title: Detection and characterization of 1,2-dibromoethane-derived DNA crosslinks formed with O(6) -alkylguanine-DNA alkyltransferase.
abstract: A combination of chemical modifications and LC-tandem MS was used for the structure elucidation of various ethylene crosslinks of DNA with O(6) -alkylguanine-DNA alkyltransferase (AGT, see picture). The elucidation of the chemical structures of such DNA-protein crosslinks is necessary to understand mechanisms of mutagenesis.
authors.affiliation: Department of Biochemistry, Vanderbilt University School of Medicine, 638 RRB, 2220 Pierce Ave., Nashville, TN 37232 (USA).
authors.forename: Goutam
authors.initials: G
authors.lastname: Chowdhury
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Cho
authors.forename: Anthony E
authors.initials: AE
authors.lastname: Pegg
authors.forename: F Peter
authors.initials: FP
authors.lastname: Guengerich
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D018736:DNA Adducts; D004249:DNA Damage; D006801:Humans; D006865:Hydrogenation; D009154:Mutation; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D018736:DNA Adducts; D004247:DNA; D019853:O(6)-Methylguanine-DNA Methyltransferase
keywords: DNA adducts; DNA damage; DNA-protein crosslinks; hydrogenation; mutations