title: Integrin CD11c/CD18 α-chain phosphorylation is functionally important.
abstract: CD11c/CD18 (αXβ2, p150/95, or complement receptor 4, CR4) is a monocyte/macrophage-enriched integrin that has been reported to bind to a variety of ligands. These include cell surface proteins, extracellular matrix proteins, and soluble ligands. The regulation of ligand binding to CD11c/CD18 has remained poorly understood. Previous work has shown that both α-chain and β-chain phosphorylations of CD11a/CD18 and CD11b/CD18 are needed for activity, but no corresponding studies on CD11c/CD18 have been performed. In this study, we have identified the phosphorylation site of CD11c as Ser-1158 and show that it is pivotal for adherence and phagocytosis.
authors.affiliation: From the Division of Biochemistry and Biotechnology, Department of Biosciences, University of Helsinki, 00014 Helsinki, Finland.
authors.forename: Liisa M
authors.initials: LM
authors.lastname: Uotila
authors.forename: Maria
authors.initials: M
authors.lastname: Aatonen
authors.forename: Carl G
authors.initials: CG
authors.lastname: Gahmberg
mesh_terms: D000818:Animals; D039521:CD11c Antigen / Q000235:genetics / Q000378:metabolism*; D018821:CD18 Antigens / Q000235:genetics / Q000378:metabolism*; D019556:COS Cells; D002448:Cell Adhesion / Q000502:physiology; D002522:Chlorocebus aethiops; D006801:Humans; D020014:K562 Cells; D010587:Phagocytosis / Q000502:physiology*; D010766:Phosphorylation / Q000502:physiology
chemical_list: D039521:CD11c Antigen; D018821:CD18 Antigens
keywords: Adhesion; Integrins; Leukocyte; Phagocytosis; Phosphorylation