title: Peripheral blood progenitor cell collection by two programs for autologous and allogeneic transplantation.
abstract: BACKGROUND
  In the Spectra apheresis instrument (Terumo BCT), both manual (Spectra-MNC) and automated (Spectra-Auto) programs have been widely used to collect peripheral blood progenitor cells (PBPCs). However, direct comparison of these programs remains extremely limited.
  
  
  METHODS
  We investigated 188 collections and products from autologous (patient) and allogeneic (donor) subjects and analyzed a subset of 89 allogeneic collections and products. Twenty-nine subjects who received apheresis for 2 consecutive days using both programs were also evaluated with a paired crossover comparison.
  
  
  RESULTS
  The two programs processed similar volumes, but run time was longer with Spectra-Auto. Yield and efficiency of CD34+ cell collection were similar between these programs in the whole cohort, although white blood cell (WBC) and mononuclear cell (MNC) yields were higher with Spectra-MNC. In the allogeneic cohort, yield and efficiency of WBC collection were greater in Spectra-MNC. However, collected WBCs, MNCs, and CD34+ cells were similar between these programs in paired comparison. Regardless of program, preapheresis peripheral WBC, MNC, and CD34+ cell counts correlated with the number of cells collected. In contrast, preapheresis WBC counts in the whole cohort were negatively correlated with collection efficiencies of CD34+ cells in Spectra-MNC but not Spectra-Auto. The products collected using Spectra-MNC contained more contaminating platelets (PLTs) than Spectra-Auto, with a corresponding reduction in postdonation circulating PLTs.
  
  
  CONCLUSIONS
  Spectra-MNC and Spectra-Auto showed distinct features that should be considered on a case-by-case basis. Similar investigations should be undertaken as new collection platforms are introduced.
authors.affiliation: Department of Blood Transfusion and Transplantation Immunology, Fukushima Medical University, Fukushima, Japan; Department of Cardiology and Hematology, Fukushima Medical University, Fukushima, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ikeda
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ohto
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kanno
authors.forename: Kenji
authors.initials: K
authors.lastname: Gonda
authors.forename: Yuhko
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Takako
authors.initials: T
authors.lastname: Ono
authors.forename: Shunnichi
authors.initials: S
authors.lastname: Saito
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takahashi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kimura
authors.forename: Kayo
authors.initials: K
authors.lastname: Harada-Shirado
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamauchi
authors.forename: Yasuto
authors.initials: Y
authors.lastname: Hoshino
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Mashimo
authors.forename: Kenneth E
authors.initials: KE
authors.lastname: Nollet
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kikuta
authors.forename: Kazuei
authors.initials: K
authors.lastname: Ogawa
authors.forename: Yasuchika
authors.initials: Y
authors.lastname: Takeishi
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000097:blood; D001781:Blood Component Removal / Q000379:methods*; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation; D013921:Thrombocytopenia / Q000209:etiology; D014182:Transplantation, Autologous
chemical_list: D018952:Antigens, CD34