title: Prediction and validation of the unexplored RNA-binding protein atlas of the human proteome.
abstract: Detecting protein-RNA interactions is challenging both experimentally and computationally because RNAs are large in number, diverse in cellular location and function, and flexible in structure. As a result, many RNA-binding proteins (RBPs) remain to be identified. Here, a template-based, function-prediction technique SPOT-Seq for RBPs is applied to human proteome and its result is validated by a recent proteomic experimental discovery of 860 mRNA-binding proteins (mRBPs). The coverage (or sensitivity) is 42.6% for 1217 known RBPs annotated in the Gene Ontology and 43.6% for 860 newly discovered human mRBPs. Consistent sensitivity indicates the robust performance of SPOT-Seq for predicting RBPs. More importantly, SPOT-Seq detects 2418 novel RBPs in human proteome, 291 of which were validated by the newly discovered mRBP set. Among 291 validated novel RBPs, 61 are not homologous to any known RBPs. Successful validation of predicted novel RBPs permits us to further analysis of their phenotypic roles in disease pathways. The dataset of 2418 predicted novel RBPs along with confidence levels and complex structures is available at http://sparks-lab.org (in publications) for experimental confirmations and hypothesis generation.
authors.affiliation: School of Informatics and Computing, Indiana University Purdue University, Indianapolis, Indiana, 46202; Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, Indiana, 46202.
authors.forename: Huiying
authors.initials: H
authors.lastname: Zhao
authors.forename: Yuedong
authors.initials: Y
authors.lastname: Yang
authors.forename: Sarath Chandra
authors.initials: SC
authors.lastname: Janga
authors.forename: C Cheng
authors.initials: CC
authors.lastname: Kao
authors.forename: Yaoqi
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D001665:Binding Sites / Q000235:genetics; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000737:chemistry; D030562:Databases, Protein; D006367:HeLa Cells; D006801:Humans; D011485:Protein Binding; D020543:Proteome; D040901:Proteomics; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D020543:Proteome; D016601:RNA-Binding Proteins
keywords: DNA binding proteins; KEGG; RNA binding proteins; human proteome