title: Isolation by pressurised fluid extraction (PFE) and identification using CPC and HPLC/ESI/MS of phenolic compounds from Brazilian cherry seeds (Eugenia uniflora L.).
abstract: Brazilian cherry seeds are a waste product from juice and frozen pulp production and, the seeds composition was investigated to valorize this by-product. Compounds separation was performed with ethanol by pressurised fluid extraction (PFE). Here we determine the effect of temperature (T), static time (ST), number of cycles (C), and flush volume (VF) on the yield, composition and total phenolic content (TPC) of the seed extracts. T, ST and their interaction positively influenced yield and TPC. Extracts were fractionated by high performance liquid chromatography (HPLC) and centrifugal partition chromatography (CPC). The collected fractions characterizations were made by electrospray ionisation mass spectrometry (ESI/MS) and high resolution mass spectrometry (HRMS) indicated the presence of ellagic acid pentoside and deoxyhexose, quercitrin and kaempferol pentoside. All of these compounds have antioxidant properties and normally are found in plant extracts. These results confirm that Brazilian cherry seed extract is a potentially valuable source of antioxidants.
authors.affiliation: Departamento de Engenharia de Alimentos, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Av. Duque de Caxias Norte, 225, 13635 900 Pirassununga, SP, Brazil. Electronic address: alelopes@usp.br.
authors.forename: Alessandra L
authors.initials: AL
authors.lastname: Oliveira
authors.forename: Emilie
authors.initials: E
authors.lastname: Destandau
authors.forename: Laëtitia
authors.initials: L
authors.lastname: Fougère
authors.forename: Michel
authors.initials: M
authors.lastname: Lafosse
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D001938:Brazil; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry; D011312:Pressure; D027861:Prunus / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D027842:Syzygium
chemical_list: D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts
keywords: Centrifugal partition chromatography; Eugenia uniflora; High performance liquid chromatography; High resolution mass spectrometry; Pressurised fluid extraction