title: A polysaccharide isolated fromAgaricus blazeiMurill (ABP-AW1) as a potential Th1 immunity-stimulating adjuvant.
abstract: In the present study, a low molecular weight polysaccharide, ABP-AW1, isolated fromAgaricus blazeiMurill was assessed for its potential adjuvant activity. ABP-AW1 is considered to create a 'depot' of antigen at a subcutaneous injection site. ICR mice were immunized with 100 μg ovalbumin (OVA) alone or with 100 μg OVA formulated in 0.9% saline containing 200 μg aluminum (alum) or ABP-AW1 (50, 100 and 200 μg) on days 1 and 15. Two weeks after the secondary immunization, splenocyte proliferation, the expression of surface markers, cytokine production and the OVA-specific antibody levels in the serum were determined. The OVA/ABP-AW1 vaccine, in comparison with OVA alone, markedly increased the proliferation of splenic lymphocytes and elicited greater antigen-specific CD4+T cell activation, as determined by splenic CD4+CD69+T cells and Th1 cytokine interferon (IFN)-γ release. The combination of ABP-AW1 and OVA also enhanced IgG2b antibody responses to OVA. In conclusion, these data indicated that ABP-AW1 significantly enhanced the humoral and cellular immune responses against OVA in the mice, suggesting that ABP-AW1 stimulated Th1-type immunity. We suggest that ABP-AW1 may serve as a new adjuvant.
authors.affiliation: Department of Medicine Research, The First Affliated Hospital, Qiqihar Medical University, Qiqihar, Heilongjiang 161041, P.R. China ; Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, P.R. China.
authors.forename: Liran
authors.initials: L
authors.lastname: Cui
authors.forename: Yongxu
authors.initials: Y
authors.lastname: Sun
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.forename: Huiyu
authors.initials: H
authors.lastname: Xu
authors.forename: Huan
authors.initials: H
authors.lastname: Cong
authors.forename: Jicheng
authors.initials: J
authors.lastname: Liu
keywords: Agaricus blazei; Th1; immune; polysaccharide; vaccine