title: Composites for delivery of therapeutics: combining melt electrospun scaffolds with loaded electrosprayed microparticles.
abstract: A novel strategy is reported to produce biodegradable microfiber-scaffolds layered with high densities of microparticles encapsulating a model protein. Direct electrospraying on highly porous melt electrospun scaffolds provides a reproducible scaffold coating throughout the entire architecture. The burst release of protein is significantly reduced due to the immobilization of microparticles on the surface of the scaffold and release mechanisms are dependent on protein-polymer interactions. The composite scaffolds have a positive biological effect in contact with precursor osteoblast cells up to 18 days in culture. The scaffold design achieved with the techniques presented here endorses these new composite scaffolds as promising templates for growth factor delivery.
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, QLD 4059, Australia. n.bock@qut.edu.au.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Bock
authors.forename: Maria A
authors.initials: MA
authors.lastname: Woodruff
authors.forename: Roland
authors.initials: R
authors.lastname: Steck
authors.forename: Dietmar W
authors.initials: DW
authors.lastname: Hutmacher
authors.forename: Brooke L
authors.initials: BL
authors.lastname: Farrugia
authors.forename: Tim R
authors.initials: TR
authors.lastname: Dargaville
mesh_terms: D000818:Animals; D002460:Cell Line; D016503:Drug Delivery Systems / Q000379:methods*; D019344:Lactic Acid / Q000008:administration & dosage / Q000737:chemistry*; D051379:Mice; D010316:Particle Size; D011100:Polyglycolic Acid / Q000008:administration & dosage / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012709:Serum Albumin; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D012709:Serum Albumin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: drug delivery; electrospraying; microstructures; polymer-drug interactions; tissue engineering