title: Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspring.
abstract: Carbamazepine (CBZ) is an anti-epileptic drug that acts on Leydig cells, affecting steroidogenesis and causes fetal malformation. The aim of this study was to investigate the effects of CBZ on male sexual maturation and other male parameters. Rat dams were treated with CBZ during pregnancy and breastfeeding. The anogenital distance (AGD) and the anogenital index (AGI) were obtained. Testicular descent and preputial separation were also evaluated. The offspring was euthanized at PND 41 and 63. The accessory glands were weighed and the testes were collected for histopathological, morphometric and sterological analyses. The numerical density of Leydig cells and hormone dosage were obtained. CBZ caused an increase of AGI and a delay of testicular descent and of preputial separation. CBZ also caused a decrease of testosterone level and of sperm count and an increase of abnormal sperm. These results indicate that CBZ delays puberty onset and affects steroidogenesis and sperm quality.
authors.affiliation: Laboratory of Developmental Biology, Department of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Rua Botucatu, 740, Ed. Lei tão da Cunha, 2° andar, CEP: 04023-900 Sao Paulo, SP, Brazil.
authors.forename: Rhayza Roberta
authors.initials: RR
authors.lastname: Andretta
authors.affiliation: Laboratory of Developmental Biology, Department of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Rua Botucatu, 740, Ed. Lei tão da Cunha, 2° andar, CEP: 04023-900 Sao Paulo, SP, Brazil.
authors.forename: Fatima Kazue
authors.initials: FK
authors.lastname: Okada
authors.affiliation: Laboratory of Developmental Biology, Department of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Rua Botucatu, 740, Ed. Lei tão da Cunha, 2° andar, CEP: 04023-900 Sao Paulo, SP, Brazil.
authors.forename: Camila Cicconi
authors.initials: CC
authors.lastname: Paccola
authors.affiliation: Laboratory of Developmental Biology, Department of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Rua Botucatu, 740, Ed. Lei tão da Cunha, 2° andar, CEP: 04023-900 Sao Paulo, SP, Brazil.
authors.forename: Taiza
authors.initials: T
authors.lastname: Stumpp
authors.affiliation: Laboratory of Developmental Biology, Department of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Rua Botucatu, 740, Ed. Lei tão da Cunha, 2° andar, CEP: 04023-900 Sao Paulo, SP, Brazil. Electronic address: samaraurban@gmail.com.
authors.forename: Samara Urban
authors.initials: SU
authors.lastname: de Oliva
authors.affiliation: Laboratory of Developmental Biology, Department of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Rua Botucatu, 740, Ed. Lei tão da Cunha, 2° andar, CEP: 04023-900 Sao Paulo, SP, Brazil. Electronic address: miraglia.sm@gmail.com.
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Miraglia
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000633:toxicity*; D002220:Carbamazepine / Q000633:toxicity*; D004958:Estradiol / Q000097:blood; D005260:Female; D007774:Lactation; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D008431:Maternal-Fetal Exchange; D009929:Organ Size / Q000187:drug effects; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D011467:Prostate / Q000187:drug effects / Q000254:growth & development; D017208:Rats, Wistar; D012741:Sexual Maturation / Q000187:drug effects*; D013076:Sperm Count; D013094:Spermatozoa / Q000002:abnormalities / Q000187:drug effects; D013737:Testis / Q000187:drug effects / Q000254:growth & development / Q000473:pathology; D013739:Testosterone / Q000097:blood
chemical_list: D000927:Anticonvulsants; D002220:Carbamazepine; D013739:Testosterone; D004958:Estradiol; D007986:Luteinizing Hormone
keywords: Breastfeeding; Carbamazepine; Leydig cell; Pregnancy; Puberty; Rat; Sperm parameters; Testosterone