title: Radioactive Chernobyl environment has produced high-oil flax seeds that show proteome alterations related to carbon metabolism during seed development.
abstract: Starting in 2007, we have grown soybean (Glycine max [L.] Merr. variety Soniachna) and flax (Linum usitatissimum, L. variety Kyivskyi) in the radio-contaminated Chernobyl area and analyzed the seed proteomes. In the second-generation flax seeds, we detected a 12% increase in oil content. To characterize the bases for this increase, seed development has been studied. Flax seeds were harvested in biological triplicate at 2, 4, and 6 weeks after flowering and at maturity from plants grown in nonradioactive and radio-contaminated plots in the Chernobyl area for two generations. Quantitative proteomic analyses based on 2-D gel electrophoresis (2-DE) allowed us to establish developmental profiles for 199 2-DE spots in both plots, out of which 79 were reliably identified by tandem mass spectrometry. The data suggest a statistically significant increased abundance of proteins associated with pyruvate biosynthesis via cytoplasmic glycolysis, L-malate decarboxylation, isocitrate dehydrogenation, and ethanol oxidation to acetaldehyde in early stages of seed development. This was followed by statistically significant increased abundance of ketoacyl-[acylcarrier protein] synthase I related to condensation of malonyl-ACP with elongating fatty acid chains. On the basis of these and previous data, we propose a preliminary model for plant adaptation to growth in a radio-contaminated environment. One aspect of the model suggests that changes in carbon assimilation and fatty acid biosynthesis are an integral part of plant adaptation.
authors.affiliation: Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences , Akademicka 2, P.O. Box 39A, Nitra 95007, Slovakia.
authors.forename: Katarína
authors.initials: K
authors.lastname: Klubicová
authors.forename: Maksym
authors.initials: M
authors.lastname: Danchenko
authors.forename: Ludovit
authors.initials: L
authors.lastname: Skultety
authors.forename: Valentyna V
authors.initials: VV
authors.lastname: Berezhna
authors.forename: Namik M
authors.initials: NM
authors.lastname: Rashydov
authors.forename: Martin
authors.initials: M
authors.lastname: Hajduch
mesh_terms: D000220:Adaptation, Biological / Q000235:genetics / Q000528:radiation effects*; D002244:Carbon / Q000378:metabolism; D002588:Cesium Radioisotopes / Q000032:analysis; D047852:Chernobyl Nuclear Accident; D015180:Electrophoresis, Gel, Two-Dimensional; D019597:Flax / Q000737:chemistry / Q000235:genetics* / Q000528:radiation effects*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000528:radiation effects*; D006019:Glycolysis; D008043:Linseed Oil / Q000032:analysis; D008954:Models, Biological; D020543:Proteome / Q000235:genetics / Q000528:radiation effects*; D019289:Pyruvic Acid / Q000378:metabolism; D012639:Seeds / Q000737:chemistry / Q000254:growth & development / Q000528:radiation effects; D012987:Soil / Q000737:chemistry; D013326:Strontium Radioisotopes / Q000032:analysis; D053719:Tandem Mass Spectrometry; D014455:Ukraine
chemical_list: D002588:Cesium Radioisotopes; D020543:Proteome; D012987:Soil; D013326:Strontium Radioisotopes; D002244:Carbon; D008043:Linseed Oil; D019289:Pyruvic Acid