title: Protein arrays as tool for studies at the host-pathogen interface.
abstract: Pathogens and parasites encode a wide spectrum of multifunctional proteins interacting to and modifying proteins in host cells. However, the current lack of a reliable method to unveil the protein-protein interactions (PPI) at the host-pathogen interface is retarding our understanding of many important pathogenic processes. Thus, the identification of proteins involved in host-pathogen interactions is important for the elucidation of virulence determinants, mechanisms of infection, host susceptibility and/or disease resistance. In this sense, proteomic technologies have experienced major improvements in recent years and protein arrays are a powerful and modern method for studying PPI in a high-throughput format. This review focuses on these techniques analyzing the state-of-the-art of proteomic technologies and their possibilities to diagnose and explore host-pathogen interactions. Major technical advancements, applications and protocol concerns are presented, so readers can appreciate the immense progress achieved and the current technical options available for studying the host-pathogen interface. Finally, future uses of this kind of array-based proteomic tools in the fight against infectious and parasitic diseases are discussed.
authors.affiliation: Parasitología Animal, Instituto de Recursos Naturales y Agrobiología de Salamanca (IRNASA, CSIC), Cordel de Merinas, 40-52, 37008 Salamanca, Spain.
authors.forename: Raúl
authors.initials: R
authors.lastname: Manzano-Román
authors.forename: Noelia
authors.initials: N
authors.lastname: Dasilva
authors.forename: Paula
authors.initials: P
authors.lastname: Díez
authors.forename: Verónica
authors.initials: V
authors.lastname: Díaz-Martín
authors.forename: Ricardo
authors.initials: R
authors.lastname: Pérez-Sánchez
authors.forename: Alberto
authors.initials: A
authors.lastname: Orfao
authors.forename: Manuel
authors.initials: M
authors.lastname: Fuentes
mesh_terms: D000818:Animals; D054884:Host-Pathogen Interactions; D006801:Humans; D007239:Infections / Q000378:metabolism*; D010272:Parasitic Diseases / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods*; D040901:Proteomics / Q000379:methods*
keywords: Diseases; Interactions; Interface; Pathogens; Protein arrays; Proteomics