title: Ultra-high-performance liquid chromatography-tandem mass spectrometry for pharmacokinetics of bakuchiol in rats.
abstract: Psoralea Corylifolia L. is a traditional Chinese medicine with many beneficial effects in medical therapies. Bakuchiol was the main active ingredient of Psoralea Corylifolia L., used for the treatment of various diseases and also as a natural food additive. A specific and reliable ultra-high performance liquid chromatography-tandem mass spectrometry has been developed and fully validated for the quantification of bakuchiol in rat plasma. Chromatographic separation of bakuchiol and an internal standard, daidzein, was achieved on a Hypersil Gold C18 column with gradient elution that consisted of methanol and water at a flow rate of 0.2 mL/min. The compounds were detected at negative ionization mode using mass transition m/z 255.2 → 172.0 and 252.9 → 132.0 for bakuchiol and daidzein, respectively. Good linearity was obtained over the range of 2-1000 ng/mL and the lower limit of quantification was 2 ng/mL. The intra- and inter-day accuracies ranged from 91.1 to 105.7% and precisions (relative standard deviations) were within 9.3%. Bakuchiol was found to be stable under three freeze-thaw cycles, short-term temperature, post-preparative and long-term temperature conditions. The method was applied to a pharmacokinetic study of bakuchiol intravenously administered to rats at a dose of 5 mg/kg.
authors.affiliation: Department of Nutrition and Metabolism, National Institute for Nutrition and Food Safety, China Center for Disease Control and Prevention, Beijing, 100050, China.
authors.forename: Shuang-Qing
authors.initials: SQ
authors.lastname: Zhang
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Fan
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D016014:Linear Models; D008297:Male; D010636:Phenols / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010636:Phenols; C012765:bakuchiol
keywords: bakuchiol; pharmacokinetics; ultra-high performance liquid chromatography-tandem mass spectrometry