title: Developmental regulation of neuroligin genes in Japanese ricefish (Oryzias latipes) embryogenesis maintains the rhythm during ethanol-induced fetal alcohol spectrum disorder.
abstract: Although prenatal alcohol exposure is the potential cause of fetal alcohol spectrum disorder (FASD) in humans, the molecular mechanism(s) of FASD is yet unknown. We have used Japanese ricefish (Oryzias latipes) embryogenesis as an animal model of FASD and reported that this model has effectively generated several phenotypic features in the cardiovasculature and neurocranial cartilages by developmental ethanol exposure which is analogous to human FASD phenotypes. As FASD is a neurobehavioral disorder, we are searching for a molecular target of ethanol that alters neurological functions. In this communication, we have focused on neuroligin genes (nlgn) which are known to be active at the postsynaptic side of both excitatory and inhibitory synapses of the central nervous system. There are six human NLGN homologs of Japanese ricefish reported in public data bases. We have partially cloned these genes and analyzed their expression pattern during normal development and also after exposing the embryos to ethanol. Our data indicate that the expression of all six nlgn genes in Japanese ricefish embryos is developmentally regulated. Although ethanol is able to induce developmental abnormalities in Japanese ricefish embryogenesis comparable to the FASD phenotypes, quantitative real-time PCR (qPCR) analysis of nlgn mRNAs indicate unresponsiveness of these genes to ethanol. We conclude that the disruption of the developmental rhythm of Japanese ricefish embryogenesis by ethanol that leads to FASD may not affect the nlgn gene expression at the message level.
authors.affiliation: Department of Pharmacology, University of Mississippi, University, MS 38677, USA.
authors.forename: Mona H
authors.initials: MH
authors.lastname: Haron
authors.forename: Ikhlas A
authors.initials: IA
authors.lastname: Khan
authors.forename: Asok K
authors.initials: AK
authors.lastname: Dasmahapatra
mesh_terms: D000818:Animals; D047108:Embryonic Development / Q000235:genetics*; D000431:Ethanol / Q000009:adverse effects*; D063647:Fetal Alcohol Spectrum Disorders / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D023421:Models, Animal; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009990:Oryzias / Q000196:embryology* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D000431:Ethanol
keywords: Development; Ethanol; Fetal alcohol spectrum disorder; Japanese ricefish; Neuroligin