title: Identification of a new stromal cell type involved in the regulation of inflamed B cell follicles.
abstract: Lymph node (LN) stromal cells provide survival signals and adhesive substrata to lymphocytes. During an immune response, B cell follicles enlarge, questioning how LN stromal cells manage these cellular demands. Herein, we used a murine fate mapping system to describe a new stromal cell type that resides in the T cell zone of resting LNs. We demonstrated that upon inflammation, B cell follicles progressively trespassed into the adjacent T cell zone and surrounded and converted these stromal cells into CXCL13 secreting cells that in return delineated the new boundaries of the growing follicle. Acute B cell ablation in inflamed LNs abolished CXCL13 secretion in these cells, while LT-β deficiency in B cells drastically affected this conversion. Altogether, we reveal the existence of a dormant stromal cell subset that can be functionally awakened by B cells to delineate the transient boundaries of their expanding territories upon inflammation.
authors.affiliation: Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille University, UM2, Marseille, France ; Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, Marseille, France ; Centre National de la Recherche Scientifique (CNRS), UMR7280, Marseille, France.
authors.forename: Cyrille
authors.initials: C
authors.lastname: Mionnet
authors.forename: Isabelle
authors.initials: I
authors.lastname: Mondor
authors.forename: Audrey
authors.initials: A
authors.lastname: Jorquera
authors.forename: Marie
authors.initials: M
authors.lastname: Loosveld
authors.forename: Julien
authors.initials: J
authors.lastname: Maurizio
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Arcangeli
authors.forename: Nancy H
authors.initials: NH
authors.lastname: Ruddle
authors.forename: Jonathan
authors.initials: J
authors.lastname: Nowak
authors.forename: Michel
authors.initials: M
authors.lastname: Aurrand-Lions
authors.forename: Hervé
authors.initials: H
authors.lastname: Luche
authors.forename: Marc
authors.initials: M
authors.lastname: Bajénoff
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000473:pathology*; D054382:Chemokine CXCL13 / Q000378:metabolism; D003713:Dendritic Cells / Q000473:pathology; D005347:Fibroblasts / Q000473:pathology; D007249:Inflammation / Q000276:immunology* / Q000473:pathology*; D008198:Lymph Nodes / Q000473:pathology; D008212:Lymphocyte Depletion; D008214:Lymphocytes / Q000473:pathology; D051379:Mice; D054380:Receptors, CXCR5 / Q000172:deficiency / Q000378:metabolism; D017464:Receptors, Complement 3d / Q000378:metabolism; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D013601:T-Lymphocytes
chemical_list: D054382:Chemokine CXCL13; D054380:Receptors, CXCR5; D017464:Receptors, Complement 3d