title: Gene expression patterns combined with bioinformatics analysis identify genes associated with cholangiocarcinoma.
abstract: To explore the molecular mechanisms of cholangiocarcinoma (CC), microarray technology was used to find biomarkers for early detection and diagnosis. The gene expression profiles from 6 patients with CC and 5 normal controls were downloaded from Gene Expression Omnibus and compared. As a result, 204 differentially co-expressed genes (DCGs) in CC patients compared to normal controls were identified using a computational bioinformatics analysis. These genes were mainly involved in coenzyme metabolic process, peptidase activity and oxidation reduction. A regulatory network was constructed by mapping the DCGs to known regulation data. Four transcription factors, FOXC1, ZIC2, NKX2-2 and GCGR, were hub nodes in the network. In conclusion, this study provides a set of targets useful for future investigations into molecular biomarker studies.
authors.affiliation: Department of General Surgery, Zhongshan Hospital Affiliated in Fudan University, Shanghai, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.forename: Weixing
authors.initials: W
authors.lastname: Shen
authors.forename: Sheng
authors.initials: S
authors.lastname: Shen
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Ai
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D018281:Cholangiocarcinoma / Q000235:genetics*; D019295:Computational Biology; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins; D006801:Humans; D009687:Nuclear Proteins; D014157:Transcription Factors
chemical_list: D014408:Biomarkers, Tumor; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins; C000715631:NKX2-2 protein, human; D009687:Nuclear Proteins; D014157:Transcription Factors
keywords: Bioinformatics analysis; Cholangiocarcinoma; Differentially coexpressed gene; Regulatory network