title: Amaranth 7S globulin Langmuir films and its interaction with l-α-dipalmitoilphosphatidilcholine at the air-fluid interface.
abstract: Amaranth seeds are one of the more promising food ingredients, due to their high protein content, among which the most important are storage proteins known as globulins. However, little is known about the physicochemical of the globulin proteins. In this work, we study the physicochemical behavior of films made of amaranth 7S globulin and its interaction with a model membrane made of L-α-dipalmitoylphosphatidylcholine (L-α-DPPC) at the air-liquid interface. The study was done by means of Langmuir balance, Brewster angle microscopy (BAM), fluorescence microscopy, and atomic force microscopy (AFM). We found that isotherms of pure 7S globulin directly deposited on either water or buffer subphases behave similarly and globulin forms a condensed film made of globular and denature structures, which was confirmed by BAM observations. Good mixtures of the protein with L-α-DPPC are formed at low surface pressure. However, they phase separate from moderate to high surface pressure as observed by BAM. Isotherms detect the presence of the protein in the mixture with L-α-DPPC, but we were unable to detect it through BAM or AFM. We show that fluorescence microscopy is a very good technique to detect the presence of the protein when it is well-mixed within the LE phase of the lipid. AFM images clearly show the formation of protein mono- and multilayers, and in phase mode, we detected domains that are formed by protein and LE lipid phase, which were corroborated by fluorescence microscopy. We have shown that globulin 7S mix well with lipid phases, which could be important in food applications as stabilizers or emulsifiers, but we also show that they can phase separate with a moderate to high surface pressure.
authors.affiliation: Colloids and Interfaces Laboratory, Institute of Physics, Autonomous University of San Luis Potosi , Alvaro Obregon 64, 78000 San Luis Potosi, S.L.P., Mexico.
authors.forename: Alcione
authors.initials: A
authors.lastname: Garcia-Gonzalez
authors.forename: A L
authors.initials: AL
authors.lastname: Flores-Vazquez
authors.forename: A P
authors.initials: AP
authors.lastname: Barba de la Rosa
authors.forename: E A
authors.initials: EA
authors.lastname: Vazquez-Martinez
authors.forename: J
authors.initials: J
authors.lastname: Ruiz-Garcia
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry / Q000378:metabolism*; D000388:Air; D027721:Amaranthus / Q000737:chemistry / Q000378:metabolism*; D005916:Globulins / Q000737:chemistry / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D011312:Pressure; D012639:Seeds / Q000737:chemistry / Q000378:metabolism; D014867:Water / Q000737:chemistry
chemical_list: D005916:Globulins; D010940:Plant Proteins; D014867:Water; D015060:1,2-Dipalmitoylphosphatidylcholine