title: Molecular characterization and phylogenetic analysis of the genome of porcine torovirus.
abstract: In this study, we amplified and sequenced the first genome of porcine torovirus (PToV SH1 strain). The genome was found to be 28,301 bp in length, sharing 79 % identity with Breda virus. It mainly consists of replicase (20,906 bp) and structural genes: spike (4,722 bp), membrane (702 bp), hemagglutinin-esterase (1,284 bp), and nucleocapsid (492 bp). Sequence alignments and structure prediction suggest genetic differences among toroviruses, mainly in NSP1 (papain-like cysteine proteinase domain). Rooted phylogenetic trees were constructed based on the 3C-like proteinase and RNA-dependent RNA polymerase genes. PToV, Berne virus and Breda virus were clustered together, forming a separate branch from white bream virus that was distant from that of the coronaviruses.
authors.forename: Huan
authors.initials: H
authors.lastname: Sun
authors.forename: Daoliang
authors.initials: D
authors.lastname: Lan
authors.forename: Lifang
authors.initials: L
authors.lastname: Lu
authors.forename: Molin
authors.initials: M
authors.lastname: Chen
authors.forename: Changsong
authors.initials: C
authors.lastname: Wang
authors.forename: Xiuguo
authors.initials: X
authors.lastname: Hua
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D017003:Torovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins