title: Immobilization of 5-fluorouridine on chitosan.
abstract: The 2',3'-O-levulinic acid derivative 2b of the cancerostatic 5-fluorouridine as well as its N(3)-farnesylated nucleolipid 2d were synthesized and coupled to H2 O-soluble chitosanes of different molecular weight and at various pH values (3.5-5.5) leading to 6 and 7. In addition, the coumarine fluorophore ATTO-488 N(9)-butanoate was bound to the biopolymer by a sequential-coupling technique to afford 9 and 10. Moreover, chitosan foils were prepared, to which 2b was coupled. Their degradation by chitosanase (from Streptomyces sp. N174) was studied UV-spectrophotometrically in a Franz diffusion cell.
authors.affiliation: Organic Materials Chemistry and Bioorganic Chemistry, Institute of Chemistry of New Materials, University of Osnabrück, Barbarastrasse 7, D-49069 Osnabrück.
authors.forename: Edith
authors.initials: E
authors.lastname: Malecki
authors.forename: Rebecca
authors.initials: R
authors.lastname: Viere
authors.forename: Helmut
authors.initials: H
authors.lastname: Rosemeyer
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007982:Levulinic Acids / Q000737:chemistry; D054876:Prenylation; D013056:Spectrophotometry, Ultraviolet; D014529:Uridine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D007982:Levulinic Acids; C001943:5-fluorouridine; D048271:Chitosan; C032246:levulinic acid; D014529:Uridine
keywords: Chitosane; Immobilized drugs; Lipophilization; Uridine, 5-fluoro-