title: Septins promote dendrite and axon development by negatively regulating microtubule stability via HDAC6-mediated deacetylation.
abstract: Neurite growth requires two guanine nucleotide-binding protein polymers of tubulins and septins. However, whether and how those cytoskeletal systems are coordinated was unknown. Here we show that the acute knockdown or knockout of the pivotal septin subunit SEPT7 from cerebrocortical neurons impairs their interhemispheric and cerebrospinal axon projections and dendritogenesis in perinatal mice, when the microtubules are severely hyperacetylated. The resulting hyperstabilization and growth retardation of microtubules are demonstrated in vitro. The phenotypic similarity between SEPT7 depletion and the pharmacological inhibition of α-tubulin deacetylase HDAC6 reveals that HDAC6 requires SEPT7 not for its enzymatic activity, but to associate with acetylated α-tubulin. These and other findings indicate that septins provide a physical scaffold for HDAC6 to achieve efficient microtubule deacetylation, thereby negatively regulating microtubule stability to an optimal level for neuritogenesis. Our findings shed light on the mechanisms underlying the HDAC6-mediated coupling of the two ubiquitous cytoskeletal systems during neural development.
authors.affiliation: Division of Biological Sciences, Nagoya University Graduate School of Science, Nagoya 464-8602, Japan.
authors.forename: Natsumi
authors.initials: N
authors.lastname: Ageta-Ishihara
authors.forename: Takaki
authors.initials: T
authors.lastname: Miyata
authors.forename: Chika
authors.initials: C
authors.lastname: Ohshima
authors.forename: Masahiko
authors.initials: M
authors.lastname: Watanabe
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Sato
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hamamura
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Higashiyama
authors.forename: Ralph
authors.initials: R
authors.lastname: Mazitschek
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Bito
authors.forename: Makoto
authors.initials: M
authors.lastname: Kinoshita
mesh_terms: D000107:Acetylation; D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure; D002540:Cerebral Cortex / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism* / Q000648:ultrastructure; D005333:Fetus; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D055786:Gene Knockout Techniques; D000073864:Histone Deacetylase 6; D006655:Histone Deacetylases / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D055495:Neurogenesis / Q000235:genetics; D058112:Septins / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D014404:Tubulin / Q000235:genetics* / Q000378:metabolism
chemical_list: D014404:Tubulin; C499033:Hdac6 protein, mouse; D000073864:Histone Deacetylase 6; D006655:Histone Deacetylases; C512895:Sept7 protein, mouse; D058112:Septins