title: Time-lapse AFM imaging of DNA conformational changes induced by daunorubicin.
abstract: Cancer is a major health issue that absorbs the attention of a large part of the biomedical research. Intercalating agents bind to DNA molecules and can inhibit their synthesis and transcription; thus, they are increasingly used as drugs to fight cancer. In this work, we show how atomic force microscopy in liquid can characterize, through time-lapse imaging, the dynamical influence of intercalating agents on the supercoiling of DNA, improving our understanding of the drug's effect.
authors.affiliation: Laboratoire de Physique de la Matière Vivante, École Polytechnique Fédérale de Lausanne (EPFL) , CH-1015 Lausanne, Switzerland.
authors.forename: Livan
authors.initials: L
authors.lastname: Alonso-Sarduy
authors.forename: Giovanni
authors.initials: G
authors.lastname: Longo
authors.forename: Giovanni
authors.initials: G
authors.lastname: Dietler
authors.forename: Sandor
authors.initials: S
authors.lastname: Kasas
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000187:drug effects / Q000648:ultrastructure; D003630:Daunorubicin / Q000494:pharmacology*; D015195:Drug Design; D006801:Humans; D007364:Intercalating Agents / Q000494:pharmacology; D018625:Microscopy, Atomic Force; D009690:Nucleic Acid Conformation / Q000187:drug effects*
chemical_list: D007364:Intercalating Agents; D004247:DNA; D003630:Daunorubicin