title: Analysis of mitochondrial DNA HVR1 haplotype of pure-bred domestic dogs in Japan.
abstract: To develop DNA markers for forensic analysis, we examined the hypervariable region 1 (HVR1) sequences of 447 pure-bred domestic dogs (Canis lupus familiaris) that had been bred and raised in Japan. HVR1 is a 660-bp stretch of mitochondrial (mt) DNA. Among the 447 HVR1 sequences examined, we identified 58 haplotypes from 47 single nucleotide polymorphisms (SNPs) and two insertion-deletion (InDel) polymorphisms. The haplotype diversity inferred from inter-breed analysis (N=154, 88 breeds) was 0.929±0.011. Intra-breed analysis showed that the haplotype diversity of Golden Retrievers (N=53), Labrador Retrievers (N=67), Miniature Dachshunds (N=61), Toy Poodles (N=62), and Welsh Corgis (N=50) was 0.624±0.052, 0.722±0.029, 0.922±0.010, 0.877±0.020, and 0.443±0.084, respectively. The results of this genotype analysis were used to construct a dataset consisting of dog mtDNA HVR1 sequences for use in forensic applications in Japan.
authors.affiliation: Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan.
authors.forename: Sho
authors.initials: S
authors.lastname: Sugiyama
authors.forename: Yong Hwa
authors.initials: YH
authors.lastname: Chong
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shito
authors.forename: Manami
authors.initials: M
authors.lastname: Kasuga
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kawakami
authors.forename: Chihiro
authors.initials: C
authors.lastname: Udagawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Aoki
authors.forename: Makoto
authors.initials: M
authors.lastname: Bonkobara
authors.forename: Shuichi
authors.initials: S
authors.lastname: Tsuchida
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sakamoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Okuda
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nagai
authors.forename: Toshinori
authors.initials: T
authors.lastname: Omi
mesh_terms: D000818:Animals; D022801:Complementarity Determining Regions / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D004285:Dogs / Q000235:genetics*; D053803:Forensic Genetics / Q000379:methods*; D014644:Genetic Variation; D006239:Haplotypes; D007564:Japan; D018895:Microsatellite Repeats / Q000235:genetics; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D022801:Complementarity Determining Regions; D017931:DNA Primers; D004272:DNA, Mitochondrial
keywords: Domestic dog; Forensic science; Haplotype; Hypervariable region 1; Individual identification; Mitochondrial DNA