title: Abnormalities in cytokine secretion from mesenchymal stem cells in psoriatic skin lesions.
abstract: BACKGROUND
  Psoriasis is a chronic inflammatory and proliferative skin disease associated with immune abnormalities. In our preliminary studies, it was found that bone marrow mesenchymal stem cells (BMSCs) were abnormal in patients with psoriasis. Mesenchymal stem cells (MSCs) are not only present in the bone marrow, but can also be separated from the skin.We reasoned that an investigation into the biological characteristics of MSCs in psoriatic skin lesions could more realistically reflect the actual conditions for the pathogenesis of psoriasis.
  
  
  OBJECTIVE
  To reveal whether MSCs in psoriatic skin lesions are abnormal.
  
  
  METHODS
  We obtained MSCs from psoriatic skin lesions and healthy human skin and identified these cells using flow cytometry and a cell differentiation assay; cytokine concentrations in the culture supernatants were measured with the ELISA assay. We compared cytokine concentrations in culture supernatants of MSCs derived from psoriatic skin lesions as well as those from healthy human skin.
  
  
  RESULTS
  Compared with healthy control skintissue, in psoriatic skin lesions, MSC secretion of EGF, SCF, and IL-11 increased; secretion of bFGF, IL-3, IL-6, IL-8, and HGF decreased (p<0.05); and secretion of VEGF, M-CSF, G-CSF, GM-CSF, LIF, IL-1, IL-7, IL-10, and TNF-α showed no significant difference (p>0.05). Surface markers and the differentiation capacity of cells from the two sources were similar.
  
  
  CONCLUSIONS
  This study demonstrated that MSCs derived from psoriatic skin lesions had abnormalities, which may be one of the pathogenic mechanisms of psoriasis.
authors.affiliation: Institute of Dermatology, Taiyuan City Centre Hospital, Affiliated to Shanxi Medical University, No.1 Dong San Dao Xiang, Taiyuan, Shanxi Province 030009, China.
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Liu
authors.forename: Yuanwen
authors.initials: Y
authors.lastname: Yang
authors.forename: Xin
authors.initials: X
authors.lastname: Yan
authors.forename: Kaiming
authors.initials: K
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000378:metabolism; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D017370:Interleukin-11 / Q000378:metabolism; D007377:Interleukin-3 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D015851:Interleukin-7 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D053605:Leukemia Inhibitory Factor / Q000378:metabolism; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D011565:Psoriasis / Q000378:metabolism*; D012720:Severity of Illness Index; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D019089:Stem Cell Factor / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D055815:Young Adult
chemical_list: D016207:Cytokines; D007375:Interleukin-1; D017370:Interleukin-11; D007377:Interleukin-3; D015850:Interleukin-6; D015851:Interleukin-7; D016209:Interleukin-8; D053605:Leukemia Inhibitory Factor; D019089:Stem Cell Factor; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; D016753:Interleukin-10; D016179:Granulocyte Colony-Stimulating Factor; D004815:Epidermal Growth Factor; D017228:Hepatocyte Growth Factor; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor
keywords: cytokine secretion; mesenchymal stem cells; psoriasis; skin lesions