title: In vitro study of interactions between silicon-containing nanoparticles and human peripheral blood leukocytes.
abstract: The effects of silicon dioxide-based nanoparticles on the viability and proliferative activity of human peripheral blood cultured lymphocytes were studied. All nanoparticles in a concentration of 100 μg/ml produced a significant cytotoxic effect, its intensity depending on particles' structure: SiO2 nanoparticles were least toxic, while Ce3(+)-intercaled montmorillonite nanoparticles were most toxic. The cells died mainly by apoptosis and postapoptotic necrosis. Incubation with nanoparticles in a concentration of 100 μg/ml for 72 h caused death of all phytohemagglutinin-activated lymphocytes, while in concentrations of 1 and 10 μg/ml the nanoparticles had no effect of proliferative activity of cells. The results suggest that the effects of nanoparticles on cells are determined by the nanoparticle concentration and size, as well as by their structure.
authors.affiliation: Laboratory of Cell Physiology, Institute of Biomedical Problems, Russian Academy of Sciences; Institute of Applied Nanotechnology, Moscow, Russia; Salvatore Mauggiery Foundation, Pavia, Italy. andreeva_er@mail.ru.
authors.forename: E R
authors.initials: ER
authors.lastname: Andreeva
authors.forename: E G
authors.initials: EG
authors.lastname: Rudimov
authors.forename: A N
authors.initials: AN
authors.lastname: Gornostaeva
authors.forename: V I
authors.initials: VI
authors.lastname: Beklemyshev
authors.forename: I I
authors.initials: II
authors.lastname: Makhonin
authors.forename: U O G
authors.initials: UO
authors.lastname: Maugeri
authors.forename: L B
authors.initials: LB
authors.lastname: Buravkova
mesh_terms: D001546:Bentonite / Q000032:analysis; D016923:Cell Death / Q000502:physiology; D002478:Cells, Cultured; D002563:Cerium; D005434:Flow Cytometry; D005452:Fluoresceins; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism*; D010835:Phytohemagglutinins; D012822:Silicon Dioxide / Q000032:analysis*; D012834:Silver; D018709:Statistics, Nonparametric; D013388:Succinimides
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D010835:Phytohemagglutinins; D013388:Succinimides; D001546:Bentonite; D002563:Cerium; D012834:Silver; D012822:Silicon Dioxide