title: Simultaneous determination of selected chemotherapeutics in human whole blood by molecularly imprinted polymers coated solid phase microextraction fibers and liquid chromatography-tandem mass spectrometry.
abstract: Development and validation of novel, general liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous determination of amoxicillin (AMOX), cefatoxime (CEF), ciprofloxacin (CIP), daptomycin (DAPTO), fluconazole (FLU), gentamicin (GEN), clindamycin (KLI), linezolid (LIN), metronidazole (MET), moxifloxacin (MOXI) in human whole blood are described. Samples were prepared on solid phase microextraction way with the use of polymeric sorption coatings with molecular imprints and analyzed using a gradient separation over an ACE C18-column (4.6mm×150mm, 3μm) with isocratic elution. Positive electrospray ionization was employed as the ionization source. The mobile phase consisted of acetonitrile-water (0.1% formic acid or 5mM ammonium acetate) at a flow 0.4ml/min. The chromatographic run time was kept less than 9min. The intra- and inter-day relative standard deviation across three validation runs over the entire concentration range was less than 7.3%, while the accuracy was within ±8.4%. The mean recovery of all the analytes ranged from 65.0 to 83.0%. This method was successfully applied to clinical samples from patients with clinically diagnosed bacterial infections process.
authors.affiliation: Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7 Street, 87-100 Torun, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Szultka
authors.forename: Rafal
authors.initials: R
authors.lastname: Krzeminski
authors.forename: Marek
authors.initials: M
authors.lastname: Jackowski
authors.forename: Boguslaw
authors.initials: B
authors.lastname: Buszewski
mesh_terms: D000368:Aged; D000900:Anti-Bacterial Agents / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use; D001424:Bacterial Infections / Q000188:drug therapy; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D016018:Least-Squares Analysis; D057230:Limit of Detection; D008875:Middle Aged; D054802:Molecular Imprinting / Q000379:methods*; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction / Q000295:instrumentation* / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antibiotic drugs; Liquid chromatography–mass spectrometry; Molecular imprinted polymers; Solid phase microextraction; Whole human blood