title: Molecular cloning and expression analysis of pig CD138.
abstract: CD138 (syndecan-1) interacts with various components of the extracellular matrix and associates with the actin cytoskeleton. In this study, we cloned pig CD138 cDNA and determined its complete cDNA sequence. Pig CD138 cDNA contained an open reading frame (930 bp) encoding 309 amino acids with five well conserved putative glycosaminoglycan attachment sites, a putative cleavage site for matrix metalloproteinases, and conserved motifs involved in signal transduction among mammalian species. Pig CD138 mRNA was detected in various tissues, including lymphoid and non-lymphoid organs, indicating the multicellular functions of CD138 in pigs. Western blot and flow cytometry analyses detected an approximate 35 kDa pig CD138 protein expressed on the cell surface. Further immunohistochemistry analysis revealed that CD138 expression was mainly observed in submucosa and lamina propria of the pig small intestine. Further study will be necessary to define the functional importance of CD138 during specific infectious diseases in pigs.
authors.affiliation: Division of Biotechnology, School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Joonbeom
authors.initials: J
authors.lastname: Bae
authors.forename: Seonah
authors.initials: S
authors.lastname: Jeong
authors.forename: Ju Yeon
authors.initials: JY
authors.lastname: Lee
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Lee
authors.forename: Bong-Hwan
authors.initials: BH
authors.lastname: Choi
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Kim
authors.forename: Inho
authors.initials: I
authors.lastname: Choi
authors.forename: Taehoon
authors.initials: T
authors.lastname: Chun
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western / Q000662:veterinary; D003001:Cloning, Molecular; D005434:Flow Cytometry / Q000662:veterinary; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment / Q000662:veterinary; D013552:Swine / Q000235:genetics; D053668:Syndecan-1 / Q000235:genetics* / Q000502:physiology; D014018:Tissue Distribution
chemical_list: D053668:Syndecan-1
keywords: CD138; Expression; Gapdh; Pig; Syndecan; cDNA cloning; glyceraldehyde-3-phosphate dehydrogenase