title: Crystallization and preliminary X-ray study of a (2R,3R)-2,3-butanediol dehydrogenase from Bacillus coagulans 2-6.
abstract: (2R,3R)-2,3-Butanediol dehydrogenase (R,R-BDH) from Bacillus coagulans 2-6 is a zinc-dependent medium-chain alcohol dehydrogenase. Recombinant R,R-BDH with a His6 tag at the C-terminus was expressed in Escherichia coli BL21 (DE3) cells and purified by Ni2+-chelating affinity and size-exclusion chromatography. Crystals were grown by the hanging-drop vapour-diffusion method at 289 K. The crystallization condition consisted of 8%(v/v) Tacsimate pH 4.6, 18%(w/v) polyethylene glycol 3350. The crystal diffracted to 2.8 Å resolution in the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a=88.35, b=128.73, c=131.03 Å.
authors.affiliation: Tianjin University of Science and Technology, Tianjin 300222, People's Republic of China.
authors.forename: Xiangzhi
authors.initials: X
authors.lastname: Miao
authors.forename: Xianhui
authors.initials: X
authors.lastname: Huang
authors.forename: Guofang
authors.initials: G
authors.lastname: Zhang
authors.forename: Xiufang
authors.initials: X
authors.lastname: Zhao
authors.forename: Xianming
authors.initials: X
authors.lastname: Zhu
authors.forename: Hui
authors.initials: H
authors.lastname: Dong
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D001407:Bacillus / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel
chemical_list: D000429:Alcohol Oxidoreductases; C019801:butanediol dehydrogenase
keywords: (2R,3R)-2,3-butanediol dehydrogenase; Bacillus coagulans