title: Proteotyping of Holm oak (Quercus ilex subsp. ballota) provenances through proteomic analysis of acorn flour.
abstract: Proteomics has become a powerful tool to characterize biodiversity and natural variability in plant species, as well as to catalogue and establish phylogenetic relationships and distances among populations, provenances or ecotypes. In this chapter, we describe the standard proteomics workflow that we currently use in cataloguing Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) populations. Proteins are extracted from acorn flour or pollen by TCA/acetone or TCA/acetone-phenol methods, resolved by one- or two-dimensional gel electrophoresis, and gel images are captured and analyzed by appropriate software and statistical packages. Quantitative or qualitative variable bands or spots are subjected to MS analysis in order to identify them and correlate differences in the protein profile with the phenotypes or environmental conditions.
authors.affiliation: Department of Chemistry-Biology, Biomedical Sciences Institute, Autonomous University of Ciudad Juárez, Chihuahua, México.
authors.forename: José Valero
authors.initials: JV
authors.lastname: Galván
authors.forename: Raquel González
authors.initials: RG
authors.lastname: Fernández
authors.forename: Luis
authors.initials: L
authors.lastname: Valledor
authors.forename: Rafael Ma Navarro
authors.initials: RM
authors.lastname: Cerrillo
authors.forename: Jesus V
authors.initials: JV
authors.lastname: Jorrin-Novo
mesh_terms: D000096:Acetone / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D005433:Flour / Q000032:analysis*; D010802:Phylogeny; D010940:Plant Proteins / Q000302:isolation & purification; D011058:Pollen; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D029963:Quercus / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*; D013223:Statistics as Topic; D014238:Trichloroacetic Acid / Q000737:chemistry
chemical_list: D010940:Plant Proteins; D020543:Proteome; D000096:Acetone; D014238:Trichloroacetic Acid