title: [Effects of peroxisome proliferator-activated receptors γ on the expression of insulin receptor substrate-4 gene in rat cortical neurons and mouse brain].
abstract: OBJECTIVE
  To investigate the effect of peroxisome proliferator-activated receptors γ (PPARγ) on insulin receptor substrate-4 (IRS-4) gene expression in the brain.
  
  
  METHODS
  Primarily cultured cortical neurons from E17-18 Sprague Dawley rats, after 1 week of plating, were exposed to 10 µmol/L PPARγ agonist rosiglitazone for 0, 1, 4 or 24 h. Adult C57BL/6J mice or conditional brain PPARγ knock-out mice (B-PPARγ-KO, BKO) received an intraperitoneal injection of rosiglitazone in 10% DMSO at 12 mg/kg or injection of the same volume of saline containing 10% DMSO. The effect of rosiglitazone on the survival of the neurons was examined by MTT assay. The expression of IRS-4 mRNA was analyzed by real-time quantitative PCR.
  
  
  RESULTS
  The survival of the cortical neurons showed no significant difference between the agonist groups and the control group. The expression of IRS-4 mRNA was significantly up-regulated in the cortical tissues and neurons of the agonist groups compared with the control groups (P<0.05), but in BKO mice without treatment, IRS-4 mRNA expression was significantly down-regulated (P<0.05).
  
  
  CONCLUSIONS
  PPARγ can enhance the expression of IRS-4 mRNA in the brain.
authors.affiliation: Department of Neurobiology, Southern Medical University, Guangzhou 510515, China. E-mail: minqing_tang@163.com.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhang
authors.forename: Siying
authors.initials: S
authors.lastname: Meng
authors.forename: Lifang
authors.initials: L
authors.lastname: Lin
authors.forename: Qiaoqi
authors.initials: Q
authors.lastname: Wu
authors.forename: Riyang
authors.initials: R
authors.lastname: Zhou
authors.forename: Xuemin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D005260:Female; D018014:Gene Transfer Techniques; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D047495:PPAR gamma / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: C491532:IRS4 protein, rat; D055504:Insulin Receptor Substrate Proteins; C491531:Irs4 protein, mouse; D047495:PPAR gamma; D012333:RNA, Messenger; D045162:Thiazolidinediones; D000077154:Rosiglitazone