title: [Real-time UV imaging of chloramphenicol intrinsic dissolution characteristics from ophthalmic in situ gel].
abstract: In this paper, chloramphenicol was selected as a model drug to prepare in situ gels. The intrinsic dissolution rate of chloramphenicol from in situ gel was evaluated using the surface dissolution imaging system. The results indicated that intrinsic dissolution rate of chloramphenicol thermosensitive in situ gel decreased significantly when the poloxamer concentration increased. The addition of the thickener reduced the intrinsic dissolution rate of chloramphenicol thermosensitive gel, wherein carbomer had the most impact. Different dilution ratios of simulated tear fluid greatly affected gel temperature, and had little influence on the intrinsic dissolution rate of chloramphenicol from the thermosensitive in situ gel. The pH of simulated tear fluid had little influence on the intrinsic dissolution rate of chloramphenicol thermosensitive in situ gel. For the pH sensitive in situ gel, the dissolution rates of chloramphenicol in weak acidic and neutral simulated tear fluids were slower than that in weak alkaline simulated tear fluid. In conclusion, the intrinsic dissolution of chloramphenicol from in situ gel was dependent on formulation and physiological factors. With advantages of small volume sample required and rapid detection, the UV imaging method can be an efficient tool for the evaluation of drug release characteristics of ophthalmic in situ gel.
authors.affiliation: Anhui University of Traditional Chinese Medicine, Hefei 230038, China.
authors.forename: Jian-Xiu
authors.initials: JX
authors.lastname: Chen
authors.forename: Zhen
authors.initials: Z
authors.lastname: Guo
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Wu
authors.forename: Zhong-Gui
authors.initials: ZG
authors.lastname: He
authors.forename: Rong-Feng
authors.initials: RF
authors.lastname: Hu
authors.forename: Ji-Wen
authors.initials: JW
authors.lastname: Zhang
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry*; D002701:Chloramphenicol / Q000008:administration & dosage / Q000737:chemistry*; D016503:Drug Delivery Systems; D005782:Gels / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009883:Ophthalmic Solutions / Q000737:chemistry*; D020442:Poloxamer / Q000737:chemistry; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D014783:Viscosity
chemical_list: D000180:Acrylic Resins; D000900:Anti-Bacterial Agents; D005782:Gels; D009883:Ophthalmic Solutions; C479038:carbomer; D020442:Poloxamer; D002701:Chloramphenicol