title: Succinic acid production from corn stalk hydrolysate in an E. coli mutant generated by atmospheric and room-temperature plasmas and metabolic evolution strategies.
abstract: AFP111 is a spontaneous mutant of Escherichia coli with mutations in the glucose-specific phosphotransferase system, pyruvate formate lyase system, and fermentative lactate dehydrogenase system, created to reduce byproduct formation and increase succinic acid accumulation. In AFP111, conversion of xylose to succinic acid only generates 1.67 ATP per xylose, but requires 2.67 ATP for xylose metabolism. Therefore, the ATP produced is not adequate to accomplish the conversion of xylose to succinic acid in chemically defined medium. An E. coli mutant was obtained by atmospheric and room-temperature plasmas and metabolic evolution strategies, which had the ability to use xylose and improve the capacity of cell growth. The concentration of ATP in the mutant was 1.33-fold higher than that in AFP111 during xylose fermentation. In addition, under anaerobic fermentation with almost 80 % xylose from corn stalk hydrolysate, a succinic acid concentration of 21.1 g l(-1) was obtained, with a corresponding yield of 76 %.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211816, People's Republic of China, bioengine@njut.edu.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Qing
authors.initials: Q
authors.lastname: Wan
authors.forename: Rongming
authors.initials: R
authors.lastname: Liu
authors.forename: Liya
authors.initials: L
authors.lastname: Liang
authors.forename: Xu
authors.initials: X
authors.lastname: Chen
authors.forename: Mingke
authors.initials: M
authors.lastname: Wu
authors.forename: Hanwen
authors.initials: H
authors.lastname: Zhang
authors.forename: Kequan
authors.initials: K
authors.lastname: Chen
authors.forename: Jiangfeng
authors.initials: J
authors.lastname: Ma
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
authors.forename: Pingkai
authors.initials: P
authors.lastname: Ouyang
mesh_terms: D000123:Acetyltransferases / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D060847:Metabolic Engineering; D009154:Mutation; D019802:Succinic Acid / Q000378:metabolism*; D013696:Temperature; D014994:Xylose / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism*
chemical_list: D014994:Xylose; D019802:Succinic Acid; D000123:Acetyltransferases; C022560:formate C-acetyltransferase; D005947:Glucose