title: Melt-electrospun polycaprolactone strontium-substituted bioactive glass scaffolds for bone regeneration.
abstract: Polycaprolactone (PCL) is a resorbable polymer used extensively in bone tissue engineering owing to good structural properties and processability. Strontium-substituted bioactive glass (SrBG) has the ability to promote osteogenesis and may be incorporated into scaffolds intended for bone repair. Here, we describe for the first time, the development of a PCL-SrBG composite scaffold incorporating 10% (weight) of SrBG particles into PCL bulk, produced by the technique of melt electrospinning. We show that we are able to reproducibly manufacture composite scaffolds with an interconnected porous structure and, furthermore, these scaffolds were demonstrated to be noncytotoxic in vitro. Ions present in the SrBG component were shown to dissolve into cell culture media and promoted precipitation of a calcium phosphate layer on the scaffold surface which in turn led to noticeably enhanced alkaline phosphatase activity in MC3T3-E1 cells compared to PLC-only scaffolds. These results suggest that melt-electrospun PCL-SrBG composite scaffolds show potential to become effective bone graft substitutes.
authors.affiliation: Biomaterials and Tissue Morphology Group, Institute of Health & Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, 4059, Australia.
authors.forename: Jiongyu
authors.initials: J
authors.lastname: Ren
authors.forename: Keith A
authors.initials: KA
authors.lastname: Blackwood
authors.forename: Amir
authors.initials: A
authors.lastname: Doustgani
authors.forename: Patrina P
authors.initials: PP
authors.lastname: Poh
authors.forename: Roland
authors.initials: R
authors.lastname: Steck
authors.forename: Molly M
authors.initials: MM
authors.lastname: Stevens
authors.forename: Maria A
authors.initials: MA
authors.lastname: Woodruff
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000737:chemistry*; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D002516:Ceramics / Q000737:chemistry*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry*; D013324:Strontium / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: C022107:Bioglass; D018786:Bone Substitutes; D011091:Polyesters; C016240:polycaprolactone; D000469:Alkaline Phosphatase; D013324:Strontium
keywords: bioactive scaffolds; bone tissue engineering; melt electrospinning; polycaprolactone; strontium-substituted bioactive glass