title: The complete genome sequences of two isolates of potato black  ringspot virus and their relationship to other isolates  and nepoviruses.
abstract: The complete nucleotide sequences of RNA 1 and RNA 2 of the nepovirus potato black ringspot virus (PBRSV) from two different isolates were determined, as well as partial sequences from two additional isolates. RNA1 is 7,579-7,598 nucleotides long and contains one single open reading frame (ORF), which is translated into a large polyprotein with 2,325 amino acids and a molecular weight of 257 kDa. The complete sequence of RNA2 ranges from 3857 to 3918 nt between the different isolates. It encodes a polyprotein of 1079-1082 amino acids with a molecular weight of 120 kDa. Sequence comparison using the Pro-Pol region and CP showed that all four isolates formed two distinct groups, corresponding to potato and arracacha, that were closely related to each other and also to tobacco ringspot virus (TRSV). Comparing our data to those obtained with other nepoviruses, our results confirm that PBRSV belongs to a distinct species and is a member of subgroup A in the genus Nepovirus based on its RNA2 size, genome organization, and nucleotide sequence.
authors.forename: R Souza
authors.initials: RS
authors.lastname: Richards
authors.forename: I P
authors.initials: IP
authors.lastname: Adams
authors.forename: J F
authors.initials: JF
authors.lastname: Kreuze
authors.forename: J
authors.initials: J
authors.lastname: De Souza
authors.forename: W
authors.initials: W
authors.lastname: Cuellar
authors.forename: A M
authors.initials: AM
authors.lastname: Dullemans
authors.forename: R A A
authors.initials: RA
authors.lastname: Van Der Vlugt
authors.forename: R
authors.initials: R
authors.lastname: Glover
authors.forename: U
authors.initials: U
authors.lastname: Hany
authors.forename: M
authors.initials: M
authors.lastname: Dickinson
authors.forename: N
authors.initials: N
authors.lastname: Boonham
mesh_terms: D016000:Cluster Analysis; D016679:Genome, Viral; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017860:Nepovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D011198:Solanum tuberosum / Q000821:virology; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins