title: Determination of hinokitiol in skin lotion by high-performance liquid chromatography-ultraviolet detection after precolumn derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole.
abstract: Hinokitiol, a potent, broad-spectrum antibacterial agent, is a component of various personal care products. In this study, the concentration of hinokitiol in skin lotion was analyzed by means of high-performance liquid chromatography-ultraviolet detection (380 nm) after precolumn derivatization with 4-fluoro-7-nitro-2,1, 3-benzoxadiazole (NBD-F). A standard curve was obtained after derivatization of the authentic compound with NBD-F in borate buffer (pH 9.0) at 60°C for 10 min. The retention time of NBD-hinokitiol was 7.2 min. The calibration plot was linear in the range of 0.2 to 4 mg/ml with an r2 value of 0.9985, and the lower limit of detection was 0.05 μg/ml (at a signal-to-noise ratio of 3, absolute amount of 0.33 ng/20 μl injection). The coefficient of variation was less than 9.4%. It was found that the amount of hinokitiol in the tested skin lotion was 194 ± 14 μg/ml (range: 180-212 μg/ml). Recovery in addition-recovery tests was within the range of 84.5% to 98.0%. This system is simple, sensitive, and convenient, and should be suitable for routine quality assessment of personal care products containing hinokitiol.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa 920-1181, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Higashi
authors.forename: Youichi
authors.initials: Y
authors.lastname: Fujii
mesh_terms: D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives* / Q000737:chemistry; D000890:Anti-Infective Agents / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D057230:Limit of Detection; D039821:Monoterpenes / Q000032:analysis*; D059629:Signal-To-Noise Ratio; D063465:Skin Cream / Q000737:chemistry*; D014334:Tropolone / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D000890:Anti-Infective Agents; D039821:Monoterpenes; C032930:7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole; D014334:Tropolone; D009327:4-Chloro-7-nitrobenzofurazan; C009479:beta-thujaplicin