title: Crystallization and preliminary X-ray diffraction studies of Tyr167His mutant α-cyclodextrin glucanotransferase from Bacillus macerans.
abstract: Improving the specificity of α-cyclodextrin glucanotransferase is a significant issue in the field of α-cyclodextrin production. In this study, a constructed Y167H mutant α-cyclodextrin glucanotransferase with enhanced α-cyclodextrin specificity was successfully expressed and purified. Single crystals were grown using PEG 4000 as a precipitating agent by the hanging-drop vapour-diffusion method at 293 K. The crystals exhibited two kinds of morphology in different crystallization conditions. The crystals diffracted to at least 2.2 Å resolution (space group P2₁2₁2₁), with unit-cell parameters a=65.69, b=78.70, c=137.00 Å. Assuming the asymmetric cell to be occupied by a monomer of 75 kDa, the unit cell contains 43.77% solvent with a crystal volume per protein mass, VM, of 2.19 Å3 Da(-1).
authors.affiliation: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University (BUAA), Beijing 100191, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yue
authors.forename: Shengquan
authors.initials: S
authors.lastname: Liu
authors.forename: Hongbin
authors.initials: H
authors.lastname: Li
authors.forename: Binghong
authors.initials: B
authors.lastname: Song
authors.forename: Ting
authors.initials: T
authors.lastname: Xie
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Yapeng
authors.initials: Y
authors.lastname: Chao
authors.forename: Shijun
authors.initials: S
authors.lastname: Qian
mesh_terms: D019943:Amino Acid Substitution; D001407:Bacillus / Q000201:enzymology*; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D005964:Glucosyltransferases / Q000737:chemistry*; D050505:Mutant Proteins / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D050505:Mutant Proteins; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase
keywords: α-cyclodextrin glucanotransferase