title: Real-time in vivo imaging of invasive- and biomaterial-associated bacterial infections using fluorescently labelled vancomycin.
abstract: Invasive and biomaterial-associated infections in humans are often difficult to diagnose and treat. Here, guided by recent advances in clinically relevant optical imaging technologies, we explore the use of fluorescently labelled vancomycin (vanco-800CW) to specifically target and detect infections caused by Gram-positive bacteria. The application potential of vanco-800CW for real-time in vivo imaging of bacterial infections is assessed in a mouse myositis model and a human post-mortem implant model. We show that vanco-800CW can specifically detect Gram-positive bacterial infections in our mouse myositis model, discriminate bacterial infections from sterile inflammation in vivo and detect biomaterial-associated infections in the lower leg of a human cadaver. We conclude that vanco-800CW has a high potential for enhanced non-invasive diagnosis of infections with Gram-positive bacteria and is a promising candidate for early-phase clinical trials.
authors.affiliation: 1] Department of Medical Microbiology, University of Groningen, University Medical Center Groningen (UMCG), Hanzeplein 1, PO Box 30001, 9700 RB Groningen, The Netherlands [2] Division of Surgical Oncology, Department of Surgery, BioOptical Imaging Center, University of Groningen, UMCG, Hanzeplein 1, PO Box 30001, 9700 RB Groningen, The Netherlands.
authors.forename: Marleen
authors.initials: M
authors.lastname: van Oosten
authors.forename: Tina
authors.initials: T
authors.lastname: Schäfer
authors.forename: Joost A C
authors.initials: JA
authors.lastname: Gazendam
authors.forename: Knut
authors.initials: K
authors.lastname: Ohlsen
authors.forename: Eleni
authors.initials: E
authors.lastname: Tsompanidou
authors.forename: Marcus C
authors.initials: MC
authors.lastname: de Goffau
authors.forename: Hermie J M
authors.initials: HJ
authors.lastname: Harmsen
authors.forename: Lucia M A
authors.initials: LM
authors.lastname: Crane
authors.forename: Ed
authors.initials: E
authors.lastname: Lim
authors.forename: Kevin P
authors.initials: KP
authors.lastname: Francis
authors.forename: Lael
authors.initials: L
authors.lastname: Cheung
authors.forename: Michael
authors.initials: M
authors.lastname: Olive
authors.forename: Vasilis
authors.initials: V
authors.lastname: Ntziachristos
authors.forename: Jan Maarten
authors.initials: JM
authors.lastname: van Dijl
authors.forename: Gooitzen M
authors.initials: GM
authors.lastname: van Dam
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry; D001557:Benzenesulfonates / Q000737:chemistry; D001672:Biocompatible Materials / Q000009:adverse effects; D002102:Cadaver; D003952:Diagnostic Imaging / Q000379:methods*; D004195:Disease Models, Animal; D005456:Fluorescent Dyes / Q000737:chemistry; D006094:Gram-Positive Bacteria / Q000254:growth & development; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D007090:Image Interpretation, Computer-Assisted; D007211:Indoles / Q000737:chemistry; D051379:Mice; D009220:Myositis / Q000175:diagnosis* / Q000382:microbiology; D013997:Time Factors; D014640:Vancomycin / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001557:Benzenesulfonates; D001672:Biocompatible Materials; D005456:Fluorescent Dyes; C562366:IRDye 800CW; D007211:Indoles; D014640:Vancomycin