title: Molecular characterization of Candida spp. isolates from patients with bloodstream infections.
abstract: BACKGROUND
  The aim of this study was to conduct an epidemiological study comparing the genetic similarity of yeasts isolated from blood cultures.
  
  
  METHODS
  Random amplification of polymorphic DNA (RAPD) techniques were used for the Candida samples obtained from patients at the Hospital Universitário da Universidade Federal do Mato Grosso do Sul (HU/UFMS) in Campo Grande, State of Mato Grosso do Sul, Brazil, from 1998-2000.
  
  
  RESULTS
  The most frequently isolated species was Candida albicans (45.8%). DNA amplification from genomic yeast isolates indicated a genetic similarity of over 90%.
  
  
  CONCLUSIONS
  The RAPD profiles obtained were able to differentiate between the isolated Candida species, thereby suggesting that the method might be useful in epidemiological studies.
authors.forename: Paula Cristhina Niz
authors.initials: PC
authors.lastname: Xavier
authors.forename: Marilene Rodrigues
authors.initials: MR
authors.lastname: Chang
authors.forename: Claudete Rodrigues
authors.initials: CR
authors.lastname: Paula
authors.forename: Flávia Emi
authors.initials: FE
authors.lastname: Matsumoto
authors.forename: Marise Dutra
authors.initials: MD
authors.lastname: Asensi
authors.forename: Maria de Fátima Cepa
authors.initials: Mde F
authors.lastname: Matos
authors.forename: Renato
authors.initials: R
authors.lastname: Andreotti
mesh_terms: D001938:Brazil / Q000453:epidemiology; D002175:Candida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D002177:Candidiasis / Q000453:epidemiology / Q000382:microbiology*; D004271:DNA, Fungal / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D016469:Fungemia / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D016533:Mycological Typing Techniques; D019105:Random Amplified Polymorphic DNA Technique; D012189:Retrospective Studies
chemical_list: D004271:DNA, Fungal