title: Enrichment of Bifidobacterium longum subsp. infantis ATCC 15697 within the human gut microbiota using alginate-poly-L-lysine-alginate microencapsulation oral delivery system: an in vitro analysis using a computer-controlled dynamic human gastrointestinal model.
abstract: This study evaluates alginate-poly-L-lysine-alginate Bifidobacterium longum subsp. infantis ATCC 15697-loaded microcapsules to enrich the human gut microbiota. The cell survival of alginate-poly-L-lysine-alginate microencapsulated B. infantis ATCC 15697 in gastric acid, bile, and through human gastrointestinal transit was investigated, as well as the formulation's effect on the gut microbiota. Results show that microencapsulation increases B. infantis ATCC 15697 cell survival at pH1.0 (33.54 ± 2.80% versus <1.00 ± 0.00%), pH1.5 (41.15 ± 2.06% versus <1.00 ± 0.00%), pH2.0 (60.88 ± 1.73% versus 36.01 ± 2.63%), pH3.0 (75.43 ± 1.23% versus 46.30 ± 1.43%), pH4.0 (71.40 ± 2.02% versus 47.75 ± 3.12%) and pH5.0 (73.88 ± 3.79% versus 58.93 ± 2.26%) (p < 0.05). In addition, microencapsulation increases cell survival at 0.5% (76.85 ± 0.80% versus 70.77 ± 0.64%), 1.0% (59.99 ± 0.97% versus 53.47 ± 0.58%) and 2.0% (53.10 ± 1.87% versus 44.59 ± 1.52%) (p < 0.05) (w/v) bile. Finally, daily administration of alginate-poly-L-lysine-alginate microencapsulated B. infantis ATCC 15697 in a human gastrointestinal model induces a significant enrichment of B. infantis within the ascending (184.51 ± 17.30% versus 53.83 ± 17.82%; p < 0.05), transverse (174.79 ± 25.32% versus 73.17 ± 15.30%; p < 0.05) and descending (94.90 ± 25.22% versus 46.37 ± 18.93%; p > 0.05) colonic microbiota.
authors.affiliation: Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, Artificial Cells and Organs Research Centre, McGill University , Montreal, Quebec , Canada .
authors.forename: Laetitia
authors.initials: L
authors.lastname: Rodes
authors.forename: Catherine
authors.initials: C
authors.lastname: Tomaro-Duchesneau
authors.forename: Shyamali
authors.initials: S
authors.lastname: Saha
authors.forename: Arghya
authors.initials: A
authors.lastname: Paul
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Malhotra
authors.forename: Daniel
authors.initials: D
authors.lastname: Marinescu
authors.forename: Wei
authors.initials: W
authors.lastname: Shao
authors.forename: Imen
authors.initials: I
authors.lastname: Kahouli
authors.forename: Satya
authors.initials: S
authors.lastname: Prakash
mesh_terms: D000284:Administration, Oral; D000464:Alginates / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D001644:Bifidobacterium; D002214:Capsules; D003198:Computer Simulation; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D064307:Microbiota; D008954:Models, Biological; D011107:Polylysine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D013270:Stomach / Q000382:microbiology*
chemical_list: D000464:Alginates; D002214:Capsules; C051546:alginate-polylysine-alginate; D011107:Polylysine