title: Intestinal Peyer's patch-immunomodulating glucomannans from rhizomes of Anemarrhena asphodeloides Bunge.
abstract: During screening for intestinal Peyer's patch-immunomodulating polysaccharides from plant resources including medicinal herbs, a potent modulating activity was observed in a crude polysaccharide fraction (AS-1) from the rhizome of Anemarrhena asphodeloides Bunge. Oral administration of AS-1 (100 mg/kg/day) to aged BALB/c mice enhanced productions of IL-10, IFN-γ and IL-6 from Peyer's patch immunocompetent cells, and its oral administration to ovalbumin (OVA)-fed B10.A mice led to significant suppression on induction of OVA-specific IgE in systemic immune system. Further fractionation of the polysaccharides in the crude polysaccharide fraction, AS-1, yielded 4 polysaccharide fractions that were potently active, and contained glucomannans. Treatment of these polysaccharide fractions with endo-β-D-(1→4)-mannanase significantly decreased their activities. Mannanase digestion of the active glucomannan gave both long and short hexosyl-oligosaccharides, whereas konjac glucomannan, which was inactive, released short oligosaccharides. Structural analysis indicates that the long oligosaccharides from the active glucomannan contain mannanase-resistant complex structure comprising β-D-Man and β-D-Glc.
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, Tokyo 108-8641, Japan; Graduate School of Infection Control Sciences, Kitasato University, Tokyo 108-8641, Japan; Oriental Medicine Research Center, Kitasato University, Tokyo 108-8641, Japan. Electronic address: kiyohara@lisci.kitasato-u.ac.jp.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kiyohara
authors.forename: Toshiake
authors.initials: T
authors.lastname: Matsuzaki
authors.forename: Haruki
authors.initials: H
authors.lastname: Yamada
mesh_terms: D031403:Anemarrhena / Q000737:chemistry*; D000818:Animals; D005260:Female; D006801:Humans; D015850:Interleukin-6; D007422:Intestines / Q000737:chemistry*; D007564:Japan; D008351:Mannans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000187:drug effects / Q000276:immunology*; D010946:Plants, Medicinal / Q000737:chemistry / Q000187:drug effects; D027343:Rhizome / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015850:Interleukin-6; D008351:Mannans; C022901:(1-6)-alpha-glucomannan
keywords: Anemarrhena asphodeloides Bunge; Peyer’s patches; β-d-(1→4)-Glucomannan