title: Immunoexpression of proliferating cell nuclear antigen (PCNA) in spleen of splenectomized rats with preservation of inferior pole, submitted to hyperbaric oxygenation.
abstract: OBJECTIVE
  To analyze PCNA immunoexpression on the inferior pole of the spleen of splenectomized rats submitted to hyperbaric oxygenation (HBO).
  
  
  METHODS
  Were analyzed fragments of the inferior pole of the spleen of 20 male Wistar rats submitted to splenectomy with preservation of the inferior pole. The rats were divided in two groups: group A (n=10) without HBO and group B (n=10) submitted to HBO at 2, 5 atmospheres per 120 minutes, twice a day for three days and once a day for seven days. The groups were then subdivided in four subgroups: A15 (n=5), with euthanasia on the 15th day; A45 (n=5), with euthanasia on the 45th day; B15 (n=5) with euthanasia on the 15th day and B45 with euthanasia on the 45th day. Respectively on these days, fragments of the inferior pole of the spleen of all animals were collected and analyzed with the immunohistochemistry technique in order to evaluate PCNA expression.
  
  
  RESULTS
  There was an expressive increase in PCNA immunoreactivity in the group B. The 45 day postoperative period resulted in a higher level of positivity than the 15 day postoperative period (p<0.01).
  
  
  CONCLUSIONS
  The quantitative analysis of proliferating cell nuclear antigen positive suggests that hyperbaric oxygenation increases cellular proliferation, contributing to splenic regeneration.
authors.forename: Marcela Souza Lima
authors.initials: MS
authors.lastname: Paulo
authors.forename: Filipy Taifson Barbosa dos
authors.initials: FT
authors.lastname: Santos
authors.forename: Polyanna Gonçalves
authors.initials: PG
authors.lastname: Rocha
authors.forename: Mirella de Barros
authors.initials: Mde B
authors.lastname: Silva
authors.forename: Luiz Cálice
authors.initials: LC
authors.lastname: Cintra
authors.forename: Luciene Lage da
authors.initials: LL
authors.lastname: Motta
authors.forename: Flávia Imbroisi Valle
authors.initials: FI
authors.lastname: Errera
authors.forename: Danilo Nagib Salomão
authors.initials: DN
authors.lastname: Paulo
authors.forename: Tarcizo Afonso
authors.initials: TA
authors.lastname: Nunes
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D004195:Disease Models, Animal; D006931:Hyperbaric Oxygenation / Q000379:methods*; D007150:Immunohistochemistry; D008297:Male; D011184:Postoperative Period; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D011897:Random Allocation; D017208:Rats, Wistar; D013154:Spleen / Q000276:immunology* / Q000601:surgery; D013156:Splenectomy / Q000379:methods*
chemical_list: D018809:Proliferating Cell Nuclear Antigen