title: Tissue-specific IL-10 secretion profile from term human fetal membranes stimulated with pathogenic microorganisms associated with preterm labor in a two-compartment tissue culture system.
abstract: OBJECTIVE
  Interleukin (IL)-10 is a cytokine with anti-inflammatory properties that plays pivotal roles in immune recognition and maintenance of pregnancy, limiting the harmful effects of pro-inflammatory modulators. The aim of this work was to characterize the contribution of amnion and choriodecidua regions of the human fetal membranes in the production of IL-10 after selective stimulation with Candida albicans, Gardnerella vaginalis and Streptococcus agalactiae.
  
  
  METHODS
  Pre-labor human fetal membranes were cultured in a two-compartment tissue culture system and stimulated with 1 × 10(6) CFU/ml of each pathogen added to either the amniotic or choriodecidual region or both.
  
  
  RESULTS
  Candida albicans and G. vaginalis were the pathogens most effective in inducing IL-10 secretion, increasing 20 and 10 times, respectively, the levels of this cytokine in the choriodecidual compartment. Stimulation with S. agalactiae was effective only in the choriodecidual region, increasing two times IL-10 concentration.
  
  
  CONCLUSIONS
  Synthesis and secretion of IL-10 in response to three different pathogens associated with intrauterine infection and preterm birth are differential and depend on the nature of the microorganism and initial contact region.
authors.affiliation: Cell Biology Branch .
authors.forename: Verónica
authors.initials: V
authors.lastname: Zaga-Clavellina
authors.forename: Pilar
authors.initials: P
authors.lastname: Flores-Espinosa
authors.forename: Montzerrat
authors.initials: M
authors.lastname: Pineda-Torres
authors.forename: Irma
authors.initials: I
authors.lastname: Sosa-González
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Vega-Sánchez
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Estrada-Gutierrez
authors.forename: Aurora
authors.initials: A
authors.lastname: Espejel-Núñez
authors.forename: Arturo
authors.initials: A
authors.lastname: Flores-Pliego
authors.forename: Rolando
authors.initials: R
authors.lastname: Maida-Claros
authors.forename: Higinio
authors.initials: H
authors.lastname: Estrada-Juárez
authors.forename: Angel
authors.initials: A
authors.lastname: Chávez-Mendoza
mesh_terms: D000650:Amnion / Q000276:immunology* / Q000378:metabolism; D002176:Candida albicans; D002821:Chorioamnionitis / Q000276:immunology / Q000382:microbiology; D002823:Chorion / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006194:Gardnerella vaginalis; D006801:Humans; D066298:In Vitro Techniques; D016753:Interleukin-10 / Q000378:metabolism*; D007752:Obstetric Labor, Premature / Q000276:immunology / Q000382:microbiology; D011247:Pregnancy; D013292:Streptococcus agalactiae; D046509:Tissue Culture Techniques
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10
keywords: Chorioamnionitis; IL-10; human fetal membranes; intrauterine infection; preterm labor