title: Triterpene saponins from the aerial parts of Trifolium medium L. var. sarosiense.
abstract: Seven previously unreported triterpene glycosides (1-7) were isolated from methanol extract of the aerial parts of Trifolium medium var. sarosiense (zigzag clover). Their structures were established by the extensive use of 1D and 2D NMR experiments along with ESI-MS and HRMS analyses. Compounds 1-7 are oleanane derivatives characterized by the presence of a keto group at C-22 of an aglycone and a primary alcoholic function at C-24 and differing functions at C-30. Among these, compounds 1-3 and 6 showed a secondary alcoholic function at C-11, which is methoxylated in compounds 4 and 7. Compound 5 was shown to possess a known aglycone, wistariasapogenol A; however, it is described here for the first time as a saponin constituent of the Trifolium genus. Some aspects of taxonomic classification of zigzag clover are also discussed.
authors.affiliation: Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute , ul. Czartoryskich 8, 24-100 Puławy, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Pawelec
authors.forename: Dariusz
authors.initials: D
authors.lastname: Jędrejek
authors.forename: Mariusz
authors.initials: M
authors.lastname: Kowalczyk
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Pecio
authors.forename: Milena
authors.initials: M
authors.lastname: Masullo
authors.forename: Sonia
authors.initials: S
authors.lastname: Piacente
authors.forename: Francisco A
authors.initials: FA
authors.lastname: Macías
authors.forename: Ana M
authors.initials: AM
authors.lastname: Simonet
authors.forename: Wiesław
authors.initials: W
authors.lastname: Oleszek
authors.forename: Anna
authors.initials: A
authors.lastname: Stochmal
mesh_terms: D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification; D029921:Trifolium / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes