title: T cell activation is determined by the number of presented antigens.
abstract: Antigen recognition is a key event during T cell activation. Here, we introduce nanopatterned antigen arrays that mimic the antigen presenting cell surface during T cell activation. The assessment of activation related events revealed the requirement of a minimal density of 90-140 stimulating major histocompatibility complex class II proteins (pMHC) molecules per μm(2). We demonstrate that these substrates induce T cell responses in a pMHC dose-dependent manner and that the number of presented pMHCs dominates over local pMHC density.
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems , Heisenbergstraße 3, D-70569 Stuttgart, Germany.
authors.forename: Janosch
authors.initials: J
authors.lastname: Deeg
authors.forename: Markus
authors.initials: M
authors.lastname: Axmann
authors.forename: Jovana
authors.initials: J
authors.lastname: Matic
authors.forename: Anastasia
authors.initials: A
authors.lastname: Liapis
authors.forename: David
authors.initials: D
authors.lastname: Depoil
authors.forename: Jehan
authors.initials: J
authors.lastname: Afrose
authors.forename: Silvia
authors.initials: S
authors.lastname: Curado
authors.forename: Michael L
authors.initials: ML
authors.lastname: Dustin
authors.forename: Joachim P
authors.initials: JP
authors.lastname: Spatz
mesh_terms: D000938:Antigen-Presenting Cells / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology*; D008285:Major Histocompatibility Complex / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology; D053758:Nanoparticles / Q000737:chemistry*; D013569:Synapses / Q000276:immunology; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology*
chemical_list: D008565:Membrane Proteins