title: Selective exo-enzymatic labeling of N-glycans on the surface of living cells by recombinant ST6Gal I.
abstract: A game of tag: N-Glycans on the surface of living cells were selectively tagged by exogenously administering recombinant ST6Gal I sialyltransferase and azide-modified CMP-Neu5Ac. This modification was followed by a strain-promoted cycloaddition using a biotin-modified dibenzylcyclooctynol (red star=biotin). The methodology will make it possible to dissect the mechanisms that underlie altered glycoconjugate recycling and storage in disease.
authors.affiliation: Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602 (USA); Department of Chemistry, University of Georgia (USA).
authors.forename: Ngalle Eric
authors.initials: NE
authors.lastname: Mbua
authors.forename: Xiuru
authors.initials: X
authors.lastname: Li
authors.forename: Heather R
authors.initials: HR
authors.lastname: Flanagan-Steet
authors.forename: Lu
authors.initials: L
authors.lastname: Meng
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Aoki
authors.forename: Kelley W
authors.initials: KW
authors.lastname: Moremen
authors.forename: Margreet A
authors.initials: MA
authors.lastname: Wolfert
authors.forename: Richard
authors.initials: R
authors.lastname: Steet
authors.forename: Geert-Jan
authors.initials: GJ
authors.lastname: Boons
mesh_terms: D057930:Click Chemistry / Q000379:methods; D005347:Fibroblasts / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D019158:N-Acetylneuraminic Acid; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012794:Sialic Acids / Q000138:chemical synthesis / Q000737:chemistry; D012799:Sialyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
chemical_list: D011134:Polysaccharides; D011994:Recombinant Proteins; D012794:Sialic Acids; D012799:Sialyltransferases; D019158:N-Acetylneuraminic Acid; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
keywords: N-glycans; bioorganic chemistry; click chemistry; glycosyltransferase; sialic acid