title: Role of pncA and rpsA gene sequencing in detection of pyrazinamide resistance in Mycobacterium tuberculosis isolates from southern China.
abstract: We sequenced pncA and rpsA genes plus flanking regions of 161 Mycobacterium tuberculosis isolates and found 10 new pncA and 3 novel rpsA mutations in pyrazinamide-resistant strains determined by the Bactec MGIT 960 system. The 3' end of rpsA might be added as the target of molecular detection of pyrazinamide susceptibility.
authors.affiliation: State Key Laboratory of Respiratory Diseases, Department of Clinical Laboratory, Guangzhou Chest Hospital, Guangzhou, China.
authors.forename: Yaoju
authors.initials: Y
authors.lastname: Tan
authors.forename: Zuqiong
authors.initials: Z
authors.lastname: Hu
authors.forename: Tianyu
authors.initials: T
authors.lastname: Zhang
authors.forename: Xingshan
authors.initials: X
authors.lastname: Cai
authors.forename: Haobin
authors.initials: H
authors.lastname: Kuang
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Liu
authors.forename: Junyu
authors.initials: J
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Yang
authors.forename: Ke
authors.initials: K
authors.lastname: Zhang
authors.forename: Shouyong
authors.initials: S
authors.lastname: Tan
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000581:Amidohydrolases / Q000235:genetics*; D000995:Antitubercular Agents / Q000494:pharmacology*; D002681:China; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D020125:Mutation, Missense; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics*; D011718:Pyrazinamide / Q000494:pharmacology*; D012269:Ribosomal Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D000995:Antitubercular Agents; D012269:Ribosomal Proteins; C015494:ribosomal protein S1; D011718:Pyrazinamide; D000581:Amidohydrolases; C487496:PncA protein, Mycobacterium tuberculosis