title: Effect of (D)-fagomine on excreted Enterobacteria and weight gain in rats fed a high-fat high-sucrose diet.
abstract: OBJECTIVE
  Becoming overweight has been related to elevated levels of Enterobacteriales in the gut. d-Fagomine is an iminosugar that has been shown to selectively agglutinate Enterobacteriales in vitro. The goal of this work is to establish whether d-fagomine exerts a similar effect in vivo and whether this has any downstream consequences on weight gain.
  
  
  METHODS
  The rats were fed a high-fat high-sucrose diet (HFHS) supplemented with d-fagomine (or not; for comparison) or a standard diet for 5 weeks. The levels of total bacteria, Enterobacteriales and Escherichia coli were determined in fecal samples by performing quantitative real-time polymerase chain reactions on DNA.
  
  
  RESULTS
  Whereas the total levels of bacteria were independent of the diet, rats fed HFHS (without d-fagomine) excreted significantly higher proportions of Enterobacteriales and E. coli than those fed a standard diet. The levels of Enterobacteriales and E. coli of the rats given HFHS with d-fagomine were similar to those of the rats fed a standard diet. Compared to the standard group, rats fed HFHS with d-fagomine gained significantly less weight (15.3%) than those fed HFHS (20.9%).
  
  
  CONCLUSIONS
  d-Fagomine reduces the amount of Enterobacteriales excreted by rats fed HFHS and this may help to avert becoming obese.
authors.affiliation: Department of Biological Chemistry and Molecular Modelling, Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), Barcelona, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Ramos-Romero
authors.forename: Eunice
authors.initials: E
authors.lastname: Molinar-Toribio
authors.forename: Livia
authors.initials: L
authors.lastname: Gómez
authors.forename: Jara
authors.initials: J
authors.lastname: Pérez-Jiménez
authors.forename: Marta
authors.initials: M
authors.lastname: Casado
authors.forename: Pere
authors.initials: P
authors.lastname: Clapés
authors.forename: Benjamín
authors.initials: B
authors.lastname: Piña
authors.forename: Josep Lluís
authors.initials: JL
authors.lastname: Torres
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D059305:Diet, High-Fat / Q000009:adverse effects; D019422:Dietary Sucrose / Q000009:adverse effects; D004195:Disease Models, Animal; D004755:Enterobacteriaceae / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects; D005243:Feces / Q000382:microbiology*; D050112:Imino Pyranoses / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D009765:Obesity / Q000209:etiology / Q000517:prevention & control; D051381:Rats; D017207:Rats, Sprague-Dawley; D015430:Weight Gain / Q000187:drug effects*
chemical_list: D019422:Dietary Sucrose; D050112:Imino Pyranoses; C105643:fagomine