title: Expression of α-taxilin in the murine gastrointestinal tract: potential implication in cell proliferation.
abstract: α-Taxilin, a binding partner of the syntaxin family, is a candidate tumor marker. To gain insight into the physiological role of α-taxilin in normal tissues, we examined α-taxilin expression by Western blot and performed immunochemical analysis in the murine gastrointestinal tract where cell renewal vigorously occurs. α-Taxilin was expressed in the majority of the gastrointestinal tract and was prominently expressed in epithelial cells positive for Ki-67, a marker of actively proliferating cells. In the small intestine, α-taxilin was expressed in transient-amplifying cells and crypt base columnar cells intercalated among Paneth cells. In the corpus and antrum of the stomach, α-taxilin was expressed in cells localized in the lower pit and at the gland, respectively, but not in parietal or zymogenic cells. During development of the small intestine, α-taxilin was expressed in Ki-67-positive regions. Inhibition of cell proliferation by suppression of the Notch cascade using a γ-secretase inhibitor led to a decrease in α-taxilin- and Ki-67-positive cells in the stomach. These results suggest that expression of α-taxilin is regulated in parallel with cell proliferation in the murine gastrointestinal tract.
authors.affiliation: Department of Molecular and Cell Biology, Graduate School of Medicine, Dokkyo Medical University, 880 Kitakobayashi, Mibu-cho, Tochigi, 321-0293, Japan.
authors.forename: Yukimi
authors.initials: Y
authors.lastname: Horii
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakane
authors.forename: Satoru
authors.initials: S
authors.lastname: Nogami
authors.forename: Natsuko
authors.initials: N
authors.lastname: Ohtomo
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Tomiya
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Shirataki
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D041981:Gastrointestinal Tract / Q000166:cytology* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D041681:NIH 3T3 Cells; D033921:Vesicular Transport Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D033921:Vesicular Transport Proteins