title: [Study on chemical components in yiqing capsule based on UPLC-ESI-MS-MS and FTIR and its anti-inflammatory activity in vitro].
abstract: OBJECTIVE
  To study the chemical components in Yiqing capsule by UPLC-ESI-MS-MS and its anti-inflammatory activity in vitro.
  
  
  METHODS
  The mass spectrometer equipped with an eletrospray ion source in positive ion mode was used and data of mass spectrum and mass/mass spectrum were analyzed; FTIR was used to detect the characteristic spectra of ten bcathes of Yiqing capsule. Compared the spectra by principal component analysis and cluster analysis; RAW264.7 cell model induced by LPS was established to study the inhibitory effect of Yiqing capsule on the release of NO.
  
  
  RESULTS
  Totally twelve chemical components were analyzed by the established UPLC-ESI-MS-MS method; The results of the principal component analysis and the cluster analysis indicated that there were significant differences between the sample (batch number: 120703) and other different batches of samples; Yiqing capsule could inhibit the release of NO induced by LPS in a dose-dependent manner at the concentration of 1 - 100 micromol/L.
  
  
  CONCLUSIONS
  UPLC-ESI-MS-MS and FTIR which are rapid, convenient and effective, can be used for quality control of Yiping capsule; Yiqing capsule exhibites anti-inflammatory activity in vitro.
authors.affiliation: Guangdong College of Pharmacy, Guangzhou 510006, China. luolan1105@126.com
authors.forename: Lan
authors.initials: L
authors.lastname: Luo
authors.forename: Shu-Mei
authors.initials: SM
authors.lastname: Wang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D002214:Capsules; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology*; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D025341:Principal Component Analysis; D011786:Quality Control; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000893:Anti-Inflammatory Agents; D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D009569:Nitric Oxide