title: Serum analysis by 1H nuclear magnetic resonance spectroscopy: a new tool for distinguishing neuromyelitis optica from multiple sclerosis.
abstract: BACKGROUND
  Neuromyelitis optica (NMO) and multiple sclerosis (MS), two inflammatory demyelinating diseases, are characterized by different therapeutic strategies. Currently, the only biological diagnostic tool available to distinguish NMO from MS is the specific serum autoantibody that targets aquaporin 4, but its sensitivity is low.
  
  
  OBJECTIVE
  To assess the diagnostic accuracy of metabolomic biomarker profiles in these two neurological conditions, compared to control patients.
  
  
  METHODS
  We acquired serum spectra (47 MS, 44 NMO and 42 controls) using proton nuclear magnetic resonance ((1)H-NMR) spectroscopy. We used multivariate pattern recognition analysis to identify disease-specific metabolic profiles.
  
  
  RESULTS
  The (1)H-NMR spectroscopic analysis evidenced two metabolites, originating probably from astrocytes, scyllo-inositol and acetate, as promising serum biomarkers of MS and NMO, respectively. In 87.8% of MS patients, scyllo-inositol increased 0.15 to 3-fold, compared to controls and in 74.3% of NMO patients, acetate increased 0.4 to 7-fold, compared to controls. Using these two metabolites simultaneously, we can discriminate MS versus NMO patients (sensitivity, 94.3%; specificity, 90.2%).
  
  
  CONCLUSIONS
  This study demonstrates the potential of (1)H-NMR spectroscopy of serum as a novel, promising analytical tool to discriminate populations of patients affected by NMO or MS.
authors.affiliation: ICube, Université de Strasbourg, France.
authors.forename: F-M
authors.initials: FM
authors.lastname: Moussallieh
authors.forename: K
authors.initials: K
authors.lastname: Elbayed
authors.forename: J B
authors.initials: JB
authors.lastname: Chanson
authors.forename: G
authors.initials: G
authors.lastname: Rudolf
authors.forename: M
authors.initials: M
authors.lastname: Piotto
authors.forename: J
authors.initials: J
authors.lastname: De Seze
authors.forename: I J
authors.initials: IJ
authors.lastname: Namer
mesh_terms: D019342:Acetic Acid / Q000097:blood; D000328:Adult; D001253:Astrocytes / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D003937:Diagnosis, Differential; D016002:Discriminant Analysis; D005260:Female; D006801:Humans; D007294:Inositol / Q000097:blood; D016018:Least-Squares Analysis; D008297:Male; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000097:blood / Q000175:diagnosis*; D015999:Multivariate Analysis; D009471:Neuromyelitis Optica / Q000097:blood / Q000175:diagnosis*; D010363:Pattern Recognition, Automated; D011237:Predictive Value of Tests; D066244:Proton Magnetic Resonance Spectroscopy
chemical_list: D015415:Biomarkers; C009217:scyllitol; D007294:Inositol; D019342:Acetic Acid
keywords: Acetate; biomarkers; metabolomics; multiple sclerosis; neurodegeneration; neuromyelitis optica; nuclear magnetic resonance; scyllo-inositol