title: [Comparison of chemical components between Baishao and Chishao water extracts and their effects on proliferation of rat thoracic aorta smooth muscle cells].
abstract: OBJECTIVE
  To compare the chemical components in Baishao and Chishao water extracts and investigate their effects on the proliferation of rat thoracic aorta smooth muscle cells in vitro.
  
  
  METHODS
  The contents and chemical structures of monomers separated from the water extracts of Baishao and Chishao were analyzed using high-performance liquid chromatography and mass spectroscopy. Rat thoracic aorta smooth muscle cell line A7r5 and its platelet-derived growth factor-BB (PDGF-BB)-induced proliferation model were exposed to different concentrations of Baishao and Chishao water extracts, and the cell viability was analyzed by mitochondrial-dependent reduction of MTT and real-time cell analyzer.
  
  
  RESULTS
  The growth of A7r5 cells was significantly stimulated by 300 µg/ml Baishao water extract (P<0.01), but Chishao water extract produced no such effect (P>0.05). In PDGF-BB-induced cell proliferation model, the cell growth was significantly suppressed by 100-500 µg/ml Chishao water extract (P<0.01), while Baishao water extract showed no obvious effect on the cell proliferation (P>0.05).
  
  
  CONCLUSIONS
  Baishao and Chishao water extracts have different chemical components and produce different biological effects.
authors.affiliation: Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, China. E-mail: 664366571@qq.com.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Zhu
authors.forename: Yeuklung
authors.initials: Y
authors.lastname: Chow
authors.forename: Jinxiong
authors.initials: J
authors.lastname: Wu
authors.forename: Xian
authors.initials: X
authors.lastname: Zhang
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology*; D000077214:Becaplermin; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000187:drug effects; D029596:Paeonia / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology; D051381:Rats
chemical_list: D004365:Drugs, Chinese Herbal; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin