title: An Intelligent Automation Platform for Rapid Bioprocess Design.
abstract: Bioprocess development is very labor intensive, requiring many experiments to characterize each unit operation in the process sequence to achieve product safety and process efficiency. Recent advances in microscale biochemical engineering have led to automated experimentation. A process design workflow is implemented sequentially in which (1) a liquid-handling system performs high-throughput wet lab experiments, (2) standalone analysis devices detect the data, and (3) specific software is used for data analysis and experiment design given the user's inputs. We report an intelligent automation platform that integrates these three activities to enhance the efficiency of such a workflow. A multiagent intelligent architecture has been developed incorporating agent communication to perform the tasks automatically. The key contribution of this work is the automation of data analysis and experiment design and also the ability to generate scripts to run the experiments automatically, allowing the elimination of human involvement. A first-generation prototype has been established and demonstrated through lysozyme precipitation process design. All procedures in the case study have been fully automated through an intelligent automation platform. The realization of automated data analysis and experiment design, and automated script programming for experimental procedures has the potential to increase lab productivity.
authors.affiliation: Department of Biochemical Engineering, University College London, London, UK.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Wu
authors.affiliation: Department of Biochemical Engineering, University College London, London, UK y.zhou@ucl.ac.uk.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000465:Algorithms; D001331:Automation / Q000295:instrumentation* / Q000379:methods*; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D057166:High-Throughput Screening Assays; D008954:Models, Biological; D009113:Muramidase / Q000032:analysis / Q000378:metabolism; D016571:Neural Networks, Computer; D012984:Software; D012995:Solubility
chemical_list: D009113:Muramidase
keywords: automation; integration; multi-agent system; rapid bioprocess design