title: [Pulmonary expression of Nr1d1 in rats with ventilation-induced lung injury].
abstract: OBJECTIVE
  To investigate the changes in the expression of nuclear receptor subfamily1 (Nr1d1) in the lungs of rats with ventilation-induced lung injury and explore the molecular mechanism.
  
  
  METHODS
  Seventy-two rats were randomly divided into 4 groups for observation at 4 time points (1:00 AM, 7:00 AM, 1:00 PM and 7:00 PM), and each group was further divided into 3 subgroups (Group FB, Group LV and Group HV). After ventilation for 3 h, the rats were sacrificed and lung tissue samples were obtained for testing the expression of Nr1d1 using gene chip technology and RT-PCR.
  
  
  RESULTS
  Nr1d1 gene expression was down-regulated in HV group as shown by gene chip RT-PCR data. Cosine analysis showed that Nr1d1 expression exhibited a rhythmicity in FB group (P<0.05).
  
  
  CONCLUSIONS
  The rhythmicity of Nr1d1 expression disappears as the tidal volume the increase. Diminished Nr1d1 expression is closely correlated with ventilation-induced lung injury in rats.
authors.affiliation: Department of Anesthesiology, Guangdong Academy of Medical Sciences, Guangdong General Hospital, Guangzhou 510080, China. E-mail: jiajiashijiajia1@sina.com.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Hu
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Wang
mesh_terms: D000818:Animals; D002940:Circadian Rhythm; D015536:Down-Regulation; D005260:Female; D008168:Lung / Q000378:metabolism; D008297:Male; D056953:Nuclear Receptor Subfamily 1, Group D, Member 1 / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012121:Respiration, Artificial / Q000009:adverse effects / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013990:Tidal Volume; D055397:Ventilator-Induced Lung Injury / Q000378:metabolism*
chemical_list: C498263:Nr1d1 protein, rat; D056953:Nuclear Receptor Subfamily 1, Group D, Member 1