title: A fluorogenic probe for γ-glutamyl cyclotransferase: application of an enzyme-triggered O-to-N acyl migration-type reaction.
abstract: Light it up: human chromosome 7 ORF 24, a tumor-related protein, has been identified as a γ-glutamyl cyclotransferase (GGCT) in the glutathione homeostasis cycle. The singular substrate preference of the enzyme has hampered chemical probe development, and no fluorogenic probe has been reported. Here we report the first fluorogenic dipeptide probe, LISA-4, which should contribute toward further understanding of GGCT.
authors.affiliation: SAITO Research Center, Peptide Institute, Inc. 7-2-9 Saito-Asagi, Ibaraki, Osaka 567-0085 (Japan). t.yoshiya@peptide.co.jp.
authors.forename: Taku
authors.initials: T
authors.lastname: Yoshiya
authors.forename: Shugo
authors.initials: S
authors.lastname: Tsuda
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Mochizuki
authors.forename: Koushi
authors.initials: K
authors.lastname: Hidaka
authors.forename: Yuko
authors.initials: Y
authors.lastname: Tsuda
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kiso
authors.forename: Susumu
authors.initials: S
authors.lastname: Kageyama
authors.forename: Hiromi
authors.initials: H
authors.lastname: Ii
authors.forename: Tatsuhiro
authors.initials: T
authors.lastname: Yoshiki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nishiuchi
mesh_terms: D001665:Binding Sites; D055162:Biocatalysis; D002897:Chromosomes, Human, Pair 7; D004151:Dipeptides / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D062105:Molecular Docking Simulation; D009584:Nitrogen / Q000737:chemistry / Q000378:metabolism*; D016366:Open Reading Frames; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D005722:gamma-Glutamylcyclotransferase / Q000235:genetics / Q000378:metabolism*
chemical_list: D004151:Dipeptides; D005456:Fluorescent Dyes; D005722:gamma-Glutamylcyclotransferase; D009584:Nitrogen; D010100:Oxygen
keywords: O-to-N acyl migration; activity-based probes; cancer; fluorescent probes; gamma-glutamyl cyclotransferase