title: Investigation of ACE genome insertion/deletion correlation with immunohistochemical profile in pituitary adenomas.
abstract: OBJECTIVE
  The deletion polymorphism of the angiotensin-converting enzyme (ACE) genome causes neoplastic development in several organs by increasing the angiotensin 2 (A2) formation. In this study, we aimed to identify the ACE genome insertion/deletion polymorphism in pituitary adenomas and to compare it with the control group.
  
  
  METHODS
  Patients operated for pituitary adenomas were included in the study. Genomic DNA was extracted from tumoral tissues and peripheral blood samples of the patients by using the Miller method. Primary sequence was selected via targeting the polymorphic region of intron 16 of ACE genome 17q23. DNA samples were multiplied by PCR using HACE3s and HACE3as primers.
  
  
  RESULTS
  Twenty-one operated cases were studied. In the study group; 44 % of the patients were identified as D/D, 33% of them as I/D and 23% of them as I/I. In 60%, D allele was identified. According to immunohistochemical investigation, we found that 100% of the patients with Cushing adenoma were D/D alleles.
  
  
  CONCLUSIONS
  Presence of high rate of ACE genome deletion in patients with pituitary adenoma and grade 3-4 patients suggest that ACE genome polymorphism can be a risk factor for the development of pituitary adenomas.
authors.affiliation: Mustafa Kemal University, Faculty of Medicine, Department of Neurosurgery, Antakya, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Altas
authors.forename: Omer Faruk
authors.initials: OF
authors.lastname: Bayrak
authors.forename: Alpay
authors.initials: A
authors.lastname: Serefhan
authors.forename: Gokalp
authors.initials: G
authors.lastname: Silav
authors.forename: Kerim Kenan
authors.initials: KK
authors.lastname: Coskun
authors.forename: Ajlan
authors.initials: A
authors.lastname: Cerci
authors.forename: Nejat
authors.initials: N
authors.lastname: Isik
authors.forename: Ilhan
authors.initials: I
authors.lastname: Elmaci
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D002648:Child; D002847:Chromatography, Agarose; D004247:DNA / Q000235:genetics; D005260:Female; D017353:Gene Deletion; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D016254:Mutagenesis, Insertional / Q000235:genetics*; D019635:Neurosurgical Procedures; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D010911:Pituitary Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000601:surgery; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012307:Risk Factors; D055815:Young Adult
chemical_list: D004247:DNA; D007703:Peptidyl-Dipeptidase A