title: Nonomuraea shaanxiensis sp. nov., a novel actinomycete isolated from a soil sample.
abstract: A novel actinomycete, strain NEAU-st1(T), was isolated from a soil sample collected in Shaanxi province, Northwest China and characterized using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that strain NEAU-st1(T) belongs to the genus Nonomuraea, being most closely related to Nonomuraea rosea GW12687(T) (98.91 %), Nonomuraea solani NEAU-Z6(T) (98.44 %), Nonomuraea rhizophila YIM67092(T)(98.24 %) and Nonomuraea monospora PT708(T) (98.02 %); similarities to sequences of other type strains of the genus Nonomuraea were lower than 98 %. Both tree-making algorithms used also supported the position that strain NEAU-st1(T) formed a distinct clade with its most closely related species. Morphological and physiological characteristics confirmed that the strain belongs to the genus Nonomuraea and distinguished it from its most closely related species. DNA-DNA hybridization further differentiated strain NEAU-st1(T) from its nearest phylogenetic neighbours. These results suggested that strain NEAU-st1(T) represents a novel species of the genus Nonomuraea, for which the name Nonomuraea shaanxiensis sp. nov. is proposed. The type strain is NEAU-st1(T) (=CGMCC 4.7096(T) = DSM 45877(T)).
authors.affiliation: School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.
authors.forename: Yuejing
authors.initials: Y
authors.lastname: Zhang
authors.forename: Junwei
authors.initials: J
authors.lastname: Zhao
authors.forename: Chongxi
authors.initials: C
authors.lastname: Liu
authors.forename: Yue
authors.initials: Y
authors.lastname: Shen
authors.forename: Feiyu
authors.initials: F
authors.lastname: Jia
authors.forename: Xiangjing
authors.initials: X
authors.lastname: Wang
authors.forename: Wensheng
authors.initials: W
authors.lastname: Xiang
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S