title: Effects of ovalbumin glycoconjugates on alleviation of orally induced egg allergy in mice via dendritic-cell maturation and T-cell activation.
abstract: METHODS
  Glycation of allergens via Maillard reaction or chemical conjugation has been shown to influence susceptibility to food-induced allergies. It is hypothesized that mucosal immune response bias can be favorably altered by orally administering various forms of glycated ovalbumin (OVA).
  
  
  RESULTS
  Groups of Balb/c mice (n = 10) were orally sensitized to OVA and administered various forms of glycated OVA (glucose, mannose, glucomannan, galactomannan, and a mixture containing OVA and glucomannan). Outcomes post oral challenge were measured as clinical allergic signs, serum histamine, mouse mast cell protease 1 (MMCP-1), antibody activity, type-1/2 cytokines, percentage of T-regulatory cells (T-regs) and in vitro dendritic cell, and T-cell-related mechanisms. Clinical signs and specific IgE were decreased (p ≤ 0.05), and T-reg cell percentage was increased in the mannose and glucomannan treated groups. The OVA-mannose group also had less histamine, MMCP-1, specific IgG, IL-4 and IL-17, and more IL-12p70 (p ≤ 0.05). Other parameters measured did not differ significantly among groups. Also, OVA-glycated mannose reduced maturation and uptake by dendritic cells. Less activation of T cells and type-2 cytokine response in DC-T-cell cocultures were observed with OVA-glycated mannose stimulation.
  
  
  CONCLUSIONS
  This study validates, for the first time, the use of OVA-glycated mannose and glucomannan for potential beneficial dietary interventions for allergy.
authors.affiliation: Department of Food Science, Ontario Agricultural College, University of Guelph, Guelph, Canada.
authors.forename: Prithy
authors.initials: P
authors.lastname: Rupa
authors.forename: Soichiro
authors.initials: S
authors.lastname: Nakamura
authors.forename: Shigeru
authors.initials: S
authors.lastname: Katayama
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Mine
mesh_terms: D000284:Administration, Oral; D000818:Animals; D053818:Chymases / Q000235:genetics / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology*; D004195:Disease Models, Animal; D021181:Egg Hypersensitivity / Q000276:immunology* / Q000517:prevention & control*; D005260:Female; D006001:Glycoconjugates / Q000008:administration & dosage*; D006632:Histamine / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation; D015416:Maillard Reaction; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D006001:Glycoconjugates; D006632:Histamine; D010047:Ovalbumin; D053818:Chymases; C509230:Mcpt1 protein, mouse
keywords: Food allergy; Glucomannan; Glycation; Immune modulation; d-mannose