title: Extractive fermentation of gibberellic acid with free and immobilized Gibberella fujikuroi.
abstract: Gibberelic acid fermentation using extractive methods was carried out in the presence of corn oil and Alamine 336. Gibberella fujikuroi fungus (NRRL 2278) was used to produce gibberellic acid. Oleyl alcohol was a diluting agent for Alamine 336. The effects of oleyl alcohol (100%, v/v), corn oil (5-25%, v/v), the concentration of Alamine 336 in oleyl alcohol, and feeding air were examined in this study. According to the results, oleyl alcohol was not effective on the production. On the other hand, oleyl alcohol solutions containing 15-30% (v/v) Alamine 336 showed effects as a toxic substance. In order to reduce solvent toxicity, corn oil was used. Addition of corn oil increased the concentration of gibberellic acid 1.3-fold compared to the control. Then the effects of immobilization and co-immobilization on extractive gibberelic acid fermentation were investigated. The highest total gibberellic acid concentration of 158.9 mg/L was produced with immobilized cells and feeding air by using extractive fermentation. The yield of gibberellic acid increased about 2.6-fold compared with the shake-flask fermentation (60.5 mg/L) without organic solutions.
authors.affiliation: a Turkish Medicines and Medical Devices Agency , Environmental Reference Laboratory, Haymana Yolu , Ankara , Turkey.
authors.forename: Müge
authors.initials: M
authors.lastname: Gökdere
authors.forename: Selma
authors.initials: S
authors.lastname: Ateş
mesh_terms: D018914:Cells, Immobilized / Q000378:metabolism; D003314:Corn Oil / Q000494:pharmacology*; D005233:Fatty Alcohols / Q000494:pharmacology*; D005874:Gibberella / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism; D005875:Gibberellins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D005233:Fatty Alcohols; D005875:Gibberellins; C010268:oleyl alcohol; D003314:Corn Oil; C007842:gibberellic acid