title: "Clickable" LNA/DNA probes for fluorescence sensing of nucleic acids and autoimmune antibodies.
abstract: Herein we describe fluorescent oligonucleotides prepared by click chemistry between novel alkyne-modified locked nucleic acid (LNA) strands and a series of fluorescent azides for homogeneous (all-in-solution) detection of nucleic acids and autoimmune antibodies.
authors.affiliation: Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, DK-5230 Odense M, Denmark. ias@sdu.dk.
authors.forename: Anna S
authors.initials: AS
authors.lastname: Jørgensen
authors.forename: Pankaj
authors.initials: P
authors.lastname: Gupta
authors.forename: Jesper
authors.initials: J
authors.lastname: Wengel
authors.forename: I Kira
authors.initials: IK
authors.lastname: Astakhova
mesh_terms: D000480:Alkynes / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000032:analysis; D001323:Autoantibodies / Q000032:analysis*; D001386:Azides / Q000737:chemistry; D057930:Click Chemistry; D004247:DNA / Q000276:immunology; D015342:DNA Probes; D009696:Nucleic Acids / Q000032:analysis*; D009841:Oligonucleotides / Q000138:chemical synthesis*; D013050:Spectrometry, Fluorescence
chemical_list: D000480:Alkynes; D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D001386:Azides; D015342:DNA Probes; D009696:Nucleic Acids; D009841:Oligonucleotides; C477371:locked nucleic acid; D004247:DNA