title: Comparative proteomic analysis of the hepatic response to heat stress in Muscovy and Pekin ducks: insight into thermal tolerance related to energy metabolism.
abstract: The Pekin duck, bred from the mallard (Anas platyrhynchos) in china, is one of the most famous meat duck species in the world. However, it is more sensitive to heat stress than Muscovy duck, which is believed to have originated in South America. With temperature raising, mortality, laying performance, and meat quality of the Pekin duck are severely affected. This study aims to uncover the temperature-dependent proteins of two duck species using comparative proteomic approach. Duck was cultured under 39°C ± 0.5°C for 1 h, and then immediately returned to 20°C for a 3 h recovery period, the liver proteins were extracted and electrophoresed in two-dimensional mode. After analysis of gel images, 61 differentially expressed proteins were detected, 54 were clearly identified by MALDI TOF/TOF MS. Of the 54 differentially expressed protein spots identified, 7 were found in both species, whereas 47 were species specific (25 in Muscovy duck and 22 in Pekin duck). As is well known, chaperone proteins, such as heat shock protein (HSP) 70 and HSP10, were abundantly up-regulated in both species in response to heat stress. However, we also found that several proteins, such as α-enolase, and S-adenosylmethionine synthetase, showed different expression patterns in the 2 duck species. The enriched biological processes were grouped into 3 main categories according to gene ontology analysis: cell death and apoptosis (20.93%), amino acid metabolism (13.95%) and oxidation reduction (20.93%). The mRNA levels of several differentially expressed protein were investigated by real-time RT-PCR. To our knowledge, this study is the first to provide insights into the differential expression of proteins following heat stress in ducks and enables better understanding of possible heat stress response mechanisms in animals.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, People's Republic of China ; Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zeng
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Jiang
authors.forename: Jinjun
authors.initials: J
authors.lastname: Li
authors.forename: Deqian
authors.initials: D
authors.lastname: Wang
authors.forename: Guoqin
authors.initials: G
authors.lastname: Li
authors.forename: Lizhi
authors.initials: L
authors.lastname: Lu
authors.forename: Genlin
authors.initials: G
authors.lastname: Wang
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics / Q000502:physiology; D000818:Animals; D018835:Chaperonin 10 / Q000235:genetics / Q000378:metabolism; D004372:Ducks / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004734:Energy Metabolism / Q000235:genetics / Q000502:physiology; D020869:Gene Expression Profiling / Q000379:methods; D063990:Gene Ontology; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006358:Hot Temperature; D008099:Liver / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation
chemical_list: D018835:Chaperonin 10; D018840:HSP70 Heat-Shock Proteins; D020543:Proteome