title: Effect of different anesthesia methods on erythrocyte immune function in mice.
abstract: OBJECTIVE
  To explore effect of different anesthesia methods and different anesthetics on erythrocyte immune function in mice.
  
  
  METHODS
  The mice were anesthetized by isoflurane and ether inhalation, and also under intraperitoneal anesthesia with sodium pentobarbital and chloral hydrate. Blood was collected from the ventro-cardinal vein. Automatic blood cell analyzer was used for routine blood examination, and the canthine oxidase method was used to measure the superoxide dismutase (SOD) activity. Lipid peroxidation product malondialdehyde (MDA) was measured with TBA, and glutathione peroxidase (GSH-Px) was measured with DTNB, and then the effect of different anesthesia methods and different anesthetics on erythrocyte immune function in mice was observed.
  
  
  RESULTS
  Hct level of chloral hydrate intraperitoneal injection group was significantly higher than the other three groups (P<0.05). And the MDA levels in the pentobarbital sodium group were significantly higher than the other three groups (P<0.05). SOD and GSH-Px of the chloral hydrate and sodium pentobarbital intraperitoneal injection group were significantly lower than the other two groups; RBC-C 3bRR and RBC-ICR of the chloral hydrate and sodium pentobarbital intraperitoneal injection group were significantly lower than the other two groups.
  
  
  CONCLUSIONS
  Different drugs can induce changes in immune function of mice at different levels. Isoflurane and ether have less damage to animal body, while chloral hydrate and sodium pentobarbital intraperitoneal injection have a certain inhibitory effect on the animal body respiratory system and can cause greater damage to the body. Therefore, the reasonable selection and control of anesthetics are very important in order to avoid the experimental errors caused by anesthesia.
authors.affiliation: Department of Anesthesiology, No 175Hospital of PLA, Affiliated Southeast Hospital of Xiamen University, Zhangzhou 363000, China.
authors.forename: Wen-Long
authors.initials: WL
authors.lastname: Zhang
authors.forename: Meng-Ying
authors.initials: MY
authors.lastname: Liu
authors.forename: Zhen-Chen
authors.initials: ZC
authors.lastname: Zhang
authors.forename: Chen-Yang
authors.initials: CY
authors.lastname: Duan
mesh_terms: D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D048430:Cell Shape / Q000187:drug effects; D004909:Erythrocyte Indices / Q000187:drug effects; D004912:Erythrocytes / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000032:analysis / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism; D051379:Mice; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism
chemical_list: D000777:Anesthetics; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Anesthesia; Chloral hydrate; Erythrocytes; Ether; Immune function; Isoflurane; Mice; Sodium pentobarbital