title: Real-time label-free surface plasmon resonance biosensing with gold nanohole arrays fabricated by nanoimprint lithography.
abstract: In this work we present a surface plasmon resonance sensor based on enhanced optical transmission through sub-wavelength nanohole arrays. This technique is extremely sensitive to changes in the refractive index of the surrounding medium which result in a modulation of the transmitted light. The periodic gold nanohole array sensors were fabricated by high-throughput thermal nanoimprint lithography. Square periodic arrays with sub-wavelength hole diameters were obtained and characterized. Using solutions with known refractive index, the array sensitivities were obtained. Finally, protein absorption was monitored in real-time demonstrating the label-free biosensing capabilities of the fabricated devices.
authors.affiliation: CIC microGUNE, Arrasate-Mondragón 20500, Spain. jmartinez@cicmicrogune.es.
authors.forename: Josu
authors.initials: J
authors.lastname: Martinez-Perdiguero
authors.forename: Aritz
authors.initials: A
authors.lastname: Retolaza
authors.forename: Deitze
authors.initials: D
authors.lastname: Otaduy
authors.forename: Aritz
authors.initials: A
authors.lastname: Juarros
authors.forename: Santos
authors.initials: S
authors.lastname: Merino
mesh_terms: D000042:Absorption; D015374:Biosensing Techniques / Q000295:instrumentation*; D003199:Computer Systems; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D054802:Molecular Imprinting / Q000379:methods; D058608:Nanopores / Q000648:ultrastructure*; D036103:Nanotechnology / Q000295:instrumentation*; D010316:Particle Size; D040081:Protein Array Analysis / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling
chemical_list: D006046:Gold