title: Hymenobacter swuensis sp. nov., a gamma-radiation-resistant bacteria isolated from mountain soil.
abstract: Gram stain-negative and non-motile bacteria, designated as DY53(T) and DY43, were isolated from mountain soil in South Korea prior exposure with 5 kGy gamma radiation. Phylogenetic analysis based on 16S rRNA gene sequence revealed that the strains belonged to the family Cytophagaceae in the class Cytophagia. 16S rRNA gene sequence similarity of strains DY53(T) and DY43 was 100 %. The highest degrees of sequence similarities of strains DY53(T) and DY43 were found with Hymenobacter perfusus A1-12(T) (98.8 %), Hymenobacter rigui WPCB131(T) (98.5 %), H. yonginensis HMD1010(T) (97.9 %), H. xinjiangensis X2-1g(T) (96.6 %), and H. gelipurpurascens Txg1(T) (96.5 %). The DNA G+C content of the novel strains DY53(T) and DY43 were 59.5 mol%. Chemotaxonomic data revealed that strains possessed major fatty acids such as C₁₅:₀ iso, C₁₅:₀ anteiso, C₁₆:₁ ω5c, summed feature 3 (16:1 ω7c/ω6c), summed feature 4 (17:1 anteiso B/iso I) and C₁₇:₀ iso, and major polar lipid was phosphatidylethanolamine. The novel strains showed resistance to gamma radiation, with a D10 value (i.e., the dose required to reduce the bacterial population by tenfold) in excess of 5 kGy. Based on these data, strains DY53(T) and DY43 should be classified as representing a novel species, for which the name Hymenobacter swuensis sp. nov. is proposed, with the type strain DY53(T) (=KCTC 32018(T) = JCM 18582(T)) and DY43 (=KCTC 32010).
authors.affiliation: Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 139-774, Republic of Korea.
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Lee
authors.forename: Sathiyaraj
authors.initials: S
authors.lastname: Srinivasan
authors.forename: Sangyong
authors.initials: S
authors.lastname: Lim
authors.forename: Minho
authors.initials: M
authors.lastname: Joe
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Shin Ae
authors.initials: SA
authors.lastname: Kwon
authors.forename: Yoon Jung
authors.initials: YJ
authors.lastname: Kwon
authors.forename: Jin
authors.initials: J
authors.lastname: Lee
authors.forename: Jin Ju
authors.initials: JJ
authors.lastname: Choi
authors.forename: Hye Min
authors.initials: HM
authors.lastname: Lee
authors.forename: Young Kyung
authors.initials: YK
authors.lastname: Auh
authors.forename: Myung Kyum
authors.initials: MK
authors.lastname: Kim
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000528:radiation effects; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005720:Gamma Rays; D007723:Korea; D050296:Microbial Viability / Q000528:radiation effects; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S