title: The C-terminal cysteine of turbot Scophthalmus maximus translationally controlled tumour protein plays a key role in antioxidation and growth-promoting functions.
abstract: The translationally controlled tumour protein (TCTP) of turbot Scophthalmus maximus (SmTCTP) contains only one cysteine (Cys¹⁷⁰) at the C-terminal end. The biological role of this C-terminal Cys¹⁷⁰ in the antioxidation and growth-promoting functions of SmTCTP was examined by site-directed mutation of C170A (Cys¹⁷⁰ →Ala¹⁷⁰). It was found that C170A mutation not only obviously decreased the antioxidation capacity of the mutant-smtctp-transformed bacteria exposed to 0·22 mM hydrogen peroxide, but also significantly interrupted the normal growth and survival of the mutant-smtctp-transformed bacteria and flounder Paralichthys olivaceus gill (FG) cells, indicating a key role played by Cys¹⁷⁰ in the antioxidation and growth-promoting functions of SmTCTP. This study also suggested that the self-dimerization or dimerization with other interacting proteins is critical to the growth-promoting function of SmTCTP.
authors.affiliation: Department of Marine Biology, Ocean University of China, Qingdao, 266003, P. R. China.
authors.forename: Z-X
authors.initials: ZX
authors.lastname: Zhang
authors.forename: D-Y
authors.initials: DY
authors.lastname: Geng
authors.forename: Q
authors.initials: Q
authors.lastname: Han
authors.forename: S-D
authors.initials: SD
authors.lastname: Liang
authors.forename: H-R
authors.initials: HR
authors.lastname: Guo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000737:chemistry* / Q000235:genetics; D002460:Cell Line; D003545:Cysteine / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics; D029941:Fish Proteins / Q000737:chemistry* / Q000235:genetics; D005412:Flatfishes / Q000502:physiology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D017434:Protein Structure, Tertiary; D000090324:Tumor Protein, Translationally-Controlled 1
chemical_list: D000975:Antioxidants; D014408:Biomarkers, Tumor; D029941:Fish Proteins; D000090324:Tumor Protein, Translationally-Controlled 1; D003545:Cysteine
keywords: biological functions; flounder gill cells; overexpression; translationally controlled tumour protein