title: Purification and characterization of α-neoagarooligosaccharide hydrolase from Cellvibrio sp. OA-2007.
abstract: α-Neoagarooligosaccharide (α-NAOS) hydrolase was purified from Cellvibrio sp. OA-2007 by using chromatographic techniques after hydroxyapatite adsorption. The molecular masses of α-NAOS hydrolase estimated using SDS-PAGE and gel filtration chromatography were 40 and 93 kDa, respectively, and the optimal temperature and pH for the enzyme activity were 32ºC and 7.0-7.2. α-NAOS hydrolase lost 43% of its original activity when incubated at 35ºC for 30 min. The enzyme hydrolyzed neoagarobiose, neoagarotetraose, and neoagarohexaose to galactose, agarotriose, and agaropentaose, respectively, and produced 3,6-anhydro-L-galactose concomitantly; however, it did not degrade agarose.
authors.affiliation: School of Environmental Science and Engineering, Kochi University of Technology,782-8502, Kochi, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Ariga
authors.forename: Naoki
authors.initials: N
authors.lastname: Okamoto
authors.forename: Naomi
authors.initials: N
authors.lastname: Harimoto
authors.forename: Kiyohiko
authors.initials: K
authors.lastname: Nakasaki
mesh_terms: D020633:Cellvibrio / Q000201:enzymology*; D002850:Chromatography, Gel; D002853:Chromatography, Liquid; D004187:Disaccharides / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D005690:Galactose; D005697:Galactosides / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D009844:Oligosaccharides / Q000378:metabolism; D013696:Temperature
chemical_list: D004187:Disaccharides; D005697:Galactosides; D009844:Oligosaccharides; C081798:neoagarotetraose; C068544:neoagarobiose; D006026:Glycoside Hydrolases; C090400:alpha-neoagarooligosaccharide hydrolase; D005690:Galactose