title: Cysteine promoted C-terminal hydrazinolysis of native peptides and proteins.
abstract: Tagging the terminus: N→S acyl transfer in native peptides and proteins can be reliably intercepted with hydrazine. The method allows selective labeling and ligation, without recourse to the use of protein-splicing elements. NCL=native chemical ligation.
authors.affiliation: Department of Chemistry, University College London, 20 Gordon Street, London, WC1H 0AJ (United Kingdom).
authors.forename: Anna L
authors.initials: AL
authors.lastname: Adams
authors.forename: Ben
authors.initials: B
authors.lastname: Cowper
authors.forename: Rachel E
authors.initials: RE
authors.lastname: Morgan
authors.forename: Bhavesh
authors.initials: B
authors.lastname: Premdjee
authors.forename: Stephen
authors.initials: S
authors.lastname: Caddick
authors.forename: Derek
authors.initials: D
authors.lastname: Macmillan
mesh_terms: D000595:Amino Acid Sequence; D003545:Cysteine / Q000737:chemistry*; D004921:Erythropoietin / Q000737:chemistry; D006020:Glycopeptides / Q000737:chemistry; D064451:Hepcidins / Q000737:chemistry; D006801:Humans; D006834:Hydrazines / Q000737:chemistry*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D025801:Ubiquitin / Q000737:chemistry
chemical_list: D006020:Glycopeptides; D064451:Hepcidins; D006834:Hydrazines; D010455:Peptides; D011506:Proteins; D025801:Ubiquitin; D004921:Erythropoietin; D003545:Cysteine
keywords: acyl transfer; erythropoietin; hydrazides; native chemical ligation; peptides