title: Desulfatiferula berrensis sp. nov., a n-alkene-degrading sulfate-reducing bacterium isolated from estuarine sediments.
abstract: A novel sulfate-reducing bacterium designated strain BE2801(T) was isolated from oil-polluted estuarine sediments (Berre Lagoon, France). Cells were Gram-stain-negative, motile, slightly curved or vibrioid rods. Optimal growth of strain BE2801(T) occurred at 30-32 °C, 0.5-1.5% NaCl (w/v) and pH 7.2-7.4. Strain BE2801(T) grew with C4 to C20 fatty acids or C12 to C20 n-alkenes as electron donors. Acetate and carbon dioxide were the oxidation products. The major cellular fatty acids were C16 : 0, C(16 : 1)ω7c and C(18 : 1)ω7. The DNA G+C content was 50.2 mol%. 16S rRNA and dsrAB gene sequence analysis indicated that strain BE2801(T) was a member of the family Desulfobacteraceae within the class Deltaproteobacteria. DNA-DNA hybridization with the most closely related taxon demonstrated 14.8 % relatedness. Based on phenotypic and phylogenetic evidence, strain BE2801(T) ( = DSM 25524(T) = JCM 18157(T)) is proposed to be a representative of a novel species of the genus Desulfatiferula, for which the name Desulfatiferula berrensis sp. nov. is suggested.
authors.affiliation: Equipe Environnement et Microbiologie, IPREM UMR 5254, IBEAS Université de Pau et des Pays de l'Adour, BP1155, 64013 Pau cedex, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Hakil
authors.affiliation: Université de Toulon, Université du Sud Toulon-Var, CNRS/INSU, IRD, Mediterranean Institute of Oceanography (MIO), UM 110, 83957 La Garde, France.|Aix-Marseille Université, CNRS/INSU, IRD, Mediterranean Institute of Oceanography (MIO), UM 110, 13288 Marseille, France.
authors.forename: Oulfat
authors.initials: O
authors.lastname: Amin-Ali
authors.affiliation: Université de Toulon, Université du Sud Toulon-Var, CNRS/INSU, IRD, Mediterranean Institute of Oceanography (MIO), UM 110, 83957 La Garde, France.|Aix-Marseille Université, CNRS/INSU, IRD, Mediterranean Institute of Oceanography (MIO), UM 110, 13288 Marseille, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Hirschler-Réa
authors.affiliation: Laboratoire de Géologie de Lyon: Terre, Planètes, Environnement, UMR CNRS 5276, Université Claude Bernard Lyon 1, Campus de la Doua, 69622 Villeurbanne, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Mollex
authors.affiliation: Laboratoire de Géologie de Lyon: Terre, Planètes, Environnement, UMR CNRS 5276, Université Claude Bernard Lyon 1, Campus de la Doua, 69622 Villeurbanne, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Grossi
authors.affiliation: Equipe Environnement et Microbiologie, IPREM UMR 5254, IBEAS Université de Pau et des Pays de l'Adour, BP1155, 64013 Pau cedex, France.
authors.forename: Robert
authors.initials: R
authors.lastname: Duran
authors.affiliation: Aix-Marseille Université, IMBE, UMR 7263, UR-IRD 237, 13397 Marseille cedex 20, France.
authors.forename: Robert
authors.initials: R
authors.lastname: Matheron
authors.affiliation: Equipe Environnement et Microbiologie, IPREM UMR 5254, IBEAS Université de Pau et des Pays de l'Adour, BP1155, 64013 Pau cedex, France.
authors.forename: Cristiana
authors.initials: C
authors.lastname: Cravo-Laureau
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D020564:Deltaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D063366:Estuaries; D005227:Fatty Acids / Q000737:chemistry; D005602:France; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D059392:Petroleum Pollution; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016999:Sulfur-Reducing Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D062065:Wastewater
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D062065:Wastewater