title: Volatile analysis and antimicrobial screening of the parasitic plant Cuscuta reflexa Roxb. from Nepal.
abstract: The essential oil from the parasitic vine Cuscuta reflexa Roxb., collected from Kirtipur, Kathmandu, Nepal, was obtained by hydrodistillation and analysed by gas chromatography-mass spectrometry. From a total of 62 peaks, 61 compounds were identified in the oil, accounting for 99.6% of the oil. The majority of the essential oil was dominated by the relatively rare component cis-3-butyl-4-vinylcyclopentane (26.4%). The oil also contained substantial amounts of limonene (5.1%) and (E)-nerolidol (9.5%). Biological screening for antimicrobial activities did not show appreciable activity against either Gram-positive (Bacillus cereus and Staphylococcus aureus) or Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria. However, marginal activity against Aspergillus niger was observed (minimum inhibitory concentration = 313 μg/mL).
authors.affiliation: a Department of Chemistry , University of Alabama in Huntsville , Huntsville , AL 35899 , USA.
authors.forename: Prajwal
authors.initials: P
authors.lastname: Paudel
authors.forename: Prabodh
authors.initials: P
authors.lastname: Satyal
authors.forename: Samjhana
authors.initials: S
authors.lastname: Maharjan
authors.forename: Nawal
authors.initials: N
authors.lastname: Shrestha
authors.forename: William N
authors.initials: WN
authors.lastname: Setzer
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001234:Aspergillus niger / Q000187:drug effects; D034182:Cuscuta / Q000737:chemistry*; D053138:Cyclohexenes / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D020128:Inhibitory Concentration 50; D000077222:Limonene; D008826:Microbial Sensitivity Tests; D009390:Nepal; D009822:Oils, Volatile / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012717:Sesquiterpenes / Q000032:analysis; D013211:Staphylococcus aureus / Q000187:drug effects; D013729:Terpenes / Q000032:analysis
chemical_list: D000935:Antifungal Agents; D053138:Cyclohexenes; D009822:Oils, Volatile; D012717:Sesquiterpenes; D013729:Terpenes; D000077222:Limonene; C037055:nerolidol