title: [Preparation technology of total coumarins from Laportea bulbifera].
abstract: OBJECTIVE
  To investigate the preparation process of total coumarins from Laportea bulbifera.
  
  
  METHODS
  The optimum condition for the extraction of total coumarins was studied using the orthogonal test, and the extracts was purled with different kinds of macroporous resins. The content of total coumarins was detected by ultraviolet spectrophotometry.
  
  
  RESULTS
  The optimum extraction process was as follows: three-time circumfluence with 4 times volume of 75% ethanol for 1.5 h each time. HPD300 macroporous resin was the optimum one for the separation and purification of total coumarins. The extraction condition was loading 30 mL of 0.4 g/mL the crude drug, flowing at 2BV/h, then edulcorating with 4 times volume of distilled water and eluting with the same volume of 75% ethanol. The yield rate was more than 52%.
  
  
  CONCLUSIONS
  This method is suitable for the preparation of total coumarins in Laportea bulbifera, and the process is stable and repeatable.
authors.affiliation: School of Pharmacy,Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. tjphxm@yahoo.com.cn
authors.forename: Sha-Sha
authors.initials: SS
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.forename: Lu-Lu
authors.initials: LL
authors.lastname: Li
authors.forename: Jing-Li
authors.initials: JL
authors.lastname: Lu
authors.forename: Guo-Xiao
authors.initials: GX
authors.lastname: Yin
authors.forename: Ming
authors.initials: M
authors.lastname: Xiang
mesh_terms: D000327:Adsorption; D003374:Coumarins / Q000032:analysis / Q000302:isolation & purification*; D000431:Ethanol / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors; D027003:Urticaceae / Q000737:chemistry*
chemical_list: D003374:Coumarins; D012117:Resins, Synthetic; D000431:Ethanol