title: Cytotoxic effects of Reactive Blue 33 on Allium cepa determined using Taguchi's L₈ orthogonal array.
abstract: In this study, Taguchi L₈ experimental design was applied to determine cytotoxic effects of Reactive Blue 33, which is the most toxic azo reactive dye species, on Allium cepa. With this aim, A. cepa test system was performed to achieve targeted experimental design with three factors (concentration of dye, pH and volume) in two different levels. Toxic conditions were determined considering calculated signal-to-noise ratios. "Smaller is better" approach was followed to calculate signal-to-noise ratios as it was aimed to obtain lower root lengths. In the work, toxic effects of azo dye were also predicted by using the Taguchi method. Taguchi model showed that experimental and predicted values were closer to each other demonstrating the success of Taguchi approach.
authors.affiliation: Kocaeli University, Department of Biology, 41380 Kocaeli, Turkey.
authors.forename: Gonca
authors.initials: G
authors.lastname: Al
authors.forename: Utkan
authors.initials: U
authors.lastname: Özdemir
authors.forename: Özlem
authors.initials: Ö
authors.lastname: Aksoy
mesh_terms: D001391:Azo Compounds / Q000633:toxicity*; D004396:Coloring Agents / Q000633:toxicity*; D003603:Cytotoxins / Q000633:toxicity*; D004785:Environmental Pollutants / Q000633:toxicity*; D006863:Hydrogen-Ion Concentration; D019697:Onions / Q000187:drug effects* / Q000254:growth & development; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D003603:Cytotoxins; D004785:Environmental Pollutants; C587226:Reactive Blue 33
keywords: Allium cepa; Cytotoxic effects; Reactive azo dye; Root growth; Signal-to-noise; Taguchi