title: Oxidative stress parameters in blood, liver, and kidney of diabetic rats treated with curcumin and/or insulin.
abstract: This study evaluated the effects of curcumin and/or insulin on antioxidant enzyme activity in blood, liver, and kidney, as well as on lipid peroxidation and delta aminolevulinic dehydratase (δ-ALA-D) activity, and a histopathological analysis of streptozotocin-induced diabetic rats. The animals were divided into six groups (n = 6): control/saline (C); control/curcumin (CCur); diabetic/saline (D); diabetic/insulin (DIns); diabetic/curcumin (DCur); and diabetic/insulin/curcumin (DInsCur). After 30 days of treatment with curcumin and/or insulin, the animals were sacrificed and the liver, kidney, and serum were used for experimental determinations. Results of histopathological analysis showed that the treatment with insulin ameliorate renal and hepatic lesions from both DIns and DInsCur groups. TBARS levels were significantly increased in serum, liver, and kidney in D group and the administration of curcumin and insulin prevented this increase in DIns and DCur groups. The activities of catalase (CAT), superoxide dismutase, and δ-ALA-D presented a significant decrease in the liver and kidney D group when compared to C group (P < 0.05). The animals treated with curcumin and insulin presented an increase of CAT activity, revealing a positive interaction between both substances. The treatments with curcumin or insulin prevented oxidative stress in blood, through modulation of enzymatic antioxidant defenses. These findings contributed to the comprehension that antioxidants from medicinal plants could be used as adjuvant in the treatment of this endocrinopathy and not as single therapy.
authors.affiliation: Department of Small Animals, Hospital Veterinário, Universidade Federal de Santa Maria, Faixa de Camobi, Km 9, Avenida Roraima no 1000, Campus Universitário, Sala 103, Santa Maria, RS, 97105-900, Brazil, heinloft@hotmail.com.
authors.forename: Heloisa Einloft
authors.initials: HE
authors.lastname: Palma
authors.forename: Patrícia
authors.initials: P
authors.lastname: Wolkmer
authors.forename: Miguel
authors.initials: M
authors.lastname: Gallio
authors.forename: Marcos M B
authors.initials: MM
authors.lastname: Corrêa
authors.forename: Roberta
authors.initials: R
authors.lastname: Schmatz
authors.forename: Gustavo R
authors.initials: GR
authors.lastname: Thomé
authors.forename: Luciane B
authors.initials: LB
authors.lastname: Pereira
authors.forename: Verônica S P
authors.initials: VS
authors.lastname: Castro
authors.forename: Andréia B
authors.initials: AB
authors.lastname: Pereira
authors.forename: Andressa
authors.initials: A
authors.lastname: Bueno
authors.forename: Lizielle S
authors.initials: LS
authors.lastname: de Oliveira
authors.forename: Debora
authors.initials: D
authors.lastname: Rosolen
authors.forename: Thaís R
authors.initials: TR
authors.lastname: Mann
authors.forename: Bianca S
authors.initials: BS
authors.lastname: de Cecco
authors.forename: Dominguita L
authors.initials: DL
authors.lastname: Graça
authors.forename: Sonia T A
authors.initials: ST
authors.lastname: Lopes
authors.forename: Cinthia M A
authors.initials: CM
authors.lastname: Mazzanti
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D002374:Catalase / Q000097:blood; D003474:Curcumin / Q000008:administration & dosage*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000378:metabolism*; D007328:Insulin / Q000008:administration & dosage*; D007668:Kidney / Q000201:enzymology / Q000378:metabolism*; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress; D000623:Porphobilinogen Synthase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D007328:Insulin; D013311:Streptozocin; D002374:Catalase; D013482:Superoxide Dismutase; D000623:Porphobilinogen Synthase; D003474:Curcumin