title: Biomimetic-engineered poly (ethylene glycol) hydrogel for smooth muscle cell migration.
abstract: We report on a biomimetic scaffold as a model system to evaluate smooth muscle cell (SMC) migration in three dimensions. To accomplish this, bio-inert poly (ethylene glycol) (PEG)-based hydrogels were designed as the scaffold substrate. To mimic properties of the extracellular matrix, cell-adhesive peptide (GRGDSP) derived from fibronectin and collagenase-sensitive peptide (GPQGIAGQ) derived from collagen type I were incorporated into the PEG macromer chain. Copolymerization of the biomimetic macromers results in the formation of bioactive PEG hydrogels with cell adhesivity and biodegradability. By utilizing these biomimetic scaffolds, we studied the effect of adhesive ligand concentration, proteolysis, and network cross-linking density on cell migration. Our results showed that three-dimensional SMC migration has a biphasic dependence on adhesive ligand density, and both adhesive and collagenase-sensitive peptides were required for cell migration to occur. Furthermore, network cross-linking density was shown to dramatically influence the behavior of cell migration in the hydrogels.
authors.affiliation: 1 Department of Biomedical Engineering, Case Western Reserve University , Cleveland, Ohio.
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Junmin
authors.initials: J
authors.lastname: Zhu
authors.forename: Kandice
authors.initials: K
authors.lastname: Kottke-Marchant
authors.forename: Roger E
authors.initials: RE
authors.lastname: Marchant
mesh_terms: D000595:Amino Acid Sequence; D040761:Biomimetic Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D003331:Coronary Vessels / Q000166:cytology; D003432:Cross-Linking Reagents / Q000494:pharmacology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D008024:Ligands; D008969:Molecular Sequence Data; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000187:drug effects; D009842:Oligopeptides / Q000494:pharmacology; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology*; D059748:Proteolysis / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D003432:Cross-Linking Reagents; D017077:Culture Media, Conditioned; D008024:Ligands; D009842:Oligopeptides; D010455:Peptides; C437167:poly(ethylene glycol)diacrylate; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; C047981:arginyl-glycyl-aspartic acid