title: Recombinant DNA production of spider silk proteins.
abstract: Spider dragline silk is considered to be the toughest biopolymer on Earth due to an extraordinary combination of strength and elasticity. Moreover, silks are biocompatible and biodegradable protein-based materials. Recent advances in genetic engineering make it possible to produce recombinant silks in heterologous hosts, opening up opportunities for large-scale production of recombinant silks for various biomedical and material science applications. We review the current strategies to produce recombinant spider silks.
authors.affiliation: Department of Biomedical Engineering, Tufts University, Medford, MA, 02155, USA.
authors.forename: Olena
authors.initials: O
authors.lastname: Tokareva
authors.forename: Valquíria A
authors.initials: VA
authors.lastname: Michalczechen-Lacerda
authors.forename: Elíbio L
authors.initials: EL
authors.lastname: Rech
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics / Q000378:metabolism*; D001704:Biopolymers / Q000096:biosynthesis / Q000235:genetics; D001709:Biotechnology; D004274:DNA, Recombinant / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D047011:Silk / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D060829:Arthropod Proteins; D001704:Biopolymers; D004274:DNA, Recombinant; D011994:Recombinant Proteins; D047011:Silk