title: The effect of bacterial dose and foal age at challenge on Rhodococcus equi infection.
abstract: While Rhodococcus equi remains the most common cause of subacute or chronic granulomatous bronchopneumonia in foals, development of a relevant model to study R. equi infection has proven difficult. The objective of this study was to identify a challenge dose of R. equi that resulted in slow progressive disease, spontaneous regression of lung lesions and age-dependent susceptibility. Foals less than one-week of age were challenged intratracheally using either 10(6), 10(5), 10(4), 10(3) or 10(2) cfu of R. equi. Two doses (10(3) cfu and 10(5) cfu) were used to challenge 2 and 3-week-old, and 3 and 6-week-old foals, respectively. Physical examination, thoracic ultrasound and blood work were performed. Foals were euthanized at the end of the study or when clinical signs of pneumonia developed. All foals were necropsied and their lung lesions scored. Foals challenged with low concentrations of R. equi developed slow progressive pneumonia and approximately 50% of the foals recovered spontaneously. Likewise, macroscopic (>1cm diameter) pyogranulomatous lesions were only observed when low doses of R. equi were used. Clinical pneumonia was not seen after low dose challenge in the 3-week-old foals or in the 6-week-old foals. This study demonstrates that the use of low doses of R. equi to challenge neonatal foals provides an improved model for studying this disease. Furthermore, susceptibility to R. equi infection was shown to diminish early in the foal's life, as has been reported in the field.
authors.affiliation: Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY 40546-0099, United States.
authors.forename: M
authors.initials: M
authors.lastname: Sanz
authors.forename: A
authors.initials: A
authors.lastname: Loynachan
authors.forename: L
authors.initials: L
authors.lastname: Sun
authors.forename: A
authors.initials: A
authors.lastname: Oliveira
authors.forename: P
authors.initials: P
authors.lastname: Breheny
authors.forename: D W
authors.initials: DW
authors.lastname: Horohov
mesh_terms: D000193:Actinomycetales Infections / Q000150:complications / Q000401:mortality / Q000662:veterinary*; D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D058491:Bacterial Load; D004906:Erythrocyte Count; D006454:Hemoglobins / Q000032:analysis; D006734:Horse Diseases / Q000000981:diagnostic imaging / Q000382:microbiology* / Q000401:mortality / Q000473:pathology; D006736:Horses; D008214:Lymphocytes; D011014:Pneumonia / Q000000981:diagnostic imaging / Q000209:etiology / Q000401:mortality / Q000662:veterinary*; D016929:Rhodococcus equi / Q000502:physiology*; D016019:Survival Analysis; D014463:Ultrasonography
chemical_list: D006454:Hemoglobins
keywords: Age susceptibility; Experimental challenge; Foal; Pyogranulomatous pneumonia; R. equi