title: The protective effects of fucosterol against skin damage in UVB-irradiated human dermal fibroblasts.
abstract: Exposure to ultraviolet (UV) light causes matrix metalloproteinase (MMP) overexpression and extracellular matrix depletion, leading to skin photoaging. The activation of MMP is related to increased interlukin-6 (IL-6) and type I procollagen production, which is regulated by transforming growth factor-β1 (TGF-β1). Activator protein-1 (AP-1) activation induces MMP-1 production and reduces type I procollagen secretion. Fucosterol, which is extracted and purified from the brown algae Hizikia fusiformis, is a phytosterol. We assessed the effects of fucosterol on photodamage and investigated its molecular mechanism of action in UVB-irradiated normal human dermal fibroblasts by using enzyme-linked immunosorbent assay, Western blot analysis, and reverse transcription-polymerase chain reaction. Our results showed that fucosterol significantly decreased the UVB-induced expression of MMP-1, IL-6, p-c-Jun, and p-c-Fos. Additionally, fucosterol markedly increased the UVB-induced production of type I procollagen and TGF-β1. Our results indicate that fucosterol regulates MMP-1 and type I procollagen expression by modulating AP-1 and TGF-β1 signaling and that MMP-1 activation is correlated with IL-6. These data suggest that fucosterol is a promising botanical agent to protect against skin photodamage.
authors.affiliation: Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggido, 446-701, Republic of Korea.
authors.forename: Eunson
authors.initials: E
authors.lastname: Hwang
authors.forename: Sang-Yong
authors.initials: SY
authors.lastname: Park
authors.forename: Zheng-wang
authors.initials: ZW
authors.lastname: Sun
authors.forename: Heon-Sub
authors.initials: HS
authors.lastname: Shin
authors.forename: Don-Gil
authors.initials: DG
authors.lastname: Lee
authors.forename: Tae Hoo
authors.initials: TH
authors.lastname: Yi
mesh_terms: D015153:Blotting, Western; D020405:Dermis / Q000166:cytology*; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000187:drug effects* / Q000528:radiation effects*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D011832:Radiation Injuries / Q000517:prevention & control*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013265:Stigmasterol / Q000031:analogs & derivatives* / Q000627:therapeutic use; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D015850:Interleukin-6; C015896:fucosterol; D013265:Stigmasterol; D020781:Matrix Metalloproteinase 1