title: Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia.
abstract: Snake liver has been implicated in the adaptation of snakes to a variety of habitats. However, to date, there has been no systematic analysis of snake liver proteins. In this study, we undertook a proteomic analysis of liver from the colubrid snake Elaphe taeniura using a combination of two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flightmass spectrometry (MALDI-TOF MS). We also constructed a local protein sequence database based on transcriptome sequencing to facilitate protein identification. Of the 268 protein spots revealed by 2-DE 109 gave positive MS signals, 84 of which were identified by searching the NCBInr, Swiss-Prot and local databases. The other 25 protein spots could not be identified, possibly because their transcripts were not be stable enough to be detected by transcriptome sequencing. GO analysis showed that most proteins may be involved in binding, catalysis, cellular processes and metabolic processes. Forty-two of the liver proteins identified were found in other reptiles and in amphibians. The findings of this study provide a good reference map of snake liver proteins that will be useful in molecular investigations of snake physiology and adaptation.
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment, College of Life Sciences, Sichuan University, Chengdu, Sichuan Province, China .
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Hengchuan
authors.initials: H
authors.lastname: Xia
authors.forename: Yiting
authors.initials: Y
authors.lastname: Wang
authors.forename: Keping
authors.initials: K
authors.lastname: Chen
authors.forename: Lvgao
authors.initials: L
authors.lastname: Qin
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Qin
authors.initials: Q
authors.lastname: Yao
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Yuanqing
authors.initials: Y
authors.lastname: He
authors.forename: Ermi
authors.initials: E
authors.lastname: Zhao
keywords: 2D electrophoresis; Elaphe taeniura; MALDI-TOF mass spectrometry; protein