title: Effect of mesenteric lymph duct ligation on gene expression profiles of renal tissue in hemorrhagic shock rats with fluid resuscitation.
abstract: BACKGROUND
  Acute kidney injury (AKI), a common pathological process following hemorrhagic shock, can lead to an internal milieu disorder, which is an important factor of multiple organ failure (MOF). It has been shown that the mesenteric lymph return plays a deleterious effect on MOF induced by hemorrhagic shock. In this study, we investigated the effects of mesenteric lymph duct ligation (MLDL) on gene expression profiles of renal tissue following hemorrhagic shock with fluid resuscitation.
  
  
  METHODS
  After establishment of hemorrhagic shock model and fluid resuscitation in rats of shock and shock ligation groups, the MLDL was performed in shock ligation group, and only threading under the mesenteric lymph duct in the shock group. Then, the fixed position renal tissue was taken out for homogenate in two groups at 3 h after resuscitation, the total mRNA was extracted, reversely transcribed into cDNAs and marked with Cy3 and Cy5. The cDNAs were subjected for microarray scanning with 12,028 cDNA probes; the differentially expressed genes between two groups were analyzed.
  
  
  RESULTS
  In the 5812 valid dates of rat genomes transcription, there were 34 known differentially expressed genes between the two groups, of which 11 genes were up-regulated whereas 23 genes were down-regulated by MLDL. These different expressed genes encoding protein function were mainly involved in signal transduction, transcription regulation, metabolism, transport, cell growth, cell cycle, cell adhesion, cell movement, cellular component, and biological process.
  
  
  CONCLUSIONS
  The mechanism of MLDL alleviating the AKI aftershock might be associated with up- or down-regulation of the above gene expressions.
authors.affiliation: Institute of Cell Biology, Zhejiang University , Hangzhou, Zhejiang , PR China .
authors.forename: Zi-Gang
authors.initials: ZG
authors.lastname: Zhao
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Niu
authors.forename: Jing-Fu
authors.initials: JF
authors.lastname: Qiu
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Chen
authors.forename: Ji-Cheng
authors.initials: JC
authors.lastname: Li
mesh_terms: D058186:Acute Kidney Injury / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D018076:DNA, Complementary / Q000032:analysis; D005440:Fluid Therapy; D015870:Gene Expression; D007668:Kidney / Q000378:metabolism*; D008026:Ligation; D042601:Lymphatic Vessels / Q000378:metabolism*; D008643:Mesentery; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000032:analysis; D051381:Rats; D012771:Shock, Hemorrhagic / Q000378:metabolism* / Q000628:therapy
chemical_list: D018076:DNA, Complementary; D012313:RNA