title: Expression and motor functional roles of voltage-dependent type 7 K(+) channels in the human taenia coli.
abstract: Voltage-dependent type 7 K(+) (KV7 or KCNQ) channels modulate the excitability of neurons and muscle cells. The aims of the present study were to investigate the motor effects of KV7 channel modulators and the expression of KV7 channels in the human taenia coli. The effects of KV7 channel modulators on the muscle tone of human taenia coli strips were investigated under nonadrenergic non-nitrergic conditions by organ bath studies. Gene expression and tissue localisation of channels were studied by real-time PCR and immunohistochemistry, respectively. Under basal conditions, the KV7 channel blocker XE-991 induced concentration-dependent contractions, with mean EC50 and Emax of 18.7 μM and 30.5% respectively of the maximal bethanechol-induced contraction, respectively. The KV7 channel activators retigabine and flupirtine concentration-dependently relaxed the taenia coli, with mean EC50s of 19.2 μM and 29.9 μM, respectively. Retigabine also relaxed bethanechol-precontracted strips, with maximal relaxations of 79.2% of the bethanecol-induced precontraction. The motor effects induced by the KV7 channel modulators were not affected by tetrodotoxin or ω-conotoxin GVIA. XE-991 greatly reduced retigabine- and flupirtine-induced relaxations. Transcripts encoded by all KCNQ genes were detected in the taenia coli, with KCNQ4 showing the highest expression levels. KV7.4 channels were clearly visualised by immunohistochemistry in colonic epithelium, circular muscle layer and taenia coli. KV7 channels appear to contribute to the resting muscle tone of the human taenia coli. In addition, KV7 channel activators significantly relax the taenia coli. Thus, they could be useful therapeutic relaxant agents for colonic motor disorders.
authors.affiliation: Institute of Pharmacology, School of Medicine, Catholic University of the Sacred Heart, L.go F. Vito 1, 00168 Rome, Italy.
authors.forename: Alice
authors.initials: A
authors.lastname: Adduci
authors.forename: Maria
authors.initials: M
authors.lastname: Martire
authors.forename: Maurizio
authors.initials: M
authors.lastname: Taglialatela
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Arena
authors.forename: Gianluca
authors.initials: G
authors.lastname: Rizzo
authors.forename: Claudio
authors.initials: C
authors.lastname: Coco
authors.forename: Diego
authors.initials: D
authors.lastname: Currò
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000631:Aminopyridines / Q000494:pharmacology; D000873:Anthracenes / Q000494:pharmacology; D002219:Carbamates / Q000494:pharmacology; D003106:Colon / Q000378:metabolism / Q000502:physiology*; D005769:Gastrointestinal Motility / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D051656:KCNQ Potassium Channels / Q000235:genetics* / Q000378:metabolism*; D008875:Middle Aged; D010655:Phenylenediamines / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: C112297:10,10-bis(4-pyridinylmethyl)-9(10H)-anthracenone; D000631:Aminopyridines; D000873:Anthracenes; D002219:Carbamates; D051656:KCNQ Potassium Channels; D010655:Phenylenediamines; D012333:RNA, Messenger; C101866:ezogabine; C034161:flupirtine
keywords: K(V)7 channels; KCNQ channels; Retigabine; taenia coli