title: Altered expression of pannexin proteins in patients with temporal lobe epilepsy.
abstract: The aim of the present study was to investigate the expression of the pannexin (Panx) proteins, Panx1 and Panx2, in the temporal lobe tissue of patients with temporal lobe epilepsy (TLE). Immunohistochemistry and western blotting methods were used to localize and quantify Panx1 and Panx2 in the surgically removed brain tissue of patients with TLE (n=37). The results were then compared with non-epileptogenic controls (n=9). Panx1 and Panx2 expression was detected in the temporal lobe cortex of patients with TLE and in the control tissues. Panx1 and Panx2 proteins were expressed in all layers of the epileptic cortex, but predominantly in layers II and III of the cortex in the control group. Panx1 protein expression was significantly higher in the temporal lobe cortex of the patients with TLE than in the controls (P<0.05; t-test); however, no significant differences were identified in the Panx2 expression levels between the patients and the controls (P>0.05; t-test). The expression of the two Panx proteins in the tissue layers of the epileptic cortex varied in the patients and controls. The results indicate that Panx channels may be involved in the pathogenesis of TLE.
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Jiang
authors.forename: Hongyu
authors.initials: H
authors.lastname: Long
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
authors.forename: Lili
authors.initials: L
authors.lastname: Long
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Pinting
authors.initials: P
authors.lastname: Zhou
authors.forename: Chunyun
authors.initials: C
authors.lastname: Yuan
authors.forename: Bo
authors.initials: B
authors.lastname: Xiao
mesh_terms: D000293:Adolescent; D000328:Adult; D015153:Blotting, Western; D016022:Case-Control Studies; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D017630:Connexins / Q000378:metabolism*; D004833:Epilepsy, Temporal Lobe / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D055815:Young Adult
chemical_list: D017630:Connexins; D009419:Nerve Tissue Proteins; C492764:PANX1 protein, human; C492765:PANX2 protein, human