title: Expression of INHβA and INHβB proteins in porcine oocytes cultured in vitro is dependent on the follicle size.
abstract: The current study aimed to investigate differential expression of inhibin βA (INHβA) and inhibin βB (INHβB) in porcine oocytes before or after in vitro maturation (IVM) isolated from follicles of various sizes. Porcine oocytes isolated from large, medium and small follicles (40 from each) were used to study the INHβA and INHβB protein expression pattern using western blot analysis before or after 44 h of oocyte IVM. An increased expression of INHβA was found in oocytes collected from large and medium follicles compared with small follicles before or after IVM (P < 0.001, P < 0.05, respectively). Similarly, higher INHβB levels were observed in oocytes recovered from large follicles compared with small (P < 0.01). As INHβA and INHβB are expressed in both porcine follicular somatic cells and oocytes, it can be assumed that these transforming growth factor beta (TGFβ) superfamily factors are involved in the regulation of molecular bi-directional pathways during follicle and oocyte development, and can be recognized as markers of follicle and oocyte maturation. Moreover, the current study clearly demonstrated that inhibin expression is substantially associated with porcine follicle growth and development.
authors.affiliation: Department of Histology and Embryology,Department of Anatomy,Poznan University of Medical Sciences,Poznan,Poland.
authors.forename: Bartosz
authors.initials: B
authors.lastname: Kempisty
authors.affiliation: Department of Toxicology,Poznan University of Medical Sciences,60-631 Poznan,Poland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Piotrowska
authors.affiliation: Department of Veterinary Medicine,Poznan University of Life Sciences,60-628,Poznan,Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Rybska
authors.affiliation: Department of Toxicology,Poznan University of Medical Sciences,60-631 Poznan,Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Woźna
authors.affiliation: Department of Veterinary Medicine,Poznan University of Life Sciences,60-628,Poznan,Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Antosik
authors.affiliation: Department of Veterinary Medicine,Poznan University of Life Sciences,60-628,Poznan,Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Bukowska
authors.affiliation: Department of Histology and Embryology,Poznan University of Medical Sciences,60-781 Poznan,Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Zawierucha
authors.affiliation: Department of Histology and Embryology,Poznan University of Medical Sciences,60-781 Poznan,Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Ciesiółka
authors.affiliation: Department of Veterinary Medicine,Poznan University of Life Sciences,60-628,Poznan,Poland.
authors.forename: Jędrzej M
authors.initials: JM
authors.lastname: Jaśkowski
authors.affiliation: Department of Histology and Embryology,Poznan University of Medical Sciences,60-781 Poznan,Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Nowicki
authors.affiliation: Institute of Reproductive Biology,Department of Experimental Reproductive Biology,Leibniz Institute for Farm Animal Biology,Dummerstorf,Germany.
authors.forename: Klaus-Peter
authors.initials: KP
authors.lastname: Brüssow
authors.affiliation: Department of Histology and Embryology,Poznan University of Medical Sciences,60-781 Poznan,Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Zabel
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D059471:In Vitro Oocyte Maturation Techniques; D028322:Inhibin-beta Subunits / Q000378:metabolism*; D009865:Oocytes / Q000502:physiology*; D006080:Ovarian Follicle / Q000166:cytology* / Q000502:physiology; D034421:Sus scrofa
chemical_list: C103008:inhibin beta A subunit; D028322:Inhibin-beta Subunits