title: Effects of aflatoxin B1 on oxidative stress markers and apoptosis of spleens in broilers.
abstract: The purpose of the present study was to investigate the oxidative damage and apoptosis induced by aflatoxin B1 (AFB1) in spleen of broilers. A total of 200 one-day-old avian male broilers were randomly divided into 4 equal groups of 50 each and were fed for 21 days as follows: a control diet and three AFB1 diets containing 0.15, 0.3, and 0.6 mg AFB1/kg diet. Consumption of AFB1 diets induced oxidative stress in the spleen of chicken as evidenced by reduced glutathione peroxidase, glutathione reductase, and catalase activities, decreased glutathione contents, and increased malondialdehyde contents in explaining the pathogenesis. Flow cytometer method and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling assay revealed that the apoptotic splenocytes were increased in AFB1 groups. The results suggest that AFB1 induced excessive apoptosis of splenic lymphocytes, which is correlated with increased oxidative stress. The present results may be helpful for explaining the pathogenesis of AFB1-induced immunosuppression.
authors.affiliation: Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, People's Republic of China.
authors.forename: Kejie
authors.initials: K
authors.lastname: Chen
authors.affiliation: Institute of Rare Animals and Plants, College of Life Sciences, China West Normal University, Nanchong, Sichuan, People's Republic of China.
authors.forename: Shibin
authors.initials: S
authors.lastname: Yuan
authors.affiliation: Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, People's Republic of China pengxi197313@163.com.
authors.forename: Xi
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
authors.affiliation: Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, People's Republic of China.
authors.forename: Fengyuan
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, People's Republic of China.
authors.forename: Hengmin
authors.initials: H
authors.lastname: Cui
authors.affiliation: Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, People's Republic of China.
authors.forename: Zhengli
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, People's Republic of China.
authors.forename: Jingxin
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Geng
mesh_terms: D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002645:Chickens; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D007165:Immunosuppression Therapy; D020287:In Situ Nick-End Labeling; D015227:Lipid Peroxidation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011897:Random Allocation; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D017304:Annexin A5; D015415:Biomarkers; D008315:Malondialdehyde; D016604:Aflatoxin B1; D002374:Catalase; D005980:Glutathione Reductase; D005978:Glutathione
keywords: Aflatoxin B1; apoptosis; flow cytometry; oxidative stress; spleen