title: The use of chromatin immunoprecipitation (ChIP) to study the binding of viral proteins to the adenovirus genome in vivo.
abstract: The encapsidation of adenovirus (Ad) DNA into virus particles depends on cis-acting sequences located at the left end of the viral genome. Repeated DNA sequences in the packaging domain contribute to viral DNA encapsidation and several viral proteins bind to these repeats when analyzed using in vitro DNA-protein binding assays. In this chapter, we describe a chromatin immunoprecipitation (ChIP) approach to study the binding of viral proteins to packaging sequences in vivo. This assay permits accurate quantification over a wide range of DNA concentrations. The use of formaldehyde cross-linking to stabilize DNA-protein and protein-protein complexes formed in vivo allows the identification of macromolecular complexes found in living cells.
authors.affiliation: Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, NY, USA.
authors.forename: Yueting
authors.initials: Y
authors.lastname: Zheng
authors.forename: Patrick
authors.initials: P
authors.lastname: Hearing
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D016679:Genome, Viral; D006801:Humans; D011485:Protein Binding; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D014764:Viral Proteins