title: The role of Atg29 phosphorylation in PAS assembly.
abstract: Macroautophagy (hereafter autophagy) initiates at the phagophore assembly site (PAS), where most of the AuTophaGy-related (Atg) proteins are at least transiently localized. As the first protein complex targeted to the PAS, the Atg17-Atg31-Atg29 complex serves as the scaffold for other Atg proteins and plays a critical role for the organization of the PAS, and in autophagy initiation. We recently showed that this complex is constitutively formed and activated by the phosphorylation of Atg29 when autophagy is induced. Phosphorylation of Atg29 is required for its interaction with Atg11, another scaffold protein, and its function for promoting the proper assembly of the PAS. Single-particle electron microscopy analysis of the Atg17-Atg31-Atg29 complex reveals an elongated structure with Atg29 located at the opposing ends. This structural arrangement allows Atg29 to interact with Atg11, and is critical in the organization of the intact Atg1 complex.
authors.affiliation: Life Sciences Institute; University of Michigan; Ann Arbor, MI USA.
authors.forename: Kai
authors.initials: K
authors.lastname: Mao
authors.forename: Leon H
authors.initials: LH
authors.lastname: Chew
authors.forename: Calvin K
authors.initials: CK
authors.lastname: Yip
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Klionsky
mesh_terms: D000595:Amino Acid Sequence; D001343:Autophagy; D000071183:Autophagy-Related Proteins; D002352:Carrier Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D030781:Organisms, Genetically Modified; D010588:Phagosomes / Q000378:metabolism*; D010766:Phosphorylation; D054730:Protein Interaction Domains and Motifs; D011494:Protein Kinases / Q000378:metabolism; D055503:Protein Multimerization; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000378:metabolism
chemical_list: C506770:ATG29 protein, S cerevisiae; C502923:Atg11 protein, S cerevisiae; C505602:Atg17 protein, S cerevisiae; C520043:Atg31 protein, S cerevisiae; D000071183:Autophagy-Related Proteins; D002352:Carrier Proteins; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins; D011494:Protein Kinases; C477823:ATG1 protein, S cerevisiae
keywords: PAS; autophagy; scaffold; vacuole; yeast