title: [Effect of salt stress on DNA methylation in Isatis indigotica].
abstract: OBJECTIVE
  To investigate the effect of salt stress on DNA methylation in Isatis indigotica.
  
  
  METHODS
  Methylation sensitive amplification polymorphism (MSAP) approach was used in this study.
  
  
  RESULTS
  By using 15 pairs of selective primers combinations, a total of 632 MSAP bands were obtained in Isatis indigotica genome under salt stress. In all, 44 (6.9%) CCGG sites displayed cytosine methylation changes under salt stress. Among these sites, 31(4.9%) CCGG sites underwent hypermethylation changes and 13 (2.0%) CCGG sites underwent demethylation changes.
  
  
  CONCLUSIONS
  This result provides evidence for DNA methylation remodeling occurring immediately after salt stress affects Isatis indigotica.
authors.affiliation: College of Medicine, Yangtze University, Jingzhou 434025, China. yfwhu@163.com
authors.forename: Fei
authors.initials: F
authors.lastname: Yang
authors.forename: Hao
authors.initials: H
authors.lastname: Nie
authors.forename: Yan-Hao
authors.initials: YH
authors.lastname: Xu
mesh_terms: D019175:DNA Methylation; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D044127:Epigenesis, Genetic; D031219:Isatis / Q000187:drug effects* / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics; D011110:Polymorphism, Genetic; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D012965:Sodium Chloride