title: Electrodeposition of polydopamine thin films for DNA patterning and microarrays.
abstract: The controlled electrodeposition of functional polydopamine (PDA) thin films from aqueous dopamine solutions is demonstrated with a combination of electrochemistry, atomic force microscopy (AFM), and surface plasmon resonance (SPR) measurements. PDA micropatterns are then fabricated by electrodeposition on micrometer length scale gold electrodes and used for attaching amino-modified single-stranded DNA (ssDNA). After hybridization with fluorescently labeled ssDNA, the fluorescence microscopy characterization reveals that: (i) PDA can be toposelectively deposited at the microscale and (ii) electrochemically deposited PDA can be functionalized with amino-terminated ssDNA using the same chemistry as that for spontaneously deposited PDA. Finally, the application of electrodeposited PDA thin films to fabricate ssDNA microarrays is reported using SPR imaging (SPRI) measurements for the detection of DNA and DNA-modified gold nanoparticles.
authors.affiliation: Department of Chemistry, University of California-Irvine , Irvine, California 92697, United States.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Loget
authors.forename: Jennifer B
authors.initials: JB
authors.lastname: Wood
authors.forename: Kyunghee
authors.initials: K
authors.lastname: Cho
authors.forename: Aaron R
authors.initials: AR
authors.lastname: Halpern
authors.forename: Robert M
authors.initials: RM
authors.lastname: Corn
mesh_terms: D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D007211:Indoles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D020411:Oligonucleotide Array Sequence Analysis; D011108:Polymers / Q000737:chemistry*; D020349:Surface Plasmon Resonance
chemical_list: D005456:Fluorescent Dyes; D007211:Indoles; D011108:Polymers; C568283:polydopamine; D004247:DNA