title: A wireless and sensitive detection of octachlorostyrene using modified AuNPs as signal-amplifying tags.
abstract: A wireless, remote query octachlorostyrene (OCS) biosensor was fabricated by coating a mass-sensitive magnetoelastic ribbon with anti-OCS antibody. In response to a time-varying magnetic field, the magnetoelastic sensor mechanically vibrates at a characteristic resonance frequency which inversely depends on the sensor mass loading. As the magnetoelastic film is magnetostrictive itself, the vibrations launch magnetic flux that can be remotely detected using a pickup coil. Au nanoparticles (NPs) were used to amplify the mass loading. In a sample solution containing OCS target and OCS-modified AuNPs (OCS-AuNPs), both OCS and OCS-AuNPs react with the anti-OCS antibody immobilized on the sensor surface in a competition mode. The bound OCS-AuNPs amount is inversely proportional to the OCS target concentration. The reduction of bound OCS-AuNPs induced by free OCS results in significant change in mass loading, which amplifies the responses. The biosensor demonstrates a linear shift in resonance frequency with OCS concentration between 7.4 μM and 9 nM, with a detection limit of 2.8 nM.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, Department of Chemistry, Hunan University, Changsha 410082, People's Republic of China; Changsha Municipal Drainage Co.,Ltd., Changsha 410003, People's Republic of China.
authors.forename: Lan
authors.initials: L
authors.lastname: Chen
authors.forename: Jiezhen
authors.initials: J
authors.lastname: Li
authors.forename: T Tran
authors.initials: TT
authors.lastname: ThanhThuy
authors.forename: Liping
authors.initials: L
authors.lastname: Zhou
authors.forename: Chen'an
authors.initials: C
authors.lastname: Huang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Yuan
authors.forename: Qingyun
authors.initials: Q
authors.lastname: Cai
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D060526:Magnetic Fields; D053768:Metal Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D013343:Styrenes / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D011108:Polymers; D013343:Styrenes; C014138:octachlorostyrene; D006046:Gold
keywords: Amplify; AuNPs; Immunosensor; Octachlorostyrene; Wireless