title: A key role of microRNA-29b for the suppression of colon cancer cell migration by American ginseng.
abstract: Metastasis of colon cancer cells increases the risk of colon cancer mortality. We have recently shown that American ginseng prevents colon cancer, and a Hexane extract of American Ginseng (HAG) has particularly potent anti-inflammatory and anti-cancer properties. Dysregulated microRNA (miR) expression has been observed in several disease conditions including colon cancer. Using global miR expression profiling, we observed increased miR-29b in colon cancer cells following exposure to HAG. Since miR-29b plays a role in regulating the migration of cancer cells, we hypothesized that HAG induces miR-29b expression to target matrix metalloproteinase-2 (MMP-2) thereby suppressing the migration of colon cancer cells. Results are consistent with this hypothesis. Our study supports the understanding that targeting MMP-2 by miR-29b is a mechanism by which HAG suppresses the migration of colon cancer cells.
authors.affiliation: Department of Drug Discovery and Biomedical Sciences, South Carolina College of Pharmacy, University of South Carolina, Columbia, South Carolina, United States of America.
authors.forename: Deepak
authors.initials: D
authors.lastname: Poudyal
authors.forename: Xiangli
authors.initials: X
authors.lastname: Cui
authors.forename: Phuong Mai
authors.initials: PM
authors.lastname: Le
authors.forename: Anne B
authors.initials: AB
authors.lastname: Hofseth
authors.forename: Anthony
authors.initials: A
authors.lastname: Windust
authors.forename: Mitzi
authors.initials: M
authors.lastname: Nagarkatti
authors.forename: Prakash S
authors.initials: PS
authors.lastname: Nagarkatti
authors.forename: Aaron J
authors.initials: AJ
authors.lastname: Schetter
authors.forename: Curtis C
authors.initials: CC
authors.lastname: Harris
authors.forename: Lorne J
authors.initials: LJ
authors.lastname: Hofseth
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics; D003110:Colonic Neoplasms / Q000235:genetics*; D045325:HCT116 Cells; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology
chemical_list: C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D010936:Plant Extracts; D020778:Matrix Metalloproteinase 2