title: Synthesis and protective effect of new ligustrazine-benzoic acid derivatives against CoCl2-induced neurotoxicity in differentiated PC12 cells.
abstract: A series of novel ligustrazine-benzoic acid derivatives were synthesized and evaluated for their protective effect against cobalt chloride-induced neurotoxicity in differentiated PC12 cells. Combining hematoxylin and eosin staining, we found compound that (3,5,6-trimethylpyrazin-2-yl)methyl 3-methoxy-4-[(3,5,6-trimethylpyrazin-2-yl)methoxy]benzoate (4a) displayed promising protective effect on the proliferation of the injured PC12 cells (EC50 = 4.249 µM). Structure-activity relationships are brieﬂy discussed.
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. zyz100102@126.com.
authors.forename: Penglong
authors.initials: P
authors.lastname: Wang
authors.forename: Honggui
authors.initials: H
authors.lastname: Zhang
authors.forename: Fuhao
authors.initials: F
authors.lastname: Chu
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.forename: Jinxuan
authors.initials: J
authors.lastname: Lin
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Chen
authors.forename: Guoliang
authors.initials: G
authors.lastname: Li
authors.forename: Yatao
authors.initials: Y
authors.lastname: Cheng
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Yuzhong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Haimin
authors.initials: H
authors.lastname: Lei
mesh_terms: D000818:Animals; D001565:Benzoates / Q000138:chemical synthesis* / Q000494:pharmacology; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects; D003035:Cobalt / Q000633:toxicity; D020128:Inhibitory Concentration 50; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D018696:Neuroprotective Agents / Q000138:chemical synthesis / Q000494:pharmacology; D016716:PC12 Cells; D011719:Pyrazines / Q000737:chemistry*; D051381:Rats
chemical_list: D001565:Benzoates; D018696:Neuroprotective Agents; D011719:Pyrazines; D003035:Cobalt; C018021:cobaltous chloride; C017953:tetramethylpyrazine