title: Green tea (Camellia sinensis) administration induces expression of immune relevant genes and biochemical parameters in rainbow trout (Oncorhynchus mykiss).
abstract: Present study elucidates the efficacy of green tea (Camellia sinensis) on growth performance, immune and antioxidant systems and cytokine gene expression in rainbow trout tissues. Green tea was supplemented at 20, 100, and 500 mg kg(-1) diet and fed to fish (average weight: 23.5 g) for 35 days. No remarkable changes in growth performance were observed among all test groups. Lower lipid peroxidation product and higher superoxide dismutase activity were noted in fish received the medium dose of green tea. Significant increase in serum bactericidal activity and total protein were recorded in all treatment groups. All doses of green tea up-regulated Interleukin-1β transcription in the spleen, while Interleukin-1β mRNA level decreased significantly in the kidney of low dose of green tea. Interleukin-6 mRNA level was up-regulated in the spleen of high dose of green tea and liver of middle and high doses of green tea. High dose and medium dose of green tea up-regulated the interleukin-8 transcription in the kidney and liver, respectively. Meanwhile, green tea inhibited the production of interleukin-10 in all treatment groups compared with control group. Medium dose of green tea up-regulated tumor necrosis factor-α transcription in all fish tissues, while high dose and low dose of green tea enhanced tumor necrosis factor-α mRNA levels in the kidney and spleen, respectively. Present study suggests that green tea especially at 100 mg kg(-1) feed may effectively enhance the antioxidant system and immune system in rainbow trout.
authors.affiliation: Department of Veterinary, Tabriz Branch, Islamic Azad University, Tabriz, Iran. Electronic address: ns76032@gmail.com.
authors.forename: Shahab
authors.initials: S
authors.lastname: Nootash
authors.forename: Najmeh
authors.initials: N
authors.lastname: Sheikhzadeh
authors.forename: Behzad
authors.initials: B
authors.lastname: Baradaran
authors.forename: Ali Khani
authors.initials: AK
authors.lastname: Oushani
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Maleki Moghadam
authors.forename: Katayoon
authors.initials: K
authors.lastname: Nofouzi
authors.forename: Amir
authors.initials: A
authors.lastname: Monfaredan
authors.forename: Leili
authors.initials: L
authors.lastname: Aghebati
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Zare
authors.forename: Sadigheh
authors.initials: S
authors.lastname: Shabanzadeh
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D028241:Camellia sinensis / Q000737:chemistry*; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation; D007378:Interleukins / Q000235:genetics* / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000254:growth & development / Q000276:immunology / Q000502:physiology*; D009928:Organ Specificity; D018515:Plant Leaves / Q000737:chemistry; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000378:metabolism; D044124:Yersinia ruckeri / Q000502:physiology
chemical_list: D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha
keywords: Biochemical parameter; Cytokines; Gene expression; Green tea; Rainbow trout