title: Triggered sorting and co-assembly of genetically engineered protein microdomains in the cytoplasm.
abstract: Elastin-like polypeptides (ELPs) are genetically encoded protein polymers that reversibly phase separate in response to stimuli. They respond sharply to small shifts in temperature and form dense microdomains in the living eukaryotic cytosol. For the first time, this communication illustrates how to tune the ELP sequence and architecture for either co-assembly or sorting of distinct proteins into microdomains within a living cell.
authors.affiliation: Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA, 90089, USA.
authors.forename: Pu
authors.initials: P
authors.lastname: Shi
authors.forename: Yi-An
authors.initials: YA
authors.lastname: Lin
authors.forename: Martha
authors.initials: M
authors.lastname: Pastuszka
authors.forename: Honggang
authors.initials: H
authors.lastname: Cui
authors.forename: J Andrew
authors.initials: JA
authors.lastname: Mackay
mesh_terms: D002460:Cell Line; D003600:Cytosol / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics; D006801:Humans; D016014:Linear Models; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics; D018613:Microscopy, Confocal; D008970:Molecular Weight; D053758:Nanoparticles / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics; D015202:Protein Engineering; D017434:Protein Structure, Tertiary; D013696:Temperature; D014162:Transfection; D044366:Transition Temperature
chemical_list: D008164:Luminescent Proteins; D010455:Peptides; C413663:fluorescent protein 583; D049452:Green Fluorescent Proteins
keywords: confocal microscopy; elastin-like polypeptides; intracellular sorting; macromolecular assembly; protein polymer