title: Role of microRNA-27a in myoblast differentiation.
abstract: MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs that are critically involved in roles in various aspects of skeletal myogenesis. microRNA miR-27a promotes myoblast proliferation by targeting myostatin, a critical inhibitor of skeletal muscle development, but its mode of action in myoblast differentiation remains unclear. We have found that expression of miR-27a and myostatin were upregulated and downregulated, respectively, during myoblast differentiation. Overexpression of miR-27a increased the number of myosin heavy chain (MHC)-positive cells and upregulated mRNA and protein of MyoD and myogenin. These findings indicate that miR-27a plays a role in enhancing myoblast differentiation.
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, 611130, P. R. China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Chen
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Huang
authors.forename: Daiwen
authors.initials: D
authors.lastname: Chen
authors.forename: Ting
authors.initials: T
authors.lastname: Yang
authors.forename: Guangmang
authors.initials: G
authors.lastname: Liu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D015536:Down-Regulation; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D024510:Muscle Development; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism; D018008:Myogenin / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000378:metabolism; D055435:Myostatin / Q000235:genetics; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs; C540674:Mirn27 microRNA, mouse; D018008:Myogenin; D055435:Myostatin; D018995:Myosin Heavy Chains
keywords: miR-27a; microRNA; myoblast differentiation; myostatin