title: Expression changes and novel interaction partners of talin 1 in effector cells of autoimmune uveitis.
abstract: Autoimmune uveitis is characterized by crossing of blood-retinal barrier (BRB) by autoaggressive immune cells. Equine recurrent uveitis (ERU) is a valuable spontaneous model for autoimmune uveitis and analyses of differentially expressed proteins in ERU unraveled changed protein clusters in target tissues and immune system. Healthy eyes are devoid of leukocytes. In ERU, however, leukocytes enter the inner eye and subsequently destroy it. Molecular mechanisms enabling cell migration through BRB still remain elusive. Previously, we detected decreased talin 1 expression in blood-derived granulocytes of ERU cases, linking the innate immune system to ERU. Because changes in leukocyte protein expression pattern may play a role in pathological abnormalities leading to migration ability, we aimed at identifying interactors of talin 1 in leukocytes with immunoprecipitation, followed by LC-MS/MS for candidate identification. This enabled us to identify CD90 (Thy1) as novel interactor of talin 1 besides several other interactors. In blood-derived granulocytes from healthy individuals, CD90 was highly abundant and significantly reduced in ERU, especially in effector cells. Connection between talin 1 and CD90 and their expression differences in inflammation is an interesting novel finding allowing deeper insight into immune response of innate immune system and granulocyte migration ability in this organ-specific autoimmune disease.
authors.affiliation: Institute of Animal Physiology, Department of Veterinary Sciences and ‡Clinic of Small Animal Medicine, Center of Clinical Veterinary Medicine, LMU Munich , Veterinaerstr. 13, D-80539 Munich, Germany.
authors.forename: Roxane L
authors.initials: RL
authors.lastname: Degroote
authors.forename: Stefanie M
authors.initials: SM
authors.lastname: Hauck
authors.forename: Gudrun
authors.initials: G
authors.lastname: Treutlein
authors.forename: Barbara
authors.initials: B
authors.lastname: Amann
authors.forename: Kristina J H
authors.initials: KJ
authors.lastname: Fröhlich
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Kremmer
authors.forename: Juliane
authors.initials: J
authors.lastname: Merl
authors.forename: Manfred
authors.initials: M
authors.lastname: Stangassinger
authors.forename: Marius
authors.initials: M
authors.lastname: Ueffing
authors.forename: Cornelia A
authors.initials: CA
authors.lastname: Deeg
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000096:biosynthesis; D001327:Autoimmune Diseases; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D001813:Blood-Retinal Barrier; D016022:Case-Control Studies; D002465:Cell Movement; D002853:Chromatography, Liquid; D005786:Gene Expression Regulation; D006098:Granulocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D006734:Horse Diseases / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D006736:Horses; D047468:Immunoprecipitation; D013058:Mass Spectrometry; D058977:Molecular Sequence Annotation; D011485:Protein Binding; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D016608:Talin / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D018800:Thy-1 Antigens / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D014602:Uvea / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D014605:Uveitis / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*
chemical_list: D001323:Autoantibodies; D016608:Talin; D018800:Thy-1 Antigens