title: Zunongwangia mangrovi sp. nov., isolated from mangrove (Avicennia marina) rhizosphere, and emended description of the genus Zunongwangia.
abstract: A Gram-stain-negative, strictly aerobic, slightly halophilic, non-motile and rod-shaped bacterial strain, designated P2E16(T), was isolated from mangrove (Avicennia marina) rhizosphere, collected at Devipattinam mangroves, Tamil Nadu, India. Strain P2E16(T) grew optimally at pH 7.0-8.0, at 25-28 °C and in the presence of 2-3% (w/v) NaCl. 16S rRNA gene analysis showed that strain P2E16(T) was phylogenetically closely related to the genus Zunongwangia, with Zunongwangia profunda SM-A87(T) as the closest related type strain (98.2% 16S rRNA gene sequence similarity) and less than 93% 16S rRNA gene sequence similarity to all other members of the family Flavobacteriaceae. Strain P2E16(T) contained MK-6 as the major respiratory quinone, phosphatidylethanolamine as the predominant polar lipid and iso-C(15 : 0) (17.8%), iso-C(17 : 0) 3-OH (15.1%), C(15 : 0) (12.8%), iso-C(17 : 1)ω9c (9.8%), iso-C(15 : 1) G (9.0%), and summed feature 3 (comprising C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH; 7.1%) as the major fatty acids. The DNA G+C content was 34.3 mol%. Differential phenotypic properties, together with the phylogenetic distinctiveness and low DNA-DNA relatedness demonstrated that strain P2E16(T) was distinct from the type strain of Zunongwangia profunda. On the basis of these presented data, strain P2E16(T) is considered to represent a novel species of the genus Zunongwangia, for which the name Zunongwangia mangrovi sp. nov. is proposed. The type strain is P2E16(T) ( = DSM 24499(T) = LMG 26237(T) = KCTC 23496(T)). An emended description of the genus Zunongwangia is also provided.
authors.affiliation: Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho,Hakodate 041-8611, Japan.|NII Microbial Culture Collection (NIICC), Biotechnology Department, National Institute for Interdisciplinary Science and Technology (CSIR), Thiruvananthapuram - 695 019, Kerala, India.
authors.forename: N
authors.initials: N
authors.lastname: Rameshkumar
authors.affiliation: NII Microbial Culture Collection (NIICC), Biotechnology Department, National Institute for Interdisciplinary Science and Technology (CSIR), Thiruvananthapuram - 695 019, Kerala, India.
authors.forename: Ramya
authors.initials: R
authors.lastname: Krishnan
authors.affiliation: Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7b, 30124 Braunschweig, Germany.
authors.forename: Elke
authors.initials: E
authors.lastname: Lang
authors.affiliation: Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho,Hakodate 041-8611, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Matsumura
authors.affiliation: Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho,Hakodate 041-8611, Japan.
authors.forename: Toko
authors.initials: T
authors.lastname: Sawabe
authors.affiliation: Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho,Hakodate 041-8611, Japan.
authors.forename: Tomoo
authors.initials: T
authors.lastname: Sawabe
mesh_terms: D032407:Avicennia / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007194:India; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6