title: Multifunctional biodegradable polymer nanoparticles with uniform sizes: generation and in vitro anti-melanoma activity.
abstract: We present a simple, yet versatile strategy for the fabrication of uniform biodegradable polymer nanoparticles (NPs) with controllable sizes by a hand-driven membrane-extrusion emulsification approach. The size and size distribution of the NPs can be easily tuned by varying the experimental parameters, including initial polymer concentration, surfactant concentration, number of extrusion passes, membrane pore size, and polymer molecular weight. Moreover, hydrophobic drugs (e.g., paclitaxel (PTX)) and inorganic NPs (e.g., quantum dots (QDs) and magnetic NPs (MNPs)) can be effectively and simultaneously encapsulated into the polymer NPs to form the multifunctional hybrid NPs through this facile route. These PTX-loaded NPs exhibit high encapsulation efficiency and drug loading density as well as excellent drug sustained release performance. As a proof of concept, the A875 cell (melanoma cell line) experiment in vitro, including cellular uptake analysis by fluorescence microscope, cytotoxicity analysis of NPs, and magnetic resonance imaging (MRI) studies, indicates that the PTX-loaded hybrid NPs produced by this technique could be potentially applied as a multifunctional delivery system for drug delivery, bio-imaging, and tumor therapy, including malignant melanoma therapy.
authors.affiliation: Hubei Key Lab of Materials Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), Wuhan 430074,People's Republic of China.
authors.forename: Ruijing
authors.initials: R
authors.lastname: Liang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Xian
authors.initials: X
authors.lastname: Wu
authors.forename: Liyun
authors.initials: L
authors.lastname: Dong
authors.forename: Renhua
authors.initials: R
authors.lastname: Deng
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.forename: Martin
authors.initials: M
authors.lastname: Sullivan
authors.forename: Shanqin
authors.initials: S
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.forename: Juan
authors.initials: J
authors.lastname: Tao
authors.forename: Xiangliang
authors.initials: X
authors.lastname: Yang
authors.forename: Jintao
authors.initials: J
authors.lastname: Zhu
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D001673:Biodegradation, Environmental; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004705:Endocytosis / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D019344:Lactic Acid / Q000737:chemistry; D008027:Light; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008545:Melanoma / Q000188:drug therapy / Q000473:pathology*; D008856:Microscopy, Fluorescence; D017239:Paclitaxel / Q000494:pharmacology / Q000627:therapeutic use; D010316:Particle Size; D019047:Phantoms, Imaging; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry; D045663:Quantum Dots; D012542:Scattering, Radiation
chemical_list: D001672:Biocompatible Materials; D058185:Magnetite Nanoparticles; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D011142:Polyvinyl Alcohol; D017239:Paclitaxel