title: Screening of differentially expressed microRNA in ulcerative colitis related colorectal cancer.
abstract: OBJECTIVE
  To investigate the differential expression of microRNA (miRNA) in colon between ulcerative colitis (UC) and ulcerative colitis related colorectal cancer (UCRCC).
  
  
  METHODS
  An UC mouse model was built by dextran sodium sulfate, and an UCRCC mouse model by dextran sodium sulfate and 1,2-diformylhydrazine. RNAs were extracted from the colon, purified and hybridized with fluorescence-labeled miRNA oligonucleotide gene chip. Real-time fluorescence quantitative PCR was used to verify the expression variation of miRNA. SAM was employed for the data analysis.
  
  
  RESULTS
  The up-regulated miRNAs in colon cancer included has-miR-194, has-miR-215, has-miR-93, has-miR-192, has-miR-92a, has-miR-29b, and has-miR-20a (median false discovery rate<5%), while the down-regulated miRNAs were has-miR-1231, has-miR-195, has-miR-143, and has-miR-145 (median false discovery rate<5%).
  
  
  CONCLUSIONS
  Significant differential expression of miRNA was found between the UC mouse and UCRCC mouse, which may be related to the onset, erosion and transfer of colorectal cancer.
authors.affiliation: Department of Gastroenterology, The Second People's Hospital of Shenzhen (The First Affiliated Hospital of Shenzhen University), Shenzhen 518035, China.
authors.forename: Yong-Gang
authors.initials: YG
authors.lastname: Tan
authors.forename: Yong-Feng
authors.initials: YF
authors.lastname: Zhang
authors.forename: Chang-Jun
authors.initials: CJ
authors.lastname: Guo
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.forename: Man-Yin
authors.initials: MY
authors.lastname: Chen
mesh_terms: D000818:Animals; D003093:Colitis, Ulcerative / Q000235:genetics / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D051379:Mice; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs
keywords: Carcinogenesis; Colorectal cancer; MicroRNA; Microarray; Mouse model; Ulcerative colitis