title: B7-H1 expression is associated with poor prognosis in colorectal carcinoma and regulates the proliferation and invasion of HCT116 colorectal cancer cells.
abstract: OBJECTIVE
  The investigation concerning the B7-H1 expression in colorectal cancer cells is at an early stage. It is unclear whether B7-H1 expression may have diagnostic or prognostic value in colorectal carcinoma. Additionally, how B7-H1 is associated with the clinical features of colorectal carcinoma is not known. In order to investigate the relationship between B7-H1 and colorectal cancer, we analyzed B7-H1 expression and its effect in clinical specimens and HCT116 cells.
  
  
  METHODS
  Paraffin-embedded specimens from 143 eligible patients were used to investigate the expression of CD274 by immunohistochemistry. We also examined whether B7-H1 itself may be related to cell proliferation, apoptosis, migration and invasion in colon cancer HCT116 cells.
  
  
  RESULTS
  Our results show that B7-H1 was highly expressed in colorectal carcinoma and was significantly associated with cell differentiation status and TNM (Tumor Node Metastasis) stage. Patients with positive B7-H1 expression showed a trend of shorter survival time. Using multivariate analysis, we demonstrate that positive B7-H1 expression is an independent predictor of colorectal carcinoma prognosis. Our results indicate that B7-H1 silencing with siRNA inhibits cell proliferation, migration and invasion. Furthermore, cell apoptosis was also increased by B7-H1 inhibition.
  
  
  CONCLUSIONS
  Positive B7-H1 expression is an independent predictor for colorectal carcinoma prognosis. Moreover, knockdown of B7-H1 can inhibit cell proliferation, migration and invasion.
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Immunology, Fourth Military Medical University, Xi'an, China.
authors.forename: Sheng-Jia
authors.initials: SJ
authors.lastname: Shi
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Wang
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Wang
authors.forename: Zhang-Yan
authors.initials: ZY
authors.lastname: Guo
authors.forename: Ming
authors.initials: M
authors.lastname: Wei
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Meng
authors.forename: An-Gang
authors.initials: AG
authors.lastname: Yang
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Wen
mesh_terms: D017209:Apoptosis / Q000502:physiology; D060890:B7-H1 Antigen / Q000378:metabolism; D015153:Blotting, Western; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D045325:HCT116 Cells; D006801:Humans; D007150:Immunohistochemistry
chemical_list: D060890:B7-H1 Antigen