title: Targeted proteomics strategy applied to biomarker evaluation.
abstract: The evaluation of biomarker candidates, involving quantitative measurement of a large number of proteins in bodily fluids, remains the main obstruction in the development of a biomarker validation pipeline. Although immunoassays are commonly used, high-throughput and multiplex-capable methods are required for expediting the evaluation process. MS-based approaches employing targeted proteomic strategies provide not only a sensitive, but in addition a precise quantification tool, which is versatile, systematic, and scalable. Its capability of multiplexing hundreds of targets facilitate a cost-effective and rapid evaluation and is especially useful during the early stage of the process where a large list of candidate biomarkers must be triaged before entering validation studies. The robustness requirement for the methods also mandates a high degree of selectivity to analyze complex clinical samples. Improvement in the selectivity of LC-MS methods has been achieved by adopting high-resolution and high-accuracy mass analyzers to perform quantitative analyses with a novel method called parallel reaction monitoring. This article discusses the design and performance of biomarker evaluation studies using targeted proteomics strategies and the implementation of recent technology developments.
authors.affiliation: Luxembourg Clinical Proteomics Center, CRP-Santé, Strassen, Luxembourg.
authors.forename: Yeoun Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Sebastien
authors.initials: S
authors.lastname: Gallien
authors.forename: Jan
authors.initials: J
authors.lastname: van Oostrum
authors.forename: Bruno
authors.initials: B
authors.lastname: Domon
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D006801:Humans; D013058:Mass Spectrometry; D040901:Proteomics / Q000379:methods*
chemical_list: D015415:Biomarkers
keywords: Parallel reaction monitoring; Targeted proteomics