title: In vivo evaluation of the antimutagenic and antigenotoxic effects of β-glucan extracted from Saccharomyces cerevisiae in acute treatment with multiple doses.
abstract: Ample evidence suggests that cancer is triggered by mutagenic damage and diets or supplements capable of reducing such incidences can be related to the prevention of neoplasy development or to an improvement in life quality of patients who undergo chemotherapy. This research aimed to evaluate the antimutagenic and antigenotoxic activity of β-glucan. We set up 8 experimental groups: control (Group 1), cyclophosphamide (Group 2), Groups 3-5 to assess the effect of β-glucan administration, and Groups 6-8 to evaluate the association between cyclophosphamide and β-glucan. The intraperitonial concentrations of β-glucan used were 100, 150 and 200 mg/kg. Micronucleus and comet assays showed that within the first week of treatment β-glucan presented a damage reduction rate between 100-62.04% and 94.34-59.52% for mutagenic and genotoxic damages, respectively. This activity decreased as the treatment was extended. During the sixth week of treatment antimutagenicity rates were reduced to 59.51-39.83% and antigenotoxicity was not effective. This leads to the conclusion that the efficacy of β-glucan in preventing DNA damage is limited when treatment is extended, and that its use as a chemotherapeutic adjuvant need to be better clarified.
authors.affiliation: Centro de Estudos em Célula Tronco, Terapia Celular e Genética Toxicológica, Núcleo de Hospital Universitário, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil . ; Programa de Pós-graduação em Saúde em Desenvolvimento na Região Centro-Oeste, Faculdade de Medicina "Dr. Hélio Mandetta", Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil . ; Programa de Mestrado em Farmácia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil .
authors.forename: Rodrigo Juliano
authors.initials: RJ
authors.lastname: Oliveira
authors.forename: Maria José Sparça
authors.initials: MJ
authors.lastname: Salles
authors.forename: Ariane Fernanda
authors.initials: AF
authors.lastname: da Silva
authors.forename: Tatiane Yumi Nakamura
authors.initials: TY
authors.lastname: Kanno
authors.forename: Ana Carolina Dos Santos
authors.initials: AC
authors.lastname: Lourenço
authors.forename: Véssia da Silva
authors.initials: Vda S
authors.lastname: Leite
authors.forename: Hevenilton José
authors.initials: HJ
authors.lastname: Matiazi
authors.forename: João Renato
authors.initials: JR
authors.lastname: Pesarini
authors.forename: Lúcia Regina
authors.initials: LR
authors.lastname: Ribeiro
authors.forename: Mário Sérgio
authors.initials: MS
authors.lastname: Mantovani
keywords: antigenotoxicity; antimutagenicity; cyclophosphamide; mice; β-glucan