title: Functional interaction between HIV-gp120 and opioid system in the preoptic anterior hypothalamus.
abstract: BACKGROUND
  Recently we found that fever (part of HIV-related wasting) is induced by the action of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (gp120) in the preoptic anterior hypothalamus (POAH). As the opioid system plays a role in the pathogenesis of HIV-1, in the present study we sought to examine the capacity of the opioid system to regulate the febrile response induced by gp120.
  
  
  METHODS
  Stainless steel cannulas were stereotactically into the POAH, and a biotelemetry system was used to monitor the body temperature (Tb changes). We examined the in vivo effects of naloxone as well as highly opioid-selective receptor antagonists, on gp120-induced fever.
  
  
  RESULTS
  Pretreatment with naloxone or the mu-opioid receptor-selective antagonist, cyclic d-Phe-Cys-Tyr-d-Trp-Arg-Thr-Pen-Thr-NH(2) (CTAP), significantly delayed the febrile response induced by gp120. In contrast, naltriben (NTB), a selective antagonist for the delta-2 opioid receptor, did not cause any effect on gp120-induced fever.
  
  
  CONCLUSIONS
  These results (1) provide pharmacologic evidence of a functional in vivo interaction between the opioid system and this viral protein in the POAH and (2) show that mu-opioid receptors can regulate gp120-induced fever.
authors.affiliation: Center for Substance Abuse Research, Temple University School of Medicine, Philadelphia, PA, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Palma
authors.affiliation: Center for Substance Abuse Research, Temple University School of Medicine, Philadelphia, PA, USA.
authors.forename: Mary E
authors.initials: ME
authors.lastname: Abood
authors.affiliation: Department of Anatomy and Cell Biology, Philadelphia, PA, USA.
authors.forename: Mary F
authors.initials: MF
authors.lastname: Barbe
authors.affiliation: Center for Substance Abuse Research, Temple University School of Medicine, Philadelphia, PA, USA. Electronic address: kbenamar@temple.edu.
authors.forename: Khalid
authors.initials: K
authors.lastname: Benamar
mesh_terms: D000818:Animals; D005334:Fever / Q000139:chemically induced / Q000378:metabolism; D015699:HIV Envelope Protein gp120 / Q000502:physiology* / Q000633:toxicity*; D007032:Hypothalamus, Anterior / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009292:Narcotic Antagonists / Q000494:pharmacology*; D011301:Preoptic Area / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017450:Receptors, Opioid, mu / Q000037:antagonists & inhibitors / Q000502:physiology*
chemical_list: D015699:HIV Envelope Protein gp120; D009292:Narcotic Antagonists; D017450:Receptors, Opioid, mu
keywords: Fever; HIV; Opioid system and gp120