title: Lactoferrin inhibits dexamethasone-induced chondrocyte impairment from osteoarthritic cartilage through up-regulation of extracellular signal-regulated kinase 1/2 and suppression of FASL, FAS, and Caspase 3.
abstract: Dexamethasone (Dex) is commonly used for osteoarthritis (OA) with excellent anti-inflammatory and analgesic effect. However, Dex also has many side effects following repeated use over prolonged periods mainly through increasing apoptosis and inhibiting proliferation. Lactoferrin (LF) exerts significantly anabolic effect on many cells and little is known about its effect on OA chondrocytes. Therefore, the aim of this study is to investigate whether LF can inhibit Dex-induced OA chondrocytes apoptosis and explore its possible molecular mechanism involved in. MTT assay was used to determine the optimal concentration of Dex and recombinant human LF (rhLF) on chondrocytes at different time and dose points. Chondrocytes were then stimulated with Dex in the absence or presence of optimal concentration of rhLF. Cell proliferation and viability were evaluated using MTT and LIVE/DEAD assay, respectively. Cell apoptosis was evaluated by multi-parameter apoptosis assay kit using both confocal microscopy and flow cytometry, respectively. The expression of extracellular signal-regulated kinase (ERK), FAS, FASL, and Caspase-3 (CASP3) at the mRNA and protein levels were examined by real-time polymerase chain reaction (PCR) and immunocytochemistry, respectively. The optimal concentration of Dex (25 μg/ml) and rhLF (200 μg/ml) were chosen for the following experiments. rhLF significantly reversed the detrimental effect of Dex on chondrocytes proliferation, viability, and apoptosis. In addition, rhLF significantly prevented Dex-induced down-regulation of ERK and up-regulation of FAS, FASL, and CASP3. These findings demonstrated that rhLF acts as an anabolic effect on chondrocytes through significantly reversing Dex-induced chondrocytes apoptosis. This study may contribute to further investigating the clinical application of LF on OA.
authors.affiliation: Department of Orthopaedics, Yangpu District Central Hospital Affiliated to Tongji University School of Medicine, 450 Tengyue Road, Shanghai, China.
authors.forename: Yihui
authors.initials: Y
authors.lastname: Tu
authors.forename: Huaming
authors.initials: H
authors.lastname: Xue
authors.forename: Wendy
authors.initials: W
authors.lastname: Francis
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Davies
authors.forename: Ian
authors.initials: I
authors.lastname: Pallister
authors.forename: Venkateswarlu
authors.initials: V
authors.lastname: Kanamarlapudi
authors.forename: Zhidao
authors.initials: Z
authors.lastname: Xia
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D002358:Cartilage, Articular / Q000473:pathology; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D019902:Chondrocytes / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D003907:Dexamethasone / Q000494:pharmacology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics / Q000378:metabolism*; D053222:Fas Ligand Protein / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007781:Lactoferrin / Q000494:pharmacology*; D008875:Middle Aged; D010003:Osteoarthritis / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism*
chemical_list: C491901:FAS protein, human; D053222:Fas Ligand Protein; D012333:RNA, Messenger; D019014:fas Receptor; D003907:Dexamethasone; D048049:Extracellular Signal-Regulated MAP Kinases; D007781:Lactoferrin; D053148:Caspase 3
keywords: Apoptosis; Chondrocyte; Dexamethasone; Lactoferrin; Osteoarthritis