title: Zinc finger X-chromosomal protein (ZFX) promotes solid agar colony growth of osteosarcoma cells.
abstract: Zinc finger X-chromosomal protein (ZFX) is a member of the zinc finger family of proteins. The importance of ZFX in several cancer types, including prostate cancer, laryngeal squamous cell carcinoma, and glioma, has been addressed. However, the role of ZFX in human osteosarcoma remains unknown. Here we investigated the phenotype of ZFX knockdown on cell proliferation and in vitro tumorigenesis using lentivirus-mediated loss-of-function strategy. The results demonstrated that the proliferation and colony formation ability of human osteosarcoma Saos-2 and MG63 cells was impaired by ZFX small interfering RNA (siRNA)-expressing lentivirus. Moreover, loss of ZFX led to G0/G1 phase cell cycle arrest and a significant increase of cells in the sub-G1 fraction, indicating that ZFX functions as an oncogene in the malignant proliferation process in osteosarcoma. Furthermore, ZFX siRNA may have an antitumorigenic effect on osteosarcoma cells. Our findings hold important significance for RNA interference-mediated cancer gene therapy for human osteosarcoma.
authors.affiliation: Department of Orthopedic Surgery, China-Japan Union Hospital of Jilin University, Changchun, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Jiang
authors.forename: Jin-cheng
authors.initials: JC
authors.lastname: Wang
authors.forename: Mei
authors.initials: M
authors.lastname: Sun
authors.forename: Xing-yi
authors.initials: XY
authors.lastname: Zhang
authors.forename: Han
authors.initials: H
authors.lastname: Wu
mesh_terms: D000362:Agar; D017209:Apoptosis / Q000502:physiology; D001859:Bone Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D063646:Carcinogenesis / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D018929:Cell Culture Techniques; D048708:Cell Growth Processes / Q000502:physiology; D015536:Down-Regulation; D055785:Gene Knockdown Techniques; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D016335:Zinc Fingers
chemical_list: D051741:Kruppel-Like Transcription Factors; D034741:RNA, Small Interfering; C062076:zinc finger protein, X-linked; D000362:Agar