title: Satellite glial cell proliferation in the trigeminal ganglia after chronic constriction injury of the infraorbital nerve.
abstract: We have examined satellite glial cell (SGC) proliferation in trigeminal ganglia following chronic constriction injury of the infraorbital nerve. Using BrdU labeling combined with immunohistochemistry for SGC specific proteins we positively confirmed proliferating cells to be SGCs. Proliferation peaks at approximately 4 days after injury and dividing SGCs are preferentially located around neurons that are immunopositive for ATF-3, a marker of nerve injury. After nerve injury there is an increase GFAP expression in SGCs associated with both ATF-3 immunopositive and immunonegative neurons throughout the ganglia. SGCs also express the non-glial proteins, CD45 and CD163, which label resident macrophages and circulating leukocytes, respectively. In addition to SGCs, we found some Schwann cells, endothelial cells, resident macrophages, and circulating leukocytes were BrdU immunopositive.
authors.affiliation: University of California San Francisco, Center for Integrative Neuroscience, BOX 0444, 675 Nelson Rising Lane, San Francisco, California.
authors.forename: Macayla
authors.initials: M
authors.lastname: Donegan
authors.forename: Melanie
authors.initials: M
authors.lastname: Kernisant
authors.forename: Criselda
authors.initials: C
authors.lastname: Cua
authors.forename: Luc
authors.initials: L
authors.lastname: Jasmin
authors.forename: Peter T
authors.initials: PT
authors.lastname: Ohara
mesh_terms: D051700:Activating Transcription Factor 3 / Q000378:metabolism; D000818:Animals; D049109:Cell Proliferation; D003250:Constriction; D008297:Male; D059348:Peripheral Nerve Injuries / Q000378:metabolism / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D027161:Satellite Cells, Perineuronal / Q000378:metabolism / Q000502:physiology*; D012668:Trigeminal Ganglion / Q000378:metabolism / Q000502:physiology*
chemical_list: D051700:Activating Transcription Factor 3
keywords: BrdU; Trigeminal nerve; immune cells; nerve injury; neuropathic pain; trigeminal ganglion