title: Large scale study of HPV genotypes in cervical cancer and different cytological cervical specimens in Thailand.
abstract: Identification of high-risk HPV genotypes in patients is essential for vaccination and prevention programs while the geographic distribution of cervical cancer varies widely. HPV 16 is the major cause of cervical cancer followed by HPV 18, HPV 31, HPV 52, or HPV 58 depending on geographic area. In this study, the distribution of HPV genotypes in cervical specimens from women living in Thailand was analyzed by HPV testing with electrochemical DNA chip and PCR direct sequencing. The 716 specimens were grouped according to their cytological grades; 100 normal, 100 low-grade squamous intraepithelial lesions, 100 high grade squamous intraepithelial lesions, and 416 specimens of cervical cancer. The results showed that HPV 16, HPV 18, HPV 52, and HPV 58 are the most common HPV genotypes in Thailand, respectively. With respect to age, women below the age of 26 years were almost negative for high-risk HPV DNA exclusively. Conversely, high prevalence of high-risk HPV DNA and abnormal cytology were usually found in women between 26 and 45 years while cervical cancer was detected mainly in women above the age of 45 years. To increase protection efficiency, a vaccine including HPV 52 and HPV 58 should be offered to Asian women, and primary HPV screening should start at 26-30 years of age.
authors.affiliation: Center of Excellence in Clinical Virology, Faculty of Medicine, Department of Paediatrics, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Jira
authors.initials: J
authors.lastname: Chansaenroj
authors.forename: Pairoj
authors.initials: P
authors.lastname: Junyangdikul
authors.forename: Teeraporn
authors.initials: T
authors.lastname: Chinchai
authors.forename: Sukumarn
authors.initials: S
authors.lastname: Swangvaree
authors.forename: Anant
authors.initials: A
authors.lastname: Karalak
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Gemma
authors.forename: Yong
authors.initials: Y
authors.lastname: Poovorawan
mesh_terms: D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000145:classification* / Q000235:genetics*; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology; D017422:Sequence Analysis, DNA; D013785:Thailand / Q000453:epidemiology; D002578:Uterine Cervical Dysplasia / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology* / Q000821:virology
chemical_list: D004279:DNA, Viral
keywords: DNA chip; Thailand; multiple infections