title: Two-stage implantation of the skin- and bone-integrated pylon seeded with autologous fibroblasts induced into osteoblast differentiation for direct skeletal attachment of limb prostheses.
abstract: Angio- and osteogenesis following the two-stage (TS) implantation of the skin- and bone-integrated pylon seeded with autologous fibroblasts was evaluated. Two consecutive animal substudies were undertaken: intramedullary subcutaneous implantation (15 rabbits) and a TS transcutaneous implantation (12 rabbits). We observed enhanced osseointegrative properties of the intramedullary porous component seeded with fibroblasts induced into osteoblast differentiation, as compared to the untreated porous titanium pylon. The three-phase scintigraphy and subsequent histological analysis showed that the level of osteogenesis was 1.5-fold higher than in the control group, and significantly so (p < 0.05). The biocompatibility was further proved by the absence of inflammatory response or encapsulation and sequestration on the histology assay. Treatment of the transcutaneous component with autologous fibroblasts was associated with nearly a 2-fold decrease in the period required for the ingrowth of dermal and subdermal soft tissues into the implant surface, as compared to the untreated porous titanium component. Direct dermal attachment to the transcutaneous implant prevented superficial and deep periprosthetic infections in rabbits in vivo.
authors.affiliation: Institute of Cytology of the Russian Academy of Sciences (RAS), St. Petersburg, 194064, Russian Federation; I.P. Pavlov State Medical University, St. Petersburg, 197022, Russian Federation.
authors.forename: Maxim A
authors.initials: MA
authors.lastname: Shevtsov
authors.forename: Oleg V
authors.initials: OV
authors.lastname: Galibin
authors.forename: Nataliya M
authors.initials: NM
authors.lastname: Yudintceva
authors.forename: Miralda I
authors.initials: MI
authors.lastname: Blinova
authors.forename: George P
authors.initials: GP
authors.lastname: Pinaev
authors.forename: Anna A
authors.initials: AA
authors.lastname: Ivanova
authors.forename: Olga N
authors.initials: ON
authors.lastname: Savchenko
authors.forename: Dmitriy N
authors.initials: DN
authors.lastname: Suslov
authors.forename: Igor L
authors.initials: IL
authors.lastname: Potokin
authors.forename: Emil
authors.initials: E
authors.lastname: Pitkin
authors.forename: Grigory
authors.initials: G
authors.lastname: Raykhtsaum
authors.forename: Mark R
authors.initials: MR
authors.lastname: Pitkin
mesh_terms: D000818:Animals; D001186:Artificial Limbs; D005347:Fibroblasts / Q000166:cytology / Q000637:transplantation*; D008297:Male; D016348:Osseointegration; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D011474:Prosthesis Design; D011817:Rabbits; D054457:Tissue Scaffolds / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry
chemical_list: D014025:Titanium
keywords: autologous fibroblasts; direct skeletal attachment; osseointegration; prosthetic rehabilitation; skin- and bone-integrated pylon