title: A conformation-switching fluorescent protein probe for detection of alpha synuclein oligomers.
abstract: We engineered the structural flexibility of an α synuclein (αS) variant, PG65, together with conformation-sensitive fluorescence to create a molecular probe for rapid, specific and quantitative detection of αS oligomers, which are the major toxic aggregate forms in Parkinson's disease.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Polytechnic Institute of NYU, 6 MetroTech Center, Brooklyn, NY 11201, USA. jkim@poly.edu.
authors.forename: Michael
authors.initials: M
authors.lastname: Hernandez
authors.forename: Yang
authors.initials: Y
authors.lastname: Hu
authors.forename: Jin Ryoun
authors.initials: JR
authors.lastname: Kim
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D015335:Molecular Probes; D010300:Parkinson Disease / Q000378:metabolism; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D051844:alpha-Synuclein / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D015335:Molecular Probes; D051844:alpha-Synuclein