title: Phosphorylation of nucleoside-metallacarborane and carborane conjugates by nucleoside kinases.
abstract: A library of purine and pyrimidine nucleosides modified with carborane or metallacarborane boron clusters at different locations, consisting of new molecules as well as already described compounds, was prepared. The compounds were tested as substrates for human deoxynucleoside kinases. Some conjugates, with modification attached to N3 of thymidine via a linker containing the triazole moiety, were efficiently phosphorylated by cytosolic thymidine kinase 1 and mitochondrial thymidine kinase 2. Higher phosphorylation levels were observed with thymidine kinase 1, the phosphorylation of nucleosides modified with metallacarboranes was observed for the first time.
authors.affiliation: a Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences , Lodz , Poland.
authors.forename: Blazej A
authors.initials: BA
authors.lastname: Wojtczak
authors.forename: Agnieszka B
authors.initials: AB
authors.lastname: Olejniczak
authors.forename: Liya
authors.initials: L
authors.lastname: Wang
authors.forename: Staffan
authors.initials: S
authors.lastname: Eriksson
authors.forename: Zbigniew J
authors.initials: ZJ
authors.lastname: Lesnikowski
mesh_terms: D001895:Boron / Q000737:chemistry*; D006801:Humans; D008958:Models, Molecular; D008968:Molecular Conformation; D009705:Nucleosides / Q000737:chemistry* / Q000378:metabolism*; D009942:Organometallic Compounds / Q000737:chemistry*; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism*
chemical_list: D009705:Nucleosides; D009942:Organometallic Compounds; D010770:Phosphotransferases; C021892:nucleoside phosphotransferase; D001895:Boron