title: Multimodal fluorescence imaging spectroscopy.
abstract: Multimodal fluorescence imaging is a versatile method that has a wide application range from biological studies to materials science. Typical observables in multimodal fluorescence imaging are intensity, lifetime, excitation, and emission spectra which are recorded at chosen locations at the sample. This chapter describes how to build instrumentation that allows for multimodal fluorescence imaging and explains data analysis procedures for the observables.
authors.affiliation: Nanobiophysics Group, University of Twente, Enschede, The Netherlands.
authors.forename: Martijn H W
authors.initials: MH
authors.lastname: Stopel
authors.forename: Christian
authors.initials: C
authors.lastname: Blum
authors.forename: Vinod
authors.initials: V
authors.lastname: Subramaniam
mesh_terms: D015502:Absorptiometry, Photon; D005453:Fluorescence; D008027:Light; D061848:Optical Imaging / Q000379:methods*; D059828:Plant Cells / Q000648:ultrastructure*; D013050:Spectrometry, Fluorescence / Q000379:methods*