title: Intragenic DNA methylation status down-regulates bovine IGF2 gene expression in different developmental stages.
abstract: DNA methylation is a key epigenetic modification in mammals and has an essential and important role in muscle development. Insulin-like growth factor 2 (IGF2) is a fetal growth and differentiation factor that plays an important role in muscle growth and in myoblast proliferation and differentiation. The aim of this study was to evaluate the expression of IGF2 and the methylation pattern on the differentially methylated region (DMR) of the last exon of IGF2 in six tissues with two different developmental stages. The DNA methylation pattern was compared using bisulfite sequencing polymerase chain reaction (BSP) and combined bisulfite restriction analysis (COBRA). The quantitative real-time PCR (qPCR) analysis indicated that IGF2 has a broad tissue distribution and the adult bovine group showed significant lower mRNA expression levels than that in the fetal bovine group (P<0.05 or P<0.01). Moreover, the DNA methylation level analysis showed that the adult bovine group exhibited a significantly higher DNA methylation levels than that in the fetal bovine group (P<0.05 or P<0.01). These results indicate that IGF2 expression levels were negatively associated with the methylation status of the IGF2 DMR during the two developmental stages. Our results suggest that the methylation pattern in this DMR may be a useful parameter to investigate as a marker-assisted selection for muscle developmental in beef cattle breeding program and as a model for studies in other species.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling Shaanxi, China.
authors.forename: Yong-Zhen
authors.initials: YZ
authors.lastname: Huang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling Shaanxi, China.
authors.forename: Zhao-Yang
authors.initials: ZY
authors.lastname: Zhan
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling Shaanxi, China.
authors.forename: Yu-Jia
authors.initials: YJ
authors.lastname: Sun
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling Shaanxi, China.
authors.forename: Xiu-Kai
authors.initials: XK
authors.lastname: Cao
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling Shaanxi, China.
authors.forename: Ming-Xun
authors.initials: MX
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling Shaanxi, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling Shaanxi, China.
authors.forename: Xian-Yong
authors.initials: XY
authors.lastname: Lan
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling Shaanxi, China.
authors.forename: Chu-Zhao
authors.initials: CZ
authors.lastname: Lei
authors.affiliation: Institute of Cellular and Molecular Biology, Jiangsu Normal University, Xuzhou Jiangsu, China.
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling Shaanxi, China. Electronic address: chenhong1212@263.net.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002417:Cattle; D019175:DNA Methylation; D015536:Down-Regulation; D005091:Exons; D015870:Gene Expression; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D024510:Muscle Development / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D014018:Tissue Distribution
chemical_list: D012333:RNA, Messenger; D007335:Insulin-Like Growth Factor II
keywords: 3’-UTRs; AB; ANOVA; BSP; Bisulfite sequencing PCR; CCCTC binding factor; COBRA; CTCF; Cattle; Complementary DNA; CpG; Cyt osineguan inedinucleotide; DMR; DNA methylation; FB; H19; IGF2; Insulin-like growth factor 2; LDM; QTLs; QUMA; SEM; SPSS; TSS; TTS; adult bovine; analysis of variance; base pair(s); bp; cDNA; combined bisulfite restriction analysis; differentially methylated region; fetal bovine; histocompatibility gene; longissimus dorsi muscle; miRNA; microRNA; qPCR; quantification tool for methylation analysis; quantitative real-time PCR; quantitative trait locus; regulation at the 3’ untranslated regions; standard error; statistical product and service solutions; the transcription termination site; transcription start site