title: Surface proteomic analysis of differentiated versus stem-like osteosarcoma human cells.
abstract: Cancer stem cell characterization represents a breakthrough in cancer research. Despite evidence showing the existence and the role of cancer stem cells in osteosarcoma (OS) onset and progression, little is known about their specific surface phenotype. To address this issue, we carried out a cytometric analysis with an antibody-array comprising 245 membrane proteins comparing the stem and differentiated OS cells. As experimental model, we chose the stem-like cell line 3aminobenzamide-OS and its parental, differentiated, cell line MG63. We identified 50 differentially expressed, 23 homogeneously expressed, and 172 not expressed proteins in the two cell line models, thus defining a surface protein signature specific for each of them. Furthermore, we selected ERK1/2 (p44/42 mitogen-activated protein kinases) as a potential pathway correlated with processes that characterize tumorigenic potential and stemness of 3aminobenzamide-OS cells.
authors.affiliation: CEINGE-Biotecnologie Avanzate s.c.a.r.l, Napoli, Italy.
authors.forename: Marica
authors.initials: M
authors.lastname: Gemei
authors.forename: Claudia
authors.initials: C
authors.lastname: Corbo
authors.forename: Francesca
authors.initials: F
authors.lastname: D'Alessio
authors.forename: Rosa
authors.initials: R
authors.lastname: Di Noto
authors.forename: Renza
authors.initials: R
authors.lastname: Vento
authors.forename: Luigi
authors.initials: L
authors.lastname: Del Vecchio
mesh_terms: D002454:Cell Differentiation / Q000502:physiology*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000737:chemistry / Q000378:metabolism*; D012516:Osteosarcoma / Q000737:chemistry / Q000378:metabolism*; D060066:Protein Interaction Maps / Q000502:physiology; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods
chemical_list: D008565:Membrane Proteins; D020543:Proteome
keywords: Cancer stem cell; Cell biology; Osteosarcoma; Surface proteome