title: Fluorescence spectroscopy of soluble E. coli SPase I Δ2-75 reveals conformational changes in response to ligand binding.
abstract: The bacterial Sec pathway is responsible for the translocation of secretory preproteins. During the later stages of transport, the membrane-embedded signal peptidase I (SPase I) cleaves the signal peptide from a preprotein. We used tryptophan fluorescence spectroscopy of a soluble, catalytically active E. coli SPase I Δ2-75 enzyme to study its dynamic conformational changes while in solution and when interacting with lipids and signal peptides. We generated four single Trp SPase I Δ2-75 mutants, W261, W284, W300, and W310. Based on fluorescence quenching experiments, W300 and W310 were found to be more solvent accessible than W261 and W284 in the absence of ligands. W300 and W310 inserted into lipids, consistent with their location at the enzyme's proposed membrane-interface region, while the solvent accessibilities of W261, W284, and W300 were modified in the presence of signal peptide, suggesting propagation of structural changes beyond the active site in response to peptide binding. The signal peptide binding affinity for the enzyme was measured via FRET experiments and the Kd determined to be 4.4 μM. The location of the peptide with respect to the enzyme was also established; this positioning is crucial for the peptide to gain access to the enzyme active site as it emerges from the translocon into the membrane bilayer. These studies reveal enzymatic structural changes required for preprotein proteolysis as it interacts with its two key partners, the signal peptide and membrane phospholipids.
authors.affiliation: Department of Molecular and Cell Biology, The University of Connecticut, Storrs, Connecticut, 06269.
authors.forename: Meera K
authors.initials: MK
authors.lastname: Bhanu
authors.forename: Debra A
authors.initials: DA
authors.lastname: Kendall
mesh_terms: D020106:Acrylamide / Q000737:chemistry; D020134:Catalytic Domain / Q000502:physiology*; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D009154:Mutation; D010743:Phospholipids / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D021382:Protein Sorting Signals; D021381:Protein Transport; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000235:genetics; D015398:Signal Transduction; D013050:Spectrometry, Fluorescence
chemical_list: D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D010743:Phospholipids; D021382:Protein Sorting Signals; D020106:Acrylamide; D012697:Serine Endopeptidases; C026515:type I signal peptidase
keywords: acrylamide quenching; preprotein cleavage; protein transport; signal peptidase; signal peptide