title: Interactions of fish gelatin and chitosan in uncrosslinked and crosslinked with EDC films: FT-IR study.
abstract: Films based on fish gelatin, chitosan and blend of fish gelatin and chitosan before and after cross-linking with EDC have been characterized by FT-IR spectroscopy. The FT-IR spectrum of fish gelatin film showed the characteristic amide I, amide II and amide III bands, and the FT-IR spectrum of chitosan film confirmed that the polymer was only a partially deacetylated product, and included CH3-C=O and NH2 groups, the latter both in their free -NH2 and protonated -NH3(+) form. Analysis of FT-IR spectra of two-component, fish gelatin-chitosan film revealed the formation not only of hydrogen bonds within and between chains of polymers, but also of electrostatic interactions between -COO(-) of gelatin and -NH3(+) of chitosan. Modification with EDC provided cross-linking of composites of the film. New iso-peptide bonds formed between activated carboxylic acid groups of glutamic or aspartic acid residue of gelatin and amine groups of gelatin or/and chitosan.
authors.affiliation: Department of Food Chemistry, Technology and Biotechnology, Gdansk University of Technology, G. Narutowicza 11/12, 80-233 Gdańsk, Poland. Electronic address: hanna.staroszczyk@pg.gda.pl.
authors.forename: Hanna
authors.initials: H
authors.lastname: Staroszczyk
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Sztuka
authors.forename: Julia
authors.initials: J
authors.lastname: Wolska
authors.forename: Anna
authors.initials: A
authors.lastname: Wojtasz-Pająk
authors.forename: Ilona
authors.initials: I
authors.lastname: Kołodziejska
mesh_terms: D000818:Animals; D002234:Carbodiimides / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004123:Dimethylamines / Q000737:chemistry*; D005399:Fishes; D005780:Gelatin / Q000737:chemistry* / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D002234:Carbodiimides; D003432:Cross-Linking Reagents; D004123:Dimethylamines; C569266:N-ethyl-N'-(3-(dimethylamino)-propyl)carbodiimide; D005780:Gelatin; D048271:Chitosan
keywords: Coatings; FT-IR; Polymers; Thin films