title: A nanopore-nanofiber mesh biosensor to control DNA translocation.
abstract: Solid-state nanopores show promise as single-molecule sensors for biomedical applications, but to increase their resolution and efficiency, analyte molecules must remain longer in the nanopore sensing volume. Here we demonstrate a novel, facile, and customizable nanopore sensor modification that reduces the double-stranded DNA translocation velocity by 2 orders of magnitude or more via interactions outside the nanopore. This is achieved by electrospinning a copolymer nanofiber mesh (NFM) directly onto a solid-state nanopore (NP) chip. The effect of NFMs on dsDNA translocation through an NP is highlighted using a set of NFMs of varying mesh composition that reduce the translocation speed relative to a bare pore from 1- to >100-fold. A representative NFM from this set is effective on DNA as long as 20 kbp, improves the nanopore resolution, and allows discrimination among different DNA lengths.
authors.forename: Allison H
authors.initials: AH
authors.lastname: Squires
authors.forename: Joseph S
authors.initials: JS
authors.lastname: Hersey
authors.forename: Mark W
authors.initials: MW
authors.lastname: Grinstaff
authors.forename: Amit
authors.initials: A
authors.lastname: Meller
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D057139:Nanofibers / Q000737:chemistry*; D058608:Nanopores
chemical_list: D004247:DNA