title: Preparation of porous styrenics-based monolithic layers for thin layer chromatography coupled with matrix-assisted laser-desorption/ionization time-of-flight mass spectrometric detection.
abstract: Monolithic 50 μm thin poly(4-methylstyrene-co-chloromethylstyrene-co-divinylbenzene) layers attached to 6.0 cm × 3.3 cm glass plates have been prepared, using a thermally initiated polymerization process. These layers had a well-defined porous structure with a globular morphology demonstrated with SEM images and exhibited superhydrophobic properties characterized with a water contact angle of 157°. They were then used for thin-layer chromatography of peptides and proteins fluorescently labeled with fluorescamine. The spots of individual separated compounds were visualized using UV light, and their identities were confirmed with a matrix-assisted laser desorption/ionization time of flight mass spectrometry. The presence of chloromethylstyrene units in the polymer enabled hypercrosslinking via a Friedel-Crafts alkylation reaction, and led to monoliths with much larger surface areas, which were suitable for separations of small dye molecules.
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China; Department of Chemistry, University of California, Berkeley, CA 94720, USA.
authors.forename: Yongqin
authors.initials: Y
authors.lastname: Lv
authors.forename: Zhixing
authors.initials: Z
authors.lastname: Lin
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
authors.forename: Frantisek
authors.initials: F
authors.lastname: Svec
mesh_terms: D002855:Chromatography, Thin Layer / Q000295:instrumentation* / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry / Q000302:isolation & purification; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification; D058105:Polymerization; D011137:Polystyrenes / Q000138:chemical synthesis / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D004396:Coloring Agents; D010455:Peptides; D011137:Polystyrenes; D011506:Proteins
keywords: Hypercrosslinking; Mass spectrometry; Monolithic thin layer; Polystyrene; Thin-layer chromatography