title: Bone biomarkers in patients with chronic traumatic spinal cord injury.
abstract: BACKGROUND
  Bone loss after spinal cord injury (SCI) occurs because of pathologic changes in osteoblastic and osteoclastic activities due to mechanical unloading. Some biochemical changes in bone metabolism after SCI are described before that were related to bone mineral loss.
  
  
  OBJECTIVE
  Our purpose was to determine bone markers' changes and related effective factors in patients with chronic traumatic SCI.
  
  
  METHODS
  This investigation was designed as an observational cross-sectional study.
  
  
  METHODS
  All patients with chronic SCI who were referred to Brain and Spinal Injury Research Center and did not meet our exclusion criteria entered the study.
  
  
  METHODS
  Self-reporting measures including patient's demographic features and date of accident were obtained using a questionnaire and physiologic measures including spinal magnetic resonance imaging to determine the level of injury accompanied with physical examination along with dual-energy X-ray absorptiometry were performed. Blood samples were analyzed in the laboratory.
  
  
  METHODS
  Dual-energy X-ray was used to determine bone mineral density in femoral and spinal vertebrae bone sites. Serum level of C-telopeptide cross-linked Type 1 collagen (CTX), parathyroid hormone, calcitonin, osteocalcin, and bone alkaline phosphatase (BALP) were measured.
  
  
  RESULTS
  We detected a negative association between CTX level and bone mineral density in femoral and spinal bone sites that confirms that CTX is a bone resorption marker. C-telopeptide cross-linked Type 1 collagen and BALP levels did not show any significant correlation with postduration injury. Patients with spinal injury at lumbar level had the highest calcitonin level (p<.04). C-telopeptide cross-linked Type 1 collagen was positively related with osteocalcin and BALP (p<.0001, r=0.51), and osteocalcin was positively related with BALP (p<.0001, r=0.44). Osteocalcin was related negatively only to femoral intertrochanteric zone bone mineral density.
  
  
  CONCLUSIONS
  Some bone biomarkers undergo noticeable changes after SCI. C-telopeptide cross-linked Type 1 collagen was positively correlated with BALP and osteocalcin that shows the coincidental occurrence of osteoblastic and osteoclastic activities. Our data also support this fact that although bone reduction after 2 years is slower than acute phase after SCI, bone resorption rate is higher than bone formation. These bone markers also revealed different site of action as osteocalcin level only affected femoral intertrochanteric bone mineral density. Generally, it seems that the coincidental consideration of these factors that influence bone mineral density can lead to a better understanding of bone changes after SCI.
authors.affiliation: Brain and Spinal Injury (BASIR) Research Center, Imam Khomeini Hospital, Tehran University of Medical Sciences, Keshavarz Avenu, Tehran, Iran.
authors.forename: Hadis
authors.initials: H
authors.lastname: Sabour
authors.affiliation: Brain and Spinal Injury (BASIR) Research Center, Imam Khomeini Hospital, Tehran University of Medical Sciences, Keshavarz Avenu, Tehran, Iran. Electronic address: noroozia@tums.ac.ir.
authors.forename: Abbas
authors.initials: A
authors.lastname: Norouzi Javidan
authors.affiliation: Brain and Spinal Injury (BASIR) Research Center, Imam Khomeini Hospital, Tehran University of Medical Sciences, Keshavarz Avenu, Tehran, Iran.
authors.forename: Sahar
authors.initials: S
authors.lastname: Latifi
authors.affiliation: Endocrinology and Metabolism Research Institute (EMRI), Endocrinology Department, Tehran University of Medical Sciences, North Kargar St, Tehran, Iran.
authors.forename: Bagher
authors.initials: B
authors.lastname: Larijani
authors.affiliation: Department of Nutrition, Iran University of Medical Sciences, Hemat Highway, Tehran, Iran.
authors.forename: Farzad
authors.initials: F
authors.lastname: Shidfar
authors.affiliation: Department of Nutrition, Iran University of Medical Sciences, Hemat Highway, Tehran, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Vafa
authors.affiliation: Endocrinology and Metabolism Research Institute (EMRI), Endocrinology Department, Tehran University of Medical Sciences, North Kargar St, Tehran, Iran.
authors.forename: Ramin
authors.initials: R
authors.lastname: Heshmat
authors.affiliation: Brain and Spinal Injury (BASIR) Research Center, Imam Khomeini Hospital, Tehran University of Medical Sciences, Keshavarz Avenu, Tehran, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Emami Razavi
mesh_terms: D000328:Adult; D000368:Aged; D000469:Alkaline Phosphatase / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D015519:Bone Density; D002116:Calcitonin / Q000097:blood*; D024042:Collagen Type I / Q000097:blood*; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015675:Osteocalcin / Q000097:blood*; D010281:Parathyroid Hormone / Q000097:blood*; D010455:Peptides / Q000097:blood*; D013119:Spinal Cord Injuries / Q000097:blood*
chemical_list: D015415:Biomarkers; D024042:Collagen Type I; D010281:Parathyroid Hormone; D010455:Peptides; C052929:collagen type I trimeric cross-linked peptide; D015675:Osteocalcin; D002116:Calcitonin; D000469:Alkaline Phosphatase
keywords: Bone alkaline phosphatase; Bone biomarkers; Bone mineral density; Spinal cord injury