title: Activation of proteinase-activated receptor 2 prevents apoptosis of lung cancer cells.
abstract: The therapeutics of lung cancer (LC) is unsatisfactory. The pathogenesis of LC remains unclear. Protease-activated receptors (PAR) are involved in the immunoregulation. The present study aims to investigate the activation of PAR2 in regulation of the expression of EGFR and apoptosis of LC cells. The results showed that exposure to tryptase increased EGFR expression in A549 cells and suppressed the cell apoptosis. Tryptase also decreased the expression of Bax and increased Bcl-xL levels in A549 cells. We conclude that activation of PAR2 by tryptase can decrease the ratio of Bax/Bcl-xL and reduce the LC cell line, A549 cells, and apoptosis.
authors.affiliation: Departments of 1 Cardiothoracic Surgery,2Anesthesiology, and3Medical Oncology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Shao-hong
authors.initials: SH
authors.lastname: Huang
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Hui-guo
authors.initials: HG
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Rong
authors.forename: Sheng
authors.initials: S
authors.lastname: Ye
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D044464:Receptor, PAR-2 / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D053802:Tryptases / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D051020:bcl-X Protein / Q000378:metabolism
chemical_list: C494826:BAX protein, human; C494811:BCL2L1 protein, human; D012333:RNA, Messenger; D044464:Receptor, PAR-2; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; C512478:EGFR protein, human; D066246:ErbB Receptors; D053802:Tryptases