title: TGIF1 splicing variant 8 is overexpressed in oral squamous cell carcinoma and is related to pathologic and clinical behavior.
abstract: OBJECTIVE
  Possible differences in splicing variants of TGIF1 in oral squamous cell carcinoma (OSCC) have not yet been reported. This study analyzed the expression levels of different splicing variants of the TGIF1 gene in OSCC compared with nontumoral epithelium (NT) and the relationship with clinical-pathologic features of tumors.
  
  
  METHODS
  Forty-eight frozen samples of OSCC and 17 of NT were analyzed using quantitative reverse transcription polymerase chain reaction.
  
  
  RESULTS
  TGIF1v2 and v8 are overexpressed in OSCC, whereas TGIF1v5 is underexpressed when compared with NT. Low TGIF1v8 expression was correlated with lower cellular differentiation, positive blood vascular invasion, advanced pathologic stage, and positive vascular lymphatic invasion of OSCC. TGIF1v8 is also related to overall survival over time, with lower values associated with an increased risk of cancer-related death.
  
  
  CONCLUSIONS
  These data suggest that alternative splicing of TGIF1 is deregulated in OSCC, with overexpression of some splicing variants, especially TGIF1v8, which is associated with advanced stages of OSCC.
authors.affiliation: Professor of Oral Pathology, Pathology and Legal Medicine Department, Federal University of Amazonas, Manaus, Amazonas; Associate Professor of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, São Paulo. Electronic address: tliborio@ufam.edu.br.
authors.forename: Tatiana Nayara
authors.initials: TN
authors.lastname: Libório
authors.forename: Elisa Napolitano
authors.initials: EN
authors.lastname: Ferreira
authors.forename: Flávia Caló
authors.initials: FC
authors.lastname: Aquino Xavier
authors.forename: Dirce Maria
authors.initials: DM
authors.lastname: Carraro
authors.forename: Luiz Paulo
authors.initials: LP
authors.lastname: Kowalski
authors.forename: Fernando Augusto
authors.initials: FA
authors.lastname: Soares
authors.forename: Fabio Daumas
authors.initials: FD
authors.lastname: Nunes
mesh_terms: D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D009367:Neoplasm Staging; D012097:Repressor Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors
chemical_list: D018398:Homeodomain Proteins; D012097:Repressor Proteins; C097504:TGIF1 protein, human