title: Proteome analysis of adenovirus using mass spectrometry.
abstract: Analysis of proteins and their posttranslational modifications is important for understanding different biological events. For analysis of viral proteomes, an optimal protocol includes production of a highly purified virus that can be investigated with a high-resolving analytical method. In this Methods in Molecular Biology paper we describe a working strategy for how structural proteins in the Adenovirus particle can be studied using liquid chromatography-high-resolving mass spectrometry. This method provides information on the chemical composition of the virus particle. Further, knowledge about amino acids carrying modifications that could be essential for any part of the virus life cycle is collected. We describe in detail alternatives available for preparation of virus for proteome analysis as well as choice of mass spectrometric instrumentation suitable for this kind of analysis.
authors.affiliation: Department of Immunology, Genetics, and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden.
authors.forename: Sara Bergström
authors.initials: SB
authors.lastname: Lind
authors.forename: Konstantin A
authors.initials: KA
authors.lastname: Artemenko
authors.forename: Ulf
authors.initials: U
authors.lastname: Pettersson
mesh_terms: D000256:Adenoviridae / Q000737:chemistry* / Q000302:isolation & purification; D018929:Cell Culture Techniques; D002853:Chromatography, Liquid / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D020543:Proteome / Q000737:chemistry*; D014771:Virion / Q000737:chemistry / Q000302:isolation & purification; D014779:Virus Replication
chemical_list: D020543:Proteome