title: Synthesis of SiO₂ and CaO rich calcium silicate systems via sol-gel process: bioactivity, biocompatibility, and drug delivery tests.
abstract: Silica and calcium silicate amorphous materials, mixed with sodium ampicillin, a broad-spectrum antibiotic, have been synthesized by sol-gel method. The amorphous nature of the gels was ascertained by X-ray diffraction analysis. The bioactivity of the synthesized materials has been put into evidence by the appearance of a crystal of hydroxyapatite on the surface of the samples soaked in a fluid simulating the composition of the human blood plasma, as detected through FTIR measurements and SEM micrographs. The present work refers to a series of in-vitro biocompatibility tests, which has been performed on silicate and CaO rich calcium silicate gel-glasses, to study the cell behavior when seeded on 1 cm(2) material fragments, introduced into an in-vitro culture system. 3T3 cell lines have been used and the viability has been evaluated by WST-8 test. The composition of the adopted glasses can be expressed by the following general formula: x CaO• (1 - x) SiO2 with x = 0.00; 0.30; 0.40; 0.50; 0.60. Subsequently, release kinetics in a simulate body fluid (SBF) has been investigated. The amount of sodium ampicillin released has been detected by UV-Vis spectroscopy. The release kinetics has appeared to occur in more than one stage. All data have shown that those materials could be used as drug delivery bioactive systems.
authors.affiliation: Department of Industrial and Information Engineering, Second University of Naples, Via Roma 21, 81031, Aversa, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Catauro
authors.forename: F
authors.initials: F
authors.lastname: Papale
authors.forename: G
authors.initials: G
authors.lastname: Roviello
authors.forename: C
authors.initials: C
authors.lastname: Ferone
authors.forename: F
authors.initials: F
authors.lastname: Bollino
authors.forename: M
authors.initials: M
authors.lastname: Trifuoggi
authors.forename: C
authors.initials: C
authors.lastname: Aurilio
mesh_terms: D016475:3T3 Cells; D000667:Ampicillin / Q000008:administration & dosage; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D017610:Calcium Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D051379:Mice; D010087:Oxides / Q000737:chemistry*; D044367:Phase Transition; D017640:Silicates / Q000138:chemical synthesis / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004337:Drug Carriers; D010087:Oxides; D017640:Silicates; D012822:Silicon Dioxide; D000667:Ampicillin; C016538:lime; C031293:calcium silicate
keywords: bioactivity; biocompatibility; drug delivery; sol-gel synthesis