title: Effect of salinity on extracellular polymeric substances of activated sludge from an anoxic-aerobic sequencing batch reactor.
abstract: The effect of salinity on extracellular polymeric substances (EPS) of activated sludge was investigated in an anoxic-aerobic sequencing batch reactor (SBR). The contents of loosely bound EPS (LB-EPS) and tightly bound EPS (TB-EPS) were positively correlated with the salinity. The polysaccharide (PS) and protein (PN) contents in both LB-EPS and TB-EPS increased with the increase of salinity. With the increase of salinity from 0.5% to 6%, the PN/PS ratios in LB-EPS and TB-EPS decreased from 4.8 to 0.9 and from 2.9 to 1.4, respectively. The four fluorescence peaks in both LB-EPS and TB-EPS identified by three-dimensional excitation-emission matrix fluorescence spectroscopy are attributed to PN-like substances and humic acid-like substances. The Fourier transform infrared spectra of the LB-EPS and TB-EPS appeared to be very similar, but the differences across the spectra were apparent in terms of the relative intensity of some bands with the increase of salinity. The sludge volume index showed a linear correlation with LB-EPS (R(2)=0.9479) and TB-EPS (R(2)=0.9355) at different salinities, respectively.
authors.affiliation: Key Lab of Marine Environmental Science and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
authors.forename: Zichao
authors.initials: Z
authors.lastname: Wang
authors.forename: Mengchun
authors.initials: M
authors.lastname: Gao
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.forename: Zonglian
authors.initials: Z
authors.lastname: She
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Chang
authors.forename: Changqing
authors.initials: C
authors.lastname: Sun
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Yun
authors.initials: Y
authors.lastname: Ren
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
mesh_terms: D000332:Aerobiosis; D019149:Bioreactors / Q000382:microbiology*; D006812:Humic Substances; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D054712:Salinity; D012722:Sewage / Q000737:chemistry* / Q000382:microbiology; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D006812:Humic Substances; D011135:Polysaccharides, Bacterial; D012722:Sewage
keywords: Extracellular polymeric substances (EPS); Fourier-transform infrared spectroscopy; Salinity; Sludge volume index; Three-dimensional excitation–emission matrix fluorescence spectroscopy