title: Simultaneous ligand fishing and identification of human serum albumin binders from Eucommia ulmoides bark using surface plasmon resonance-high performance liquid chromatography-tandem mass spectrometry.
abstract: Eucommia ulmoides bark has long been known as tonic herb, however, the systematic biochemical fingerprint analysis and corresponding structural identification are inadequate so far. Here we describe on-line surface plasmon resonance-high performance liquid chromatography-tandem mass spectrometry (SPR-HPLC-MS/MS) to fish out and identify human serum albumin (HSA) binders from E. ulmoides bark. A total of 22 HSA binders, including 4 iridoids, 11 lignans, 3 flavonoids and 4 phenolic acids, were identified or tentatively characterized based on their retention times, UV spectra and MS/MS spectrum with references or literature data, among which, geniposidic acid, geniposide and chlorogenic acid were the predominant HSA binders. In addition, the major characteristic fragmentation pathways of iridoids and lignans were investigated. The higher reusability and stability of the immobilized HSA allow the method cost-effective and high-throughput. Compared with conventional HPLC-MS/MS for chemical fingerprint analysis, SPR-HPLC-MS/MS is simple and easy to provide biochemical fingerprint, moreover, it is easy to identify low-content HSA binders because of the sample clean-up/concentration procedure.
authors.affiliation: Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, China.
authors.forename: Mijun
authors.initials: M
authors.lastname: Peng
authors.forename: Yuping
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shuyun
authors.initials: S
authors.lastname: Shi
authors.forename: Sheng
authors.initials: S
authors.lastname: Peng
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D031283:Eucommiaceae / Q000737:chemistry*; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism*; D039823:Iridoids / Q000737:chemistry; D008024:Ligands; D010636:Phenols / Q000737:chemistry; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D020349:Surface Plasmon Resonance / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D055767:Immobilized Proteins; D039823:Iridoids; D008024:Ligands; D010636:Phenols; D010936:Plant Extracts; D012709:Serum Albumin
keywords: Eucommia ulmoides bark; HPLC–MS/MS; HSA; Iridoid; Lignan; SPR