title: Novel inhibitory function of miR-125b in melanogenesis.
abstract: MicroRNAs are known to be the important regulators of skin physiology and considered as new therapeutic targets to treat skin diseases. In this study, miR-125b was identified as a potent regulator of steady-state melanogenesis. We found that the expression of miR-125b was inversely related to pigment levels. A miR-125b mimic decreased the expression of pigmentation-related gene and melanin content, implying that miR-125b functions to decrease pigmentation. Moreover, we observed that the reduction in miR-125b expression in pigmented cells was at least partially due to the hypermethylation of the MIR125B-1 promoter, and miR-125b expression was regulated by intracellular cAMP levels.
authors.affiliation: Bioscience Research Division, R&D Unit, AmorePacific Corporation, Yongin-si, Korea.
authors.forename: Kyu-Han
authors.initials: KH
authors.lastname: Kim
authors.forename: Bum-Ho
authors.initials: BH
authors.lastname: Bin
authors.forename: Juewon
authors.initials: J
authors.lastname: Kim
authors.forename: Son Eui
authors.initials: SE
authors.lastname: Dong
authors.forename: Phil June
authors.initials: PJ
authors.lastname: Park
authors.forename: Hyunjung
authors.initials: H
authors.lastname: Choi
authors.forename: Beom Joon
authors.initials: BJ
authors.lastname: Kim
authors.forename: Seok Jong
authors.initials: SJ
authors.lastname: Yu
authors.forename: Hyojin
authors.initials: H
authors.lastname: Kang
authors.forename: Hak Hee
authors.initials: HH
authors.lastname: Kang
authors.forename: Eun-Gyung
authors.initials: EG
authors.lastname: Cho
authors.forename: Tae Ryong
authors.initials: TR
authors.lastname: Lee
mesh_terms: D002460:Cell Line; D000242:Cyclic AMP / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D008543:Melanins / Q000096:biosynthesis* / Q000235:genetics; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D010858:Pigmentation / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000502:physiology*
chemical_list: C582445:MIRN1260 microRNA, human; D008543:Melanins; D035683:MicroRNAs; D000242:Cyclic AMP
keywords: melanogenesis; melanoma; miR-125b; microRNA; steady-state