title: [Gene expression of bone mesenchymal stem cells transduced by the lentiviral vector of SOX9 gene].
abstract: OBJECTIVE
  To construct one lentiviral vector containing mouse SRY-related high mobility group-box gene 9 (SOX9) and transfect the murine bone mesenchymal stem cells (mBMSCs) in vitro and observe the expression of target gene.
  
  
  METHODS
  RNA from the vectors containing mouse SOX9 gene were extracted and SOX9 genes were amplified by reverse transcription-Polymerase Chain Reaction (RT-PCR). The SOX9 genes were connected into lentiviral vectors pGC-FU. Then pGC-FU-SOX9 transduced into 293T cells to produce recombinant lentivirus called as Lenti-SOX9-EGFP. mBMSCs were transfected. The expression of target gene was detected by immunofluorescence, RT-PCR and Western Blot.
  
  
  RESULTS
  Lenti-SOX9-EGFP was recombined successfully and transduced efficiently into mBMSCs. The expression of SOX9 gene was confirmed by RT-PCR and Western Blot.
  
  
  CONCLUSIONS
  Lentiviral vector of mouse SOX9 gene can transfect successfully into mBMSCs. Meanwhile, SOX9 gene may be expressed in mBMSCs. This will provide the target cells for the following study about SOX9 gene repairing cartilage injury.
authors.affiliation: Department of Orthopaedics, the Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi, China.
authors.forename: Jie-yu
authors.initials: JY
authors.lastname: Bai
authors.forename: Da-chuan
authors.initials: DC
authors.lastname: Liang
authors.forename: Peng
authors.initials: P
authors.lastname: Cheng
authors.forename: Zi-quan
authors.initials: ZQ
authors.lastname: Yang
authors.forename: Xiao-chun
authors.initials: XC
authors.lastname: Wei
mesh_terms: D000818:Animals; D005260:Female; D015870:Gene Expression; D015316:Genetic Therapy; D005822:Genetic Vectors; D016086:Lentivirus / Q000235:genetics; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D010003:Osteoarthritis / Q000628:therapy*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055758:SOX9 Transcription Factor / Q000235:genetics*; D014161:Transduction, Genetic; D014162:Transfection
chemical_list: D055758:SOX9 Transcription Factor; C527933:Sox9 protein, mouse