title: Autoclaving of the middle ear ossicles in an animal experimental model.
abstract: CONCLUSIONS
  Autoclaving the ossicles for 4 min at 134°C impacts superficial devitalization and elimination of the squamous epithelium on the surface of the ossicles. Bone maintains the integrity, firmness, and lamellar structure and ossicles are integrated into the middle ear without any adverse reactions.
  
  
  OBJECTIVE
  Sixteen guinea pigs were used to explore the effect of autoclaving on middle ear ossicles.
  
  
  METHODS
  Harvested incudomallear complex was autoclaved for 4 min at 134°C and implanted back into the middle ear cavity. Exploration of the middle ear was carried out 30 days after implantation. Results were compared with a control group of 16 non-autoclaved ossicles.
  
  
  RESULTS
  Autoclaved ossicles maintained their integrity, firmness, shape, and lamellar structure with Haversian systems. Autoclaving substantially decreased the vitality of the ossicles. The middle ear mucosa overgrew on the ossicular surface. No adverse reactions of soft tissue against ossicles occurred. Although the autoclaved ossicles were implanted with tympanic membrane (squamous epithelium) the histological examination did not demonstrate any residuum of squamous epithelium. In the control group we proved the presence of squamous epithelium on the manubrium surface in all cases; all ossicles were vital without signs of ostitis.
authors.affiliation: Department of Otorhinolaryngology and Head and Neck Surgery.
authors.forename: Lukas
authors.initials: L
authors.lastname: Skoloudik
authors.forename: David
authors.initials: D
authors.lastname: Kalfert
authors.forename: Katarina
authors.initials: K
authors.lastname: Zborayova
authors.forename: Jan
authors.initials: J
authors.lastname: Laco
mesh_terms: D000818:Animals; D018424:Cholesteatoma, Middle Ear / Q000473:pathology / Q000601:surgery*; D004195:Disease Models, Animal; D004429:Ear Ossicles / Q000637:transplantation*; D006168:Guinea Pigs; D019933:Ossicular Replacement / Q000379:methods*; D013242:Sterilization / Q000379:methods*; D014182:Transplantation, Autologous; D014432:Tympanic Membrane