title: Polygalacturonases from Moniliophthora perniciosa are regulated by fermentable carbon sources and possible post-translational modifications.
abstract: We report the first molecular and in silico analysis of Monilophthora perniciosa polygalacturonases (PGs). Three MpPG genes (MpPG1, MpPG2 and MpPG3) were identified and analyzed at transcriptional level, by RT-qPCR, in dikaryotic M. perniciosa mycelium grown on solid-bran based medium and on liquid medium supplemented with different fermentable and non-fermentable carbon sources. The MpPG genes presented different expression patterns suggesting different individual regulation. However, all are mainly regulated by fermentable carbon sources (galactose and mannose). The integrated analysis of PG gene expression and systems biology (using MpG1 and MpG2 orthologs in Neurospora crassa, named NCU06961 and NCU02369, respectively) allowed identifying some possible mechanism of protein regulation during the necrotrophic fungal phase. MpPG1-NCU06961 and MpPG2-NCU02369 directly or indirectly interacted with central and highly connected proteins involved in protein synthesis and protein regulation associated to post-translational modifications, in cell wall metabolism, and in cellular metabolism related to energy production. This analysis also allowed the identification of key proteins for further studies of M. perniciosa development and/or for disease management, such as MpPG2, a pectin methylesterase, an acetolactate synthase and the small ubiquitin-like modifier SMT3-like.
authors.affiliation: Universidade Estadual de Santa Cruz (UESC), Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Rodovia Ilhéus-Itabuna, km 16, 45662-900 Ilhéus-BA, Brazil.
authors.forename: Heliana
authors.initials: H
authors.lastname: Argôlo Santos Carvalho
authors.forename: Edson Mario
authors.initials: EM
authors.lastname: de Andrade Silva
authors.forename: Stenio
authors.initials: S
authors.lastname: Carvalho Santos
authors.forename: Fabienne
authors.initials: F
authors.lastname: Micheli
mesh_terms: D000363:Agaricales / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D002099:Cacao / Q000382:microbiology; D004271:DNA, Fungal / Q000032:analysis; D005285:Fermentation; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D008358:Mannose / Q000378:metabolism*; D008969:Molecular Sequence Data; D025282:Mycelium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D009492:Neurospora crassa / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D011096:Polygalacturonase / Q000235:genetics* / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D011096:Polygalacturonase; D008358:Mannose; D005690:Galactose
keywords: Acetolactate synthase; BCAA; Energy metabolism; GO; MM; MOX; ORF; PG; PL; PME; PPI; PTM; Pectin methylesterase; Small ubiquitin-like modifier; Systems biology; Witches’ broom disease; branched chain amino acid; gene ontology; methanol oxidase; mineral medium; open reading frame; pectate lyase; pectin methylesterase; polygalacturonase; post-translational modification; protein–protein interaction