title: Upregulation of membrane-bound CD40L on CD4+ T cells in women with primary Sjögren's syndrome.
abstract: Epigenetic deregulation of genes encoded on the X chromosome as reported for CD40L in lupus could explain the female predominance of autoimmune diseases. We compared CD40L expression on CD4(+) T cells from primary Sjögren's syndrome (pSS) women and healthy controls and investigated DNA methylation patterns of the promoter and enhancer regions of CD40L. The expression of CD40L on activated CD4(+) T cells was higher in patients with pSS than controls after phorbolmyristate acetate and ionomycin activation (P = 0.02). CD40L mRNA level in CD4(+) T cells did not differ between patients with pSS and controls and was similar in both groups in cultures treated with the demethylating agent 5-azacytidine C. Pyrosequencing analysis revealed no significant differences in methylation profiles between patients and controls. Inducible membrane-bound CD40L on CD4(+) T cells is increased in patients with pSS but was not related to epigenetic deregulation by demethylation patterns of the regulatory regions of CD40L.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM) U1012, Le Kremlin Bicêtre, France; Université Paris-Sud, Hôpitaux Universitaires Paris-Sud, AP-HP, Le Kremlin Bicêtre, France.
authors.forename: R
authors.initials: R
authors.lastname: Belkhir
authors.forename: N
authors.initials: N
authors.lastname: Gestermann
authors.forename: M
authors.initials: M
authors.lastname: Koutero
authors.forename: R
authors.initials: R
authors.lastname: Seror
authors.forename: J
authors.initials: J
authors.lastname: Tost
authors.forename: X
authors.initials: X
authors.lastname: Mariette
authors.forename: C
authors.initials: C
authors.lastname: Miceli-Richard
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001374:Azacitidine / Q000494:pharmacology; D023201:CD40 Ligand / Q000235:genetics / Q000276:immunology*; D002478:Cells, Cultured; D019175:DNA Methylation / Q000187:drug effects / Q000276:immunology; D005260:Female; D015870:Gene Expression / Q000276:immunology; D006801:Humans; D015759:Ionomycin / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D008875:Middle Aged; D011401:Promoter Regions, Genetic / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods; D012859:Sjogren's Syndrome / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives / Q000494:pharmacology; D015854:Up-Regulation / Q000276:immunology*; D055815:Young Adult
chemical_list: D008565:Membrane Proteins; D023201:CD40 Ligand; D015759:Ionomycin; C016658:phorbolol myristate acetate; D001374:Azacitidine; D013755:Tetradecanoylphorbol Acetate