title: Gastric distension activates NUCB2/nesfatin-1-expressing neurons in the nucleus of the solitary tract.
abstract: Brainstem structures such as the nucleus of the solitary tract (NTS) and the dorsal motor nucleus of the vagus nerve (DMNX) are essential for the digestive function of the stomach. A large number of neurotransmitters including glutamate and gamma-aminobutyric acid (GABA) are involved in the central control of gastric functions. However, the neuropeptidergic systems implicated in this process remain undetermined. Nesfatin-1 was recently identified as a neuropeptide cleaved from the N-terminal part of NEFA/nucleobindin 2 precursor (NUCB2). Central administration of this neuropeptide inhibits food consumption and gastroduodenal motility in rodents. Interestingly, the NTS and the DMNX contain a dense population of NUCB2/nesfatin-1 cell bodies. These observations led us to investigate the possible involvement of NUCB2/nesfatin-1 neurons in the brainstem neuronal pathways that modulate gastric functions. We observed an activation of NTS NUCB2/nesfatinergic neurons after gastric distention in rats. In addition, we found that several NTS NUCB2/nesfatinergic neurons were GABAergic. Finally, when fluorogold was injected at the stomach level, many retrogradely labeled neurons were observed in the DMNX which were also positive for NUCB2/nesfatin-1. Taken together, these observations suggest for the first time that NUCB2/nesfatin-1 neurons of the NTS are sensitive to gastric distension and then may contribute to the satiety signal.
authors.affiliation: Physiology and Physiopathology of Motor and Autonomic Nervous Systems (PPSN, EA4667), University of Aix-Marseille, Marseille, France.
authors.forename: Marion S
authors.initials: MS
authors.lastname: Bonnet
authors.forename: Wassila
authors.initials: W
authors.lastname: Ouelaa
authors.forename: Vanessa
authors.initials: V
authors.lastname: Tillement
authors.forename: Jerôme
authors.initials: J
authors.lastname: Trouslard
authors.forename: André
authors.initials: A
authors.lastname: Jean
authors.forename: Bruno J
authors.initials: BJ
authors.lastname: Gonzalez
authors.forename: Guillaume
authors.initials: G
authors.lastname: Gourcerol
authors.forename: Michel
authors.initials: M
authors.lastname: Dallaporta
authors.forename: Jean-Denis
authors.initials: JD
authors.lastname: Troadec
authors.forename: Lourdes
authors.initials: L
authors.lastname: Mounien
mesh_terms: D000818:Animals; D001069:Appetite Regulation; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005968:Glutamate Decarboxylase / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D000080866:Nucleobindins; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D017552:Solitary Nucleus / Q000166:cytology / Q000502:physiology*; D013270:Stomach / Q000294:innervation / Q000502:physiology*; D014630:Vagus Nerve / Q000378:metabolism
chemical_list: D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; C000630651:Nucb1 protein, mouse; C000630652:Nucb1 protein, rat; D000080866:Nucleobindins; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1
keywords: ChAT; DMNX; DVC; FG; Food intake; GABA; GAD67; GD; GI; GIN; Gastric distension; LPS; NTS; NUCB2; NUCB2/nesfatin-1; Nucleobindin-2; choline acetyltransferase; dorsal motor nucleus of the vagus nerve; dorsal vagal complex; eGFP-expressing Inhibitory Neurons; fluorogold; gamma amino-butyric acid; gastric distension; gastro-intestinal; glutamic acid decarboxylase 67; lipopolysaccharide; nucleus of the solitary tract