title: Studies on excretion kinetics of ten constituents in rat urine after oral administration of Shensong Yangxin Capsule by UPLC-MS/MS.
abstract: A rapid and sensitive ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS/MS) method was developed and validated for the quantification of 10 major active constituents in rat urine after oral administration of Shensong Yangxin Capsule (SSYX) using diazepam as an internal standard (IS). The urine samples were pretreated and extracted by solid-phase extraction prior to UPLC. Chromatographic separation was achieved on a Waters C18 (2.1 × 50 mm, 1.7 µm) column using a gradient elution program with 0.1% formic acid aqueous solution and acetonitrile at a flow rate of 0.4 mL/min. Detection and quantitation were accomplished by a hybrid quadrupole mass spectrometer using electrospray ionization source and multiple reaction monitoring in the positive ionization mode. The mass transition ion-pairs (m/z) for quantitation were all optimized and the total run time was 4.50 min. The specificity, linearity, accuracy, precision, recovery, matrix effect and stabilities were all validated for the analytes in urine samples. The validation results indicated that this method was simple, rapid, specific and reliable. The proposed method was successfully applied to investigate the urinary excretion kinetics of 10 compounds in rat after oral administration of SSYX.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Science, Peking University, Beijing, 100191, China; Shijiazhuang Yiling Pharmaceutical Co. Ltd, Shijiazhuang, 050035, People's Republic of China.
authors.forename: Minyan
authors.initials: M
authors.lastname: Liu
authors.forename: Song
authors.initials: S
authors.lastname: Li
authors.forename: Shaohua
authors.initials: S
authors.lastname: Zhao
authors.forename: Hongtao
authors.initials: H
authors.lastname: Wang
authors.forename: Hairong
authors.initials: H
authors.lastname: Wang
authors.forename: Pengfei
authors.initials: P
authors.lastname: Tu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics*; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; C530959:shensongyangxin
keywords: Shensong Yangxin Capsule; UPLC-MS/MS; multiple-reaction monitoring; solid-phase extraction; urinary excretion kinetics