title: Efficacy of bacteriophage LISTEX™P100 combined with chemical antimicrobials in reducing Listeria monocytogenes in cooked turkey and roast beef.
abstract: The aim of this study was to verify the effectiveness of the commercially available anti-Listeria phage preparation LISTEX(™)P100 in reducing Listeria monocytogenes on ready-to-eat (RTE) roast beef and cooked turkey in the presence or absence of the chemical antimicrobials potassium lactate (PL) and sodium diacetate (SD). Sliced RTE meat cores at 4 and 10 °C were inoculated with cold-adapted L. monocytogenes to result in a surface contamination level of 10(3)CFU/cm(2). LISTEX(TM)P100 was applied at 10(7) PFU/cm(2) and samples taken at regular time intervals during the RTE product's shelf life to enumerate viable L. monocytogenes. LISTEX(™)P100 was effective during incubation at 4 °C with initial reductions of L. monocytogenes of 2.1 log10 CFU/cm(2) and 1.7 log10 CFU/cm(2), respectively, for cooked turkey and roast beef without chemical antimicrobials (there was no significant difference to the initial L. monocytogenes reductions in the presence of LISTEX(TM)P100 for cooked turkey containing PL and roast beef containing SD-PL). In the samples containing no chemical antimicrobials, the presence of phage resulted in lower L. monocytogenes numbers, relative to the untreated control, of about 2 log CFU/cm(2) over a 28-day storage period at 4 °C. An initial L. monocytogenes cell reduction of 1.5 log10 CFU/cm(2) and 1.7 log10 CFU/cm(2), respectively, for cooked turkey and roast beef containing no chemical antimicrobials was achieved by the phage at 10 °C (abusive temperature). At this temperature, the L. monocytogenes cell numbers of samples treated with LISTEX™ P100 remained below those of the untreated control only during the first 14 days of the experiment for roast beef samples with and without antimicrobials. On day 28, the L. monocytogenes numbers on samples containing chemical antimicrobials and treated with LISTEX(TM)P100 stored at 4 and 10 °C were 4.5 log10 CFU/cm(2) and 7.5 log10 CFU/cm(2), respectively, for cooked turkey, and 1.2 log10 CFU/cm(2) and 7.2 log10 CFU/cm(2), respectively, for roast beef. In both cooked turkey samples with and without chemical antimicrobials stored at 10 °C, the phage-treated samples had significantly lower numbers of L. monocytogenes when compared to the untreated controls throughout the 28-day storage period (P<0.0001). For roast beef and cooked turkey containing chemical antimicrobials treated with LISTEX(TM)P100 and stored at 4 °C, no more than a 2 log CFU/cm(2) increase of L. monocytogenes was observed throughout the stated shelf life of the product. This study shows that LISTEX(™)P100 causes an initial reduction of L. monocytogenes numbers and can serve as an additional hurdle to enhance the safety of RTE meats when used in combination with chemical antimicrobials.
authors.affiliation: Guelph Food Research Centre, Agriculture and Agri-Food Canada, 93 Stone Road West, Guelph, Ontario, N1G 5C9, Canada; Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, 110 Stone Road West, Guelph, Ontario, N1G 3W4, Canada.
authors.forename: Andrew
authors.initials: A
authors.lastname: Chibeu
authors.forename: Louise
authors.initials: L
authors.lastname: Agius
authors.forename: Anli
authors.initials: A
authors.lastname: Gao
authors.forename: Parviz M
authors.initials: PM
authors.lastname: Sabour
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Kropinski
authors.forename: S
authors.initials: S
authors.lastname: Balamurugan
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D001435:Bacteriophages; D002417:Cattle; D015169:Colony Count, Microbial; D003296:Cooking; D059022:Food Safety; D061353:Food Storage; D019344:Lactic Acid / Q000494:pharmacology; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D008461:Meat Products / Q000382:microbiology*; D013696:Temperature; D014422:Turkeys
chemical_list: D000085:Acetates; D000890:Anti-Infective Agents; C548838:sodium diacetate; D019344:Lactic Acid
keywords: Bacteriophage; LISTEX(™)P100; Listeria monocytogenes; Potassium lactate; Sodium diacetate