title: Modified Jiu Wei Qiang Huo decoction improves dysfunctional metabolomics in influenza A pneumonia-infected mice.
abstract: In order to study the effective mechanism of a traditional Chinese medicine (TCM), modified Jiu Wei Qiang Huo decoction (MJWQH), against H1N1-induced pneumonia in mice, we chose a holistic approach. A reverse-phase liquid chromatography with quadruple time-of-flight mass spectrometry (LC-Q-TOF-MS) was developed to determine metabolomic biomarkers in mouse serum for the MJWQH effects. Thirteen biomarkers of H1N1-induced pneumonia in mice serum were identified, which comprised l-valine, lauroylcarnitine, palmitoyl-l-carnitine, l-ornithine, uric acid, taurine, O-succinyl-l-homoserine, l-leucine, l-phenylalanine, PGF2α, 20-ethyl-PGE2, arachidonic acid, and glycerophospho-N-arachidonoyl ethanolamine. Among them, metabolites of amino acids, fatty acids and arachidonic acid had the most relevant changes in mice with H1N1-induced pneumonia. MJWQH effectively improved weight loss, lung index, biomarkers and inflammatory mediators such as prostaglandin E2 and phospholipase A2 in the infected mice. Importantly, MJWQH reversed the elevated biomarkers to the control levels from the infection, which provided a systematic view and a theoretical basis for its prevention or treatment. The results suggest that the protective effect of MJWQH against H1N1-induced pneumonia is possibly through regulation of pathways for amino acid, fatty acid and arachidonic acid metabolism. They also suggest that the LC-MS-based metabolomic strategy is a powerful tool for elucidation of the mechanisms of TCM.
authors.affiliation: Department of Chinese Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200092, People's Republic of China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Chen
authors.forename: Jiajun
authors.initials: J
authors.lastname: Fan
authors.forename: Yubin
authors.initials: Y
authors.lastname: Li
authors.forename: Xunlong
authors.initials: X
authors.lastname: Shi
authors.forename: Dianwen
authors.initials: D
authors.lastname: Ju
authors.forename: Qianlin
authors.initials: Q
authors.lastname: Yan
authors.forename: Xin
authors.initials: X
authors.lastname: Yan
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D053118:Influenza A Virus, H1N1 Subtype; D008297:Male; D055442:Metabolome / Q000187:drug effects*; D051379:Mice; D008813:Mice, Inbred ICR; D009976:Orthomyxoviridae Infections / Q000188:drug therapy / Q000378:metabolism*; D011024:Pneumonia, Viral / Q000188:drug therapy / Q000378:metabolism*; D015203:Reproducibility of Results
chemical_list: D000998:Antiviral Agents; D015415:Biomarkers; D004365:Drugs, Chinese Herbal
keywords: TCM; antiviral; biomarkers; influenza virus; metabolomics; pneumonia