title: Effects of combined radiofrequency radiation exposure on levels of reactive oxygen species in neuronal cells.
abstract: The objective of this study was to investigate the effects of the combined RF radiation (837 MHz CDMA plus 1950 MHz WCDMA) signal on levels of intracellular reactive oxygen species (ROS) in neuronal cells. Exposure of the combined RF signal was conducted at specific absorption rate values of 2 W/kg of CDMA plus 2 W/kg of WCDMA for 2 h. Co-exposure to combined RF radiation with either H2O2 or menadione was also performed. The experimental exposure groups were incubator control, sham-exposed, combined RF radiation-exposed with or without either H2O2 or menadione groups. The intracellular ROS level was measured by flow cytometry using the fluorescent probe dichlorofluorescein diacetate. Intracellular ROS levels were not consistently affected by combined RF radiation exposure alone in a time-dependent manner in U87, PC12 or SH-SY5Y cells. In neuronal cells exposed to combined RF radiation with either H2O2 or menadione, intracellular ROS levels showed no statically significant alteration compared with exposure to menadione or H2O2 alone. These findings indicate that neither combined RF radiation alone nor combined RF radiation with menadione or H2O2 influences the intracellular ROS level in neuronal cells such as U87, PC12 or SH-SY5Y.
authors.affiliation: Research Center for Radio-senescence, and Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences, Seoul 139-706, Korea.
authors.forename: Kyoung Ah
authors.initials: KA
authors.lastname: Kang
authors.forename: Hyung Chul
authors.initials: HC
authors.lastname: Lee
authors.forename: Je-Jung
authors.initials: JJ
authors.lastname: Lee
authors.forename: Mi-Na
authors.initials: MN
authors.lastname: Hong
authors.forename: Myung-Jin
authors.initials: MJ
authors.lastname: Park
authors.forename: Yun-Sil
authors.initials: YS
authors.lastname: Lee
authors.forename: Hyung-Do
authors.initials: HD
authors.lastname: Choi
authors.forename: Nam
authors.initials: N
authors.lastname: Kim
authors.forename: Young-Gyu
authors.initials: YG
authors.lastname: Ko
authors.forename: Jae-Seon
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D051379:Mice; D041681:NIH 3T3 Cells; D009474:Neurons / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology*; D011829:Radiation Dosage; D011836:Radiation Tolerance / Q000502:physiology* / Q000528:radiation effects; D011846:Radio Waves; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D017382:Reactive Oxygen Species
keywords: H2O2; combined RF radiation; menadione; neuronal cells; reactive oxygen species