title: The multispecific thyroid hormone transporter OATP1C1 mediates cell-specific sulforhodamine 101-labeling of hippocampal astrocytes.
abstract: Sulforhodamine 101 (SR101) is widely used for astrocyte identification, though the labeling mechanism remains unknown and the efficacy of labeling in different brain regions is heterogeneous. By combining region-specific isolation of astrocytes followed by transcriptome analysis, two-photon excitation microscopy, and mouse genetics, we identified the thyroid hormone transporter OATP1C1 as the SR101-uptake transporter in hippocampus and cortex.
authors.affiliation: Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB), Göttingen, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Schnell
authors.forename: Ali
authors.initials: A
authors.lastname: Shahmoradi
authors.forename: Sven P
authors.initials: SP
authors.lastname: Wichert
authors.forename: Steffen
authors.initials: S
authors.lastname: Mayerl
authors.forename: Yohannes
authors.initials: Y
authors.lastname: Hagos
authors.forename: Heike
authors.initials: H
authors.lastname: Heuer
authors.forename: Moritz J
authors.initials: MJ
authors.lastname: Rossner
authors.forename: Swen
authors.initials: S
authors.lastname: Hülsmann
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000378:metabolism*; D002540:Cerebral Cortex / Q000166:cytology; D004396:Coloring Agents; D019295:Computational Biology; D004573:Electrolytes / Q000134:cerebrospinal fluid; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D062308:Optogenetics; D027701:Organic Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D012235:Rhodamines
chemical_list: D004396:Coloring Agents; D004573:Electrolytes; C464433:Oatp2 protein, mouse; D027701:Organic Cation Transport Proteins; D012235:Rhodamines; D049452:Green Fluorescent Proteins; C050829:sulforhodamine 101