title: Gamete quality of streaked prochilod Prochilodus lineatus (Characiformes) after GnRHa and dopamine antagonist treatment.
abstract: The efficiency of Ovaprim™ salmon gonadotropin-releasing hormone agonist (GnRHa) and dopamine antagonist on the induction of spawning and spermiation in Prochilodus lineatus in comparison with the commonly used method using pituitary extract (PE) was evaluated. Females received PE at 0.5 + 5.0 mg/kg and Ovaprim™ at 0.05 + 0.45 ml/kg or at 0.125 + 0.375 ml/kg. All males received a first dose of PE at 0.4 mg/kg and then PE at 4.0 mg/kg or Ovaprim™ at 0.25 ml/kg. Oocyte, egg, larvae and sperm quality were evaluated. All females spawned and oocyte, egg and larvae quality were similar between Ovaprim™-treated (both doses) and PE-treated females. Data from females were pooled and the mean values were: 242 g ova weight, 15% ova index, 1209 oocytes/g ova, 284,539 oocytes/female, 183 oocytes/g body weight, 1.18 mm oocyte diameter, 49% fertilization rate, 43% hatching rate and 89% normal larvae. Sperm quality was similar between Ovaprim™-treated and PE-treated males. Data from males were pooled and the mean values of semen were: volume of 3.0 ml, 14.9 × 109 sperm/ml, osmolality of 283 mOsm/kg, pH of 7.4, 71% motile sperm, 217 μm/s curvilinear velocity, 102 μm/s straight-line velocity and 189 μm/s average path velocity. Ovaprim™ treatment can be used for commercial reproduction of P. lineatus, without any loss of gamete quality in comparison with PE treatment.
authors.affiliation: Department of Animal Science (DZO),Federal University of Lavras,(UFLA),PO Box 3037,Lavras,MG,37200-000,Brazil.
authors.forename: Ana T M
authors.initials: AT
authors.lastname: Viveiros
authors.affiliation: Department of Animal Science,Federal University of Lavras (UFLA),P.O. Box 3037,Lavras,Minas Gerais,37200-000,Brazil.
authors.forename: Antônio C S
authors.initials: AC
authors.lastname: Gonçalves
authors.affiliation: Department of Animal Science,Federal University of Lavras (UFLA),P.O. Box 3037,Lavras,Minas Gerais,37200-000,Brazil.
authors.forename: Isabela M
authors.initials: IM
authors.lastname: Di Chiacchio
authors.affiliation: Department of Animal Science,Federal University of Lavras (UFLA),P.O. Box 3037,Lavras,Minas Gerais,37200-000,Brazil.
authors.forename: Ariane F
authors.initials: AF
authors.lastname: Nascimento
authors.affiliation: Institute of Fisheries,APTA,SAA,SP,Brazil.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Romagosa
authors.affiliation: Department of Animal Science,Federal University of Lavras (UFLA),P.O. Box 3037,Lavras,Minas Gerais,37200-000,Brazil.
authors.forename: Marcelo C
authors.initials: MC
authors.lastname: Leal
mesh_terms: D000818:Animals; D017756:Aquaculture / Q000379:methods; D060125:Characiformes / Q000502:physiology*; D004294:Domperidone / Q000494:pharmacology*; D018492:Dopamine Antagonists / Q000494:pharmacology*; D004338:Drug Combinations; D005260:Female; D005306:Fertilization / Q000187:drug effects; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists* / Q000494:pharmacology; D007814:Larva / Q000187:drug effects; D008297:Male; D009865:Oocytes / Q000187:drug effects; D010902:Pituitary Gland / Q000737:chemistry; D012098:Reproduction / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects; D014020:Tissue Extracts / Q000494:pharmacology
chemical_list: D018492:Dopamine Antagonists; D004338:Drug Combinations; D014020:Tissue Extracts; C527878:ovaprim; D007987:Gonadotropin-Releasing Hormone; D004294:Domperidone