title: Flavonoids from the leaves of Carya cathayensis Sarg. inhibit vascular endothelial growth factor-induced angiogenesis.
abstract: The total flavonoids (TFs) were isolated from the leaves of Carya cathayensis Sarg. (LCC), a well-known Chinese medicinal herb commercially cultivated in Tianmu Mountain district, a cross area of Zhejiang and Anhui provinces in China. Five flavonoids, i.e. cardamonin, pinostrobin chalcone (PC), wogonin, chrysin, and pinocembrin were the main components of the TFs. The TFs and these pure compounds suppressed vascular endothelial growth factor (VEGF)-induced angiogenesis as detected in the mouse aortic ring assay, and cardamonin showed the best effect among them. To further elucidate the mechanisms for suppressing angiogenesis of these flavonoids, assays of VEGF-induced proliferation and migration in human umbilical vein endothelial cells (HUVECs) were performed. The TFs, cardamonin, pinocembrin, and chrysin obviously suppressed both VEGF-induced HUVEC proliferation and migration. However, PC and wogonin not only slightly inhibited VEGF-induced proliferation but also remarkably suppressed those of migration in HUVECs. Our further study showed that cardamonin decreased the phosphorylation of ERK and AKT induced by VEGF with a dose-dependent manner in HUVECs. Our findings indicate that the TFs and these pure flavonoids may become potential preventive and/or therapeutic agents against angiogenesis-related diseases.
authors.affiliation: College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
authors.forename: Sha-Sha
authors.initials: SS
authors.lastname: Tian
authors.affiliation: College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
authors.forename: Fu-Sheng
authors.initials: FS
authors.lastname: Jiang
authors.affiliation: College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
authors.forename: Xue-Xin
authors.initials: XX
authors.lastname: Zhu
authors.affiliation: College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
authors.forename: Jin-Jian
authors.initials: JJ
authors.lastname: Lu
authors.affiliation: College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China. Electronic address: zjtcmdzs@163.com.
authors.forename: Zhi-Shan
authors.initials: ZS
authors.lastname: Ding
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D031325:Carya / Q000737:chemistry*; D002465:Cell Movement; D049109:Cell Proliferation; D047188:Chalcones / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D044950:Flavanones / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects*; D006801:Humans; D008297:Male; D051379:Mice; D018515:Plant Leaves / Q000737:chemistry; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D020533:Angiogenesis Inhibitors; D047188:Chalcones; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D042461:Vascular Endothelial Growth Factor A; C043561:chrysin; C016063:pinocembrin; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases; C436747:cardamonin; C085514:wogonin; C411294:pinostrobin
keywords: Angiogenesis; Flavonoids; Migration; Proliferation; VEGF