title: Olive baboons: a non-human primate model for testing dengue virus type 2 replication.
abstract: OBJECTIVE
  This study evaluated the use of a non-human primate, the olive baboon (Papio anubis), as a model of dengue infection. Olive baboons closely resemble humans genetically and physiologically and have been used extensively for assessing novel vaccine formulations.
  
  
  METHODS
  Two doses of dengue virus type 2 (DENV-2) were tested in baboons: 10(3) and 10(4) pfu. Similarly, African green monkeys received the same quantity of virus and acted as positive controls.
  
  
  RESULTS
  Following exposure, high levels of viremia were detected in both animal species. There was a trend to detect more days of viremia and more homogeneous viral titers in animals receiving the low viral dose. In addition, baboons infected with the virus generally exhibited positive virus isolation 1 day later than African green monkeys. Humoral responses consisting of antiviral and neutralizing antibodies were detected in all animals after infection.
  
  
  CONCLUSIONS
  We conclude that baboons provide an alternative non-human primate species for experimental DENV-2 infection and we recommend their use for further tests of vaccines, administering the lowest dose assayed: 10(3) pfu.
authors.affiliation: Center for Genetic Engineering and Biotechnology (CIGB), Avenue 31, PO Box 6162, Havana 6, 10 600, Cuba.
authors.forename: Iris
authors.initials: I
authors.lastname: Valdés
authors.forename: Lázaro
authors.initials: L
authors.lastname: Gil
authors.forename: Jorge
authors.initials: J
authors.lastname: Castro
authors.forename: Damián
authors.initials: D
authors.lastname: Odoyo
authors.forename: Rikoi
authors.initials: R
authors.lastname: Hitler
authors.forename: Elephas
authors.initials: E
authors.lastname: Munene
authors.forename: Yaremis
authors.initials: Y
authors.lastname: Romero
authors.forename: Lucy
authors.initials: L
authors.lastname: Ochola
authors.forename: Karelia
authors.initials: K
authors.lastname: Cosme
authors.forename: Thomas
authors.initials: T
authors.lastname: Kariuki
authors.forename: Gerardo
authors.initials: G
authors.lastname: Guillén
authors.forename: Lisset
authors.initials: L
authors.lastname: Hermida
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D001831:Body Temperature; D016663:Cercopithecinae; D002522:Chlorocebus aethiops; D003715:Dengue / Q000175:diagnosis / Q000276:immunology* / Q000821:virology*; D003716:Dengue Virus / Q000502:physiology*; D004195:Disease Models, Animal; D007074:Immunoglobulin G / Q000276:immunology; D009500:Neutralization Tests; D013997:Time Factors; D019562:Viral Load; D014766:Viremia / Q000276:immunology / Q000821:virology; D014779:Virus Replication
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D007074:Immunoglobulin G
keywords: African green monkeys; Baboons; Dengue; Non-human primates; Vaccine; Viremia