title: Electroconvulsive seizure induces thrombospondin-1 in the adult rat hippocampus.
abstract: Synaptic dysfunction has recently gained attention for its involvement in mood disorders. Electroconvulsive therapy (ECT) possibly plays a role in synaptic repair. However, the underlying mechanisms remain uncertain. Thrombospondin-1 (TSP-1), a member of the TSP family, is reported to be secreted by astrocytes and to regulate synaptogenesis. We investigated the effects of electroconvulsive seizure (ECS) on the expression of TSPs in the adult rat hippocampus. Single and repeated ECS significantly increased TSP-1 mRNA expression after 2h and returned to sham levels at 24h. Conversely, the TSP-2 and -4 mRNA levels did not change. Only repeated ECS induced TSP-1 proteins. ECS also induced glial fibrillary acidic protein (GFAP) expression. The GFAP expression occurred later than the TSP-1 mRNA expression following single ECS; however, it occurred earlier and was more persistent following repeated ECS. ECS had no effect on the α2δ-1 or neuroligin-1 expressions, both of which are TSP-1 receptors. Furthermore, chronic treatment with antidepressants did not induce the expression of TSP-1 or GFAP. These findings suggest that repeated ECS, but not chronic treatment with antidepressants, induces TSP-1 expression partially via the activation of astrocytes. Therefore, TSP-1 is possibly involved in the synaptogenic effects of ECS.
authors.affiliation: Division of Psychiatry and Neuroscience, Institute for Clinical Research, National Hospital Organization (NHO) Kure Medical Center and Chugoku Cancer Center, 3-1 Aoyama, Kure, Japan.
authors.forename: Mami
authors.initials: M
authors.lastname: Okada-Tsuchioka
authors.forename: Masahiro
authors.initials: M
authors.lastname: Segawa
authors.forename: Naoto
authors.initials: N
authors.lastname: Kajitani
authors.forename: Kazue
authors.initials: K
authors.lastname: Hisaoka-Nakashima
authors.forename: Chiyo
authors.initials: C
authors.lastname: Shibasaki
authors.forename: Shigeru
authors.initials: S
authors.lastname: Morinobu
authors.forename: Minoru
authors.initials: M
authors.lastname: Takebayashi
mesh_terms: D051722:ADAM Proteins / Q000494:pharmacology; D000071097:ADAMTS1 Protein; D000704:Analysis of Variance; D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology; D003891:Desipramine / Q000494:pharmacology; D004195:Disease Models, Animal; D004597:Electroshock / Q000009:adverse effects*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects*; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D017374:Paroxetine / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000188:drug therapy / Q000209:etiology / Q000473:pathology*; D013917:Thrombin / Q000494:pharmacology; D019700:Thrombospondin 1 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000928:Antidepressive Agents; D005904:Glial Fibrillary Acidic Protein; D012333:RNA, Messenger; D019700:Thrombospondin 1; D017374:Paroxetine; D013917:Thrombin; D051722:ADAM Proteins; D000071097:ADAMTS1 Protein; C486324:Adamts1 protein, rat; D003891:Desipramine
keywords: ADAMTS-1; BCA; COMP; ECS; ECT; EGF; GAPDH; GFAP; Glia; MDD; Mood disorder; SDS; SPARC; Synaptogenesis; TSP; TSP Type 1 repeats; TSP-1; TSRs; a disintegrin and metalloproteinase with thrombospondin motifs 1; bicinchoninic acid; cartilage oligomeric matrix protein; electroconvulsive seizure; electroconvulsive therapy; epidermal growth factor; glial fibrillary acidic protein; glyceraldehyde-3-phosphate dehydrogenase; major depressive disorder; secreted protein acidic and rich in cysteine; sodium dodecyl sulfate; thrombospondin