title: Sirolimus encapsulated liposomes for cancer therapy: physicochemical and mechanical characterization of sirolimus distribution within liposome bilayers.
abstract: Sirolimus has recently been introduced as a therapeutic agent for breast and prostate cancer. In the current study, conventional and Stealth liposomes were used as carriers for the encapsulation of sirolimus. The physicochemical characteristics of the sirolimus liposome nanoparticles were investigated including the particle size, zeta potential, stability and membrane integrity. In addition atomic force microscopy was used to study the morphology, surface roughness and mechanical properties such as elastic modulus deformation and deformation. Sirolimus encapsulation in Stealth liposomes showed a high degree of deformation and lower packing density especially for dipalmitoyl-phosphatidylcholine (DPPC) Stealth liposomes compared to unloaded. Similar results were obtained by differential scanning calorimetry (DSC) studies; sirolimus loaded liposomes were found to result in a distorted state of the bilayer. X-ray photon electron (XPS) analysis revealed a uniform distribution of sirolimus in multilamellar DPPC Stealth liposomes compared to a nonuniform, greater outer layer lamellar distribution in distearoylphosphatidylcholine (DSPC) Stealth liposomes.
authors.affiliation: School of Science, University of Greenwich , Medway Campus, Chatham Maritime, Kent ME4 4TB, U.K.
authors.forename: Ichioma
authors.initials: I
authors.lastname: Onyesom
authors.forename: Dimitrios A
authors.initials: DA
authors.lastname: Lamprou
authors.forename: Lamprini
authors.initials: L
authors.lastname: Sygellou
authors.forename: Samuel K
authors.initials: SK
authors.lastname: Owusu-Ware
authors.forename: Milan
authors.initials: M
authors.lastname: Antonijevic
authors.forename: Babur Z
authors.initials: BZ
authors.lastname: Chowdhry
authors.forename: Dennis
authors.initials: D
authors.lastname: Douroumis
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D008081:Liposomes / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D020123:Sirolimus / Q000737:chemistry*
chemical_list: D008081:Liposomes; D015060:1,2-Dipalmitoylphosphatidylcholine; D020123:Sirolimus