title: Ascorbic acid glucoside reduces neurotoxicity and glutathione depletion in mouse brain induced by nitrotriazole radiosensitazer.
abstract: OBJECTIVE
  To investigate the potential of the anti-oxidant ascorbic acid glucoside (AA-2G) to modulate neurotoxicity induced by high doses of nitrotriazole radiosensitizer.
  
  
  METHODS
  Male and female C56Bl/6xCBA hybrid mice aged 8-14 weeks (weight 18-24 g) were used. Nitrotriazole drug radiosensitizer sanazole at a high dose of 2, 1 g/kg was per os administered to induce neurotoxicity at mice. Ascorbic acid glucoside was given 30 min before the sanazole administration. Serum ascorbic acid, brain glutathione level, as well as behavioral performance using open field apparatus were measured.
  
  
  RESULTS
  Administration of high (non-therapeutic) doses of the nitrotriazole drug sanazole results in neurotoxicity in mice as evidenced from behavioral performance, emotional activity and depletion of the cellular antioxidant, glutathione, in the brain. The serum levels of ascorbic acid was also found reduced in high dose sanazole treated animals. Per os administration of ascorbic acid glucoside significantly reduced the neurotoxicity. This effect was associated with the prevention of glutathione depletion in mouse brain and restoring the ascorbic acid level in serum.
  
  
  CONCLUSIONS
  Administration of ascorbic acid glucoside, but not ascorbic acid, before sanazole administration protected from sanazole-induced neurotoxicity by preventing the decrease in the brain reduced glutathione level and providing high level of ascorbic acid in plasma.
authors.affiliation: Cancer Research Institute of Siberian Branch of the Russian Acad. Med. Sci, Tomsk, Russia.
authors.forename: Nadezda V
authors.initials: NV
authors.lastname: Cherdyntseva
authors.forename: Anna A
authors.initials: AA
authors.lastname: Ivanova
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Ivanov
authors.forename: Evgeny
authors.initials: E
authors.lastname: Cherdyntsev
authors.forename: Cherupally Krishnan Krishnan
authors.initials: CK
authors.lastname: Nair
authors.forename: Tsutomu V
authors.initials: TV
authors.lastname: Kagiya
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D005260:Female; D005960:Glucosides / Q000008:administration & dosage / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism*; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D011838:Radiation-Sensitizing Agents / Q000009:adverse effects* / Q000633:toxicity; D014230:Triazoles / Q000009:adverse effects* / Q000633:toxicity
chemical_list: D005960:Glucosides; D011838:Radiation-Sensitizing Agents; D014230:Triazoles; C072690:AK 2123; D005978:Glutathione; D001205:Ascorbic Acid