title: Expression of neuronal protein Kidins220/ARMS in the spleen and peripheral blood of mice following airway allergen challenge.
abstract: Nerve growth factor (NGF), combined with the high-affinity tyrosine kinase receptor A (TrkA), has been reported to be involved in the pathogenesis of asthma. Ankyrin-rich membrane spanning/transmembrane substrate of protein kinase D (ARMS/Kidins220), a TrkA‑binding protein, modulates the NGF signaling pathway. The aim of the present study was to investigate the expression of Kidins220/ARMS and the effect NGF has on the protein in the spleen and peripheral blood, following airway allergen challenge in mice. BALB/c mice were sensitized and challenged with ovalbumin. The effects of NGF on Kidins220/ARMS in the spleen and peripheral blood of mice were assessed by administering anti-NGF antibody. Expression of ARMS, interleukin (IL)-1β and IL-4 in the spleen and peripheral blood was observed by reverse transcription-polymerase chain reaction, western blot analysis and immunohistochemistry. Pathological changes in the bronchi and lung tissues were examined by hematoxylin and eosin staining. Results showed that Kidins220/ARMS, IL-1β and IL-4 were overexpressed in the spleen and peripheral blood following allergen challenge, compared with the control mice. Moreover, following treatment with anti-NGF, the levels of Kidins220/ARMS, IL-1β and IL-4 in the mice were downregulated. Therefore, the results of the present study showed that Kidins220/ARMS is expressed in the spleen and peripheral blood of normal BALB/c mice and may participate in the immuno-inflammation of asthma through the NGF-mediated signaling pathway.
authors.affiliation: Liaoning University of Tradional Chinese Medicine, Shenyang, Liaoning 116600, P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Dong
authors.forename: Chang
authors.initials: C
authors.lastname: Yang
authors.forename: Yuli
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Ni
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D015153:Blotting, Western; D005260:Female; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008565:Membrane Proteins / Q000097:blood* / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000378:metabolism*; D010047:Ovalbumin / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012137:Respiratory System / Q000276:immunology*; D013154:Spleen / Q000378:metabolism*
chemical_list: D000485:Allergens; D053583:Interleukin-1beta; C544017:Kidins220 protein, mouse; D008565:Membrane Proteins; D012333:RNA, Messenger; D015847:Interleukin-4; D010047:Ovalbumin