title: Antisense oligodeoxynucleotide-mediated gene knockdown in pollen tubes.
abstract: Specific gene knockdown mediated by the antisense oligodeoxynucleotides (AODNs) strategy recently emerged as a rapid and effective tool for probing gene role in plant cells, particularly tip-growing pollen tubes. Here, we describe the protocol for the successful employment of AODN technique in growing tobacco pollen tubes, covering AODN design, application, and analysis of the results. We also discuss the advantages and drawbacks of this method.
authors.affiliation: Department of Experimental Plant Biology, Faculty of Science, Charles University and Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Radek
authors.initials: R
authors.lastname: Bezvoda
authors.forename: Roman
authors.initials: R
authors.lastname: Pleskot
authors.forename: Viktor
authors.initials: V
authors.lastname: Zárský
authors.forename: Martin
authors.initials: M
authors.lastname: Potocký
mesh_terms: D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D010641:Phenotype; D053205:Pollen Tube / Q000235:genetics* / Q000254:growth & development; D014026:Nicotiana / Q000235:genetics / Q000254:growth & development
chemical_list: D016376:Oligonucleotides, Antisense