title: External validation of recombinant antigens for serodiagnosis of machine operator's lung.
abstract: BACKGROUND
  Machine operator's lung (MOL) is a hypersensitivity pneumonitis the diagnosis of which is difficult. Our laboratory previously developed an ELISA test using recombinant antigens from Mycobacterium immunogenum isolated in French plant. The objective was to validate the previous ELISA results with ten new suspected cases from Germany.
  
  
  METHODS
  Two serological analyses were performed: ELISA with the six recombinant antigens, and electrosyneresis with crude antigens of M. immunogenum and three other main species isolated from contaminated metalworking fluids.
  
  
  RESULTS
  The two recombinant antigens acyl-CoA dehydrogenase and dihydrolipoyl dehydrogenase, combined together, and electrosyneresis are useful in making the diagnosis regardless of the clinical and radiological data. Finally 9 out of the 10 suspected cases were declared as MOL.
  
  
  CONCLUSIONS
  Despite the geographical distance, the crude and recombinant antigens produced to investigate the clustered French cases also proved to be useful in diagnosing the suspected cases in Germany.
authors.affiliation: UMR/CNRS 6249 Chrono-Environnement, University of Franche-Comté, Besançon, France.
authors.forename: Coralie
authors.initials: C
authors.lastname: Barrera
authors.forename: Gabriel
authors.initials: G
authors.lastname: Reboux
authors.forename: Isabel
authors.initials: I
authors.lastname: Warfolomeow
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Rognon
authors.forename: Laurence
authors.initials: L
authors.lastname: Millon
authors.forename: Sandrine
authors.initials: S
authors.lastname: Roussel
mesh_terms: D042964:Acyl-CoA Dehydrogenase / Q000276:immunology; D000328:Adult; D000542:Alveolitis, Extrinsic Allergic / Q000175:diagnosis* / Q000382:microbiology; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D003378:Counterimmunoelectrophoresis; D008058:Dihydrolipoamide Dehydrogenase / Q000276:immunology; D020821:Dystonic Disorders / Q000175:diagnosis* / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D005602:France; D005858:Germany; D006801:Humans; D008297:Male; D008669:Metallurgy; D008875:Middle Aged; D009161:Mycobacterium / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D042964:Acyl-CoA Dehydrogenase; D008058:Dihydrolipoamide Dehydrogenase
keywords: Acyl-CoA dehydrogenase; Mycobacterium immunogenum; dihydrolipoyl dehydrogenase; hypersensitivity pneumonitis; metalworking fluid