title: Shotgun protein profile of human adipose tissue and its changes in relation to systemic amyloidoses.
abstract: In systemic amyloidosis, accumulation of misfolded proteins as extracellular amyloid fibrils in tissues causes severe organ dysfunction, but the molecular events of tissue damage related to amyloid deposition are still largely unknown. Through the use of the MudPIT proteomic approach, comprehensive protein profiles of human amyloid-affected adipose tissue from patients and its control (non-amyloid-affected) counterpart were acquired. Label-free comparison between patients and controls made it possible to highlight differences related to the presence of amyloid, by describing up- and down-represented proteins, connected into interacting networks. In particular, extracellular matrix (ECM), protein folding, lipid metabolism, and mitochondrial functions were among the most affected structural/functional pathways. The reported results, obtained with no a priori hypotheses, represent a significant step forward in the clarification of the molecular mechanisms involved in amyloidoses at tissue level and are the premise for understanding protein misfolding diseases.
authors.affiliation: Institute for Biomedical Technologies (ITB-CNR) , Segrate, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Brambilla
authors.forename: Francesca
authors.initials: F
authors.lastname: Lavatelli
authors.forename: Dario
authors.initials: D
authors.lastname: Di Silvestre
authors.forename: Veronica
authors.initials: V
authors.lastname: Valentini
authors.forename: Giovanni
authors.initials: G
authors.lastname: Palladini
authors.forename: Giampaolo
authors.initials: G
authors.lastname: Merlini
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Mauri
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D000682:Amyloid / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000686:Amyloidosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016022:Case-Control Studies; D002853:Chromatography, Liquid; D005109:Extracellular Matrix / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D006801:Humans; D000075363:Immunoglobulin Light-chain Amyloidosis; D050356:Lipid Metabolism / Q000235:genetics; D024101:Mitochondrial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D058977:Molecular Sequence Annotation; D017510:Protein Folding; D025941:Protein Interaction Mapping; D053719:Tandem Mass Spectrometry
chemical_list: D000682:Amyloid; D024101:Mitochondrial Proteins