title: Heat-inducible gene expression system by applying alternating magnetic field to magnetic nanoparticles.
abstract: By combining synthetic biology with nanotechnology, we demonstrate remote controlled gene expression using a magnetic field. Magnetite nanoparticles, which generate heat under an alternating magnetic field, have been developed to label cells. Magnetite nanoparticles and heat-induced therapeutic genes were introduced into tumor xenografts. The magnetically triggered gene expression resulted in tumor growth inhibition. This system shows great potential for controlling target gene expression in a space and time selective manner and may be used for remote control of cell functions via gene expression.
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Kyushu University , 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
authors.forename: Akihiko
authors.initials: A
authors.lastname: Ono
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kawabe
authors.forename: Masamichi
authors.initials: M
authors.lastname: Kamihira
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival; D015870:Gene Expression / Q000235:genetics / Q000528:radiation effects*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006358:Hot Temperature; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D050997:Nanomedicine / Q000379:methods*; D011401:Promoter Regions, Genetic / Q000235:genetics; D058615:Synthetic Biology / Q000379:methods*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: D018840:HSP70 Heat-Shock Proteins; C491129:HSPA7 protein, human; D008081:Liposomes; D058185:Magnetite Nanoparticles; D014409:Tumor Necrosis Factor-alpha