title: Concentration-dependent viscosity of binary and ternary mixtures of nonassociating proteins: measurement and analysis.
abstract: Using a recently developed automated viscometer (Grupi, A.; Minton, A. P. Anal. Chem. 2012, 84, 10732-10736), the dependence of solution viscosity upon the concentrations of bovine serum albumin, hen egg ovomucoid, and human fibrinogen have been measured individually and in binary and ternary mixtures over a wide range of compositions and at total concentrations of up to 300 g/L. The concentration dependence of viscosity of all solutions is quantitatively described over the entire range of concentrations and compositions by a semiempirical equation requiring specification of only two composition-independent global parameters per protein.
authors.affiliation: Section on Physical Biochemistry, Laboratory of Biochemistry and Genetics, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, U.S. Department of Health and Human Services , Building 8, Room 226, Bethesda, Maryland 20892-0830, United States.
authors.forename: Asaf
authors.initials: A
authors.lastname: Grupi
authors.forename: Allen P
authors.initials: AP
authors.lastname: Minton
mesh_terms: D000818:Animals; D001120:Arginine / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D002645:Chickens; D005340:Fibrinogen / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D010059:Ovomucin / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D012996:Solutions / Q000737:chemistry; D014783:Viscosity
chemical_list: D012996:Solutions; D012710:Serum Albumin, Bovine; D010059:Ovomucin; D005340:Fibrinogen; D001120:Arginine