title: Optical trapping in plant cells.
abstract: Optical tweezers allow noninvasive manipulation of subcellular compartments to study their physical interactions and attachments. By measuring (delay of) displacements, (semi-)quantitative force measurements within a living cell can be performed. In this chapter, we provide practical tips for setting up such experiments paying special attention to the technical considerations for integrating optical tweezers into a confocal microscope. Next, we describe some working protocols to trap intracellular structures in plant cells.
authors.affiliation: Laboratory of Cell Biology, Wageningen University, Wageningen, The Netherlands.
authors.forename: Tijs
authors.initials: T
authors.lastname: Ketelaar
authors.forename: Norbert
authors.initials: N
authors.lastname: de Ruijter
authors.forename: Stefan
authors.initials: S
authors.lastname: Niehren
mesh_terms: D007834:Lasers; D008853:Microscopy; D052898:Optical Tweezers; D059828:Plant Cells; D010944:Plants / Q000033:anatomy & histology / Q000648:ultrastructure