title: (1)H-NMR spectroscopy revealed Mycobacterium tuberculosis caused abnormal serum metabolic profile of cattle.
abstract: To re-evaluate virulence of Mycobacterium tuberculosis (M. tb) in cattle, we experimentally infected calves with M. tb andMycobacterium bovisvia intratracheal injection at a dose of 2.0×10(7) CFU and observed the animals for 33 weeks. The intradermal tuberculin test and IFN-γin vitro release assay showed that both M. tb and M. bovis induced similar responses. Immunohistochemical staining of pulmonary lymph nodes indicated that the antigen MPB83 of both M. tb and M. bovis were similarly distributed in the tissue samples. Histological examinations showed all of the infected groups exhibited neutrophil infiltration to similar extents. Although the infected cattle did not develop granulomatous inflammation, the metabolic profiles changed significantly, which were characterized by a change in energy production pathways and increased concentrations of N-acetyl glycoproteins. Glycolysis was induced in the infected cattle by decreased glucose and increased lactate content, and enhanced fatty acid β-oxidation was induced by decreased TG content, and decreased gluconeogenesis indicated by the decreased concentration of glucogenic and ketogenic amino acids promoted utilization of substances other than glucose as energy sources. In addition, an increase in acute phase reactive serum glycoproteins, together with neutrophil infiltration and increased of IL-1β production indicated an early inflammatory response before granuloma formation. In conclusion, this study indicated that both M. tb and M.bovis were virulent to cattle. Therefore, it is likely that cattle with M. tb infections would be critical to tuberculosis transmission from cattle to humans. Nuclear magnetic resonance was demonstrated to be an efficient method to systematically evaluate M. tb and M. bovi sinfection in cattle.
authors.affiliation: The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China ; College of Animal Science, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Yingyu
authors.initials: Y
authors.lastname: Chen
authors.forename: Junfang
authors.initials: J
authors.lastname: Wu
authors.forename: Lingling
authors.initials: L
authors.lastname: Tu
authors.forename: Xuekai
authors.initials: X
authors.lastname: Xiong
authors.forename: Xidan
authors.initials: X
authors.lastname: Hu
authors.forename: Jiong
authors.initials: J
authors.lastname: Huang
authors.forename: Zhiguang
authors.initials: Z
authors.lastname: Xu
authors.forename: Xiansong
authors.initials: X
authors.lastname: Zhang
authors.forename: Changmin
authors.initials: C
authors.lastname: Hu
authors.forename: Xueying
authors.initials: X
authors.lastname: Hu
authors.forename: Aizhen
authors.initials: A
authors.lastname: Guo
authors.forename: Yulan
authors.initials: Y
authors.lastname: Wang
authors.forename: Huanchun
authors.initials: H
authors.lastname: Chen
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D016002:Discriminant Analysis; D004734:Energy Metabolism / Q000502:physiology*; D006019:Glycolysis; D006023:Glycoproteins / Q000378:metabolism; D007150:Immunohistochemistry / Q000662:veterinary; D009682:Magnetic Resonance Spectroscopy; D009163:Mycobacterium bovis / Q000378:metabolism* / Q000472:pathogenicity; D009169:Mycobacterium tuberculosis / Q000378:metabolism* / Q000472:pathogenicity; D025341:Principal Component Analysis; D060888:Real-Time Polymerase Chain Reaction; D014380:Tuberculosis, Bovine / Q000378:metabolism* / Q000382:microbiology*; D014774:Virulence
chemical_list: D006023:Glycoproteins