title: An increased number of CD4+CD25+ cells induced by an oral administration of Lactobacillus plantarum NRIC0380 are involved in antiallergic activity.
abstract: BACKGROUND
  Our previous study showed that an oral administration of Lactobacillus plantarum NRIC0380 inhibited immunoglobulin E (IgE) production in a murine model, and that orally administered NRIC0380 induced CD4+CD25+ Foxp3+ T, i.e. regulatory T (Treg), cells in the spleen and Peyer's patch of mice. Although it has been reported that Treg cells might suppress the allergic symptoms, the involvement of the cells in the antiallergic activity of lactic acid bacteria has not been clearly demonstrated. We therefore examined in detail the antiallergic activity of Treg cells obtained from mice that had been fed NRIC0380.
  
  
  METHODS
  Treg cells were obtained from mice that had been fed NRIC0380. The T cell-suppressive effect of the cells was analyzed by coculturing the cells with splenocytes of β-lactoglobulin-immunized mice and β-lactoglobulin. The effects of the Treg cells on the IgE production and cutaneous anaphylaxis reaction were then analyzed by transferring the cells into another mouse.
  
  
  RESULTS
  The Treg cells obtained from the mice that had been fed NRIC0380 showed similar T cell-suppressive activity to those cells obtained from the control mice. The Treg cells obtained from the mice fed NRIC0380 significantly inhibited the IgE production and active cutaneous anaphylaxis reaction when transferred into another mouse that was subsequently immunized with the antigen. Furthermore, the Treg cells also significantly suppressed the passive cutaneous anaphylaxis reaction when cotransferred with the IgE antibody into another mouse.
  
  
  CONCLUSIONS
  The induction of Treg cells by the oral administration of NRIC0380 would be involved in the antiallergic activity of NRIC0380.
authors.affiliation: Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yoshida
authors.forename: Wataru
authors.initials: W
authors.lastname: Fujiwara
authors.forename: Mai
authors.initials: M
authors.lastname: Enomoto
authors.forename: Sayuri
authors.initials: S
authors.lastname: Nakayama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsuda
authors.forename: Hisashi
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Manabu
authors.initials: M
authors.lastname: Shimojoh
authors.forename: Sanae
authors.initials: S
authors.lastname: Okada
authors.forename: Makoto
authors.initials: M
authors.lastname: Hattori
mesh_terms: D000284:Administration, Oral; D000818:Animals; D018926:Anti-Allergic Agents / Q000008:administration & dosage* / Q000276:immunology; D015704:CD4 Antigens / Q000378:metabolism; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010323:Passive Cutaneous Anaphylaxis; D019936:Probiotics / Q000008:administration & dosage*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D018926:Anti-Allergic Agents; D015704:CD4 Antigens; D053645:Interleukin-2 Receptor alpha Subunit; D007073:Immunoglobulin E