title: Contribution of very low-density lipoprotein triglyceride fatty acids to postabsorptive free fatty acid flux in obese humans.
abstract: OBJECTIVE
  In the fasting state, plasma free fatty acids (FFA) are thought to derive almost exclusively from adipose tissue lipolysis. However, there are mixed reports as to whether the spillover of fatty acids (FA) from very low-density lipoprotein triglyceride (VLDL-TG) hydrolysis contributes significantly to the plasma FFA pool. Because substantial VLDL-TG fatty acid spillover into the plasma FFA pool would profoundly impact the interpretation of isotope dilution measures of FFA flux, we investigated the contribution of VLDL-TG spillover to plasma FFA appearance.
  
  
  METHODS
  Eighteen obese adults (15 women) participated in these studies. Each volunteer received a primed, continuous infusion of their own ex-vivo labeled ([1-(14)C]triolein) VLDL-TG and a continuous infusion of [U-(13)C]oleate (8 nmol · kg fat free mass(-1) · min(-1)) to measure VLDL-TG and FFA rate of appearance (Ra), respectively. The presence of (14)C-oleate in the plasma FFA-oleate pool was used to calculate the contribution of spillover from VLDL-TG-oleate to the plasma FFA-oleate Ra.
  
  
  RESULTS
  The spillover rate of VLDL-TG-oleate into plasma FFA-oleate was 6 ± 2 μmol/min (7% ± 2% of [(14)C]oleate from VLDL-TG) and FFA-oleate flux was 240 ± 61 μmol/min. Thus, only 3% ± 1% of total plasma FFA-oleate appearance could be accounted for by VLDL-TG spillover.
  
  
  CONCLUSIONS
  The contribution of VLDL-TG spillover to the total plasma FFA pool is negligible and will not materially affect the interpretation of FFA flux measures as an index of adipose tissue lipolysis.
authors.affiliation: Endocrine Research Unit, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Nikki C
authors.initials: NC
authors.lastname: Bush
authors.forename: Jessica M
authors.initials: JM
authors.lastname: Triay
authors.forename: Nicola W
authors.initials: NW
authors.lastname: Gathaiya
authors.forename: Kazanna C
authors.initials: KC
authors.lastname: Hames
authors.forename: Michael D
authors.initials: MD
authors.lastname: Jensen
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D005230:Fatty Acids, Nonesterified / Q000097:blood / Q000378:metabolism*; D005260:Female; D006801:Humans; D008066:Lipolysis; D008079:Lipoproteins, VLDL / Q000097:blood / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood / Q000378:metabolism*
chemical_list: D005230:Fatty Acids, Nonesterified; D008079:Lipoproteins, VLDL; D014280:Triglycerides; C030106:very low density lipoprotein triglyceride
keywords: BMI; FA; FFA; Hypertriglyceridemia; Isotope dilution; SA; TG; VLDL; [(14)C]triolein; [U-(13)C]oleate; body mass index; fatty acid; free fatty acids; specific activity; triglyceride; very low density lipoprotein