title: Anti-listeria activity of poly(lactic acid)/sawdust particle biocomposite film impregnated with pediocin PA-1/AcH and its use in raw sliced pork.
abstract: A novel poly(lactic acid) (PLA)/sawdust particle (SP) biocomposite film with anti-listeria activity was developed by incorporation of pediocin PA-1/AcH (Ped) using diffusion coating method. Sawdust particle played an important role in embedding pediocin into the hydrophobic PLA film. The anti-listeria activity of the PLA/SP biocomposite film incorporated with Ped (PLA/SP+Ped) was detected, while no activity against the tested pathogen was observed for the control PLA films (without SP and/or Ped). Dry-heat treatment of film before coating with Ped resulted in the highest Ped adsorption (11.63 ± 3.07 μg protein/cm(2)) and the highest anti-listeria activity. A model study of PLA/SP+Ped as a food-contact antimicrobial packaging on raw sliced pork suggests a potential inhibition of Listeria monocytogenes (99% of total listerial population) on raw sliced pork during the chilled storage. This study supports the feasibility of using PLA/SP+Ped film to reduce the initial load of L. monocytogenes on the surface of raw pork.
authors.affiliation: Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, 15 Karnjanavanich Road, Songkhla 90110, Thailand.
authors.forename: Weerapong
authors.initials: W
authors.lastname: Woraprayote
authors.forename: Yutthana
authors.initials: Y
authors.lastname: Kingcha
authors.forename: Pannawit
authors.initials: P
authors.lastname: Amonphanpokin
authors.forename: Jittiporn
authors.initials: J
authors.lastname: Kruenate
authors.forename: Takeshi
authors.initials: T
authors.lastname: Zendo
authors.forename: Kenji
authors.initials: K
authors.lastname: Sonomoto
authors.forename: Soottawat
authors.initials: S
authors.lastname: Benjakul
authors.forename: Wonnop
authors.initials: W
authors.lastname: Visessanguan
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D001430:Bacteriocins / Q000494:pharmacology*; D005506:Food Contamination / Q000517:prevention & control; D018857:Food Packaging; D019344:Lactic Acid / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects*; D008460:Meat / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D000070017:Pediocins; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology*; D012995:Solubility; D013552:Swine; D013718:Tensile Strength
chemical_list: D000890:Anti-Infective Agents; D001430:Bacteriocins; D000070017:Pediocins; D011091:Polyesters; D011108:Polymers; C074288:pediocin PA-1; D019344:Lactic Acid; C033616:poly(lactide)
keywords: Anti-listeria; Biocomposite film; Packaging; Pediocin; Poly(lactic acid); Sawdust particle