title: Determination of a novel diacylglycerol acyltransferase 1 inhibitor, 2-[4-(4-{5-[2-phenyl-5-(trifluoromethyl) oxazole-4-carboxamido]-1H-benzo[d]imidazol-2-yl} phenyl) cyclohexyl] acetic acid (KR-69232) in rat plasma using liquid chromatography-tandem mass spectrometry and its application to a pharmacokinetic study.
abstract: A liquid chromatography-tandem mass spectrometry method was developed and validated for the quantification of KR-69232, a diacyltransferase 1 inhibitor, in rat plasma. KR-69232 in the concentration range of 0.004-4 µg/mL was linear. The intra-and inter-day precision and accuracy were acceptable (<20%). KR-69232 was stable under various storage and handling conditions. The method was applied successfully in a pharmacokinetic study of KR-69232 in rats.
authors.affiliation: Drug Discovery Platform Technology Group, Drug Discovery Division, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea; College of Pharmacy, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Hyewon
authors.initials: H
authors.lastname: Seo
authors.forename: Sung Heum
authors.initials: SH
authors.lastname: Choi
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Kwak
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zheng
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Kwak
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Ahn
authors.forename: Yong-Moon
authors.initials: YM
authors.lastname: Lee
authors.forename: Sung-Hoon
authors.initials: SH
authors.lastname: Ahn
authors.forename: Myung Ae
authors.initials: MA
authors.lastname: Bae
authors.forename: Jin Sook
authors.initials: JS
authors.lastname: Song
mesh_terms: D000085:Acetates / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D000818:Animals; D001562:Benzimidazoles / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D051048:Diacylglycerol O-Acyltransferase / Q000037:antagonists & inhibitors*; D004355:Drug Stability; D004791:Enzyme Inhibitors / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C000591715:2-(4-(4-(5-(2-phenyl-5-(trifluoromethyl)oxazole-4-carboxamido)-1H-benzo(d)imidazol-2-yl)phenyl)cyclohexyl)acetic acid; D000085:Acetates; D001562:Benzimidazoles; D004791:Enzyme Inhibitors; D051048:Diacylglycerol O-Acyltransferase
keywords: DGAT-1 inhibitor; LC-MS/MS; method validation; pharmacokinetics