title: Differential proteomic profiling in human spermatozoa that did or did not result in pregnancy via IVF and AID.
abstract: OBJECTIVE
  To identify biochemical markers in men with idiopathic infertility and normal sperm counts.
  
  
  METHODS
  We obtained proteomic profiling proteins in human spermatozoa following successful or unsuccessful pregnancy via assisted reproductive technology (ART) using 6-plex tandem mass tag (TMT) isobaric mass spectrometry. Our study design consisted of two groups: 1. The semen of 6 men whose sperm resulted in a clinical pregnancy following ART and 6 men whose semen did not result in a clinical pregnancy following ART. The results of differentiated mass spectrometry were validated by Western blotting.
  
  
  CONCLUSIONS
  A total of 2,045 proteins were detected in our cohort. 21 proteins were found to be differentially expressed (>1.2-fold) in men whose sperm resulted in a clinical pregnancy and those that did not. Using the results of bioinformatics analysis and Western Blotting, three proteins (A2LD1, ATP1B3 and FBXO2) were shown to have the same differential pattern (p<0.05) that was observed in the mass spectrometry analysis.
  
  
  CONCLUSIONS
  Proteomics may help identity a select cohort of men with abnormal semen parameters and aide infertility diagnoses.
authors.affiliation: Renji Hospital, Department of Urology, Sperm Development and Genetics Laboratory, Shanghai Human Sperm Bank, Shanghai Institute of Andrology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200001, P. R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhu
authors.forename: Yibo
authors.initials: Y
authors.lastname: Wu
authors.forename: Ke
authors.initials: K
authors.lastname: Jin
authors.forename: Hui
authors.initials: H
authors.lastname: Lu
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Yueshuai
authors.initials: Y
authors.lastname: Guo
authors.forename: Feng
authors.initials: F
authors.lastname: Yan
authors.forename: Wenbo
authors.initials: W
authors.lastname: Shi
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Cao
authors.forename: Hongliang
authors.initials: H
authors.lastname: Hu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhu
authors.forename: Xuejiang
authors.initials: X
authors.lastname: Guo
authors.forename: Jiahao
authors.initials: J
authors.lastname: Sha
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.forename: Zuoming
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D018797:Cell Cycle Proteins / Q000032:analysis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D044783:F-Box Proteins / Q000032:analysis / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007316:Insemination, Artificial, Heterologous; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism; D011247:Pregnancy; D040901:Proteomics; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis / Q000378:metabolism; D013094:Spermatozoa / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D005722:gamma-Glutamylcyclotransferase / Q000032:analysis / Q000378:metabolism
chemical_list: C519304:ATP1B3 protein, human; D018797:Cell Cycle Proteins; D044783:F-Box Proteins; C485508:FBXO2 protein, human; D009419:Nerve Tissue Proteins; D005722:gamma-Glutamylcyclotransferase; D000254:Sodium-Potassium-Exchanging ATPase
keywords: AID; Biomarker; IVF; Infertility; Proteomics