title: Continuous co-fermentation of cellobiose and xylose by engineered Saccharomyces cerevisiae.
abstract: Simultaneous fermentation of cellobiose and xylose by an engineered Saccharomyces cerevisiae has been demonstrated in batch fermentation, suggesting the feasibility of continuous co-fermentation of cellulosic sugars. As industrial S. cerevisiae strains have known to possess higher ethanol productivity and robustness compared to laboratory S. cerevisiae strains, xylose and cellobiose metabolic pathways were introduced into a haploid strain derived from an industrial S. cerevisiae. The resulting strain (JX123-BTT) was able to ferment a mixture of cellobiose and xylose simultaneously in batch fermentation with a high ethanol yield (0.38 g/g) and productivity (2.00 g/L · h). Additionally, the JX123-BTT strain co-consumed glucose, cellobiose, and xylose under continuous culture conditions at a dilution rate of 0.05 h(-1) and produced ethanol resulting in 0.38 g/g of ethanol yield and 0.96 g/L · h of productivity. This is the first demonstration of co-fermentation of cellobiose and xylose by an engineered S. cerevisiae under continuous culture conditions.
authors.affiliation: Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Republic of Korea.
authors.forename: Suk-Jin
authors.initials: SJ
authors.lastname: Ha
authors.forename: Soo Rin
authors.initials: SR
authors.lastname: Kim
authors.forename: Heejin
authors.initials: H
authors.lastname: Kim
authors.forename: Jing
authors.initials: J
authors.lastname: Du
authors.forename: Jamie H D
authors.initials: JH
authors.lastname: Cate
authors.forename: Yong-Su
authors.initials: YS
authors.lastname: Jin
mesh_terms: D002475:Cellobiose / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D007218:Industrial Microbiology; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
chemical_list: D002475:Cellobiose; D014994:Xylose
keywords: Cellobiose; Cellodextrin transporter; Continuous co-fermentation; Industrial Saccharomyces cerevisiae; Xylose