title: Effect of hyperbaric oxygen therapy combined with autologous platelet concentrate applied in rabbit fibula fraction healing.
abstract: OBJECTIVE
  The purpose is to study the effects of hyperbaric oxygen therapy and autologous platelet concentrates in healing the fibula bone of rabbits after induced fractures.
  
  
  METHODS
  A total of 128 male New Zealand albino rabbits, between 6-8 months old, were subjected to a total osteotomy of the proximal portion of the right fibula. After surgery, the animals were divided into four groups (n = 32 each): control group, in which animals were subjected to osteotomy; autologous platelet concentrate group, in which animals were subjected to osteotomy and autologous platelet concentrate applied at the fracture site; hyperbaric oxygen group, in which animals were subjected to osteotomy and 9 consecutive daily hyperbaric oxygen therapy sessions; and autologous platelet concentrate and hyperbaric oxygen group, in which animals were subjected to osteotomy, autologous platelet concentrate applied at the fracture site, and 9 consecutive daily hyperbaric oxygen therapy sessions. Each group was divided into 4 subgroups according to a pre-determined euthanasia time points: 2, 4, 6, and 8 weeks postoperative. After euthanasia at a specific time point, the fibula containing the osseous callus was prepared histologically and stained with hematoxylin and eosin or picrosirius red.
  
  
  RESULTS
  Autologous platelet concentrates and hyperbaric oxygen therapy, applied together or separately, increased the rate of bone healing compared with the control group.
  
  
  CONCLUSIONS
  Hyperbaric oxygen therapy and autologous platelet concentrate combined increased the rate of bone healing in this experimental model.
authors.affiliation: Colegiado de Medicina, Universidade Federal do Vale do São Francisco, PetrolinaPE, Brazil.
authors.forename: Paulo César Fagundes
authors.initials: PC
authors.lastname: Neves
authors.forename: Simone de Campos Vieira
authors.initials: Sde C
authors.lastname: Abib
authors.forename: Rogério Fagundes
authors.initials: RF
authors.lastname: Neves
authors.forename: Oronzo
authors.initials: O
authors.lastname: Pircchio
authors.forename: Karen Ruggeri
authors.initials: KR
authors.lastname: Saad
authors.forename: Paulo Fernandes
authors.initials: PF
authors.lastname: Saad
authors.forename: Ricardo Santos
authors.initials: RS
authors.lastname: Simões
authors.forename: Marcia Bento
authors.initials: MB
authors.lastname: Moreira
authors.forename: Cristiano Frota de Souza
authors.initials: CF
authors.lastname: Laurino
mesh_terms: D000818:Animals; D003094:Collagen / Q000032:analysis; D003131:Combined Modality Therapy / Q000379:methods; D004195:Disease Models, Animal; D005360:Fibula / Q000293:injuries*; D017102:Fracture Healing; D050723:Fractures, Bone / Q000503:physiopathology / Q000628:therapy*; D006931:Hyperbaric Oxygenation / Q000379:methods*; D008297:Male; D010027:Osteotomy; D053657:Platelet-Rich Plasma; D011817:Rabbits; D015203:Reproducibility of Results; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D003094:Collagen