title: Detection of canine pneumovirus in dogs with canine infectious respiratory disease.
abstract: Canine pneumovirus (CnPnV) was recently identified during a retrospective survey of kenneled dogs in the United States. In this study, archived samples from pet and kenneled dogs in the United Kingdom were screened for CnPnV to explore the relationship between exposure to CnPnV and the development of canine infectious respiratory disease (CIRD). Within the pet dog population, CnPnV-seropositive dogs were detected throughout the United Kingdom and Republic of Ireland, with an overall estimated seroprevalence of 50% (n = 314/625 dogs). In the kennel population, there was a significant increase in seroprevalence, from 26% (n = 56/215 dogs) on the day of entry to 93.5% (n = 201/215 dogs) after 21 days (P <0001). Dogs that were seronegative on entry but seroconverted while in the kennel were 4 times more likely to develop severe respiratory disease than those that did not seroconvert (P < 0.001), and dogs with preexisting antibodies to CnPnV on the day of entry were significantly less likely to develop respiratory disease than immunologically naive dogs (P < 0.001). CnPnV was detected in the tracheal tissues of 29/205 kenneled dogs. Detection was most frequent in dogs with mild to moderate respiratory signs and histopathological changes and in dogs housed for 8 to 14 days, which coincided with a significant increase in the risk of developing respiratory disease compared to the risk of those housed 1 to 7 days (P < 0.001). These findings demonstrate that CnPnV is present in the United Kingdom dog population; there is a strong association between exposure to CnPnV and CIRD in the kennel studied and a potential benefit in vaccinating against CnPnV as part of a wider disease prevention strategy.
authors.affiliation: Department of Pathology and Pathogen Biology, The Royal Veterinary College, North Mymms, Hatfield, Hertfordshire, United Kingdom.
authors.forename: Judy A
authors.initials: JA
authors.lastname: Mitchell
authors.forename: Jacqueline M
authors.initials: JM
authors.lastname: Cardwell
authors.forename: Randall W
authors.initials: RW
authors.lastname: Renshaw
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Dubovi
authors.forename: Joe
authors.initials: J
authors.lastname: Brownlie
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000453:epidemiology* / Q000821:virology*; D004285:Dogs; D007494:Ireland / Q000453:epidemiology; D057805:Pets; D018111:Pneumovirus / Q000276:immunology / Q000302:isolation & purification*; D018186:Pneumovirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D012141:Respiratory Tract Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D012189:Retrospective Studies; D016036:Seroepidemiologic Studies; D014132:Trachea / Q000821:virology; D006113:United Kingdom / Q000453:epidemiology