title: The behavior of ligament cells cultured on elastin and collagen scaffolds.
abstract: The ruptured anterior cruciate ligament (ACL) does not heal spontaneously. Therefore, the development of new healing techniques employing tissue engineering is vital. One of the aspects related to tissue-engineered artificial ligaments is the type of cell to be used for the artificial ligament. In this study, ligament cells from the ACL and periodontal ligament (PDL) were evaluated. In addition, we prepared highly oriented extracellular matrix (ECM) fiber scaffolds that mimicked the structure of the ligament and examined the cellular responses to these scaffolds. Elastin-A and collagen were used as the ECM proteins. Although the cells from the PDL (PDL fibroblasts [PDLFs]) showed approximately 2.1-fold higher expression of alkaline phosphatase (ALP; marker of osteogenic differentiation) than the ACL cells, the expression of ligament-related genes (for type I collagen, type III collagen, and tenomodulin) did not differ between PDLFs and ACL cells. Furthermore, the cellular responses (expression pattern of ligament-related genes and ALP activity) to the ECM were similar between ACL cells and PDLFs. In particular, elastin-A upregulated ALP and downregulated tenomodulin (TeM; a ligament marker) in ligament cells. In contrast, collagen maintained TeM expression in ligament cells. These results suggest that elastin-A promotes the osteogenic differentiation of ligament cells and that collagen maintains the phenotype of ligament cells.
authors.affiliation: Division of Chemistry for Materials, Faculty of Engineering, Graduate School of Mie University, 1577 Kurima-Machiyacho, Tsu, Mie, 514-8507, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Mizutani
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kageyama
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Yamada
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Keiichi
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Horiuchi
mesh_terms: D016118:Anterior Cruciate Ligament / Q000166:cytology* / Q000378:metabolism; D001842:Bone and Bones / Q000378:metabolism; D002478:Cells, Cultured; D003094:Collagen / Q000502:physiology*; D004549:Elastin / Q000502:physiology*; D005109:Extracellular Matrix / Q000502:physiology; D005347:Fibroblasts / Q000502:physiology; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D010513:Periodontal Ligament / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D003094:Collagen; D004549:Elastin