title: JNK is necessary for oligodendrocyte precursor cell proliferation induced by the conditioned medium from B104 neuroblastoma cells.
abstract: The conditioned medium from B104 neuroblastoma cells (B104CM) induces proliferation of oligodendrocyte progenitor cells (OPCs) in vitro. Our previous study showed that phosphorylation of extracellular signal-regulated protein kinase(s), not PI3K or p38, is key to B104CM-induced OPC proliferation. However, whether there are still other signaling pathways that are also involved in B104CM-induced proliferation remains unknown. In the present study, we evaluated the implication of c-Jun N-terminal kinase (JNK) signaling pathway in the B104CM-induced proliferation of OPCs using the specific inhibitor of JNK. We provided convincing evidence for the first time that the phosphorylation of JNK is necessary for OPC proliferation induced by B104CM in vitro. Moreover, the activation of JNK results in subsequent expressions of c-jun, c-fos, and c-myc, which initiates proliferation of OPCs. Collectively, these results suggest that JNK is also necessary for B104CM-induced OPC proliferation.
authors.forename: Jing-Xing
authors.initials: JX
authors.lastname: Zhang
authors.forename: Yi-Fan
authors.initials: YF
authors.lastname: Feng
authors.forename: Qi
authors.initials: Q
authors.lastname: Qi
authors.forename: Lin
authors.initials: L
authors.lastname: Shen
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Jian-Sheng
authors.initials: JS
authors.lastname: Zhou
authors.forename: He-Zuo
authors.initials: HZ
authors.lastname: Lü
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Hu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D048670:MAP Kinase Kinase 4 / Q000378:metabolism*; D058953:Neural Stem Cells / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D009447:Neuroblastoma / Q000378:metabolism*; D009836:Oligodendroglia / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D017077:Culture Media, Conditioned; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D016271:Proto-Oncogene Proteins c-myc; D048670:MAP Kinase Kinase 4