title: Pollen cultivation and preparation for proteomic studies.
abstract: The quality of the collected experimental data very much depends on the quality of the biological starting material. Especially the proteome analysis of a highly dynamic system like the germinating and tube-growing pollen grain needs several precautions which allow an accurate and acceptable interpretation of the obtained results. Optimized protocols for pollen collection, storage, and in vitro culture as well as pollen organelle separations are described which help to obtain well-defined and reproducible experimental conditions for the subsequent proteomic analysis.
authors.affiliation: Molecular Plant Biophysics and Biochemistry, Department of Molecular Biology, University of Salzburg, Salzburg, Austria.
authors.forename: Heidi
authors.initials: H
authors.lastname: Pertl-Obermeyer
authors.forename: Gerhard
authors.initials: G
authors.lastname: Obermeyer
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D017360:Arabidopsis / Q000378:metabolism; D001681:Biological Assay; D002499:Centrifugation, Density Gradient; D018525:Germination; D006868:Hydrolysis; D027762:Lilium; D010940:Plant Proteins / Q000378:metabolism*; D011058:Pollen / Q000254:growth & development*; D040901:Proteomics / Q000379:methods*; D012621:Seasons; D013347:Subcellular Fractions / Q000378:metabolism; D014026:Nicotiana / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D000255:Adenosine Triphosphate