title: Disruption of LptA oligomerization and affinity of the LptA-LptC interaction.
abstract: The lipopolysaccharide (LPS)-rich outer membrane (OM) is a unique feature of Gram-negative bacteria, and LPS transport across the inner membrane (IM) and through the periplasm is essential to the biogenesis and maintenance of the OM. LPS is transported across the periplasm to the outer leaflet of the OM by the LPS transport (Lpt) system, which in Escherichia coli is comprised of seven recently identified proteins, including LptA, LptC, LptDE, and LptFGB2 . Structures of the periplasmic protein LptA and the soluble portion of the membrane-associated protein LptC have been solved and show these two proteins to be highly structurally homologous with unique folds. LptA has been shown to form concentration dependent oligomers that stack end-to-end. LptA and LptC have been shown to associate in vivo and are expected to form a similar protein-protein interface to that found in the LptA dimer. In these studies, we disrupted LptA oligomerization by introducing two point mutations that removed a lysine and glutamine side chain from the C-terminal β-strand of LptA. This loss of oligomerization was characterized using EPR spectroscopy techniques and the affinity of the interaction between the mutant LptA protein and WT LptC was determined using EPR spectroscopy (Kd = 15 µM) and isothermal titration calorimetry (Kd = 14 µM). Kd values were also measured by EPR spectroscopy for the interaction between LptC and WT LptA (4 µM) and for WT LptA oligomerization (29 µM). These data suggest that the affinity between LptA and LptC is stronger than the affinity for LptA oligomerization.
authors.affiliation: Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin, 53226.
authors.forename: Kathryn M
authors.initials: KM
authors.lastname: Schultz
authors.forename: Jimmy B
authors.initials: JB
authors.lastname: Feix
authors.forename: Candice S
authors.initials: CS
authors.lastname: Klug
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002151:Calorimetry; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002942:Circular Dichroism; D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D017354:Point Mutation; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D017385:Sequence Homology
chemical_list: D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C532155:LptA protein, E coli; C543318:LptC protein, E coli; D008565:Membrane Proteins; D011994:Recombinant Proteins
keywords: EPR spectroscopy; ITC; LPS binding protein; LptA; LptC; dissociation constants; protein oligomerization