title: Cloning and expression of selenoprotein W from pearl mussels Cristaria plicata.
abstract: Selenoprotein W (SelW) is a selenocysteine containing protein with redox activity involved in the antioxidant response. In this study, a selenoprotein W was cloned from pearl mussel Cristaria plicata (designated as CpSelW), and the expression patterns were characterized in tissues after Aeromonas hydrophila challenged. The full-length cDNA of cpSelW was of 858bp, containing a 5' untranslated region (UTR) of 145bp, a 3' UTR of 455bp with a poly (A) tail, and an open reading frame (ORF) of 258bp encoding a polypeptide of 85 amino acids with the predicted molecular mass of 9.277kDa, which shared 61% identity with SelW from Gallus gallus. A tertiary structure model generated for the CpSelW displayed a β-α-β-β-β-α secondary structure pattern, which was similar to mouse SelW protein 3D structure. The mRNA of CpSelW was constitutively expressed in tested tissues of healthy mussel, including mantle, gill, hemocytes, muscle, and hepatopancreas, and it was highly expressed in hepatopancreas. After mussels were stimulated by A. hydrophila, the mRNA expression of CpSelW in hemocytes at 6, 12 and 24h, in gill at 12h and in hepatopancreas at 24h was significantly down-regulated.
authors.affiliation: School of Life Sciences and Food Engineering, Institute of Life Science, Nanchang University, Nanchang 330031, China.
authors.forename: Bao-Qing
authors.initials: BQ
authors.lastname: Hu
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Chun-Gen
authors.initials: CG
authors.lastname: Wen
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Li
authors.forename: Xiang-Ping
authors.initials: XP
authors.lastname: Hu
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Hu
authors.forename: Zhi-Ying
authors.initials: ZY
authors.lastname: Tao
mesh_terms: D016980:Aeromonas hydrophila / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000235:genetics* / Q000382:microbiology; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051151:Selenoprotein W / Q000737:chemistry / Q000235:genetics*; D017385:Sequence Homology
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D051151:Selenoprotein W
keywords: Cristaria plicata; Gene cloning; Selenoprotein W (SelW); mRNA expression