title: Quantitative fluorescence spectral analysis of protein denaturation.
abstract: This chapter describes a procedure of global analysis of the steady-state spectra measured with different concentrations of the denaturant to quantitatively study protein denaturation. With the help of physicochemical models, relevant spectral parameters that characterize the folding intermediate and thermodynamic parameters that describe a three-state model N-I-U can be estimated.
authors.affiliation: Faculty of Sciences, Department of Physics and Astronomy, VU University Amsterdam, Amsterdam, The Netherlands.
authors.forename: Ivo H M
authors.initials: IH
authors.lastname: van Stokkum
authors.forename: Sergey P
authors.initials: SP
authors.lastname: Laptenok
mesh_terms: D016948:Azotobacter vinelandii / Q000737:chemistry*; D005453:Fluorescence; D007700:Kinetics; D008958:Models, Molecular; D011489:Protein Denaturation; D017510:Protein Folding; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics