title: [Study on rapid propagation of Scutellaria baicalensis variation].
abstract: OBJECTIVE
  To establish and optimize the technology of taking root and promoting seedlings of white flower Scutellaria baicalensis test tube plantlet, and provide the theory and technology base for efficient factorization production system of white flower Scutellaria baicalensis.
  
  
  METHODS
  Stem segments with axillary bud were cultured onto the different basic medium with different kinds and concentration of cytokinin and auxins to take root and produce strong seedling.
  
  
  RESULTS
  The suitable culture medium for taking root of white flower Scutellaria baicalensis was 1/2 MS (all substance reduced half) + IBA 0.02 mg/L + sucrose 2%, the induction rate of root was 100%; The best medium for promoting seedling was 1/2 MS (all substance reduced half) + PP333 0.2 mg/L + IBA 0.02 mg/L + sucrose 2%, the seedling was green, the internode was normal, and its growth was vigorous and healthy.
  
  
  CONCLUSIONS
  1/2 MS (all substance reduced half) culture medium and relatively low concentration of sucrose is beneficial to inducing roots; Media adding appropriate concentration of IBA can significantly increase the root induction rate, the seedling has many stout roots; PP333 has dwarfing effect on seedlings and suitable concentration of PP333 can significantly improve the quality of the plantlets. A good technology of taking root and producing strong test tube plantlets is established.
authors.affiliation: Department of Pharmacy, Gansu College of TCM, Lanzhou 730000, China. zhyh456789@163.com
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Zhang
authors.forename: Su-Fang
authors.initials: SF
authors.lastname: Gao
authors.forename: Hong-Gang
authors.initials: HG
authors.lastname: Chen
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Jin
mesh_terms: D002716:Chlormequat / Q000494:pharmacology; D003470:Culture Media; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development*; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development; D018547:Plant Stems / Q000187:drug effects / Q000254:growth & development; D031364:Scutellaria baicalensis / Q000187:drug effects / Q000254:growth & development*; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development; D013395:Sucrose / Q000737:chemistry / Q000494:pharmacology; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators; D013395:Sucrose; D002716:Chlormequat