title: Complexin-1 enhances the on-rate of vesicle docking via simultaneous SNARE and membrane interactions.
abstract: In synaptic terminals, complexin is thought to have inhibitory and activating roles for spontaneous "mini" release and evoked synchronized neurotransmitter release, respectively. We used single vesicle-vesicle microscopy imaging to study the effect of complexin-1 on the on-rate of docking between vesicles that mimic synaptic vesicles and the plasma membrane. We found that complexin-1 enhances the on-rate of docking of synaptic vesicle mimics containing full-length synaptobrevin-2 and full-length synaptotagmin-1 to plasma membrane-mimicking vesicles containing full-length syntaxin-1A and SNAP-25A. This effect requires the C-terminal domain of complexin-1, which binds to the membrane, the presence of PS in the membrane, and the core region of complexin-1, which binds to the SNARE complex.
authors.affiliation: Departments of Molecular and Cellular Physiology, Neurology and Neurological Sciences, Structural Biology, and Photon Science and Howard Hughes Medical Institute, Stanford University , Stanford, California 94305, United States.
authors.forename: Jiajie
authors.initials: J
authors.lastname: Diao
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Cipriano
authors.forename: Minglei
authors.initials: M
authors.lastname: Zhao
authors.forename: Yunxiang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Sachi
authors.initials: S
authors.lastname: Shah
authors.forename: Mark S
authors.initials: MS
authors.lastname: Padolina
authors.forename: Richard A
authors.initials: RA
authors.lastname: Pfuetzner
authors.forename: Axel T
authors.initials: AT
authors.lastname: Brunger
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000737:chemistry / Q000378:metabolism*; D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000378:metabolism*; D050600:SNARE Proteins / Q000737:chemistry / Q000378:metabolism*; D050863:Synaptotagmin I / Q000737:chemistry / Q000378:metabolism
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D009419:Nerve Tissue Proteins; D050600:SNARE Proteins; D050863:Synaptotagmin I; C095992:complexin I; D002118:Calcium