title: Polycation-functionalized nanoporous silicon particles for gene silencing on breast cancer cells.
abstract: Nanoporous silicon particles (pSi), with a pore size in the range of 20-60 nm, were modified with polyethyleneimine (PEI) to yield pSi-PEI particles, which were subsequently complexed with siRNA. Thus, pSi-PEI/siRNA particles were fabricated, with the PEI/siRNA nanocomplexes mainly anchored inside the nanopore of the pSi particles. These hybrid particles were used as carriers to deliver siRNA to human breast cancer cells. Due to the gradual degradation of the pSi matrix under physiological conditions, the PEI/siRNA nanocomplexes were released from the pore interior in a sustained manner. Physicochemical characterization revealed that the released PEI/siRNA nanocomplexes exhibited well-defined spherical shape and narrow particle size distribution between 15 and 30 nm. Gene knockdown against the ataxia telangiectasia mutated (ATM) cancer gene showed dramatic gene silencing efficacy. Moreover, comprehensive biocompatibility studies were performed for the pSi-PEI/siRNA particles both in vitro and in vivo and demonstrated that the pSi-PEI particles exhibited significantly enhanced biocompatibility. As a consequence, PEI-modified porous silicon particles may have substantial potential as safe and effective siRNA delivery systems.
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX 77030, USA; College of Life Information Science and Instrumental Engineering, Hangzhou Dianzi University, Hangzhou, Zhejiang 310018, China.
authors.forename: Mingzhen
authors.initials: M
authors.lastname: Zhang
authors.forename: Rong
authors.initials: R
authors.lastname: Xu
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Xia
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Gu
authors.forename: Guoting
authors.initials: G
authors.lastname: Qin
authors.forename: Xuewu
authors.initials: X
authors.lastname: Liu
authors.forename: Mauro
authors.initials: M
authors.lastname: Ferrari
authors.forename: Haifa
authors.initials: H
authors.lastname: Shen
mesh_terms: D001672:Biocompatible Materials; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D020868:Gene Silencing; D006801:Humans; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D011073:Polyamines / Q000737:chemistry*; D000071228:Polyelectrolytes; D034741:RNA, Small Interfering / Q000235:genetics; D012825:Silicon / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011073:Polyamines; D000071228:Polyelectrolytes; D034741:RNA, Small Interfering; D012825:Silicon
keywords: Breast cancer; Non-viral gene delivery; Polyplex; Porous silicon particles; siRNA