title: Diversity among strains of Pseudomonas aeruginosa from manure and soil, evaluated by multiple locus variable number tandem repeat analysis and antibiotic resistance profiles.
abstract: The results of a multiple locus variable number of tandem repeat (VNTR) analysis (MLVA)-based study designed to understand the genetic diversity of soil and manure-borne Pseudomonas aeruginosa isolates, and the relationship between these isolates and a set of clinical and environmental isolates, are hereby reported. Fifteen described VNTR markers were first selected, and 62 isolates recovered from agricultural and industrial soils in France and Burkina Faso, and from cattle and horse manure, along with 26 snake-related isolates and 17 environmental and clinical isolates from international collections, were genotyped. Following a comparison with previously published 9-marker MLVA schemes, an optimal 13-marker MLVA scheme (MLVA13-Lyon) was identified that was found to be the most efficient, as it showed high typability (90%) and high discriminatory power (0.987). A comparison of MLVA with PFGE for typing of the snake-related isolates confirmed the MLVA13-Lyon scheme to be a robust method for quickly discriminating and inferring genetic relatedness among environmental isolates. The 62 isolates displayed wide diversity, since 41 MLVA types (i.e. MTs) were observed, with 26 MTs clustered in 10 MLVA clonal complexes (MCs). Three and eight MCs were found among soil and manure isolates, respectively. Only one MC contained both soil and manure-borne isolates. No common MC was observed between soil and manure-borne isolates and the snake-related or environmental and clinical isolates. Antibiotic resistance profiles were performed to determine a potential link between resistance properties and the selective pressure that might be present in the various habitats. Except for four soil and manure isolates resistant to ticarcillin and ticarcillin/clavulanic acid and one isolate from a hydrocarbon-contaminated soil resistant to imipenem, all environmental isolates showed wild-type antibiotic profiles.
authors.affiliation: Université de Lyon, Research Group on "Environmental Multiresistance and Efflux Pumps", CNRS, Ecole nationale vétérinaire de Lyon, and Université Lyon 1, UMR 5557 Ecologie microbienne, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne cedex, France. Electronic address: benjamin.youenou@gmail.com.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Youenou
authors.affiliation: Université de Lyon, Research Group on "Environmental Multiresistance and Efflux Pumps", CNRS, Ecole nationale vétérinaire de Lyon, and Université Lyon 1, UMR 5557 Ecologie microbienne, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne cedex, France.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Brothier
authors.affiliation: Université de Lyon, Research Group on "Environmental Multiresistance and Efflux Pumps", CNRS, Ecole nationale vétérinaire de Lyon, and Université Lyon 1, UMR 5557 Ecologie microbienne, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne cedex, France. Electronic address: sylvie.nazaret@univ-lyon1.fr.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Nazaret
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D024881:Drug Resistance, Bacterial; D005819:Genetic Markers; D014644:Genetic Variation; D008372:Manure / Q000382:microbiology*; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects* / Q000235:genetics*; D016415:Sequence Alignment; D012988:Soil Microbiology
chemical_list: D000900:Anti-Bacterial Agents; D005819:Genetic Markers; D008372:Manure
keywords: Antibiotics; MLVA typing; Pseudomonas aeruginosa; Resistance