title: Molecular mechanism of strigolactone perception by DWARF14.
abstract: Strigolactones (SLs) are phytohormones that inhibit shoot branching and function in the rhizospheric communication with symbiotic fungi and parasitic weeds. An α/β-hydrolase protein, DWARF14 (D14), has been recognized to be an essential component of plant SL signalling, although its precise function remains unknown. Here we present the SL-dependent interaction of D14 with a gibberellin signalling repressor SLR1 and a possible mechanism of phytohormone perception in D14-mediated SL signalling. D14 functions as a cleavage enzyme of SLs, and the cleavage reaction induces the interaction with SLR1. The crystal structure of D14 shows that 5-hydroxy-3-methylbutenolide (D-OH), which is a reaction product of SLs, is trapped in the catalytic cavity of D14 to form an altered surface. The D14 residues recognizing D-OH are critical for the SL-dependent D14-SLR1 interaction. These results provide new insight into crosstalk between gibberellin and SL signalling pathways.
authors.affiliation: 1] Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan [2].
authors.forename: Hidemitsu
authors.initials: H
authors.lastname: Nakamura
authors.forename: You-Lin
authors.initials: YL
authors.lastname: Xue
authors.forename: Takuya
authors.initials: T
authors.lastname: Miyakawa
authors.forename: Feng
authors.initials: F
authors.lastname: Hou
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Qin
authors.forename: Kosuke
authors.initials: K
authors.lastname: Fukui
authors.forename: Xuan
authors.initials: X
authors.lastname: Shi
authors.forename: Emi
authors.initials: E
authors.lastname: Ito
authors.forename: Shinsaku
authors.initials: S
authors.lastname: Ito
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Miyauchi
authors.forename: Atsuko
authors.initials: A
authors.lastname: Asano
authors.forename: Naoya
authors.initials: N
authors.lastname: Totsuka
authors.forename: Takashi
authors.initials: T
authors.lastname: Ueda
authors.forename: Masaru
authors.initials: M
authors.lastname: Tanokura
authors.forename: Tadao
authors.initials: T
authors.lastname: Asami
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005875:Gibberellins / Q000737:chemistry / Q000378:metabolism; D006867:Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000737:chemistry / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D058441:Rhizosphere; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
chemical_list: D005875:Gibberellins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011994:Recombinant Proteins; D006867:Hydrolases; D015107:4-Butyrolactone