title: Adiponectin and p53 mRNA in epicardial and subcutaneous fat from heart failure patients.
abstract: OBJECTIVE
  Heart failure (HF) is associated with a pro-inflammatory state in epicardial fat, but the involved mechanisms are not entirely clear. The aim of our study was to assess the relationship between p53 and adiponectin mRNA in epicardial adipose tissue (EAT) and subcutaneous adipose tissue (SAT) in patients with heart failure and its sympathetic regulation.
  
  
  METHODS
  Epicardial adipose tissue and SAT samples were obtained from 63 patients undergoing elective cardiac surgery. EAT and SAT explants culture from seven patients were stimulated with isoprenaline 0.1 or 1 uM for 6 h. p53 and adiponectin mRNA expression was measured in frozen biopsies or explants culture from both fat pads by real-time polymerase chain reaction (PCR).
  
  
  RESULTS
  We observed that EAT expressed more p53 mRNA than SAT (1.73 ± 0.07 vs. 1.69 ± 0.04, P < 0.001) and its levels were higher in HF patients (1.75 ± 0.07 vs. 1.70 ± 0.04, P < 0.01 in EAT and 1.70 ± 0.04 vs. 1.67 ± 0.04, P < 0.05 in SAT). Moreover, p53 mRNA expression was negatively correlated with adiponectin in EAT. After analysing the p53 mRNA regulation by isoprenaline, we observed that only EAT p53 expression increased after adrenergic stimulation (1.63 ± 0.01 vs. 1.66 ± 0.02; P = 0.024).
  
  
  CONCLUSIONS
  p53 mRNA expression levels, inversely correlated with adiponectin, increase in EAT of HF patients and can be regulated by sympathetic activation pathway. Our findings can help to explain the deleterious effect of sympathetic activation in HF.
authors.affiliation: Department of Cardiology and Coronary Unit, University Clinical Hospital of Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Agra
authors.forename: Elvis
authors.initials: E
authors.lastname: Teijeira-Fernández
authors.forename: Domingo
authors.initials: D
authors.lastname: Pascual-Figal
authors.forename: Jesús
authors.initials: J
authors.lastname: Sánchez-Más
authors.forename: Angel
authors.initials: A
authors.lastname: Fernández-Trasancos
authors.forename: José R
authors.initials: JR
authors.lastname: González-Juanatey
authors.forename: Sonia
authors.initials: S
authors.lastname: Eiras
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D052242:Adiponectin / Q000235:genetics* / Q000378:metabolism; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006333:Heart Failure / Q000378:metabolism*; D006801:Humans; D050152:Intra-Abdominal Fat / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D010496:Pericardium; D012333:RNA, Messenger / Q000032:analysis* / Q000187:drug effects; D060888:Real-Time Polymerase Chain Reaction; D017154:Stromal Cells / Q000378:metabolism; D050151:Subcutaneous Fat / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
chemical_list: D052242:Adiponectin; D000318:Adrenergic beta-Agonists; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D007545:Isoproterenol
keywords: Adiponectin; epicardial fat; p53; sympathetic activation and heart failure