title: Cloning and sequence analysis of Peromyscus yucatanicus (Rodentia) Th1 (IL-12p35, IFN-γ and TNF) and Th2 (IL-4, IL-10 and TGF-β) cytokines.
abstract: The Yucatan deer mouse, Peromyscus yucatanicus (order Rodentia), is the principal reservoir of Leishmania (Leishmania) mexicana in the Yucatan peninsula of Mexico. Experimental infection results in clinical and histopathological features similar to those observed in humans with cutaneous leishmaniasis (CL) as well as peritoneal macrophage production of nitric oxide. These results support the possible use of P. yucatanicus as a novel experimental model to study CL caused by L. (L.) mexicana. However, immunological studies in these rodents have been limited by the lack of specific reagents. To address this issue, we cloned and analyzed cytokine sequences of P. yucatanicus as part of an effort to develop this species as a CL model. We cloned P. yucatanicus interleukin 4 (IL-4), IL-10, IL-12p35, gamma interferon, transforming growth factor beta and tumor necrosis factor partial cDNAs. Most of the P. yucatanicus sequences were highly conserved with orthologs of other mammalian species and the identity of all sequences were confirmed by the presence of conserved amino acids with possible biological functions in each putative polypeptide. The availability of these sequences is a first step which will allow us to carry out studies characterizing the immune response during pathogenic and nonpathogenic L. (L.) mexicana infections in P. yucatanicus.
authors.affiliation: Universidad Autonoma de Yucatan, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Laboratorio de Inmunologia, Ave. Itzaes No. 490 x 59-A, Merida, Yucatan, Mexico. Electronic address: nalleli.cervera@uady.mx.
authors.forename: Elsy Nalleli
authors.initials: EN
authors.lastname: Loria-Cervera
authors.forename: Erika Ivett
authors.initials: EI
authors.lastname: Sosa-Bibiano
authors.forename: Liliana Estefania
authors.initials: LE
authors.lastname: Villanueva-Lizama
authors.forename: Nicole Raymonde
authors.initials: NR
authors.lastname: Van Wynsberghe
authors.forename: Tony
authors.initials: T
authors.lastname: Schountz
authors.forename: Fernando Jose
authors.initials: FJ
authors.lastname: Andrade-Narvaez
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D007371:Interferon-gamma / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics; D053765:Interleukin-12 Subunit p35 / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D007894:Leishmania mexicana / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology*; D008297:Male; D008969:Molecular Sequence Data; D010542:Peromyscus; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D053765:Interleukin-12 Subunit p35; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma
keywords: CL; FBS; IFN-γ; IL; Leishmania; NCBI; NO; National Center for Biotechnology Information; Peromyscus yucatanicus; TGF-β; TNF; Th1/Th2 cytokines; cDNA; complementary DNA, RT-PCR reverse transcription polymerase chain reaction; cutaneous leishmaniasis; fetal bovine serum; iNOS; inducible nitric oxide synthase; interferon gamma; interleukin; mRNA; messenger RNA; nitric oxide; nt; nucleotide; transforming growth factor beta; tumor necrosis factor