title: Detection and quantification of hepatopancreatic parvovirus in penaeid shrimp by real-time PCR assay.
abstract: As one of the major pathogens, hepatopancreatic parvovirus (HPV) can cause severe diseases in penaeid shrimp. We developed a TaqMan-based real-time PCR assay for the HPV detection in China. A pair of primers (HPVF and HPVR) and a TaqMan probe were designed according to the HPV genomic sequence of Chinese isolate (GenBank: GU371276). Our data showed that the primers and TaqMan probe were specific for HPV, and they exhibited no cross-reaction with infectious hypodermal and hematopoietic necrosis virus (IHHNV), white spot syndrome virus (WSSV) and specific pathogen free (SPF) shrimp DNA. The assay had a detection limit of four plasmid HPV DNA copies per reaction. Furthermore, HPV was detected in 16 of 21 Fenneropenaeus Chinensis, 3 of 52 Litopenaeus vannamei and 2 of 2 Marsupenaeus japonicus penaeid shrimp samples. In addition, HPV was also detected in crabs. Therefore, this assay could be successfully used as a sensitive and rapid molecular-based diagnostic method to screen HPV-free animals and survey the prevalence of HPV in cultured populations of penaeid shrimp in China.
authors.affiliation: Maricultural Organism Diseases Control and Molecular Pathology Laboratory, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 266071, PR China; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, PR China.
authors.forename: Tianqi
authors.initials: T
authors.lastname: Liu
authors.forename: Bing
authors.initials: B
authors.lastname: Yang
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Song
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Wang
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Huang
mesh_terms: D000818:Animals; D008969:Molecular Sequence Data; D010321:Parvoviridae / Q000235:genetics* / Q000302:isolation & purification; D033561:Penaeidae / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results
keywords: Detection; Hepatopancreatic parvovirus; Real-time PCR; TaqMan