title: Resonance light scattering determination of trace bisphenol A with signal amplification by aptamer-nanogold catalysis.
abstract: HAuCl4 was reduced by sodium citrate to prepare 10 nm gold nanoparticles (AuNPs) that were modified by the bisphenol A aptamer (Apt) to obtain an aptamer-nanogold probe (Apt-AuNP) for bisphenol A (BPA). The probes were aggregated nonspecifically to form large clusters, which showed a strong resonance light scattering (RLS) peak at 520 nm, under preparation conditions (pH 7.6 Na2HPO4-NaH2PO4 buffer and ultrasonication). Upon addition of BPA, the probe reacted specifically to form dispersed BPA-Apt-AuNP conjugates that exhibited strong catalysis of the two particle reactions of glucose-Cu(II) and hydrazine hydrochloride-Cu(II) with a strong RLS peak at 360 nm and 510 nm respectively. When the BPA concentration increased, the RLS intensity at 360 nm and 510 nm increased respectively. Accordingly, two new and highly-sensitive RLS methods were established for the detection of BPA, using the Apt-AuNP catalytic amplification.
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, Guilin, 541004, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Yao
authors.forename: Aihui
authors.initials: A
authors.lastname: Liang
authors.forename: Wenqing
authors.initials: W
authors.lastname: Yin
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001559:Benzhydryl Compounds / Q000737:chemistry*; D002384:Catalysis; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*; D000098826:Bisphenol A Compounds
chemical_list: D052157:Aptamers, Nucleotide; D001559:Benzhydryl Compounds; D006046:Gold; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Fehling reagent; aptamer, nanocatalysis; bisphenol A; resonance light scattering