title: Biodegradation of a mixture of PAHs by non-ligninolytic fungal strains isolated from crude oil-contaminated soil.
abstract: Nine native non-ligninolytic fungal strains were isolated from Maya crude oil-contaminated soil and selected based on their ability to grow and use crude oil and several polycyclic aromatic hydrocarbons (PAHs) as carbon source, for their application to PAH removal in soil. The fungi were identified by PCR amplification of intergenic transcribed sequences regions and microbiological techniques, and results showed them to be part of the genera Fusarium, Neurospora, Aspergillus, Scedosporium, Penicillium, Neosartorya and Talaromyces. A primary selection of fungi was made in minimal medium plates, considering the tolerance to different concentrations of PAHs for each strain. The radial extension rate exhibited significant differences (p < 0.05) from 200 to 1,000 mg of PAHs mixture l⁻¹. A secondary selection of Aspergillus terreus, Talaromyces spectabilis, and Fusarium sp. was achieved based on their tolerance to 2,000 mg of a mixture of Phenanathrene and Pyrene kg⁻¹ of soil in a solid-state microcosm system for 2 weeks. The percentage of PAH removal obtained by the three strains was approximately 21 % of the mixture.
authors.affiliation: Instituto Politécnico Nacional, Centro de Investigación en Biotecnología Aplicada, Carretera Federal Santa Inés Tecuexcomac-Tepetitla Km 1.5, C.P. 90700, Tepetitla de Lardizabal, Tlaxcala, Mexico.
authors.forename: Anaisell
authors.initials: A
authors.lastname: Reyes-César
authors.forename: Ángel E
authors.initials: ÁE
authors.lastname: Absalón
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Fernández
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: González
authors.forename: Diana V
authors.initials: DV
authors.lastname: Cortés-Espinosa
mesh_terms: D001711:Biotransformation; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D016533:Mycological Typing Techniques; D010578:Petroleum / Q000378:metabolism*; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants