title: Chemical analysis and antioxidant activity of polysaccharides extracted from Inonotus obliquus sclerotia.
abstract: Three water-soluble polysaccharide fractions (IOP40, IOP60 and IOP80) were isolated by using different concentrations of alcohol precipitation from Inonotus obliquus sclerotia. Their physicochemical properties, including total sugar content, protein content, monosaccharide composition and percentage were analyzed. And their in vitro antioxidant capacities were investigated in terms of reducing power assay and scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals, hydroxyl radicals, superoxide anion radicals and hydrogen peroxide (H2O2). In general, three polysaccharide fractions exhibited increasing antioxidant activity with increasing concentration at the ranges of tested dosage. The orders of reducing power, DPPH-scavenging capacity, H2O2-scavenging capacity, and hydroxyl-scavenging activity were all IOP60>IOP40>IOP80. These findings demonstrated that three polysaccharide fractions extracted from I. obliquus, especially IOP60, could be employed as natural ingredients in functional food and pharmaceutical industry to alleviate the oxidative stress.
authors.affiliation: College of Life Science, Liaocheng University, Liaocheng, Shandong 252059, PR China. Electronic address: wyxx2008@163.com.
authors.forename: XiuJu
authors.initials: X
authors.lastname: Du
authors.forename: HongMei
authors.initials: H
authors.lastname: Mu
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhou
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: XiaoLi
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors; D059625:Liquid-Liquid Extraction / Q000379:methods
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D062610:Fungal Polysaccharides; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide
keywords: Antioxidant activity; Free radicals scavenging activity; Inonotus obliquus polysaccharide; Monosaccharide composition