title: How to use 2D gel electrophoresis in plant proteomics.
abstract: Two-dimensional electrophoresis has nurtured the birth of proteomics. It is however no longer the exclusive setup used in proteomics, with the development of shotgun proteomics techniques that appear more fancy and fashionable nowadays.Nevertheless, 2D gel-based proteomics still has valuable features, and sometimes unique ones, which make it often an attractive choice when a proteomics strategy must be selected. These features are detailed in this chapter, as is the rationale for selecting or not 2D gel-based proteomics as a proteomic strategy.
authors.affiliation: Chemistry and Biology of Metals, CNRS UMR 5249, Grenoble, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Rabilloud
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D010940:Plant Proteins / Q000378:metabolism; D010944:Plants / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D010940:Plant Proteins