title: Progesterone induces expression of Lrp2 in the murine uterus.
abstract: Progesterone (P4) and progesterone receptor (PR) have important functions in uterine environment. In previous studies, using high density DNA microarray analysis, we identified low density lipoprotein receptor-related protein 2 (Lrp2) is one of the genes upregulated by P4 and PR. In present studies, we examined the expression of Lrp2 through real-time PCR, in situ hybridization and immunohistochemistry by P4-PR response. Lrp2 mRNA transcript was significantly increased after P4 treatment in the luminal and glandular epithelium of the wild-type mice. However, Lrp2 expression was not observed in the progesterone receptor knock out (PRKO) mice treated with P4. The expression of Lrp2 expression is not regulated by estrogen. During early pregnancy, the expression of Lrp2 was detected at 2.5 dpc and then significantly increased at 3.5 dpc in luminal and glandular epithelium. These results suggest that Lrp2 is a novel target gene by P4 and PR.
authors.affiliation: Department of Obstetrics and Gynecology & Reproductive Biology, Michigan State University, College of Human Medicine, Grand Rapids, MI 49503, United States; Major in Biomodulation, Seoul National University, Seoul, Republic of Korea.
authors.forename: Seo Jin
authors.initials: SJ
authors.lastname: Oh
authors.forename: Tae Hoon
authors.initials: TH
authors.lastname: Kim
authors.forename: Jeong Mook
authors.initials: JM
authors.lastname: Lim
authors.forename: Jae-Wook
authors.initials: JW
authors.lastname: Jeong
mesh_terms: D000818:Animals; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D026561:Low Density Lipoprotein Receptor-Related Protein-2 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology*; D011555:Pseudopregnancy / Q000378:metabolism / Q000473:pathology; D014599:Uterus / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology
chemical_list: D026561:Low Density Lipoprotein Receptor-Related Protein-2; C055127:Lrp2 protein, mouse; D011374:Progesterone; D004958:Estradiol
keywords: Lrp2; Progesterone; Progesterone receptor; Uterus