title: Preparation of mesoporous silica embedded pipette tips for rapid enrichment of endogenous peptides.
abstract: Mesoporous silica embedded pipette tips (mSiO2-Tips) were successfully prepared by a simple method and applied to rapid enrichment of endogenous peptides for the first time. The prepared mSiO2-Tips showed low back pressure when solvent was pipetted up and down. As a result, mSiO2-Tip could selectively trap peptides and exclude high-MW proteins simultaneously based on size-exclusion mechanism due to the uniform mesopore structure of the sorbent bed. The in-pipette-tip SPE approach was proved to be easy-operation, sensitive and fast (less than 2 min) for the analysis of peptides, which was further successfully applied in the efficient enrichment of peptides from human plasma.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
authors.forename: Gang-Tian
authors.initials: GT
authors.lastname: Zhu
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: He
authors.forename: Xiao-Shui
authors.initials: XS
authors.lastname: Li
authors.forename: Shao-Ting
authors.initials: ST
authors.lastname: Wang
authors.forename: Yan-Bo
authors.initials: YB
authors.lastname: Luo
authors.forename: Bi-Feng
authors.initials: BF
authors.lastname: Yuan
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Feng
mesh_terms: D000097:Acetonitriles; D006801:Humans; D010455:Peptides / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000097:Acetonitriles; D010455:Peptides; D012822:Silicon Dioxide; C032159:acetonitrile
keywords: MALDI-TOF mass spectrometry; Miniaturized SPE; Peptide; Pipette tip