title: Candida albicans adherence and proliferation on the surface of denture base materials.
abstract: OBJECTIVE
  This study aimed to evaluate the adhesion and proliferation of Candida albicans on denture base materials with different resin chemistries.
  
  
  METHODS
  Standardised specimens were prepared from different denture base materials (i.e. Palapress vario, Paladon 65, Castdon, Sherapress, Eclipse and Mucopren) and subjected to thermal cycling to simulate an artificial ageing process. After the determination of surface roughness and surface free energy, Candida albicans adherence and proliferation on the surface of denture base materials were simulated by subsequent incubation with Candida albicans ATCC 10231 for either 24 h or 196 h with and without prior simulation of salivary pellicle formation.
  
  
  RESULTS
  Regardless of the presence of a salivary pellicle or the incubation time, the results indicate that the proliferation of Candida albicans is significantly dependent on the material that is used for the fabrication of denture bases (p < 0.001). A significantly higher proliferation of Candida albicans was observed on the surface of denture base materials with the highest polar contribution to surface free energy (i.e. Eclipse, Mucopren), and this result supports the assumption that there might be a relationship between the polar contribution to surface free energy of the substratum material and Candida albicans proliferation.
  
  
  CONCLUSIONS
  The results obtained from this study indicate that Candida albicans proliferation in particular is not necessarily increased on denture base materials that are subjected to thermal cycling.
authors.affiliation: Department of Prosthetic Dentistry, University Medical Center Regensburg, Regensburg, Germany.
authors.forename: Carola
authors.initials: C
authors.lastname: Koch
authors.forename: Ralf
authors.initials: R
authors.lastname: Bürgers
authors.forename: Sebastian
authors.initials: S
authors.lastname: Hahnel
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D018441:Biofilms / Q000254:growth & development; D002176:Candida albicans / Q000254:growth & development / Q000502:physiology*; D003188:Composite Resins / Q000737:chemistry; D003764:Dental Materials / Q000737:chemistry*; D044622:Dental Pellicle / Q000382:microbiology; D003819:Denture Bases / Q000382:microbiology*; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D008768:Methylmethacrylates / Q000737:chemistry; D012833:Siloxanes / Q000737:chemistry; D013499:Surface Properties; D013500:Surface Tension; D013997:Time Factors
chemical_list: D000180:Acrylic Resins; D003188:Composite Resins; D003764:Dental Materials; C509237:Eclipse Prosthodontic Resin; D008689:Methacrylates; D008768:Methylmethacrylates; C507521:Palapress Vario; D012833:Siloxanes; C024149:paladon
keywords: Candida albicans; biofilm; denture; stomatitis