title: Mass Western for absolute quantification of target proteins and considerations about the instrument of choice.
abstract: The Mass Western describes the absolute quantification of proteins based on stable isotope labeled integral standard peptides and liquid chromatography coupled selective reaction monitoring triple quadrupole mass spectrometry (LC-SRM/MS). Here, we present a detailed workflow including tips and we discuss advantages and disadvantages of using different types of MS for absolute quantification.
authors.affiliation: Department of Molecular Systems Biology, University of Vienna, Vienna, Austria.
authors.forename: David
authors.initials: D
authors.lastname: Lyon
authors.forename: Wolfram
authors.initials: W
authors.lastname: Weckwerth
authors.forename: Stefanie
authors.initials: S
authors.lastname: Wienkoop
mesh_terms: D002138:Calibration; D002853:Chromatography, Liquid / Q000295:instrumentation*; D007553:Isotope Labeling / Q000295:instrumentation*; D013058:Mass Spectrometry / Q000295:instrumentation*; D010455:Peptides / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*
chemical_list: D010455:Peptides; D011506:Proteins