title: The relationship between and clinical significance of MicroRNA-32 and phosphatase and tensin homologue expression in colorectal cancer.
abstract: MicroRNAs (miRNAs, miRs) are suspected to play important roles in carcinogenesis. MiR-32 has altered expression in colorectal cancer (CRC); however, the clinical significance of miR-32 expression in the process of carcinogenesis is poorly understood. In this study, we determined the levels of, the correlation between, and the clinical significance of the expression of miR-32 and phosphatase and tensin homologue (PTEN), a tumor suppressor targeted by miR-32, in CRC. The levels of miR-32 and PTEN gene expression in 35 colorectal carcinoma samples, 35 corresponding cancer-adjacent tissue samples, 27 colorectal adenoma samples, and 16 normal tissue samples were quantified using real-time quantitative reverse transcriptase-polymerase chain reaction. PTEN protein expression was determined using western blot and immunohistochemistry (IHC). The relationship between the miR-32 and PTEN protein expression and clinicopathological factors was analyzed. Significant upregulation of miR-32 expression and reduction of PTEN were identified in CRC tissues. High miR-32 levels were significantly associated with lymph node and distant metastasis, and Kaplan-Meier analysis indicated that patients with high miR-32 expression had a poor overall survival. Low PTEN protein expression was also significantly correlated with distant metastasis. An inverse relationship between miR-32 and PTEN protein expression was identified. In addition, IHC analysis revealed weak or indiscernible PTEN staining in tumor tissue. MiR-32 overexpression was correlated with specific CRC clinicopathological features and may be a marker of poor prognosis in CRC patients. MiR-32 and PTEN expression were inversely correlated, and miR-32 may be associated with the development of CRC.
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College, South Peoples Avenue No. 57, Xiashan District, Zhanjiang, Guangdong, China.
authors.forename: Weiyun
authors.initials: W
authors.lastname: Wu
authors.forename: Pengchun
authors.initials: P
authors.lastname: Yang
authors.forename: Xiao
authors.initials: X
authors.lastname: Feng
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Yumei
authors.initials: Y
authors.lastname: Qiu
authors.forename: Ting
authors.initials: T
authors.lastname: Tian
authors.forename: Yanting
authors.initials: Y
authors.lastname: He
authors.forename: Caiyuan
authors.initials: C
authors.lastname: Yu
authors.forename: Jingfang
authors.initials: J
authors.lastname: Yang
authors.forename: Shicai
authors.initials: S
authors.lastname: Ye
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000236:Adenoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009362:Neoplasm Metastasis; D051059:PTEN Phosphohydrolase / Q000235:genetics* / Q000378:metabolism; D011379:Prognosis; D000070876:Tensins
chemical_list: D014408:Biomarkers, Tumor; C568529:MIRN32 microRNA, human; D035683:MicroRNAs; D008840:Microfilament Proteins; D000070876:Tensins; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human