title: ACE-inhibitory activity of enzymatic protein hydrolysates from lupin and other legumes.
abstract: The objective of this investigation was to compare the angiotensin converting enzyme (ACE)-inhibitory activity of the hydrolysates obtained by pepsin digestion of proteins of some legumes, such as chickpea, common bean, lentil, lupin, pea, and soybean, by using the same experimental procedure. The ACE-inhibitory activity was measured by using the tripeptide hippuryl-histidyl-leucine (HHL), as model peptide, and HPLC-DAD, as analytical method. The peptide mixtures of all legumes were active, with soybean and lupin the most efficient, with IC50 values of 224 and 226 μg/ml, respectively. Considering the promising results obtained with lupin, and aiming to identify the protein(s) that release(s) the peptides responsible for the activity, the peptides obtained from the pepsin digestion of some industrial lupin protein isolates and purified protein fractions were tested. The most active mixture, showing an IC50 value of 138 μg/ml, was obtained hydrolysing a mixture of lupin α+β conglutin.
authors.affiliation: Laboratory of Food Chemistry and Mass Spectrometry, Department of Pharmaceutical Sciences, Università degli Studi di Milano, via Mangiagalli 25, 20133 Milano, Italy. Electronic address: giovanna.boschin@unimi.it.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Boschin
authors.forename: Graziana Maria
authors.initials: GM
authors.lastname: Scigliuolo
authors.forename: Donatella
authors.initials: D
authors.lastname: Resta
authors.forename: Anna
authors.initials: A
authors.lastname: Arnoldi
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007887:Fabaceae / Q000737:chemistry*; D031297:Lupinus / Q000737:chemistry*; D010455:Peptides / Q000494:pharmacology*; D011492:Protein Hydrolysates / Q000494:pharmacology*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D010455:Peptides; D011492:Protein Hydrolysates
keywords: ACE; Angiotensin converting enzyme (ACE) inhibitors; BSA; E/S; Enzymatic protein hydrolysates; HA; HHL; HL; Hippuric acid; Hypertension; LPI; Legumes; Lupin; Pepsin; Protein isolates; SDS–PAGE; TPE; angiotensinconverting enzyme; bovin serum albumin; enzyme/substrate; hippuric acid; hippuryl-histidyl-leucine; histidyl-leucine; lupin protein isolate; sodium dodecyl sulphate–polyacrylamide gel electrophoresis; total protein extract