title: Different antigenicities of the N-terminal region of cellular and scrapie prion proteins.
abstract: Limited information is available about conformational differences between the abnormal isoform of prion protein (PrP(Sc) ) and cellular prion protein (PrP(C) ) under native conditions. To clarify conformational differences between these two isoforms, PrP-deficient mice were immunized with brain homogenates of normal and scrapie-infected animals. All mice generated anti-PrP antibodies. Peptide array analysis of these serum samples revealed a distinctive epitope of PrP(Sc) consisting of QGSPGGN (PrP41-47) at the N-terminus. This study demonstrated a conformational dissimilarity at the N-terminus between PrP(Sc) and PrP(C) , a finding that may provide novel information about conformational features of PrP(Sc) .
authors.affiliation: Nippi Research Institute of Biomatrix, Kuwabara 520-11, Toride, Ibaraki, 302-0017.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ushiki-Kaku
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Iwamaru
authors.forename: Kentaro
authors.initials: K
authors.lastname: Masujin
authors.forename: Morikazu
authors.initials: M
authors.lastname: Imamura
authors.forename: Shigeyoshi
authors.initials: S
authors.lastname: Itohara
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Ogawa-Goto
authors.forename: Shunji
authors.initials: S
authors.lastname: Hattori
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokoyama
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D001921:Brain / Q000276:immunology; D006224:Cricetinae; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D007114:Immunization; D051379:Mice; D018621:PrPC Proteins / Q000737:chemistry* / Q000276:immunology*; D018620:PrPSc Proteins / Q000737:chemistry* / Q000276:immunology*; D012608:Scrapie / Q000276:immunology; D012756:Sheep
chemical_list: D000939:Epitopes; D018621:PrPC Proteins; D018620:PrPSc Proteins
keywords: N-terminal region; abnormal isoform of prion protein; antigenicity; prion