title: In vitro evaluation of the cytotoxic and apoptogenic properties of aloe whole leaf and gel materials.
abstract: Aloe gel and whole-leaf materials have shown biological effects with potential therapeutic applications, and recently, their drug-absorption enhancement properties have been discovered. It is important to establish a safety profile for these materials before they can be used in pharmaceutical products. The aim of the study was to investigate the in vitro cytotoxicity of Aloe vera, Aloe marlothii, Aloe speciosa and Aloe ferox against human hepatocellular (HepG2), human neuroblastoma cells (SH-SY5Y) and human adenocarcinoma epithelial cells (HeLa). Flow cytometry was used to measure cell viability, apoptosis and reactive oxygen species (ROS). The aloe gel materials investigated only decreased cell viability at concentrations of >10 mg/mL and exhibited half-maximal cytotoxic concentration (CC(50)) values above 1000 mg/mL, except for A. vera gel in HepG2 cells (CC(50) = 269.3 mg/mL). A. speciosa whole-leaf material showed a significant decrease in viability of Hela cells, whereas the other whole-leaf materials did not show a similar effect. The aloe gel materials in general showed low levels of apoptosis, whereas A. vera and A. speciosa whole-leaf materials caused a dose-dependent increase of apoptosis in HeLa cells. None of the aloe materials investigated exhibited a significant increase in ROS. It can be concluded that the selected aloe materials caused only limited reduction in cell viability with limited in vitro cytotoxicity effects. Further, neither significant apoptosis effects were observed nor induction of ROS.
authors.affiliation: Centre of Excellence for Pharmaceutical Sciences, North-West University , Potchefstroom , South Africa.
authors.forename: Lissinda H
authors.initials: LH
authors.lastname: du Plessis
authors.forename: Josias H
authors.initials: JH
authors.lastname: Hamman
mesh_terms: D000504:Aloe / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage / Q000633:toxicity*; D018515:Plant Leaves; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D010936:Plant Extracts; D017382:Reactive Oxygen Species