title: A Uchl1-Histone2BmCherry:GFP-gpi BAC transgene for imaging neuronal progenitors.
abstract: Uchl1 encodes the protein gene product 9.5 antigen (PGP9.5) that is a widely used to identify migrating neural progenitors in the PNS, mature neurons of the central and peripheral nervous systems, as well as neuroendocrine cells. To facilitate analysis of developing peripheral neurons, we linked regulatory regions of Uchl1 carried within a 160kb bacterial artificial chromosome (BAC) to the dual fluorescent reporter H2BmCherry:GFP-gpi. The Uchl1-H2BmCherry:GFP-gpi transgene exhibits robust expression and allows clear discrimination of individual cells and cellular processes in cranial ganglia, sympathetic chain, the enteric nervous system (ENS), and autonomic ganglia of the urogenital system. The transgene also labels subsets of cells in endocrine tissues where earlier in situ hybridization (ISH) studies have previously identified expression of this deubiquinating enzyme. The Uchl1-H2BmCherry:GFP-gpi transgene will be a powerful tool for static and live imaging, as well as isolation of viable neural progenitors to investigate processes of autonomic neurogenesis.
authors.affiliation: Division of Genetic Medicine, Department of Medicine, Vanderbilt University School of Medicine, 529 Light Hall, 2215 Garland Avenue, Nashville, Tennessee.
authors.forename: Carrie B
authors.initials: CB
authors.lastname: Wiese
authors.forename: Nicole
authors.initials: N
authors.lastname: Fleming
authors.forename: Dennis P
authors.initials: DP
authors.lastname: Buehler
authors.forename: E Michelle
authors.initials: EM
authors.lastname: Southard-Smith
mesh_terms: D000818:Animals; D002465:Cell Movement; D022202:Chromosomes, Artificial, Bacterial; D017615:Enteric Nervous System / Q000502:physiology; D005434:Flow Cytometry; D005724:Ganglia / Q000378:metabolism; D017930:Genes, Reporter; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D019076:Transgenes; D043222:Ubiquitin Thiolesterase / Q000235:genetics* / Q000378:metabolism*
chemical_list: D006657:Histones; D011993:Recombinant Fusion Proteins; D043222:Ubiquitin Thiolesterase; C496976:Uchl1 protein, mouse
keywords: PGP9.5; Uchl1; bacterial artificial chromosome (BAC); enteric nervous system; lower urinary tract; neural crest; neural progenitor; neurogenesis; oncogenesis