title: Stereocomplementary bioreduction of β-ketonitrile without ethylated byproduct.
abstract: α-Ethylation is competing with the biocatalytic reduction of aromatic β-ketonitriles in a whole-cell system. Use of two newly mined robust and stereocomplementary carbonyl reductases in a biphasic system has completely eliminated the competing byproduct. For the first time, both enantiomers of fluoroxetine precursors were obtained at 0.5 M with >99% ee and excellent chemoselectivity, without addition of any external cofactors.
authors.affiliation: Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , 130 Meilong Road, Shanghai 200237, People's Republic of China.
authors.forename: Guo-Chao
authors.initials: GC
authors.lastname: Xu
authors.forename: Hui-Lei
authors.initials: HL
authors.lastname: Yu
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Zhang
authors.forename: Jian-He
authors.initials: JH
authors.lastname: Xu
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D055162:Biocatalysis; D007659:Ketones / Q000737:chemistry*; D015394:Molecular Structure; D009570:Nitriles / Q000737:chemistry*; D010084:Oxidation-Reduction; D013237:Stereoisomerism
chemical_list: D007659:Ketones; D009570:Nitriles; D000429:Alcohol Oxidoreductases