title: A simple and sensitive resonance Rayleigh scattering method for determination of As(III) using aptamer-modified nanogold as a probe.
abstract: A simple and selective aptamer (ssDNA)-modified nanogold probe (AussDNA) was prepared for the determination of trace As(III) in HEPES buffer solution (pH 8.2) containing 0.05 mol/L NaCl. The method coupled the aptamer reaction of AussDNA-As(III) and the resonance Rayleigh scattering (RRS) of nanogold aggregations at 278 nm. When the As(III) concentration increased, the RRS intensity at 278 nm increased to form more nanogold aggregation and a stable As(III)-ssDNA complex. Under selected conditions, the increased RRS intensity (ΔI) was linear to the concentration of As(III) in the range 3.8-230.4 ng/mL, with a detection limit of 1.9 ng/mL. This RRS method was applied to detect As(III) in water samples, with simplicity, sensitivity and selectivity.
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin, 541004, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Tang
authors.forename: Guiqing
authors.initials: G
authors.lastname: Wen
authors.forename: Aihui
authors.initials: A
authors.lastname: Liang
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001151:Arsenic / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D012542:Scattering, Radiation; D013057:Spectrum Analysis
chemical_list: D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D006046:Gold; D001151:Arsenic
keywords: Aptamer; As(III); Nanogold; Resonance Rayleigh scattering