title: Glucose regulates protein kinase CK2 in pancreatic β-cells and its interaction with PDX-1.
abstract: The pancreatic duodenal homeodomain transcription factor PDX-1 plays a pivotal role in the development of the pancreas and the maintenance of glucose homeostasis by pancreatic β-cells. Recently, we found that the highly conserved, ubiquitously expressed tetrameric Ser/Thr protein kinase CK2, which is formed by two catalytic subunits (α and/or α') and two non-catalytic subunits (β), phosphorylates PDX-1. So far, only little is known about CK2 in pancreatic β-cells and how this enzyme is regulated in these cells. In the present study, we found that (i) CK2 binds to PDX-1, (ii) the binding between CK2 and PDX-1 is regulated by glucose, (iii) glucose modulates the subcellular localization of PDX-1 and CK2 and (iv) the kinase activity is also regulated by glucose. Our novel data indicate that CK2 is a co-factor of PDX-1 in response to glucose in pancreatic β-cells.
authors.affiliation: Medical Biochemistry and Molecular Biology, Saarland University, Building 44, D-66424 Homburg, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Welker
authors.forename: Claudia
authors.initials: C
authors.lastname: Götz
authors.forename: Christina
authors.initials: C
authors.lastname: Servas
authors.forename: Matthias W
authors.initials: MW
authors.lastname: Laschke
authors.forename: Michael D
authors.initials: MD
authors.lastname: Menger
authors.forename: Mathias
authors.initials: M
authors.lastname: Montenarh
mesh_terms: D000818:Animals; D047390:Casein Kinase II / Q000378:metabolism*; D002460:Cell Line; D005947:Glucose / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010179:Pancreas / Q000378:metabolism / Q000473:pathology*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
chemical_list: D018398:Homeodomain Proteins; D015534:Trans-Activators; C084010:pancreatic and duodenal homeobox 1 protein; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases; D005947:Glucose
keywords: Glucose; Pancreatic islets; Protein kinase CK2; Protein protein interaction; Subcellular localization