title: Association of biofilm production with multidrug resistance among clinical isolates of Acinetobacter baumannii and Pseudomonas aeruginosa from intensive care unit.
abstract: OBJECTIVE
  Given choice, bacteria prefer a community-based, surface-bound colony to an individual existence. The inclination for bacteria to become surface bound is so ubiquitous in diverse ecosystems that it suggests a strong survival strategy and selective advantage for surface dwellers over their free-ranging counterparts. Virtually any surface, biotic or abiotic (animal, mineral, or vegetable) is suitable for bacterial colonization and biofilm formation. Thus, a biofilm is "a functional consortium of microorganisms organized within an extensive exopolymeric matrix."
  
  
  METHODS
  The present study was undertaken to detect biofilm production from the repertoire stocks of Acinetobacter baumannii (A. baumannii) and Pseudomonas aeruginosa (P. aeruginosa) obtained from clinical specimens. The tube method was performed to qualitatively detect biofilm production.
  
  
  RESULTS
  A total of 109 isolates of both organisms were included in the study, out of which 42% (46/109) isolates showed biofilm detection. Among the biofilm producers, 57% of P. aeruginosa and 73% of A. baumannii showed multidrug resistance (MDR) pattern which was statistically significant in comparison to nonbiofilm producers (P < 0.001).
  
  
  CONCLUSIONS
  To the best of our knowledge, this is the only study to have tested the biofilm production in both P. aeruginosa and A. baumannii in a single study. Biofilm production and MDR pattern were found to be significantly higher in A. baumannii than P. aeruginosa. Antibiotic resistance was significantly higher among biofilm producing P. aeruginosa than non producers. Similarly, antibiotic resistance was significantly higher among biofilm producing A. baumannii than non producers.
authors.affiliation: Department of Microbiology, North Eastern Indira Gandhi Regional Institute of Health and Medical Sciences, Mawdiangdiang, Shillong, India.
authors.forename: Jeetendra
authors.initials: J
authors.lastname: Gurung
authors.forename: Annie Bakorlin
authors.initials: AB
authors.lastname: Khyriem
authors.forename: Amit
authors.initials: A
authors.lastname: Banik
authors.forename: Wihiwot Valarie
authors.initials: WV
authors.lastname: Lyngdoh
authors.forename: Basabdatta
authors.initials: B
authors.lastname: Choudhury
authors.forename: Prithwis
authors.initials: P
authors.lastname: Bhattacharyya
keywords: Acinetobacter baumannii; Pseudomonas aeruginosa; biofilm; multidrug resistant