title: Isolation and characterization of halophilic Bacillus sp. BS3 able to produce pharmacologically important biosurfactants.
abstract: OBJECTIVE
  To characterize the pharmacological importance of biosurfactants isolated from halophilic Bacillus sp BS3.
  
  
  METHODS
  Halophilic Bacillus sp. BS3 was isolated from solar salt works, identified by 16S rRNA sequencing and was used for screening their biosurfactant production. Characters of the biosurfactant and their anticancer activity were analyzed and performed in mammary epithelial carcinoma cell at different concentrations.
  
  
  RESULTS
  The biosurfactant were characterized by TLC, FTIR and GC-MS analysis and identified as lipopeptide type. GC-MS analysis revealed that, the biosurfactant had various compounds including 13-Docosenamide, (Z); Mannosamine, 9- and N,N,N',N'-tetramethyl. Surprisingly the antiviral activity was found against shrimp white spot syndrome virus (WSSV) by suppressing the viral replication and significantly raised shrimp survival (P<0.01). Anticancer activity performed in the mammary epithelial carcinoma cell at different concentrations of biosurfactants, among the various concentrations of biosurfactants such as 0.000 25, 0.002 5, 0.025, 0.25 and 2.5 μg, the 0.25 μg concentration suppressed the cells significantly (P<0.05) to 24.8%.
  
  
  CONCLUSIONS
  Based on the findings, the present study concluded that, there is a possibility to develop eco-friendly antimicrobial and anticancer drugs from the extremophilic origin.
authors.affiliation: Centre for Marine Science and Technology, Manonmaniam Sundaranar University Rajakkamangalam, Kanyakumari District, Tamilnadu, India 629502.
authors.forename: M B S
authors.initials: MB
authors.lastname: Donio
authors.forename: S F A
authors.initials: SF
authors.lastname: Ronica
authors.forename: V Thanga
authors.initials: VT
authors.lastname: Viji
authors.forename: S
authors.initials: S
authors.lastname: Velmurugan
authors.forename: J Adlin
authors.initials: JA
authors.lastname: Jenifer
authors.forename: M
authors.initials: M
authors.lastname: Michaelbabu
authors.forename: T
authors.initials: T
authors.lastname: Citarasu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D001407:Bacillus / Q000737:chemistry / Q000254:growth & development / Q000302:isolation & purification*; D018407:Bacterial Physiological Phenomena; D002855:Chromatography, Thin Layer; D015195:Drug Design; D006801:Humans; D008826:Microbial Sensitivity Tests; D012336:RNA, Ribosomal, 16S; D054712:Salinity; D017550:Spectroscopy, Fourier Transform Infrared; D013501:Surface-Active Agents / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000970:Antineoplastic Agents; D000998:Antiviral Agents; D012336:RNA, Ribosomal, 16S; D013501:Surface-Active Agents
keywords: Anticancer; Antimicrobial; Biosurfactants; Halophilic Bacillus sp BS3; Lipopeptide