title: Identification and characterization of porcine kobuvirus variant isolated from suckling piglet in Gansu province, China.
abstract: Kobuviruses comprise three species, the Aichivirus A, Aichivirus B, and Aichivirus C (porcine kobuvirus). Porcine kobuvirus is endemic to pig farms and is not restricted geographically but, rather, is distributed worldwide. The complete genomic sequences of four porcine kobuvirus strains isolated during a diarrhea outbreak in piglets in the Gansu province of China were determined. Two of these strains exhibited variations relative to the traditional strains. The potential 3C/3D cleavage sites of the variant strains were Q/C, which differed from the Q/S in the traditional porcine kobuvirus genome. A 90-nucleotide deletion in the 2B protein and a single nucleotide insertion in the 3'UTR were found in the variant strains. The VP1 regions of all four porcine kobuviruses in our study were highly variable (81%-86%). Ten common amino acid mutations were found specifically at certain positions within the VP1 region. Significant recombination sites were identified using SimPlot scans of whole genome sequences. Porcine kobuviruses were also detected in pig serum, indicating that the virus can escape the gastrointestinal tract and travel to the circulatory system. These findings suggest that mutations and recombination events may have contributed to the high level of genetic diversity of porcine kobuviruses and serve as a driving force in its evolution.
authors.affiliation: Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China. gaoyuwei@gmail.com.
authors.forename: Shengtao
authors.initials: S
authors.lastname: Fan
authors.forename: Heting
authors.initials: H
authors.lastname: Sun
authors.forename: Ying
authors.initials: Y
authors.lastname: Ying
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Gao
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Yicong
authors.initials: Y
authors.lastname: Yu
authors.forename: Yuanguo
authors.initials: Y
authors.lastname: Li
authors.forename: Tiecheng
authors.initials: T
authors.lastname: Wang
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Yu
authors.forename: Songtao
authors.initials: S
authors.lastname: Yang
authors.forename: Yongkun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Gao
authors.forename: Xianzhu
authors.initials: X
authors.lastname: Xia
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001769:Blood / Q000821:virology; D002681:China; D016000:Cluster Analysis; D003967:Diarrhea / Q000662:veterinary / Q000821:virology; D005243:Feces / Q000821:virology; D014644:Genetic Variation; D016679:Genome, Viral; D052699:Kobuvirus / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000662:veterinary* / Q000821:virology; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013552:Swine; D013553:Swine Diseases / Q000821:virology*
chemical_list: D012367:RNA, Viral