title: Competition enhances stochasticity in biochemical reactions.
abstract: We study stochastic dynamics of two competing complexation reactions (i) A + B↔AB and (ii) A + C↔AC. Such reactions are common in biology where different reactants compete for common resources--examples range from binding enzyme kinetics to gene expression. On the other hand, stochasticity is inherent in biological systems due to small copy numbers. We investigate the complex interplay between competition and stochasticity, using coupled complexation reactions as the model system. Within the master equation formalism, we compute the exact distribution of the number of complexes to analyze equilibrium fluctuations of several observables. Our study reveals that the presence of competition offered by one reaction (say A + C↔AC) can significantly enhance the fluctuation in the other (A + B↔AB). We provide detailed quantitative estimates of this enhanced fluctuation for different combinations of rate constants and numbers of reactant molecules that are typical in biology. We notice that fluctuations can be significant even when two of the reactant molecules (say B and C) are infinite in number, maintaining a fixed stoichiometry, while the other reactant (A) is finite. This is purely due to the coupling mediated via resource sharing and is in stark contrast to the single reaction scenario, where large numbers of one of the components ensure zero fluctuation. Our detailed analysis further highlights regions where numerical estimates of mass action solutions can differ from the actual averages. These observations indicate that averages can be a poor representation of the system, hence analysis that is purely based on averages such as mass action laws can be potentially misleading in such noisy biological systems. We believe that the exhaustive study presented here will provide qualitative and quantitative insights into the role of noise and its enhancement in the presence of competition that will be relevant in many biological settings.
authors.affiliation: Department of Physics and Astronomy, University of Denver, Denver, Colorado 80208, USA.
authors.forename: Taylor
authors.initials: T
authors.lastname: Firman
authors.forename: Kingshuk
authors.initials: K
authors.lastname: Ghosh
mesh_terms: D004798:Enzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D013269:Stochastic Processes; D013997:Time Factors
chemical_list: D004798:Enzymes