title: Dehydroepiandrosterone-sulfate (DHEAS) promotes MIN6 cells insulin secretion via inhibition of AMP-activated protein kinase.
abstract: Derived from adrenal cortical, dehydroepiandrosterone-sulfate (DHEAS) is a precursor to androgens and estrogens, with various bioactivities. Although it has the property of anti-diabetes, the long-term effect of DHEAS on insulin secretion in beta-cells is still unclear. In this study, the effect of DHEAS on the insulin secretion activity in MIN6 cell lines in vitro was assessed. Insulin biosynthesis and secretion were stimulated by DHEAS for 24h. DHEAS inhibited the AMPK activation and upregulated the expression of ACC-1. These findings indicate that DHEAS may exert prominent stimulatory effects on insulin secretion partly via AMPK inhibition and ACC-1 upregulation.
authors.affiliation: Department of Metabolic and Endocrinology, Renji Hospital, Shanghai Jiaotong University School of Medicine, 160, Pujian Road, Pudong, Shanghai 200127, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Yue
authors.forename: Lihua
authors.initials: L
authors.lastname: Wang
authors.forename: Rong
authors.initials: R
authors.lastname: Huang
authors.forename: Shengxian
authors.initials: S
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Teng
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000123:Acetyltransferases / Q000096:biosynthesis / Q000235:genetics; D000620:Aminoimidazole Carboxamide / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D019314:Dehydroepiandrosterone Sulfate / Q000494:pharmacology*; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007328:Insulin / Q000096:biosynthesis / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D010766:Phosphorylation / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis; D012265:Ribonucleotides / Q000494:pharmacology
chemical_list: D007004:Hypoglycemic Agents; D007328:Insulin; D012333:RNA, Messenger; D012265:Ribonucleotides; D000620:Aminoimidazole Carboxamide; D019314:Dehydroepiandrosterone Sulfate; D000123:Acetyltransferases; C055210:aminoglycoside N1-acetyltransferase; D055372:AMP-Activated Protein Kinases; C031143:AICA ribonucleotide
keywords: AICA-riboside; AMP-activated protein kinase; Acetyl CoA carboxylase; Dehydroepiandrosterone-sulfate; Insulin secretion; MIN6 cells