title: Making a protein extract from plant pathogenic fungi for gel- and LC-based proteomics.
abstract: Proteomic technologies have become a successful tool to provide relevant information on fungal biology. In the case of plant pathogenic fungi, this approach would allow a deeper knowledge of the interaction and the biological cycle of the pathogen, as well as the identification of pathogenicity and virulence factors. These two elements open up new possibilities for crop disease diagnosis and environment-friendly crop protection. Phytopathogenic fungi, due to its particular cellular characteristics, can be considered as a recalcitrant biological material, which makes it difficult to obtain quality protein samples for proteomic analysis. This chapter focuses on protein extraction for gel- and LC-based proteomics with specific protocols of our current research with Botrytis cinerea.
authors.affiliation: Agroforestry and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology, University of Córdoba, Agrifood Campus of International Excellence, Córdoba, Spain.
authors.forename: Raquel González
authors.initials: RG
authors.lastname: Fernández
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Redondo
authors.forename: Jesus V
authors.initials: JV
authors.lastname: Jorrin-Novo
mesh_terms: D020171:Botrytis / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005656:Fungal Proteins / Q000302:isolation & purification*; D010944:Plants / Q000382:microbiology*; D040901:Proteomics / Q000379:methods*
chemical_list: D005656:Fungal Proteins