title: Quantitative proteomic analysis of intact plastids.
abstract: Plastids are specialized cell organelles in plant cells that are differentiated into various forms including chloroplasts, chromoplasts, and amyloplasts, and fulfill important functions in maintaining the overall cell metabolism and sensing environmental factors such as sunlight. It is therefore important to grasp the mechanisms of differentiation and functional changes of plastids in order to enhance the understanding of vegetality. In this chapter, details of a method for the extraction of intact plastids that makes analysis possible while maintaining the plastid functions are provided; in addition, a quantitative shotgun method for analyzing the composition and changes in the content of proteins in plastids as a result of environmental impacts is described.
authors.affiliation: Niigata Agricultural Research Institute, Nagaoka, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shiraya
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kaneko
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Mitsui
mesh_terms: D000595:Amino Acid Sequence; D002499:Centrifugation, Density Gradient; D005374:Filtration; D007475:Ion Exchange Resins; D007553:Isotope Labeling; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D012275:Oryza / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification; D018087:Plastids / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D036226:Seedlings / Q000378:metabolism; D013482:Superoxide Dismutase / Q000737:chemistry
chemical_list: D007475:Ion Exchange Resins; D010940:Plant Proteins; D013482:Superoxide Dismutase