title: Analysis of differentially expressed genes in placental tissues of preeclampsia patients using microarray combined with the Connectivity Map database.
abstract: Preeclampsia (PE), which affects 2-7% of human pregnancies, causes significant maternal and neonatal morbidity and mortality. To better understand the pathophysiology of PE, the gene expression profiles of placental tissue from 5 controls and 5 PE patients were assessed using microarray. A total of 224 transcripts were significantly differentially expressed (>2-fold change and q value <0.05, SAM software). Gene Ontology (GO) enrichment analysis indicated that genes involved in hypoxia and oxidative and reductive processes were significantly changed. Three differentially expressed genes (DEGs) involved in these biological processes were further verified by quantitative real-time PCR. Finally, the potential therapeutic agents for PE were explored via the Connectivity Map database. In conclusion, the data obtained in this study might provide clues to better understand the pathophysiology of PE and to identify potential therapeutic agents for PE patients.
authors.affiliation: Department of Obstetrics & Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Peking Union Medical College, Beijing, PR China.
authors.forename: Y
authors.initials: Y
authors.lastname: Song
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: S
authors.initials: S
authors.lastname: Huang
authors.forename: L
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000328:Adult; D044466:Asian People; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D015687:Cell Hypoxia; D002585:Cesarean Section; D002681:China; D019295:Computational Biology; D030562:Databases, Protein; D005103:Expert Systems; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D063990:Gene Ontology; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010084:Oxidation-Reduction; D011225:Pre-Eclampsia / Q000378:metabolism* / Q000503:physiopathology; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics / Q000378:metabolism*; D047928:Premature Birth; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers; D011257:Pregnancy Proteins
keywords: Connectivity Map database; Expression profiles; Microarray; Preeclampsia