title: Characterization of the small RNA transcriptome in plant-microbe (Brassica/Erwinia) interactions by high-throughput sequencing.
abstract: Non-coding, small RNAs (sRNAs) have been identified in a wide spectrum of organisms ranging from bacteria to humans; however, the role and mechanisms of these sRNA in plant immunity is largely unknown. To determine possible roles of sRNA in plant-pathogen interaction, we carried out a high-throughput sRNA sequencing of Brassica campestris using non-infected plants and plants infected with Erwinia carotovora. Consistent with our hypothesis that distinct classes of host sRNAs alerts their expression levels in response to infection, we found that: (1) host 28-nt sRNAs were strongly increased under pathogen infection; and (2) a group of host sRNAs homologous to the pathogen genome also accumulated at significantly higher level. Our data thus suggest several distinct classes of the host sRNAs may enhance their function by up-regulation of their expression/stability in response to bacterial pathogen challenges.
authors.affiliation: Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, 200031, China, cbsun@sibs.ac.cn.
authors.forename: Chuan Bao
authors.initials: CB
authors.lastname: Sun
mesh_terms: D001937:Brassica / Q000235:genetics / Q000382:microbiology*; D054884:Host-Pathogen Interactions; D016973:Pectobacterium carotovorum / Q000254:growth & development*; D058727:RNA, Small Untranslated / Q000096:biosynthesis*; D059467:Transcriptome
chemical_list: D058727:RNA, Small Untranslated