title: Characterization and application of aminoamide-oxidizing enzyme from Aspergillus carbonarius AIU 205.
abstract: We isolated Aspergillus carbonarius AIU 205 as a new producer of an enzyme catalyzing oxidative deamination of 4-aminobutanamide (4-ABAD) to 4-oxobutanamide with the subsequent release of ammonia and hydrogen peroxide. Since the strain produced three enzymes with different Km values for 4-ABAD, the enzyme with lowest Km value (0.31 mM) was purified and revealed certain remarkable properties. The enzyme also oxidized aliphatic monoamines, aromatic amines and aliphatic aminoalcohols, but did not oxidize l-amino acids and aliphatic diamines. The Vmax/Km values for aliphatic monoamines were higher than that for 4-ABAD, and the enzyme activity was strongly inhibited by inhibitors of copper-containing amine oxidases. Thus, it was concluded that the enzyme might belong to a group of copper-containing amine oxidase. The 4-ABAD oxidase activity of this enzyme was optimum at pH 7.0, and the enzyme activity at pH 6.0 was 65% of that at pH 7.0. The enzyme was useful for increasing the sensitivity of l-lysine assay using l-amino acid oxidase/monooxygenase from Pseudomonas sp. AIU 813.
authors.affiliation: Department of Biological Chemistry and Food Science, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan.
authors.forename: Asami
authors.initials: A
authors.lastname: Sugawara
authors.affiliation: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Matsui
authors.affiliation: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan.
authors.forename: Hidenobu
authors.initials: H
authors.lastname: Komeda
authors.affiliation: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan.
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Asano
authors.affiliation: Department of Biological Chemistry and Food Science, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan. Electronic address: kiso@iwate-u.ac.jp.
authors.forename: Kimiyasu
authors.initials: K
authors.lastname: Isobe
mesh_terms: D000577:Amides / Q000378:metabolism*; D006631:Amine Oxidase (Copper-Containing) / Q000302:isolation & purification / Q000378:metabolism*; D001230:Aspergillus / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D050830:L-Amino Acid Oxidase / Q000302:isolation & purification / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000201:enzymology; D013379:Substrate Specificity; D013696:Temperature
chemical_list: C000588490:4-aminobutanamide; D000577:Amides; D050830:L-Amino Acid Oxidase; D006631:Amine Oxidase (Copper-Containing); D008239:Lysine
keywords: 4-Aminobutanamide; Amine oxidase; Aminoamide; Aspergillus carbonarius; l-Amino acid oxidase/oxygenase; l-Lysine; l-Ornithine