title: A novel taspine derivative suppresses human liver tumor growth and invasionin vitroandin vivo.
abstract: Taspine is an attractive target of research due to the anticancer and anti-angiogenic effects shown byin vitroandin vivoexperiments. The present study investigated the role of tas1611, which is a derivative of taspine that has increased activity and solubility, in the regulation of the invasive properties of the SMMC-7721 liver cell linein vitroand in tumor inhibitionin vivo. The proliferation of the SMMC-7721 cells was examined using the tetrazole blue colorimetric method. Matrigel®invasion chamber assays and zymogram analyses were performed to assess the inhibitory effect of tas1611 on cell invasion. Finally, a solid tumor athymic mouse model was employed to further investigate the anti-tumor effect of this compound. The results revealed that tas1611 had a marked inhibitory effect on the invasion of the SMMC-7721 cells and that this effect was associated with the activity and expression levels of matrix metalloproteinase (MMP)-2 and MMP-9. Furthermore, tas1611 was able to inhibit tumor growth effectively in a solid tumor SMMC-7721 athymic mouse model. In conclusion, tas1611 may serve as a promising novel therapeutic candidate for the treatment of metastatic liver cancer.
authors.affiliation: School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Zheng
authors.forename: Yingzhuan
authors.initials: Y
authors.lastname: Zhan
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Zhang
keywords: invasion; liver tumor; matrix metalloproteinases; tas1611