title: Serum prolidase activity, oxidative stress, and antioxidant enzyme levels in patients with renal cell carcinoma.
abstract: OBJECTIVE
  Prolidase is a member of the matrix metalloproteinase family. It plays a vital role in collagen turnover, matrix remodeling, and cell growth. Reactive oxygen species (ROS) have been implicated in the pathogenesis of various diseases, including cancers. Oxidative stress can cause tumor angiogenesis and may be carcinogenic. However, the relationship between antioxidant capacity and various cancers has been researched in several clinical trials. In our study, we aimed to identify serum prolidase activity, oxidative stress, and antioxidant enzyme levels in patients with renal tumors and to evaluate their relationships with each other.
  
  
  METHODS
  A total of 37 male patients with renal cell cancer and with a mean age of 56.28 ± 3.1 were included in the study. The control group comprising 36 male patients (mean age 56.31 ± 2.9) was randomly selected among the volunteers. Serum samples for measurement of superoxide dismutase (SOD), glutathione peroxidase (GSHPx), glutathione-S-transferase (GST), malondialdehyde (MDA), glutathione (GSH), and prolidase levels were kept at -20°C until they were used.
  
  
  RESULTS
  Serum prolidase activity and MDA levels were significantly higher in renal cancer patients than in controls (all, p < 0.05), while SOD, GSHPx, and GST levels were significantly lower (p < 0.05).
  
  
  CONCLUSIONS
  Our results indicate that increased prolidase seems to be related to increased oxidative stress along with decreased antioxidant levels in renal cancer.
authors.affiliation: Department of Urology, Yuzuncu Yıl University, Van, Turkey necippirincci@hotmail.com.
authors.forename: Necip
authors.initials: N
authors.lastname: Pirinççi
authors.affiliation: Van Training and Research Hospital, Urology Clinic, Van, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Kaba
authors.affiliation: Department of Urology, Yuzuncu Yıl University, Van, Turkey.
authors.forename: İlhan
authors.initials: İ
authors.lastname: Geçit
authors.affiliation: Department of Urology, Yuzuncu Yıl University, Van, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Güneş
authors.affiliation: Department of Urology, Celal Bayar University, Manısa, Turkey.
authors.forename: Mehmet Bilgehan
authors.initials: MB
authors.lastname: Yüksel
authors.affiliation: Department of Urology, Bozok University, Yozgat, Turkey.
authors.forename: Serhat
authors.initials: S
authors.lastname: Tanık
authors.affiliation: Department of Chemistry, Yuzuncu Yıl University, Van, Turkey.
authors.forename: Ayşe
authors.initials: A
authors.lastname: Arslan
authors.affiliation: Department of Chemistry, Yuzuncu Yıl University, Van, Turkey.
authors.forename: Halit
authors.initials: H
authors.lastname: Demir
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D002292:Carcinoma, Renal Cell / Q000097:blood*; D016022:Case-Control Studies; D004150:Dipeptidases / Q000097:blood*; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D005982:Glutathione Transferase / Q000097:blood; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D004150:Dipeptidases; C022449:proline dipeptidase; D005978:Glutathione
keywords: Glutathione peroxidase; malondialdehyde; prolidase activity; renal cell carcinoma; superoxide dismutase