title: 59Fe-distribution in conditional ferritin-H-deleted mice.
abstract: The objective was to explore how ferritin-H deletion influences (59)Fe-distribution and excretion-kinetics in mice. Kinetics of (59)Fe-release from organs, whole-body excretion, and distribution-kinetics of intravenously injected (59)Fe trace amounts were compared in iron-deficient and iron-replete mice with (Fth(Δ/Δ)) and without (Fth(lox/lox)) conditional Mx-Cre-induced ferritin-H deletion. (59)Fe was released from spleen and liver beginning on day 2 and day 5 after ferritin-H deletion, respectively, but was not excreted from the body. Plasma-(59)Fe was cleared significantly faster in iron-deficient Fth(Δ/Δ)-mice than in iron-adequate Fth(lox/lox)-controls. (59)Fe-distribution showed a transient peak (e.g., in heart, kidney, muscle) in Fth(lox/lox) control mice, but not in ferritin-H-deleted Fth(Δ/Δ) mice 24 hours after (59)Fe injection. (59)Fe uptake into the liver and spleen was significantly lower in iron-deficient Fth(Δ/Δ) than in Fth(lox/lox) mice 24 hours and 7 days after injection, respectively, and rapidly appeared in circulating erythrocytes instead. The rate of (59)Fe release after ferritin-H deletion supports earlier data on ferritin turnover in mammals; released (59)Fe is not excreted from the body. Instead, (59)Fe is channeled into erythropoiesis and circulating erythrocytes significantly more extensively and faster. Along with a lack of transient interim (59)Fe storage (e.g., in the heart and kidney), this finding is evidence for ferritin-related iron storage-capacity affecting rate and extent of iron utilization.
authors.affiliation: Molecular Nutrition Unit, Research Center for Nutrition and Food Science, Technische Universität München, Freising-Weihenstephan, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Kistler
authors.affiliation: Molecular Nutrition Unit, Research Center for Nutrition and Food Science, Technische Universität München, Freising-Weihenstephan, Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Even
authors.affiliation: Chair for Biofunctionality, Research Center for Nutrition and Food Science, Center for Diet and Disease, Technische Universität München, Freising-Weihenstephan, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Wagner
authors.affiliation: Institut für Tierernährung der TUM, Freising-Weihenstephan, Germany.
authors.forename: Christiane
authors.initials: C
authors.lastname: Becker
authors.affiliation: Queensland Institute of Medical Research, PO Royal Brisbane Hospital, Brisbane, Australia.
authors.forename: Depak
authors.initials: D
authors.lastname: Darshan
authors.affiliation: Ecole Polytechnique Fédérale de Lausanne, Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland.
authors.forename: Liviu
authors.initials: L
authors.lastname: Vanoaica
authors.affiliation: Ecole Polytechnique Fédérale de Lausanne, Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland.
authors.forename: Lucas C
authors.initials: LC
authors.lastname: Kühn
authors.affiliation: Molecular Nutrition Unit, Research Center for Nutrition and Food Science, Technische Universität München, Freising-Weihenstephan, Germany. Electronic address: kschuemann@hgrunowfoundation.org.
authors.forename: Klaus
authors.initials: K
authors.lastname: Schümann
mesh_terms: D000818:Animals; D001052:Apoferritins / Q000502:physiology*; D007501:Iron / Q000378:metabolism; D007504:Iron Radioisotopes / Q000493:pharmacokinetics*; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D007504:Iron Radioisotopes; D001052:Apoferritins; D007501:Iron