title: Rapid interaction of Helicobacter pylori with microvilli of the polar human gastric epithelial cell line NCI-N87.
abstract: Infection with Helicobacter pylori results often in chronic gastritis, gastric ulcers or even gastric tumor development. Little is known about the initial interaction between gastric epithelial cells and H. pylori. The aim of the present study was to analyze the initial host contact to the bacteria. Monolayers of the human gastric epithelial cell line NCI-N87 grown on porous membranes were used and the apical side of the epithelium was exposed to the H. pylori wild-type strain P1 for 1 hr. Many epithelial cells were colonized by bacteria within the period of 60 min. Using scanning electron microscopy we detected that the bacteria were in close contact with the epithelia via microvilli. Further, transmission electron microscopy of the contact sites revealed no difference in the morphology of the microvilli in comparison to those not attached to the bacteria. The present study demonstrates the importance of microvilli on apical epithelial cells during the initial contact of the host by colonizing H. pylori.
authors.affiliation: Institute of Anatomy, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany.
authors.forename: Anne-Kathrin
authors.initials: AK
authors.lastname: Diesing
authors.forename: Constanze
authors.initials: C
authors.lastname: Nossol
authors.forename: Heidi
authors.initials: H
authors.lastname: Faber-Zuschratter
authors.forename: Werner
authors.initials: W
authors.lastname: Zuschratter
authors.forename: Lydia
authors.initials: L
authors.lastname: Renner
authors.forename: Olga
authors.initials: O
authors.lastname: Sokolova
authors.forename: Michael
authors.initials: M
authors.lastname: Naumann
authors.forename: Hermann-Josef
authors.initials: HJ
authors.lastname: Rothkötter
mesh_terms: D002478:Cells, Cultured; D017097:Electric Impedance; D004847:Epithelial Cells / Q000378:metabolism* / Q000382:microbiology / Q000648:ultrastructure; D005753:Gastric Mucosa / Q000378:metabolism*; D016481:Helicobacter Infections / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008871:Microvilli / Q000378:metabolism* / Q000382:microbiology / Q000648:ultrastructure; D013270:Stomach / Q000382:microbiology / Q000648:ultrastructure
keywords: gastric epithelial cells; scanning electron microscopy; tight junction; trans epithelial electrical resistance