title: Temperature-sensitive elastin-mimetic dendrimers: Effect of peptide length and dendrimer generation to temperature sensitivity.
abstract: Dendrimers are synthetic macromolecules with unique structure, which are a potential scaffold for peptides. Elastin is one of the main components of extracellular matrix and a temperature-sensitive biomacromolecule. Previously, Val-Pro-Gly-Val-Gly peptides have been conjugated to a dendrimer for designing an elastin-mimetic dendrimer. In this study, various elastin-mimetic dendrimers using different length peptides and different dendrimer generations were synthesized to control the temperature dependency. The elastin-mimetic dendrimers formed β-turn structure by heating, which was similar to the elastin-like peptides. The elastin-mimetic dendrimers exhibited an inverse phase transition, largely depending on the peptide length and slightly depending on the dendrimer generation. The elastin-mimetic dendrimers formed aggregates after the phase transition. The endothermal peak was observed in elastin-mimetic dendrimers with long peptides, but not with short ones. The peptide length and the dendrimer generation are important factors to tune the temperature dependency on the elastin-mimetic dendrimer.
authors.affiliation: Nanoscience and Nanotechnology Research Center, Research Organization for the 21st Century, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8570, Japan.
authors.forename: Chie
authors.initials: C
authors.lastname: Kojima
authors.forename: Kotaro
authors.initials: K
authors.lastname: Irie
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tada
authors.forename: Naoki
authors.initials: N
authors.lastname: Tanaka
mesh_terms: D000595:Amino Acid Sequence; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D050091:Dendrimers / Q000138:chemical synthesis / Q000737:chemistry*; D004549:Elastin / Q000138:chemical synthesis / Q000737:chemistry* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D044367:Phase Transition; D017433:Protein Structure, Secondary; D013696:Temperature
chemical_list: D050091:Dendrimers; D010455:Peptides; D004549:Elastin
keywords: dendrimer; elastin; peptide; phase transition; temperature-sensitive