title: Release of growth factors from a reinforced collagen GAG matrix supplemented with platelet rich plasma: Influence on cultured human meniscal cells.
abstract: Damage to meniscal cartilage has been strongly linked to accelerated articular wear and consequently to osteoarthritis. Damage might be ameliorated by delivery of growth factors from platelet rich plasma (PRP) via a fiber reinforced collagen matrix designed for meniscal repair. PRP composition, release of growth factors, and influence on meniscal cell growth and gene expression were investigated. PRP was prepared using Harvest Smartprep (HS-PRP), Cascade Fibrinet (CF-PRP), and a simple centrifuge protocol (DC-PRP) from four donors each. CF-PRP had the highest ratio of platelets, with very few other blood cell types. HS-PRP had the highest total number of platelets but also contained high levels of red and white blood cells. Absorbed to collagen matrices HS-PRP released the highest levels of TGF-β1 and PDGF-AB with DC-PRP the most IGF-1. Cumulative release from collagen matrix was 48 ng/cm(3) IGF-1, 96 ng/cm(3) TGF-β1, and 9.6 ng/cm(3) PDGF-AB. Collagen matrix with PRP was able to increase meniscal cell number above peripheral whole blood and up-regulated gene expression of Aggrecan, Collagen type I (α1), and Elastin (3.3 ± 0.8-fold, 2.9 ± 0.6-fold, 4.0 ± 1.4-fold, respectively). Demonstrating that PRP combined with fiber reinforced collagen matrix could influence meniscal cells and might be of use for treating meniscal defects.
authors.affiliation: Orthopaedic Research Unit, University of Cambridge, Box 180, Addenbrooke's Hospital, Hills Road, Cambridge, CB2 0QQ, United Kingdom.
authors.forename: Daniel
authors.initials: D
authors.lastname: Howard
authors.forename: Jenny H
authors.initials: JH
authors.lastname: Shepherd
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Kew
authors.forename: Paula
authors.initials: P
authors.lastname: Hernandez
authors.forename: Siddhartha
authors.initials: S
authors.lastname: Ghose
authors.forename: John A
authors.initials: JA
authors.lastname: Wardale
authors.forename: Neil
authors.initials: N
authors.lastname: Rushton
mesh_terms: D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D024042:Collagen Type I / Q000096:biosynthesis; D000090602:Collagen Type I, alpha 1 Chain; D006025:Glycosaminoglycans; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis*; D008592:Menisci, Tibial / Q000166:cytology* / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis*; D053657:Platelet-Rich Plasma / Q000502:physiology*; D054457:Tissue Scaffolds; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis*
chemical_list: D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D006025:Glycosaminoglycans; D010982:Platelet-Derived Growth Factor; D053773:Transforming Growth Factor beta1; C095477:platelet-derived growth factor AB; D007334:Insulin-Like Growth Factor I; D003094:Collagen
keywords: PRP; meniscal cells; meniscal repair; platelet rich plasma; reinforced collagen matrix