title: Construction of a plasmid for heterologous protein expression with a constitutive promoter in Trichoderma reesei.
abstract: Trichoderma reesei is widely used as a host for homologus and heterologus protein expression because of its well-known capability of protein secretion, especially cellulases secretion. In this study, a binary vector which could be used for protein expression was constructed with a constitutive promoter of rp2 gene. This vector contained the expression cassette Prp2-target gene-Trp2 and hygromycin B selection marker based on pCAMBIA1300 for T-DNA random insertion. The feasibility of the promoter was determined by eGFP (enhanced green fluorescence protein) expression in T. reesei. For heterologus protein expression, the expression of bgla from Aspergillus niger in the transformant was 3 folds higher than that of the wild type strain. The results demonstrate that this constitutive promoter could be applied for protein expression and thus this protein express system may contribute to the industrial protein production.
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
authors.forename: Ronglin
authors.initials: R
authors.lastname: He
authors.forename: Can
authors.initials: C
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.forename: Lixian
authors.initials: L
authors.lastname: Wang
authors.forename: Dongyuan
authors.initials: D
authors.lastname: Zhang
authors.forename: Shunlin
authors.initials: S
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D017930:Genes, Reporter; D005818:Genetic Engineering; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014170:Transformation, Genetic; D019076:Transgenes; D014242:Trichoderma / Q000235:genetics* / Q000378:metabolism; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D005656:Fungal Proteins; D012269:Ribosomal Proteins; C036483:T-DNA; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D001617:beta-Glucosidase
keywords: Heterologus expression; T. reesei; rp2 Promoter; β-Glucosidase