title: Identification and characterization of a novel inhibitor of alginate overproduction in Pseudomonas aeruginosa.
abstract: In this study, we performed whole-genome complementation using a PAO1-derived cosmid library, coupled with in vitro transposon mutagenesis, to identify gene locus PA1494 as a novel inhibitor of alginate overproduction in P. aeruginosa strains possessing a wild-type mucA.
authors.affiliation: Department of Biochemistry and Microbiology, Joan C. Edwards School of Medicine at Marshall University, Huntington, WV, USA.
authors.forename: T Ryan
authors.initials: TR
authors.lastname: Withers
authors.forename: Yeshi
authors.initials: Y
authors.lastname: Yin
authors.forename: Hongwei D
authors.initials: HD
authors.lastname: Yu
mesh_terms: D000464:Alginates; D004251:DNA Transposable Elements; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D020723:Glucuronic Acid / Q000096:biosynthesis / Q000235:genetics; D006603:Hexuronic Acids; D016254:Mutagenesis, Insertional; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000464:Alginates; D004251:DNA Transposable Elements; D006603:Hexuronic Acids; D014157:Transcription Factors; D020723:Glucuronic Acid
keywords: PA1494; Pseudomonas aeruginosa; alginate; biofilms; inhibitor; muiA