title: Principal component analysis of odor coding at the level of third-order olfactory neurons in Drosophila.
abstract: Olfactory information in Drosophila is conveyed by projection neurons from olfactory sensory neurons to Kenyon cells (KCs) in the mushroom body (MB). A subset of KCs responds to a given odor molecule, and the combination of these KCs represents a part of the neuronal olfactory code. KCs are also thought to function as coincidence detectors for memory formation, associating odor information with a coincident punishment or reward stimulus. Associative conditioning has been shown to modify KC output. This plasticity occurs in the vertical lobes of MBs containing α/α' branches of KCs, which is shown by measuring the average Ca(2+) levels in the branch of each lobe. We devised a method to quantitatively describe the population activity patterns recorded from axons of >1000 KCs at the α/α' branches using two-photon Ca(2+) imaging. Principal component analysis of the population activity patterns clearly differentiated the responses to distinct odors.
authors.affiliation: Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-0032, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hiroi
authors.forename: Masamichi
authors.initials: M
authors.lastname: Ohkura
authors.forename: Junichi
authors.initials: J
authors.lastname: Nakai
authors.forename: Naoki
authors.initials: N
authors.lastname: Masuda
authors.forename: Koichi
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Kiichi
authors.initials: K
authors.lastname: Inoue
authors.forename: André
authors.initials: A
authors.lastname: Fiala
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tabata
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D003511:Cyclohexanols / Q000494:pharmacology; D004330:Drosophila / Q000502:physiology*; D024521:Mushroom Bodies / Q000166:cytology / Q000502:physiology; D000442:Octanols / Q000494:pharmacology; D009812:Odorants; D018034:Olfactory Receptor Neurons / Q000502:physiology*; D025341:Principal Component Analysis; D012903:Smell
chemical_list: D003511:Cyclohexanols; D000442:Octanols; C035973:4-methylcyclohexanol