title: Novel cancer-targeting SPECT/NIRF dual-modality imaging probe (99m)Tc-PC-1007: synthesis and biological evaluation.
abstract: Synthesis, characterization, in vitro and in vivo biological evaluation of a heptamethine cyanine based dual-mode single-photon emission computed tomography (SPECT)/near infrared fluorescence (NIRF) imaging probe (99m)Tc-PC-1007 is described. (99m)Tc-PC-1007 exhibited preferential accumulation in human breast cancer MCF-7 cells. Cancer-specific SPECT/CT and NIRF imaging of (99m)Tc-PC-1007 was performed in a breast cancer xenograft model. The probe uptake ratio of tumor to control (spinal cord) was calculated to be 4.02±0.56 at 6 h post injection (pi) and 8.50±1.41 at 20 h pi (P<0.0001). Pharmacokinetic parameters such as blood clearance and organ distribution were assessed.
authors.affiliation: Department of Radiology and Medical Imaging, University of Virginia, Rm 263, 480 Ray C. Hunt Drive, Snyder Building, Charlottesville, VA 22908, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.forename: Kosta
authors.initials: K
authors.lastname: Popovic
authors.forename: Xiuzhen
authors.initials: X
authors.lastname: Xie
authors.forename: Mahendra D
authors.initials: MD
authors.lastname: Chordia
authors.forename: Leland W K
authors.initials: LW
authors.lastname: Chung
authors.forename: Mark B
authors.initials: MB
authors.lastname: Williams
authors.forename: Wei
authors.initials: W
authors.lastname: Yue
authors.forename: Dongfeng
authors.initials: D
authors.lastname: Pan
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000378:metabolism; D005260:Female; D064593:Heterografts; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D064847:Multimodal Imaging / Q000379:methods; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000493:pharmacokinetics; D019265:Spectroscopy, Near-Infrared; D017556:Technetium Compounds / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods; D023041:Xenograft Model Antitumor Assays
chemical_list: D019275:Radiopharmaceuticals; D017556:Technetium Compounds
keywords: 99m-Technetium; Breast cancer; Dual-modality; Heptamethine cyanine dye; Near infrared fluorescence; SPECT