title: Imaging InlC secretion to investigate cellular infection by the bacterial pathogen Listeria monocytogenes.
abstract: Bacterial intracellular pathogens can be conceived as molecular tools to dissect cellular signaling cascades due to their capacity to exquisitely manipulate and subvert cell functions which are required for the infection of host target tissues. Among these bacterial pathogens, Listeria monocytogenes is a Gram positive microorganism that has been used as a paradigm for intracellular parasitism in the characterization of cellular immune responses, and which has played instrumental roles in the discovery of molecular pathways controlling cytoskeletal and membrane trafficking dynamics. In this article, we describe a robust microscopical assay for the detection of late cellular infection stages of L. monocytogenes based on the fluorescent labeling of InlC, a secreted bacterial protein which accumulates in the cytoplasm of infected cells; this assay can be coupled to automated high-throughput small interfering RNA screens in order to characterize cellular signaling pathways involved in the up- or down-regulation of infection.
authors.affiliation: Unité des Interactions Bactéries Cellules, Pasteur Institute.
authors.forename: Andreas
authors.initials: A
authors.lastname: Kühbacher
authors.forename: Edith
authors.initials: E
authors.lastname: Gouin
authors.forename: Jason
authors.initials: J
authors.lastname: Mercer
authors.forename: Mario
authors.initials: M
authors.lastname: Emmenlauer
authors.forename: Christoph
authors.initials: C
authors.lastname: Dehio
authors.forename: Pascale
authors.initials: P
authors.lastname: Cossart
authors.forename: Javier
authors.initials: J
authors.lastname: Pizarro-Cerdá
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008089:Listeria monocytogenes / Q000737:chemistry / Q000235:genetics / Q000472:pathogenicity*; D008088:Listeriosis / Q000382:microbiology; D008856:Microscopy, Fluorescence / Q000379:methods; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection
chemical_list: D001426:Bacterial Proteins; D034741:RNA, Small Interfering; C103178:inlC protein, Listeria monocytogenes