title: Purification of coumarin compounds from Cortex fraxinus by adsorption chromatography.
abstract: In this paper, a chromatographic method for isolation and purification of coumarin compounds from Cortex fraxinus was established by using Superose 12 as the separation media for the first time. The conditions for separation were optimized. Four kinds of coumarin compounds including aesuletin, aesculin, fraxetin and fraxin were obtained. The purity of these compounds were 98.5, 99.1, 97.9 and 97.3%, respectively, which were determined by HPLC area normalization method. The chemical structures of the separated compounds were identified according to (1)H and (13)C nuclear magnetic resonance data. The retention behavior of the separated coumarin compounds on Superose 12 was also discussed. The retention is based on a mixture of hydrogen bonding and hydrophobic interactions between the coumarin compounds and the residues of the cross-linking reagents used in the manufacturing process of Superose 12. The results of this paper indicate that Superose 12 is not only suitable for size-exclusion chromatography of proteins and other biological macromolecules but also for low-molecular-weight natural products.
authors.affiliation: College of Chemistry and Chemical Engineering, Liaocheng University, No. 1, Hunan Road, Liaocheng 252059, China.
authors.forename: Minglan
authors.initials: M
authors.lastname: Yu
authors.affiliation: College of Chemistry and Chemical Engineering, Liaocheng University, No. 1, Hunan Road, Liaocheng 252059, China.
authors.forename: Ailing
authors.initials: A
authors.lastname: Sun
authors.affiliation: Shandong University of Traditional Chinese Medicine, Jinan, China.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Liaocheng University, No. 1, Hunan Road, Liaocheng 252059, China renminliu@163.com liurenmin@lcu.edu.cn.
authors.forename: Renmin
authors.initials: R
authors.lastname: Liu
mesh_terms: D031319:Aesculus; D002850:Chromatography, Gel / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003374:Coumarins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D004929:Esculin / Q000032:analysis / Q000302:isolation & purification; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D012685:Sepharose / Q000737:chemistry; D014468:Umbelliferones / Q000032:analysis
chemical_list: D003374:Coumarins; D004365:Drugs, Chinese Herbal; D014468:Umbelliferones; D004929:Esculin; C517354:horse chestnut seed; D012685:Sepharose; C049225:Superose 12; C105671:fraxetin; C007628:esculetin; C080614:fraxin