title: Metabolite profile of marine-derived endophytic Streptomyces sundarbansensis WR1L1S8 by liquid chromatography-mass spectrometry and evaluation of culture conditions on antibacterial activity and mycelial growth.
abstract: OBJECTIVE
  This study was designed to investigate whether culture conditions (media, seawater concentration and pH) could lead Streptomyces sundarbansensis strain (isolated from marine brown algae Fucus sp. collected from Algerian coastline) to produce bioactive secondary metabolites. The most favourable condition for the production of anti-MRSA compound 1 [2-hydroxy-5-((6-hydroxy-4-oxo-4H-pyran-2-yl)methyl)-2-propylchroman-4-one] was determined.
  
  
  RESULTS
  The profile of metabolites present in the crude extracts was carried out by HPLC analysis equipped with a diode array detector evaporative light scattering detection (DAD-ELSD) or online coupled to electrospray ionization-mass spectrometry (ESI-MS). Compound 1 was the most abundant secondary metabolite by culturing the strains on starch casein agar (SCA) medium in freshwater or 50% seawater at pH 7 or 9 using agar-state fermentation method.
  
  
  CONCLUSIONS
  The study has shown the efficiency of HPLC/ESI-MS technique in the analysis of polyketides produced by the strain under investigation. It was possible to establish the best culture conditions for obtaining the most bioactive compound 1, previously isolated by the same strain.
  
  
  CONCLUSIONS
  Marine algae-actinobacteria associations are a particularly promising renewable system for the production of new antibacterial metabolites. Based on the promising bioactivity of the chemically characterized compound 1, the analytical methodology here applied has resulted as an effective approach for establishing its optimized production.
authors.affiliation: Bioorganic Chemistry Laboratory, Department of Physics, University of Trento, Povo, Italy; Laboratory of Applied Microbiology, Faculty of Nature Science and Life, University of Bejaia, Targa Ouzemmour, Bejaia, Algeria.
authors.forename: I
authors.initials: I
authors.lastname: Djinni
authors.forename: A
authors.initials: A
authors.lastname: Defant
authors.forename: M
authors.initials: M
authors.lastname: Kecha
authors.forename: I
authors.initials: I
authors.lastname: Mancini
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D003470:Culture Media / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D013302:Streptomyces / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media
keywords: LC-MS analysis; actinomycetes; algae; antimicrobials; polyketide