title: [Effects of platelet-rich fibrin on canine dental pulp cells in vitro].
abstract: OBJECTIVE
  To investigate the effect of platelet-rich fibrin (PRF) on the proliferation and chemotaxis capacity of autogenous canine dental pulp cells (cDPCs), and to evaluate the use of PRF as a pulp capping material in vital pulp therapy.
  
  
  METHODS
  cDPCs were isolated and cultured from permanent anterior teeth of Beagle dogs by enzymatic methods. PRF was attained by Choukroun's protocols and the exudates of PRF were collected at the time point of the 7th day. Cell counting kit-8 (CCK-8) was applied to analyze cell proliferation. The medium of the control group was minimum essential medium alpha medium (α-MEM) containing 2% (volume fraction) fetal bovine serum (FBS). The experiment group was the exudates of PRF containing 2% FBS, and was divided into 5 groups (20%, 40%, 60%, 80%, 100%) by the volume fraction of the exudates of PRF. The 5 groups were named as PRF1, PRF2, PRF3, PRF4 and PRF5 respectively. Transwell model was used to evaluate cell chemotaxis capacity. The exudates of PRF which was most effectively in promoting cDPCs proliferation was added in the lower chamber of the experimental group; The positive control group was α-MEM containing 30% FBS and the negative control group was fresh α-MEM; The upper chamber of each group was added with 1 × 105 cells.
  
  
  RESULTS
  The optical density of group PRF2 (1.45 ± 0.06) was significantly higher than that of the control group(1.21 ± 0.11, P<0.001). The optical density of groups PRF1, PRF3, PRF4, and PRF5 were 1.20 ± 0.02, 1.28 ± 0.04, 1.19 ± 0.02, 1.22 ± 0.02, respectively, and there was no significant difference between these groups and the control group (PRF1: P=0.902; PRF3: P=0.084; PRF4: P=0.726; PRF5: P=0.779). Therefore, the volume fraction which was most effective in promoting cell proliferation was 40%. At these concentrations the number of migrated cells was higher in PRF group(55.89 ± 18.42) than in the negative group(6.52 ± 1.97, P<0.001), but there was no significant difference between PRF group and the positive group(59.25 ± 29.17, P=0.970).
  
  
  CONCLUSIONS
  PRF was a biocompatible material with cDPCs. Appropriate concentration of the exudates of PRF accelerated the proliferation and migration of cDPCs, which contributes to pulp repair in vital pulp therapy.
authors.affiliation: Department of Pediatric Dentistry,Peking Unversity School and Hospital of Stomatology,Beijing 100081,China.
authors.forename: Pan-Pan
authors.initials: PP
authors.lastname: Yang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhan
authors.forename: Sheng-Lin
authors.initials: SL
authors.lastname: Li
authors.forename: He
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D001792:Blood Platelets; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects*; D003782:Dental Pulp / Q000166:cytology*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D005337:Fibrin / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male
chemical_list: D005337:Fibrin