title: Solid-phase PEGylation of an immobilized protein cage on polyelectrolyte multilayer.
abstract: We used a quartz crystal microbalance (QCM) to quantitatively characterize solid-phase poly(ethylene glycol) modification (PEGylation) of apoferritin that was electrostatically immobilized on the surface of a polyelectrolyte multilayer. The solid-phase PEGylation processes were monitored by analyzing QCM frequency shifts, which showed that the PEG chains were covalently introduced onto the surface of the immobilized apoferritin. We investigated the effect of PEG concentration, PEG molecular weight, and two-dimensional coverage of the immobilized apoferritin on the solid-phase PEGylation process in addition to the surface properties of the PEGylated apoferritin film, such as wettability and protein adsorption capacity. Since the reaction field is more spatially restricted in solid-phase PEGylation than in traditional aqueous-phase PEGylation, this study shows that a ferritin protein cage is potentially useful as a tailored building block, one that has well-defined structures different from the PEGylated ferritin prepared by an aqueous-phase approach.
authors.affiliation: Department of Nanostructure and Advanced Materials, Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan; Biomaterials Unit, International Research Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
authors.forename: Koichiro
authors.initials: K
authors.lastname: Uto
authors.affiliation: Department of Nanostructure and Advanced Materials, Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Yamamoto
authors.affiliation: PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Iwahori
authors.affiliation: Department of Nanostructure and Advanced Materials, Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan; Biomaterials Unit, International Research Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. Electronic address: Aoyagi.Takao@nims.go.jp.
authors.forename: Takao
authors.initials: T
authors.lastname: Aoyagi
authors.affiliation: Nara Institute of Science and Technology, Materials Science, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan; CREST, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan; Advanced Technology Research Laboratories, Matsushita Electric Industrial Co. Ltd., Seika, Kyoto 619-0237, Japan. Electronic address: ichiro@ms.naist.jp.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Yamashita
mesh_terms: D001052:Apoferritins / Q000737:chemistry; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D011108:Polymers; D011092:Polyethylene Glycols; D001052:Apoferritins
keywords: Apoferritin; PEGylation; Polyelectrolyte multilayer; Protein cage; Quartz crystal microbalance; Solid-phase reaction