title: [Adsorption and isolation of macroporous resin for three isoflavonoids from puerariae lobatae radix].
abstract: OBJECTIVE
  To study the adsorption function of macroporous resins for the separation and purification of three isoflavonoids (3'-hydroxypuerarin, puerarin, daidzin) in Puerariae Lobatae Radix.
  
  
  METHODS
  The contents of 3'-hydroxypuerarin, puerarin, daidzin were determined by HPLC. D101, AB-8, XDA-5, HP-20 and other five resins were systematically investigated for their adsorption capability, adsorption isothermal curve, adsorption and elution properties with static and dynamic experiments.
  
  
  RESULTS
  Experimental results showed that macroporous resin XDA-5 owned optimum adsorption and elution parameters. Optimal conditions for adsorption and elution the three components were: per 1 mL solution contained Puerariae Lobatae Radix 0.5 - 0.8 g, flow rate 2BV/h, adsorbed 3'-hydroxypuerarin, puerarin and daidzin could be desorbed by alcohol at flow rate 1BV/h.
  
  
  CONCLUSIONS
  The purity of 3'-hydroxypuerarin, puerarin and daidzin of the product obtained from Puerariae Lobatae Radix with optimum technology is 23.39%, 52.09% and 19.21%, respectively.
authors.affiliation: The First Fffiliated Hospital of Jinan University, Guangzhou 510632, China. jnulyc@yahoo.com.cn
authors.forename: Yun-Hui
authors.initials: YH
authors.lastname: Cui
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Fan
authors.forename: Ke
authors.initials: K
authors.lastname: Yang
mesh_terms: D000327:Adsorption; D002853:Chromatography, Liquid; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification*; D016014:Linear Models; D018517:Plant Roots / Q000737:chemistry; D016062:Porosity; D029908:Pueraria / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D007529:Isoflavones; D012117:Resins, Synthetic; C013908:daidzin; C033607:puerarin