title: Case-control study of genus-beta human papillomaviruses in plucked eyebrow hairs and cutaneous squamous cell carcinoma.
abstract: Cutaneous human papillomaviruses (HPV) have been reported in cutaneous squamous cell carcinoma (SCC). We conducted a clinic-based case-control study to investigate the association between genus-beta HPV DNA in eyebrow hairs (EBH) and SCC. EBH from 168 SCC cases and 290 controls were genotyped for genus-beta HPV DNA. SCC tumors from a subset of cases (n = 142) were also genotyped. Viral load was determined in a subset of specimens positive for a single HPV type. Associations with SCC were estimated by odds ratios (OR) and 95% confidence intervals (CI) adjusted for age and sex using logistic regression. Statistical tests were two-sided. EBH DNA prevalence was greater in cases (87%) than controls (73%) (p < 0.05), and the association with SCC increased with the number of HPV types present, (≥ 4 types vs. HPV-negative: OR = 2.02, 95% CI = 1.07-3.80; p(trend) = 0.02). Type-specific associations were observed between SCC and DNA in EBH for HPV23 (OR = 1.90, 95% CI = 1.10-3.30) and HPV38 (OR = 1.84, 95% CI = 1.04-3.24). Additionally, when compared with the controls, the DNA prevalence in EBH was significantly higher among cases for 11 of the 25 genus-beta types tested, when accounting for DNA for the same HPV type in the tumor (ORs = 3.44-76.50). Compared to controls, the mean viral DNA load in EBH among the selected cases was greater for HPV5, HPV8 and HPV24, but lower for HPV38. SCC cases were more likely than controls to have HPV DNA+ EBH for single and multiple HPV types, providing additional support for the potential role of genus-beta HPV infections in SCC development.
authors.affiliation: Infections and Cancer Biology (ICB) Group, International Agency for Research on Cancer, Lyon, Cedex, 08, France.
authors.forename: Michelle R
authors.initials: MR
authors.lastname: Iannacone
authors.forename: Tarik
authors.initials: T
authors.lastname: Gheit
authors.forename: Herbert
authors.initials: H
authors.lastname: Pfister
authors.forename: Anna R
authors.initials: AR
authors.lastname: Giuliano
authors.forename: Jane L
authors.initials: JL
authors.lastname: Messina
authors.forename: Neil A
authors.initials: NA
authors.lastname: Fenske
authors.forename: Basil S
authors.initials: BS
authors.lastname: Cherpelis
authors.forename: Vernon K
authors.initials: VK
authors.lastname: Sondak
authors.forename: Richard G
authors.initials: RG
authors.lastname: Roetzheim
authors.forename: Steffi
authors.initials: S
authors.lastname: Silling
authors.forename: Michael
authors.initials: M
authors.lastname: Pawlita
authors.forename: Massimo
authors.initials: M
authors.lastname: Tommasino
authors.forename: Dana E
authors.initials: DE
authors.lastname: Rollison
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D052686:Betapapillomavirus / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000821:virology*; D016022:Case-Control Studies; D004279:DNA, Viral / Q000032:analysis; D005138:Eyebrows / Q000821:virology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000821:virology*; D019562:Viral Load; D055815:Young Adult
chemical_list: D004279:DNA, Viral
keywords: DNA; case-control; eyebrow hairs; genus-beta human papillomaviruses; squamous cell carcinoma