title: Near-infrared fluorescence energy transfer imaging of nanoparticle accumulation and dissociation kinetics in tumor-bearing mice.
abstract: In the current study we show the dissociation and tumor accumulation dynamics of dual-labeled near-infrared quantum dot core self-assembled lipidic nanoparticles (SALNPs) in a mouse model upon intravenous administration. Using advanced in vivo fluorescence energy transfer imaging techniques, we observed swift exchange with plasma protein components in the blood and progressive SALNP dissociation and subsequent trafficking of individual SALNP components following tumor accumulation. Our results suggest that upon intravenous administration SALNPs quickly transform, which may affect their functionality. The presented technology provides a modular in vivo tool to visualize SALNP behavior in real time and may contribute to improving the therapeutic outcome or molecular imaging signature of SALNPs.
authors.affiliation: Condensed Matter and Interfaces, Debye Institute for Nanomaterials Science, Utrecht University , Princetonplein 5, 3584 CC Utrecht, The Netherlands.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Zhao
authors.forename: Inge
authors.initials: I
authors.lastname: van Rooy
authors.forename: Sjoerd
authors.initials: S
authors.lastname: Hak
authors.forename: Francois
authors.initials: F
authors.lastname: Fay
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.forename: Catharina de Lange
authors.initials: Cde L
authors.lastname: Davies
authors.forename: Mihaela
authors.initials: M
authors.lastname: Skobe
authors.forename: Edward Allen
authors.initials: EA
authors.lastname: Fisher
authors.forename: Aurelian
authors.initials: A
authors.lastname: Radu
authors.forename: Zahi A
authors.initials: ZA
authors.lastname: Fayad
authors.forename: Celso
authors.initials: C
authors.lastname: de Mello Donegá
authors.forename: Andries
authors.initials: A
authors.lastname: Meijerink
authors.forename: Willem J M
authors.initials: WJ
authors.lastname: Mulder
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D006801:Humans; D007700:Kinetics; D008055:Lipids / Q000737:chemistry; D051379:Mice; D008823:Micelles; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging; D053758:Nanoparticles / Q000032:analysis* / Q000737:chemistry; D036103:Nanotechnology; D009368:Neoplasm Transplantation; D055095:Optics and Photonics; D045663:Quantum Dots
chemical_list: D008055:Lipids; D008823:Micelles