title: Multiplexing of combinatorial chemistry in antimycin biosynthesis: expansion of molecular diversity and utility.
abstract: Diversity-oriented biosynthesis of a library of antimycin-like compounds (380 altogether) was accomplished by using multiplex combinatorial biosynthesis. The core strategy depends on the use of combinatorial chemistry at different biosynthetic stages. This approach is applicable for the diversification of polyketides, nonribosomal peptides, and the hybrids that share a similar biosynthetic logic.
authors.affiliation: State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry (SIOC), Chinese Academy of Sciences (CAS), 345 Lingling Road, Shanghai 200032 (China).
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Lihan
authors.initials: L
authors.lastname: Zhang
authors.forename: Qingfei
authors.initials: Q
authors.lastname: Zheng
authors.forename: Ying
authors.initials: Y
authors.lastname: Han
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Daozhong
authors.initials: D
authors.lastname: Zhang
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Awakawa
authors.forename: Ikuro
authors.initials: I
authors.lastname: Abe
authors.forename: Wen
authors.initials: W
authors.lastname: Liu
mesh_terms: D000478:Alkylation; D000818:Animals; D000968:Antimycin A / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000494:pharmacology / Q000633:toxicity; D002176:Candida albicans / Q000187:drug effects; D002384:Catalysis; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020650:Combinatorial Chemistry Techniques; D003300:Copper / Q000737:chemistry; D014644:Genetic Variation; D007783:Lactones / Q000737:chemistry; D051379:Mice; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D061065:Polyketides / Q000378:metabolism; D054852:Small Molecule Libraries / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity
chemical_list: D007783:Lactones; D010455:Peptides; D061065:Polyketides; D054852:Small Molecule Libraries; C032456:antimycin; D000968:Antimycin A; D003300:Copper
keywords: antimycins; biosynthesis; combinatorial chemistry; dilactones; natural products