title: Topical application of recombinant platelet-derived growth factor increases the rate of healing and the level of proteins that regulate this response.
abstract: A bipedicle ischaemic rat skin flap model was used to study the effects of daily topical applications of platelet-derived growth factor (PDGF) on the healing of ischaemic wounds. Levels of tumour necrosis factor-alpha (TNFA), interleukin 1-beta (IL1B) and both the latent and active forms of matrix metalloproteinase 2 (MMP2) and 9 (MMP9) were measured. Full-thickness wounds were made on a total of 72 adult male Sprague-Dawley rats. Each group of 18 rats with normal and ischaemic wounds received either vehicle or 0·01% recombinant PDGF-BB. Additional applications were made on the wounds on a daily basis. Wound areas were measured at 0, 1, 3, 5, 7 9 and 13 days after wounding. Ischaemia caused a delay in wound healing as well as an increase in TNFA, IL1B and both the pro and active forms of MMP2 and MMP9. PDGF accelerated the rate of wound healing in both normal and ischaemic wounds and negated the effect of ischaemia. PDGF reduced the TNFA concentration in both normal and ischaemic wounds, and the rate of wound healing closely resembled the pattern of TNFA protein expression. PDGF also reduced both the magnitude and duration of the increases in IL1B and both the pro and active forms of MMP2 and MMP9 induced by ischaemia.
authors.affiliation: Marquette General Hematology Oncology, Escanaba, MI, USA.
authors.forename: Santosh
authors.initials: S
authors.lastname: Gowda
authors.affiliation: Department of Dermatology, New York Medical College, New York, NY, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Weinstein
authors.affiliation: Department of Ophthalmology, University of Florida, Gainesville, FL, USA.|University of Florida's Institute for Wound Healing, Gainesville, FL, USA.
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Blalock
authors.affiliation: University of Maryland School of Medicine, Baltimore, MD, USA.
authors.forename: Kavita
authors.initials: K
authors.lastname: Gandhi
authors.affiliation: University of Florida's Institute for Wound Healing, Gainesville, FL, USA.|Department of Surgery, Division of Plastic and Reconstructive Surgery, University of Florida, Gainesville, FL, USA.
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Mast
authors.affiliation: Southeastern Hand Center, Ocala, FL, USA.
authors.forename: Gloria
authors.initials: G
authors.lastname: Chin
authors.affiliation: Department of Ophthalmology, University of Florida, Gainesville, FL, USA.|University of Florida's Institute for Wound Healing, Gainesville, FL, USA.
authors.forename: Gregory S
authors.initials: GS
authors.lastname: Schultz
mesh_terms: D000287:Administration, Topical; D043925:Angiogenesis Inducing Agents / Q000627:therapeutic use*; D000818:Animals; D000077214:Becaplermin; D004195:Disease Models, Animal; D053583:Interleukin-1beta / Q000378:metabolism; D007511:Ischemia / Q000150:complications / Q000378:metabolism; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D020574:Proto-Oncogene Proteins c-sis / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology*; D014950:Wounds, Penetrating / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism
chemical_list: D043925:Angiogenesis Inducing Agents; D053583:Interleukin-1beta; D020574:Proto-Oncogene Proteins c-sis; D014409:Tumor Necrosis Factor-alpha; D000077214:Becaplermin; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9
keywords: Matrix metalloproteinases; Platelet-derived growth factor; Rat ischaemic skin model; Tumour necrosis factor-alpha; Wound healing