title: Increased SPHK1 expression is associated with poor prognosis in bladder cancer.
abstract: Upregulation of sphingosine kinase 1 (SPHK1) protein has been reported to be associated with a poor prognosis in a variety of malignant tumors. However, the role of SPHK1 in bladder cancer (BC) has not been thoroughly elucidated. The purpose of this study was to assess SPHK1 expression and to explore its contribution to BC. Real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was conducted to detect SPHK1 mRNA expression in 37 pairs of fresh-frozen BC tissues and corresponding noncancerous tissues. Results showed that SPHK1 mRNA expression level in BC tissues was significantly higher than that in corresponding noncancerous tissues. To investigate the association between SPHK1 protein expression and clinicopathological characteristics of BC, immunohistochemistry (IHC) was performed in 153 archived paraffin-embedded BC samples. Interestingly, high SPHK1 expression was significantly associated with histologic grade (P = 0.045) and tumor stage (P < 0.001) of patients with BC. The Kaplan-Meier survival curve showed that patients with high SPHK1 expression had significantly reduced overall 5-year survival rates (P < 0.001). Multivariate Cox regression analysis further suggested that the increased expression of SPHK1 was an independent poor prognostic factor for this disease. In conclusion, our data offer the convincing evidence for the first time that the increased expression of SPHK1 may be involved in the pathogenesis and progression of BC. SPHK1 might be a potential marker to predict the prognosis in BC.
authors.affiliation: Department of Urology, Southwest Hospital, The Third Military Medical University, No. 30, Gaotanyanzheng Street, Shapingba District, Chongqing, 40038, China, mengxd1977@gmail.com.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Meng
authors.forename: Zhan-Song
authors.initials: ZS
authors.lastname: Zhou
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Qiu
authors.forename: Wen-Hao
authors.initials: WH
authors.lastname: Shen
authors.forename: Qu
authors.initials: Q
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Xiao
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000032:analysis / Q000096:biosynthesis*; D011379:Prognosis; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001749:Urinary Bladder Neoplasms / Q000201:enzymology* / Q000401:mortality* / Q000473:pathology; D000099157:Sphingosine Kinase
chemical_list: D014408:Biomarkers, Tumor; D017853:Phosphotransferases (Alcohol Group Acceptor); D000099157:Sphingosine Kinase