title: Acetone-butanol-ethanol production from corn stover pretreated by alkaline twin-screw extrusion pretreatment.
abstract: In this study, the alkaline twin-screw extrusion pretreated corn stover was subjected to enzymatic hydrolysis after washing. The impact of solid loading and enzyme dose on enzymatic hydrolysis was investigated. It was found that 68.2 g/L of total fermentable sugar could be obtained after enzymatic hydrolysis with the solid loading of 10 %, while the highest sugar recovery of 91.07 % was achieved when the solid loading was 2 % with the cellulase dose of 24 FPU/g substrate. Subsequently, the hydrolyzate was fermented by Clostridium acetobutylicum ATCC 824. The acetone-butanol-ethanol (ABE) production of the hydrolyzate was compared with the glucose, xylose and simulated hydrolyzate medium which have the same reducing sugar concentration. It was shown that 7.1 g/L butanol and 11.2 g/L ABE could be produced after 72 h fermentation for the hydrolyzate obtained from enzymatic hydrolysis with 6 % solid loading. This is comparable to the glucose and simulated hydrozate medium, and the overall ABE yield could reach 0.112 g/g raw corn stover.
authors.affiliation: Key Laboratory of Biofuel, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, Shandong, China, zhangyd@qibebt.ac.cn.
authors.forename: Yuedong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Tongang
authors.initials: T
authors.lastname: Hou
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Xindong
authors.initials: X
authors.lastname: Mu
authors.forename: Haisong
authors.initials: H
authors.lastname: Wang
mesh_terms: D000096:Acetone / Q000378:metabolism*; D000440:Butanols / Q000378:metabolism*; D046969:Clostridium acetobutylicum / Q000254:growth & development* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D000431:Ethanol / Q000378:metabolism*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D000440:Butanols; D003470:Culture Media; D000096:Acetone; D000431:Ethanol