title: Spatially controlled photochemical peptide and polymer conjugation on biosurfaces.
abstract: An efficient phototriggered Diels-Alder conjugation is utilized to graft in an effective and straightforward approach poly(trifluoro ethyl methacrylate) (Mn = 3700 Da, Đ = 1.27) and a model peptide (GIGKFLHS) onto thin hyaluronan films and cellulose surfaces. The surfaces were functionalized with an o-quinodimethane moiety - capable of releasing a caged diene - via carbodiimide mediated coupling. The o-quinodimethane group is employed as a photoactive linker to tether predefined peptide/polymer strands in a spatially controlled manner onto the biosurface by photoenol ligation. An in-depth characterization employing XPS, ToF-SIMS, SPR, ellipsometry, and AFM was conducted to evidence the effectiveness of the presented approach.
authors.affiliation: Preparative Macromolecular Chemistry, Institut für Technische Chemie und Polymerchemie, Karlsruhe Institute of Technology (KIT) , Engesserstr. 18, 76128 Karlsruhe, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Tischer
authors.forename: Tanja K
authors.initials: TK
authors.lastname: Claus
authors.forename: Michael
authors.initials: M
authors.lastname: Bruns
authors.forename: Vanessa
authors.initials: V
authors.lastname: Trouillet
authors.forename: Katharina
authors.initials: K
authors.lastname: Linkert
authors.forename: Cesar
authors.initials: C
authors.lastname: Rodriguez-Emmenegger
authors.forename: Anja S
authors.initials: AS
authors.lastname: Goldmann
authors.forename: Sébastien
authors.initials: S
authors.lastname: Perrier
authors.forename: Hans G
authors.initials: HG
authors.lastname: Börner
authors.forename: Christopher
authors.initials: C
authors.lastname: Barner-Kowollik
mesh_terms: D000595:Amino Acid Sequence; D002482:Cellulose / Q000031:analogs & derivatives* / Q000138:chemical synthesis*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D003432:Cross-Linking Reagents / Q000737:chemistry; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D018625:Microscopy, Atomic Force; D010446:Peptide Fragments / Q000737:chemistry*; D055668:Photochemical Processes; D011109:Polymethacrylic Acids / Q000138:chemical synthesis*; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D023822:Tissue Engineering
chemical_list: D020099:Coated Materials, Biocompatible; D003432:Cross-Linking Reagents; D010446:Peptide Fragments; D011109:Polymethacrylic Acids; D002482:Cellulose; D006820:Hyaluronic Acid