title: Evaluation of lipoic acid topical application on rats skin wound healing.
abstract: OBJECTIVE
  To evaluate the effects of lipoic acid (thioctic acid) topical application on wound healing on rats skin, and the consequences of lipoic acid nanoencapsulation on this process.
  
  
  METHODS
  The model used was the healing activity on wounds induced by surgical incision on rats skin (n = 44). The parameters analyzed (11 days) were wound healing rate and histology (vascular proliferation, polymorphonuclear or mononuclear cells, and collagen synthesis or reepithelialization), after application of free lipoic acid or lipoic acid- loaded nanocapsules. The antioxidant activity of these formulations was evaluated by lipid peroxidation test.
  
  
  RESULTS
  It was demonstrated for the first time that the topical application of lipoic acid improves wound healing. On the seventh day after surgery, the animals treated with lipoic acid showed increased healing rate (60.7 ± 8.4%) compared to the negative control group (43.0 ± 17.4%), as so improvement of histological parameters. The nanoencapsulation reverted the pro-oxidant activity presented in vitro by lipoic acid, whereas diminished wound repair.
  
  
  CONCLUSIONS
  The topical application of lipoic acid produced an increase in the skin wound healing, which may be related to its pro-oxidant activity. On the other hand, the nanoencapsulation of the lipoic acid reversed the pro-oxidant activity, although presented minor healing activity.
authors.forename: Irene Clemes
authors.initials: IC
authors.lastname: Külkamp-Guerreiro
authors.forename: Marielly Nunes
authors.initials: MN
authors.lastname: Souza
authors.forename: Mariana Domingues
authors.initials: MD
authors.lastname: Bianchin
authors.forename: Mateus
authors.initials: M
authors.lastname: Isoppo
authors.forename: Joana Sachetti
authors.initials: JS
authors.lastname: Freitas
authors.forename: João Alex
authors.initials: JA
authors.lastname: Alves
authors.forename: Anna Paula
authors.initials: AP
authors.lastname: Piovezan
authors.forename: Adriana Raffin
authors.initials: AR
authors.lastname: Pohlmann
authors.forename: Sílvia Stanisçuaski
authors.initials: SS
authors.lastname: Guterres
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000378:metabolism; D004195:Disease Models, Animal; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D053769:Nanocapsules / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012867:Skin / Q000187:drug effects* / Q000473:pathology; D008063:Thioctic Acid / Q000008:administration & dosage* / Q000378:metabolism; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D053769:Nanocapsules; D017382:Reactive Oxygen Species; D008063:Thioctic Acid