title: Reciprocal relationship of Tn/NF-κB and sTn as an indicator of the prognosis of oral squamous cell carcinoma.
abstract: OBJECTIVE
  In order to determine whether the expression of tumour-associated carbohydrate antigens (Tn/sTn) and a representative inflammation marker, nuclear factor-κB (NF-κB), is associated with the invasiveness of oral squamous cell carcinoma (OSCC), this study has attempted to investigate the correlation of the aforementioned markers with the well-established invasive pattern grading score (IPGS) and clinicopathological parameters.
  
  
  RESULTS
  Specimens from 143 OSCC patients with classified clinicopathological parameters and IPGS were stained immunohistochemically using anti-Tn, sTn and NF-κB antibodies. Our results showed that the expression of both Tn and NF-κB was correlated positively with staging (P = 0.036; P = 0.015), recurrence (P < 0.001; P < 0.001) and distant metastasis (P = 0.005; P = 0.009), as well as with IPGS, while the expression of sTn was correlated inversely. In addition, poor survival was associated with overexpression of Tn and NF-κB but not with expression of sTn.
  
  
  CONCLUSIONS
  Our results indicate that a reciprocal relationship between Tn and sTn expression may serve as a reliable indicator for OSCC prognostic evaluation. In addition, expression of Tn rather than sTn may play an important role in deeply invasive OSCC via regulation of NF-κB signalling.
authors.affiliation: Graduate Institute of Life Sciences, National Defense Medical Centre, Taipei, Taiwan; Department of Biochemistry, Medical College, Taipei Medical University, Taipei, Taiwan.
authors.forename: Chi-Yu
authors.initials: CY
authors.lastname: Lin
authors.forename: Jar-Yi
authors.initials: JY
authors.lastname: Ho
authors.forename: Meng-Ti
authors.initials: MT
authors.lastname: Hsieh
authors.forename: Hsiao-Ling
authors.initials: HL
authors.lastname: Chiang
authors.forename: Jei-Min
authors.initials: JM
authors.lastname: Chuang
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Whang-Peng
authors.forename: Yun-Ching
authors.initials: YC
authors.lastname: Chang
authors.forename: Yi-Han
authors.initials: YH
authors.lastname: Tseng
authors.forename: Su-Feng
authors.initials: SF
authors.lastname: Chen
authors.forename: Shin
authors.initials: S
authors.lastname: Nieh
authors.forename: Jaulang
authors.initials: J
authors.lastname: Hwang
mesh_terms: D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology*; D016328:NF-kappa B / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D011379:Prognosis; D015398:Signal Transduction
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D016328:NF-kappa B; C040286:Tn antigen; C055491:sialosyl-Tn antigen
keywords: invasive pattern grading score; nuclear factor-κB; oral squamous cell carcinoma; tumour-associated carbohydrate antigen