title: Partially penetrant postnatal lethality of an epithelial specific MicroRNA in a mouse knockout.
abstract: MicroRNAs are small noncoding RNAs thought to have pivotal roles in numerous diseases and developmental processes. However, a growing body of literature indicates that in vivo elimination of these tiny RNAs usually has little to no observable consequence, suggesting functional redundancy with other microRNAs or cellular pathways. We provide an in-depth analysis of miR-205 expression and define miR-205 as an epithelial-specific microRNA, and for the first time show that ablation of this microRNA knockout exhibits partially penetrant lethality in a constitutive mouse knockout model. Given the role of this microRNA in cancer and development, this mouse model will be an incredible reagent to study the function and mechanisms of miR-205 in epithelial tissue development and disease.
authors.affiliation: Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California, United States of America ; UCSF Diabetes Center, University of California San Francisco, San Francisco, California, United States of America ; WM Keck Center for Noncoding RNAs, University of California San Francisco, San Francisco, California, United States of America.
authors.forename: D'Juan T
authors.initials: DT
authors.lastname: Farmer
authors.forename: Nikki
authors.initials: N
authors.lastname: Shariat
authors.forename: Chong Yon
authors.initials: CY
authors.lastname: Park
authors.forename: Huey Jiin
authors.initials: HJ
authors.lastname: Liu
authors.forename: Anastasia
authors.initials: A
authors.lastname: Mavropoulos
authors.forename: Michael T
authors.initials: MT
authors.lastname: McManus
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000196:embryology / Q000378:metabolism; D004848:Epithelium / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D005804:Genes, Lethal / Q000235:genetics*; D017403:In Situ Hybridization; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics*; D019683:Penetrance; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016019:Survival Analysis; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: C576666:MIRN205 microRNA, mouse; D035683:MicroRNAs; D001616:beta-Galactosidase