title: Shotgun proteomics of plant plasma membrane and microdomain proteins using nano-LC-MS/MS.
abstract: Shotgun proteomics allows the comprehensive analysis of proteins extracted from plant cells, subcellular organelles, and membranes. Previously, two-dimensional gel electrophoresis-based proteomics was used for mass spectrometric analysis of plasma membrane proteins. In order to get comprehensive proteome profiles of the plasma membrane including highly hydrophobic proteins with a number of transmembrane domains, a mass spectrometry-based shotgun proteomics method using nano-LC-MS/MS for proteins from the plasma membrane proteins and plasma membrane microdomain fraction is described. The results obtained are easily applicable to label-free protein semiquantification.
authors.affiliation: United Graduate School of Agricultural Sciences, Iwate University, Morioka, Iwate, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Takahashi
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Takato
authors.initials: T
authors.lastname: Nakayama
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kawamura
authors.forename: Matsuo
authors.initials: M
authors.lastname: Uemura
mesh_terms: D018554:Avena / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D021962:Membrane Microdomains / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D036103:Nanotechnology; D010455:Peptides / Q000302:isolation & purification; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D040901:Proteomics / Q000379:methods*; D014357:Trypsin / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D010455:Peptides; D010940:Plant Proteins; D014357:Trypsin