title: The influence of Selol on the expression of oxidative stress genes in normal and malignant prostate cells.
abstract: Selol is a mixture of selenitriglycerides, obtained by the chemical modification of sunflower oil, which contain selenium at the +4 oxidation state. The aim of the present study was to describe the changes in the expression of genes related to oxidative stress caused by Selol in prostate cells: both normal (PNT1A) and malignant (LNCaP). The changes in gene expression in PNT1A and LNCaP cell lines under the influence of Selol were measured using a 96-well RT(2) Profiler ™PCR Array: Human Oxidative Stress and Antioxidant Defense, which arrayed 84 genes functionally involved in the cellular oxidative stress response. Based on the obtained data, LNCaP cells exhibited a significantly lower potential for antioxidant defence when compared to PNT1A cells. The response of the malignant LNCaP cells to exposure to Selol was significantly different from that of the normal PNT1A cells, especially after 48 h of incubation. In the case of LNCaP cells, Selol causes down-regulation of the expression of many vital genes. Under in vitro conditions, the efficacy of Selol slightly changes with increasing concentration, but significantly increases when the incubation time is lengthened.
authors.affiliation: Department of Biochemistry and Biofarmaceuticals, National Medicines Institue, Chelmska 30/34, 00-725 Warsaw, Poland. i.ksiazek@nil.gov.pl.
authors.forename: Iza
authors.initials: I
authors.lastname: Ksiazek
authors.forename: Karolina
authors.initials: K
authors.lastname: Sitarz
authors.forename: Magdalena
authors.initials: M
authors.lastname: Roslon
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Anuszewska
authors.forename: Piotr
authors.initials: P
authors.lastname: Suchocki
authors.forename: Jadwiga Dudkiewicz
authors.initials: JD
authors.lastname: Wilczynska
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D015536:Down-Regulation; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics*; D011467:Prostate / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D018036:Selenium Compounds / Q000494:pharmacology*
chemical_list: D018036:Selenium Compounds; C105370:selol
keywords: LNCaP; PNT1A; Selol; gene expression; oxidative stress