title: Trehalose and sorbitol alter the kinetic pattern of inactivation of glutamate dehydrogenase during drying in levitated microdroplets.
abstract: A single-droplet acoustic levitator was used to determine the drying rate and the kinetics of inactivation of glutamate dehydrogenase in the presence of added trehalose or sorbitol. The solution was also spray dried under the same process condition of drying gas temperature on a bench-top machine. Both trehalose and sorbitol delay the point of onset of enzyme inactivation which lies after the critical point of drying. Both carbohydrates also reduce the apparent rate constant of inactivation calculated during the subsequent inactivation phase. The carbohydrates stabilise, therefore, the enzyme during droplet drying and particle formation mainly during the falling rate drying period. There is no difference between the stabilising effects of the two carbohydrates when examined as levitated single droplets. This suggests the importance of water replacement as a stabilising mechanism in the levitated droplets/particles. On spray drying, the trehalose stabilises the enzyme better than does the sorbitol at a drying gas (outlet) temperature of 60°C. This suggests glass formation with the trehalose but not the sorbitol during the very rapid drying process of small-atomised droplets in the spray dryer.
authors.affiliation: Division of Pharmaceutics, Friedrich-Alexander-University, Erlangen, Germany.
authors.forename: Elke
authors.initials: E
authors.lastname: Lorenzen
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Lee
mesh_terms: D000818:Animals; D002417:Cattle; D003890:Desiccation / Q000379:methods; D004789:Enzyme Activation; D004795:Enzyme Stability; D005969:Glutamate Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D010316:Particle Size; D044367:Phase Transition; D013012:Sorbitol / Q000737:chemistry*; D014199:Trehalose / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D013012:Sorbitol; D014199:Trehalose; D005969:Glutamate Dehydrogenase
keywords: drying; enzyme; glass transition; levitator; protein; spray-drying; stabilization