title: Long-term biopermanence of ceramides, cholesteryl esters, and ether-linked triglycerides with very-long-chain PUFA in the cadmium-damaged testis.
abstract: Cadmium is known to harm rat testis by causing the dose-dependent apoptotic or necrotic death of seminiferous epithelium cells. Here we investigated how this affects the lipids with long-chain (C18-C22) and very-long-chain (C24-C32) polyunsaturated fatty acids (VLCPUFA) typical of spermatogenic and Sertoli cells. A severe acute inflammatory reaction resulted from the massive necrotic death of these cells two days after a single high (4mg/kg) dose of CdCl2. This led to the conversion of most testicular glycerophospholipids to diradylglycerols (DRG) and free fatty acids (FFA) and of most sphingomyelins to ceramides (Cer). By day 30 the testis weight had decreased three-fold. The DRG and FFA had been metabolized but, unexpectedly, ceramides persisted. Also slow to disappear were VLCPUFA-containing triacylglycerols from former germ cells and ether-linked triglycerides and cholesteryl esters (CE) from former Sertoli cells. Similar results were observed 30 and 45days after administering repeated small non pro-inflammatory CdCl2 doses (1mg/kg). At day 30 after both treatments, an amorphous material replaced the original seminiferous tubules and the interstitium was populated by macrophages. Species of CE and ether-linked triglycerides containing fatty acids other than VLCPUFA steadily accumulated in the irreversibly damaged testis, a manifestation of the activity of these cells. The long-term permanence of original VLCPUFA-containing neutral lipids, especially ceramides, indicates that these phagocytes were slow to clear out the acellular material contained in seminiferous tubules, pointing to a form of silent chronic inflammation as an additional outcome of the multifactorial commotion caused in the testis by experimentally administered cadmium.
authors.affiliation: Instituto de Investigaciones Bioquímicas de Bahía Blanca, Consejo Nacional de Investigaciones Científicas y Técnicas, and Universidad Nacional del Sur, 8000 Bahía Blanca, Argentina.
authors.forename: Samanta R
authors.initials: SR
authors.lastname: Zanetti
authors.forename: Marta I
authors.initials: MI
authors.lastname: Aveldaño
mesh_terms: D000818:Animals; D002104:Cadmium / Q000633:toxicity; D019256:Cadmium Chloride / Q000633:toxicity*; D002518:Ceramides / Q000378:metabolism*; D002788:Cholesterol Esters; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects*; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008297:Male; D009336:Necrosis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012671:Seminiferous Tubules / Q000378:metabolism* / Q000473:pathology; D012708:Sertoli Cells / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D002518:Ceramides; D002788:Cholesterol Esters; D005231:Fatty Acids, Unsaturated; D014280:Triglycerides; D002104:Cadmium; D019256:Cadmium Chloride
keywords: Germ cells; Macrophages; Sertoli cells; Sphingomyelins; Triacylglycerols; Very long chain fatty acids