title: Comparative expression of thioredoxin-1 in uterine leiomyomas and myometrium.
abstract: Uterine leiomyomas are benign tumors that develop from smooth muscle cells (SMCs). The reactive oxygen species (ROS) have been shown to be involved in the signaling pathways that stimulate proliferation of a variety of cell types. Thioredoxin-1 (TRX-1) is a redox-regulating protein, which is overexpressed in various tumors. In the present study, we investigated the expressions of TRX-1 and its related molecules in uterine leiomyomas and matched adjacent myometrium. Our results showed the expression of TRX-1 was increased in leiomyomas compared with the matched adjacent myometrium by quantitative RT-PCR and western blotting. FOXO3A expression was increased in leiomyomas compared with myometrium by western blotting. The mRNA levels of hypoxia-inducible factor-1α, cyclooxygenase-2 and cyclin D1 were increased in leiomyomas compared with the adjacent myometrium. The mRNA level of (thioredoxin-1-binding protein) TBP-2 in leiomyomas was not altered when compared with the matched adjacent myometrium. These results suggest that TRX-1 and some of its related molecules are associated with the pathogenesis of uterine leiomyomas. The identification of TRX-1 signaling pathways leading to cell proliferation points to another potential therapeutic target for treatment and/or prevention of uterine leiomyomas.
authors.affiliation: Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Hou
authors.forename: Lu
authors.initials: L
authors.lastname: Zhao
authors.forename: Ye
authors.initials: Y
authors.lastname: Li
authors.forename: Fucheng
authors.initials: F
authors.lastname: Luo
authors.forename: Shengdong
authors.initials: S
authors.lastname: Wang
authors.forename: Junying
authors.initials: J
authors.lastname: Song
authors.forename: Jie
authors.initials: J
authors.lastname: Bai
mesh_terms: D000328:Adult; D015153:Blotting, Western; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D005260:Female; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism; D007889:Leiomyoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D008875:Middle Aged; D009215:Myometrium / Q000378:metabolism* / Q000473:pathology; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D035182:TATA Box Binding Protein-Like Proteins / Q000235:genetics / Q000378:metabolism; D013879:Thioredoxins / Q000235:genetics* / Q000378:metabolism; D014594:Uterine Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000601:surgery
chemical_list: C499517:FOXO3 protein, human; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009687:Nuclear Proteins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D035182:TATA Box Binding Protein-Like Proteins; C480637:TBPL2 protein, human; C491203:TXN protein, human; D019938:Cyclin D1; D013879:Thioredoxins; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human
keywords: cyclin D1; cyclooxygenase-2 (COX-2); hypoxia-inducible factor-1α (HIF-1α); thioredoxin-1 (TRX-1); uterine leiomyoma