title: Detection of respiratory viruses using a multiplex real-time PCR assay in Germany, 2009/10.
abstract: The aim of this study was to determine the prevalence of respiratory viruses and to prospectively evaluate the performance of the fast-track diagnostics (FTD) respiratory pathogens multiplex PCR assay shortly after the 2009/10 influenza pandemic. Highly sensitive monoplex real-time PCR assays served as references. Discrepant results were further analyzed by the xTAG RVP Fast assay. A total of 369 respiratory samples from children and adults were collected prospectively in Germany from December 2009 until June 2010. The sensitivity and specificity of the FTD assay after resolution of discrepant results was 92.2 % and 99.5 %, respectively. Lowest specificity of the FTD assay was observed for human bocavirus. Multiple detections were recorded in 33/369 (8.9 %) of the samples by monoplex PCR and in 43/369 (11.7 %) using the FTD assay. The most prevalent viruses were respiratory syncytial virus and human metapneumovirus. Only pandemic influenza virus A/H1N1 (2009), and not seasonal influenza virus, was detected. Viruses other than influenza virus accounted for the majority of acute respiratory infections. The FTD assay can be easily implemented in general diagnostic laboratories and facilitate the optimization of patient-management schemes.
authors.affiliation: Institute of Virology, University Medical Centre, Hermann-Herder Str. 11, 79104, Freiburg, Germany.
authors.forename: Sibylle
authors.initials: S
authors.lastname: Bierbaum
authors.forename: Johannes
authors.initials: J
authors.lastname: Forster
authors.forename: Reinhard
authors.initials: R
authors.lastname: Berner
authors.forename: Gerta
authors.initials: G
authors.lastname: Rücker
authors.forename: Gernot
authors.initials: G
authors.lastname: Rohde
authors.forename: Dieter
authors.initials: D
authors.lastname: Neumann-Haefelin
authors.forename: Marcus
authors.initials: M
authors.lastname: Panning
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D005858:Germany / Q000453:epidemiology; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D015995:Prevalence; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000453:epidemiology* / Q000821:virology; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*; D014780:Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D055815:Young Adult