title: Deranged epidermal differentiation in kl/kl mouse and the effects of βKlotho siRNA on the differentiation of HaCaT cells.
abstract: Mice deficient in the klotho gene (kl/kl mice) display the phenotypes of human ageing. We found that the expression of epidermal differentiation-associated factors (keratin 1, keratin 10, filaggrin and loricrin) was lower in the skin of kl/kl mice than that of wild-type mice. In vitro experiments showed that the expression of βKlotho, a family of klotho gene-encoded protein, was induced concomitantly with the differentiation of an immortalized human epidermal keratinocyte cell line (HaCaT cells) when they were cultured in an air-liquid interface. βKlotho knockdown by small interfering ribonucleic acid suppressed the expression of the above differentiation-associated factors in HaCaT cells. βKlotho small interfering ribonucleic acid increased the expression of keratin 14, which is expressed in mitotically active basal layer cells, and activated p44/p42 mitogen-activated protein kinase in the HaCaT cells grown in the air-liquid interface. These findings suggest that the epidermal differentiation is deranged in kl/kl mice, and βKlotho is required for the differentiation of human epidermal keratinocytes.
authors.affiliation: Department of Dermatology, Kagawa University, Kita-Gun, Japan.
authors.forename: Kozo
authors.initials: K
authors.lastname: Nakai
authors.forename: Kozo
authors.initials: K
authors.lastname: Yoneda
authors.forename: Reiji
authors.initials: R
authors.lastname: Haba
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kushida
authors.forename: Naomi
authors.initials: N
authors.lastname: Katsuki
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Moriue
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kosaka
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Kubota
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Inoue
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004817:Epidermis / Q000378:metabolism*; D000091344:Filaggrin Proteins; D006801:Humans; D007381:Intermediate Filament Proteins / Q000378:metabolism; D053556:Keratin-1 / Q000378:metabolism; D053550:Keratin-10 / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology; D000090265:Klotho Proteins; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D051379:Mice; D009154:Mutation; D010641:Phenotype; D034741:RNA, Small Interfering / Q000378:metabolism; D012867:Skin / Q000378:metabolism; D015595:Skin Aging
chemical_list: C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; C538995:KLB protein, human; D053556:Keratin-1; C418622:Klb protein, mouse; D008565:Membrane Proteins; D034741:RNA, Small Interfering; C064554:loricrin; D053550:Keratin-10; D000090265:Klotho Proteins
keywords: Klotho; epidermal differentiation