title: FABP3 and brown adipocyte-characteristic mitochondrial fatty acid oxidation enzymes are induced in beige cells in a different pathway from UCP1.
abstract: Cold exposure and β3-adrenergic receptor agonist (CL316,243) treatment induce the production of beige cells, which express brown adipocytes(BA)-specific UCP1 protein, in white adipose tissue (WAT). It remains unclear whether the beige cells, which have different gene expression patterns from BA, express BA-characteristic fatty acid oxidation (FAO) proteins. Here we found that 5 day cold exposure and CL316,243 treatment of WAT, but not CL316,243 treatment of primary adipocytes of C57BL/6J mice, increased mRNA levels of BA-characteristic FAO proteins. These results suggest that BA-characteristic FAO proteins are induced in beige cells in a different pathway from UCP1.
authors.affiliation: Department of Molecular Genetics, Institute of Life Science, Kurume University, 1-1 Hyakunen-kouen, Kurume 839-0864, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Takahiro
authors.initials: T
authors.lastname: Sato
authors.forename: Yuki
authors.initials: Y
authors.lastname: Shiimura
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Miura
authors.forename: Masayasu
authors.initials: M
authors.lastname: Kojima
mesh_terms: D052437:Adipocytes, Brown / Q000378:metabolism*; D052436:Adipose Tissue, White / Q000166:cytology*; D058667:Adrenergic beta-3 Receptor Agonists / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D003080:Cold Temperature; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D004149:Dioxoles / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D007473:Ion Channels / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D000071256:Uncoupling Protein 1
chemical_list: D058667:Adrenergic beta-3 Receptor Agonists; D004149:Dioxoles; C493409:Fabp3 protein, mouse; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604372:Ucp1 protein, mouse; D000071256:Uncoupling Protein 1; C076126:disodium (R,R)-5-(2-((2-(3-chlorophenyl)-2-hydroxyethyl)-amino)propyl)-1,3-benzodioxole-2,3-dicarboxylate; D005576:Colforsin; D000242:Cyclic AMP
keywords: 3-oxoacyl-Coenzyme A thiolase; ACAA2; ACADL; ACADM; ACADS; ACSL5; ACSS1; Acyl-CoA; BA; BAT; Beige cell/brite cell/recruitable brown fat cell; Brown adipocyte; CL; CL316,243; CPT1b; FABP3; FAO; Fatty acid oxidation; Heart-type fatty acid binding protein/fatty acid binding protein 3; PGC-1; PPAR; PPARγ coactivator 1; Rps18; SV; UCP1; WA; WAT; White adipocyte; acyl-CoA synthetase long-chain family member 5; acyl-CoA synthetase short-chain family member 1; acyl-Coenzyme A; brown adipocyte; brown adipose tissue; cAMP; cDNA; carnitine palmitoyltransferase 1b; complementary DNA; cyclic AMP; fatty acid binding protein3/heart-type fatty acid binding protein; fatty acid oxidation; i.p; intraperitoneal; long-chain acyl-Coenzyme A dehydrogenase; mRNA; medium-chain acyl-Coenzyme A dehydrogenase; messenger RNA; peroxisome proliferator-activated receptor; ribosomal protein S18; short-chain acyl-Coenzyme A dehydrogenase; stromal-vascular; subWAT; subcutaneous WAT; uncoupling protein 1; white adipocyte; white adipose tissue; β(3)-adrenergic receptor; β(3)AR