title: Subchronic toxicity study of ulvan from Ulva pertusa (Chlorophyta) in Wistar rats.
abstract: Ulvan extracted from Ulva pertusa (Chlorophyta) is a group of sulfated heteropolysaccharide, for simplicity, the sulfated polysaccharide is referred to as ulvan in this paper. To our knowledge, there is no detailed report investigating the toxicity of ulvan. In this study, the subchronic (6 months) toxicity of varying levels of ulvan extracted from U. pertusa was investigated in Wistar rats after oral administration. ALT, ALB, ALP, WBC, PLT, and liver relative organ weigh of female rats showed significantly difference at 3000 mg/kg body weight per day, compared with control group. On the other hand, TG, T-CHO concentrations of female rats (6 months) were significantly decreased at 600, 1200 and 3000 mg/kg body weight per day. This result proved that ulvan had antihyperlipidemic activity. Beside, ulvan showed anticoagulant activity in this study. Overall, our findings indicated that ulvan had affected specific hematology, serum biochemistry parameters and liver, and had great differences between males and females rats.
authors.affiliation: Weifang Medical University, No. 7166 Baotong Road, Weifang 261053, PR China; Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Qi
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Liu
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.forename: Dongmei
authors.initials: D
authors.lastname: Liu
authors.forename: Liye
authors.initials: L
authors.lastname: Huang
authors.forename: Shunmei
authors.initials: S
authors.lastname: Liu
authors.forename: Quanbin
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001769:Blood / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D000960:Hypolipidemic Agents / Q000494:pharmacology / Q000633:toxicity; D008297:Male; D009026:Mortality; D009929:Organ Size / Q000187:drug effects; D011134:Polysaccharides / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D059550:Toxicity Tests, Subchronic / Q000379:methods*; D044445:Ulva / Q000737:chemistry
chemical_list: D000960:Hypolipidemic Agents; D011134:Polysaccharides; C571831:ulvan
keywords: ALP; ALT; APPT; AST; Alb; BUN; CK; Cl; Crea; HDL; K; LDL; Na; PT; Part prothrombin time; Prothrombin Time; Rats; Subchronic toxicity; T-CHO; T. Bil; TG; TP; U; Ulva pertusa; Ulvan; alanine aminotransferase; albumin; asparatate aminotransferase; aspartate aminotransferase; chloride; creatine kinase; creatinine; high density lipoprotein; low density lipoprotein; potassium; sodium; total bilirubin; total blood urea nitrogrn; total cholesterol; total glyceride; total protein; ulvan