title: Tumor necrosis factor-α (TNF-α) -863C/A promoter polymorphism is associated with type 2 diabetes in Tunisian population.
abstract: OBJECTIVE
  Tumor necrosis factor α (TNFα) plays a key role in orchestrating the complex events involved in inflammation and immunity. Accordingly, TNF α has been implicated in a wide range of autoimmune and infectious diseases, but also in conditions such as obesity and insulin resistance. The aim of the present study was to investigate the association between the -863C/A polymorphism in the promoter of the TNFα gene and type 2 diabetes in the Tunisian population.
  
  
  METHODS
  The polymorphism -863C/A in the TNFα gene was determined in 211 type 2 diabetes patients and 345 healthy controls using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis.
  
  
  RESULTS
  A significant difference in genotype distribution and allele frequency was observed between patients and controls. Patients with type 2 diabetes had significantly higher frequency of the CA+AA genotypes compared to controls [35.5% vs. 22.3%; OR (95%CI), 1.91 (1.31-2.8); p=0.001]. The type 2 diabetes patient group showed a significant higher frequency of the A allele compared to the controls (0.19 vs. 0.11; p=0.001). After adjustment by a stepwise logistic regression method, hypertension, dyslipidemia, and CA+AA genotype were found to be significantly associated with T2D.
  
  
  CONCLUSIONS
  The present study showed a significant and independent association between the -863C/A polymorphism of the TNFα gene and type 2 diabetes in the Tunisian population.
authors.affiliation: University of Tunis El Manar, la Rabta Hospital, LR99ES11 "Biochimie Clinique", 1007 Tunis, Tunisia.
authors.forename: Amani
authors.initials: A
authors.lastname: Kallel
authors.forename: Bochra
authors.initials: B
authors.lastname: Ftouhi
authors.forename: Zeineb
authors.initials: Z
authors.lastname: Jemaa
authors.forename: Imen
authors.initials: I
authors.lastname: Mahjoubi
authors.forename: Moncef
authors.initials: M
authors.lastname: Feki
authors.forename: Hedia
authors.initials: H
authors.lastname: Slimane
authors.forename: Riadh
authors.initials: R
authors.lastname: Jemaa
authors.forename: Naziha
authors.initials: N
authors.lastname: Kaabachi
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000378:metabolism; D014416:Tunisia / Q000453:epidemiology
chemical_list: D014409:Tumor Necrosis Factor-alpha
keywords: Single nucleotide polymorphism; TNFα; Type 2 diabetes