title: Myricetin suppresses lipoteichoic acid-induced interleukin-1β and cyclooxygenase-2 expression in human gingival fibroblasts.
abstract: Periodontitis is an inflammatory disease affecting the connective tissue and supporting bone surrounding the teeth. In periodontitis, human gingival fibroblasts (HGFs) synthesize IL-1β, causing a progressive inflammatory response. Flavones demonstrate a variety of biological activity: among others, they possess anti-inflammatory properties. Myricetin is a flavone with a strong anti-inflammatory activity. The objective of this study was to evaluate the effect of the flavonoid myricetin on HGFs under inflammatory conditions induced by lipoteichoic acid (LTA). the effect of myricetin on HGFs was assessed by measuring cell viability, signaling pathways and IL-1β expression and synthesis. It was found that, over time, myricetin did not affect cell viability. However, it inhibited activation of p38 and extracellular-signal-regulated kinase-1/2 in LTA-treated HGFs and also blocked IκB degradation and cyclooxygenase-2 and prostaglandin E2 synthesis and expression. These findings suggest that myricetin has therapeutic effects in the form of controlling LTA-induced inflammatory responses.
authors.affiliation: Faculty of Dentistry, Division of Graduate Studies and Research, Laboratory of Biochemistry, National Autonomous University of Mexico, Federal District, Mexico D. F., 04510, Mexico.
authors.forename: Gloria
authors.initials: G
authors.lastname: Gutiérrez-Venegas
authors.forename: Oscar
authors.initials: O
authors.lastname: Alonso Luna
authors.forename: Jairo Agustín
authors.initials: JA
authors.lastname: Ventura-Arroyo
authors.forename: Cristina
authors.initials: C
authors.lastname: Hernández-Bermúdez
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000096:biosynthesis*; D005347:Fibroblasts / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D005419:Flavonoids / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000276:immunology*; D015398:Signal Transduction / Q000187:drug effects; D013682:Teichoic Acids / Q000276:immunology*
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D007375:Interleukin-1; D008070:Lipopolysaccharides; D013682:Teichoic Acids; C009900:lipoteichoic acid; C040015:myricetin; D051546:Cyclooxygenase 2
keywords: cyclooxygenase-2; interleukin-1; lipoteichoic acid; protein kinase