title: Studies on the role of osteopontin-1 in endometrial cancer cell lines.
abstract: BACKGROUND
  Osteopontin-1 is a well characterized protein in many tumour entities. Multiple roles in the processes invasion, metastasis and angiogenesis of tumours are attributed to osteopontin-1. The putative role of osteopontin-1 has not been characterized for endometrial cancer.
  
  
  METHODS
  We investigated multiple endometrial cancer cell lines for osteopontin-1 mRNA- and protein-expression. Osteopontin-1 dependent effects were analysed in vitro by siRNA inhibition.
  
  
  RESULTS
  All endometrial cell lines expressed osteopontin-1. Expression of osteopontin-1 was successfully inhibited by specific siRNA. Cells with reduced osteopontin-1 expression showed decreased migration in the Boyden chamber assay and invasion was reduced in the wound-healing assay. Osteopontin-1 seems to play a role in apoptotic processes of endometrial cancer cells. Inhibition of osteopontin-1 expression was associated with an increased susceptibility for radiation therapy.
  
  
  CONCLUSIONS
  Osteopontin-1 seems to play a role in endometrial cancer. Inhibition of osteopontin-1 expression leads to a higher susceptibility for radiation therapy. Our results suggest that a reduced expression of osteopontin-1 in endometrial cancer could inhibit the development of invasion and metastasis in these cells.
authors.affiliation: Department of Gynecology, Frauenklinik und Poliklinik, University of Wuerzburg, Josef-Schneider-Str. 4, 97080, Würzburg, Germany.
authors.forename: J C
authors.initials: JC
authors.lastname: Hahne
authors.forename: S R
authors.initials: SR
authors.lastname: Meyer
authors.forename: P
authors.initials: P
authors.lastname: Kranke
authors.forename: J
authors.initials: J
authors.lastname: Dietl
authors.forename: M
authors.initials: M
authors.lastname: Guckenberger
authors.forename: B
authors.initials: B
authors.lastname: Polat
authors.forename: A
authors.initials: A
authors.lastname: Hönig
mesh_terms: D017209:Apoptosis / Q000528:radiation effects*; D045744:Cell Line, Tumor; D016889:Endometrial Neoplasms / Q000378:metabolism* / Q000532:radiotherapy*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects*; D006801:Humans; D053495:Osteopontin / Q000378:metabolism*; D011829:Radiation Dosage
chemical_list: D053495:Osteopontin