title: Structure of liposome encapsulating proteins characterized by X-ray scattering and shell-modeling.
abstract: Lipid liposomes are promising drug delivery systems because they have superior curative effects owing to their high adaptability to a living body. Lipid liposomes encapsulating proteins were constructed and the structures examined using synchrotron radiation small- and wide-angle X-ray scattering (SR-SWAXS). The liposomes were prepared by a sequential combination of natural swelling, ultrasonic dispersion, freeze-throw, extrusion and spin-filtration. The liposomes were composed of acidic glycosphingolipid (ganglioside), cholesterol and phospholipids. By using shell-modeling methods, the asymmetric bilayer structure of the liposome and the encapsulation efficiency of proteins were determined. As well as other analytical techniques, SR-SWAXS and shell-modeling methods are shown to be a powerful tool for characterizing in situ structures of lipid liposomes as an important candidate of drug delivery systems.
authors.affiliation: Graduate School of Engineering, Gunma University, 4-2 Aramaki, Maebashi, Gunma 371-8510, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Hirai
authors.forename: Ryota
authors.initials: R
authors.lastname: Kimura
authors.forename: Kazuki
authors.initials: K
authors.lastname: Takeuchi
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Hagiwara
authors.forename: Rika
authors.initials: R
authors.lastname: Kawai-Hirai
authors.forename: Noboru
authors.initials: N
authors.lastname: Ohta
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Igarashi
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Shimuzu
mesh_terms: D008081:Liposomes; D008956:Models, Chemical; D011506:Proteins / Q000737:chemistry*; D012542:Scattering, Radiation; D014965:X-Rays
chemical_list: D008081:Liposomes; D011506:Proteins
keywords: DDS; liposome; solution X-ray scattering