title: Reticulated bioactive scaffolds with improved textural properties for bone tissue engineering: nanostructured surfaces and porosity.
abstract: Organised nanoporous SBA-15 type silica precursor (SP) particulate material has been processed into three-dimensional macroporous, reticulated structures using a novel strategy consisting of blending increasing percentages of SP with a SiO2 -CaO-P2 O5 (80Si15Ca5P) mesoporous bioactive glass (MBG) sol. The procedure successfully produced consolidated and functionally competent open-cell scaffolds while preserving the nanoporous order of the SP. Scaffolds were prepared using four different (MBG)/(SP) ratios. These structures were then characterized using field emission gun scanning electron microscopy, X-ray diffraction (XRD), nitrogen adsorption-desorption measurements, and compressive strength testing. Open-cell interconnected structures with dual macro (150-500 μm) and nano (4-6 nm)-organised porosity were produced. Both the textural and mechanical properties were found to improve with increasing SBA-15 content. The in vitro bioactive response using simulated body fluid confirmed high reactivity for all prepared scaffolds. In addition, the SBA-15 containing scaffolds exhibited a superior ability to delay the pH-triggered lysozyme release with antibiotic activity.
authors.affiliation: Instituto de Ciencia de Materiales de Sevilla (ICMS), Centro Mixto CSIC-Universidad de Sevilla, Avda. Américo Vespucio 49, Isla de la Cartuja, 41092, Seville, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain.
authors.forename: M Lourdes
authors.initials: ML
authors.lastname: Ramiro-Gutiérrez
authors.forename: Julia
authors.initials: J
authors.lastname: Will
authors.forename: Aldo R
authors.initials: AR
authors.lastname: Boccaccini
authors.forename: Aránzazu
authors.initials: A
authors.lastname: Díaz-Cuenca
mesh_terms: D018786:Bone Substitutes / Q000737:chemistry*; D002516:Ceramics / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009113:Muramidase / Q000008:administration & dosage; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: C022107:Bioglass; D018786:Bone Substitutes; D003692:Delayed-Action Preparations; C509969:SBA-15; D012822:Silicon Dioxide; D009113:Muramidase
keywords: SBA-15 scaffolding processing; bioactivity; dual macro nano-organised porous structure; enzyme carrier; reticulated scaffold