title: Assessment of free radicals scavenging activity of seven natural pigments and protective effects in AAPH-challenged chicken erythrocytes.
abstract: The purpose of this study was to compare the antioxidant capacities of seven natural pigments including the fat-soluble pigments curcumin, lycopene, lutein and β-carotene and water-soluble pigments--betalain, capsanthin and cyanidin-3-rutinoside relative to a commonly-used synthetic food antioxidant BHA. The antioxidant capacities of seven pigments and BHA were evaluated based on their ability to quench several free radicals, including DPPH, ABTS, O2(·-), H2O2 as well as using FRAP assay. Specifically, curcumin and cyanidin-3-rutinoside, which showed the highest antioxidant capacities, were further investigated using a chicken erythrocyte model. After separating pretreatments of the two pigments, AAPH was added to the erythrocyte-pigment medium to induce oxidative stress. Then the attenuation effects of the two pigments on AAPH-induced oxidative damage in chicken erythrocytes were assessed. It was found that both curcumin and cyanidin-3-rutinoside significantly attenuated apoptosis and hemolysis, decreased MDA content, and increased T-SOD activity in a time- and dose- dependent manner.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Jingfei
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiang
authors.initials: X
authors.lastname: Hou
authors.forename: Hussain
authors.initials: H
authors.lastname: Ahmad
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.forename: Tian
authors.initials: T
authors.lastname: Wang
mesh_terms: D000578:Amidines / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002645:Chickens; D003474:Curcumin / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D010860:Pigments, Biological / Q000494:pharmacology*
chemical_list: D000578:Amidines; D000975:Antioxidants; D016166:Free Radical Scavengers; D010860:Pigments, Biological; C046728:2,2'-azobis(2-amidinopropane); D003474:Curcumin
keywords: Apoptosis; Curcumin; Cyanidin-3-rutinoside; Free radicals; Hemolysis; Lipid peroxidation