title: Determination of vitamin D and its metabolites.
abstract: The demand for analysis of 25-hydroxyvitamin D has increased dramatically throughout the world over the past decade. As a consequence, a number of new automated assays have been introduced for 25-hydroxyvitamin D measurement. Automated assays have shown variable ability to meet the technical challenges associated with 25-hydroxyvitamin D measurement. Assays are able to meet performance goals for precision at high concentrations but fail to do so at low concentrations of 25-hydroxyvitamin D. The overall accuracy of automated methods has improved over recent years and generally shows good overall agreement with reference methods; however, discrepancies persist for individual samples. Liquid chromatography-tandem mass spectrometry is used by some routine laboratories for 25-hydroxyvitamin D analysis but its widespread use is hampered by limited sample throughput. 1,25-Dihydroxyvitamin D is an important analyte in specific clinical situations, which remains in the hands of specialised laboratories using manual analytical methods.
authors.affiliation: Laverty Pathology, 60 Waterloo Rd, North Ryde, NSW 2113, Australia.
authors.forename: Christopher-John
authors.initials: CJ
authors.lastname: Farrell
authors.forename: Markus
authors.initials: M
authors.lastname: Herrmann
mesh_terms: D000328:Adult; D001322:Autoanalysis; D002762:Cholecalciferol / Q000096:biosynthesis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D006801:Humans; D006900:Hydroxylation; D007223:Infant; D011485:Protein Binding; D011863:Radioimmunoassay; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism
chemical_list: D014807:Vitamin D; D002762:Cholecalciferol; C097949:1,25-dihydroxyvitamin D; C104450:25-hydroxyvitamin D
keywords: 25-hydroxyvitamin D; high-performance liquid chromatography; immunoassays; liquid chromatography-tandem mass spectrometry