title: Breeding based remobilization of Tol2 transposon in Xenopus tropicalis.
abstract: Xenopus is a powerful model for studying a diverse array of biological processes. However, despite multiple methods for transgenesis, relatively few transgenic reporter lines are available and commonly used. Previous work has demonstrated that transposon based strategies are effective for generating transgenic lines in both invertebrate and vertebrate systems. Here we show that the Tol2 transposon can be remobilized in the genome of X. tropicalis and passed through the germline via a simple breeding strategy of crossing transposase expressing and transposon lines. This remobilization system provides another tool to exploit transgenesis and opens new opportunities for gene trap and enhancer trap strategies.
authors.affiliation: Program in Vertebrate Developmental Biology, Department of Pediatrics and Genetics, Yale University School of Medicine, New Haven, Connecticut, United States of America.
authors.forename: Maura A
authors.initials: MA
authors.lastname: Lane
authors.forename: Megan
authors.initials: M
authors.lastname: Kimber
authors.forename: Mustafa K
authors.initials: MK
authors.lastname: Khokha
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D001947:Breeding; D003433:Crosses, Genetic; D004251:DNA Transposable Elements / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D008856:Microscopy, Fluorescence; D008957:Models, Genetic; D016254:Mutagenesis, Insertional; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019895:Transposases / Q000235:genetics* / Q000378:metabolism; D014981:Xenopus / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D049452:Green Fluorescent Proteins; D019895:Transposases