title: Expression of recombinant rotavirus proteins harboring antigenic epitopes of the hepatitis a virus polyprotein in insect cells.
abstract: Rotavirus and hepatitis A virus (HAV) spread by the fecal-oral route and infections are important in public health, especially in developing countries. Here, two antigenic epitopes of the HAV polyprotein, domain 2 (D2) and domain 3 (D3), were recombined with rotavirus VP7, generating D2/VP7 and D3/VP7, cloned in a baculovirus expression system, and expressed in Spodoptera frugiperda 9 (Sf9) insect cells. All were highly expressed, with peak expression 2 days post-infection. Western blotting and ELISA revealed that two chimeric proteins were antigenic, but only D2/VP7 was immunogenic and elicited neutralizing antibody responses against rotavirus and HAV by neutralization assay, implicating D2/VP7 as a multivalent subunit-vaccine Candidate for preventing both rotavirus and HAV infections.
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, Seoul 156-756.
authors.forename: Van Thai
authors.initials: VT
authors.lastname: Than
authors.forename: In Hyuk
authors.initials: IH
authors.lastname: Baek
authors.forename: Hee Young
authors.initials: HY
authors.lastname: Lee
authors.forename: Jong Bum
authors.initials: JB
authors.lastname: Kim
authors.forename: Dong Hwa
authors.initials: DH
authors.lastname: Shon
authors.forename: In Sik
authors.initials: IS
authors.lastname: Chung
authors.forename: Wonyong
authors.initials: W
authors.lastname: Kim
keywords: Hepatitis A virus; Recombinant chimera protein; Rotavirus