title: Biosynthetic support based on dendritic poly(L-lysine) improves human skin fibroblasts attachment.
abstract: Poly(L-lysine) (PLL) dendrigrafts (DGLs) are arborescent biosynthetic polymers of regular and controlled structures. They have specific properties such as biocompatibility and non-immunogenicity, and their surface density of NH2 functions can be easily modified and therefore appears as a powerful tool for the functionalization of hydrophobic polymers used in the context of tissue engineering. In this study, we evaluated several criteria of human skin fibroblasts when cultured with DGL of generations 2, 3 and 4, with linear PLL polymer as reference. In aqueous phase, DGLs and PLL displayed a similar cytotoxicity towards fibroblasts. Plastic culture plates grafted with DGLs were further characterized as homogeneous surfaces by atomic force microscopy and surface characterization by amino density estimation by colorimetric assay. Proliferation of fibroblasts was increased when cultured onto PLL and DGLs monolayers when compared with crude plates. Cellular adhesion was increased by 20% on DGLs in comparison to PLL. Integrin α5 subunit protein expression level was increased after 48 h of culture on DGLs, in comparison to control or PLL-coated surfaces. The presence of DGLs did not lead to overexpression or activation of matrix metalloproteinases 2 and 9. Finally, fibroblasts adhesion was increased by 40% on poly-(lactic-co-glycolic acid) matrices functionalized with DGLs when compared to PLL. Overall, these features make DGL promising candidates for the surface engineering of biomaterials in tissue engineering.
authors.affiliation: a LBTI, UMR5305 CNRS Université Lyon 1 , 69367 , Lyon Cedex 7 , France .
authors.forename: Chloé
authors.initials: C
authors.lastname: Lorion
authors.forename: Clément
authors.initials: C
authors.lastname: Faye
authors.forename: Barbara
authors.initials: B
authors.lastname: Maret
authors.forename: Thomas
authors.initials: T
authors.lastname: Trimaille
authors.forename: Thomas
authors.initials: T
authors.lastname: Régnier
authors.forename: Pascal
authors.initials: P
authors.lastname: Sommer
authors.forename: Romain
authors.initials: R
authors.lastname: Debret
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003712:Dendrites / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D011107:Polylysine / Q000737:chemistry*; D012867:Skin / Q000166:cytology*
chemical_list: D001672:Biocompatible Materials; D011107:Polylysine