title: Evaluation of a multitarget real-time PCR assay for detection of Bordetella species during a pertussis outbreak in New Hampshire in 2011.
abstract: A multitarget real-time PCR assay with three targets, including insertion sequence 481 (IS481), IS1001, and an IS1001-like element, as well as pertussis toxin subunit S1 (ptxS1), for the detection of Bordetella species was evaluated during a pertussis outbreak. The sensitivity and specificity were 77 and 88% (PCR) and 66 and 100% (culture), respectively. All patients with an IS481 C(T) of <30 also tested positive by ptxS1 assay and were clinical pertussis cases. No patients with IS481 C(T) values of ≥40 tested positive by culture. Therefore, we recommend that culture be performed only for specimens with IS481 C(T) values of 30 ≤ CT <40.
authors.affiliation: New Hampshire Department of Health and Human Services, Division of Public Health Services, Concord, New Hampshire, USA.
authors.forename: Fengxiang
authors.initials: F
authors.lastname: Gao
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Mahoney
authors.forename: Elizabeth R
authors.initials: ER
authors.lastname: Daly
authors.forename: Wendy
authors.initials: W
authors.lastname: Lamothe
authors.forename: Daniel
authors.initials: D
authors.lastname: Tullo
authors.forename: Christine
authors.initials: C
authors.lastname: Bean
mesh_terms: D000293:Adolescent; D001431:Bacteriological Techniques / Q000379:methods*; D001884:Bordetella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004196:Disease Outbreaks; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009513:New Hampshire / Q000453:epidemiology; D037342:Pertussis Toxin / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014917:Whooping Cough / Q000175:diagnosis* / Q000453:epidemiology* / Q000382:microbiology
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C083388:pertussis toxin, S1 subunit; D037342:Pertussis Toxin