title: Screening for MiRNAs related to laryngeal squamous carcinoma stem cell radiation.
abstract: OBJECTIVE
  To use microarray chip technology for screening of stem cell radiation related miRNAs in laryngeal squamous cell carcinoma; study and explore the relationship of miRNAs with radiosensitivity of laryngeal squamous cells.
  
  
  METHODS
  After conventional culture and amplification of the laryngeal squamous carcinoma cell line Hep-2, CD 133+ cells were screened out with combination of isolated culture of stem cell microspheres and FACS for preparation of laryngeal cancer stem cells. After radiation treatment, miRNAs of laryngeal squamous carcinoma stem cells before and after radiation were enriched and purified. After microarray hybridization with mammalian miRNA and scanning of fluorescence signal, the miRNAs of laryngeal squamous carcinoma stem cells before and after radiation was subject to differential screening and clustering analysis. Real-time quantitative RT-PCR was used to verify part of the differentially expressed miRNAs.
  
  
  RESULTS
  70 miRNAs related to laryngeal cancer stem cell radiation with 2-fold difference in expression were screened out, in which 62 were down-regulated and 8 were up-regulated. Fluorescent quantitative RT-PCR results were consistent with miRNAs chip results.
  
  
  CONCLUSIONS
  Some miRNAs may be involved in self-regulation with laryngeal squamous carcinoma stem cell radiation.
authors.affiliation: Department of Oncology, Affiliated Hospital with Hangzhou Normal University School of Medicine, Hangzhou, China E-mail : hcx588@hotmail.com, hcx588@tom.com.
authors.forename: Chang-Xin
authors.initials: CX
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.forename: Guang-Liang
authors.initials: GL
authors.lastname: Duan
authors.forename: Ji-Fen
authors.initials: JF
authors.lastname: Yao
authors.forename: Zhao-Yang
authors.initials: ZY
authors.lastname: Li
authors.forename: Da
authors.initials: D
authors.lastname: Li
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Wang
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000532:radiotherapy; D020869:Gene Expression Profiling; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000532:radiotherapy; D035683:MicroRNAs / Q000235:genetics*; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000528:radiation effects*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D011836:Radiation Tolerance / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D014965:X-Rays
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D012333:RNA, Messenger