title: Three-dimensional collagen scaffold enhances the human adenoid cystic carcinoma cancer stem cell and epithelial-mesenchymal transition properties.
abstract: Three-dimensional (3D) cell culture may provide the architectures similar to the in vivo natural extracellular matrix condition for in vitro cultured cells. In this work, a 3D collagen scaffold was used to culture the adenoid cystic carcinoma (ACC) cells. The results showed that the 3D scaffold not only induced the diversification of cell morphologies but also increased the cell proliferation. The transcription of matrix metalloproteinase and epithelial-mesenchymal transition were significantly increased in the cells cultured in 3D collagen scaffolds. In addition, the expression of cancer stem cell (CSC) markers of Sox2 and Oct4 were higher than that in 2D cultured cells. The 3D cultured ACC-83 cells showed more resistance to chemotherapeutic drugs. Thus, the 3D collagen scaffold could provide a useful model for CSCs study and anticancer therapeutics research in vitro.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi'an, 710032, China; Department of Oral and Maxillofacial Surgery, School of Stomatology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, 830054, China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhi-Feng
authors.initials: ZF
authors.lastname: Xiao
authors.forename: Bin
authors.initials: B
authors.lastname: Dai
authors.forename: Mo-Yi
authors.initials: MY
authors.lastname: Sun
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
mesh_terms: D042501:Angiogenic Proteins / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D001761:Bleomycin / Q000031:analogs & derivatives / Q000494:pharmacology; D003528:Carcinoma, Adenoid Cystic / Q000473:pathology*; D018929:Cell Culture Techniques; D002455:Cell Division; D045744:Cell Line, Tumor; D002465:Cell Movement; D002945:Cisplatin / Q000494:pharmacology; D003094:Collagen; D019008:Drug Resistance, Neoplasm; D004354:Drug Screening Assays, Antitumor; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020782:Matrix Metalloproteinases / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D009368:Neoplasm Transplantation; D014411:Neoplastic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000637:transplantation; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054457:Tissue Scaffolds; D014750:Vincristine / Q000494:pharmacology
chemical_list: D042501:Angiogenic Proteins; D009363:Neoplasm Proteins; D001761:Bleomycin; C025703:bleomycetin; D014750:Vincristine; D003094:Collagen; D020782:Matrix Metalloproteinases; D002945:Cisplatin
keywords: adenoid cystic carcinoma; cancer stem cell; collagen scaffold; epithelial; mesenchymal transition; three-dimensional culture