title: Nocardiosis in Mediterranean bivalves: first detection of Nocardia crassostreae in a new host Mytilus galloprovincialis and in Ostrea edulis from the Gulf of Naples (Italy).
abstract: In this work M. galloprovincialis and O. edulis specimens were surveyed for a pathological study in the Gulf of Naples (Mediterranean sea, Campania Region, southern Italy). Clusters of Nocardia sp.-like cells were observed in histological slides. PCR amplification, sequencing and in situ hybridization were carried out in order to corroborate Nocardia species identification for both hosts. Blast results showed a 99% of maximum identity with Nocardia crassostreae sequences in Genbank. This is the first report of N. crassostreae in the new host M. galloprovincialis and, in a new area, the Mediterranean Sea.
authors.affiliation: Department of Biological Sciences, University of Naples Federico II, 80134 Napoli, Italy. Electronic address: francesca.carella@unina.it.
authors.forename: Francesca
authors.initials: F
authors.lastname: Carella
authors.forename: Noelia
authors.initials: N
authors.lastname: Carrasco
authors.forename: Karl B
authors.initials: KB
authors.lastname: Andree
authors.forename: Beatriz
authors.initials: B
authors.lastname: Lacuesta
authors.forename: Dolors
authors.initials: D
authors.lastname: Furones
authors.forename: Gionata
authors.initials: G
authors.lastname: De Vico
mesh_terms: D000818:Animals; D054884:Host-Pathogen Interactions; D007558:Italy; D008522:Mediterranean Sea; D008969:Molecular Sequence Data; D049878:Mytilus / Q000382:microbiology*; D009615:Nocardia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D049895:Ostrea / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
keywords: Aquaculture; Gulf of Naples; Mytilus galloprovincialis; Nocardia; Nocardia crassotreae; Ostrea edulis