title: The isolation and characterization of a telomerase immortalized goat trophoblast cell line.
abstract: Trophoblast cells play vital roles in the processes of embryonic implantation and placentation. Many toxicological compounds can induce the malfunction of trophoblast cells, resulting in implantation failure or early embryonic loss. The finite lifespan of primary trophoblast cells limits investigation of the long-term effects of some toxicological compounds on trophoblast cells in vitro. In this study, primary goat trophoblast cells were purified by density gradient centrifugation and specific immuno-affinity purification. Then, the purified cells were immortalized through transfection of a plasmid containing the human telomerase reverse transcriptase (hTERT) gene. hTERT-transfected goat trophoblast cells (hTERT-GTCs) could steadily express hTERT gene and exhibit higher telomerase activity, and persistently proliferate without any signs of senescence up to 50 passages. The immortalized goat trophoblast cells still possessed the basic and key properties of normal primary goat trophoblast cells to express the specific intracellular marker cytokeratin 7 (CK-7) and secrete chorionic gonadotrophin β-subunit (CG-β) and placental lactogen (PL). Further studies showed that the immortalized goat trophoblast cells expressed vimentin and non-classical MHC class I antigen and exhibited invasive phenotype, suggesting that the immortalized goat trophoblasts resembled human extravillous trophoblasts. In addition, this cell line did not show neoplastic transformation either in vivo or in vitro. We concluded the immortalized goat trophoblast cells by hTERT transfection retained the basic and key characteristics of primary trophoblast cells and may provide a useful model to study the effects of some toxicological compounds on trophoblast cells.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: F
authors.initials: F
authors.lastname: Dong
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.forename: X
authors.initials: X
authors.lastname: Zhao
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: Q
authors.initials: Q
authors.lastname: Du
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Song
authors.forename: D
authors.initials: D
authors.lastname: Tong
mesh_terms: D000003:Abattoirs; D000818:Animals; D017849:Animals, Inbred Strains; D015415:Biomarkers / Q000378:metabolism; D002460:Cell Line; D002461:Cell Line, Transformed; D049109:Cell Proliferation; D002478:Cells, Cultured; D002681:China; D003612:Dairying; D005260:Female; D006041:Goats; D006801:Humans; D010920:Placenta / Q000166:cytology / Q000378:metabolism; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D011257:Pregnancy Proteins; D011994:Recombinant Proteins; C509186:TERT protein, human; D019098:Telomerase
keywords: CG-β; CK-7; FBS; FSA; GTCs; Goat trophoblast cells; Immortalization; PBS; PDT; PL; RIA; Telomerase; chorionic gonadotrophin β-subunit; cytokeratin 7; fetal bovine serum; fibroblast specific antigen; goat trophoblast cells; hTERT; human telomerase reverse transcriptase; phosphate buffered saline; placental lactogen; population doubling time; radioimmunoassay