title: Enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis.
abstract: A gas chromatography-mass spectrometry method for quantification of polyhydroxyalkanoates (PHAs), containing 4-carbon to 16-carbon monomers, even in the absence of standards, was developed. Strong linear correlations existed between PHA carbon number and retention time/response factor (R(2) ≥ 0.987). Based on the correlations, high recovery values, between 100.5% and 114.3%, were obtained for PHA polymers.
authors.affiliation: Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore; Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore. Electronic address: amytangy@ntu.edu.sg.
authors.forename: Giin-Yu Amy
authors.initials: GY
authors.lastname: Tan
authors.affiliation: Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore.
authors.forename: Chia-Lung
authors.initials: CL
authors.lastname: Chen
authors.affiliation: Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore.
authors.forename: Liya
authors.initials: L
authors.lastname: Ge
authors.affiliation: Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.affiliation: Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore; Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore; Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore; Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.
authors.forename: Yu
authors.initials: Y
authors.lastname: Mo
authors.affiliation: Natural Sciences and Science Education Academic Group, Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, Singapore.
authors.forename: Swee Ngin
authors.initials: SN
authors.lastname: Tan
authors.affiliation: Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore; Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore. Electronic address: jywang@ntu.edu.sg.
authors.forename: Jing-Yuan
authors.initials: JY
authors.lastname: Wang
mesh_terms: D001419:Bacteria / Q000737:chemistry; D002244:Carbon / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D054813:Polyhydroxyalkanoates / Q000032:analysis*
chemical_list: D054813:Polyhydroxyalkanoates; D002244:Carbon
keywords: 3-Hydroxyalkanoic acid; Gas chromatography; Homologous; Mass spectrometry; Polyhydroxyalkanoate