title: ELF magnetic fields do not affect cell survival and DNA damage induced by ultraviolet B.
abstract: We investigated whether extremely low frequency (ELF) magnetic field exposure has modification effects on cell survival after ultraviolet B (UV-B) irradiation and on repair process of DNA damage induced by UV-B irradiation in WI38VA13 subcloned 2RA and XP2OS(SV) cells. The ELF magnetic field exposure was conducted using a Helmholtz coil-based system that was designed to generate a sinusoidal magnetic field at 5 mT and 60 Hz. Cell survival was assessed by WST assay after UV-B irradiation at 20-80 J/m(2) , ELF magnetic field exposure for 24 h, followed by incubation for 48 h. DNA damage was assessed by quantification of cyclobutane pyrimidine dimer formation and 6-4 photoproduct formation using ELISA after UV-B irradiation at 20-80 J/m(2) followed by ELF magnetic field exposure for 24 h. No significant changes were observed in cell survival between ELF magnetic field and sham exposures. Similarly, DNA damage induced by UV-B irradiation did not change significantly following ELF magnetic field exposure. Our results suggest that ELF magnetic field exposure at 5 mT does not have modification effect on cell survival after UV-B irradiation and on repair process of DNA damage induced by UV-B irradiation.
authors.affiliation: Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Japan; Power Engineering R&D Center, Kansai Electric Power Company, Osaka, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Mizuno
authors.forename: Eijiro
authors.initials: E
authors.lastname: Narita
authors.forename: Masaru
authors.initials: M
authors.lastname: Yamada
authors.forename: Naoki
authors.initials: N
authors.lastname: Shinohara
authors.forename: Junji
authors.initials: J
authors.lastname: Miyakoshi
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D002999:Clone Cells / Q000166:cytology / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair / Q000528:radiation effects; D006801:Humans; D060526:Magnetic Fields; D011740:Pyrimidine Dimers / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D011740:Pyrimidine Dimers
keywords: 6-4 photoproduct; ELF magnetic field; UV-B; cyclobutane pyrimidine dimer; irradiation; ultraviolet B; xeroderma pigmentosum A