title: Computational analysis of the soluble form of the intracellular chloride ion channel protein CLIC1.
abstract: The chloride intracellular channel (CLIC) family of proteins has the remarkable property of maintaining both a soluble form and an integral membrane form acting as an ion channel. The soluble form is structurally related to the glutathione-S-transferase family, and CLIC can covalently bind glutathione via an active site cysteine. We report approximately 0.6 μs of molecular dynamics simulations, encompassing the three possible ligand-bound states of CLIC1, using the structure of GSH-bound human CLIC1. Noncovalently bound GSH was rapidly released from the protein, whereas the covalently ligand-bound protein remained close to the starting structure over 0.25 μs of simulation. In the unliganded state, conformational changes in the vicinity of the glutathione-binding site resulted in reduced reactivity of the active site thiol. Elastic network analysis indicated that the changes in the unliganded state are intrinsic to the protein architecture and likely represent functional transitions. Overall, our results are consistent with a model of CLIC function in which covalent binding of glutathione does not occur spontaneously but requires interaction with another protein to stabilise the GSH binding site and/or transfer of the ligand. The results do not indicate how CLIC1 undergoes a radical conformational change to form a transmembrane chloride channel but further elucidate the mechanism by which CLICs are redox controlled.
authors.affiliation: School of Medical and Molecular Biosciences, University of Technology Sydney, P.O. Box 123, Broadway, NSW 2007, Australia.
authors.forename: Peter M
authors.initials: PM
authors.lastname: Jones
authors.forename: Paul M G
authors.initials: PM
authors.lastname: Curmi
authors.forename: Stella M
authors.initials: SM
authors.lastname: Valenzuela
authors.forename: Anthony M
authors.initials: AM
authors.lastname: George
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D020134:Catalytic Domain; D018118:Chloride Channels / Q000737:chemistry*; D002712:Chlorides / Q000737:chemistry*; D019295:Computational Biology / Q000379:methods*; D018360:Crystallography, X-Ray; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008024:Ligands; D011485:Protein Binding; D011487:Protein Conformation
chemical_list: C501966:CLIC1 protein, human; D018118:Chloride Channels; D002712:Chlorides; D008024:Ligands; D005978:Glutathione; D003545:Cysteine