title: [Relationship between single nucleotide polymorphisms and its haplotype of X-ray repair cross complementing group 1 and susceptibility of pancreatic carcinoma].
abstract: OBJECTIVE
  The aim of this study was to evaluate the effects of XRCCl gene polymorphisms and its haplotype on the susceptibility of pancreatic carcinoma.
  
  
  METHODS
  Peripheral blood DNA was extracted from 210 pancreatic carcinoma patients and 213 control subjects. SNaPshot technique was used for genotyping seven SNP sites of the XRCCl gene (rs3213403, rs25487, rs1799782, rs731420, rs1001581, rs12611088, and rs3213282). Logistic regression model was performed to analyze the relationship of different genotypes or haplotype and the susceptibility of pancreatic carcinoma.
  
  
  RESULTS
  The frequency for allele A at site rs25487 in the case group was significantly higher than that in the control group (P < 0.05). The frequency of GG, GA and AA genotype between the case group and control group had statistically significant differences (P < 0.05). Compared with GG genotype, the risk of pancreatic carcinoma in the subjects carrying mutated allele A (GA+AA) was increased by 0.648 times (P < 0.05). Among them the pancreatic carcinoma risk of individuals carrying A allele was increased by 0.552 times compared with the individuals carrying G allele. The frequency of allele and genotype at site rs1799782 in the case group and control group had a significant difference (P < 0.05). Compared with the CC genotype, the risk of pancreatic carcinoma in the subjects carrying mutated allele T (CT+TT) was increased by 0.683 times. Among them the pancreatic carcinoma risk of individuals carrying T allele was increased by 0.549 times compared with the individuals carrying C allele. Significant differences were observed in linkage disequilibrium between any two of the seven SNPs (P < 0.05), the frequency of H4-AGCCCGC, H6-GGCCCGG or H7-AGCCTAG haplotypes was significantly lower in the case group than that in the control group (P < 0.05).
  
  
  CONCLUSIONS
  The single nucleotide polymorphisms of rs25487 and rs1799782 for XRCC1 gene may be correlated with the occurrence of pancreatic carcinoma. The haplotypes of H4-AGCCCGC, H6-GGCCCGG and H7-AGCCTAG might be a potential genetic protective factor for the occurrence of pancreatic carcinoma.
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Tumor Hospital, Xinjiang Medical University, Urumqi 830011, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Yan
authors.forename: Xi-yan
authors.initials: XY
authors.lastname: Wang
authors.forename: Hai-jun
authors.initials: HJ
authors.lastname: Li
authors.forename: Xin-jian
authors.initials: XJ
authors.lastname: Xu
authors.forename: Gong-bing
authors.initials: GB
authors.lastname: Zhu
authors.forename: Tie-ying
authors.initials: TY
authors.lastname: He
mesh_terms: D000483:Alleles; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D020022:Genetic Predisposition to Disease / Q000453:epidemiology*; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D010190:Pancreatic Neoplasms / Q000453:epidemiology*; D020641:Polymorphism, Single Nucleotide; D014965:X-Rays; D000076105:X-ray Repair Cross Complementing Protein 1
chemical_list: D004268:DNA-Binding Proteins; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human