title: Association between clinical pathology and multiple genes mRNA expression in Chinese patients with NSCLC.
abstract: OBJECTIVE
  The aim of this study was to evaluate whether there was an association between pathology type and ERCC1, BRCA1, RRM1, TUBB3, STMN1, TOP2A and epidermal growth factor receptor (EGFR) messenger ribonucleic acid (mRNA) expression level in Chinese patients with non-small cell lung carcinoma (NSCLC).
  
  
  METHODS
  mRNA expression level of these genes was analyzed in 181 cancer tissues by using xTAG-step liquid-chip array. The mRNA expression level of the seven genes was evaluated in association with the clinical pathology type.
  
  
  RESULTS
  The average mRNA expression level of the seven genes were ERCC1 (1.02 ± 0.03), BRCA1 (0.15 ± 0.04), RRM1 (0.19 ± 0.05), TUBB3 (0.31 ± 0.06), STMN1 (2.78 ± 0.42), TOP2A (3.04 ± 0.42) and EGFR (0.58 ± 0.09), respectively in Chinese patients with NSCLC. The mRNA expression level of ERCC1, STMN1 and TOP2A genes were statistical different with different pathology type (p(a) < 0.05); STMN1 and TOP2A genes mRNA expression were much higher in squamous cell lung carcinoma than that in non-squamous cell lung carcinoma (p(a) < 0.05). And ERCC1 gene expression was much lower in squamous cell carcinoma than that in non-squamous cell carcinoma (p(a) < 0.05).
  
  
  CONCLUSIONS
  mRNA expression level of STMN1, TOP2A and ERCC1 were correlated with the clinical pathology type.
authors.affiliation: Department of Lung Cancer Surgery, Tianjin Medical University General Hospital, Tianjin, 300052, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: G U
authors.initials: GU
authors.lastname: Jundong
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Zuoqing
authors.initials: Z
authors.lastname: Song
mesh_terms: D000328:Adult; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism*; D002681:China; D004250:DNA Topoisomerases, Type II / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D000075223:Poly-ADP-Ribose Binding Proteins; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D050777:Stathmin / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; D000075223:Poly-ADP-Ribose Binding Proteins; D012333:RNA, Messenger; C493995:STMN1 protein, human; D050777:Stathmin; C071447:ERCC1 protein, human; D004720:Endonucleases; D004250:DNA Topoisomerases, Type II; C000618811:TOP2A protein, human