title: Natural regulatory T cells in isolated early responders compared with dual responders with allergic asthma.
abstract: BACKGROUND
  Natural regulatory T (Treg) cells are implicated in the regulation of the inflammatory response in patients with allergic asthma.
  
  
  OBJECTIVE
  We sought to determine changes in Treg cell numbers in the airways and peripheral blood of isolated early responder (IER) versus dual responder (DR) subjects with mild allergic asthma before and after allergen challenge.
  
  
  METHODS
  Induced sputum was collected from 22 subjects with allergic asthma (10 IERs and 12 DRs) and peripheral blood collected from 8 DRs with allergic asthma at 0, 7, and 24 hours after allergen challenge. Treg cells were identified by using fluorescently labeled antibodies to CD4 and forkhead box protein 3 and enumerated by using flow cytometry.
  
  
  RESULTS
  There was a significant increase in the percentage of sputum CD4(+) cells 24 hours after allergen challenge in both IERs and DRs. The percentage of sputum Treg cells significantly decreased 24 hours after challenge in DRs but not IERs. This change was significantly correlated with the magnitude of the late asthmatic response. There was also a significant increase in the absolute number of sputum CD4(+) cells and Treg cells at 24 hours in DRs only. The ratio of the number of Treg cells to CD4(+) cells at 24 hours was significantly smaller in DRs compared with that in IERs. None of the above changes were observed in peripheral blood.
  
  
  CONCLUSIONS
  DRs exhibit a diminished percentage of airway Treg cells after allergen challenge that is not observed in IERs and a significantly lower ratio of Treg cells to CD4(+) cells, which might contribute to the development of the late asthmatic response.
authors.affiliation: Firestone Institute of Respiratory Health, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada; Department of Medicine, Division of Respirology, Neurology and Rheumatology, Kurume University, Fukuoka, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kinoshita
authors.affiliation: Firestone Institute of Respiratory Health, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Adrian
authors.initials: A
authors.lastname: Baatjes
authors.affiliation: Firestone Institute of Respiratory Health, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Steven G
authors.initials: SG
authors.lastname: Smith
authors.affiliation: Firestone Institute of Respiratory Health, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Benny
authors.initials: B
authors.lastname: Dua
authors.affiliation: Firestone Institute of Respiratory Health, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Richard
authors.initials: R
authors.lastname: Watson
authors.affiliation: Department of Medicine, Division of Respirology, Neurology and Rheumatology, Kurume University, Fukuoka, Japan.
authors.forename: Tomotaka
authors.initials: T
authors.lastname: Kawayama
authors.affiliation: Firestone Institute of Respiratory Health, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Mark
authors.initials: M
authors.lastname: Larche
authors.affiliation: Firestone Institute of Respiratory Health, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Gail M
authors.initials: GM
authors.lastname: Gauvreau
authors.affiliation: Firestone Institute of Respiratory Health, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada. Electronic address: obyrnep@mcmaster.ca.
authors.forename: Paul M
authors.initials: PM
authors.lastname: O'Byrne
mesh_terms: D000293:Adolescent; D000328:Adult; D001249:Asthma / Q000276:immunology* / Q000503:physiopathology; D015704:CD4 Antigens / Q000032:analysis; D005260:Female; D005541:Forced Expiratory Volume; D051858:Forkhead Transcription Factors / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D013183:Sputum / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D015704:CD4 Antigens; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors
keywords: Allergic asthma; CD4(+) T cells; induced sputum; late asthmatic responses; regulatory T cells