title: Evaluation of the antibacterial activity of Piperaceae extracts and nisin on Alicyclobacillus acidoterrestris.
abstract: Alicyclobacillus acidoterrestris is a gram-positive aerobic bacterium. This bacterium resists pasteurization temperatures and low pH and is usually involved in the spoilage of juices and acidic drinks. The objective of this study was to evaluate the antibacterial activities of nisin and the species Piper (Piperaceae) on A. acidoterrestris. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined by the broth microdilution method. The species Piper aduncum had the lowest MIC and an MBC of 15.6 μg/mL and was selected for fractionation. Six fractions were obtained, and the dichloromethane fraction (F.3) had the lowest MIC/MBC (7.81 μg/mL). The dichloromethane fraction was again fractionized, and a spectral analysis revealed that the compound was prenylated chromene (F.3.7). The checkerboard method demonstrated that the crude extract (CE) of P. aduncum plus nisin had a synergistic interaction (fractional inhibitory concentration [FIC] = 0.24). The bactericidal activity of (F.3.7) was confirmed by the time-kill curve. P. aduncum, nisin, and prenylated chromene exhibited strong antibacterial activity against the spores and vegetative cells of A. acidoterrestris. The results of this study suggest that extracts of the genus Piper may provide an alternative to the use of thermal processing for controlling A. spoilage.
authors.affiliation: Programa de Pós-Graduação em Ciência de Alimentos, Univ, Estadual de Maringá, Maringá, Brazil.
authors.forename: Suelen P
authors.initials: SP
authors.lastname: Ruiz
authors.forename: Márcia Maria Dos
authors.initials: MM
authors.lastname: Anjos
authors.forename: Vanessa S
authors.initials: VS
authors.lastname: Carrara
authors.forename: Juliana N
authors.initials: JN
authors.lastname: Delima
authors.forename: Diógenes Aparício G
authors.initials: DA
authors.lastname: Cortez
authors.forename: Tânia U
authors.initials: TU
authors.lastname: Nakamura
authors.forename: Celso V
authors.initials: CV
authors.lastname: Nakamura
authors.forename: Benício A
authors.initials: BA
authors.lastname: de Abreu Filho
mesh_terms: D056450:Alicyclobacillus / Q000187:drug effects* / Q000254:growth & development; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D004305:Dose-Response Relationship, Drug; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control*; D005516:Food Microbiology; D005519:Food Preservation; D006863:Hydrogen-Ion Concentration; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D009561:Nisin / Q000032:analysis / Q000494:pharmacology*; D061352:Pasteurization; D026321:Piperaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D013696:Temperature; D014709:Vero Cells
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D009561:Nisin; C102526:nisin A
keywords: alicyclobacillus acidoterrestris; nisin; piperaceae; prenylated chromene; synergism