title: A biorepository for ophthalmic surgical specimens.
abstract: Biorepositories are collections of surgically obtained human tissues for current and future investigations of disease mechanisms, therapeutics, and diagnostics. In ophthalmology, a critical challenge is how to interface the operating room with the laboratory. To attain standards required for basic research, clinical and research teams must cooperate to collect, annotate, and store specimens that yield consistent results required for advanced molecular techniques. We developed an efficient platform for obtaining vitreous and other eye tissues from the operating room and transferring them to the lab. The platform includes a mobile lab cart for on-site tissue processing, a multi-user, web-based database for point-of-care phenotypic capture, and an integrated data tracking system for long-term storage. These biorepository instruments have proven essential for our studies in ophthalmic disease proteomics. This system can be implemented in other operating rooms and laboratories for a variety of biological tissues.
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, IA, USA; Omics Laboratory, University of Iowa, Iowa City, IA, USA.
authors.forename: Jessica M
authors.initials: JM
authors.lastname: Skeie
authors.forename: Stephen H
authors.initials: SH
authors.lastname: Tsang
authors.forename: Ryan Vande
authors.initials: RV
authors.lastname: Zande
authors.forename: Macy M
authors.initials: MM
authors.lastname: Fickbohm
authors.forename: Shaival S
authors.initials: SS
authors.lastname: Shah
authors.forename: John G
authors.initials: JG
authors.lastname: Vallone
authors.forename: Vinit B
authors.initials: VB
authors.lastname: Mahajan
mesh_terms: D016208:Databases, Factual; D005125:Eye Banks; D006801:Humans; D016247:Information Storage and Retrieval; D012106:Research; D013048:Specimen Handling; D014015:Tissue Banks; D014822:Vitreous Body
keywords: Biobank; Biorepository; Retina; Vitreous