title: Kinetics of myosin light chain kinase activation of smooth muscle myosin in an in vitro model system.
abstract: During activation of smooth muscle contraction, one myosin light chain kinase (MLCK) molecule rapidly phosphorylates many smooth muscle myosin (SMM) molecules, suggesting that muscle activation rates are influenced by the kinetics of MLCK-SMM interactions. To determine the rate-limiting step underlying activation of SMM by MLCK, we measured the kinetics of calcium-calmodulin (Ca²⁺CaM)-MLCK-mediated SMM phosphorylation and the corresponding initiation of SMM-based F-actin motility in an in vitro system with SMM attached to a coverslip surface. Fitting the time course of SMM phosphorylation to a kinetic model gave an initial phosphorylation rate, kp(o), of ~1.17 heads s⁻¹ MLCK⁻¹. Also, we measured the dwell time of single streptavidin-coated quantum dot-labeled MLCK molecules interacting with surface-attached SMM and phosphorylated SMM using total internal reflection fluorescence microscopy. From these data, the dissociation rate constant from phosphorylated SMM was 0.80 s⁻¹, which was similar to the kp(o) mentioned above and with rates measured in solution. This dissociation rate was essentially independent of the phosphorylation state of SMM. From calculations using our measured dissociation rates and Kd values, and estimates of SMM and MLCK concentrations in muscle, we predict that the dissociation of MLCK from phosphorylated SMM is rate-limiting and that the rate of the phosphorylation step is faster than this dissociation rate. Also, association with SMM (11-46 s⁻¹) would be much faster than with pSMM (<0.1-0.2 s⁻¹). This suggests that the probability of MLCK interacting with unphosphorylated versus phosphorylated SMM is 55-460 times greater. This would avoid sequestering MLCK to unproductive interactions with previously phosphorylated SMM, potentially leading to faster rates of phosphorylation in muscle.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Nevada School of Medicine , Reno, Nevada 99557, United States.
authors.forename: Feng
authors.initials: F
authors.lastname: Hong
authors.forename: Kevin C
authors.initials: KC
authors.lastname: Facemyer
authors.forename: Michael S
authors.initials: MS
authors.lastname: Carter
authors.forename: Del R
authors.initials: del R
authors.lastname: Jackson
authors.forename: Brian D
authors.initials: BD
authors.lastname: Haldeman
authors.forename: Nick
authors.initials: N
authors.lastname: Ruana
authors.forename: Cindy
authors.initials: C
authors.lastname: Sutherland
authors.forename: Michael P
authors.initials: MP
authors.lastname: Walsh
authors.forename: Christine R
authors.initials: CR
authors.lastname: Cremo
authors.forename: Josh E
authors.initials: JE
authors.lastname: Baker
mesh_terms: D008841:Actin Cytoskeleton / Q000737:chemistry / Q000378:metabolism; D000199:Actins / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D020013:Calcium Signaling; D002147:Calmodulin / Q000378:metabolism; D002645:Chickens; D004789:Enzyme Activation; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D007734:Kymography; D008747:Methylcellulose / Q000737:chemistry / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009219:Myosin-Light-Chain Kinase / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D024745:Smooth Muscle Myosins / Q000737:chemistry / Q000378:metabolism*; D013499:Surface Properties
chemical_list: D000199:Actins; D002147:Calmodulin; D055767:Immobilized Proteins; D008747:Methylcellulose; D009219:Myosin-Light-Chain Kinase; D024745:Smooth Muscle Myosins