title: Water-compatible poly (hydroxyethyl methacrylate) polymer sorbent for miniaturized syringe assisted extraction of sulfonamides in milk.
abstract: A simple, convenient, and economic self-assembly miniaturized syringe assisted extraction (mini-SAE) using poly (hydroxyethyl methacrylate) polymer (PHEMA) as sorbent coupled with liquid chromatography was proposed for rapid screening of sulfadiazine (SD) and sulfamonomethoxine (SMM) in milk. The water-compatible PHEMA was synthesized by reversible addition-fragmentation chain transfer precipitation polymerization using trithiocarbonate as chain transfer agent and methanol-water system as reaction medium. The obtained PHEMA sorbent showed good affinity to sulfonamides and was successfully applied as a special sorbent for a mini-SAE device for simultaneous extraction and isolation of SD and SMM in milk samples. Under the optimum condition, good linearity was obtained in a range of 7.0-700 ng g(-1) (r≥0.9995) and the average recoveries of SD and SMM at three spiked levels were ranged from 85.6 to 100.3% with the relative standard deviations (RSD) ≤6.5%. The presented PHEMA-mini-SAE protocol could be potentially applied as an alternative tool for analyzing the residues of SAs in complicated biological samples.
authors.affiliation: Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding, 071002, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Gao
authors.forename: Hongyuan
authors.initials: H
authors.lastname: Yan
authors.forename: Ning
authors.initials: N
authors.lastname: Sun
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D008892:Milk / Q000737:chemistry*; D008904:Miniaturization; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D052616:Solid Phase Extraction; D013411:Sulfadiazine / Q000032:analysis* / Q000302:isolation & purification; D013422:Sulfamonomethoxine / Q000032:analysis* / Q000302:isolation & purification; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D013411:Sulfadiazine; D011102:Polyhydroxyethyl Methacrylate; D013422:Sulfamonomethoxine
keywords: Milk; Miniaturized syringe assisted extraction; PHEMA sorbent; Sulfonamides