title: Transcription profile of chemokine receptors, cytokines and cytotoxic markers in peripheral blood of dogs with epitheliotropic cutaneous lymphoma.
abstract: BACKGROUND
  Mycosis fungoides (MF) is the most common form of canine epitheliotropic cutaneous lymphoma, which is characterized by the accumulation of neoplastic CD8(+) T cells. Given that multifocal skin lesions are commonly seen in MF, neoplastic lymphocytes may actively migrate into the blood circulation.
  
  
  OBJECTIVE
  Cytotoxic T cells with a skin-homing phenotype could be increased in the blood circulation of dogs with MF.
  
  
  METHODS
  Ten dogs with MF and 10 age-matched healthy dogs were included.
  
  
  METHODS
  The transcription levels of chemokine receptors, cytokines and cytotoxic markers in peripheral blood of dogs with MF were quantified by real-time RT-PCR.
  
  
  RESULTS
  The dogs with MF had lower transcription levels of chemokine receptors associated with skin homing (CCR4), epitheliotropism (CXCR3), lymph node homing (CCR7), a type-1 cytokine (LT-α) and cytotoxic markers (perforin and granzyme B) in the circulation than healthy control dogs (P < 0.05).
  
  
  CONCLUSIONS
  The present results suggest that the number of peripheral cytotoxic T cells with a skin-homing phenotype could be decreased in the peripheral blood of dogs with MF, which might be due to the sequestration of cytotoxic T cells in the lesional skin.
authors.affiliation: The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Chimura
authors.forename: Aki
authors.initials: A
authors.lastname: Iio
authors.forename: Eiji
authors.initials: E
authors.lastname: Ozaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Mori
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ito
authors.forename: Nobuo
authors.initials: N
authors.lastname: Murayama
authors.forename: Masahiko
authors.initials: M
authors.lastname: Nagata
authors.forename: Kaori
authors.initials: K
authors.lastname: Ide
authors.forename: Koji
authors.initials: K
authors.lastname: Nishifuji
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kamishina
authors.forename: Sadatoshi
authors.initials: S
authors.lastname: Maeda
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004283:Dog Diseases / Q000097:blood / Q000378:metabolism*; D004285:Dogs; D015972:Gene Expression Regulation, Neoplastic; D009182:Mycosis Fungoides / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D019707:Receptors, Chemokine / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D014408:Biomarkers, Tumor; D016207:Cytokines; D019707:Receptors, Chemokine