title: Evolution of B cell responses to Clec9A-targeted antigen.
abstract: The response of B cells to Ag targeted to Clec9A on dendritic cells was followed using the hapten nitrophenol (NP) conjugated to rat Ig carrier. Injection of small amounts of NP conjugated to anti-Clec9A in the absence of adjuvants gave high and very prolonged Ab responses, approaching those obtained by high doses of nontargeted NP-protein conjugates with alum adjuvant. The response to NP-anti-Clec9A included the transient formation of germinal centers, maturation of Ab affinity, and some memory B cell formation. Serum Ab titers remained high 35 wk postimmunization, well after the initial follicular response had faded. The results suggest Clec9A-targeting strategies for improving Ab responses to vaccine Ags.
authors.affiliation: The Walter and Eliza Hall Institute, Parkville, Victoria 3052, Australia;
authors.forename: Hae-Young
authors.initials: HY
authors.lastname: Park
authors.forename: Amanda
authors.initials: A
authors.lastname: Light
authors.forename: Mireille H
authors.initials: MH
authors.lastname: Lahoud
authors.forename: Irina
authors.initials: I
authors.lastname: Caminschi
authors.forename: David M
authors.initials: DM
authors.lastname: Tarlinton
authors.forename: Ken
authors.initials: K
authors.lastname: Shortman
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D007136:Immunoglobulins / Q000276:immunology; D007156:Immunologic Memory; D037181:Lectins, C-Type / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009596:Nitrophenols / Q000276:immunology; D011971:Receptors, Immunologic / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; C529914:Clec9a protein, mouse; D007136:Immunoglobulins; D037181:Lectins, C-Type; D009596:Nitrophenols; D011971:Receptors, Immunologic