title: A novel highly reactive Fab antibody for breast cancer tissue diagnostics and staging also discriminates a subset of good prognostic triple-negative breast cancers.
abstract: The discovery of novel markers for breast cancer (BC) has been recently relied on antibody combinatorial libraries and selection through phage display. We constructed a recombinant Fab library, and after selections against BC tissues, the FabC4 clone was thoroughly investigated by immunohistochemistry in 232 patients with long-term follow-up. The FabC4 ligand was determined by mass spectrometry. The FabC4 expression was associated with younger age, lack of progesterone receptor, higher histological grades and non-luminal subtypes, and it also identified a subset of good prognostic triple-negative BCs, possibly targeting a conformational epitope of Cytokeratin-10 (CK10). This new CK10-epitope specific antibody may open new possibilities in diagnostic and therapeutic strategies.
authors.affiliation: Federal University of Uberlandia, Institute of Genetics and Biochemistry, Nanobiotechnology Laboratory, Campus Umuarama, Bloco 2E, Sala 248, 38400-902 Uberlandia, MG, Brazil.
authors.forename: Thaise G
authors.initials: TG
authors.lastname: Araújo
authors.affiliation: Division of Breast and Gynecological Tumors, Department of Clinical Oncology, Barretos Cancer Hospital, Barretos, SP, Brazil.
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Paiva
authors.affiliation: AC Camargo Cancer Hospital, São Paulo, SP, Brazil.
authors.forename: Rafael M
authors.initials: RM
authors.lastname: Rocha
authors.affiliation: Federal University of Uberlandia, Institute of Genetics and Biochemistry, Nanobiotechnology Laboratory, Campus Umuarama, Bloco 2E, Sala 248, 38400-902 Uberlandia, MG, Brazil.
authors.forename: Yara C P
authors.initials: YC
authors.lastname: Maia
authors.affiliation: Federal University of Uberlandia, Institute of Genetics and Biochemistry, Nanobiotechnology Laboratory, Campus Umuarama, Bloco 2E, Sala 248, 38400-902 Uberlandia, MG, Brazil.
authors.forename: Angela A S
authors.initials: AA
authors.lastname: Sena
authors.affiliation: Federal University of Uberlandia, Institute of Genetics and Biochemistry, Nanobiotechnology Laboratory, Campus Umuarama, Bloco 2E, Sala 248, 38400-902 Uberlandia, MG, Brazil.
authors.forename: Carlos
authors.initials: C
authors.lastname: Ueira-Vieira
authors.affiliation: Federal University of Uberlandia, Institute of Genetics and Biochemistry, Nanobiotechnology Laboratory, Campus Umuarama, Bloco 2E, Sala 248, 38400-902 Uberlandia, MG, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Carneiro
authors.affiliation: Federal University of Uberlandia, Institute of Genetics and Biochemistry, Nanobiotechnology Laboratory, Campus Umuarama, Bloco 2E, Sala 248, 38400-902 Uberlandia, MG, Brazil.
authors.forename: Juliana F
authors.initials: JF
authors.lastname: Almeida
authors.affiliation: Federal University of Uberlandia, Biomedical Sciences Institute, Uberlandia, MG, Brazil.
authors.forename: Paulo R
authors.initials: PR
authors.lastname: de Faria
authors.affiliation: Obstetric Division, Internal Medicine, University Hospital, Federal University of Uberlandia, Uberlandia, MG, Brazil.
authors.forename: Donizeti W
authors.initials: DW
authors.lastname: Santos
authors.affiliation: Obstetric Division, Internal Medicine, University Hospital, Federal University of Uberlandia, Uberlandia, MG, Brazil.
authors.forename: Luanda
authors.initials: L
authors.lastname: Calábria
authors.affiliation: Pathology Division, Internal Medicine, University Hospital, Federal University of Uberlandia, Uberlandia, MG, Brazil.
authors.forename: Tânia M
authors.initials: TM
authors.lastname: Alcântara
authors.affiliation: AC Camargo Cancer Hospital, São Paulo, SP, Brazil.
authors.forename: Fernando A
authors.initials: FA
authors.lastname: Soares
authors.affiliation: Federal University of Uberlandia, Institute of Genetics and Biochemistry, Nanobiotechnology Laboratory, Campus Umuarama, Bloco 2E, Sala 248, 38400-902 Uberlandia, MG, Brazil; University of California Davis, Dept. of Medical Microbiology and Immunology, Davis, CA, USA. Electronic address: lrgoulart@ufu.br.
authors.forename: Luiz R
authors.initials: LR
authors.lastname: Goulart
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D001943:Breast Neoplasms / Q000175:diagnosis*; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000235:genetics; D013058:Mass Spectrometry; D046228:Microarray Analysis; D008875:Middle Aged; D009367:Neoplasm Staging; D019151:Peptide Library; D011379:Prognosis; D064726:Triple Negative Breast Neoplasms / Q000175:diagnosis*
chemical_list: D007140:Immunoglobulin Fab Fragments; D019151:Peptide Library
keywords: Breast cancer; Combinatorial library; Phage display; Recombinant antibody