title: Clinical significance of NOB1 expression in breast infiltrating ductal carcinoma.
abstract: BACKGROUND
  NIN/RPN Binding protein 1 homologue (NOBp1), encoded by NOB1 gene, was reported to play an essential role in the oncogenesis and prognosis of carcinomas. We conducted a study to reveal its expression and clinical significance in breast infiltrating ductal carcinoma.
  
  
  METHODS
  To explore the relationship between NOB1 expression and the clinical TNM (cTNM), 162 patients who undergone surgery were involved in the study. Compared to healthy tissues, abnormal localization and higher level of NOB1 in tumor cells was observed by Immunohistochemistry staining. Real-time PCR and western-blotting verified the up-regulation of NOB1 in carcinoma individuals.
  
  
  RESULTS
  A significant correlation between high level of NOB1 and the T stage, lymph node metastasis and cTNM was shown. Furthermore, patients with higher level of NOB1 predicted a declined overall survival (OS). Notably, multivariate analyses by Cox's proportional hazard model revealed that expression of NOB1 was an independent prognostic factor in breast infiltrating ductal carcinoma.
  
  
  CONCLUSIONS
  In summary, our present study clarify that the aberrant expression of NOB1 in breast infiltrating ductal carcinoma is possibly involved with tumorigenesis and development, and the NOB1 protein could act as a potential biomarker for prognosis assessment of breast infiltrating ductal carcinoma. Related mechanism is worthy of further investigation.
authors.affiliation: Department of Breast and Thyroid Surgery, Shanghai Tenth People's Hospital, Tongji University Shanghai, 200072, China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Li
authors.forename: Qi-Feng
authors.initials: QF
authors.lastname: Luo
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.forename: Chuan-Kui
authors.initials: CK
authors.lastname: Wei
authors.forename: Xiang-Jie
authors.initials: XJ
authors.lastname: Kong
authors.forename: Jun-Feng
authors.initials: JF
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Fang
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D063646:Carcinogenesis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C506664:NOB1 protein, human; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins
keywords: Breast cancer; NOB1 protein; immunohistochemistry; tissue microarray