title: Effect of rhynchophylline on the expression of p-CREB and sc-Fos in triatum and hippocampal CA1 area of methamphetamine-induced conditioned place preference rats.
abstract: To explore the effect of rhynchophylline (Rhy) on the expression of p-CREB and c-Fos in the striatum and hippocampal CA1 area of methamphetamine-induced conditioned place preference (CPP) rat, methamphetamine (2 mg/kg) was injected to rats and the conditioned place preference was observed in these rats treated with or without Rhy. An immunohistochemistry assay was used to determine the expression of p-CREB and c-Fos in the striatum and hippocampal CA1 area. Methamphetamine induced significant behavior alteration in CPP, while after pretreatment with rhynchophylline or ketamine, the time of staying in methamphetamine-paired compartment of rats was significantly reduced. Methamphetamine also increased the number of p-CREB positive cells in the striatum and hippocampal CA1 zone, as well as p-Fos positive cells. However, the compound Rhy could attenuate the effect. These findings show that Rhy can suppress the acquisition of CPP in rats induced by methamphetamine and the action may be related with the reduced expression of p-CREB and p-Fos in the striatum and hippocampus.
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong.
authors.forename: Qiu-Xian
authors.initials: QX
authors.lastname: Peng
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Xiao-Liang
authors.initials: XL
authors.lastname: Lin
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Chao-Hua
authors.initials: CH
authors.lastname: Luo
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Ming-Jin
authors.initials: MJ
authors.lastname: Jiang
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China. Electronic address: cherrymo@fimmu.com.
authors.forename: Zhi-Xian
authors.initials: ZX
authors.lastname: Mo
authors.affiliation: Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong. Electronic address: kklyung@hkbu.edu.hk.
authors.forename: Ken Kin-Lam
authors.initials: KK
authors.lastname: Yung
mesh_terms: D019969:Amphetamine-Related Disorders / Q000378:metabolism* / Q000517:prevention & control; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D050882:CREB-Binding Protein / Q000378:metabolism*; D003216:Conditioning, Operant / Q000187:drug effects*; D006624:Hippocampus / Q000187:drug effects; D026121:Indole Alkaloids / Q000494:pharmacology* / Q000627:therapeutic use; D008694:Methamphetamine / Q000378:metabolism* / Q000494:pharmacology; D000078183:Oxindoles; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D032070:Uncaria / Q000737:chemistry
chemical_list: D026121:Indole Alkaloids; D000078183:Oxindoles; D010936:Plant Extracts; D016760:Proto-Oncogene Proteins c-fos; D008694:Methamphetamine; C052714:rhyncophylline; D050882:CREB-Binding Protein; C494365:Crebbp protein, rat
keywords: Conditioned place preference; Methamphetamine; Rhynchophylline; c-Fos; p-CREB