title: A nanosensor based on quantum-dot haptens for rapid, on-site immunoassay of cyanotoxin in environmental water.
abstract: A nanoprobe based on quantum-dot (QD) haptens was synthesized by conjugating carboxyl quantum dots with aminoethyl-microcystin (MC)-leucine-arginine (LR). A two-alkyl group was introduced to supply a spacer between the QD nanoprobe and anti-MC-LR antibody to reduce the steric hindrances of immunoreaction. The sensor system based on a portable optofluidic platform exhibited a liner range of 0.10-4.0 µg/L for MC-LR with a detection limit of 0.03 µg/L. The proposed sensor has potential application in the rapid, on-site detection of MC-LR in real water samples.
authors.affiliation: School of Environment and Natural Resources, Renmin University of China, Haidian, Beijing 100872, China; School of Environment, Tsinghua University, Haidian, Beijing 100084, China. Electronic address: longf04@ruc.edu.cn.
authors.forename: Long
authors.initials: L
authors.lastname: Feng
authors.forename: Anna
authors.initials: A
authors.lastname: Zhu
authors.forename: Hongchen
authors.initials: H
authors.lastname: Wang
authors.forename: Hanchang
authors.initials: H
authors.lastname: Shi
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D006241:Haptens / Q000737:chemistry; D007118:Immunoassay; D057230:Limit of Detection; D008387:Marine Toxins; D052998:Microcystins / Q000737:chemistry / Q000302:isolation & purification*; D015335:Molecular Probes / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
chemical_list: D006241:Haptens; D008387:Marine Toxins; D052998:Microcystins; D015335:Molecular Probes; D014874:Water Pollutants, Chemical; C057862:cyanoginosin LR
keywords: Cyanotoxin; Fluorescence resonance energy transfer; Nanosensor; Optofluidic; Quantum dot