title: Optical neuritis induced by different concentrations of myelin oligodendrocyte glycoprotein presents different profiles of the inflammatory process.
abstract: Optical neuritis (ON) is characterized by inflammation of the optic nerve, and is one of the first clinical signs of multiple sclerosis (MS). Experimental autoimmune encephalomyelitis (EAE) is the animal model used to study MS and ON. The present study evaluated the induction, development and progression of ON using an EAE model induced by 100 μg or 300 μg of MOG35-55. An EAE model was induced in C57BL/6 mice by tail base injection of 100 μg or 300 μg of MOG35-55 in complete Freund's adjuvant, supplemented with Mycobacterium tuberculosis. On the day of injection and 48 h later, animals received intraperitoneally 300 ng of pertussis toxin. On days 7, 10, 14, 21 and 58 the optic nerve was dissected for histological analysis, production of CCL5 and immunohistochemical detection of CD4 and CD8. The histological changes observed in the optic nerves consisted of inflammatory cell infiltrates showing varying degrees of ON in the two groups. The onset of ON in the 300 μg of MOG35-55 group was coincident with higher production of CCL5, on day 10 after induction. However, the 100 μg MOG35-55 group showed more intense inflammatory infiltrate on day 14 after induction, with higher amounts of CD4 and CD8, reaching an excessive demyelination process on days 21 and 58 after induction. The results suggest that two different concentrations of MOG35-55 lead to different forms of evolution of optic neuritis.
authors.affiliation: Department of Ophthalmology, University Hospital of the Federal University of Juiz de Fora , Juiz de Fora , Brazil .
authors.forename: Rubens Murilo Gibaile
authors.initials: RM
authors.lastname: Soares
authors.forename: Alyria Teixeira
authors.initials: AT
authors.lastname: Dias
authors.forename: Sandra Bertelli Ribeiro
authors.initials: SB
authors.lastname: De Castro
authors.forename: Caio Cesar Souza
authors.initials: CC
authors.lastname: Alves
authors.forename: Marcilene Gomes
authors.initials: MG
authors.lastname: Evangelista
authors.forename: Luan Cristian
authors.initials: LC
authors.lastname: Da Silva
authors.forename: Rogério Estevam
authors.initials: RE
authors.lastname: Farias
authors.forename: Maria Christina Marques Nogueira
authors.initials: MC
authors.lastname: Castanon
authors.forename: Maria Aparecida
authors.initials: MA
authors.lastname: Juliano
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D015704:CD4 Antigens / Q000096:biosynthesis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D016827:CD8 Antigens / Q000096:biosynthesis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D018946:Chemokine CCL5 / Q000096:biosynthesis; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology; D005260:Female; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D018836:Inflammation Mediators / Q000008:administration & dosage* / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009103:Multiple Sclerosis / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000008:administration & dosage* / Q000502:physiology; D009902:Optic Neuritis / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D010446:Peptide Fragments / Q000008:administration & dosage / Q000502:physiology
chemical_list: D015704:CD4 Antigens; D016827:CD8 Antigens; C503460:Ccl5 protein, mouse; D018946:Chemokine CCL5; D018836:Inflammation Mediators; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; C445081:myelin oligodendrocyte glycoprotein (35-55)