title: Acetyl salicylic acid locally enhances functional recovery after sciatic nerve transection in rat.
abstract: Local effect of acetyl salicylic acid (ASA) on peripheral nerve regeneration was studied using a rat sciatic nerve transection model. Forty-five male healthy White Wistar rats were divided into three experimental groups (n = 15), randomly: Sham-operation (SHAM), control (SIL), and ASA-treated (SIL/ASA) groups. In SHAM group after anesthesia left sciatic nerve was exposed through a gluteal muscle incision and after homeostasis the muscle was sutured. In SIL group the left sciatic nerve was exposed the same way and transected proximal to tibio-peroneal bifurcation leaving a 10-mm gap. Proximal and distal stumps were each inserted into a silicone tube and filled with 10 μl phosphate buffered solution. In SIL/ASA group defect was bridged using a silicone tube filled with 10 μl acetyl salisylic acid (0.1 mg/ml). Each group was subdivided into three subgroups of five animals each and were studied 4, 8, and 12 weeks after surgery. Data were analyzed statistically by factorial analysis of variance (ANOVA) and the Bonferroni test for pair-wise comparisons. Functional study confirmed faster and better recovery of regenerated axons in SIL/ASA than in SIL group (p < 0.05). Gastrocnemius muscle mass in SIL/ASA was significantly more than in SIL group. Morphometric indices of regenerated fibers showed that the number and diameter of the myelinated fibers in SIL/ASA were significantly higher than in control group. In immuohistochemistry, location of reactions to S-100 in SIL/ASA was clearly more positive than in SIL group. Response to local treatment of ASA demonstrates that it influences and improves functional recovery of peripheral nerve regeneration.
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University.
authors.forename: Rahim
authors.initials: R
authors.lastname: Mohammadi
authors.forename: Keyvan
authors.initials: K
authors.lastname: Amini
authors.forename: Mehdi
authors.initials: M
authors.lastname: Abdollahi-Pirbazari
authors.forename: Alireza
authors.initials: A
authors.lastname: Yousefi
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001241:Aspirin / Q000008:administration & dosage / Q000627:therapeutic use*; D019771:Axotomy; D004353:Drug Evaluation, Preclinical; D048091:Guided Tissue Regeneration / Q000295:instrumentation / Q000379:methods; D008297:Male; D008866:Microsurgery; D018482:Muscle, Skeletal / Q000294:innervation; D009133:Muscular Atrophy / Q000517:prevention & control; D009413:Nerve Fibers, Myelinated / Q000187:drug effects / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000187:drug effects*; D059348:Peripheral Nerve Injuries / Q000188:drug therapy* / Q000473:pathology; D011183:Postoperative Complications / Q000517:prevention & control; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function; D012584:Sciatic Nerve / Q000293:injuries* / Q000601:surgery / Q000648:ultrastructure; D054457:Tissue Scaffolds; D016138:Walking
chemical_list: D001241:Aspirin