title: MCM-41 solid phase membrane tip extraction combined with liquid chromatography for the determination of non-steroidal anti-inflammatory drugs in human urine.
abstract: Mesoporous silica material, MCM-41, was utilized for the first time as an adsorbent in solid phase membrane tip extraction (SPMTE) of non-steroidal anti-inflammatory drugs (NSAIDs) in urine prior to high performance liquid chromatography-ultraviolet (HPLC-UV) analysis. The prepared MCM-41 material was enclosed in a polypropylene membrane tip and used as an adsorbent in SPMTE. Four NSAIDs namely ketoprofen, diclofenac, mefenamic acid and naproxen were selected as model analytes. Several important parameters, such as conditioning solvent, sample pH, salting-out effect, sample volume, extraction time, desorption solvent and desorption time were optimized. Under the optimum extraction conditions, the MCM-41-SPMTE method showed good linearity in the range of 0.01-10μg/mL with excellent correlation coefficients (r=0.9977-0.9995), acceptable RSDs (0.4-9.4%, n=3), good limits of detection (5.7-10.6μg/L) and relative recoveries (81.4-108.1%). The developed method showed a good tolerance to biological sample matrices.
authors.affiliation: Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia; Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Sazlinda
authors.initials: S
authors.lastname: Kamaruzaman
authors.forename: Mohd Marsin
authors.initials: MM
authors.lastname: Sanagi
authors.forename: Salasiah
authors.initials: S
authors.lastname: Endud
authors.forename: Wan Aini
authors.initials: WA
authors.lastname: Wan Ibrahim
authors.forename: Noorfatimah
authors.initials: N
authors.lastname: Yahaya
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D012997:Solvents / Q000737:chemistry
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; C509968:MCM-41; D012997:Solvents; D012965:Sodium Chloride; D012822:Silicon Dioxide
keywords: Mesoporous silica; Non-steroidal anti-inflammatory drugs; Solid phase membrane tip extraction; Urine