title: Smart "lanthano" proteins for phospholipid sensing.
abstract: Metal-ion-mediated interactions between calcium-binding peripheral proteins and membrane phospholipids is a key feature of multiple cell signaling processes. The molecular basis for the interaction involves the displacement of inner-sphere water molecules on calcium ions by phosphate groups of the phospholipids. On the basis of this fundamental mechanism, we have devised a novel "turn-on" optical sensing strategy for anionic phospholipids by using a lanthanide reconstituted protein. The "lanthano" protein turns on selectively in the presence of a crucial signaling phospholipid, phosphatidylserine, by affording a 6 times enhancement in lanthanide luminescence. The "turn-on" sensing strategy was distinctly validated by direct evidence for the water-displacement mechanism via lifetime measurements.
authors.affiliation: Department of Chemical Sciences, Tata Institute of Fundamental Research , 1 Homi Bhabha Road, Colaba, Mumbai 400005, India.
authors.forename: Shafali
authors.initials: S
authors.lastname: Gupta
authors.forename: Samsuzzoha
authors.initials: S
authors.lastname: Mondal
authors.forename: Amit
authors.initials: A
authors.lastname: Mhamane
authors.forename: Ankona
authors.initials: A
authors.lastname: Datta
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000838:Anions; D017304:Annexin A5 / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D010743:Phospholipids / Q000032:analysis* / Q000378:metabolism; D014867:Water
chemical_list: D000838:Anions; D017304:Annexin A5; D028581:Lanthanoid Series Elements; D010743:Phospholipids; D014867:Water; D000255:Adenosine Triphosphate