title: TFF1 is differentially expressed in stationary and migratory rat gastric epithelial cells (RGM-1) after in vitro wounding: influence of TFF1 RNA interference on cell migration.
abstract: OBJECTIVE
  The trefoil factor family (TFF) peptide TFF1 is typically secreted by gastric surface mucous cells. These cells are also the major players in gastric restitution, i.e., repair of the stomach mucosa by cell migration after injury.
  
  
  METHODS
  An established in vitro model of gastric restitution, i.e., migration of the non-transformed rat gastric cell line RGM-1 after scratch wounding, was investigated by expression profiling of selected genes from separated stationary and migratory cells. Also semi-quantitative immunocytochemistry was performed. Furthermore, RGM-1 cells were transfected with stealth RNAi™ duplexes targeting Tff1 and relative cell migration rates were analyzed.
  
  
  RESULTS
  Surprisingly, Tff1 expression was up-regulated in migratory cells. No unequivocal signs of the epithelial-mesenchymal transition were detectable in migratory cells. Transfection of RGM-1 cells with Tff1-siRNAi duplexes negatively influenced migration of these cells.
  
  
  CONCLUSIONS
  This clearly points to a function of Tff1 as a motogen. A possible up-regulation of TFF1 synthesis in migratory surface mucous cells also in vivo would be an ideal mechanism to specifically enhance gastric restitution only where topologically needed and to minimize eventual negative side effects of TFF1 such as cell scattering and invasiveness.
authors.affiliation: Institute of Molecular Biology and Medicinal Chemistry, Otto-von-Guericke-University Magdeburg, Germany.
authors.forename: Ting
authors.initials: T
authors.lastname: Fu
authors.forename: Hubert
authors.initials: H
authors.lastname: Kalbacher
authors.forename: Werner
authors.initials: W
authors.lastname: Hoffmann
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism*; D005753:Gastric Mucosa / Q000166:cytology*; D007150:Immunohistochemistry; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D014945:Wound Healing / Q000235:genetics / Q000502:physiology
chemical_list: D010455:Peptides