title: Detection of high-risk human papillomavirus subtypes in cervical glandular neoplasia by in situ hybridization.
abstract: In situ hybridization (ISH) was performed on paraffin-embedded tissues to detect multiple high-risk human papillomavirus (HPV) subtypes in 27 cases of cervical adenocarcinoma in situ (AIS) and adenocarcinoma (CA) specimens. These results were compared with those of HPV detection by HPV-PCR genotyping and p16 immunohistochemistry in the same specimens. Of the 27 cases, 17 (63%) showed HPV-DNA by HPV-ISH, including 3 metastatic lesions. HPV-DNA was detected in 18 cases (67%) by PCR. The concordance rate between HPV-ISH and HPV-PCR genotyping was 74% with moderate agreement (Kappa value, 0.41). HPV-16 was identified in 5 cases, HPV-18 in 2 cases, and HPV-45 in 1 case. Combining the results of HPV-ISH and HPV-PCR/genotyping, 22 cases (81.5%) were considered HPV positive. Immunohistochemical staining of p16 indicated that 25 (93%) cases were positive; however, 4 of these cases were HPV-negative by both PCR and ISH. Combining HPV-ISH and HPV-PCR/genotyping techniques demonstrated a high sensitivity of HPV detection in FFPE tissues from cervical glandular neoplasias. In contrast, p16 immunohistochemistry seemed to have a low specificity for determining HPV status in cervical glandular neoplasia. HPV-ISH is useful for recognizing the distribution of HPV in AIS and CA tissues and visualizing signal patterns, and may be a useful tool to confirm the cervical origin of neoplasias and metastatic lesions.
authors.affiliation: Department of Pathology and Laboratory Medicine, Kanazawa Medical University Kahoku, Ishikawa, Japan.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Sheng
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Minato
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Sasagawa
authors.forename: Satoko
authors.initials: S
authors.lastname: Nakada
authors.forename: Eriko
authors.initials: E
authors.lastname: Kinoshita
authors.forename: Nozomu
authors.initials: N
authors.lastname: Kurose
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nojima
authors.forename: Satoru
authors.initials: S
authors.lastname: Makinoda
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002278:Carcinoma in Situ / Q000473:pathology / Q000821:virology*; D002584:Cervix Uteri / Q000473:pathology / Q000821:virology*; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology*; D002578:Uterine Cervical Dysplasia / Q000473:pathology / Q000821:virology*
keywords: PCR; Uterine cervical cancer; adenocarcinoma; human papillomavirus; in situ hybridization; p16INK4A