title: Antiviral and antiproliferative effects of canine interferon-λ1.
abstract: Interferon (IFN)-λs, members of the type III IFN group, were recently identified in several vertebrates. Although IFN-λs have the potential to be utilized as antiviral and antitumor agents in veterinary medicine, the biological properties of IFN-λs have not yet been studied in companion animals. In this study, we analyzed the expression of canine IFN-λs and their receptors, produced a recombinant canine IFN-λ1 protein, and investigated its antiviral and antiproliferative activities using a canine kidney epithelial cell line, MDCK cells. MDCK cells were found to express type III IFN molecules, IFN-λ1 and IFN-λ3, and the receptors, IFNλR1 and IL10R2. IFN-λ1 was induced faster than IFN-λ3 by stimulation with poly (I:C). His-tagged IFN-λ1 protein expressed in Escherichia coli inhibited cytolytic plaque formation by influenza A virus infection, and induced the expression of interferon-stimulated genes, Mx1 and OAS1, in MDCK cells. In addition, recombinant IFN-λ1 inhibited the proliferation of MDCK cells slightly. These effects were observed in a dose-dependent manner. These results indicate that canine IFN-λ1 has antiviral effect, and suggest the potential applicability of canine IFN-λ1 as a therapeutic agent.
authors.affiliation: Laboratory of Veterinary Biochemistry, Tottori University, Tottori 680-8553, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Ichihashi
authors.forename: Atsushi
authors.initials: A
authors.lastname: Asano
authors.forename: Tatsufumi
authors.initials: T
authors.lastname: Usui
authors.forename: Takashi
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Yamano
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004285:Dogs; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000494:pharmacology*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D000998:Antiviral Agents; D011994:Recombinant Proteins; D007371:Interferon-gamma
keywords: Antiproliferation; Antiviral activity; Dog; IFN-λ1; Interferon-stimulated genes