title: SGOL1 variant B induces abnormal mitosis and resistance to taxane in non-small cell lung cancers.
abstract: Mitosis is the most conspicuous cell cycle phase and Shugoshin-like 1 (SGOL1) is a key protein in protecting sister chromatids from precocious separation during mitosis. We studied the role of SGOL1 and its splice variants in non-small cell lung cancer (NSCLC) using 82 frozen NSCLC tissue samples. SGOL1-B expression was prevalent in smokers, in cases with a wild-type (WT) EGFR status, and in cases with the focal copy number amplification of genes that are known to be important for defining the biological behaviors of NSCLC. The overexpression of SGOL1-B1 in an NSCLC cell line induced aberrant chromosome missegregation, precociously separated chromatids, and delayed mitotic progression. A higher level of SGOL1-B mRNA was related to taxane resistance, while the forced downregulation of SGOL1-B increased the sensitivity to taxane. These results suggest that the expression of SGOL1-B causes abnormal mitosis and taxane resistance in NSCLC cells.
authors.affiliation: 1] Department of Tumor Pathology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan [2] Second Division, Department of Internal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Matsuura
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Kahyo
authors.forename: Kazuya
authors.initials: K
authors.lastname: Shinmura
authors.forename: Moriya
authors.initials: M
authors.lastname: Iwaizumi
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Yamada
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Funai
authors.forename: Jun
authors.initials: J
authors.lastname: Kobayashi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Tanahashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Niwa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ogawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Takahashi
authors.forename: Naoki
authors.initials: N
authors.lastname: Inui
authors.forename: Takafumi
authors.initials: T
authors.lastname: Suda
authors.forename: Kingo
authors.initials: K
authors.lastname: Chida
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Sugimura
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D017398:Alternative Splicing; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001952:Bridged-Ring Compounds / Q000494:pharmacology*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D018797:Cell Cycle Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D005260:Female; D005784:Gene Amplification; D018628:Gene Dosage; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D014644:Genetic Variation; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008938:Mitosis; D009367:Neoplasm Staging; D012307:Risk Factors; D043823:Taxoids / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D001952:Bridged-Ring Compounds; D018797:Cell Cycle Proteins; C496644:SGO1 protein, human; D043823:Taxoids; C080625:taxane; D066246:ErbB Receptors