title: Extracellular Hsp72 concentration relates to a minimum endogenous criteria during acute exercise-heat exposure.
abstract: Extracellular heat shock protein 72 (eHsp72) concentration increases during exercise-heat stress when conditions elicit physiological strain. Differences in severity of environmental and exercise stimuli have elicited varied response to stress. The present study aimed to quantify the extent of increased eHsp72 with increased exogenous heat stress, and determine related endogenous markers of strain in an exercise-heat model. Ten males cycled for 90 min at 50 % [Formula: see text] in three conditions (TEMP, 20 °C/63 % RH; HOT, 30.2 °C/51%RH; VHOT, 40.0 °C/37%RH). Plasma was analysed for eHsp72 pre, immediately post and 24-h post each trial utilising a commercially available ELISA. Increased eHsp72 concentration was observed post VHOT trial (+172.4 %) (p < 0.05), but not TEMP (-1.9 %) or HOT (+25.7 %) conditions. eHsp72 returned to baseline values within 24 h in all conditions. Changes were observed in rectal temperature (Trec), rate of Trec increase, area under the curve for Trec of 38.5 and 39.0 °C, duration Trec ≥38.5 and ≥39.0 °C, and change in muscle temperature, between VHOT, and TEMP and HOT, but not between TEMP and HOT. Each condition also elicited significantly increasing physiological strain, described by sweat rate, heart rate, physiological strain index, rating of perceived exertion and thermal sensation. Stepwise multiple regression reported rate of Trec increase and change in Trec to be predictors of increased eHsp72 concentration. Data suggests eHsp72 concentration increases once systemic temperature and sympathetic activity exceeds a minimum endogenous criteria elicited during VHOT conditions and is likely to be modulated by large, rapid changes in core temperature.
authors.affiliation: School of Sport and Service Management, Welkin Science Laboratories, University of Brighton, 30 Carlisle Road, Eastbourne, UK, o.r.gibson@brighton.ac.uk.
authors.forename: Oliver R
authors.initials: OR
authors.lastname: Gibson
authors.forename: Alex
authors.initials: A
authors.lastname: Dennis
authors.forename: Tony
authors.initials: T
authors.lastname: Parfitt
authors.forename: Lee
authors.initials: L
authors.lastname: Taylor
authors.forename: Peter W
authors.initials: PW
authors.lastname: Watt
authors.forename: Neil S
authors.initials: NS
authors.lastname: Maxwell
mesh_terms: D001831:Body Temperature; D015444:Exercise; D005110:Extracellular Space / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000097:blood*; D006339:Heart Rate / Q000502:physiology; D006358:Hot Temperature; D006801:Humans; D008297:Male; D013312:Stress, Physiological; D055815:Young Adult
chemical_list: D018840:HSP70 Heat-Shock Proteins; C490786:HSPA1A protein, human