title: Bi-enzyme sensor for phenolic compounds with fluorescent read-out.
abstract: In this paper, the use of tyrosinase (Ty) from Streptomyces antibioticus, labeled with a fluorescent tag, in combination with soluble quinoprotein (PQQ-containing) glucose dehydrogenase (s-GDH) to measure trace amounts of phenols is explored. Proof of concept is provided by a series of experiments, which show a clear quantitative dependence of the response on the phenol concentration. One of the advantages of the detection system is that apart from a standard fluorimeter no further instrumentation is required.
authors.affiliation: Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300RA Leiden (The Netherlands); Dipartimento di Chimica e Biologia,Università di Salerno via Giovanni Paolo II, 132, 84084 Fisciano (SA) (Italy). mstriane@unisa.it.
authors.forename: Maria
authors.initials: M
authors.lastname: Strianese
authors.forename: Gerhild
authors.initials: G
authors.lastname: Zauner
authors.forename: Leandro C
authors.initials: LC
authors.lastname: Tabares
authors.forename: Armand W J W
authors.initials: AW
authors.lastname: Tepper
authors.forename: Franco
authors.initials: F
authors.lastname: De Martino
authors.forename: Claudio
authors.initials: C
authors.lastname: Pellecchia
authors.forename: Thijs J
authors.initials: TJ
authors.lastname: Aartsma
authors.forename: Gerard W
authors.initials: GW
authors.lastname: Canters
mesh_terms: D015374:Biosensing Techniques; D004563:Electrochemistry; D004800:Enzymes, Immobilized; D005948:Glucose Dehydrogenases / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008667:Metalloproteins / Q000737:chemistry*; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D008667:Metalloproteins; D010636:Phenols; D005948:Glucose Dehydrogenases; C033955:glucose dehydrogenase (pyrroloquinoline-quinone); D014442:Monophenol Monooxygenase
keywords: FRET; biosensors; enzymes; metalloproteins