title: Effect of mesenchymal stem cells and extracts derived from the placenta on trophoblast invasion and immune responses.
abstract: Tightly regulated trophoblast invasion and immunomodulation at the feto-maternal interface is important during implantation and fetal development. Although trophoblasts as a pregnancy-specific cell has been reported to be a key factor capable of regulating certain events during implantation, however, its regulatory mechanisms are still unclear. In this study, we analyzed the effects of chorionic plate-derived mesenchymal stem cells (CP-MSCs) and human placenta extract (hPE) isolated from human normal placentas on trophoblasts invasion and immune responses. We investigated the effects of CP-MSCs, hPE treatment, and their combination on trophoblasts invasion and on T-cells suppression through human leukocyte antigen-G (HLA-G) expression. Trophoblasts invasion was significantly increased by co-culture of CP-MSCs or by hPE treatment (P<0.05), and enhanced by the combination of CP-MSCs and hPE treatment (P<0.05). The proliferation of T-cells was decreased by co-culture of CP-MSCs and hPE treatment, whereas the population of regulatory T-cells was increased (P<0.05). Also, the dynamics alterations of multiple cytokines were observed in the culture supernatants of trophoblasts and T-cells depending on CP-MSCs co-culture and hPE treatment. Interestingly, the concentration of soluble HLA-G was increased by CP-MSCs co-culture, by hPE treatment and by combination of them on trophoblasts and activated T-cells (P<0.05). These findings suggested that CP-MSCs and hPE can regulate trophoblasts invasion and T-cell by alteration of HLA-G expression. These results will provide understandings of trophoblasts invasion and the immunological network at the feto-maternal interface during pregnancy and contribute to the foundation of a new treatment strategy for pregnancy disorders.
authors.affiliation: 1 Department of Biomedical Science, CHA University , Seoul, Republic of Korea.
authors.forename: Jong Ho
authors.initials: JH
authors.lastname: Choi
authors.forename: Jieun
authors.initials: J
authors.lastname: Jung
authors.forename: Kyu-Hwan
authors.initials: KH
authors.lastname: Na
authors.forename: Kyung Jin
authors.initials: KJ
authors.lastname: Cho
authors.forename: Tae Ki
authors.initials: TK
authors.lastname: Yoon
authors.forename: Gi Jin
authors.initials: GJ
authors.lastname: Kim
mesh_terms: D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D010064:Embryo Implantation / Q000276:immunology; D005260:Female; D059951:HLA-G Antigens / Q000097:blood; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000276:immunology*; D010920:Placenta / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014327:Trophoblasts / Q000166:cytology* / Q000276:immunology*
chemical_list: D059951:HLA-G Antigens