title: Heterologous expression in budding yeast as a tool for studying the plant cell morphogenesis machinery.
abstract: The budding yeast (Saccharomyces cerevisiae) can serve as a unique experimental system for functional studies of heterologous genes, allowing not only complementation of readily available yeast mutations but also generation of overexpression phenotypes and in some cases also rescue of such phenotypes. Here we summarize the main considerations that have to be taken into account when using the yeast expression system for investigating the function of plant genes participating in cell morphogenesis; outline the strategies of experiment planning, yeast strain selection (or construction), and expression vector choice; and provide detailed protocols for yeast transformation, transformant selection, and phenotype evaluation.
authors.affiliation: Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic.
authors.forename: Fatima
authors.initials: F
authors.lastname: Cvrčková
authors.forename: Michal
authors.initials: M
authors.lastname: Hála
mesh_terms: D015870:Gene Expression; D015723:Gene Library; D017343:Genes, Plant; D005816:Genetic Complementation Test; D010641:Phenotype; D059828:Plant Cells / Q000378:metabolism*; D063245:Plant Development / Q000502:physiology*; D010957:Plasmids / Q000235:genetics; D004718:Saccharomycetales / Q000235:genetics*; D014170:Transformation, Genetic