title: Monoamines tissue content analysis reveals restricted and site-specific correlations in brain regions involved in cognition.
abstract: The dopamine (DA), noradrenalin (NA) and serotonin (5-HT) monoaminergic systems are deeply involved in cognitive processes via their influence on cortical and subcortical regions. The widespread distribution of these monoaminergic networks is one of the main difficulties in analyzing their functions and interactions. To address this complexity, we assessed whether inter-individual differences in monoamine tissue contents of various brain areas could provide information about their functional relationships. We used a sensitive biochemical approach to map endogenous monoamine tissue content in 20 rat brain areas involved in cognition, including 10 cortical areas and examined correlations within and between the monoaminergic systems. Whereas DA content and its respective metabolite largely varied across brain regions, the NA and 5-HT contents were relatively homogenous. As expected, the tissue content varied among individuals. Our analyses revealed a few specific relationships (10%) between the tissue content of each monoamine in paired brain regions and even between monoamines in paired brain regions. The tissue contents of NA, 5-HT and DA were inter-correlated with a high incidence when looking at a specific brain region. Most correlations found between cortical areas were positive while some cortico-subcortical relationships regarding the DA, NA and 5-HT tissue contents were negative, in particular for DA content. In conclusion, this work provides a useful database of the monoamine tissue content in numerous brain regions. It suggests that the regulation of these neuromodulatory systems is achieved mainly at the terminals, and that each of these systems contributes to the regulation of the other two.
authors.affiliation: Université de Bordeaux, F-33000 Bordeaux, France; CNRS, Institut des Neurosciences Cognitives et Intégratives d'Aquitaine, UMR 5287, F-33000 Bordeaux, France.
authors.forename: A
authors.initials: A
authors.lastname: Fitoussi
authors.forename: F
authors.initials: F
authors.lastname: Dellu-Hagedorn
authors.forename: P
authors.initials: P
authors.lastname: De Deurwaerdère
mesh_terms: D000818:Animals; D015306:Biogenic Monoamines / Q000032:analysis*; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D003071:Cognition / Q000502:physiology*; D055664:Electrochemical Techniques; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D015306:Biogenic Monoamines
keywords: 3,4-dihydroxyphenylacetic acid; 5-HIAA; 5-HT; 5-hydroxyindole-3-acetic acid; 5-hydroxytryptamine (serotonin); BLA; CE; DA; DLO; DLS; DMS; DOPAC; DRN; EDTA; HPC; HPLC; IL; LC; LO; M2; MO; MRN; NA; OFC; PL; SNc; STN; VLS; VMS; VTA; aCg; aIns; anterior cingulate cortex; anterior insular cortex; basolateral nucleus of the amygdala; central nucleus of the amygdala; cognition; core; core of the nucleus accumbens; dopamine; dorsal raphe nucleus; dorsolateral orbitofrontal cortex; dorsolateral striatum; dorsomedial striatum; ethylenediaminetetraacetic acid; high pressure liquid chromatography; hippocampus; infralimbic cortex; lateral orbitofrontal cortex; locus coeruleus; medial orbitofrontal cortex; median raphe nucleus; monoamine tissue content; motor cortex; noradrenalin; orbitofrontal cortices; pCg; pIns; posterior cingulate cortex; posterior insular cortex; prelimbic cortex; rat; serotonin; shell; shell of the nucleus accumbens; substantia nigra pars compacta; subthalamic nucleus; ventral tegmental area; ventrolateral striatum; ventromedial striatum