title: MLVA genotyping of Brucella melitensis and Brucella abortus isolates from different animal species and humans and identification of Brucella suis vaccine strain S2 from cattle in China.
abstract: In China, brucellosis is an endemic disease and the main sources of brucellosis in animals and humans are infected sheep, cattle and swine. Brucella melitensis (biovars 1 and 3) is the predominant species, associated with sporadic cases and outbreak in humans. Isolates of B. abortus, primarily biovars 1 and 3, and B. suis biovars 1 and 3 are also associated with sporadic human brucellosis. In this study, the genetic profiles of B. melitensis and B. abortus isolates from humans and animals were analyzed and compared by multi-locus variable-number tandem-repeat analysis (MLVA). Among the B. melitensis isolates, the majority (74/82) belonged to MLVA8 genotype 42, clustering in the 'East Mediterranean' group. Two B. melitensis biovar 1 genotype 47 isolates, belonging to the 'Americas' group, were recovered; both were from the Himalayan blue sheep (Pseudois nayaur, a wild animal). The majority of B. abortus isolates (51/70) were biovar 3, genotype 36. Ten B. suis biovar 1 field isolates, including seven outbreak isolates recovered from a cattle farm in Inner Mongolia, were genetically indistinguishable from the vaccine strain S2, based on MLVA cluster analysis. MLVA analysis provided important information for epidemiological trace-back. To the best of our knowledge, this is the first report to associate Brucella cross-infection with the vaccine strain S2 based on molecular comparison of recovered isolates to the vaccine strain. MLVA typing could be an essential assay to improve brucellosis surveillance and control programs.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, Beijing, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Jiang
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.forename: Liqing
authors.initials: L
authors.lastname: Xu
authors.forename: Guiying
authors.initials: G
authors.lastname: Hu
authors.forename: Junying
authors.initials: J
authors.lastname: Ma
authors.forename: Pei
authors.initials: P
authors.lastname: Xiao
authors.forename: Weixing
authors.initials: W
authors.lastname: Fan
authors.forename: Dongdong
authors.initials: D
authors.lastname: Di
authors.forename: Guozhong
authors.initials: G
authors.lastname: Tian
authors.forename: Mengguang
authors.initials: M
authors.lastname: Fan
authors.forename: Jingchuan
authors.initials: J
authors.lastname: Mi
authors.forename: Ruiping
authors.initials: R
authors.lastname: Yu
authors.forename: Litao
authors.initials: L
authors.lastname: Song
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhao
authors.forename: Dongri
authors.initials: D
authors.lastname: Piao
authors.forename: Buyun
authors.initials: B
authors.lastname: Cui
mesh_terms: D000818:Animals; D002004:Brucella Vaccine; D002003:Brucella abortus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017347:Brucella melitensis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D041021:Brucella suis / Q000235:genetics* / Q000276:immunology; D002006:Brucellosis / Q000453:epidemiology* / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology*; D002681:China; D006801:Humans; D058885:Multilocus Sequence Typing; D010802:Phylogeny
chemical_list: D002004:Brucella Vaccine