title: An innovative sample-to-answer polymer lab-on-a-chip with on-chip reservoirs for the POCT of thyroid stimulating hormone (TSH).
abstract: A new sample-to-answer polymer lab-on-a-chip, which can perform immunoassay with minimum user intervention through on-chip reservoirs for reagents and single-channel assay system, has been designed, developed and successfully characterized as a point-of-care testing (POCT) cartridge for the detection of thyroid stimulating hormone (TSH). Test results were obtained within 30 minutes after a sample was dropped into the POCT cartridge. The analyzed results of TSH showed a linear range of up to 55 μIU mL(-1) with the limit of detection (LOD) of 1.9 μIU mL(-1) at the signal-to-noise ratio (SNR) of 3. The reagents stored in the on-chip reservoirs maintained more than 97% of their initial volume for 120 days of storage time while the detection antibody retained its activity above 98% for 120 days. The sample-to-answer polymer lab-on-a-chip developed in this work using the mass-producible and low-cost polymer is well suited for the point-of-care testing of rapid in vitro diagnostics (IVD) of TSH.
authors.affiliation: Microsystems and BioMEMS Laboratory, University of Cincinnati, Cincinnati, OH 45221, USA. jungwk@mail.uc.edu.
authors.forename: Wooseok
authors.initials: W
authors.lastname: Jung
authors.forename: Jungyoup
authors.initials: J
authors.lastname: Han
authors.forename: Junhai
authors.initials: J
authors.lastname: Kai
authors.forename: Ji-Youn
authors.initials: JY
authors.lastname: Lim
authors.forename: Donggeun
authors.initials: D
authors.lastname: Sul
authors.forename: Chong H
authors.initials: CH
authors.lastname: Ahn
mesh_terms: D000906:Antibodies / Q000276:immunology; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D019095:Point-of-Care Systems; D011108:Polymers / Q000737:chemistry*; D059629:Signal-To-Noise Ratio; D013972:Thyrotropin / Q000032:analysis* / Q000276:immunology
chemical_list: D000906:Antibodies; D011108:Polymers; D013972:Thyrotropin