title: [Diammonium glycyrrhizinate inhibits airway smooth muscle proliferation in a murine model of chronic asthma].
abstract: OBJECTIVE
  To investigate the therapeutic value and possible mechanism of diammonium glycyrrhizinate (DG) in treatment of airway remodeling in a murine model of chronic asthma.
  
  
  METHODS
  Thirty male BALB/C mice were randomly divided into control group, OVA+DG group and OVA group (n=10). HE staining was used to observe the pathological changes, and Masson's staining was used to detect and measure collagen deposition. Alpha-SMA and PPARγ mRNA expressions were analyzed by RT-PCR, and the protein expressions of α-SMA and PPARγ were measured by Western blotting.
  
  
  RESULTS
  After 75 days of OVA sensitization and challenge, obvious pathological changes occurred in the lung tissues, which was more severe in OVA group than in OVA+DG group. Collagen deposition was significantly increased after OVA stimulation, but was obviously milder in OVA+DG group than in OVA group. OVA-induced up-regulation of α-SMA was notably attenuated by DG injection. The expression of PPARγ was markedly down-regulated after OVA stimulation but was substantially enhanced after DG intervention.
  
  
  CONCLUSIONS
  DG can inhibit airway smooth muscle proliferation possibly through up-regulation of PPARγ in a murine model of chronic asthma.
authors.affiliation: Division of Pulmonary Diseases, State Key Laboratory of Biotherapy of China/Department of Respiratory Medicine, West China Hospital of Sichuan University, Chengdu 610041, China. E-mail: xiaomin_wcms@163.com.
authors.forename: Min
authors.initials: M
authors.lastname: Xiao
authors.forename: Tao
authors.initials: T
authors.lastname: Zhu
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Wen
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D056151:Airway Remodeling / Q000187:drug effects; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001249:Asthma / Q000378:metabolism / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects*; D003094:Collagen / Q000378:metabolism; D019695:Glycyrrhizic Acid / Q000494:pharmacology*; D008168:Lung / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009130:Muscle, Smooth / Q000473:pathology*; D010047:Ovalbumin / Q000494:pharmacology; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D015854:Up-Regulation
chemical_list: D000199:Actins; D000893:Anti-Inflammatory Agents; D047495:PPAR gamma; D012333:RNA, Messenger; C544013:alpha-smooth muscle actin, mouse; D019695:Glycyrrhizic Acid; D010047:Ovalbumin; D003094:Collagen