title: Synthesis and biological evaluation of technetium-99m labeled galactose derivatives as potential asialoglycoprotein receptor probes in a hepatic fibrosis mouse model.
abstract: Two galactose derivatives, a monovalent (99m)Tc-MAMA-MGal galactoside and a divalent (99m)Tc-MAMA-DGal galactoside, were synthesized and radiolabeled in high radiochemical purity (>98%). Dynamic microSPECT imaging and biodistribution study of two traces in normal and liver fibrosis mice showed that the (99m)Tc-MAMA-DGal revealed higher specific binding to asialoglycoprotein receptors in liver and then rapidly excreted via both hepatobiliary system and renal clearance. The results suggest that (99m)Tc-MAMA-DGal may be used as SPECT probes for noninvasive evaluation of asialoglycoprotein receptor-related liver dysfunction.
authors.affiliation: Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, 155, Sec.2, Linong St., Taipei 11217, Taiwan.
authors.forename: Wen-Yi
authors.initials: WY
authors.lastname: Chang
authors.forename: Hao-Wen
authors.initials: HW
authors.lastname: Kao
authors.forename: Hsin-Ell
authors.initials: HE
authors.lastname: Wang
authors.forename: Jenn-Tzong
authors.initials: JT
authors.lastname: Chen
authors.forename: Wuu-Jyh
authors.initials: WJ
authors.lastname: Lin
authors.forename: Shyh-Jen
authors.initials: SJ
authors.lastname: Wang
authors.forename: Chuan-Lin
authors.initials: CL
authors.lastname: Chen
mesh_terms: D000818:Animals; D037263:Asialoglycoprotein Receptor / Q000032:analysis*; D004195:Disease Models, Animal; D005690:Galactose / Q000138:chemical synthesis* / Q000737:chemistry; D008103:Liver Cirrhosis / Q000000981:diagnostic imaging*; D051379:Mice; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000737:chemistry; D017556:Technetium Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods
chemical_list: D037263:Asialoglycoprotein Receptor; D019275:Radiopharmaceuticals; D017556:Technetium Compounds; D005690:Galactose
keywords: Asialoglycoprotein; Galactose; Liver function; SPECT; Technetium-99m