title: U87MG glioma cells overexpressing IL-17 acclerate early-stage growthin vivoand cause a higher level of CD31 mRNA expression in tumor tissues.
abstract: Immunological alterations have been reported to be involved in glioma, the most common malignant disease of the adult brain. Our recent study identified higher levels of IL-17 in glioma specimens. The present study investigated the role and possible mechanisms of IL-17 in glioma tumorigenesis. Human IL-17 cDNA was cloned and inserted into the eukaryotic pEGFP-N1 expression vector, which was used to transfect the glioma U87MG cell line, resulting in a high level of IL-17 expression in these cells. The cells were then transfected with IL-17 (pEGFP-N1-IL-17-U87MG) or mock (pEGFP-N1-U87MG) vector or left untransfected (U87MG) and subcutaneously inoculated into the right flank of nude mice. The results revealed that the pEGFP-N1-IL-17-U87MG cells grew more rapidly in the early stages (P<0.05, determined on day 32 post-inoculation compared with the other two groups). Quantitative (q)PCR detected higher mouse (m)CD31 mRNA levels in the IL-17-transfected group (P<0.01) compared with the mock-transfected and untransfected groups. IL-17 transfection altered the mRNA expression of a panel of molecules that are associated with immunity and inflammation in U87MG cellsin vitro. An effect of the vector was identified, whereby the mock transfection strongly inhibited cell growthin vivoand dramatically altered the mRNA levels of multiple molecules in the cell culturein vitrocompared with the untransfected cells. The present study confirmed that IL-17 overexpression may enhance glioma cell growthin vivo, which may be associated with accelerated angiogenesis. IL-17 overexpression may also alter the cellular mRNA expression of immune-related molecules.
authors.affiliation: Department of Lab Medicine, Gongli Hospital, Second Military Medicine University, Pudong New Area, Shanghai 200135, P.R. China ; Department of Lab Medicine, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Hu
authors.forename: Hongxing
authors.initials: H
authors.lastname: Ye
authors.forename: Denghai
authors.initials: D
authors.lastname: Zhang
authors.forename: Weiwei
authors.initials: W
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Mao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lu
keywords: IL-17; glioma; nude mice; pEGFP-N1; tumorigenesis