title: Breaking in and grabbing a meal: Anaplasma phagocytophilum cellular invasion, nutrient acquisition, and promising tools for their study.
abstract: Anaplasma phagocytophilum invades neutrophils to cause the emerging infection, human granulocytic anaplasmosis. Here, we provide a focused review of the A. phagocytophilum invasin-host cell receptor interactions that promote bacterial entry and the degradative and membrane traffic pathways that the organism exploits to route nutrients to the organelle in which it resides. Because its obligatory intracellular nature hinders knock out-complementation approaches, we also discuss the current methods used to study A. phagocytophilum gene function and the potential benefit of applying novel tools that have advanced studies of other obligate intracellular bacterial pathogens.
authors.affiliation: Department of Microbiology and Immunology, Virginia Commonwealth University School of Medicine, Richmond, VA 23298-0678, USA.
authors.forename: Hilary K
authors.initials: HK
authors.lastname: Truchan
authors.forename: David
authors.initials: D
authors.lastname: Seidman
authors.forename: Jason A
authors.initials: JA
authors.lastname: Carlyon
mesh_terms: D041081:Anaplasma phagocytophilum / Q000472:pathogenicity / Q000502:physiology*; D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D016873:Ehrlichiosis / Q000378:metabolism / Q000382:microbiology; D054884:Host-Pathogen Interactions; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism / Q000382:microbiology; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D011956:Receptors, Cell Surface
keywords: Bacterial invasion; Ehrlichia; Nutritional virulence; Obligate intracellular; Rab GTPase; Rickettsia