title: Comparative transcriptional analysis reveals differential gene expression between Sand Daffodil tissues.
abstract: Sand Daffodil (Pancratium maritimum) is a world-wide endangered Amayllidaceae species and represents an important anti-cancer medicinal resource due to alkaloids production. Despite its increasing pharmaceutical importance, there are not molecular resources that can be utilized toward improving genetic traits. In our research, the suppression subtractive hybridization (SSH) method conducted to generate large-scale expressed sequence tags (EST), was designed to identify gene candidates related to the morphological and physiological differences between the two tissues, leaves and bulbs, since lycorine, the main anti-cancer compound, is there synthesized. We focused on identification of transcripts in different tissues from Sand Daffodil using PCR-based suppression SSH to identify genes involved in global pathway control. Sequencing of 2,000 differentially screened clones from the SSH libraries resulted in 136 unigenes. Functional annotation and gene ontology analysis of up-regulated EST libraries showed several known biosynthetic genes and novel transcripts that may be involved in signaling, cellular transport, or metabolism. Real time RT-PCR analysis of a set of 8 candidate genes further confirmed the differential gene expression.
authors.affiliation: DISTABIF-Department of Science and Technology, Environmental, Biological and Pharmaceutical, University of Naples II, Via Vivaldi 43, 81100, Caserta, Italy, bruna.defelice@unina2.it.
authors.forename: Bruna
authors.initials: B
authors.lastname: De Felice
authors.forename: Francesco
authors.initials: F
authors.lastname: Manfellotto
authors.forename: Raffaella
authors.initials: R
authors.lastname: D'Alessandro
authors.forename: Olga
authors.initials: O
authors.lastname: De Castro
authors.forename: Antonietta
authors.initials: A
authors.lastname: Di Maio
authors.forename: Marco
authors.initials: M
authors.lastname: Trifuoggi
mesh_terms: D001483:Base Sequence; D020224:Expressed Sequence Tags; D015870:Gene Expression; D020869:Gene Expression Profiling; D063990:Gene Ontology; D017343:Genes, Plant; D018745:Genome, Plant; D008969:Molecular Sequence Data; D031427:Narcissus / Q000166:cytology* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D009928:Organ Specificity; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D018626:Retroelements; D017422:Sequence Analysis, DNA
chemical_list: D018626:Retroelements