title: Genomic and expression analysis of a solute carrier protein (CcSLC25a5) gene from Cyprinus carpio Linnaeus.
abstract: Using the Genefishing method, we identified seven potential regulatory genes involved in the process of scale morphogenesis in fishes. We further characterized a novel solute carrier protein gene (CcSLC), from the common carp which is differentially expressed in mirror carp and Jianli. The ORF encodes a peptide of 298 amino acids with a molecular mass of 31.5 kDa and a theoretical isoelectric point of 7.49. ScanProsite analysis indicated that it is a putative solute carrier protein that contains a substrate binding site. CcSLC was detected in carp embryos by in situ hybridization in the 70%-epiboly, 6-somite, and 14-somite embryonic stages. Gene expression stopped at the long pec stage. However, CcSLC25a5 was re-expressed during the initiation of scale formation in the regions that were scale covered. These findings provide novel insights into the features of early carp embryo and scale development.
authors.affiliation: College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023 China.
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.forename: Anda
authors.initials: A
authors.lastname: Cheng
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Wang
authors.forename: Baoyong
authors.initials: B
authors.lastname: Zhang
keywords: Cyprinus carpio Linnaeus; Embryo development; Scale development; Scale initiation; Solute carrier protein