title: Metabolomic investigation of methicillin-resistant Staphylococcus aureus.
abstract: Metabolomics is becoming increasingly important as it provides a comprehensive analytical platform to better understand the biological functioning of a cell or organism. In recent years, microbial metabolomics has received much attention in research areas from new drug discovery to metabolic engineering. An efficient and accurate method to measure the intracellular metabolites of a specific microbial species is a key prerequisite for metabolome analysis. In this study, we describe a workflow focusing on the extraction and quantification of intracellular metabolites of Staphylococcus aureus. A filter-based bacteria sampling system was utilized to separate the media and bacteria; fast quenching with nitrogen was applied to prevent any metabolite leakage; a glass beads beater was used for intracellular metabolite extraction; and the LC-QTOF was combined to quantify the intracellular amino acids of S. aureus. This protocol is demonstrated to be an efficient method for analyzing the intracellular metabolites of S. aureus.
authors.affiliation: Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA.
authors.forename: Ting
authors.initials: T
authors.lastname: Lei
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Chi
authors.initials: C
authors.lastname: Chen
authors.forename: Yinduo
authors.initials: Y
authors.lastname: Ji
mesh_terms: D000596:Amino Acids / Q000032:analysis / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification / Q000378:metabolism*; D013203:Staphylococcal Infections / Q000382:microbiology*
chemical_list: D000596:Amino Acids