title: Using stable isotope probing to obtain a targeted metatranscriptome of aerobic methanotrophs in lake sediment.
abstract: In this study, we demonstrate the possibility of obtaining a targeted metatranscriptome from a functional group of microorganisms using a stable isotope probing (SIP) approach. Methanotrophs in lake sediment were labelled using (13)CH4, and both labelled and unlabelled-RNA were isolated and sequenced by 454 pyrosequencing. The unlabelled metatranscriptome had a large diversity of bacterial, archaeal, eukaryotic and viral sequences as expected from a diverse sediment community. In contrast, the labelled-RNA metatranscriptome was dominated by methanotroph sequences, particularly from Methylococcaceae. Transcripts of the methane monooxygenase genes pmoCAB were the most abundant in this metatranscriptome, and the pathway of methane oxidation to CO2 could be traced, as well as many steps in the ribulose monophosphate pathway for carbon assimilation. A high abundance of mRNA transcripts for proteins related to motility was detected, suggesting an importance for methanotrophs in lake sediments. This combination of SIP and metatranscriptomics should be broadly applicable, and will enhance the detection and identification of mRNA from target organisms.
authors.affiliation: Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch Straße 10, 35043, Marburg, Germany.
authors.forename: Marc G
authors.initials: MG
authors.lastname: Dumont
authors.forename: Bianca
authors.initials: B
authors.lastname: Pommerenke
authors.forename: Peter
authors.initials: P
authors.lastname: Casper
mesh_terms: D000332:Aerobiosis; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002247:Carbon Isotopes / Q000032:analysis / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology*; D060106:Lakes / Q000382:microbiology*; D054892:Metagenome; D008697:Methane / Q000032:analysis / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D059467:Transcriptome
chemical_list: D001426:Bacterial Proteins; D002247:Carbon Isotopes; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D008697:Methane