title: Genetic dissection of complete genomes of Type 2 PRRS viruses isolated in Denmark over a period of 15 years.
abstract: Type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) was first detected in Europe in 1996 co-incident with the introduction of a live attenuated vaccine. Since then, only limited ORF5 and ORF7 sequences of Type 2 PRRS viruses have been reported throughout Europe. In the present study, the genetic and antigenic diversity of 11 complete genomes and 49 ORF5 and 55 ORF7 nucleotide sequences obtained from 57 viruses in Denmark from 2003 to 2012 were examined. The genetic identity of the 11 complete genomes to the vaccine strain (Ingelvac PRRS MLV) ranged between 93.6 and 99.6% while the 49 ORF5 sequences examined were 94.0-99.8% identical to the vaccine strain. Among the Danish sequences, the pairwise nucleotide identity was 90.9-100% and 93.0-100.0% for ORF5 and ORF7, respectively. Analysis of the genetic region encoding NSP2 revealed high diversity among the Danish viruses with an 86.6-98.9% range in similarity. Furthermore, several of the sequenced viruses harbored deletions in the NSP2 coding region. Phylogenetic analysis in a global Type 2 PRRSV framework classified all Danish isolates to a single cluster (sub-lineage 5.1) which comprised strains closely-related to the Type 2 prototype isolate VR2332.
authors.affiliation: National Veterinary Institute, Technical University of Denmark, DK-1870 Frederiksberg C, Denmark. Electronic address: likik@vet.dtu.dk.
authors.forename: Lise K
authors.initials: LK
authors.lastname: Kvisgaard
authors.forename: Charlotte K
authors.initials: CK
authors.lastname: Hjulsager
authors.forename: Manreetpal Singh
authors.initials: MS
authors.lastname: Brar
authors.forename: Frederick C C
authors.initials: FC
authors.lastname: Leung
authors.forename: Lars E
authors.initials: LE
authors.lastname: Larsen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D003718:Denmark; D005060:Europe; D014644:Genetic Variation; D016679:Genome, Viral / Q000235:genetics*; D006023:Glycoproteins / Q000737:chemistry; D008969:Molecular Sequence Data; D010802:Phylogeny; D019318:Porcine Reproductive and Respiratory Syndrome / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016415:Sequence Alignment; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics; D014765:Viral Vaccines / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D006023:Glycoproteins; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines
keywords: Amino acid variations ORF5; Full genome/complete genome; Genotype 2/Type 2; Non-structural protein 2 (nsp2); Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)