title: Photochemical modulation of biosafe manganese nanoparticles on Vigna radiata: a detailed molecular, biochemical, and biophysical study.
abstract: Manganese (Mn) is an essential element for plants which intervenes mainly in photosynthesis. In this study we establish that manganese nanoparticles (MnNP) work as a better micronutrient than commercially available manganese salt, MnSO4 (MS) at recommended doses on leguminous plant mung bean (Vigna radiata) under laboratory condition. At higher doses it does not impart toxicity to the plant unlike MS. MnNP-treated chloroplasts show greater photophosphorylation, oxygen evolution with respect to control and MS-treated chloroplasts as determined by biophysical and biochemical techniques. Water splitting by an oxygen evolving complex is enhanced by MnNP in isolated chloroplast as confirmed by polarographic and spectroscopic techniques. Enhanced activity of the CP43 protein of a photosystem II (PS II) Mn4Ca complex influenced better phosphorylation in the electron transport chain in the case of MnNP-treated chloroplast, which is evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and corresponding Western blot analysis. To the best of our knowledge this is the first report to augment photosynthesis using MnNP and its detailed correlation with different molecular, biochemical and biophysical parameters of photosynthetic pathways. At effective dosage, MnNP is found to be biosafe both in plant and animal model systems. Therefore MnNP would be a novel potential nanomodulator of photochemistry in the agricultural sector.
authors.affiliation: Biological Sciences Division, Indian Statistical Institute , 203 B.T. Road, Kolkata 700108, India.
authors.forename: Saheli
authors.initials: S
authors.lastname: Pradhan
authors.forename: Prasun
authors.initials: P
authors.lastname: Patra
authors.forename: Sumistha
authors.initials: S
authors.lastname: Das
authors.forename: Sourov
authors.initials: S
authors.lastname: Chandra
authors.forename: Shouvik
authors.initials: S
authors.lastname: Mitra
authors.forename: Kushal Kumar
authors.initials: KK
authors.lastname: Dey
authors.forename: Shirin
authors.initials: S
authors.lastname: Akbar
authors.forename: Pratip
authors.initials: P
authors.lastname: Palit
authors.forename: Arunava
authors.initials: A
authors.lastname: Goswami
mesh_terms: D018533:Biomass; D002734:Chlorophyll / Q000378:metabolism; D004579:Electron Transport / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D008345:Manganese / Q000494:pharmacology*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D018384:Oxidative Stress / Q000187:drug effects; D010777:Photochemistry; D010788:Photosynthesis / Q000187:drug effects; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D018520:Plant Shoots / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D010940:Plant Proteins; D002734:Chlorophyll; D008345:Manganese