title: Injectable thermosensitive PEG-PCL-PEG hydrogel/acellular bone matrix composite for bone regeneration in cranial defects.
abstract: We have certified that the injectable thermosensitive ABM/PECE composite presented promising potential in bone regeneration benefited from the incorporation of the intrinsic osteoinductive acellular bone matrix (ABM) granules into the poly(ethylene glycol)-poly(ε-capro-lactone)-poly(ethylene glycol) (PEG-PCL-PEG, PECE) hydrogel. In this study, the 12 mm × 8 mm × 2 mm cranial defects of the New Zealand white rabbits were fabricated to evaluate the bone regeneration effect. The ABM/PECE composite was injected into the defect while the pure PECE as control, and the bone regeneration was evaluated at 4, 12 and 20 weeks post-surgery by X-radiological examination, micro-computed tomography examination and histological analysis. In ABM/PECE composite treated group, the new bone formed originally from both the margin and the center of the defect, and the defect region had healed up to 20 weeks. Furthermore, the shadow density of the newly formed bone eventually approximated to host cortical bone. In comparison, the control group was filled with sparing new bone with low-density only from the periphery of the defect. Meanwhile, the quantitative determination of new bone by histomorphometry confirmed the excellent bone regeneration of ABM/PECE composite. All the results suggested that the ABM/PECE composite presented enhanced bone regeneration guidance in rabbit cranial defects.
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, PR China.
authors.forename: PeiYan
authors.initials: P
authors.lastname: Ni
authors.forename: QiuXia
authors.initials: Q
authors.lastname: Ding
authors.forename: Min
authors.initials: M
authors.lastname: Fan
authors.forename: JinFeng
authors.initials: J
authors.lastname: Liao
authors.forename: ZhiYong
authors.initials: Z
authors.lastname: Qian
authors.forename: JingCong
authors.initials: J
authors.lastname: Luo
authors.forename: XiuQun
authors.initials: X
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Luo
authors.forename: ZhiMing
authors.initials: Z
authors.lastname: Yang
authors.forename: YuQuan
authors.initials: Y
authors.lastname: Wei
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001861:Bone Regeneration; D002152:Calorimetry, Differential Scanning; D011091:Polyesters / Q000008:administration & dosage*; D011092:Polyethylene Glycols / Q000008:administration & dosage*; D011817:Rabbits; D012886:Skull / Q000002:abnormalities*; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C502908:poly(ethylene glycol)-poly(caprolactone)-poly(ethylene glycol); D011092:Polyethylene Glycols
keywords: Acellular bone matrix/PEG–PCL–PEG (ABM/PECE); Bone regeneration; Cranial defects; Injectable; Thermosensitive hydrogel