title: Polymorphism of LRP5, but not of TNFRSF11B, is associated with a decrease in bone mineral density in postmenopausal Maya-Mestizo women.
abstract: OBJECTIVE
  Osteoporosis is a complex disease characterized principally by low bone mineral density (BMD), which is determined by an interaction of genetic, metabolic, and environmental factors. The aim of this study was to analyze the possible association among one polymorphism of LRP5 and three polymorphisms of TNFRSF11B as well as their haplotypes with BMD variations in Maya-Mestizo postmenopausal women.
  
  
  METHODS
  We studied 583 postmenopausal women of Maya-Mestizo ethnic origin. A structured questionnaire for risk factors was applied and BMD was measured in lumbar spine (LS), total hip (TH), and femoral neck (FN) by dual-energy X-ray absorptiometry. DNA was obtained from blood leukocytes. One single-nucleotide polymorphism of LRP5 (rs3736228, p.A1330V) and three of TNFRSF11B (rs4355801, rs2073618, and rs6993813) were studied using real-time PCR allelic discrimination for genotyping. Differences between the means of the BMDs according to the genotype were analyzed with covariance. Deviations from Hardy-Weinberg equilibrium were tested. Pairwise linkage disequilibrium between single nucleotide polymorphisms was calculated by direct correlation r(2), and haplotype analysis of TNFRSF11B was conducted.
  
  
  RESULTS
  The Val genotype of the rs3736228 (p.A1330V) of LRP5 was significantly associated with BMD variations at the LS, TH, and FN. None of the three polymorphisms of TNFRSF11B was associated with BMD variations.
  
  
  CONCLUSIONS
  Our results show that p.A1330V was significantly associated with BMD variations at all three skeletal sites analyzed; the Val allele and the Val/Val genotype were those most frequently found in our population.
authors.affiliation: Laboratorio de Biología de la Reproducción, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi,", Mérida Yucatán, México.
authors.forename: Thelma
authors.initials: T
authors.lastname: Canto-Cetina
authors.forename: Lucila
authors.initials: L
authors.lastname: Polanco Reyes
authors.forename: Lizbeth
authors.initials: L
authors.lastname: González Herrera
authors.forename: David
authors.initials: D
authors.lastname: Rojano-Mejía
authors.forename: Ramón Mauricio
authors.initials: RM
authors.lastname: Coral-Vázquez
authors.forename: Agustín
authors.initials: A
authors.lastname: Coronel
authors.forename: Patricia
authors.initials: P
authors.lastname: Canto
mesh_terms: D015502:Absorptiometry, Photon; D000368:Aged; D000483:Alleles; D015519:Bone Density; D001851:Bone Diseases, Metabolic / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005269:Femur / Q000502:physiology; D006239:Haplotypes; D006801:Humans; D007198:Indians, North American; D015810:Linkage Disequilibrium; D060492:Low Density Lipoprotein Receptor-Related Protein-5 / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D008159:Lumbar Vertebrae / Q000502:physiology; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D010024:Osteoporosis / Q000453:epidemiology / Q000235:genetics; D053244:Osteoprotegerin / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D017698:Postmenopause; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D011795:Surveys and Questionnaires
chemical_list: C555482:LRP5 protein, human; D060492:Low Density Lipoprotein Receptor-Related Protein-5; D053244:Osteoprotegerin; C506030:TNFRSF11B protein, human