title: Emodin attenuates high glucose-induced TGF-β1 and fibronectin expression in mesangial cells through inhibition of NF-κB pathway.
abstract: The activation of nuclear factor-κB (NF-κB) and the subsequent overexpression of its downstream targets transforming growth factor-β1 (TGF-β1) and fibronectin (FN) are among the hallmarks for the progressive diabetic nephropathy. Our previous studies demonstrated that emodin ameliorated renal injury and inhibited extracellular matrix accumulation in kidney and mesangial cells under diabetic condition. However, the molecular mechanism has not been fully elucidated. Here, we showed that emodin significantly attenuated high glucose-induced NF-κB nuclear translocation in mesangial cells. Interestingly, emodin also inhibited the DNA-binding activity and transcriptional activity of NF-κB. Furthermore, NF-κB-mediated TGF-β1 and FN expression was significantly decreased by emodin. These results demonstrated that emodin suppressed TGF-β1 and FN overexpression through inhibition of NF-κB activation, suggesting that emodin-mediated inhibition of the NF-κB pathway could protect against diabetic nephropathy.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Guangxi Medical University, Nanning, Guangxi, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zeng
authors.forename: Teng
authors.initials: T
authors.lastname: Wu
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Yang
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.forename: Tian
authors.initials: T
authors.lastname: Lan
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004642:Emodin / Q000494:pharmacology*; D005353:Fibronectins / Q000096:biosynthesis*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005947:Glucose / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D050527:Mesangial Cells / Q000187:drug effects* / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013329:Structure-Activity Relationship; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis*
chemical_list: D005353:Fibronectins; D016328:NF-kappa B; D053773:Transforming Growth Factor beta1; D005947:Glucose; D004642:Emodin
keywords: DMEM; Dulbecco's modified Eagle's medium; ECM; EMSA; Emodin; FN; High glucose; MCs; Mesangial cells; NF-κB; TGF-β1; electrophoretic mobility shift assay; extracellular matrix; fibronectin; mesangial cells; nuclear factor-κB; transforming growth factor-β1