title: Antibacterial activity of Nymphaea nouchali (Burm. f) flower.
abstract: BACKGROUND
  The present work aimed to find out the antibacterial activity of Nymphaea nouchali flower on human and plant pathogenic bacteria.
  
  
  METHODS
  Antibacterial potency of methanol, acetone, ethyl acetate and petroleum spirit extracts of Nymphaea nouchali flower has been tested against four human pathogenic bacteria Bacillus subtilis (FO 3026) Escherichia coli (IFO 3007), Klebsiella pneumonia (ATTC 10031) and Sarcina lutea (IFO 3232) and one plant pathogenic bacterium Xanthomonas campestris (IAM 1671) by disc diffusion assay. Zone of inhibition produced by different extracts against the test bacteria was measured and compared with standard antibiotic disc.
  
  
  RESULTS
  Methanol extract possessed better antibacterial activity against two pathogenic bacteria, B. subtilis (FO 3026) and S. lutea (IFO 3232) than commercial antibiotic nalidixic acid. Acetone extract showed moderate sensitivity whereas B. subtilis (FO 3026), S. lutea (IFO 3232) and X. campestris (IAM 1671) showed resistance to ethyl acetate and petroleum spirit extracts. The minimum inhibitory concentrations of various extracts were ranged between 128-2048 μgml-1.
  
  
  CONCLUSIONS
  Nymphaea nouchali flower could be a potential candidate for future development of novel broad spectrum antibacterial herbal formulation.
authors.affiliation: Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia 7003, Bangladesh. jamalbtg@gmail.com.
authors.forename: Biplab Kumar
authors.initials: BK
authors.lastname: Dash
authors.forename: Monokesh Kumer
authors.initials: MK
authors.lastname: Sen
authors.forename: Khasrul
authors.initials: K
authors.lastname: Alam
authors.forename: Kamal
authors.initials: K
authors.lastname: Hossain
authors.forename: Rezuanul
authors.initials: R
authors.lastname: Islam
authors.forename: Nilufa Akhter
authors.initials: NA
authors.lastname: Banu
authors.forename: Shahedur
authors.initials: S
authors.lastname: Rahman
authors.forename: Abu Hena Mostofa
authors.initials: AH
authors.lastname: Jamal
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D009268:Nalidixic Acid / Q000494:pharmacology; D030721:Nymphaea / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D012505:Sarcina / Q000187:drug effects; D016959:Xanthomonas campestris / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D009268:Nalidixic Acid