title: Evaluation of agar-based medium with sheep sera for testing of drug susceptibility of Mycobacterium tuberculosis to Isoniazid, Rifampin, Ethambutol, and Streptomycin.
abstract: The performance of sheep sera instead of sheep blood in agar-based media was investigated for susceptibility testing of Mycobacterium tuberculosis against primary drugs. The levels of agreement between agar-based medium supplemented with sheep sera and the proportion method on Middlebrook 7H11 agar as the reference method for determining susceptibility to isoniazid (INH), rifampin (RIF), ethambutol (EMB), and streptomycin (STR) were 98.4, 98.4, 95.3, and 100%, respectively.
authors.affiliation: Ondokuz Mayis University, Medical School, Department of Medical Microbiology, Samsun, Turkey.
authors.forename: Ahmet Yilmaz
authors.initials: AY
authors.lastname: Coban
authors.forename: Meltem
authors.initials: M
authors.lastname: Uzun
authors.forename: Bulent
authors.initials: B
authors.lastname: Bozdogan
mesh_terms: D000362:Agar; D000818:Animals; D000995:Antitubercular Agents / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D044967:Serum; D012756:Sheep
chemical_list: D000995:Antitubercular Agents; D003470:Culture Media; D000362:Agar