title: Electroacupuncture Stimulation at CV12 Inhibits Gastric Motility via TRPV1 Receptor.
abstract: Gastric dysmotility is one of the major pathophysiological factors in functional gastrointestinal disorders. Acupuncture, as one of the alternative approaches, is efficacious in the treatment of gastrointestinal motility disorders; however, the mechanism underlying its action is unclear. In the present study, we used both capsazepine, a TRPV1 antagonist, and TRPV1 knockout mice. Animals were divided into wild-type group (WT), capsazepine injection group (CZP, 0.5 mg/kg, i.p.), and TRPV1 knockout mice group (TRPV1(-/-)). Each of these three groups was divided into three subgroups, which were subjected to EA stimulation at acupoint Zhongwan (CV12) at a different intensity (1, 2, or 4 mA). We demonstrated that electroacupuncture at Zhongwan (CV12) markedly inhibited gastric motility at 2 and 4 mA in an intensity-dependent manner in wild-type mice. The inhibitory effect was also observed in capsazepine-injected and TRPV1(-/-) mice but was no longer intensity dependent, indicating that TRPV1 is partially involved in the electroacupuncture-mediated modulation of gastric motility.
authors.affiliation: Key Laboratory of Integrated Acupuncture and Drugs Constructed by the Ministry of Education and Jiangsu Province, Nanjing University of Chinese Medicine, Nanjing 210029, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yu
authors.forename: Xin
authors.initials: X
authors.lastname: Cao
authors.forename: Youbing
authors.initials: Y
authors.lastname: Xia
authors.forename: Binbin
authors.initials: B
authors.lastname: Ren
authors.forename: Hong
authors.initials: H
authors.lastname: Feng
authors.forename: Yali
authors.initials: Y
authors.lastname: Wang
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Jiang
authors.forename: Bin
authors.initials: B
authors.lastname: Xu