title: A highly sensitive long-wavelength fluorescence probe for nitroreductase and hypoxia: selective detection and quantification.
abstract: A novel long-wavelength fluorescence probe has been developed for the detection of nitroreductase (NTR) and hypoxia. could be activated by NTR at 0.1 μM to release the fluorophore and significant changes in fluorescence emission at 658 nm were observed. This feature makes it advantageous for imaging hypoxic cells with minimal endogenous interference.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China. xhqian@ecsut.edu.cn yfxu@ecust.edu.cn.
authors.forename: Ting
authors.initials: T
authors.lastname: Guo
authors.forename: Lei
authors.initials: L
authors.lastname: Cui
authors.forename: Jiaoning
authors.initials: J
authors.lastname: Shen
authors.forename: Weiping
authors.initials: W
authors.lastname: Zhu
authors.forename: Yufang
authors.initials: Y
authors.lastname: Xu
authors.forename: Xuhong
authors.initials: X
authors.lastname: Qian
mesh_terms: D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D000860:Hypoxia; D015394:Molecular Structure; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology; D009601:Nitroreductases / Q000032:analysis* / Q000378:metabolism; D010078:Oxazines / Q000737:chemistry* / Q000378:metabolism; D010100:Oxygen / Q000032:analysis* / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D010078:Oxazines; C008619:Nile Blue; D009601:Nitroreductases; D010100:Oxygen