title: Isolation and heterotrophic cultivation of mixotrophic microalgae strains for domestic wastewater treatment and lipid production under dark condition.
abstract: Cultivating microalgae heterotrophically could overcome the light dependency, and thus increase the yield of microalgal lipid per unit area. In this study, three newly isolated strains of microalgae (Scenedesmus sp. ZTY2, Scenedesmus sp. ZTY3 and Chlorella sp. ZTY4) from a domestic wastewater treatment plant were heterotrophically cultivated in domestic wastewater with no illumination. During the cultivation, the algal densities of Scenedesmus species and Chlorella species were increased by 203.0% and 60.5% comparing with the initial densities, respectively. After 11-day cultivation, the lipid contents of Scenedesmus sp. ZTY2, Scenedesmus sp. ZTY3 and Chlorella sp. ZTY4 reached 69.1%, 55.3% and 79.2%, respectively. The DOC removal efficiencies of these three strains were 63.4%, 52.9% and 64.4%, and the biomass yield were 1.65, 1.98 and 2.31mg biomass/mg DOC, respectively. This is the first report about the heterotrophic cultivation of microalgae strains for domestic wastewater treatment and lipid production under dark condition.
authors.affiliation: Environmental Simulation and Pollution Control State Key Joint Laboratory, School of Environment, Tsinghua University, Beijing 100084, PR China.
authors.forename: Tian-Yuan
authors.initials: TY
authors.lastname: Zhang
authors.forename: Yin-Hu
authors.initials: YH
authors.lastname: Wu
authors.forename: Shu-feng
authors.initials: SF
authors.lastname: Zhu
authors.forename: Feng-min
authors.initials: FM
authors.lastname: Li
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Hu
mesh_terms: D018533:Biomass; D002244:Carbon / Q000032:analysis; D018929:Cell Culture Techniques / Q000379:methods*; D002708:Chlorella / Q000254:growth & development / Q000302:isolation & purification; D003624:Darkness; D005191:Family Characteristics; D052836:Heterotrophic Processes; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000254:growth & development* / Q000302:isolation & purification*; D044444:Scenedesmus / Q000254:growth & development / Q000302:isolation & purification; D062065:Wastewater / Q000382:microbiology; D018508:Water Purification
chemical_list: D002244:Carbon; D008055:Lipids; D062065:Wastewater
keywords: Heterotrophic cultivation; Lipid production; Microalgal biofuel; Wastewater treatment