title: Immune responses of mice immunized by DNA plasmids encoding PCV2 ORF 2 gene, porcine IL-15 or the both.
abstract: Porcine circovirus type 2 (PCV2) is associated with many kinds of diseases including postweaning multisystemic wasting syndrome (PMWS). It affects the immune system of swine and causes huge epidemic losses every year. In our previous study, we provided evidence that DNA plasmid bearing porcine IL-15 (pVAX-pIL-15) might serve as an immune enhancer for DNA plasmid encoding porcine reproductive and respiratory syndrome virus GP5 gene. In this study, PCV2 open reading frame (ORF)2 gene was cloned into the eukaryotic expression vector pVAX, resulting in the plasmid pVAX-PCV2-ORF2. Transient expression of the plasmid in BHK-21 cells could be detected using immunofluorescence assay. Experimental mice were divided into 5 groups and immunized with PBS, pVAX, pVAX-pIL-15, pVAX-PCV2-ORF2 or pVAX-pIL-15 plus pVAX-PCV2-ORF2. The results showed that the mice co-inoculated with pVAX-PCV2-ORF2 plus pVAX-pIL-15 had higher humoral and cellular immune responses than the others. In addition, DNA plasmid bearing PCV2 ORF2 gene had a protective effect against challenge with PCV2 in mice which could be promoted with the utilization of pIL-15.
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Department of Clinic Veterinary Science, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Dong
authors.forename: Jing
authors.initials: J
authors.lastname: Feng
authors.forename: Hai
authors.initials: H
authors.lastname: Lin
authors.forename: Lanxiang
authors.initials: L
authors.lastname: Li
authors.forename: Dingding
authors.initials: D
authors.lastname: Su
authors.forename: Di
authors.initials: D
authors.lastname: Tu
authors.forename: Weijuan
authors.initials: W
authors.lastname: Zhu
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ren
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000235:genetics; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D018173:Circoviridae Infections / Q000517:prevention & control; D017925:Circovirus / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D019409:Interleukin-15 / Q000008:administration & dosage* / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D000956:Antigens, Viral; D019409:Interleukin-15; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines
keywords: DNA vaccine; ORF2 gene; PCV2; Porcine interluekin-15