title: Simultaneous determination of three major lignans in rat plasma by LC-MS/MS and its application to a pharmacokinetic study after oral administration of Diphylleia sinensis extract.
abstract: A sensitive and selective liquid chromatography tandem mass spectrometry was developed and validated for the simultaneous determination of three major lignans (podophyllotoxin, epipodophyllotoxin, and 4'-demethylpodophyllotoxin) in rat plasma using diphenhydramine as the internal standard. The analytes were detected using a triple quadrupole mass spectrometer that was equipped with an electrospray ionization source in the positive ion and selected reaction monitoring modes. The linearity of the calibration curve was good, with coefficients of determination (r(2) ) >0.9914 for all of the analytes. The developed method was successfully applied for the simultaneous determination of the three lignans in rat plasma following oral administration of Diphylleia sinensis extract to rats.
authors.affiliation: The First Hospital of Jilin University, Changchun, 130021, China.
authors.forename: Chengliang
authors.initials: C
authors.lastname: Zhao
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.forename: Weiyan
authors.initials: W
authors.lastname: He
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.forename: Dan
authors.initials: D
authors.lastname: Shi
authors.forename: Li
authors.initials: L
authors.lastname: Pang
authors.forename: Ning
authors.initials: N
authors.lastname: Dong
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.forename: Honglei
authors.initials: H
authors.lastname: Ji
mesh_terms: D000284:Administration, Oral; D000818:Animals; D028141:Berberidaceae / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D017705:Lignans / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D016014:Linear Models; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D017705:Lignans; D010936:Plant Extracts
keywords: Diphylleia sinensis; LC-MS/MS; lignan; pharmacokinetics; rat plasma