title: PPARalpha regulates the production of serum Vanin-1 by liver.
abstract: The membrane-bound Vanin-1 pantetheinase regulates tissue adaptation to stress. We investigated Vnn1 expression and its regulation in liver. Vnn1 is expressed by centrolobular hepatocytes. Using novel tools, we identify a soluble form of Vnn1 in mouse and human serum and show the contribution of a cysteine to its catalytic activity. We show that liver contributes to Vanin-1 secretion in serum and that PPARalpha is a limiting factor in serum Vnn1 production. Functional PPRE sites are identified in the Vnn1 promoter. These results indicate that serum Vnn1 might be a reliable reporter of PPARalpha activity in liver.
authors.affiliation: Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille University, UM2, Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, Marseille, France; Centre National de la Recherche Scientifique (CNRS), UMR7280, Marseille, France.
authors.forename: Samuel
authors.initials: S
authors.lastname: Rommelaere
authors.forename: Virginie
authors.initials: V
authors.lastname: Millet
authors.forename: Thomas
authors.initials: T
authors.lastname: Gensollen
authors.forename: Christophe
authors.initials: C
authors.lastname: Bourges
authors.forename: Jérôme
authors.initials: J
authors.lastname: Eeckhoute
authors.forename: Nathalie
authors.initials: N
authors.lastname: Hennuyer
authors.forename: Eric
authors.initials: E
authors.lastname: Baugé
authors.forename: Lionel
authors.initials: L
authors.lastname: Chasson
authors.forename: Ivana
authors.initials: I
authors.lastname: Cacciatore
authors.forename: Bart
authors.initials: B
authors.lastname: Staels
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Pitari
authors.forename: Franck
authors.initials: F
authors.lastname: Galland
authors.forename: Philippe
authors.initials: P
authors.lastname: Naquet
mesh_terms: D000581:Amidohydrolases / Q000097:blood* / Q000235:genetics; D000818:Animals; D018938:Caco-2 Cells; D005260:Female; D058851:GPI-Linked Proteins / Q000097:blood / Q000235:genetics; D015870:Gene Expression; D005786:Gene Expression Regulation; D022781:Hepatocytes / Q000201:enzymology / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D047493:PPAR alpha / Q000378:metabolism*
chemical_list: D058851:GPI-Linked Proteins; D047493:PPAR alpha; D000581:Amidohydrolases; C022314:pantetheinase
keywords: Liver; PPARalpha; Pantetheinase; Vanin