title: Crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1.
abstract: The plant hormones cytokinins play a central role in regulating cell division and developmental events. Cytokinin oxidase regulates the levels of these plant hormones by catalyzing their irreversible oxidation, which contributes to the regulation of various morpho-physiological processes controlled by cytokinins. In this study, the crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1 are reported. Plate-like crystals of LuCKX1.1 were obtained using PEG 3350 as a precipitant and diffracted X-rays to 1.78 Å resolution. The protein crystals have the symmetry of space group C2 and are most likely to contain two molecules per asymmetric unit.
authors.affiliation: School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience (Division of Chemistry and Structural Biology) and Australian Infectious Diseases Research Centre, University of Queensland, Brisbane, Queensland 4072, Australia.
authors.forename: Li
authors.initials: L
authors.lastname: Wan
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Williams
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Zhang
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Ericsson
authors.forename: Markus
authors.initials: M
authors.lastname: Koeck
authors.forename: Peter N
authors.initials: PN
authors.lastname: Dodds
authors.forename: Jeffrey G
authors.initials: JG
authors.lastname: Ellis
authors.forename: Bostjan
authors.initials: B
authors.lastname: Kobe
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D019597:Flax / Q000201:enzymology*; D010088:Oxidoreductases / Q000737:chemistry*
chemical_list: D010088:Oxidoreductases; C019984:cytokinin oxidase
keywords: cytokinin; cytokinin dehydrogenase; cytokinin oxidase; flavin adenine dinucleotide; plant hormones