title: A novel single compartment in vitro model: Perflurocarbons for electrophysiological studies of the rat urinary bladder.
abstract: This study presents a novel single compartment model for in vitro electrophysiological studies of the rat urinary bladder. We tested the functionality and suitability of FC-770 (a Perflurocarbon) for in vitro recording of nerve activity arising from the bladder in a single compartment setup. We have also favorably tested stimulation of the bladder via the bladder nerves in FC-770. The organ viability was monitored by recording spontaneous contractions of the bladder for a certain time. We propose the use of FC-770 as a fluid for nerve recording/stimulation in vitro as well as for maintaining organ viability, over the commonly used two compartmental methods.
authors.forename: M S
authors.initials: MS
authors.lastname: Choudhary
authors.forename: E
authors.initials: E
authors.lastname: van Asselt
authors.forename: R
authors.initials: R
authors.lastname: van Mastrigt
authors.forename: F
authors.initials: F
authors.lastname: Clavica
mesh_terms: D000818:Animals; D004558:Electric Stimulation; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods*; D005466:Fluorocarbons / Q000737:chemistry*; D066298:In Vitro Techniques; D051381:Rats; D011923:Rats, Nude; D017208:Rats, Wistar; D012815:Signal Processing, Computer-Assisted; D001743:Urinary Bladder / Q000294:innervation* / Q000502:physiology*
chemical_list: D005466:Fluorocarbons