title: Different surgical techniques and L-carnitine supplementation in an experimental varicocele model.
abstract: We aimed to investigate the impact of various varicocelectomy techniques and/or L-carnitine as an adjunct treatment, following the emergence of oxidative stress, on the expression levels of SCF/c-kit signalling pathways in spermatogenesis. Forty-two rats were divided into seven groups: group 1 (G1) control; group 2 (G2) sham; group 3 (G3) varicocele; group 4 (G4) varicocele + varicocelectomy with testicular nonartery sparing; group 5 (G5) same as G4 but with artery sparing; group 6 (G6) same as G4 but with L-carnitine and group 7 (G7) same as G5 with L-carnitine. mRNA expression levels of SCF and c-kit were measured quantitatively using real-time polymerase chain reaction. CASP-3 activity at protein level was determined, and histological evaluation was performed. mRNA expression level of SCF increased in G6 as compared to control group (3.52-folds change; P = 0.035), whereas mRNA expression level of c-kit gene remained the same. We found that in the left testis of G6 group, mRNA expression level of SCF increased 2.2-folds in comparison with the right testis (P < 0.05). There were no statistically significant differences in the CASP-3 protein expression levels between the control and other groups. When Cosentino Score analyses of immunostaining were conducted, we observed no significant differences among groups. Spermatogenic failure could be primarily due to a sertoli cell dysfunction. Although surgical treatment has been the best option for management of varicocele, auxiliary agents like L-carnitine may be considered as supportive treatment regimes in addition to conventional surgical treatments.
authors.affiliation: Faculty of Medicine, Department of Urology, Gazi University, Ankara, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Akdemir
authors.forename: S
authors.initials: S
authors.lastname: Gurocak
authors.forename: E
authors.initials: E
authors.lastname: Konac
authors.forename: I
authors.initials: I
authors.lastname: Ure
authors.forename: H I
authors.initials: HI
authors.lastname: Onen
authors.forename: I I
authors.initials: II
authors.lastname: Gonul
authors.forename: S
authors.initials: S
authors.lastname: Sozen
authors.forename: A
authors.initials: A
authors.lastname: Menevse
mesh_terms: D000818:Animals; D002331:Carnitine / Q000627:therapeutic use*; D017024:Chemotherapy, Adjuvant; D008297:Male; D018384:Oxidative Stress; D011897:Random Allocation; D017208:Rats, Wistar; D013091:Spermatogenesis; D019089:Stem Cell Factor / Q000378:metabolism*; D013521:Urologic Surgical Procedures, Male / Q000379:methods*; D014646:Varicocele / Q000188:drug therapy / Q000601:surgery*; D014803:Vitamin B Complex / Q000627:therapeutic use*
chemical_list: D019089:Stem Cell Factor; D014803:Vitamin B Complex; D002331:Carnitine
keywords: Caspase-3; L-carnitine; SCF/c-kit; gene expression; varicocele