title: Effect of different kinds of anoxia/reoxygenation on the mitochondrial function and the free radicals production of cultured primary equine skeletal myoblasts.
abstract: Horses are outstanding athletes, performing in many different disciplines involving different kinds of efforts and metabolic responses. Depending on exercise intensity, their skeletal muscle oxygenation decreases, and the reperfusion at cessation of the exercise can cause excessive production of free radicals. This study on cultured primary equine myoblasts investigated the effect of different kinds of anoxia/reoxygenation (A/R) on routine respiration, mitochondrial complex I specific activity and free radicals production. Our data revealed that short cycles of A/R caused a decrease of all the parameters, opposite to what a single long period of anoxia did. A preconditioning-like effect could explain our first pattern of results whereas mild uncoupling could be more appropriate for the second one. Anyway, it seems that mitochondrial complex I could play a major role in the regulation of the balance between metabolic and antioxidant protection of the muscular function of athletic horses.
authors.affiliation: Center for Oxygen Research and Development, Institute of Chemistry B6a, University of Liège, Sart Tilman, 4000 Liège, Belgium. Electronic address: j.ceusters@ulg.ac.be.
authors.forename: Justine D
authors.initials: JD
authors.lastname: Ceusters
authors.forename: Ange A
authors.initials: AA
authors.lastname: Mouithys-Mickalad
authors.forename: Thierry J
authors.initials: TJ
authors.lastname: Franck
authors.forename: Ginette P
authors.initials: GP
authors.lastname: Deby-Dupont
authors.forename: Sandrine
authors.initials: S
authors.lastname: Derochette
authors.forename: Didier A
authors.initials: DA
authors.lastname: Serteyn
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D005609:Free Radicals / Q000378:metabolism*; D006736:Horses; D000860:Hypoxia / Q000378:metabolism; D007473:Ion Channels / Q000378:metabolism; D008931:Mitochondria, Muscle / Q000378:metabolism* / Q000502:physiology; D024101:Mitochondrial Proteins / Q000378:metabolism; D032446:Myoblasts / Q000378:metabolism* / Q000502:physiology; D010100:Oxygen / Q000378:metabolism; D000071247:Uncoupling Protein 3
chemical_list: D005609:Free Radicals; D007473:Ion Channels; D024101:Mitochondrial Proteins; D000071247:Uncoupling Protein 3; D000255:Adenosine Triphosphate; D010100:Oxygen
keywords: A/R; Anoxia/reoxygenation; Cultured primary equine skeletal myoblasts; ESR; Mitochondria; OXPHOS; ROS; Reactive oxygen species; UCP; anoxia/reoxygenation; electron spin resonance; oxidative phosphorylation; reactive oxygen species; uncoupling protein