title: A mediator-free glucose biosensor based on glucose oxidase/chitosan/α-zirconium phosphate ternary biocomposite.
abstract: A novel glucose oxidase/chitosan/α-zirconium phosphate (GOD/chitosan/α-ZrP) ternary biocomposite was prepared by co-intercalating glucose oxidase (GOD) and chitosan into the interlayers of α-zirconium phosphate (α-ZrP) via a delamination-reassembly procedure. The results of X-ray diffraction, infrared spectroscopy, circular dichroism, and ultraviolet spectrum characterizations indicated not only the layered and hybrid structure of the GOD/chitosan/α-ZrP ternary biocomposite but also the recovered activity of the intercalated GOD improved by the co-intercalated chitosan. By depositing the GOD/chitosan/α-ZrP biocomposite film onto a glassy carbon electrode, the direct electrochemistry of the intercalated GOD was achieved with a fast electron transfer rate constant, k(s), of 7.48±3.52 s(-1). Moreover, this GOD/chitosan/α-ZrP biocomposite modified electrode exhibited a sensitive response to glucose in the linear range of 0.25-8.0 mM (R=0.9994, n=14), with a determination limit of 0.076 mM.
authors.affiliation: School of Chemistry and Chemical Engineering, Key Laboratory of Coordination Chemistry of Jiangxi Province, Institute of Applied Chemistry, Jinggangshan University, Ji'an, Jiangxi 343009, China. Electronic address: llm24@126.com.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Liu
authors.forename: Jiwu
authors.initials: J
authors.lastname: Wen
authors.forename: Lijun
authors.initials: L
authors.lastname: Liu
authors.forename: Deyong
authors.initials: D
authors.lastname: He
authors.forename: Ren-yun
authors.initials: RY
authors.lastname: Kuang
authors.forename: Taqing
authors.initials: T
authors.lastname: Shi
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D015374:Biosensing Techniques; D048271:Chitosan / Q000737:chemistry*; D055664:Electrochemical Techniques; D004566:Electrodes; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D015040:Zirconium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D004800:Enzymes, Immobilized; C027006:zirconium phosphate; D048271:Chitosan; D015040:Zirconium; D005949:Glucose Oxidase; D005947:Glucose
keywords: Biosensor; Co-intercalation; Direct electron transfer; Glucose oxidase; α-Zirconium phosphate