title: Intravenous renal cell transplantation with SAA1-positive cells prevents the progression of chronic renal failure in rats with ischemic-diabetic nephropathy.
abstract: Diabetic nephropathy, the most common cause of progressive chronic renal failure and end-stage renal disease, has now reached global proportions. The only means to rescue diabetic patients on dialysis is renal transplantation, a very effective therapy but severely limited by the availability of donor kidneys. Hence, we tested the role of intravenous renal cell transplantation (IRCT) on obese/diabetic Zucker/SHHF F1 hybrid (ZS) female rats with severe ischemic and diabetic nephropathy. Renal ischemia was produced by bilateral renal clamping of the renal arteries at 10 wk of age, and IRCT with genetically modified normal ZS male tubular cells was given intravenously at 15 and 20 wk of age. Rats were euthanized at 34 wk of age. IRCT with cells expressing serum amyloid A had strong and long-lasting beneficial effects on renal function and structure, including tubules and glomeruli. However, donor cells were found engrafted only in renal tubules 14 wk after the second infusion. The results indicate that IRCT with serum amyloid A-positive cells is effective in preventing the progression of chronic kidney disease in rats with diabetic and ischemic nephropathy.
authors.affiliation: Veterans Affairs Medical Center, N111, 1481 W. 10th St., Indianapolis, IN 46202. jhdoming@iupui.edu.
authors.forename: Katherine J
authors.initials: KJ
authors.lastname: Kelly
authors.forename: Jizhong
authors.initials: J
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Han
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Wang
authors.forename: Shaobo
authors.initials: S
authors.lastname: Zhang
authors.forename: Jesus H
authors.initials: JH
authors.lastname: Dominguez
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000150:complications; D003928:Diabetic Nephropathies / Q000150:complications*; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D007511:Ischemia / Q000150:complications; D007668:Kidney / Q000098:blood supply / Q000166:cytology* / Q000378:metabolism*; D007676:Kidney Failure, Chronic / Q000209:etiology* / Q000517:prevention & control*; D008297:Male; D009765:Obesity / Q000150:complications; D051381:Rats; D011924:Rats, Zucker; D000685:Serum Amyloid A Protein / Q000378:metabolism*; D019737:Transplants; D016896:Treatment Outcome
chemical_list: C489995:Saa2 protein, mouse; D000685:Serum Amyloid A Protein
keywords: chronic renal failure; diabetic nephropathies; organ regeneration; serum amyloid A