title: Multidrug-resistant human mycobacterium tuberculosis strain resisted inactivation by 10% formalin.
abstract: Multidrug-resistant strain of Mycobacterium tuberculosis (MDR-Tb) and Multidrug-sensitive Mycobacterium tuberculosis (MDS-Tb) isolated from humans were injected subcutaneously into the guinea pigs. One set of infected lungs of animals was fixed in 10% formalin pH7.2, while the other set was treated with 75% ethanol 2 hours prior to fixation in 10% formalin. After six months of fixation, MDS-Tb strains were completely inactivated by both methods of fixation. MDR-Tb strains resisted inactivation by 10% formalin, but were inactivated by the treatment with 75% ethanol 2 hours prior to 10% formalin fixation. MDR-Tb was still viable after six months in tissue preserved in 10% formalin only. Pretreatment of tissue with 75% ethanol prior to preservation in 10% formalin gave a better safety precaution against MDR-Tb in tissue. Of the 50 infected patients studied during the period, 4 (8%) haboured MDR-Tb, while 46 (92%) carried MDS-Tb.
authors.affiliation: Faculty of Basic Clinical Sciences, Bowen University Teaching Hospital, Ogbomoso, Nigeria.
authors.forename: A J
authors.initials: AJ
authors.lastname: Oke
authors.forename: A D
authors.initials: AD
authors.lastname: Adeyemo
authors.forename: A A
authors.initials: AA
authors.lastname: Oke
mesh_terms: D000818:Animals; D000431:Ethanol / Q000494:pharmacology*; D005557:Formaldehyde / Q000494:pharmacology*; D006168:Guinea Pigs; D006801:Humans; D008168:Lung / Q000382:microbiology; D050296:Microbial Viability / Q000187:drug effects*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000502:physiology; D013183:Sputum / Q000382:microbiology; D016707:Tissue Fixation / Q000379:methods; D018088:Tuberculosis, Multidrug-Resistant / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000382:microbiology
chemical_list: D005557:Formaldehyde; D000431:Ethanol