title: Surface remineralization potential of casein phosphopeptide-amorphous calcium phosphate on enamel eroded by cola-drinks: An in-situ model study.
abstract: OBJECTIVE
  The aim of this study was to investigate the remineralization potential of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) on enamel eroded by cola drinks.
  
  
  METHODS
  A total of 30 healthy subjects were selected from a random sample of 1200 children and divided into two groups of 15 each wherein calcium and phosphorus analyses and scanning electron microscope (SEM) analysis was carried out to investigate the remineralization of enamel surface. A total of 30 non-carious premolar teeth were selected from the human tooth bank (HTB) to prepare the in-situ appliance. Three enamel slabs were prepared from the same. One enamel slab was used to obtain baseline values and the other two were embedded into the upper palatal appliances prepared on the subjects' maxillary working model. The subjects wore the appliance after which 30 ml cola drink exposure was given. After 15 days, the slabs were removed and subjected to respective analysis.
  
  
  METHODS
  Means of all the readings of soluble calcium and phosphorous levels at baseline,post cola-drink exposure and post cpp-acp application were subjected to statistical analysis SPSS11.5 version. Comparison within groups and between groups was carried out using ANOVA and F-values at 1% level of significance.
  
  
  RESULTS
  Decrease in calcium solubility of enamel in the CPP-ACP application group as compared to post-cola drink exposure group (P < 0.05) was seen. Distinctive change in surface topography of enamel in the post-CPP-ACP application group as compared to post-cola drink exposure group was observed.
  
  
  CONCLUSIONS
  CPP-ACP significantly promoted remineralization of enamel eroded by cola drinks as revealed by significant morphological changes seen in SEM magnification and spectrophotometric analyses.
authors.affiliation: Department of Paedodontics and Preventive Dentistry, Punjab Government Dental College and Hospital, Amritsar, India.
authors.forename: Navneet
authors.initials: N
authors.lastname: Grewal
authors.forename: Vinod
authors.initials: V
authors.lastname: Kudupudi
authors.forename: Sukrit
authors.initials: S
authors.lastname: Grewal
keywords: Casein phosphopeptide-amorphous calcium phosphate; in-situ model; remineralization potential; spectrophotometric analysis and scanning electron microscope