title: Mitochondrial DNA polymorphism in genes encoding ND1, COI and CYTB in canine malignant cancers.
abstract: The aim of the study was to identify DNA changes in mitochondrial gene fragments: NADH dehydrogenase subunit 1 (ND1), cytochrome c oxidase subunit I (COI) and cytochrome b (CYTB) in tumor tissue, normal tissue and blood, and to define their association with the tumor type in dogs. Molecular analysis included 144 tests in total. A functional effect of the non-synonymous protein coding SNP was predicted. The presence of polymorphisms in all tested gene fragments in individual tissues of dogs was observed. Heteroplasmic changes were found in ND1 and CYTB in epithelioma glandulae sebacei and in CYTB in lymphoma centroblasticum. The results of in silico analysis show the impact of these alleles (COI: 507, ND1: 450, 216, CYTB: 748) on the functioning of proteins and thus their potential role in carcinogenesis. The possible harmful effects of changes in polypeptides in positions T193N, V98M, V118M and H196P were evaluated. It seems that polymorphisms occurring in cells can have a negative impact on functioning of proteins. This promotes disorders of the energy level in cells.
authors.affiliation: Department of Biological Bases of Animal Production, University of Life Sciences in Lublin , Lublin , Poland .
authors.forename: Brygida
authors.initials: B
authors.lastname: Slaska
authors.forename: Ludmila
authors.initials: L
authors.lastname: Grzybowska-Szatkowska
authors.forename: Sylwia
authors.initials: S
authors.lastname: Nisztuk
authors.forename: Magdalena
authors.initials: M
authors.lastname: Surdyka
authors.forename: Dorota
authors.initials: D
authors.lastname: Rozanska
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002277:Carcinoma / Q000235:genetics / Q000473:pathology / Q000662:veterinary; D045303:Cytochromes b / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000302:isolation & purification; D004285:Dogs; D003576:Electron Transport Complex IV / Q000235:genetics*; D005260:Female; D008223:Lymphoma / Q000235:genetics / Q000473:pathology / Q000662:veterinary; D008297:Male; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics*; D009369:Neoplasms / Q000235:genetics / Q000473:pathology* / Q000662:veterinary; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D045303:Cytochromes b; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV
keywords: Cancer; dog; genes; heteroplasmy; mtDNA