title: Chronic depression of hypothalamic paraventricular neuronal activity produces sustained hypotension in hypertensive rats.
abstract: Changes in the sympathetic nervous system are responsible for the initiation, development and maintenance of hypertension. An important central sympathoexcitatory region is the paraventricular nucleus (PVN) of the hypothalamus, which may become more active in hypertensive conditions, as shown in acute studies previously. Our objective was to depress PVN neuronal activity chronically by the overexpression of an inwardly rectifying potassium channel (hKir2.1), while evaluating the consequences on blood pressure (BP) and its reflex regulation. In spontaneously hypertensive rats (SHRs) and Wistar rats (WKY) lentiviral vectors (LVV-hKir2.1; LV-TREtight-Kir-cIRES-GFP5 4 × 10(9) IU and LV-Syn-Eff-G4BS-Syn-Tetoff 6.2 × 10(9) IU in a ratio 1:4) were stereotaxically microinjected bilaterally into the PVN. Sham-treated SHRs and WKY received bilateral PVN microinjections of LVV-eGFP (LV-Syn-Eff-G4BS-Syn-Tetoff 6.2 × 10(9) IU and LV-TREtight-GFP 5.7 × 10(9) IU in a ratio 1:4). Blood pressure was monitored continuously by radio-telemetry and evaluated over 75 days. Baroreflex gain was evaluated using phenylephrine (25 μg ml(-1), i.v.), whereas lobeline (25 μg ml(-1), i.v.) was used to stimulate peripheral chemoreceptors. In SHRs but not normotensive WKY rats, LVV-hKir2.1 expression in the PVN produced time-dependent and significant decreases in systolic (from 158 ± 3 to 132 ± 6 mmHg; P < 0.05) and diastolic BP (from 135 ± 4 to 113 ± 5 mmHg; P < 0.05). The systolic BP low-frequency band was reduced (from 0.79 ± 0.13 to 0.42 ± 0.09 mmHg(2); P < 0.05), suggesting reduced sympathetic vasomotor tone. Baroreflex gain was increased and peripheral chemoreflex depressed after PVN microinjection of LVV-hKir2.1. We conclude that the PVN plays a major role in long-term control of BP and sympathetic nervous system activity in SHRs. This is associated with reductions in both peripheral chemosensitivity and respiratory-induced sympathetic modulation and an improvement in baroreflex sensitivity. Our results support the PVN as a powerful site to control BP in neurogenic hypertension.
authors.affiliation: I. Rocha: Instituto de Fisiologia, Faculdade de Medicina de Lisboa, Av Prof Egas Moniz, 1649-028 Lisbon, Portugal. isabelrocha@fm.ul.pt.
authors.forename: Vera
authors.initials: V
authors.lastname: Geraldes
authors.forename: Nataniel
authors.initials: N
authors.lastname: Gonçalves-Rosa
authors.forename: Beihui
authors.initials: B
authors.lastname: Liu
authors.forename: Julian F R
authors.initials: JF
authors.lastname: Paton
authors.forename: Isabel
authors.initials: I
authors.lastname: Rocha
mesh_terms: D000818:Animals; D017704:Baroreflex / Q000502:physiology; D001794:Blood Pressure / Q000502:physiology; D002628:Chemoreceptor Cells / Q000378:metabolism / Q000502:physiology; D006339:Heart Rate / Q000502:physiology; D006973:Hypertension / Q000378:metabolism / Q000503:physiopathology*; D007031:Hypothalamus / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D008845:Microinjections / Q000379:methods; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism / Q000503:physiopathology*; D024661:Potassium Channels, Inwardly Rectifying / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR / Q000378:metabolism / Q000502:physiology*; D011921:Rats, Inbred WKY; D017208:Rats, Wistar; D012119:Respiration; D013564:Sympathetic Nervous System / Q000378:metabolism / Q000503:physiopathology; D014661:Vasoconstriction / Q000502:physiology; D014666:Vasomotor System / Q000378:metabolism / Q000503:physiopathology*
chemical_list: C489457:KCNJ2 protein, human; D024661:Potassium Channels, Inwardly Rectifying