title: MRT letter: light sheet based imaging flow cytometry on a microfluidic platform.
abstract: We propose a light sheet based imaging flow cytometry technique for simultaneous counting and imaging of cells on a microfluidic platform. Light sheet covers the entire microfluidic channel and thus omits the necessity of flow focusing and point scanning based technology. Another advantage lies in the orthogonal detection geometry that totally cuts-off the incident light, thereby substantially reducing the background in the detection. Compared to the existing state-of-art techniques the proposed technique shows marked improvement. Using fluorescently-coated Saccharomyces cerevisiae cells we have recorded cell counting with throughput as high as 2,090 cells/min in the low flow rate regime and were able to image the individual cells on-the-go. Overall, the proposed system is cost-effective and simple in channel geometry with the advantage of efficient counting in operational regime of low laminar flow. This technique may advance the emerging field of microfluidic based cytometry for applications in nanomedicine and point of care diagnostics.
authors.affiliation: Nanobioimaging Laboratory, Department of Instrumentation and Applied Physics, Indian Institute of Science, Bangalore, 560012, India.
authors.forename: Raju
authors.initials: R
authors.lastname: Regmi
authors.forename: Kavya
authors.initials: K
authors.lastname: Mohan
authors.forename: Partha P
authors.initials: PP
authors.lastname: Mondal
mesh_terms: D015169:Colony Count, Microbial / Q000379:methods; D003584:Cytological Techniques / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D044085:Microfluidics / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000166:cytology; D013194:Staining and Labeling / Q000379:methods
chemical_list: D005456:Fluorescent Dyes
keywords: cytometry; fluorescence; imaging; light-sheet microscopy