title: Inhibition of focal adhesion kinase induces apoptosis in human osteosarcoma SAOS-2 cells.
abstract: Focal adhesion kinase (FAK), a non-receptor tyrosine kinase protein, acts as an early modulator of integrin signaling cascade, regulating basic cellular functions. In transformed cells, unopposed FAK signaling has been considered to promote tumor growth, progression, and metastasis. The aim of this study was to assess the role of focal adhesion kinase in human osteosarcoma SAOS-2 cells. SAOS-2 cells were transfected with PGPU6/GFP/shNC, and PGPU6/GFP/FAK-334 (shRNA-334), respectively. Expression of FAK was detected by real-time PCR and western blots. MTT assay was used to examine changes in cell proliferation. Cell apoptosis was analyzed by flow cytometry. The expression of caspase-3,-7,-9 was measured by Western blots. The expression of FAK in SAOS-2 cells significantly decreased in shRNA-334 group contrast to the control group (P < 0.01). Cells proliferation was inhibited by shRNA-334 and shRNA-334 + cisplatin, and the effects were clearly enhanced when cells treated with the anticancer agents. The level of cell apoptosis in shRNA-334 and shRNA-334 + cisplatin group was higher than in the control group (P < 0.01). The current data support evidence that down-regulation of FAK could induce SAOS-2 apoptosis through the caspase-dependent cell death pathway. Inhibition of the kinases may be important for therapies designed to enhance the apoptosis in osteosarcoma.
authors.affiliation: Department of Bone Surgery, First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng street, Harbin, Heilongjiang province, 150001, China.
authors.forename: Jialiang
authors.initials: J
authors.lastname: Wang
authors.forename: Jianing
authors.initials: J
authors.lastname: Zu
authors.forename: Gongping
authors.initials: G
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: Jinglong
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology; D020169:Caspases; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002945:Cisplatin / Q000494:pharmacology; D005434:Flow Cytometry; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering; D015398:Signal Transduction / Q000235:genetics
chemical_list: D034741:RNA, Small Interfering; D051416:Focal Adhesion Protein-Tyrosine Kinases; D020169:Caspases; D002945:Cisplatin