title: Biological properties of bone marrow-derived early and late endothelial progenitor cells in different culture media.
abstract: Ex vivo expansion of endothelial progenitor cells (EPCs) may be a promising strategy to overcome the clinical problem of limited cell numbers. As the culture medium is the key for the cell characteristics, the effects of different culture media on EPCs were investigated in the present study. Rat bone marrow mononuclear cells were cultured in different media, including M-199 media with 20% fetal bovine serum (FBS) and bovine pituitary extract (M1); M-199 media with 10% FBS, 20 ng/ml vascular endothelial growth factor (VEGF) and 10 ng/ml basic fibroblast growth factor (bFGF; M2) or epidermal growth medium (EGM)-2MV media. The cell morphology and biological functions, such as proliferation, adhesion, migration, tube formation and nitric oxide (NO) production were subsequently assayed in vitro. Moreover, endothelial biomarkers and apoptosis were also analyzed. The results showed that endothelial‑like cells appeared in all of the culture systems. First‑passage cells, namely early EPCs, tended to form colonies in M2 and EGM-2MV media but showed a fusiform shape in M1 media. The 3rd or 4th generation EPCs, namely late EPCs, cultured in EGM-2MV media exhibited increased adhesion, migration, tube formation and NO production as compared with EPCs in M1 or M2 media. Furthermore, late EPCs cultured in EGM-2MV expressed higher levels of endothelial cell markers, such as von Willibrand factor (vWF)and CD31, but relatively greater levels of apoptosis were observed. In conclusion, cell culture conditions, for example the medium used, affects the biological properties of bone marrow-derived early and late EPCs.
authors.affiliation: Medicine Research Center, Weifang Medical University, Weifang, Shandong 261053, P.R. China.
authors.forename: Xiu M
authors.initials: XM
authors.lastname: Guan
authors.forename: Min
authors.initials: M
authors.lastname: Cheng
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Xiao D
authors.initials: XD
authors.lastname: Cui
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Yu L
authors.initials: YL
authors.lastname: Wang
authors.forename: Jin L
authors.initials: JL
authors.lastname: Sun
authors.forename: Xiao Y
authors.initials: XY
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001854:Bone Marrow Cells / Q000166:cytology*; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation; D048430:Cell Shape / Q000187:drug effects; D003114:Colony-Forming Units Assay; D003470:Culture Media / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000187:drug effects; D009569:Nitric Oxide / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*
chemical_list: D003470:Culture Media; D009569:Nitric Oxide
keywords: endothelial  progenitor  cells; cell  culture; mononuclear  cells; bone marrow