title: Intracellular co-delivery of zinc ions and plasmid DNA for enhancing gene transfection activity.
abstract: Zinc ions, methylated poly(1-vinylimidazole) (PVIm-Me) and plasmid DNA (pDNA) have formed ternary complexes for gene delivery. The resulting Zn-PVIm-Me-pDNA complexes have delivered both Zn(2+) ions and pDNA inside cells, leading to the nuclear translocation of the pDNA. By use of the pDNA containing a nuclear protein, NF-κB, binding sequence, the intracellular co-delivery of Zn(2+) ions and pDNA has enhanced gene expression. These results suggest that the intracellular Zn(2+) ions delivered by Zn-PVIm-Me-pDNA complexes activated the NF-κB, enhancing the nuclear translocation of the pDNA. In conclusion, it has been demonstrated that the Zn-PVIm-Me-pDNA complex is capable of enhancing the gene transfection activity by a synergic effect of the PVIm-Me and the co-delivered intracellular Zn(2+) ions.
authors.affiliation: Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan. asayama-shoichiro@tmu.ac.jp.
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Asayama
authors.forename: Kosho
authors.initials: K
authors.lastname: Matsuda
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Negishi
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Kawakami
mesh_terms: D021581:Active Transport, Cell Nucleus; D002467:Cell Nucleus / Q000378:metabolism; D056831:Coordination Complexes / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D007093:Imidazoles / Q000737:chemistry; D042541:Intracellular Space / Q000378:metabolism; D018613:Microscopy, Confocal; D015394:Molecular Structure; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011145:Polyvinyls / Q000737:chemistry; D014162:Transfection / Q000379:methods*; D015032:Zinc / Q000737:chemistry / Q000378:metabolism*
chemical_list: D056831:Coordination Complexes; D007093:Imidazoles; D016328:NF-kappa B; D011145:Polyvinyls; C524090:poly(1-vinylimidazole); D004247:DNA; D015032:Zinc