title: Characterisation of lipid fraction of marine macroalgae by means of chromatography techniques coupled to mass spectrometry.
abstract: In this work the characterisation of the lipid fraction of several species of marine macro algae gathered along the eastern coast of Sicily is reported. Two species of green marine algae (Chloropyceae), two species of red marine algae (Rhodophyceae) and four species of brown marine algae (Pheophyceae) were evaluated in terms of fatty acids, triacylglycerols, pigments and phospholipids profile. Advanced analytical techniques were employed to fully characterise the lipid profile of these Mediterranean seaweeds, such as GC-MS coupled to a novel mass spectra database supported by the simultaneous use of linear retention index (LRI) for the identification of fatty acid profile; LC-MS was employed for the identification of triacylglycerols (TAGs), carotenoids and phospholipids; the determination of accurate mass was carried out on carotenoids and phospholipids. Quantitative data are reported on fatty acids and triacylglycerols as relative percentage of total fraction.
authors.affiliation: Dipartimento di Scienze del Farmaco e dei Prodotti per la Salute (SCIFAR), University of Messina, viale Annunziata, 98168 Messina, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Ragonese
authors.forename: Laura
authors.initials: L
authors.lastname: Tedone
authors.forename: Marco
authors.initials: M
authors.lastname: Beccaria
authors.forename: Germana
authors.initials: G
authors.lastname: Torre
authors.forename: Filomena
authors.initials: F
authors.lastname: Cichello
authors.forename: Francesco
authors.initials: F
authors.lastname: Cacciola
authors.forename: Paola
authors.initials: P
authors.lastname: Dugo
authors.forename: Luigi
authors.initials: L
authors.lastname: Mondello
mesh_terms: D002338:Carotenoids / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry; D000460:Chlorophyta / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004041:Dietary Fats / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry; D005410:Flame Ionization; D005504:Food Analysis / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008522:Mediterranean Sea; D009753:Nutritive Value; D000459:Phaeophyceae / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry; D010860:Pigments, Biological / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry; D000461:Rhodophyta / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D012624:Seaweed / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D012802:Sicily; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D014280:Triglycerides / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D010743:Phospholipids; D010860:Pigments, Biological; D014280:Triglycerides; D002338:Carotenoids
keywords: Carotenoids; Fatty acids; Phospholipids; Seaweeds; Triacylglycerols