title: Interference of ferric ions with ferrous iron quantification using the ferrozine assay.
abstract: The ferrozine assay is a widely used colorimetric method for determining soluble iron concentrations. We provide evidence for a heretofore unrecognized interference of ferric ions (Fe(3+)) on ferrous iron (Fe(2+)) measurements performed in the dark. Fe(3+) concentrations affected the absorbance measurements, which linearly increased with incubation time.
authors.affiliation: Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA; Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN 37996, USA.
authors.forename: Jeongdae
authors.initials: J
authors.lastname: Im
authors.forename: Jaejin
authors.initials: J
authors.lastname: Lee
authors.forename: Frank E
authors.initials: FE
authors.lastname: Löffler
mesh_terms: D002623:Chemistry Techniques, Analytical; D003124:Colorimetry / Q000379:methods*; D003624:Darkness; D003951:Diagnostic Errors; D005290:Ferric Compounds / Q000378:metabolism*; D005296:Ferrous Compounds / Q000032:analysis*; D005297:Ferrozine / Q000378:metabolism*; D007477:Ions / Q000032:analysis; D007501:Iron / Q000032:analysis*; D013997:Time Factors
chemical_list: D005290:Ferric Compounds; D005296:Ferrous Compounds; D007477:Ions; D005297:Ferrozine; D007501:Iron
keywords: Ferric; Ferrous; Ferrozine; Interference