title: [Effects of Xixin decoction on enzymes related to O-GlcNAc glycosylation of tau proteins in the brain of rats with sporadic Alzheimer's disease].
abstract: OBJECTIVE
  To study the effects of Xixin decoction (XXD) on O-GlcNAc transferase (OGT) and O-GlcNAc glycosidase in O-GlcNAc glycosylation of tau proteins in the brain of rats with sporadic Alzheimer's disease (SAD) and explore the possible mechanism.
  
  
  METHODS
  Male SD rats were randomly divided into sham-operated group, model group, donepezil group, and low-, moderate-, and high-dose XXD groups. After treatment and behavioral test, the rats were sacrificed for detecting the expressions of OGT and O-GlcNAc glycosidase in the brain using immunohistochemistry and Western blotting.
  
  
  RESULTS
  XXD significantly enhanced the expressions of OGT in the hippocampus of SAD rats and lowered the expression of O-GlcNAc glycosidase (P<0.05 or 0.01). OGT and O-GlcNAc glycosidase expressions showed no significant differences between the model group and donepezil group (P>0.05).
  
  
  CONCLUSIONS
  XXD can regulate the expression of OGT and O-GlcNAc glycosidase to enhance O-GlcNAc glycosylation of tau proteins in the hippocampus of SAD rats.
authors.affiliation: Shaanxi University of Chinese Medicine, Xianyang 712046, China. E-mail: diwuyongchang@126.com.
authors.forename: Yongchang
authors.initials: Y
authors.lastname: Diwu
authors.forename: Jinzhou
authors.initials: J
authors.lastname: Tian
authors.forename: Jing
authors.initials: J
authors.lastname: Shi
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000544:Alzheimer Disease / Q000139:chemically induced / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D006031:Glycosylation; D006624:Hippocampus / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin; D016875:tau Proteins / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D016875:tau Proteins; D013311:Streptozocin; D017351:N-Acetylglucosaminyltransferases; C449880:O-GlcNAc transferase; D000118:Acetylglucosaminidase