title: Lipopolysaccharide-induced apoptosis in a murine intestinal endocrine cell line by modulation of Bcl-2, Bax and caspase-3.
abstract: Numerous studies have focused on how to modulate the secretion of glucagon-like peptide 1 (GLP-1) due to its marked anti-diabetic function. However, few studies have investigated the apoptosis of enteroendocrine L cells, which secrete GLP-1. The aim of this study was to determine whether lipopolysaccharide (LPS), a gut bacterial product, is capable of inducing apoptosis in the intestinal endocrine cell line STC-1. We found that LPS is capable of reducing the viability of STC-1 cells in a concentration-dependent manner. annexin V/propidium iodide (PI) double staining detected by fluorescence microscopy and flow cytometry revealed a strong apoptosis-inducing ability for LPS in STC-1 cells. Furthermore, western blotting revealed that exposure to LPS significantly decreased the expression of Bcl-2 and increased the expression of Bax and caspase-3. In conclusion, LPS induced the apoptosis of STC-1 cells in a dose-dependent manner, which may be responsible for the reduced secretion of GLP-1 in diabetes.
authors.affiliation: Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Lei
authors.forename: Jiao
authors.initials: J
authors.lastname: Wang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Dehong
authors.initials: D
authors.lastname: Cai
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism*; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D055098:Endocrine Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D005434:Flow Cytometry; D007422:Intestines / Q000166:cytology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D051028:bcl-2-Associated X Protein; D053148:Caspase 3
keywords: lipopolysaccharide; apoptosis; glucagon-like peptide 1; enteroendocrine L cells