title: Scutellaria polysaccharide inhibits the infectivity of Newcastle disease virus to chicken embryo fibroblast.
abstract: BACKGROUND
  To select the antiviral active site of Scutellaria polysaccharide (SPS), safe concentrations of crude total Scutellaria polysaccharide (SPS(t)) and fractional polysaccharide SPS₅₀, SPS₆₀, SPS₇₀ and SPS₈₀ on chicken embryo fibroblast (CEF) were first compared using the MTT method. Then, SPS(t), SPS₅₀, SPS₆₀, SPS₇₀, and SPS₈₀ at five concentrations within the safe concentration, together with Newcastle disease virus (NDV), were added to the cultivating system of CEF in three models: pre-addition of polysaccharide, post-addition of polysaccharide, and simultaneous addition of polysaccharides and NDV after mixing. The effects of SPS on the cellular infectivity of NDV (A₅₇₀ value and the highest viral inhibitory rate) were compared using the MTT method.
  
  
  RESULTS
  At appropriate concentrations, the five polysaccharides could significantly inhibit the infectivity of NDV on CEF. Among the five polysaccharide groups, the SPS₈₀ group exhibited the highest viral inhibitory rate in the three sample-addition modes.
  
  
  CONCLUSIONS
  This finding indicates that SPS₈₀ possesses the best efficacy as a component of antiviral polysaccharide drug.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Xiaona
authors.forename: Liu
authors.initials: L
authors.lastname: Jianzhu
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000009:adverse effects / Q000302:isolation & purification / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D055808:Drug Discovery; D004365:Drugs, Chinese Herbal / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology; D020834:Ethnopharmacology; D005347:Fibroblasts / Q000166:cytology / Q000821:virology; D005590:Fractional Precipitation; D008516:Medicine, Chinese Traditional; D008826:Microbial Sensitivity Tests; D009522:Newcastle disease virus / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000009:adverse effects / Q000302:isolation & purification / Q000494:pharmacology*; D031363:Scutellaria / Q000737:chemistry*; D013047:Specific Pathogen-Free Organisms; D038301:Virus Inactivation / Q000187:drug effects; D018406:Virus Physiological Phenomena / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides