title: [Examination of cytoprotective and anti-inflammatory effect of PACAP-38 on small bowel autotransplantation].
abstract: BACKGROUND
  The small intestine is one of the most sensitive organs to ischemia-reperfusion injury during transplantation. Cytoprotective effect of pituitary adenylate cyclase-activating polypeptide (PACAP) is well known. The aim of our study was to measure changes of PACAP-38-like immunoreactivities and cytokine levels in intestinal grafts stored PACAP-38 containing preservation solution.
  
  
  METHODS
  Small-bowel autotransplantation was performed on male Wistar rats (n = 56). Grafts were stored in University of Wisconsin (UW) solution at 4 °C for 1 (GI), 3 (GII), and 6 hours (GIII); and in PACAP-38 containing UW solution for 1 (GIV), 3 (GV), and 6 hours (GVI). Reperfusion lasted 3 hours in each group. Intestinal PACAP-38 immunoreactivities were measured by radioimmunoassay. To measure cytokine from tissue homogenates we used rat cytokine array and Luminex Multiplex Immunoassay.
  
  
  RESULTS
  Levels of PACAP-38-like and PACAP-27-like immunoreactivities decreased by preservation time compared to control. This decrease was significant following 6 hours cold storage (p < 0.05). Values remained significantly higher in grafts stored in PACAP-38 containing UW. Expressions of sICAM-1, L-selectin, tissue inhibitor of metalloproteinase-1 were increased in GIII and were decreased in GVI.
  
  
  CONCLUSIONS
  PACAP-38 increased tissue levels of PACAP-38 and PACAP-27, and decreased cytokine expression. This indicates that PACAP-38 has anti-inflammatory and cytoprotective effects in intestinal autotransplantation model.
authors.affiliation: Zala Megyei Kórház Általános Sebészeti és Érsebészeti Osztály Zalaegerszeg.
authors.forename: Klára
authors.initials: K
authors.lastname: Nedvig
authors.forename: Györgyi
authors.initials: G
authors.lastname: Szabó
authors.forename: Domokos
authors.initials: D
authors.lastname: Csukás
authors.forename: József
authors.initials: J
authors.lastname: Sándor
authors.forename: József
authors.initials: J
authors.lastname: Németh
authors.forename: Krisztina
authors.initials: K
authors.lastname: Kovács
authors.forename: Dóra
authors.initials: D
authors.lastname: Reglődi
authors.forename: Agnes
authors.initials: A
authors.lastname: Kemény
authors.forename: György
authors.initials: G
authors.lastname: Wéber
authors.forename: Andrea
authors.initials: A
authors.lastname: Ferencz
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism*; D019610:Cytoprotection / Q000187:drug effects*; D015536:Down-Regulation; D005978:Glutathione; D006085:Graft Survival / Q000187:drug effects*; D006133:Growth Substances / Q000494:pharmacology*; D007328:Insulin; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism / Q000637:transplantation*; D019041:L-Selectin / Q000378:metabolism; D008297:Male; D009926:Organ Preservation / Q000379:methods; D019188:Organ Preservation Solutions; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000378:metabolism* / Q000494:pharmacology*; D011863:Radioimmunoassay; D011887:Raffinose; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000517:prevention & control*; D013997:Time Factors; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D014182:Transplantation, Autologous
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016207:Cytokines; D006133:Growth Substances; D007328:Insulin; D019188:Organ Preservation Solutions; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D019715:Tissue Inhibitor of Metalloproteinase-1; C055206:University of Wisconsin-lactobionate solution; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose
keywords: PACAP-27; PACAP-38; citokinexpressio; cytokine expression; small bowel; transplantation; transzplantáció; vékonybél