title: Simultaneous detection of seven sexually transmitted agents in human immunodeficiency virus-infected Brazilian women by multiplex polymerase chain reaction.
abstract: We determined the prevalence of seven clinically important pathogens that cause sexually transmitted infections (STIs) (Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, herpes simplex virus 1 [HSV-1], HSV-2, and Treponema pallidum), by using a multiplex polymerase chain reaction (M-PCR) in samples from Brazilian woman infected with human immunodeficiency virus 1 (HIV-1) and uninfected Brazilian women (controls). The M-PCR assay identified all STIs tested for and surprisingly, occurred association between the control and STIs. This association was probably caused by excellent HIV infection control and regular monitoring in these women established by public health strategies in Brazil to combat HIV/acquired immunodeficiency syndrome. Studies using this M-PCR in different populations may help to better elucidate the roles of STIs in several conditions.
authors.affiliation: Departments of Clinical Analysis and Biomedicine, Statistics, Nursing and Medicine, Universidade Estadual de Maringá, Paraná, Brazil; College of Medicine, Departments of Pharmacology and Medicine, University of Illinois at Chicago, Chicago, Illinois.
authors.forename: Raquel P
authors.initials: RP
authors.lastname: Souza
authors.forename: André L P
authors.initials: AL
authors.lastname: de Abreu
authors.forename: Érika C
authors.initials: ÉC
authors.lastname: Ferreira
authors.forename: Sheila C
authors.initials: SC
authors.lastname: Rocha-Brischiliari
authors.forename: Maria D
authors.initials: MD
authors.lastname: de B Carvalho
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Pelloso
authors.forename: Marcelo G
authors.initials: MG
authors.lastname: Bonini
authors.forename: Fabrícia
authors.initials: F
authors.lastname: Gimenes
authors.forename: Marcia E L
authors.initials: ME
authors.lastname: Consolaro
mesh_terms: D000328:Adult; D001938:Brazil / Q000453:epidemiology; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers / Q000235:genetics; D005260:Female; D015658:HIV Infections / Q000150:complications / Q000453:epidemiology*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D045704:Mycoplasma genitalium / Q000235:genetics / Q000302:isolation & purification; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification; D015995:Prevalence; D012749:Sexually Transmitted Diseases / Q000175:diagnosis* / Q000453:epidemiology; D014210:Treponema pallidum / Q000235:genetics / Q000302:isolation & purification; D014246:Trichomonas vaginalis / Q000235:genetics / Q000302:isolation & purification
chemical_list: D017931:DNA Primers