title: The function of Msx1 gene in promoting meiosis of dairy goat male germline stem cells (mGSCs).
abstract: During sequential stages of meiosis, numerous cytoplasmic and nuclear events take place in which many germline and non-germline genes involved. It is demonstrated that the germline gene Stra8 and synaptonemal complex protein 3 (Scp3) play an important role in the meiosis. Recently, studies showed Msx1, a DNA-binding protein taking part in the skeletal development, also having a functional attractive factor to Stra8 and Scp3 in the meiosis. In this study, we cloned the gene Msx1 then transfected the Msx1 constructed recombination plasmid, pMsx1-Ires2-AcGFP, into the dairy goat germline stem cells (male germline stem cells) and analysed the effects of Msx1 on the expression of Stra8 and Scp3. The results showed that Msx1 could enhance the expression of Stra8 and Scp3 and promote the meiosis in goat testicular cells. Bmp4 activated the expression of Msx1 and Stra8. This study suggests that Msx1 plays an important role in spermatogenesis and meiosis.
authors.affiliation: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering and Technology, Key Lab for Animal Biotechnology of Agriculture Ministry of China, Northwest A&F University, Yangling, China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Mu
authors.forename: Jiang
authors.initials: J
authors.lastname: Wu
authors.forename: Haijing
authors.initials: H
authors.lastname: Zhu
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Furong
authors.initials: F
authors.lastname: Tang
authors.forename: Xi
authors.initials: X
authors.lastname: Yao
authors.forename: Churong
authors.initials: C
authors.lastname: Yang
authors.forename: Sha
authors.initials: S
authors.lastname: Peng
authors.forename: Guangpeng
authors.initials: G
authors.lastname: Li
authors.forename: Jinlian
authors.initials: J
authors.lastname: Hua
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism*; D000818:Animals; D005854:Germ Cells / Q000166:cytology* / Q000378:metabolism*; D006041:Goats; D051957:MSX1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008540:Meiosis / Q000235:genetics; D013737:Testis / Q000166:cytology*
chemical_list: D051957:MSX1 Transcription Factor
keywords: Msx1 Stra8 Scp3 Meiosis mGSCs Bmp4