title: [Involvement of store-operated calcium channels and receptor-operated calcium channels in Ca(2+)-sensing receptor-evoked extracellular Ca(2+) influx and NO generation in human umbilical vein endothelial cells].
abstract: This paper aims to investigate the effect of store-operated calcium channels (SOC) and receptor-operated calcium channels (ROC) on Ca(2+)-sensing receptor (CaR)-induced extracellular Ca(2+) influx and nitric oxide (NO) generation in human umbilical vein endothelial cells (HUVEC). SOC blocker, non-selective cation channel blocker, ROC agonist and ROC blocker were used separately and combined. Intracellular Ca(2+) concentration ([Ca(2+)]i) was measured by Fura-2/AM loading. The activity of endothelial nitric oxide synthase (eNOS) and the production of NO were determined by the DAF-FM diacetate (DAF-FM DA). The results showed that increases of [Ca(2+)]i, eNOS activity and NO generation induced by CaR agonist Spermine were all reduced after single blocking the SOC or ROC, respectively (P < 0.05). ROC agonist can partially abolish the ROC blocker's effect (P < 0.05). The above mentioned effects evoked by CaR agonist Spermine were further reduced when blocking both SOC and ROC than single blocking SOC or ROC in HUVEC (P < 0.05). In conclusion, these results suggest that the SOC and ROC participate in the processes of CaR-evoked extracellular Ca(2+) influx and NO generation by a synergistic manner in HUVEC.
authors.affiliation: Department of Pathophysiology/Key Laboratory of Education Ministry of Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University, Shihezi 832002, China; Department of Lymphoma and Breast Medicine, Affiliated Tumor Hospital, Xinjiang Medical University, Urumqi 830011, China. fangf2002shz@126.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Xiao
authors.initials: X
authors.lastname: Liang
authors.forename: Hua
authors.initials: H
authors.lastname: Zhong
authors.forename: Chun-Jun
authors.initials: CJ
authors.lastname: Zhang
authors.forename: Fang
authors.initials: F
authors.lastname: He
mesh_terms: D002118:Calcium / Q000502:physiology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000502:physiology*; D020013:Calcium Signaling; D005452:Fluoresceins / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells / Q000502:physiology*; D006801:Humans; D009569:Nitric Oxide / Q000096:biosynthesis*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D044169:Receptors, Calcium-Sensing / Q000502:physiology*
chemical_list: C503301:4-amino-5-methylamino-2',7'-difluorofluorescein diacetate; D002121:Calcium Channel Blockers; D015220:Calcium Channels; D005452:Fluoresceins; D044169:Receptors, Calcium-Sensing; D009569:Nitric Oxide; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; D002118:Calcium