title: Adherence ability of Staphylococcus epidermidis on prosthetic biomaterials: an in vitro study.
abstract: Bacterial adhesion to the surface of biomaterials is an essential step in the pathogenesis of implant-related infections. In this in vitro research, we evaluated the ability of Staphylococcus epidermidis to adhere to the surface of solid biomaterials, including oxidized zirconium-niobium alloy (Oxinium), cobalt-chromium-molybdenum alloy, titanium alloy, commercially pure titanium, and stainless steel, and performed a biomaterial-to-biomaterial comparison. The test specimens were physically analyzed to quantitatively determine the viable adherent density of the S. epidermidis strain RP62A (American Type Culture Collection [ATCC] 35984). Field emission scanning electron microscope and laser microscope examination revealed a featureless, smooth surface in all specimens (average roughness <10 nm). The amounts of S. epidermidis that adhered to the biomaterial were significantly lower for Oxinium and the cobalt-chromium-molybdenum alloy than for commercially pure titanium. These results suggest that Oxinium and cobalt-chromium-molybdenum alloy are less susceptible to bacterial adherence and are less inclined to infection than other materials of a similar degree of smoothness.
authors.affiliation: Department of Orthopedic Surgery, Graduate School of Medicine, Nagasaki University, Nagasaki, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Shida
authors.forename: Hironobu
authors.initials: H
authors.lastname: Koseki
authors.forename: Itaru
authors.initials: I
authors.lastname: Yoda
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Horiuchi
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Sakoda
authors.forename: Makoto
authors.initials: M
authors.lastname: Osaki
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D004866:Equipment Contamination / Q000517:prevention & control*; D008422:Materials Testing; D008670:Metals / Q000737:chemistry / Q000494:pharmacology*; D019736:Prostheses and Implants / Q000382:microbiology*; D013212:Staphylococcus epidermidis / Q000166:cytology* / Q000502:physiology*
chemical_list: D001672:Biocompatible Materials; D008670:Metals
keywords: bacterial adhesion; implant; infection; surface character