title: Peripheral blood stem cell collection in low-weight children: retrospective comparison of two apheresis devices.
abstract: BACKGROUND
  Apheresis is a major challenge in peripheral stem cell collection from low-weight children with cancer. Comparisons between the new apheresis device Optia (TerumoBCT) and the earlier COBE Spectra (CaridianBCT) have been performed in adults but not in low-weight children. The objective was to compare the performance of these two devices in small children.
  
  
  METHODS
  In this retrospective study, all patients were reviewed weighing less than 15 kg undergoing stem cell collection using the Optia device between April 2011 and April 2012. They were paired on weight in a 3:1 ratio with patients whose cells had been collected with the COBE Spectra since 2006.
  
  
  RESULTS
  Six patients were treated with the Optia and were matched with 18 patients treated with the Spectra. No side effects occurred. Collection efficiency (CE) was similar between the two groups (50% vs. 47%), but CD34 cell blood clearance was lower with the Optia (0.4 mL/min/kg vs. 0.6 mL/min/kg, p < 0.01). Platelet (PLT) loss and hemoglobin (Hb) loss were significantly reduced with the Optia (respectively, 32% vs. 54%, p < 0.01; and 1.4 g/dL vs. 2.9 g/dL, p < 0.01). Apheresis duration was increased with the Optia (159 min vs. 134 min, p < 0.05). The cell product harvested with the Optia had a lower volume and lower hematocrit, but similar white blood cell and PLT content.
  
  
  CONCLUSIONS
  Compared with the Spectra, the Optia allows similar CE with a reduced PLT and Hb loss but with a longer duration.
authors.affiliation: CHU Clermont-Ferrand, INSERM CIC 501, Clermont-Ferrand, France.
authors.forename: Bilade
authors.initials: B
authors.lastname: Cherqaoui
authors.forename: Nadège
authors.initials: N
authors.lastname: Rouel
authors.forename: Anne
authors.initials: A
authors.lastname: Auvrignon
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Defachelles
authors.forename: François
authors.initials: F
authors.lastname: Deméocq
authors.forename: Justyna
authors.initials: J
authors.lastname: Kanold
authors.forename: Etienne
authors.initials: E
authors.lastname: Merlin
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis; D001781:Blood Component Removal / Q000295:instrumentation*; D001835:Body Weight; D002648:Child; D002675:Child, Preschool; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007223:Infant; D008297:Male; D010976:Platelet Count; D012189:Retrospective Studies
chemical_list: D018952:Antigens, CD34; D006454:Hemoglobins