title: Investigation of effect of nutritional drink on chemotherapy-induced mucosal injury and tumor growth in an established animal model.
abstract: Chemotherapy-induced mucositis represents a significant burden to quality of life and healthcare costs, and may be improved through enhanced nutritional status. We first determined the safety of two nutritional drinks (plus placebo), and then potential gut protection in tumor-bearing rats in a model of methotrexate-induced mucositis. In study 1, animals were fed one of two test diets (or placebo or control chow pellets) for a total of 60 days and were monitored daily. All diets were found to be safe to administer. In study 2, after seven days of receiving diets, a Dark Agouti Mammary Adenocarcinoma (DAMA) was transplanted subcutaneously. Ten days after starting diets, animals had 2 mg/kg intramuscular methotrexate administered on two consecutive days; after this time, all animals were given soaked chow. Animals were monitored daily for changes in bodyweight, tumor burden and general health. Animals were killed 10, 12 and 16 days after initially starting diets, and tissues were collected at necropsy. In study 1, animals receiving diets had gained 0.8% and 10.8% of their starting bodyweight after 60 days, placebo animals 4.4%, and animals fed on standard chow had gained 15.1%. In study 2, there was no significant influence of test diet on bodyweight, organ weight, tumor burden or biochemical parameters. Only animals treated with MTX exhibited diarrhea, although animals receiving Diet A and Diet C showed a non-significant increase in incidence of diarrhea. Administration of these nutritional drinks did not improve symptoms of mucositis.
authors.affiliation: Mucositis Research Group, Centre for Personalised Cancer Medicine (CPCM), Centre for Clinical Research Excellence (CCRE) in Oral Health, Faculty of Health Sciences, University of Adelaide, Frome Road, Adelaide, SA 5005, Australia. emma.bateman@adelaide.edu.au.
authors.forename: Emma
authors.initials: E
authors.lastname: Bateman
authors.forename: Joanne
authors.initials: J
authors.lastname: Bowen
authors.forename: Andrea
authors.initials: A
authors.lastname: Stringer
authors.forename: Bronwen
authors.initials: B
authors.lastname: Mayo
authors.forename: Erin
authors.initials: E
authors.lastname: Plews
authors.forename: Anthony
authors.initials: A
authors.lastname: Wignall
authors.forename: Norman
authors.initials: N
authors.lastname: Greenberg
authors.forename: Eduardo
authors.initials: E
authors.lastname: Schiffrin
authors.forename: Dorothy
authors.initials: D
authors.lastname: Keefe
mesh_terms: D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage / Q000009:adverse effects*; D017209:Apoptosis / Q000187:drug effects; D001628:Beverages; D001835:Body Weight; D049109:Cell Proliferation / Q000187:drug effects; D003967:Diarrhea / Q000139:chemically induced / Q000517:prevention & control; D004032:Diet; D004195:Disease Models, Animal; D005260:Female; D020397:Goblet Cells / Q000187:drug effects / Q000473:pathology; D008727:Methotrexate / Q000008:administration & dosage / Q000009:adverse effects; D052016:Mucositis / Q000139:chemically induced / Q000517:prevention & control*; D009369:Neoplasms / Q000188:drug therapy*; D009752:Nutritional Status; D011897:Random Allocation; D051381:Rats
chemical_list: D000964:Antimetabolites, Antineoplastic; D008727:Methotrexate