title: Microbial recycling of glycerol to biodiesel.
abstract: The sustainable supply of lipids is the bottleneck for current biodiesel production. Here microbial recycling of glycerol, byproduct of biodiesel production to biodiesel in engineered Escherichia coli strains was reported. The KC3 strain with capability of producing fatty acid ethyl esters (FAEEs) from glucose was used as a starting strain to optimize fermentation conditions when using glycerol as sole carbon source. The YL15 strain overexpressing double copies of atfA gene displayed 1.7-fold increase of FAEE productivity compared to the KC3 strain. The titer of FAEE in YL15 strain reached to 813 mg L(-1) in minimum medium using glycerol as sole carbon source under optimized fermentation conditions. The titer of glycerol-based FAEE production can be significantly increased by both genetic modifications and fermentation optimization. Microbial recycling of glycerol to biodiesel expands carbon sources for biodiesel production.
authors.affiliation: Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao 266101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: yangliu@qibebt.ac.cn.
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhu
authors.forename: Weihua
authors.initials: W
authors.lastname: Wang
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Lu
mesh_terms: D061249:Batch Cell Culture Techniques; D056804:Biofuels / Q000382:microbiology* / Q000600:supply & distribution; D053898:Biosynthetic Pathways / Q000187:drug effects / Q000235:genetics; D001711:Biotransformation; D003470:Culture Media / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D004951:Esterification / Q000187:drug effects; D004952:Esters / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D005990:Glycerol / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D059027:Recycling
chemical_list: D056804:Biofuels; D003470:Culture Media; D004952:Esters; D005227:Fatty Acids; D000431:Ethanol; D005990:Glycerol
keywords: Biodiesel; Escherichia coli; Fermentation; Genetic engineering; Glycerol