title: Evaluation of serum levels of multiple cytokines and adhesion molecules in patients with newly diagnosed acute lymphoblastic leukemia using biochip array technology.
abstract: OBJECTIVE
  Evaluation of serum levels of 17 cytokines and 5 adhesion molecules in patients with acute lymphoblastic leukemia (ALL) and in healthy subjects using biochip array technology. This approach allows multi-analytical determination from a single sample.
  
  
  METHODS
  A total of 15 ALL patients and 15 healthy subjects (blood donors) were studied. Serum samples were analyzed by biochip based immunoassays on the Evidence Investigator analyzer. T-tests were used for statistical analysis.
  
  
  RESULTS
  Comparing cytokine and adhesion molecule levels in ALL patients and in healthy subject, we found significant increase in serum VCAM-1 (p < 0.000001), ICAM-1 (p < 0.0001), L-selectin (p < 0.0001), IL-8 (p < 0.001), MCP-1 (p < 0.01), and significant decrease (p < 0.01) in serum IL-3 and IL-4.
  
  
  CONCLUSIONS
  Our results indicate that serum levels of specific cytokines and adhesion molecules (VCAM-1, ICAM-1, L-selectin, IL-8, IL-3, IL-4, MCP-1) are significantly altered in patients with newly diagnosed ALL, reflecting acti-vity of the disease. Further investigation is needed to establish if these alterations could be used as a prognostic indicator for ALL.
authors.affiliation: Department of Internal Medicine, University of Defense, Faculty of MilitaryHealth Sciences in Hradec Kralove, Czech Republic.
authors.forename: J M
authors.initials: JM
authors.lastname: Horacek
authors.forename: T
authors.initials: T
authors.lastname: Kupsa
authors.forename: M
authors.initials: M
authors.lastname: Vasatova
authors.forename: L
authors.initials: L
authors.lastname: Jebavy
authors.forename: P
authors.initials: P
authors.lastname: Zak
mesh_terms: D000328:Adult; D015815:Cell Adhesion Molecules / Q000097:blood*; D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood*; D040081:Protein Array Analysis / Q000379:methods*; D055815:Young Adult
chemical_list: D015815:Cell Adhesion Molecules; D016207:Cytokines