title: Sample preparation: a critical step in the analysis of cholesterol oxidation products.
abstract: In recent years, cholesterol oxidation products (COPs) have drawn scientific interest, particularly due to their implications on human health. A big number of these compounds have been demonstrated to be cytotoxic, mutagenic, and carcinogenic. The main source of COPs is through diet, and particularly from the consumption of cholesterol-rich foods. This raises questions about the safety of consumers, and it suggests the necessity for the development of a sensitive and a reliable analytical method in order to identify and quantify these components in food samples. Sample preparation is a necessary step in the analysis of COPs in order to eliminate interferences and increase sensitivity. Numerous publications have, over the years, reported the use of different methods for the extraction and purification of COPs. However, no method has, so far, been established as a routine method for the analysis of COPs in foods. Therefore, it was considered important to overview different sample preparation procedures and evaluate the different preparative parameters, such as time of saponification, the type of organic solvents for fat extraction, the stationary phase in solid phase extraction, etc., according to recovery, precision and simplicity.
authors.affiliation: Department of Chemistry, University of Cyprus, 1678 Nicosia, Cyprus.
authors.forename: Christiana A
authors.initials: CA
authors.lastname: Georgiou
authors.forename: Michalis S
authors.initials: MS
authors.lastname: Constantinou
authors.forename: Constantina P
authors.initials: CP
authors.lastname: Kapnissi-Christodoulou
mesh_terms: D053000:Analytic Sample Preparation Methods; D002776:Cholestanes / Q000009:adverse effects / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D002784:Cholesterol / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D002791:Cholesterol, Dietary / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D003257:Consumer Product Safety; D004852:Epoxy Compounds / Q000009:adverse effects / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination; D006868:Hydrolysis; D006888:Hydroxycholesterols / Q000009:adverse effects / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D007653:Ketocholesterols / Q000009:adverse effects / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D059625:Liquid-Liquid Extraction; D010084:Oxidation-Reduction; D052616:Solid Phase Extraction
chemical_list: D002776:Cholestanes; D002791:Cholesterol, Dietary; D004852:Epoxy Compounds; D006888:Hydroxycholesterols; D007653:Ketocholesterols; D002784:Cholesterol
keywords: Cholesterol oxidation products; Lipid extraction; Sample preparation; Saponification; Solid phase extraction