title: Proteomic analysis of a rare urinary stone composed of calcium carbonate and calcium oxalate dihydrate: a case report.
abstract: The objective of the present study was to investigate the matrix protein of a rare urinary stone that contained calcium carbonate. A urinary stone was extracted from a 34-year-old male patient with metabolic alkalosis. After X-ray diffractometry and infrared analysis of the stone, proteomic analysis was carried out. The resulting mass spectra were evaluated with protein search software, and matrix proteins were identified. X-ray diffraction and infrared analysis confirmed that the stone contained calcium carbonate and calcium oxalate dihydrate. Of the identified 53 proteins, 24 have not been previously reported from calcium oxalate- or calcium phosphate-containing stones. The protease inhibitors and several proteins related to cell adhesion or the cytoskeleton were identified for the first time. We analyzed in detail a rare urinary stone composed of calcium carbonate and calcium oxalate dihydrate. Considering the formation of a calcium carbonate stone, the new identified proteins should play an important role on the urolithiasis process in alkaline condition.
authors.affiliation: Laboratory of Biomedical and Analytical Sciences, Faculty of Pharma Sciences, Teikyo University, Tokyo, Japan.
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Kaneko
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Matsuta
authors.forename: Manabu
authors.initials: M
authors.lastname: Moriyama
authors.forename: Makoto
authors.initials: M
authors.lastname: Yasuda
authors.forename: Noriharu
authors.initials: N
authors.lastname: Chishima
authors.forename: Noriko
authors.initials: N
authors.lastname: Yamaoka
authors.forename: Tomoko
authors.initials: T
authors.lastname: Fukuuchi
authors.forename: Katsuhito
authors.initials: K
authors.lastname: Miyazawa
authors.forename: Koji
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D000328:Adult; D002119:Calcium Carbonate / Q000032:analysis*; D002129:Calcium Oxalate / Q000032:analysis*; D006801:Humans; D008297:Male; D040901:Proteomics; D014514:Ureteral Calculi / Q000737:chemistry*
chemical_list: D002129:Calcium Oxalate; D002119:Calcium Carbonate
keywords: X-ray diffraction; a rare urinary stone; calcium carbonate; matrix protein; proteomic analysis