title: Bacillus sp. strain P38: an efficient producer of L-lactate from cellulosic hydrolysate, with high tolerance for 2-furfural.
abstract: In this study, efficient polymer-grade L-lactic acid production was achieved with the strain Bacillus sp. P38 by using cellulosic hydrolysate as the sole carbon source. In fed-batch fermentation, 180 g L(-1)L-lactic acid was obtained with a volumetric productivity of 2.4 g L(-1)h(-1) and a yield of 0.96 g g(-1) total reducing sugars. No D-isomer of lactic acid was detected in the broth. Strain P38 tolerated up to 10 g L(-1) 2-furfural, and lactate production was sharply inhibited only when the 2-furfural concentration was higher than 6 g L(-1). Moreover, strain P38 also tolerated high concentrations (>6 g L(-1)) of other fermentation inhibitors in cellulosic hydrolysate, such as vanillin and acetic acid, although it was slightly sensitive to formic acid. The efficient L-lactic acid production, combined with high inhibitor tolerance and efficient pentose utilization, indicate that Bacillus sp. P38 is a promising producer of polymer-grade L-lactic acid from cellulosic biomass.
authors.affiliation: CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; College of Life Science, Qufu Normal University, Qufu 273165, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Peng
authors.forename: Limin
authors.initials: L
authors.lastname: Wang
authors.forename: Chengchuan
authors.initials: C
authors.lastname: Che
authors.forename: Ge
authors.initials: G
authors.lastname: Yang
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.forename: Yanhe
authors.initials: Y
authors.lastname: Ma
mesh_terms: D001407:Bacillus / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D002482:Cellulose / Q000378:metabolism*; D005285:Fermentation; D005662:Furaldehyde / Q000494:pharmacology*; D006868:Hydrolysis / Q000187:drug effects; D019344:Lactic Acid / Q000096:biosynthesis*; D013696:Temperature; D014866:Waste Products / Q000032:analysis; D003313:Zea mays / Q000737:chemistry
chemical_list: D014866:Waste Products; D019344:Lactic Acid; D002482:Cellulose; D005662:Furaldehyde
keywords: 2-Furfural; Bacillus sp.; Cellulosic hydrolysate; l-Lactic acid