title: Structure and RNA-binding properties of the Not1-Not2-Not5 module of the yeast Ccr4-Not complex.
abstract: The Ccr4-Not complex is involved in several aspects of gene expression, including mRNA decay, translational repression and transcription. We determined the 2.8-Å-resolution crystal structure of a 120-kDa core complex of the Saccharomyces cerevisiae Not module comprising the C-terminal arm of Not1, Not2 and Not5. Not1 is a HEAT-repeat scaffold. Not2 and Not5 have extended regions that wrap around Not1 and around their globular domains, the Not boxes. The Not boxes resemble Sm folds and interact with each other with a noncanonical dimerization surface. Disruption of the interactions within the ternary complex has severe effects on growth in vivo. The ternary complex forms a composite surface that binds poly(U) RNA in vitro, with a site at the Not5 Not box. The results suggest that the Not module forms a versatile platform for macromolecular interactions.
authors.affiliation: Department of Structural Cell Biology, Max Planck Institute of Biochemistry (MPI Biochemistry), Martinsried, Germany.
authors.forename: Varun
authors.initials: V
authors.lastname: Bhaskar
authors.forename: Vladimir
authors.initials: V
authors.lastname: Roudko
authors.forename: Jérôme
authors.initials: J
authors.lastname: Basquin
authors.forename: Kundan
authors.initials: K
authors.lastname: Sharma
authors.forename: Henning
authors.initials: H
authors.lastname: Urlaub
authors.forename: Bertrand
authors.initials: B
authors.lastname: Séraphin
authors.forename: Elena
authors.initials: E
authors.lastname: Conti
mesh_terms: D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000378:metabolism*; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D012313:RNA / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D012097:Repressor Proteins / Q000737:chemistry* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000201:enzymology; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism*
chemical_list: C089931:CDC36 protein, S cerevisiae; C079592:CDC39 protein, S cerevisiae; D018797:Cell Cycle Proteins; C111175:NOT5 protein, S cerevisiae; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D012313:RNA