title: Construction and expression of an antimicrobial peptide scolopin 1 from the centipede venoms of Scolopendra subspinipes mutilans in Escherichia coli using SUMO fusion partner.
abstract: Antimicrobial peptide scolopin 1 (AMP-scolopin 1) is a small cationic peptide identified from centipede venoms of Scolopendra subspinipes mutilans. It has broad-spectrum activities against bacteria, fungi, and tumor cells, which may possibly be used as an antimicrobial agent. We first report here the application of small ubiquitin-related modifier (SUMO) fusion technology to the expression and purification of cationic antimicrobial peptide AMP-scolopin 1. The fusion protein expressed in a soluble form was purified to a purity of 95% by Ni-IDA chromatography. After the SUMO-scolopin 1 fusion protein was cleaved by the SUMO protease at 30°C for 1 h, the cleaved sample was reapplied to a Ni-IDA. The recombinant scolopin1 had similar antimicrobial properties to the synthetic scolopin 1. Thus, we successfully established a system for purifying peptide of centipede, which could be used for further research.
authors.affiliation: Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Hou
authors.forename: Weili
authors.initials: W
authors.lastname: Yan
authors.forename: Kexing
authors.initials: K
authors.lastname: Du
authors.forename: Yangjing
authors.initials: Y
authors.lastname: Ye
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Cao
authors.forename: Wenhua
authors.initials: W
authors.lastname: Ren
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D001180:Arthropod Venoms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001181:Arthropods / Q000201:enzymology* / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D025841:Small Ubiquitin-Related Modifier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D001180:Arthropod Venoms; D011993:Recombinant Fusion Proteins; D025841:Small Ubiquitin-Related Modifier Proteins
keywords: AMP-scolopin 1; Antimicrobial peptide; Centipede; SUMO; Scolopendra subspinipes mutilans