title: Development of pneumococcal polysaccharide conjugate vaccine with long spacer arm.
abstract: Streptococcus pneumoniae is a serious Gram-positive pathogen responsible for several life-threatening pneumococcal diseases. Pneumococcal capsular polysaccharide (CPS) is a key virulence determinant of S. pneumoniae and its immunogenicity can be improved by conjugation with a carrier protein. Reductive amination, the most widely used approach for pneumococcal CPS conjugate vaccine (PCV), suffers from low conjugation efficiency and the problem of steric hindrance. Here, copper-catalyzed azide-alkyne cycloaddition was used for development of PCV with long spacer arm (L-PCV). Tetanus toxoid (TT) was used as the carrier protein. The long spacer arm in L-PCV can minimize the problem of steric hindrance between CPS and TT, thereby improving the CPS-specific antibody titers in the mice model. L-PCV can also induce high avidity functional antibody and elicit immunological memory in response to the native CPS.
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, No. 1 Bei-Er-Tiao Street, Haidian District, Beijing 100190, China; College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China.
authors.forename: Dinglong
authors.initials: D
authors.lastname: Wu
authors.forename: Shaoyang
authors.initials: S
authors.lastname: Ji
authors.forename: Tao
authors.initials: T
authors.lastname: Hu
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D022242:Pneumococcal Vaccines / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D013296:Streptococcus pneumoniae / Q000276:immunology*; D013745:Tetanus Toxoid / Q000008:administration & dosage* / Q000737:chemistry; D018074:Vaccines, Conjugate / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000907:Antibodies, Bacterial; D004337:Drug Carriers; D022242:Pneumococcal Vaccines; D011135:Polysaccharides, Bacterial; D013745:Tetanus Toxoid; D018074:Vaccines, Conjugate
keywords: Azide-alkyne cycloaddition; Capsular polysaccharide; Conjugate vaccine; Streptococcus pneumoniae