title: Simultaneous determination of 14 oil-soluble synthetic dyes in chilli products by high performance liquid chromatography with a gel permeation chromatography clean-up procedure.
abstract: A method was developed for the simultaneous determination of 14 fat-soluble dyes in chilli products. The samples were extracted with hexane/acetone. The cleanup was performed with gel permeation chromatography (GPC) cleanup system. A HPLC separation was performed using variable wavelength detector and a gradient elution with 0.1% formic acid and methanol-acetonitrile (1:1, v/v) as the mobile phases. Good linearity (R² ≥ 0.995) was observed between 0.1 and 5.0 μg/mL. Detection limits of the investigated dyes, which were evaluated at signal to noise ratio of 3, were in the ranges of 11-71 μg/kg. The recoveries of the 14 synthetic colourants in three matrices ranged from 73.4% to 103.5%. Relative standard deviations ranged from 3.7% to 12.3%. The method has been successfully used for the determination of banned dyes in real samples.
authors.affiliation: Center for Quality Supervision & Inspection of Food, Chongqing Academy of Metrology and Quality Inspection, Chongqing Engineering Research Center for Food Safety, Chongqing 401123, China. Electronic address: zyh218@yahoo.com.cn.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Zhu
authors.forename: Bo
authors.initials: B
authors.lastname: Zhao
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Xiao
authors.forename: Wen
authors.initials: W
authors.lastname: Yun
authors.forename: Zhaojing
authors.initials: Z
authors.lastname: Xiao
authors.forename: Dawei
authors.initials: D
authors.lastname: Tu
authors.forename: Shiqi
authors.initials: S
authors.lastname: Chen
mesh_terms: D053000:Analytic Sample Preparation Methods; D002138:Calibration; D002212:Capsicum / Q000737:chemistry* / Q000378:metabolism; D002681:China; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003212:Condiments / Q000032:analysis; D005505:Food Coloring Agents / Q000032:analysis* / Q000737:chemistry; D005506:Food Contamination; D005511:Food Handling; D005513:Food Inspection / Q000379:methods; D057141:Food, Preserved / Q000032:analysis; D005638:Fruit / Q000737:chemistry* / Q000378:metabolism; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction; D010938:Plant Oils / Q000737:chemistry*; D015203:Reproducibility of Results; D012995:Solubility; D013053:Spectrophotometry; D017365:Spices / Q000032:analysis
chemical_list: D005505:Food Coloring Agents; D010938:Plant Oils
keywords: Chilli products; Gel permeation chromatography; High performance liquid chromatography; Synthetic dyes