title: MALDI-TOF MS: an upcoming tool for rapid detection of antibiotic resistance in microorganisms.
abstract: MALDI-TOF MS profiling for microorganism detection has already been demonstrated in the 1990s, but has evolved to the first-line identification method in many laboratories just during the past five years. While this application of MALDI-TOF MS has proven its broad applicability, accuracy, robustness, and cost-effectiveness it is of particular interest to expand the capabilities of the mass spectrometric platform. Resistance detection is the most desirable further application of MALDI-TOF MS in microbiology, but maybe also the most challenging. Different approaches have been published regarding diverse antibiotic drugs and distinct microorganism classes. The current review shall give an overview about the developments of the recent years and their potential to get transformed in clinical useful assays in the future.
authors.affiliation: Bruker Daltonik GmbH, Bremen, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Kostrzewa
authors.forename: Katrin
authors.initials: K
authors.lastname: Sparbier
authors.forename: Thomas
authors.initials: T
authors.lastname: Maier
authors.forename: Sören
authors.initials: S
authors.lastname: Schubert
mesh_terms: D004352:Drug Resistance, Microbial; D008828:Microbiological Techniques / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000592:standards / Q000639:trends*
keywords: Clinical microbiology; Diagnostic; MALDI-TOF MS; Resistance detection