title: An NA-deficient 2009 pandemic H1N1 influenza virus mutant can efficiently replicate in cultured cells.
abstract: We identified a novel neuraminidase (NA)-deficient virus that was a 2009 pandemic influenza H1N1 virus mutant. The mutant virus had a deletion of 1,009 nt in the NA gene and lacked an enzymatic domain. Although the yield of the NA-deficient virus was limited, it formed large plaques when applied to MDCK cell cultures, indicating that the virus was able to spread to adjacent cells. Furthermore, the NA-deficient virus was eluted from chicken erythrocytes at 37 °C, even in the presence of the antiviral drug peramivir. Spread of this NA-deficient virus may pose a potential threat to anti-influenza therapies.
authors.affiliation: Department of Microbiology and Immunology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.
authors.forename: Emi
authors.initials: E
authors.lastname: Inoue
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ieko
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Takahashi
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Osawa
authors.forename: Katsunori
authors.initials: K
authors.lastname: Okazaki
mesh_terms: D000818:Animals; D002460:Cell Line; D002645:Chickens; D004285:Dogs; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D007251:Influenza, Human / Q000821:virology; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000172:deficiency*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D010948:Viral Plaque Assay; D014764:Viral Proteins; D014779:Virus Replication
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins; C487630:NA protein, influenza A virus; D009439:Neuraminidase