title: Histological changes and immunolabeling of Leishmania infantum in kidneys and urinary bladder of dogs.
abstract: The aim of this study was to assess histological alterations and perform immunolabeling of Leishmania infantum in the kidneys and urinary bladder of naturally infected dogs. Twenty-five urinary bladder and kidney samples of serologically positive animals (ELISA S7® Biogene and IFAT ≥ 1:40 - Biomanguinhos/Fiocruz) were analyzed by means of immunohistochemical and histological techniques. Cystitis was found in 44% (11/25) of the bladder samples and membranoproliferative glomerulonephritis in 92% (23/25) of the kidney samples. Immunolabeling of the parasite revealed that 32% (8/25) of the bladders and 8% (2/25) of the kidneys were positive. In conclusion, the immunohistochemical technique is a useful tool for detecting amastigote forms of L. infantum in organs of infected dogs. In addition, this was the first report of detection of amastigote forms of L. infantum in the bladders of dogs.
authors.affiliation: Universidade Federal Rural de Pernambuco, Departamento de Medicina Veterinária, Laboratório de Doenças Parasitárias dos Animais Domésticos, RecifePE, Brasil.
authors.forename: Jamile Prado
authors.initials: JP
authors.lastname: dos Santos
authors.forename: Leucio Câmara
authors.initials: LC
authors.lastname: Alves
authors.forename: Rafael Antonio Nascimento
authors.initials: RA
authors.lastname: Ramos
authors.forename: Danillo de Souza
authors.initials: Dde S
authors.lastname: Pimentel
authors.forename: Gílcia Aparecida
authors.initials: GA
authors.lastname: de Carvalho
authors.forename: Maria Fernanda Melo
authors.initials: MF
authors.lastname: Monteiro
authors.forename: Maria Aparecida da Gloria
authors.initials: MA
authors.lastname: Faustino
mesh_terms: D000818:Animals; D003556:Cystitis / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D004285:Dogs / Q000469:parasitology*; D015433:Glomerulonephritis, Membranous / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D007150:Immunohistochemistry; D007668:Kidney / Q000469:parasitology* / Q000473:pathology*; D018314:Leishmania infantum / Q000276:immunology* / Q000302:isolation & purification*; D001743:Urinary Bladder / Q000469:parasitology* / Q000473:pathology*