title: [Inhibitory effect of luteolin on the angiogenesis of chick chorioallantoic membrane and invasion of breast cancer cells via downregulation of AEG-1 and MMP-2].
abstract: The purpose of the present study was to investigate the effect of luteolin on the angiogenesis and invasion of breast cancer cells. MTT assay was used to examine breast cancer proliferation. The chick chorioallantoic membrane model was used to assess the angiogenesis effect. Wound healing assay was used to assess cell invasion ability. Western blot was used to analyze Bcl-2, AEG-1 and MMP-2 expression levels. The results showed luteolin inhibited MCF-7 cells proliferation in a dose- and time-dependent manner, and the expression of Bcl-2 protein was decreased. Luteolin had a strong anti-angiogenesis of chick chorioallantoic membrane. After treatment of MCF-7 cells with luteolin at 60 μmol/L for 48 h, migration rate was reduced by 71.07% compared with control (P < 0.01). After treatment of MCF-7 cells with luteolin at 60 μmol/L for 48 h, the expression of AEG-1 and MMP-2 was reduced by 82.34% (P < 0.05) and 85.70% (P < 0.05) respectively, compared with control. In conclusion, the results suggest that luteolin can inhibit the proliferation of breast cancer cells, and suppress the expression of Bcl-2. Furthermore, luteolin has strong anti-angiogenesis of chick chorioallantoic membrane and anti-invasive activity on breast cancer cells, and down-regulates the expression of AEG-1 and MMP-2.
authors.affiliation: Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, College of Life Science, Liaoning Normal University, Dalian 116081, China. xmj1222@sina.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Jiang
authors.forename: Kun-Peng
authors.initials: KP
authors.lastname: Xie
authors.forename: Hong-Nan
authors.initials: HN
authors.lastname: Huo
authors.forename: Li-Meng
authors.initials: LM
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Zou
authors.forename: Ming-Jie
authors.initials: MJ
authors.lastname: Xie
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D049109:Cell Proliferation; D002645:Chickens; D049033:Chorioallantoic Membrane / Q000187:drug effects*; D015536:Down-Regulation; D005260:Female; D006801:Humans; D047311:Luteolin / Q000494:pharmacology*; D061986:MCF-7 Cells; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D008565:Membrane Proteins; D009389:Neovascularization, Pathologic / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D016601:RNA-Binding Proteins
chemical_list: D015815:Cell Adhesion Molecules; C483039:MTDH protein, human; D008565:Membrane Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016601:RNA-Binding Proteins; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D047311:Luteolin