title: Cytotoxic effect of doxorubicin on human multiple myeloma cells in vitro.
abstract: Cytotoxic effect of doxorubicin in human multiple myeloma cell cultures RPMI 1640, RPMI 8226, and IM-9 was studied. The obtained data were compared with mRNA expression of MDR1, MRP1, BCRP, LRP genes responsible for the development of multiple drug resistance. IM-9 cells that differed from the other two cultures by the expression of surface differentiation markers and by mRNA expression of MDR1, BCRP, and LRP were most sensitive to doxorubicin. All cells expressed mRNA for only A-isoform of insulin receptor (IRA), while B-isoform (IRB) was not expressed. Insulin in a concentration of 5 μg/ml had no effect on the cytotoxic effect of doxorubicin in the studied cells.
authors.affiliation: Institute of Carcinogenesis, N. N. Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia. sainHershy@yandex.ru.
authors.forename: S S
authors.initials: SS
authors.lastname: Shushanov
authors.forename: T A
authors.initials: TA
authors.lastname: Kravtsova
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D004317:Doxorubicin / Q000494:pharmacology*; D018432:Drug Resistance, Multiple / Q000235:genetics*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D026503:Low Density Lipoprotein Receptor-Related Protein-1 / Q000235:genetics; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics; D009101:Multiple Myeloma / Q000188:drug therapy*; D009363:Neoplasm Proteins / Q000235:genetics; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D011972:Receptor, Insulin / Q000235:genetics
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; D000903:Antibiotics, Antineoplastic; D004317:Doxorubicin; D007328:Insulin; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D009363:Neoplasm Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D011972:Receptor, Insulin; C513055:ABCB1 protein, human; C116614:ABCG2 protein, human; C475476:LRP1 protein, human; C421109:multidrug resistance-associated protein 1