title: Synthesis and hepatoprotective properties ofAcanthus ilicifoliusalkaloid A and its derivatives.
abstract: Acanthus ilicifoliusalkaloid A (4-hydroxy-2(3H)benzoxazolone, HBOA) is a naturally occurring compound that has been separated fromAcanthus ilicifolius. Previous studies have reported the beneficial effects of HBOA on HSC-T6 cells. This study was undertaken in order to synthesize HBOA and two of its derivatives, specifically, 4-acetoxy-2(3H)-benzoxazolone (AcO-BOA) and 3-acetyl-4-acetoxy-2-benzoxazolone (TC-3), and to investigate the hepatoprotective potentials of these three compounds on CCl4-induced liver injury in mice. HBOA was prepared from 2-nitroresorcinol by a 'one pot' reduction and subsequent cyclization with urea. The acyl derivatives, AcO-BOA and TC-3, were prepared from HBOA using a substitution reaction. The compounds were synthesized with good yields (63.08-68.22%). An acute liver injury model was established by administering CCl4intraperitoneally to Kunming mice. The mice were then intragastrically administered bifendate (150 mg/kg) or the synthesized compounds at three different doses (200, 100 and 50 mg/kg). The treatment with CCl4was observed to increase the levels of aminotransferase (ALT), aspartate aminotransferase (AST), lactic dehydrogenase (LDH) and malondialdehyde (MDA) and decrease the levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione peroxidase (Gpx) in the liver tissues of the mice. Furthermore, treatment with CCl4elevated the expression level of the proinflammatory mediator TNF-α. However, HBOA and its derivatives attenuated the changes induced by CCl4. Furthermore, CCl4-induced histopathological changes were reduced by treatment with these compounds. These results suggest that HBOA and its acyl derivatives are able to significantly alleviate the hepatotoxicity induced by CCl4in mice.
authors.affiliation: Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Fan
authors.forename: Ping
authors.initials: P
authors.lastname: Qi
authors.forename: Yan
authors.initials: Y
authors.lastname: Mei
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhou
authors.forename: Liping
authors.initials: L
authors.lastname: Cai
authors.forename: Xing
authors.initials: X
authors.lastname: Lin
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
keywords: Acauthus ilicifolius alkaloid A; acute liver injury; hepatoprotective potential; synthesis