title: Effects of botulinum toxin A on patient-specific keloid fibroblasts in vitro.
abstract: OBJECTIVE
  To test whether therapeutic effects of botulinum toxin A on patient- specific keloid tissue can be reproduced on the cellular level. Specifically, effects on cell proliferation and expression of growth factors and cytokines relevant for wound healing were to be tested.
  
  
  METHODS
  Experimental study including patient specific cell cultures of keloids.
  
  
  METHODS
  Patient-specific keloid tissue was tested in a cell culture model for effects of botulinum toxin incubation on cell proliferation and expression of the following cytokines and growth factors: IL-6 (interleukin-6), VEGF (vascular endothelial growth factor), TGF-β (transforming growth factor-β).
  
  
  RESULTS
  None of the tested parameters of human keloid tissue were affected by botulinum toxin A incubation.
  
  
  CONCLUSIONS
  The present study does not add evidence to suggest a significant therapeutic role of botulinum toxin injections for the treatment of keloids.
  
  
  METHODS
  N/A.
authors.affiliation: Laboratory of Facial Plastic Surgery Research, Department of Otorhinolaryngology, University of Regensburg, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Haubner
authors.forename: Michaela
authors.initials: M
authors.lastname: Leyh
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Ohmann
authors.forename: Haneen
authors.initials: H
authors.lastname: Sadick
authors.forename: Holger G
authors.initials: HG
authors.lastname: Gassner
mesh_terms: D000293:Adolescent; D000328:Adult; D019274:Botulinum Toxins, Type A / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis / Q000378:metabolism; D007627:Keloid / Q000473:pathology* / Q000601:surgery; D008297:Male; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D053773:Transforming Growth Factor beta1 / Q000032:analysis / Q000378:metabolism; D042442:Vascular Endothelial Growth Factors / Q000032:analysis / Q000378:metabolism; D055815:Young Adult
chemical_list: D016207:Cytokines; D015850:Interleukin-6; D053773:Transforming Growth Factor beta1; D042442:Vascular Endothelial Growth Factors; D019274:Botulinum Toxins, Type A
keywords: Keloids; botulinum toxin; cytokines; fibroblasts