title: Decorin and biglycan immunolocalization in non-villous structures of healthy and pathological human placentas.
abstract: OBJECTIVE
  Decorin and biglycan are members of the small leucine-rich proteoglycan family, and constituents of both the extracellular matrix (ECM) and the cell surface. They are recognized as important factors in the control of proliferation, migration and invasion in vivo and in vitro. In this study, the localization patterns of decorin and biglycan were determined in healthy placentas and in highly invasive placental pathologies.
  
  
  RESULTS
  The study included immunolocalization of decorin and biglycan in samples of first-trimester and term placentas, placenta accreta, invasive mole, and choriocarcinoma. Extravillous cytotrophoblast (EVT) cells were positive for both proteoglycans in all pathologies and in first-trimester placentas, although not in term placentas. Biglycan was immunolocalized in the ECM of all healthy and pathological placentas, whereas decorin was observed only in term placenta ECM.
  
  
  CONCLUSIONS
  The expression of both proteoglycans was cell-specific and gestation time-dependent in healthy placentas, and was associated with invasive EVT cells in pathological placentas. In view of the biological properties of these molecules, it is possible that the biglycan pattern found here is intrinsically implicated in the invasive activity of EVT cells in both healthy and disordered placentas.
authors.affiliation: Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of Sao Paulo, São Paulo, Brazil.
authors.forename: Alexandre U
authors.initials: AU
authors.lastname: Borbely
authors.forename: Silvia
authors.initials: S
authors.lastname: Daher
authors.forename: Marcia M
authors.initials: MM
authors.lastname: Ishigai
authors.forename: Rosiane
authors.initials: R
authors.lastname: Mattar
authors.forename: Sue Y
authors.initials: SY
authors.lastname: Sun
authors.forename: Martin
authors.initials: M
authors.lastname: Knöfler
authors.forename: Estela
authors.initials: E
authors.lastname: Bevilacqua
authors.forename: Sergio F
authors.initials: SF
authors.lastname: Oliveira
mesh_terms: D058578:Biglycan / Q000378:metabolism*; D002822:Choriocarcinoma / Q000378:metabolism / Q000473:pathology; D058575:Decorin / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D006801:Humans; D002820:Hydatidiform Mole, Invasive / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D010920:Placenta / Q000033:anatomy & histology / Q000378:metabolism* / Q000473:pathology*; D010921:Placenta Accreta / Q000378:metabolism / Q000473:pathology; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000378:metabolism; D011263:Pregnancy Trimester, Third / Q000378:metabolism; D014594:Uterine Neoplasms / Q000378:metabolism / Q000473:pathology
chemical_list: C546884:BGN protein, human; D058578:Biglycan; C546878:DCN protein, human; D058575:Decorin
keywords: choriocarcinoma; immunohistochemistry; invasive mole; placenta accreta; trophoblast invasion