title: Development of a rapid LC-DAD/FLD method for the simultaneous determination of auxins and abscisic acid in plant extracts.
abstract: Plant hormones play a crucial role in controlling plant growth and development. These groups of naturally occurring substances trigger physiological processes at very low concentrations, which mandate sensitive techniques for their quantitation. This paper describes a method to quantify endogenous (±)-2-cis-4-trans-abscisic acid, indole-3-acetic acid, indole-3-propionic acid, and indole-3-butyric acid. The method combines high-performance liquid chromatography (HPLC) with diode array and fluorescence detection in a single run. Hybrid tea rose 'Monferrato' matrices (leaves, petals, roots, seeds, androecium, gynoecium, and pollen) were used as references. Rose samples were separated and suspended in extracting methanol, after which (±)-2-cis-4-trans-abscisic acid and auxins were extracted by solvent extraction. Sample solutions were added first to cation solid phase extraction (SPE) cartridges and the eluates to anion SPE cartridges. The acidic hormones were bound to the last column and eluted with 5% phosphoric acid in methanol. Experimental results showed that this approach can be successfully applied to real samples and that sample preparation and total time for routine analysis can be greatly reduced.
authors.affiliation: Department of Agricultural, Forest and Food Sciences, University of Torino , Via Leonardo Da Vinci 44, 10095 Grugliasco (To), Italy.
authors.forename: Renato
authors.initials: R
authors.lastname: Bosco
authors.forename: Matteo
authors.initials: M
authors.lastname: Caser
authors.forename: Francesca
authors.initials: F
authors.lastname: Vanara
authors.forename: Valentina
authors.initials: V
authors.lastname: Scariot
mesh_terms: D000040:Abscisic Acid / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D007210:Indoleacetic Acids / Q000032:analysis* / Q000302:isolation & purification; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification; D010937:Plant Growth Regulators / Q000032:analysis* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D027826:Rosa / Q000737:chemistry*
chemical_list: D007210:Indoleacetic Acids; D010936:Plant Extracts; D010937:Plant Growth Regulators; D000040:Abscisic Acid