title: Involvement of BDNF and NGF in the mechanism of neuroprotective effect of human recombinant erythropoietin nanoforms.
abstract: Human recombinant erythropoietin adsorbed on poly(butyl)cyanoacrylate nanoparticles and administered intraperitoneally in a dose of 0.05 mg/kg exhibited a neuroprotective effect in experimental intracerebral posttraumatic hematomas (hemorrhagic stroke) and reduced animal mortality. Human recombinant erythropoietin, native and adsorbed on lactic and glycolic acid copolymer-based nanoparticles, exhibited no antistroke effect on this model. Analysis of reverse transcription PCR products showed that human recombinant erythropoietin adsorbed on poly(butyl)cyanoacrylate nanoparticles more than 2-fold increased the expression of BDNF and NGF neurotrophins in the rat brain frontal cortex and hippocampus.
authors.affiliation: Laboratory of Psychopharmacology, V. V. Zakusov Institute of Pharmacology, the Russian Academy of Medical Sciences, Moscow, Russia. bal.pharm@mail.ru.
authors.forename: I N
authors.initials: IN
authors.lastname: Solev
authors.forename: V Yu
authors.initials: VY
authors.lastname: Balabanyan
authors.forename: I A
authors.initials: IA
authors.lastname: Volchek
authors.forename: O S
authors.initials: OS
authors.lastname: Elizarova
authors.forename: S A
authors.initials: SA
authors.lastname: Litvinova
authors.forename: T L
authors.initials: TL
authors.lastname: Garibova
authors.forename: T A
authors.initials: TA
authors.lastname: Voronina
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis / Q000378:metabolism*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D002543:Cerebral Hemorrhage / Q000188:drug therapy / Q000401:mortality; D003487:Cyanoacrylates / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D004659:Enbucrilate; D004921:Erythropoietin / Q000378:metabolism*; D006406:Hematoma / Q000188:drug therapy / Q000401:mortality; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008297:Male; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism / Q000627:therapeutic use*; D020932:Nerve Growth Factor / Q000096:biosynthesis / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000378:metabolism / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000378:metabolism / Q000627:therapeutic use; D020521:Stroke / Q000188:drug therapy* / Q000401:mortality
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D003487:Cyanoacrylates; D018696:Neuroprotective Agents; D011994:Recombinant Proteins; D004921:Erythropoietin; D020932:Nerve Growth Factor; D004659:Enbucrilate