title: Photopatterning of self assembled monolayers on oxide surfaces for the selective attachment of biomolecules.
abstract: The immobilization of functional biomolecules to surfaces is a critical process for the development of biosensors for disease diagnostics. In this work we report the patterned attachment of single chain fragment variable (scFv) antibodies to the surface of metal oxides by the photodeprotection of self-assembled monolayers, using near-UV light. The photodeprotection step alters the functionality at the surface; revealing amino groups that are utilized to bind biomolecules in the exposed regions of the substrate only. The patterned antibodies are used for the detection of specific disease biomarker proteins in buffer and in complex samples such as human serum.
authors.affiliation: School of Chemistry, University of Manchester, Oxford Road, Manchester M13 9PL, UK.
authors.forename: Pompi
authors.initials: P
authors.lastname: Hazarika
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Behrendt
authors.forename: Linn
authors.initials: L
authors.lastname: Petersson
authors.forename: Christer
authors.initials: C
authors.lastname: Wingren
authors.forename: Michael L
authors.initials: ML
authors.lastname: Turner
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006801:Humans; D010087:Oxides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties; D014466:Ultraviolet Rays
chemical_list: D055910:Antibodies, Immobilized; D010087:Oxides; D011506:Proteins; D006046:Gold; D012825:Silicon
keywords: Biomarker detection; Photo-pattern; Proteins; Self-assembled monolayer (SAM); Silicon