title: Designer cells for stereocomplementary de novo enzymatic cascade reactions based on laboratory evolution.
abstract: Designer cells for a synthetic cascade reaction harnessing selective redox reactions were devised, featuring two successive regioselective P450-catalyzed CH-activating oxidations of 1-cyclohexene carboxylic acid methyl ester followed by stereoselective olefin-reduction catalysed by (R)- or (S)-selective mutants of an enoate reductase.
authors.affiliation: Fachbereich Chemie, Philipps-Universität Marburg, Hans-Meerwein Str., 35032 Marburg, Germany.
authors.forename: Rubén
authors.initials: R
authors.lastname: Agudo
authors.forename: Manfred T
authors.initials: MT
authors.lastname: Reetz
mesh_terms: D000475:Alkenes / Q000737:chemistry; D001410:Bacillus megaterium / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D055162:Biocatalysis; D003509:Cyclohexanecarboxylic Acids / Q000737:chemistry; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D013237:Stereoisomerism
chemical_list: D000475:Alkenes; D001426:Bacterial Proteins; D003509:Cyclohexanecarboxylic Acids; D011994:Recombinant Proteins; C074886:1-cyclohexenecarboxylic acid; D003577:Cytochrome P-450 Enzyme System