title: Enhancement of root hydraulic conductivity by methyl jasmonate and the role of calcium and abscisic acid in this process.
abstract: The role of jasmonic acid in the induction of stomatal closure is well known. However, its role in regulating root hydraulic conductivity (L) has not yet been explored. The objectives of the present research were to evaluate how JA regulates L and how calcium and abscisic acid (ABA) could be involved in such regulation. We found that exogenous methyl jasmonate (MeJA) increased L of Phaseolus vulgaris, Solanum lycopersicum and Arabidopsis thaliana roots. Tomato plants defective in JA biosynthesis had lower values of L than wild-type plants, and that L was restored by addition of MeJA. The increase of L by MeJA was accompanied by an increase of the phosphorylation state of the aquaporin PIP2. We observed that MeJA addition increased the concentration of cytosolic calcium and that calcium channel blockers inhibited the rise of L caused by MeJA. Treatment with fluoridone, an inhibitor of ABA biosynthesis, partially inhibited the increase of L caused by MeJA, and tomato plants defective in ABA biosynthesis increased their L after application of MeJA. It is concluded that JA enhances L and that this enhancement is linked to calcium and ABA dependent and independent signalling pathways.
authors.affiliation: Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008, Granada, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Sánchez-Romera
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Ruiz-Lozano
authors.forename: Guowei
authors.initials: G
authors.lastname: Li
authors.forename: Doan-Trung
authors.initials: DT
authors.lastname: Luu
authors.forename: Maria del Carmen
authors.initials: Mdel C
authors.lastname: Martínez-Ballesta
authors.forename: Micaela
authors.initials: M
authors.lastname: Carvajal
authors.forename: Angel María
authors.initials: AM
authors.lastname: Zamarreño
authors.forename: Jose María
authors.initials: JM
authors.lastname: García-Mina
authors.forename: Christophe
authors.initials: C
authors.lastname: Maurel
authors.forename: Ricardo
authors.initials: R
authors.lastname: Aroca
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism* / Q000494:pharmacology; D000085:Acetates / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects / Q000502:physiology*; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002614:Chelating Agents / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology; D007811:Lanthanum / Q000494:pharmacology; D018551:Solanum lycopersicum / Q000187:drug effects / Q000502:physiology*; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000494:pharmacology*; D027805:Phaseolus / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D013194:Staining and Labeling; D014867:Water
chemical_list: D000085:Acetates; D002121:Calcium Channel Blockers; D002614:Chelating Agents; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C028521:lanthanum chloride; D014867:Water; D007811:Lanthanum; D000040:Abscisic Acid; D006493:Heparin; C072239:methyl jasmonate; D002118:Calcium
keywords: Arabidopsis thaliana; Phaseolus vulgaris; Solanum lycopersicum; aquaporins