title: Differential protein expression of lymph node metastases of papillary thyroid carcinoma harboring the BRAF mutation.
abstract: BACKGROUND
  The prognostic role of the T1799A BRAF mutation is controversial. We investigated the protein expression in papillary thyroid carcinoma (PTCs) samples harboring the specific mutation using proteomic tools.
  
  
  METHODS
  We performed two-dimensional gel electrophoresis to identify differential protein expression regarding lymph node metastasis (LNM). Proteins were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Immunohistochemical staining was performed for 38 PTCs harboring the mutation. We validated the association between these proteins and clinicopathological factors in a test set of 121 PTCs.
  
  
  RESULTS
  The expression of vimentin was increased in PTCs with LNM, but the one for HSP60, SOD2 and PEBP1 was increased in samples without LNM. HSP60 protein was up-regulated in PTCs without LNM (84.2% vs. 36.8%. p=0.003) and in PTCs without LNM harboring the mutation (58% vs. 41.8%. p=0.003) in the test set as shown by immunohistochemical staining.
  
  
  CONCLUSIONS
  HSP60 protein expression may inhibit LNM in PTCs harboring the BRAF mutation and may be a useful prognostic marker.
authors.affiliation: Division of Endocrine Surgery, Department of Surgery, Asan Medical Center, 88, Olympic-ro 43-gil, Songpao-gu, Seoul, 138-736, Republic of Korea. surgeonckw@amc.seoul.kr.
authors.forename: Won Seo
authors.initials: WS
authors.lastname: Park
authors.forename: Ki-Wook
authors.initials: KW
authors.lastname: Chung
authors.forename: Min Sun
authors.initials: MS
authors.lastname: Young
authors.forename: Seok-Ki
authors.initials: SK
authors.lastname: Kim
authors.forename: You Jin
authors.initials: YJ
authors.lastname: Lee
authors.forename: Eun Kyung
authors.initials: EK
authors.lastname: Lee
mesh_terms: D000328:Adult; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D002291:Carcinoma, Papillary / Q000378:metabolism* / Q000556:secondary; D018834:Chaperonin 60 / Q000378:metabolism*; D004252:DNA Mutational Analysis; D005260:Female; D015870:Gene Expression; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000378:metabolism*; D020125:Mutation, Missense; D049488:Phosphatidylethanolamine Binding Protein / Q000378:metabolism; D011379:Prognosis; D020543:Proteome / Q000378:metabolism; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D013482:Superoxide Dismutase / Q000378:metabolism; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology; D000099156:Superoxide Dismutase 2
chemical_list: D018834:Chaperonin 60; D024101:Mitochondrial Proteins; D049488:Phosphatidylethanolamine Binding Protein; D020543:Proteome; D048493:Proto-Oncogene Proteins B-raf; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2; C060293:HSPD1 protein, human; C511482:PEBP1 protein, human; C482119:BRAF protein, human
keywords: BRAF mutation; Thyroid neoplasms; chaperonin 60; lymph node metastases; proteomics