title: [Effect of mild to moderate hypothermia on casepase-3, Bcl-2 and Bax expressions in brain tissue of rats after cardiopulmonary resuscitation].
abstract: OBJECTIVE
  To explore the effect of mild to moderate hypothermia on the expressions of apoptosis-related genes in the brain tissue of rats after cardiopulmonary resuscitation (CPR).
  
  
  METHODS
  CPR models were established by asphyxia in 15 male SD rats, which were randomized equally into normal temperature group, 34 degrees celsius hypothermia group and 32 degrees celsius hypothermia group. The brain tissues of the rats were obtained after treatment for 12 h to observe the pathological changes. The expression of caspase-3 in cerebral cortex neurons was determined with immunohistochemistry, and the expressions of Bcl-2 and Bax were detected by Western blotting.
  
  
  RESULTS
  Compared with normal temperature group, the two hypothermia groups (especially 32 degrees celsius group) showed significantly decreased expression of caspase-3 in the cortical neurons (P<0.05). Bcl-2 protein expression was significantly increased in the hypothermia groups, especially in 32 degrees celsius hypothermia group (P<0.05). There was no significant difference in Bax protein expression among the 3 groups.
  
  
  CONCLUSIONS
  Mild hypothermia can relieve brain injury by down-regulating caspase-3 expression and up-regulating Bcl-2 protein expression to inhibit apoptosis of the brain neurons. Hypothermia at 32 degrees celsius offers better protection of the brain tissue than hypothermia at 34 degrees celsius.
authors.affiliation: Department of Emergency Medicine, Xiangya Hospital, Central South University, Changsha 410008, China. E-mail: 1493616174@qq.com.
authors.forename: Qingchun
authors.initials: Q
authors.lastname: He
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.forename: Liping
authors.initials: L
authors.lastname: Zhou
authors.forename: Aimin
authors.initials: A
authors.lastname: Wang
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D016887:Cardiopulmonary Resuscitation; D053148:Caspase 3 / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism* / Q000473:pathology; D007036:Hypothermia, Induced; D008297:Male; D009474:Neurons / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
chemical_list: C494828:Bax protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C505410:Casp3 protein, rat; D053148:Caspase 3