title: The rice nuclear gene WLP1 encoding a chloroplast ribosome L13 protein is needed for chloroplast development in rice grown under low temperature conditions.
abstract: Plastidial ribosome proteins (PRPs) form the major component of the plastidial ribosome. Here we describe a rice mutant named wlp1 (white leaf and panicles 1) selected from a population of tissue culture regenerants. The early seedling leaves of the mutant were albino, as was the immature panicle at heading, and the phenotype was more strongly expressed in plants exposed to low temperature conditions. Changes in the leaf pigmentation of the mutant were due to altered chlorophyll content and chloroplast development. Positional cloning of WLP1, followed by complementation and knock-down experiments, showed that it encodes a 50S ribosome L13 protein. The WLP1 protein localized to the chloroplast. WLP1 was mainly transcribed in green tissues and particularly abundantly in the early seedling leaves. In addition, the expression level of WLP1 was induced by the low temperature. The transcription pattern of a number of genes involved in plastidial transcription/translation and in photosynthesis was altered in the wlp1 mutants. These results reveal that WLP1 is required for normal chloroplast development, especially under low temperature conditions. This is the first report on the function of PRPs in rice.
authors.affiliation: State Key Laboratory of Rice Biology, Key Laboratory of Rice Biology and Breeding of Ministry of Agriculture, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Song
authors.forename: Xiangjin
authors.initials: X
authors.lastname: Wei
authors.forename: Gaoneng
authors.initials: G
authors.lastname: Shao
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Sheng
authors.forename: Daibo
authors.initials: D
authors.lastname: Chen
authors.forename: Congli
authors.initials: C
authors.lastname: Liu
authors.forename: Guiai
authors.initials: G
authors.lastname: Jiao
authors.forename: Lihong
authors.initials: L
authors.lastname: Xie
authors.forename: Shaoqing
authors.initials: S
authors.lastname: Tang
authors.forename: Peisong
authors.initials: P
authors.lastname: Hu
mesh_terms: D000595:Amino Acid Sequence; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000502:physiology*; D003001:Cloning, Molecular; D003080:Cold Temperature; D020869:Gene Expression Profiling; D017343:Genes, Plant; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics*; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D010788:Photosynthesis; D060888:Real-Time Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D009687:Nuclear Proteins; D012269:Ribosomal Proteins; D002734:Chlorophyll