title: Investigations of TB vaccine-induced mucosal protection in mice.
abstract: A better understanding of mucosal immunity is required to develop more protective vaccines against Mycobacterium tuberculosis. We developed a murine aerosol challenge model to investigate responses capable of protecting against mucosal infection. Mice received vaccinations intranasally with CpG-adjuvanted antigen 85B (Ag85B/CpG) and/or Bacillus Calmette-Guerin (BCG). Protection against aerosol challenge with a recombinant GFP-expressing BCG was assessed. Mucosal prime/boost vaccinations with Ag85B/CpG and BCG were protective, but did not prevent lung infection indicating more efficacious mucosal vaccines are needed. Our novel finding that protection correlated with increased airway dendritic cells early post-challenge could help guide the development of enhanced mucosal vaccines.
authors.affiliation: Department of Internal Medicine, Saint Louis University, Doisy Research Center, 8th Floor, 1100 S. Grand Blvd., St. Louis, MO 63104, United States.
authors.forename: Azra
authors.initials: A
authors.lastname: Blazevic
authors.affiliation: Department of Internal Medicine, Saint Louis University, Doisy Research Center, 8th Floor, 1100 S. Grand Blvd., St. Louis, MO 63104, United States.
authors.forename: Christopher S
authors.initials: CS
authors.lastname: Eickhoff
authors.affiliation: Department of Internal Medicine, Saint Louis University, Doisy Research Center, 8th Floor, 1100 S. Grand Blvd., St. Louis, MO 63104, United States.
authors.forename: Jaime
authors.initials: J
authors.lastname: Stanley
authors.affiliation: Department of Molecular Microbiology and Immunology, Saint Louis University, Doisy Research Center, 7th Floor, 1100 S. Grand Blvd., St. Louis, MO 63104, United States.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Buller
authors.affiliation: Department of Molecular Microbiology and Immunology, Saint Louis University, Doisy Research Center, 7th Floor, 1100 S. Grand Blvd., St. Louis, MO 63104, United States.
authors.forename: Jill
authors.initials: J
authors.lastname: Schriewer
authors.affiliation: Department of Molecular Microbiology and Immunology, Saint Louis University, Doisy Research Center, 7th Floor, 1100 S. Grand Blvd., St. Louis, MO 63104, United States.
authors.forename: Eric M
authors.initials: EM
authors.lastname: Kettelson
authors.affiliation: Department of Internal Medicine, Saint Louis University, Doisy Research Center, 8th Floor, 1100 S. Grand Blvd., St. Louis, MO 63104, United States; Department of Molecular Microbiology and Immunology, Saint Louis University, Doisy Research Center, 7th Floor, 1100 S. Grand Blvd., St. Louis, MO 63104, United States. Electronic address: hoftdf@slu.edu.
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Hoft
mesh_terms: D000818:Animals; D001500:BCG Vaccine / Q000008:administration & dosage / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D004195:Disease Models, Animal; D018928:Immunity, Mucosal; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D020545:Respiratory Mucosa / Q000276:immunology / Q000382:microbiology; D014376:Tuberculosis / Q000517:prevention & control*; D032581:Tuberculosis Vaccines / Q000276:immunology*
chemical_list: D001500:BCG Vaccine; D032581:Tuberculosis Vaccines
keywords: BCG; Mucosal specific immunity; Mycobacterium tuberculosis (Mtb); Vaccination