title: Plum pox virus and sharka: a model potyvirus and a major disease.
abstract: Plum pox virus (PPV) is a member of the genus Potyvirus in the family Potyviridae. PPV diversity is structured into at least eight monophyletic strains.
  First discovered in Bulgaria, PPV is nowadays present in most of continental Europe (with an endemic status in many central and southern European countries) and has progressively spread to many countries on other continents.
  Typical of potyviruses, the PPV genome is a positive-sense single-stranded RNA (ssRNA), with a protein linked to its 5' end and a 3'-terminal poly A tail. It is encapsidated by a single type of capsid protein (CP) in flexuous rod particles and is translated into a large polyprotein which is proteolytically processed in at least 10 final products: P1, HCPro, P3, 6K1, CI, 6K2, VPg, NIapro, NIb and CP. In addition, P3N-PIPO is predicted to be produced by a translational frameshift.
  PPV causes sharka, the most damaging viral disease of stone fruit trees. It also infects wild and ornamental Prunus trees and has a large experimental host range in herbaceous species. PPV spreads over long distances by uncontrolled movement of plant material, and many species of aphid transmit the virus locally in a nonpersistent manner.
  A few natural sources of resistance to PPV have been found so far in Prunus species, which are being used in classical breeding programmes. Different genetic engineering approaches are being used to generate resistance to PPV, and a transgenic plum, 'HoneySweet', transformed with the viral CP gene, has demonstrated high resistance to PPV in field tests in several countries and has obtained regulatory approval in the USA.
authors.affiliation: Departmento de Genética Molecular de Plantas, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, 28049, Madrid, Spain.
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: García
authors.forename: Miroslav
authors.initials: M
authors.lastname: Glasa
authors.forename: Mariano
authors.initials: M
authors.lastname: Cambra
authors.forename: Thierry
authors.initials: T
authors.lastname: Candresse
mesh_terms: D060467:Disease Resistance / Q000235:genetics / Q000276:immunology; D014644:Genetic Variation; D058507:Host Specificity; D008954:Models, Biological; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000276:immunology / Q000706:statistics & numerical data / Q000821:virology*; D017801:Plum Pox Virus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D027861:Prunus / Q000276:immunology / Q000821:virology*