title: Impact of seminal plasma superoxide dismutase and glutathione peroxidase on cryopreserved buffalo spermatozoa.
abstract: Fifty semen samples were collected from sixteen buffalo-bulls (4-10 years old) and evaluated before cryopreservation. The activity of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) as well as the levels of glutathione (GSH) and malondialdehyde (MDA) were assayed in the seminal plasma before freezing. Aspartate aminotransferase (AST) activity and cholesterol content were assayed in seminal plasma before freezing and after thawing. Results revealed the presence of SOD and GPx activities (0.07 ± 0.01 U/ml and 14.59 ± 0.50 nmol/min/ml, respectively) in buffaloes' seminal plasma. SOD activity was positively correlated with both of GSH level and GST activity in seminal plasma, and showed an inverse relationship with both cholesterol efflux and post-thaw abnormal tails of buffalo spermatozoa. A positive correlation was found between GPx activity in seminal plasma and abnormal tails and an inverse relationship with both post-thaw viability indices and increased motility in response to PTx. GST activity showed a positive correlation with the increased motility after addition of PTx and negative correlations with both of cholesterol level and AST activity. MDA levels were negatively correlated with motility after addition of PTx and positive correlations with both post-thaw abnormal acrosomes and tails. Buffalo seminal plasma contains high activities of SOD, GPx and GST enzymes and GSH levels that have an influence on the functional competence of cryopreserved spermatozoa.
authors.affiliation: Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12515, Egypt; Department of Clinical Studies, College of Veterinary Medicine and Animal Resources, King Faisal University PO Box 400, Al-Hufof 31982, Saudi Arabia. Electronic address: mmwaheed@kfu.edu.sa.
authors.forename: M M
authors.initials: MM
authors.lastname: Waheed
authors.forename: Eman M
authors.initials: EM
authors.lastname: Gouda
authors.forename: T A A
authors.initials: TA
authors.lastname: Khalifa
mesh_terms: D000177:Acrosome / Q000201:enzymology / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002020:Buffaloes / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D015925:Cryopreservation / Q000662:veterinary; D005979:Glutathione Peroxidase / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D012661:Semen / Q000201:enzymology* / Q000378:metabolism; D055101:Semen Analysis / Q000662:veterinary; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary; D013094:Spermatozoa / Q000201:enzymology / Q000378:metabolism; D013482:Superoxide Dismutase / Q000502:physiology*
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Buffalo; Cryopreservation; Glutathione peroxidase.; Semen; Superoxide dismutase