title: [Effects of PEG and NaCl stress on the contents of indigotin and indirubin in Isatis indigotica tetraploids].
abstract: OBJECTIVE
  To provide a reference evidence for choosing salt-resistent and drought-resistent varieties of Isatis indigotica Fort..
  
  
  METHODS
  The tissue culture seedlings of different Isatis indigotica tetraploids were used as plant materials. The diploid was used as CK, which were cultured for 30 days. 2 g/L NaCl and 15% PEG-6000 were used as stress treatment respectively for 12 hours, then poured out the solution, the materials were continuously cultured for 7 days. The contents of indirubin and indigotin were determined by HPLC.
  
  
  RESULTS
  The contents of indigotin and indirubin of DB3, DB5 and DB12 with PEG treatment and DB2, DB3 and DB12 with NaCl treatment were increased.
  
  
  CONCLUSIONS
  DB3 and DB12 can be further studied as reproduction materials.
authors.affiliation: Department of Life Sciences, Tangshan Normal College, Tangshan 063000, China. keshaoying@sina.com
authors.forename: Shao-Ying
authors.initials: SY
authors.lastname: Ke
authors.forename: Yan-Zhi
authors.initials: YZ
authors.lastname: Ma
mesh_terms: D002851:Chromatography, High Pressure Liquid; D007203:Indigo Carmine / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry; D031219:Isatis / Q000737:chemistry / Q000187:drug effects*; D018517:Plant Roots / Q000737:chemistry / Q000187:drug effects; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology*; D011786:Quality Control; D036226:Seedlings / Q000737:chemistry / Q000187:drug effects; D012965:Sodium Chloride / Q000737:chemistry / Q000494:pharmacology*; D013312:Stress, Physiological; D057891:Tetraploidy; D014867:Water / Q000737:chemistry
chemical_list: D007211:Indoles; D014867:Water; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D007203:Indigo Carmine; C027185:indirubin