title: Differential effect of 14 free fatty acids in the expression of inflammation markers on human arterial coronary cells.
abstract: Cardiovascular disease is the leading cause of death in the United States, and circulating free fatty acids (FFAs) are known risk factors associated with cardiovascular inflammation. The influence of 14 dietary FFAs (including saturated, mono- and polyunsaturated, and trans) on the expression of inflammatory markers in human coronary arterial smooth muscle (HCASM) and endothelial (HCEC) cells using a cell culture model was investigated. HCASM and HCEC cell cultures were incubated with 200 μM of each FFA for 8 or 24 h, respectively, at 37 °C in a 5% CO2 humidified incubator. Inflammatory markers were assessed by ELISA or Western blot in the supernatant or cell lysates respectively. Results showed significant differences in the expression of inflammatory markers among the fatty acid treatments and the control, with myristic and palmitic acids being identified as the most and linoleic acid as the least pro-inflammatory. This suggests that FFAs may induce low-grade inflammation in human coronary arterial cells and provides more information on mode of action.
authors.affiliation: Department of Food Science, Louisiana State University Agricultural Center , Baton Rouge, Louisiana 70803, United States.
authors.forename: Adriana
authors.initials: A
authors.lastname: Soto-Vaca
authors.forename: Jack N
authors.initials: JN
authors.lastname: Losso
authors.forename: Karen
authors.initials: K
authors.lastname: McDonough
authors.forename: John W
authors.initials: JW
authors.lastname: Finley
mesh_terms: D015415:Biomarkers / Q000032:analysis; D002460:Cell Line; D018932:Chemokine CCL2 / Q000276:immunology; D003331:Coronary Vessels / Q000166:cytology / Q000276:immunology*; D042783:Endothelial Cells / Q000276:immunology; D005227:Fatty Acids / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D018836:Inflammation Mediators / Q000276:immunology*; D015850:Interleukin-6 / Q000276:immunology; D016209:Interleukin-8 / Q000276:immunology; D032389:Myocytes, Smooth Muscle / Q000276:immunology
chemical_list: D015415:Biomarkers; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D005227:Fatty Acids; D018836:Inflammation Mediators; D015850:Interleukin-6; D016209:Interleukin-8