title: Development of polymorphic microsatellite markers for Dioscorea zingiberensis and cross-amplification in other Dioscorea species.
abstract: Dioscorea zingiberensis C.H. Wright (Dioscoreaceae) is an endemic species in central and southwestern China. In order to study the genetic diversity and population structure of this species, 19 novel polymorphic microsatellite loci were developed using a dual-suppression PCR technique. The number of alleles per locus ranged from 3 to 21, with an average of 9.53. All the markers showed high transferability in cross-species amplification in other species of sect. Stenophora.
authors.affiliation: Jiangsu Provincial Key Laboratory for Plant Ex Situ Conservation, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, China.
authors.forename: Q-Q
authors.initials: QQ
authors.lastname: Yan
authors.forename: X-Q
authors.initials: XQ
authors.lastname: Sun
authors.forename: J-L
authors.initials: JL
authors.lastname: Guo
authors.forename: Y-Y
authors.initials: YY
authors.lastname: Hang
authors.forename: M-M
authors.initials: MM
authors.lastname: Li
mesh_terms: D000483:Alleles; D018744:DNA, Plant / Q000235:genetics; D027742:Dioscorea / Q000145:classification / Q000235:genetics*; D056426:Genetic Loci; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013045:Species Specificity
chemical_list: D018744:DNA, Plant