title: Effect of concentration of platelet-derived growth factor on proliferative activity of human fibroblasts.
abstract: Serum concentration of platelet-derived growth factor was measured in patients with trophic ulcers and proliferative activity of human fibroblasts was measured in wells with different content of added serum platelet-derived growth factor. In parallel, morphofunctional analysis of platelets from the whole blood of these patients was performed. A close correlation was found between morphofunctional parameters of blood platelets and serum concentration of platelet-derived growth factor. Platelet-derived growth factor in concentrations below 150 pg per well stimulated fibroblast proliferation and preserved their viability. Platelet-derived growth factor in concentrations >200 pg per well suppressed fibroblast proliferation and impaired their viability.
authors.affiliation: N. V. Sklifosovskii Institute of Emergency Medical Care, Moscow, Russia. mcsimmc@yandex.ru.
authors.forename: M S
authors.initials: MS
authors.lastname: Makarov
authors.forename: M V
authors.initials: MV
authors.lastname: Storozheva
authors.forename: O I
authors.initials: OI
authors.lastname: Konyushko
authors.forename: N V
authors.initials: NV
authors.lastname: Borovkova
authors.forename: V B
authors.initials: VB
authors.lastname: Khvatov
mesh_terms: D001792:Blood Platelets / Q000502:physiology; D049109:Cell Proliferation; D048430:Cell Shape; D002470:Cell Survival; D002478:Cells, Cultured; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000502:physiology*; D012883:Skin Ulcer / Q000097:blood
chemical_list: D010982:Platelet-Derived Growth Factor