title: Development and characterization of 14 microsatellite loci in the Neotropical fish Geophagus brasiliensis (Perciformes, Cichlidae).
abstract: Fourteen polymorphic microsatellite loci were isolated and characterized for the Neotropical cichlid Geophagus brasiliensis and tested on 30 individuals belonging to a single population. Among the 14 loci described, four showed potential presence of null alleles, inferred from the excess of homozygous genotypes, and three of these loci showed significant deviations from Hardy-Weinberg equilibrium. Fifty-nine different alleles were detected (ranging from two to eight alleles per locus), with estimates of observed and expected heterozygosity ranging from 0·167 to 0·700 and from 0·269 to 0·825. Cross-amplification of primers was successful in five other cichlid species.
authors.affiliation: Departamento de Biologia Geral, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, km 380, Londrina, PR, 86051-980, Brazil.
authors.forename: D G
authors.initials: DG
authors.lastname: Ferreira
authors.forename: B A
authors.initials: BA
authors.lastname: Galindo
authors.forename: A N
authors.initials: AN
authors.lastname: Alves
authors.forename: F S
authors.initials: FS
authors.lastname: Almeida
authors.forename: C F
authors.initials: CF
authors.lastname: Ruas
authors.forename: S H
authors.initials: SH
authors.lastname: Sofia
mesh_terms: D000483:Alleles; D000818:Animals; D017931:DNA Primers; D015723:Gene Library; D006579:Heterozygote; D018895:Microsatellite Repeats; D010473:Perciformes / Q000235:genetics*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers
keywords: Geophagini; SSR; genetic marker; genomic library