title: Intracellular microenvironment responsive polymers: a multiple-stage transport platform for high-performance gene delivery.
abstract: A new strategy for promoting endoplasmic gene delivery and nucleus uptake is proposed by developing intracellular microenvironment responsive biocompatible polymers. This delivery system can efficiently load and self-assemble nucleic acids into nano-structured polyplexes at a neutral pH, release smaller imidazole-gene complexes from the polymer backbones at intracellular endosomal pH, transport nucleic acids into nucleus through intracellular environment responsive multiple-stage gene delivery, thus leading to a high cell transfection efficiency.
authors.affiliation: School of Chemical Engineering, The University of Adelaide, Adelaide, SA5005, Australia.
authors.forename: Bingyang
authors.initials: B
authors.lastname: Shi
authors.forename: Hu
authors.initials: H
authors.lastname: Zhang
authors.forename: Sheng
authors.initials: S
authors.lastname: Dai
authors.forename: Xin
authors.initials: X
authors.lastname: Du
authors.forename: Jingxiu
authors.initials: J
authors.lastname: Bi
authors.forename: Shi Zhang
authors.initials: SZ
authors.lastname: Qiao
mesh_terms: D060833:Cellular Microenvironment; D048271:Chitosan / Q000737:chemistry; D004247:DNA / Q000648:ultrastructure; D018014:Gene Transfer Techniques; D006367:HeLa Cells; D006801:Humans; D007093:Imidazoles / Q000737:chemistry; D010316:Particle Size; D010957:Plasmids / Q000648:ultrastructure; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D012545:Schiff Bases / Q000737:chemistry; D014162:Transfection
chemical_list: D007093:Imidazoles; D011108:Polymers; D012545:Schiff Bases; D004247:DNA; D048271:Chitosan
keywords: controlled gene release; intracellular microenvironment responsive; multiple-stage gene delivery; nucleus uptake