title: Serum and skin levels of miR-369-3p in patients with psoriasis and their correlation with disease severity.
abstract: BACKGROUND
  Psoriasis is a common autoimmune skin disease, characterized by intense proliferation and abnormal differentiation of keratinocytes. Recently, some miRNAs have been proven to show aberrant expression in psoriasis and play a role in the pathogenesis of the disease.
  
  
  OBJECTIVE
  The aim of this study was to detect serum and skin miR-369-3p levels in patients with psoriasis and confirm their correlation with disease severity. The regulatory mechanism between miR-369-3p and TNF-α was also investigated.
  
  
  METHODS
  Bioinformatics analysis for target genes of miRNAs was performed using multiple prediction software. Serum samples and skin tissues were collected and serum and skin miR-369-3p levels were measured. The Psoriasis Area Severity Index scores of patients and the correlation with serum and skin miR-369-3p levels were evaluated. Transient transfection and Elisa were applied to HaCaT cells to testify the relationship between expression of miR-369-3p and TNF-α.
  
  
  RESULTS
  Serum and skin miR-369-3p levels were both higher in patients with psoriasis than those in healthy controls (P<0.05; P<0.001, respectively). And miR-369-3p levels in skin had a positive linear relation with PASI scores in psoriasis patients (r = 0.70, P<0.05). Unexpectedly, miR-369-3p failed to show a regulatory effect on TNF-α levels in HaCaT cells.
  
  
  CONCLUSIONS
  The expression of miR-369-3p is increased in both serum samples and skin tissues from psoriasis patients, and its level in the skin positively correlates with disease severity. Further studies are needed to clarify the role of miR-369-3p in the pathogenesis of psoriasis.
authors.affiliation: Department of Dermatology, Xijing Hospital, Fourth Military Medical University, 127 Changlexi Road, Xi'an, Shaanxi, 710032, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Guo
authors.forename: Weigang
authors.initials: W
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Wei
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Guannan
authors.initials: G
authors.lastname: Zhu
authors.forename: Qiong
authors.initials: Q
authors.lastname: Shi
authors.forename: Shuli
authors.initials: S
authors.lastname: Li
authors.forename: Rui
authors.initials: R
authors.lastname: Ge
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Gao
authors.forename: Tianwen
authors.initials: T
authors.lastname: Gao
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.forename: Chunying
authors.initials: C
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D015603:Keratinocytes; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D011565:Psoriasis / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D012720:Severity of Illness Index; D012867:Skin / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: C560308:MIRN369 microRNA, human; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha
keywords: Psoriasis Area Severity Index; miR-369-3p; microRNAs; psoriasis; tumor necrosis factor-α