title: Inhibition of phenylpropanoid biosynthesis in Artemisia annua L.: a novel approach to reduce oxidative browning in plant tissue culture.
abstract: Oxidative browning is a common and often severe problem in plant tissue culture systems caused by the accumulation and oxidation of phenolic compounds. The current study was conducted to investigate a novel preventative approach to address this problem by inhibiting the activity of the phenylalanine ammonia lyase enzyme (PAL), thereby reducing the biosynthesis of phenolic compounds. This was accomplished by incorporating 2-aminoindane-2-phosphonic acid (AIP), a competitive PAL inhibitor, into culture media of Artemisia annua as a model system. Addition of AIP into culture media resulted in significant reductions in visual tissue browning, a reduction in total phenol content, as well as absorbance and autoflourescence of tissue extracts. Reduced tissue browning was accompanied with a significant increase in growth on cytokinin based medium. Microscopic observations demonstrated that phenolic compounds accumulated in discrete cells and that these cells were more prevalent in brown tissue. These cells were highly plasmolyzed and often ruptured during examination, demonstrating a mechanism in which phenolics are released into media in this system. These data indicate that inhibiting phenylpropanoid biosynthesis with AIP is an effective approach to reduce tissue browning in A. annua. Additional experiments with Ulmus americana and Acer saccharum indicate this approach is effective in many species and it could have a wide application in systems where oxidative browning restricts the development of biotechnologies.
authors.affiliation: Gosling Research Institute for Plant Preservation, Department of Plant Agriculture, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Andrew Maxwell Phineas
authors.initials: AM
authors.lastname: Jones
authors.forename: Praveen Kumar
authors.initials: PK
authors.lastname: Saxena
mesh_terms: D031002:Acer / Q000378:metabolism; D031172:Artemisia annua / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000187:drug effects; D003116:Color; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007189:Indans / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D063065:Organophosphonates / Q000494:pharmacology; D010084:Oxidation-Reduction / Q000187:drug effects; D019800:Phenol / Q000737:chemistry / Q000378:metabolism*; D010650:Phenylalanine Ammonia-Lyase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010858:Pigmentation / Q000187:drug effects; D010940:Plant Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D046509:Tissue Culture Techniques / Q000379:methods; D034403:Ulmus / Q000378:metabolism
chemical_list: C411146:2-aminoindan-2-phosphonic acid; D004791:Enzyme Inhibitors; D007189:Indans; D063065:Organophosphonates; D010940:Plant Proteins; D019800:Phenol; D010650:Phenylalanine Ammonia-Lyase