title: One-step column chromatographic extraction with gradient elution followed by automatic separation of volatiles, flavonoids and polysaccharides from Citrus grandis.
abstract: Citrus grandis Tomentosa is widely used in traditional Chinese medicine and health foods. Its functional components include volatiles, flavonoids and polysaccharides which cannot be effectively extracted through traditional methods. A column chromatographic extraction with gradient elution was developed for one-step extraction of all bioactive substances from C. grandis. Dried material was loaded into a column with petroleum ether: ethanol (8:2, PE) and sequentially eluted with 2-fold PE, 3-fold ethanol: water (6:4) and 8-fold water. The elutes was separated into an ether fraction containing volatiles and an ethanol-water fraction containing flavonoids and polysaccharides. The later was separated into flavonoids and polysaccharides by 80% ethanol precipitation of polysaccharides. Through this procedure, volatiles, flavonoids and polysaccharides in C. grandis were simultaneously extracted at 98% extraction rates and simply separated at higher than 95% recovery rates. The method provides a simple and high-efficient extraction and separation of wide range bioactive substances.
authors.affiliation: College of Chemistry and Life Sciences, Guangdong University of Petrochemical Technology, 139 Guandu Road 2, Maoming City, Guangdong 525000, China; Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, China.
authors.forename: Han-Bing
authors.initials: HB
authors.lastname: Han
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Rui-Lin
authors.initials: RL
authors.lastname: Hao
authors.forename: Ya-Fei
authors.initials: YF
authors.lastname: Chen
authors.forename: He
authors.initials: H
authors.lastname: Ni
authors.forename: Hai-Hang
authors.initials: HH
authors.lastname: Li
mesh_terms: D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D002957:Citrus / Q000737:chemistry*; D053138:Cyclohexenes / Q000032:analysis; D005419:Flavonoids / Q000032:analysis*; D005504:Food Analysis; D000077222:Limonene; D010936:Plant Extracts / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis*; D013729:Terpenes / Q000032:analysis; D055549:Volatile Organic Compounds / Q000032:analysis*
chemical_list: D053138:Cyclohexenes; D005419:Flavonoids; D010936:Plant Extracts; D011134:Polysaccharides; D013729:Terpenes; D055549:Volatile Organic Compounds; D000077222:Limonene
keywords: Citrus grandis Tomentosa; Column chromatographic extraction; Flavonoids; Gradient elution; Health food; Polysaccharides; Traditional Chinese medicine; Volatiles