title: Injectable calcium phosphate-alginate-chitosan microencapsulated MC3T3-E1 cell paste for bone tissue engineering in vivo.
abstract: Osteoblasts or stem cells have been delivered into injectable calcium phosphate cement (CPC) to improve its effectiveness and biological function. However, the osteogenic potential of the new construct in vivo has been rarely reported, and there are no reports on alginate-chitosan microencapsulated osteoblasts mixed with CPC. This study aimed to develop alginate-chitosan microencapsulated mouse osteoblast MC3T3-E1 cells (AC-cells), evaluate the osteogenic potential of a calcium phosphate cement complex with these AC-cells (CPC-AC-cell), and trace the implanted MC3T3-E1 cells in vivo. MC3T3-E1 cells were embedded in alginate microcapsules, cultured in osteogenic medium for 7 days, and then covered with chitosan before mixing with a paste of β-tricalcium phosphate/calcium phosphate cement (β-TCP/CPC). The construct was injected into the dorsal subcutaneous area of nude mice. Lamellar-bone-like mineralization, newly formed collagen and angiogenesis were observed at 4 weeks. At 8 weeks, areas of newly formed collagen expanded; further absorption of β-TCP/CPC and osteoid-like structures could be seen. Cell tracing in vivo showed that implanted MC3T3-E1 cells were clearly visible at 2 weeks. These in vivo results indicate that the novel injectable CPC-AC-cell construct is promising for bone tissue engineering applications.
authors.affiliation: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Pengyan
authors.initials: P
authors.lastname: Qiao
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Qiufei
authors.initials: Q
authors.lastname: Xie
authors.forename: Fangfang
authors.initials: F
authors.lastname: Li
authors.forename: Limin
authors.initials: L
authors.lastname: Dong
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001843:Bone Cements / Q000737:chemistry; D016025:Bone Transplantation; D001842:Bone and Bones / Q000473:pathology; D002130:Calcium Phosphates / Q000737:chemistry*; D002214:Capsules / Q000737:chemistry; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D000464:Alginates; D001843:Bone Cements; D002130:Calcium Phosphates; D002214:Capsules; D006603:Hexuronic Acids; C485817:beta-tricalcium phosphate; D020723:Glucuronic Acid; D048271:Chitosan; C020243:calcium phosphate
keywords: Calcium phosphate cement; Chitosan; In vivo; Injectable scaffold; Microencapsulation; Osteoblast