title: Gastric autoantigenic proteins in Helicobacter pylori infection.
abstract: OBJECTIVE
  This study tried to identify novel gastric autoimmune antigens that might be involved in aggravating the atrophic gastritis among patients with Helicobacter pylori infection using two-dimensional immunoblotting analysis.
  
  
  METHODS
  Proteins from gastric mucosal antrectomy specimens and AGS cells (gastric adenocarcinoma cell lines derived from a Caucasian patient who had received no prior therapy) were 2-dimensionally immunoblotted separately with a pool of 300 sera from H. pylroi-infected patients at Gyeongsang National University Hospital.
  
  
  RESULTS
  Thirty-eight autoantigenic proteins including alcohol dehydrogenase [NADP+], alpha enolase, gastrokine-1, gastric triacylglycerol lipase, heat shock 70 kDa protein 1, and peroxiredoxin-2 were identified in the gastric mucosal tissue. Fourteen autoantigenic proteins including programmed cell death 6-interacting protein, serum albumin and T-complex protein 1 subunit gamma were identified in the AGS cells. Albumin, alpha-enolase, annexin A3, cytoplasmic actin 1, heat shock cognate 71 kDa protein and leukocyte elastase inhibitor were commonly observed autoantigenic proteins in both gastric mucosal tissue and AGS cells. Alpha-enolase, glutathione S-transferase P, heat shock cognate 71 kDa protein, heat shock 70 kDa protein 1, human mitochondrial adenosine triphosphate synthase (ATP) subunit beta, mitochondrial 60 kDa heat shock protein, peroxiredoxin-2, 78 kDa glucose-regulated protein precursor, tyrosine-protein phosphatase non-receptor type 11 and Tryptophan-Aspartic acid (WD) repeat-containing protein 1 showed 60% or higher amino acid positivity.
  
  
  CONCLUSIONS
  These newly identified gastric autoimmune antigens might be useful in the control and prevention of gastroduodenal disorders, and might be valuable in breaking the vicious circle that exists in gastroduodenal disorders if their pathophysiological roles could be understood in the progress of chronic atrophic gastritis, gastroduodenal ulcers, intestinal metaplasia, and gastric carcinogenesis.
authors.affiliation: Department of Pediatrics, Gyeongsang National University School of Medicine, 79 Gangnam-ro, Jinju 660-702, Korea. hsyoun@gnu.ac.kr.
authors.forename: Ji Sook
authors.initials: JS
authors.lastname: Park
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Tae Hyo
authors.initials: TH
authors.lastname: Kim
authors.forename: Jeongsuk
authors.initials: J
authors.lastname: Yeom
authors.forename: Eun-Sil
authors.initials: ES
authors.lastname: Park
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Seo
authors.forename: Jin-Su
authors.initials: JS
authors.lastname: Jun
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Lim
authors.forename: Chan-Hoo
authors.initials: CH
authors.lastname: Park
authors.forename: Hyang-Ok
authors.initials: HO
authors.lastname: Woo
authors.forename: Hee-Shang
authors.initials: HS
authors.lastname: Youn
authors.forename: Gyung-Hyuck
authors.initials: GH
authors.lastname: Ko
authors.forename: Hyung-Lyun
authors.initials: HL
authors.lastname: Kang
authors.forename: Seung-Chul
authors.initials: SC
authors.lastname: Baik
authors.forename: Woo-Kon
authors.initials: WK
authors.lastname: Lee
authors.forename: Myung-Je
authors.initials: MJ
authors.lastname: Cho
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Rhee
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism; D001324:Autoantigens / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005753:Gastric Mucosa / Q000378:metabolism / Q000382:microbiology; D016481:Helicobacter Infections / Q000378:metabolism*; D006801:Humans; D036361:Peptide Hormones / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000378:metabolism
chemical_list: D001324:Autoantigens; C477000:GKN1 protein, human; D036361:Peptide Hormones; D000426:Alcohol Dehydrogenase; D010751:Phosphopyruvate Hydratase
keywords: 2D immunoblotting; Helicobacter pylori; autoantigen; gastric atrophy