title: Membrane lipid profile alterations are associated with the metabolic adaptation of the Caco-2 cells to aglycemic nutritional condition.
abstract: Cancer cells can adapt their metabolic activity under nutritional hostile conditions in order to ensure both bioenergetics and biosynthetic requirements to survive. In this study, the effect of glucose deprivation on Caco-2 cells bioenergetics activity and putative relationship with membrane lipid changes were investigated. Glucose deprivation induces a metabolic remodeling characterized at mitochondrial level by an increase of oxygen consumption, arising from an improvement of complex II and complex IV activities and an inhibition of complex I activity. This effect is accompanied by changes in cellular membrane phospholipid profile. Caco-2 cells grown under glucose deprivation show higher phosphatidylethanolamine content and decreased phosphatidic acid content. Considering fatty acid profile of all cell phospholipids, glucose deprivation induces a decrease of monounsaturated fatty acid (MUFA) and n-3 polyunsaturated fatty acids (PUFA) simultaneously with an increase of n-6 PUFA, with consequent drop of n-3/n-6 ratio. Additionally, glucose deprivation affects significantly the fatty acid profile of all individual phospholipid classes, reflected by an increase of peroxidability index in zwitterionic phospholipids and a decrease in all anionic phospholipids, including mitochondrial cardiolipin. These data indicate that Caco-2 cells metabolic remodeling induced by glucose deprivation actively involves membrane lipid changes associated with a specific bioenergetics profile which ensure cell survival.
authors.forename: Vera F
authors.initials: VF
authors.lastname: Monteiro-Cardoso
authors.forename: Amélia M
authors.initials: AM
authors.lastname: Silva
authors.forename: Maria M
authors.initials: MM
authors.lastname: Oliveira
authors.forename: Francisco
authors.initials: F
authors.lastname: Peixoto
authors.forename: Romeu A
authors.initials: RA
authors.lastname: Videira
mesh_terms: D018938:Caco-2 Cells; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000502:physiology; D004734:Energy Metabolism; D005947:Glucose / Q000172:deficiency* / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation; D008563:Membrane Lipids / Q000378:metabolism*
chemical_list: D008563:Membrane Lipids; D005947:Glucose