title: [Effects of parthenolide on high glucose-induced cell proliferation, NF-κB activation and MCP-1 expression in rat mesangial cells].
abstract: OBJECTIVE
  To explore the effects of parthenolide (PTL) on high glucose-stimulated cell proliferation, NF-κB activation and monocyte chemoattractant protein-1 (MCP-1) expression in rat mesangial cells (MCs).
  
  
  METHODS
  MCs were cultured in normal glucose (5.6 mmol/L), high glucose (30 mmol/L), or high glucose with PTL. MTT assay was used to detect the cell proliferation. MCP-1 content in the supernatant was measured by ELISA, and the level of IκBα was detected by Western blotting to reflect NF-κB activity. EMSA method was used to measure the activation of NF-κB.
  
  
  RESULTS
  MC proliferation, MCP-1 expression and NF-κB activation were significantly enhanced and the expression of NF-κB-binding protein IκBα was obviously reduced in cells cultured in high glucose. Application of PTL obviously abolished the effects of high glucose.
  
  
  CONCLUSIONS
  PTL can suppress high glucose-stimulated NF-κB activation and MCP-1 expression in rat MC. These data provide a theoretical basis for the clinical application of PTL in prevention and control of diabetic nephropathy.
authors.affiliation: Department of Emergency, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. E-mail: xzz1008@163.com.
authors.forename: Zhaozhong
authors.initials: Z
authors.lastname: Xu
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Jia
authors.forename: Hongyu
authors.initials: H
authors.lastname: Li
authors.forename: Jiancheng
authors.initials: J
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Sijia
authors.initials: S
authors.lastname: Chen
authors.forename: Haibo
authors.initials: H
authors.lastname: Long
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology; D019659:Asteraceae / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology; D020672:I-kappa B Proteins / Q000378:metabolism; D050527:Mesangial Cells / Q000166:cytology* / Q000378:metabolism; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D020672:I-kappa B Proteins; D016328:NF-kappa B; C000606206:Nfkbia protein, rat; D012717:Sesquiterpenes; D000072000:NF-KappaB Inhibitor alpha; C002669:parthenolide; D005947:Glucose