title: Anti-lymphangiogenesis effects of a specific anti-interleukin 7 receptor antibody in lung cancer model in vivo.
abstract: Interleukin 7 (IL-7) is known to promote lymphangiogenesis. To study the relationship between IL-7 and the lymphangiogenesis in lung cancer cells xenograft tumors, we investigated how IL-7 regulates lymphangiogenesis by Quantitative real-time reverse transcriptase-polymerase chain reaction, Western blot, co-immunoprecipitation, chromatin immunoprecipitation, and immunohistochemistry methods. We found that, in lung cancer cells xenograft tumors IL-7/IL-7 receptor (IL-7R) increase the expression of VEGF-D and lymphangiogenesis, induce c-Fos and c-Jun heterodimer formation, and enhance c-Fos/c-Jun DNA binding activity to regulate VEGF-D. Taken together, our results provided evidence that IL-7/IL-7R induce VEGF-D upregulation and promote lymphangiogenesis via c-Fos/c-Jun pathway in lung cancer.
authors.affiliation: No. 202 Hospital of People Liberation Army of China, Shenyang, P.R., China.
authors.forename: Ming
authors.initials: M
authors.lastname: Jian
authors.forename: Zhang
authors.initials: Z
authors.lastname: Qingfu
authors.forename: Jiang
authors.initials: J
authors.lastname: Yanduo
authors.forename: Jiang
authors.initials: J
authors.lastname: Guocheng
authors.forename: Qiu
authors.initials: Q
authors.lastname: Xueshan
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D015851:Interleukin-7 / Q000378:metabolism*; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D042583:Lymphangiogenesis / Q000187:drug effects; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D020395:Receptors, Interleukin-7 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D042643:Vascular Endothelial Growth Factor D / Q000235:genetics* / Q000378:metabolism
chemical_list: D000906:Antibodies; D015851:Interleukin-7; D020395:Receptors, Interleukin-7; D042643:Vascular Endothelial Growth Factor D
keywords: in vivo; interleukin 7; interleukin 7 receptor; lung cancer; lymphangiogenesis