title: Biocompatibility of silver nanoparticles and silver ions in primary human mesenchymal stem cells and osteoblasts.
abstract: The prevention of implant-related infections is an important issue in medical research. The aim is to exploit the strong antimicrobial effect of silver nanoparticles (AgNP) to develop new antibacterial coatings for implants. However, there is still a serious lack of information on the influence of AgNP on bone metabolism. In the present study we have evaluated the influence of AgNP on cell stress, viability, proliferation and differentiation of primary human mesenchymal stem cells (MSC) and osteoblasts (OB). Finally, cellular uptake of the AgNP was examined. After 21 days impairment of cell viability of MSC and OB occurred at a concentration of 10 μg/g of AgNP. Cytotoxicity and inhibition of proliferation was highly time and dose dependent. No influence on cell differentiation, but an increase in cell stress, was observed. Uptake of AgNP into MSC and OB could be confirmed. In summary, these results demonstrate AgNP-mediated cytotoxicity at higher concentrations. Therefore, a therapeutical window for the application of AgNP in medical products might exist. However, the antibacterial benefits and potential health risks of AgNP need to be weighed in further studies.
authors.affiliation: Laboratory for Experimental Trauma Surgery, Justus-Liebig University Giessen, Schubertstrasse 81, 35392 Giessen, Germany.
authors.forename: Linda
authors.initials: L
authors.lastname: Pauksch
authors.forename: Sonja
authors.initials: S
authors.lastname: Hartmann
authors.forename: Marcus
authors.initials: M
authors.lastname: Rohnke
authors.forename: Gabor
authors.initials: G
authors.lastname: Szalay
authors.forename: Volker
authors.initials: V
authors.lastname: Alt
authors.forename: Reinhard
authors.initials: R
authors.lastname: Schnettler
authors.forename: Katrin S
authors.initials: KS
authors.lastname: Lips
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials / Q000494:pharmacology*; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007477:Ions; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012834:Silver / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001672:Biocompatible Materials; D007477:Ions; D012333:RNA, Messenger; D012834:Silver; D000469:Alkaline Phosphatase
keywords: Bone cells; Nanoparticle uptake; Nanotoxicology; Risk assessment; Silver nanoparticles