title: Plastidic protein Cdf1 is essential in Arabidopsis embryogenesis.
abstract: Arabidopsis cell growth defect factor-1 (Cdf1 in yeast, At5g23040) was originally isolated as a cell growth suppressor of yeast from genetic screening. To investigate the in vivo role of Cdf1 in plants, a T-DNA insertion line was analyzed. A homozygous T-DNA insertion mutant (cdf1/cdf1) was embryo lethal and showed arrested embryogenesis at the globular stage. The Cdf1 protein, when fused with green fluorescent protein, was localized to the plastid in stomatal guard cells and mesophyll cells. A promoter-β-glucuronidase assay found expression of Cdf1 in the early heart stage of embryogenesis, suggesting that Cdf1 was essential for Arabidopsis embryogenesis during the transition of the embryo from the globular to heart stage.
authors.affiliation: Graduate School of Science and Engineering, Saitama University, 255 Shimo-okubo, Sakura-ku, Saitama, 338-8570, Japan, mkawai@mail.saitama-u.ac.jp.
authors.forename: Maki
authors.initials: M
authors.lastname: Kawai-Yamada
authors.forename: Minoru
authors.initials: M
authors.lastname: Nagano
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kakimoto
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Uchimiya
mesh_terms: D017360:Arabidopsis / Q000378:metabolism / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D005816:Genetic Complementation Test; D006579:Heterozygote; D009154:Mutation; D030821:Plants, Genetically Modified; D018087:Plastids / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D012639:Seeds / Q000254:growth & development*
chemical_list: D029681:Arabidopsis Proteins; C502170:CDF1 protein, Arabidopsis; D002352:Carrier Proteins; D012097:Repressor Proteins; C000592515:cell growth defect factor-1, Arabidopsis