title: Combination of 2DE and LC for plant proteomics analysis.
abstract: The use of analytical biochemical techniques with different separation properties allows us to better understand the proteome. To demonstrate this we have used two different methodologies to analyze embryos from a Tunisian cultivar of durum wheat (Triticum durum Desf.), variety Oum Rabiaa. We compared conventional 2D electrophoresis with liquid-phase chromatography. Our results show that a similar number of proteins were detected with both techniques. However, analysis of protein resolution at different pH ranges showed significant differences. By using a large pH gradient we observed that liquid chromatography presents higher resolution at extreme pH, either acidic or basic. Conversely, 2DE is more resolutive at intermediate pH (pH 5-6.5). Taking these results in consideration, we propose that 2DE and liquid chromatography are complementary methods to analyze complex protein extracts and can be used in parallel to acquire a wider perspective and a better understanding of the embryo proteome.
authors.affiliation: Centre de Recerca en Agrigenomica (CRAG), Campus UAB, Edificio CRAG, Bellaterra, Cerdanyola del Vallès, Barcelona, Spain.
authors.forename: Sami
authors.initials: S
authors.lastname: Irar
authors.forename: Faiçal
authors.initials: F
authors.lastname: Brini
authors.forename: Khaled
authors.initials: K
authors.lastname: Masmoudi
authors.forename: Montserrat
authors.initials: M
authors.lastname: Pagès
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D014908:Triticum / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D020543:Proteome