title: Faujasites incorporated tissue engineering scaffolds for wound healing: in vitro and in vivo analysis.
abstract: Exploring the possibility of using inorganic faujasites in tissue engineering scaffolds is a prospective approach in regenerative medicine. Novel gelatin/hyaluronic acid (HA)/faujasite porous scaffolds with low surface energy were fabricated by lyophilization. The pore size of gelatin/HA scaffold was 50-2000 μm, whereas it was greatly reduced to 10-250 μm after incorporation of 2.4% (w/w) of faujasites in polymer matrix, GH(2.4%). Micro computed tomography analysis showed that the porosity of GH(2.4%) was 90.6%. The summative effect was ideal for growth of dermal fibroblasts and cellular attachment. XRD analysis revealed that the embedded faujasites maintained their crystallinity in the polymer matrix even though they interacted with the polymers as indicated by FT-IR analysis. Coupling with effective reinforcement of faujasites, GH(2.4%) demonstrated compression modulus of 929 ± 7 Pa and glass transition temperature of 31 ± 0.05 °C. It exhibited controlled swelling and degradation, allowing sufficient space for tissue regrowth. The latter is further supported by capability of faujasites to provide efficient oxygen supply to fibroblast cells. GH(2.4%) showed a cell viability of 91 ± 8% on NIH 3T3 fibroblast cell lines. The in vivo studies on Sprague-Dawley rats revealed its ability to enhance wound healing by accelerating re-epithelization and collagen deposition. These findings indicated its potential as excellent wound dressing material.
authors.affiliation: Laboratoire Ingénierie des Matériaux de Bretagne, Université de Bretagne Sud , BP 92116, 56321 Lorient Cedex, France.
authors.forename: Neethu
authors.initials: N
authors.lastname: Ninan
authors.forename: Muthunarayanan
authors.initials: M
authors.lastname: Muthiah
authors.forename: In-Kyu
authors.initials: IK
authors.lastname: Park
authors.forename: Anne
authors.initials: A
authors.lastname: Elain
authors.forename: Tin Wui
authors.initials: TW
authors.lastname: Wong
authors.forename: Sabu
authors.initials: S
authors.lastname: Thomas
authors.forename: Yves
authors.initials: Y
authors.lastname: Grohens
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000627:therapeutic use; D003094:Collagen / Q000737:chemistry; D005347:Fibroblasts / Q000187:drug effects; D005780:Gelatin / Q000737:chemistry; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000627:therapeutic use; D011108:Polymers / Q000737:chemistry; D051381:Rats; D044968:Regenerative Medicine; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing; D017641:Zeolites / Q000737:chemistry* / Q000627:therapeutic use
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; C539489:faujasite; D017641:Zeolites; D005780:Gelatin; D006820:Hyaluronic Acid; D003094:Collagen