title: Isotope labeling-based quantitative proteomics of developing seeds of castor oil seed (Ricinus communis L.).
abstract: In this study, we used a mass spectrometry-based quantification approach employing isotopic (ICPL) and isobaric (iTRAQ) labeling to investigate the pattern of protein deposition during castor oil seed (Ricinus communis L.) development, including that of proteins involved in fatty acid metabolism, seed-storage proteins (SSPs), toxins, and allergens. Additionally, we have used off-line hydrophilic interaction chromatography (HILIC) as a step of peptide fractionation preceding the reverse-phase nanoLC coupled to a LTQ Orbitrap. We were able to identify a total of 1875 proteins, and from these 1748 could be mapped to extant castor gene models, considerably expanding the number of proteins so far identified from developing castor seeds. Cluster validation and statistical analysis resulted in 975 protein trend patterns and the relative abundance of 618 proteins. The results presented in this work give important insights into certain aspects of the biology of castor oil seed development such as carbon flow, anabolism, and catabolism of fatty acid and the pattern of deposition of SSPs, toxins, and allergens such as ricin and 2S albumins. We also found, for the first time, some genes of SSP that are differentially expressed during seed development.
authors.affiliation: Proteomic Unit, Institute of Chemistry, Universidade Federal do Rio de Janeiro , Av. Athos da Silveira Ramos, 149 - CT-Bloco A, Lab 543, Rio de Janeiro 21941-909, Brazil.
authors.forename: Fábio C S
authors.initials: FC
authors.lastname: Nogueira
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Palmisano
authors.forename: Veit
authors.initials: V
authors.lastname: Schwämmle
authors.forename: Emanuela L
authors.initials: EL
authors.lastname: Soares
authors.forename: Arlete A
authors.initials: AA
authors.lastname: Soares
authors.forename: Peter
authors.initials: P
authors.lastname: Roepstorff
authors.forename: Gilberto B
authors.initials: GB
authors.lastname: Domont
authors.forename: Francisco A P
authors.initials: FA
authors.lastname: Campos
mesh_terms: D016000:Cluster Analysis; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D007553:Isotope Labeling / Q000379:methods; D013058:Mass Spectrometry; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D012278:Ricinus / Q000737:chemistry* / Q000254:growth & development; D055314:Seed Storage Proteins / Q000378:metabolism; D012639:Seeds / Q000737:chemistry* / Q000254:growth & development
chemical_list: D010940:Plant Proteins; D055314:Seed Storage Proteins