title: Oligopeptide m13 phage display in pathogen research.
abstract: Phage display has become an established, widely used method for selection of peptides, antibodies or alternative scaffolds. The use of phage display for the selection of antigens from genomic or cDNA libraries of pathogens which is an alternative to the classical way of identifying immunogenic proteins is not well-known. In recent years several new applications for oligopeptide phage display in disease related fields have been developed which has led to the identification of various new antigens. These novel identified immunogenic proteins provide new insights into host pathogen interactions and can be used for the development of new diagnostic tests and vaccines. In this review we focus on the M13 oligopeptide phage display system for pathogen research but will also give examples for lambda phage display and for applications in other disease related fields. In addition, a detailed technical work flow for the identification of immunogenic oligopeptides using the pHORF system is given. The described identification of immunogenic proteins of pathogens using oligopeptide phage display can be linked to antibody phage display resulting in a vaccine pipeline.
authors.affiliation: Technische Universität Braunschweig, Institut für Biochemie, Biotechnologie und Bioinformatik, Abteilung Biotechnologie, Spielmannstr. 7, 38106 Braunschweig, Germany. m.hust@tu-bs.de.
authors.forename: Jonas
authors.initials: J
authors.lastname: Kügler
authors.forename: Jonas
authors.initials: J
authors.lastname: Zantow
authors.forename: Torsten
authors.initials: T
authors.lastname: Meyer
authors.forename: Michael
authors.initials: M
authors.lastname: Hust
mesh_terms: D000941:Antigens / Q000235:genetics / Q000302:isolation & purification; D017104:Bacteriophage M13 / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics; D061505:Cell Surface Display Techniques / Q000379:methods*; D009842:Oligopeptides / Q000235:genetics*
chemical_list: D000941:Antigens; D009842:Oligopeptides