title: A faster reconstitution of hematopoiesis after autologous transplantation of hematopoietic cells cryopreserved in 7.5% dimethyl sulfoxide if compared to 10% dimethyl sulfoxide containing medium.
abstract: Our previous in vitro studies proved a higher clonogenic potential of peripheral blood progenitor cells cryopreserved in 7.5% dimethyl sulfoxide (Me2SO) than in 10% Me2SO containing medium. Based on this findings 7.5% Me2SO cryopreservation medium was introduced to our protocol and both the hematopoietic recovery and infusion-related toxicity were compared with that obtained with standard 10% Me2SO containing solution. Two cohorts of consecutive patients treated with autologous hematopoietic stem cell transplantation were included in the analysis: 56 patients with PBPCs cryopreserved in 7.5% Me2SO solution and 52 patients who obtained cells cryopreserved in 10% Me2SO. Both study groups did not differ significantly with regard to age, diagnosis, and the number of transplanted CD34(+) cells. The time to leukocyte recovery was shorter for patients in the 7.5% Me2SO treated group than in the 10% one. Reconstitution of platelets and the frequency of adverse events did not differ in both groups. Reduction of Me2SO concentration from 10% to 7.5% in cryoprotective mixture has a beneficial impact on leukocyte recovery. These findings require verification in a prospective, randomized trial.
authors.affiliation: Department of Bone Marrow Transplantation and Oncohematology, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze Armii Krajowej 15, 44-101 Gliwice, Poland. Electronic address: mitrus@io.gliwice.pl.
authors.forename: Iwona
authors.initials: I
authors.lastname: Mitrus
authors.forename: Andrzej
authors.initials: A
authors.lastname: Smagur
authors.forename: Sebastian
authors.initials: S
authors.lastname: Giebel
authors.forename: Joanna
authors.initials: J
authors.lastname: Gliwinska
authors.forename: Magdalena
authors.initials: M
authors.lastname: Prokop
authors.forename: Magdalena
authors.initials: M
authors.lastname: Glowala-Kosinska
authors.forename: Agata
authors.initials: A
authors.lastname: Chwieduk
authors.forename: Maria
authors.initials: M
authors.lastname: Sadus-Wojciechowska
authors.forename: Andrzej
authors.initials: A
authors.lastname: Tukiendorf
authors.forename: Jerzy
authors.initials: J
authors.lastname: Holowiecki
mesh_terms: D000328:Adult; D000368:Aged; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000378:metabolism*; D005260:Female; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D014182:Transplantation, Autologous; D055815:Young Adult
chemical_list: D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide
keywords: Autologous PBPC transplantation; Cryopreservation; Hematopoietic recovery; Me(2)SO