title: The correlation of biochemical and morphologic parameters in the assessment of sperm maturity.
abstract: OBJECTIVE
  To examine the relationship between biochemical markers and morphologic sperm characteristics, including head, neck, and tail changes.
  
  
  METHODS
  The study evaluated 154 patients who went to the Andrology Laboratory of the Clinic of Urology, Clinical Center of Serbia. Patients were divided into 4 groups: normozoospermic, oligozoospermic, severe oligozoospermic, and asthenozoospermic, according to the sperm concentration and motility.
  
  
  RESULTS
  The differences in creatine kinase (CK) and CK-M levels between normozoospermic and the 2 groups of oligozoospermic patients were significantly different (P <.01). The CK and CK-M levels correlated negatively with sperm concentration and sperm motility, but correlated positively with the pathologic sperm form. Patients with CK values >0.093 have a total number of pathologic forms higher than 0.40 (87.5% sensitivity, 77.3% specificity, the area under the curve was 0.832, P <.001). Patients with CK values <0.09 U/L have normal spermatogenesis and pathologic disorder of the head <15%, neck <12%, and tail <10%.
  
  
  CONCLUSIONS
  The relation between sperm morphology and biochemical markers included in the maturation process is established during the sperm genesis process. If the results of these markers are used together with the morphology of the spermatozoa in the interpretation of infertility, it would lead us to better insight of the fertility potential of the each patient.
authors.affiliation: Clinic of Urology, Clinical Center of Serbia, Belgrade, Serbia; School of Medicine University of Belgrade, Belgrade, Serbia.
authors.forename: Otas
authors.initials: O
authors.lastname: Durutovic
authors.forename: Natasa
authors.initials: N
authors.lastname: Lalic
authors.forename: Dragica
authors.initials: D
authors.lastname: Milenkovic-Petronic
authors.forename: Nebojsa
authors.initials: N
authors.lastname: Bojanic
authors.forename: Dejan
authors.initials: D
authors.lastname: Djordjevic
authors.forename: Bogomir
authors.initials: B
authors.lastname: Milojevic
authors.forename: Nebojsa
authors.initials: N
authors.lastname: Ladjevic
authors.forename: Ana
authors.initials: A
authors.lastname: Mimic
authors.forename: Lidija
authors.initials: L
authors.lastname: Tulic
authors.forename: Zoran
authors.initials: Z
authors.lastname: Dzamic
authors.forename: Sava
authors.initials: S
authors.lastname: Micic
mesh_terms: D003402:Creatine Kinase / Q000097:blood*; D052283:Creatine Kinase, MM Form / Q000097:blood*; D006801:Humans; D007248:Infertility, Male / Q000097:blood*; D008297:Male; D012372:ROC Curve; D012680:Sensitivity and Specificity; D013077:Sperm Head / Q000473:pathology; D013079:Sperm Maturation / Q000502:physiology*; D013082:Sperm Tail / Q000473:pathology; D013094:Spermatozoa / Q000473:pathology* / Q000502:physiology
chemical_list: D003402:Creatine Kinase; D052283:Creatine Kinase, MM Form