title: Aberrant expression of laminin γ2 correlates with poor prognosis and promotes invasion in extrahepatic cholangiocarcinoma.
abstract: BACKGROUND
  To investigate the potential role of laminin γ2 and its correlation with prognosis in patients with extrahepatic cholangiocarcinoma (CCA).
  
  
  METHODS
  Laminin γ2 expression was evaluated by immunohistochemistry in 72 extrahepatic CCA patients after surgical resection. Knockdown of laminin γ2 was achieved via small interfering RNA transfection in the extrahepatic CCA cell line QBC939.
  
  
  RESULTS
  Thirty-six of 72 extrahepatic CCAs (50%) stained positive for laminin γ2 in two types of patterns: stromal staining (28/72, 39%) and cytoplasmic staining (24/72, 33%). All 16 paracancerous tissue samples showed negative staining. Both stromal and cytoplasmic laminin γ2 expressions correlated with lymph node metastasis. Kaplan-Meier analysis showed that aberrant expression of laminin γ2 correlated with poor overall survival and early recurrence. Cox regression analysis further demonstrated that laminin γ2 expression was a significant independent predictor of poor overall survival and early recurrence. Immunofluorescence staining revealed cytoplasmic expression of laminin γ2 in QBC939 cells. Knockdown of laminin γ2 significantly reduced QBC939 cell invasion and migration.
  
  
  CONCLUSIONS
  Aberrant expression of laminin γ2 correlates with poor prognosis and promotes invasion in extrahepatic CCA.
authors.affiliation: Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing, China; Department of Infectious Disease, 324 Hospital of People's Liberation Army (PLA), Chongqing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Tian
authors.forename: Peng
authors.initials: P
authors.lastname: Jiang
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.forename: Xiaowu
authors.initials: X
authors.lastname: Li
authors.forename: Shuguang
authors.initials: S
authors.lastname: Wang
mesh_terms: D000368:Aged; D001650:Bile Duct Neoplasms / Q000737:chemistry / Q000401:mortality* / Q000473:pathology; D017734:Bile Ducts, Extrahepatic; D045744:Cell Line, Tumor; D002465:Cell Movement; D018281:Cholangiocarcinoma / Q000737:chemistry / Q000401:mortality* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009364:Neoplasm Recurrence, Local; D011379:Prognosis
chemical_list: C400567:LAMC2 protein, human; D007797:Laminin
keywords: Cholangiocarcinoma; Invasion; Laminin γ2; Prognosis