title: Identification and characterization of a cold-active phthalate esters hydrolase by screening a metagenomic library derived from biofilms of a wastewater treatment plant.
abstract: A cold-active phthalate esters hydrolase gene (designated dphB) was identified through functional screening of a metagenomic library derived from biofilms of a wastewater treatment plant. The enzyme specifically catalyzed the hydrolysis of dipropyl phthalate, dibutyl phthalate, and dipentyl phthalate to the corresponding monoalkyl phthalate esters at low temperatures. The catalytic triad residues of DphB were proposed to be Ser159, Asp251, and His281.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Department of Environmental Biology, School of the Environment, Nanjing University, Nanjing, People's Republic of China.
authors.forename: Yiying
authors.initials: Y
authors.lastname: Jiao
authors.forename: Xu
authors.initials: X
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Xuewei
authors.initials: X
authors.lastname: Liao
authors.forename: Lin
authors.initials: L
authors.lastname: Xiao
authors.forename: Aijun
authors.initials: A
authors.lastname: Miao
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Liuyan
authors.initials: L
authors.lastname: Yang
mesh_terms: D018441:Biofilms; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D015723:Gene Library; D056186:Metagenomics; D062065:Wastewater / Q000382:microbiology*
chemical_list: D002265:Carboxylic Ester Hydrolases; D062065:Wastewater; C033216:phthalate ester hydrolase