title: Quantitative determination of total and individual flavonoids in stems and leaves of Buddleja davidii and Buddleja albiflora.
abstract: BACKGROUND
  Buddleja davidii and B. albiflora are two different original plants of the famous crude medicine "Diaoyangchen".
  
  
  METHODS
  An ultraviolet-visible spectrophotometric method and a HPLC method were used for the determination of total and individual flavonoids (luteolin and apigenin) contents from their stems and leaves for the first time.
  
  
  RESULTS
  From the comparative evaluation, remarkable differences in flavonoids contents were observed between different origins and different parts of the samples. And content of specific flavonoid did not correspond to the total flavonoids contents in Buddleja davidii and Buddleja albiflora.
  
  
  CONCLUSIONS
  With a better accuracy and precision, the methods had been proved simple, rapid, and reliable for quantitative determination of the total flavonoids and luteolin and apigenin in the two phytomedicines. Furthermore, our present study will pave the way of guidelines for the differentiation and standardization and exploitation of individual parts of this herb material.
authors.affiliation: Department of Pharmacy, South-central University for Nationalities, Wuhan, Hubei Province, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Ying
authors.forename: Dingrong
authors.initials: D
authors.lastname: Wan
keywords: Apigenin; Buddleja albiflora Hemsl.; Buddleja daviddi Franch.; luteolin; total flavonoids