title: Resistance to influenza A virus infection in transformed cell lines expressing an anti-PB2 monoclonal antibody.
abstract: The polymerase basic 2 (PB2) protein is one of four proteins that make up the influenza A virus replication complex, which is responsible for viral gene transcription and replication. To assess the antiviral potential of an anti-PB2 monoclonal antibody that inhibits RNA transcription of influenza A viruses, Mardin-Darby canine kidney (MDCK) cells were transformed with two transgenes that encode the light and heavy chains of the monoclonal antibody. The transformed cell lines expressing this monoclonal antibody displayed resistance to several subtypes of influenza A virus infection. In the transformed cell lines infected with influenza A virus, the level of viral RNA transcription was decreased and the effective nuclear transportation of the PB2 protein was also inhibited. These results demonstrate that the anti-PB2 intrabody is potentially able to interfere with the effective nuclear transportation of PB2 protein, resulting in the observed resistance to influenza A virus infection in vitro.
authors.affiliation: Department of Biomedicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan. Electronic address: yosikazu@anim.med.kyushu-u.ac.jp.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: Kinuyo
authors.initials: K
authors.lastname: Ozaki
authors.forename: Masahiro
authors.initials: M
authors.lastname: Maeda
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Nishijima
authors.forename: Hiroki
authors.initials: H
authors.lastname: Takakuwa
authors.forename: Koichi
authors.initials: K
authors.lastname: Otsuki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kida
authors.forename: Etsuro
authors.initials: E
authors.lastname: Ono
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000235:genetics* / Q000378:metabolism; D000998:Antiviral Agents / Q000378:metabolism; D002461:Cell Line, Transformed; D002642:Chick Embryo; D002645:Chickens; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D019085:Fluorescent Antibody Technique, Direct / Q000662:veterinary; D009980:Influenza A virus / Q000235:genetics* / Q000378:metabolism; D061985:Madin Darby Canine Kidney Cells; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D000914:Antibodies, Viral; D000998:Antiviral Agents; C057027:PB2 protein, Influenzavirus A; D012367:RNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase
keywords: Antiviral effect; Influenza A virus; Intrabody; PB2 protein; Viral RNA transcription