
authors.forename: S Z
authors.initials: SZ
authors.lastname: Zhang
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D002681:China; D047369:Chromatin Immunoprecipitation; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006367:HeLa Cells; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006655:Histone Deacetylases / Q000378:metabolism*; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D007985:Leydig Cells / Q000166:cytology* / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D054829:RING Finger Domains; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*
chemical_list: D056572:Histone Deacetylase Inhibitors; D006657:Histones; D006877:Hydroxamic Acids; D012333:RNA, Messenger; C012589:trichostatin A; D044767:Ubiquitin-Protein Ligases; D006655:Histone Deacetylases