title: The complete mitochondrial genome sequence of Sogatella furcifera (Horváth) and a comparative mitogenomic analysis of three predominant rice planthoppers.
abstract: The white-backed planthopper (WBPH), Sogatella furcifera (Horváth), is one of the most destructive pests of rice crops in many Asian countries. Using long-PCR and shotgun library methods, we sequenced the entire mitochondrial genomes (mt-genomes) of two WBPH individuals. Total lengths of the mt-genome of the two WBPH individuals were 16,612 bp and 16,654 bp with an identical AT content of 76.19%. Among the 13 protein coding genes (PCGs), only nad5 used an atypical initiation codon GTG. Most of the tRNA genes had the typical cloverleaf secondary structure except that the dihydrouridine (DHU) arms in two trnS genes and the TΨC arm of trnG gene did not form a stable stem-loop structure. Similar to the brown planthopper (BPH), Nilaparvata lugens (Stål), and the small brown planthopper (SBPH), Laodelphax striatellus (Fallén), some extraordinary features were observed in the WBPH mt-genome. These include similar gene rearrangement pattern, unusually short length of the atp8 gene and variable numbers of tandem repeat (VNTR) structure in control region. Interestingly, the same tandem repeat unit with stable secondary structure appeared in two different planthoppers, WBPH and SBPH, which belong to two different genera of the Delphacidae. This peculiar feature provides a direct evidence for the close relationship between the two planthoppers and updates our understanding of the evolutionary characteristics of mitochondrial control region. Comparison with two other predominant rice planthoppers (BPH and SBPH) revealed that different PCGs of mitochondria exhibit different evolutionary patterns.
authors.affiliation: Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China; College of Plant Protection, Southwest University, Chongqing 400715, China.
authors.forename: Kai-Jun
authors.initials: KJ
authors.lastname: Zhang
authors.forename: Wen-Chao
authors.initials: WC
authors.lastname: Zhu
authors.forename: Xia
authors.initials: X
authors.lastname: Rong
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Xiu-Lei
authors.initials: XL
authors.lastname: Ding
authors.forename: Xiao-Yue
authors.initials: XY
authors.lastname: Hong
mesh_terms: D000818:Animals; D001483:Base Sequence; D018387:Codon, Initiator; D017931:DNA Primers; D054629:Genome, Mitochondrial; D006430:Hemiptera / Q000235:genetics*; D012275:Oryza; D016133:Polymerase Chain Reaction; D012343:RNA, Transfer / Q000235:genetics
chemical_list: D018387:Codon, Initiator; D017931:DNA Primers; D012343:RNA, Transfer
keywords: BPH; Comparative mitogenomic analysis; DHU; Gene rearrangement; ITS; Ile; Leu; Met; Mitochondrial control region; Mitochondrial genome; NADH dehydrogenase subunit 4; PCGs; Phe; RSCU; SBPH; Ser; Sogatella furcifera; VNTRs; Variable number of tandem repeats (VNTRs); WBPH; cox 1; cox 2; cox 3; cytochrome c oxidase subunit I; cytochrome c oxidase subunit III; cytochrome oxidase subunit II; dihydrouridine; isoleucine; leucine; methionine; mitochondrial genome; mt-genome; nad4; phenylalanine; protein coding genes; serine; tRNA; the brown planthopper, Nilaparvata lugens (Stål); the internal transcribed spacer; the relative synonymous codon usage; the small brown planthopper, Laodelphax striatellus (Fallén); the white-backed planthopper, Sogatella furcifera (Horváth); transfer RNA; variable numbers of tandem repeats