title: In vitro evaluation of potential complexation between bovine insulin and bovine serum albumin.
abstract: The objective of this study was to examine the possible binding of bovine insulin (BI) with bovine serum albumin (BSA) to form a new potential diabetogenic irreversible complex protein. Several preparations of BSA and BI were prepared. Both capillary electrophoresis and spectrophotometric analysis were undertaken to test the possibility of complexation between BI and BSA. HPLC was used to test whether the potential complex of BI and BSA is reversible or irreversible. The optimum deviation between the real and calculated absorbances was observed at a BI/BSA ratio of 2. Moreover, the migration time of BI decreased substantially with increasing ratio of BI to BSA until it became almost constant at equal molar ratio of BI/BSA. While the majority of the 2:1 BI-BSA sample detached during the HPLC analysis, which confirms the reversible character of BI-BSA binding, the HPLC chromatogram also emphasizes the formation of an irreversible complexation between the two proteins. This study provides evidence of the formation of reversible and irreversible new BI-BSA complexes under physiological conditions. This highlights the importance of examining the possible diabetogenicity of BI-BSA complex in genetically susceptible people.
authors.affiliation: Department of Nutrition and Food Technology, Faculty of Agriculture, The University of Jordan, Amman, 11942, Jordan.
authors.forename: Hayder
authors.initials: H
authors.lastname: Al-Domi
authors.forename: Muhammed
authors.initials: M
authors.lastname: Alzweiri
authors.forename: Imad
authors.initials: I
authors.lastname: Hamdan
authors.forename: Ziad
authors.initials: Z
authors.lastname: Jaradat
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D061385:Insulins / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
chemical_list: D061385:Insulins; D012710:Serum Albumin, Bovine
keywords: bovine albumin; bovine insulin; diabetogenicity; dietary