title: Effect of pH on cellulase production and morphology of Trichoderma reesei and the application in cellulosic material hydrolysis.
abstract: A low-cost of cellulase achieved through improving fermentation technology remains a key requirement for commercialization of cellulosic biofuels and biochemicals. pH plays a very important role in the process of cellulase synthesis by Trichoderma reesei. In this work, effects of pH on the production and production rates of three cellulase components (endoglucanase, exoglucanase, β-glucosidase) and mycelial morphology were studied. Production rates of the cellulase components were kept highest and the mycelial morphology was maintained at the optimal status by developing a phased pH control strategy in order to improve cellulase production. Cellulase production in terms of filter paper activity and β-glucosidase production in batch fermentation increased 17.6% and 22%. Saccharification efficiency of the enzyme obtained by pH control was evaluated by hydrolyzing pretreated corn cob. Saccharification yield increased significantly (up to 26.2%) compared with that without pH control. These results add new knowledge on approach for improving cellulase production.
authors.affiliation: Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Yang
authors.forename: Ronglin He Can
authors.initials: RH
authors.lastname: Zhang
authors.forename: Dongyuan
authors.initials: D
authors.lastname: Zhang
authors.forename: Shulin
authors.initials: S
authors.lastname: Chen
authors.forename: Lijuan
authors.initials: L
authors.lastname: Ma
mesh_terms: D001709:Biotechnology / Q000379:methods; D002480:Cellulase / Q000096:biosynthesis* / Q000737:chemistry; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D014242:Trichoderma / Q000201:enzymology; D003313:Zea mays / Q000201:enzymology*; D001617:beta-Glucosidase / Q000096:biosynthesis*
chemical_list: D001617:beta-Glucosidase; D002480:Cellulase
keywords: Cellulase; Hydrolysis; Protein; pH; β-Glucosidase