title: Multidimensional separation prior to mass spectrometry: getting closer to the bottom of the iceberg.
abstract: While prefractionation has previously been shown to improve results in MS analysis, a novel combination provides an additional dimension of separation: protein fractionation by SDS-PAGE followed by IEF of tryptic peptides before separation by RP-LC [Atanassov and Urlaub, Proteomics 2013, 13, 2947-2955]. This three-step separation procedure prior to MS/MS substantially increases proteome coverage and represents a further step toward a more comprehensive analysis of complex proteomes.
authors.affiliation: King's British Heart Foundation Centre, King's College London, London, UK.
authors.forename: Manuel
authors.initials: M
authors.lastname: Mayr
authors.forename: Thierry
authors.initials: T
authors.lastname: Rabilloud
mesh_terms: D005591:Chemical Fractionation; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007525:Isoelectric Focusing; D013058:Mass Spectrometry / Q000379:methods*; D011506:Proteins / Q000302:isolation & purification*; D040901:Proteomics
chemical_list: D011506:Proteins
keywords: Electrophoresis; Isoelectric focusing; Liquid-chromatography; Prefractionation