title: Photoactivation of a psoralen-blocked luciferase gene by blue light.
abstract: A single psoralen cross-link completely blocks expression of a gene. An aminopyrene derivative has been found that allows the efficient light-triggered activation of cross-linked genes by UV or blue light. This paves the way to apply such psoralen cross-links for the photocleavable protection of nucleic acids.
authors.affiliation: Interfaculty Institute of Biochemistry, University of Tübingen, Auf der Morgenstelle 15, 72076 Tübingen (Germany) http://www.ifib.uni-tuebingen.de/forschung/stafforst.html. thorsten.stafforst@uni-tuebingen.de.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Stafforst
authors.forename: Jens M
authors.initials: JM
authors.lastname: Stadler
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D018736:DNA Adducts / Q000737:chemistry; D004579:Electron Transport; D005363:Ficusin / Q000737:chemistry*; D008027:Light; D008156:Luciferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020011:Protective Agents / Q000737:chemistry / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D018736:DNA Adducts; D020011:Protective Agents; D004247:DNA; D008156:Luciferases; D005363:Ficusin
keywords: electron transfer; nucleic acids; photoactivation; photochemistry; pyrene