title: Multiplex detection of DNA mutations by the fluorescence fingerprint spectrum technique.
abstract: A fingerprint spectrum technique that utilizes cationic conjugated-polymer-based fluorescence resonance energy transfer (FRET) is used for multiplex detection of DNA mutations. This method detects as low as 5 % mutation of the total DNA. Ten PIK3CA mutations originating from 30 clinical breast cancer samples could be detected.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P.R. China).
authors.forename: Jinzhao
authors.initials: J
authors.lastname: Song
authors.forename: Jiangyan
authors.initials: J
authors.lastname: Zhang
authors.forename: Fengting
authors.initials: F
authors.lastname: Lv
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Cheng
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Liheng
authors.initials: L
authors.lastname: Feng
authors.forename: Libing
authors.initials: L
authors.lastname: Liu
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis* / Q000235:genetics* / Q000378:metabolism; D016172:DNA Fingerprinting / Q000379:methods; D004252:DNA Mutational Analysis / Q000379:methods*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D009154:Mutation
chemical_list: D004247:DNA
keywords: DNA mutation; FRET; biosensors; conjugated polymers; fingerprint spectrum