title: Direct light-up of cAMP derivatives in living cells by click reactions.
abstract: 8-Azidoadenosine 3',5'-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells.
authors.affiliation: Research Center for Advanced Science and Technology, The University of Tokyo 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan. xuyan@fc.miyazaki-u.ac.jp.
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Ito
authors.forename: Hongshan
authors.initials: H
authors.lastname: Liu
authors.forename: Makoto
authors.initials: M
authors.lastname: Komiyama
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Hayashi
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
mesh_terms: D001386:Azides / Q000737:chemistry* / Q000378:metabolism; D057930:Click Chemistry; D000242:Cyclic AMP / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D061565:Cycloaddition Reaction; D034242:Cyclooctanes / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D059010:Single-Cell Analysis / Q000379:methods; D013050:Spectrometry, Fluorescence; D021241:Spectrometry, Mass, Electrospray Ionization; D013194:Staining and Labeling
chemical_list: D001386:Azides; D034242:Cyclooctanes; D005456:Fluorescent Dyes; C017042:8-azidoadenosine-3',5'-monophosphate; D000242:Cyclic AMP