title: Involvement of antioxidant defense system in solvent tolerance of Pseudomonas putida BCNU 106.
abstract: The highly solvent-tolerant bacterium Pseudomonas sp. BCNU 106 was investigated to elucidate the solvent tolerance under specific culture conditions with the presence of solvents and its adaptive mechanisms to those conditions with reference to the antioxidant system. When exposed to 10% toluene, Pseudomonas sp. BCNU 106 increased the generation of reactive oxygen species assessed by monitoring the oxidation of 2',7'-dichlorofluorescein. Typical antioxidant enzymes (viz. catalase, superoxide dismutase, and glutathione reductase) showed increased activity with prolonged incubation in 10% toluene. In addition, the levels of these antioxidant proteins were higher during exposure to 10% toluene than in toluene-free condition. The present study indicates that antioxidant defense activity is one of the adaptive and protective mechanisms developed to avoid the deleterious damage of organic solvents, especially toluene.
authors.affiliation: Interdisplinary Program for Biotechnology and Department of Biology, Changwon National University, Changwon, Korea.
authors.forename: Hye Jung
authors.initials: HJ
authors.lastname: Choi
authors.forename: Ju-Soon
authors.initials: JS
authors.lastname: Yoo
authors.forename: Yong Kee
authors.initials: YK
authors.lastname: Jeong
authors.forename: Woo Hong
authors.initials: WH
authors.lastname: Joo
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D004361:Drug Tolerance; D005980:Glutathione Reductase / Q000378:metabolism; D018384:Oxidative Stress; D016958:Pseudomonas putida / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D017382:Reactive Oxygen Species; D012997:Solvents / Q000378:metabolism* / Q000633:toxicity*; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism; D014050:Toluene / Q000378:metabolism / Q000633:toxicity
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D012997:Solvents; D014050:Toluene; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase
keywords: Antioxidant defense activity; Pseudomonas sp.; Solvent-tolerant bacterium