title: Boronic Acid-based approach for separation and immobilization of glycoproteins and its application in sensing.
abstract: Glycoproteins influence a broad spectrum of biological processes including cell-cell interaction, host-pathogen interaction, or protection of proteins against proteolytic degradation. The analysis of their glyco-structures and concentration levels are increasingly important in diagnosis and proteomics. Boronic acids can covalently react with cis-diols in the oligosaccharide chains of glycoproteins to form five- or six-membered cyclic esters. Based on this interaction, boronic acid-based ligands and materials have attracted much attention in both chemistry and biology as the recognition motif for enrichment and chemo/biosensing of glycoproteins in recent years. In this work, we reviewed the progress in the separation, immobilization and detection of glycoproteins with boronic acid-functionalized materials and addressed its application in sensing.
authors.affiliation: College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, Henan, China. xianing82414@csu.edu.cn.
authors.forename: Xiaojin
authors.initials: X
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Xia
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
mesh_terms: D001897:Boronic Acids / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D006801:Humans; D007103:Immobilization / Q000379:methods
chemical_list: D001897:Boronic Acids; D006023:Glycoproteins