title: A peptide antagonist of ErbB receptors, Inherbin3, induces neurite outgrowth from rat cerebellar granule neurons through ErbB1 inhibition.
abstract: ErbB receptors not only function in cancer, but are also key developmental regulators in the nervous system. We previously identified an ErbB1 peptide antagonist, Inherbin3, that is capable of inhibiting tumor growth in vitro and in vivo. In this study, we found that inhibition of ErbB1 kinase activity and activation of ErbB4 by NRG-1β induced neurite extension, suggesting that ErbB1 and ErbB4 act as negative and positive regulators, respectively, of the neuritogenic response. Inherbin3, inhibited activation not only of ErbB1 but also of ErbB4 in primary neurons, strongly induced neurite outgrowth in rat cerebellar granule neurons, indicating that this effect mainly was due to inhibition of ErbB1 activation.
authors.affiliation: Laboratory of Neural Plasticity, Department of Neuroscience and Pharmacology, Faculty of Health Sciences, University of Copenhagen, Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark.
authors.forename: Ruodan
authors.initials: R
authors.lastname: Xu
authors.forename: Stanislava
authors.initials: S
authors.lastname: Pankratova
authors.forename: Søren Hofman
authors.initials: SH
authors.lastname: Christiansen
authors.forename: David
authors.initials: D
authors.lastname: Woldbye
authors.forename: Anne
authors.initials: A
authors.lastname: Højland
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Bock
authors.forename: Vladimir
authors.initials: V
authors.lastname: Berezin
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects*; D003594:Cytoplasmic Granules / Q000187:drug effects*; D017931:DNA Primers; D066246:ErbB Receptors / Q000037:antagonists & inhibitors* / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D016501:Neurites / Q000187:drug effects*; D010455:Peptides / Q000494:pharmacology*; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C000589693:inherbin3; D066246:ErbB Receptors