title: Inhibition of hepatocellular carcinoma growth by adenovirus-mediated expression of human telomerase reverse transcriptase COOH-27 terminal polypeptide in mice.
abstract: A 27-kDa C-terminal fragment of human telomerase reverse transcriptase, hTERTC27, has previously been reported to inhibit the growth and tumorigenicity of HeLa human cervical cancer cells and U87-MG human glioblastoma multiforme cells. However, the antitumor effects of hTERTC27 in hepatoma and its underlying mechanisms are unclear. In the current study, the therapeutic effect of hTERTC27, mediated by recombinant adenovirus, in hepatocellular carcinoma (HCC) was exploredin vitroandin vivoto investigate the possible mechanisms. The results indicated that recombinant adenovirus carrying hTERTC27 (rAdv-hTERTC27) effectively inhibited the growth and induced apoptosis of the Hepa 1-6 HCC cells. Dendritic cells transduced with rAdv-hTERTC27 were highly effective at inducing antigen-specific T cell proliferation and increasing the activated cytotoxicity of T cells against Hepa 1-6 cells. HCC was inhibited significantly when a single dose of 5×107pfu rAdv-hTERTC27 was administered intravenously. In summary, the results of this study demonstrated that rAdv-hTERTC27 may serve as a reagent for intravenous administration when combined with telomerase-based gene therapy and immunotherapy for cancer.
authors.affiliation: Department of Neurology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510120, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: He
authors.forename: Han-Xian
authors.initials: HX
authors.lastname: Gong
authors.forename: Xiang-Pen
authors.initials: XP
authors.lastname: Li
authors.forename: Yi-Dong
authors.initials: YD
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Jun-Jian
authors.initials: JJ
authors.lastname: Huang
authors.forename: Dan
authors.initials: D
authors.lastname: Xie
authors.forename: Hsiang-Fu
authors.initials: HF
authors.lastname: Kung
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
keywords: cytotoxic T lymphocytes; gene therapy; hTERTC27; hepatocellular carcinoma; immunotherapy