title: Molecularly imprinted superparamagnetic iron oxide nanoparticles for rapid enrichment and separation of cholesterol.
abstract: A general protocol to prepare surface molecularly imprinted polymer core-shell superparamagnetic Fe3O4 nanoparticles (Fe3O4@MIP SPNPs), using a surface-mediated RAFT polymerization approach, is described. Cholesterol-imprinted Fe3O4@MIP SPNPs were obtained by oleic acid-stabilized Fe3O4 nanoparticles with a trithiocarbonate agent and subsequently by polymerizing thin molecularly imprinted layers composed of dimethylacrylamide and N,N'-methylene(bis)acrylamide units. The surface-mediated RAFT polymerization approach allows the synthesis of ∼20 nm hybrid composite particles with a ∼6 nm MIP shell, exhibiting superparamagnetic properties (saturation magnetization = 35.4 emu g(-1)) and specific molecular recognition of cholesterol. The Fe3O4@MIP SPNPs show the capability of rapid enriching and separating cholesterol (∼3.1% in weight) and are renewable and cyclically exploited due to their monodispersive and superparamagnetic features. Moreover, under optimal conditions, the Fe3O4@MIP SPNP recoveries of spiked human serum, milk, yolk and beef were 91.6%, 93.6%, 92.4% and 91.2%, respectively. Finally, the method of molecular imprinting on superparamagnetic particles can be extended to a wide range of applications for cell sorting, biomolecule enrichment and separation, and drug delivery.
authors.affiliation: Gazi University, Faculty of Science, Department of Chemistry, 06500 Besevler, Ankara, Turkey. caykara@gazi.edu.tr.
authors.forename: Adem
authors.initials: A
authors.lastname: Zengin
authors.forename: Ertan
authors.initials: E
authors.lastname: Yildirim
authors.forename: Ugur
authors.initials: U
authors.lastname: Tamer
authors.forename: Tuncer
authors.initials: T
authors.lastname: Caykara
mesh_terms: D002784:Cholesterol / Q000737:chemistry* / Q000302:isolation & purification; D005290:Ferric Compounds / Q000737:chemistry*; D053768:Metal Nanoparticles; D046529:Microscopy, Electron, Transmission; D054802:Molecular Imprinting
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide; D002784:Cholesterol