title: A high-throughput three-dimensional cell migration assay for toxicity screening with mobile device-based macroscopic image analysis.
abstract: There is a growing demand for in vitro assays for toxicity screening in three-dimensional (3D) environments. In this study, 3D cell culture using magnetic levitation was used to create an assay in which cells were patterned into 3D rings that close over time. The rate of closure was determined from time-lapse images taken with a mobile device and related to drug concentration. Rings of human embryonic kidney cells (HEK293) and tracheal smooth muscle cells (SMCs) were tested with ibuprofen and sodium dodecyl sulfate (SDS). Ring closure correlated with the viability and migration of cells in two dimensions (2D). Images taken using a mobile device were similar in analysis to images taken with a microscope. Ring closure may serve as a promising label-free and quantitative assay for high-throughput in vivo toxicity in 3D cultures.
authors.affiliation: 1] Department of Physics, Rice University, Houston, TX 77005 USA [2] Nano3D Biosciences (n3D), Houston, TX 77030 USA [3].
authors.forename: David M
authors.initials: DM
authors.lastname: Timm
authors.forename: Jianbo
authors.initials: J
authors.lastname: Chen
authors.forename: David
authors.initials: D
authors.lastname: Sing
authors.forename: Jacob A
authors.initials: JA
authors.lastname: Gage
authors.forename: William L
authors.initials: WL
authors.lastname: Haisler
authors.forename: Shane K
authors.initials: SK
authors.lastname: Neeley
authors.forename: Robert M
authors.initials: RM
authors.lastname: Raphael
authors.forename: Mehdi
authors.initials: M
authors.lastname: Dehghani
authors.forename: Kevin P
authors.initials: KP
authors.lastname: Rosenblatt
authors.forename: T C
authors.initials: TC
authors.lastname: Killian
authors.forename: Hubert
authors.initials: H
authors.lastname: Tseng
authors.forename: Glauco R
authors.initials: GR
authors.lastname: Souza
mesh_terms: D018929:Cell Culture Techniques; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D057166:High-Throughput Screening Assays / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D007052:Ibuprofen / Q000633:toxicity; D020128:Inhibitory Concentration 50; D008853:Microscopy / Q000379:methods; D014116:Toxicology
chemical_list: D007052:Ibuprofen