title: Winter wheat cells subjected to freezing temperature undergo death process with features of programmed cell death.
abstract: Programmed cell death is a process defined as genetically regulated self-destruction or cell suicide. It can be activated by different internal and external factors, but few studies have investigated whether this process occurs under cold and freezing temperatures. In this study, a freezing treatment (-8 °C for 6 h) induced cell death with features of programmed cell death in suspension cultures of winter wheat (Triticum aestivum L.). This process occurred for 10 days after cold exposure. The death of cells in culture was slow and prolonged, and was accompanied by protoplast shrinkage, DNA fragmentation, and an increase in the level of reactive oxygen species. Other changes observed after the freezing treatment included an increase in the respiration rate, changes in mitochondrial transmembrane potential (∆Ψ m ), and the release of cytochrome c from mitochondria into the cytosol. These findings indicated that mitochondria are involved in the cell death process that occurs after a freezing treatment in cells of winter wheat.
authors.affiliation: Siberian Institute of Plant Physiology and Biochemistry of Siberian Branch of Russian Academy of Sciences, Irkutsk-33, P.O. Box 317, Irkutsk, Russia, 664033.
authors.forename: Irina V
authors.initials: IV
authors.lastname: Lyubushkina
authors.forename: Olga I
authors.initials: OI
authors.lastname: Grabelnych
authors.forename: Tamara P
authors.initials: TP
authors.lastname: Pobezhimova
authors.forename: Aleksey V
authors.initials: AV
authors.lastname: Stepanov
authors.forename: Anna V
authors.initials: AV
authors.lastname: Fedyaeva
authors.forename: Irina V
authors.initials: IV
authors.lastname: Fedoseeva
authors.forename: Victor K
authors.initials: VK
authors.lastname: Voinikov
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D016923:Cell Death / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D045304:Cytochromes c / Q000378:metabolism; D053938:DNA Fragmentation; D004781:Environmental Exposure; D005615:Freezing; D008928:Mitochondria / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D014908:Triticum / Q000166:cytology / Q000378:metabolism / Q000502:physiology*
chemical_list: D017382:Reactive Oxygen Species; D045304:Cytochromes c