title: A rapid and sensitive LC-ESI-MS/MS method for the detection of YF-49-92.MLS in rat plasma.
abstract: BACKGROUND
  YF-49-92.MLS is a novel candidate for TB treatment. An accurate, precise and specific LC-MS/MS method for the quantification of YF-49-92.MLS in rat plasma using verapamil as an IS is reported in this paper.
  
  
  METHODS
  Proper retention time and excellent peak shape were acquired using an Agilent Zorbax(®) SB-C18 column with the mobile phase of 5 mmol/l ammonium acetate, 0.1% formic acid-methanol (30:70, v/v). The LLOQ was 1 ng/ml. The calibration curves encompassed concentrations from 20 to 5000 ng/ml. Intra- and inter-assay precision and accuracy were within 15% by determining low, medium and high concentration samples. Extraction recovery, stability, and matrix effects were also fully validated.
  
  
  CONCLUSIONS
  This method has been validated to be rapid and sensitive, and successfully applied to the PK study of YF-49-92.MLS in rat plasma.
authors.affiliation: Jiangsu Center for Drug Screening, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Gao
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.forename: Zhenzhou
authors.initials: Z
authors.lastname: Jiang
authors.forename: Guanlan
authors.initials: G
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Xiao
authors.forename: Lixin
authors.initials: L
authors.lastname: Sun
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Luyong
authors.initials: L
authors.lastname: Zhang
authors.forename: Shaofei
authors.initials: S
authors.lastname: Xie
authors.forename: Haiyan
authors.initials: H
authors.lastname: Huang
mesh_terms: D000085:Acetates; D000818:Animals; D000995:Antitubercular Agents / Q000097:blood*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods* / Q000592:standards; D005260:Female; D005561:Formates; D007093:Imidazoles / Q000097:blood*; D008297:Male; D000432:Methanol; D010078:Oxazines / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods* / Q000592:standards; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards; D014700:Verapamil / Q000097:blood
chemical_list: D000085:Acetates; D000995:Antitubercular Agents; D005561:Formates; D007093:Imidazoles; D010078:Oxazines; C588407:YF-49-92 compound; C030544:formic acid; D014700:Verapamil; C018824:ammonium acetate; D000432:Methanol