title: Overexpression of microRna-200c in CD44+CD133+ CSCS inhibits the cellular migratory and invasion as well as tumorigenicity in mice.
abstract: Cancer stem cells (CSCs) are believed to be responsible for drug resistance, metastasis of tumors. To investigate the biological characteristics of CD44+CD133+CSCs with over- expressing microRNA-200c (miR-200c), and to provide evidences for miR-200c as a tumor suppressor to treat melanoma. CD44+CD133+CSCs were isolated from the mouse melanoma B16F10 cell line by using immune magnetic activated cell sorting. The lentivirus miR-200c was transduced into the cells, and the effect of miR-200c overexpression on the biological characteristics of B16F10 CD44+ CD133+CSCs was analyzed by a series assays. The stable overexpression of miR-200c in B16F10 CD44+CD133+CSCs obviously resulted in downregulation of zinc-finger E-box binding homeobox 1 expression, reduction of the cell proliferation, colony forming, cell migratory and invasion ability in vitro as well as tumorigenicity in vivo compared with those of the B16F10 cells and B16F10 non-CD44+ CD133+CSCs. These findings suggest that the miR-200c overexpression as a novel strategy to target therapy of melanoma CSCs.
authors.affiliation: Medical School, Southeast University Department of Pathogenic Biology and Immunology Nanjing China njdoujun&commat;yahoo.com.cn.
authors.forename: J
authors.initials: J
authors.lastname: Dou
authors.forename: X F
authors.initials: XF
authors.lastname: He
authors.forename: W H
authors.initials: WH
authors.lastname: Cao
authors.forename: F S
authors.initials: FS
authors.lastname: Zhao
authors.forename: X Y
authors.initials: XY
authors.lastname: Wang
authors.forename: Y R
authors.initials: YR
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Wang
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D063646:Carcinogenesis / Q000235:genetics* / Q000473:pathology; D002465:Cell Movement; D049109:Cell Proliferation; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D018960:Hyaluronan Receptors / Q000378:metabolism*; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism; D016086:Lentivirus / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology* / Q000821:virology; D010455:Peptides / Q000378:metabolism*; D014410:Tumor Stem Cell Assay; D000071799:Zinc Finger E-box-Binding Homeobox 1
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D018398:Homeodomain Proteins; D018960:Hyaluronan Receptors; D051741:Kruppel-Like Transcription Factors; D035683:MicroRNAs; C543719:Mirn200 microRNA, mouse; D010455:Peptides; C000606024:Prom1 protein, mouse; C519422:ZEB1 protein, mouse; D000071799:Zinc Finger E-box-Binding Homeobox 1