title: Effect of black grape juice against heart damage from acute gamma TBI in rats.
abstract: The aim of this study was to evaluate the potential positive effect of black grape juice (BGJ) on lipid peroxidation considering Total Body Irradiation (TBI) in Wistar rats. As a potential feasible means of evaluation in situ, blood serum lactate dehydrogenase (LDH) levels were evaluated as a marker for heart damage from acute radiation syndrome (ARS). Twenty rats were divided into four groups, two of them being irradiated by gamma-rays from a Co-60 source. Animals were treated by gavage with 2 mL per day of BGJ or placebo for one week before and 4 days after 6 Gy whole body gamma-irradiation, when they were euthanasiated. LDH on serum and lipid peroxidation on heart tissue were evaluated. High concentration of metabolites from lipid peroxidation in heart, and high LDH level on serum were found only in gamma-irradiated group given placebo, mainly at the first 24 h after radiation. Phytochemical analysis of BGJ was performed by determining total phenolics, flavonoids, and tannins followed by a high-performance liquid chromatography (HPLC/DAD) analysis, which showed resveratrol as the major constituent. Results suggest that BGJ is a good protective candidate compound against heart damage from ARS and its effects suggest its use as a radiomodifier.
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil. lfgbauer@smail.ufsm.br.
authors.forename: Robson Borba
authors.initials: RB
authors.lastname: de Freitas
authors.forename: Aline Augusti
authors.initials: AA
authors.lastname: Boligon
authors.forename: Bruno Tomazele
authors.initials: BT
authors.lastname: Rovani
authors.forename: Mariana
authors.initials: M
authors.lastname: Piana
authors.forename: Thiele Faccim
authors.initials: TF
authors.lastname: de Brum
authors.forename: Roberta
authors.initials: R
authors.lastname: da Silva Jesus
authors.forename: Fagner Chagas
authors.initials: FC
authors.lastname: Rother
authors.forename: Nelson Mendes
authors.initials: NM
authors.lastname: Alves
authors.forename: João Batista
authors.initials: JB
authors.lastname: Teixeira da Rocha
authors.forename: Margareth Linde
authors.initials: ML
authors.lastname: Athayde
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Barrio
authors.forename: Edson Ramos
authors.initials: ER
authors.lastname: de Andrade
authors.forename: Liliane
authors.initials: L
authors.lastname: de Freitas Bauerman
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D005638:Fruit / Q000737:chemistry; D005720:Gamma Rays; D006321:Heart / Q000187:drug effects* / Q000528:radiation effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D011833:Radiation Injuries, Experimental / Q000188:drug therapy* / Q000378:metabolism; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D027843:Vitis / Q000737:chemistry*; D014916:Whole-Body Irradiation
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase