title: In-vitro experimental evaluation of skin-to-surface recovery of four bacterial species by antibacterial and non-antibacterial medical examination gloves.
abstract: BACKGROUND
  The number of bacteria recovered from a stainless steel coupon after touching a pigskin substrate with an examination glove coated on its outside with polyhexanide (PHMB), as compared to the number of bacteria recovered in the same manner with non-coated control gloves was evaluated.
  
  
  METHODS
  Suspensions containing 1 × 109 colony-forming units of 4 clinically relevant bacterial species (Enterococcus faecium ATCC #51559; Escherichia coli ATCC #25922; Klebsiella pneumoniae ATCC #4352; and Staphylococcus aureus ATCC #33591) were used to contaminate Gamma-irradiated pigskin substrates. Bacterial recoveries from the pigskin substrate, stainless steel coupons, and each glove swatch were performed. A difference in the bacterial recovery from the stainless steel coupons after touching with coated and uncoated control gloves was measured.
  
  
  RESULTS
  For E. faecium, the coated glove showed a reduction of 4.63 log10 cfu recovery, when compared to control gloves. For E. coli, the coated glove showed 5.48 log10 cfu, for K. pneumoniae 5.03 log10 cfu, and for S. aureus 5.72 log10 cfu recovery, when compared to the non-coated control glove.
  
  
  CONCLUSIONS
  An in-vitro experiment designed to mimic cross-contamination of clinically relevant bacteria in a simulated healthcare setting following glove contact with a contaminated biological surface and cross-transfer to a stainless steel surface has demonstrated that an examination glove coated on its outside surface with PHMB was able to reduce bacterial recovery from a contaminated surface by > 4 log10 cfu, compared to a control non-coated examination glove. These elaborated results may encourage further clinical investigation on the clinical impact of an antibacterial examination glove.
authors.affiliation: Department for Hospital Hygiene, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria. ojan.assadian@meduniwien.ac.at.
authors.forename: Johannes
authors.initials: J
authors.lastname: Leitgeb
authors.forename: Rupert
authors.initials: R
authors.lastname: Schuster
authors.forename: Aik-Hwee
authors.initials: AH
authors.lastname: Eng
authors.forename: Bit-New
authors.initials: BN
authors.lastname: Yee
authors.forename: Yee-Peng
authors.initials: YP
authors.lastname: Teh
authors.forename: Verena
authors.initials: V
authors.lastname: Dosch
authors.forename: Ojan
authors.initials: O
authors.lastname: Assadian