title: Symbiotic lactobacilli stimulate gut epithelial proliferation via Nox-mediated generation of reactive oxygen species.
abstract: The resident prokaryotic microbiota of the metazoan gut elicits profound effects on the growth and development of the intestine. However, the molecular mechanisms of symbiotic prokaryotic-eukaryotic cross-talk in the gut are largely unknown. It is increasingly recognized that physiologically generated reactive oxygen species (ROS) function as signalling secondary messengers that influence cellular proliferation and differentiation in a variety of biological systems. Here, we report that commensal bacteria, particularly members of the genus Lactobacillus, can stimulate NADPH oxidase 1 (Nox1)-dependent ROS generation and consequent cellular proliferation in intestinal stem cells upon initial ingestion into the murine or Drosophila intestine. Our data identify and highlight a highly conserved mechanism that symbiotic microorganisms utilize in eukaryotic growth and development. Additionally, the work suggests that specific redox-mediated functions may be assigned to specific bacterial taxa and may contribute to the identification of microbes with probiotic potential.
authors.affiliation: Epithelial Pathobiology Unit, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, USA.
authors.forename: Rheinallt M
authors.initials: RM
authors.lastname: Jones
authors.forename: Liping
authors.initials: L
authors.lastname: Luo
authors.forename: Courtney S
authors.initials: CS
authors.lastname: Ardita
authors.forename: Arena N
authors.initials: AN
authors.lastname: Richardson
authors.forename: Young Man
authors.initials: YM
authors.lastname: Kwon
authors.forename: Jeffrey W
authors.initials: JW
authors.lastname: Mercante
authors.forename: Ashfaqul
authors.initials: A
authors.lastname: Alam
authors.forename: Cymone L
authors.initials: CL
authors.lastname: Gates
authors.forename: Huixia
authors.initials: H
authors.lastname: Wu
authors.forename: Phillip A
authors.initials: PA
authors.lastname: Swanson
authors.forename: J David
authors.initials: JD
authors.lastname: Lambeth
authors.forename: Patricia W
authors.initials: PW
authors.lastname: Denning
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Neish
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D004330:Drosophila / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology*; D006657:Histones / Q000378:metabolism; D054884:Host-Pathogen Interactions; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology* / Q000382:microbiology; D007778:Lactobacillus / Q000472:pathogenicity; D007814:Larva / Q000166:cytology* / Q000378:metabolism / Q000382:microbiology; D051379:Mice; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D000074624:NADPH Oxidase 1; D010084:Oxidation-Reduction; D010766:Phosphorylation; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000382:microbiology; D013559:Symbiosis
chemical_list: D006657:Histones; D017382:Reactive Oxygen Species; D009247:NADH, NADPH Oxidoreductases; D000074624:NADPH Oxidase 1; C000614711:NOX1 protein, mouse