title: New insights on the Golgi complex of Tritrichomonas foetus.
abstract: Tritrichomonas foetus is a protist that causes bovine trichomoniasis and presents a well-developed Golgi. There are very few studies concerning the Golgi in trichomonads. In this work, monoclonal antibodies were raised against Golgi of T. foetus and used as a tool on morphologic and biochemical studies of this organelle. Among the antibodies produced, one was named mAb anti-Golgi 20.3, which recognized specifically the Golgi complex by fluorescence and electron microscopy. By immunoblotting this antibody recognized two proteins with 60 and 66 kDa that were identified as putative beta-tubulin and adenosine triphosphatase, respectively. The mAb 20.3 also recognized the Golgi complex of the Trichomonas vaginalis, a human parasite. In addition, the nucleotide coding sequences of these proteins were identified and included in the T. foetus database, and the 3D structure of the proteins was predicted. In conclusion, this study indicated: (1) adenosine triphosphatase is present in the Golgi, (2) ATPase is conserved between T. foetus and T. vaginalis, (3) there is new information concerning the nucleic acid sequences and protein structures of adenosine triphosphatase and beta-tubulin from T. foetus and (4) the mAb anti-Golgi 20.3 is a good Golgi marker and can be used in future studies.
authors.affiliation: Universidade Santa Úrsula, Rio de Janeiro, Brazil.
authors.forename: Ivone
authors.initials: I
authors.lastname: De Andrade Rosa
authors.affiliation: Instituto de Bioquímica Medica (Unidade de Espectrometria de Massa e Proteômica) - Universidade Federal do Rio de Janeiro e Instituto Nacional de Biologia Estrutural e Bioimagem - INBEB, Brazil.
authors.forename: Marjolly Brigido
authors.initials: MB
authors.lastname: Caruso
authors.affiliation: Instituto de Bioquímica Medica (Unidade de Espectrometria de Massa e Proteômica) - Universidade Federal do Rio de Janeiro e Instituto Nacional de Biologia Estrutural e Bioimagem - INBEB, Brazil.
authors.forename: Silas Pessini
authors.initials: SP
authors.lastname: Rodrigues
authors.affiliation: Instituto de Bioquímica Medica (Unidade de Espectrometria de Massa e Proteômica) - Universidade Federal do Rio de Janeiro e Instituto Nacional de Biologia Estrutural e Bioimagem - INBEB, Brazil.
authors.forename: Reinaldo Barros
authors.initials: RB
authors.lastname: Geraldo
authors.affiliation: Laboratório de Biologia Genômica e Molecular, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Luiza Wilges
authors.initials: LW
authors.lastname: Kist
authors.affiliation: Laboratório de Biologia Genômica e Molecular, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Mauricio Reis
authors.initials: MR
authors.lastname: Bogo
authors.affiliation: Laboratório Nacional de Computação Cientifica (LNCC/MCT), Petrópolis, Rio de Janeiro, Brazil.
authors.forename: Luiz
authors.initials: L
authors.lastname: Gonzaga
authors.affiliation: Laboratório Nacional de Computação Cientifica (LNCC/MCT), Petrópolis, Rio de Janeiro, Brazil.
authors.forename: Ana Tereza R
authors.initials: AT
authors.lastname: DE Vasconcelos
authors.affiliation: INCA - Grupo de Biologia Estrutural, Divisão de Biologia Celular, Instituto Nacional de Câncer, Rio de Janeiro, Brazil.
authors.forename: Jose Andres
authors.initials: JA
authors.lastname: Morgado-Díaz
authors.affiliation: Instituto de Bioquímica Medica (Unidade de Espectrometria de Massa e Proteômica) - Universidade Federal do Rio de Janeiro e Instituto Nacional de Biologia Estrutural e Bioimagem - INBEB, Brazil.
authors.forename: Russolina Benedeta
authors.initials: RB
authors.lastname: Zingali
authors.affiliation: Universidade Santa Úrsula, Rio de Janeiro, Brazil.
authors.forename: Marlene
authors.initials: M
authors.lastname: Benchimol
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000913:Antibodies, Protozoan / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology*; D001483:Base Sequence; D002417:Cattle; D005260:Female; D006056:Golgi Apparatus / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure*; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D008856:Microscopy, Fluorescence / Q000662:veterinary; D008958:Models, Molecular; D011529:Protozoan Infections, Animal / Q000469:parasitology*; D015800:Protozoan Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D014246:Trichomonas vaginalis / Q000201:enzymology / Q000276:immunology; D016835:Tritrichomonas foetus / Q000201:enzymology / Q000235:genetics / Q000276:immunology / Q000648:ultrastructure*
chemical_list: D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D000251:Adenosine Triphosphatases