title: [Protective effect of anhydroicaritin against peritonitis in mice].
abstract: OBJECTIVE
  To investigate the effect of anhydroicaritin (AHI) against zymosan-induced peritonitis in mice.
  
  
  METHODS
  Peritonitis was induced in mice by intraperitoneal injection of zymosan. All mice were monitored for systemic toxicity and mortality for 13 d after zymosan or saline administration. In another set of experiments, the peritoneal exudates were collected. The leukocyte numbers and the production of inflammatory cytokines (IL-6, IL-10, TNF-α, MCP-1) were determined by flow cytometry. The release of nitric oxide (NO) was measured by a Griess reagent system. The Ca(2+); influx in bone marrow-derived macrophages was recorded by laser scanning confocal microscopy with Fluo4-AM loading. The expression of iNOS was determined by Western blotting.
  
  
  RESULTS
  AHI (4 mg/kg) prolonged survival of peritonitis mice, inhibited massive leukocyte transmigration into the peritoneal cavity, and decreased the overproduction of NO, IL-10, TNF-α, MCP-1 and IL-6. In LPS-stimulated mouse macrophages, AHI (5 μmol/L) pretreatment significantly inhibited the elevation of intracellular Ca(2+);, and markedly decreased iNOS protein expression.
  
  
  CONCLUSIONS
  AHI possesses significant protective effects on the zymosan-induced peritonitis mice, which might be associated with the regulation of Ca(2+); influx in macrophages and iNOS expression.
authors.affiliation: Analytical and Testing Center, Jinan University, Guangzhou 510632, China.
authors.forename: Xinqiang
authors.initials: X
authors.lastname: Lai
authors.forename: Xiuyan
authors.initials: X
authors.lastname: Huang
authors.forename: Yaoying
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000818:Animals; D001578:Benzopyrans / Q000627:therapeutic use*; D002118:Calcium / Q000378:metabolism; D016207:Cytokines / Q000032:analysis; D005260:Female; D007958:Leukocyte Count; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D052247:Nitric Oxide Synthase Type II / Q000032:analysis; D010538:Peritonitis / Q000517:prevention & control*
chemical_list: D001578:Benzopyrans; D016207:Cytokines; C578157:anhydroicaritin; D052247:Nitric Oxide Synthase Type II; D002118:Calcium