title: Comparison of the buccal mucosal bleeding time in dogs using 3 different-sized lancet devices.
abstract: BACKGROUND
  The buccal mucosal bleeding time (BMBT) evaluates primary hemostasis in vivo. Three different-sized lancet devices designed for people, Adult (A), Junior (J), and Newborn (N), can be used to perform the BMBT in dogs.
  
  
  OBJECTIVE
  The aim was to compare BMBT using 3 different-sized human lancet devices in dogs with varying platelet counts and hematocrits.
  
  
  METHODS
  The BMBT was measured in 46 client-owned dogs (2 healthy, 44 suffering from various disorders) with varying platelet (Plt) counts and hematocrits, using the 3 devices successively in each dog, in a randomly determined order, over a 10- to 30-minute period. Statistical analysis (ANOVA, Mann-Whitney U-test) was performed using commercial software.
  
  
  RESULTS
  BMBTs were significantly different between devices (P < .00001), and shorter with devices N and J compared with device A (P < .01). The BMBT was prolonged (> 210 s) in 10 dogs with device A and in 7 dogs each with devices J and N, respectively. Sixteen dogs had a Plt count < 200 × 10(9) /L (Reference interval 200-500 × 10(9) /L). Nine of these dogs had prolonged BMBT with device A, and 6 dogs with device J and device N, respectively. BMBT was longer in thrombocytopenic dogs with devices A and J (P < .016). Anemia without thrombocytopenia did not affect BMBT with any device.
  
  
  CONCLUSIONS
  The BMBT is influenced by the size of the used device, with A resulting in the longest BMBT. Therefore, the type of device used to obtain the BMBT has to be specified for standardized results.
authors.affiliation: Internal Medicine, Department of Clinical Sciences, Université de Toulouse, INPT, ENVT, Toulouse, France.
authors.forename: Marcel
authors.initials: M
authors.lastname: Aumann
authors.forename: Valentina
authors.initials: V
authors.lastname: Rossi
authors.forename: Kevin
authors.initials: K
authors.lastname: Le Boedec
authors.forename: Armelle
authors.initials: A
authors.lastname: Diquélou
mesh_terms: D000818:Animals; D001760:Bleeding Time / Q000662:veterinary; D001800:Blood Specimen Collection / Q000009:adverse effects / Q000295:instrumentation*; D004285:Dogs; D005260:Female; D006487:Hemostasis; D008297:Male; D009061:Mouth Mucosa / Q000502:physiology*; D010976:Platelet Count / Q000662:veterinary; D013921:Thrombocytopenia / Q000662:veterinary*
keywords: Dogs; hemorrhage; mucosa; platelets; primary hemostasis