title: Correlation between the EGF gene intronic polymorphism, rs2298979, and colorectal cancer.
abstract: Colorectal cancer (CRC) is an important disorder that results from genetic and epigenetic alterations in one colonic epithelial cell. Epidermal growth factor (EGF) is critical in the development of tumors in epithelial tissues. Variations in the DNA sequence of theEGFgene may be particularly significant with regard to susceptibility to CRC. The present study aimed to investigate the effect of theEGFgene single nucleotide polymorphism (SNP), rs2298979, on CRC. In this prospective study, 220 samples were collected from patients with CRC and compared with 220 matched healthy controls. Genotyping was performed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, and the result was validated by direct sequencing. A significant correlation was observed between the rs2298979 variant in theEGFgene and CRC. The frequency of the A/G genotype in the control group was higher than in the patients with sporadic CRC [odds ratio (OR), 0.488; 95% confidence interval (CI), 0.307-0.774; P=0.002]. In this study there were no individuals with a G/G genotype. Although the frequency of the G and A alleles was similar in the healthy control and CRC patient groups, individuals with the A/G genotype were less susceptible to CRC compared with those with the A/A genotype.
authors.affiliation: Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Chaleshi
authors.forename: Mahdi Montazer
authors.initials: MM
authors.lastname: Haghighi
authors.forename: Sanaz
authors.initials: S
authors.lastname: Savabkar
authors.forename: Neda
authors.initials: N
authors.lastname: Zali
authors.forename: Mohsen
authors.initials: M
authors.lastname: Vahedi
authors.forename: Mahsa
authors.initials: M
authors.lastname: Khanyaghma
authors.forename: Gholam Reza
authors.initials: GR
authors.lastname: Javadi
authors.forename: Hamid
authors.initials: H
authors.lastname: Asadzade
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Zali
keywords: colorectal cancer; epidermal growth factor; rs2298979; single nucleotide polymorphism