title: Ginsenoside Rb1 attenuates oxygen-glucose deprivation-induced apoptosis in SH-SY5Y cells via protection of mitochondria and inhibition of AIF and cytochrome c release.
abstract: To investigate the role of mitochondria in the protective effects of ginsenoside Rb1 on cellular apoptosis caused by oxygen-glucose deprivation, in this study, MTT assay, TUNEL staining, flow cytometry, immunocytochemistry and western blotting were used to examine the cellular viability, apoptosis, ROS level, mitochondrial membrane potential, and the distribution of apoptosis inducing factor, cytochrome c, Bax and Bcl-2 in nucleus, mitochondria and cytoplasm. We found that pretreatment with GRb1 improved the cellular viability damaged by OGD. Moreover, GRb1 inhibited apoptosis in SH-SY5Y cells induced by OGD. Further studies showed that the elevation of cellular reactive oxygen species levels and the reduction of mitochondrial membrane potential caused by OGD were both counteracted by GRb1. Additionally, GRb1 not only suppressed the translocation of apoptosis inducing factor into nucleus and cytochrome c into cytoplasm, but also inhibited the increase of Bax within mitochondria and alleviated the decrease of mitochondrial Bcl-2. Our study indicates that the protection of GRb1 on OGD-induced apoptosis in SH-SY5Y cells is associated with its protection on mitochondrial function and inhibition of release of AIF and cytochrome c.
authors.affiliation: Department of Pediatrics, First Hospital of Jilin University, Changchun 130021, China. pengfeige@gmail.com.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Liang
authors.forename: Ying
authors.initials: Y
authors.lastname: Yu
authors.forename: Boyu
authors.initials: B
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Lu
authors.forename: Jizhou
authors.initials: J
authors.lastname: Zhang
authors.forename: Hongbo
authors.initials: H
authors.lastname: Zhang
authors.forename: Pengfei
authors.initials: P
authors.lastname: Ge
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D051033:Apoptosis Inducing Factor / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D045304:Cytochromes c / Q000378:metabolism*; D004353:Drug Evaluation, Preclinical; D036145:Ginsenosides / Q000494:pharmacology*; D005947:Glucose / Q000502:physiology; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology*; D018696:Neuroprotective Agents / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: C494829:AIFM1 protein, human; D051033:Apoptosis Inducing Factor; D036145:Ginsenosides; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; C442759:ginsenoside Rb1; D045304:Cytochromes c; D005947:Glucose