title: Fast-pulsing NMR techniques for the detection of weak interactions: successful natural abundance probe of hydrogen bonds in peptides.
abstract: Structural investigations of peptides using NMR spectroscopy rarely include the detection of N-H···O=C and N-H···N hydrogen bonds, because the relevant heteronuclei have a low natural abundance while the small trans hydrogen bond scalar couplings reduce the sensitivity. Fast repetition NMR techniques combined with state of the art spectrometer specifications allowed the enhancement of the sensitivity for detection of hydrogen bonds at natural isotopic abundance.
authors.affiliation: Université Paris Sud, ICMMO UMR 8182, ESOM, 15 Rue Georges Clemenceau, bât 420, 91405 Orsay, France.
authors.forename: Amandine
authors.initials: A
authors.lastname: Altmayer-Henzien
authors.forename: Valérie
authors.initials: V
authors.lastname: Declerck
authors.forename: David J
authors.initials: DJ
authors.lastname: Aitken
authors.forename: Ewen
authors.initials: E
authors.lastname: Lescop
authors.forename: Denis
authors.initials: D
authors.lastname: Merlet
authors.forename: Jonathan
authors.initials: J
authors.lastname: Farjon
mesh_terms: D006860:Hydrogen Bonding; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000737:chemistry*; D017433:Protein Structure, Secondary
chemical_list: D010455:Peptides