title: Development of automated 3-dimensional tissue fabrication system Tissue Factory - Automated cell isolation from tissue for regenerative medicine.
abstract: We have developed a new automated cell isolation system as one of the modules of automated cell sheet production system named Tissue-Factory (T-Factory). This system enables isolation of the target cells from tissue. Using this new system, we successfully isolated skeletal myoblast from skeletal muscle tissue. The cell isolation system makes us stably prepare cell suspension from each tissue automatically and safely. Isolation of skeletal myoblasts will contribute to labor-saving cell cultivation and operational stability, and lead further process in tissue engineering and regenerative medicine.
authors.forename: H
authors.initials: H
authors.lastname: Kubo
authors.forename: T
authors.initials: T
authors.lastname: Shioyama
authors.forename: M
authors.initials: M
authors.lastname: Oura
authors.forename: A
authors.initials: A
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
authors.forename: H
authors.initials: H
authors.lastname: Makino
authors.forename: S
authors.initials: S
authors.lastname: Takeda
authors.forename: M
authors.initials: M
authors.lastname: Kino-oka
authors.forename: T
authors.initials: T
authors.lastname: Shimizu
authors.forename: T
authors.initials: T
authors.lastname: Okano
authors.forename: S
authors.initials: S
authors.lastname: Yamamori
mesh_terms: D000818:Animals; D001331:Automation; D001706:Biopsy; D002469:Cell Separation; D002478:Cells, Cultured; D004867:Equipment Design; D008856:Microscopy, Fluorescence; D018482:Muscle, Skeletal / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D044968:Regenerative Medicine / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods