title: Two fluorogenic substrates for purine nucleoside phosphorylase, selective for mammalian and bacterial forms of the enzyme.
abstract: Two nontypical nucleosides, 7-β-D-ribosyl-2,6-diamino-8-azapurine and 8-β-D-ribosyl-2,6-diamino-8-azapurine, have been found to exhibit moderately good, and selective, substrate properties toward calf and bacterial (Escherichia coli) forms of purine nucleoside phosphorylase (PNP). The former compound is effectively phosphorolysed by calf PNP and the latter by PNP from E. coli. Both compounds are fluorescent with λ(max) ∼ 425 to 430 nm, but the reaction product, 2,6-diamino-8-azapurine, emits in a different spectral region (λ(max) ∼ 363 nm) with nearly 40% yield, providing a strong fluorogenic effect at 350 to 360 nm.
authors.affiliation: Department of Biophysics, University of Varmia and Masuria, 10-719 Olsztyn, Poland. Electronic address: jacek.wie@uwm.edu.pl.
authors.forename: Jacek
authors.initials: J
authors.lastname: Wierzchowski
authors.affiliation: Department of Biophysics, University of Varmia and Masuria, 10-719 Olsztyn, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Stachelska-Wierzchowska
authors.affiliation: Division of Biophysics, Institute of Experimental Physics, University of Warsaw, 02-089 Warsaw, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Wielgus-Kutrowska
authors.affiliation: Division of Physical Chemistry, Rudjer Bošković Institute, HR-10002 Zagreb, Croatia.
authors.forename: Goran
authors.initials: G
authors.lastname: Mikleušević
mesh_terms: D000818:Animals; D002415:Cats; D004926:Escherichia coli / Q000201:enzymology*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism*; D011687:Purines / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity
chemical_list: D005456:Fluorescent Dyes; D011687:Purines; D011683:Purine-Nucleoside Phosphorylase
keywords: 8-Azapurines; Fluorogenic substrates; Nucleoside analogs; Purine nucleoside phosphorylase