title: Identification of combined conjugation of nabumetone phase I metabolites with glucuronic acid and glycine in minipig biotransformation using coupling high-performance liquid chromatography with electrospray ionization mass spectrometry.
abstract: High-performance liquid chromatography (HPLC) coupled with electrospray ionization mass spectrometry (ESI-MS) was applied for the analysis of nabumetone metabolites during the biotransformation in minipigs. In addition to known phase I metabolites, the identification of phase II metabolites was achieved on the basis of their full-scan mass spectra and subsequent MS(n) analysis using both positive-ion and negative-ion ESI mode. Some phase I metabolites are conjugated with both glucuronide acid and glycine, which is quite unusual type of phase II metabolite not presented so far for nabumetone. These metabolites were found in small intestine content, but they were absent in minipigs urine.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, CZ-53210 Pardubice, Czech Republic. Electronic address: lenka.ceslova@upce.cz.
authors.forename: Lenka
authors.initials: L
authors.lastname: Česlová
authors.forename: Michal
authors.initials: M
authors.lastname: Holčapek
authors.forename: Milan
authors.initials: M
authors.lastname: Nobilis
mesh_terms: D000818:Animals; D001711:Biotransformation; D002074:Butanones / Q000097:blood* / Q000493:pharmacokinetics / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D020723:Glucuronic Acid / Q000737:chemistry*; D005998:Glycine / Q000737:chemistry*; D007421:Intestine, Small / Q000187:drug effects; D007477:Ions; D000077430:Nabumetone; D021241:Spectrometry, Mass, Electrospray Ionization; D013552:Swine; D013556:Swine, Miniature; D053719:Tandem Mass Spectrometry; D014867:Water / Q000737:chemistry
chemical_list: D002074:Butanones; D007477:Ions; D014867:Water; D020723:Glucuronic Acid; D000077430:Nabumetone; D005998:Glycine
keywords: Conjugation with glucuronide acid; Conjugation with glycine; Drug metabolites; HPLC/MS; Tandem mass spectrometry