title: Hippocampal interleukin-1beta in the juvenile and middle-aged rat: response to chronic forced swim or high-light open-field stress stimulation.
abstract: It is postulated that stress differentially affects interleukin-1beta (IL-1beta) during ontogenetic life. This study examined the influence of chronic exposure to forced swim (FS) stress or high-light open-field (HL-OF) stress on interleukin-1beta (IL-1beta). The total level of IL-1beta protein was assessed by Western blot analysis of hippocampal extracts. Double immunofluorescence staining was used to reveal the percentage of IL-1beta/NeuN (NeuN - neuronal marker) cells in the CA1, CA3 and dentate gyrus (DG) hippocampal subfields. Juvenile (P28; P - postnatal day) and middle-aged (P360) rats were used in the experiment. The research showed no significant differences in IL-1beta protein levels between P28 and P360 non-stress rats. However, a substantial increase in the percentage of IL-1beta-ir neurons in the CA1, CA3 and DG in P360 rats was observed. Chronic FS had no significant influence on IL-1beta expression in the hippocampus or on the percentage of IL-1beta-ir neurons in CA1, CA3 and DG hippocampal subfields in either age group. During HL-OF, the IL-1beta level was significantly increased in the hippocampus of P28 and P360 rats, whereas a marked increase in the percentage of IL-1beta-ir neurons in the CA1, CA3 and DG hippocampal areas occurred only in P360 animals. These results indicate that chronic HL-OF stimulation was the factor inducing changes in the IL-1beta protein levels in P28 and P360 rats and in the percentage of IL-1beta/NeuN-ir cells in the hippocampus of P360 animals.
authors.affiliation: Department of Anatomy and Neurobiology, ewabadowska@gumed.edu.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Badowska-Szalewska
authors.forename: Beata
authors.initials: B
authors.lastname: Ludkiewicz
authors.forename: Justyna
authors.initials: J
authors.lastname: Sidor-Kaczmarek
authors.forename: Grazyna
authors.initials: G
authors.lastname: Lietzau
authors.forename: Jan H
authors.initials: JH
authors.lastname: Spodnik
authors.forename: Dariusz
authors.initials: D
authors.lastname: Świetlik
authors.forename: Beata
authors.initials: B
authors.lastname: Domaradzka-Pytel
authors.forename: Janusz
authors.initials: J
authors.lastname: Moryś
mesh_terms: D000375:Aging; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D004195:Disease Models, Animal; D005106:Exploratory Behavior / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D006624:Hippocampus / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism*; D008027:Light; D008297:Male; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013315:Stress, Psychological / Q000473:pathology*; D013550:Swimming / Q000523:psychology*
chemical_list: D053583:Interleukin-1beta; D010751:Phosphopyruvate Hydratase