title: Acquisition of thermotolerant yeast Saccharomyces cerevisiae by breeding via stepwise adaptation.
abstract: A thermotolerant Saccharomyces cerevisiae yeast strain, YK60-1, was bred from a parental strain, MT8-1, via stepwise adaptation. YK60-1 grew at 40°C, a temperature at which MT8-1 could not grow at all. YK60-1 exhibited faster growth than MT8-1 at 30°C. To investigate the mechanisms how MT8-1 acquired thermotolerance, DNA microarray analysis was performed. The analysis revealed the induction of stress-responsive genes such as those encoding heat shock proteins and trehalose biosynthetic enzymes in YK60-1. Furthermore, nontargeting metabolome analysis showed that YK60-1 accumulated more trehalose, a metabolite that contributes to stress tolerance in yeast, than MT8-1. In conclusion, S. cerevisiae MT8-1 acquired thermotolerance by induction of specific stress-responsive genes and enhanced intracellular trehalose levels.
authors.affiliation: Div. of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Satomura
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Katsuyama
authors.forename: Natsuko
authors.initials: N
authors.lastname: Miura
authors.forename: Kouichi
authors.initials: K
authors.lastname: Kuroda
authors.forename: Ayako
authors.initials: A
authors.lastname: Tomio
authors.forename: Takeshi
authors.initials: T
authors.lastname: Bamba
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Fukusaki
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Ueda
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D008401:Gas Chromatography-Mass Spectrometry; D015966:Gene Expression Regulation, Fungal; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006358:Hot Temperature; D055432:Metabolomics; D020411:Oligonucleotide Array Sequence Analysis; D012331:RNA, Fungal / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D014199:Trehalose / Q000378:metabolism
chemical_list: D006360:Heat-Shock Proteins; D012331:RNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D014199:Trehalose
keywords: Saccharomyces cerevisiae; breeding; thermotolerant yeast; trehalose