title: A molecular method for assessing the effects of potential contaminants on the rate of zebrafish (Danio rerio) development.
abstract: Monitoring expression of the developmentally regulated genes shh, sox2, and tnnt1 by reverse transcriptase polymerase chain reaction (RT-PCR) allows determination of the rate of embryogenesis in zebrafish (Danio rerio) embryos without direct visual observation. The utility of combining this approach and morphological methods during toxicity studies was demonstrated with embryos developing at either 28.5 °C or 24.5 °C and with embryos exposed to sublethal doses of silver nanoparticles.
authors.affiliation: Department of Biology, Middle Tennessee State University, Murfreesboro, Tennessee, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Beasley
authors.forename: Chris
authors.initials: C
authors.lastname: Graham
authors.forename: Ryan
authors.initials: R
authors.lastname: Otter
authors.forename: Matthew
authors.initials: M
authors.lastname: Elrod-Erickson
mesh_terms: D000818:Animals; D047108:Embryonic Development / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D053823:Hedgehog Proteins / Q000235:genetics; D006358:Hot Temperature; D053768:Metal Nanoparticles / Q000633:toxicity*; D055747:SOX Transcription Factors / Q000235:genetics; D012834:Silver / Q000633:toxicity*; D013312:Stress, Physiological; D020107:Troponin T / Q000235:genetics; D015027:Zebrafish / Q000235:genetics* / Q000254:growth & development; D029961:Zebrafish Proteins / Q000235:genetics
chemical_list: D053823:Hedgehog Proteins; D055747:SOX Transcription Factors; C524053:Shha protein, zebrafish; C544611:Sox2 protein, zebrafish; D020107:Troponin T; D029961:Zebrafish Proteins; D012834:Silver
keywords: Aquatic Toxicology; Biomarkers; Developmental Toxicity; Genetic Toxicology; Nanoparticles