title: Chondrogenic potential of stem cells derived from adipose tissue: a powerful pharmacological tool.
abstract: Chondrogenesis has been widely investigated in vitro using bone marrow-derived mesenchymal stromal cells (BM-MSCs) or primary chondrocytes. However, their use raises some issues partially circumvented by the availability of Adipose tissue-derived MSCs. Herein; we characterized the chondrogenic potential of human Multipotent Adipose-Derived Stem (hMADS) cells, and their potential use as pharmacological tool. hMADS cells are able to synthesize matrix proteins including COMP, Aggrecan and type II Collagen. Furthermore, hMADS cells express BMP receptors in a similar manner to BM-MSC, and BMP6 treatment of differentiated cells prevents expression of the hypertrophic marker type X Collagen. We tested whether IL-1β and nicotine could impact chondrocyte differentiation. As expected, IL-1β induced ADAMTS-4 gene expression and modulated negatively chondrogenesis while these effects were reverted in the presence of the IL-1 receptor antagonist. Nicotine, at concentrations similar to those observed in blood of smokers, exhibited a dose dependent increase of Aggrecan expression, suggesting an unexpected protective effect of the drug under these conditions. Therefore, hMADS cells represent a valuable tool for the analysis of in vitro chondrocyte differentiation and to screen for potentially interesting pharmacological drugs.
authors.affiliation: University Nice Sophia Antipolis, iBV, UMR 7277, 06100 Nice, France; CNRS, iBV, UMR 7277, 06100 Nice, France; Inserm, iBV, U1091, 06100 Nice, France; Service de Rhumatologie, Hospital l'Archet 1 CHU, 06200 Nice, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Roux
authors.forename: Didier F
authors.initials: DF
authors.lastname: Pisani
authors.forename: Hédi Ben
authors.initials: HB
authors.lastname: Yahia
authors.forename: Mansour
authors.initials: M
authors.lastname: Djedaini
authors.forename: Guillaume E
authors.initials: GE
authors.lastname: Beranger
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Chambard
authors.forename: Damien
authors.initials: D
authors.lastname: Ambrosetti
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Michiels
authors.forename: Véronique
authors.initials: V
authors.lastname: Breuil
authors.forename: Gérard
authors.initials: G
authors.lastname: Ailhaud
authors.forename: Liana
authors.initials: L
authors.lastname: Euller-Ziegler
authors.forename: Ez-Zoubir
authors.initials: EZ
authors.lastname: Amri
mesh_terms: D051722:ADAM Proteins / Q000235:genetics; D000071121:ADAMTS4 Protein; D000273:Adipose Tissue / Q000166:cytology*; D053674:Aggrecans / Q000096:biosynthesis; D055418:Bone Morphogenetic Protein 6 / Q000494:pharmacology; D052004:Bone Morphogenetic Protein Receptors / Q000378:metabolism; D002469:Cell Separation; D019902:Chondrocytes / Q000166:cytology*; D020219:Chondrogenesis / Q000235:genetics / Q000502:physiology*; D024121:Collagen Type X / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009538:Nicotine / Q000494:pharmacology; D011348:Procollagen N-Endopeptidase / Q000235:genetics
chemical_list: D053674:Aggrecans; D055418:Bone Morphogenetic Protein 6; D024121:Collagen Type X; D053583:Interleukin-1beta; D009538:Nicotine; D052004:Bone Morphogenetic Protein Receptors; D051722:ADAM Proteins; D011348:Procollagen N-Endopeptidase; D000071121:ADAMTS4 Protein; C000603918:ADAMTS4 protein, human
keywords: Chondrocyte; Differentiation; hBM-MSC; hMADS