title: Biophysically defined and cytocompatible covalently adaptable networks as viscoelastic 3D cell culture systems.
abstract: Presented here is a cytocompatible covalently adaptable hydrogel uniquely capable of mimicking the complex biophysical properties of native tissue and enabling natural cell functions without matrix degradation. Demonstrated is both the ability to control elastic modulus and stress relaxation time constants by more than an order of magnitude while predicting these values based on fundamental theoretical understanding and the simulation of muscle tissue and the encapsulation of myoblasts.
authors.affiliation: Department of Chemical and Biological Engineering, University of Colorado Boulder, Jennie Smoly Caruthers Biotechnology Building, 3415 Colorado Ave, 596 UCB, Boulder, CO, 80303; BioFrontiers Institute, University of Colorado Boulder, Jennie Smoly Caruthers Biotechnology Building, 3415 Colorado Ave, 596 UCB, Boulder, CO, 80303.
authors.forename: Daniel D
authors.initials: DD
authors.lastname: McKinnon
authors.forename: Dylan W
authors.initials: DW
authors.lastname: Domaille
authors.forename: Jennifer N
authors.initials: JN
authors.lastname: Cha
authors.forename: Kristi S
authors.initials: KS
authors.lastname: Anseth
mesh_terms: D000447:Aldehydes / Q000737:chemistry; D000818:Animals; D001547:Benzaldehydes / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D055592:Biophysical Phenomena; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival; D055119:Elastic Modulus; D006835:Hydrazones / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D007700:Kinetics; D051379:Mice; D032342:Muscle Cells / Q000502:physiology; D018482:Muscle, Skeletal / Q000502:physiology; D032446:Myoblasts / Q000166:cytology / Q000502:physiology; D011092:Polyethylene Glycols / Q000737:chemistry; D013816:Thermodynamics; D054457:Tissue Scaffolds / Q000737:chemistry*; D055682:Viscoelastic Substances / Q000737:chemistry*
chemical_list: D000447:Aldehydes; D001547:Benzaldehydes; D001672:Biocompatible Materials; D006835:Hydrazones; D020100:Hydrogels; D055682:Viscoelastic Substances; D011092:Polyethylene Glycols; C029915:4-nitrobenzaldehyde; C018475:butyraldehyde; C032175:benzaldehyde
keywords: dynamic materials; hydrogels; polymeric materials; self-healing materials; supramolecular chemistry