title: miR-338-3p suppresses neuroblastoma proliferation, invasion and migration through targeting PREX2a.
abstract: MicroRNAs (miRNA) can regulate cancer cell proliferation and metastasis. Here, we show that miR-338-3p is down-regulated in metastatic tumor tissues compared to primary tumors, and that that miR-338-3p can inhibit cell proliferation by inducing cell cycle arrest, as well as restrain cell migration and invasion. PREX2a is confirmed as a direct target of miR-338-3p. Knockdown of PREX2a inhibits cell proliferation, migration and invasion through the PTEN/Akt pathway. miR-338-3p-dependent inhibition of proliferation and invasion can be rescued by PREXa. Overall, this study demonstrates that miR-338-3p affects the PTEN/Akt pathway by down-regulating PREX2a. This newly identified function of miR-338-3p provides novel insights into neuroblastoma and may foster therapeutic applications.
authors.affiliation: Department of Pediatric Surgery, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong, China. Electronic address: brabus-cx@163.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Pan
authors.forename: Lulu
authors.initials: L
authors.lastname: Han
authors.forename: Hongting
authors.initials: H
authors.lastname: Lu
authors.forename: Xiwei
authors.initials: X
authors.lastname: Hao
authors.forename: Qian
authors.initials: Q
authors.lastname: Dong
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009447:Neuroblastoma / Q000235:genetics / Q000378:metabolism* / Q000556:secondary; D051059:PTEN Phosphohydrolase / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D020413:3' Untranslated Regions; D020662:Guanine Nucleotide Exchange Factors; C532050:MIRN338 microRNA, human; D035683:MicroRNAs; C490712:PREX2 protein, human; D012333:RNA, Messenger; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: AKT; Metastasis; Microarray; Neuroblastoma; PREX2a; PTEN; miR-338-3p