title: New Sicydiinae phylogeny (Teleostei: Gobioidei) inferred from mitochondrial and nuclear genes: insights on systematics and ancestral areas.
abstract: The Sicydiinae subfamily (Teleostei: Gobioidei) is the biggest contributor to the diversity of fish communities in river systems of tropical islands. These species are found in the Indo-Pacific area, the Caribbean region and West Africa. They spawn in freshwater, their planktotrophic larvae drift downstream to the sea where they develop, before returning to the rivers to grow and reproduce. Hence, they are called amphidromous. Their phylogeny has been explored using a total of 3545 sites from 5 molecular markers (mitochondrial DNA: 16S rDNA, cytochrome oxidase I, cytochrome b; nuclear DNA: rhodopsin gene and a nuclear marker specially developed for this study, the interferon regulatory factor 2 binding protein 1-IRF2PB1). Sequences were obtained for 59 Sicydiinae specimens of 9 known genera. The Bayesian and maximum likelihood analyses support the monophyly of the subfamily as well as the monophylyof all genera except Sicydium, which is paraphyletic. Five major clades were identified within this subfamily. One clade contained the genus Stiphodon. Another clade contained Sicyopterus, Sicydium and Parasicydium with Sicyopterus as sister genus of Sicydium. The non-monophyly of Sicydium subclade, because it includes the monotypic genus Parasicydium, challenged the validity of Parasicydium genus. Ancestral area reconstruction showed that the subfamily emerged in the Central West Pacific region implying that previous hypotheses proposing a dispersal route for Sicydiinae into the Atlantic Ocean are unsupported by the present analysis. Our results suggest that the hypotheses for the dispersal route of the genus Sicydium should be reconsidered.
authors.affiliation: Milieux et Peuplements Aquatiques, Muséum National d'Histoire naturelle - UMR BOREA 7208 (CNRS-IRD-MNHN-UPMC), 57 rue Cuvier CP026, 75231 Paris Cedex 05, France; Research Institute for the Environment and Livelihoods, Charles Darwin University, Darwin 0909, NT, Australia. Electronic address: laura.taillebois@cdu.edu.au.
authors.forename: Laura
authors.initials: L
authors.lastname: Taillebois
authors.forename: Magalie
authors.initials: M
authors.lastname: Castelin
authors.forename: Clara
authors.initials: C
authors.lastname: Lord
authors.forename: Ryan
authors.initials: R
authors.lastname: Chabarria
authors.forename: Agnès
authors.initials: A
authors.lastname: Dettaï
authors.forename: Philippe
authors.initials: P
authors.lastname: Keith
mesh_terms: D000818:Animals; D001499:Bayes Theorem; D002467:Cell Nucleus / Q000378:metabolism; D045303:Cytochromes b / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D008928:Mitochondria / Q000378:metabolism; D010473:Perciformes / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D045303:Cytochromes b; D003576:Electron Transport Complex IV
keywords: Amphidromy; Ancestral areas; Dispersal; Molecular phylogeny; Sicydiinae