title: Association between the - 786T>C 1polymorphism in the promoter region of endothelial nitric oxide synthase (eNOS) and risk of coronary artery disease: a systematic review and meta-analysis.
abstract: BACKGROUND
  A variety of studies have evaluated the association between the -786T>C polymorphism in the promoter region of endothelial nitric oxide synthase (eNOS) and risk of coronary artery disease (CAD). However, the results remain conflicting. To better understand the role of eNOS -786T>C polymorphism in CAD risk, we conducted a comprehensive systematic review and meta-analysis.
  
  
  METHODS
  Case-control, cohort or cross-sectional studies evaluating the association between eNOS -786T>C polymorphism and CAD risk were searched in electronic databases of PubMed, ISI Web of Knowledge, Medline, Embase and Google Scholar Search (up to January 2013). Overall and subgroup analyses were performed. Odds ratio (OR) and 95% confidence interval (CI) were used to evaluate the association between eNOS -786T>C polymorphism and CAD risk. Statistical analysis was performed with Review Manager 5.0 and STATA12.0.
  
  
  RESULTS
  Twenty-four studies were analyzed between 6192 CAD cases and 9281 healthy controls. The combined results of overall analysis showed significant positive associations between CAD risk and eNOS -786T>C polymorphism in dominant model (OR=1.45, 95% CI=1.27-1.65), recessive model (OR=1.37, 95% CI=1.20-1.56), homozygote comparison (OR=1.64, 95% CI=1.31-2.04), heterozygote comparison (TC vs. TT, OR=1.39, 95% CI=1.23-1.57; CC vs. TC, OR=1.20, 95% CI=1.04-1.37) and allele comparison (OR=1.35, 95% CI=1.21-1.50). On subgroup analysis based on the ethnicity of population (Caucasians, Asians and others), significant differences were found in all genetic models for Caucasians, similar associations existed in Asians except heterozygote comparison (CC vs. TC). However, the associations were only found in dominant model, heterozygote comparison (TC vs. TT) and allele comparison for the populations named others.
  
  
  CONCLUSIONS
  Our investigations demonstrate the significant associations between eNOS -786C>T polymorphism and CAD risk, and this polymorphism might become an early marker for the risk evaluation of CAD.
authors.affiliation: Graduate School of Third Military Medical University, Chongqing 400038, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Zhouqin
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Department of Medical Genetics, College of Basic Medical Sciences, Third Military Medical University, Chongqing 400038, China.
authors.forename: Limeng
authors.initials: L
authors.lastname: Dai
authors.affiliation: Cardiovascular Research Institute and Department of Cardiology, Shenyang Northern Hospital, Shenyang 110840, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Cardiovascular Research Institute and Department of Cardiology, Shenyang Northern Hospital, Shenyang 110840, China.
authors.forename: Chenghui
authors.initials: C
authors.lastname: Yan
authors.affiliation: Cardiovascular Research Institute and Department of Cardiology, Shenyang Northern Hospital, Shenyang 110840, China. Electronic address: hanyaling01@sina.com.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Han
mesh_terms: D003324:Coronary Artery Disease / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D006801:Humans; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D044465:White People / Q000235:genetics
chemical_list: C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III
keywords: Coronary artery disease (CAD); Endothelial nitric oxide synthase (eNOS); Meta-analysis; Polymorphism