title: Cloning and molecular characterization of BumaMPs1, a novel metalloproteinases from the venom of scorpion Buthus martensi Karsch.
abstract: Scorpion venoms metalloproteinase is involved in a number of important biological, physiological and pathophysiological processes. In this work, a complete sequence of metalloproteinase was first obtained from venom of scorpion Buthus martensi and named as BumaMPs1. BumaMPs1 has 393 amino acid residues containing with a molecular mass of 44.53 kDa, showing an isoelectric point of 5.66. The primary sequence analysis indicated that the BumaMPs1 contains a zinc-binding motif (HELGHNLGISH), methionine-turn motif (YIM), disintegrin-like domain (ETCD) and N-glycosylation site. The multiple alignment of its deduced amino acid sequence and those of other metalloproteinase showed a high structural similarly, mainly among class reprolysin proteases. The phylogenetic analysis showed early divergence and independent evolution of BumaMPs1 from other metalloproteinase.
authors.affiliation: Basal Medical College of Nanyang Medical University, Nanyang 473041, Henan Province, China. Electronic address: xiaxichao8336@163.com.
authors.forename: Xichao
authors.initials: X
authors.lastname: Xia
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Ma
authors.forename: Shipeng
authors.initials: S
authors.lastname: Xue
authors.forename: Aimei
authors.initials: A
authors.lastname: Wang
authors.forename: Junliang
authors.initials: J
authors.lastname: Tao
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Qingyuan
authors.initials: Q
authors.lastname: Zhang
authors.forename: Rongzhi
authors.initials: R
authors.lastname: Liu
authors.forename: Shaoe
authors.initials: S
authors.lastname: Lu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D003001:Cloning, Molecular; D045726:Metalloproteases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D012604:Scorpion Venoms / Q000737:chemistry; D012605:Scorpions / Q000201:enzymology* / Q000235:genetics; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein
chemical_list: D060829:Arthropod Proteins; D012604:Scorpion Venoms; D045726:Metalloproteases
keywords: Buthus martensi; Cloning and molecular characterization; Metalloproteinase; Phylogenetic tree