title: Low-abundance envelope protein VP12 of white spot syndrome virus interacts with envelope protein VP150 and capsid protein VP51.
abstract: VP12 and VP150 are two minor envelope proteins of white spot syndrome virus (WSSV). In our previous studies, VP12 was found to co-migrate with 53-kDa form of VP150 on two-dimensional Blue Native/SDS-PAGE, suggesting that there is an interaction between them. In this study, we confirmed the interaction by co-immunoprecipitation assay and demonstrated that the binding region with VP12 is located between residues 207 and 803 of VP150. Further studies found that VP12 can be attached to WSSV capsids by interacting with capsid protein VP51. These findings suggest that VP12 may function as a linker protein participating in the linkage between VP12/VP150 complex and viral nucleocapsid.
authors.affiliation: College of Ocean & Earth Sciences, Xiamen University, Xiamen 361005, China; State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources of State Oceanic Administration; Third Institute of Oceanography, Xiamen 361005, China. Electronic address: lijianbo326@163.com.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Li
authors.forename: Limei
authors.initials: L
authors.lastname: Xu
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Yang
mesh_terms: D036022:Capsid Proteins / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D047468:Immunoprecipitation; D011485:Protein Binding; D025941:Protein Interaction Mapping; D014759:Viral Envelope Proteins / Q000378:metabolism*; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D036022:Capsid Proteins; D014759:Viral Envelope Proteins
keywords: Interaction; VP12; VP150; VP51; WSSV