title: [Assessment and application of a molecular diagnostic method on the detection of four types of diarrheagenic Escherichia coli].
abstract: OBJECTIVE
  To establish and evaluate a molecular diagnostic method for routine monitoring of four types of diarrheagenic Escherichia (E.) coli (DEC)and to study the distribution of four types of DEC isolated from diarrheal patients in Shanghai.
  
  
  METHODS
  DEC-PCR standard operation procedure(SOP)had been developed for DEC detection and isolation, using the Statens Serum Institute (SSI) DEC PCR kits with multiplex PCR technique after verification tests on reference strains. Diarrhea specimens from 3 clinical hospitals in Shanghai were tested from June to September, 2012.
  
  
  RESULTS
  Specificity of the PCR kit was 100% by verification on the 26 DEC reference strains. A total number of 218 DEC isolates, including 181 fermented lactose and 37 unfermented lactose were identified from the 1887 stool specimens of diarrhea patients, with positive rate as 11.6%. The most common pathogen(54.1%, 118/218)was enteropathogenic E. coli(EPEC), followed by enterotoxigenic E. coli(ETEC, 41.3%, 90/218), enteroinvasive E. coli (EIEC, 4.1%, 9/ 218) and Shigatoxin-producing E. coli(STEC, 0.5%, 1/218)in addition to 18 Shigella isolates. ETEC dominated in diarrhea patients with foreign residency, as well as 1/3 were perinatal stage of neonatal ETEC of all diarrhea cases under the age of 5, while EPEC dominated in the Chinese diarrhea patients especially among young kids under the age of 2.
  
  
  CONCLUSIONS
  Data was reliable after assessment on this molecular diagnostics and seperation procedures used for the routine monitoring on four types of DEC, while the diagnosis and reference ability of DEC regarding the laboratories net-working on food-borne pathogens need to be built up and improved.
authors.affiliation: Center of Hygiene Inspection of Changning District, Shanghai 200051, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Huang
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.forename: Jia-yin
authors.initials: JY
authors.lastname: Guo
authors.forename: Xiao-lan
authors.initials: XL
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: Hou
authors.forename: Ai-min
authors.initials: AM
authors.lastname: Wang
authors.forename: Chuan-qing
authors.initials: CQ
authors.lastname: Wang
authors.forename: Hui-ming
authors.initials: HM
authors.lastname: Jin
authors.forename: Xue-bin
authors.initials: XB
authors.lastname: Xu
authors.forename: Jia-yu
authors.initials: JY
authors.lastname: Hu
authors.forename: Xian-ming
authors.initials: XM
authors.lastname: Shi
authors.forename: Lu
authors.initials: L
authors.lastname: Ran
authors.forename: Biao
authors.initials: B
authors.lastname: Kan
mesh_terms: D000293:Adolescent; D000328:Adult; D002681:China / Q000453:epidemiology; D003967:Diarrhea / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000453:epidemiology; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D057089:Pathology, Molecular; D018571:Sentinel Surveillance