title: Peroxidation due to cryoprotectant treatment is a vital factor for cell survival in Arabidopsis cryopreservation.
abstract: Cryopreservation can be a safe and cost-effective tool for the long-term storage of plant germplasm. In Arabidopsis, the ability to recover from cryogenic treatment was lost as growth progressed. Growth could be restored in 48-h seedlings, whereas 72-h seedlings died after cryogenic treatment. Why seedling age and survival are negatively correlated is an interesting issue. A comparative transcriptomics was performed to screen differentially expressed genes between 48- and 72-h seedlings after exposure to cryoprotectant. Among differentially expressed genes, oxidative stress response genes played important roles in cryoprotectant treatment, and peroxidation was a key factor related to cell survival. Seedlings underwent more peroxidation at 72-h than at 48-h. A comprehensive analysis indicated that peroxidation injured membrane systems leading to photophosphorylation and oxidative phosphorylation damage. Furthermore, the apoptosis-like events were found in cryogenic treatment of Arabidopsis seedlings. 48- and 72-h seedlings underwent different degrees of membrane lipid peroxidation during cryoprotectant treatment, and reducing the injury of oxidative stress was an important factor to successful cryopreservation. This study provided a novel insight of genetic regulatory mechanisms in cryopreservation, and established an excellent model to test and evaluate the effect of exogenous antioxidants and conventional cryoprotectants in plant cryopreservation.
authors.affiliation: Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, No. 800, Rd. Dong Chuan, Shanghai, PR China. Electronic address: renliaqx@gmail.com.
authors.forename: Li
authors.initials: L
authors.lastname: Ren
authors.forename: Di
authors.initials: D
authors.lastname: Zhang
authors.forename: Xiang-Ning
authors.initials: XN
authors.lastname: Jiang
authors.forename: Ying
authors.initials: Y
authors.lastname: Gai
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Wang
authors.forename: Barbara M
authors.initials: BM
authors.lastname: Reed
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Shen
mesh_terms: D000375:Aging; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D003451:Cryoprotective Agents / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D008969:Molecular Sequence Data; D010085:Oxidative Phosphorylation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010785:Photophosphorylation / Q000187:drug effects; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000502:physiology
chemical_list: D000975:Antioxidants; D003451:Cryoprotective Agents; D010940:Plant Proteins; D012333:RNA, Messenger
keywords: AFLPa; Arabidopsis thaliana; BLAST; Cryopreservation; EST; FDA; LN; MDA; MS; Murashige and Skoog [1]; NCBI; National Center for Biotechnology Information; Oxidative stress; PVS; Peroxidation; ROS; TBA; TCA; TDFs; basic local alignment search tool; cDNA-AFLP; expressed sequence tag; fluorescein diacetate; liquid nitrogen; malondialdehyde; mplified fragment length polymorphism; plant vitrification solution; qRT-PCR; quantitative real time-PCR; reactive oxygen species; thiobarbituric acid; transcript-derived fragments; trichloroacetic acid