title: Cow's milk with active immunoglobulins against Campylobacter jejuni: effects of temperature on immunoglobulin activity.
abstract: BACKGROUND
  Adult Holstein cows were injected with an antiserum against Campylobacter jejuni and immunoglobulin activities in vitro were determined in blood and milk several weeks after injection. The immunoactivity of immunoglobulins in milk was measured by an ELISA after different temperature-time treatments (60-91°C and 4-3600 s) at laboratory and pilot-plant scales. Kinetic and thermodynamic parameters were determined.
  
  
  RESULTS
  An increase in immunoglobulin activity in milk was detected several days after injection. Optical densities increased by three- to seven-fold in this period. The activity started to decay 4-5 weeks after injection. Immunoglobulins maintained most of their in vitro activity under pasteurisation conditions (72°C and 15 s) and were denatured following first-order kinetics.
  
  
  CONCLUSIONS
  The injection protocol applied allows milk with specific immunoglobulins against Campylobacter jejuni to be obtained. Traditional pasteurisation did not reduce this activity.
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Oviedo, C/Julián Clavería, 8, 33071, Oviedo, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Riera
authors.forename: Alejandro
authors.initials: A
authors.lastname: Alvarez
authors.forename: Alberto
authors.initials: A
authors.lastname: Espi
authors.forename: Miguel
authors.initials: M
authors.lastname: Prieto
authors.forename: Begoña
authors.initials: B
authors.lastname: de la Roza
authors.forename: Fernando
authors.initials: F
authors.lastname: Vicente
mesh_terms: D000818:Animals; D016123:Campylobacter jejuni / Q000276:immunology*; D002417:Cattle; D004032:Diet; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007136:Immunoglobulins / Q000097:blood / Q000378:metabolism*; D008892:Milk / Q000737:chemistry*; D061352:Pasteurization; D011489:Protein Denaturation; D013696:Temperature
chemical_list: D007136:Immunoglobulins
keywords: Campylobacter jejuni; immunoactive milk products; immunoglobulins