title: Different phosphorylation rates among vertebrate cone visual pigments with different spectral sensitivities.
abstract: Cone photoreceptor subtypes having different spectral sensitivities exhibit different recovery kinetics in their photoresponses in some vertebrates. Phosphorylation by G protein-coupled receptor kinase (GRK) is essential for the rapid inactivation of light-activated visual pigment, which is the rate-limiting step of the cone photoresponse recovery in salamander. In this study we compared the rate of light-dependent phosphorylation by GRK7 of carp green- and blue-sensitive cone visual pigments. Blue pigment was phosphorylated significantly less effectively than green pigment, suggesting that the difference in the pigment phosphorylation rate is responsible for the difference in photoresponse kinetics among cone photoreceptor subtypes.
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: tsutsui.kei.4c@kyoto-u.ac.jp.
authors.forename: Kei
authors.initials: K
authors.lastname: Tsutsui
authors.forename: Shuji
authors.initials: S
authors.lastname: Tachibanaki
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Shimauchi-Matsukawa
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Shichida
authors.forename: Satoru
authors.initials: S
authors.lastname: Kawamura
mesh_terms: D000818:Animals; D002347:Carps; D003001:Cloning, Molecular; D055358:Cone Opsins / Q000235:genetics / Q000378:metabolism*; D054768:G-Protein-Coupled Receptor Kinases / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D010766:Phosphorylation
chemical_list: D055358:Cone Opsins; D054768:G-Protein-Coupled Receptor Kinases
keywords: 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate; CHAPS; Cone visual pigment; G protein-coupled receptor kinase; GRK; PC; Phosphorylation; Pi; UV; absorption maximum wavelength; inorganic phosphate; l-α-phosphatidylcholine; ultraviolet; λ(max)