title: Quadruplex-forming sequences occupy discrete regions inside plant LTR retrotransposons.
abstract: Retrotransposons with long terminal repeats (LTR) form a significant proportion of eukaryotic genomes, especially in plants. They have gag and pol genes and several regulatory regions necessary for transcription and reverse transcription. We searched for potential quadruplex-forming sequences (PQSs) and potential triplex-forming sequences (PTSs) in 18 377 full-length LTR retrotransposons collected from 21 plant species. We found that PQSs were often located in LTRs, both upstream and downstream of promoters from which the whole retrotransposon is transcribed. Upstream-located guanine PQSs were dominant in the minus DNA strand, whereas downstream-located guanine PQSs prevailed in the plus strand, indicating their role both at transcriptional and post-transcriptional levels. Our circular dichroism spectroscopy measurements confirmed that these PQSs readily adopted guanine quadruplex structures-some of them were paralell-stranded, while others were anti-parallel-stranded. The PQS often formed doublets at a mutual distance of up to 400 bp. PTSs were most abundant in 3'UTR (but were also present in 5'UTR). We discuss the potential role of quadruplexes and triplexes as the regulators of various processes participating in LTR retrotransposon life cycle and as potential recombination sites during post-insertional retrotransposon-based genome rearrangements.
authors.affiliation: Department of Information Technologies, Faculty of Informatics, Masaryk University, Botanicka 68a, 60200 Brno, Czech Republic, Laboratory of Genome Dynamics, CEITEC - Central European Institute of Technology, Masaryk University, Zerotinovo nam 9, 60177 Brno, Czech Republic, Department of Plant Developmental Genetics, Institute of Biophysics ASCR, Kralovopolska 135, 61265 Brno, Czech Republic, Laboratory of CD Spectroscopy, CEITEC - Central European Institute of Technology, Masaryk University, Zerotinovo nam 9, 60177 Brno, Czech Republic and Department of CD Spectroscopy of Nucleic Acids, Institute of Biophysics ASCR, Kralovopolska 135, 61265 Brno, Czech Republic.
authors.forename: Matej
authors.initials: M
authors.lastname: Lexa
authors.forename: Eduard
authors.initials: E
authors.lastname: Kejnovský
authors.forename: Pavlína
authors.initials: P
authors.lastname: Steflová
authors.forename: Helena
authors.initials: H
authors.lastname: Konvalinová
authors.forename: Michaela
authors.initials: M
authors.lastname: Vorlícková
authors.forename: Boris
authors.initials: B
authors.lastname: Vyskot
mesh_terms: D018744:DNA, Plant / Q000737:chemistry*; D054856:G-Quadruplexes; D018745:Genome, Plant; D018626:Retroelements; D017422:Sequence Analysis, DNA; D020079:Terminal Repeat Sequences
chemical_list: D018744:DNA, Plant; D018626:Retroelements