title: Simultaneous identification and quantification of nitrosylation sites by combination of biotin switch and ICAT labeling.
abstract: S-nitrosylation is a widespread modification of proteins. In plants, most information available to date regarding this modification was obtained using nitric oxide donors and concerned the proteins but not the identification of cysteine residues specifically modified in the proteins or their quantification. Here, we describe a method for the identification of endogenously nitrosylated cysteines in Arabidopsis and, simultaneously, the measurement of relative change in their abundance within binary comparisons.
authors.affiliation: LPF UR1199 Campus SupAgro/INRA, Montpellier, France.
authors.forename: Abasse
authors.initials: A
authors.lastname: Fares
authors.forename: Claude
authors.initials: C
authors.lastname: Nespoulous
authors.forename: Michel
authors.initials: M
authors.lastname: Rossignol
authors.forename: Jean-Benoît
authors.initials: JB
authors.lastname: Peltier
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism*; D001710:Biotin / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods*; D013058:Mass Spectrometry; D015538:Nitrosation; D026403:S-Nitrosothiols / Q000378:metabolism; D013223:Statistics as Topic
chemical_list: D029681:Arabidopsis Proteins; D026403:S-Nitrosothiols; D001710:Biotin