title: Protein expression in silica dust-induced transdifferentiated rats lung fibroblasts.
abstract: OBJECTIVE
  To analyze the expression of different proteins in free silica-induced transdifferentiated rat lung fibroblasts.
  
  
  METHODS
  Rat lung fibroblasts and alveolar macrophages were cultured. A transdifferentiation model of rat lung fibroblasts was established. Free silica was used as a stimulator for rat lung fibroblasts. Changes in α-SMA were detected by immunohistochemistry and Western blot, respectively. Protein of lung fibroblasts was extracted and analyzed by two-dimensional electrophoresis (2-DE).
  
  
  RESULTS
  Six protein spots were identified by mass spectrometry, including glyceraldehyde 3-phosphate-dehydrogenase, peroxiredoxin 5, heterogeneous nuclear ribonucleoprotein A2, transgelin 2, keratin K6 and vimentin.
  
  
  CONCLUSIONS
  Some proteins are changed in free silica-induced transdifferentiated rat lung fibroblasts.
authors.affiliation: Department of Occupational Health and Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou 450001, Henan, China.
authors.forename: Chang Fu
authors.initials: CF
authors.lastname: Hao
authors.forename: Xiao Fang
authors.initials: XF
authors.lastname: Li
authors.forename: Wu
authors.initials: W
authors.lastname: Yao
mesh_terms: D000818:Animals; D054338:Cell Transdifferentiation; D015180:Electrophoresis, Gel, Two-Dimensional; D005347:Fibroblasts / Q000378:metabolism*; D016676:Macrophages, Alveolar / Q000502:physiology; D008297:Male; D051381:Rats; D012822:Silicon Dioxide; D012829:Silicosis / Q000209:etiology*
chemical_list: D012822:Silicon Dioxide
keywords: Crystalline silica; Myofibroblast; Transdifferentiation; Two-dimensional electrophoresis