title: Genetic organization of two types of flounder warm-temperature acclimation-associated 65-kDa protein and their gene expression profiles.
abstract: We isolated and characterized two cDNA clone encoding warm-temperature acclimation-associated 65-kDa proteins (PoWap65-1 and PoWap65-2) from the olive flounder, Paralichthys olivaceus. The deduced amino acid sequences of PoWap65s showed overall identities of 33-73% with other fish Wap65 and mammalian hemopexin-like proteins. The 5'-flanking regions of both PoWap65-encoding genes contained various putative transcriptional elements. While PoWap65-1 and PoWap65-2 were structurally similar, they exhibited highly differential patterns of expression. PoWap65-1 was expressed only in the liver, whereas PoWap65-2 transcripts were detected in a wide range of tissues. The accumulation of PoWap65s mRNA was expressed differentially during development. Expression of them in warm temperatures also differed in flounder embryonic cells. PoWap65-1 was upregulated by temperature stimulation whereas PoWap65-2 was not detected. PoWap65s were highly regulated by Edwardsiella tarda infection and hypoxia. Pathogen challenge induced PoWap65-2 expression in the liver whereas PoWap65-1 was downregulated. Hypoxia induced the expression of both PoWap65s in the liver of juvenile fish.
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute.
authors.forename: Young-Ok
authors.initials: YO
authors.lastname: Kim
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Park
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Moon
authors.forename: Bo-Hye
authors.initials: BH
authors.lastname: Nam
authors.forename: Dong-Gyun
authors.initials: DG
authors.lastname: Kim
authors.forename: Hee Jeong
authors.initials: HJ
authors.lastname: Kong
authors.forename: Woo-Jin
authors.initials: WJ
authors.lastname: Kim
authors.forename: Young-Ju
authors.initials: YJ
authors.lastname: Jee
authors.forename: Sang-Jun
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D000064:Acclimatization / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D005432:Flounder / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D000860:Hypoxia / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009928:Organ Specificity; D010802:Phylogeny; D016415:Sequence Alignment; D017421:Sequence Analysis; D013696:Temperature
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins