title: Characterization of lung cancer by amide proton transfer (APT) imaging: an in-vivo study in an orthotopic mouse model.
abstract: Amide proton transfer (APT) imaging is one of the chemical exchange saturation transfer (CEST) imaging methods which images the exchange between protons of free tissue water and the amide groups (-NH) of endogenous mobile proteins and peptides. Previous work suggested the ability of APT imaging for characterization of the tumoral grade in the brain tumor. In this study, we tested the feasibility of in-vivo APT imaging of lung tumor and investigated whether the method could differentiate the tumoral types on orthotopic tumor xenografts from two malignant lung cancer cell lines. The results revealed that APT imaging is feasible to quantify lung tumors in the moving lung. The measured APT effect was higher in the tumor which exhibited more active proliferation than the other. The present study demonstrates that APT imaging has the potential to provide a characterization test to differentiate types or grade of lung cancer noninvasively, which may eventually reduce the need invasive needle biopsy or resection for lung cancer.
authors.affiliation: Advanced Imaging Research Center, Harold C. Simmons Comprehensive Cancer Center, UT Southwestern Medical Center, Dallas, Texas, United States of America.
authors.forename: Osamu
authors.initials: O
authors.lastname: Togao
authors.forename: Chase W
authors.initials: CW
authors.lastname: Kessinger
authors.forename: Gang
authors.initials: G
authors.lastname: Huang
authors.forename: Todd C
authors.initials: TC
authors.lastname: Soesbe
authors.forename: Koji
authors.initials: K
authors.lastname: Sagiyama
authors.forename: Ivan
authors.initials: I
authors.lastname: Dimitrov
authors.forename: A Dean
authors.initials: AD
authors.lastname: Sherry
authors.forename: Jinming
authors.initials: J
authors.lastname: Gao
authors.forename: Masaya
authors.initials: M
authors.lastname: Takahashi
mesh_terms: D000577:Amides / Q000737:chemistry*; D000818:Animals; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000473:pathology*; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D011522:Protons; D055425:Respiratory-Gated Imaging Techniques; D014867:Water / Q000737:chemistry*
chemical_list: D000577:Amides; D011522:Protons; D014867:Water