title: Laser-capture dissection and immunohistochemistry reveals chloride and mucous-cell specific gene expression in gills of seawater acclimated Atlantic salmon Salmo salar.
abstract: Laser-capture microdissection and immunohistochemistry were used to show that gene and protein expression varied in different cell types in the gills of Atlantic salmon Salmo salar, with chloride cells found to express high levels of sodium potassium ATPase and mucous cells expressing elevated levels of anterior gradient protein. It is therefore important that studies of gene expression in gill tissue take account of the proportion of the various cell types present.
authors.affiliation: National Centre for Marine Conservation and Resource Sustainability, University of Tasmania, Locked Bag 1370, Launceston, 7250, Tasmania, Australia.
authors.forename: B
authors.initials: B
authors.lastname: Nowak
authors.forename: K
authors.initials: K
authors.lastname: Cadoret
authors.forename: S W
authors.initials: SW
authors.lastname: Feist
authors.forename: T P
authors.initials: TP
authors.lastname: Bean
mesh_terms: D000064:Acclimatization; D000818:Animals; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005880:Gills / Q000166:cytology* / Q000378:metabolism; D007150:Immunohistochemistry; D061346:Laser Capture Microdissection; D009088:Mucoproteins / Q000235:genetics / Q000378:metabolism; D020317:Salmo salar / Q000235:genetics* / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism
chemical_list: D029941:Fish Proteins; D009088:Mucoproteins; D000254:Sodium-Potassium-Exchanging ATPase
keywords: anadromous; anterior gradient protein; microdissection; qPCR; salmonid