title: A solid state fungal fermentation-based strategy for the hydrolysis of wheat straw.
abstract: This paper reports a solid-state fungal fermentation-based pre-treatment strategy to convert wheat straw into a fermentable hydrolysate. Aspergillus niger was firstly cultured on wheat straw for production of cellulolytic enzymes and then the wheat straw was hydrolyzed by the enzyme solution into a fermentable hydrolysate. The optimum moisture content and three wheat straw modification methods were explored to improve cellulase production. At a moisture content of 89.5%, 10.2 ± 0.13 U/g cellulase activity was obtained using dilute acid modified wheat straw. The addition of yeast extract (0.5% w/v) and minerals significantly improved the cellulase production, to 24.0 ± 1.76 U/g. The hydrolysis of the fermented wheat straw using the fungal culture filtrate or commercial cellulase Ctec2 was performed, resulting in 4.34 and 3.13 g/L glucose respectively. It indicated that the fungal filtrate harvested from the fungal fermentation of wheat straw contained a more suitable enzyme mixture than the commercial cellulase.
authors.affiliation: School of Biosciences, Sutton Bonington Campus, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom.
authors.forename: Nattha
authors.initials: N
authors.lastname: Pensupa
authors.forename: Meng
authors.initials: M
authors.lastname: Jin
authors.forename: Matt
authors.initials: M
authors.lastname: Kokolski
authors.forename: David B
authors.initials: DB
authors.lastname: Archer
authors.forename: Chenyu
authors.initials: C
authors.lastname: Du
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000096:biosynthesis; D005285:Fermentation; D005944:Glucosamine / Q000378:metabolism; D006813:Humidity; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism; D013997:Time Factors; D014908:Triticum / Q000737:chemistry*; D014866:Waste Products / Q000032:analysis*
chemical_list: D014866:Waste Products; C036909:lignocellulose; D008031:Lignin; D002480:Cellulase; D005944:Glucosamine
keywords: Aspergillus niger; Cellulase; Hydrolysis; Solid state fermentation (SSF); Wheat straw