title: Chemical inhibition of acetyl coenzyme A carboxylase as a strategy to increase polyhydroxybutyrate yields in transgenic sugarcane.
abstract: Polyhydroxybutyrate (PHB) is a naturally occurring bacterial polymer that can be used as a biodegradable replacement for some petrochemical-derived plastics. Polyhydroxybutyrate is produced commercially by fermentation, but to reduce production costs, efforts are underway to produce it in engineered plants, including sugarcane. However, PHB levels in this high-biomass crop are not yet commercially viable. Chemical ripening with herbicides is a strategy used to enhance sucrose production in sugarcane and was investigated here as a tool to increase PHB production. Class A herbicides inhibit ACCase activity and thus reduce fatty acid biosynthesis, with which PHB production competes directly for substrate. Treatment of PHB-producing transgenic sugarcane plants with 100 μM of the class A herbicide fluazifop resulted in a fourfold increase in PHB content in the leaves, which peaked ten days post-treatment. The minimum effective concentration of herbicide required to maximize PHB production was 30 μM for fluazifop and 70 μM for butroxydim when applied to saturation. Application of a range of class A herbicides from the DIM and FOP groups consistently resulted in increased PHB yields, particularly in immature leaf tissue. Butroxydim or fluazifop treatment of mature transgenic sugarcane grown under glasshouse conditions increased the total leaf biomass yield of PHB by 50%-60%. Application of an ACCase inhibitor in the form of a class A herbicide to mature sugarcane plants prior to harvest is a promising strategy for improving overall PHB yield. Further testing is required on field-grown transgenic sugarcane to more precisely determine the effectiveness of this strategy.
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, the University of Queensland, Brisbane, Qld, Australia.
authors.forename: Lars A
authors.initials: LA
authors.lastname: Petrasovits
authors.forename: Richard B
authors.initials: RB
authors.lastname: McQualter
authors.forename: Leigh K
authors.initials: LK
authors.lastname: Gebbie
authors.forename: Deborah M
authors.initials: DM
authors.lastname: Blackman
authors.forename: Lars K
authors.initials: LK
authors.lastname: Nielsen
authors.forename: Stevens M
authors.initials: SM
authors.lastname: Brumbley
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D018533:Biomass; D018506:Gene Expression Regulation, Plant; D005818:Genetic Engineering; D006540:Herbicides / Q000494:pharmacology*; D006885:Hydroxybutyrates / Q000378:metabolism*; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D031786:Saccharum / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
chemical_list: D006540:Herbicides; D006885:Hydroxybutyrates; D000103:Acetyl-CoA Carboxylase
keywords: biopolymer; chemical ripener; herbicide; polyhydroxybutyrate; sugarcane