title: Evaluation of bacterial expansin EXLX1 as a cellulase synergist for the saccharification of lignocellulosic Agro-industrial wastes.
abstract: Various types of lignocellulosic wastes extensively used in biofuel production were provided to assess the potential of EXLX1 as a cellulase synergist. Enzymatic hydrolysis of natural wheat straw showed that all the treatments using mixtures of cellulase and an optimized amount of EXLX1, released greater quantities of sugars than those using cellulase alone, regardless of cellulase dosage and incubation time. EXLX1 exhibited different synergism and binding characteristics for different wastes, but this can be related to their lignocellulosic components. The cellulose proportion could be one of the important factors. However, when the cellulose proportion of different biomass samples exhibited no remarkable differences, a higher synergism of EXLX1 is prone to occur on these materials, with a high proportion of hemicellulose and a low proportion of lignin. The information could be favorable to assess whether EXLX1 is effective as a cellulase synergist for the hydrolysis of the used materials. Binding assay experiments further suggested that EXLX1 bound preferentially to alkali pretreated materials, as opposed to acid pretreated materials under the assay condition and the binding preference would be affected by incubation temperature.
authors.affiliation: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, China ; Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Lin
authors.forename: Qi
authors.initials: Q
authors.lastname: Shen
authors.forename: Ju-Mei
authors.initials: JM
authors.lastname: Zhan
authors.forename: Qun
authors.initials: Q
authors.lastname: Wang
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Zhao
mesh_terms: D000383:Agriculture; D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D018533:Biomass; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D002480:Cellulase / Q000494:pharmacology*; D004795:Enzyme Stability / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D007220:Industrial Waste; D008031:Lignin / Q000378:metabolism*; D013696:Temperature; D014908:Triticum / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D007220:Industrial Waste; C036909:lignocellulose; D008031:Lignin; D002480:Cellulase