title: Quinone-mediated microbial synthesis of reduced graphene oxide with peroxidase-like activity.
abstract: The effects of different quinones on graphene oxide (GO) reduction by Shewanella oneidensis MR-1 and the peroxidase activity of the resultant reduced graphene oxide (QRGO) were studied. The presence of 100 μM anthraquinone-2-sulfonate (AQS), anthraquinone-2,6-disulfonate and 5-hydroxy-1,4-naphthoquinone could lead to 1.6-2.8-fold increase in GO reduction rate, whereas anthraquinone-2-carboxylate slowed down the reduction. The stimulating effects of AQS increased with the increase of its concentration (10-100 μM). The mediated effects were proved by direct GO reduction by microbially reduced AQS. The mediated reduction of GO to QRGO was characterized by UV-vis, XRD, FTIR, Raman spectra, XPS, TEM and AFM, respectively. The as-prepared QRGO possessed peroxidase-like activity, which could catalyze the oxidation of 3,3'5,5'-tetramethylbenzidine by H2O2, and followed Michealis-Menten kinetics. A colorimetric sensor for quantitative determination of glucose based on the peroxidase activity of QRGO was developed over a range of 1-120 μM with a detection limit of 1 μM.
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education, School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Guangfei
authors.initials: G
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Jiti
authors.initials: J
authors.lastname: Zhou
authors.forename: Aijie
authors.initials: A
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Ruofei
authors.initials: R
authors.lastname: Jin
authors.forename: Hong
authors.initials: H
authors.lastname: Lv
mesh_terms: D000880:Anthraquinones / Q000494:pharmacology; D003124:Colorimetry; D005947:Glucose / Q000032:analysis; D006108:Graphite / Q000378:metabolism*; D007700:Kinetics; D010084:Oxidation-Reduction / Q000187:drug effects; D010087:Oxides / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism*; D011809:Quinones / Q000494:pharmacology*; D020592:Shewanella / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000880:Anthraquinones; D010087:Oxides; D011809:Quinones; C023554:anthraquinone sulfonate; D006108:Graphite; D009195:Peroxidase; D005947:Glucose
keywords: Glucose detection; Graphene; Mediated reduction; Peroxidase; Shewanella oneidensis