title: A cocktail of thermally stable, chemically synthesized capture agents for the efficient detection of anti-gp41 antibodies from human sera.
abstract: We report on a method to improve in vitro diagnostic assays that detect immune response, with specific application to HIV-1. The inherent polyclonal diversity of the humoral immune response was addressed by using sequential in situ click chemistry to develop a cocktail of peptide-based capture agents, the components of which were raised against different, representative anti-HIV antibodies that bind to a conserved epitope of the HIV-1 envelope protein gp41. The cocktail was used to detect anti-HIV-1 antibodies from a panel of sera collected from HIV-positive patients, with improved signal-to-noise ratio relative to the gold standard commercial recombinant protein antigen. The capture agents were stable when stored as a powder for two months at temperatures close to 60(o)C.
authors.affiliation: Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California, United States of America.
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Pfeilsticker
authors.forename: Aiko
authors.initials: A
authors.lastname: Umeda
authors.forename: Blake
authors.initials: B
authors.lastname: Farrow
authors.forename: Connie L
authors.initials: CL
authors.lastname: Hsueh
authors.forename: Kaycie M
authors.initials: KM
authors.lastname: Deyle
authors.forename: Jocelyn T
authors.initials: JT
authors.lastname: Kim
authors.forename: Bert T
authors.initials: BT
authors.lastname: Lai
authors.forename: James R
authors.initials: JR
authors.lastname: Heath
mesh_terms: D000595:Amino Acid Sequence; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D057930:Click Chemistry / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D015483:HIV Antibodies / Q000097:blood / Q000276:immunology*; D015700:HIV Envelope Protein gp41 / Q000276:immunology*; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D006679:HIV Seropositivity / Q000175:diagnosis / Q000276:immunology* / Q000821:virology; D006801:Humans; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*; D011485:Protein Binding / Q000276:immunology; D055550:Protein Stability; D059629:Signal-To-Noise Ratio; D013696:Temperature
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D015483:HIV Antibodies; D015700:HIV Envelope Protein gp41; D010455:Peptides; C514787:gp41 protein, Human immunodeficiency virus 1