title: New procedure to synthesize silver nanoparticles and their interaction with local anesthetics.
abstract: Silver nanoparticles (AgNPs) were prepared in aqueous colloid dispersions by the reduction of Ag(+) with glucose in alkaline medium. Tetraethyl orthosilicate and L-asparagine were added as stabilizers of NPs. The AgNPs were characterized, and their interaction with three local anesthetics (procaine, dibucaine, or tetracaine) was investigated. Optical spectra show the characteristic absorption band of AgNPs, due to surface plasmon resonance. Modifications in the position and shape of this band reflect the self-assembly of metal NPs mediated by anesthetic molecules and the progress in time of the aggregation process. Zeta-potential measuring was applied in order to characterize the electrostatic stability of the NPs. The size and shape of the AgNPs, as well as the features of the assemblies formed by their association in the presence of anesthetics, were evidenced by transmission electron microscopy images. Atomic force microscopy images showed the characteristics of the films of AgNPs deposited on glass support. The effect of the anesthetics could be described in terms of electrostatic forces between the negatively charged AgNPs and the anesthetic molecules, existing also in their cationic form at the working pH. But also hydrophobic and hydrogen bonding interactions between the coated nanoparticles and anesthetics molecular species should be considered.
authors.affiliation: Chemical Engineering Department, Babes-Bolyai University, Cluj-Napoca, Romania.
authors.forename: Aurora
authors.initials: A
authors.lastname: Mocanu
authors.forename: Roxana Diana
authors.initials: RD
authors.lastname: Pasca
authors.forename: Gheorghe
authors.initials: G
authors.lastname: Tomoaia
authors.forename: Corina
authors.initials: C
authors.lastname: Garbo
authors.forename: Petre T
authors.initials: PT
authors.lastname: Frangopol
authors.forename: Ossi
authors.initials: O
authors.lastname: Horovitz
authors.forename: Maria
authors.initials: M
authors.lastname: Tomoaia-Cotisel
mesh_terms: D000327:Adsorption; D000779:Anesthetics, Local / Q000737:chemistry*; D001665:Binding Sites; D008422:Materials Testing / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D012834:Silver / Q000737:chemistry*
chemical_list: D000779:Anesthetics, Local; D012834:Silver
keywords: AFM; TEM; UV-vis spectra; self-assembled nanostructures; zeta potential