title: Molecular characterization of Cryptosporidium spp. from foals in Italy.
abstract: Fourteen Cryptosporidium isolates from hospitalized foals were genotyped and subtyped using PCR-RFLP analysis of the 18S rDNA. Cryptosporidium parvum and Cryptosporidium horse genotype were detected in 3 and 11 stool specimens, respectively. Sequences of the gp60 gene of Cryptosporidium horse genotype allowed identification of the subtype family VIaA15G4, defining a novel microsatellite pattern within horse subtype VIa. Cryptosporidium horse genotype has only been described occasionally worldwide and this is the first time it has been identified in foals from Italy.
authors.affiliation: Department of Veterinary Medical Sciences, Alma Mater Studiorum, Bologna University, Via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy. Electronic address: monica.caffara@unibo.it.
authors.forename: Monica
authors.initials: M
authors.lastname: Caffara
authors.forename: Silvia
authors.initials: S
authors.lastname: Piva
authors.forename: Federica
authors.initials: F
authors.lastname: Pallaver
authors.forename: Eleonora
authors.initials: E
authors.lastname: Iacono
authors.forename: Roberta
authors.initials: R
authors.lastname: Galuppi
mesh_terms: D000818:Animals; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016785:Cryptosporidium parvum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000235:genetics / Q000378:metabolism; D005243:Feces / Q000469:parasitology; D017125:Genes, Protozoan; D005838:Genotype; D006734:Horse Diseases / Q000453:epidemiology / Q000469:parasitology*; D006736:Horses; D007558:Italy / Q000453:epidemiology; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S
keywords: 18S rRNA; Cryptosporidium horse genotype; Cryptosporidium parvum; Equine; gp60