title: [Role of microRNA-141 in the regulation of synthesis of high mobility group protein B1 in THP-1 cells].
abstract: OBJECTIVE
  To investigate the regulatory effect of microRNA-141 (miR-141) on expression of high mobility group protein B1(HMGB1) in human monocytes THP-1 cell line.
  
  
  METHODS
  THP-1 cells were transfected with miR-141 mimic or inhibitor (100 nmol/L) for 48 hours with lipofectamine RNAi MAX. The levels of miR-141 and HMGB1 mRNA in the THP-1 cells were detected by real-time fluorescence quantitation reverse transcription-polymerase chain reaction (RT-PCR), and HMGB1 protein was determined with Western blotting.
  
  
  RESULTS
  The levels of miR-141 could be up regulated (35.33±7.24 vs. 1.21±0.20, t=-8.408, P=0.010) or down regulated (0.55±0.12 vs 1.09±0.05, t=7.473, P=0.002) after being transfected with 100 nmol/L miR-141 mimic or inhibitor for 48 hours by lipofectamine RNAi MAX in THP-1, and the level of HMGB1 mRNA and protein decreased (mRNA: 0.43±0.06 vs. 0.97±0.08, t=9.760, P=0.001; protein: 0.63±0.12 vs. 1.00±0.11, t=2.991, P=0.040) or increased (mRNA: 2.13±0.11 vs. 1.16±0.13, t=-9.977, P=0.001; protein: 1.78±0.04 vs. 0.96±0.09, t=-13.778, P=0.000) simultaneously compared with the control group.
  
  
  CONCLUSIONS
  miR-141 is involved in regulation of inflammation through HMGB1 gene and protein pathway, suggesting that miR-141 plays an important role in regulating immune cells during the inflammatory response.
authors.affiliation: Department of Critical Care Medicine, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, Guangdong, China, Corresponding author: Xiong Xu-ming, Email: xiongxuming9@126.com.
authors.forename: Zhen-hui
authors.initials: ZH
authors.lastname: Zhang
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Chen
authors.forename: Zi-xin
authors.initials: ZX
authors.lastname: Jiang
authors.forename: Wei-yan
authors.initials: WY
authors.lastname: Chen
authors.forename: Xu-ming
authors.initials: XM
authors.lastname: Xiong
mesh_terms: D002460:Cell Line; D024243:HMGB1 Protein / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D014162:Transfection
chemical_list: D024243:HMGB1 Protein; C585491:HMGB1 protein, human; C533895:MIRN141 microRNA, human; D035683:MicroRNAs