title: Determination of stilbenes and resorcylic acid lactones in bovine, porcine and poultry muscle tissue by liquid chromatography-negative ion electrospray mass spectrometry and QuEChERS for sample preparation.
abstract: Liquid chromatography tandem mass spectrometry method was developed and validated to confirm of resorcylic acid lactones: zeranol, taleranol, zearalanone, zearalenone, α and β-zearalenol and stilbenes in muscle tissue. The compounds were analyzed by LC-MS/MS QTrap 5500 apparatus in negative ionization mode. Chromatographic separation on Poroshell 120-EC C18 (150mm×2.1mm, 2.7μm) column was achieved at 45°C using isocratic elution of mobile phase - methanol/water (65:35, v/v). For the treatment of tissue samples prior to analysis, QuEChERS method was applied based on the extraction of analytes from muscle samples with ethyl acetate, separation of the aqueous and organic phases with application of magnesium sulphate and sodium acetate, the purification of the extract obtained by dispersive SPE with the use of sorbent C18, PSA and magnesium sulphate. The method was validated in accordance with the Commission Decision 2002/657/EC. Good recoveries were obtained (from 83% to 115%) as well as acceptable within-lab reproducibility (<22%). The values of the decision limit CCα and the detection capability CCβ for individual compounds are found to be below the recommended concentration set at 1μgkg(-1) and not exceed 0.23μgkg(-1) and 0.39μgkg(-1), respectively. The elaborated method meets the criteria for confirmatory methods and is used in the official control of hormones.
authors.affiliation: Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland. Electronic address: bwozniak@piwet.pulawy.pl.
authors.forename: Barbara
authors.initials: B
authors.lastname: Wozniak
authors.forename: Iwona Matraszek
authors.initials: IM
authors.lastname: Zuchowska
authors.forename: Jan
authors.initials: J
authors.lastname: Zmudzki
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D057230:Limit of Detection; D016014:Linear Models; D008460:Meat / Q000592:standards; D009132:Muscles / Q000737:chemistry*; D015203:Reproducibility of Results; D012118:Resorcinols / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013267:Stilbenes / Q000032:analysis*; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D012118:Resorcinols; D013267:Stilbenes
keywords: Liquid chromatography; Mass spectrometry; QuEChERS; RALs; Stilbenes