title: Relationship between reactive oxygen species production in human semen and sperm DNA damage assessed by Sperm Chromatin Structure Assay.
abstract: OBJECTIVE
  The aim of this prospective study was to find possible relationship between ROS production measured by chemiluminescence and flow cytometry in human semen and sperm DNA damage estimated by Sperm Chromatin Structure Assay.
  
  
  METHODS
  Study included 39 men from infertile couples and 23 fertile volunteers who served as a control group. Aliquot of neat semen was used for ROS detection by chemiluminescence. Aliquot of sperm suspension in phosphate buffered saline was used for the detection of ROS by flow cytometry. Another aliquot of sperm suspension was used for SCSA to measure DNA fragmentation index and High DNA stainability.
  
  
  RESULTS
  DNA fragmentation index correlated negatively with sperm morphology and motility. High DNA stainability correlated positively with ROS production and negatively with sperm morphology and concentration. Although there were similar trends of rising DNA fragmentation index and ROS production among the three groups of men, the relationship did not reach statistical significance.
  
  
  CONCLUSIONS
  Higher values of DNA fragmentation index and high DNA stainability may also reflect developmental and/or environmental problems and not only oxidative stress.
authors.affiliation: Department of Biology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Novotny
authors.forename: Nabil
authors.initials: N
authors.lastname: Aziz
authors.forename: Roman
authors.initials: R
authors.lastname: Rybar
authors.forename: Jana
authors.initials: J
authors.lastname: Brezinova
authors.forename: Vera
authors.initials: V
authors.lastname: Kopecka
authors.forename: Radka
authors.initials: R
authors.lastname: Filipcikova
authors.forename: Marie
authors.initials: M
authors.lastname: Reruchova
authors.forename: Ivana
authors.initials: I
authors.lastname: Oborna
mesh_terms: D000328:Adult; D002843:Chromatin; D004249:DNA Damage; D005821:Genetic Techniques; D006801:Humans; D008297:Male; D011446:Prospective Studies; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012661:Semen / Q000378:metabolism*; D013094:Spermatozoa
chemical_list: D002843:Chromatin; D017382:Reactive Oxygen Species