title: Effect of ERBB2 expression on invasiveness of glioma TJ905 cells.
abstract: OBJECTIVE
  To investigate the influence and possible mechanism of ERBB2 expression on the invasiveness of glioma cells.
  
  
  METHODS
  Glioma TJ905 cells were separated and cultured. ERBB2 shRNA and overexpressing vectors were constructed, which were then transfected. The ERBB2 expression was up-regulated or down-regulated. Changes of invasiveness of TJ905 cells were detected by Transwell assay, and the expressions of matrix metalloprotease (MMP)-2 and MMP-9 were measured by Western blot.
  
  
  RESULTS
  ERBB2 shRNA transfection vector could effectively inhibit expression of ERBB2; while ERBB2 overexpressing vector transfection could significantly improve the expression of ERBB2 in TJ905 cells. Transwell assay showed that when ERBB2 expression was down-regulated, the invasiveness of TJ905 cells was notably decreased; when ERBB2 expression was up-regulated, the invasiveness of TJ905 cells was markedly increased. Meanwhile, Western blot indicated that down-regulating ERBB2 inhibited the expression of MMP-2 and MMP-9, while up-regulating ERBB2 enhanced their expressions.
  
  
  CONCLUSIONS
  ERBB2 expression is closely related to the invasiveness of glioma TJ905 cells.
authors.affiliation: Department of Neurosurgery, First Hospital of Xi'an, Jiaotong University, Xi'an 710061, China.
authors.forename: Gao-Feng
authors.initials: GF
authors.lastname: Xu
authors.forename: Wan-Fu
authors.initials: WF
authors.lastname: Xie
mesh_terms: D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D005910:Glioma / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics*; D034741:RNA, Small Interfering / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D034741:RNA, Small Interfering; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human
keywords: Glioma; Invasiveness; Stem cells