title: Rate control of cell sheet recovery by incorporating hydrophilic pattern in thermoresponsive cell culture dish.
abstract: Thready stripe-polyacrylamide (PAAm) pattern was fabricated on a thermoresponsive poly(N-isopropylacrylamide) (PIPAAm) surface, and their surface properties were characterized. A PIPAAm surface spin-coated with positive photoresist was irradiated through a 5 µm/5 µm or a 10 µm/10-µm black and white striped photomask, resulting in the radical polymerization of AAm on the photoirradiated area. After staining with Alexa488 bovine serum albumin, the stripe-patterned surface was clearly observed and the patterned surface was also observed by a phase contrast image of an atomic force microscope. NIH-3T3 (3T3) single cells were able to be cultured at 37°C on the patterned surfaces as well as on a PIPAAm surface without pattern, and the detachment of adhered cells was more rapidly from the patterned surface after reducing temperature. Furthermore, the rate of detachment of 3T3 confluent cell sheet on the patterned surface was accelerated, compared with on a conventional PIPAAm surface under the static condition. The rate control of cell sheet recovery should contribute the preservations of cell phenotype and biological functions of cell sheet for applying to clinical trials.
authors.affiliation: Institute of Advanced Biomedical Engineering and Science (TWIns), Tokyo Women's Medical University, 8-1 Kawadacho, Shinjuku-ku, Tokyo, 162-8666, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kumashiro
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Matsunaga
authors.forename: Megumi
authors.initials: M
authors.lastname: Muraoka
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Tanaka
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Itoga
authors.forename: Jun
authors.initials: J
authors.lastname: Kobayashi
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Tomiyama
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Kuroda
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Shimizu
authors.forename: Iwao
authors.initials: I
authors.lastname: Hashimoto
authors.forename: Kazuo
authors.initials: K
authors.lastname: Umemura
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D000180:Acrylic Resins / Q000494:pharmacology; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D041681:NIH 3T3 Cells; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D000099166:Polyacrylamides; C052970:poly-N-isopropylacrylamide
keywords: cell adhesion; cell detachment; cell sheet; micropatterning; thermoresponsive surface