title: HPV genotyping in adenocarcinoma of the uterine cervix in Thailand.
abstract: OBJECTIVE
  To determine the distribution of human papillomavirus (HPV) genotypes in cervical adenocarcinoma in Thailand and to evaluate the clinicopathologic characteristics associated with common HPV genotypes.
  
  
  METHODS
  Formalin-fixed, paraffin-embedded tissues from 150 patients with adenocarcinoma were collected from 4 areas of Thailand. Infection with HPV was detected by nested polymerase chain reaction (PCR) with primers MY09/11 and GP5+/6+. Genotyping was performed using a linear array assay, followed by type-specific PCR targeting the E6/E7 regions of HPV-16, HPV-18, and HPV-52 if the linear array test was negative.
  
  
  RESULTS
  Human papillomavirus DNA was detected in 145 (97%) adenocarcinomas (132 single infections; 11 multiple infections; 2 tumors with undetermined HPV type). Genotype 18 was most common (66%), followed by HPV-16 (30%) and HPV-45 (3%). Infection with only HPV-16 and/or HPV-18 accounted for 88% of the HPV-positive tumors. Patients with HPV-18 infection had a younger age (P=0.009) and higher tumor grade (P<0.001) than patients with HPV-16 infection.
  
  
  CONCLUSIONS
  The HPV detection rate in cervical adenocarcinomas in Thailand is high. The predominant genotype is HPV-18, being twice as common as HPV-16. Genotype variations are associated with patient age and tumor grade. Vaccination against HPV-16/HPV-18 might prevent almost 90% of adenocarcinomas.
authors.affiliation: Department of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Sumalee
authors.initials: S
authors.lastname: Siriaunkgul
authors.forename: Utaiwan
authors.initials: U
authors.lastname: Utaipat
authors.forename: Cheepsumon
authors.initials: C
authors.lastname: Suthipintawong
authors.forename: Kobkul
authors.initials: K
authors.lastname: Tungsinmunkong
authors.forename: Surang
authors.initials: S
authors.lastname: Triratanachat
authors.forename: Surapan
authors.initials: S
authors.lastname: Khunamornpong
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology / Q000821:virology*; D000328:Adult; D000367:Age Factors; D000368:Aged; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D052162:Human papillomavirus 16 / Q000235:genetics; D052161:Human papillomavirus 18 / Q000235:genetics; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000821:virology*; D012189:Retrospective Studies; D013785:Thailand / Q000453:epidemiology; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology*
chemical_list: D004279:DNA, Viral
keywords: Adenocarcinoma; Genotyping; Human papillomavirus; Prevalence; Uterine cervix