title: p35 promotes the differentiation of amacrine cell subtype in the zebrafish retina under the regulation of egr1.
abstract: BACKGROUND
  Early growth response 1 (egr1) is a transcription factor (TF) for controlling the differentiation of Parvalbumin (Parv) -expressing amacrine cells (ACs) in zebrafish. However, the downstream factors of this process have not been identified. The purpose of this study was to investigate the role of p35, a neuronal-specific activator of cyclin-dependent kinase 5 (Cdk5) and a known in vitro target of egr1, in the differentiation of these ACs.
  
  
  RESULTS
  In the p35-knockdown retinas, Parv+ but not islet1+ ACs were specifically reduced. This phenotype was highly similar to that in the Egr1-knockdown retinas. Furthermore, p35 expression was reduced in the Egr1-knockdown retinas, particularly in the AC region; while egr1 was only modestly reduced in this region in the p35-knockdown retinas.
  
  
  CONCLUSIONS
  p35 likely acts downstream of egr1 to control the differentiation of Parv+ ACs.
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, Indiana.
authors.forename: Liyun
authors.initials: L
authors.lastname: Zhang
authors.forename: Sylvia
authors.initials: S
authors.lastname: Bonilla
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yuk Fai
authors.initials: YF
authors.lastname: Leung
mesh_terms: D025042:Amacrine Cells / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D051766:Early Growth Response Protein 1 / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D055785:Gene Knockdown Techniques; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008856:Microscopy, Fluorescence; D060172:Morpholinos / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010320:Parvalbumins / Q000378:metabolism; D012160:Retina / Q000196:embryology* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015027:Zebrafish / Q000196:embryology*
chemical_list: D017931:DNA Primers; D051766:Early Growth Response Protein 1; D060172:Morpholinos; D009419:Nerve Tissue Proteins; D010320:Parvalbumins; C089495:neuronal Cdk5 activator (p25-p35)
keywords: amacrine cells; cdk5; cdk5r1b; egr1; p35; retinal development; smarca4; zebrafish