title: Surface reconstruction and hemocompatibility improvement of a phosphorylcholine end-capped poly(butylene succinate) coating.
abstract: Control over cell-material surface interactions is the key to many new and improved biomedical devices. In this study, we present a simple yet effective surface modification method that allows for the surface reconstruction and formation of cell outer membrane mimetic structure on coatings that have significantly increased hemocompatibility. To achieve this, a phosphorylcholine end-capped poly(butylene succinate) (PBS-PC) was synthesized and dip-coated on coverslips. The surface structure of the amphiphilic PBS-PC film was reconstructed by heating in a vacuum oven to obtain the less hydrophilic surface and by immersing in hot water to obtain the more hydrophilic surface. Significant changes in the surface element concentration were observed by X-ray photoelectron spectroscopy analysis and changes in surface wettability were measured by sensitive dynamic contact angle technique. Scanning electron microscope images showed different morphologies of the reconstructed surfaces. Interestingly, the reconstruction between the less hydrophilic and more hydrophilic surfaces is reversible. More importantly, both the reconstructed surfaces are stable in room condition for more than 6 months, and both the surfaces show significant improvement in hemocompatibility as revealed by protein adsorption and platelet adhesion measurements. This reversible surface reconstruction strategy and the interesting results may be significant for fabricating stable and hemocompatible surfaces on differently shaped biomedical devices.
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Northwest University, Xi'an, Shaanxi, China; College of Chemistry and Materials Science, Northwest University, Xi'an, Shaanxi, China.
authors.forename: Ni
authors.initials: N
authors.lastname: Hao
authors.forename: Yan-Bing
authors.initials: YB
authors.lastname: Wang
authors.forename: Shi-Ping
authors.initials: SP
authors.lastname: Zhang
authors.forename: Su-Qing
authors.initials: SQ
authors.lastname: Shi
authors.forename: Kenichi
authors.initials: K
authors.lastname: Nakashima
authors.forename: Yong-Kuan
authors.initials: YK
authors.lastname: Gong
mesh_terms: D001792:Blood Platelets / Q000166:cytology; D002072:Butylene Glycols / Q000737:chemistry* / Q000378:metabolism; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D010767:Phosphorylcholine / Q000737:chemistry* / Q000378:metabolism; D056951:Photoelectron Spectroscopy; D010973:Platelet Adhesiveness; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties
chemical_list: D002072:Butylene Glycols; D020099:Coated Materials, Biocompatible; D011108:Polymers; C089797:bionole; D010767:Phosphorylcholine
keywords: contact angle; hemocompatibility; phosphorylcholine; poly(butylene succinate); surface reconstruction