title: Structural characterization and antioxidant activity of a low-molecular polysaccharide from Dendrobium huoshanense.
abstract: The present study aimed at investigating the structural features and antioxidant activities of a polysaccharide fraction (DHP1A) obtained from Dendrobium huoshanense, a precious herb medicine in China. DHP1A mainly consisted of mannose (Man), glucose (Glc) and a trace of galactose (Gal), with a molecular weight of 6700Da. Its backbone contained (1→4)-linked α-D-Glcp, (1→6)-linked α-D-Glcp and (1→4)-linked β-D-Manp, with a branch of terminal β-D-Galp. The in vitro antioxidant evaluation revealed that DHP1A had a remarkable inhibition effect on the FeCl2-induced lipid peroxidation. Furthermore, DHP1A pretreatment decreased the production of malondialdehyde (MDA), and restored the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), as well as the level of glutathione (GSH) in the livers of CCl4-treated mice. These results suggested that DHP1A was a potential antioxidant component in D. huoshanense.
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, PR China.
authors.forename: Chang-Cheng
authors.initials: CC
authors.lastname: Tian
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, PR China.
authors.forename: Xue-Qiang
authors.initials: XQ
authors.lastname: Zha
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, PR China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Pan
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, PR China. Electronic address: jianpingluo@hfut.edu.cn.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Luo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D002251:Carbon Tetrachloride; D002374:Catalase / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy / Q000201:enzymology*; D031667:Dendrobium / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D015394:Molecular Structure; D008970:Molecular Weight; D010084:Oxidation-Reduction; D008517:Phytotherapy; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Antioxidant activity; Dendrobium huoshanense; Polysaccharide; Structure