title: Deep transcriptome sequencing of wild halophyte rice, Porteresia coarctata, provides novel insights into the salinity and submergence tolerance factors.
abstract: Porteresia coarctata is a wild relative of rice with capability of high salinity and submergence tolerance. The transcriptome analyses of Porteresia can lead to the identification of candidate genes involved in salinity and submergence tolerance. We sequenced the transcriptome of Porteresia under different conditions using Illumina platform and generated about 375 million high-quality reads. After optimized assembly, a total of 152 367 unique transcript sequences with average length of 794 bp were obtained. Many of these sequences might represent fragmented transcripts. Functional annotation revealed the presence of genes involved in diverse cellular processes and 2749 transcription factor (TF)-encoding genes in Porteresia. The differential gene expression analyses identified a total of 15 158 genes involved in salinity and/or submergence response(s). The stress-responsive members of different TF families, including MYB, bHLH, AP2-EREBP, WRKY, bZIP and NAC, were identified. We also revealed key metabolic pathways, including amino acid biosynthesis, hormone biosynthesis, secondary metabolite biosynthesis, carbohydrate metabolism and cell wall structures, involved in stress tolerance in Porteresia. The transcriptome analyses of Porteresia are expected to highlight genes/pathways involved in salinity and submergence tolerance of this halophyte species. The data can serve as a resource for unravelling the underlying mechanism and devising strategies to engineer salinity and submergence tolerance in rice.
authors.affiliation: Functional and Applied Genomics Laboratory, National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, New Delhi 110067, India.
authors.forename: Rohini
authors.initials: R
authors.lastname: Garg
authors.forename: Mohit
authors.initials: M
authors.lastname: Verma
authors.forename: Shashank
authors.initials: S
authors.lastname: Agrawal
authors.forename: Rama
authors.initials: R
authors.lastname: Shankar
authors.forename: Manoj
authors.initials: M
authors.lastname: Majee
authors.forename: Mukesh
authors.initials: M
authors.lastname: Jain
mesh_terms: D063990:Gene Ontology; D059014:High-Throughput Nucleotide Sequencing; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics; D054712:Salinity; D055051:Salt-Tolerant Plants / Q000235:genetics / Q000378:metabolism; D017423:Sequence Analysis, RNA / Q000379:methods; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics; D059467:Transcriptome
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors
keywords: Porteresia; metabolic pathways; salinity tolerance; submergence; transcriptome analysis