title: Recent advances in the development of single cell analysis--a review.
abstract: Development of techniques for the analysis of the content of individual cells represents an important direction in modern bioanalytical chemistry. While the analysis of chromosomes, organelles, or location of selected proteins has been traditionally the domain of microscopic techniques, the advances in miniaturized analytical systems bring new possibilities for separations and detections of molecules inside the individual cells including smaller molecules such as hormones or metabolites. It should be stressed that the field of single cell analysis is very broad, covering advanced optical, electrochemical and mass spectrometry instrumentation, sensor technology and separation techniques. The number of papers published on single cell analysis has reached several hundred in recent years. Thus a complete literature coverage is beyond the limits of a journal article. The following text provides a critical overview of some of the latest developments with the main focus on mass spectrometry, microseparation methods, electrophoresis in capillaries and microfluidic devices and respective detection techniques for performing single cell analyses.
authors.affiliation: Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic. Electronic address: klep@iach.cz.
authors.forename: Karel
authors.initials: K
authors.lastname: Klepárník
authors.forename: František
authors.initials: F
authors.lastname: Foret
mesh_terms: D001419:Bacteria / Q000378:metabolism; D002853:Chromatography, Liquid; D005456:Fluorescent Dyes / Q000737:chemistry; D005658:Fungi / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification; D059010:Single-Cell Analysis / Q000639:trends*
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins
keywords: Capillary electrophoresis; Electrochemistry; Fluorescence; Mass spectrometry; Microfluidic devices; Single cell analysis