title: Ultrathin-yttrium phosphate-shelled polyacrylate-ferriferrous oxide magnetic microspheres for rapid and selective enrichment of phosphopeptides.
abstract: Rapid and selective enrichment of phosphopeptides from complex biological samples is essential and challenging in phosphorylated proteomics. We present the direct growth of the ultrathin YPO4 shell on the surface of polyacrylate capped secondary Fe3O4 microspheres (PA-Fe3O4@YPO4) for the rapid and selective trapping phosphopeptides from complex samples. The prepared PA-Fe3O4@YPO4 could be rapidly harvested in the presence of an applied magnetic field and easily re-dispersed in solutions after removing the external magnet. The ultrathin YPO4 shell on super-hydrophilic PA-Fe3O4 has the advantages of fast adsorption/desorption dynamics and low non-specific adsorption, thus trapping of phosphopeptides from the tryptic digests mixture of β-casein/BSA with molar ratio of 1/300 is achieved in 20s adsorption/desorption time. Two phosphopeptides can still be detected with a signal to noise ratio (S/N) over 3 when the amount of β-casein was as low as 8 fmol.
authors.affiliation: Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Sun
authors.forename: He-Fang
authors.initials: HF
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D000818:Animals; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008892:Milk / Q000737:chemistry; D010748:Phosphopeptides / Q000032:analysis / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry*; D059629:Signal-To-Noise Ratio; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015019:Yttrium / Q000737:chemistry*
chemical_list: D058185:Magnetite Nanoparticles; D010748:Phosphopeptides; D011108:Polymers; D015019:Yttrium
keywords: MALDI-TOF MS; Magnetic microsphere; Phosphopeptide; Ultrathin shell; Yttrium phosphate