title: Influence of fibre diameter and orientation of electrospun copolyetheresterurethanes on smooth muscle and endothelial cell behaviour.
abstract: Polymers exhibiting cell-selective effects represent an extensive research field with high relevance for biomedical applications e.g. in the cardiovascular field supporting re-endothelialization while suppressing smooth muscle cell overgrowth. Such an endothelial cell-selective effect could be recently demonstrated for a copolyetheresterurethane (PDC) containing biodegradable poly(p-dioxanone) and poly(ε-caprolactone) segments, which selectively enhanced the adhesion of human umbilical vein endothelial cells (HUVEC) while suppressing the attachment of smooth muscle cells (SMC). In this study we investigated the influence of the fibre orientation (random and aligned) and fibre diameter (2 μm and 500 nm) of electrospun PDC scaffolds on the adhesion, proliferation and apoptosis of HUVEC and SMC. Adhesion, viability and proliferation of HUVEC was diminished when the fibre diameter was reduced to a submicron scale, while the orientation of the microfibres did only slightly influence the cellular behaviour. In contrast, a submicron fibre diameter improved SMC viability. In conclusion, PDC scaffolds with micron-sized single fibres could be promising candidate materials for cell-selective stent coatings.
authors.affiliation: Berlin-Brandenburg Center for Regenerative Therapies, Berlin, Germany Department of Cardiology, Campus Virchow Klinikum, Charité, Berlin, Germany.
authors.forename: Constantin
authors.initials: C
authors.lastname: Rüder
authors.affiliation: Berlin-Brandenburg Center for Regenerative Therapies, Berlin, Germany Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, Teltow, Germany Institute of Biochemistry and Biology, University of Potsdam, Potsdam-Golm, Germany.
authors.forename: Tilman
authors.initials: T
authors.lastname: Sauter
authors.affiliation: Berlin-Brandenburg Center for Regenerative Therapies, Berlin, Germany Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
authors.forename: Karl
authors.initials: K
authors.lastname: Kratz
authors.affiliation: Berlin-Brandenburg Center for Regenerative Therapies, Berlin, Germany Department of Cardiology, Campus Virchow Klinikum, Charité, Berlin, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Haase
authors.affiliation: Berlin-Brandenburg Center for Regenerative Therapies, Berlin, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Peter
authors.affiliation: Berlin-Brandenburg Center for Regenerative Therapies, Berlin, Germany Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
authors.forename: Friedrich
authors.initials: F
authors.lastname: Jung
authors.affiliation: Berlin-Brandenburg Center for Regenerative Therapies, Berlin, Germany Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, Teltow, Germany Institute of Biochemistry and Biology, University of Potsdam, Potsdam-Golm, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Lendlein
authors.affiliation: Berlin-Brandenburg Center for Regenerative Therapies, Berlin, Germany Department of Cardiology, Campus Virchow Klinikum, Charité, Berlin, Germany.
authors.forename: Dietlind
authors.initials: D
authors.lastname: Zohlnhöfer
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D048708:Cell Growth Processes / Q000502:physiology; D002478:Cells, Cultured; D004146:Dioxanes / Q000737:chemistry; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D008422:Materials Testing / Q000379:methods; D008855:Microscopy, Electron, Scanning; D009130:Muscle, Smooth / Q000166:cytology* / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D004146:Dioxanes; D011091:Polyesters; D011108:Polymers; D011140:Polyurethanes; C016240:polycaprolactone; C079733:poly-4-dioxan-2-one
keywords: Endothelialization; cell selectivity; degradable polymer; drug eluting stent; electrospinning