title: Generation and characterization of a GCV resistant HCMV UL97-mutation and a drug sensitive UL54-mutation.
abstract: In transplant recipients, drug-resistant human cytomegalovirus (HCMV) infections remain a serious problem. Drug-resistance against ganciclovir (GCV), cidofovir (CDV) and foscarnet (PFA) is caused by mutations either in the phosphotransferase-gene (UL97) or in the viral polymerase (UL54). For characterization of newly emerging mutations marker transfer analysis is required. Two new HCMV-mutations, the UL54-mutation L516M and the UL97-mutation A613V, were characterized by this method.
authors.affiliation: Institute of Medical Virology and Epidemiology of Viral Diseases, University Hospital Tübingen, 72076 Tübingen, Germany.
authors.forename: Lena
authors.initials: L
authors.lastname: Fischer
authors.forename: Kerstin
authors.initials: K
authors.lastname: Laib Sampaio
authors.forename: Gerhard
authors.initials: G
authors.lastname: Jahn
authors.forename: Klaus
authors.initials: K
authors.lastname: Hamprecht
authors.forename: Katharina
authors.initials: K
authors.lastname: Göhring
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D022202:Chromosomes, Artificial, Bacterial; D000077404:Cidofovir; D003587:Cytomegalovirus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000821:virology; D003596:Cytosine / Q000031:analogs & derivatives / Q000494:pharmacology; D004259:DNA-Directed DNA Polymerase / Q000235:genetics* / Q000502:physiology; D024921:Drug Resistance, Multiple, Viral / Q000235:genetics*; D017245:Foscarnet / Q000494:pharmacology; D015774:Ganciclovir / Q000494:pharmacology*; D005814:Genes, Viral; D006801:Humans; D016296:Mutagenesis; D020125:Mutation, Missense; D063065:Organophosphonates / Q000494:pharmacology; D010641:Phenotype; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics* / Q000502:physiology; D017354:Point Mutation; D014764:Viral Proteins / Q000235:genetics* / Q000502:physiology
chemical_list: D000998:Antiviral Agents; D063065:Organophosphonates; C109367:UL54 protein, Human herpesvirus 5; D014764:Viral Proteins; D017245:Foscarnet; D003596:Cytosine; D017853:Phosphotransferases (Alcohol Group Acceptor); C075365:ganciclovir kinase; D004259:DNA-Directed DNA Polymerase; D000077404:Cidofovir; D015774:Ganciclovir
keywords: Antiviral resistance; Cidofovir; Cytomegalovirus; Foscarnet; Ganciclovir