title: Effects of vegetable oils on biochemical and biophysical properties of membrane retinal pigment epithelium cells.
abstract: The aim of this study was to investigate the effect of vegetable oil enrichment of retinal pigment epithelial (RPE) cells on their biochemical and biophysical properties. For this, RPE cells were incubated with 4 different vegetables oils (olive oil, corn oil, argan oil, and camelina oil). The cytotoxicity of these vegetable oils was assessed in vivo on 8-week-old mice and in vitro by using the neutral red and YO-PRO-1 tests. Membrane fluidity was evaluated by fluorescence anisotropy using the fluorescent probe diphenylhexatriene, and membrane fatty acid composition was assessed by gas chromatography. None of the oils tested displayed cytotoxic effects. In vitro, omega-3 rich oils improved membrane fluidity by 47% compared with the control cells. The omega-3 PUFA content within membranes decreased by 38% to 55% when cells were incubated separately with olive oil, corn oil, or argan oil, and increased when cells were incubated with a mixture of those oils, or with camelina oil alone (50% and 103% increase, respectively). Our results show that the fatty acids in vegetable oil incorporate into retinal cells and increase the plasma membrane fluidity.
authors.affiliation: a Research Center on Aging, Fleurimont Site, 3001, 12ème Avenue Nord, Sherbrooke, QC J1H 4N4, Canada.
authors.forename: Toihiri
authors.initials: T
authors.lastname: Said
authors.forename: Jennifer
authors.initials: J
authors.lastname: Tremblay-Mercier
authors.forename: Hicham
authors.initials: H
authors.lastname: Berrougui
authors.forename: Patrice
authors.initials: P
authors.lastname: Rat
authors.forename: Abdelouahed
authors.initials: A
authors.lastname: Khalil
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D003314:Corn Oil / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D005260:Female; D006801:Humans; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D008563:Membrane Lipids / Q000378:metabolism; D051379:Mice; D000069463:Olive Oil; D010938:Plant Oils / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D055213:Retinal Pigment Epithelium / Q000187:drug effects* / Q000378:metabolism
chemical_list: D015525:Fatty Acids, Omega-3; D008563:Membrane Lipids; D000069463:Olive Oil; D010938:Plant Oils; C555574:argan oil; D003314:Corn Oil