title: 1p36.22 region containing PGD gene is frequently gained in human cervical cancer.
abstract: OBJECTIVE
  To identify commonly occurring DNA copy number alterations in Korean cervical cancers.
  
  
  METHODS
  DNA copy number alteration was screened by whole-genome array comparative genomic hybridization (CGH) analysis. For the array CGH discovery, genomic DNA from five cervical cancers and 10 normal cervical tissues were examined. For the independent validation of the most significant chromosomal alteration (1p36.22, PGD gene), 40 formalin-fixed paraffin-embedded cervical tissue samples were collected; 10 of them were used for quantitative polymerase chain reaction and the other 30 samples were used for immunohistochemical analysis. Chromosomal segments differently distributed between cancers and normal controls were determined to be recurrently altered regions (RAR).
  
  
  RESULTS
  A total of 13 RAR (11 RAR losses and two RAR gains) were defined in this study. Of the 13 cervical cancer-specific RAR, RAR gain in the 1p36.22 locus where the PGD gene is located was the most commonly detected in cancers (P = 0.004). In the quantitative polymerase chain reaction replication, copy number gain of the PGD gene was consistently identified in cervical cancers but not in the normal tissues (P = 0.02). In immunohistochemical analysis, PGD expression was significantly higher in cervical cancers than normal tissues (P = 0.02).
  
  
  CONCLUSIONS
  Our results will be helpful to understand cervical carcinogenesis, and the PGD gene can be a useful biomarker of cervical cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Kangdong Sacred Heart Hospital, Hallym University, Seoul, Korea.
authors.forename: Maria
authors.initials: M
authors.lastname: Lee
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Nam
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Jung
authors.forename: Soon Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Sung-Jong
authors.initials: SJ
authors.lastname: Lee
authors.forename: Joo Hee
authors.initials: JH
authors.lastname: Yoon
authors.forename: Nak Woo
authors.initials: NW
authors.lastname: Lee
authors.forename: Seob
authors.initials: S
authors.lastname: Jeon
authors.forename: Joong Sub
authors.initials: JS
authors.lastname: Choi
authors.forename: Chi-Heum
authors.initials: CH
authors.lastname: Cho
authors.forename: Yong
authors.initials: Y
authors.lastname: Moon
authors.forename: Yeun-Jun
authors.initials: YJ
authors.lastname: Chung
authors.forename: Yongil
authors.initials: Y
authors.lastname: Kwon
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D056915:DNA Copy Number Variations; D005260:Female; D018628:Gene Dosage; D006801:Humans; D008875:Middle Aged; D010734:Phosphogluconate Dehydrogenase / Q000032:analysis / Q000235:genetics*; D056910:Republic of Korea; D002583:Uterine Cervical Neoplasms / Q000737:chemistry / Q000235:genetics*
chemical_list: D010734:Phosphogluconate Dehydrogenase
keywords: PGD; array comparative genomic hybridization; cervical cancer; copy number alteration; recurrently altered region