title: Evaluation of the relationship between IL28B, IL10RB and IL28RA single-nucleotide polymorphisms and susceptibility to hepatitis C virus in Chinese Han population.
abstract: Hepatitis C virus (HCV) infection is associated with both viral and host factors. Cytokines, such as interferon (IFN)-λ, play a critical role in modulating the innate and adaptive immune systems. This study aims to investigate the association between single-nucleotide polymorphisms (SNPs) of interleukin (IL) 28B, IL10RB, and IL28RA genes and susceptibility to HCV infection in a population from the Liaoning Province of China. We used high resolution melt-polymerase chain reaction (HRM-PCR) analyses for genotype 6 polymorphisms in these genes in 271 chronic HCV-infected patients and in 300 healthy control subjects. The distribution of IL10RB and IL28RA genotypes among the HCV-infected and control groups did not differ significantly. However, we did find that the four IL28B variants were in complete linkage disequilibrium (r(2) = 0.831-0.922), and the frequency of rs8099917 GT genotype was significantly higher among chronic HCV-infected patients than among controls (OR = 2.21, 95% CI = 1.33-3.68, P = 0.00193); the G allele was found more frequently in the chronic HCV-infected group than in the control group (OR = 2.10, 95% CI = 1.28-3.44, P = 0.00276). Haplotype analysis showed that IL28B (rs12980275, rs11881222, rs12979860 and rs8099917) haplotype AACT had a protective effect for HCV infection (OR = 0.52, 95% CI = 0.33-0.83, P = 0.00551). This study indicates that the four SNPs in IL28B are correlated with susceptibility to HCV infection.
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China. Electronic address: jguojiang@sina.com.cn.
authors.forename: Guojiang
authors.initials: G
authors.lastname: Jin
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China. Electronic address: kanghui65@sina.com.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Kang
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Di
authors.initials: D
authors.lastname: Dai
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics; D002681:China; D005260:Female; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology*; D019698:Hepatitis C, Chronic / Q000235:genetics* / Q000276:immunology / Q000821:virology; D006801:Humans; D007372:Interferons; D053710:Interleukin-10 Receptor beta Subunit / Q000235:genetics*; D007378:Interleukins / Q000235:genetics*; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D018121:Receptors, Cytokine / Q000235:genetics*; D017471:Receptors, Interferon; D012307:Risk Factors; D000095022:Interferon Lambda
chemical_list: D007372:Interferons; D053710:Interleukin-10 Receptor beta Subunit; D007378:Interleukins; D018121:Receptors, Cytokine; D017471:Receptors, Interferon; C000624353:interferon-lambda, human; D000095022:Interferon Lambda; C579640:IFNLR1 protein, human; C081846:IL10RB protein, human
keywords: Hepatitis C virus (HCV); High resolution melt (HRM); IL10RB; IL28B; IL28RA; Polymorphism