title: Bleomycin inhibits adipogenesis and accelerates fibrosis in the subcutaneous adipose layer through TGF-β1.
abstract: Systemic sclerosis [scleroderma (SSc)]-associated skin fibrosis is characterized by increased fibrosis in the dermis and a reduction in the thickness of the subcutaneous adipose tissue layer. Although many studies have examined fibrosis in SSc, only a few studies have focused on the associated reduction in the thickness of the subcutaneous adipose tissue layer. In this study, we investigated the effects of SSc-induced fibrosis on adipose tissue. We found that bleomycin suppresses adipogenesis in adipose-derived stem cells (ASCs) and stimulates ASCs to express transforming growth factor β1 (TGF-β1), which suppresses adipogenesis and promotes fibrosis. Furthermore, we found that adipocyte-conditioned medium suppressed collagen synthesis by fibroblasts in fibrosis-like conditions. We concluded that in the skin affected by bleomycin-induced fibrosis, increased TGF-β1 expression suppresses adipogenesis and promotes adipocyte fibrosis. It was also suggested that adipocytes have an inhibitory effect on the progression of fibrosis.
authors.affiliation: Research Laboratories, Nippon Menard Cosmetic Co., Ltd., Nagoya, Japan.
authors.forename: Shiro
authors.initials: S
authors.lastname: Ohgo
authors.forename: Seiji
authors.initials: S
authors.lastname: Hasegawa
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Hasebe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mizutani
authors.forename: Satoru
authors.initials: S
authors.lastname: Nakata
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Akamatsu
mesh_terms: D017667:Adipocytes / Q000166:cytology; D050156:Adipogenesis / Q000187:drug effects*; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000737:chemistry; D001761:Bleomycin / Q000737:chemistry*; D002454:Cell Differentiation; D003094:Collagen / Q000096:biosynthesis; D017077:Culture Media, Conditioned / Q000737:chemistry; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005355:Fibrosis / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D051379:Mice; D012595:Scleroderma, Systemic / Q000473:pathology; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D000903:Antibiotics, Antineoplastic; D017077:Culture Media, Conditioned; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D001761:Bleomycin; D003094:Collagen
keywords: TGF-β1; adipogenesis; bleomycin; skin fibrosis; systemic sclerosis