title: The transcriptional and functional properties of mouse epiblast stem cells resemble the anterior primitive streak.
abstract: Mouse epiblast stem cells (EpiSCs) can be derived from a wide range of developmental stages. To characterize and compare EpiSCs with different origins, we derived a series of EpiSC lines from pregastrula stage to late-bud-stage mouse embryos. We found that the transcriptomes of these cells are hierarchically distinct from those of the embryonic stem cells, induced pluripotent stem cells (iPSCs), and epiblast/ectoderm. The EpiSCs display globally similar gene expression profiles irrespective of the original developmental stage of the source tissue. They are developmentally similar to the ectoderm of the late-gastrula-stage embryo and behave like anterior primitive streak cells when differentiated in vitro and in vivo. The EpiSC lines that we derived can also be categorized based on a correlation between gene expression signature and predisposition to differentiate into particular germ-layer derivatives. Our findings therefore highlight distinct identifying characteristics of EpiSCs and provide a foundation for further examination of EpiSC properties and potential.
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia.
authors.forename: Yoji
authors.initials: Y
authors.lastname: Kojima
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia.
authors.forename: Keren
authors.initials: K
authors.lastname: Kaufman-Francis
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia.
authors.forename: Joshua B
authors.initials: JB
authors.lastname: Studdert
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia.
authors.forename: Kirsten A
authors.initials: KA
authors.lastname: Steiner
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia.
authors.forename: Melinda D
authors.initials: MD
authors.lastname: Power
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia; Sydney Medical School, University of Sydney, NSW 2006, Australia.
authors.forename: David A F
authors.initials: DA
authors.lastname: Loebel
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Jones
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia.
authors.forename: Angelyn
authors.initials: A
authors.lastname: Hor
authors.affiliation: Gene Therapy Research Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia.
authors.forename: Gustavo
authors.initials: G
authors.lastname: de Alencastro
authors.affiliation: Gene Therapy Research Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia.
authors.forename: Grant J
authors.initials: GJ
authors.lastname: Logan
authors.affiliation: Bioinformatics Group, Children's Medical Research Institute, Westmead NSW 2145, Australia.
authors.forename: Erdahl T
authors.initials: ET
authors.lastname: Teber
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia.
authors.forename: Oliver H
authors.initials: OH
authors.lastname: Tam
authors.affiliation: Cancer Research Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Stutz
authors.affiliation: Gene Therapy Research Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia; Sydney Medical School, University of Sydney, NSW 2006, Australia.
authors.forename: Ian E
authors.initials: IE
authors.lastname: Alexander
authors.affiliation: Cancer Research Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia; Sydney Medical School, University of Sydney, NSW 2006, Australia.
authors.forename: Hilda A
authors.initials: HA
authors.lastname: Pickett
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia; Sydney Medical School, University of Sydney, NSW 2006, Australia. Electronic address: ptam@cmri.org.au.
authors.forename: Patrick P L
authors.initials: PP
authors.lastname: Tam
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology* / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D054262:Gastrulation; D020869:Gene Expression Profiling; D005855:Germ Layers / Q000166:cytology* / Q000378:metabolism; D007124:Immunoenzyme Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D054240:Primitive Streak / Q000166:cytology* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger