title: [Effect of Notch1 signaling pathway activation on pancreatic cancer cell proliferation in vitro].
abstract: OBJECTIVE
  To observe the effect of activation of Notch1 signaling pathway by Notch intracellular domain (NICD) plasmid transfection on pancreatic cancer cell proliferation and explore the underlying mechanism.
  
  
  METHODS
  The transfection rates were observed under microscope with fluorescence stimulation, and mRNA expression levels of Hes1 were detected by real-time PCR. Cell proliferation changes were evaluated by CCK-8 after NICD and control plasmid transfection in pancreatic cancer cells. Caspase 3 activity was examined using a caspase 3 detection kit.
  
  
  RESULTS
  The transfection rates of NICD plasmid were up to 80% by fluorescence stimulation observation. Hes1 expression was significantly increased after NICD plasmid transfection, suggesting the activation of Notch1 signaling pathway. NICD plasmid transfection significantly promoted cancer cell proliferation compared to control plasmid transfeciton. The activities of caspase 3 were obviously decreased after NICD plasmid transfection in 3 pancreatic cancer cell lines.
  
  
  CONCLUSIONS
  Activation of Notch1 signaling pathway by NICD plasmid transfection can promote the proliferation of pancreatic cancer cells by inhibiting the apoptosis pathway.
authors.affiliation: Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu 610041, China. E-mail: duxiao_home@163.com.
authors.forename: Xiao
authors.initials: X
authors.lastname: Du
authors.forename: Siqin
authors.initials: S
authors.lastname: Zhang
authors.forename: Zhong
authors.initials: Z
authors.lastname: Cheng
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Chen
authors.forename: Jiankun
authors.initials: J
authors.lastname: Hu
authors.forename: Zongguang
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D010957:Plasmids; D051881:Receptor, Notch1 / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D000072056:Transcription Factor HES-1; D014162:Transfection
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D053148:Caspase 3; D018398:Homeodomain Proteins; D051881:Receptor, Notch1; D000072056:Transcription Factor HES-1; C497761:NOTCH1 protein, human; C078493:HES1 protein, human; C505407:CASP3 protein, human