title: Immunoglobulin heavy chain gene rearrangement in oral B cell lymphomas.
abstract: OBJECTIVE
  Oral non-Hodgkin lymphomas (NHLs) are an extensive group of malignant lymphoid cell neoplasms that are the second most common group of oral cancers. Subtyping NHL is important to plan for appropriate treatment, and the analysis of clonality is in many instances used for helping in the diagnosis of NHL. Thus, the aim of this study was to analyze immunoglobulin heavy chain (IgH) gene rearrangement in a series of oral B cell lymphomas to investigate the sensitivity of seminested polymerase chain reaction (snPCR).
  
  
  METHODS
  Paraffin embedded tissue samples from 16 cases of oral B cell lymphomas were retrieved and subjected to snPCR to investigate the IgH gene rearrangement.
  
  
  RESULTS
  The results showed monoclonal IgH rearrangement in 85.7% of the cases studied, as represented by finding one band within the expected range of amplification.
  
  
  CONCLUSIONS
  This study found that snPCR is a consistent method for the detection of gene rearrangement in paraffin-embedded tissue.
authors.affiliation: Department of Oral Pathology, Dental School, University of São Paulo, São Paulo, São Paulo, Brazil.
authors.forename: Maria Fernanda Setubal Destro
authors.initials: MF
authors.lastname: Rodrigues
authors.forename: Ricardo Alves
authors.initials: RA
authors.lastname: Mesquita
authors.forename: Lília Alves
authors.initials: LA
authors.lastname: Rocha
authors.forename: Fabio Daumas
authors.initials: FD
authors.lastname: Nunes
authors.forename: Suzana Cantanhede Orsini Machado
authors.initials: SC
authors.lastname: de Sousa
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D006801:Humans; D007150:Immunohistochemistry; D016393:Lymphoma, B-Cell / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics*; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction