title: Novel quercetin derivatives in treatment of peroxynitrite-oxidized SERCA1.
abstract: Sarco/endoplasmic reticulum calcium ATP-ase (SERCA) is regulated by low concentrations of peroxynitrite and inhibited by high levels, as indicated in human diseases. We studied quercetin (Q) and its novel derivatives monochloropivaloylquercetin (MPQ) and chloronaphthoquinonequercetin (CHQ) as agents with expected preventive properties against peroxynitrite-induced SERCA impairment. Q and MPQ protected the SERCA1 against peroxynitrite induced activity decrease, while CHQ potentiated the inhibitory effect of peroxynitrite. Quercetin derivatives were found to be weaker antioxidants compared with Q, as indicated by their ability to scavenge peroxynitrite and prevent of SERCA1 carbonylation, both decreasing in the order (Q > MPQ > CHQ). Quantum-chemical values of theoretical parameter E HOMO also indicated lower antioxidant capacities for MPQ and CHQ. Prooxidant properties estimated by calculations of frontier molecular orbitals (E LUMO) correlated with experimentally determined SH-group decrease induced by the compounds studied. Both methods showed a decrease of prooxidant properties as follows: CHQ > MPQ > Q. In addition, experimentally measured half-wave potentials indicated stronger prooxidant properties of quercetin derivatives as compared to Q. More expressive alterations of conformation in the transmembrane region of SERCA1 induced by quercetin derivatives, as compared with Q, may at least partially correlate with their higher lipophilicities. The protective effects of Q and MPQ on different isoforms of SERCA activity may be useful in prevention and treatment of inflammation or muscle diseases. The inhibitory effect of CHQ on SERCA isoforms may be beneficial in therapeutic approaches aimed at anti-tumor treatment.
authors.affiliation: Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: Petronela
authors.initials: P
authors.lastname: Žižková
authors.forename: Dušan
authors.initials: D
authors.lastname: Blaškovič
authors.forename: Magdaléna
authors.initials: M
authors.lastname: Májeková
authors.forename: L'ubomír
authors.initials: L
authors.lastname: Švorc
authors.forename: Lucia
authors.initials: L
authors.lastname: Račková
authors.forename: L'ubica
authors.initials: L
authors.lastname: Ratkovská
authors.forename: Miroslav
authors.initials: M
authors.lastname: Veverka
authors.forename: L'ubica
authors.initials: L
authors.lastname: Horáková
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D018482:Muscle, Skeletal / Q000187:drug effects / Q000201:enzymology; D010084:Oxidation-Reduction; D030421:Peroxynitrous Acid / Q000494:pharmacology*; D011499:Protein Processing, Post-Translational; D011794:Quercetin / Q000494:pharmacology*; D011817:Rabbits; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D030421:Peroxynitrous Acid; D011794:Quercetin; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D016650:Fluorescein-5-isothiocyanate