title: Physiological and proteomic analyses of drought stress response in Holm oak provenances.
abstract: Responses to drought stress by water withholding have been studied in 1 year old Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) seedlings from seven provenances from Andalusia (southern Spain). Several physiological parameters, including predawn xylem water potentials and relative water content in soil, roots, and leaves as well as maximum quantum efficiency and yield of PSII were evaluated for 28 days in both irrigated and nonirrigated seedlings. The leaf proteome map of the two provenances that show the extreme responses (Seville, GSE, is the most susceptible, while Almerı́a, SSA, is the least susceptible) was obtained. Statistically significant variable spots among provenances and treatments were subjected to MALDI-TOF/TOF-MS/MS analysis for protein identification. In response to drought stress, ~12.4% of the reproducible spots varied significantly depending on the treatment and the population. These variable proteins were mainly chloroplastic and belonged to the metabolism and defense/stress functional categories. The 2-DE protein profile of nonirrigated seedlings was similar in both provenances. Physiological and proteomics data were generally in good agreement. The general trend was a decrease in protein abundance upon water withholding in both provenances, mainly in those involved in ATP synthesis and photosynthesis. This decrease, moreover, was most marked in the most susceptible population compared with the less susceptible one.
authors.affiliation: Department of Chemistry-Biology, Biomedical Sciences Institute, Autonomous University of Ciudad Juárez , Anillo Envolvente del Pronaf y Estocolmo s/n, 32310 Ciudad Juárez, Chihuahua, México.
authors.forename: José
authors.initials: J
authors.lastname: Valero-Galván
authors.forename: Raquel
authors.initials: R
authors.lastname: González-Fernández
authors.forename: Rafael Maria
authors.initials: RM
authors.lastname: Navarro-Cerrillo
authors.forename: Eustaquio
authors.initials: E
authors.lastname: Gil-Pelegrín
authors.forename: Jesús V
authors.initials: JV
authors.lastname: Jorrín-Novo
mesh_terms: D000704:Analysis of Variance; D055864:Droughts; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant / Q000235:genetics* / Q000502:physiology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D029963:Quercus / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism; D013030:Spain; D013312:Stress, Physiological / Q000235:genetics* / Q000502:physiology; D053719:Tandem Mass Spectrometry
chemical_list: D010940:Plant Proteins