title: Protein expression profiling during wallerian degeneration after rat sciatic nerve injury.
abstract: BACKGROUND
  Wallerian degeneration (WD) is an important area of research in modern neuroscience. Many protein expressions are regulated by differentially expressed genes in WD, but the precise mechanisms are elusive.
  
  
  METHODS
  In this study, we profiled differentially expressed proteins in WD after rat sciatic nerve injury using an antibody array.
  
  
  RESULTS
  Functional analysis positively identified cell proliferation, regulation of cell proliferation, and immune system processes. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed molecular networks related mainly to cytokine-cytokine receptor interaction, the mitogen-activated proteinkinase (MAPK) signaling pathway, apoptosis, the toll-like receptor (TLR) signaling pathway, and the Janus kinase (Jak) - signal transducer and activator of transcription (STAT) signaling pathway. Interactions between these differential proteins were well established and regulated by the key factors transforming growth factor beta 1 (TGF-β1), toll-like receptor 4 (TLR4), Fas ligand (FasL), and 5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1).
  
  
  CONCLUSIONS
  These results provide information related to functional analysis of differentially expressed genes during WD.
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu, P.R. China.
authors.forename: Meiyuan
authors.initials: M
authors.lastname: Li
authors.forename: Pingan
authors.initials: P
authors.lastname: Zhang
authors.forename: Weimin
authors.initials: W
authors.lastname: Guo
authors.forename: Huaiqin
authors.initials: H
authors.lastname: Li
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Gu
authors.forename: Dengbing
authors.initials: D
authors.lastname: Yao
mesh_terms: D000818:Animals; D001710:Biotin; D015153:Blotting, Western; D016000:Cluster Analysis; D019295:Computational Biology; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D008297:Male; D009410:Nerve Degeneration / Q000235:genetics; D009416:Nerve Regeneration / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D012584:Sciatic Nerve / Q000293:injuries*; D015398:Signal Transduction / Q000502:physiology; D014855:Wallerian Degeneration / Q000235:genetics*
chemical_list: D001710:Biotin
keywords: Wallerian degeneration; antibody arrays; bioinformatic analysis; protein expression; rat; sciatic nerve