title: DNA detection and cell adhesion on plasma-polymerized pyrrole.
abstract: This study investigates the application of Plasma-polymerized pyrrole (ppPY) as bioactive platform for DNA immobilization and cell adhesion based on the fundamental properties of ppPY, such as chemical structure, electrochemical property, and protein adsorption. Variations in electrochemical properties of the ppPY film deposited under different plasma conditions before and after DNA immobilization were measured using electrochemical impedance spectroscopy (EIS). The equilibrium concentration of the probe DNA immobilized on the ppPY surface was deduced by detecting the variations in the surface charge transfer resistance (Rct ) of the ppPY films after DNA immobilization with different concentrations. In addition, the detection limit of the target DNA hybridization with probe DNA, the association constant, Ka , and the dissociation constant were deduced from Langmuir isotherm equations simulated using the experimental data collected by EIS. Moreover, inverted microscope was used to observe the cell adhesions onto the surface of the ppPY films prepared under different plasma conditions. Different adhesive behaviors of cells were observed, demonstrating that ppPY films could be an alternative biomaterial used as the sensitive layer for DNA sensor or cell adhesion.
authors.affiliation: Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 5 Dong Feng Rd., Zhengzhou, 450002, People's Republic of China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Shunli
authors.initials: S
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Shi
authors.forename: Jun
authors.initials: J
authors.lastname: Dou
authors.forename: Shaoming
authors.initials: S
authors.lastname: Fang
mesh_terms: D002138:Calibration; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000378:metabolism; D058266:Dielectric Spectroscopy; D004566:Electrodes; D006801:Humans; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D058626:Plasma Gases / Q000737:chemistry*; D058105:Polymerization; D011758:Pyrroles / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D058626:Plasma Gases; D011758:Pyrroles; D004247:DNA
keywords: DNA immobilization/hybridization; cell adhesion; electrochemical impedance spectroscopy; plasma polymerization