title: Molecule-binding dependent assembly of split aptamer and γ-cyclodextrin: a sensitive excimer signaling approach for aptamer biosensors.
abstract: A highly sensitive and selective fluorescence aptamer biosensors for the determination of adenosine triphosphate (ATP) was developed. Binding of a target with splitting aptamers labeled with pyrene molecules form stable pyrene dimer in the γ-cyclodextrin (γ-CD) cavity, yielding a strong excimer emission. We have found that inclusion of pyrene dimer in γ-cyclodextrin cavity not only exhibits additive increases in quantum yield and emission lifetime of the excimer, but also facilitates target-induced fusion of the splitting aptamers to form the aptamer/target complex. As proof-of-principle, the approach was applied to fluorescence detection of adenosine triphosphate. With an anti-ATP aptamer, the approach exhibits excimer fluorescence response toward ATP with a maximum signal-to-background ratio of 32.1 and remarkably low detection limit of 80 nM ATP in buffer solution. Moreover, due to the additive fluorescence lifetime of excimer induced by γ-cyclodextrin, time-resolved measurements could be conveniently used to detect as low as 0.5 μM ATP in blood serum quantitatively.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China; Hubei Key Laboratory of Mine Environmental Pollution Control & Remediation, Environmental Science and Engineering College, Hubei Polytechnic University, Huangshi 435003, PR China.
authors.forename: Fen
authors.initials: F
authors.lastname: Jin
authors.forename: Yan
authors.initials: Y
authors.lastname: Lian
authors.forename: Jishan
authors.initials: J
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Zheng
authors.forename: Yaping
authors.initials: Y
authors.lastname: Hu
authors.forename: Jinhua
authors.initials: J
authors.lastname: Liu
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.forename: Ronghua
authors.initials: R
authors.lastname: Yang
mesh_terms: D000255:Adenosine Triphosphate / Q000097:blood*; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques; D001774:Blood Chemical Analysis / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D011485:Protein Binding; D015398:Signal Transduction; D047408:gamma-Cyclodextrins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D047408:gamma-Cyclodextrins; D000255:Adenosine Triphosphate; C023792:gamma-cyclodextrin
keywords: Adenosine triphosphate; Fluorescence lifetime; Pyrene dimer; Splitting aptamer; γ-Cyclodextrin