title: Data-independent acquisition (MSE) with ion mobility provides a systematic method for analysis of a bacteriophage structural proteome.
abstract: In this work, a method was developed to study the structural proteome of mycobacteriophage Marvin, a recent isolate from soil with 107 predicted coding sequences. This prototype method was applied for semi-quantitative analysis of the composition of this mycobacteriophage virion using ion mobility spectrometry and data-independent acquisition (MS(E)-IMS). MS(E)-IMS was compared to a more conventional proteomics technique employing mass spectrometry with a data-dependent acquisition strategy. MS(E)-IMS provided broad coverage of the virion proteome and high sequence coverage for individual proteins. This shotgun method does not depend on the limited sensitivity of visualization of protein bands by staining reagents inherent in gel-based methods. The method is comprehensive, provides high sequence coverage and is proposed as a particularly efficient method for the study of bacteriophage proteomes.
authors.affiliation: Cabrini College, Department of Science, 610 King of Prussia Road, Radnor, PA 19087, United States.
authors.forename: Deborah
authors.initials: D
authors.lastname: Moran
authors.forename: Trevor
authors.initials: T
authors.lastname: Cross
authors.forename: Lewis M
authors.initials: LM
authors.lastname: Brown
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Colligan
authors.forename: David
authors.initials: D
authors.lastname: Dunbar
mesh_terms: D013058:Mass Spectrometry / Q000379:methods*; D009160:Mycobacteriophages / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D015678:Viral Structural Proteins / Q000032:analysis*
chemical_list: D020543:Proteome; D015678:Viral Structural Proteins
keywords: Bacteriophage; HDMS; Ion mobility; MS(E); Mass spectrometry; Shotgun proteomics; Structural proteome; Traveling wave ion mobility spectrometry (TWIMS); Virion proteomics