title: The cohesin complex regulates immunoglobulin class switch recombination.
abstract: Immunoglobulin (Ig) class switch recombination (CSR) is initiated by the transcription-coupled recruitment of activation-induced cytidine deaminase (AID) to switch regions and by the subsequent generation of double-stranded DNA breaks (DSBs). These DNA breaks are ultimately resolved through the nonhomologous end joining (NHEJ) pathway. We show that during CSR, AID associates with subunits of cohesin, a complex previously implicated in sister chromatid cohesion, DNA repair, and the formation of DNA loops between enhancers and promoters. Furthermore, we implicate the cohesin complex in the mechanism of CSR by showing that cohesin is dynamically recruited to the Sμ-Cμ region of the IgH locus during CSR and that knockdown of cohesin or its regulatory subunits results in impaired CSR and increased usage of microhomology-based end joining.
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Institut National de la Santé et de la Recherche Médicale (INSERM) U964/Centre National de la Recherche Scientifique (CNRS) UMR 7104/Université de Strasbourg, 67404 Illkirch, France.
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Thomas-Claudepierre
authors.forename: Ebe
authors.initials: E
authors.lastname: Schiavo
authors.forename: Vincent
authors.initials: V
authors.lastname: Heyer
authors.forename: Marjorie
authors.initials: M
authors.lastname: Fournier
authors.forename: Adeline
authors.initials: A
authors.lastname: Page
authors.forename: Isabelle
authors.initials: I
authors.lastname: Robert
authors.forename: Bernardo
authors.initials: B
authors.lastname: Reina-San-Martin
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D003564:Cytidine Deaminase / Q000378:metabolism; D059766:DNA End-Joining Repair; D055785:Gene Knockdown Techniques; D017578:Immunoglobulin Class Switching; D051379:Mice; D011995:Recombination, Genetic; D000097722:Cohesins; D000098550:Structural Maintenance of Chromosome Protein 1; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
chemical_list: D018797:Cell Cycle Proteins; D011508:Chondroitin Sulfate Proteoglycans; D002868:Chromosomal Proteins, Non-Histone; D003564:Cytidine Deaminase; D000097722:Cohesins; D000098550:Structural Maintenance of Chromosome Protein 1; D000098830:AICDA (Activation-Induced Cytidine Deaminase); C492317:SMC3 protein, mouse