title: Connective tissue reaction of rats to a new zinc-oxide-eugenol endodontic sealer.
abstract: The aim of this study was to evaluate the biocompatibility in rat subcutaneous connective tissue of a new zinc oxide endodontic sealer (Endomethasone N) compared to those provided by Endofill and Sealer 26. Polyethylene tubes containing the test materials were implanted into dorsal subcutaneous connective tissue of Wistar albino rats. After 7 and 42 days, the implants with the surrounding tissue were collected, fixed, and processed for histologic evaluation. Sections were evaluated for the presence of inflammatory cells (poly or monomorfonuclear), blood vessels, necrosis area, and thickness of fibrous capsule. Comparisons between groups and time-periods were performed with Kruskal-Wallis and Mann-Whitney U non-parametric tests for 5% significance level. No differences in the biocompatibility patterns among the materials for the 2 experimental periods were observed. Independently of the sealer, the tissue behavior showed a tendency to decrease the irritation effect over time. It can be concluded that all sealers are irritant, but its toxicity decreased with time. Endomethásone N showed biocompatible characteristics comparable with those provided by Endofill and Sealer 26.
authors.affiliation: Integrated College from North of Minas Gerais, FUNORTE, Florianópolis/SC, Brazil.
authors.forename: Karen Melina
authors.initials: KM
authors.lastname: Trichês
authors.forename: Jacy Simi
authors.initials: JS
authors.lastname: Júnior
authors.forename: João Batista
authors.initials: JB
authors.lastname: Calixto
authors.forename: Ricardo
authors.initials: R
authors.lastname: Machado
authors.forename: Tiago Pereira
authors.initials: TP
authors.lastname: Rosa
authors.forename: Emmanuel João Nogueira Leal
authors.initials: EJ
authors.lastname: Silva
authors.forename: Luiz Pascoal
authors.initials: LP
authors.lastname: Vansan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D001729:Bismuth / Q000494:pharmacology; D002126:Calcium Hydroxide / Q000494:pharmacology; D003238:Connective Tissue / Q000187:drug effects*; D003907:Dexamethasone / Q000494:pharmacology*; D004338:Drug Combinations; D005557:Formaldehyde / Q000494:pharmacology*; D006854:Hydrocortisone / Q000494:pharmacology*; D007249:Inflammation / Q000139:chemically induced; D008297:Male; D008422:Materials Testing; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000494:pharmacology; D040521:Subcutaneous Tissue / Q000187:drug effects*; D013943:Thymol / Q000031:analogs & derivatives* / Q000494:pharmacology; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D004338:Drug Combinations; D012387:Root Canal Filling Materials; C080620:Sealer 26; D015035:Zinc Oxide-Eugenol Cement; C011244:corticosteroid methanetriol mixture; C074417:Endo-Fill; D005557:Formaldehyde; D013943:Thymol; D003907:Dexamethasone; D002126:Calcium Hydroxide; D001729:Bismuth; D006854:Hydrocortisone
keywords: Endomethásone N; biocompatibility; root canal sealer