title: Zebrafish brain proteomics reveals central proteins involved in neurodegeneration.
abstract: Understanding the complex biology of the brain requires analyzing its structural and functional complexity at the protein level. The large-scale analysis of the brain proteome, coupled with characterization of central brain proteins, provides insight into fundamental brain processes and processes linked to neurodegenerative diseases. Here we provide a map of the zebrafish brain proteome by using two-dimensional gel electrophoresis (2DE), followed by the identification of 95 brain proteins using mass spectrometry (LC-ESI MS/MS). Our data show extensive phosphorylation of brain proteins but less prominent glycosylation. Furthermore, ~51% of the identified proteins are predicted to have one or more ubiquitination sites whereas ~90% are predicted to have one or more SUMOylation sites. Our findings provide a valuable proteome map of the zebrafish brain and associated posttranslational modifications demonstrating that zebrafish proteomic approaches can aid in our understanding of proteins central to important neuronal processes and those associated with neurodegenerative disorders.
authors.affiliation: Centre for Organelle Research, University of Stavanger, Stavanger, Norway.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Gebriel
authors.forename: Shubhangi
authors.initials: S
authors.lastname: Prabhudesai
authors.forename: Kai-Erik
authors.initials: KE
authors.lastname: Uleberg
authors.forename: Eivind
authors.initials: E
authors.lastname: Larssen
authors.forename: Dominik
authors.initials: D
authors.lastname: Piston
authors.forename: Anne Hjelle
authors.initials: AH
authors.lastname: Bjørnstad
authors.forename: Simon Geir
authors.initials: SG
authors.lastname: Møller
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006031:Glycosylation; D009410:Nerve Degeneration / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D053719:Tandem Mass Spectrometry; D015027:Zebrafish / Q000235:genetics / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D020543:Proteome; D029961:Zebrafish Proteins
keywords: brain; neurodegeneration; proteomics; zebrafish