title: Cell-free preparation of functional and triggerable giant proteoliposomes.
abstract: Heat, we leak: We express a membrane protein outside well-defined giant liposomes obtained by gravity-transferred sucrose-in-oil droplets into a cell-free, reconstituted expression system. We show that the presence of the liposome is necessary during expression for efficient protein insertion into the membrane and that temperature can trigger the resulting membrane function.
authors.affiliation: Department of Chemistry, Ecole Normale Superieure, 24 rue Lhomond, 75005 Paris (France) http://www.baigllab.com/; Université Pierre et Marie Curie Paris 6, 4 place Jussieu, 75005 Paris (France); UMR 8640, CNRS, 3 rue Michel-Ange, 75016 Paris (France).
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Liu
authors.forename: Gregory P R
authors.initials: GP
authors.lastname: Hansen
authors.forename: Anna
authors.initials: A
authors.lastname: Venancio-Marques
authors.forename: Damien
authors.initials: D
authors.lastname: Baigl
mesh_terms: D002474:Cell-Free System; D008081:Liposomes / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D010316:Particle Size; D011510:Proteolipids / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D013395:Sucrose; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors
chemical_list: D008081:Liposomes; D008565:Membrane Proteins; D011510:Proteolipids; C020485:proteoliposomes; D013395:Sucrose
keywords: GUV; artificial cells; connexin; liposomes; membrane proteins