title: Use of surface-enhanced Raman scattering for detection of cancer-related serum-constituents in gastrointestinal cancer patients.
abstract: Laser-mediated surface-enhanced Raman scattering (SERS) has industrial and biological applications. We have developed a rapid and simple method for generating silver nanoscale hexagonal columns (NHCs) on the surface of a phosphor bronze chip for measurement of SERS spectra. This was used to detect SERS spectra from blood samples obtained from patients with gastric cancer, colorectal cancer, or benign diseases (n=12 each) using a low intensity helium-neon red laser beam with a 632.8-nm wavelength; the intensity of the SERS spectra was compared among the patient groups. The peak heights of SERS spectra from patients with benign diseases were significantly lower than those from patients with gastric or colorectal cancer, whereas those from patients with gastric cancer and colorectal cancer did not differ significantly. Thus, SERS using NHC chips holds promise for the easier and faster detection of cancer-related serum-constituents as biomarkers.
  
  
  BACKGROUND
  In this study, laser-mediated surface-enhanced Raman scattering (SERS) was utilized as a sensitive detection method of cancer-related serum-constituents in gastric and colorectal cancer, via the use of silver nanoscale hexagonal columns on the surface of a phosphor bronze chip for measurement of SERS spectra, paving the way to the development of a rapid and high throughput tool for cancer screening and therapy monitoring.
authors.affiliation: Digestive Disease Center, Showa University Northern Yokohama Hospital, Yokohama, Japan. Electronic address: h.ito@med.showa-u.ac.jp.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ito
authors.affiliation: Digestive Disease Center, Showa University Northern Yokohama Hospital, Yokohama, Japan.
authors.forename: Haruhiro
authors.initials: H
authors.lastname: Inoue
authors.affiliation: Mytech Inc., Kobe, Japan.
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Hasegawa
authors.affiliation: Mytech Inc., Kobe, Japan.
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Hasegawa
authors.affiliation: Lambda Vision Inc., Kanagawa, Japan.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Shimizu
authors.affiliation: Department of Laboratory Medicine and Central Clinical Laboratory, Showa University Northern Yokohama Hospital, Yokohama, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kimura
authors.affiliation: Digestive Disease Center, Showa University Northern Yokohama Hospital, Yokohama, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Onimaru
authors.affiliation: Digestive Disease Center, Showa University Northern Yokohama Hospital, Yokohama, Japan.
authors.forename: Haruo
authors.initials: H
authors.lastname: Ikeda
authors.affiliation: Digestive Disease Center, Showa University Northern Yokohama Hospital, Yokohama, Japan.
authors.forename: Shin-ei
authors.initials: SE
authors.lastname: Kudo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000097:blood / Q000175:diagnosis; D055088:Early Detection of Cancer / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000097:blood / Q000175:diagnosis; D006801:Humans; D007834:Lasers; D008297:Male; D008875:Middle Aged; D049329:Nanostructures / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods; D013274:Stomach Neoplasms / Q000097:blood / Q000175:diagnosis; D013499:Surface Properties
chemical_list: D012834:Silver
keywords: Biomarker; Cancer screening; Nanoscale hexagonal columns; Proteomics; Surface-enhanced Raman scattering