title: [Value of percentage of highly fluorescent lymphocytic cells for rapidly assessing septicemia in tumor patients].
abstract: OBJECTIVE
  To evaluate the value of percentage of highly fluorescent lymphocytic cells (HFLC%) for rapidly assessing septicemia in tumor patients.
  
  
  METHODS
  Blood samples were collected from 130 patients with tumors (60 septicemia patients and 70 non-septicemia patients) and 80 healthy controls. HFLC% was analyzed with Sysmex XE-5000, the level of C-reactive protein (CRP) measured with a commercially available turbidimetric immunoassay kit and the level of procalcitonin (PCT) determined with a semiquantitative chromatographic immunoassay kit. The diagnostic values of HFLC% and CRP in septicemia were evaluated with ROC analysis.
  
  
  RESULTS
  The values of HFLC% and CRP were significantly higher in the septicemia group than those in the non-septicemia and healthy groups (0.30% (0.10%-0.70%) vs 0.10% (0-0.20%), 0.10% (0-0.20%) ; 80.3 (28.5-129.5) vs 3.3 (1.4-41.4) , 1.4 (0.6-2.5) mg/L, all P < 0.01) . The ROC-AUCs for HFLC% and CRP for a diagnosis of septicemia were 0.72 (sensitivity 71.7%, specificity 58.7%) and 0.92 (sensitivity 96.7%, specificity 82.0%). Both of them could judge septicemia better. Additionally, HFLC% was correlated with the levels of PCT and CRP (r = 0.637, 0.241, both P < 0.01).
  
  
  CONCLUSIONS
  HFLC% may be used as a rapid and simple auxiliary indicator in the diagnosis of septicemia in patients with tumors. And it is conducive to make an early diagnosis of septicemia and avoid unnecessary use of antibiotics.
authors.affiliation: State Key Laboratory of Oncology in South China, Department of Clinical Laboratory, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
authors.forename: Yao-ling
authors.initials: YL
authors.lastname: Luo
authors.forename: Pei-guang
authors.initials: PG
authors.lastname: Xu
authors.forename: Song-guo
authors.initials: SG
authors.lastname: Peng
authors.forename: Jian-pei
authors.initials: JP
authors.lastname: Li
authors.forename: Miao-huan
authors.initials: MH
authors.lastname: Kuang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002097:C-Reactive Protein / Q000032:analysis; D002116:Calcitonin / Q000097:blood; D015740:Calcitonin Gene-Related Peptide; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003592:Cytophotometry / Q000379:methods*; D042241:Early Diagnosis; D005260:Female; D006801:Humans; D007223:Infant; D008214:Lymphocytes / Q000166:cytology; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000150:complications; D011498:Protein Precursors / Q000097:blood; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000097:blood* / Q000175:diagnosis* / Q000209:etiology; D055815:Young Adult
chemical_list: C000606589:CALCA protein, human; D011498:Protein Precursors; D002116:Calcitonin; D002097:C-Reactive Protein; D015740:Calcitonin Gene-Related Peptide