title: Production of high-capacity adenovirus vectors.
abstract: High-capacity adenoviral vectors (HC-Ad), also known as "helper-dependent" (HD-Ad), "gutless", "gutted", or "third-generation" Ad vectors, are devoid of all viral coding sequences and have shown promising potential for a wide variety of different applications-from classic gene therapy to genetic vaccination and tumor treatment. However, compared to first-generation adenoviral vectors their production is more complex and requires specific in-depth knowledge. This chapter delivers a detailed protocol for the successful production of HC-Ad vectors to high titers.
authors.affiliation: Department of Gene Therapy, University of Ulm, Ulm, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Kreppel
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000302:isolation & purification*; D061249:Batch Cell Culture Techniques / Q000379:methods; D018929:Cell Culture Techniques; D005822:Genetic Vectors / Q000235:genetics / Q000302:isolation & purification* / Q000592:standards; D016679:Genome, Viral; D006378:Helper Viruses; D006801:Humans; D011786:Quality Control; D013048:Specimen Handling / Q000379:methods; D019562:Viral Load