title: Ethanolamine and choline promote expression of putative and characterized fimbriae in enterohemorrhagic Escherichia coli O157:H7.
abstract: Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is an important food-borne pathogen responsible for disease outbreaks worldwide. In order to colonize the human gastrointestinal (GI) tract and cause disease, EHEC must be able to sense the host environment and promote expression of virulence genes essential for adherence. Ethanolamine (EA) is an important metabolite for EHEC in the GI tract, and EA is also a signal that EHEC uses to activate virulence traits. Here, we report that EA influenced EHEC adherence to epithelial cells and fimbrial gene expression. Quantitative reverse transcriptase PCR indicated that EA promoted the transcription of the genes in characterized and putative fimbrial operons. Moreover, putative fimbrial structures were produced by EHEC cells grown with EA but not in medium lacking EA. Additionally, we defined two previously uncharacterized EA-regulated fimbrial operons, loc10 and loc11. We also tested whether choline or serine, both of which are also components of cell membranes, activated fimbrial gene expression. In addition to EA, choline activated fimbrial gene expression in EHEC. These findings describe for the first time the transcription of several putative fimbrial loci in EHEC. Importantly, the biologically relevant molecules EA and choline, which are abundant in the GI tract, promoted expression of these fimbriae.
authors.affiliation: Department of Microbiology, Immunology, and Cancer Biology, University of Virginia School of Medicine, Charlottesville, Virginia, USA.
authors.forename: Laura A
authors.initials: LA
authors.lastname: Gonyar
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Kendall
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects; D002794:Choline / Q000494:pharmacology*; D004847:Epithelial Cells / Q000382:microbiology; D004927:Escherichia coli Infections / Q000382:microbiology; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000235:genetics / Q000648:ultrastructure; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D019856:Ethanolamine / Q000494:pharmacology*; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism; D010861:Fimbriae, Bacterial / Q000187:drug effects* / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000187:drug effects; D046529:Microscopy, Electron, Transmission; D012694:Serine / Q000494:pharmacology
chemical_list: D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D012694:Serine; D019856:Ethanolamine; D002794:Choline