title: Hydrogen-oxidizing hydrogenases 1 and 2 of Escherichia coli regulate the onset of hydrogen evolution and ATPase activity, respectively, during glucose fermentation at alkaline pH.
abstract: Simultaneous measurement of redox potential (Eh ) and determination of H2 evolution kinetics using a pair of titanium silicate and platinum redox electrodes in fermenting cultures of Escherichia coli wild type and different mutants lacking hydrogenases 1 (Hyd-1) or 2 (Hyd-2) revealed that Hyd-1 controls the onset of H2 evolution at slightly alkaline pH (pH 7.5) and under oxidizing Eh . In addition, Hyd-2 influences the N,N'-dicyclohexylcarbodiimide-inhibited ATPase activity in fermenting cells and thus regulates the proton F0 F1 -ATPase at the alkaline pH but under reducing Eh .
authors.affiliation: Department of Microbiology, Plants and Microbes Biotechnology, Faculty of Biology, Yerevan State University, Yerevan, Armenia.
authors.forename: Anna
authors.initials: A
authors.lastname: Poladyan
authors.forename: Karen
authors.initials: K
authors.lastname: Trchounian
authors.forename: R Gary
authors.initials: RG
authors.lastname: Sawers
authors.forename: Armen
authors.initials: A
authors.lastname: Trchounian
mesh_terms: D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation / Q000235:genetics; D005947:Glucose / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006864:Hydrogenase / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D053858:Metabolic Networks and Pathways / Q000235:genetics; D010084:Oxidation-Reduction
chemical_list: D029968:Escherichia coli Proteins; D006859:Hydrogen; D006864:Hydrogenase; D005947:Glucose
keywords: Escherichia coli; F0F1-ATPase; hydrogenase 1; hydrogenase 2; pH; redox potential