title: Evaluating the effectiveness of gel formulation of irradiated seed lectin Cratylia mollis during bone repair in rats.
abstract: BACKGROUND
  Regeneration corresponds to the replacement of damaged cells with ones that have the same morphology and function. For experimental evaluation of materials that may favor the process of bone healing, defects are created with dimensions that prevent spontaneous regeneration. For the development and use of new drugs, it is necessary to study its effects in vitro, which depends on the formulation, concentration, and rate of irradiation in vivo and the route and frequency of administration; thus, it is possible to characterize the physiological and molecular mechanisms involved in the response and cellular effects.
  
  
  OBJECTIVE
  The objective of this study was to assess the effect of Cramoll-1,4 on the process of bone repair.
  
  
  METHODS
  A formulation of biopharmaceutical lectin Cramoll-1,4 at a concentration of 300 mg/100 mL was applied in a single application via gamma radiation and its effect on the process of bone repair in rats was assessed.
  
  
  RESULTS
  Histologically, it was observed that the bone defect is coated by loose connective tissue rich in fibroblasts, providing a range similar to the thick bone original and competing with site of new bone formation. This prevented direct contact between the formulation and experimental bone tissue, as, despite its proven effectiveness in experiments on the repair of skin lesions, the formulation used did not promote bone stimulation that would have promoted the tissue repair process.
  
  
  CONCLUSIONS
  Because of the direct interference of loose tissue repair that prevented direct contact of the implant with the bone interface, the formulation did not promote bone stimulation.
authors.affiliation: Nuclear Engeering Institute, Laboratory of Nanoradiopharmaceuticals, Rio de Janeiro, Brazil.
authors.forename: Ralph
authors.initials: R
authors.lastname: Santos-Oliveira
authors.forename: Maria Helena Madruga
authors.initials: MH
authors.lastname: Lima-Ribeiro
authors.forename: Ana Maria Dos Anjos
authors.initials: AM
authors.lastname: Carneiro-Leão
authors.forename: Adriana Ferreira
authors.initials: AF
authors.lastname: Cruz
authors.forename: Mauricélia Firmino
authors.initials: MF
authors.lastname: de Santana
authors.forename: Carmelita de Lima Bezerra
authors.initials: Cde L
authors.lastname: Cavalcanti
authors.forename: Nicodemos Teles
authors.initials: NT
authors.lastname: de Pontes Filho
authors.forename: Luana Cassandra Breitenbach Barroso
authors.initials: LC
authors.lastname: Coelho
authors.forename: Maria Tereza
authors.initials: MT
authors.lastname: Dos Santos Correia
keywords: Bone repair; cramoll-1,4; irradiated gel; natural products