title: [Neurogenesis in the dentate gyrus of the hippocampus in a rat model of intracerebral hemorrhage].
abstract: OBJECTIVE
  To investigate whether intracerebral hemorrhage (ICH) can promote neurogenesis in the dentate gyrus (DG) of rat hippocampus.
  
  
  METHODS
  Western blot analysis, immunohistochemical staining, and immunofluorescent double labeling combined with confocal microscope were used to detect neurogenesis in the DG of the hippocampus in rats after ICH.
  
  
  RESULTS
  The expression of DCX protein in the ipsilateral DG of the hippocampus was enhanced in the rats 1 day after ICH (0.202∓0.062) as compared with that in normal rats (0.127∓0.088), reaching the peak level at 14 days (0.771∓0.108, P<0.01) and beginning to decrease at 28 days (0.582∓0.121, P<0.01). Meanwhile, DCX-positive cells and BrdU-positive cells, and DCX/BrdU double-labeled cells were detected in the DG of the hippocampus. Compared with those in the control group, BrdU/NeuN double-labeled cells were markedly increased in the granular cell layer of the DG at 28 days after ICH (1.808∓1.020 vs 5.654∓1.671, P<0.01).
  
  
  CONCLUSIONS
  ICH can promote neurogenesis in the DG of rat hippocampus.
authors.affiliation: Department of Human Anatomy, Xi'an Medical University, Xi'an 710021, China. E-mail: xmux@qq.com.
authors.forename: Xi
authors.initials: X
authors.lastname: Xu
authors.forename: Jianyun
authors.initials: J
authors.lastname: Zheng
authors.forename: Junfeng
authors.initials: J
authors.lastname: Zhang
authors.forename: Xinlin
authors.initials: X
authors.lastname: Chne
authors.forename: PengBo
authors.initials: P
authors.lastname: Yang
authors.forename: Jianxin
authors.initials: J
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000378:metabolism; D002543:Cerebral Hemorrhage / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D018891:Dentate Gyrus / Q000378:metabolism* / Q000503:physiopathology; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D006624:Hippocampus / Q000378:metabolism* / Q000503:physiopathology; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D009479:Neuropeptides / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D034961:Antigens, Nuclear; C000714328:DCX protein, human; C000714848:Dcx protein, rat; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; C546339:Rbfox3 protein, rat; D001973:Bromodeoxyuridine