title: Preparation and characterization of an advanced medical device for bone regeneration.
abstract: Tridimensional scaffolds can promote bone regeneration as a framework supporting the migration of cells from the surrounding tissue into the damaged tissue and as delivery systems for the controlled or prolonged release of cells, genes, and growth factors. The goal of the work was to obtain an advanced medical device for bone regeneration through coating a decellularized and deproteinized bone matrix of bovine origin with a biodegradable, biocompatible polymer, to improve the cell engraftment on the bone graft. The coating protocol was studied and set up to obtain a continuous and homogeneous polylactide-co-glycolide (PLGA) coating on the deproteinized bone matrix Orthoss® block without occluding pores and decreasing the scaffold porosity. The PLGA-coated scaffolds were characterized for their morphology and porosity. The effects of PLGA polymer coating on cell viability were assessed with the 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium assay. The polymer solution concentration and the number of polymeric layers were the main variables affecting coating efficiency and porosity of the original decellularized bone matrix. The designed polymer coating protocol did not affect the trabecular structure of the original decellularized bone matrix. The PLGA-coated decellularized bone matrix maintained the structural features, and it improved the ability in stimulating fibroblasts attachment and proliferation.
authors.affiliation: Department of Drug Sciences, University of Pavia, Via Taramelli 12-14, 27100, Pavia, Italy.
authors.forename: Rossella
authors.initials: R
authors.lastname: Dorati
authors.forename: Claudia
authors.initials: C
authors.lastname: Colonna
authors.forename: Ida
authors.initials: I
authors.lastname: Genta
authors.forename: Giovanna
authors.initials: G
authors.lastname: Bruni
authors.forename: Livia
authors.initials: L
authors.lastname: Visai
authors.forename: Bice
authors.initials: B
authors.lastname: Conti
mesh_terms: D020341:Absorbable Implants; D001857:Bone Matrix / Q000737:chemistry / Q000502:physiology; D001861:Bone Regeneration / Q000502:physiology*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004864:Equipment and Supplies; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid