title: Screening of testosterone esters in human plasma.
abstract: The detection of an intact ester of testosterone in plasma is leading towards unequivocal proof of the administration of exogenous testosterone. In the current study, a sensitive screening method for the detection of nine testosterone esters in human plasma was developed. By preparing oxime derivatives of intact testosterone esters, the sensitivity of the assay was increased. Furthermore, the method included liquid-liquid extraction (LLE) as sample clean-up, as well as online separation of the target analytes from the derivatization solution. The analysis was performed by liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS). The method developed herein is simple and rapid, and was validated according to World Anti-Doping Agency (WADA) guidelines.
authors.affiliation: Doping Control Laboratory, Seibersdorf Labor GmbH, Seibersdorf, Austria; Department of Pharmacy, University of Tromsø, Tromsø, Norway.
authors.forename: G
authors.initials: G
authors.lastname: Forsdahl
authors.forename: H K
authors.initials: HK
authors.lastname: Vatne
authors.forename: T
authors.initials: T
authors.lastname: Geisendorfer
authors.forename: G
authors.initials: G
authors.lastname: Gmeiner
mesh_terms: D000728:Androgens / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods; D004300:Doping in Sports; D004952:Esters / Q000097:blood; D006801:Humans; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction / Q000379:methods; D010091:Oximes / Q000097:blood; D015813:Substance Abuse Detection / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013739:Testosterone / Q000097:blood*
chemical_list: D000728:Androgens; D004952:Esters; D010091:Oximes; D013739:Testosterone
keywords: doping analysis; mass spectrometry; oxime derivatives; testosterone ester