title: Antibiotic resistance of Shewanella putrefaciens isolated from shellfish collected from the West Sea in Korea.
abstract: In this study, we isolated and characterized Shewanella putrefaciens from shellfish harvested from the West Sea in Korea. For the initial isolation of S. putrefaciens, LB agar plates supplemented with ferrous sulfate and sodium thiosulfate were inoculated with shellfish homogenates, incubated for 24h, and then black colonies were selected. Gram-negative and catalase-positive colonies were subsequently confirmed by PCR assays and API 20E kit test system. The Shewanella-specific 16S rRNA and gyrB genes were used to design S. putrefaciens-specific PCR primers. From 6 species of shellfish tested, 24 S. putrefaciens strains were isolated. These 24 isolates had the following profiles of resistance against 16 antibiotics: all the isolates were resistant to cephalothin and vancomycin and 95.8% were resistant to ampicillin. Here, we report the isolation of S. putrefaciens from shellfish and our results point to a new possible route for exposing healthy individuals to S. putrefaciens.
authors.affiliation: Department of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon 402-751, Republic of Korea.
authors.forename: Chang-Ho
authors.initials: CH
authors.lastname: Kang
authors.forename: YuJin
authors.initials: Y
authors.lastname: Shin
authors.forename: HanEul
authors.initials: H
authors.lastname: Jeon
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Choi
authors.forename: SuYeon
authors.initials: S
authors.lastname: Jeong
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: So
mesh_terms: D000818:Animals; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004784:Environmental Monitoring; D008974:Mollusca / Q000382:microbiology*; D009792:Oceans and Seas; D056910:Republic of Korea; D012758:Shellfish / Q000382:microbiology*; D020614:Shewanella putrefaciens / Q000235:genetics* / Q000302:isolation & purification; D014876:Water Pollution / Q000032:analysis / Q000706:statistics & numerical data
keywords: Antibiotic resistance; Shellfish; Shewanella putrefaciens; gyrB