title: Further characteristics of Arcanobacterium canis, a novel species of genus Arcanobacterium.
abstract: Comparable to previously conducted phenotypical and genotypical investigations characterizing Arcanobacterium canis, a newly described species with the type strain A. canis DSM 25104 isolated from an otitis externa of a dog, four additional A. canis strains isolated from infections of three dogs and one cat could reliably be identified by phenotypic properties, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and by sequencing the genomic targets 16S rDNA, 16S-23S rDNA intergenic spacer region, 23S rDNA, and the genes rpoB and gap. All four A. canis investigated in the present study were isolated from the infected animals together with several other bacterial species indicating that the pathogenic importance of A. canis remains unclear. However, the detection of peptidic spectra by MALDI-TOF MS and the presented phenotypic and genotypic approaches might help to identify A. canis in future and might elucidate the role this species plays in infections of dogs and cats.
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubert Str. 81, 35392 Gießen, Germany.
authors.forename: O
authors.initials: O
authors.lastname: Sammra
authors.forename: A
authors.initials: A
authors.lastname: Balbutskaya
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Hijazin
authors.forename: S
authors.initials: S
authors.lastname: Nagib
authors.forename: C
authors.initials: C
authors.lastname: Lämmler
authors.forename: A
authors.initials: A
authors.lastname: Abdulmawjood
authors.forename: E
authors.initials: E
authors.lastname: Prenger-Berninghoff
authors.forename: M
authors.initials: M
authors.lastname: Kostrzewa
authors.forename: M
authors.initials: M
authors.lastname: Timke
mesh_terms: D000193:Actinomycetales Infections / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D055667:Arcanobacterium / Q000145:classification* / Q000235:genetics*; D002371:Cat Diseases / Q000382:microbiology*; D002415:Cats; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D005838:Genotype; D008969:Molecular Sequence Data; D010032:Otitis Externa / Q000382:microbiology / Q000662:veterinary; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S
keywords: Arcanobacterium canis; Cat; Dog; MALDI-TOF; Molecular identification