title: AZD1480 can inhibit the biological behavior of ovarian cancer SKOV3 cells in vitro.
abstract: OBJECTIVE
  To study the mechanism of effects of AZD1480 on the SKOV3 ovarian cancer cell line.
  
  
  METHODS
  The MTT method was used to assess cellular proliferation, flow cytometry for cellular apoptosis, the scratch test to determine migration, transwell chamber assays to detect cellular invasion, plate clone experiments to detect the clone forming ability and Western blotting to determine p-STAT3 protein levels.
  
  
  RESULTS
  The proliferation rate, migration ability, invasiveness and the clone forming ability of SKOV3 cells were reduced after treatment with AZD1480, while apoptosis rate and chemotherapeutic susceptibility were increased. After treatment with AZD1480 plus cisplatin, the apoptosis rate increased significantly while the expression level of p-STAT3 protein was decreased.
  
  
  CONCLUSIONS
  AZD1480 can inhibit the proliferation, invasion, metastasis and clone formation of SKOV3 cells, induce cellulsar apoptosis, increase the chemotherapeutic sensitivity and reduce the expression level of p-STAT3 protein.
authors.affiliation: Department of Gynecology and Obstetrics, HeBei United University Affiliated Hospital, Tangshan, HeBei, China E-mail : hanpinghp1@126.com.
authors.forename: Zhao-Ling
authors.initials: ZL
authors.lastname: Sun
authors.forename: Ya-Juan
authors.initials: YJ
authors.lastname: Tang
authors.forename: Wei-Guang
authors.initials: WG
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Xing
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: He
authors.forename: De-Mei
authors.initials: DM
authors.lastname: Xin
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Yu
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Ping
authors.initials: P
authors.lastname: Han
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D000971:Antineoplastic Combined Chemotherapy Protocols; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002945:Cisplatin / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D053614:Janus Kinase 2 / Q000037:antagonists & inhibitors; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D011720:Pyrazoles / Q000494:pharmacology*; D011743:Pyrimidines / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: C545606:AZD 1480; D000970:Antineoplastic Agents; D011720:Pyrazoles; D011743:Pyrimidines; D053614:Janus Kinase 2; D002945:Cisplatin