title: E-assay concept: detection of bisphenol A with a label-free electrochemical competitive immunoassay.
abstract: A label-free electrochemical immunosensor was developed by electropolymerization of N-(3-(4-(2-(4-hydroxyphenyl)propan-2-yl)phenoxy)propyl) 3-(5-hydroxy-1,4-dihydro-1,4-dioxonaphthalen-2(3)-yl)propionamide (JugBPA). By combination with an antibody directed to bisphenol A (αBPA), this conducting polymer-based biosensor can detect BPA directly with a limit of detection of 2pgmL(-1). Square wave voltammetry shows that the polymer film presents a current decrease upon anti-BPA binding and an opposite current increase upon BPA addition in solution. This electrochemical immunosensor (E-assay) also shows high selectivity towards closely related compounds (bisphenol A dimethacrylate, and dibutyl phthalate). The E-assay concept described here could be a promising tool for simple, low-cost and reagentless on-site environmental monitoring.
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Reisberg
authors.forename: N
authors.initials: N
authors.lastname: Serradji
authors.forename: G
authors.initials: G
authors.lastname: Anquetin
authors.forename: M-C
authors.initials: MC
authors.lastname: Pham
authors.forename: W
authors.initials: W
authors.lastname: Wu
authors.forename: C-Z
authors.initials: CZ
authors.lastname: Dong
authors.forename: B
authors.initials: B
authors.lastname: Piro
mesh_terms: D000577:Amides / Q000737:chemistry*; D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D001559:Benzhydryl Compounds / Q000276:immunology / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000379:methods*; D004784:Environmental Monitoring; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay; D057230:Limit of Detection; D009285:Naphthoquinones / Q000737:chemistry*; D010636:Phenols / Q000276:immunology / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry; D000098826:Bisphenol A Compounds
chemical_list: D000577:Amides; D000906:Antibodies; D001559:Benzhydryl Compounds; D006046:Gold; D009285:Naphthoquinones; D010636:Phenols; D011108:Polymers; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A detection; Competitive immunoassay; Electrochemical immunosensor; Label-free detection