title: Detection of genetically modified soybean in crude soybean oil.
abstract: In order to detect presence and quantity of Roundup Ready (RR) soybean in crude oil extracted from soybean seed with a different percentage of GMO seed two extraction methods were used, CTAB and DNeasy Plant Mini Kit. The amplifications of lectin gene, used to check the presence of soybean DNA, were not achieved in all CTAB extracts of DNA, while commercial kit gave satisfactory results. Comparing actual and estimated GMO content between two extraction methods, root mean square deviation for kit is 0.208 and for CTAB is 2.127, clearly demonstrated superiority of kit over CTAB extraction. The results of quantification evidently showed that if the oil samples originate from soybean seed with varying percentage of RR, it is possible to monitor the GMO content at the first stage of processing crude oil.
authors.affiliation: Institute of Field and Vegetable Crops, Maksima Gorkog 30, Novi Sad, Serbia. Electronic address: zorica.nikolic@ifvcns.ns.ac.rs.
authors.forename: Zorica
authors.initials: Z
authors.lastname: Nikolić
authors.forename: Ivana
authors.initials: I
authors.lastname: Vasiljević
authors.forename: Gordana
authors.initials: G
authors.lastname: Zdjelar
authors.forename: Vuk
authors.initials: V
authors.lastname: Ðorđević
authors.forename: Maja
authors.initials: M
authors.lastname: Ignjatov
authors.forename: Dušica
authors.initials: D
authors.lastname: Jovičić
authors.forename: Dragana
authors.initials: D
authors.lastname: Milošević
mesh_terms: D018556:Crops, Agricultural / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018744:DNA, Plant / Q000302:isolation & purification / Q000378:metabolism; D004351:Drug Resistance; D005506:Food Contamination; D005511:Food Handling; D005513:Food Inspection / Q000379:methods; D005515:Food Labeling / Q000331:legislation & jurisprudence; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D006540:Herbicides / Q000494:pharmacology; D037121:Plant Lectins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D055771:Serbia; D013024:Soybean Oil / Q000737:chemistry*; D030262:Soybean Proteins / Q000235:genetics / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D000097797:Glyphosate; D010835:Phytohemagglutinins
chemical_list: D018744:DNA, Plant; D005998:Glycine; D006540:Herbicides; D037121:Plant Lectins; D013024:Soybean Oil; D030262:Soybean Proteins; D000097797:Glyphosate; C028824:soybean lectin; D010835:Phytohemagglutinins
keywords: Crude oil; GMO; Soybean