title: Selection of a mimotope peptide of S-adenosyl-L-homocysteine and its application in immunoassays.
abstract: A competitive immunoassay for S-adenosyl-L-homocysteine (SAH) has been used in the clinical test for homocysteine via an enzymatic conversion reaction. Since S-adenosyl-l-homocysteine is a relatively unstable compound, we have used peptide library phage display to select a new mimotope peptide that interacts with the anti-SAH antibody. By immobilizing the synthetic peptide on solid phase as a competitive surrogate for SAH, we demonstrate its utility in a competitive ELISA assay. The linear range of the assay for SAH was 0.4-6.4 µM, in good correlation to the conventional assay using an SAH-conjugated plate. Our results show that the mimotope peptide has potential to substitute for SAH in immunoassays.
authors.affiliation: Health Research Institute, AIST, 1-8-31 Midorigaoka, Ikeda 563-8577, Osaka, Japan. tzertzinis@neb.com.
authors.forename: Chun
authors.initials: C
authors.lastname: Wu
authors.forename: George
authors.initials: G
authors.lastname: Tzertzinis
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D018716:Molecular Mimicry; D009842:Oligopeptides / Q000737:chemistry* / Q000276:immunology; D019151:Peptide Library; D011485:Protein Binding; D012435:S-Adenosylhomocysteine / Q000737:chemistry* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D009842:Oligopeptides; D019151:Peptide Library; D012435:S-Adenosylhomocysteine