title: Efficient enzymatic synthesis of guanosine 5'-diphosphate-sugars and derivatives.
abstract: An N-acetylhexosamine 1-kinase from Bifidobacterium infantis (NahK_15697), a guanosine 5'-diphosphate (GDP)-mannose pyrophosphorylase from Pyrococcus furiosus (PFManC), and an Escherichia coli inorganic pyrophosphatase (EcPpA) were used efficiently for a one-pot three-enzyme synthesis of GDP-mannose, GDP-glucose, their derivatives, and GDP-talose. This study represents the first facile and efficient enzymatic synthesis of GDP-sugars and derivatives starting from monosaccharides and derivatives.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University , Tianjin 300071, China, Center for Diagnostics & Therapeutics and Department of Chemistry, Georgia State University , Atlanta, Georgia 30303, United States, and Department of Chemistry, University of California , Davis, One Shields Avenue, Davis, California 95616, United States.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Liu
authors.forename: Yue
authors.initials: Y
authors.lastname: Wan
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Li
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Peng George
authors.initials: PG
authors.lastname: Wang
mesh_terms: D001644:Bifidobacterium / Q000201:enzymology*; D004926:Escherichia coli / Q000737:chemistry*; D006155:Guanosine Diphosphate Mannose / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D009005:Monosaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D010770:Phosphotransferases / Q000737:chemistry* / Q000378:metabolism; D014539:Uridine Diphosphate Sugars / Q000138:chemical synthesis* / Q000737:chemistry*
chemical_list: D009005:Monosaccharides; D014539:Uridine Diphosphate Sugars; D006155:Guanosine Diphosphate Mannose; D010770:Phosphotransferases