title: [Research on HPLC fingerprint of Rheum palmatum from Gansu Province called "quan-shui-da-huang"].
abstract: OBJECTIVE
  To establish an analysis method for HPLC fingerprint of Rheum palmatum collected from Gansu province (called "Quan-shui-da-huang"), and provide basis for quality control of Quan-shui-da-huang.
  
  
  METHODS
  The Cosmosil 5C18-PAQ column (250 mm x 4.6 mm, 5 microm) was used with the mixture of 0.1% acetic acid and acetonitrile as mobile phase in a gradient elution mode. The flow rate was 1 mL/min, column temperature was 30 degrees C and detection wavelength was set at 280 nm. The similarity evaluation, principal component analysis (PCA) and cluster analysis of crude drugs collected from different habitats were carried out.
  
  
  RESULTS
  The analysis method of HPLC fingerprint was set up. The characteristic fingerprint was obtained by chemometrics methods.
  
  
  CONCLUSIONS
  Under the selected chromatographic conditions, constituents in Rheum palmatum can be separated well. This method can be used for the quality control of Quan-shui-da-huang.
authors.affiliation: Institute for Food and Cosmetics Control, National Institutes for Food and Drug Control, Beijing 100050, China.
authors.forename: Zheng-Rui
authors.initials: ZR
authors.lastname: Niu
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Liu
authors.forename: Qing-Sheng
authors.initials: QS
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: Cao
authors.forename: Shu-Jie
authors.initials: SJ
authors.lastname: Mao
authors.forename: Xian-Duan
authors.initials: XD
authors.lastname: Li
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000592:standards; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development; D025341:Principal Component Analysis; D011786:Quality Control; D012250:Rheum / Q000737:chemistry* / Q000254:growth & development
chemical_list: D004365:Drugs, Chinese Herbal