title: MicroRNAs-449a and -449b exhibit tumor suppressive effects in retinoblastoma.
abstract: Retinoblastoma is the most common pediatric cancer of the eye. Currently, the chemotherapeutic treatments for retinoblastoma are broad-based drugs such as vincristine, carboplatin, or etoposide. However, therapies targeted directly to aberrant signaling pathways may provide more effective therapy for this disease. The purpose of our study is to illustrate the relationship between the expressions of miRs-449a and -449b to retinoblastoma proliferation and apoptosis. We are the first to confirm an inhibitory effect of miR-449a and -449b in retinoblastoma by demonstrating significantly impaired proliferation and increased apoptosis of tumor cells when these miRNAs are overexpressed. This study suggests that these miRNAs could serve as viable therapeutic targets for retinoblastoma treatment.
authors.affiliation: Division of Hematology, Oncology, and Stem Cell Transplantation, Ann and Robert H. Lurie Children's Hospital of Chicago, Chicago, IL 60611, USA.
authors.forename: Alissa
authors.initials: A
authors.lastname: Martin
authors.forename: Aunica
authors.initials: A
authors.lastname: Jones
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Bryar
authors.forename: Marilyn
authors.initials: M
authors.lastname: Mets
authors.forename: Joanna
authors.initials: J
authors.lastname: Weinstein
authors.forename: Gang
authors.initials: G
authors.lastname: Zhang
authors.forename: Nikia A
authors.initials: NA
authors.lastname: Laurie
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D058990:Molecular Targeted Therapy; D012175:Retinoblastoma / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology
chemical_list: C544530:MIRN449 microRNA, human; D035683:MicroRNAs
keywords: Apoptosis; Ct; FFPE; MicroRNA; Proliferation; RQ; Retina; Retinoblastoma; TLDA; cycle threshold; delta Ct; formalin-fixed paraffin-embedded; miRNAs; microRNAs; relative quantitation; taqman low density array; ΔCt