title: Ultrahigh-throughput sorting of microfluidic drops with flow cytometry.
abstract: The detection and sorting of aqueous drops is central to microfluidic workflows for high-throughput biology applications, including directed evolution, digital PCR, and antibody screening. However, high-throughput detection and sorting of drops require optical systems and microfluidic components that are complex, difficult to build, and often yield inadequate sensitivity and throughput. Here, we demonstrate a general method to harness flow cytometry, with its unmatched speed and sensitivity, for droplet-based microfluidic sorting.
authors.affiliation: UC Berkeley-UCSF Graduate Program in Bioengineering, University of California, San Francisco, San Francisco, California, USA.
authors.forename: Shaun W
authors.initials: SW
authors.lastname: Lim
authors.forename: Adam R
authors.initials: AR
authors.lastname: Abate
mesh_terms: D000818:Animals; D002417:Cattle; D017931:DNA Primers / Q000378:metabolism; D004655:Emulsions / Q000737:chemistry; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D005452:Fluoresceins / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009821:Oils / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D019914:Taq Polymerase / Q000378:metabolism; D014867:Water / Q000737:chemistry
chemical_list: D017931:DNA Primers; D004655:Emulsions; D005452:Fluoresceins; D009821:Oils; D014867:Water; D012710:Serum Albumin, Bovine; C024098:6-carboxyfluorescein; D019914:Taq Polymerase