title: Exercise ameliorates renal cell apoptosis in chronic kidney disease by intervening in the intrinsic and the extrinsic apoptotic pathways in a rat model.
abstract: We hypothesized that doxorubicin (DR) induced chronic kidney disease (CKD) could trigger the intrinsic and the extrinsic renal cell apoptotic pathways, while treadmill exercise could help prevent adverse effects. Male Sprague-Dawley rats were subjected to treadmill running exercise at a speed of 30 m/min, 30 or 60 min/day, 3 times per week, for a total period of 11 weeks. The physiological and biochemical parameters were seen substantially improved (DR-CKD control, 30 min, 60 min exercise): the ratio of kidney weight/body weight (0.89, 0.74, and 0.72); the WBC (1.35, 1.08, and 1.42 × 10(4) cells/ μ L); RBC (5.30, 6.38, and 6.26 × 10(6) cells/ μ L); the platelet count (15.1, 12.8, and 11.3 × 10(5)/ μ L); serum cholesterol (659, 360, and 75 mg/dL); serum triglyceride (542, 263, and 211 mg/dL); BUN (37, 25, and 22 mg/dL). Bcl-2 and intramitochondrial cytochrome c were upregulated, while the levels of Bax, SOD, MDA, cleaved caspases 9, 3, 8, 12, and calpain were all downregulated in DRCKD groups with exercise. CHOP (GADD153) and GRP78 were totally unaffected. FAS (CD95) was only slightly suppressed in the 60 min exercise DRCKD group. Conclusively, exercise can ameliorate CKD through the regulation of the intrinsic and extrinsic apoptosis pathways. The 60 min exercise yields more beneficial effect than the 30 min counterpart.
authors.affiliation: Department of Urology, School of Medicine, College of Medicine, Taipei Medical University, 250 Wu-Shing Street, Taipei 11031, Taiwan ; Department of Urology, Taipei Medical University-Shuang Ho Hospital, Taipei Medical University, 291 Zhongzheng Road, Zhonghe, Taipei 23561, Taiwan.
authors.forename: Kuan-Chou
authors.initials: KC
authors.lastname: Chen
authors.forename: Chiung-Chi
authors.initials: CC
authors.lastname: Peng
authors.forename: Chiu-Lan
authors.initials: CL
authors.lastname: Hsieh
authors.forename: Robert Y
authors.initials: RY
authors.lastname: Peng