title: Differentiation of isolated and characterized human dental pulp stem cells and stem cells from human exfoliated deciduous teeth: An in vitro study.
abstract: OBJECTIVE
  Isolation, characterization and differentiation of dental pulp stem cells (DPSCs) and stem cells from exfoliated human deciduous teeth (SHED).
  
  
  METHODS
  The pulp tissue was digested in collagenase and cultured in DMEM Dulbecco's Modified Eagle's Media). The stem cells were identified and isolated. Surface characterization of cells was done with flow cytometer using surface markers. An immuno cytochemistry analysis was done. Differentiation potential was analyzed using various differentiation markers.
  
  
  RESULTS
  Flow cytometry analyses for various CD markers showed similar results for both DPSCs and SHED. The cells showed positive expression for pluripotent, ectodermal and mesodermal markers. Cells differentiated into osteoblasts and adipocytes.
  
  
  CONCLUSIONS
  The study demonstrated that stem cells existed in deciduous and permanent pulp tissue. The stem cells present in pulp tissue can be isolated, cultivated and expanded in vitro. Both DPSCs and SHED show almost a similar expression pattern profile for variety of antigens tested.
authors.affiliation: Ahmedabad Municipal Corporation Dental College, Ahmedabad, Gujarat, India.
authors.forename: Vinay Rao
authors.initials: VR
authors.lastname: Vishwanath
authors.forename: Roopa R
authors.initials: RR
authors.lastname: Nadig
authors.forename: Ramanand
authors.initials: R
authors.lastname: Nadig
authors.forename: Jyothi S
authors.initials: JS
authors.lastname: Prasanna
authors.forename: J
authors.initials: J
authors.lastname: Karthik
authors.forename: Veena S
authors.initials: VS
authors.lastname: Pai
keywords: Dental pulp stem cell; dentin regeneration; pulp regeneration; stem cell; tissue engineering; tooth regeneration