title: Affinity adsorption of lysozyme with Reactive Red 120 modified magnetic chitosan microspheres.
abstract: Reactive Red 120 was utilized as an affinity dye-ligand to modify the surface of magnetic chitosan microspheres to improve the adsorption capacity for lysozyme. The impact of pH, ionic strength, adsorption time and initial lysozyme concentration on the adsorption of lysozyme from aqueous solutions was investigated. An increased adsorption amount of 116.9 mg/g lysozyme on Reactive Red 120 modified microspheres was achieved in comparison to 24.6 mg/g on unmodified magnetic chitosan microspheres. The adsorption behaviour of lysozyme by the Reactive Red 120 modified magnetic chitosan microspheres fitted the pseudo second-order kinetic and the Langmuir thermodynamic model. The desorption ratio was 92.6% using 1M NaCl phosphate buffer (pH 6.0) as the desorption agent. The adsorption capacity of the Reactive Red 120 modified magnetic chitosan microspheres did not decrease significantly after four adsorption-desorption cycles (P>0.05). The as-prepared Reactive Red 120 modified magnetic chitosan microspheres were then used for the extraction of lysozyme from egg white solutions. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) results showed that the purity of the obtained lysozyme was ~80.7% and that the recovery yield was ~89.1%.
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address: steveli@nwsuaf.edu.cn.
authors.forename: Zhonghong
authors.initials: Z
authors.lastname: Li
authors.forename: Miao
authors.initials: M
authors.lastname: Cao
authors.forename: Wengang
authors.initials: W
authors.lastname: Zhang
authors.forename: Lizhi
authors.initials: L
authors.lastname: Liu
authors.forename: Jianlong
authors.initials: J
authors.lastname: Wang
authors.forename: Wupeng
authors.initials: W
authors.lastname: Ge
authors.forename: Yahong
authors.initials: Y
authors.lastname: Yuan
authors.forename: Tianli
authors.initials: T
authors.lastname: Yue
authors.forename: Ronghua
authors.initials: R
authors.lastname: Li
authors.forename: William W
authors.initials: WW
authors.lastname: Yu
mesh_terms: D000327:Adsorption; D048271:Chitosan / Q000737:chemistry*; D004529:Egg White; D004591:Electrophoresis, Polyacrylamide Gel; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008280:Magnetics; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008863:Microspheres; D009113:Muramidase / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D009994:Osmolar Concentration; D013816:Thermodynamics; D014227:Triazines / Q000737:chemistry*
chemical_list: D058185:Magnetite Nanoparticles; D014227:Triazines; C042516:reactive red 120 dye; D048271:Chitosan; D009113:Muramidase; D052203:Ferrosoferric Oxide
keywords: Adsorption; Egg white; Lysozyme; Magnetic microspheres; Reactive Red 120