title: CD177 expression on neutrophils: in search of a clonal assay for myeloid neoplasia by flow cytometry.
abstract: OBJECTIVE
  To determine whether the fraction of CD177+ neutrophils might be altered in clonal myeloid disorders, similar to the skewed κ/λ ratio for B-cell lymphomas, and could be used to identify myeloid neoplasms.
  
  
  METHODS
  Blood and bone marrow samples were evaluated for the fraction of CD177+ neutrophils by flow cytometry.
  
  
  RESULTS
  Skewed high neutrophil CD177(%) was not associated with neoplasia, but skewed low neutrophil CD177(%) was highly correlated with clonal myeloid disorders at values less than 40%. Specificity of low neutrophil CD177(%) for clonal myeloid disorders was 87% with a 40% cutoff and 95% with a 30% cutoff. Findings were most pronounced for myelodysplasia, with 52% (11/21) containing fewer than 40% CD177+ neutrophils. Specificity was also suggested by normalization of neutrophil CD177(%) in four patients who reached morphologic remission after therapy for myelodysplasia or acute leukemia.
  
  
  CONCLUSIONS
  Skewed low neutrophil CD177(%) is highly associated with clonal myeloid disorders, particularly myelodysplasia, and may be useful for detecting clonal myeloid disorders.
authors.affiliation: Dept of Pathology, Room 528A RB&C, University Hospitals Case Medical Center, 11100 Euclid Ave, Cleveland, OH 44106; e-mail: hjm2@case.edu.
authors.forename: Howard J
authors.initials: HJ
authors.lastname: Meyerson
authors.forename: Ebeneezer
authors.initials: E
authors.lastname: Osei
authors.forename: Karen
authors.initials: K
authors.lastname: Schweitzer
authors.forename: Georgetta
authors.initials: G
authors.lastname: Blidaru
authors.forename: Alison
authors.initials: A
authors.lastname: Edinger
authors.forename: Anna
authors.initials: A
authors.lastname: Balog
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001681:Biological Assay; D014408:Biomarkers, Tumor / Q000378:metabolism; D001854:Bone Marrow Cells / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D002999:Clone Cells / Q000473:pathology; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D058851:GPI-Linked Proteins / Q000378:metabolism; D006801:Humans; D007223:Infant; D007519:Isoantigens / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000175:diagnosis* / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism / Q000473:pathology*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C407242:CD177 protein, human; D058851:GPI-Linked Proteins; D007519:Isoantigens; D011956:Receptors, Cell Surface
keywords: CD177; Flow cytometry; Myelodysplasia; Neutrophil