title: Soluble form of canine transferrin receptor inhibits canine parvovirus infection in vitro and in vivo.
abstract: Canine parvovirus (CPV) disease is an acute, highly infectious disease threatening the dog-raising industry. So far there are no effective therapeutic strategies to control this disease. Although the canine transferrin receptor (TfR) was identified as a receptor for CPV infection, whether extracellular domain of TfR (called soluble TfR (sTfR)) possesses anti-CPV activities remains elusive. Here, we used the recombinant sTfR prepared from HEK293T cells with codon-optimized gene structure to investigate its anti-CPV activity both in vitro and in vivo. Our results indicated that codon optimization could significantly improve sTfR expression in HEK293T cells. The prepared recombinant sTfR possessed a binding activity to both CPV and CPV VP2 capsid proteins and significantly inhibited CPV infection of cultured feline F81 cells and decreased the mortality of CPV-infected dogs, which indicates that the sTfR has the anti-CPV activity both in vitro and in vivo.
authors.affiliation: Laboratory of Molecular Virology and Immunology, College of Veterinary Medicine, Agricultural University of Hebei, Baodiing 071001, China ; Hebei Engineering and Technology Research Center of Veterinary Biological Products, North China Research Center of Animal Epidemic Pathogen Biology, China Agriculture Ministry, Baoding 071001, China.
authors.forename: Jiexia
authors.initials: J
authors.lastname: Wen
authors.forename: Sumin
authors.initials: S
authors.lastname: Pan
authors.forename: Shuang
authors.initials: S
authors.lastname: Liang
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Zhong
authors.forename: Ying
authors.initials: Y
authors.lastname: He
authors.forename: Hongyu
authors.initials: H
authors.lastname: Lin
authors.forename: Wenyan
authors.initials: W
authors.lastname: Li
authors.forename: Liyue
authors.initials: L
authors.lastname: Wang
authors.forename: Xiujin
authors.initials: X
authors.lastname: Li
authors.forename: Fei
authors.initials: F
authors.lastname: Zhong
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D004285:Dogs / Q000235:genetics / Q000821:virology; D057809:HEK293 Cells; D006801:Humans; D010322:Parvoviridae Infections / Q000378:metabolism / Q000662:veterinary / Q000821:virology*; D017993:Parvovirus, Canine / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D011485:Protein Binding / Q000235:genetics; D011990:Receptors, Transferrin / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; D011990:Receptors, Transferrin; D011994:Recombinant Proteins