title: Contrasting of biological samples for X-ray synchrotron microtomography.
abstract: The method of contrasting with iodine ions was developed to obtain high-resolution 3D images of large biological specimens using a synchrotron X-ray microtomography unit. It was shown that the samples (late mouse embryos) treated with 50% Lugol solution with addition of 25% ethanol for 48 h followed by a 48-h washout in phosphate buffered saline had maximum contrast and lowest compression artifacts. Processing of samples by this protocol allowed detecting zones of active proliferation. Incubation of brain samples for 120 h in 7.6% meglumine/sodium diatrizoate without washout ensured the best contrast during myelin identification.
authors.affiliation: Laboratory of Neurobiology of Memory, P. K. Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences; Neurocognitive NTK, Kurchatov NBIC-center, National Research Centre "Kurchatov Institute"; Synchrotron and Neutron NTK, Kurchatov NBIC-center, National Research Centre "Kurchatov Institute", Moscow, Russia. Efimova_OI@nrcki.ru.
authors.forename: O I
authors.initials: OI
authors.lastname: Efimova
authors.forename: A S
authors.initials: AS
authors.lastname: Khlebnikov
authors.forename: R A
authors.initials: RA
authors.lastname: Senin
authors.forename: P A
authors.initials: PA
authors.lastname: Voronin
authors.forename: K V
authors.initials: KV
authors.lastname: Anokhin
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging; D003287:Contrast Media; D004622:Embryo, Mammalian / Q000000981:diagnostic imaging; D005260:Female; D007454:Iodides; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D055114:X-Ray Microtomography / Q000379:methods*
chemical_list: D003287:Contrast Media; D007454:Iodides; C010389:Lugol's solution