title: A survey of fish viruses isolated from wild marine fishes from the coastal waters of southern Korea.
abstract: A survey was conducted to investigate viral infection in 253 wild marine fishes harvested in the southern coastal area of Korea from 2010 to 2012. The fish that were captured by local anglers were randomly bought and sampled for virus examination. The samples were tested for presence of virus by virus isolation with FHM, FSP, and BF-2 cells and molecular methods (polymerase chain reaction and sequencing). Of the 253 fish sampled, 9 fish were infected with virus. Aquabirnaviruses (ABVs), Viral hemorrhagic septicemia virus (VHSV), and Red seabream iridovirus (RSIV) were detected in 7, 1, and 1 fish, respectively. Molecular phylogenies demonstrated the detected viruses (ABV, VHSV, and RSIV) were more closely related to viruses reported of the same type from Korea and Japan than from other countries, suggesting these viruses may be indigenous to Korean and Japanese coastal waters.
authors.affiliation: 1Myung-Joo Oh, Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University, Yeosu 550-749, Korea. ohmj@chonnam.ac.kr.
authors.forename: Wi-Sik
authors.initials: WS
authors.lastname: Kim
authors.forename: Shin-Young
authors.initials: SY
authors.lastname: Choi
authors.forename: Do-Hyung
authors.initials: DH
authors.lastname: Kim
authors.forename: Myung-Joo
authors.initials: MJ
authors.lastname: Oh
mesh_terms: D000818:Animals; D001483:Base Sequence; D018059:Birnaviridae / Q000235:genetics / Q000302:isolation & purification*; D002460:Cell Line; D005393:Fish Diseases / Q000453:epidemiology / Q000821:virology*; D005399:Fishes; D017994:Iridovirus / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D029141:Novirhabdovirus / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D056910:Republic of Korea / Q000453:epidemiology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral
keywords: Phylogeny; survey; viral infection; wild marine fishes