title: In vitrostudy on the feasibility of magnetic stent hyperthermia for the treatment of cardiovascular restenosis.
abstract: Thermal treatment or hyperthermia has received considerable attention in recent years due to its high efficiency, safety and relatively few side-effects. In this study, we investigated whether it was possible to utilize targeted thermal or instent thermal treatments for the treatment of restenosis following percutaneous transluminal coronary angioplasty (PTCA) through magnetic stent hyperthermia (MSH). A 316L stainless steel stent and rabbit vascular smooth muscle cells (VSMCs) were used in the present study, in which the inductive heating characteristics of the stent under alternative magnetic field (AMF) exposure, as well as the effect of MSH on the proliferation, apoptosis, cell cycle and proliferating cell nuclear antigen (PCNA) expression of the rabbit VSMCs, were evaluated. The results demonstrated that 316L stainless steel coronary stents possess ideal inductive heating characteristics under 300 kHz AMF exposure. The heating properties were shown to be affected by the field intensity of the AMF, as well as the orientation the stent axis. MSH had a significant effect on the proliferation and apoptosis of VSMCs, and the effect was temperature-dependent. While a mild temperature of 43°C demonstrated negligible effects on the growth of VSMCs, MSH treatment above 47°C effectively inhibited the VSMC proliferation and induced apoptosis. Furthermore, a 47°C treatment exhibited a significant and long-term inhibitory effect on VSMC migration. The results strongly suggested that MSH may be potentially applied in the clinic as an alternative approach for the prevention and treatment of restenosis.
authors.affiliation: Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan 410008; ; Institute of Medical Physics and Engineering, Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Department of Engineering Physics, Tsinghua University, Beijing 100084;
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Huan-Huan
authors.initials: HH
authors.lastname: Shi
authors.forename: LE
authors.initials: L
authors.lastname: Xie
authors.forename: Jing-Ding-Sha
authors.initials: JD
authors.lastname: Li
authors.forename: Wei-Chao
authors.initials: WC
authors.lastname: Kong
authors.forename: Jin-Tian
authors.initials: JT
authors.lastname: Tang
authors.forename: DA-Nian
authors.initials: DN
authors.lastname: Ke
authors.forename: Ling-Yun
authors.initials: LY
authors.lastname: Zhao
keywords: alternative magnetic field; magnetic stent hyperthermia; percutaneous transluminal coronary angioplasty; restenosis; vascular smooth muscle cells