title: Development and application of a LC-MS/MS assay for the simultaneous quantification of edaravone and taurine in beagle plasma.
abstract: An LC-MS/MS method was developed and validated for the simultaneous quantification of edaravone and taurine in beagle plasma. The plasma sample was deproteinized using acetonitrile containing formic acid. Chromatographic separations were achieved on an Agilent Zorbax SB-Aq (100 × 2.1 mm, 3.5 μm) column, with a gradient of water (containing 0.03% formic acid) and methanol as the mobile phase at a flow rate of 0.3 mL/min. The analyte detection was carried out in multiple reaction monitoring mode and the optimized precursor-to-product transitions of m/z [M+H](+) 175.1 → 133.0 (edaravone), m/z [M+H](+) 189.1 → 147.0 (3-methyl-1-p-tolyl-5-pyrazolone, internal standard, IS), m/z [M-H](-) 124.1→80.0 (taurine), and m/z [M-H](-) 172.0 → 80.0 (sulfanilic acid, IS) were employed to quantify edaravone, taurine, and their corresponding ISs, respectively. The LOD and the lower LOQ were 0.01 and 0.05 μg/mL for edaravone and 0.66 and 2 μg/mL for taurine, respectively. The calibration curves of these two analytes demonstrated good linearity (r ＞ 0.99). All the validation data including the specificity, precision, recovery, and stability conformed to the acceptable requirements. This validated method has successfully been applied in the pharmacokinetic study of edaravone and taurine mixture in beagle dogs.
authors.affiliation: Department of Pharmaceutical Analysis, Xuzhou Medical College, Xuzhou, Jiangsu, China; Key Laboratory of New Drug and Clinical Application, Xuzhou Medical College, Xuzhou, Jiangsu, China.
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: Yu
authors.forename: Xiao-xiao
authors.initials: XX
authors.lastname: Zheng
authors.forename: Ting-ting
authors.initials: TT
authors.lastname: Bian
authors.forename: Yin-jie
authors.initials: YJ
authors.lastname: Li
authors.forename: Xiao-wen
authors.initials: XW
authors.lastname: Wu
authors.forename: Dong-zhi
authors.initials: DZ
authors.lastname: Yang
authors.forename: Shui-shi
authors.initials: SS
authors.lastname: Jiang
authors.forename: Dao-quan
authors.initials: DQ
authors.lastname: Tang
mesh_terms: D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D000077553:Edaravone; D053719:Tandem Mass Spectrometry; D013654:Taurine / Q000097:blood* / Q000737:chemistry
chemical_list: D013654:Taurine; D000077553:Edaravone; D000983:Antipyrine
keywords: Edaravone; LC-MS/MS; Pharmacokinetics; Taurine