title: Light-emitting diode spectral sensitivity relationship with reproductive parameters and ovarian maturation in yellowtail damselfish, Chrysiptera parasema.
abstract: The present study investigated the effects of exposure to different light spectra and intensities on ovarian maturation in yellowtail damselfish, Chrysiptera parasema over a 4-months period. We used a white fluorescent bulb and three different light-emitting diodes (LEDs: red, peak at 630nm; green, 530nm; blue, 450nm), at three different intensities each (0.3, 0.6, and 0.9W/m(2)). The effects of different illuminations were assessed by measuring the mRNA and protein expressions of vitellogenin (VTG) and estrogen receptor (ER), gonadosomatic index (GSI), and plasma estradiol-17β (E2) hormone level. For green and blue lights, significantly higher levels of VTG and ER expressions, GSI, and plasma E2 were obtained, compared to the other light spectra. Histological analysis revealed the presence of vitellogenic oocytes in fish exposed to short wavelengths (green and blue) light. In addition, we observed significantly greater ovarian maturation in fish exposed to low and medium light intensities. The results indicate that exposure to green low intensity lighting accelerates gonadal maturation, and is likely to facilitate development of more energy-efficient aquaculture procedures.
authors.affiliation: Division of Marine Environment & BioScience, Korea Maritime and Ocean University, Busan 606-791, Republic of Korea. Electronic address: shin@hhu.ac.kr.
authors.forename: Hyun Suk
authors.initials: HS
authors.lastname: Shin
authors.forename: Na Na
authors.initials: NN
authors.lastname: Kim
authors.forename: Young Jae
authors.initials: YJ
authors.lastname: Choi
authors.forename: Hamid R
authors.initials: HR
authors.lastname: Habibi
authors.forename: Jae Won
authors.initials: JW
authors.lastname: Kim
authors.forename: Cheol Young
authors.initials: CY
authors.lastname: Choi
mesh_terms: D000818:Animals; D049628:Body Size / Q000528:radiation effects; D004958:Estradiol / Q000097:blood; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000528:radiation effects; D008027:Light; D008099:Liver / Q000378:metabolism / Q000528:radiation effects; D010053:Ovary / Q000254:growth & development* / Q000528:radiation effects*; D010473:Perciformes / Q000097:blood / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D012098:Reproduction / Q000528:radiation effects*; D012741:Sexual Maturation / Q000528:radiation effects; D014819:Vitellogenins / Q000235:genetics / Q000378:metabolism
chemical_list: D011960:Receptors, Estrogen; D014819:Vitellogenins; D004958:Estradiol
keywords: Intensity; Light-emitting diodes; Sexual maturation; Short wavelength; Yellowtail damselfish