title: Production of chimeric adenovirus.
abstract: The use of chimeric pseudotyped vectors is a common way to modify the adenoviral tropism by replacing the fiber protein. In this chapter the procedure to generate a chimeric adenovirus pre-stock from a plasmid containing the adenoviral genome is described. Also, the chimeric adenovirus replicative cycle to increase the yield in further productions is determined. Finally, two different protocols, in culture plates and in suspension cultures, to produce the virus at large scale are also detailed.
authors.affiliation: Department of Biochemistry and Molecular Biology, Center of Animal Biotechnology and Gene Therapy (CBATEG), Universitat Autonoma Barcelona, Bellaterra, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Miralles
authors.forename: Marc
authors.initials: M
authors.lastname: Garcia
authors.forename: Marcos
authors.initials: M
authors.lastname: Tejero
authors.forename: Assumpció
authors.initials: A
authors.lastname: Bosch
authors.forename: Miguel
authors.initials: M
authors.lastname: Chillón
mesh_terms: D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018929:Cell Culture Techniques; D002460:Cell Line; D005822:Genetic Vectors / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D011995:Recombination, Genetic; D019562:Viral Load; D014779:Virus Replication