title: Electrophoresis-chemiluminescence detection of phenols catalyzed by hemin.
abstract: Based on the catalytic activity of hemin, an efficient biocatalyst, an indirect capillary electrophoresis-chemiluminescence (CE-CL) detection method for phenols using a hemin-luminol-hydrogen peroxide system was developed. Through a series of static injection experiments, hemin was found to perform best in a neutral solution rather than an acidic or alkaline medium. Although halide ions such as Br(-) and F(-) could further enhance the CL signal catalyzed by hemin, it is difficult to apply these conditions to this CE-CL detection system because of the self-polymerization of hemin, as it hinders the CE process. The addition of concentrated ammonium hydroxide to an aqueous/dimethyl sulfoxide solution of hemin-luminol afforded a stable CE-CL baseline. The indirect CE-CL detection of five phenols using this method gave the following limits of detections: 4.8 × 10(-8) mol/L (o-sec-butylphenol), 4.9 × 10(-8) mol/L (o-cresol), 5.4 × 10(-8) mol/L (m-cresol), 5.3 × 10(-8) mol/L (2,4-dichlorophenol) and 7.1 × 10(-8) mol/L (phenol).
authors.affiliation: Department of Chemistry, East China Normal University, Shanghai, 200062, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Shu
authors.forename: Jinkun
authors.initials: J
authors.lastname: Zhu
authors.forename: Qingjiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Pingang
authors.initials: P
authors.lastname: He
authors.forename: Yuzhi
authors.initials: Y
authors.lastname: Fang
mesh_terms: D002384:Catalysis; D019075:Electrophoresis, Capillary; D006427:Hemin / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D049449:Luminescence; D008165:Luminol / Q000737:chemistry; D010636:Phenols / Q000032:analysis*
chemical_list: D010636:Phenols; D008165:Luminol; D006427:Hemin; D006861:Hydrogen Peroxide
keywords: capillary electrophoresis; chemiluminescence; hemin; phenol detection