title: Generation and characterization of a rabbit monoclonal antibody site-specific for tau O-GlcNAcylated at serine 400.
abstract: Aggregation of tau into paired helical filaments is a pathological process leading to neurotoxicity in Alzheimer's disease and other tauopathies. Tau is posttranslationally modified by O-linked N-acetylglucosamine (O-GlcNAc), and increasing tau O-GlcNAcylation may protect against its aggregation. Research tools to study the relationship between tau aggregation and tau O-GlcNAcylation have not been widely available. Here we describe the generation of a rabbit monoclonal antibody specific for tau O-GlcNAcylated at Ser400 (O-tau(S400)). We show the utility of this antibody for in vitro and in vivo experiments to investigate the function of O-GlcNAc modifications of tau at Ser400.
authors.affiliation: EMD Serono Research & Development Institute, Billerica, MA 01821, United States.
authors.forename: Andrew
authors.initials: A
authors.lastname: Cameron
authors.forename: Brandy
authors.initials: B
authors.lastname: Giacomozzi
authors.forename: John
authors.initials: J
authors.lastname: Joyce
authors.forename: Audrey
authors.initials: A
authors.lastname: Gray
authors.forename: Danielle
authors.initials: D
authors.lastname: Graham
authors.forename: Solenne
authors.initials: S
authors.lastname: Ousson
authors.forename: Maud
authors.initials: M
authors.lastname: Neny
authors.forename: Dirk
authors.initials: D
authors.lastname: Beher
authors.forename: George
authors.initials: G
authors.lastname: Carlson
authors.forename: Jill
authors.initials: J
authors.lastname: O'Moore
authors.forename: Mark
authors.initials: M
authors.lastname: Shearman
authors.forename: Heike
authors.initials: H
authors.lastname: Hering
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000918:Antibody Specificity; D006031:Glycosylation; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D011817:Rabbits; D012694:Serine / Q000378:metabolism; D016875:tau Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D016875:tau Proteins; D012694:Serine; D000117:Acetylglucosamine
keywords: Monoclonal antibody; O-GlcNAc; OGA; Site-specific; Tau