title: [Establishment of full-sib families of Branchiostoma japonicum and the relationship between early development patterns and larvae survival rates].
abstract: One general requirement of individual laboratory animals is that they have known genetic backgrounds. However, ensuring such genetic similarity is difficult, and can be facilitated by breeding a full strain for experimentation. To this end, the authors bred 34 full-sib families of amphioxus larvae/embryos. Due to the high mortality of the embryos and larvae, only seven full-sib families of juvenile amphioxus Branchiostoma japonicum were obtained. Among them, the highest and lowest survival ratios were 32.4% and 1.67%, respectively, whereas the shortest metamorphosis and longest larva duration were 24 d and 42 d, respectively. These results demonstrate the feasibility of establishing full-sib families of amphioxus, and provide fundamental data needed for the future breeding of amphioxus strains.
authors.affiliation: School of Life Sciences, Xiamen University, Xiamen 361005, China. wangyq@xmu.edu.cn.
authors.forename: Wei-Ye
authors.initials: WY
authors.lastname: Li
authors.forename: Yi-Quan
authors.initials: YQ
authors.lastname: Wang
mesh_terms: D000818:Animals; D001947:Breeding; D005260:Female; D005306:Fertilization; D064175:Lancelets / Q000145:classification / Q000196:embryology* / Q000235:genetics / Q000502:physiology; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D008297:Male; D015996:Survival Rate
keywords: Amphioxus; Development; Full-sib family; Propagation; Survival ratio