title: Synthetic brominated furanone F202 prevents biofilm formation by potentially human pathogenic Escherichia coli O103:H2 and Salmonella ser. Agona on abiotic surfaces.
abstract: OBJECTIVE
  Investigate the use of a synthetic brominated furanone (F202) against the establishment of biofilm by Salmonella ser. Agona and E. coli O103:H2 under temperature conditions relevant for the food and feed industry as well as under temperature conditions optimum for growth.
  
  
  RESULTS
  Effect of F202 on biofilm formation by Salmonella ser. Agona and E. coli O103:H2 was evaluated using a microtiter plate assay and confocal microscopy. Effect of F202 on bacterial motility was investigated using swimming and swarming assays. Influence on flagellar synthesis by F202 was examined by flagellar staining. Results showed that F202 inhibited biofilm formation without being bactericidal. F202 was found to affect both swimming and swarming motility without, however, affecting the expression of flagella.
  
  
  CONCLUSIONS
  F202 showed its potential as a biofilm inhibitor of Salmonella ser. Agona and E. coli O103:H2 under temperature conditions relevant for the feed and food industry as well as temperatures optimum for growth. One potential mode of action of F202 was found to be by targeting flagellar function.
  
  
  CONCLUSIONS
  The present study gives valuable new knowledge to the potential use of furanones as a tool in biofilm management in the food and feed industry.
authors.affiliation: Norwegian Veterinary Institute, Department of Laboratory Services, Section for Bacteriology- aquatic and terrestrial, Oslo, Norway.
authors.forename: L K
authors.initials: LK
authors.lastname: Vestby
authors.affiliation: Norwegian Veterinary Institute, Department of Laboratory Services, Section for Bacteriology- aquatic and terrestrial, Oslo, Norway.
authors.forename: K C S
authors.initials: KC
authors.lastname: Johannesen
authors.affiliation: Faculty of Dentistry, Department of Oral Biology, University of Oslo, Oslo, Norway.
authors.forename: I L
authors.initials: IL
authors.lastname: Witsø
authors.affiliation: Nofima, Aas, Norway.
authors.forename: O
authors.initials: O
authors.lastname: Habimana
authors.affiliation: Faculty of Dentistry, Department of Oral Biology, University of Oslo, Oslo, Norway.
authors.forename: A A
authors.initials: AA
authors.lastname: Scheie
authors.affiliation: Norwegian Veterinary Institute, Department of Health Surveillance, Section for Veterinary Public Health, Oslo, Norway.
authors.forename: A M
authors.initials: AM
authors.lastname: Urdahl
authors.affiliation: Faculty of Mathematics and Natural Sciences, Department of Chemistry, University of Oslo, Oslo, Norway.
authors.forename: T
authors.initials: T
authors.lastname: Benneche
authors.affiliation: Nofima, Aas, Norway.
authors.forename: S
authors.initials: S
authors.lastname: Langsrud
authors.affiliation: Norwegian Veterinary Institute, Department of Laboratory Services, Section for Bacteriology- aquatic and terrestrial, Oslo, Norway.
authors.forename: L L
authors.initials: LL
authors.lastname: Nesse
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology; D005407:Flagella; D005525:Food-Processing Industry; D005663:Furans / Q000138:chemical synthesis / Q000494:pharmacology*; D054879:Halogenation; D006801:Humans; D018613:Microscopy, Confocal; D009068:Movement / Q000187:drug effects; D012475:Salmonella / Q000187:drug effects* / Q000502:physiology
chemical_list: C525251:5-bromomethylene-2(5H)-furanone; D000900:Anti-Bacterial Agents; D005663:Furans; D015107:4-Butyrolactone
keywords: E. coli (all potentially pathogenic types); Salmonella; biofilms; food safety