title: [Optimization the formulation of Eisemia foetida protein burn spray by response surface methodology].
abstract: OBJECTIVE
  To optimize the formulation of Eisemia foetida protein (EFP) burn spray.
  
  
  METHODS
  A five-factor, three-level response surface method was employed; The response variable was the proliferation effect of EFP on NIH3T3 cells.
  
  
  RESULTS
  The optimization formulation was as follows: the proportion of EFP, glycerol and mannitol was 0.91%, 1.42% and 5%, respectively; 0.02 mol/L Na2 HPO4 and 0.01 mol/L citric acid buffer system corresponding pH value was 7.0.
  
  
  CONCLUSIONS
  The response surface method is reliable, efficient and suitable for optimizing the formulation of EFP burn spray.
authors.affiliation: Weihai International Biotechnology R&D Center, Shangdong University, Weihai 264209, China.
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Hou
authors.forename: Shu-Liang
authors.initials: SL
authors.lastname: Song
authors.forename: Hao
authors.initials: H
authors.lastname: Liang
authors.forename: Wen-Xin
authors.initials: WX
authors.lastname: Huang
authors.forename: Ai-Guo
authors.initials: AG
authors.lastname: Ji
mesh_terms: D000336:Aerosols; D000818:Animals; D002056:Burns / Q000188:drug therapy; D049109:Cell Proliferation / Q000187:drug effects*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D019343:Citric Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008353:Mannitol / Q000737:chemistry*; D008419:Materia Medica / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D041681:NIH 3T3 Cells; D009835:Oligochaeta / Q000737:chemistry*; D011310:Preservatives, Pharmaceutical / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*
chemical_list: D000336:Aerosols; D008419:Materia Medica; D011310:Preservatives, Pharmaceutical; D011506:Proteins; D019343:Citric Acid; D008353:Mannitol