title: [Changes of fecal flora and its correlation with inflammatory indicators in patients with inflammatory bowel disease].
abstract: OBJECTIVE
  To investigate the changes in fecal flora and its correlation with the occurrence and progression of inflammatory bowel disease (IBD).
  
  
  METHODS
  We collected fresh fecal specimens from 167 IBD patients (including 113 with ulcerative colitis and 54 with Crohn's disease) and 54 healthy volunteers. The fecal flora was analyzed by gradient dilution method and the data of inflammatory markers including WBC, PLT, CRP and ESR were collected to assess the association between the fecal flora and the inflammatory markers.
  
  
  RESULTS
  The species Enterrococcus (6.60∓0.23, P<0.01), Saccharomyces (2.22∓0.27, P<0.05), Bacteriodes (5.57∓0.28, P<0.001), Bifidobacterium (5.08∓0.30, P<0.01), Peptococcus (6.22∓0.25, P<0.001), Lactobacillus (6.00∓0.26, P<0.001), and Clostridium (3.57∓0.30, P<0.05) all increased significantly, while Eubacterium (1.56∓0.24, P<0.01) reduced markedly in patients with ulcerative colitis compared with those in the control subjects. Enterrococcus (6.93∓0.28, P<0.01), Saccharomyces (2.73∓0.37, P<0.01), Bacteriodes (4.32∓0.52, P<0.05), Bifidobacterium (4.88∓0.42, P<0.05), Peptococcus (6.19∓0.32, P<0.01) and Lactobacillus (4.73∓0.47, P<0.001) all increased significantly and Eubacterium (1.01∓0.29, P<0.01) and Clostridium (0.87∓0.31, P<0.01) decreased in patients with Crohn's disease. The positivity rates of bacterial culture were consistent with the results of quantitative analysis of the fecal flora. The changes in fecal flora did not show a significant correlation with these inflammatory markers.
  
  
  CONCLUSIONS
  IBD patients have fecal flora imbalance compared with the healthy controls, and this imbalance may contribute to the occurrence and progression of IBD. The decline of Eubacterium contributes to the occurrence and development of IBD.
authors.affiliation: Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangdong Provincial Key Laboratory of Gastroenterology, Guangzhou 510515, China. E-mail: zhangtingyikeda@126.com.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.forename: Ye
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhongqiu
authors.initials: Z
authors.lastname: Wang
authors.forename: Youlian
authors.initials: Y
authors.lastname: Zhou
authors.forename: Shaoheng
authors.initials: S
authors.lastname: Zhang
authors.forename: Pu
authors.initials: P
authors.lastname: Wang
authors.forename: Shan
authors.initials: S
authors.lastname: Xie
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
mesh_terms: D000328:Adult; D001419:Bacteria / Q000302:isolation & purification*; D001439:Bacteroides / Q000302:isolation & purification; D001644:Bifidobacterium / Q000302:isolation & purification; D015415:Biomarkers / Q000032:analysis; D003013:Clostridium / Q000302:isolation & purification; D003093:Colitis, Ulcerative / Q000382:microbiology; D003424:Crohn Disease / Q000382:microbiology; D016983:Enterococcus / Q000302:isolation & purification; D005051:Eubacterium / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000209:etiology / Q000382:microbiology*; D007778:Lactobacillus / Q000302:isolation & purification; D008297:Male; D010460:Peptococcus / Q000302:isolation & purification; D012440:Saccharomyces / Q000302:isolation & purification
chemical_list: D015415:Biomarkers