title: Novel tripod amphiphiles for membrane protein analysis.
abstract: Integral membrane proteins play central roles in controlling the flow of information and molecules across membranes. Our understanding of membrane protein structures and functions, however, is seriously limited, mainly due to difficulties in handling and analysing these proteins in aqueous solution. The use of a detergent or other amphipathic agents is required to overcome the intrinsic incompatibility between the large lipophilic surfaces displayed by the membrane proteins in their native forms and the polar solvent molecules. Here, we introduce new tripod amphiphiles displaying favourable behaviours toward several membrane protein systems, leading to an enhanced protein solubilisation and stabilisation compared to both conventional detergents and previously described tripod amphiphiles.
authors.affiliation: Department of Bionano Engineering, Hanyang University Ansan, 426-791 (Korea), Fax: (+81) 31-436-8146. pchae@hanyang.ac.kr.
authors.forename: Pil Seok
authors.initials: PS
authors.lastname: Chae
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Kruse
authors.forename: Kamil
authors.initials: K
authors.lastname: Gotfryd
authors.forename: Rohini R
authors.initials: RR
authors.lastname: Rana
authors.forename: Kyung Ho
authors.initials: KH
authors.lastname: Cho
authors.forename: Søren G F
authors.initials: SG
authors.lastname: Rasmussen
authors.forename: Hyoung Eun
authors.initials: HE
authors.lastname: Bae
authors.forename: Richa
authors.initials: R
authors.lastname: Chandra
authors.forename: Ulrik
authors.initials: U
authors.lastname: Gether
authors.forename: Lan
authors.initials: L
authors.lastname: Guan
authors.forename: Brian K
authors.initials: BK
authors.lastname: Kobilka
authors.forename: Claus J
authors.initials: CJ
authors.lastname: Loland
authors.forename: Bernadette
authors.initials: B
authors.lastname: Byrne
authors.forename: Samuel H
authors.initials: SH
authors.lastname: Gellman
mesh_terms: D003902:Detergents / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry; D012995:Solubility; D012997:Solvents / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D003902:Detergents; D008565:Membrane Proteins; D012997:Solvents; D013501:Surface-Active Agents
keywords: amphiphiles; membrane proteins; molecular design; protein structures; stabilization