title: Development and validation of an LC-ESI-MS/MS method for the quantitation of hemslecin A in rhesus monkey plasma and its application in pharmacokinetics.
abstract: In this study, a new LC-ESI-MS/MS-based method was validated for the quantitation of hemslecin A in rhesus monkey plasma using otophylloside A as internal standard (IS). Hemslecin A and the IS were extracted from rhesus monkey plasma using liquid-liquid extraction as the sample clean-up procedure, and were subjected to chromatography on a Phenomenex Luna CN column (150 × 2.0 mm, 3.0 µm) with the mobile phase consisting of methanol and 0.02 mol/mL ammonium acetate (55:45, v/v) at a flow rate of 0.2 mL/min. Detection was performed on an Agilent G6410B tandem mass spectrometer by positive ion electrospray ionization in multiple reaction monitoring mode, monitoring the transitions m/z 580.5 [M + NH4 ](+)  → 503.4 and m/z 518.2 [M + NH4 ](+)  → 345.0 for hemslecin A and IS, respectively. The assay was linear over the concentration range of 0.5-200 ng/mL and was successfully applied to a pharmacokinetic study in rhesus monkeys.
authors.affiliation: Department of Pharmacy, Han Dan Central Hospital, No. 15, South Zhong-Hua Street, Handan, Hebei, 056001, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Bai
authors.forename: Hong-Liang
authors.initials: HL
authors.lastname: Li
authors.forename: Jian-Chang
authors.initials: JC
authors.lastname: He
authors.forename: Gong-Hao
authors.initials: GH
authors.lastname: He
authors.forename: En-Fu
authors.initials: EF
authors.lastname: Feng
authors.forename: Yue-Qiong
authors.initials: YQ
authors.lastname: Liu
authors.forename: Pan-Pan
authors.initials: PP
authors.lastname: Shi
authors.forename: Gui-Li
authors.initials: GL
authors.lastname: Xu
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D054728:Cucurbitacins / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D004355:Drug Stability; D057230:Limit of Detection; D008253:Macaca mulatta; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D054728:Cucurbitacins
keywords: LC-ESI-MS/MS; hemslecin A; pharmacokinetics; rhesus monkey plasma