title: A highly selective fluorescence "turn-on" probe for Cu(II) based on reaction and its imaging in living cells.
abstract: A new oxidative C-O bond cleavage reaction-based probe FluHMPP was designed and prepared. FluHMPP displays excellent selective turn-on fluorescence response for Cu(II) in aqueous solution under visible light excitation. The cleavage products are fully characterized. Fluorescein fragment is further oxidized to highly fluorescent MFME (3'-O-methylfluorescein methyl ester), and benzyl ether of imine fragment has been transformed to carboxyl. Confocal microscopy experiments have demonstrated that FluHMPP could also be used in live cells for the detection of Cu(II).
authors.affiliation: Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou 730000, People's Republic of China.
authors.forename: Zhaohua
authors.initials: Z
authors.lastname: Shi
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Tang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhou
authors.forename: Ju
authors.initials: J
authors.lastname: Cheng
authors.forename: Qingxin
authors.initials: Q
authors.lastname: Han
authors.forename: Ji-an
authors.initials: JA
authors.lastname: Zhou
authors.forename: Bei
authors.initials: B
authors.lastname: Wang
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Yang
authors.forename: Weisheng
authors.initials: W
authors.lastname: Liu
authors.forename: Decheng
authors.initials: D
authors.lastname: Bai
mesh_terms: D019817:Benzoic Acid / Q000737:chemistry*; D003300:Copper / Q000032:analysis* / Q000737:chemistry; D018360:Crystallography, X-Ray; D019793:Fluorescein / Q000032:analysis / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018613:Microscopy, Confocal; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence; D014966:Xanthenes / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D014966:Xanthenes; D003300:Copper; D019817:Benzoic Acid; D019793:Fluorescein