title: Glutathione S-transferase, glutathione peroxidase and acetylcholinesterase activities in mussels transplanted to harbour areas.
abstract: As part of an integrative monitoring campaign involving water and sediment chemistry, in situ bioassays, and mussel bioaccumulation and biomarkers, Mytilus galloprovincialis mussels of standard size were transplanted from a clean location to five sites in two important harbours from the Atlantic coast of Spain (Vigo and Pasaia). After a 30-day field exposure, the concentrations of major contaminants (trace metals, polychlorinated biphenyls and polycyclic aromatic hydrocarbons) accumulated in mussel tissues were measured at each site, and a mussel bioaccumulation index (MBI) was calculated. The enzymatic activity levels of glutathione S-transferase (GST), glutathione peroxidase (GPx) and acetylcholinesterase (AChE) were quantified in the gills of transplanted mussels (n=12). Mussels from the most polluted sites consistently exhibited significantly higher GST and GPx activities compared to the control site, whereas AChE activity was significantly inhibited. The responses of the GST and GPx activities were related to MBI, trace metals and PAH concentrations in mussels, whereas AChE activity was related to the trace metals concentrations in mussels. The above results suggest that GST and AChE activities can be used as potential biomarkers for active monitoring in marine coastal ecosystems. However, at this moment, GPx activity is not robust enough to be applicable to harbour areas.
authors.affiliation: Departamento de Ecoloxía e Bioloxía Animal, Universidade de Vigo, Campus Universitario de Vigo, E-36310 Vigo, Galicia, Spain. Electronic address: leticia1582@uvigo.es.
authors.forename: L
authors.initials: L
authors.lastname: Vidal-Liñán
authors.affiliation: Instituto Español de Oceanografía, Centro Oceanográfico de Vigo, E-36200 Vigo, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Bellas
authors.affiliation: Departamento de Química Analítica, Fac. Ciencias & Tecnología, EHU/UPV, Bilbao, P.O. Box 644, E-48080, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Etxebarria
authors.affiliation: Departamento de Quimica Analitica e Alimentaria, Universidade de Vigo, Campus Universitario de Vigo, E-36310 Vigo, Galicia, Spain.
authors.forename: O
authors.initials: O
authors.lastname: Nieto
authors.affiliation: Departamento de Ecoloxía e Bioloxía Animal, Universidade de Vigo, Campus Universitario de Vigo, E-36310 Vigo, Galicia, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Beiras
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004784:Environmental Monitoring; D005979:Glutathione Peroxidase / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D008670:Metals / Q000032:analysis / Q000633:toxicity; D049878:Mytilus / Q000378:metabolism*; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000633:toxicity; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity; D013030:Spain; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity
chemical_list: D015415:Biomarkers; D008670:Metals; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D000110:Acetylcholinesterase
keywords: Acetylcholinesterase; Antioxidant enzymes; Bioaccumulation; Biomarkers; Integrative assessment; Mytilus galloprovincialis