title: Differential expression of heat shock proteins in healthy and diseased human renal allografts.
abstract: BACKGROUND
  Determining patterns of heat shock protein (Hsp) induction in normal kidneys and transplanted kidneys could provide insight into pathogenesis. The aim was to evaluate Hsp expression in normal kidneys, normal renal allografts and diseased renal allografts.
  
  
  METHODS
  Immunohistochemical staining for Hsp27, 60, 70i and 72/73 was performed on 11 morphologically normal human kidney nephrectomy specimens and needle biopsies of 32 human renal allografts.
  
  
  RESULTS
  Within the normal kidney, Hsp27 was detected in the endothelium, distal tubules and collecting ducts. Hsp60 was present within the distal convoluted tubules and ascending thick limb. Inducible Hsp70 (Hsp70i) was identified in the collecting ducts and ascending thick limb. Constitutively-expressed Hsp72/73 stained within podocytes and parietal epithelium. In renal allografts the low grade constitutive expression of Hsp in normal allografts became changed in disease. In acute cellular rejection and cyclophilin toxicity, distal tubules showed increased cytoplasmic immunopositivity for Hsp27, 60 and 70i. In chronic allograft nephropathy there was also induction of Hsp27, 60 and 70i in the distal tubules but this was less pronounced. Constitutively-expressed Hsp72/73 wasn't significantly induced in any diseased grafts.
  
  
  CONCLUSIONS
  Multiple Hsps are induced in diseased renal allografts, particularly within the distal nephron and the induction profile differs between diseases.
authors.affiliation: MRC Centre for Inflammation Research, Tissue Injury and Repair Group, Edinburgh, U.K.
authors.forename: Stephen
authors.initials: S
authors.lastname: O'Neill
authors.forename: Timothy G
authors.initials: TG
authors.lastname: Ingman
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Wigmore
authors.forename: Ewen M
authors.initials: EM
authors.lastname: Harrison
authors.forename: Christopher O
authors.initials: CO
authors.lastname: Bellamy
mesh_terms: D064591:Allografts / Q000378:metabolism*; D006084:Graft Rejection / Q000378:metabolism* / Q000473:pathology; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000378:metabolism* / Q000473:pathology
chemical_list: D006360:Heat-Shock Proteins