title: Fine tuning and efficient T cell activation with stimulatory aCD3 nanoarrays.
abstract: Anti-CD3 (aCD3) nanoarrays fabricated by self-assembled nanopatterning combined with site-directed protein immobilization techniques represent a novel T cell stimulatory platform that allows tight control over ligand orientation and surface density. Here, we show that activation of primary human CD4+ T cells, defined by CD69 upregulation, IL-2 production and cell proliferation, correlates with aCD3 density on nanoarrays. Immobilization of aCD3 through nanopatterning had two effects: cell activation was significantly higher on these surfaces than on aCD3-coated plastics and allowed unprecedented fine-tuning of T cell response.
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems , Heisenbergstrasse 3, 70569 Stuttgart, Germany.
authors.forename: Jovana
authors.initials: J
authors.lastname: Matic
authors.forename: Janosch
authors.initials: J
authors.lastname: Deeg
authors.forename: Alexander
authors.initials: A
authors.lastname: Scheffold
authors.forename: Itamar
authors.initials: I
authors.lastname: Goldstein
authors.forename: Joachim P
authors.initials: JP
authors.lastname: Spatz
mesh_terms: D015704:CD4 Antigens / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D049329:Nanostructures; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D015704:CD4 Antigens