title: Anti-diabetic and antihypertensive activities of two flaxseed protein hydrolysate fractions revealed following their simultaneous separation by electrodialysis with ultrafiltration membranes.
abstract: Flaxseed protein hydrolysate has been fractionated by electrodialysis with two ultrafiltration membranes (20 and 50 kDa) stacked in the system for the recovery of two specific cationic peptide fractions (KCl-F1 and KCl-F2). After 360 min of treatment, peptide migration increased as a function of time in KCl compartments. Moreover, the use of two different ultrafiltration membrane allowed concentration of the 300-400 and 400-500 Da molecular weight range peptides in the KCl-F1 and KCl-F2 fractions, respectively, compared to the initial hydrolysate. After mass spectrometry analysis, higher amounts of low molecular weight peptides were recovered in the KCl-F2 compartment while relatively higher molecular weight peptides were more detected in the KCl-F1 compartment. Amino acid analysis showed that His, Lys and Arg were especially concentrated in the KCl compartments. Finally, glucose-transport assay demonstrated that the KCl-F2 fraction increased glucose uptake while oral administration of KCl-F1 and final FPH decreased systolic blood pressure.
authors.affiliation: Department of Food Science and Nutrition, Université Laval, Québec, Québec G1V 0A6, Canada; Institute of Nutrition and Functionnal Foods (INAF), Université Laval, Québec, Québec G1V 0A6, Canada.
authors.forename: Alain
authors.initials: A
authors.lastname: Doyen
authors.forename: Chibuike C
authors.initials: CC
authors.lastname: Udenigwe
authors.forename: Patricia L
authors.initials: PL
authors.lastname: Mitchell
authors.forename: André
authors.initials: A
authors.lastname: Marette
authors.forename: Rotimi E
authors.initials: RE
authors.lastname: Aluko
authors.forename: Laurent
authors.initials: L
authors.lastname: Bazinet
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D004553:Electric Conductivity; D019597:Flax / Q000737:chemistry*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D008297:Male; D008567:Membranes, Artificial; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D011492:Protein Hydrolysates / Q000032:analysis / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR; D014462:Ultrafiltration
chemical_list: D000596:Amino Acids; D000959:Antihypertensive Agents; D007004:Hypoglycemic Agents; D008567:Membranes, Artificial; D010455:Peptides; D011492:Protein Hydrolysates
keywords: ACE; AEM; Amino acids analysis; Antihypertensive activity; BSA; CEM; DSA; EDUF; Electrodialysis with ultrafiltration membrane; FPH; Flaxseed protein hydrolysate; Glucose uptake; HMWP; IEM; LMWP; MW; MWCO; SBP; SHR; UFM; angiotensin converting enzyme; anion-exchange membrane; bovine serum albumin; cation-exchange membrane; dimensionally-stable anode; electrodialysis with ultrafiltration membranes; flaxseed protein hydrolysate; high molecular weight peptide; ion-exchange membrane; low molecular weight peptide; molecular weight; molecular weight cut-off; spontaneously hypertensive rats; systolic blood pressure; ultrafiltration membrane