title: Role of alternative polyadenylation during adipogenic differentiation: an in silico approach.
abstract: Post-transcriptional regulation of stem cell differentiation is far from being completely understood. Changes in protein levels are not fully correlated with corresponding changes in mRNAs; the observed differences might be partially explained by post-transcriptional regulation mechanisms, such as alternative polyadenylation. This would involve changes in protein binding, transcript usage, miRNAs and other non-coding RNAs. In the present work we analyzed the distribution of alternative transcripts during adipogenic differentiation and the potential role of miRNAs in post-transcriptional regulation. Our in silico analysis suggests a modest, consistent, bias in 3'UTR lengths during differentiation enabling a fine-tuned transcript regulation via small non-coding RNAs. Including these effects in the analyses partially accounts for the observed discrepancies in relative abundance of protein and mRNA.
authors.affiliation: Bioinformatics Unit, Institut Pasteur Montevideo, Montevideo, Uruguay.
authors.forename: Lucía
authors.initials: L
authors.lastname: Spangenberg
authors.forename: Alejandro
authors.initials: A
authors.lastname: Correa
authors.forename: Bruno
authors.initials: B
authors.lastname: Dallagiovanna
authors.forename: Hugo
authors.initials: H
authors.lastname: Naya
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D050156:Adipogenesis / Q000235:genetics*; D019295:Computational Biology; D005786:Gene Expression Regulation; D016014:Linear Models; D035683:MicroRNAs / Q000235:genetics; D026723:Polyadenylation / Q000235:genetics; D011506:Proteins / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D011506:Proteins