title: Antifibrotic effects of immobilized hyaluronidase in repeated bleomycin-induced lesions of the alveolar epithelium.
abstract: Antifibrotic activity of testicular hyaluronidase, immobilized on polyethylenoxide and obtained by electron beam synthesis, was studied on the model of bleomycin injuries to the alveolar epithelium (irreversible pneumofibrosis) in C57Bl/6 mice and compared to the effect of testicular hyaluronidase. Intranasal therapy with immobilized and testicular hyaluronidases prevented the deposition of fibrotic mass in the parenchyma of "bleomycin" lungs. The effect of immobilized hyaluronidase was more pronounced than that of testicular hyaluronidase. The studied compounds were virtually inessential for infiltration of the alveolar interstitium and alveolar tracts by lymphocytes, macrophages, neutrophils, and plasma cells. Unchanged histoarchitectonics of bleomycin-damaged lungs in immobilized hyaluronidase therapy was due to suppression of the progenitor fibroblast cells (CD45(-)).
authors.affiliation: Institute of Pharmacology, Siberian Division of the Russian Academy of Medical Sciences, Tomsk; Scientific Future Management, Novosibirsk, Russia. ovpershina@gmail.com.
authors.forename: A M
authors.initials: AM
authors.lastname: Dygai
authors.forename: E G
authors.initials: EG
authors.lastname: Skurikhin
authors.forename: N N
authors.initials: NN
authors.lastname: Ermakova
authors.forename: A M
authors.initials: AM
authors.lastname: Reztsova
authors.forename: O V
authors.initials: OV
authors.lastname: Pershina
authors.forename: E S
authors.initials: ES
authors.lastname: Khmelevskaya
authors.forename: V A
authors.initials: VA
authors.lastname: Krupin
authors.forename: I E
authors.initials: IE
authors.lastname: Stepanova
authors.forename: V M
authors.initials: VM
authors.lastname: Reztsova
authors.forename: A V
authors.initials: AV
authors.lastname: Artamonov
authors.forename: A A
authors.initials: AA
authors.lastname: Bekarev
authors.forename: P G
authors.initials: PG
authors.lastname: Madonov
authors.forename: D N
authors.initials: DN
authors.lastname: Kinsht
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000737:chemistry; D001761:Bleomycin; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000008:administration & dosage / Q000737:chemistry; D006821:Hyaluronoglucosaminidase / Q000008:administration & dosage* / Q000737:chemistry; D054990:Idiopathic Pulmonary Fibrosis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D011095:Polyethylenes / Q000737:chemistry; D011650:Pulmonary Alveoli / Q000473:pathology; D020545:Respiratory Mucosa / Q000473:pathology
chemical_list: D000893:Anti-Inflammatory Agents; D004337:Drug Carriers; D004800:Enzymes, Immobilized; D011095:Polyethylenes; D001761:Bleomycin; D006821:Hyaluronoglucosaminidase