title: Phenolic profiling of the skin, pulp and seeds of Albariño grapes using hybrid quadrupole time-of-flight and triple-quadrupole mass spectrometry.
abstract: This paper describes for the first time a complete characterisation of the phenolic compounds in different anatomical parts of the Albariño grape. The application of high-performance liquid chromatography coupled with two complementary techniques, hybrid quadrupole time-of-flight and triple-quadrupole mass spectrometry, allowed the phenolic composition of the Albariño grape to be unambiguously identified and quantified. A more complete phenolic profile was obtained by product ion and precursor ion scans, while a neutral loss scan at 152 u enabled a fast screening of procyanidin dimers, trimers and their galloylated derivatives. The compounds were confirmed by accurate mass measurements in QqToF-MS and QqToF-MS/MS modes at high resolution, and good fits were obtained for all investigated ions, with errors ranging from 0.2 to 4.5 mDa. To the best of our knowledge, two flavanol monomer hexosides were detected in the grape berry for the first time.
authors.affiliation: Nutrition and Food Science Department, XaRTA, INSA, Pharmacy School, University of Barcelona, Barcelona, Spain.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Di Lecce
authors.forename: Sara
authors.initials: S
authors.lastname: Arranz
authors.forename: Olga
authors.initials: O
authors.lastname: Jáuregui
authors.forename: Anna
authors.initials: A
authors.lastname: Tresserra-Rimbau
authors.forename: Paola
authors.initials: P
authors.lastname: Quifer-Rada
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Lamuela-Raventós
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry / Q000191:economics; D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates / Q000032:analysis / Q000737:chemistry; D019587:Dietary Supplements / Q000191:economics; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D005525:Food-Processing Industry / Q000191:economics; D005638:Fruit / Q000737:chemistry*; D055951:Functional Food / Q000032:analysis*; D006027:Glycosides / Q000032:analysis / Q000737:chemistry; D007220:Industrial Waste / Q000032:analysis / Q000191:economics; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D012639:Seeds / Q000737:chemistry*; D013030:Spain; D021241:Spectrometry, Mass, Electrospray Ionization; D013267:Stilbenes / Q000032:analysis / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis
chemical_list: D000975:Antioxidants; D002934:Cinnamates; D005419:Flavonoids; D006027:Glycosides; D007220:Industrial Waste; D010636:Phenols; D010936:Plant Extracts; D013267:Stilbenes
keywords: Accurate mass; Albariño grape; Flavanol hexose; Hybrid quadrupole time of flight; Neutral loss scan; Phenolic compounds