title: Selected isomiR expression profiles via arm switching?
abstract: A mature miRNA may be generated from 5p or 3p arm of a hairpin precursor. The selection may be flexible via "arm switching". However, accumulating evidences suggest that both arms of many pre-miRNAs can yield mature functional miRNAs. Herein, we attempted to compare the isomiR expression profiles between the two arms through analyzing in-house and published small RNA deep sequencing datasets. Although many miR-#-5p and miR-#-3p have been reported as functional miRNAs, fewer miRNA pairs (11 and 6 pairs are collected in tumor and normal cells, respectively) are simultaneously identified as abundant miRNA species. According to isomiR types and dominant isomiR species, miR-#-5p and miR-#-3p show various isomiR expression profiles as well as diverse enrichment levels. IsomiR profiles of non-dominant arm are not well-conserved in 5' ends as well as isomiR profiles of dominant arm. If both the miR-#-5p and miR-#-3p are abundantly expressed, their isomiR expression profiles are always stable across different samples. Similar to diverse enrichment levels of miR-#-5p and miR-#-3p, the isomiR expression patterns may also be influenced by the phenomenon of "arm switching". The diverged isomiR expression profiles further enrich the complexity of multiple isomiRs, and complicate the coding-non-coding RNA regulatory network.
authors.affiliation: Department of Epidemiology and Biostatistics and Ministry of Education Key Lab for Modern Toxicology, School of Public Health, Nanjing Medical University, 211166, China. Electronic address: gl8008@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Guo
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Sheng
authors.initials: S
authors.lastname: Yang
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
mesh_terms: D020869:Gene Expression Profiling; D035683:MicroRNAs / Q000235:genetics*
chemical_list: D035683:MicroRNAs
keywords: 3′ UTR; 3′-untranslated regions; Arm switching; Homo sapiens; IsomiR; IsomiR expression profile; KS; Kolmogorov–Smirnov; MicroRNA (miRNA); Mus musculus; RISC; RNA-induced silencing complex; Sus scrofa; hsa; miRNA; miRNA star; miRNA*; microRNA; mmu; pre-miRNA; precursor miRNA; pri-miRNA; primary miRNA; ssc