title: Subcritical CO2 pretreatment of sugarcane bagasse and its enzymatic hydrolysis for sugar production.
abstract: The present work investigated the effects of subcritical CO2 pretreatment of sugarcane bagasse at different CO2 pressure, pretreatment time, and temperature with relative high-solid concentration (15% w/v) to the composition of prehydrolyzate and the enzymatic hydrolysis. The results indicated that the maximum xylose yields in prehydrolyzate liquid were 15.78 g (combined 3.16 g xylose and 12.62 g xylo-oligosaccharides per 100g raw material). Due to the effective removal of hemicellulose, the maximum glucose yield in enzyme hydrolyzate reached 37.99 g per 100g raw material, representing 91.87% of glucose in the sugarcane bagasse. The maximal total sugars yield (combined xylose and glucose both in prehydrolyzate and enzymatic hydrolyzate) were 52.95 g based on 100g raw material. These results indicated that subcritical CO2 pretreatment can effectively improve the enzymatic hydrolysis, so it could be successfully applied to sugarcane bagasse.
authors.affiliation: State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, PR China.
authors.forename: Hongdan
authors.initials: H
authors.lastname: Zhang
authors.forename: Shubin
authors.initials: S
authors.lastname: Wu
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000096:biosynthesis*; D002245:Carbon Dioxide / Q000494:pharmacology*; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis / Q000187:drug effects; D008031:Lignin / Q000378:metabolism; D031786:Saccharum / Q000737:chemistry*; D014994:Xylose / Q000378:metabolism
chemical_list: D002241:Carbohydrates; D002245:Carbon Dioxide; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D014994:Xylose; D002480:Cellulase; D005947:Glucose
keywords: Enzymatic hydrolysis; Subcritical CO(2) pretreatment; Sugarcane bagasse; Total sugar