title: Atg18 lifts up from and lands on the vacuolar membrane mediated by phosphorylation of its propellers.
abstract: Pichia pastoris Atg18 (PpAtg18), a member of the PROPPIN family of proteins, is localized not only to the PAS (pre-autophagosomal structure or phagophore assembly site) during autophagy but also to the vacuolar membrane during vacuolar fission. Recently we reported that the localization of Atg18 was determined by its phosphorylation level. We identified two phosphorylated regions within the ?-propeller structures of PpAtg18, whose modification affects its affinity toward phosphatidylinositol 3,5-bisphosphate [PtdIns(3,5)P 2]. The findings indicated that phosphoregulaton of Atg18 mediates the signal from various environmental stimuli and regulates its intracellular localization for vacuolar fission and autophagy.
authors.affiliation: Division of Applied Life Sciences; Graduate School of Agriculture; Kyoto University, Kyoto, Japan.
authors.forename: Masahide
authors.initials: M
authors.lastname: Oku
authors.forename: Naoki
authors.initials: N
authors.lastname: Tamura
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Sakai
mesh_terms: D000071183:Autophagy-Related Proteins; D047389:Casein Kinase I / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D010766:Phosphorylation; D010843:Pichia / Q000378:metabolism; D021381:Protein Transport; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism*
chemical_list: C441008:ATG18 protein, S cerevisiae; D000071183:Autophagy-Related Proteins; D005656:Fungal Proteins; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D047389:Casein Kinase I; C479667:YCK3 protein, S cerevisiae
keywords: Atg18; PROPPIN; Pichia pastoris; WIPI; micropexophagy; phosphoinosoitide; phosphorylation; vacuole