title: Evaluation of chemical constituents and antioxidant activity of coconut water (Cocus nucifera L.) and caffeic acid in cell culture.
abstract: Coconut water contains several uncharacterized substances and is widely used in the human consumption. In this paper we detected and quantified ascorbic acid and caffeic acid and total phenolics in several varieties of coconut using HPLS/MS/MS (25.8 ± 0.6 µg/mL and 1.078 ± 0.013 µg/mL and 99.7 µg/mL, respectively, in the green dwarf coconut water, or 10 mg and 539 µg and 39.8 mg for units of coconut consumed, 500 ± 50 mL). The antioxidant potential of four coconut varieties (green dwarf, yellow dwarf, red dwarf and yellow Malaysian) was compared with two industrialized coconut waters and the lyophilized water of the green dwarf variety. All varieties were effective in scavenging the DPPH radical (IC₅₀=73 µL) and oxide nitric (0.1 mL with an IP of 29.9%) as well as in inhibiting the in vitro production of thiobarbituric acid reactive substances (1 mL with an IP of 34.4%), highlighting the antioxidant properties of the green dwarf which it is the most common used. In cell culture, the green dwarf water was efficient in protecting against oxidative damages induced by hydrogen peroxide.
authors.affiliation: Universidade Federal de Sergipe, Departamento de Fisiologia, São CristóvãoSE, Brasil.
authors.forename: João L A
authors.initials: JL
authors.lastname: Santos
authors.affiliation: Universidade Federal de Sergipe, Departamento de Fisiologia, São CristóvãoSE, Brasil.
authors.forename: Vanderson S
authors.initials: VS
authors.lastname: Bispo
authors.affiliation: Universidade Federal de Sergipe, Departamento de Fisiologia, São CristóvãoSE, Brasil.
authors.forename: Adriano B C
authors.initials: AB
authors.lastname: Filho
authors.affiliation: Universidade Federal de Sergipe, Departamento de Fisiologia, São CristóvãoSE, Brasil.
authors.forename: Isabella F D
authors.initials: IF
authors.lastname: Pinto
authors.affiliation: Universidade Federal de Sergipe, Departamento de Fisiologia, São CristóvãoSE, Brasil.
authors.forename: Lucas S
authors.initials: LS
authors.lastname: Dantas
authors.affiliation: Universidade Federal de Sergipe, Departamento de Fisiologia, São CristóvãoSE, Brasil.
authors.forename: Daiane F
authors.initials: DF
authors.lastname: Vasconcelos
authors.affiliation: Universidade Federal de Sergipe, Departamento de Fisiologia, São CristóvãoSE, Brasil.
authors.forename: Fabíula F
authors.initials: FF
authors.lastname: Abreu
authors.affiliation: Universidade Federal de Sergipe, Departamento de Fisiologia, São CristóvãoSE, Brasil.
authors.forename: Danilo A
authors.initials: DA
authors.lastname: Melo
authors.affiliation: Universidade Federal de Sergipe, Departamento de Fisiologia, São CristóvãoSE, Brasil.
authors.forename: Isaac A
authors.initials: IA
authors.lastname: Matos
authors.affiliation: Universidade de São Paulo, Instituto de Química, Departamento de Bioquímica, São PauloSP, Brasil.
authors.forename: Florêncio P
authors.initials: FP
authors.lastname: Freitas
authors.affiliation: Universidade de São Paulo, Instituto de Química, Departamento de Bioquímica, São PauloSP, Brasil.
authors.forename: Osmar F
authors.initials: OF
authors.lastname: Gomes
authors.affiliation: Universidade de São Paulo, Instituto de Química, Departamento de Bioquímica, São PauloSP, Brasil.
authors.forename: Marisa H G
authors.initials: MH
authors.lastname: Medeiros
authors.affiliation: Universidade Federal de Sergipe, Departamento de Fisiologia, São CristóvãoSE, Brasil.
authors.forename: Humberto R
authors.initials: HR
authors.lastname: Matos
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D001205:Ascorbic Acid / Q000032:analysis* / Q000494:pharmacology; D002109:Caffeic Acids / Q000032:analysis* / Q000494:pharmacology; D002478:Cells, Cultured; D003059:Cocos / Q000737:chemistry*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000166:cytology; D010636:Phenols / Q000032:analysis* / Q000494:pharmacology; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D002109:Caffeic Acids; D010636:Phenols; D001205:Ascorbic Acid; C040048:caffeic acid