title: Short chain N-acyl homoserine lactone production by soil isolate Burkholderia sp. strain A9.
abstract: In the bacteria kingdom, quorum sensing (QS) is a cell-to-cell communication that relies on the production of and response to specific signaling molecules. In proteobacteria, N-acylhomoserine lactones (AHLs) are the well-studied signaling molecules. The present study aimed to characterize the production of AHL of a bacterial strain A9 isolated from a Malaysian tropical soil. Strain A9 was identified as Burkholderia sp. using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and 16S rDNA nucleotide sequence analysis. AHL production by A9 was detected with two biosensors, namely Chromobacterium violaceum CV026 and Escherichia coli [pSB401]. Thin layer chromatography results showed N-hexanoylhomoserine lactone (C6-HSL) and N-octanoylhomoserine lactone (C8-HSL) production. Unequivocal identification of C6-HSL and C8-HSL was achieved by high resolution triple quadrupole liquid chromatography-mass spectrometry analysis. We have demonstrated that Burkholderia sp. strain A9 produces AHLs that are known to be produced by other Burkholderia spp. with CepI/CepR homologs.
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. kokgan@um.edu.my.
authors.forename: Jian Woon
authors.initials: JW
authors.lastname: Chen
authors.forename: Chong-Lek
authors.initials: CL
authors.lastname: Koh
authors.forename: Choon-Kook
authors.initials: CK
authors.lastname: Sam
authors.forename: Wai-Fong
authors.initials: WF
authors.lastname: Yin
authors.forename: Kok-Gan
authors.initials: KG
authors.lastname: Chan
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000302:isolation & purification; D015374:Biosensing Techniques / Q000379:methods*; D019117:Burkholderia / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism*; D053038:Quorum Sensing / Q000502:physiology*; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: C088386:homoserine lactone; D015107:4-Butyrolactone