title: Laser-induced choroidal neovascularization model to study age-related macular degeneration in mice.
abstract: The mouse model of laser-induced choroidal neovascularization (CNV) has been used extensively in studies of the exudative form of age-related macular degeneration (AMD). This experimental in vivo model relies on laser injury to perforate Bruch's membrane, resulting in subretinal blood vessel recruitment from the choroid. By recapitulating the main features of the exudative form of human AMD, this assay has served as the backbone for testing antiangiogenic therapies. This standardized protocol can be applied to transgenic mice and can include treatments with drugs, recombinant proteins, antibodies, adenoviruses and pre-microRNAs to aid in the search for new molecular regulators and the identification of novel targets for innovative treatments. This robust assay requires 7-14 d to complete, depending on the treatment applied and whether immunostaining is performed. This protocol includes details of how to induce CNV, including laser induction, lesion excision, processing and different approaches to quantify neoformed vasculature.
authors.affiliation: 1] Department of Ophthalmology, University Hospital (CHU), Liège, Belgium. [2] Laboratory of Tumor and Development Biology, GIGA-Cancer, University of Liège, Liège, Belgium.
authors.forename: Vincent
authors.initials: V
authors.lastname: Lambert
authors.forename: Julie
authors.initials: J
authors.lastname: Lecomte
authors.forename: Sylvain
authors.initials: S
authors.lastname: Hansen
authors.forename: Silvia
authors.initials: S
authors.lastname: Blacher
authors.forename: Maria-Luz Alvarez
authors.initials: ML
authors.lastname: Gonzalez
authors.forename: Ingrid
authors.initials: I
authors.lastname: Struman
authors.forename: Nor Eddine
authors.initials: NE
authors.lastname: Sounni
authors.forename: Eric
authors.initials: E
authors.lastname: Rozet
authors.forename: Pascal
authors.initials: P
authors.lastname: de Tullio
authors.forename: Jean Michel
authors.initials: JM
authors.lastname: Foidart
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Rakic
authors.forename: Agnès
authors.initials: A
authors.lastname: Noel
mesh_terms: D000367:Age Factors; D000818:Animals; D002829:Choroid / Q000473:pathology*; D004195:Disease Models, Animal; D007834:Lasers; D008268:Macular Degeneration / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009389:Neovascularization, Pathologic