title: Noninterferometric single-shot quantitative phase microscopy.
abstract: We present a noninterferometric single-shot quantitative phase microscopy technique with the use of the transport of intensity equation (TIE). The optical configuration is based on a Michelson-like architecture attached to a nonmodified inverted transmission bright field microscope. Two laterally separated images from different focal planes can be obtained simultaneously by a single camera exposure, enabling the TIE phase recovery to be performed at frame rates that are only camera limited. Precise measurement of a microlens array validates the principle and demonstrates the accuracy of the method. Investigations of chemical-induced apoptosis and the phagocytosis process of macrophages are then presented, suggesting that the method developed can provide promising applications in the dynamic study of cellular processes.
authors.forename: Chao
authors.initials: C
authors.lastname: Zuo
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.forename: Weijuan
authors.initials: W
authors.lastname: Qu
authors.forename: Anand
authors.initials: A
authors.lastname: Asundi
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D008264:Macrophages / Q000166:cytology; D051379:Mice; D008853:Microscopy / Q000379:methods*; D055620:Optical Phenomena; D010587:Phagocytosis