title: Mamestra configurata nucleopolyhedrovirus-A transcriptome from infected host midgut.
abstract: Infection of an insect by a baculovirus occurs in two distinct phases, an initial infection of host midgut by occlusion-derived virions (ODVs) and subsequent systemic infection of other tissues by budded virions (BV). A vast majority of investigations of the infection process have been restricted to cell culture studies using BV that emulate the systemic phase of infection. This is one of the first studies to investigate baculovirus gene expression in ODV infected midgut cells. We have focused on the critical first phase of in vivo infection by Mamestra configurata nucleopolyhedrovirus-A in M. configurata larvae, using qPCR and RNAseq mass sequencing to measure virus gene expression in midgut cells. The earliest genes detected by each method had significant overlap, including known early genes as well as genes unique to MacoNPV-A and genes of unknown function. The RNAseq data also revealed a large range of expression levels across all ORFs, which could not be measured using qPCR. This dataset provides a first whole genome transcriptomic analysis of viral genes required for virus infection in vivo and will provide the basis for functionally analyzing specific genes that may be critical elements in baculovirus midgut infectivity and host range.
authors.affiliation: Southern Crop Protection & Food Research Centre, AAFC, London, ON, N5V 4T3, Canada, cam.donly@agr.gc.ca.
authors.forename: B Cameron
authors.initials: BC
authors.lastname: Donly
authors.forename: David A
authors.initials: DA
authors.lastname: Theilmann
authors.forename: Dwayne D
authors.initials: DD
authors.lastname: Hegedus
authors.forename: Douglas
authors.initials: D
authors.lastname: Baldwin
authors.forename: Martin A
authors.initials: MA
authors.lastname: Erlandson
mesh_terms: D000818:Animals; D041981:Gastrointestinal Tract / Q000821:virology; D059014:High-Throughput Nucleotide Sequencing; D007814:Larva / Q000821:virology; D007915:Lepidoptera / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome