title: Sinus augmentation using BMP-2 in a bovine hydroxyapatite/collagen carrier in dogs.
abstract: OBJECTIVE
  The objective of this study was to determine the efficacy of bone morphogenetic protein 2 (BMP-2) in a bovine hydroxyapatite/collagen (BHC) carrier to augment bone formation in a canine nasal sinus model.
  
  
  METHODS
  Eight mongrel dogs, approximately 12 months old and 30 kg in weight were used. Following preparation of bilateral sinus access windows, BHC alone (control) or loaded with E. coli-derived BMP-2 at 0.1 mg/ml was implanted in four animals, and BHC loaded with E. coli-derived BMP-2 at 0.5 and 1.5 mg/ml was implanted in four animals. The animals were euthanized at 20 weeks when block sections were obtained for micro-computed tomography and histometric analyses.
  
  
  RESULTS
  Total augmented volumes did not differ significantly between groups. Histometric analysis showed significantly enhanced bone formation for the BMP-2 groups compared with control.
  
  
  CONCLUSIONS
  BMP-2 in a BHC carrier, even at the low 0.1-mg/ml concentration, induces osteogenic activity, enhancing local bone formation in a canine sinus model.
authors.affiliation: Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul 120-752, Republic of Korea.
authors.forename: Jae-Kook
authors.initials: JK
authors.lastname: Cha
authors.forename: Jung-Seok
authors.initials: JS
authors.lastname: Lee
authors.forename: Min-Soo
authors.initials: MS
authors.lastname: Kim
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Choi
authors.forename: Kyoo-Sung
authors.initials: KS
authors.lastname: Cho
authors.forename: Ui-Won
authors.initials: UW
authors.lastname: Jung
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage / Q000627:therapeutic use*; D002417:Cattle; D003094:Collagen / Q000737:chemistry*; D004285:Dogs; D017886:Durapatite / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods; D008443:Maxillary Sinus / Q000000981:diagnostic imaging / Q000473:pathology; D023421:Models, Animal; D009297:Nasal Mucosa / Q000000981:diagnostic imaging / Q000473:pathology; D009929:Organ Size; D010012:Osteogenesis / Q000187:drug effects; D010027:Osteotomy / Q000379:methods; D016300:Radiography, Bitewing; D059546:Sinus Floor Augmentation / Q000379:methods*; D013524:Surgical Flaps / Q000601:surgery; D054457:Tissue Scaffolds / Q000737:chemistry; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D055396:Bone Morphogenetic Protein 2; D003094:Collagen; D017886:Durapatite
keywords: bone morphogenetic protein; bone regeneration; bone tissue engineering; in vivo test; scaffold