title: Regional thalamic neuropathology in patients with hippocampal sclerosis and epilepsy: a postmortem study.
abstract: OBJECTIVE
  Clinical, experimental, and neuroimaging data all indicate that the thalamus is involved in the network of changes associated with temporal lobe epilepsy (TLE), particularly in association with hippocampal sclerosis (HS), with potential roles in seizure initiation and propagation. Pathologic changes in the thalamus may be a result of an initial insult, ongoing seizures, or retrograde degeneration through reciprocal connections between thalamic and limbic regions. Our aim was to carry out a neuropathologic analysis of the thalamus in a postmortem (PM) epilepsy series, to assess the distribution, severity, and nature of pathologic changes and its association with HS.
  
  
  METHODS
  Twenty-four epilepsy PM cases (age range 25-87 years) and eight controls (age range 38-85 years) were studied. HS was classified as unilateral (UHS, 11 cases), bilateral (BHS, 4 cases) or absent (No-HS, 9 cases). Samples from the left and right sides of the thalamus were stained with cresyl violet (CV), and for glial firbillary acidic protein (GFAP) and synaptophysin. Using image analysis, neuronal densities (NDs) or field fraction staining values (GFAP, synaptophysin) were measured in four thalamic nuclei: anteroventral nucleus (AV), lateral dorsal nucleus (LD), mediodorsal nucleus (MD), and ventrolateral nucleus (VL). The results were compared within and between cases.
  
  
  RESULTS
  The severity, nature, and distribution of thalamic pathology varied between cases. A pattern that emerged was a preferential involvement of the MD in UHS cases with a reduction in mean ND ipsilateral to the side of HS (p = 0.05). In UHS cases, greater field fraction values for GFAP and lower values for synaptophysin and ND were seen in the majority of cases in the MD ipsilateral to the side of sclerosis compared to other thalamic nuclei. In addition, differences in the mean ND between classical HS, atypical HS, and No-HS cases were noted in the ipsilateral MD (p < 0.05), with lower values observed in HS.
  
  
  CONCLUSIONS
  Our study demonstrates that stereotypical pathologic changes, as seen in HS, are not clearly defined in the thalamus. This may be partly explained by the heterogeneity of our PM study group. With quantitation, there is some evidence for preferential involvement of the MD, suggesting a potential role in TLE, which requires further investigation.
authors.affiliation: Department of Clinical and Experimental Epilepsy, Institute of Neurology and National Hospital for Neurology and Neurosurgery, London, United Kingdom.
authors.forename: Barah
authors.initials: B
authors.lastname: Sinjab
authors.forename: Lillian
authors.initials: L
authors.lastname: Martinian
authors.forename: Sanjay M
authors.initials: SM
authors.lastname: Sisodiya
authors.forename: Maria
authors.initials: M
authors.lastname: Thom
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D004827:Epilepsy / Q000473:pathology*; D006624:Hippocampus / Q000473:pathology*; D006801:Humans; D008875:Middle Aged; D012598:Sclerosis; D013788:Thalamus / Q000473:pathology*
keywords: Gliosis; Hippocampal sclerosis; Mediodorsal nucleus; Thalamus