title: Degree of discrepancy between HbA1c and glycemia in variant hemoglobin is smaller when HbA1c is measured by new-type Arkray HPLC compared with old-type HPLC.
abstract: OBJECTIVE
  Although variant hemoglobin mainly demonstrates inappropriate HbA1c values measured by high-performance liquid chromatography (HPLC), these values differ depending on the HPLC model. In the 1990s, old-type HPLC models were replaced with new-type HPLC models which could separate stable HbA1c from labile HbA1c and modified hemoglobin. This study compared HbA1c values in subjects with variant hemoglobin measured using old-type Arkray HPLC (HA-8150) and new-type Arkray HPLC (HA-8160 or HA-8180).
  
  
  METHODS
  This study included non-diabetic subjects with apparently low HbA1c values who had variant hemoglobins due to a β-chain heterozygous mutation. HbA1c was measured by old-type HPLC in 28 subjects with 12 variant hemoglobins (group 1) and new-type HPLC in six subjects with four variant hemoglobins (group 2). When HbA1c was measured by HPLC (HPLC-HbA1c), HbA1c measured by immunoassay (IA-HbA1c) and glycated albumin (GA) were also measured.
  
  
  RESULTS
  IA-HbA1c and GA did not significantly differ between both groups. However, HPLC-HbA1c in group 2 was significantly higher than that in group 1 (group 1: 2.9 ± 0.7% vs. group 2: 3.7 ± 0.2%, P = 0.006).
  
  
  CONCLUSIONS
  When HbA1c in subjects with variant hemoglobin is measured by new-type Arkray HPLC, the degree of discrepancy between HbA1c and glycemia is smaller compared with that measured by old-type HPLC.
authors.affiliation: Department of Internal Medicine, Kawanishi City Hospital, Hyogo, Japan. Electronic address: m-koga@kawanishi-city-hospital.com.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Koga
authors.affiliation: Department of Internal Medicine, Nishinomiya Municipal Hospital, Hyogo, Japan.
authors.forename: Shogo
authors.initials: S
authors.lastname: Kurebayashi
authors.affiliation: Department of Internal Medicine, Kawasaki Hospital, Osaka, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Murai
authors.affiliation: Department of Internal Medicine, Kinki Central Hospital, Hyogo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Saito
authors.affiliation: Department of Central Laboratory, Osaka Medical College, Osaka, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Miyazaki
mesh_terms: D001786:Blood Glucose / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006442:Glycated Hemoglobin / Q000378:metabolism*; D006455:Hemoglobins, Abnormal / Q000378:metabolism*; D006801:Humans
chemical_list: D001786:Blood Glucose; D006442:Glycated Hemoglobin; D006455:Hemoglobins, Abnormal; C517652:hemoglobin A1c protein, human
keywords: Glycated albumin; HbA1c; High-performance liquid chromatography; Variant hemoglobin