title: [Glucagon-like peptide-1 regulates lipometabolism by down-regulating adipose triglyceride lipase in 3T3-L1 adipocytes].
abstract: OBJECTIVE
  To investigate the effect of glucagon-like peptide-1 (GLP-1) on glycolipid metabolism in 3T3-L1 adipocytes and explore the mechanism.
  
  
  METHODS
  3T3-L1 adipocytes were treated with GLP-1, insulin, or both for 24 h, and Western blotting was used to analyze the expression levels of adipose triglyceride lipase (ATGL), glucose transporter type 4 (GLUT4), Akt1, Akt2 and phosphorylated Akt in the cells. Immunofluorescence was used to observe lipid content in 3T3-L1 adipocytes.
  
  
  RESULTS
  Akt1 and Akt2 were not activated by insulin stimulation in 3T3-L1 adipocytes. Akt was phosphorylated by GLP-1 stimulation, which inhibited the expression of ATGL and increased the translocation of GLUT4 from the intracellular membranes to plasma membranes. These changes were more obvious under the synergistic effect of insulin in 3T3-L1 adipocytes.
  
  
  CONCLUSIONS
  GLP-1 decreases lipolysis by inhibiting the expression of ATGL and improves insulin resistance by increasing the translocation of GLUT4 in 3T3-L1 adipocytes.
authors.affiliation: First Affiliated Hospital of Jinan University, Guangzhou 510630, China. E-mail: zhlffff@163.com.
authors.forename: Huili
authors.initials: H
authors.lastname: Zhu
authors.forename: Zeping
authors.initials: Z
authors.lastname: Weng
authors.forename: Chenli
authors.initials: C
authors.lastname: Lin
authors.forename: Jiwei
authors.initials: J
authors.lastname: Ma
authors.forename: Xueyun
authors.initials: X
authors.lastname: Zhong
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D015536:Down-Regulation; D004357:Drug Synergism; D052216:Glucagon-Like Peptide 1 / Q000494:pharmacology*; D051275:Glucose Transporter Type 4 / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D007333:Insulin Resistance; D007425:Intracellular Membranes / Q000378:metabolism; D008049:Lipase / Q000378:metabolism*; D051379:Mice; D010766:Phosphorylation; D021381:Protein Transport / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D000217:Acyltransferases
chemical_list: D052216:Glucagon-Like Peptide 1; D051275:Glucose Transporter Type 4; D007328:Insulin; D008049:Lipase; D051057:Proto-Oncogene Proteins c-akt; C495693:Slc2a4 protein, mouse; C514862:Akt1 protein, mouse; C494922:Akt2 protein, mouse; C494445:PNPLA2 protein, mouse; D000217:Acyltransferases