title: Photoswitchable fluorescent proteins for superresolution fluorescence microscopy circumventing the diffraction limit of light.
abstract: In the last two decades, fluorescent proteins became an indispensable tool to noninvasively label a protein in living cells. The discovery of photoswitchable fluorescent proteins expanded the applications of the fluorescent proteins to techniques such as molecular tracking and highlighting on a microscope. Recently, a new microscopic modality to achieve a superresolution circumventing the diffraction limit of light (photoactivated localization microscopy, PALM) has been developed based on the photoswitchable fluorescent proteins. Here we introduce a basic protocol of PALM through the visualization of actin bundles with superresolution.
authors.affiliation: Department of Chemistry, KU Leuven, Leuven, Belgium.
authors.forename: Susana
authors.initials: S
authors.lastname: Rocha
authors.forename: Herlinde
authors.initials: H
authors.lastname: De Keersmaecker
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uji-i
authors.forename: Johan
authors.initials: J
authors.lastname: Hofkens
authors.forename: Hideaki
authors.initials: H
authors.lastname: Mizuno
mesh_terms: D000199:Actins / Q000648:ultrastructure*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008027:Light; D008856:Microscopy, Fluorescence / Q000379:methods*
chemical_list: D000199:Actins; D005456:Fluorescent Dyes