title: Comparison of toxicity of different nanorod-type TiO2 polymorphs in vivo and in vitro.
abstract: It is predicted that the toxicity of nanoparticles may be different depending on the properties of the nanoparticles and biological system being tested. However, the factors that influence the toxicity of nanoparticles have not been adequately investigated. In this study, we characterized two types of TiO2 nanorods, anatase (ATO) and brookite (BTO), and compared their toxicity in vivo and in vitro. ATO and BTO differed from each other most notably in their surface areas. Treatment with the two TiO2 nanorods (10 µg ml(-1) ) produced similar effects on the cell cycle in eight cell lines which are derived from potential target organs of nanoparticles, with the BTO eliciting stronger responses than ATO in all cell lines, among the cell lines, H9C2 showed the maximal change. Similarly, when mice were exposed to two TiO2 nanorods (1 mg kg(-1) ), BTO induced clearer histopathological lesions and triggered a more robust secretion of inflammatory cytokines than ATO. Furthermore, we compared the cellular response of both TiO2 nanorods using BEAS-2B cells, the human bronchial epithelial cell line. Both nanorods induced cell death by increasing the formation of autophagosome-like vacuoles. The mitochondrial calcium concentration decreased by exposure of both types, but the distribution of lysosome and endoplasmic reticulum (ER) showed a clear difference between the two nanorods. Thus, we conclude that the surface area acts as an important factor which depends on toxicity of nanorod type-TiO2 nanoparticles. Furthermore, the toxicity of nanoparticles varies according to the type of cells tested, and that the assembly of autophagosome-like vacuoles is a critical part of the cellular response to nanoparticle exposure.
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Suwon, 443-749, Korea.
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Park
authors.forename: Gwang-Hee
authors.initials: GH
authors.lastname: Lee
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Shim
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Myung-Haing
authors.initials: MH
authors.lastname: Cho
authors.forename: Dong-Wan
authors.initials: DW
authors.lastname: Kim
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000276:immunology; D006801:Humans; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D046529:Microscopy, Electron, Transmission; D043942:Nanotubes / Q000737:chemistry*; D010316:Particle Size; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013329:Structure-Activity Relationship; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000633:toxicity*; D018675:Toxicity Tests / Q000379:methods
chemical_list: D016207:Cytokines; D017382:Reactive Oxygen Species; C009495:titanium dioxide; D014025:Titanium
keywords: TiO2; autophagosome; nanorods; surface area; toxicity; vacuoles