title: Time-lapse imaging of developing meristems using confocal laser scanning microscope.
abstract: Analysis of shoot meristem shape and gene expression pattern has been conducted in many species over the past decades. Recent live imaging techniques have allowed an unprecedented accumulation of data on the biology of meristematic cells, as well as a better understanding of the molecular and biophysical mechanisms behind shape changes in this tissue. Here we describe in detail how to prepare shoot apices of both Arabidopsis and tomato, in order to image them over time using a confocal microscope equipped with a long-distance water-dipping lens.
authors.affiliation: Laboratoire de Reproduction et Développement des Plantes, INRA, CNRS, ENS, UCB Lyon 1, and Laboratoire Joliot Curie, CNRS, ENS, Université de Lyon, Lyon, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Hamant
authors.forename: Pradeep
authors.initials: P
authors.lastname: Das
authors.forename: Agata
authors.initials: A
authors.lastname: Burian
mesh_terms: D017360:Arabidopsis / Q000033:anatomy & histology / Q000254:growth & development; D018519:Meristem / Q000033:anatomy & histology* / Q000254:growth & development; D018613:Microscopy, Confocal; D059008:Time-Lapse Imaging / Q000379:methods*