title: Wickerhamomyces sylviae f.a., sp. nov., an ascomycetous yeast species isolated from migratory birds.
abstract: In the present work, we investigated the phylogenetic position and phenotypic characteristics of eight yeast isolates collected from migratory birds on the island of Ustica, Italy. A phylogenetic analysis based on the D1/D2 region of the large-subunit rRNA gene showed that all isolates clustered as a single separate lineage within the Wickerhamomyces clade. They exhibited distinct morphological and physiological characteristics and were clearly separated from their closest relatives, Wickerhamomyces lynferdii, Wickerhamomyces anomalus and Wickerhamomyces subpelliculosus, in blastn searches. On the basis of the isolation source, physiological features and molecular strain typing carried out with randomly amplified polymorphic DNA (RAPD)-PCR and minisatellite-primed (MSP)-PCR analysis, the isolates were identified as strains of the same species. The name Wickerhamomyces sylviae f.a., sp. nov. is proposed to accommodate these novel strains; the type strain is U88A2(T) ( = PYCC 6345(T) = CBS 12888(T)). The MycoBank number is MB 804762.
authors.affiliation: Centro de Recursos Microbiológicos, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.|Department of Agricultural and Forest Science, Università degli Studi di Palermo, Viale delle Scienze 4, 90128 Palermo, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Francesca
authors.affiliation: Centro de Recursos Microbiológicos, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Cláudia
authors.initials: C
authors.lastname: Carvalho
authors.affiliation: Centro de Recursos Microbiológicos, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Pedro Miguel
authors.initials: PM
authors.lastname: Almeida
authors.affiliation: Department of Agricultural and Forest Science, Università degli Studi di Palermo, Viale delle Scienze 4, 90128 Palermo, Italy.
authors.forename: Ciro
authors.initials: C
authors.lastname: Sannino
authors.affiliation: Department of Agricultural and Forest Science, Università degli Studi di Palermo, Viale delle Scienze 4, 90128 Palermo, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Settanni
authors.affiliation: Centro de Recursos Microbiológicos, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: José Paulo
authors.initials: JP
authors.lastname: Sampaio
authors.affiliation: Department of Agricultural and Forest Science, Università degli Studi di Palermo, Viale delle Scienze 4, 90128 Palermo, Italy.
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Moschetti
mesh_terms: D025041:Animal Migration; D000818:Animals; D001717:Birds / Q000382:microbiology*; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000235:genetics; D062312:Islands; D007558:Italy; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique; D054683:Ribosome Subunits, Large, Eukaryotic / Q000235:genetics; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal