title: Conformational dynamics of individual antibodies using computational docking and AFM.
abstract: Molecular recognition between a receptor and a ligand requires a certain level of flexibility in macromolecules. In this study, we aimed at analyzing the conformational variability of receptors portrayed by monoclonal antibodies that have been individually imaged using atomic force microscopy (AFM). Individual antibodies were chemically coupled to activated mica surface, and they have been imaged using AFM in ambient conditions. The resulting topographical surface of antibodies was used to assemble the three subunits constituting antibodies: two antigen-binding fragments and one crystallizable fragment using a surface-constrained computational docking approach. Reconstructed structures based on 10 individual topographical surfaces of antibodies are presented for which separation and relative orientation of the subunits were measured. When compared with three X-ray structures of antibodies present in the protein data bank database, results indicate that several arrangements of the reconstructed subunits are comparable with those of known structures. Nevertheless, no reconstructed structure superimposes adequately to any particular X-ray structure consequence of the antibody flexibility. We conclude that high-resolution AFM imaging with appropriate computational reconstruction tools is adapted to study the conformational dynamics of large individual macromolecules deposited on mica.
authors.affiliation: CEA, iBEB, Service de Biochimie et Toxicologie Nucléaire, F-30207, Bagnols sur Cèze, France.
authors.forename: Rui C
authors.initials: RC
authors.lastname: Chaves
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Teulon
authors.forename: Michael
authors.initials: M
authors.lastname: Odorico
authors.forename: Pierre
authors.initials: P
authors.lastname: Parot
authors.forename: Shu-Wen W
authors.initials: SW
authors.lastname: Chen
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Pellequer
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D021621:Imaging, Three-Dimensional; D007072:Immunoglobulin D / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry; D018625:Microscopy, Atomic Force; D062105:Molecular Docking Simulation; D011487:Protein Conformation
chemical_list: D000906:Antibodies; D007072:Immunoglobulin D; D007074:Immunoglobulin G
keywords: Immunoglobulin G (IgG); Protein structure reconstruction; atomic force microscopy (AFM); docking; structure dynamics