title: Culturing of human nasal epithelial cells at the air liquid interface.
abstract: In vitro models using human primary epithelial cells are essential in understanding key functions of the respiratory epithelium in the context of microbial infections or inhaled agents. Direct comparisons of cells obtained from diseased populations allow us to characterize different phenotypes and dissect the underlying mechanisms mediating changes in epithelial cell function. Culturing epithelial cells from the human tracheobronchial region has been well documented, but is limited by the availability of human lung tissue or invasiveness associated with obtaining the bronchial brushes biopsies. Nasal epithelial cells are obtained through much less invasive superficial nasal scrape biopsies and subjects can be biopsied multiple times with no significant side effects. Additionally, the nose is the entry point to the respiratory system and therefore one of the first sites to be exposed to any kind of air-borne stressor, such as microbial agents, pollutants, or allergens. Briefly, nasal epithelial cells obtained from human volunteers are expanded on coated tissue culture plates, and then transferred onto cell culture inserts. Upon reaching confluency, cells continue to be cultured at the air-liquid interface (ALI), for several weeks, which creates more physiologically relevant conditions. The ALI culture condition uses defined media leading to a differentiated epithelium that exhibits morphological and functional characteristics similar to the human nasal epithelium, with both ciliated and mucus producing cells. Tissue culture inserts with differentiated nasal epithelial cells can be manipulated in a variety of ways depending on the research questions (treatment with pharmacological agents, transduction with lentiviral vectors, exposure to gases, or infection with microbial agents) and analyzed for numerous different endpoints ranging from cellular and molecular pathways, functional changes, morphology, etc. In vitro models of differentiated human nasal epithelial cells will enable investigators to address novel and important research questions by using organotypic experimental models that largely mimic the nasal epithelium in vivo.
authors.affiliation: Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill.
authors.forename: Loretta
authors.initials: L
authors.lastname: Müller
authors.forename: Luisa E
authors.initials: LE
authors.lastname: Brighton
authors.forename: Johnny L
authors.initials: JL
authors.lastname: Carson
authors.forename: William A
authors.initials: WA
authors.lastname: Fischer
authors.forename: Ilona
authors.initials: I
authors.lastname: Jaspers
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D003470:Culture Media; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D009297:Nasal Mucosa / Q000166:cytology*
chemical_list: D003470:Culture Media