title: [Effects of plumbagin on expression of TNF-alpha and PDGF-BB in human hepatic stellate cells activated by leptin].
abstract: OBJECTIVE
  To investigate the effect of plumbagin on the expression of TNF-alpha and PDGF-BB in human hepatic stellate cells (HSC-LX2) activated by Leptin.
  
  
  METHODS
  HSC-LX2 were cultured in vitro and stimulated by Leptin for 24 hours then treated with different concentrations of plumbagin for 24 hours, the expressions of TNF-alpha mRNA and PDGF-BB mRNA were determined by Realtime quantitative PCR, the protein expressions of TNF-alpha and PDGF-BB were determined-by Western blotting.
  
  
  RESULTS
  The expressions of TNF-alpha mRNA and PDGF-BB mRNA of treatment groups were significantly reduced, especially in high dose group (P < 0.01), and Western blotting analyses revealed similar trends in protein expression.
  
  
  CONCLUSIONS
  Plumbagin may prevent the formation of hepatic fibrosis and its mechanism may be related to decreasing the level of mRNA of TNF-alpha and PDGF-BB and the protein of PDGF-BB.
authors.affiliation: Physiological Department of Guangxi Traditional Chinese Medical University, Nanning 530001, China.
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Liu
authors.forename: Tie-Jian
authors.initials: TJ
authors.lastname: Zhao
authors.forename: Yue
authors.initials: Y
authors.lastname: Peng
authors.forename: Xue-Lin
authors.initials: XL
authors.lastname: Duan
authors.forename: Yan-Fei
authors.initials: YF
authors.lastname: Wei
mesh_terms: D000077214:Becaplermin; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D055166:Hepatic Stellate Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D020738:Leptin / Q000494:pharmacology; D008103:Liver Cirrhosis / Q000378:metabolism / Q000473:pathology; D009285:Naphthoquinones / Q000494:pharmacology*; D029606:Plumbaginaceae / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D020574:Proto-Oncogene Proteins c-sis / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D020738:Leptin; D009285:Naphthoquinones; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D000077214:Becaplermin; C014758:plumbagin