title: A quantitative analysis of the polyamine in lung cancer patient fingernails by LC-ESI-MS/MS.
abstract: A quantitative analysis of polyamines in lung cancer patient fingernails by the combination of 4-(N,N-dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole derivatives and liquid chromatography-electrospray ionization tandem mass spectrometry is described. The reaction of the reagent with eight kinds of polyamines, that is, N(1) -acetylputrescine (N(1) -actPUT), N(8) -acetylspermidine, N(1) -acetylspermine, 1,3-diaminopropane, putrescine (PUT), cadaverine, spermidine and spermine (SPM) effectively occurs at 60 °C for 30 min. The detection limits (signal-to-noise ratio 5) were 5-100 fmol. A good linearity was achieved from the calibration curves, which was obtained by plotting the peak area ratios of the analytes relative to the internal standard (IS), that is, 1,6-diaminohexane, vs the injected amounts of polyamines (r(2)  > 0.996), and the intra-day and inter-day assay precisions were <9.84%. Furthermore, the recoveries (%) of the polyamines spiked in the human fingernails were 89.14-110.64. The present method was applied to human fingernail samples from 17 lung cancer patients and 39 healthy volunteers. The polyamine concentration was different based on the gender, that is, the N(1) -actPUT and PUT contents were 3.10 times and 2.56 times higher in healthy men than in women, respectively. Additionally, in the lung cancer patient group, as compared with the healthy volunteers, the concentrations of SPM had a statistically significant (p < 0.05) correlation. Therefore, because the proposed method provides a good mass accuracy and the trace detection of the polyamines in human fingernails, this analytical technique could be a noninvasive technique to assist in the diagnosis and assessment of disease activity in lung cancer patients.
authors.affiliation: Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan; College of Pharmacy, Yanbian University, Yanji City, 133002, Jilin Province, People's Republic of China.
authors.forename: Jun Zhe
authors.initials: JZ
authors.lastname: Min
authors.forename: Akihito
authors.initials: A
authors.lastname: Matsumoto
authors.forename: Gao
authors.initials: G
authors.lastname: Li
authors.forename: Ying-Zi
authors.initials: YZ
authors.lastname: Jiang
authors.forename: Hai-Fu
authors.initials: HF
authors.lastname: Yu
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Todoroki
authors.forename: Koichi
authors.initials: K
authors.lastname: Inoue
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Toyo'oka
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008175:Lung Neoplasms / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009262:Nails / Q000737:chemistry* / Q000378:metabolism; D011073:Polyamines / Q000032:analysis* / Q000378:metabolism; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D055815:Young Adult
chemical_list: D011073:Polyamines
keywords: LC-ESI-MS/MS; human fingernail; lung cancer; polyamine