title: Pharmacokinetics study of hemin in rats by applying (58)Fe-extrinsically labeling techniques in combination with ICP-MS method.
abstract: Iron is a challenging element due to its high background in various matrixes including blood, tissues even in the air and it is urgent to develop a method for the accurate determination of iron in bio-samples. After optimization of mass spectrometric conditions using collision cell technology and compensating for interference using a mathematical correction equation, an inductively coupled plasma mass spectrometry (ICP-MS) method for the quantitative determination of (58)Fe originating from hemin extrinsically labeled avoiding endogenous interference was developed. After a single step of dilution, analysis of each sample was completed within 1.5min. The assay was linear in the concentration range from 0.005 to 1.0μg/ml. The precisions and accuracies determined within three consecutive days were in acceptable limits and there was no significant matrix effect. The optimized method was successfully applied to a pharmacokinetic study of (58)Fe originating from hemin extrinsically labeled and iron absorption measured in rats was 1.07%. Those indicated that extrinsically label techniques in combination with ICP-MS will become a new tool for the analysis of iron preparations and other endogenous substances.
authors.affiliation: Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China; Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Di
authors.initials: D
authors.lastname: Zhao
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Xu
authors.forename: Can
authors.initials: C
authors.lastname: Dong
authors.forename: Cuiyun
authors.initials: C
authors.lastname: Li
authors.forename: Shuangxia
authors.initials: S
authors.lastname: Ren
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.forename: Yue
authors.initials: Y
authors.lastname: Dai
authors.forename: Xijing
authors.initials: X
authors.lastname: Chen
mesh_terms: D000042:Absorption; D000818:Animals; D019540:Area Under Curve; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D004305:Dose-Response Relationship, Drug; D005260:Female; D006427:Hemin / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics*; D007501:Iron / Q000032:analysis / Q000737:chemistry; D007503:Iron Isotopes / Q000737:chemistry*; D007554:Isotopes / Q000737:chemistry; D016014:Linear Models; D008297:Male; D013058:Mass Spectrometry; D008962:Models, Theoretical; D004364:Pharmaceutical Preparations / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
chemical_list: D007503:Iron Isotopes; D007554:Isotopes; D004364:Pharmaceutical Preparations; D006427:Hemin; D007501:Iron
keywords: (58)Fe; Extrinsically labeling; Hemin; ICP-MS; Iron absorption; Pharmacokinetics