title: Determination of HPV prevalence in oral/oropharyngeal mucosa samples in a rural district of São Paulo.
abstract: Knowledge about HPV infection in the oral cavity/oropharynx may contribute to the elucidation of the role it plays in Head and Neck Squamous Cell Carcinoma (HNSCC).
  
  
  OBJECTIVE
  To determine the effectiveness of the methodology used for sampling the oral cavity and oropharynx mucosae and to determine the prevalence of HPV in the oral cavity and oropharynx of adults and children.
  
  
  METHODS
  The study population was served by an assistance program in a rural district of São Paulo. The subjects were asked to donate samples regardless of complaints.
  
  
  CONCLUSIONS
  The study included 47 men, 77 women and 22 children, of which the oral cavity samples were obtained by gargling with commercially-available antiseptic mouthwash. We found 3 positive samples (2.4%) in adults: 2 HPV 55 and one HPV 58. No positive results were found in children. Furthermore we concluded that the sampling method with the mouthwash proved effective and fast for the detection of HPV in the oral cavity and oropharynx in the general population.
authors.affiliation: Faculdade de Ciências Médicas, Santa Casa de São Paulo.
authors.forename: Vitor Breseghello
authors.initials: VB
authors.lastname: Cavenaghi
authors.forename: Elias Jean Eid
authors.initials: EJ
authors.lastname: Ghosn
authors.forename: Natália
authors.initials: N
authors.lastname: Cruz
authors.forename: Lia Mara
authors.initials: LM
authors.lastname: Rossi
authors.forename: Leonardo
authors.initials: L
authors.lastname: da Silva
authors.forename: Henrique Olival
authors.initials: HO
authors.lastname: Costa
authors.forename: Luísa Lina
authors.initials: LL
authors.lastname: Villa
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000821:virology*; D009960:Oropharynx / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000453:epidemiology*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012424:Rural Population; D012959:Socioeconomic Factors; D055815:Young Adult
chemical_list: D004279:DNA, Viral