title: [Pain assessment of biological conduit small gap tubulization in rat sciatic nerve multilation model].
abstract: OBJECTIVE
  To explore the pain sensation recovery discipline of 2 mm small gap biological conduit tubulization and epineurial neurorrhaphy in rat sciatic nerve multilation model.
  
  
  METHODS
  Based on the rat sciatic nerve multilation model, 2 mm small gap biological conduit tubulization and epineurial neurorrhaphy were applied and the 50% paw withdrawal threshold was observed after 2, 4, 5, 6, 8 and 12 weeks. The data were analyzed by two-way ANOVA and chi-square criterion.
  
  
  RESULTS
  Obvious hyperalgesia was observed in week 2 in both experimental group and control group, and 50% paw withdrawal threshold was improved significantly even to 15 g. The 50% paw withdrawal threshold began to decline week 4 and the 50% paw withdrawal threshold of small gap tubulization group was obviously lower than that of control group, which may imply that the pain sensation recovery of small gap tubulization group was earlier than that of control group. The 50% paw withdrawal threshold of small gap tubulization group began to increase to the plateau period [week 5: (12.70 ± 5.64) g; week 6: (12.20 ± 3.26) g; week 8: (12.31 ± 4.19) g; week 12: (13.95 ± 2.58) g]. The 50% paw withdrawal threshold of control group declined gradually [week 5: (10.47 ± 7.02) g; week 6: (9.42 ± 6.86) g; week 8: (8.50 ± 7.15) g; week 12: (8.06 ± 5.93) g]. The difference was statistical significant between small gap tubulization group and control group in 12th week.
  
  
  CONCLUSIONS
  Compared with the traditional epineurial neurorrhaphy for peripheral nerve multilation, 2 mm small gap biological conduit tubulization can improve the 50% paw withdrawal threshold during peripheral nerve regeneration process and reduce the pain incidence.
authors.affiliation: Department of Trauma and Orthopeadics, Peking University People's Hospital; Peking University Traffic Medicine Center, Beijing 100044, China.
authors.forename: Pei-Xun
authors.initials: PX
authors.lastname: Zhang
authors.forename: Shuai
authors.initials: S
authors.lastname: An
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Wang
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.forename: Zhen-Wei
authors.initials: ZW
authors.lastname: Wang
authors.forename: Na
authors.initials: N
authors.lastname: Han
authors.forename: Yu-Hui
authors.initials: YH
authors.lastname: Kou
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Bao-Guo
authors.initials: BG
authors.lastname: Jiang
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials; D005260:Female; D006930:Hyperalgesia / Q000503:physiopathology / Q000628:therapy; D008297:Male; D009416:Nerve Regeneration; D019635:Neurosurgical Procedures; D010147:Pain Measurement; D017288:Pain Threshold; D059348:Peripheral Nerve Injuries / Q000503:physiopathology / Q000628:therapy*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries* / Q000503:physiopathology; D013536:Suture Techniques
chemical_list: D001672:Biocompatible Materials