title: The beneficial effect of encapsulated human adipose-derived stem cells in alginate hydrogel on neural differentiation.
abstract: Tissue engineering employs combination of biomaterials and cell therapy to develop new therapeutic strategies for neurodegenerative diseases, spinal cord, and traumatic brain injuries. Alginate is a biocompatible hydrogel, which has been used broadly to encapsulate many types of cells. Human adipose-derived stem cells (hADSCs) have appropriate property to differentiate into neuron-like cells. Therefore, the aim of this study was to evaluate the effect of alginate hydrogel on the viability and neural differentiation potential of induced hADSCs. After neural induction of isolated hADSCs and encapsulated in alginate hydrogel, the cell viability using MTT assay and their neural differentiation potential by immunocytochemical and real time RT-PCR analysis for neural markers (Nestin, GFAP, and MAP2) were evaluated. Expression of Nestin, GFAP, and MAP2 markers was significantly increased compare to monolayer induced cells (p<0.001), but we did not found any significant effect on viability of induced cells relative to monolayer induced cells. Although neural differentiation of encapsulated cells was increased relative to monolayer induced cells, the viability of these cells was not significantly different in alginate hydrogel as compared with monolayer induced cells.
authors.affiliation: Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, 81744-176, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Khosravizadeh
authors.forename: Shahnaz
authors.initials: S
authors.lastname: Razavi
authors.forename: Hamid
authors.initials: H
authors.lastname: Bahramian
authors.forename: Mohammad
authors.initials: M
authors.lastname: Kazemi
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D000464:Alginates; D018929:Cell Culture Techniques; D002470:Cell Survival; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis / Q000235:genetics; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006801:Humans; D020100:Hydrogels; D008855:Microscopy, Electron, Scanning; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics; D064231:Nestin / Q000032:analysis / Q000235:genetics; D055495:Neurogenesis; D061251:Primary Cell Culture; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000464:Alginates; D005904:Glial Fibrillary Acidic Protein; D006603:Hexuronic Acids; D020100:Hydrogels; C489788:MAP2 protein, human; D008869:Microtubule-Associated Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D020723:Glucuronic Acid
keywords: alginate hydrogel; human adipose-derived stem cells; neural differentiation; proliferation; tissue engineering