title: Complete mitochondrial genome of the desert toad-headed agama, Phrynocephalus przewalskii (Reptilia, Squamata, Agamidae), a novel gene organization in vertebrate mtDNA.
abstract: The mitogenome of the desert toad-headed agama, Phrynocephalus przewalskii, was amplified using polymerase chain reaction (PCR), long-and-accurate PCR and directly sequenced by primer walking. The complete mitogenome was 16,892 bp in size and contained 13 protein-coding, 23 tRNA, and 2 rRNA genes, and 1 control region. The mitogenome of the P. przewalskii was similar to those of other Phrynocephalus sand lizards in gene arrangement and composition, except that tRNA-Phe and tRNA-Pro were exchanged and tRNA-Phe had two copies. The control region comprised three parts, one between tRNA-Thr and tRNA-Phe, a second between the two tRNA-Phe copies, and a third between tRNA-Pro and 12S RNA. The overall nucleotide composition of the H-strand was 36.3% A, 26.7% T, 12.5% G, 24.6% C. The complete mitogenome of P. przewalskii will contribute to understanding the evolution of the genus Phrynocephalus and the family Agamidae.
authors.affiliation: a School of Life Sciences, Lanzhou University , Lanzhou , China.
authors.forename: Donghai
authors.initials: D
authors.lastname: Li
authors.affiliation: a School of Life Sciences, Lanzhou University , Lanzhou , China.
authors.forename: Sen
authors.initials: S
authors.lastname: Song
authors.affiliation: a School of Life Sciences, Lanzhou University , Lanzhou , China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: a School of Life Sciences, Lanzhou University , Lanzhou , China.
authors.forename: Caihong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: a School of Life Sciences, Lanzhou University , Lanzhou , China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Chang
mesh_terms: D000818:Animals; D001482:Base Composition; D004272:DNA, Mitochondrial / Q000032:analysis; D023061:Gene Order; D059646:Genome Size; D054629:Genome, Mitochondrial; D008928:Mitochondria / Q000235:genetics*; D010802:Phylogeny; D012104:Reptiles / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial
keywords: Genome organization; Phrynocephalus przewalskii; mitogenome