title: Dimethoate induces kidney dysfunction, disrupts membrane-bound ATPases and confers cytotoxicity through DNA damage. Protective effects of vitamin E and selenium.
abstract: Dimethoate (DM) is an organophosphate insecticide widely used in agriculture and industry and has toxic effects on non-target organisms especially mammalian. However, we still know little about DM-induced kidney injury and its alleviation by natural antioxidants. In the present study, selenium (Se), vitamin E, DM, Se+DM, vitamin E+DM, Se+vitamin E+DM were given to adult rats for 4 weeks. Plasma creatinine and uric acid, kidney MDA, PC, H2O2 and AOPP levels were higher, while Na(+)-K(+)-ATPase and LDH values were lower in the DM group than those of controls. A smear without ladder formation on agarose gel was shown in the DM group, indicating random DNA degradation and DM-induced genotoxicity. A decrease in kidney GSH, NPSH and plasma urea levels and an increase in GPx, SOD and catalase activities were observed in the DM group when compared to those of controls. Plasma cystatin C levels increased, indicating a decrease in glomerular filtration rate. When Se or vitamin E was added through diet, the biochemical parameters cited above were partially restored in Se+DM and vitamin E+DM than DM group. The joint effect of Se and vitamin E was more powerful against DM-induced oxidative stress and kidney dysfunction. The changes in biochemical parameters were substantiated by histological data. In conclusion, our results indicated a possible mechanism of DM-induced nephrotoxicity, where renal genotoxicity was noted, membrane-bound ATPases and plasma biomarkers were disturbed. Se and vitamin E ameliorated the toxic effects of this pesticide in renal tissue suggesting their role as potential antioxidants.
authors.affiliation: Animal Physiology Laboratory, Sfax Faculty of Science, Sfax University, BP1171, 3000, Sfax, Tunisia.
authors.forename: Ibtissem
authors.initials: I
authors.lastname: Ben Amara
authors.forename: Aida
authors.initials: A
authors.lastname: Karray
authors.forename: Ahmed
authors.initials: A
authors.lastname: Hakim
authors.forename: Yassine
authors.initials: Y
authors.lastname: Ben Ali
authors.forename: Afef
authors.initials: A
authors.lastname: Troudi
authors.forename: Nejla
authors.initials: N
authors.lastname: Soudani
authors.forename: Tahia
authors.initials: T
authors.lastname: Boudawara
authors.forename: Khaled Mounir
authors.initials: KM
authors.lastname: Zeghal
authors.forename: Najiba
authors.initials: N
authors.lastname: Zeghal
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002462:Cell Membrane / Q000201:enzymology* / Q000473:pathology; D003603:Cytotoxins / Q000009:adverse effects* / Q000494:pharmacology; D004249:DNA Damage; D004117:Dimethoate / Q000009:adverse effects* / Q000494:pharmacology; D005260:Female; D007306:Insecticides / Q000009:adverse effects* / Q000494:pharmacology; D007674:Kidney Diseases / Q000139:chemically induced / Q000188:drug therapy / Q000201:enzymology / Q000401:mortality; D010088:Oxidoreductases; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D003603:Cytotoxins; D007306:Insecticides; D014810:Vitamin E; D010088:Oxidoreductases; D000251:Adenosine Triphosphatases; D012643:Selenium; D004117:Dimethoate