title: Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for species identification of Acinetobacter strains isolated from blood cultures.
abstract: The clinical relevance of Acinetobacter species, other than A. baumannii, as human pathogens has not been sufficiently assessed owing to the insufficiency of simple phenotypic clinical diagnostic laboratory tests. Infections caused by these organisms have different impacts on clinical outcome and require different treatment and management approaches. It is therefore important to correctly identify Acinetobacter species. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been introduced to identify a wide range of microorganisms in clinical laboratories, but only a few studies have examined its utility for identifying Acinetobacter species, particularly those of the non-Acinetobacter baumannii complex. We therefore evaluated MALDI-TOF MS for identification of Acinetobacter species by comparing it with sequence analysis of rpoB using 123 isolates of Acinetobacter species from blood. Of the isolates examined, we identified 106/123 (86.2%) to species, and 16/123 (13.0%) could only be identified as acinetobacters. The identity of one isolate could not be established. Of the 106 species identified, 89/106 (84.0%) were confirmed by rpoB sequence analysis, and 17/106 (16.0%) were discordant. These data indicate correct identification of 89/123 (72.4%) isolates. Surprisingly, all blood culture isolates were identified as 13 species of Acinetobacter, and the incidence of Acinetobacter pittii was unexpectedly high (42/123; 34.1%) and exceeded that of A. baumannii (22/123; 17.9%). Although the present identification rate using MALDI-TOF MS is not acceptable for species-level identification of Acinetobacter, further expansion of the database should remedy this situation.
authors.affiliation: Department of Quality Assessment and Control of Medical Device Sterilization, Graduate School of Medicine, University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kishii
authors.forename: K
authors.initials: K
authors.lastname: Kikuchi
authors.forename: N
authors.initials: N
authors.lastname: Matsuda
authors.forename: A
authors.initials: A
authors.lastname: Yoshida
authors.forename: K
authors.initials: K
authors.lastname: Okuzumi
authors.forename: Y
authors.initials: Y
authors.lastname: Uetera
authors.forename: H
authors.initials: H
authors.lastname: Yasuhara
authors.forename: K
authors.initials: K
authors.lastname: Moriya
mesh_terms: D000150:Acinetobacter / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D000151:Acinetobacter Infections / Q000382:microbiology; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods; D006801:Humans; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins
keywords: Acinetobacter; MALDI-TOF MS; blood culture isolates; non-A. baumannii complex; rpo
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