title: One-step generation of engineered drug-laden poly(lactic-co-glycolic acid) micropatterned with Teflon chips for potential application in tendon restoration.
abstract: Regulating cellular behaviors such as cellular spatial arrangement and cellular phenotype is critical for managing tissue microstructure and biological function for engineered tissue regeneration. We herein pattern drug-laden poly(lactic-co-glycolic acid) (PLGA) into grooves using novel Teflon stamps (that possess excellent properties of resistance to harsh organic solvents and molecular adsorption) for engineered tendon-repair therapeutics. The drug release and biological properties of melatonin-laden PLGA grooved micropatterns are investigated. The results reveal that fibroblasts cultured on the melatonin-laden PLGA groove micropatterns not only display significant cell alignment that mimics the cell behavior in native tendon, but also promote the secretion of a major extracellular matrix in tendon, type I collagen, indicating great potential for the engineering of functional tendon regeneration.
authors.affiliation: WPI Advanced Institute for Materials Research, Tohoku University , 2-1-1 Katahira, Aoba-ku, Sendai, 980-8577, Japan.
authors.forename: Xuetao
authors.initials: X
authors.lastname: Shi
authors.forename: Yihua
authors.initials: Y
authors.lastname: Zhao
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhou
authors.forename: Song
authors.initials: S
authors.lastname: Chen
authors.forename: Hongkai
authors.initials: H
authors.lastname: Wu
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry / Q000627:therapeutic use; D024042:Collagen Type I / Q000096:biosynthesis; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry* / Q000627:therapeutic use; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000627:therapeutic use; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011138:Polytetrafluoroethylene / Q000737:chemistry* / Q000627:therapeutic use; D012038:Regeneration; D013710:Tendons / Q000473:pathology / Q000601:surgery*; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011138:Polytetrafluoroethylene