title: Atypical protein kinase Cλ is critical for growth factor receptor-induced dorsal ruffle turnover and cell migration.
abstract: Gα13, a member of the heterotrimeric G proteins, is critical for actin cytoskeletal reorganization and cell migration. Previously we have shown that Gα13 is essential for both G protein-coupled receptor and receptor tyrosine kinase-induced actin cytoskeletal reorganization such as dynamic dorsal ruffle turnover and cell migration. Ric-8A, a non-receptor guanine nucleotide exchange factor for some heterotrimeric G proteins, is critical for coupling receptor tyrosine kinases to Gα13. Here, we show that PDGF can induce phosphorylation of Ric-8A. Atypical protein kinase Cλ (aPKCλ) is required for Ric-8A phosphorylation. Furthermore, aPKCλ is required for PDGF-induced dorsal ruffle turnover and cell migration as demonstrated by both down-regulation of aPKCλ protein levels in cells by RNA interference and by studies in aPKCλ knock-out cells. Moreover, phosphorylation of Ric-8A modulates its subcellular localization. Hence, aPKCλ is critical for PDGF-induced actin cytoskeletal reorganization and cell migration.
authors.affiliation: From the College of Life Sciences, Wuhan University, Wuhan 430072, China, and.
authors.forename: Bowen
authors.initials: B
authors.lastname: Xing
authors.forename: Limin
authors.initials: L
authors.lastname: Wang
authors.forename: Dagang
authors.initials: D
authors.lastname: Guo
authors.forename: Jianyun
authors.initials: J
authors.lastname: Huang
authors.forename: Cedric
authors.initials: C
authors.lastname: Espenel
authors.forename: Geri
authors.initials: G
authors.lastname: Kreitzer
authors.forename: J Jillian
authors.initials: JJ
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
authors.forename: Xin-Yun
authors.initials: XY
authors.lastname: Huang
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000235:genetics / Q000378:metabolism*; D044385:GTP-Binding Protein alpha Subunits / Q000235:genetics / Q000378:metabolism; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D010766:Phosphorylation / Q000502:physiology; D010982:Platelet-Derived Growth Factor / Q000235:genetics / Q000378:metabolism; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology; D017479:Receptors, Platelet-Derived Growth Factor / Q000235:genetics / Q000378:metabolism*
chemical_list: D000199:Actins; D044385:GTP-Binding Protein alpha Subunits; D020662:Guanine Nucleotide Exchange Factors; D010982:Platelet-Derived Growth Factor; C485884:Ric8a protein, mouse; D017479:Receptors, Platelet-Derived Growth Factor; C115015:PKC-3 protein; D011493:Protein Kinase C
keywords: Cell Biology; Cell Migration; Cell Signaling; Growth Factor Receptor; Growth Factors; Protein Kinase C (PKC); Signal Transduction