title: Genomic editing opens new avenues for zebrafish as a model for neurodegeneration.
abstract: Zebrafish has become a popular model organism to study human diseases. We will highlight the advantages and limitations of zebrafish as a model organism to study neurodegenerative diseases and introduce zinc finger nucleases, transcription activator-like effector nucleases, and the recently established clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeat-associated system for genome editing. The efficiency of the novel genome editing tools now greatly facilitates knock-out and, importantly, also makes knock-in approaches feasible in zebrafish. Genome editing in zebrafish avoids unspecific phenotypes caused by off-target effects and toxicity as frequently seen in conventional knock-down approaches.
authors.affiliation: German Center for Neurodegenerative Diseases (DZNE), Munich, Germany; Munich Cluster for Systems Neurology (SyNergy), Munich, Germany.
authors.forename: Bettina
authors.initials: B
authors.lastname: Schmid
authors.forename: Christian
authors.initials: C
authors.lastname: Haass
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D003851:Deoxyribonucleases / Q000235:genetics; D004195:Disease Models, Animal; D055879:Gene Knock-In Techniques; D055786:Gene Knockout Techniques; D005818:Genetic Engineering / Q000379:methods*; D016678:Genome; D006801:Humans; D019636:Neurodegenerative Diseases / Q000235:genetics*; D015027:Zebrafish / Q000235:genetics*; D016335:Zinc Fingers
chemical_list: D003851:Deoxyribonucleases
keywords: CRISPR/Cas (clustered regularly interspaced short palindromic repeats); TALEN; genomics; neurodegeneration; zebrafish