title: Effects of diet-induced mild obesity on airway hyperreactivity and lung inflammation in mice.
abstract: OBJECTIVE
  Obesity has been suggested to be linked to asthma. However, it is not yet known whether obesity directly leads to airway hyperreactivity (AHR) or obesity-induced airway inflammation associated with asthma. We investigated obesity- related changes in adipokines, AHR, and lung inflammation in a murine model of asthma and obesity.
  
  
  METHODS
  We developed mouse models of chronic asthma via ovalbumin (OVA)-challenge and of obesity by feeding a high-fat diet, and then performed the methacholine bronchial provocation test, and real-time PCR for leptin, leptin receptor, adiponectin, adiponectin receptor (adipor1 and 2), vascular endothelial growth factor (VEGF), transforming growth factor (TGF) β, and tumor necrosis factor (TNF) α in lung tissue. We also measured cell counts in bronchoalveolar lavage fluid.
  
  
  RESULTS
  Both obese and lean mice chronically exposed to OVA developed eosinophilic lung inflammation and AHR to methacholine. However, obese mice without OVA challenge did not develop AHR or eosinophilic inflammation in lung tissue. In obese mice, lung mRNA expressions of leptin, leptin receptor, VEGF, TGF, and TNF were enhanced, and adipor1 and 2 expressions were decreased compared to mice in the control group. On the other hand, there were no differences between obese mice with or without OVA challenge.
  
  
  CONCLUSIONS
  Diet-induced mild obesity may not augment AHR or eosinophilic lung inflammation in asthma.
authors.affiliation: Department of Pediatrics, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, 29 Saemunan-ro, Jongno-gu, Seoul 110-746, Korea. jy7.shim@samsung.com.
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Jung
authors.forename: Jang-Mi
authors.initials: JM
authors.lastname: Kwon
authors.forename: Jae Won
authors.initials: JW
authors.lastname: Shim
authors.forename: Deok Soo
authors.initials: DS
authors.lastname: Kim
authors.forename: Hye Lim
authors.initials: HL
authors.lastname: Jung
authors.forename: Moon Soo
authors.initials: MS
authors.lastname: Park
authors.forename: Soo-Hee
authors.initials: SH
authors.lastname: Park
authors.forename: Jinmi
authors.initials: J
authors.lastname: Lee
authors.forename: Won-Young
authors.initials: WY
authors.lastname: Lee
authors.forename: Jung Yeon
authors.initials: JY
authors.lastname: Shim
mesh_terms: D000818:Animals; D001249:Asthma / Q000503:physiopathology; D016535:Bronchial Hyperreactivity / Q000503:physiopathology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D004041:Dietary Fats / Q000009:adverse effects; D051379:Mice; D009765:Obesity / Q000209:etiology* / Q000503:physiopathology*; D011014:Pneumonia / Q000503:physiopathology*; D015291:Transforming Growth Factors / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; D015291:Transforming Growth Factors
keywords: Adipokine; airway hyperresponsiveness; asthma; high fat; obesity; transforming growth factor beta; tumor necrosis factor alpha; vascular endothelial growth factor