title: FT-Raman study of deferoxamine and deferiprone exhibits potent amelioration of structural changes in the liver tissues of mice due to aluminum exposure.
abstract: The present study inform the alterations on major biochemical constituents such as lipids, proteins, nucleic acids and glycogen along with phosphodiester linkages, tryptophan bands, tyrosine doublet, disulfide bridge conformations, aliphatic hydrophobic residue, and salt bridges in liver tissues of mice using Fourier transform Raman spectroscopy. In amide I, amide II and amide III, the area value significant decrease due structural alteration in the protein, glycogen and triglycerides levels but chelating agents DFP and DFO upturned it. Morphology changes by aluminium induced alterations and recovery by chelating agents within liver tissues known by histopathological examination. Concentrations of trace elements were found by ICP-OES. FT-Raman study was revealed to be in agreement with biochemical studies and demonstrate that it can successfully specify the molecular alteration in liver tissues. The tyrosyl doublet ratio I899/I831 decreases more in aluminum intoxicated tissues but treatment with DFP and DFO+DFP brings back to nearer control value. This indicates more variation in the hydrogen bonding of the phenolic hydroxyl group due to aluminum poisoning. The decreased Raman intensity ratio (I3220/I3400) observed in the aluminum induced tissues suggests a decreased water domain size, which could be interpreted in terms of weaker hydrogen-bonded molecular species of water in the aluminum intoxicated liver tissues. Finally, FT-Raman spectroscopy might be a useful tool for obtained successfully to indicate the molecular level changes.
authors.affiliation: Department of Physics, Annamalai University, Annamalai Nagar, Tamilnadu 608002, India. Electronic address: girihari777@yahoo.com.
authors.forename: S
authors.initials: S
authors.lastname: Sivakumar
authors.forename: Chandra Prasad
authors.initials: CP
authors.lastname: Khatiwada
authors.forename: J
authors.initials: J
authors.lastname: Sivasubramanian
authors.forename: B
authors.initials: B
authors.lastname: Raja
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D000818:Animals; D002614:Chelating Agents / Q000494:pharmacology*; D000077543:Deferiprone; D003676:Deferoxamine / Q000494:pharmacology*; D008099:Liver / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D011728:Pyridones / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D002614:Chelating Agents; D011728:Pyridones; D000077543:Deferiprone; D000535:Aluminum; D003676:Deferoxamine
keywords: Aluminum; DFO and DFP; FT-Raman; ICP-OES; Liver