title: Induction of adhesion molecule expression in co-culture of human bronchial epithelial cells and neutrophils suppressed by puerarin via down-regulating p38 mitogen-activated protein kinase and nuclear factor κB pathways.
abstract: OBJECTIVE
  In this study, we aimed to investigate the expressions of adhesion molecules on human bronchial epithelial cells and neutrophils in co-culture system, assess the effects of puerarin on suppressing these adhesion molecules expressions, and explore the roles of two crucial signal-transduction elements p38 mitogen-activated protein kinase (p38 MAPK) and nuclear factor kappa B (NF-κB) in modulating adhesion molecules expressions.
  
  
  METHODS
  Neutrophils and BEAS-2B cells (one human bronchial epithelial cell line) were co-cultured, and adhesion molecules expressions on cell surface were detected using flow cytometry. The mRNA levels of adhesion molecules were assessed by real-time quantitative polymerase chain reaction (real-time qPCR). Phosphorylated p38 MAPK and inhibitor κB were analyzed by Western blot.
  
  
  RESULTS
  In co-culture system, adhesion molecules expressions on BEAS-2B cells and neutrophils were enhanced significantly (P<0.05). Correspondingly, the mRNA levels of adhesion molecules were also increased greatly. Moreover, the pretreatment of peurarin obviously suppressed adhesion molecules expressions on cell surface. Furthermore, phosphorylated p38 MAPK and inhibitor κB in BEAS-2B cells and neutrophils were elevated in co-culture system, but decreased significantly after upon the treatment of peurarin (P<0.05).
  
  
  CONCLUSIONS
  Coculture boosted the interactions between human bronchial epithelial cells and neutrophils mimicking airway inflflammation, whereas peurarin decreased the expression of adhesion molecules on cell surface by suppressing the activities of p38 MAPK and NF-κB pathways, and exhibiting its anti-inflflammation activity.
authors.forename: Ye
authors.initials: Y
authors.lastname: Liu
authors.forename: Ling-li
authors.initials: LL
authors.lastname: Shao
authors.forename: Wei
authors.initials: W
authors.lastname: Pang
authors.forename: Xiao-mei
authors.initials: XM
authors.lastname: Lan
authors.forename: Jian-xin
authors.initials: JX
authors.lastname: Lu
authors.forename: Yu-long
authors.initials: YL
authors.lastname: Cong
authors.forename: Cheng-bin
authors.initials: CB
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D001980:Bronchi / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D002417:Cattle; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002460:Cell Line; D018920:Coculture Techniques; D017931:DNA Primers; D015536:Down-Regulation / Q000187:drug effects; D004847:Epithelial Cells / Q000201:enzymology / Q000378:metabolism; D007529:Isoflavones / Q000494:pharmacology*; D016328:NF-kappa B / Q000378:metabolism*; D009504:Neutrophils / Q000201:enzymology / Q000378:metabolism; D010766:Phosphorylation; D060888:Real-Time Polymerase Chain Reaction; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D015815:Cell Adhesion Molecules; D017931:DNA Primers; D007529:Isoflavones; D016328:NF-kappa B; D048051:p38 Mitogen-Activated Protein Kinases; C033607:puerarin