title: Mechanical characterization of ultrasonically synthesized microbubble shells by flow cytometry and AFM.
abstract: The mechanical properties of the shell of ultrasonically synthesized lysozyme microbubbles, LSMBs, were evaluated by acoustic interrogation and nanoindentation techniques. The Young's modulus of LSMBs was found to be 1.0 ± 0.3 MPa and 0.6 ± 0.1 MPa when analyzed by flow cytometry and AFM, respectively. The shell elasticity and Young's modulus were not affected by the size of the microbubbles (MBs). The hydrogel-like protein shell of LSMBs offers a softer, more elastic and viscous interface compared to lipid-shelled MBs. We show that the acoustic interrogation technique is a real-time, fast, and high-throughput method to characterize the mechanical characteristics of air-filled microbubbles coated by a variety of materials.
authors.affiliation: School of Chemistry, The University of Melbourne , Parkville, Melbourne, Victoria 3010, Australia.
authors.forename: Francesca
authors.initials: F
authors.lastname: Cavalieri
authors.forename: James P
authors.initials: JP
authors.lastname: Best
authors.forename: Camilo
authors.initials: C
authors.lastname: Perez
authors.forename: Juan
authors.initials: J
authors.lastname: Tu
authors.forename: Frank
authors.initials: F
authors.lastname: Caruso
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Matula
authors.forename: Muthupandian
authors.initials: M
authors.lastname: Ashokkumar
mesh_terms: D004548:Elasticity; D005434:Flow Cytometry; D055595:Mechanical Phenomena; D045423:Microbubbles; D018625:Microscopy, Atomic Force; D009113:Muramidase / Q000737:chemistry / Q000648:ultrastructure; D014465:Ultrasonics
chemical_list: D009113:Muramidase