title: Quantification of secretory leukocyte protease inhibitor (SLPI) in oral gargle specimens collected using mouthwash.
abstract: BACKGROUND
  Secretory leukocyte protease inhibitor (SLPI) is an innate immunity-associated protein known to inhibit HIV transmission, and is thought to inhibit a variety of infectious agents, including human papillomaviruses (HPVs). We aimed to optimize an established ELISA-based SLPI quantification assay for use with oral gargle specimens collected using mouthwash, and to assess preliminary associations with age, smoking status, and alcohol intake.
  
  
  METHODS
  Oral gargle supernatants from 50 individuals were used to optimize the Human SLPI Quantikine ELISA Kit. Sample suitability was assessed and quality control analyses were conducted.
  
  
  RESULTS
  Salivary SLPI was successfully recovered from oral gargles with low intra-assay and high inter-individual variability. Initial measurements showed that salivary SLPI varied considerably across individuals, and that SLPI was inversely associated with age.
  
  
  CONCLUSIONS
  This optimized assay can be used to examine the role of SLPI in the acquisition of oral HPV and other infections.
authors.affiliation: Department of Cancer Epidemiology, Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, USA; Center for Infection Research in Cancer, Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, USA.
authors.forename: Christine M
authors.initials: CM
authors.lastname: Pierce Campbell
authors.forename: Wei
authors.initials: W
authors.lastname: Guan
authors.forename: Robert
authors.initials: R
authors.lastname: Sprung
authors.forename: John M
authors.initials: JM
authors.lastname: Koomen
authors.forename: Michael T
authors.initials: MT
authors.lastname: O'Keefe
authors.forename: Donna J
authors.initials: DJ
authors.lastname: Ingles
authors.forename: Martha
authors.initials: M
authors.lastname: Abrahamsen
authors.forename: Anna R
authors.initials: AR
authors.lastname: Giuliano
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007113:Immunity, Innate; D008297:Male; D009055:Mouth / Q000201:enzymology*; D009067:Mouthwashes; D015588:Observer Variation; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D011446:Prospective Studies; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012463:Saliva / Q000201:enzymology*; D053481:Secretory Leukocyte Peptidase Inhibitor / Q000032:analysis*; D013048:Specimen Handling
chemical_list: D009067:Mouthwashes; D011933:Reagent Kits, Diagnostic; C506814:SLPI protein, human; D053481:Secretory Leukocyte Peptidase Inhibitor
keywords: CV; HNC; HPV; Immune system protein; Innate immunity; OD; Oral disease; Oral gargle; SLPI; Secretory leukocyte protease inhibitor; coefficients of variation.; head and neck cancer; human papillomavirus; optical density; secretory leukocyte protease inhibitor