title: [Study on quality standard for mori cortex].
abstract: OBJECTIVE
  To establish a new evaluation model and compare the differences of Mori Cortex from different habitats at different harvest time.
  
  
  METHODS
  TLC method was used to identify sanggenon D in the sample with silica gel G plate and a mixture of chloroform-methanol-formic acid (5: 1:0. 3) as a developing solvent. UV was used for determination the centent of total flavonoids of Mori Cortex with sanggenon C as the reference substance. HPLC was applied for determination the contents of sanggenons C, D and DNJ of Mori Cortex.
  
  
  RESULTS
  In the TLC chromatogram, sanggenon D showed a distinct fluorescence spot under UV 365 nm with good separation. In UV, sanggenon C calibration curve showed a good linear relationship at the range of 146.8 - 734.0 microg/mL, the average recovery was 97.4% (RSD = 2.1%); For the HPLC quantitation method, sanggenons C, D and DNJ showed good linear within the scope of 700 - 4 000 microg, 500 - 3 000 microg and 4.8 - 96 microg, respectively. The average recovery of sanggenons C, D and DNJ were 98.8% (RSD = 2.6%), 99.1% (RSD = 2.2%) and 98.9% (RSD = 1.7%), respectively. The contents of index components in samples from different habitats at different harvest time were different.
  
  
  CONCLUSIONS
  The established method is reliable and accurate. It can be used for quality control of Mori Cortex.
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of TCM, Guangzhou 510006, China. dzt8716@163.com
authors.forename: Zhi-Tao
authors.initials: ZT
authors.lastname: Duan
authors.forename: Ying
authors.initials: Y
authors.lastname: Gao
authors.forename: Gang
authors.initials: G
authors.lastname: Zhou
mesh_terms: D017485:1-Deoxynojirimycin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000592:standards; D005419:Flavonoids / Q000032:analysis*; D007659:Ketones / Q000032:analysis; D031625:Morus / Q000737:chemistry*; D010598:Pharmacognosy / Q000592:standards; D024301:Plant Bark / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D007659:Ketones; D017485:1-Deoxynojirimycin