title: Investigation of growth conditions for the expansion of porcine mesenchymal stem cells on microcarriers in stirred cultures.
abstract: The extensive use of mesenchymal stem cells (MCS) in tissue engineering and cell therapy increases the necessity to improve their expansion. Among these, porcine MCS are valuable models for tissue engineering and are classically expanded in static T-flasks. In this work, different processes of stirred cultures were evaluated and compared. First, the effect of glucose, glutamine, antioxidant, and growth factors concentrations on porcine MSC expansion were analyzed in a suitable medium by performing kinetic studies. Results showed that a lower glucose concentration (5.5 mM) enabled to increase maximal cell concentration by 40 % compared with a higher one (25 mM), while addition of 2 to 6 mM of glutamine increased maximal cell concentration by more than 25 % compared with no glutamine supplementation. Moreover, supplementation with 1 μM thioctic acid increased maximal cell concentration by 40 % compared with no supplementation. Using this adapted medium, microcarriers cultures were performed and compared with T-flasks expansion. Porcine MSC were shown to be able to proliferate on the five types of microcarriers tested. Moreover, cultures on Cytodex 1, Cytopore 2, and Cultispher G exhibited a MSC growth rate more than 40 % higher compared with expansion in T-flasks, while MSC metabolism was similar.
authors.affiliation: Laboratoire Réactions et Génie des Procédés, CNRS UMR 7274, Université de Lorraine, 2 avenue de la forêt de Haye, TSA 40602, 54518, Vandœuvre-lès-Nancy Cedex, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Ferrari
authors.forename: Eric
authors.initials: E
authors.lastname: Olmos
authors.forename: Frédérique
authors.initials: F
authors.lastname: Balandras
authors.forename: Nguyen
authors.initials: N
authors.lastname: Tran
authors.forename: Isabelle
authors.initials: I
authors.lastname: Chevalot
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Guedon
authors.forename: Annie
authors.initials: A
authors.lastname: Marc
mesh_terms: D000641:Ammonia / Q000378:metabolism; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D005973:Glutamine / Q000494:pharmacology; D007700:Kinetics; D019344:Lactic Acid / Q000096:biosynthesis; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008863:Microspheres; D039902:Multipotent Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D034421:Sus scrofa
chemical_list: D003470:Culture Media; D005973:Glutamine; D019344:Lactic Acid; D000641:Ammonia; D005947:Glucose