title: Generation of neural progenitors from induced Bama miniature pig pluripotent cells.
abstract: Pig pluripotent cells may represent an advantageous experimental tool for developing therapeutic application in the human biomedical field. However, it has previously been proven to be difficult to establish from the early embryo and its pluripotency has not been distinctly documented. In recent years, induced pluripotent stem (iPS) cell technology provides a new method of reprogramming somatic cells to pluripotent state. The generation of iPS cells together with or without certain small molecules has become a routine technique. However, the generation of iPS cells from pig embryonic tissues using viral infections together with small molecules has not been reported. Here, we reported the generation of induced pig pluripotent cells (iPPCs) using the iPS technology in combination with valproic acid (VPA). VPA treatment significantly increased the expression of pluripotent genes and played an important role in early reprogramming. We showed that iPPCs resembled pig epiblast cells in their morphology and pluripotent markers, such as OCT4, NANOG, and SSEA1. It had a normal karyotype and could form embryoid bodies, which express three germ layer markers in vitro. In addition, the iPPCs might directly differentiate into neural progenitors after being induced with the retinoic acid and extracellular matrix. Our study established a reasonable method to generate pig pluripotent cells, which might be a new donor cell source for human neural disease therapy.
authors.affiliation: Department of Histology and Embryology, Harbin Medical University, 194 Xuefu Road, Nangang District, Harbin, Heilongjiang Province, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.forename: Zhi-Yan
authors.initials: ZY
authors.lastname: Shan
authors.forename: Yan-Shuang
authors.initials: YS
authors.lastname: Wu
authors.forename: Xing-Hui
authors.initials: XH
authors.lastname: Shen
authors.forename: Chun-Jia
authors.initials: CJ
authors.lastname: Liu
authors.forename: Jing-Ling
authors.initials: JL
authors.lastname: Shen
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Lei
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000235:genetics; D058953:Neural Stem Cells / Q000166:cytology*; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology*; D013552:Swine; D013556:Swine, Miniature
chemical_list: D050814:Octamer Transcription Factor-3