title: Genetic characteristics of the human hepatic stellate cell line LX-2.
abstract: The human hepatic cell line LX-2 has been described as tool to study mechanisms of hepatic fibrogenesis and the testing of antifibrotic compounds. It was originally generated by immortalisation with the Simian Vacuolating Virus 40 (SV40) transforming (T) antigen and subsequent propagation in low serum conditions. Although this immortalized line is used in an increasing number of studies, detailed genetic characterisation has been lacking. We here have performed genetic characterisation of the LX-2 cell line and established a single-locus short tandem repeat (STR) profile for the cell line and characterized the LX-2 karyotype by several cytogenetic and molecular cytogenetic techniques. Spectral karyotyping (SKY) revealed a complex karyotype with a set of aberrations consistently present in the metaphases analyses which might serve as cytogenetic markers. In addition, various subclonal and single cell aberrations were detected. Our study provides criteria for genetic authentication of LX-2 and offers insights into the genotype changes which might underlie part of its phenotypic features.
authors.affiliation: Institute of Clinical Chemistry and Pathobiochemistry, RWTH Aachen University, Aachen, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Weiskirchen
authors.forename: Jörg
authors.initials: J
authors.lastname: Weimer
authors.forename: Steffen K
authors.initials: SK
authors.lastname: Meurer
authors.forename: Anja
authors.initials: A
authors.lastname: Kron
authors.forename: Barbara
authors.initials: B
authors.lastname: Seipel
authors.forename: Inga
authors.initials: I
authors.lastname: Vater
authors.forename: Norbert
authors.initials: N
authors.lastname: Arnold
authors.forename: Reiner
authors.initials: R
authors.lastname: Siebert
authors.forename: Lieming
authors.initials: L
authors.lastname: Xu
authors.forename: Scott L
authors.initials: SL
authors.lastname: Friedman
authors.forename: Carsten
authors.initials: C
authors.lastname: Bergmann
mesh_terms: D002460:Cell Line; D055166:Hepatic Stellate Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D059785:Karyotype; D007621:Karyotyping