title: Performance evaluation of commercial ELISA kits for screening of furazolidone and furaltadone residues in fish.
abstract: Regulatory monitoring for nitrofuran drug residues in aquaculture products has largely focused on LC-MS/MS. In addition, there is a need for facile and high-throughput screening methods for monitoring programs. We evaluated the performance of Ridascreen (R-Biopharm) ELISA kits for nitrofuran drug residues in fish muscle, with verification by LC-MS/MS. Kits were available for 3-amino-2-oxazolidinone (AOZ) and 3-amino-5-morpholino-methyl-2-oxazolidinone (AMOZ) side-chains of furazolidone and furaltadone, respectively. We found good repeatability in fortified and incurred muscle samples, with RSDs ranging from 1.8% to 7.6%. Recoveries of AOZ and AMOZ from muscle fortified at levels of 0.5-2 ng/g ranged from 98% to 114%. Excellent selectivity was demonstrated. The minimum detection limits (MDLs) for AOZ and AMOZ in muscle were 0.05 and 0.2 ng/g, respectively. ELISA data were highly correlated with those of LC-MS/MS. Results of this study support the use of these kits as screening assays for nitrofuran residues in fish muscle.
authors.affiliation: FDA, Division of Seafood Science and Technology, Gulf Coast Seafood Laboratory, Dauphin Island, AL 36528, United States. Electronic address: Edward.Jester@fda.hhs.gov.
authors.forename: Edward L E
authors.initials: EL
authors.lastname: Jester
authors.forename: Ann
authors.initials: A
authors.lastname: Abraham
authors.forename: Yuesong
authors.initials: Y
authors.lastname: Wang
authors.forename: Kathleen R
authors.initials: KR
authors.lastname: El Said
authors.forename: Steven M
authors.initials: SM
authors.lastname: Plakas
mesh_terms: D000818:Animals; D017756:Aquaculture; D002138:Calibration; D002853:Chromatography, Liquid; D004350:Drug Residues / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005664:Furazolidone / Q000032:analysis*; D057166:High-Throughput Screening Assays; D007059:Ictaluridae; D009025:Morpholines / Q000737:chemistry; D009581:Nitrofurans / Q000032:analysis*; D023303:Oxazolidinones / Q000032:analysis* / Q000737:chemistry; D017747:Seafood / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: C504349:3-amino-5-morpholinomethyl-2-oxazolidinone; D009025:Morpholines; D009581:Nitrofurans; D023303:Oxazolidinones; D005664:Furazolidone; C007795:furaltadon; C000209:3-amino-2-oxazolidinone
keywords: ELISA; Furaltadone; Furazolidone