title: Riboswitches in eubacteria sense the second messenger c-di-AMP.
abstract: Cyclic di-adenosine monophosphate (c-di-AMP) is a recently discovered bacterial second messenger implicated in the control of cell wall metabolism, osmotic stress responses and sporulation. However, the mechanisms by which c-di-AMP triggers these physiological responses have remained largely unknown. Notably, a candidate riboswitch class called ydaO associates with numerous genes involved in these same processes. Although a representative ydaO motif RNA recently was reported to weakly bind ATP, we report that numerous members of this noncoding RNA class selectively respond to c-di-AMP with subnanomolar affinity. Our findings resolve the mystery regarding the primary ligand for this extremely common riboswitch class and expose a major portion of the super-regulon of genes that are controlled by the widespread bacterial second messenger c-di-AMP.
authors.affiliation: Department of Chemistry, Yale University, New Haven, Connecticut, USA.
authors.forename: James W
authors.initials: JW
authors.lastname: Nelson
authors.forename: Narasimhan
authors.initials: N
authors.lastname: Sudarsan
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Furukawa
authors.forename: Zasha
authors.initials: Z
authors.lastname: Weinberg
authors.forename: Joy X
authors.initials: JX
authors.lastname: Wang
authors.forename: Ronald R
authors.initials: RR
authors.lastname: Breaker
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D015226:Dinucleoside Phosphates / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D009690:Nucleic Acid Conformation; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism; D058928:Riboswitch / Q000502:physiology*; D015398:Signal Transduction; D015003:Yeasts
chemical_list: D015226:Dinucleoside Phosphates; D022661:RNA, Untranslated; D058928:Riboswitch; C528998:cyclic diadenosine phosphate