title: Sporadic colon cancer murine models demonstrate the value of autoantibody detection for preclinical cancer diagnosis.
abstract: Although autoantibody detection has been proposed for diagnosis of colorectal cancer, little is known about their initial production and development correlation with cancer progression. Azoxymethane/dextran sodium sulfate (AOM/DSS)-treated mice developed colon adenocarcinoma in the distal colon similar to human sporadic colon cancer. We assessed this model together with AOM and DSS-only models for their applicability to early detection of cancer. All AOM/DSS-treated mice produced autoantibodies to tumor-associated antigens analogous to those observed in human colon cancer patients. Autoantibody response was related to tumor antigen overexpression. Cancer autoantibodies were detected 21 days after starting treatment, when no malignant histopathological features were detectable, and they increased according to tumor progression. When carcinogenesis was induced separately by AOM or DSS, only those mice that developed malignant lesions produced significant levels of autoantibodies. These findings demonstrate that autoantibody development is an early event in tumorigenesis and validates its use for preclinical colon cancer diagnosis.
authors.affiliation: 1] Department of Cellular and Molecular Medicine. Centro de Investigaciones Biológicas (CSIC). 28040 Madrid. Spain [2] [3].
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Barderas
authors.forename: Roi
authors.initials: R
authors.lastname: Villar-Vázquez
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Fernández-Aceñero
authors.forename: Ingrid
authors.initials: I
authors.lastname: Babel
authors.forename: Alberto
authors.initials: A
authors.lastname: Peláez-García
authors.forename: Sofía
authors.initials: S
authors.lastname: Torres
authors.forename: J Ignacio
authors.initials: JI
authors.lastname: Casal
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000276:immunology / Q000473:pathology; D003092:Colitis / Q000139:chemically induced / Q000150:complications; D003110:Colonic Neoplasms / Q000175:diagnosis* / Q000209:etiology / Q000473:pathology; D004195:Disease Models, Animal; D055088:Early Detection of Cancer; D015870:Gene Expression; D006801:Humans; D051379:Mice
chemical_list: D000951:Antigens, Neoplasm; D001323:Autoantibodies