title: Oxidative stress is involved in fatigue induced by overnight deskwork as assessed by increase in plasma tocopherylhydroqinone and hydroxycholesterol.
abstract: In this study, we examined the relationship between fatigue and plasma concentrations of antioxidants and lipid peroxidation products. Fourteen healthy volunteers performed overnight desk work for 18h then took a nap for 4h. Participants answered questionnaires of subjective symptoms of fatigue (QSSF) and completed a self-assessment of fatigue using a visual analog scale (VAS). At each test time, they underwent a critical flicker frequency (CFF) test and blood samples were collected. Plasma levels of α-tocopherol (αT) decreased and α-tocopherylquinone (αTQ), the oxidation product of αT, increased. The ratio of 7β-hydroxycholesterol (7β-OHCh), the oxidation product of cholesterol, against total cholesterol increased until the end of experiment. αTQ levels correlated with VAS and QSSF scores. The ratio of 7β-OHCh to total cholesterol and the value of CFF showed a significant correlation. From these results, plasma levels of αTQ and 7β-OHCh are useful and objective indicators of fatigue induced by overnight deskwork.
authors.affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Osaka, Japan. Electronic address: mototada-shichiri@aist.go.jp.
authors.forename: Mototada
authors.initials: M
authors.lastname: Shichiri
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Harada
authors.forename: Noriko
authors.initials: N
authors.lastname: Ishida
authors.forename: Lilian Kaede
authors.initials: LK
authors.lastname: Komaba
authors.forename: Sunao
authors.initials: S
authors.lastname: Iwaki
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Hagihara
authors.forename: Etsuo
authors.initials: E
authors.lastname: Niki
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Yoshida
mesh_terms: D000328:Adult; D005221:Fatigue / Q000097:blood / Q000378:metabolism*; D006801:Humans; D006888:Hydroxycholesterols / Q000097:blood*; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D012647:Self-Assessment; D012892:Sleep Deprivation / Q000097:blood / Q000378:metabolism; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000097:blood; D014937:Work / Q000502:physiology*
chemical_list: D006888:Hydroxycholesterols; D014810:Vitamin E; C002421:tocopherylquinone
keywords: -tocopherol; 7β-OHCh; 7β-hydroxycholesterol; CFF; CoQ(10); Critical flicker frequency; Fatigue; HODE; Lipid peroxidation; Overnight deskwork; QSSF; UQ(10); UQ(10)H(2); VAS; coenzyme Q(10); critical flicker frequency; hydroxyoctadecadienoic acid; questionnaires of subjective symptoms of fatigue; ubiquinol-10; ubiquinone-10; visual analog scale; α-Tocopherylquinone; α-tocopherylquinone; αTQ; αTα