title: Microfluidic cell culture system with on-chip hypoxic conditioning.
abstract: We have demonstrated a portable microfluidic cell culture system with multi-gas (CO2 and O2) incubation which we can cultivate under hypoxia without bulky peripheral apparatus such as gas tanks, regulators, and flow controllers. The system contains a chip of 26 mm × 48 mm which is capable to diffuse CO2 and absorb O2 through a gas-permeable wall of nested media reservoir. The media was water-jacketed with aqueous solution containing 0.8 M sodium bicarbonate as CO2 supply and 1 M sodium ascorbate as oxygen scavenger. The partial CO2 pressure (pCO2) in media reservoir stabilized at least 10.2% ± 0.11% for at least 72 hours. The partial O2 pressure (pO2) in the media reservoir decreased to 4.2%. Portable on-chip hypoxic culture of SV40-T2 cells for 72 h was also demonstrated.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Takano
authors.forename: Masato
authors.initials: M
authors.lastname: Tanaka
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Futai
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry; D002245:Carbon Dioxide / Q000737:chemistry*; D018929:Cell Culture Techniques; D015687:Cell Hypoxia; D002460:Cell Line; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D046210:Microfluidic Analytical Techniques; D044085:Microfluidics / Q000379:methods*; D010100:Oxygen / Q000737:chemistry*; D010539:Permeability; D051381:Rats
chemical_list: D004129:Dimethylpolysiloxanes; D002245:Carbon Dioxide; C013830:baysilon; D001205:Ascorbic Acid; D010100:Oxygen