title: [Human umbilical cord blood mononuclear cell transplantation promotes long-term neurobehavioral functional development of newborn SD rats with hypoxic ischemic brain injury].
abstract: OBJECTIVE
  To explore the effect of human umbilical cord blood mononuclear cells (UCBMC) promoting nerve behavior function and brain tissue recovery of neonatal SD rat with hypoxic ischemic brain injury (HIBI).
  
  
  METHODS
  A modified newborn rat model that had a combined hypoxic and ischemic brain injury as described by Rice-Vannucci was used, early nervous reflex, the Morris water maze and walking track analysis were used to evaluate nervous behavioral function, and brain MRI, HE staining to evaluate brain damage recovery.
  
  
  RESULTS
  Newborn rat Rice-Vannucci model showed significant brain atrophy, obvious hemiplegia of contralateral limbs,e.g right step length [(7.67 ± 0.46) cm vs. (8.22 ± 0.50) cm, F = 1.494] and toe distance [(0.93 ± 0.06) cm vs. (1.12 ± 0.55) cm, F = 0.186] were significantly reduced compared with left side, learning and memory ability was significantly impaired compared with normal control group (P < 0.01); Cliff aversion [(8.44 ± 2.38) s vs.(14.22 ± 5.07) s, t = 4.618] and negative geotaxis reflex time [(7.26 ± 2.00) s vs. (11.76 ± 3.73) s, t = 4.755] on postnatal 14 days of HIBI+ transplantation group were significantly reduced compared with HIBI+NaCl group (P < 0.01) ; the Morris water maze experiment showed escape latency [ (23.11 ± 6.64) s vs. (34.04 ± 12.95) s, t = 3.356] and swimming distance [ (9.12 ± 1.21) cm vs.(12.70 ± 1.53) cm, t = 17.095] of HIBI+transplantation group were significantly reduced compared with those of HIBI+NaCl group (P < 0.01) ; the residual brain volume on postnatal 10 d [ (75.37 ± 4.53)% vs. (67.17 ± 4.08)%, t = -6.017] and 67 d [ (69.05 ± 3.58)% vs.(60.83 ± 3.69)%, t = -7.148]of HIBI+ transplantation group were significantly larger than those of HIBI+NaCl group (P < 0.01); After human UCBMC transplantation, left cortical edema significantly reduced and nerve cell necrosis of HIBI+ transplantation group is not obvious compared with HIBI+NaCl group.
  
  
  CONCLUSIONS
  Human UCBMC intraperitoneal transplantation significantly promoted recovery of injured brain cells and neurobehavioral function development.
authors.affiliation: Department of Pediatrics, the Third Affiliated Hospital of Anhui Medical University, Hefei 230061, China.
authors.forename: Hui-zhi
authors.initials: HZ
authors.lastname: Huang
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Wen
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Jin-hua
authors.initials: JH
authors.lastname: Huang
authors.forename: Shang-quan
authors.initials: SQ
authors.lastname: Liu
authors.forename: Wei-hua
authors.initials: WH
authors.lastname: Ren
authors.forename: Wen-xiang
authors.initials: WX
authors.lastname: Fang
authors.forename: Yin-feng
authors.initials: YF
authors.lastname: Qian
authors.forename: Wei-zhu
authors.initials: WZ
authors.lastname: Hou
authors.forename: Ming-jie
authors.initials: MJ
authors.lastname: Yan
authors.forename: You-heng
authors.initials: YH
authors.lastname: Yao
authors.forename: Wei-Zu
authors.initials: WZ
authors.lastname: Li
authors.forename: Qian-Jin
authors.initials: QJ
authors.lastname: Li
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001284:Atrophy / Q000209:etiology / Q000473:pathology; D001921:Brain / Q000000981:diagnostic imaging / Q000473:pathology*; D002540:Cerebral Cortex / Q000473:pathology; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods*; D004195:Disease Models, Animal; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D020925:Hypoxia-Ischemia, Brain / Q000150:complications / Q000473:pathology / Q000628:therapy*; D007859:Learning Disabilities / Q000209:etiology / Q000517:prevention & control*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000637:transplantation; D008279:Magnetic Resonance Imaging; D008297:Male; D018782:Maze Learning; D009474:Neurons / Q000473:pathology; D011597:Psychomotor Performance; D011859:Radiography; D051381:Rats; D017207:Rats, Sprague-Dawley; D014183:Transplantation, Heterologous