title: Association between UCP3 gene polymorphisms and nonalcoholic fatty liver disease in Chinese children.
abstract: OBJECTIVE
  To confirm the hypothesis that polymorphisms of the uncoupling protein 3 (UCP3) gene are associated with the occurrence of nonalcoholic fatty liver disease (NAFLD).
  
  
  METHODS
  A total of 250 NAFLD patients (147 males and 103 females) and 200 healthy individuals who served as controls (control, 109 males and 91 females), aged between 6 and 16 years were enrolled in this study. The four non-synonymous single nucleotide polymorphisms (SNPs) in the UCP3 gene polymorphisms of rs1726745, rs3781907, rs11235972 and rs1800849, were genotyped using MassArray. Body mass index (BMI), waist and hip circumference, blood pressure (BP), fasting blood glucose (FBG), insulin and lipid profiles were measured and B-ultrasound examination was performed in all subjects.
  
  
  RESULTS
  NAFLD patients showed risk factors for metabolic syndrome: elevated BMI, waist-to-hip ratio, BP, FBG, homeostasis model assessment-estimated insulin resistance, total triglyceride, total cholesterol and low-density lipoprotein-cholesterol, while decreased high-density lipoprotein-cholesterol level compared with the control group. The GG genotype distributions of rs11235972 in the NAFLD group differed significantly from that in the control group. We found that waist circumference between CC (58.76 ± 6.45 cm) and CT+TT (57.00 ± 5.59 cm), and hip circumference between CC (71.28 ± 7.84 cm) and CT+TT genotypes (69.06 ± 7.75 cm) were significantly different with and without rs1800849 variation (P < 0.05).
  
  
  CONCLUSIONS
  A higher prevalence of rs11235972 GG genotype was observed in the NAFLD group compared with the control group. No differences were observed for the other SNPs. However, there was a significant difference in body height in addition to waist and hip circumference between the CC (mutant type group) and CT+TT group with and without rs1800849 variation.
authors.affiliation: Yan-Ping Xu, Jun-Fen Fu, Department of Endocrinology, Children's Hospital of Zhejiang University School of Medicine, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Xu
authors.forename: Li
authors.initials: L
authors.lastname: Liang
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Wang
authors.forename: Jun-Fen
authors.initials: JF
authors.lastname: Fu
authors.forename: Pei-Ning
authors.initials: PN
authors.lastname: Liu
authors.forename: Lan-Qiu
authors.initials: LQ
authors.lastname: Lv
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: Zhu
mesh_terms: D000293:Adolescent; D000367:Age Factors; D044466:Asian People / Q000235:genetics*; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000032:analysis; D001794:Blood Pressure; D015992:Body Mass Index; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002648:Child; D002681:China / Q000453:epidemiology; D005234:Fatty Liver / Q000097:blood / Q000175:diagnosis / Q000208:ethnology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D007328:Insulin / Q000097:blood; D007473:Ion Channels / Q000235:genetics*; D008055:Lipids / Q000097:blood; D016015:Logistic Models; D008297:Male; D024101:Mitochondrial Proteins / Q000235:genetics*; D015999:Multivariate Analysis; D065626:Non-alcoholic Fatty Liver Disease; D016017:Odds Ratio; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D000071247:Uncoupling Protein 3; D049629:Waist-Hip Ratio
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D007328:Insulin; D007473:Ion Channels; D008055:Lipids; D024101:Mitochondrial Proteins; C000604392:UCP3 protein, human; D000071247:Uncoupling Protein 3
keywords: Nonalcoholic fatty liver disease; Single nucleotide polymorphisms; Uncoupling protein 3