title: Integrin-mediated osteoblastic adhesion on a porous manganese-incorporated TiO2coating prepared by plasma electrolytic oxidation.
abstract: This study was conducted to evaluate the bioactivity of manganese-incorporated TiO2(Mn-TiO2) coating prepared on titanium (Ti) plate by plasma electrolytic oxidation (PEO) technique in Ca-, P- and Mn-containing electrolytes. The surface topography, phase and element compositions of the coatings were investigated using scanning electron microscopy (SEM), X-ray diffraction (XRD) and energy dispersive spectrometry (EDS), respectively. The adhesion of osteoblast-like MG63 cells onto Ti, TiO2and Mn-TiO2surfaces was evaluated, and the signal transduction pathway involved was confirmed by the sequential expression of the genes for integrins β1, β3, α1and α3, focal adhesion kinase (FAK), and the extracellular regulated kinases (ERKs), including ERK1 and ERK2. The results obtained indicated that Mn was successfully incorporated into the porous nanostructured TiO2coating, and did not alter the surface topography or the phase composition of the coating. The adhesion of the MG63 cells onto the Mn-incorporated TiO2coating was significantly enhanced compared with that on the Mn-free TiO2coating and the pure Ti plates. In addition, the enhanced cell adhesion on the Mn-TiO2coatings may have been mediated by the binding of the integrin subunits, β1and α1, and the subsequent signal transduction pathway, involving FAK and ERK2. The study indicated that the novel Mn-TiO2coating has potential for orthopedic implant applications, and that further investigations are required.
authors.affiliation: Orthopedic Department, The Affiliated Taizhou People's Hospital of Nantong University, Taizhou, Jiangsu 225300, P.R. China.
authors.forename: Zhenxiang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Beibei
authors.initials: B
authors.lastname: Gu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.forename: Lixian
authors.initials: L
authors.lastname: Zhu
keywords: MG63; TiO2; adhesion; manganese; plasma electrolytic oxidation