title: Atmospheric pressure plasma polymers for tuned QCM detection of protein adhesion.
abstract: Our efforts have been concentrated in preparing plasma polymeric thin layers at atmospheric pressure grown on Quartz Crystal Microbalance-QCM electrodes for which the non-specific absorption of proteins can be efficiently modulated, tuned and used for QCM biosensing and quantification. Plasma polymerization reaction at atmospheric pressure has been used as a simple and viable method for the preparation of QCM bioactive surfaces, featuring variable protein binding properties. Polyethyleneglycol (ppEG), polystyrene (ppST) and poly(ethyleneglycol-styrene) (ppST-EG) thin-layers have been grown on QCM electrodes. These layers were characterized by Atomic Force Microscopy (AFM), Contact angle measurements, Fourier transform infrared (FTIR) and X-ray photoelectron spectroscopy (XPS). The plasma ppST QCM electrodes present a higher adsorption of Concanavalin A (ConA) and Bovine Serum Albumin (BSA) proteins when compared with the commercial coated polystyrene (ppST) ones. The minimum adsorption was found for ppEG, surface, known by their protein anti-fouling properties. The amount of adsorbed proteins can be tuned by the introduction of PEG precursors in the plasma discharge during the preparation of ppST polymers.
authors.affiliation: Faculty of Physics, Alexandru Ioan Cuza University of Iasi, 700506 Iasi, Romania; Institut Européen des Membranes, Adaptive Supramolecular Nanosystems Group, ENSCM-UMII-CNRS UMR-5635, Place Eugène Bataillon, CC 047, F-34095 Montpellier, France.
authors.forename: G B
authors.initials: GB
authors.lastname: Rusu
authors.forename: M
authors.initials: M
authors.lastname: Asandulesa
authors.forename: I
authors.initials: I
authors.lastname: Topala
authors.forename: V
authors.initials: V
authors.lastname: Pohoata
authors.forename: N
authors.initials: N
authors.lastname: Dumitrascu
authors.forename: M
authors.initials: M
authors.lastname: Barboiu
mesh_terms: D000042:Absorption; D001274:Atmospheric Pressure; D015374:Biosensing Techniques / Q000379:methods*; D018625:Microscopy, Atomic Force; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D058525:Quartz Crystal Microbalance Techniques; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013499:Surface Properties
chemical_list: D011108:Polymers; D011137:Polystyrenes; D011506:Proteins; D012710:Serum Albumin, Bovine
keywords: Plasma polymerization at atmospheric pressure; Protein absorption; Quartz crystal microbalance—QCM; Thin films