title: Association of osteopontin polymorphisms with nasopharyngeal carcinoma risk.
abstract: No previous study has reported the association of osteopontin polymorphisms with nasopharyngeal carcinoma (NPC) risk. We aimed to investigate the association in a Chinese population. Four variants of osteopontin, rs11730582, rs1126772, rs9138, and rs4754 polymorphisms, were assessed in a case-control study which consists of 108 NPC patients and 210 health controls, by using polymerase chain reaction--restriction fragment length polymorphism method. Serum osteopontin levels were measured by enzyme-linked immunosorbent assay. The serum osteopontin levels were significantly higher in NPC patients than those in controls (P<0.01). Carriers of CC and CT genotype of rs11730582 presented lower serum osteopontin levels than those of TT genotype carriers (P<0.05). Genotypes CT and CT+CC of rs11730582 were associated with the risk of NPC (CT:OR = 0.57, 95%CI = 0.34-0.94; CC+CT:OR = 0.54, 95%CI = 0.34-0.87). Haplotype analysis revealed that haplotype T-A-A-C of rs11730582, rs1126772, rs9138, and rs4754 was associated with NPC risk (OR = 0.49, 95%CI = 0.27-0.86). Stratification analysis showed that genotypes CT and CT+CC of rs11730582 were associated with tumor stage and lymph node metastasis (P<0.05). No associations were found between rs1126772, rs9138, and rs4754 polymorphisms and NPC risk (P>0.05). The variant rs11730582 of osteopontin is associated with NPC risk. It potentially serves as a genetic marker of NPC predisposition.
authors.affiliation: Center of Clinical Laboratory, Affiliated Hospital of Youjiang Medical College for Nationalities, Baise 533000, Guangxi, China.
authors.forename: Junli
authors.initials: J
authors.lastname: Wang
authors.affiliation: Center of Clinical Laboratory, Affiliated Hospital of Youjiang Medical College for Nationalities, Baise 533000, Guangxi, China.
authors.forename: Legen
authors.initials: L
authors.lastname: Nong
authors.affiliation: Center of Clinical Laboratory, Affiliated Hospital of Youjiang Medical College for Nationalities, Baise 533000, Guangxi, China.
authors.forename: Yesheng
authors.initials: Y
authors.lastname: Wei
authors.affiliation: First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Shanyu
authors.initials: S
authors.lastname: Qin
authors.affiliation: Minerva Foundation Institute for Medical Research, FI-00290 Helsinki, Finland. Electronic address: you.zhou@helsinki.fi.
authors.forename: You
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Affiliated Hospital of Youjiang Medical College for Nationalities, Baise 533000, Guangxi, China. Electronic address: baisetangyujin@163.com.
authors.forename: Yujin
authors.initials: Y
authors.lastname: Tang
mesh_terms: D000328:Adult; D002277:Carcinoma; D016022:Case-Control Studies; D004917:Erythromycin / Q000031:analogs & derivatives; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D053495:Osteopontin / Q000097:blood / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D053495:Osteopontin; C114264:Alemcinal; D004917:Erythromycin