title: Plant heat shock protein 90 as carrier-adjuvant for immunization against a reporter antigen.
abstract: Here, we evaluated the modulation of the immune response induced by Hsp90 of Nicotiana benthamiana (NbHsp90.3) against the Maltose Binding Protein (MBP) as a reporter antigen. Equimolar quantities of recombinant proteins were administered in mice as follows: MBP alone (MBP group), a mixture of MBP and rNbHsp90.3 (MBP+rNbHsp90.3 group) and the fusion of MBP to rNbHsp90.3 (MBP-rNbHsp90.3 group). The covalent linkage between NbHsp90.3 and MBP to bring a fusion protein was essential to induce the strong specific antibody response with predominance of IgG2a. Eighty-four days after the first immunization, splenocyte proliferation from MBP-rNbHsp90.3-immunized mice was consistently higher than that from MBP and MBP+rNbHsp90.3 groups. In addition, splenocytes from MBP-rNbHsp90.3 immunized mice produced higher levels of IFN-γ than controls. Finally, both formulations with rNbHsp90.3 significantly enhanced the MHC class I expression levels, but only rNbHsp90.3 covalent bound to MBP induced a specific cellular immune response against MBP measured as increased percentage of CD8(+) T cells. Taken together, these results suggest that plant HSP90s could be incorporated as adjuvants in vaccines that require the generation of a Th1 response along with a CD8 cytotoxic cell response to confer immunity.
authors.affiliation: Laboratorio de Biotecnología Vegetal, IIB-INTECH, CONICET-UNSAM, Chascomús, Provincia de Buenos Aires, Argentina.
authors.forename: Mariana G
authors.initials: MG
authors.lastname: Corigliano
authors.forename: Ignacio
authors.initials: I
authors.lastname: Fenoy
authors.forename: Valeria
authors.initials: V
authors.lastname: Sander
authors.forename: Andrea
authors.initials: A
authors.lastname: Maglioco
authors.forename: Alejandra
authors.initials: A
authors.lastname: Goldman
authors.forename: Marina
authors.initials: M
authors.lastname: Clemente
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D005260:Female; D005805:Genes, MHC Class I; D018841:HSP90 Heat-Shock Proteins / Q000276:immunology*; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000276:immunology; D058619:Maltose-Binding Proteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010940:Plant Proteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014026:Nicotiana / Q000737:chemistry*
chemical_list: D000276:Adjuvants, Immunologic; D018841:HSP90 Heat-Shock Proteins; D007074:Immunoglobulin G; D058619:Maltose-Binding Proteins; D010940:Plant Proteins; D011994:Recombinant Proteins; D007371:Interferon-gamma
keywords: Adjuvant; Immunity; Plant Hsp90; Vaccine