title: Construction of novel cytokine by fusion of chicken IL-2 signal peptide to mature chicken IL-15 and comparison of the adjuvant effects by DNA immunization against Eimeria challenge.
abstract: A novel fusion cytokine was constructed by replacing signal peptide (SP) of chicken IL-15 (ChIL-15) with SP of chicken IL-2 (ChIL-2). The fusion cytokine (NChIL-15) was cloned into the expression vector pcDNA3.1(+) to generate pcDNA-NChIL-15. An animal experiment was carried out to evaluate the adjuvant effects of NChIL-15 on DNA vaccine pcDNA-3-1E against Eimeria acervulina challenge. The mRNA profiles of ChIL-2 and ChIFN-γ in spleen were characterized by means of real-time PCR. The recombinant positive eukaryotic expression plasmid pcDNA-NChIL-15 were constructed successfully. The protective effects provided by co-immunization with 100 μg pcDNA-3-1E and 50 μg pcDNA-NChIL-15, measured by relative body weight gain (BWG), average lesion score in duodenum and oocyst decrease ratio, showed no significant difference with 50 μg pcDNA-ChIL-15 as an adjuvant on day 6 post infection (PI). However, chickens co-immunized with pcDNA-3-1E and pcDNA-NChIL-15 exhibited significant upregulated level of ChIL-2 and ChIFN-γ transcripts in spleen. Our original data suggests the constructed novel cytokine NChIL-15 could be a potential adjuvant used to enhance the immune protective effects, although the optimized dosage need to be explored further.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China.
authors.forename: Dexing
authors.initials: D
authors.lastname: Ma
authors.forename: Mingyang
authors.initials: M
authors.lastname: Gao
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Chunli
authors.initials: C
authors.lastname: Ma
authors.forename: Guangxing
authors.initials: G
authors.lastname: Li
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D004539:Eimeria / Q000276:immunology*; D019409:Interleukin-15 / Q000235:genetics*; D007376:Interleukin-2 / Q000235:genetics*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics*; D016052:Protozoan Vaccines / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000494:pharmacology*; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D019409:Interleukin-15; D007376:Interleukin-2; D021382:Protein Sorting Signals; D016052:Protozoan Vaccines; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA
keywords: Adjuvant effects; DNA vaccine; Eimeria acervulina; Novel cytokine