title: Novel antioxidative peptides from the protein hydrolysate of oysters (Crassostrea talienwhanensis).
abstract: The antioxidative activity of hydrolysate peptides from oysters (Crassostrea talienwhanensis) was investigated. After hydrolysis with subtilisin, the yields of the peptides that were soluble in trichloroacetic acid (TCA-soluble) and the antioxidant activities of the resulting hydrolysate were determined using an orthogonal design and a hydroxyl radical scavenging reaction. The hydrolysate was fractionated using Sephadex G-15 gel filtration chromatography, and the two resulting bioactive peptides were subsequently purified by RP-HPLC with a Kromasil C18 (ODS) column. The amino acid sequences were analyzed by nano-ESI-MS/MS. The critical reaction temperature, pH, hydrolysis time and enzyme-to-substrate (E/S) ratio were determined for the optimum hydrolysis with subtilisin, and the E/S ratio was found to be the most critical reaction condition. The amino acid sequences of the peptides (518 and 440 Da) were proline-valine-methionine-glycine-aspartic acid (PVMGA) and glutamine-histidine-glycine-valine (QHGV), respectively. These two novel peptides exhibited high antioxidative actions based on their hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities.
authors.affiliation: Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China; Key Laboratory of Aquatic Products Processing and Utilization of Liaoning Province, Dalian Ocean University, Dalian 116023, PR China.
authors.forename: Qiukuan
authors.initials: Q
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Yunhai
authors.initials: Y
authors.lastname: He
authors.forename: Dandan
authors.initials: D
authors.lastname: Ren
authors.forename: Felicia
authors.initials: F
authors.lastname: Kow
authors.forename: Linlin
authors.initials: L
authors.lastname: Song
authors.forename: Xingju
authors.initials: X
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002681:China; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D049896:Crassostrea / Q000737:chemistry*; D004044:Dietary Proteins / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D019587:Dietary Supplements; D016166:Free Radical Scavengers / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors; D008832:Microchemistry; D009842:Oligopeptides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011492:Protein Hydrolysates / Q000737:chemistry / Q000378:metabolism; D012758:Shellfish / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization; D020860:Subtilisin / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D004044:Dietary Proteins; D016166:Free Radical Scavengers; D009842:Oligopeptides; D010446:Peptide Fragments; D011492:Protein Hydrolysates; C000588967:glutaminyl-histidyl-glycyl-valine; C000588966:prolyl-valyl-methionyl-glycyl-aspartic acid; D017665:Hydroxyl Radical; D020860:Subtilisin
keywords: Antioxidative peptide; Hydrolysate; Oyster Crassostrea talienwhanensis; Radical scavenging activity; Subtilisin