title: Separation of sulfoalkylated cyclodextrins with hydrophilic interaction liquid chromatography.
abstract: Determination of the charged state distributions of partially- and fully-substituted sulfoalkylated cyclodextrins was achieved using hydrophilic interaction liquid chromatography (HILIC). HILIC analysis of a spiked sample of the partially sulfopropylated cyclodextrins was achieved using a gradient to baseline resolve the charged states from -1 to -14. The fully-substituted CDs yielded a major peak with some trace impurities and the partially-substituted sulfopropylated cyclodextrins showed a wide range of charge states present in the mixture. Small changes in the structure of the cyclodextrins have a significant impact on the retention times of the various types of cyclodextrins investigated.
authors.affiliation: Department of Chemistry and Biochemistry, University of Alaska Fairbanks, Fairbanks, AK 99775-6160, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Jaramillo
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Kirschner
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Dai
authors.forename: Thomas K
authors.initials: TK
authors.lastname: Green
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D003505:Cyclodextrins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D008970:Molecular Weight
chemical_list: D003505:Cyclodextrins
keywords: Hydrophilic interaction liquid chromatography; Sulfoalkylated cyclodextrins