title: [Comparative study on the protein maps of different parts of Cervus nippon antler by two-dimensional electrophoresis].
abstract: OBJECTIVE
  To optimize the two-dimensional electrophoresis (2-DE) method for the proteome analysis of the Cervus nippon antler, and compare the protein maps of different parts of Cervus nippon antler.
  
  
  METHODS
  The total proteins of Cervus nippon antler were extracted by protein lysate containing 7 mol/L Urea, 4% CHAPS, 2 mol/L Thiourea, 65 mmol/L DTT, 1 mmol/L PMSF and 0.2% Bio-Lyte. The proteins were separated by immobilized pH 3 - 10 linear gradient (IPG), 7 cm strips as the first dimension. Isoelectric focusing conditions were optimized. 12% SDS-PAGE was used as the second dimension electrophoresis. The gels were stained with Coomassie brilliant blue and analyzed by PDQuest analysis software.
  
  
  RESULTS
  The contents of total protein and the numbers of protein points of three different parts of Cervus nippon antler reduced gradually from the top to the bottom. Comparing three maps of different parts of Cervus nippon antler, there were 18 different protein points. Isoelectric point, molecular weight and gray value of each different protein point were calculated.
  
  
  CONCLUSIONS
  An optimized two-dimensional electrophoresis method for the proteome analysis of the Cervus nippon antler is established. The 2-DE profiles of different parts of Cervus nippon antler exist obvious differences. The different protein points can be used as reference for Cervus nippon antler quality control and evaluation.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beijing 100102, China. 370913575@qq.com
authors.forename: Qian-Nan
authors.initials: QN
authors.lastname: Ding
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Wang
authors.forename: Jun-Dong
authors.initials: JD
authors.lastname: Dai
authors.forename: Ling
authors.initials: L
authors.lastname: Dong
mesh_terms: D000818:Animals; D000999:Antlers / Q000737:chemistry*; D003670:Deer; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008297:Male; D008419:Materia Medica / Q000737:chemistry; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D020543:Proteome / Q000032:analysis*; D011786:Quality Control; D012984:Software
chemical_list: D008419:Materia Medica; D011506:Proteins; D020543:Proteome