title: Elevation of seizure threshold after intranasal administration of anti-glutamate antibodies to C57BL/6 mice.
abstract: The dose-dependent effect of intranasal administration of antibodies to glutamate on acute generalized epileptic activity was studied in C57Bl/6 mice. It was shown that pretreatment with antibodies in doses of 100, 300, and 500 μg/kg 1 h before titration with convulsant pentylenetetrazole had anticonvulsant effects. This treatment was followed by an increase in the thresholds of clonic and tonic seizures with fatal outcome and lengthening of seizure latency. The most pronounced effect was observed in doses of 300 and 500 μg/kg. Comparison of the anticonvulsant effect of antibodies in active immunization with glutamate-BSA conjugate and systemic intraperitoneal administration of purified anti-glutamate antibodies revealed advantages of intranasal administration.
authors.affiliation: Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia. karpova777@inbox.ru.
authors.forename: M N
authors.initials: MN
authors.lastname: Karpova
authors.forename: L V
authors.initials: LV
authors.lastname: Kuznetsova
authors.forename: L A
authors.initials: LA
authors.lastname: Vetrile
authors.forename: N J
authors.initials: NJ
authors.lastname: Klishina
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D000927:Anticonvulsants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D018698:Glutamic Acid / Q000276:immunology*; D007274:Injections, Intraperitoneal; D051379:Mice; D008810:Mice, Inbred C57BL; D010433:Pentylenetetrazole; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000517:prevention & control*
chemical_list: D000906:Antibodies; D000927:Anticonvulsants; D018698:Glutamic Acid; D010433:Pentylenetetrazole