title: New insights into mechanisms of sonothrombolysis using ultra-high-speed imaging.
abstract: Thrombotic arterial occlusion is the principal etiology for acute cardiovascular syndromes such as stroke, myocardial infarction and unstable angina. Exposing the thrombus to ultrasound and microbubbles facilitates thrombus disruption, making "sonothrombolysis" a potentially powerful therapeutic strategy for thromboembolic diseases. However, optimization of such a strategy, and hence clinical translation, is constrained by an incomplete understanding of mechanisms by which ultrasound-induced microbubble vibrations disrupt blood clots. We posit that previously reported sonothrombolytic efficacy using inertial cavitation regimes was due, at least in part, to mechanical clot disruption by oscillating microbubbles. To test this hypothesis, we optically characterized lipid microbubble interactions with thrombus in the presence of ultrasound using a recently developed ultra-high-speed microscopy imaging system to visualize microbubble acoustic behaviors at megahertz frame rates. A microscope/acoustic stage designed for the system allowed an experimentally created thrombus and microbubbles to be insonified at a co-localized acoustic and optical focus during synchronized high-speed imaging. Under inertial cavitation conditions, large-amplitude microbubble oscillations caused thrombus deformation and pitting. Acoustic radiation forces (Bjerknes forces) further augmented microbubble-thrombus interaction. These observations suggest that a direct mechanical effect of oscillating lipid microbubbles on an adjacent thrombus may play a role in mediating clot disruption in the presence of specific ultrasound conditions.
authors.affiliation: Center for Ultrasound Molecular Imaging and Therapeutics, Heart and Vascular Institute, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA.
authors.forename: Xucai
authors.initials: X
authors.lastname: Chen
authors.forename: Jonathan E
authors.initials: JE
authors.lastname: Leeman
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wang
authors.forename: John J
authors.initials: JJ
authors.lastname: Pacella
authors.forename: Flordeliza S
authors.initials: FS
authors.lastname: Villanueva
mesh_terms: D000818:Animals; D057086:High-Intensity Focused Ultrasound Ablation / Q000379:methods*; D007089:Image Enhancement / Q000379:methods*; D066298:In Vitro Techniques; D061185:Mechanical Thrombolysis / Q000379:methods*; D045423:Microbubbles / Q000627:therapeutic use*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D013927:Thrombosis / Q000000981:diagnostic imaging* / Q000628:therapy*; D016896:Treatment Outcome; D014463:Ultrasonography
keywords: High-speed imaging; Microbubble; Sonothrombolysis; Ultrasound