title: UXT is a novel regulatory factor of regulatory T cells associated with Foxp3.
abstract: Regulatory T (Treg) cells are a constitutively immunosuppressive subtype of T cells that contribute to the maintenance of immunological self-tolerance and immune homeostasis. However, the molecular mechanisms involved in the regulation of Treg cells remain unclear. In the present study, we identified ubiquitously expressed transcript (UXT) to be a novel regulator of human Treg-cell function. In cultured human Treg cells, UXT associates with Foxp3 in the nucleus by interacting with the proline-rich domain in the N-terminus of Foxp3. Knockdown of UXT expression in Treg cells results in a less-suppressive phenotype, demonstrating that UXT is an important regulator of the suppressive actions of Treg cells. Depletion of UXT affects the localization stability of Foxp3 protein in the nucleus and downregulates the expression of Foxp3-related genes. Overall, our results show that UXT is a cofactor of Foxp3 and an important player in Treg-cell function.
authors.affiliation: The State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Weina
authors.initials: W
authors.lastname: Li
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.forename: Changli
authors.initials: C
authors.lastname: Jiang
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Kuo
authors.initials: K
authors.lastname: Zhang
authors.forename: Yujin
authors.initials: Y
authors.lastname: Xu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Hao
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Xiaochang
authors.initials: X
authors.lastname: Xue
authors.forename: Xin
authors.initials: X
authors.lastname: Qin
authors.forename: Cun
authors.initials: C
authors.lastname: Zhang
authors.forename: Huixuan
authors.initials: H
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yingqi
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D018797:Cell Cycle Proteins; D002460:Cell Line; D002467:Cell Nucleus / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics / Q000276:immunology; D051858:Forkhead Transcription Factors / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D007108:Immune Tolerance / Q000235:genetics / Q000276:immunology; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D011392:Proline / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics / Q000276:immunology; D015003:Yeasts / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; C118920:UXT protein, human; D011392:Proline
keywords: Cofactor; Foxp3; Immune suppression; Regulatory T (Treg) cell; UXT