title: In vitro callus induction and plantlet regeneration of Achyranthes aspera L., a high value medicinal plant.
abstract: OBJECTIVE
  To study callus induction from different explants (internode, leaf, root) and in vitro plantlets propagation from medicinally important plant Achyranthes aspera L.
  
  
  METHODS
  Sterilized explants were prepared by using 0.1% HgCl2 and 0.5% Bavistin and callus was obtained when cultured onto Murashige Skoog's (MS) medium by using different concentrations and combination of 2,4-D, NAA, BAP, IAA, IBA with 3% sucrose and 0.8% agar. Induced callus was immediately transferred to MS medium containing at different concentrations of phytohormones for shootlets and rootlets induction respectively.
  
  
  RESULTS
  Sterilization treatment of 0.1% HgCl2 for 2-3 min and Bavistin 0.5% for 10-12 min showed the highest percentage of asepsis and survival rate. Maximum induction of callus was obtained from a combination of 2.0 mg/L 2,4-D and 0.5 mg/L NAA from leaf. Highest shootlets number (4.83±0.17) and length (3.8±0.16) cm were observed on full strength MS medium when fortified with BAP 4.0 mg/L and KIN 0.5 mg/L. Concerted efforts of BAP 2.0 mg/L and NAA 0.5 mg/L on full strength MS medium showed highest leaf number (6.77±0.94). In vitro raised shoots were allowed to root on different strengths of MS medium fortified with IAA and IBA at different concentrations. Experimentally, 3.0 mg/L IBA was enabled to induce maximum rootlets number (10.0±9.82) on full strength MS medium. Afterwards, regenerated shoots with well developed roots were successfully subjected to hardening process and were acclimatized. The survived plantlets showed 66.67% survival frequency without any morphological abnormality.
  
  
  CONCLUSIONS
  The results demonstrated that different explants were good source of callus induction, morphology analysis as well as indirect plantlets regeneration.
authors.affiliation: Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia, Bangladesh.
authors.forename: Monokesh Kumer
authors.initials: MK
authors.lastname: Sen
authors.forename: Shamima
authors.initials: S
authors.lastname: Nasrin
authors.forename: Shahedur
authors.initials: S
authors.lastname: Rahman
authors.forename: Abu Hena Mostofa
authors.initials: AH
authors.lastname: Jamal
mesh_terms: D029044:Achyranthes / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots; D018520:Plant Shoots; D010946:Plants, Medicinal / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D010937:Plant Growth Regulators
keywords: Achyranthes aspera; Callus induction; Micropropagation; Murashige Skoog's; Plantlets propagation