title: Giardia lamblia transcriptome analysis using TSS-Seq and RNA-Seq.
abstract: Giardia lamblia is a protozoan parasite that is found worldwide and has both medical and veterinary importance. We applied the transcription start sequence (TSS-seq) and RNA sequence (RNA-seq) techniques to study the transcriptome of the assemblage A WB strain trophozoite. We identified 8000 transcription regions (TR) with significant transcription. Of these regions, 1881 TRs were more than 500 nucleotides upstream of an annotated ORF. Combining both techniques helped us to identify 24 ORFs that should be re-annotated and 60 new ORFs. From the 8000 TRs, we were able to identify an AT-rich consensus that includes the transcription initiation site. It is possible that transcription that was previously thought to be bidirectional is actually unidirectional.
authors.affiliation: Department of Medical Genomics, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo, Japan ; Department of Parasitology, Faculty of Medicine, Assiut University, Assiut, Egypt.
authors.forename: Mohammed E M
authors.initials: ME
authors.lastname: Tolba
authors.forename: Seiki
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Mihoko
authors.initials: M
authors.lastname: Imada
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Sumio
authors.initials: S
authors.lastname: Sugano
mesh_terms: D001483:Base Sequence; D020869:Gene Expression Profiling / Q000379:methods*; D017125:Genes, Protozoan / Q000235:genetics; D016829:Giardia lamblia / Q000235:genetics*; D008957:Models, Genetic; D016366:Open Reading Frames / Q000235:genetics; D016053:RNA, Protozoan / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA / Q000379:methods*; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
chemical_list: D016053:RNA, Protozoan