title: Measurement by SPR of very low dissociation rates: oxidation-mediated loss of biotin-streptavidin affinity.
abstract: Long-term relationship: biotin labels on RNAs, and possibly other biomacromolecules, are easily oxidized causing a dramatic loss of affinity for streptavidin and adversely affecting the measurement of high-affinity interactions. A new SPR method has been developed for measuring the very low rate-dissociation constants of biotin- and biotin oxide-conjugated RNAs with streptavidin.
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich, Wolfgang-Pauli Strasse 10, 8093 Zürich (Switzerland).
authors.forename: Mario A E
authors.initials: MA
authors.lastname: Rebhan
authors.forename: Andreas
authors.initials: A
authors.lastname: Brunschweiger
authors.forename: Jonathan
authors.initials: J
authors.lastname: Hall
mesh_terms: D001710:Biotin / Q000737:chemistry / Q000378:metabolism*; D061565:Cycloaddition Reaction; D007700:Kinetics; D035683:MicroRNAs / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D011485:Protein Binding; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D019809:Streptavidin / Q000378:metabolism*; D020349:Surface Plasmon Resonance
chemical_list: D035683:MicroRNAs; D012313:RNA; D001710:Biotin; D019809:Streptavidin
keywords: biotin; high-affinity interactions; kinetics; streptavidin; surface plasmon resonance