title: Relationship between expression of COX-2, TNF-α, IL-6 and autoimmune-type recurrent miscarriage.
abstract: OBJECTIVE
  To investigate the roles of COX-2, TNF-α, IL-6 in the pathogenesis of autoimmune-type recurrent spontaneous abortion (RSA).
  
  
  METHODS
  RT-PCR was used to detect the mRNA of COX-2, TNF-α, IL-6 in the trophoblast cells of murine RSA and normal pregnant models. The COX-2, TNF-α, IL-6 protein expressions were determined by using immunohistochemisry staining method. The COX-2, TNF-α, IL-6 protein expressions were determined by ELISA.
  
  
  RESULTS
  The embryo loss rates in experiment group was significantly higher than that in normal pregnancy control group, the expression of COX-2, TNF-α, IL-6 in the trophoblast cells of murine RSA and normal pregnant models. The expression of COX-2 in autoimmune-type recurrent spontaneous abortion was significantly lesser than in normal pregnant models. The expression of TNF-α, IL-6 in autoimmune-type recurrent spontaneous abortion was significantly higher than in normal pregnant models. There was a positively correlation between TNF-α and IL-6. There was no relationship between COX-2, TNF-α and IL-6.
  
  
  CONCLUSIONS
  The abnormal expression of COX-2, TNF-α and IL-6 may result in RSA.
authors.affiliation: Department of Gynaecology and Obstetrics, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu 223300, China.
authors.forename: Fu
authors.initials: F
authors.lastname: Hua
authors.forename: Chang-Hua
authors.initials: CH
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Hong-Ge
authors.initials: HG
authors.lastname: Xu
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D000818:Animals; D015551:Autoimmunity / Q000235:genetics* / Q000276:immunology*; D051546:Cyclooxygenase 2 / Q000032:analysis / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D004622:Embryo, Mammalian / Q000737:chemistry / Q000378:metabolism; D005260:Female; D015850:Interleukin-6 / Q000032:analysis / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D008297:Male; D033261:Maternal-Fetal Relations; D051379:Mice; D008808:Mice, Inbred CBA; D018709:Statistics, Nonparametric; D014327:Trophoblasts / Q000737:chemistry / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000097:blood / Q000235:genetics / Q000378:metabolism*
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; C577686:interleukin-6, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2
keywords: Animal model; Autoimmune; Maternal-fetal interface; Recurrent miscarriage