title: Portable device for magnetic stimulation: assessment survival and proliferation in human lymphocytes.
abstract: A device's instrumentation for magnetic stimulation on human lymphocytes is presented. This is a new procedure to stimulate growing cells with ferrofluid in vortices of magnetic field. The stimulation of magnetic vortices was provided at five different frequencies, from 100 to 2500 Hz and intensities from 1.13 to 4.13 mT. To improve the stimulation effects, a paramagnetic ferrofluid was added on the cell culture medium. The results suggest that the frequency changes and the magnetic field variation produce an important increase in the number of proliferating cells as well as in the cellular viability. This new magnetic stimulation modality could trigger an intracellular mechanism to induce cell proliferation and cellular survival only on mitogen stimulated cells.
authors.affiliation: Department of Physical Engineering - DCI, Universidad de Guanajuato campus León, Loma del Bosque 103, Lomas del Campestre, 37150 León, GTO, Mexico.
authors.forename: H
authors.initials: H
authors.lastname: Pérez
authors.forename: T
authors.initials: T
authors.lastname: Cordova-Fraga
authors.forename: S
authors.initials: S
authors.lastname: López-Briones
authors.forename: J C
authors.initials: JC
authors.lastname: Martínez-Espinosa
authors.forename: E F
authors.initials: EF
authors.lastname: Rosas
authors.forename: A
authors.initials: A
authors.lastname: Espinoza
authors.forename: J C
authors.initials: JC
authors.lastname: Villagómez-Castro
authors.forename: M
authors.initials: M
authors.lastname: Sosa
authors.forename: S
authors.initials: S
authors.lastname: Topsu
authors.forename: J J
authors.initials: JJ
authors.lastname: Bernal-Alvarado
mesh_terms: D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D060526:Magnetic Fields; D008934:Mitogens / Q000494:pharmacology
chemical_list: D003470:Culture Media; D008934:Mitogens