title: Tissue distribution of hepatopancreatic parvo-like virus of shrimp in freshwater rice-field crab, Paratelphusa hydrodomous (Herbst).
abstract: An attempt was made to determine the replication efficiency of hepatopancreatic parvo-like virus (HPV) of shrimp in different organs of freshwater rice-field crab Paratelphusa hydrodomous (Herbst) using bioassay, PCR, RT-PCR, ELISA, Western blot and q-PCR analyses. Another attempt was made to use this crab as an alternative to penaeid shrimp for the large-scale production of HPV. This crab was found to be highly susceptible to HPV by intramuscular injection. The systemic HPV infection was confirmed by PCR and Western blot analyses in freshwater crab. The expression of capsid protein gene in different organs of infected crab was revealed by RT-PCR analysis. Indirect ELISA was used to quantify the capsid protein in different organs of the crab. The copy number of HPV in different organs of the infected crab was quantified by q-PCR. The results revealed a steady decrease in CT values in different organs of the infected crab during the course of infection. The viral inoculum that was prepared from different organs of the infected crab caused significant mortality in post-larvae of tiger prawn, Penaeus monodon (Fabricius). The results revealed that this rice-field crab could be used as an alternative host for HPV replication and also for large-scale production of HPV.
authors.affiliation: OIE Reference Laboratory for WTD, Aquaculture Biotechnology Laboratory, PG & Research Department of Zoology, C. Abdul Hakeem College, Vellore, India.
authors.forename: N
authors.initials: N
authors.lastname: Madan
authors.forename: T
authors.initials: T
authors.lastname: Rajkumar
authors.forename: N
authors.initials: N
authors.lastname: Sundar Raj
authors.forename: M A
authors.initials: MA
authors.lastname: Farook
authors.forename: K S N
authors.initials: KS
authors.lastname: Nambi
authors.forename: S
authors.initials: S
authors.lastname: Abdul Majeed
authors.forename: A S
authors.initials: AS
authors.lastname: Sahul Hameed
mesh_terms: D000818:Animals; D003386:Brachyura / Q000821:virology*; D036022:Capsid Proteins / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation; D012275:Oryza; D010321:Parvoviridae / Q000502:physiology*; D014018:Tissue Distribution; D014779:Virus Replication
chemical_list: D036022:Capsid Proteins
keywords: ELISA; PCR; Paratelphusa hydrodomous; Western blot; hepatopancreatic parvo-like virus