title: RNA-seq analysis of antibiotic-producing Bacillus subtilis SC-8 in response to signal peptide PapR of Bacillus cereus.
abstract: Bacillus subtilis SC-8 produces an antibiotic that has narrow antagonistic activity against bacteria in the Bacillus cereus group. In B. cereus group bacteria, peptide-activating PlcR (PapR) plays a significant role in regulating the transcription of virulence factors. When B. subtilis SC-8 and B. cereus are co-cultured, PapR is assumed to stimulate antibiotic production by B. subtilis SC-8. To better understand the effect of PapR on this interspecies interaction, the global transcriptome profile of B. subtilis SC-8 was analyzed in the presence of PapR. Significant changes were detected in 12.8 % of the total transcripts. Genes related to amino acid transport and metabolism (16.5 %) and transcription (15 %) were mainly upregulated, whereas genes involved in carbohydrate transport and metabolism (12.7 %) were markedly downregulated. The expression of genes related to transcription, including several transcriptional regulators and proteins involved in tRNA biosynthesis, was increased. The expression levels of genes associated with several transport systems, such as antibiotic, cobalt, and iron complex transporters, was also significantly altered. Among the downregulated genes were transcripts associated with spore formation, the subtilosin A gene cluster, and nitrogen metabolism.
authors.affiliation: Department of Systems Biotechnology (BK21 program), Chung-Ang University, An-seong, 456-756, Republic of Korea.
authors.forename: In-Cheol
authors.initials: IC
authors.lastname: Yeo
authors.forename: Nam Keun
authors.initials: NK
authors.lastname: Lee
authors.forename: Byung Wook
authors.initials: BW
authors.lastname: Yang
authors.forename: Young Tae
authors.initials: YT
authors.lastname: Hahm
mesh_terms: D000596:Amino Acids / Q000096:biosynthesis; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000494:pharmacology*; D001430:Bacteriocins / Q000096:biosynthesis; D053898:Biosynthetic Pathways / Q000187:drug effects / Q000235:genetics; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D005810:Multigene Family; D021382:Protein Sorting Signals; D012329:RNA, Bacterial / Q000096:biosynthesis; D012343:RNA, Transfer / Q000096:biosynthesis; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D013171:Spores, Bacterial / Q000187:drug effects / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: D000596:Amino Acids; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001430:Bacteriocins; C466079:PapR protein, Bacillus cereus; D021382:Protein Sorting Signals; D012329:RNA, Bacterial; D014157:Transcription Factors; D012343:RNA, Transfer