title: Mobuck virus genome sequence and phylogenetic analysis: identification of a novel Orbivirus isolated from a white-tailed deer in Missouri, USA.
abstract: The genus Orbivirus includes a diverse group of segmented dsRNA viruses that are transmitted via arthropods, have a global distribution and affect a wide range of hosts. A novel orbivirus was co-isolated with epizootic haemorrhagic disease virus (EHDV) from a white-tailed deer (Odocoileus virginianus) exhibiting clinical signs characteristic of EHDV. Using antiserum generated against EHDV, a pure isolate of the novel non-cytopathic orbivirus was obtained in Aedes albopictus cell culture. Genomic sequencing and phylogenetic analysis of predicted ORFs showed that eight of the ten ORFs were most homologous to Peruvian horse sickness virus (PHSV), with amino acid identities of 44.3-73.7 %. The remaining two ORFs, VP3 and VP5, were most similar to Middle Point orbivirus (35.9 %) and Yunnan orbivirus (59.8 %), respectively. Taxonomic classification of orbiviruses is largely based on homology of the major subcore structural protein VP2(T2), encoded by segment 2 for mobuck virus. With only 69.1 % amino acid identity to PHSV, we propose mobuck virus as the prototype of a new species of Orbivirus.
authors.affiliation: Newport Laboratories, 1520 Prairie Drive, Worthington, MN 56187, USA.
authors.forename: Elyse
authors.initials: E
authors.lastname: Cooper
authors.affiliation: Newport Laboratories, 1520 Prairie Drive, Worthington, MN 56187, USA.
authors.forename: Srivishnupriya
authors.initials: S
authors.lastname: Anbalagan
authors.affiliation: Newport Laboratories, 1520 Prairie Drive, Worthington, MN 56187, USA.
authors.forename: Patricia
authors.initials: P
authors.lastname: Klumper
authors.affiliation: Department of Pathobiology and the Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Illinois, Urbana, IL 61801, USA.
authors.forename: Gail
authors.initials: G
authors.lastname: Scherba
authors.affiliation: Newport Laboratories, 1520 Prairie Drive, Worthington, MN 56187, USA.
authors.forename: Randy R
authors.initials: RR
authors.lastname: Simonson
authors.affiliation: Newport Laboratories, 1520 Prairie Drive, Worthington, MN 56187, USA.
authors.forename: Ben M
authors.initials: BM
authors.lastname: Hause
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003670:Deer / Q000821:virology*; D016679:Genome, Viral; D008297:Male; D008923:Missouri; D008969:Molecular Sequence Data; D018163:Orbivirus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D012088:Reoviridae Infections / Q000662:veterinary* / Q000821:virology; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins