title: Pharmacokinetic study of Guanfu base G in rats by LC-ESI-MS.
abstract: A sensitive and simple liquid chromatography -: electrospray ionization mass spectrometry method has been established and validated for the quantification of Guanfu base G in rats. Phenoprolamine hydrochloride was selected as the internal standard. Sample preparation involved simple liquid-liquid extraction by ethylacetate with high efficiency. The chromatographical separation was performed on a Shimadzu C18 column (150 × 2.0 mm, 5 µm) with a gradient elution of 0.2% acetic acid-acetonitrile (30:70, v/v). The method was sensitive with the lowest limit of detection at 1 ng/mL (S/N ≥ 3) in 100 µL of rat plasma. Good linearity (r = 0.9996) was obtained covering a concentration of 5-2000 ng/mL. The intra- and interday assay precision ranged from 4.3 to 6.1% and 5.4 to 8.3%, respectively. In addition, the stability, extraction recovery and matrix effect involved in the method were also validated. After intravenous dosing, rat plasma Guangfu base G (GFG) concentration declined in a biphasic manner with a terminal elimination half-life of 3.72 h. The total plasma clearance values were 1.15 L/h/kg. After oral dosing, the plasma GFG concentration reached a maximum within 0.5 h. The absolute bioavailability of GFG was 83.06%.
authors.affiliation: School of Pharmacy, Zhengzhou University, Zhengzhou 450001, China Luohe Central Hospital Affiliated to Luohe Medical College, Luohe 462000, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Zhengzhou University, Zhengzhou 450001, China lixt@zzu.edu.cn.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Li
authors.affiliation: People's Hospital Affiliated to Zhengzhou University, Zhengzhou 450000, China Henan Provincial People's Hospital, Zhengzhou 450000, China.
authors.forename: Baoluo
authors.initials: B
authors.lastname: Wan
authors.affiliation: School of Pharmacy, Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Yanle
authors.initials: Y
authors.lastname: Chu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001682:Biological Availability; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005260:Female; D006207:Half-Life; D006576:Heterocyclic Compounds, 4 or More Rings / Q000097:blood* / Q000493:pharmacokinetics*; D008297:Male; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D006576:Heterocyclic Compounds, 4 or More Rings; C572618:guanfu base G