title: Injectable hydrogel incorporating with nanoyarn for bone regeneration.
abstract: Traditional bone grafting requires an open surgical approach to the graft application sites with the attendant complications of a large surgical scar, increased pain and a longer post-operative recovery. To overcome these limitations, there is a great need for the development of better bone graft substitutes. In this study, we developed a novel injectable system which was a biomimetic bone substitute consisted of Poly (L-lactide-co-ε-caprolactone) (P(LLA-CL)) nanoyarns suspended in type I collagen hydrogel (Col). A dynamic liquid support system was employed to fabricate continuous P(LLA-CL) nanoyarns. The electrospun long nanoyarns were chopped into short nanoyarns before they were incorporated into Col. The result of rheological evaluation showed that the mechanical property of Col was enhanced after the nanoyarns were incorporated into it. The mixture of Col and nanoyarn could be smoothly injected out of 16 gauge needle. In vitro study showed that human mesenchymal stem cells (hMSCs) proliferated well on Col with nanoyarns. Alkaline phosphatase activity and osteocalcin expression of hMSCs on hydrogel with nanoyarns were much higher than those on control groups. This study highlights the potential of using a novel injectable biomimetic scaffold for bone regeneration.
authors.affiliation: a State Key Lab for Modification of Chemical Fiber & Polymer Materials , College of Material Science and Engineering, Donghua University , Shanghai 201620 , P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Jianchao
authors.initials: J
authors.lastname: Zhan
authors.forename: Yan
authors.initials: Y
authors.lastname: Su
authors.forename: Tong
authors.initials: T
authors.lastname: Wu
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
authors.forename: Susan
authors.initials: S
authors.lastname: Liao
authors.forename: Xiumei
authors.initials: X
authors.lastname: Mo
mesh_terms: D040761:Biomimetic Materials / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D024042:Collagen Type I / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D007267:Injections; D055595:Mechanical Phenomena; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D024042:Collagen Type I; D020100:Hydrogels; D011091:Polyesters; C062968:lactide-caprolactone copolymer