title: Endoglucanase and total cellulase from newly isolated Rhizopus oryzae and Trichoderma reesei: production, characterization, and thermal stability.
abstract: A multienzymatic complex production was evaluated, as well as endoglucanase and total cellulase characterization, during solid-state fermentation of rice industry wastes with Rhizopus oryzae CCT 7560 (newly isolated microorganism) and Trichoderma reesei QM 9414 (control). R. oryzae produced enzymes with higher activity at 15 h of fermentation (5.1 and 2.3 U g(-1) to endoglucanase and total cellulase), while T. reesei produced them at 55 h (15.3 and 2.8 U g(-1) to endoglucanase and total cellulase). The optimum temperature for total cellulase and endoglucanase was 60 °C. For Trichoderma and Rhizopus, the optimum pH was 5.0 and 6.0 for total cellulase and 6.0 and 5.0 for endoglucanase, respectively. The enzymes produced by Rhizopus presented higher stability at the temperature range evaluated (25-100 °C); the endoglucanase KM value was 20 times lower than the one found for Trichoderma. The characterization of the cellulolytic enzymes from the fungal species native of rice husk revealed that they can be more efficient than the genetically modified enzymes when rice husk and rice bran are used as substrates.
authors.affiliation: Laboratório de Ciência de Alimentos, Escola de Química e Alimentos, Universidade Federal do Rio Grande - FURG, Rua Eng. Alfredo Huch, 475, Centro, 96201-900, Rio Grande, RS, Brazil, larinekupski@yahoo.com.br.
authors.forename: Larine
authors.initials: L
authors.lastname: Kupski
authors.forename: Fernanda Arnhold
authors.initials: FA
authors.lastname: Pagnussatt
authors.forename: Jaqueline Garda
authors.initials: JG
authors.lastname: Buffon
authors.forename: Eliana Badiale
authors.initials: EB
authors.lastname: Furlong
mesh_terms: D002480:Cellulase / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism*; D004795:Enzyme Stability; D005285:Fermentation; D005818:Genetic Engineering; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007220:Industrial Waste; D007700:Kinetics; D012233:Rhizopus / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature; D013816:Thermodynamics; D014242:Trichoderma / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D007220:Industrial Waste; D002480:Cellulase