title: Ganglioside GM1 reduces white matter damage in neonatal rats.
abstract: This study investigated the neuronal protective effect of monosialotetrahexosylganglioside (GM1) on the hypoxia-ischemia white matter damage (WMD) in neonatal rats. Brain hypoxia-ischemia was induced by bilateral carotid artery occlusion in 4-day-old neonatal rats. Bilateral carotid artery occlusion (BCAO) was performed in rats in WMD and GM1 groups, while in sham group; the rat bilateral carotid arteries were merely exposed without occlusion. Immunohistochemical staining was used to determine the expression of myelin basic protein (MBP), glial fibrillary acidic protein (GFAP), and beta-amyloid precursor protein (beta-APP). In addition, suspension test, slope test, and open-field test were carried out on day 26 after BCAO to determine the neurobehavioral function. The percentage of MBP-positive cells was decreased while beta-APP-and GFAPpositive cells were increased in WMD group. After treated with GM1, the percentage of MBP-positive cells increased significantly than WMD rats at post-operation 72 h and day 7. GFAP-positive cells and beta-APP-positive cells decreased significantly in WMD group at post-operation 72 h, day 7 and 26. The suspension test, slope test, and open-field test showed that neurobehavioral function was improved in ganglioside GM1 group compared with WMD group. Taken together, our findings suggested that ganglioside GM1 treatment reduces hypoxia-ischemia induced impairment of the neurobehavioral function in WMD in neonatal rats.
authors.affiliation: Department of Neonatology, Guangzhou Women and Children's Medical Center, Guangzhou Medical College, Guangzhou, China, zhouwei_pu02@126.com.
authors.forename: Xiao
authors.initials: X
authors.lastname: Rong
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Chen
authors.initials: C
authors.lastname: Xiao-Wen
authors.forename: Li
authors.initials: L
authors.lastname: Tao
authors.forename: Juan
authors.initials: J
authors.lastname: Tang
mesh_terms: D016564:Amyloid beta-Protein Precursor / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002340:Carotid Artery Diseases / Q000150:complications*; D004195:Disease Models, Animal; D005260:Female; D005677:G(M1) Ganglioside / Q000627:therapeutic use*; D005786:Gene Expression Regulation / Q000187:drug effects; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D056784:Leukoencephalopathies / Q000188:drug therapy* / Q000209:etiology*; D008297:Male; D004676:Myelin Basic Protein / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D016564:Amyloid beta-Protein Precursor; D005904:Glial Fibrillary Acidic Protein; D004676:Myelin Basic Protein; D005677:G(M1) Ganglioside