title: Ewing sarcoma cells secrete EWS/Fli-1 fusion mRNA via microvesicles.
abstract: Tumours defined as Ewing sarcoma (ES) constitute a group of highly malignant neoplasms that most often affect children and young adults in the first 2 decades of life. The EWS/Fli-1 fusion gene, a product of the translocation t(11;22) (q24; 12), is detected in 95% of ES patients. Recently, it was validated that cells emit a heterogeneous mixture of vesicular, organelle-like structures (microvesicles, MVs) into their surroundings including blood and body fluids, and that these MVs contain a selected set of tumor-related proteins and high levels of mRNAs and miRNAs. In this present study, we detected the Ewing sarcoma-specific EWS/Fli-1 mRNA in MVs from the culture medium of ES cell lines carrying t(11;22) (q24; 12). Also, we detected this fusion gene in approximately 40% of the blood samples from mice inoculated with xenografts of TC135 or A673 cells. These findings indicate the EWS/Fli-1 mRNA in MVs might be a new non-invasive diagnostic marker for specific cases of Ewing sarcoma.
authors.affiliation: Department of Orthopaedic Surgery, Gifu University Graduate School of Medicine, Gifu, Gifu, Japan ; United Graduate School of Drug Discovery and Medical Information Sciences, Gifu, Gifu, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Tsugita
authors.forename: Nami
authors.initials: N
authors.lastname: Yamada
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Noguchi
authors.forename: Kazunari
authors.initials: K
authors.lastname: Yamada
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Moritake
authors.forename: Katsuji
authors.initials: K
authors.lastname: Shimizu
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Akao
authors.forename: Takatoshi
authors.initials: T
authors.lastname: Ohno
mesh_terms: D000818:Animals; D001483:Base Sequence; D001692:Biological Transport; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005110:Extracellular Space / Q000378:metabolism; D064593:Heterografts; D006801:Humans; D051379:Mice; D015514:Oncogene Proteins, Fusion / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D051789:Proto-Oncogene Protein c-fli-1 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D034802:RNA-Binding Protein EWS / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D012512:Sarcoma, Ewing / Q000235:genetics* / Q000378:metabolism*; D022142:Secretory Vesicles / Q000378:metabolism*
chemical_list: C106031:EWS-FLI fusion protein; D015514:Oncogene Proteins, Fusion; D051789:Proto-Oncogene Protein c-fli-1; D012333:RNA, Messenger; D034802:RNA-Binding Protein EWS