title: Development of a capillary electrophoresis-based heteroduplex tracking assay to measure in-host genetic diversity of initial and recurrent Plasmodium vivax infections in Cambodia.
abstract: A heteroduplex tracking assay used to genotype Plasmodium vivax merozoite surface protein 1 was adapted to a capillary electrophoresis format, obviating the need for radiolabeled probes and allowing its use in settings where malaria is endemic. This new assay achieved good allelic discrimination and detected high multiplicities of infection in 63 P. vivax infections in Cambodia. More than half of the recurrent parasitemias sampled displayed identical or highly related genotypes compared to the initial genotype, suggesting that they represented relapses.
authors.affiliation: Division of Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA.
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Givens
authors.forename: Jessica T
authors.initials: JT
authors.lastname: Lin
authors.forename: Chanthap
authors.initials: C
authors.lastname: Lon
authors.forename: Panita
authors.initials: P
authors.lastname: Gosi
authors.forename: Meng Chuor
authors.initials: MC
authors.lastname: Char
authors.forename: Charlotte A
authors.initials: CA
authors.lastname: Lanteri
authors.forename: David L
authors.initials: DL
authors.lastname: Saunders
authors.forename: Jonathan J
authors.initials: JJ
authors.lastname: Juliano
mesh_terms: D002160:Cambodia; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D019075:Electrophoresis, Capillary / Q000379:methods*; D014644:Genetic Variation; D020180:Heteroduplex Analysis / Q000379:methods*; D006801:Humans; D016780:Malaria, Vivax / Q000469:parasitology*; D020066:Merozoite Surface Protein 1 / Q000235:genetics*; D008969:Molecular Sequence Data; D010966:Plasmodium vivax / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012008:Recurrence; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan; D020066:Merozoite Surface Protein 1