title: Bone formation in adipose-derived stem cells isolated from elderly patients with osteoporosis: a preliminary study.
abstract: We have explored the osteogenic potency of adipose-derived stem cells from osteoporotic patients (opASCs). opASCs were osteogenically induced in vitro with collagen I hydrogel or in culture plate. Detection of alkaline phosphatase (ALPase) and cell mineralisation, and quantitative RT-PCR of collagen I, osteocalcin and bone sialoprotein were undertaken. Proliferation and morphology studies were also performed. After 14 days, opASCs-collagen I hydrogel composite was implanted into nude mice for 4 weeks prior to radiographic and histological analysis. Staining of ALPase and cell mineralisation was strongly positive in opASCs. Fibroblast-like opASCs induced with collagen I hydrogel were evenly distributed and proliferated at a higher rate than in culture plates, showing similar growth curves for both genders. Expression of ALPase activity, cell mineralisation and osteogenic specific genes were higher in opASCs with collagen I hydrogel (male samples had better osteogenicity than female samples) than in culture plates. After implantation for 4 weeks, radiopaque area signifying new bone tissue was observed in opASCs-collagen I hydrogel composite, with no donor gender differences. Thus opASCs with collagen I hydrogel have adequate osteogenic potency and offer new possibilities for osteoporosis-related bone tissue engineering in male and female patients.
authors.affiliation: Department of Stomatology, 107 Hospital of Ji'nan military area, Yantai, Shandong, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Jiang
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Liu
authors.forename: Lugang
authors.initials: L
authors.lastname: Zhou
authors.forename: Tingshu
authors.initials: T
authors.lastname: Jiang
authors.forename: Huihui
authors.initials: H
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Hao
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000368:Aged; D000818:Animals; D001842:Bone and Bones / Q000166:cytology* / Q000000981:diagnostic imaging / Q000473:pathology; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D058574:Integrin-Binding Sialoprotein / Q000235:genetics / Q000378:metabolism; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis; D010024:Osteoporosis / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D011859:Radiography
chemical_list: D058574:Integrin-Binding Sialoprotein; D015675:Osteocalcin; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
keywords: adipose-derived stem cells; bone formation; collagen I hydrogel; osteoporosis