title: Molecular phylogenetic analysis of dominant microbial populations in aged refuse.
abstract: The phylogenetic analysis of dominant microbial populations in 8-year-old refuse samples was done in terms of the whole Bacterial and Archaeal domains. The results indicated that the Bacterial 16S rRNA genes sequences from the aged refuse were largely affiliated with the genus Bacillus, and that more than 60 % of the Archaeal sequences were closely related to the methanogenic archaeon. Some inferentially identified extremophilic organisms, particularly alkaliphiles and/or halophiles, were noted to be present in the aged refuse. Moreover, molecular evidence for the occurrence of ammonia-oxidizing Archaea in aged refuse was reported, which opens up avenues for elucidating its role in ammonia transformation in landfill systems. It seems reasonable to assume that the highly complex environment within the landfill systems may select for microbial populations with versatile metabolism and strong adaptation. These findings underline the need for further biochemical and ecological study of these organisms in aged refuse.
authors.affiliation: Shanghai Key Laboratory of Urbanization and Ecological Restoration, School of Resources and Environment Science, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, China, yhe@des.ecnu.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Li
authors.forename: Liping
authors.initials: L
authors.lastname: Yao
authors.forename: You Cai
authors.initials: YC
authors.lastname: Zhao
authors.forename: Min Sheng
authors.initials: MS
authors.lastname: Huang
authors.forename: Gong Ming
authors.initials: GM
authors.lastname: Zhou
mesh_terms: D000368:Aged; D001105:Archaea / Q000145:classification* / Q000302:isolation & purification*; D001419:Bacteria / Q000145:classification* / Q000302:isolation & purification*; D044822:Biodiversity; D016000:Cluster Analysis; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D020459:Genes, rRNA; D006801:Humans; D010802:Phylogeny; D019642:RNA, Archaeal / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014866:Waste Products
chemical_list: D019641:DNA, Archaeal; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D019642:RNA, Archaeal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014866:Waste Products