title: Antifungal activity of Microgramma vacciniifolia rhizome lectin on genetically distinct Fusarium oxysporum f. sp. lycopersici races.
abstract: Fusarium oxysporum f. sp. lycopersici races 1, 2, and 3 deteriorate tomato crops since they cause a vascular wilt. Lectins are carbohydrate-binding proteins with hemagglutinating and antifungal activities. This work reports that Microgramma vacciniifolia rhizome lectin (MvRL) inhibits F. oxysporum f. sp. lycopersici race 3 growth (61 %) more intensely than of races 1 (55 %) and 2 (45 %). The hemagglutinating activity of MvRL was inhibited by glycoprotein preparations from mycelia of races 1, 2, and 3, and these data indicate that lectin carbohydrate-binding sites recognized glycosylated molecules from races. Inter-simple sequence repeat (ISSR) marker system showed that race 3 is genetically distinct from races 1 and 2, and thus the highest sensitiveness of F. oxysporum f. sp. lycopersici race 3 to MvRL may be due to molecular characteristics of this race.
authors.affiliation: Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-420, Brazil.
authors.forename: Lidiane Pereira de
authors.initials: LP
authors.lastname: Albuquerque
authors.forename: Giselly Maria de Sá
authors.initials: GM
authors.lastname: Santana
authors.forename: Thiago Henrique
authors.initials: TH
authors.lastname: Napoleão
authors.forename: Luana Cassandra Breitenbach Barroso
authors.initials: LC
authors.lastname: Coelho
authors.forename: Márcia Vanusa da
authors.initials: MV
authors.lastname: Silva
authors.forename: Patrícia Maria Guedes
authors.initials: PM
authors.lastname: Paiva
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D029624:Ferns / Q000737:chemistry*; D005656:Fungal Proteins / Q000378:metabolism; D005670:Fusarium / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D014644:Genetic Variation; D008826:Microbial Sensitivity Tests; D037121:Plant Lectins / Q000494:pharmacology*; D027343:Rhizome / Q000737:chemistry*
chemical_list: D000935:Antifungal Agents; D005656:Fungal Proteins; D037121:Plant Lectins