title: Paraoxonase and arylesterase activity and total oxidative/anti-oxidative status in patients with idiopathic Parkinson's disease.
abstract: This study investigated serum paraoxonase (PON1) and arylesterase activity along with determination of oxidative status via measurement of total oxidant status (TOS), total anti-oxidant status (TAS) and oxidative stress index (OSI) in patients with Parkinson's disease (PD) and compared results with data from healthy controls. A total of 82 subjects, including 42 patients with idiopathic PD, newly diagnosed and untreated (24 men, 18 women, aged 47-66 years) and 40 healthy controls were enrolled in this study. We aimed to evaluate the oxidative status of PD patients via measurement of serum TOS and TAS and estimation of OSI using new automated methods. PON1 and arylesterase activities were measured spectrophotometrically. Serum total cholesterol, high density lipoprotein cholesterol, low density lipoprotein (LDL) cholesterol and triglyceride levels were measured using routine methods. TAS levels of PD patients were significantly lower than that of controls (p<0.05). TOS levels of PD patients were higher than those of controls (p<0.05). PON1 and arylesterase activities of PD were lower than those of controls (p<0.05). Serum levels of total and LDL cholesterol were significantly reduced in PD patients. In conclusion, the presence of high TOS and OSI levels together with low levels of TAS in PD patients supports the important role of oxidative stress in the pathophysiology of PD. Since oxidative stress is involved in neurodegeneration, selecting anti-oxidants, metal chelators or other compounds boosting endogenous enzymatic and non-enzymatic defense mechanisms seems to be an obvious choice as treatment for PD.
authors.affiliation: Department of Biochemistry, Recep Tayyip Erdoğan University Faculty of Medicine, İslampaşa mah., Merkez/Rize 53100, Turkey. Electronic address: aynur.kirbas@erdogan.edu.tr.
authors.forename: Aynur
authors.initials: A
authors.lastname: Kirbas
authors.affiliation: Department of Neurology, Recep Tayyip Erdoğan University Faculty of Medicine, Rize, Turkey.
authors.forename: Serkan
authors.initials: S
authors.lastname: Kirbas
authors.affiliation: Department of Biochemistry, Recep Tayyip Erdoğan University Faculty of Medicine, İslampaşa mah., Merkez/Rize 53100, Turkey.
authors.forename: Medine Cumhur
authors.initials: MC
authors.lastname: Cure
authors.affiliation: Department of Neurology, Recep Tayyip Erdoğan University Faculty of Medicine, Rize, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Tufekci
mesh_terms: D043303:Aryldialkylphosphatase / Q000097:blood*; D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D010300:Parkinson Disease / Q000378:metabolism*
chemical_list: D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase
keywords: Arylesterase; Oxidative stress index; Paraoxonase; Parkinson’s disease; Total anti-oxidant status; Total oxidant status