title: Cellular localization of the atypical isoforms of protein kinase C (aPKCζ/PKMζ and aPKCλ/ι) on the neuromuscular synapse.
abstract: Several classic and novel protein kinase C (PKC) isoforms are selectively distributed in specific cell types of the adult neuromuscular junction (NMJ), in the neuron, glia and muscle components, and are involved in many functions, including neurotransmission. Here, we investigate the presence in this paradigmatic synapse of atypical PKCs, full-length atypical PKC zeta (aPKCζ), its separated catalytic part (PKMζ) and atypical lambda-iota PKC (aPKCλ/ι). High resolution immunohistochemistry was performed using a pan-atypical PKC antibody. Our results show moderate immunolabeling on the three cells (presynaptic motor nerve terminal, teloglial Schwann cell and postsynaptic muscle cell) suggesting the complex involvement of atypical PKCs in synaptic function.
authors.affiliation: Unitat d'Histologia i Neurobiologia (UHN), Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Sant Llorenç 21, 43201 Reus, Spain.
authors.forename: Núria
authors.initials: N
authors.lastname: Besalduch
authors.forename: Maria A
authors.initials: MA
authors.lastname: Lanuza
authors.forename: Neus
authors.initials: N
authors.lastname: Garcia
authors.forename: Teresa
authors.initials: T
authors.lastname: Obis
authors.forename: Manel M
authors.initials: MM
authors.lastname: Santafe
authors.forename: Marta
authors.initials: M
authors.lastname: Tomàs
authors.forename: Mercedes
authors.initials: M
authors.lastname: Priego
authors.forename: Josep
authors.initials: J
authors.lastname: Tomàs
mesh_terms: D000818:Animals; D007527:Isoenzymes / Q000378:metabolism*; D032342:Muscle Cells / Q000201:enzymology; D018482:Muscle, Skeletal / Q000201:enzymology / Q000294:innervation; D009469:Neuromuscular Junction / Q000201:enzymology*; D011493:Protein Kinase C / Q000378:metabolism*; D051744:Protein Kinase C-epsilon / Q000378:metabolism*; D051381:Rats; D012583:Schwann Cells / Q000201:enzymology; D000099293:Protein Kinase C-lambda
chemical_list: D007527:Isoenzymes; D011493:Protein Kinase C; D051744:Protein Kinase C-epsilon; D000099293:Protein Kinase C-lambda
keywords: CaC; DAG; Immunohistochemistry; LAL; LTP; Levator auris longus; NMJ; Nerve terminals; Neuromuscular junction; PKC; PKC isoforms; PKM ζ; PMA; PS; STS; Western blot; aPKC ζ; aPKCλ/ι; atypical lambda-iota; calphostin C; diacylglycerol; full length atypical PKC zeta; long-term potentiation; neuromuscular junction; phorbol esters; phosphatidylserine; protein kinase C; protein kinase M ζ; semithin section