title: Epicardial delivery of collagen patches with adipose-derived stem cells in rat and minipig models of chronic myocardial infarction.
abstract: Although transplantation of adipose-derived stem cells (ADSC) in chronic myocardial infarction (MI) models is associated with functional improvement, its therapeutic value is limited due to poor long-term cell engraftment and survival. Thus, the objective of this study was to examine whether transplantation of collagen patches seeded with ADSC could enhance cell engraftment and improve cardiac function in models of chronic MI. With that purpose, chronically infarcted Sprague-Dawley rats (n = 58) were divided into four groups and transplanted with media, collagen scaffold (CS), rat ADSC, or CS seeded with rat ADSC (CS-rADSC). Cell engraftment, histological changes, and cardiac function were assessed 4 months after transplantation. In addition, Göttingen minipigs (n = 18) were subjected to MI and then transplanted 2 months later with CS or CS seeded with autologous minipig ADSC (CS-pADSC). Functional and histological assessments were performed 3 months post-transplantation. Transplantation of CS-rADSC was associated with increased cell engraftment, significant improvement in cardiac function, myocardial remodeling, and revascularization. Moreover, transplantation of CS-pADSC in the pre-clinical swine model improved cardiac function and was associated with decreased fibrosis and increased vasculogenesis. In summary, transplantation of CS-ADSC resulted in enhanced cell engraftment and was associated with a significant improvement in cardiac function and myocardial remodeling.
authors.affiliation: Laboratory of Cell Therapy, Division of Oncology, Foundation for Applied Medical Research, University of Navarra, Spain.
authors.forename: Miriam
authors.initials: M
authors.lastname: Araña
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Gavira
authors.forename: Estefanía
authors.initials: E
authors.lastname: Peña
authors.forename: Arantxa
authors.initials: A
authors.lastname: González
authors.forename: Gloria
authors.initials: G
authors.lastname: Abizanda
authors.forename: Myriam
authors.initials: M
authors.lastname: Cilla
authors.forename: Marta M
authors.initials: MM
authors.lastname: Pérez
authors.forename: Edurne
authors.initials: E
authors.lastname: Albiasu
authors.forename: Natalia
authors.initials: N
authors.lastname: Aguado
authors.forename: Mayte
authors.initials: M
authors.lastname: Casado
authors.forename: Begoña
authors.initials: B
authors.lastname: López
authors.forename: Susana
authors.initials: S
authors.lastname: González
authors.forename: Mario
authors.initials: M
authors.lastname: Soriano
authors.forename: Cristina
authors.initials: C
authors.lastname: Moreno
authors.forename: Juana
authors.initials: J
authors.lastname: Merino
authors.forename: José M
authors.initials: JM
authors.lastname: García-Verdugo
authors.forename: Javier
authors.initials: J
authors.lastname: Díez
authors.forename: Manuel
authors.initials: M
authors.lastname: Doblaré
authors.forename: Beatriz
authors.initials: B
authors.lastname: Pelacho
authors.forename: Felipe
authors.initials: F
authors.lastname: Prosper
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002908:Chronic Disease; D003094:Collagen / Q000008:administration & dosage*; D004195:Disease Models, Animal; D006321:Heart / Q000503:physiopathology; D009203:Myocardial Infarction / Q000503:physiopathology / Q000601:surgery*; D010496:Pericardium; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation; D013552:Swine; D013556:Swine, Miniature; D054457:Tissue Scaffolds
chemical_list: D003094:Collagen
keywords: Adipose-derived stem cells (ADSC); Biocompatibility; Collagen carriers; Myocardial infarction; Preclinical model