title: High-resolution X-ray crystal structure of bovine H-protein using the high-pressure cryocooling method.
abstract: Recently, many technical improvements in macromolecular X-ray crystallography have increased the number of structures deposited in the Protein Data Bank and improved the resolution limit of protein structures. Almost all high-resolution structures have been determined using a synchrotron radiation source in conjunction with cryocooling techniques, which are required in order to minimize radiation damage. However, optimization of cryoprotectant conditions is a time-consuming and difficult step. To overcome this problem, the high-pressure cryocooling method was developed (Kim et al., 2005) and successfully applied to many protein-structure analyses. In this report, using the high-pressure cryocooling method, the X-ray crystal structure of bovine H-protein was determined at 0.86 Å resolution. Structural comparisons between high- and ambient-pressure cryocooled crystals at ultra-high resolution illustrate the versatility of this technique. This is the first ultra-high-resolution X-ray structure obtained using the high-pressure cryocooling method.
authors.affiliation: Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Akifumi
authors.initials: A
authors.lastname: Higashiura
authors.forename: Kazunori
authors.initials: K
authors.lastname: Ohta
authors.forename: Mika
authors.initials: M
authors.lastname: Masaki
authors.forename: Masaru
authors.initials: M
authors.lastname: Sato
authors.forename: Koji
authors.initials: K
authors.lastname: Inaka
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Tanaka
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nakagawa
mesh_terms: D000818:Animals; D002417:Cattle; D003080:Cold Temperature; D018360:Crystallography, X-Ray / Q000379:methods*; D008958:Models, Molecular; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins
keywords: H-protein; high-pressure cryocooling method; high-resolution X-ray crystallography