title: Evidence for the role of wheat eukaryotic translation initiation factor 3 subunit g (TaeIF3g) in abiotic stress tolerance.
abstract: The gene encoding eIF3g (TaeIF3g), one of the 11 subunits of eukaryotic translation initiation factor 3 (eIF3), was cloned from wheat for carrying out its functional analysis. Transgenic expression of TaeIF3g enhanced the tolerance of TaeIF3g-overexpressing parental yeast cells and Arabidopsis plants under different abiotic stress conditions. Compared to untransformed plants, TaeIF3g-overexpressing Arabidopsis thaliana plants exhibited significantly higher survival rate, soluble proteins and photosynthetic efficiency, and enhanced protection against photooxidative stress under drought conditions. This study provides first evidence that TaeIF3g imparts stress tolerance and could be a potential candidate gene for developing crop plants tolerant to abiotic stress.
authors.affiliation: Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab 143005, India; Interdisciplinary Centre for Plant Genomics and Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India.
authors.forename: Brinderjit
authors.initials: B
authors.lastname: Singh
authors.forename: Harsh
authors.initials: H
authors.lastname: Chauhan
authors.forename: Jitendra P
authors.initials: JP
authors.lastname: Khurana
authors.forename: Paramjit
authors.initials: P
authors.lastname: Khurana
authors.forename: Prabhjeet
authors.initials: P
authors.lastname: Singh
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology / Q000502:physiology; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D018556:Crops, Agricultural / Q000235:genetics; D039621:Eukaryotic Initiation Factor-3 / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D018525:Germination; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D010937:Plant Growth Regulators / Q000494:pharmacology / Q000502:physiology; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics / Q000502:physiology; D018520:Plant Shoots / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D020156:Salicylic Acid / Q000494:pharmacology; D055049:Salt Tolerance / Q000235:genetics; D013312:Stress, Physiological; D014908:Triticum / Q000235:genetics / Q000502:physiology*
chemical_list: D039621:Eukaryotic Initiation Factor-3; D010937:Plant Growth Regulators; D010940:Plant Proteins; D000040:Abscisic Acid; D020156:Salicylic Acid
keywords: ABA; Abiotic stress; Arabidopsis; BR; CS; HS; OS; SA; SS; Saccharomyces cerevisiae; Translation initiation factor 3 subunit g; Wheat; abscisic acid; brassinosteroid; cold stress; heat stress; osmotic stress; salicylic acid; salt stress