title: Development of azo-based fluorescent probes to detect different levels of hypoxia.
abstract: Let it shine: New hypoxia-sensitive fluorescent probes were developed; they consist of a rhodamine moiety with an azo group directly conjugated to the fluorophore. Because of an ultrafast conformational change around the NN bond, the compounds are nonfluorescent under normoxia. However, under hypoxia, the azo group is reduced, and a strongly fluorescent rhodamine derivative is released.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan).
authors.forename: Wen
authors.initials: W
authors.lastname: Piao
authors.forename: Satoru
authors.initials: S
authors.lastname: Tsuda
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Satoshi
authors.initials: S
authors.lastname: Maeda
authors.forename: Fengyi
authors.initials: F
authors.lastname: Liu
authors.forename: Shodai
authors.initials: S
authors.lastname: Takahashi
authors.forename: Yu
authors.initials: Y
authors.lastname: Kushida
authors.forename: Toru
authors.initials: T
authors.lastname: Komatsu
authors.forename: Tasuku
authors.initials: T
authors.lastname: Ueno
authors.forename: Takuya
authors.initials: T
authors.lastname: Terai
authors.forename: Toru
authors.initials: T
authors.lastname: Nakazawa
authors.forename: Masanobu
authors.initials: M
authors.lastname: Uchiyama
authors.forename: Keiji
authors.initials: K
authors.lastname: Morokuma
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nagano
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Hanaoka
mesh_terms: D000818:Animals; D001391:Azo Compounds / Q000737:chemistry*; D015687:Cell Hypoxia / Q000502:physiology*; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry / Q000378:metabolism; D008862:Microsomes, Liver / Q000737:chemistry / Q000378:metabolism; D011789:Quantum Theory; D051381:Rats
chemical_list: D001391:Azo Compounds; D005456:Fluorescent Dyes
keywords: azo compounds; bioorganic chemistry; fluorescent probes; imaging agents; oxygen concentration