title: Isolation of RNA from equine peripheral blood cells: comparison of methods.
abstract: Gene expression studies in equine research involve the use of whole blood samples as a vital source of RNA. To determine the optimal method for RNA isolation from equine whole blood, we compared three RNA isolation strategies using different commercially available kits to evaluate the yield and quality of equine RNA. All 3 methods produced RNA with high quality. Though it did not produce the highest yield, combining the quality, yield and the need for the downstream application in our project, LeukoLOCK™ total RNA isolation system was the best RNA extraction method.
authors.affiliation: Department of Clinical Studies, University of Pennsylvania School of Veterinary Medicine, New Bolton Center Campus, 382 West Street Road, Kennett Square, Kragujevac, PA 19348 USA.
authors.forename: Zibin
authors.initials: Z
authors.lastname: Jiang
authors.forename: Cornelius E
authors.initials: CE
authors.lastname: Uboh
authors.forename: Jinwen
authors.initials: J
authors.lastname: Chen
authors.forename: Lawrence R
authors.initials: LR
authors.lastname: Soma
keywords: Blood; Equine; LeukoLOCK™; Method comparison; RNA extraction