title: [Transcription activator-like effectors(TALEs)based genome engineering].
abstract: Systematic reverse-engineering of functional genome architecture requires precise modifications of gene sequences and transcription levels. The development and application of transcription activator-like effectors(TALEs) has created a wealth of genome engineering possibilities. TALEs are a class of naturally occurring DNA-binding proteins found in the plant pathogen Xanthomonas species. The DNA-binding domain of each TALE typically consists of tandem 34-amino acid repeat modules rearranged according to a simple cipher to target new DNA sequences. Customized TALEs can be used for a wide variety of genome engineering applications, including transcriptional modulation and genome editing. Such "genome engineering" has now been established in human cells and a number of model organisms, thus opening the door to better understanding gene function in model organisms, improving traits in crop plants and treating human genetic disorders.
authors.affiliation: College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China. chengliduan@hotmail.com; liujiang@mail.kiz.ac.cn.
authors.forename: Mei-Wei
authors.initials: MW
authors.lastname: Zhao
authors.forename: Cheng-Li
authors.initials: CL
authors.lastname: Duan
authors.forename: Jiang
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D005818:Genetic Engineering / Q000379:methods*; D016678:Genome; D006801:Humans; D011995:Recombination, Genetic; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D015534:Trans-Activators
keywords: Double-strand break; Genome editing; Homologous recombination; Non-homologous end joining; TALEs; Transcriptional modulation