title: Assessment of SOX11 expression in routine lymphoma tissue sections: characterization of new monoclonal antibodies for diagnosis of mantle cell lymphoma.
abstract: The diagnosis of mantle cell lymphoma (MCL) can be difficult, especially when no t(11;14) translocation and cyclin D1 overexpression can be detected. In such cases, the transcription factor SOX11 represents an important diagnostic marker, as it is expressed in most MCLs and, in particular, in all cyclin D1-negative MCLs reported so far. A reliable anti-SOX11 antibody is therefore a very useful tool for routine diagnosis. Here, we characterize the new monoclonal anti-SOX11 antibodies, suitable for Western blot assay and immunohistochemistry (IHC) on formalin-fixed paraffin-embedded tissue; we tested them on a large series of primary lymphoid tumors and compared these results with those of other routinely used antibodies. Moreover, we show that IHC results depend on transcription levels of SOX11, which suggests that posttranscriptional and posttranslational modifications do not significantly affect cutoff levels for IHC detection of SOX11.
authors.affiliation: *Hematopathology Unit, Pathology Department, Hospital Clínic ‡Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) §Department of Anatomic Pathology, Pharmacology and Microbiology, University of Barcelona, Barcelona, Spain †Institute of Surgical Pathology, University Hospital Zurich, Zurich, Switzerland ∥Research and Development, Atlas Antibodies, Stockholm, Sweden.
authors.forename: Davide
authors.initials: D
authors.lastname: Soldini
authors.forename: Alexandra
authors.initials: A
authors.lastname: Valera
authors.forename: Carla
authors.initials: C
authors.lastname: Solé
authors.forename: Jara
authors.initials: J
authors.lastname: Palomero
authors.forename: Virginia
authors.initials: V
authors.lastname: Amador
authors.forename: José Ignacio
authors.initials: JI
authors.lastname: Martin-Subero
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Ribera-Cortada
authors.forename: Cristina
authors.initials: C
authors.lastname: Royo
authors.forename: Itziar
authors.initials: I
authors.lastname: Salaverria
authors.forename: Sílvia
authors.initials: S
authors.lastname: Beà
authors.forename: Elena
authors.initials: E
authors.lastname: Gonzalvo
authors.forename: Henrik
authors.initials: H
authors.lastname: Johannesson
authors.forename: Mileyka
authors.initials: M
authors.lastname: Herrera
authors.forename: Lluís
authors.initials: L
authors.lastname: Colomo
authors.forename: Antonio
authors.initials: A
authors.lastname: Martinez
authors.forename: Elias
authors.initials: E
authors.lastname: Campo
mesh_terms: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D015153:Blotting, Western; D019938:Cyclin D1 / Q000032:analysis; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D020522:Lymphoma, Mantle-Cell / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000473:pathology; D011237:Predictive Value of Tests; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055751:SOXC Transcription Factors / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D014158:Transcription, Genetic
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; C530104:CCND1 protein, human; D012333:RNA, Messenger; C097473:SOX11 protein, human; D055751:SOXC Transcription Factors; D019938:Cyclin D1