title: [Screening molecular markers in early breast cancer of the same pathological types but with different prognoses using Agilent gene chip].
abstract: OBJECTIVE
  To screen molecular markers in early breast cancer and establish gene subtyping-based diagnostic criteria for predicting the prognosis of early breast cancers.
  
  
  METHODS
  Tumor tissue specimens were obtained from 8 patients with early breast cancer for analysis of the differentially expressed genes using Agilent custom 8×15 000 chips in combination with the prognostic data of the patients. Another 42 tumor tissue specimens were used to validate the differential genes by real-time fluorescent quantitative PCR.
  
  
  RESULTS
  Gene microarray analysis identified 132 differentially expressed genes between the patients with favorable and poor prognosis, and 44 of these genes were significantly up-regulated (by over two folds) and 88 down-regulated in patients with poor prognoses.
  
  
  CONCLUSIONS
  The gene expression profiles differ in early breast cancer tissues of the same pathological type but with different clinical stages and prognoses, and CD44, MKI67, NTRK2, Nek2, C16orf60, TOP2A, ANCCA, and RRM2 genes can be used as the prognostic markers for early breast cancer.
authors.affiliation: Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. E-mail: leezhou888@126.com.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Li
authors.forename: Liang
authors.initials: L
authors.lastname: Peng
authors.forename: Shuai
authors.initials: S
authors.lastname: Han
authors.forename: Zonghai
authors.initials: Z
authors.lastname: Huang
authors.forename: Fujun
authors.initials: F
authors.lastname: Shi
authors.forename: Zhai
authors.initials: Z
authors.lastname: Cai
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Li
authors.forename: Pusheng
authors.initials: P
authors.lastname: Zhang
authors.forename: Huijuan
authors.initials: H
authors.lastname: Zhu
authors.forename: Weirong
authors.initials: W
authors.lastname: Jin
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor