title: Cell type-dependent effects of corticosteroid on periostin production by primary human tissue cells.
abstract: Overproduction of periostin, an IL-13-inducible matricellular protein, despite corticosteroid treatment is thought to be involved in the chronicity of allergic inflammation seen in corticosteroid-refractory tissue fibrosis. Therefore, we hypothesized that some tissue cells must produce periostin in a corticosteroid-insensitive manner. Here, we show that IL-4 and IL-13 each induced comparable levels of periostin production by primary normal human fibroblasts and microvascular endothelial cells derived from lung and skin. Dexamethasone, a corticosteroid, completely inhibited IL-4/13-induced, but did not affect TGF-β-induced, periostin production by fibroblasts. In contrast, dexamethasone synergistically enhanced IL-4/13-induced periostin production by microvascular endothelial cells. TGF-β did not induce periostin production by microvascular endothelial cells. Our novel findings suggest that IL-4/13-induced microvascular endothelium-derived and/or TGF-β-induced fibroblast-derived periostin might play a pivotal role in corticosteroid-refractory tissue fibrosis, leading to chronic allergic inflammation in the lung and/or skin.
authors.affiliation: Department of Health and Psychosocial Medicine, Aichi Medical University School of Medicine, Aichi; Department of Allergy and Immunology, National Research Institute for Child Health and Development, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shoda
authors.forename: K
authors.initials: K
authors.lastname: Futamura
authors.forename: F
authors.initials: F
authors.lastname: Kobayashi
authors.forename: H
authors.initials: H
authors.lastname: Saito
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
authors.forename: A
authors.initials: A
authors.lastname: Matsuda
mesh_terms: D015815:Cell Adhesion Molecules / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D002474:Cell-Free System; D003907:Dexamethasone / Q000494:pharmacology*; D005347:Fibroblasts / Q000276:immunology / Q000473:pathology; D005355:Fibrosis / Q000276:immunology; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D018836:Inflammation Mediators / Q000502:physiology; D018793:Interleukin-13 / Q000502:physiology; D015847:Interleukin-4 / Q000502:physiology; D008168:Lung / Q000276:immunology / Q000473:pathology; D012867:Skin / Q000276:immunology / Q000473:pathology; D014018:Tissue Distribution / Q000276:immunology; D053773:Transforming Growth Factor beta1 / Q000502:physiology
chemical_list: D015815:Cell Adhesion Molecules; C508598:IL4 protein, human; D018836:Inflammation Mediators; D018793:Interleukin-13; C512835:POSTN protein, human; D053773:Transforming Growth Factor beta1; D015847:Interleukin-4; D003907:Dexamethasone
keywords: IL-13; corticosteroid; fibroblasts; microvascular endothelial cells; periostin