title: Effects of the dihydrolipoyl histidinate zinc complex against  carbon tetrachloride-induced hepatic fibrosis in rats.
abstract: OBJECTIVE
  This study investigated the effects of an antioxidant, dihydrolipoyl histidinate zinc complex (DHLHZn), on the hepatic fibrosis in the carbon tetrachloride (CCl4) rat model.
  
  
  METHODS
  The animals were divided into three groups: control, CCl4, and CCl4+DHLHZn. A histological assessment of the liver fibrosis was performed using stained liver samples. The oxidative stress and antioxidant levels were evaluated by measuring the malondialdehyde (MDA) and glutathione (GSH) levels in the liver. In addition, cultured human hepatic stellate cells (LI90) were exposed to antimycin-A (AMA) and divided into four groups: control, DHLHZn, AMA, and AMA+DHLHZn. The effects of DHLHZn on AMA-induced fibrosis were evaluated by measuring the expression of transforming growth factor (TGF)-β1 and collagen α1 (I).
  
  
  RESULTS
  The hepatic fibrosis in the CCl4+DHLHZn group was attenuated compared to that in the CCl4 group. The MDA levels in the CCl4+DHLHZn group were significantly lower than those of the CCl4 group, whereas the GSH levels in the CCl4+DHLHZn group were significantly higher than those of the CCl4 group. Furthermore, the relative mRNA expression of TGF-β1 and collagen α1 (I) in the AMA+DHLHZn group was significantly lower than that in the AMA group.
  
  
  CONCLUSIONS
  DHLHZn may attenuate the hepatic fibrosis induced by CCl4 by decreasing the degree of oxidative stress.
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Kawano
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ohta
authors.forename: Yukio
authors.initials: Y
authors.lastname: Iwashita
authors.forename: Yoko
authors.initials: Y
authors.lastname: Komori
authors.forename: Masafumi
authors.initials: M
authors.lastname: Inomata
authors.forename: Seigo
authors.initials: S
authors.lastname: Kitano
mesh_terms: D000818:Animals; D000968:Antimycin A; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D002251:Carbon Tetrachloride; D002478:Cells, Cultured; D005978:Glutathione / Q000378:metabolism; D055166:Hepatic Stellate Cells; D006639:Histidine / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D008099:Liver / Q000378:metabolism; D008103:Liver Cirrhosis / Q000139:chemically induced* / Q000188:drug therapy*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D017208:Rats, Wistar; D008063:Thioctic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D017967:Zinc Compounds / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D017967:Zinc Compounds; C557205:dihydrolipoylhistidine; D006639:Histidine; D008315:Malondialdehyde; D000968:Antimycin A; D008063:Thioctic Acid; D002251:Carbon Tetrachloride; D005978:Glutathione