title: In vitro reparative dentin: a biochemical and morphological study.
abstract: In this study, starting from human dental pulp cells cultured in vitro, we simulated reparative dentinogenesis using a medium supplemented with different odontogenic inductors. The differentiation of dental pulp cells in odontoblast-like cells was evaluated by means of staining, and ultramorphological, biochemical and biomolecular methods. Alizarin red staining showed mineral deposition while transmission electron microscopy revealed a synthesis of extracellular matrix fibers during the differentiation process. Biochemical assays demonstrated that the differentiated phenotype expressed odontoblast markers, such as Dentin Matrix Protein 1 (DMP1) and Dentin Sialoprotein (DSP), as well as type I collagen. Quantitative data regarding the mRNA expression of DMP1, DSP and type I collagen were obtained by Real Time PCR. Immunofluorescence data demonstrated the various localizations of DSP and DMP1 during odontoblast differentiation. Based on our results, we obtained odontoblast-like cells which simulated the reparative dentin processes in order to better investigate the mechanism of odontoblast differentiation, and dentin extracellular matrix deposition and mineralization.
authors.affiliation: University of Bologna. mirella.falconi@unibo.it.
authors.forename: G
authors.initials: G
authors.lastname: Teti
authors.forename: V
authors.initials: V
authors.lastname: Salvatore
authors.forename: A
authors.initials: A
authors.lastname: Ruggeri
authors.forename: L
authors.initials: L
authors.lastname: Manzoli
authors.forename: M
authors.initials: M
authors.lastname: Gesi
authors.forename: G
authors.initials: G
authors.lastname: Orsini
authors.forename: M
authors.initials: M
authors.lastname: Falconi
mesh_terms: D000880:Anthraquinones / Q000737:chemistry; D015153:Blotting, Western; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D003804:Dentin / Q000737:chemistry* / Q000166:cytology; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006801:Humans; D046529:Microscopy, Electron, Transmission; D009804:Odontoblasts / Q000166:cytology* / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000378:metabolism; D013194:Staining and Labeling; D000099311:Dentin Sialophosphoprotein
chemical_list: D000880:Anthraquinones; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C513309:DMP1 protein, human; C010078:alizarin