title: Protective effects of fish omega-3 fatty acids on doxorubicin-induced testicular apoptosis and oxidative damage in rats.
abstract: The aim of this study was to examine the protective effects of fish omega-3 (n-3) fatty acids on acute doxorubicin (DOX)-induced testicular apoptosis and oxidative damage. 24 male rats were divided into three groups: control, DOX-treated and DOX+fish n-3 fatty acids. Fish n-3 fatty acids (400 mg kg(-1) ) were given for 30 days by intragastric gavage. The rats received a single intraperitoneal injection of DOX (30 mg kg(-1) ) and were sacrificed after 48 h. The DOX+fish n-3 fatty acids group showed a decrease in malondialdehyde levels and increased activities of superoxide dismutase and glutathione peroxidase in comparison with the DOX-treated group. Acute DOX treatment caused severe damage such as disorganisation and separation of germ cells. The fish n-3 fatty acids-pretreated rats showed an improved histological appearance in the DOX-treated group. Our data indicate a reduction in the activity of terminal deoxynucleotidyl transferase mediated dUTP nick end labelling; there was a rise in the expression of proliferating cell nuclear antigen in testis tissues of the DOX+fish n-3 fatty acids group compared with DOX-treated group. These data suggested that fish n-3 fatty acids pre-treatment may be beneficial for spermatogenesis following acute DOX-induced testicular damage by decreasing germ cell apoptosis and oxidative stress.
authors.affiliation: Faculty of Medicine, Department of Anatomy, Namik Kemal University, Tekirdag, Turkey.
authors.forename: R
authors.initials: R
authors.lastname: Uygur
authors.forename: C
authors.initials: C
authors.lastname: Aktas
authors.forename: F
authors.initials: F
authors.lastname: Tulubas
authors.forename: E
authors.initials: E
authors.lastname: Uygur
authors.forename: M
authors.initials: M
authors.lastname: Kanter
authors.forename: M
authors.initials: M
authors.lastname: Erboga
authors.forename: V
authors.initials: V
authors.lastname: Caglar
authors.forename: B
authors.initials: B
authors.lastname: Topcu
authors.forename: O A
authors.initials: OA
authors.lastname: Ozen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004317:Doxorubicin; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D013733:Testicular Diseases / Q000139:chemically induced / Q000517:prevention & control*
chemical_list: D015525:Fatty Acids, Omega-3; D004317:Doxorubicin
keywords: Apoptosis; doxorubicin; fish omega-3 fatty acids; proliferating cell nuclear antigen; testis