title: The therapeutic effects of anti-oxidant and anti-inflammatory drug quercetin on aspiration-induced lung injury in rats.
abstract: Aspiration pneumonitis refers to acute chemical lung injury caused by aspiration of sterile gastric contents. The aim of this study was to evaluate the role of quercetin (QC) in acid aspiration-induced lung injury in rats. Twenty-eight female Sprague-Dawley rats were used and divided into the following groups (n = 7): sham (aspirated normal saline, S), hydrochloric acid (aspirated HCl), S plus treatment with QC (S + QC), and HCl plus treatment with QC (HCl + QC). After aspiration, the treatment groups received QC 60 mg/kg/day intraperitoneally once a day for 7 days. As a result of acid aspiration, an increase was observed in the levels of serum clara cell protein-16 (CC-16) and advanced oxidation protein products, whereas there was a decrease in serum thiobarbituric acid-reactive substances, superoxide dismutase (SOD), and catalase levels. There was a significant decrease in peribronchial inflammatory cell infiltration, alveolar septal infiltration, alveolar edema, and alveolar exudate scores, except in the alveolar histiocytes in the HCl + QC group. The expression of nitric oxide synthase, which increased after aspiration in the HCl group, showed a statistically significant decrease after the QC treatment. After the treatment with QC, an increase in the serum SOD level was observed, whereas a significant decrease was determined in the serum CC-16 level relative to that of the aspiration group (HCl). The antioxidant QC is effective in the treatment of lung injury following acid aspiration and can be used as a serum CC-16 biomarker in predicting the severity of oxidative lung injury.
authors.affiliation: Department of Pedodontia, Faculty of Dentistry, Ondokuz Mayis University, Samsun, Turkey, drziyilmaz@gmail.com.
authors.forename: Mehmet Ziya
authors.initials: MZ
authors.lastname: Yilmaz
authors.forename: Aygul
authors.initials: A
authors.lastname: Guzel
authors.forename: Aysun Caglar
authors.initials: AC
authors.lastname: Torun
authors.forename: Ali
authors.initials: A
authors.lastname: Okuyucu
authors.forename: Osman
authors.initials: O
authors.lastname: Salis
authors.forename: Rifat
authors.initials: R
authors.lastname: Karli
authors.forename: Ayhan
authors.initials: A
authors.lastname: Gacar
authors.forename: Tolga
authors.initials: T
authors.lastname: Guvenc
authors.forename: Sule
authors.initials: S
authors.lastname: Paksu
authors.forename: Volkan
authors.initials: V
authors.lastname: Urey
authors.forename: Naci
authors.initials: N
authors.lastname: Murat
authors.forename: Hasan
authors.initials: H
authors.lastname: Alacam
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D002374:Catalase / Q000097:blood; D005260:Female; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D011015:Pneumonia, Aspiration / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D011794:Quercetin / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D011794:Quercetin; D002374:Catalase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D013482:Superoxide Dismutase