title: Proteolytic characterisation in grass carp sausage inoculated with Lactobacillus plantarum and Pediococcus pentosaceus.
abstract: The proteolysis in grass carp sausages inoculated with Lactobacillus plantarum ZY40 and Pediococcus pentosaceus GY23 was investigated. As fermentation progressed, sarcoplasmic and myofibrillar proteins in both sausages were obviously degraded, and the proteolytic process was more intense in sausages inoculated with P. pentosaceus GY23. The increases in α-amino nitrogen, trichloroacetic acid (TCA)-soluble peptides and free amino acids were also detected in both sausages. The differences in α-amino nitrogen content and free amino acids concentration were due to the activity of inoculated lactic acid bacteria, while endogenous enzymes contributed to the release of TCA-soluble peptides. Our findings indicate that lactic acid bacteria influence proteolytic characterisation in fermented fish sausage, with strain-dependent activity.
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China. Electronic address: niexiaohua2000@126.com.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Nie
authors.forename: Shengli
authors.initials: S
authors.lastname: Lin
authors.forename: Qilin
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000596:Amino Acids / Q000032:analysis / Q000378:metabolism; D000818:Animals; D002347:Carps; D002681:China; D004032:Diet / Q000208:ethnology; D004044:Dietary Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D005396:Fish Products / Q000032:analysis* / Q000382:microbiology; D029941:Fish Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D061353:Food Storage; D057141:Food, Preserved / Q000032:analysis* / Q000382:microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D048191:Lactiplantibacillus plantarum / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D009124:Muscle Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D010376:Pediococcus / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis / Q000378:metabolism; D059748:Proteolysis; D017673:Sodium Chloride, Dietary / Q000032:analysis; D013045:Species Specificity
chemical_list: D000596:Amino Acids; D004044:Dietary Proteins; D029941:Fish Proteins; D009124:Muscle Proteins; D010446:Peptide Fragments; D017673:Sodium Chloride, Dietary
keywords: Grass carp sausage; Lactobacillus plantarum; Pediococcus pentosaceus; Proteolysis