title: StageTip-based HAMMOC, an efficient and inexpensive phosphopeptide enrichment method for plant shotgun phosphoproteomics.
abstract: Phosphopeptide enrichment is the most critical step for successful LC-MS/MS-based shotgun phosphoproteomics. Recent technological improvements have made selective phosphopeptide enrichment from non-fractionated whole cell lysate digests with a single-step procedure possible. Here, a handy protocol is described for phosphopeptide enrichment from plant materials using hydroxy acid-modified metal oxide chromatography (HAMMMOC) with a stop-and-go-extraction tip (StageTip).
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Nakagami
mesh_terms: D002845:Chromatography / Q000295:instrumentation* / Q000379:methods*; D010748:Phosphopeptides / Q000302:isolation & purification*; D010750:Phosphoproteins / Q000302:isolation & purification*; D010940:Plant Proteins / Q000302:isolation & purification*; D040901:Proteomics / Q000295:instrumentation* / Q000379:methods*
chemical_list: D010748:Phosphopeptides; D010750:Phosphoproteins; D010940:Plant Proteins