title: Rapid synthesis of propyl caffeate in ionic liquid using a packed bed enzyme microreactor under continuous-flow conditions.
abstract: Propyl caffeate has the highest antioxidant activity among caffeic acid alkyl esters, but its industrial production via enzymatic transesterification in batch reactors is hindered by a long reaction time (24h). To develop a rapid process for the production of propyl caffeate in high yield, a continuous-flow microreactor composed of a two-piece PDMS in a sandwich-like microchannel structure was designed for the transesterification of methyl caffeate and 1-propanol catalyzed by Novozym 435 in [B mim][CF3SO3]. The maximum yield (99.5%) in the microreactor was achieved in a short period of time (2.5h) with a flow rate of 2 μL/min, which kinetic constant Km was 16 times lower than that of a batch reactor. The results indicated that the use of a continuous-flow packed bed enzyme microreactor is an efficient method of producing propyl caffeate with an overall yield of 84.0%.
authors.affiliation: School of the Environment, Jiangsu University, Zhenjiang 212013, PR China; School of Biology and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China. Electronic address: wangjun@just.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Shuang-Shuang
authors.initials: SS
authors.lastname: Gu
authors.forename: Hong-Sheng
authors.initials: HS
authors.lastname: Cui
authors.forename: Liu-Qing
authors.initials: LQ
authors.lastname: Yang
authors.forename: Xiang-Yang
authors.initials: XY
authors.lastname: Wu
mesh_terms: D061249:Batch Cell Culture Techniques; D055162:Biocatalysis / Q000187:drug effects; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D002109:Caffeic Acids / Q000378:metabolism*; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D052578:Ionic Liquids / Q000494:pharmacology*; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D008904:Miniaturization; D059027:Recycling; D012212:Rheology; D013696:Temperature
chemical_list: D002109:Caffeic Acids; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D052578:Ionic Liquids; C000588778:propyl caffeate; C093667:Novozyme 435; D008049:Lipase; C040048:caffeic acid
keywords: Biocatalysis; Continuous-flow condition; Packed bed enzyme microreactor; Propyl caffeate; Transesterification