title: Giga-pixel fluorescent imaging over an ultra-large field-of-view using a flatbed scanner.
abstract: We demonstrate a new fluorescent imaging technique that can screen for fluorescent micro-objects over an ultra-wide field-of-view (FOV) of ~532 cm(2), i.e., 19 cm × 28 cm, reaching a space-bandwidth product of more than 2 billion. For achieving such a large FOV, we modified the hardware and software of a commercially available flatbed scanner, and added a custom-designed absorbing fluorescent filter, a two-dimensional array of external light sources for computer-controlled and high-angle fluorescent excitation. We also re-programmed the driver of the scanner to take full control of the scanner hardware and achieve the highest possible exposure time, gain and sensitivity for detection of fluorescent micro-objects through the gradient index self-focusing lens array that is positioned in front of the scanner sensor chip. For example, this large FOV of our imaging platform allows us to screen more than 2.2 mL of undiluted whole blood for detection of fluorescent micro-objects within <5 minutes. This high-throughput fluorescent imaging platform could be useful for rare cell research and cytometry applications by enabling rapid screening of large volumes of optically dense media. Our results constitute the first time that a flatbed scanner has been converted to a fluorescent imaging system, achieving a record large FOV.
authors.affiliation: Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA. ozcan@ucla.edu.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Göröcs
authors.forename: Yuye
authors.initials: Y
authors.lastname: Ling
authors.forename: Meng Dai
authors.initials: MD
authors.lastname: Yu
authors.forename: Dimitri
authors.initials: D
authors.lastname: Karahalios
authors.forename: Kian
authors.initials: K
authors.lastname: Mogharabi
authors.forename: Kenny
authors.initials: K
authors.lastname: Lu
authors.forename: Qingshan
authors.initials: Q
authors.lastname: Wei
authors.forename: Aydogan
authors.initials: A
authors.lastname: Ozcan
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D012984:Software
chemical_list: D005456:Fluorescent Dyes