title: Nanofibers implant functionalized by neural growth factor as a strategy to innervate a bioengineered tooth.
abstract: Current strategies for jaw reconstruction require multiple procedures, to repair the bone defect, to offer sufficient support, and to place the tooth implant. The entire procedure can be painful and time-consuming, and the desired functional repair can be achieved only when both steps are successful. The ability to engineer combined tooth and bone constructs, which would grow in a coordinated fashion with the surrounding tissues, could potentially improve the clinical outcomes and also reduce patient suffering. A unique nanofibrous and active implant for bone-tooth unit regeneration and also the innervation of this bioengineered tooth are demonstrated. A nanofibrous polycaprolactone membrane is functionalized with neural growth factor, along with dental germ, and tooth innervation follows. Such innervation allows complete functionality and tissue homeostasis of the tooth, such as dentinal sensitivity, odontoblast function, masticatory forces, and blood flow.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM), UMR 1109, Osteoarticular and Dental Regenerative Nanomedicine, Faculté de Médecine, 67085, Strasbourg Cedex, France; Université de Strasbourg Faculté de Chirurgie Dentaire, 1 place de l'Hôpital, 67000, Strasbourg, France.
authors.forename: Sandy
authors.initials: S
authors.lastname: Eap
authors.forename: Thibault
authors.initials: T
authors.lastname: Bécavin
authors.forename: Laetitia
authors.initials: L
authors.lastname: Keller
authors.forename: Tunay
authors.initials: T
authors.lastname: Kökten
authors.forename: Florence
authors.initials: F
authors.lastname: Fioretti
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Weickert
authors.forename: Etienne
authors.initials: E
authors.lastname: Deveaux
authors.forename: Nadia
authors.initials: N
authors.lastname: Benkirane-Jessel
authors.forename: Sabine
authors.initials: S
authors.lastname: Kuchler-Bopp
mesh_terms: D000818:Animals; D001698:Biomedical Engineering / Q000379:methods; D001861:Bone Regeneration; D015921:Dental Implants; D019248:Jaw Fixation Techniques / Q000295:instrumentation*; D051379:Mice; D008813:Mice, Inbred ICR; D057139:Nanofibers / Q000737:chemistry*; D020932:Nerve Growth Factor / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014070:Tooth / Q000737:chemistry*
chemical_list: D015921:Dental Implants; D011091:Polyesters; C016240:polycaprolactone; D020932:Nerve Growth Factor
keywords: growth factors; innervation; macrostructured materials; nanomaterials; tissue engineering; tooth regeneration