title: Chronic myelomonocytic leukemia monocytes uniformly display a population of monocytes with CD11c underexpression.
abstract: OBJECTIVE
  To examine the utility of CD11c expression on monocytes in normal controls and patients with chronic myelomonocytic leukemia (CMML) (n = 23) with flow cytometric immunophenotyping.
  
  
  METHODS
  Twenty-three CMML samples and 10 control bone marrows submitted for lymphoma staging without evidence of disease were examined.
  
  
  RESULTS
  Monocytes in CMML samples ranged from 4% to 35%. Expression of at least one aberrant monocytic marker was found on the monocytes in 18 (82%) of 22 evaluable cases. The most common aberrancy was underexpression of CD11c (n = 15), while none of the bone marrow controls showed underexpression of CD11c.
  
  
  CONCLUSIONS
  A distinct heterogeneous population of monocytic cells with underexpression of CD11c was identified in all these cases. CD11c underexpression was independent of other aberrancies, including HLA-DR underexpression (n = 14), aberrant CD56 expression (n = 11), and underexpression of CD33, CD38, and CD14 (n = 6, 5, and 5, respectively), supporting the utility of CD11c expression status on monocytes in establishing a CMML diagnosis.
authors.affiliation: Dept of Pathology, Section of Hematopathology, University of Chicago Medical Center, TW055/MC0008, 5841 S Maryland Ave, Chicago, IL 60637; girish.venkataraman@uchospitals.edu.
authors.forename: Payal
authors.initials: P
authors.lastname: Sojitra
authors.forename: Pranav
authors.initials: P
authors.lastname: Gandhi
authors.forename: Priscilla
authors.initials: P
authors.lastname: Fitting
authors.forename: Ameet R
authors.initials: AR
authors.lastname: Kini
authors.forename: Serhan
authors.initials: S
authors.lastname: Alkan
authors.forename: Milind M
authors.initials: MM
authors.lastname: Velankar
authors.forename: Girish
authors.initials: G
authors.lastname: Venkataraman
mesh_terms: D015703:Antigens, CD / Q000097:blood; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology; D039521:CD11c Antigen / Q000097:blood*; D005260:Female; D006684:HLA-DR Antigens / Q000097:blood; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000150:complications / Q000378:metabolism / Q000473:pathology; D015477:Leukemia, Myelomonocytic, Chronic / Q000378:metabolism / Q000473:pathology*; D007964:Leukocytosis / Q000150:complications / Q000378:metabolism / Q000473:pathology; D008297:Male; D009000:Monocytes / Q000378:metabolism / Q000473:pathology*; D009367:Neoplasm Staging
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D039521:CD11c Antigen; D006684:HLA-DR Antigens
keywords: Chronic myelomonocytic leukemia; Flow cytometry; Reactive monocytosis