title: New generation of ensemble-decision aliquot ranking based on simplified microfluidic components for large-capacity trapping of circulating tumor cells.
abstract: Ensemble-decision aliquot ranking (eDAR) is a sensitive and high-throughput method to analyze circulating tumor cells (CTCs) from peripheral blood. Here, we report the next generation of eDAR, where we designed and optimized a new hydrodynamic switching scheme for the active sorting step in eDAR, which provided fast cell sorting with an improved reproducibility and stability. The microfluidic chip was also simplified by incorporating a functional area for subsequent purification using microslits fabricated by standard lithography method. Using the reported second generation of eDAR, we were able to analyze 1 mL of whole-blood samples in 12.5 min, with a 95% recovery and a zero false positive rate (n = 15).
authors.affiliation: Department of Chemistry, University of Washington , Seattle, Washington 98195, United States.
authors.forename: Mengxia
authors.initials: M
authors.lastname: Zhao
authors.forename: Wyatt C
authors.initials: WC
authors.lastname: Nelson
authors.forename: Bingchuan
authors.initials: B
authors.lastname: Wei
authors.forename: Perry G
authors.initials: PG
authors.lastname: Schiro
authors.forename: Bejan M
authors.initials: BM
authors.lastname: Hakimi
authors.forename: Eleanor S
authors.initials: ES
authors.lastname: Johnson
authors.forename: Robbyn K
authors.initials: RK
authors.lastname: Anand
authors.forename: Grace S
authors.initials: GS
authors.lastname: Gyurkey
authors.forename: Lisa M
authors.initials: LM
authors.lastname: White
authors.forename: Samuel H
authors.initials: SH
authors.lastname: Whiting
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Coveler
authors.forename: Daniel T
authors.initials: DT
authors.lastname: Chiu
mesh_terms: D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D057446:Hydrodynamics; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D009362:Neoplasm Metastasis; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D010190:Pancreatic Neoplasms / Q000473:pathology; D012825:Silicon / Q000737:chemistry
chemical_list: D012825:Silicon