title: Structural analysis and cross-protective efficacy of recombinant 87 kDa outer membrane protein (Omp87) of Pasteurella multocida serogroup B:2.
abstract: Pasteurella multocida serogroup B:2, a causative agent of haemorrhagic septicaemia (HS) in cattle and buffalo especially in tropical regions of Asian and African countries, is known to possess several outer membrane proteins (OMPs) as immunogenic antigens. In the present study, omp87 gene encoding for 87 kDa OMP (Omp87) protein of P. multocida serogroup B:2 strain P52, has been amplified (∼2304 bp), cloned in to pET32a vector and over-expressed in recombinant Escherichia coli as fusion protein. The recombinant Omp87 protein (∼102 kDa) including N-terminus hexa-histidine tag was purified under denaturing condition. Immunization of mice with rOmp87 resulted in increased antigen specific IgG titres in serum and provided protection of 66.6 and 83.3% following homologous (B:2) and heterologous (A:1) challenge, respectively. A homology model of Omp87 revealed the presence of two distinct domains; N-terminal domain with four POTRA repeats in the periplasmic space and a pore forming C-terminal β-barrel domain (β1- β16) in the outer membrane of P. multocida, which belong to Omp85-TpsB transporter superfamily of OMPs. The study indicated the potential possibilities to use rOmp87 protein along with suitable adjuvant in developing subunit vaccine for haemorrhagic septicaemia and pasteurellosis in livestock.
authors.affiliation: Clinical Bacteriology Laboratory, Indian Veterinary Research Institute (IVRI), Mukteswar 263138, Nainital (District), Uttarakhand (UK), India.
authors.forename: Abhinendra
authors.initials: A
authors.lastname: Kumar
authors.forename: Revanaiah
authors.initials: R
authors.lastname: Yogisharadhya
authors.forename: Muthannan A
authors.initials: MA
authors.lastname: Ramakrishnan
authors.forename: K N
authors.initials: KN
authors.lastname: Viswas
authors.forename: Sathish B
authors.initials: SB
authors.lastname: Shivachandra
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D056738:Cross Protection / Q000276:immunology; D006483:Hemorrhagic Septicemia / Q000276:immunology*; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D016979:Pasteurella multocida / Q000276:immunology*; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D016415:Sequence Alignment; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D022223:Vaccines, Subunit
keywords: Cross protection; Haemorrhagic septicaemia; Immunogenicity; Pasteurella multocida; Recombinant Omp87; Structural model