title: Vitamin C and oxidative stress on cultured human keratinocytes.
abstract: The aim of this research was to study the hypoxia-induced oxidative stress on cultured human keratinocytes. Control and cultured human keratinocytes were treated with vitamin C, and were then assessed by determining the concentration of malonyl dialdehyde (a final product of lipid peroxidation) existing in the cultured environment. Forty-eight flasks with culture of keratinocytes in confluent layer were divided into four groups: one control and one experimental group treated with vitamin C, and one control and one experimental group without vitamin C. All the cultures were sampled for malonyl dialdehyde assessment immediately after the end of a 30 min period of hypoxia. The statistical analysis of the results showed that vitamin C was not an effective protective agent against oxidative stress caused by hypoxia.
authors.affiliation: Division of Plastic Surgery, Department of Surgery, Federal University of São Paulo, São Paulo, Brazil.
authors.forename: Laélcio Lins Ramos
authors.initials: LL
authors.lastname: Santos
authors.forename: Alfredo
authors.initials: A
authors.lastname: Gragnani
authors.forename: Lydia Masako
authors.initials: LM
authors.lastname: Ferreira
authors.forename: Ivone
authors.initials: I
authors.lastname: da Silva Duarte
authors.forename: Ronaldo Gonçalves
authors.initials: RG
authors.lastname: Silva
keywords: Cultured cells; Free radicals; Human keratinocytes; Oxidative stress; Vitamin C