title: Inhibitory effects of LPA1 on cell motile activities stimulated by hydrogen peroxide and 2,3-dimethoxy-1,4-naphthoquinone in fibroblast 3T3 cells.
abstract: Reactive oxygen species (ROS) are known to mediate a variety of biological responses, including cell motility. Recently, we indicated that lysophosphatidic acid (LPA) receptor-3 (LPA3) increased cell motile activity stimulated by hydrogen peroxide. In the present study, we assessed the role of LPA1 in the cell motile activity mediated by ROS in mouse fibroblast 3T3 cells. 3T3 cells were treated with hydrogen peroxide and 2,3-dimethoxy-1,4-naphthoquinone (DMNQ) at concentrations of 0.1 and 1 μM for 48 h. In cell motility assays with Cell Culture Inserts, the cell motile activities of 3T3 cells treated with hydrogen peroxide and DMNQ were significantly higher than those of untreated cells. 3T3 cells treated with hydrogen peroxide and DMNQ showed elevated expression levels of the Lpar3 gene, but not the Lpar1 and Lpar2 genes. To investigate the effects of LPA1 on the cell motile activity induced by hydrogen peroxide and DMNQ, Lpar1-overexpressing (3T3-a1) cells were generated from 3T3 cells and treated with hydrogen peroxide and DMNQ. The cell motile activities stimulated by hydrogen peroxide and DMNQ were markedly suppressed in 3T3-a1 cells. These results suggest that LPA signaling via LPA1 inhibits the cell motile activities stimulated by hydrogen peroxide and DMNQ in 3T3 cells.
authors.affiliation: Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan.
authors.forename: Miku
authors.initials: M
authors.lastname: Hirane
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Araki
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.forename: Kanya
authors.initials: K
authors.lastname: Honoki
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Fukushima
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Tsujiuchi
mesh_terms: D016475:3T3 Cells; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D011756:Diphosphates / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D005990:Glycerol / Q000031:analogs & derivatives / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008246:Lysophospholipids / Q000494:pharmacology*; D051379:Mice; D009285:Naphthoquinones / Q000494:pharmacology*; D009447:Neuroblastoma / Q000473:pathology; D051381:Rats; D049368:Receptors, Lysophosphatidic Acid / Q000235:genetics / Q000378:metabolism
chemical_list: D011756:Diphosphates; D008246:Lysophospholipids; D009285:Naphthoquinones; D049368:Receptors, Lysophosphatidic Acid; C408642:diacylglycerol pyrophosphate; C063002:2,3-dimethoxy-1,4-naphthoquinone; D006861:Hydrogen Peroxide; D005990:Glycerol; C032881:lysophosphatidic acid
keywords: 2,3-dimethoxy-1,4-naphthoquinone; Cell motility; DGPP; DMNQ; Hydrogen peroxide; LPA; LPA receptor-1; LPA(1); PCR; ROS; RT; diactylglycerol pyrophosphate; lysophosphatidic acid; polymerase chain reaction; reactive oxygen species; reverse transcription