title: Biological behavior of neurally differentiated periodontal ligament stem cells on different titanium implant surfaces.
abstract: We investigated the biological behavior of periodontal ligament stem cells (PDLSCs) induced to differentiate into Schwann cells (SCs) on the surfaces of titanium discs. Two types of titanium surfaces, sand blasted and acid etched (SA) and smooth polished, were prepared on titanium discs, and the behavior of SC-like cells on these discs was investigated. Cell morphology was examined by scanning electron microscopy, and cell proliferation was assessed using assays for methylthiazol tetrazolium metabolism and total protein content. Real-time polymerase chain reaction and Western blotting analyses were used to determine the gene and protein expression, respectively, of SC markers by PDLSCs. Differentiated PDLSCs could adhere, proliferate, differentiate, and express SC marker genes and proteins on the prepared titanium surfaces, and the highest levels of SC marker protein expression were observed in PDLSCs on SA titanium surfaces. SA titanium surfaces show good biocompatibility for the SC-like cells, which is important for the application of these cells in peri-implant nerve tissue engineering, through which they are expected to improve the osseoperception of dental implants.
authors.affiliation: Department of Dentistry, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Li
authors.forename: Dapeng
authors.initials: D
authors.lastname: Liao
authors.forename: Ping
authors.initials: P
authors.lastname: Gong
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.forename: Gang
authors.initials: G
authors.lastname: Sun
mesh_terms: D000134:Acid Etching, Dental; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D004285:Dogs; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000187:drug effects; D020007:Implants, Experimental; D008856:Microscopy, Fluorescence; D010513:Periodontal Ligament / Q000166:cytology*; D012583:Schwann Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*
chemical_list: D014025:Titanium
keywords: Schwann cells; biological behavior; differentiation; periodontal ligament stem cells; titanium surface