title: ¹H, ¹⁵N, and ¹³C resonance assignment of cerato-populin, a fungal PAMP from Ceratocystis populicola.
abstract: Plant pathogenic fungi secrete several non-catalytic proteins involved in various aspects of the pathogenesis process. Amongst these, cerato-populin (Pop1) produced by Ceratocystis populicola; a protein orthologous of cerato-platanin (CP), the core member of the CP family. These two proteins interact with host and non-host plants. In plane leaves they induce synthesis of phytoalexins, disruption of intercellular and intracellular leaf tissue, cell plasmolysis, programmed cell death, over-expression of defence-related genes, H2O2 and NO production, activation of MAPK cascade and plant resistance. All these features point to CP and Pop1 as defence inducers, though Pop1 shows a reduced efficiency. Pop1/CP similarity is 73%. CD spectroscopy highlights some secondary structure differences between Pop1 and CP. Indeed, the region between the first two cysteines (C20-C57), that in CP includes the β2-strand and it is involved in GlcNAc (N-acetyl-D-glucosamine) interaction, in Pop1 is predicted to be fully disordered.
authors.affiliation: Department of Biomedical, Biotechnological and Translational Sciences (S.Bi.Bi.T.), University of Parma, Parma, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Baroni
authors.forename: Luigia
authors.initials: L
authors.lastname: Pazzagli
authors.forename: Simone
authors.initials: S
authors.lastname: Luti
authors.forename: Aniello
authors.initials: A
authors.lastname: Scala
authors.forename: Federica
authors.initials: F
authors.lastname: Martellini
authors.forename: Lorella
authors.initials: L
authors.lastname: Franzoni
authors.forename: Thelma A
authors.initials: TA
authors.lastname: Pertinhez
authors.forename: Alberto
authors.initials: A
authors.lastname: Spisni
mesh_terms: D001203:Ascomycota; D005656:Fungal Proteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D005656:Fungal Proteins