title: Evaluation of the responses of MHC class II molecule-expressing cells and macrophages to epoxy resin-based and 4-META-containing, methacrylate resin-based root canal sealers in rat subcutaneous tissue.
abstract: Major histocompatibility complex (MHC) class II molecule-expressing cells and macrophages play a pivotal role in mediating the host tissue response to biomaterials. This study investigated the responses of these cells to epoxy resin-based and 4-META-containing, methacrylate resin-based endodontic sealers (AH Plus and MetaSEAL respectively) in rat connective tissue. Silicone tubes loaded with one of the sealers or solid silicone rods (control) were subcutaneously implanted in male Wistar rats for three time periods of 7, 14, or 28 days. Tissue specimens were immunoperoxidase-stained for MHC class II molecules and CD68 (a general macrophage marker). Results showed that AH Plus-implanted tissue displayed significantly more MHC class II-positive cells than the control at 14 and 28 days, whereas MetaSEAL-implanted tissue showed significantly more CD68-positive cells than both AH Plus-implanted tissue and the control at all time periods. It was concluded that the epoxy resin-based sealer induced the infiltration of MHC class II molecule-expressing cells, whereas 4-META-containing, methacrylate resin-based sealer elicited macrophage infiltration.
authors.affiliation: Division of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Yamanaka
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Shigetani
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Yoshiba
authors.forename: Tomoatsu
authors.initials: T
authors.lastname: Kaneko
authors.forename: Nagako
authors.initials: N
authors.lastname: Yoshiba
authors.forename: Takashi
authors.initials: T
authors.lastname: Okiji
mesh_terms: D000818:Animals; D004853:Epoxy Resins; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D008689:Methacrylates / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials
chemical_list: D004853:Epoxy Resins; D000949:Histocompatibility Antigens Class II; D008689:Methacrylates; D012387:Root Canal Filling Materials; C034139:4-methacryloxyethyltrimellitic acid anhydride