title: Colorimetric detection of human papilloma virus by double isothermal amplification.
abstract: We developed a polymerase reaction free, low-cost and sensitive assay for the colorimetric detection of Human Papilloma Virus (HPV), based on the use of a smart design exploiting magnetic microbeads, chimeric RNA/DNAzyme oligonucleotides, and double signal amplification. This method allows obtaining a fast response with a detection limit of 10 pM, avoiding the amplification of the target via traditional PCR.
authors.affiliation: Istituto Italiano di Tecnologia (IIT), Center for Bio-Molecular Nanotechnologies@UniLe, Via Barsanti - 73010 Arnesano, Lecce, Italy. pierpaolo.pompa@iit.it.
authors.forename: Stefano
authors.initials: S
authors.lastname: Persano
authors.forename: Paola
authors.initials: P
authors.lastname: Valentini
authors.forename: Joong Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Pompa
mesh_terms: D003124:Colorimetry; D021881:DNA, Catalytic / Q000737:chemistry / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D010084:Oxidation-Reduction; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016914:Ribonuclease H / Q000378:metabolism; D014773:Virology / Q000379:methods*
chemical_list: D021881:DNA, Catalytic; D004279:DNA, Viral; D016914:Ribonuclease H