title: Dynamic PET with (18)F-Deoxyglucose (FDG) and quantitative assessment with a two-tissue compartment model reflect the activity of glucose transporters and hexokinases in patients with colorectal tumors.
abstract: Dynamic PET (dPET) with (18)F-Deoxyglucose (FDG) provides quantitative information about distribution of the tracer in a predefined volume over time. A two-tissue compartment model can be used to obtain quantitative data regarding transport of FDG into and out of the cells, phosphorylation and dephosphorylation rate of intracellular FDG, and fractional blood volume in the target volume, also named vessel density. Aim of the study was the correlation of glucose transporters expression and hexokinases with the corresponding compartment parameters.Patients with colorectal tumors were examined with dynamic PET prior to surgery. Afterwards, tumor samples were obtained during surgery and gene expression was assessed using gene arrays. The dynamic PET data were evaluated to quantify the parameters of a two tissue compartment model for colorectal tumors using a Volume-of-Interest (VOI) technique. A multiple correlation/regression analysis was performed using glucose transporters as independent variables and k1 as the dependent variable. A correlation of r=0.7503 (p=0.03) was obtained for the transporters SLC2A1, SLC2A2, SLC2A4, SLC2A8, SLC2A9, SLC2A10 and k1. The correlation of r=0.7503 refers to an explained variance of data of 56.30 %, therefore more than 50 % of data changes are associated with the gene expression. An analysis of the hexokinases HK1-HK3 and k3 revealed a correlation coefficient of r=0.6093 (p=0.04), which is associated with an explained variance of 37.12 %. Therefore, parameters k1 and k3 reflect gene activity. The results demonstrate that k1 and k3 of the two-tissue compartment model are correlated with glucose transporters and hexokinases.
authors.affiliation: Clinical Cooperation Unit Nuclear Medicine, German Cancer Research Center Heidelberg, Germany.
authors.forename: Ludwig G
authors.initials: LG
authors.lastname: Strauss
authors.forename: Dirk
authors.initials: D
authors.lastname: Koczan
authors.forename: Sven
authors.initials: S
authors.lastname: Klippel
authors.forename: Leyun
authors.initials: L
authors.lastname: Pan
authors.forename: Stefan
authors.initials: S
authors.lastname: Willis
authors.forename: Christos
authors.initials: C
authors.lastname: Sachpekidis
authors.forename: Antonia
authors.initials: A
authors.lastname: Dimitrakopoulou-Strauss
keywords: Dynamic PET; compartment model; glucose transporter; hexokinase