title: Lettuce necrotic leaf curl virus, a new plant virus infecting lettuce and a proposed member of the genus Torradovirus.
abstract: A new virus was isolated from a lettuce plant grown in an open field in the Netherlands in 2011. This plant was showing conspicuous symptoms that consisted of necrosis and moderate leaf curling. The virus was mechanically transferred to indicator plants, and a total RNA extract of one of these indicator plants was used for next-generation sequencing. Analysis of the sequences that were obtained and further biological studies showed that the virus was related to, but clearly distinct from, viruses belonging to the genus Torradovirus. The name "lettuce necrotic leaf curl virus" (LNLCV) is proposed for this new torradovirus.
authors.affiliation: Plant Research International, Part of Wageningen UR (University and Research Centre), P.O. Box 69, 6700 AB, Wageningen, The Netherlands, martin.verbeek@wur.nl.
authors.forename: Martin
authors.initials: M
authors.lastname: Verbeek
authors.forename: Annette M
authors.initials: AM
authors.lastname: Dullemans
authors.forename: Henry M G
authors.initials: HM
authors.lastname: van Raaij
authors.forename: Jacobus Th J
authors.initials: JT
authors.lastname: Verhoeven
authors.forename: René A A
authors.initials: RA
authors.lastname: van der Vlugt
mesh_terms: D016000:Cluster Analysis; D016679:Genome, Viral; D018545:Lactuca / Q000821:virology*; D008969:Molecular Sequence Data; D009426:Netherlands; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D012367:RNA, Viral