title: Overproduction of hyperthermostable β-1,4-endoglucanase from the archaeon Pyrococcus horikoshii by tobacco chloroplast engineering.
abstract: One of the most cost-effective methods of producing industrial enzymes is by the use of transgenic plants. We demonstrated successful high-level expression of a hyperthermostable archaeal β-1,4-endoglucanase in mature tobacco leaves by transformation of chloroplasts by homologous recombination. The active recombinant enzyme was readily recovered not only from fresh but also from dried leaves.
authors.affiliation: Venture Business Laboratory, Ehime University.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Nakahira
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ishikawa
authors.forename: Kunisuke
authors.initials: K
authors.lastname: Tanaka
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Tozawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Shiina
mesh_terms: D002480:Cellulase / Q000096:biosynthesis* / Q000737:chemistry*; D002736:Chloroplasts / Q000235:genetics*; D004795:Enzyme Stability; D005818:Genetic Engineering / Q000379:methods*; D043902:Pyrococcus horikoshii / Q000201:enzymology* / Q000235:genetics; D013696:Temperature; D014026:Nicotiana / Q000166:cytology* / Q000235:genetics
chemical_list: D002480:Cellulase