title: Improved staining of phosphoproteins with high sensitivity in polyacrylamide gels using Stains-All.
abstract: An improved Stains-All (ISA) staining method for phosphoproteins in SDS-PAGE was described. Down to 0.5-1 ng phosphoproteins (α-casein, β-casein, or phosvitin) can be successfully selectively detected by ISA stain, which is approximately 120-fold higher than that of original Stains-All stain, but is similar to that of commonly used Pro-Q Diamond stain. Furthermore, unlike the original Stains-All protocol that was time consuming and light unstable, ISA stain could be completed within 60 min without resorting to protect the gels from light during the whole staining procedure. According to the results, it is concluded that ISA stain is a rapid, sensitive, specific, and economic staining method for a broad application to the research of phosphoproteins.
authors.affiliation: Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College, Wenzhou, Zhejiang, P. R. China; Wenzhou Undersun Biotechnology Co., Ltd, Wenzhou, Zhejiang, P. R. China.
authors.forename: Wei-Tao
authors.initials: WT
authors.lastname: Cong
authors.forename: Wei-Jian
authors.initials: WJ
authors.lastname: Ye
authors.forename: Mao
authors.initials: M
authors.lastname: Chen
authors.forename: Ting
authors.initials: T
authors.lastname: Zhao
authors.forename: Zhong-Xin
authors.initials: ZX
authors.lastname: Zhu
authors.forename: Chao
authors.initials: C
authors.lastname: Niu
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Ruan
authors.forename: Mao-Wei
authors.initials: MW
authors.lastname: Ni
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.forename: Li-Tai
authors.initials: LT
authors.lastname: Jin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002232:Carbocyanines / Q000737:chemistry*; D002364:Caseins / Q000032:analysis / Q000737:chemistry; D004396:Coloring Agents / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D013194:Staining and Labeling
chemical_list: D002232:Carbocyanines; D002364:Caseins; D004396:Coloring Agents; D010750:Phosphoproteins; C012040:stains-all
keywords: Phosphoprotein; Pro-Q diamond; SDS-PAGE; Stain; Stains-All