title: The effect of 17-methoxyl-7-hydroxy-benzene-furanchalcone on NF-κB and the inflammatory response during myocardial ischemia reperfusion injury in rats.
abstract: The aim of this study was to investigate the effect of 17-methoxyl-7-hydroxy-benzene-furanchalcone (MHBFC) on nuclear factor-kappa-binding (NF-κB) and the inflammatory response in rats with myocardial ischemia reperfusion injury (MI/RI). Sprague-Dawley rats were randomly divided into 7 groups, and the rat MI/RI model was established by the ligation of the left anterior descending for 30 minutes followed by ligation release for 1 hour. Areas of myocardial infarction were measured using Evans blue-2,3,5-Triphenyltetrazolium chloride (TTC) staining. Levels of malondialdehyde, glutathione peroxidase, and total superoxide dismutase were assessed. Release of interleukin-1β (IL-1β), interleukin-6 (IL-6), and interleukin-10 (IL-10) was measured by means of an enzyme-linked immunosorbent assay. NF-κBp65 and intercellular adhesion molecule-1 protein expression and caspase-3 and adenine nucleotide translocator-1 messenger RNA expression were evaluated by immunohistochemistry and reverse transcription polymerase chain reaction, respectively. Pretreatment with MHBFC decreased the infarction areas, the malondialdehyde, IL-1β and IL-6 levels, the expression of caspase-3, NF-κBp65, and intercellular adhesion molecule-1. Further, MHBFC increased total superoxide dismutase and glutathione peroxidase activities, the release of IL-10, and the expression of adenine nucleotide translocator-1 messenger RNA compared with the results of the model group. The experiment showed that MHBFC protected the heart against MI/RI possibly by reducing lipid peroxidation damage while inhibiting the activity of NF-κBp65 and the inflammatory response.
authors.affiliation: Department of Pharmacology, Guangxi Medical University, Nanning, China.
authors.forename: Xingmei
authors.initials: X
authors.lastname: Liang
authors.forename: Jianchun
authors.initials: J
authors.lastname: Huang
authors.forename: Xing
authors.initials: X
authors.lastname: Lin
authors.forename: Feizhang
authors.initials: F
authors.lastname: Qin
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Wen
authors.forename: Chunxia
authors.initials: C
authors.lastname: Chen
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Li
authors.forename: Wenyi
authors.initials: W
authors.lastname: Ge
authors.forename: Renbin
authors.initials: R
authors.lastname: Huang
mesh_terms: D033741:Adenine Nucleotide Translocator 1 / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D047188:Chalcones / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D033641:Millettia / Q000737:chemistry; D009203:Myocardial Infarction / Q000473:pathology; D015428:Myocardial Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D009206:Myocardium / Q000473:pathology; D016328:NF-kappa B / Q000096:biosynthesis*; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000378:metabolism; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: C578356:17-methoxyl-7-hydroxy-benzene-furanchalcone; D033741:Adenine Nucleotide Translocator 1; D047188:Chalcones; D016207:Cytokines; D016328:NF-kappa B; D051996:Transcription Factor RelA; D018799:Intercellular Adhesion Molecule-1; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D053148:Caspase 3