title: Hormonal pretreatment preserves liver regenerative capacity and minimizes inflammation after partial hepatectomy.
abstract: BACKGROUND
  The treatment of brain dead donors with combined hormonal resuscitation protocols, including methylprednisolone (MP) and triiodothyronine (T3), among others, was developed to increase the viability and function of transplanted organs, primarily heart and lung. Even when it has regarded successful results in term of donors and organs recovery, its effects over specific parameters in organs like the liver are unknown.
  
  
  METHODS
  Male Sprague-Dawley rats were pretreated with MP (0.34 mg/kg) and/or T3 (0.05 mg/kg) or their vehicles, and then subjected to partial hepatectomy of 70%. Three experimental groups and their respective controls were conformed: a. T3; b. NaOH; c. MP; d. vMP; e. MP+T3 and f. vMP+NaOH. The groups were evaluated at 0, 16, 24, 72 and 120 h post surgery. The effects of this protocol on regeneration, liver mass recovery, liver injury, oxidative stress and liver function were analyzed.
  
  
  RESULTS
  MP+T3 pretreatment does not deleteriously affect liver regeneration after partial hepatectomy, as shown in the curve of total mass recovery, Ki67 staining and mitosis counting, and does not alter liver function. In addition, the treatment modestly decreases oxidative stress and liver injury, as evidenced by transaminases levels, histological analysis and oxidized proteins content.
  
  
  CONCLUSIONS
  These preclinical results indicate that MP+T3 is harmless for liver tissue regeneration post hepatectomy and additionally exhibits anti-inflammatory and antioxidant effects; therefore, it would not be contraindicated for the treatment of multiorgan donors in brain death and particularly, if the occurrence of small for size syndrome is suspected.
authors.affiliation: Facultad de Ciencias, Universidad de Chile.
authors.forename: Amanda
authors.initials: A
authors.lastname: D'Espessailles
authors.forename: Camila
authors.initials: C
authors.lastname: Dossi
authors.forename: Giomar
authors.initials: G
authors.lastname: Intriago
authors.forename: Pedro
authors.initials: P
authors.lastname: Leiva
authors.forename: Pamela
authors.initials: P
authors.lastname: Romanque
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D006498:Hepatectomy / Q000009:adverse effects; D006505:Hepatitis / Q000209:etiology / Q000517:prevention & control*; D019394:Ki-67 Antigen / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D008775:Methylprednisolone / Q000494:pharmacology*; D008940:Mitotic Index; D009336:Necrosis; D018384:Oxidative Stress / Q000187:drug effects; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014284:Triiodothyronine / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D019394:Ki-67 Antigen; D014284:Triiodothyronine; D008775:Methylprednisolone