title: A novel feeder-free culture system for human pluripotent stem cell culture and induced pluripotent stem cell derivation.
abstract: Correct interactions with extracellular matrix are essential to human pluripotent stem cells (hPSC) to maintain their pluripotent self-renewal capacity during in vitro culture. hPSCs secrete laminin 511/521, one of the most important functional basement membrane components, and they can be maintained on human laminin 511 and 521 in defined culture conditions. However, large-scale production of purified or recombinant laminin 511 and 521 is difficult and expensive. Here we have tested whether a commonly available human choriocarcinoma cell line, JAR, which produces high quantities of laminins, supports the growth of undifferentiated hPSCs. We were able to maintain several human pluripotent stem cell lines on decellularized matrix produced by JAR cells using a defined culture medium. The JAR matrix also supported targeted differentiation of the cells into neuronal and hepatic directions. Importantly, we were able to derive new human induced pluripotent stem cell (hiPSC) lines on JAR matrix and show that adhesion of the early hiPSC colonies to JAR matrix is more efficient than to matrigel. In summary, JAR matrix provides a cost-effective and easy-to-prepare alternative for human pluripotent stem cell culture and differentiation. In addition, this matrix is ideal for the efficient generation of new hiPSC lines.
authors.affiliation: Research Programs Unit, Molecular Neurology and Biomedicum Stem Cell Centre, University of Helsinki, Helsinki, Finland.
authors.forename: Sanna
authors.initials: S
authors.lastname: Vuoristo
authors.forename: Sanna
authors.initials: S
authors.lastname: Toivonen
authors.forename: Jere
authors.initials: J
authors.lastname: Weltner
authors.forename: Milla
authors.initials: M
authors.lastname: Mikkola
authors.forename: Jarkko
authors.initials: J
authors.lastname: Ustinov
authors.forename: Ras
authors.initials: R
authors.lastname: Trokovic
authors.forename: Jaan
authors.initials: J
authors.lastname: Palgi
authors.forename: Riikka
authors.initials: R
authors.lastname: Lund
authors.forename: Timo
authors.initials: T
authors.lastname: Tuuri
authors.forename: Timo
authors.initials: T
authors.lastname: Otonkoski
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002460:Cell Line; D002822:Choriocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D053595:Embryonic Stem Cells; D005109:Extracellular Matrix / Q000378:metabolism; D061252:Feeder Cells; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D007797:Laminin; C551997:laminin-511, human