title: [Effect of plasmid transfected shMRE11 on the drug-resistance of BEL7402/5-FU hepatoma cells].
abstract: OBJECTIVE
  To investigate the impact of silencing meiotic recombination 11 (MRE11) gene by shMRE11 on the chemotherapeutic drug sensitivity and the resistance-associated protein (MRP1) expression on the drug-resistant BEL7402/5-FU hepatoma cells, and to confirm the relationship between MRE11 and drug-resistance of hepatoma cells.
  
  
  METHODS
  shMRE11 was transiently transfected into BEL7402/5-FU cells via cathodolyte liposome transfection method. The silencing efficiency was evaluated by quantitative real-time PCR (qRT-PCR) and Western blotting. MTT assay was used to detect the sensitivity of transfected BEL7402/5-FU to cisplatin (DDP), mitomycin C (MMC), adriamycin (ADM) and 5-FU. qRT-PCR and Western blotting were employed respectively to detect the change in the mRNA and protein levels of drug-resistance protein MRP1 in transfected BEL7402/5-FU.
  
  
  RESULTS
  The qRT-PCR and Western blotting showed that the silencing efficiency of MRE11 mRNA and protein was 78.0% and 56.1%, respectively. Compared with the control group, IC50; of shMRE11 group treated with DDP, MMC, ADM, 5-FU was reduced significantly as shown by MTT assay (P<0.05); qRT-PCR and Western blot analysis of MRP1 showed that its expression significantly decreased at the mRNA and protein levels (P<0.05).
  
  
  CONCLUSIONS
  shMRE11 can effectively improve the sensitivity of BEL7402/5-FU cells to anticancer drugs and down-regulate the expression of multidrug resistance associated protein MRP1.
authors.affiliation: Department of Biochemistry and Molecular Biology, Zunyi Medical College, Zunyi 563003, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Fan
authors.forename: Lei
authors.initials: L
authors.lastname: Geng
authors.forename: Changfu
authors.initials: C
authors.lastname: Li
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000188:drug therapy*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics*; D019008:Drug Resistance, Neoplasm; D005472:Fluorouracil / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy*; D000076228:MRE11 Homologue Protein; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000032:analysis / Q000235:genetics; D010957:Plasmids; D012333:RNA, Messenger / Q000032:analysis; D014162:Transfection
chemical_list: D004268:DNA-Binding Proteins; D005472:Fluorouracil; D000076228:MRE11 Homologue Protein; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D012333:RNA, Messenger; C121766:MRE11 protein, human; C421109:multidrug resistance-associated protein 1