title: Phenolic profile and antioxidant activity of Serbian polyfloral honeys.
abstract: A total of 58 polyfloral honey samples from different regions in Serbia were studied to determine their phenolic profile, total phenolic content and antioxidant capacity. UHPLC-LTQ OrbiTrap MS made possible the identification of 36 compounds: 24 flavonoids, two abscisic acids, and 10 phenolic acids and their derivatives. Quantification was done using 14 available standards. Data on phenolics and abscisic acids allowed the discrimination and classification of honeys in accordance to their geographical origin, using pattern recognition techniques, principal component analysis and partial least squares discriminant analysis. Samples originated from Vojvodina and Zlatibor region were clearly distinguished from those from the rest of Serbia because of the presence of dicaffeoylquinic acid, ellagic acid, caffeic acid phenethyl ester, and chlorogenic acid, among others. A good correlation (r = 0.865) was observed between total phenolic content and radical-scavenging activity. Total phenolic content ranged from 0.03 to 1.39 mg GAE/g and radical scavenging activity ranged from 1.31% to 25.61%.
authors.affiliation: Faculty of Chemistry, University of Belgrade, P.O. Box 51, 11158 Belgrade, Serbia.
authors.forename: Uroš
authors.initials: U
authors.lastname: Gašić
authors.forename: Silvio
authors.initials: S
authors.lastname: Kečkeš
authors.forename: Dragana
authors.initials: D
authors.lastname: Dabić
authors.forename: Jelena
authors.initials: J
authors.lastname: Trifković
authors.forename: Dušanka
authors.initials: D
authors.lastname: Milojković-Opsenica
authors.forename: Maja
authors.initials: M
authors.lastname: Natić
authors.forename: Zivoslav
authors.initials: Z
authors.lastname: Tešić
mesh_terms: D000040:Abscisic Acid / Q000032:analysis; D000975:Antioxidants / Q000032:analysis*; D002109:Caffeic Acids / Q000032:analysis; D002726:Chlorogenic Acid / Q000032:analysis; D004610:Ellagic Acid / Q000032:analysis; D005419:Flavonoids / Q000032:analysis; D005504:Food Analysis; D006722:Honey / Q000032:analysis*; D062385:Hydroxybenzoates / Q000032:analysis; D010636:Phenols / Q000032:analysis*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000032:analysis; D011801:Quinic Acid / Q000031:analogs & derivatives / Q000032:analysis; D055771:Serbia
chemical_list: D000975:Antioxidants; D002109:Caffeic Acids; D005419:Flavonoids; D062385:Hydroxybenzoates; D010636:Phenols; C472707:caffeoylquinic acid; D011801:Quinic Acid; D004610:Ellagic Acid; D002726:Chlorogenic Acid; D000040:Abscisic Acid; C055494:caffeic acid phenethyl ester; C017616:phenolic acid; D010626:Phenylethyl Alcohol
keywords: Geographical origin; Pattern recognition methods; Phenolic profile; Polyfloral honey; UHPLC-LTQ OrbiTrap MS