title: Proteomic-based insight into Malpighian tubules of silkworm Bombyx mori.
abstract: Malpighian tubules (MTs) are highly specific organs of arthropods (Insecta, Myriapoda and Arachnida) for excretion and osmoregulation. In order to highlight the important genes and pathways involved in multi-functions of MTs, we performed a systematic proteomic analysis of silkworm MTs in the present work. Totally, 1,367 proteins were identified by one-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry, and as well as by Trans Proteomic Pipeline (TPP) and Absolute protein expression (APEX) analyses. Forty-one proteins were further identified by two-dimensional gel electrophoresis. Some proteins were revealed to be significantly associated with various metabolic processes, organic solute transport, detoxification and innate immunity. Our results might lay a good foundation for future functional studies of MTs in silkworm and other lepidoptera.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology (Southwest University), Chongqing, China.
authors.forename: Xiao-wu
authors.initials: XW
authors.lastname: Zhong
authors.forename: Yong
authors.initials: Y
authors.lastname: Zou
authors.forename: Shi-ping
authors.initials: SP
authors.lastname: Liu
authors.forename: Qi-ying
authors.initials: QY
authors.lastname: Yi
authors.forename: Cui-mei
authors.initials: CM
authors.lastname: Hu
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Qing-you
authors.initials: QY
authors.lastname: Xia
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
mesh_terms: D000818:Animals; D015153:Blotting, Western; D012831:Bombyx / Q000235:genetics* / Q000378:metabolism; D002853:Chromatography, Liquid; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D008317:Malpighian Tubules / Q000378:metabolism*; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics; D053719:Tandem Mass Spectrometry
chemical_list: D017931:DNA Primers; D020543:Proteome