title: Protein carbonylation: proteomics, specificity and relevance to aging.
abstract: Detection and quantification of protein carbonyls present in biological samples has become a popular, albeit indirect, method to determine the existence of oxidative stress. Moreover, the rise of proteomics has allowed the identification of the specific proteins targeted by protein carbonylation. This review discusses these methodologies and proteomic strategies and then focuses on the relationship between protein carbonylation and aging and the parameters that may explain the increased sensitivity of certain proteins to protein carbonylation.
authors.affiliation: Departament de Ciències Mèdiques Bàsiques, IRB Lleida, Universitat de Lleida, Av. Rovira Roure, 80, 25198, Lleida, Catalonia, Spain.
authors.forename: Elisa
authors.initials: E
authors.lastname: Cabiscol
authors.forename: Jordi
authors.initials: J
authors.lastname: Tamarit
authors.forename: Joaquim
authors.initials: J
authors.lastname: Ros
mesh_terms: D000375:Aging; D000818:Animals; D006801:Humans; D018384:Oxidative Stress; D050050:Protein Carbonylation; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
chemical_list: D011506:Proteins
keywords: aging; carbonyl detection; metal-catalyzed oxidation; oxidative stress; protein carbonylation; proteomics; specific damage