title: Biocatalytic response of multi-layer assembled collagen/hyaluronic acid nanoengineered capsules.
abstract: Biodegradable hollow capsules filled with fluorescently labelled bovine serum albumin (BSA) as a model drug were prepared via layer-by-layer (LbL) self-assembly of type-I collagen (COL) and hyaluronic acid (HA) using calcium carbonate micro-particles and co-precipitation method. Capsules loaded with fluorescein isothiocyanate (FITC)-BSA, tetramethylrhodamin isothiocyanate (TRITC)-BSA or Alex-Fluor-488-BSA, respectively, were characterised before and after core removal using Confocal Laser Scanning Microscopy (CLSM), whilst the morphologies of individual hollow capsules were assessed using Atomic Force Microscopy (AFM). The sustained release of the encapsulated FITC-BSA protein was attained using enzymatic degradation of the capsule shells by collagenase. The released profile of the fluorescently-labelled BSA indicated that it could be successfully controlled by modulating the number of layers and/or by collagen crosslinking either before or after the capsule's assembly.
authors.affiliation: Institute for Engineering Materials and Design, University of Maribor , Maribor , Slovenia .
authors.forename: Fernanda
authors.initials: F
authors.lastname: Sousa
authors.forename: Oliver
authors.initials: O
authors.lastname: Kreft
authors.forename: Gleb B
authors.initials: GB
authors.lastname: Sukhorukov
authors.forename: Helmuth
authors.initials: H
authors.lastname: Möhwald
authors.forename: Vanja
authors.initials: V
authors.lastname: Kokol
mesh_terms: D000818:Animals; D002384:Catalysis; D002417:Cattle; D003094:Collagen / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size
chemical_list: D053769:Nanocapsules; D006820:Hyaluronic Acid; D003094:Collagen