title: Fontibacillus phaseoli sp. nov. isolated from Phaseolus  vulgaris nodules.
abstract: A bacterial strain, designated BAPVE7BT, was isolated from root nodules of Phaseolus vulgaris in Spain. Phylogenetic analysis based on its 16S rRNA gene sequence placed the isolate into the genus Fontibacillus with Fontibacillus panacisegetis KCTC 13564T its closest relative with 97.1 % identity. The isolate was observed to be a Gram-positive, motile and sporulating rod. The catalase test was negative and oxidase was weak. The strain was found to reduce nitrate to nitrite and to produce β-galactosidase but the production of gelatinase, caseinase, urease, arginine dehydrolase, ornithine or lysine decarboxylase was negative. Acetoin production and aesculin hydrolysis were found to be positive. Growth was observed to be supported by many carbohydrates and organic acids as carbon source. MK-7 was identified as the predominant menaquinone and the major fatty acid (43.7 %) as anteiso-C15:0, as occurs in the other species of the genus Fontibacillus. Strain BAPVE7BT displayed a complex lipid profile consisting of diphosphatidylglycerol, phosphatidylglycerol, four glycolipids, four phospholipids, two lipids, two aminolipids and an aminophospholipid. Mesodiaminopimelic acid was detected in the peptidoglycan. The G+C content was determined to be 45.6 mol% (Tm). Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain BAPVE7BT should be considered a new species of genus Fontibacillus, for which the name Fontibacillus phaseoli sp. nov. is proposed (type strain, LMG 27589T, CECT 8333T).
authors.forename: José David
authors.initials: JD
authors.lastname: Flores-Félix
authors.forename: Rebeca
authors.initials: R
authors.lastname: Mulas
authors.forename: Martha-Helena
authors.initials: MH
authors.lastname: Ramírez-Bahena
authors.forename: María José
authors.initials: MJ
authors.lastname: Cuesta
authors.forename: Raúl
authors.initials: R
authors.lastname: Rivas
authors.forename: Javier
authors.initials: J
authors.lastname: Brañas
authors.forename: Daniel
authors.initials: D
authors.lastname: Mulas
authors.forename: Fernando
authors.initials: F
authors.lastname: González-Andrés
authors.forename: Alvaro
authors.initials: A
authors.lastname: Peix
authors.forename: Encarna
authors.initials: E
authors.lastname: Velázquez
mesh_terms: D056449:Bacillales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D027805:Phaseolus / Q000382:microbiology*; D010802:Phylogeny; D053204:Root Nodules, Plant / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D005227:Fatty Acids