title: Growth and biochemical composition of Chlorella vulgaris in different growth media.
abstract: The need for clean and low-cost algae production demands for investigations on algal physiological response under different growth conditions. In this research, we investigated the growth, biomass production and biochemical composition of Chlorella vulgaris using semi-continuous cultures employing three growth media (LC Oligo, Chu 10 and WC media). The highest cell density was obtained in LC Oligo, while the lowest in Chu medium. Chlorophyll a, carbohydrate and protein concentrations and yield were highest in Chu and LC Oligo media. Lipid class analysis showed that hydrocarbons (HC), sterol esthers (SE), free fatty acids (FFA), aliphatic alcohols (ALC), acetone mobile polar lipids (AMPL) and phospholipids (PL) concentrations and yields were highest in the Chu medium. Triglyceride (TAG) and sterol (ST) concentrations were highest in the LC Oligo medium. The results suggested that for cost effective cultivation, LC Oligo medium is the best choice among those studied, as it saved the cost of buying vitamins and EDTA associated with the other growth media, while at the same time resulted in the best growth performance and biomass production.
authors.affiliation: Universidade Federal de São Carlos, Departamento de Botânica, São CarlosSP, Brasil.
authors.forename: Mathias A
authors.initials: MA
authors.lastname: Chia
authors.affiliation: Universidade Federal de São Carlos, Departamento de Botânica, São CarlosSP, Brasil.
authors.forename: Ana T
authors.initials: AT
authors.lastname: Lombardi
authors.affiliation: Universidade Federal de São Carlos, Departamento de Hidrobiologia, São CarlosSP, Brasil.
authors.forename: Maria da Graça G
authors.initials: Mda G
authors.lastname: Melão
mesh_terms: D020418:Algal Proteins / Q000032:analysis; D018533:Biomass; D002241:Carbohydrates / Q000032:analysis; D048408:Chlorella vulgaris / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development; D002734:Chlorophyll / Q000032:analysis; D000077194:Chlorophyll A; D003470:Culture Media / Q000494:pharmacology*; D008055:Lipids / Q000032:analysis
chemical_list: D020418:Algal Proteins; D002241:Carbohydrates; D003470:Culture Media; D008055:Lipids; D002734:Chlorophyll; D000077194:Chlorophyll A