title: A novel chemiluminescence assay of mitoxantrone based on diperiodatocuprate(III) oxidation.
abstract: A novel and strong chemiluminescence (CL) of luminol with diperiodatocuprate (K5[Cu(HIO6)2]) was observed in alkaline medium. After the addition of mitoxantrone (MTX) into this system, the CL intensity could be greatly inhibited by MTX. Based on the phenomenon, a sensitive CL method was established for analysis of MTX combining with flow injection technology. Under optimum experimental conditions, the CL intensity was linearly related to the logarithm concentration of MTX from 5.0×10(-9)-1.0×10(-7) g/ml with the detection limit of 1.1×10(-9) g/ml (S/N=3). The relative standard deviation was 1.2% for 5.0×10(-8) g/ml of MTX. The proposed method was successfully applied for determination of MTX in pharmaceutical preparations and biological fluids. The possible CL reaction mechanism was also discussed briefly.
authors.affiliation: School of Pharmaceutical Science, Zhengzhou University, Zhengzhou, China.
authors.forename: Hanchun
authors.initials: H
authors.lastname: Yao
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Zeng
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zeng
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000097:blood*; D001681:Biological Assay; D003300:Copper / Q000737:chemistry*; D017022:Flow Injection Analysis; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D051379:Mice; D008942:Mitoxantrone / Q000097:blood*; D008958:Models, Molecular; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000032:analysis*
chemical_list: D000970:Antineoplastic Agents; D004364:Pharmaceutical Preparations; D010504:Periodic Acid; D008165:Luminol; D003300:Copper; D008942:Mitoxantrone
keywords: Biological fluids; Chemiluminescence; Diperiodatocuprate; Mitoxantrone