title: Formation of targeted monovalent quantum dots by steric exclusion.
abstract: Precise control over interfacial chemistry between nanoparticles and other materials remains a major challenge that limits broad application of nanotechnology in biology. To address this challenge, we used 'steric exclusion' to completely convert commercial quantum dots (QDs) into monovalent imaging probes by wrapping each QD with a functionalized oligonucleotide. We demonstrated the utility of these QDs as modular and nonperturbing imaging probes by tracking individual Notch receptors on live cells.
authors.affiliation: 1] Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, USA. [2] Tetrad Graduate Program, University of California San Francisco, San Francisco, California, USA. [3] Center for Systems and Synthetic Biology, University of California San Francisco, San Francisco, California, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Farlow
authors.forename: Daeha
authors.initials: D
authors.lastname: Seo
authors.forename: Kyle E
authors.initials: KE
authors.lastname: Broaders
authors.forename: Marcus J
authors.initials: MJ
authors.lastname: Taylor
authors.forename: Zev J
authors.initials: ZJ
authors.lastname: Gartner
authors.forename: Young-Wook
authors.initials: YW
authors.lastname: Jun
mesh_terms: D045744:Cell Line, Tumor; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells; D008027:Light; D008051:Lipid Bilayers / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry; D046529:Microscopy, Electron, Transmission / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry; D054735:Phosphorothioate Oligonucleotides / Q000737:chemistry; D016012:Poisson Distribution; D045663:Quantum Dots; D012542:Scattering, Radiation; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008565:Membrane Proteins; D009841:Oligonucleotides; D054735:Phosphorothioate Oligonucleotides; D013438:Sulfhydryl Compounds; D049452:Green Fluorescent Proteins