title: Profiling polyphenols in five Brassica species microgreens by UHPLC-PDA-ESI/HRMS(n.).
abstract: Brassica vegetables are known to contain relatively high concentrations of bioactive compounds associated with human health. A comprehensive profiling of polyphenols from five Brassica species microgreens was conducted using ultrahigh-performance liquid chromatography photodiode array high-resolution multistage mass spectrometry (UHPLC-PDA-ESI/HRMS(n)). A total of 164 polyphenols including 30 anthocyanins, 105 flavonol glycosides, and 29 hydroxycinnamic acid and hydroxybenzoic acid derivatives were putatively identified.The putative identifications were based on UHPLC-HRMS(n) analysis using retention times, elution orders, UV-vis and high-resolution mass spectra, and an in-house polyphenol database as well as literature comparisons. This study showed that these five Brassica species microgreens could be considered as good sources of food polyphenols.
authors.affiliation: Food Composition and Methods Development Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture , 10300 Baltimore Avenue, Beltsville, Maryland 20705, United States.
authors.forename: Jianghao
authors.initials: J
authors.lastname: Sun
authors.forename: Zhenlei
authors.initials: Z
authors.lastname: Xiao
authors.forename: Long-Ze
authors.initials: LZ
authors.lastname: Lin
authors.forename: Gene E
authors.initials: GE
authors.lastname: Lester
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.forename: James M
authors.initials: JM
authors.lastname: Harnly
authors.forename: Pei
authors.initials: P
authors.lastname: Chen
mesh_terms: D001937:Brassica / Q000737:chemistry* / Q000145:classification / Q000254:growth & development; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods; D014675:Vegetables / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D059808:Polyphenols