title: Increased stability and protease resistance of the β-lactoglobulin/vitamin D3 complex.
abstract: The stability of the β-lactoglobulin (βlg)/vitamin D3 (D3) complex at 4°C and upon exposure to UV-C light, and in simulated intestinal fluid, were studied in vitro. Caco-2 cells were used to demonstrate the passage of the βlg/D3 complex across the monolayers. Furthermore, an in vivo experiment was conducted by force-feeding rats with the free D3 and βlg/D3 complex, with subsequent determination of the plasma concentration of 25-hydroxy-D3. The βlg/D3 complex significantly improved the stability of the vitamin at 4 °C and when exposed to UV-C light. The resistance of βlg to proteases was increased, indicating a mutual protective effect. The βlg/D3 complex crossed the monolayers, which was confirmed by the significant increase in the concentration of 25-hydroxy-D3 in rats fed the βlg/D3 complex compared to the ones fed the free D3. Therefore, the current study suggests that the βlg/D3 complex can effectively be used for the fortification of milk products and low-fat content foods to improve the intake and bioavailability of D3.
authors.affiliation: Chaire de recherche du Canada sur les protéines, les bio-systèmes et les aliments fonctionnels, Institut de recherche sur les nutraceutiques et les aliments fonctionnels (INAF/STELA), Université Laval, Québec, QC, Canada.
authors.forename: Fatoumata
authors.initials: F
authors.lastname: Diarrassouba
authors.forename: Ghislain
authors.initials: G
authors.lastname: Garrait
authors.forename: Gabriel
authors.initials: G
authors.lastname: Remondetto
authors.forename: Pedro
authors.initials: P
authors.lastname: Alvarez
authors.forename: Eric
authors.initials: E
authors.lastname: Beyssac
authors.forename: Muriel
authors.initials: M
authors.lastname: Subirade
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D002463:Cell Membrane Permeability; D002762:Cholecalciferol / Q000097:blood / Q000737:chemistry*; D004355:Drug Stability; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007782:Lactoglobulins / Q000737:chemistry*; D008297:Male; D010447:Peptide Hydrolases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013696:Temperature; D014466:Ultraviolet Rays
chemical_list: D007782:Lactoglobulins; D002762:Cholecalciferol; D010447:Peptide Hydrolases
keywords: Bioavailability; Complex; Permeability; Stability; Vitamin D(3); β-Lactoglobulin