title: Extracellular transport of cell-size particles and tumor cells by dendritic cells in culture.
abstract: Many particulate materials of sizes approximating that of a cell disseminate after being introduced into the body. While some move about within phagocytic inflammatory cells, others appear to move about outside of, but in contact with, such cells. In this report, we provide unequivocal photomicroscopic evidence that cultured, mature, human dendritic cells can transport in extracellular fashion over significant distances both polymeric beads and tumor cells. At least in the case of polymeric beads, both fibrinogen and the β2-integrin subunit, CD18, appear to play important roles in the transport process. These discoveries may yield insight into a host of disease-related phenomena, including and especially tumor cell invasion and metastasis.
authors.affiliation: Amnis Corporation, EMD-Millipore, Seattle, WA 98119, United States.
authors.forename: Robert I
authors.initials: RI
authors.lastname: Thacker
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Retzinger
authors.forename: James G
authors.initials: JG
authors.lastname: Cash
authors.forename: Michael D
authors.initials: MD
authors.lastname: Dentler
authors.forename: Gregory S
authors.initials: GS
authors.lastname: Retzinger
mesh_terms: D001692:Biological Transport; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D018821:CD18 Antigens / Q000378:metabolism; D002465:Cell Movement; D048429:Cell Size; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism*; D005260:Female; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D009000:Monocytes / Q000166:cytology* / Q000378:metabolism
chemical_list: D018821:CD18 Antigens; D020099:Coated Materials, Biocompatible; D005340:Fibrinogen
keywords: Cancer; Cell-size particles; Chemotaxis; Dendritic cells; Directed migration; Extracellular transport; Inflammation; Metastasis; Tumor cells