title: Biodegradable self-assembled nanoparticles of poly (D,L-lactide-co-glycolide)/hyaluronic acid block copolymers for target delivery of docetaxel to breast cancer.
abstract: To develop biodegradable docetaxel-loaded self-assembled nanoparticles of poly (D,L-lactide-co-glycolide)/hyaluronic acid block copolymers were successfully synthesized. These copolymers could form nanoparticles with small size (<200 nm), an acceptable CMC (~7.9 mg/L), typical core/shell structure and superior stability in one week. DTX-loaded PLGA(502H)-b-HA(5.6k) nanoparticles (DTX/SANPs) showed a biphasic release pattern within 120 h, and exhibited enhanced cytotoxicity toward CD44-overexpressing MDA-MB-231 cells. Cellular uptake study indicated that PLGA(502H)-b-HA(5.6k) nanoparticles (SANPs) were taken up in MDA-MB-231 cells by CD44-mediated endocytosis. Pharmacokinetics study revealed DTX/SANPs could prolong the circulation of DTX in the blood. In vivo studies demonstrated that SANPs exhibited enhanced tumor targeting and antitumor activity with lower systemic toxicity. In conclusion, DTX/SANPs have great potential for targeted chemotherapy for CD44-overexpressing breast cancer.
authors.affiliation: Department of Pharmaceutical Science, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China.
authors.forename: Jingbin
authors.initials: J
authors.lastname: Huang
authors.forename: He
authors.initials: H
authors.lastname: Zhang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yu
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.forename: Guoqing
authors.initials: G
authors.lastname: Zhang
authors.forename: Guichen
authors.initials: G
authors.lastname: Zhou
authors.forename: Junjie
authors.initials: J
authors.lastname: Liu
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Sun
authors.forename: Duxin
authors.initials: D
authors.lastname: Sun
authors.forename: Ying
authors.initials: Y
authors.lastname: Lu
authors.forename: Yanqiang
authors.initials: Y
authors.lastname: Zhong
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D001672:Biocompatible Materials; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D000077143:Docetaxel; D004337:Drug Carriers; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D018960:Hyaluronan Receptors / Q000276:immunology; D006820:Hyaluronic Acid / Q000008:administration & dosage* / Q000737:chemistry; D019344:Lactic Acid / Q000008:administration & dosage* / Q000737:chemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D053758:Nanoparticles; D011100:Polyglycolic Acid / Q000008:administration & dosage* / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017207:Rats, Sprague-Dawley; D043823:Taxoids / Q000008:administration & dosage / Q000627:therapeutic use*
chemical_list: D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D004337:Drug Carriers; D018960:Hyaluronan Receptors; D043823:Taxoids; D000077143:Docetaxel; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D006820:Hyaluronic Acid
keywords: Breast cancer; CD44; Docetaxel; Hyaluronic acid; Poly (d,l-lactide-co-glycolide); Self-assembled nanoparticles