title: 2,3-Butanediol recovery from fermentation broth by alcohol precipitation and vacuum distillation.
abstract: This study presents a new and effective downstream process to recover 2,3-butanediol (2,3-BD) from fermentation broth which is produced by a recombinant Klebsiella pneumoniae strain. The ldhA-deficient K. pneumoniae strain yielded about 90 g/L of 2,3-BD, along with a number of by-products, such as organic acids and alcohols, in a 65 h fed-batch fermentation. The pH-adjusted cell-free fermentation broth was firstly concentrated until 2,3-BD reached around 500 g/L by vacuum evaporation at 50°C and 50 mbar vacuum pressure. The concentrated solution was further treated using light alcohols, including methanol, ethanol, and isopropanol, for the precipitation of organic acids and inorganic salts. Isopropanol showed the highest removal efficiency, in which 92.5% and 99.8% of organic acids and inorganic salts were precipitated, respectively. At a final step, a vacuum distillation process enabled the recovery of 76.2% of the treated 2,3-BD, with 96.1% purity, indicating that fermentatively produced 2,3-BD is effectively recovered by a simple alcohol precipitation and vacuum distillation.
authors.affiliation: Research and Development Center, GS Caltex Corporation, 104-4 Munji-dong, Yuseong-gu, Daejeon 305-380, Republic of Korea.
authors.forename: Sangjun
authors.initials: S
authors.lastname: Jeon
authors.affiliation: Research and Development Center, GS Caltex Corporation, 104-4 Munji-dong, Yuseong-gu, Daejeon 305-380, Republic of Korea; BioSystem Engineering Laboratory, Department of Chemical and Biomolecular Engineering, KAIST, Yuseong-gu, Daejeon 305-704, Republic of Korea.
authors.forename: Duk-Ki
authors.initials: DK
authors.lastname: Kim
authors.affiliation: Research and Development Center, GS Caltex Corporation, 104-4 Munji-dong, Yuseong-gu, Daejeon 305-380, Republic of Korea. Electronic address: hyohaks@gscaltex.com.
authors.forename: Hyohak
authors.initials: H
authors.lastname: Song
authors.affiliation: Research and Development Center, GS Caltex Corporation, 104-4 Munji-dong, Yuseong-gu, Daejeon 305-380, Republic of Korea.
authors.forename: Hee Jong
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Chemical and Biomolecular Engineering, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Sunghoon
authors.initials: S
authors.lastname: Park
authors.affiliation: Research and Development Center, GS Caltex Corporation, 104-4 Munji-dong, Yuseong-gu, Daejeon 305-380, Republic of Korea.
authors.forename: Doyoung
authors.initials: D
authors.lastname: Seung
authors.affiliation: BioSystem Engineering Laboratory, Department of Chemical and Biomolecular Engineering, KAIST, Yuseong-gu, Daejeon 305-704, Republic of Korea.
authors.forename: Yong Keun
authors.initials: YK
authors.lastname: Chang
mesh_terms: D000143:Acids; D000438:Alcohols / Q000737:chemistry*; D061249:Batch Cell Culture Techniques; D019149:Bioreactors; D002072:Butylene Glycols / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D011232:Chemical Precipitation; D057168:Distillation; D005285:Fermentation; D017353:Gene Deletion; D006863:Hydrogen-Ion Concentration; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000172:deficiency / Q000235:genetics; D012492:Salts; D014618:Vacuum
chemical_list: D000143:Acids; D000438:Alcohols; D002072:Butylene Glycols; D012492:Salts; C026978:2,3-butylene glycol; D007770:L-Lactate Dehydrogenase
keywords: 2,3-Butanediol; Alcohol precipitation; Fermentation; Klebsiella pneumoniae; Recovery