title: Effect of initial protein concentration and pH on in vitro gastric digestion of heated whey proteins.
abstract: The in vitro digestion of heated whey protein aggregates having different structure and physicochemical properties was evaluated under simulated gastric conditions. Aggregates were formed by heating whey protein isolates (WPI) at 3-9% w/w initial protein concentration and pH 3.0-7.0. Results showed that high protein concentration led to formation of larger WPI aggregates with fewer remaining monomers. Aggregates formed at high protein concentrations showed slower degradation rate compared to those formed at low protein concentration. The effect of initial protein concentration on peptide release pattern was not apparent. Heating pH was a significant factor affecting digestion pattern. At pH above the isoelectric point, the majority of the proteins involved in the aggregation, and aggregates formed at pH 6.0 were more susceptible to pepsin digestion than at pH 7.0. At acidic conditions, only small amount of proteins was involved in the aggregation and heated aggregates were easily digested by pepsin, while the remaining unaggregated proteins were very resistant to gastric digestion. The potential physiological implication of these results on satiety was discussed.
authors.affiliation: Food Science Program, Division of Food Systems and Bioengineering, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Sha
authors.initials: S
authors.lastname: Zhang
authors.forename: Bongkosh
authors.initials: B
authors.lastname: Vardhanabhuti
mesh_terms: D004063:Digestion / Q000502:physiology; D005750:Gastric Juice / Q000737:chemistry / Q000378:metabolism; D006361:Heating; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008894:Milk Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D010434:Pepsin A / Q000737:chemistry* / Q000378:metabolism*; D000067816:Whey Proteins
chemical_list: D008894:Milk Proteins; D000067816:Whey Proteins; D010434:Pepsin A
keywords: Aggregates; Digestion; Heat; In vitro; Whey protein