title: First report of Enterocytozoon bieneusi from dairy cattle in Argentina.
abstract: Fecal specimens were obtained from a total of 70 dairy calves less than two months old on 11 municipalities in Buenos Aires, Argentina. After removal of fecal debris by sieving and sucrose flotation, specimens were subjected to PCR to detect the presence of Enterocytozoon bieneusi. PCR revealed a 14.3% of prevalence for E. bieneusi with 10 positive calves from 7 municipalities. Gene sequence analysis conducted in all samples positives by PCR revealed the presence of six genotypes; four previously reported in cattle as well as humans (D, I, J, and BEB4), one never reported in cattle before but previously reported in humans (EbpC), and one novel genotype (BEB10). These results constitute the first molecular characterization of E. bieneusi in Argentina, and suggest a potential risk of zoonotic transmission in this area.
authors.affiliation: Cátedra de Microbiología y Parasitología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, 60 y 120, La Plata 1900, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Electronic address: vdelcoco@aetos.med.unlp.edu.ar.
authors.forename: Valeria F
authors.initials: VF
authors.lastname: Del Coco
authors.forename: María Alejandra
authors.initials: MA
authors.lastname: Córdoba
authors.forename: Gladys
authors.initials: G
authors.lastname: Bilbao
authors.forename: Pinto
authors.initials: P
authors.lastname: de Almeida Castro
authors.forename: Juan Angel
authors.initials: JA
authors.lastname: Basualdo
authors.forename: Mónica
authors.initials: M
authors.lastname: Santín
mesh_terms: D000818:Animals; D001118:Argentina / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D003612:Dairying; D021862:Enterocytozoon / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D005243:Feces / Q000382:microbiology; D005838:Genotype; D006801:Humans; D016881:Microsporidiosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D008969:Molecular Sequence Data
chemical_list: D021903:DNA, Ribosomal Spacer
keywords: Argentina; Cattle; Enterocytozoon bieneusi; Genotype; Microsporidia; PCR