title: An in vitro study to determine the minimal bactericidal concentration of sodium hypochlorite (bleach) required to inhibit meticillin-resistant Staphylococcus pseudintermedius strains isolated from canine skin.
abstract: BACKGROUND
  Meticillin-resistant Staphylococcus pseudintermedius (MRSP) is becoming a more common pathogen in animals treated in veterinary hospitals, raising concerns for transmission and possible contamination of the hospital itself. The identification of effective agents for the decontamination of veterinary patients and hospitals is crucial. Sodium hypochlorite (6.15%) or 'bleach' is an inexpensive and frequently used decontamination agent in human and veterinary hospitals and an adjunctive treatment for human patients. There are no published data on the minimal bactericidal concentration (MBC) of sodium hypochlorite (6.15%) for MRSP.
  
  
  OBJECTIVE
  The objective of this study was to determine the MBC of sodium hypochlorite (6.15%) against MRSP strains isolated from canine skin.
  
  
  METHODS
  In this in vitro study, 12 canine skin isolates were obtained from the Antech Diagnostics microbiology department. Twofold serial dilutions of sodium hypochlorite (6.15%) were allowed to react with each of the MRSP strains for 15 min, followed by overnight incubation on agar plates. Colonies on each plate were counted. This process was repeated in triplicate.
  
  
  RESULTS
  The overall MBC for the canine skin-isolated MRSP strains was 1:32, but most strains had an MBC between 1:64 and 1:128 dilution.
  
  
  CONCLUSIONS
  This study shows that sodium hypochlorite (6.15%) is an effective agent for decontamination at easily achievable concentrations.
authors.affiliation: Animal Allergy and Dermatology - Dermatology, 1124 Lynnhaven Parkway, Virginia Beach, VA, 23453, USA.
authors.forename: Marlene
authors.initials: M
authors.lastname: Pariser
authors.forename: Sharon
authors.initials: S
authors.lastname: Gard
authors.forename: Dunbar
authors.initials: D
authors.lastname: Gram
authors.forename: Lynn
authors.initials: L
authors.lastname: Schmeitzel
mesh_terms: D000818:Animals; D004202:Disinfectants / Q000494:pharmacology*; D004285:Dogs / Q000382:microbiology*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D012867:Skin / Q000382:microbiology*; D012973:Sodium Hypochlorite / Q000494:pharmacology*
chemical_list: D004202:Disinfectants; D012973:Sodium Hypochlorite