title: Characterization of grass carp (Ctenopharyngodon idella) IL-17D: molecular cloning, functional implication and signal transduction.
abstract: Although the roles of IL-17 family members during inflammation have been extensively studied in mammals, their knowledge in lower vertebrates is limited. In particular, the biological activities of fish IL-17 and their functional roles are largely unknown. In this study, we cloned grass carp IL-17D (gcIL-17D) and found that its putative protein possessed the conserved features of IL-17 family members. Tissue distribution analysis showed that gcIL-17D was preferentially expressed in the mucosal tissues, including skin, gill and intestine. Subsequently, the involvement of gcIL-17D in inflammatory response was demonstrated by examining the expression profiles of gcIL-17D in head kidney and head kidney leukocytes following in vivo bacterial infection and in vitro LPS treatment, respectively. Furthermore, recombinant gcIL-17D (rgcIL-17D) was prepared in grass carp kidney cells and was able to promote the gene expression of some pro-inflammatory cytokines (IL-1β, TNF-α and CXCL-8) in grass carp primary head kidney cells, revealing gcIL-17D can function as a pro-inflammatory cytokine. Moreover, rgcIL-17D appeared to activate NF-κB signaling by modulating the phosphorylation of IκBα and up-regulated CXCL-8 mRNA expression possibly through NF-κB pathway. Our data shed new light on the functional role of teleost IL-17D in inflammatory response.
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, People's Republic of China.
authors.forename: Linyong
authors.initials: L
authors.lastname: Du
authors.forename: Lei
authors.initials: L
authors.lastname: Qin
authors.forename: Xinyan
authors.initials: X
authors.lastname: Wang
authors.forename: Anying
authors.initials: A
authors.lastname: Zhang
authors.forename: He
authors.initials: H
authors.lastname: Wei
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology; D029941:Fish Proteins / Q000235:genetics; D020869:Gene Expression Profiling; D005880:Gills / Q000276:immunology; D016905:Gram-Negative Bacterial Infections / Q000276:immunology; D060226:Head Kidney / Q000166:cytology / Q000276:immunology; D020672:I-kappa B Proteins / Q000378:metabolism; D007249:Inflammation / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D053583:Interleukin-1beta / Q000096:biosynthesis; D064094:Interleukin-27 / Q000235:genetics* / Q000276:immunology* / Q000493:pharmacokinetics; D016209:Interleukin-8 / Q000096:biosynthesis / Q000235:genetics; D007413:Intestinal Mucosa / Q000276:immunology; D007962:Leukocytes / Q000276:immunology; D008070:Lipopolysaccharides; D008969:Molecular Sequence Data; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000276:immunology; D010766:Phosphorylation; D012333:RNA, Messenger / Q000096:biosynthesis; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D012867:Skin / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D020672:I-kappa B Proteins; D053583:Interleukin-1beta; D064094:Interleukin-27; D016209:Interleukin-8; D008070:Lipopolysaccharides; D016328:NF-kappa B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D000072000:NF-KappaB Inhibitor alpha
keywords: Bioactivity; Expression analysis; Grass carp; IL-17D; Signal transduction