title: Immunoproteomics analysis of food allergens.
abstract: Immunoblot-coupled proteomics based on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), namely, immunoproteomics, has been used for comprehensive identification of food allergens, because it is a simple and inexpensive tool for rapid identification of several IgE-binding proteins. In this section, we describe our protocols for identification of food allergens using immunoproteomics and discuss a few technical points in detail.
authors.affiliation: National Institute of Health Sciences (NIHS), Setagaya-ku, Tokyo, Japan.
authors.forename: Rika
authors.initials: R
authors.lastname: Nakamura
authors.forename: Reiko
authors.initials: R
authors.lastname: Teshima
mesh_terms: D000485:Allergens / Q000276:immunology*; D005453:Fluorescence; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007525:Isoelectric Focusing; D013058:Mass Spectrometry; D012275:Oryza / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000276:immunology*; D040901:Proteomics / Q000379:methods*
chemical_list: D000485:Allergens; D010940:Plant Proteins