title: Detergent-free mass spectrometry of membrane protein complexes.
abstract: We developed a method that allows release of intact membrane protein complexes from amphipols, bicelles and nanodiscs in the gas phase for observation by mass spectrometry (MS). Current methods involve release of membrane protein complexes from detergent micelles, which reveals subunit composition and lipid binding. We demonstrated that oligomeric complexes or proteins requiring defined lipid environments are stabilized to a greater extent in the absence of detergent.
authors.affiliation: Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, UK.
authors.forename: Jonathan T S
authors.initials: JT
authors.lastname: Hopper
authors.forename: Yvonne Ting-Chun
authors.initials: YT
authors.lastname: Yu
authors.forename: Dianfan
authors.initials: D
authors.lastname: Li
authors.forename: Alison
authors.initials: A
authors.lastname: Raymond
authors.forename: Mark
authors.initials: M
authors.lastname: Bostock
authors.forename: Idlir
authors.initials: I
authors.lastname: Liko
authors.forename: Victor
authors.initials: V
authors.lastname: Mikhailov
authors.forename: Arthur
authors.initials: A
authors.lastname: Laganowsky
authors.forename: Justin L P
authors.initials: JL
authors.lastname: Benesch
authors.forename: Martin
authors.initials: M
authors.lastname: Caffrey
authors.forename: Daniel
authors.initials: D
authors.lastname: Nietlispach
authors.forename: Carol V
authors.initials: CV
authors.lastname: Robinson
mesh_terms: D003902:Detergents / Q000737:chemistry*; D019852:Diacylglycerol Kinase / Q000737:chemistry; D004058:Diffusion; D004926:Escherichia coli / Q000737:chemistry / Q000201:enzymology; D029968:Escherichia coli Proteins / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006216:Halobacteriaceae / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry*; D008823:Micelles; D046529:Microscopy, Electron, Transmission / Q000379:methods; D009004:Monosaccharide Transport Proteins / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D025603:Sensory Rhodopsins / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D027981:Symporters / Q000737:chemistry
chemical_list: D003902:Detergents; D029968:Escherichia coli Proteins; C050221:LacY protein, E coli; D008055:Lipids; D008565:Membrane Proteins; D008823:Micelles; D009004:Monosaccharide Transport Proteins; D025603:Sensory Rhodopsins; D027981:Symporters; D049452:Green Fluorescent Proteins; D019852:Diacylglycerol Kinase