title: Rapid determination of quetiapine in blood by gas chromatography-mass spectrometry. Application to post-mortem cases.
abstract: A simple, fast and sensitive method for the determination of quetiapine in human blood has been developed and validated. The method involved a basic liquid-liquid extraction procedure and subsequent analysis by gas chromatography-mass spectrometry, previous derivatization with bis(trimethylsilyl)-trifluoro-acetamide and chorotrimethylsilane (99 : 1). The methods of validation included linearity with a correlation coefficient > 0.99 over the range 0.02-1 µg ml(-1), intra- and interday precision (always < 12%) and accuracy (mean relative error always < 12%) to meet the bioanalytical acceptance criteria. The limit of detection was 0.005 µg ml(-1). The procedure was further applied to post mortems from the Institute of Legal Medicine, University of Santiago de Compostela.
authors.affiliation: Department of Legal Medicine and Toxicology, University of Granada School of Medicine, Avda. Madrid, 11, 18071, Granada, Spain.
authors.forename: Olga
authors.initials: O
authors.lastname: López-Guarnido
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Tabernero
authors.forename: Antonio F
authors.initials: AF
authors.lastname: Hernández
authors.forename: Lourdes
authors.initials: L
authors.lastname: Rodrigo
authors.forename: Ana M
authors.initials: AM
authors.lastname: Bermejo
mesh_terms: D000328:Adult; D000368:Aged; D014150:Antipsychotic Agents / Q000097:blood* / Q000506:poisoning; D001344:Autopsy / Q000379:methods*; D002138:Calibration; D003987:Dibenzothiazepines / Q000097:blood* / Q000506:poisoning; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006197:Hair / Q000737:chemistry; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D008875:Middle Aged; D000069348:Quetiapine Fumarate; D015203:Reproducibility of Results
chemical_list: D014150:Antipsychotic Agents; D003987:Dibenzothiazepines; D007202:Indicators and Reagents; D000069348:Quetiapine Fumarate
keywords: GC/MS; blood analysis; forensic toxicology; liquid-liquid extraction; quetiapine