title: Dynamic expression of miR-126* and its effects on proliferation and contraction of hepatic stellate cells.
abstract: In our previous study, miR-126 was identified as one of the leading miRNAs that is downregulated during activation of hepatic stellate cells (HSCs). However, the roles and related mechanisms of miR-126 in HSCs are not understood. In this study, we compared expression of miR-126 during HSC activation both in vitro and in vivo. We also applied RNA interference to analyze the role and mechanism of miR-126(*) in the activation of HSCs. Restoring HSCs with Lv-miR-126(*) resulted in decreased proliferation, accumulation of extracellular matrix components, and cell contraction, while also negatively regulating the vascular endothelial growth factor (VEGF) signal transduction pathways by partially targeted VEGF-A. Thus, we postulate that miR-126 may be a biological marker for the activation of HSCs, and useful for reducing intrahepatic vascular resistance and improving the sinusoidal microcirculation in chronic liver diseases.
authors.affiliation: Digestive Disease Laboratory and Department of Gastroenterology, Ren Ji Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200001, China.
authors.forename: Can-Jie
authors.initials: CJ
authors.lastname: Guo
authors.forename: Qin
authors.initials: Q
authors.lastname: Pan
authors.forename: Hua
authors.initials: H
authors.lastname: Xiong
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Qiao
authors.forename: Zhao-Lian
authors.initials: ZL
authors.lastname: Bian
authors.forename: Wei
authors.initials: W
authors.lastname: Zhong
authors.forename: Li
authors.initials: L
authors.lastname: Sheng
authors.forename: Hai
authors.initials: H
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Shen
authors.forename: Jing
authors.initials: J
authors.lastname: Hua
authors.forename: Xiong
authors.initials: X
authors.lastname: Ma
authors.forename: Jing-Yuan
authors.initials: JY
authors.lastname: Fang
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D005109:Extracellular Matrix / Q000378:metabolism; D055166:Hepatic Stellate Cells / Q000378:metabolism* / Q000502:physiology; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D014158:Transcription, Genetic; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: C571458:MIRN126 microRNA, rat; D035683:MicroRNAs; D042461:Vascular Endothelial Growth Factor A
keywords: 3′UTR; 3′untranslated region; Akt; COL IV; Chronic liver disease; ECM; EGFL7; GO; HA; HE; HSC; Hepatic stellate cell; KEGG; LN; PCIII; PI3K; Portal hypertension; VEGF; VEGFA; VG; Van Gieson; epidermal growth factor-1ike domain 7; extracellular matrix; gene ontology; hematoxylin/eosin; hepatic stellate cell; hyalaronic acid; kyoto encyclopedia of genes and genomes; laminin; miR-126(∗); miRNA; microRNA; phosphatidylinositol-3-kinase; procollagen typeIII; protein kinase B; type IV collagen; vascular endothelial growth factor