title: Influence of the CCR-5/MIP-1 α axis in the pathogenesis of Rocio virus encephalitis in a mouse model.
abstract: Rocio virus (ROCV) caused an outbreak of human encephalitis during the 1970s in Brazil and its immunopathogenesis remains poorly understood. CC-chemokine receptor 5 (CCR5) is a chemokine receptor that binds to macrophage inflammatory protein (MIP-1 α). Both molecules are associated with inflammatory cells migration during infections. In this study, we demonstrated the importance of the CCR5 and MIP-1 α, in the outcome of viral encephalitis of ROCV-infected mice. CCR5 and MIP-1 α knockout mice survived longer than wild-type (WT) ROCV-infected animals. In addition, knockout mice had reduced inflammation in the brain. Assessment of brain viral load showed mice virus detection five days post-infection in wild-type and CCR5-/- mice, while MIP-1 α-/- mice had lower viral loads seven days post-infection. Knockout mice required a higher lethal dose than wild-type mice as well. The CCR5/MIP-1 α axis may contribute to migration of infected cells to the brain and consequently affect the pathogenesis during ROCV infection.
authors.affiliation: Laboratório de Virologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais; Centro de Pesquisa em Virologia, Departamento de Bioquímica e Imunologia da Faculdade de Medicina Departamento de Patologia de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil; Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil; Departamento de Microbiologia e Parasitologia e Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Rio Grande do Norte, Natal, Brazil.
authors.forename: Juliana H
authors.initials: JH
authors.lastname: Chávez
authors.forename: Rafael F O
authors.initials: RF
authors.lastname: França
authors.forename: Carlo J F
authors.initials: CJ
authors.lastname: Oliveira
authors.forename: Maria T P
authors.initials: MT
authors.lastname: de Aquino
authors.forename: Kleber J S
authors.initials: KJ
authors.lastname: Farias
authors.forename: Paula R L
authors.initials: PR
authors.lastname: Machado
authors.forename: Thelma F M
authors.initials: TF
authors.lastname: de Oliveira
authors.forename: Jonny
authors.initials: J
authors.lastname: Yokosawa
authors.forename: Edson G
authors.initials: EG
authors.lastname: Soares
authors.forename: João S
authors.initials: JS
authors.lastname: da Silva
authors.forename: Benedito A L
authors.initials: BA
authors.lastname: da Fonseca
authors.forename: Luiz T M
authors.initials: LT
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D002465:Cell Movement; D054405:Chemokine CCL3 / Q000172:deficiency / Q000235:genetics*; D018792:Encephalitis, Viral / Q000378:metabolism* / Q000401:mortality / Q000473:pathology / Q000821:virology; D005416:Flavivirus / Q000502:physiology*; D018177:Flavivirus Infections / Q000378:metabolism* / Q000401:mortality / Q000473:pathology / Q000821:virology; D015870:Gene Expression; D054884:Host-Pathogen Interactions; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000401:mortality / Q000473:pathology / Q000821:virology; D008214:Lymphocytes / Q000378:metabolism / Q000473:pathology / Q000821:virology; D008264:Macrophages / Q000378:metabolism / Q000473:pathology / Q000821:virology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011485:Protein Binding; D019713:Receptors, CCR5 / Q000172:deficiency / Q000235:genetics*; D015398:Signal Transduction; D016019:Survival Analysis; D019562:Viral Load
chemical_list: C496534:Ccl3 protein, mouse; D054405:Chemokine CCL3; D019713:Receptors, CCR5