title: A photoactivatable drug-caged fluorophore conjugate allows direct quantification of intracellular drug transport.
abstract: We report here a method that utilizes a photoactivatable drug-caged fluorophore conjugate to quantify intracellular drug trafficking processes at single cell resolution. Photoactivation is performed in labeled cellular compartments to visualize intracellular drug exchange under physiological conditions, without the need for washing, facilitating its translation into in vivo cancer models.
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St., Boston, MA 02114 (USA).
authors.forename: Sarit S
authors.initials: SS
authors.lastname: Agasti
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St., Boston, MA 02114 (USA).
authors.forename: Ashley M
authors.initials: AM
authors.lastname: Laughney
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St., Boston, MA 02114 (USA).
authors.forename: Rainer H
authors.initials: RH
authors.lastname: Kohler
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St., Boston, MA 02114 (USA).|Department of Systems Biology, Harvard Medical School, 200 Longwood Ave., Alpert 536, Boston, MA 02115 (USA).
authors.forename: Ralph
authors.initials: R
authors.lastname: Weissleder
mesh_terms: D001692:Biological Transport; D001896:Boron Compounds / Q000737:chemistry; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism*; D055668:Photochemical Processes; D010793:Phthalazines / Q000737:chemistry / Q000378:metabolism; D010879:Piperazines / Q000737:chemistry / Q000378:metabolism
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D004337:Drug Carriers; D005456:Fluorescent Dyes; D010793:Phthalazines; D010879:Piperazines; C531550:olaparib