title: A juvenile mouse pheromone inhibits sexual behaviour through the vomeronasal system.
abstract: Animals display a repertoire of different social behaviours. Appropriate behavioural responses depend on sensory input received during social interactions. In mice, social behaviour is driven by pheromones, chemical signals that encode information related to age, sex and physiological state. However, although mice show different social behaviours towards adults, juveniles and neonates, sensory cues that enable specific recognition of juvenile mice are unknown. Here we describe a juvenile pheromone produced by young mice before puberty, termed exocrine-gland secreting peptide 22 (ESP22). ESP22 is secreted from the lacrimal gland and released into tears of 2- to 3-week-old mice. Upon detection, ESP22 activates high-affinity sensory neurons in the vomeronasal organ, and downstream limbic neurons in the medial amygdala. Recombinant ESP22, painted on mice, exerts a powerful inhibitory effect on adult male mating behaviour, which is abolished in knockout mice lacking TRPC2, a key signalling component of the vomeronasal organ. Furthermore, knockout of TRPC2 or loss of ESP22 production results in increased sexual behaviour of adult males towards juveniles, and sexual responses towards ESP22-deficient juveniles are suppressed by ESP22 painting. Thus, we describe a pheromone of sexually immature mice that controls an innate social behaviour, a response pathway through the accessory olfactory system and a new role for vomeronasal organ signalling in inhibiting sexual behaviour towards young. These findings provide a molecular framework for understanding how a sensory system can regulate behaviour.
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: David M
authors.initials: DM
authors.lastname: Ferrero
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Moeller
authors.forename: Takuya
authors.initials: T
authors.lastname: Osakada
authors.forename: Nao
authors.initials: N
authors.lastname: Horio
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Dheeraj S
authors.initials: DS
authors.lastname: Roy
authors.forename: Annika
authors.initials: A
authors.lastname: Cichy
authors.forename: Marc
authors.initials: M
authors.lastname: Spehr
authors.forename: Kazushige
authors.initials: K
authors.lastname: Touhara
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Liberles
mesh_terms: D000375:Aging; D000679:Amygdala / Q000166:cytology; D000818:Animals; D005260:Female; D007765:Lacrimal Apparatus / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010675:Pheromones / Q000378:metabolism* / Q000494:pharmacology; D011984:Sensory Receptor Cells / Q000378:metabolism; D012726:Sexual Behavior, Animal / Q000187:drug effects; D012741:Sexual Maturation; D050052:TRPC Cation Channels / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D013666:Tears / Q000378:metabolism; D019147:Vomeronasal Organ / Q000166:cytology / Q000378:metabolism*
chemical_list: D010675:Pheromones; D050052:TRPC Cation Channels; C491858:Trpc2 protein, mouse