title: Therapeutic vaccination against the rhesus lymphocryptovirus EBNA-1 homologue, rhEBNA-1, elicits T cell responses to novel epitopes in rhesus macaques.
abstract: Epstein-Barr virus (EBV) is a vaccine/immunotherapy target due to its association with several human malignancies. EBNA-1 is an EBV protein consistently expressed in all EBV-associated cancers. Herein, EBNA-1-specific T cell epitopes were evaluated after AdC-rhEBNA-1 immunizations in chronically lymphocryptovirus-infected rhesus macaques, an EBV infection model. Preexisting rhEBNA-1-specific responses were augmented in 4/12 animals, and new epitopes were recognized in 5/12 animals after vaccinations. This study demonstrated that EBNA-1-specific T cells can be expanded by vaccination.
authors.affiliation: Division of Pathology, Emory Vaccine Center, Yerkes National Primate Research Center, Atlanta, Georgia, USA.
authors.forename: Eduardo L V
authors.initials: EL
authors.lastname: Silveira
authors.forename: Mark H
authors.initials: MH
authors.lastname: Fogg
authors.forename: Rachel M
authors.initials: RM
authors.lastname: Leskowitz
authors.forename: Hildegund C
authors.initials: HC
authors.lastname: Ertl
authors.forename: Roger W
authors.initials: RW
authors.lastname: Wiseman
authors.forename: David H
authors.initials: DH
authors.lastname: O'Connor
authors.forename: Paul
authors.initials: P
authors.lastname: Lieberman
authors.forename: Fred
authors.initials: F
authors.lastname: Wang
authors.forename: Francois
authors.initials: F
authors.lastname: Villinger
mesh_terms: D000818:Animals; D018604:Epitope Mapping; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D019309:Epstein-Barr Virus Nuclear Antigens / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D005260:Female; D006566:Herpesviridae Infections / Q000188:drug therapy / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D018143:Lymphocryptovirus / Q000235:genetics / Q000276:immunology*; D008253:Macaca mulatta / Q000235:genetics / Q000276:immunology / Q000821:virology; D008297:Male; D018419:Primate Diseases / Q000188:drug therapy / Q000276:immunology* / Q000821:virology; D013601:T-Lymphocytes / Q000276:immunology* / Q000821:virology; D014611:Vaccination; D014765:Viral Vaccines / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000276:immunology
chemical_list: D018984:Epitopes, T-Lymphocyte; D019309:Epstein-Barr Virus Nuclear Antigens; D014765:Viral Vaccines; C417029:EBV-encoded nuclear antigen 1