title: Determination of different forms of aminothiols in red blood cells without washing erythrocytes.
abstract: Detection and quantification of different aminothiols forms (reduced and total) in biological fluids are important for the investigation of oxidative stress-related diseases and cell homeostasis study. The aim of this study was to optimize a HPLC method in order to determine both reduced and total thiol forms in red blood cells (RBC) at low temperature without washing erythrocytes. Analytical recoveries for total and reduced thiols were 91.6-98.5 and 94.9-98.2% respectively. The relative standard deviations intra-assay for total and reduced thiols were 1.14-3.64 and 0.83-2.3% respectively and the relative standard deviations inter-assay for total and reduced thiols were 1.12-3.54 and 0.84-2.03%, respectively. This method allows specific analysis of the aminothiol state inside the RBC, as a model of intracellular metabolism functioning.
authors.affiliation: CNR Institute of Clinical Physiology, CardioThoracic and Vascular Department, Niguarda Ca' Granda Hospital, Milan, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Dellanoce
authors.forename: Lorena
authors.initials: L
authors.lastname: Cozzi
authors.forename: Stefania
authors.initials: S
authors.lastname: Zuddas
authors.forename: Lorenza
authors.initials: L
authors.lastname: Pratali
authors.forename: Roberto
authors.initials: R
authors.lastname: Accinni
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004912:Erythrocytes / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D013438:Sulfhydryl Compounds / Q000097:blood* / Q000737:chemistry
chemical_list: D013438:Sulfhydryl Compounds
keywords: HPLC; red blood cells; redox state; reduced and total aminothiols