title: Utilization of palm oil decanter cake as a novel substrate for biosurfactant production from a new and promising strain of Ochrobactrum anthropi 2/3.
abstract: A biosurfactant-producing bacterium, isolate 2/3, was isolated from mangrove sediment in the south of Thailand. It was evaluated as a potential biosurfactant producer. The highest biosurfactant production (4.52 g/l) was obtained when the cells were grown on a minimal salt medium containing 25 % (v/v) palm oil decanter cake and 1 % (w/v) commercial monosodium glutamate as carbon and nitrogen sources, respectively. After microbial cultivation at 30 °C in an optimized medium for 96 h, the biosurfactant produced was found to reduce the surface tension of pure water to 25.0 mN/m with critical micelle concentrations of 8.0 mg/l. The stability of the biosurfactant at different salinities, pH and temperature and also its emulsifying activity was investigated. It is an effective surfactant at very low concentrations over a wide range of temperatures, pH and salt concentrations. The biosurfactant obtained was confirmed as a glycolipid type biosurfactant by using a biochemical test, fourier-transform infrared spectroscopy, MNR and mass spectrometry. The crude biosurfactant showed a broad spectrum of antimicrobial activity and also had the ability to emulsify oil and enhance polyaromatic hydrocarbons solubility.
authors.affiliation: Faculty of Science and Technology, Suratthani Rajabhat University, Suratthani, 84100, Thailand.
authors.forename: Pongsak
authors.initials: P
authors.lastname: Noparat
authors.forename: Suppasil
authors.initials: S
authors.lastname: Maneerat
authors.forename: Atipan
authors.initials: A
authors.lastname: Saimmai
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D006017:Glycolipids / Q000737:chemistry / Q000378:metabolism; D007220:Industrial Waste; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D020621:Ochrobactrum anthropi / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D000073878:Palm Oil; D010938:Plant Oils; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013500:Surface Tension / Q000187:drug effects; D013501:Surface-Active Agents / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature; D013785:Thailand; D013995:Time; D014867:Water / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006017:Glycolipids; D007220:Industrial Waste; D010938:Plant Oils; D012336:RNA, Ribosomal, 16S; D013501:Surface-Active Agents; D014867:Water; D000073878:Palm Oil; D002244:Carbon; D009584:Nitrogen