title: Rapid and high-throughput N-glycomic analysis of plant glycoproteins.
abstract: Glycoprotein is a major element in higher organisms including mammalians and plants. It is widely accepted that variation in cellular N-glycome is related to modulation in dynamic cellular mechanisms such as cell-cell adhesion, cell activation, and malignant alterations in mammalian cells. However, the physiological importance of glycan modification of glycoproteins in plant cells is still a matter of dispute. Therefore, a comprehensive and high-throughput analysis of N-glycome in plant glycoproteins is needed. Here, an application of the glycoblotting-mass spectrometry technique to plant glycoprotein research is described.
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University, Niigata, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kaneko
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shiraya
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Mitsui
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Nishimura
mesh_terms: D002852:Chromatography, Ion Exchange; D054794:Glycomics / Q000379:methods*; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry; D057166:High-Throughput Screening Assays / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D013058:Mass Spectrometry; D012275:Oryza / Q000201:enzymology; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D013194:Staining and Labeling; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D010940:Plant Proteins; D011134:Polysaccharides; D000516:alpha-Amylases