title: Nanoplatforms for highly sensitive fluorescence detection of cancer-related proteases.
abstract: Numerous proteases are known to be necessary for cancer development and progression including matrix metalloproteinases (MMPs), tissue serine proteases, and cathepsins. The goal of this research is to develop an Fe/Fe3O4 nanoparticle-based system for clinical diagnostics, which has the potential to measure the activity of cancer-associated proteases in biospecimens. Nanoparticle-based "light switches" for measuring protease activity consist of fluorescent cyanine dyes and porphyrins that are attached to Fe/Fe3O4 nanoparticles via consensus sequences. These consensus sequences can be cleaved in the presence of the correct protease, thus releasing a fluorescent dye from the Fe/Fe3O4 nanoparticle, resulting in highly sensitive (down to 1 × 10(-16) mol l(-1) for 12 proteases), selective, and fast nanoplatforms (required time: 60 min).
authors.affiliation: Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USA. hongwang@ksu.edu dinu@ksu.edu sbossman@ksu.edu.
authors.forename: Hongwang
authors.initials: H
authors.lastname: Wang
authors.forename: Dinusha N
authors.initials: DN
authors.lastname: Udukala
authors.forename: Thilani N
authors.initials: TN
authors.lastname: Samarakoon
authors.forename: Matthew T
authors.initials: MT
authors.lastname: Basel
authors.forename: Mausam
authors.initials: M
authors.lastname: Kalita
authors.forename: Gayani
authors.initials: G
authors.lastname: Abayaweera
authors.forename: Harshi
authors.initials: H
authors.lastname: Manawadu
authors.forename: Aruni
authors.initials: A
authors.lastname: Malalasekera
authors.forename: Colette
authors.initials: C
authors.lastname: Robinson
authors.forename: David
authors.initials: D
authors.lastname: Villanueva
authors.forename: Pamela
authors.initials: P
authors.lastname: Maynez
authors.forename: Leonie
authors.initials: L
authors.lastname: Bossmann
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Riedy
authors.forename: Jenny
authors.initials: J
authors.lastname: Barriga
authors.forename: Ni
authors.initials: N
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Higgins
authors.forename: Gaohong
authors.initials: G
authors.lastname: Zhu
authors.forename: Deryl L
authors.initials: DL
authors.lastname: Troyer
authors.forename: Stefan H
authors.initials: SH
authors.lastname: Bossmann
mesh_terms: D002138:Calibration; D002232:Carbocyanines / Q000737:chemistry; D016384:Consensus Sequence; D057075:Enzyme Assays / Q000379:methods*; D031541:Fluorescence Resonance Energy Transfer; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D053509:Matrix Metalloproteinase 13 / Q000737:chemistry / Q000378:metabolism; D036103:Nanotechnology / Q000379:methods*; D009369:Neoplasms / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism*; D011166:Porphyrins / Q000737:chemistry; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013499:Surface Properties
chemical_list: D002232:Carbocyanines; D058185:Magnetite Nanoparticles; D011166:Porphyrins; C085321:cyanine dye 5; C018395:tetracarboxyphenylporphine; D010447:Peptide Hydrolases; D053509:Matrix Metalloproteinase 13