title: Terriglobus tenax sp. nov., an exopolysaccharide-producing acidobacterium isolated from rhizosphere soil of a medicinal plant.
abstract: An exopolysaccharide-producing bacterium, designated strain DRP 35(T), was isolated from the rhizosphere soil of a medicinal herb, Angelica sinensis, at Geumsan in Korea. Cells were Gram-staining-negative, non-motile, catalase-positive and oxidase-negative short rods. The isolate grew aerobically from 15 to 45 °C (optimum 30 °C), pH 3.5-7.0 (optimum pH 5.0) and in the presence of 0-1.0% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain DRP 35(T) belongs to the genus Terriglobus in the phylum Acidobacteria with a sequence similarity of 97.2% and 97.0% to Terriglobus saanensis SP1PR4(T) and Terriglobus roseus KBS63(T), respectively. The genomic DNA G+C content was 62.1 mol%. DNA-DNA relatedness between strain DRP 35(T) and the type strains of the other species of the genus Terriglobus, T. saanensis SP1PR4(T) and T. roseus KBS63(T), were 24.6 and 17.2%, respectively. The predominant menaquinone was MK-8. Major fatty acids were iso-C(15 : 0), C(16 : 1)ω7c and C(16 : 0). The polar lipids were phosphatidylethanolamine, unidentified aminophospholipid and unknown phospholipids. On the basis of polyphasic analysis from this study, strain DRP 35(T) represents a novel species of the genus Terriglobus for which the name Terriglobus tenax sp. nov. is proposed. The type strain is DRP 35(T) ( = KACC 16474(T) = NBRC 109677(T)).
authors.affiliation: Institute of Microbial Ecology and Resources, Mokwon University, Doan-dong 800, Seo-gu, Daejeon 302-729, Republic of Korea.|Department of Microbial & Nano Materials, College of Science & Technology, Mokwon University, Doan-dong 800, Seo-gu, Daejeon 302-729, Republic of Korea.
authors.forename: Kyung-Sook
authors.initials: KS
authors.lastname: Whang
authors.affiliation: Institute of Microbial Ecology and Resources, Mokwon University, Doan-dong 800, Seo-gu, Daejeon 302-729, Republic of Korea.
authors.forename: Jae-Chan
authors.initials: JC
authors.lastname: Lee
authors.affiliation: Department of Microbial & Nano Materials, College of Science & Technology, Mokwon University, Doan-dong 800, Seo-gu, Daejeon 302-729, Republic of Korea.
authors.forename: Hae-Ran
authors.initials: HR
authors.lastname: Lee
authors.affiliation: Department of Microbial & Nano Materials, College of Science & Technology, Mokwon University, Doan-dong 800, Seo-gu, Daejeon 302-729, Republic of Korea.
authors.forename: Song-Ih
authors.initials: SI
authors.lastname: Han
authors.affiliation: Department of Microbial & Nano Materials, College of Science & Technology, Mokwon University, Doan-dong 800, Seo-gu, Daejeon 302-729, Republic of Korea.
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Chung
mesh_terms: D061271:Acidobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D029971:Angelica sinensis / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010946:Plants, Medicinal / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C024223:vitamin MK 8