title: Ca analysis: an Excel based program for the analysis of intracellular calcium transients including multiple, simultaneous regression analysis.
abstract: Here I present an Excel based program for the analysis of intracellular Ca transients recorded using fluorescent indicators. The program can perform all the necessary steps which convert recorded raw voltage changes into meaningful physiological information. The program performs two fundamental processes. (1) It can prepare the raw signal by several methods. (2) It can then be used to analyze the prepared data to provide information such as absolute intracellular Ca levels. Also, the rates of change of Ca can be measured using multiple, simultaneous regression analysis. I demonstrate that this program performs equally well as commercially available software, but has numerous advantages, namely creating a simplified, self-contained analysis workflow.
authors.affiliation: Unit of Cardiac Physiology, Institute of Cardiovascular Science, Manchester Academic Health Science Centre, Core Technology Facility, 46 Grafton Street, M13 9NT, UK. Electronic address: david.greensmith@manchester.ac.uk.
authors.forename: David J
authors.initials: DJ
authors.lastname: Greensmith
mesh_terms: D002118:Calcium / Q000378:metabolism*; D012044:Regression Analysis; D012984:Software
chemical_list: D002118:Calcium
keywords: Calcium transient; Excel; Fluorescent indicators; Intracellular calcium; Regression analysis; Visual basic for applications