title: In vivo time-serial multi-modality optical imaging in a mouse model of ovarian tumorigenesis.
abstract: Identification of the early microscopic changes associated with ovarian cancer may lead to development of a diagnostic test for high-risk women. In this study we use optical coherence tomography (OCT) and multiphoton microscopy (MPM) (collecting both two photon excited fluorescence [TPEF] and second harmonic generation [SHG]) to image mouse ovaries in vivo at multiple time points. We demonstrate the feasibility of imaging mouse ovaries in vivo during a long-term survival study and identify microscopic changes associated with early tumor development. These changes include alterations in tissue microstructure, as seen by OCT, alterations in cellular fluorescence and morphology, as seen by TPEF, and remodeling of collagen structure, as seen by SHG. These results suggest that a combined OCT-MPM system may be useful for early detection of ovarian cancer.
authors.affiliation: Biomedical Engineering; University of Arizona; Tucson, AZ USA.
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Watson
authors.affiliation: Physiology Department; University of Arizona; Tucson, AZ USA.
authors.forename: Samuel L
authors.initials: SL
authors.lastname: Marion
authors.affiliation: Biomedical Engineering; University of Arizona; Tucson, AZ USA.
authors.forename: Photini F
authors.initials: PF
authors.lastname: Rice
authors.affiliation: Biomedical Engineering; University of Arizona; Tucson, AZ USA.
authors.forename: David L
authors.initials: DL
authors.lastname: Bentley
authors.affiliation: University Animal Care; University of Arizona; Tucson, AZ USA.
authors.forename: David G
authors.initials: DG
authors.lastname: Besselsen
authors.affiliation: Biomedical Engineering; University of Arizona; Tucson, AZ USA.
authors.forename: Urs
authors.initials: U
authors.lastname: Utzinger
authors.affiliation: Physiology Department; University of Arizona; Tucson, AZ USA.
authors.forename: Patricia B
authors.initials: PB
authors.lastname: Hoyer
authors.affiliation: Biomedical Engineering; University of Arizona; Tucson, AZ USA.
authors.forename: Jennifer K
authors.initials: JK
authors.lastname: Barton
mesh_terms: D000818:Animals; D063646:Carcinogenesis / Q000473:pathology*; D005260:Female; D006106:Granulosa Cell Tumor / Q000473:pathology; D006965:Hyperplasia / Q000473:pathology; D051379:Mice; D064847:Multimodal Imaging; D010051:Ovarian Neoplasms / Q000473:pathology*; D010053:Ovary / Q000473:pathology*; D059008:Time-Lapse Imaging; D041623:Tomography, Optical Coherence
keywords: autofluorescence; cancer; multiphoton; non-linear microscopy; optical biopsy; optical coherence tomography; two-photon