title: Effect of 5- AZn-2 '-deoxycytidine on proliferation of human lung adenocarcinoma cell line A549 in vitro.
abstract: OBJECTIVE
  To explore effect of 5-AZn-2 '-deoxycytidine on proliferation of human lung adenocarcinoma cell line A549 in vitro.
  
  
  METHODS
  Superoxide dismutase (SOD) activity was measured by hydroxylamine colorimetric method. Inhibition effect of 5-AZn-2' deoxycytidylic acid at different concentration and different time on growth of A549 cell was determined by MTT assay. Methylene dioxyamphetamine (MDA) was measured by thiobarbituric acid colorimetric method. Effect of 5-AZn-2' deoxycytidylic acid on apoptosis of A549 cell was determined by Hoechst 33258 dyeing detection.
  
  
  RESULTS
  5-AZn-2' deoxycytidylic acid had significant inhibition effect on proliferation of A549 cells in vitro, and the inhibition was notably dependent on time and dosage during 48-72 h; SOD level was significantly lower than those of control group (P<0.05, P<0.01), MDA level was significantly higher than those in the control group (P<0.05, P<0.01). A549 cells began to be in apoptosis after using 5-AZn-2'deoxycytidylic acid.
  
  
  CONCLUSIONS
  5- AZn-2' deoxycytidylic acid has significant inhibition effect on growth of A549 cell, and can lead the change of lipid peroxidation. It indicates that the mechanism has relationship with A549 cell cycle tissue and induction factor of apoptosis.
authors.affiliation: Department of Intensive Care Unit, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Hua-Qiong
authors.initials: HQ
authors.lastname: Huang
authors.forename: Gen-Sheng
authors.initials: GS
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Cui
mesh_terms: D000230:Adenocarcinoma; D000077192:Adenocarcinoma of Lung; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D048430:Cell Shape / Q000187:drug effects; D000077209:Decitabine; D006801:Humans; D008175:Lung Neoplasms; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism
chemical_list: D000964:Antimetabolites, Antineoplastic; D008315:Malondialdehyde; D000077209:Decitabine; D013482:Superoxide Dismutase; D001374:Azacitidine
keywords: A549 cell; Apoptosis; DAC; Lipid peroxidation