title: Phytochemical, anti-oxidant and Anthelmintic activities of various leaf extracts of Flacourtia sepiaria Roxb.
abstract: OBJECTIVE
  The present study was carried out to investigate the phytochemical constituents, In vitro antioxidant potential and anthelmintic activities of Flacourtia sepiaria Roxb leaves.
  
  
  METHODS
  The dried powdered leaves of Flacourtia sepiaria were extracted using petroleum ether, chloroform, ethyl acetate and methanol by a soxhlet extractor and preliminary phytochemical screening was performed using standard protocols. All the extract was evaluated for their potential antioxidant activities using test such as DPPH, superoxide anion radical, hydroxyl radical, nitric oxide radical scavenging abilities, ferrous chelating ability and total phenolic and flavanoid content. Anthelmintic activity of extract was screened in adult Indian earthworm model.
  
  
  RESULTS
  Preliminary screening revealed the presence of bioactive compounds especially phenolics, tannins and terpenoids in all extracts. The phenolic and flavanoid content was highest in methanolic extract and lowest in petroleum ether extract. The paralytic (9.46±0.212) and death time (31.43±0.148) of methanolic extract was found to be significant (P<0.05) when compared with paralytic (7.33±0.206) and death time (18.60±0.229) of standard piperazine citrate at 100 mg/mL concentration.
  
  
  CONCLUSIONS
  The results of the present study indicate that the leaf extracts of Flacourtia sepiaria exhibited strong antioxidant activity and possess significant anthelmintic activity and thus it is a good source of antioxidant and anthelmintic constituents.
authors.affiliation: Department of Pharmaceutical Chemistry, National College of Pharmacy, Calicut, Kerala. Electronic address: aksreephd@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Sreejith
authors.forename: N
authors.initials: N
authors.lastname: Kannappan
authors.forename: A
authors.initials: A
authors.lastname: Santhiagu
authors.forename: Ajith P
authors.initials: AP
authors.lastname: Mathew
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000032:analysis / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D001681:Biological Assay; D006801:Humans; D009835:Oligochaeta / Q000187:drug effects; D064209:Phytochemicals / Q000737:chemistry* / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D031308:Salicaceae / Q000737:chemistry*; D016019:Survival Analysis
chemical_list: D000871:Anthelmintics; D000975:Antioxidants; D064209:Phytochemicals; D010936:Plant Extracts
keywords: Anthelmintic; Antioxidant; Flacourtia sepiaria; Piperazine citrate