title: Metallic tip enhanced fluorescence for DNA replication monitoring.
abstract: We have successfully performed localized loop-mediated isothermal reactions of hepatitis B virus (HBV) and hepatitis C virus (HCV) on the apex (50~100 nm) of metallic tips coated with Bst polymerases. The SYBR green molecules binding to the new formed HBV DNA inside the optical near fields were excited by two-photon fluorescence microscopy, and directly imaged in far field. Another reporter primer is used for HCV replication detection. Preliminary results are presented in this manuscript.
authors.forename: Shih-Chung
authors.initials: SC
authors.lastname: Wei
authors.forename: Tsung-Liang
authors.initials: TL
authors.lastname: Chuang
authors.forename: Kung-Bin
authors.initials: KB
authors.lastname: Sung
authors.forename: Hui-Hsin
authors.initials: HH
authors.lastname: Lu
authors.forename: Chii-Wann
authors.initials: CW
authors.lastname: Lin
mesh_terms: D017931:DNA Primers / Q000235:genetics; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D005453:Fluorescence; D016174:Hepacivirus / Q000235:genetics; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D007700:Kinetics; D036641:Microscopy, Fluorescence, Multiphoton; D025202:Molecular Diagnostic Techniques / Q000295:instrumentation*; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation*
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D004800:Enzymes, Immobilized; D004259:DNA-Directed DNA Polymerase