title: CD133 expression in normal skin and in epithelial cutaneous tumors.
abstract: BACKGROUND
  Expression of human CD133 (human prominin-1) in cancer cells has been postulated to be a marker of stemness and is considered as a putative marker of cancer stem cells (CSCs). We designed a study to describe the expression pattern of CD133 in normal skin and in epithelial cutaneous neoplasms.
  
  
  METHODS
  The CD133 immunohistochemical expression of forty-three eccrine and apocrine tumors was compared to that observed in other epithelial tumors of the skin. In addition, flow cytometry was used to detect the CD133 expression of four epithelial skin neoplasms, including one porocarcinoma.
  
  
  RESULTS
  CD133 immunoreactivity at the apical or at the apicolateral surface of cells forming glandular structures was observed. Cells from solid areas of benign or malignant tumors were not stained. The porocarcinoma derived culture cells showed a 22% of CD133 positive cells using flow cytometry, while squamous cell carcinoma cultures contained less than 0.1%.
  
  
  CONCLUSIONS
  These observations indicate that CD133 is a specific marker of glandular differentiation that could be included in the diagnostic panel of cutaneous tumors with possible eccrine or apocrine differentiation. However, the use of CD133 expression as a marker of CSCs should be interpreted with caution in experiments of skin.
authors.affiliation: Department of Pathology, University General Hospital, 03003 Albacete, Spain.
authors.forename: S H
authors.initials: SH
authors.lastname: Nam-Cha
authors.forename: R
authors.initials: R
authors.lastname: Serrano-Vargas
authors.forename: E
authors.initials: E
authors.lastname: Escario
authors.forename: J M
authors.initials: JM
authors.lastname: Azaña
authors.forename: R
authors.initials: R
authors.lastname: Calero-Oliver
authors.forename: A G
authors.initials: AG
authors.lastname: Martín
authors.forename: E
authors.initials: E
authors.lastname: Poblet
mesh_terms: D000071916:AC133 Antigen; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000096:biosynthesis* / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002454:Cell Differentiation / Q000235:genetics; D045744:Cell Line, Tumor; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics* / Q000473:pathology; D014411:Neoplastic Stem Cells; D010455:Peptides / Q000235:genetics; D061251:Primary Cell Culture; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides