title: Differential influences of various arsenic compounds on antioxidant defense system in liver and kidney of rats.
abstract: In this study, oxidative stress-related parameters and As retention were examined in liver and kidneys of male Wistar rats exposed to arsenic trioxide, sodium arsenite (iAsIII), sodium arsenate (iAsV), and dimethylarsinic acid (DMAsV) at a single ip dose of 3.8 mgAs/kgbw, at 24h post-exposure. In liver, lipid peroxidation increased in iAsIII-exposed rats, glutathione peroxidase activity decreased in inorganic arsenic (iAs)-exposed rats, and catalase and thioredoxin reductase activities decreased significantly in all As-exposed groups. Both As(III) and As(V) exposure elevated GSH level with no effect on glutathione reductase activity. In kidneys, catalase activity decreased significantly in iAs-exposed, rats; GSH level, glutathione reductase and thioredoxin reductase activity decreased in DMAsV-treated, rats. The tissue As retention was higher in kidneys compared to liver and was also higher in As(III)-exposed compared to As(V)-exposed rats. The results demonstrate similar potency of inorganic As(III) and As(V) compounds to inhibit/induce antioxidant defense system, with liver being more vulnerable to acute As(III)- and As(V)-induced oxidative stress.
authors.affiliation: Department of Pharmacology and Toxicology, Charles University in Prague, Faculty of Medicine in Pilsen, Czech Republic. Electronic address: dana.kotyzova@lfp.cuni.cz.
authors.forename: Dana
authors.initials: D
authors.lastname: Kotyzová
authors.forename: Monika
authors.initials: M
authors.lastname: Bludovská
authors.forename: Vladislav
authors.initials: V
authors.lastname: Eybl
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001151:Arsenic / Q000032:analysis; D001152:Arsenicals; D005978:Glutathione / Q000378:metabolism; D007202:Indicators and Reagents; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000494:pharmacology; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism; D013879:Thioredoxins / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001152:Arsenicals; D007202:Indicators and Reagents; D013879:Thioredoxins; D013880:Thioredoxin-Disulfide Reductase; D005978:Glutathione; D012643:Selenium; D001151:Arsenic
keywords: Arsenate; Arsenic trioxide; Arsenite; Dimethylarsinic acid; Oxidative stress; Selenoenzymes