title: The post-thaw irradiation of avian spermatozoa with He-Ne laser differently affects chicken, pheasant and turkey sperm quality.
abstract: The effects of post-thaw Helium-Neon (He-Ne) laser irradiation on mobility and functional integrity of frozen/thawed chicken, pheasant and turkey spermatozoa were investigated. Cytochrome C oxidase (COX) activity was also determined as a measure of the effect of irradiation on mitochondrial bioenergetics. Semen samples from each species were collected, processed and frozen according to the pellet procedure. After thawing, each semen sample was divided into two subsamples: the first one was the control; the second one was irradiated with a single mode continuous He-Ne laser wave (wavelength 632.8 nm; 6 mW; 3.96 J/cm(2)). Then the samples were assessed for sperm mobility (Accudenz(®) swim-down test), viability (SYBR-14/PI staining), osmotic-resistance (HOS test) and COX activity. The irradiation was effective P<0.05 increasing sperm motility in the turkey semen (0.228 ± 0.01 compared with 0.294 ± 0.02). The irradiation also caused an increase (P<0.05) of the COX activity in pheasant (+135 ± 4%) and turkey (+116 ± 4%) sperm, without affecting viability and osmotic-resistance. The COX was positively correlated (P<0.05) with the viability of chicken sperm, however no significant interactions were found between mobility and COX activity in the three avian species. Due to the difference in energetic metabolism among avian species used in this study, the He-Ne laser irradiation has a differential action on bio-stimulation of turkey, chicken and pheasant spermatozoa. The present results are the first to elucidate the possibility for restoration of motility of cryopreserved avian spermatozoa by bio-stimulation provided via He-Ne laser irradiation.
authors.affiliation: Department of Agricultural, Environmental and Food Sciences, University of Molise, via De Sanctis, 86100 Campobasso, Italy. Electronic address: nicolaia@unimol.it.
authors.forename: N
authors.initials: N
authors.lastname: Iaffaldano
authors.forename: G
authors.initials: G
authors.lastname: Paventi
authors.forename: R
authors.initials: R
authors.lastname: Pizzuto
authors.forename: S
authors.initials: S
authors.lastname: Passarella
authors.forename: S
authors.initials: S
authors.lastname: Cerolini
authors.forename: L
authors.initials: L
authors.lastname: Zaniboni
authors.forename: M
authors.initials: M
authors.lastname: Marzoni
authors.forename: A
authors.initials: A
authors.lastname: Castillo
authors.forename: M P
authors.initials: MP
authors.lastname: Rosato
mesh_terms: D000818:Animals; D002645:Chickens; D015925:Cryopreservation / Q000379:methods; D005615:Freezing; D046094:Galliformes / Q000378:metabolism; D054020:Lasers, Gas / Q000009:adverse effects*; D008297:Male; D055101:Semen Analysis / Q000662:veterinary*; D012662:Semen Preservation / Q000009:adverse effects* / Q000379:methods; D013094:Spermatozoa / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects*; D014422:Turkeys
keywords: Avian species; Cryopreservation; Laser helium–neon; Mitochondrial function; Spermatozoa