title: Upregulation of ATF-3 is correlated with prognosis and proliferation of laryngeal cancer by regulating Cyclin D1 expression.
abstract: OBJECTIVE
  This study aimed to investigate the expression and significance of ATF-3 in laryngeal squamous cell carcinoma (LSCC).
  
  
  METHODS
  Expression of ATF-3 was examined using immunohistochemistry methods in samples from 83 cases of LSCC carcinoma. MTT assay was used to detect proliferation of Hep-2 cells after ATF-3 knocked down by siRNA lentivirus. A mouse model was used to investigate the inhibitive role of ATF-3 siRNA in LSCC xenografts. Realtime RCR was used to detect Cyclin D1 expression after ATF-3 downregulation in Hep-2 cells.
  
  
  RESULTS
  The expression of ATF-3 was positively detected in all the 83 cases of LSCC cancer tissues while Only 4 cases of adjacent non-neoplastic tissues were detected with positive ATF-3 expression. The ATF-3 expression was statistically related with T stage, neck nodal metastasis, clinical stage and prognosis of LSCC. Both cell proliferation in vitro and tumor growth in vivo were suppressed after ATF-3 knockdown. Furthermore, the expression of Cyclin D1 was decreased after ATF-3 downregulation in Hep-2 cells.
  
  
  CONCLUSIONS
  ATF-3 is involved in the progress of LSCC, and may provide clinical information for evaluation of prognosis of LSCC. The oncologic role of ATF-3 may be correlated with Cyclin D1 regulation.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University Harbin, China.
authors.forename: Jiapeng
authors.initials: J
authors.lastname: Feng
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Sun
authors.forename: Tianyi
authors.initials: T
authors.lastname: Wu
authors.forename: Jianguang
authors.initials: J
authors.lastname: Lu
authors.forename: Lingmei
authors.initials: L
authors.lastname: Qu
authors.forename: Yanan
authors.initials: Y
authors.lastname: Sun
authors.forename: Linli
authors.initials: L
authors.lastname: Tian
authors.forename: Binghui
authors.initials: B
authors.lastname: Zhang
authors.forename: Dandan
authors.initials: D
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
mesh_terms: D051700:Activating Transcription Factor 3 / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D011379:Prognosis; D034741:RNA, Small Interfering; D015854:Up-Regulation
chemical_list: C490449:ATF3 protein, human; D051700:Activating Transcription Factor 3; D034741:RNA, Small Interfering; D019938:Cyclin D1
keywords: Cyclin D1; Laryngeal squamous cell carcinoma; activating transcription factor 3