title: Gene expression profile study on osteoinductive effect of natural hydroxyapatite.
abstract: The aim of this study was to investigate the osteoinductive effect of natural hydroxyapatite (NHA). NHA was extracted from pig bones and prepared into disk-like samples. Then, proliferation of mouse bone mesenchymal stem cells (MSCs) cultured on NHA was assessed by the methylthiazoltetrazolium (MTT) assay. Furthermore, microarray technology was applied to obtain the gene expression profiles of MSCs cultured on NHA at 24, 48, and 72 h. The gene expression profile was then comprehensively analyzed by clustering, Gene Ontology (GO), Gene Microarray Pathway Profiler (GenMAPP) and Ingenuity Pathway Analysis (IPA). According to the results of microarray experiment, 8992 differentially expressed genes were obtained. 90 differential expressed genes related to HA osteogenic differentiation were determined by GO analysis. These genes included not only 6 genes related to HA osteogenic differentiation as mentioned in the literatures but also newly discovered 84 genes. Some important signaling pathways (TGF-β, MAPK, Wnt, etc.) were influenced by these genes. Gene interaction networks were obtained by IPA software, in which the scoring values of two networks were highest, and their main functions were related to cell development. The comprehensive analysis of these results indicate that NHA regulate some crucial genes (e.g., Bmp2, Spp1) and then activate some pathways such as TGF-β signaling pathway, and ultimately osteogenic differentiation was induced.
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University, Nanjing, Jiangsu, 210096, People's Republic of China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Lü
authors.forename: Jiandan
authors.initials: J
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Zhang
authors.forename: Lifeng
authors.initials: L
authors.lastname: Zhao
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D016000:Cluster Analysis; D019295:Computational Biology; D017886:Durapatite / Q000494:pharmacology*; D020869:Gene Expression Profiling; D063990:Gene Ontology; D053263:Gene Regulatory Networks / Q000187:drug effects; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D016348:Osseointegration / Q000187:drug effects* / Q000235:genetics*; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics; D011208:Powders; D060888:Real-Time Polymerase Chain Reaction; D012015:Reference Standards; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D017550:Spectroscopy, Fourier Transform Infrared; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D011208:Powders; D016212:Transforming Growth Factor beta; D017886:Durapatite
keywords: bioinformatics; gene expression profile; mesenchymal stem cells (MSCs); natural hydroxyapatite (NHA); osteoinduction