title: Association of TNF-α -308 G > A and ACE I/D gene polymorphisms in hemodialysis patients with arteriovenous fistula thrombosis.
abstract: OBJECTIVE
  Tumor necrosis factor-α (TNF - α) -308 G > A promoter polymorphism seems to be associated with adverse clinical outcome in hemodialysis patients (HD). Angiotensin-converting enzyme (ACE) gene may be the causative factor contributing to the deterioration of renal functions. The aim of this study was to investigate the relationship between vascular access failure and the genetic polymorphisms of ACE and TNF-α gene.
  
  
  METHODS
  We enrolled and genotyped 47 HD patients with arteriovenous fistula (AVF) thrombosis, 51 HD patients without AVF thrombosis, and 40 healthy controls. The genotypes of these polymorphisms were determined by polymerase chain reaction and restriction fragment length polymorphism.
  
  
  RESULTS
  The genotype distribution of TNF-α -308 G > A in patients with thrombosis was significantly different from the patients without thrombosis (p = 0.008). There was no significant difference between the two groups in terms of ACE I/D polymorphism (p = 0.213).
  
  
  CONCLUSIONS
  Our results propose that TNF-α -308 G > A genotype may be a potential genetic marker on HD patients with AVF thrombosis.
authors.affiliation: Department of Medical Biology, Erciyes University Medical School, 38039, Kayseri, Turkey, efefunda@yahoo.com.
authors.forename: Elif Funda
authors.initials: EF
authors.lastname: Sener
authors.forename: Serpil
authors.initials: S
authors.lastname: Taheri
authors.forename: Keziban
authors.initials: K
authors.lastname: Korkmaz
authors.forename: Gokmen
authors.initials: G
authors.lastname: Zararsiz
authors.forename: Faruk
authors.initials: F
authors.lastname: Serhatlioglu
authors.forename: Aydin
authors.initials: A
authors.lastname: Unal
authors.forename: Omer Naci
authors.initials: ON
authors.lastname: Emirogullari
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Ozkul
mesh_terms: D000328:Adult; D000368:Aged; D001166:Arteriovenous Shunt, Surgical / Q000009:adverse effects*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics; D006435:Renal Dialysis; D013927:Thrombosis / Q000209:etiology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D014654:Vascular Patency
chemical_list: D014409:Tumor Necrosis Factor-alpha; D007703:Peptidyl-Dipeptidase A