title: Possible involvement of Hcn1 ion channel in learning and memory dysfunction in SAMP8 mice.
abstract: The senescence-accelerated mouse prone 8 (SAMP8) strain exhibits age-related learning and memory deficits (LMD) at 2 months of age. Combined linkage analysis of 264 F2 intercross SAMP8 × JF1 mice and RNA-seq analysis identified Hcn1 gene out of 29 genes in the LMD region on chromosome 13. Hcn1 in SAMP8 strain showed 15 times less polyglutamine repetition compared to Japanese fancy mouse 1 (JF1). Whole cell patch clamp analysis showed that Hcn1 ion conductivity was significantly lower in SAMP8 compared to that of JF1, which may be associated with learning and memory deficiency.
authors.affiliation: Laboratory of Molecular and Cellular Biology, Faculty of Science and Engineering, Graduate School, University of Toyama, 3190 Gofuku, Toyama-shi, Toyama 930-8555, Japan.
authors.forename: Maruf Mohammad
authors.initials: MM
authors.lastname: Akbor
authors.forename: Koji
authors.initials: K
authors.lastname: Tomobe
authors.forename: Tomomi
authors.initials: T
authors.lastname: Yamada
authors.forename: Juhyon
authors.initials: J
authors.lastname: Kim
authors.forename: Hiroki
authors.initials: H
authors.lastname: Mano
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kurosawa
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sasaki
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Nomura
authors.forename: Masaharu
authors.initials: M
authors.lastname: Isobe
mesh_terms: D000595:Amino Acid Sequence; D000704:Analysis of Variance; D000818:Animals; D033481:Chromosomes, Mammalian / Q000235:genetics; D003433:Crosses, Genetic; D005260:Female; D056726:Genetic Association Studies; D049452:Green Fluorescent Proteins / Q000378:metabolism; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels / Q000737:chemistry / Q000378:metabolism*; D007858:Learning; D008297:Male; D008569:Memory Disorders / Q000378:metabolism* / Q000503:physiopathology*; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D018408:Patch-Clamp Techniques; D010455:Peptides / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D015221:Potassium Channels / Q000737:chemistry / Q000378:metabolism*; D040641:Quantitative Trait Loci / Q000235:genetics
chemical_list: C578666:Hcn1 protein, mouse; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D010455:Peptides; D015221:Potassium Channels; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C097188:polyglutamine
keywords: Chromosome 13; Hcn1; Learning and memory deficiency; QTL analysis; RNA-seq analysis; SAMP8