title: Expression and function of Allergin-1 on human primary mast cells.
abstract: Mast cells (MC) play an important role in allergic and non-allergic immune responses. Activation of human MC is modulated by several cell surface inhibitory receptors, including recently identified Allergin-1 expressed on both human and mouse MC. Although Allergin-1 suppresses IgE-mediated, mast cell-dependent anaphylaxis in mice, the expression profile and function of Allergin-1 on human primary MC remains undetermined. Here, we established a seven-color flow cytometry method for assessing expression and function of a very small number of human primary MC. We show that Allergin-1S1, a splicing isoform of Allergin-1, is predominantly expressed on human primary MC in both bronchoalveolar lavage (BAL) fluid and nasal scratching specimens. Moreover, Allergin-1S1 inhibits IgE-mediated activation from human primary MC in BAL fluid. These results indicate that Allergin-1 on human primary MC exhibits similar characteristics as mouse Allergin-1 in the expression profile and function.
authors.affiliation: Department of Immunology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan ; Department of Nephrology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan ; Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Tokyo, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Nagai
authors.forename: Satoko
authors.initials: S
authors.lastname: Tahara-Hanaoka
authors.forename: Yuko
authors.initials: Y
authors.lastname: Morishima
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tokunaga
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Imoto
authors.forename: Emiko
authors.initials: E
authors.lastname: Noguchi
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Kanemaru
authors.forename: Masamichi
authors.initials: M
authors.lastname: Imai
authors.forename: Shiro
authors.initials: S
authors.lastname: Shibayama
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hizawa
authors.forename: Shigeharu
authors.initials: S
authors.lastname: Fujieda
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Yamagata
authors.forename: Akira
authors.initials: A
authors.lastname: Shibuya
mesh_terms: D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism; D008407:Mast Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*
chemical_list: D011971:Receptors, Immunologic; D007073:Immunoglobulin E