title: Wohlfahrtiimonas larvae sp. nov., isolated from the larval gut of Hermetia illucens (Diptera: Stratiomyidae).
abstract: A novel, Gram-negative, facultative anaerobic, motile and short rod-shaped bacterium, strain KBL006(T) was isolated from the larval gut of Hermetia illucens, Black soldier fly. The 16S rRNA gene sequence of strain KBL006(T) showed 96.4 % similarity to that of Wohlfahrtiimonas chitiniclastica S5(T). Strain KBL006(T) grew optimally at 30 °C, at pH 8.0 and in the presence of 1-2 % (w/v) NaCl. Oxidase activity and catalase activity were positive. The major fatty acids were C18:1 ω7c, C14:0, and C16:0. The major respiratory quinone was ubiquinone-8 (Q-8). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol, and two phospholipids. The G+C content of the genomic DNA was 45.2 mol%. Based on these polyphasic data, strain KBL006(T) is considered to represent a novel species in the genus Wohlfahrtiimonas, for which the name Wohlfahrtiimonas larvae sp. nov. is proposed. The type strain is KBL006(T) (= KACC 16839(T) = JCM 18424(T)).
authors.affiliation: Department of Biology, Kyung Hee University, Seoul, 130-701, South Korea.
authors.forename: Jae Kook
authors.initials: JK
authors.lastname: Lee
authors.forename: Youn Yeop
authors.initials: YY
authors.lastname: Lee
authors.forename: Kwan Ho
authors.initials: KH
authors.lastname: Park
authors.forename: Jeonggu
authors.initials: J
authors.lastname: Sim
authors.forename: Youngcheol
authors.initials: Y
authors.lastname: Choi
authors.forename: Sung-Jae
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004175:Diptera / Q000254:growth & development / Q000382:microbiology*; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000382:microbiology; D007814:Larva / Q000254:growth & development / Q000382:microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D044166:Xanthomonadaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids