title: Comparing the effect of equiaxial cyclic mechanical stimulation on GATA4 expression in adipose-derived and bone marrow-derived mesenchymal stem cells.
abstract: Myocardium is prone to mechanical stimuli among which pulsatile blood flow exerts both radial and longitudinal strains on the heart. Recent studies have shown that mechanical stimulation can notably influence regeneration of cardiac muscle cells. GATA4 is a cardiac-specific transcription factor that plays an important role in late embryonic heart development. Our study aimed at investigating the effect of equiaxial cyclic strain on GATA4 expression in adipose-derived (ASCs) and bone marrow-derived (BMSCs) mesenchymal stem cells. For this reason, both ASCs and BMSCs were studied in four distinct groups of chemical, mechanical, mechano-chemical and negative control. According to this categorisation, the cells were exposed to cyclic mechanical loading and/or 5-azacytidine as the chemical factor. The level of GATA4 expression was then quantified using real-time PCR method on the first, fourth and seventh days. The results show that: (1) equiaxial cyclic stimulation of mesenchymal stem cells could promote GATA4 expression from the early days of induction and as it went on, its combination with chemical factor elevated expression; (2) cyclic strain could accelerate GATA4 expression compared to the chemical factor; (3) in this regard, these results indicate a higher capacity of ASCs than BMSCs to express GATA4.
authors.affiliation: Cardiovascular Lab, Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Susan
authors.initials: S
authors.lastname: Amin
authors.forename: Seyede Elnaz
authors.initials: SE
authors.lastname: Banijamali
authors.forename: Mohammad
authors.initials: M
authors.lastname: Tafazoli-Shadpour
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Shokrgozar
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Dehghan
authors.forename: Nooshin
authors.initials: N
authors.lastname: Haghighipour
authors.forename: Reza
authors.initials: R
authors.lastname: Mahdian
authors.forename: Vahid
authors.initials: V
authors.lastname: Bayati
authors.forename: Pegah
authors.initials: P
authors.lastname: Abbasnia
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001374:Azacitidine / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D050991:GATA4 Transcription Factor / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D006412:Hematopoietic Stem Cells; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction; D013314:Stress, Mechanical
chemical_list: D004791:Enzyme Inhibitors; D050991:GATA4 Transcription Factor; D012333:RNA, Messenger; D001374:Azacitidine
keywords: adipose-derived mesenchymal stem cells; bone marrow-derived mesenchymal stem cells; cardiomyogenic differentiation; equiaxial cyclic strain; real-time PCR