title: Distinct human T-lymphocyte responses triggered by Porphyromonas gingivalis capsular serotypes.
abstract: OBJECTIVE
  Porphyromonas gingivalis can synthesize an extracellular capsule and different serotypes have been described based on capsular antigenicity. On dendritic cells (DCs), the type of capsule present plays a role on the strength of the developed immune response. This study aimed to investigate the T-lymphocyte responses when stimulated with autologous mature DCs exposed to different P. gingivalis K-serotypes.
  
  
  METHODS
  Naïve CD4(+) T-lymphocytes were obtained from healthy subjects and stimulated with autologous DCs primed with increasing multiplicity of infections of the different P. gingivalis K-serotypes. The Th1, Th2, Th17 and T-regulatory cytokines and transcription factor levels were quantified.
  
  
  RESULTS
  Distinct types of response were detected when T-lymphocytes were stimulated by DCs primed with the different P. gingivalis K-serotypes. T-lymphocytes stimulated by K1 or K2-primed DCs elicited higher levels of Th1 and Th17-associated cytokines, T-bet and RORC2 than T-lymphocytes stimulated with DCs primed with the other serotypes. Conversely, the serotypes K3-K5 induced higher levels of Th2-associated cytokines and GATA-3 than the others.
  
  
  CONCLUSIONS
  These results demonstrate that DCs primed with the different P. gingivalis K-serotypes elicited distinct T-cell responses. Strains K1 (W83) and K2 (HG184) induced a Th1/Th17 pattern of immune response and K3 (A7A1-28), K4 (ATCC(®49417™) ), and K5 (HG1690) a Th2 response.
authors.affiliation: Department of Cellular and Molecular Medicine, Centro de Investigaciones Biológicas (CIB-CSIC), Madrid, Spain; Periodontal Biology Laboratory, Department of Conservative Dentistry, Dental School, Universidad de Chile, Santiago de Chile, Chile.
authors.forename: Rolando
authors.initials: R
authors.lastname: Vernal
authors.forename: Eva
authors.initials: E
authors.lastname: Diaz-Guerra
authors.forename: Augusto
authors.initials: A
authors.lastname: Silva
authors.forename: Mariano
authors.initials: M
authors.lastname: Sanz
authors.forename: Jose A
authors.initials: JA
authors.lastname: Garcia-Sanz
mesh_terms: D016667:Bacterial Capsules / Q000276:immunology*; D001431:Bacteriological Techniques; D016207:Cytokines / Q000032:analysis; D003713:Dendritic Cells / Q000276:immunology / Q000382:microbiology*; D051858:Forkhead Transcription Factors / Q000032:analysis; D050990:GATA3 Transcription Factor / Q000032:analysis; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis; D007378:Interleukins / Q000032:analysis; D008213:Lymphocyte Activation / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3 / Q000032:analysis; D016966:Porphyromonas gingivalis / Q000145:classification / Q000276:immunology*; D012703:Serotyping; D020825:T-Box Domain Proteins / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology* / Q000382:microbiology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D058504:Th17 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D053773:Transforming Growth Factor beta1 / Q000032:analysis; D048069:Tumor Necrosis Factors / Q000032:analysis; D000098815:T-bet Transcription Factor
chemical_list: D016207:Cytokines; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit; D007378:Interleukins; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; C539900:RORC protein, human; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D053773:Transforming Growth Factor beta1; D048069:Tumor Necrosis Factors; D007371:Interferon-gamma
keywords: Porphyromonas gingivalis; T helper lymphocytes; cytokines; serotypes; transcription factors