title: Different impact of pretransplant anti-HLA antibodies detected by Luminex in highly sensitized renal transplanted patients.
abstract: It is well know that anti-HLA antibodies are an important obstacle in kidney transplantation. Our aim was to study the clinical impact of pretransplant donor specific anti-HLA antibodies (HLA-DSA), in highly sensitized (HS) patients. We analyzed retrospectively the day-of-transplant sera by Luminex Single Antigen Assay (LSA) in HS patients, and the results were correlated with episodes of humoral and cellular rejection as well as with graft and patient survival. All HS subjects received the same induction therapy and rejection episodes were biopsy proven. Thirteen patients (56.5%) preformed HLA-DSA, and we observed higher incidence of acute rejection in aforementioned patients than in the pre-transplant negatives DSA recipients (77% versus 30%, P = 0.03). The one-year graft survival was significantly reduced in positive pre-transplant HLA-DSA patients (60% versus 100%, P = 0.01 Breslow). The positive predicted value of HLA-DSA in relation to rejection reached 100% if patients lost their previous graft in the first year after transplant. Among anti-HLA antibodies present in patients before transplant, HLA-DSA were significantly associated with high risk of acute humoral and cellular rejection and reduced graft survival in posttransplant outcome. The negative impact of these antibodies was even higher when patients suffered an early loss of the previous transplant.
authors.affiliation: Immunology Department, Hospital Clínico San Carlos, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Martín Lagos s/n, 28040 Madrid, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Pérez-Flores
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Santiago
authors.forename: Natividad
authors.initials: N
authors.lastname: Calvo-Romero
authors.forename: Alberto
authors.initials: A
authors.lastname: Barrientos-Guzmán
authors.forename: Ana Isabel
authors.initials: AI
authors.lastname: Sánchez-Fructuoso
mesh_terms: D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000097:blood / Q000302:isolation & purification*; D005260:Female; D005434:Flow Cytometry; D006084:Graft Rejection / Q000097:blood / Q000276:immunology*; D015234:HLA-A Antigens / Q000097:blood / Q000276:immunology / Q000302:isolation & purification*; D006650:Histocompatibility Testing; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D014019:Tissue Donors
chemical_list: D000888:Antibodies, Anti-Idiotypic; D015234:HLA-A Antigens