title: Influence of monoclonal anti-Lewis b, anti-H type 1, and anti-sialyl Lewis x antibodies on binding of Helicobacter pylori to MUC1 mucin.
abstract: To assess the influence of monoclonal anti-Lewis b, anti-H type 1, and anti-sialyl Lewis x addition on interactions of sugar structures of MUC1 mucin with Helicobacter pylori. The investigations were carried out on gastric juices of 11 patients and 12 H. pylori strains. The levels of Lewis b and sialyl Lewis x antigens on MUC1 were assessed by sandwich ELISA tests. Anti-Lewis b, anti-H type 1 or anti-sialyl Lewis x monoclonal antibodies were added to MUC1 to determine whether the adhesion activities of H. pylori isolates to examined mucin would be affected. Binding of bacteria to MUC1 was assessed by ELISA test. Clear inhibitory effect of examined antibodies was revealed in 6 of 12 examined H. pylori isolates independently on babA2 status. In the rest of strains this effect was negligible. We confirmed participation of Lewis b, H type 1 and also sialyl Lewis x of MUC1 mucin in interactions with H. pylori independently on babA genopositivity. Not full inhibition and a lack of this effect in some strains suggest an existence of other mechanisms of H. pylori adherence to mucin.
authors.affiliation: Department of Medical Chemistry, Medical University of Białystok, ul Mickiewicza 2a, 15-230, Białystok 8, Poland, iwona@umb.edu.pl.
authors.forename: I
authors.initials: I
authors.lastname: Radziejewska
authors.forename: K
authors.initials: K
authors.lastname: Leszczyńska
authors.forename: M
authors.initials: M
authors.lastname: Borzym-Kluczyk
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000943:Antigens, Differentiation / Q000276:immunology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D016480:Helicobacter pylori / Q000502:physiology*; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D016256:Lewis X Antigen / Q000276:immunology*; D018396:Mucin-1 / Q000378:metabolism*; D000080506:Sialyl Lewis X Antigen
chemical_list: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; C403410:H-type-1 antigen; D007983:Lewis Blood Group Antigens; D016256:Lewis X Antigen; D018396:Mucin-1; D000080506:Sialyl Lewis X Antigen