title: Photoelectrochemical biosensing platform for microRNA detection based on in situ producing electron donor from apoferritin-encapsulated ascorbic acid.
abstract: A novel signal "on" type of photoelectrochemical biosensor for microRNA-21 hybridization detection was fabricated, where Bi2S3 nanorods were used as photoactive material with a maximum adsorption at 450 nm visible light, hairpin-structure DNA as detecting probe, streptavidin as signal capturing unit and biotin functionalized ascorbic acid loaded apoferritin as signal amplification unit. Hybridization between the probe and the target microRNA-21 was confirmed by the increased photocurrent of the biosensor after electron donor of ascorbic acid was introduced into the detection buffer by digesting the apoferritin by trypsase, indicating that this method could be used fProd. Type: FTPor quantitative measurements, and the discrimination of the complementary from mismatched microRNA-21. Under the optimal detection conditions, the photoelectrochemical biosensor displayed a linear range of 1-5000 fM and a low detection limit of 0.35 fM for microRNA-21 determination. Moreover, the down-regulated expression of microRNA-21 in poultry cells and tissues infecting with avian leukosis viruses was confirmed by directly detecting microRNA-21 in extracted total RNA. This proposed strategy may open a new avenue for the applications of photoelectrochemical biosensor for oligonucleotides detection using visible light irradiation, which could largely reduce the destructive effect of UV light on biomolecules.
authors.affiliation: College of Chemistry and Material Science, Shandong Agricultural University, 271018 Taian, Shandong, PR China.
authors.forename: Huanshun
authors.initials: H
authors.lastname: Yin
authors.forename: Mo
authors.initials: M
authors.lastname: Wang
authors.forename: Yunlei
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhang
authors.forename: Bing
authors.initials: B
authors.lastname: Sun
authors.forename: Guihua
authors.initials: G
authors.lastname: Wang
authors.forename: Shiyun
authors.initials: S
authors.lastname: Ai
mesh_terms: D000327:Adsorption; D001052:Apoferritins / Q000302:isolation & purification*; D001205:Ascorbic Acid / Q000737:chemistry; D015374:Biosensing Techniques; D004583:Electrons; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D035683:MicroRNAs / Q000302:isolation & purification*; D043942:Nanotubes / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D014466:Ultraviolet Rays
chemical_list: C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D001052:Apoferritins; D001205:Ascorbic Acid
keywords: Apoferritin; Avian leukosis viruses; Bi(2)S(3) nanorods; MicroRNA detection; Photoelectrochemical biosensor