title: Monitoring of children with acute gastroenteritis in Madrid, Spain, during 2010-2011: rotavirus genotype distribution after the vaccines introduction.
abstract: OBJECTIVE
  A structured surveillance study was conducted on children with diarrhea who were hospitalized in Madrid (Spain) during 2010-2011, in order to describe temporal, geographic, and age-related trends in rotavirus (RV) strains after the introduction of the RV vaccines in our country.
  
  
  RESULTS
  A total of 370 children were enrolled, with RV being detected in 117 (31.6%) cases. Coinfections were detected mainly with rotavirus, astrovirus and norovirus. The most prevalent rotavirus G type was G1 (60.7%) followed by G2 (16.09%), G9 (5.9%), and G12 (5.1%). The G12 genotype appeared for the first time in 2008 in Spain, and it has increased to 5.1% of the cases in this report. Some uncommon P genotypes, such as P[14] and P[6], both with a low percentage, were found. The samples with G1 G2, G9 and G12 genotypes appeared in all ages, but were significantly higher in children under 2 years old.
  
  
  CONCLUSIONS
  A long-term structured surveillance is required in the Spanish post vaccine era, in order to determine the prevalence and variability of RV genotypes. This will especially be needed to distinguish between changes occurring as a result of natural fluctuation in genotype or those (changes) that could be mediated by population immunity to the vaccines. In addition, it will be necessary to study the impact of the current vaccines on the circulating rotavirus strains and on the overall reduction in the prevalence of rotavirus disease among children in Spain.
authors.affiliation: Viral Gastroenteritis Unit, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain. Electronic address: asanchez@isciii.es.
authors.forename: Alicia
authors.initials: A
authors.lastname: Sánchez-Fauquier
authors.affiliation: Hospital Virgen del Rocío, Sevilla, Spain.
authors.forename: Verónica
authors.initials: V
authors.lastname: González-Galán
authors.affiliation: Viral Gastroenteritis Unit, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Sandra
authors.initials: S
authors.lastname: Arroyo
authors.affiliation: Viral Gastroenteritis Unit, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Cabornero
authors.affiliation: Hospital Severo Ochoa, Leganés, Madrid, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Ruiz-Burruecos
authors.affiliation: Hospital Severo Ochoa, Leganés, Madrid, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Wilhelmi-De Cal
mesh_terms: D000208:Acute Disease; D002675:Child, Preschool; D062665:Epidemiological Monitoring; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology* / Q000517:prevention & control / Q000821:virology*; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D015995:Prevalence; D012401:Rotavirus / Q000235:genetics*; D022243:Rotavirus Vaccines; D013030:Spain / Q000453:epidemiology; D013997:Time Factors; D014504:Urban Health
chemical_list: D022243:Rotavirus Vaccines
keywords: Diarrea; Diarrhea; Genotipos rotavirus; Rotavirus genotypes; Rotavirus vaccines; Vacuna rotavirus