title: Lighting up cysteine and homocysteine in sequence based on the kinetic difference of the cyclization/addition reaction.
abstract: A novel one- and two-photon fluorescent probe CB1 has been developed for discriminating Cys and Hcy in a successive manner with high selectivity. The discrete time-dependent fluorescent responses enable us to sequentially detect Cys and Hcy in different time windows. Two-step reaction and kinetic modes were used to explain the sensing mechanism. As a promising biosensor for cell imaging, CB1 has been confirmed to exhibit membrane permeability to intact cells, low cytotoxicity to viable cells and photostability to ultraviolet light excitation. Furthermore, the results from the control assay have shown that the one- and two-photon fluorescence of CB1 within cells is associated with intracellular mercapto biomolecules but yet there is little interference with physiological pH value, viscosity and common bioanalytes. Finally one- and two-photon fluorescent images of CB1 within living SiHa cells have been presented.
authors.affiliation: Center of Bio & Micro/Nano Functional Materials, State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, P. R. China. yuxq@sdu.edu.cn.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Guo
authors.forename: Minggang
authors.initials: M
authors.lastname: Tian
authors.forename: Fang
authors.initials: F
authors.lastname: Miao
authors.forename: Weijia
authors.initials: W
authors.lastname: Zhang
authors.forename: Guofen
authors.initials: G
authors.lastname: Song
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Yu
authors.forename: Jing Zhi
authors.initials: JZ
authors.lastname: Sun
authors.forename: Wai-Yeung
authors.initials: WY
authors.lastname: Wong
mesh_terms: D003500:Cyclization; D003545:Cysteine / Q000737:chemistry*; D005453:Fluorescence; D006710:Homocysteine / Q000737:chemistry*; D007700:Kinetics
chemical_list: D006710:Homocysteine; D003545:Cysteine