title: Parvalbumin is overexpressed in the late phase of pharmacological preconditioning in skeletal muscle.
abstract: Pharmacological preconditioning (PPC) with mitochondrial ATP-sensitive K(+) channel openers such as diazoxide, provides protection against ischemia in cardiac muscle, skeletal muscle, and other tissues. Effects on Ca(2+) homeostasis during the late phase of PPC have been described in cardiomyocytes, but no information is available regarding intracellular Ca(2+) changes in skeletal muscle fibers during late PPC. Intracellular Ca(2+) signals were measured in single fibers of adult mouse skeletal muscle, with fluorescent probes, 48 h after the administration of diazoxide. Parvalbumin levels in the myofibers were quantitated by Western blot. Diazoxide induction of late PPC was confirmed by partial protection of muscles from peroxide-induced damage. Late PPC was associated with a significant decrease in the duration of Ca(2+) signals during single twitches and tetanus with no changes in peak values. This effect was prevented by the reactive oxygen species (ROS) scavenger tiron. Late PPC was accompanied by a 30% increase in parvalbumin levels, and this effect was also blocked by tiron. Our data show, for the first time, a role of parvalbumin in late PPC in skeletal muscle.
authors.affiliation: Departamento de Farmacología. Centro de Investigación y de Estudios Avanzados del I.P.N., México, D.F. 07360.
authors.forename: Rosario
authors.initials: R
authors.lastname: Solis
authors.forename: Elba D
authors.initials: ED
authors.lastname: Carrillo
authors.forename: Ascención
authors.initials: A
authors.lastname: Hernández
authors.forename: María C
authors.initials: MC
authors.lastname: García
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Sánchez
mesh_terms: D000465:Algorithms; D000814:Aniline Compounds; D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000037:antagonists & inhibitors / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D003981:Diazoxide / Q000494:pharmacology; D004558:Electric Stimulation; D005456:Fluorescent Dyes; D006706:Homeostasis / Q000187:drug effects; D054086:KATP Channels / Q000819:agonists*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018485:Muscle Fibers, Skeletal / Q000187:drug effects; D018482:Muscle, Skeletal / Q000187:drug effects*; D010320:Parvalbumins / Q000096:biosynthesis*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D017382:Reactive Oxygen Species; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism; D014966:Xanthenes
chemical_list: D000814:Aniline Compounds; D005456:Fluorescent Dyes; D054086:KATP Channels; D010320:Parvalbumins; D047428:Protein Kinase Inhibitors; D017382:Reactive Oxygen Species; D014966:Xanthenes; C059715:Fluo-3; D017868:Cyclic AMP-Dependent Protein Kinases; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D003981:Diazoxide; D002118:Calcium