title: Molecular detection of Ehrlichia canis and Anaplasma platys in dogs in Southern Brazil.
abstract: The aims of this study were to determine the occurrence of Anaplasma platys and Ehrlichia canis infection in dogs in Porto Alegre, Southern Brazil; and to investigate their association with hematological abnormalities. Serum samples from 196 dogs were first tested using dot-ELISA for antibodies against Anaplasma spp. and Ehrlichia canis. Peripheral blood samples from 199 dogs were subjected to 16S rRNA nested PCR (nPCR) for A. platys and E. canis, followed by DNA sequencing to ensure pathogen identity. A total of 19/196 samples (9.69%) were positive for Anaplasma spp. using ELISA and 28/199 (14.07%) samples were positive for A. platys by nested PCR. All the dog samples were negative for E. canis, both in anti-E. canis antibody tests and in nested PCR. There were no significant differences in hematological parameters between A. platys-PCR positive and negative dogs and Anaplasma spp. serologically positive dogs, except for basophil counts, which were higher in nPCR-positive dogs. This is the first report showing A. platys presence in dogs in Southern Brazil. In conclusion, hematological parameters may not be sufficient to diagnose A. platys infection in dogs in Southern Brazil, probably due either to low pathogenicity or to chronic infection. On the other hand, E. canis may either have very low occurrence or be absent in dogs in Porto Alegre.
authors.affiliation: Universidade Federal do Rio Grande do Sul, Departamento de Patologia Clínica Veterinária, Porto AlegreRS, Brasil.
authors.forename: Camila Serina
authors.initials: CS
authors.lastname: Lasta
authors.forename: Andrea Pires
authors.initials: AP
authors.lastname: dos Santos
authors.forename: Joanne Belle
authors.initials: JB
authors.lastname: Messick
authors.forename: Simone Tostes
authors.initials: ST
authors.lastname: Oliveira
authors.forename: Alexander Welker
authors.initials: AW
authors.lastname: Biondo
authors.forename: Rafael Felipe da Costa
authors.initials: RF
authors.lastname: Vieira
authors.forename: Magnus Larruscaim
authors.initials: ML
authors.lastname: Dalmolin
authors.forename: Félix Hilario Diaz
authors.initials: FH
authors.lastname: González
mesh_terms: D000709:Anaplasma / Q000235:genetics* / Q000302:isolation & purification*; D000818:Animals; D001938:Brazil; D004269:DNA, Bacterial / Q000097:blood*; D004285:Dogs / Q000097:blood*; D041061:Ehrlichia canis / Q000235:genetics* / Q000302:isolation & purification*; D005260:Female; D008297:Male
chemical_list: D004269:DNA, Bacterial