title: Fabrication of perfusable vasculatures by using micromolding and electrochemical cell transfer.
abstract: Fabrication of vascular networks for the delivery of oxygen and nutrients is a critical issue when engineering 3-dimensional tissues and organs. This study describes an approach that involves micromolding and electrochemical cell transfer and can be employed to fabricate endothelial cell-lined vascular-like structures that are precisely aligned at micrometer intervals in a photocrosslinkable gelatin hydrogel. Subsequent perfusion culture induces the migration and sprouting of endothelial cells in the hydrogel and facilitates luminal structure formation.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Osaki
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kakegawa
authors.forename: Naoto
authors.initials: N
authors.lastname: Mochizuki
authors.forename: Junji
authors.initials: J
authors.lastname: Fukuda
mesh_terms: D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D055664:Electrochemical Techniques / Q000379:methods*; D005780:Gelatin / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D010539:Permeability; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D020100:Hydrogels; D005780:Gelatin