title: Development of PLGA-mannosamine nanoparticles as oral protein carriers.
abstract: Here we report the development of polymeric nanoparticles, made of poly(lactide-co-glycolide) (PLGA) chemically modified with mannosamine (MN), intended to specifically interact with the intestinal mucosa and facilitate the intestinal transport of proteins. PLGA-MN nanoparticles displayed nanometric size and a negative zeta potential, which was lower than that of the PLGA nanoparticles. This correlate well with the preferential location of the MN group on the nanoparticles surface obtained by X-ray photoelectron spectroscope (XPS). The presence of MN groups in the polymer chain led to a different surface morphology noted by SEM, an increase of the encapsulation of model proteins, and to help stabilizing the nanoparticles in simulated intestinal fluids. Furthermore, the MN modification significantly enhanced the nanoparticle's interaction with the epithelial cells in human intestinal follicle-associated epithelium cell culture model. Overall, the MN modification significantly modifies the properties of PLGA nanoparticles making them more suitable as nanocarriers for oral protein delivery.
authors.affiliation: CIMUS Research Institute, Campus Vida - University of Santiago de Compostela (USC) , Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Alonso-Sande
authors.forename: Anne
authors.initials: A
authors.lastname: des Rieux
authors.forename: Virginie
authors.initials: V
authors.lastname: Fievez
authors.forename: Bruno
authors.initials: B
authors.lastname: Sarmento
authors.forename: Araceli
authors.initials: A
authors.lastname: Delgado
authors.forename: Carmen
authors.initials: C
authors.lastname: Evora
authors.forename: Carmen
authors.initials: C
authors.lastname: Remuñán-López
authors.forename: Véronique
authors.initials: V
authors.lastname: Préat
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Alonso
mesh_terms: D000284:Administration, Oral; D002478:Cells, Cultured; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry; D004847:Epithelial Cells / Q000737:chemistry / Q000378:metabolism; D006595:Hexosamines / Q000008:administration & dosage / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D010316:Particle Size; D011098:Polyglactin 910 / Q000008:administration & dosage / Q000737:chemistry*; D011506:Proteins / Q000008:administration & dosage* / Q000737:chemistry; D013499:Surface Properties
chemical_list: D004337:Drug Carriers; D006595:Hexosamines; D011506:Proteins; C008322:mannosamine; D011098:Polyglactin 910