title: β-Actin knock-out mouse embryonic fibroblasts show increased expression of LIM-, CH-, EFh-domain containing proteins with predicted common upstream regulators.
abstract: β-actin depletion from mouse embryonic fibroblasts results in an altered transcriptional response rendering these cells a myofibroblast like phenotype. The proteins and upstream regulatory factors responsible for this acquired phenotype, with prominent focal adhesions and stress fibres, are unknown. Data-mining of the changed proteome revealed that actin binding proteins associated with stress fiber or focal adhesion formation are overexpressed in the β-actin knock-out cells and that many of these contain CH-, LIM- or EFh- domains. Furthermore in silico analysis predicts potential common upstream regulators that may, at least partly, coordinate the altered transcriptional response.
authors.affiliation: Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University, Ghent.
authors.forename: Christophe
authors.initials: C
authors.lastname: Ampe
authors.forename: Jelle
authors.initials: J
authors.lastname: Libbrecht
authors.forename: Marleen
authors.initials: M
authors.lastname: Van Troys
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000199:Actins / Q000172:deficiency* / Q000378:metabolism; D000818:Animals; D003598:Cytoskeletal Proteins / Q000737:chemistry* / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D060588:LIM Domain Proteins / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D017434:Protein Structure, Tertiary; D020543:Proteome / Q000378:metabolism; D040901:Proteomics; D012045:Regulatory Sequences, Nucleic Acid; D012984:Software; D022502:Stress Fibers / Q000378:metabolism
chemical_list: D000199:Actins; D003598:Cytoskeletal Proteins; D060588:LIM Domain Proteins; D020543:Proteome
keywords: CH-domain; LIM-domain; actin cytoskeleton; stress fiber; transcription factor