title: Pyranonigrin E: a PKS-NRPS hybrid metabolite from Aspergillus niger identified by genome mining.
abstract: Induced production of PKS-NRPS metabolites: the genome mining approach is useful for obtaining new compounds. We activated a dormant PKS-NRPS gene cluster in Aspergillus niger ATCC 1015 by expressing its dedicated transcriptional activator (PynR). As a result, the transformant expressing pynR produced a new pyranonigrin compound that we have named pyranonigirin E.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku Tokyo 113-0033 (Japan).
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Awakawa
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Yang
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Wakimoto
authors.forename: Ikuro
authors.initials: I
authors.lastname: Abe
mesh_terms: D001234:Aspergillus niger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D057225:Data Mining; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D016681:Genome, Fungal; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D005810:Multigene Family; D010453:Peptide Synthases / Q000235:genetics* / Q000378:metabolism; D048630:Polyketide Synthases / Q000235:genetics* / Q000378:metabolism; D011753:Pyrones / Q000737:chemistry / Q000378:metabolism*; D011758:Pyrroles / Q000737:chemistry / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D011753:Pyrones; D011758:Pyrroles; D015534:Trans-Activators; C587979:pyranonigrin E; D048630:Polyketide Synthases; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase
keywords: gene expression; genome mining; peptides; polyketides; transcriptional factors