title: Web-based visualisation and analysis of 3D electron-microscopy data from EMDB and PDB.
abstract: The Protein Data Bank in Europe (PDBe) has developed web-based tools for the visualisation and analysis of 3D electron microscopy (3DEM) structures in the Electron Microscopy Data Bank (EMDB) and Protein Data Bank (PDB). The tools include: (1) a volume viewer for 3D visualisation of maps, tomograms and models, (2) a slice viewer for inspecting 2D slices of tomographic reconstructions, and (3) visual analysis pages to facilitate analysis and validation of maps, tomograms and models. These tools were designed to help non-experts and experts alike to get some insight into the content and assess the quality of 3DEM structures in EMDB and PDB without the need to install specialised software or to download large amounts of data from these archives. The technical challenges encountered in developing these tools, as well as the more general considerations when making archived data available to the user community through a web interface, are discussed.
authors.affiliation: Protein Data Bank in Europe, European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SD, United Kingdom. Electronic address: ingvar@ebi.ac.uk.
authors.forename: Ingvar
authors.initials: I
authors.lastname: Lagerstedt
authors.forename: William J
authors.initials: WJ
authors.lastname: Moore
authors.forename: Ardan
authors.initials: A
authors.lastname: Patwardhan
authors.forename: Eduardo
authors.initials: E
authors.lastname: Sanz-García
authors.forename: Christoph
authors.initials: C
authors.lastname: Best
authors.forename: Jason R
authors.initials: JR
authors.lastname: Swedlow
authors.forename: Gerard J
authors.initials: GJ
authors.lastname: Kleywegt
mesh_terms: D030562:Databases, Protein; D055032:Electron Microscope Tomography; D021621:Imaging, Three-Dimensional; D020407:Internet; D008958:Models, Molecular; D020836:Protein Structure, Quaternary; D011506:Proteins / Q000737:chemistry / Q000648:ultrastructure; D012984:Software
chemical_list: D011506:Proteins
keywords: 3DEM; EM VTC; EMDB; Electron Microscopy Data Bank; Electron Microscopy Validation Task Force; Electron microscopy; Electron tomography; FSC; Fourier Shell Correlation; Macromolecular structure; OAV; OME; OME Remote Objects; OMERO; Open Astex Viewer; Open Microscopy Environment; PDB; PDBe; Protein Data Bank; Protein Data Bank in Europe; Three-Dimensional Electron Microscopy; Visualisation; Worldwide Protein Data Bank; wwPDB