title: Subchronic exposure to lead acetate inhibits spermatogenesis and downregulates the expression of Ddx3y in testis of mice.
abstract: Male mice were given drinking water alone or containing 0.5, 1.0 and 1.5g/L Pb acetate for 60 days. After the treatment, the concentrations of Pb were determined in the serum and testis of mice by ICP-MS. The gene transcription of Usp9y, Ddx3y and Uty and their protein levels in the testis were examined by real-time PCR, Western blotting and immunohistochemistry. The sperm quality, spermatogenesis, and histological alteration of the testis and epididymis were observed by microscope. The probability of impregnating female mice by lead-exposed male was evaluated. It was shown that the concentrations of Pb in serum and testis of mice significantly increased in the groups exposed to Pb in a dose-dependent manner. The male fertility significantly decreased in the groups exposed to 1.0 and 1.5g/L Pb acetate. Moreover, exposure to Pb also inhibited spermatogenesis and sperm development, and significantly downregulated expressions of Ddx3y gene expression in testis of mice, but Usp9y and Uty expressions unaffected.
authors.affiliation: Department of Occupational and Environmental Health, Dalian Medical University, Dalian, Liaoning 116044, China.
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Wang
authors.forename: Man
authors.initials: M
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Dong
authors.forename: Yachen
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Zheng
authors.forename: Fengyuan
authors.initials: F
authors.lastname: Piao
authors.forename: Sheng
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D053487:DEAD-box RNA Helicases / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D005298:Fertility / Q000187:drug effects; D008297:Male; D051379:Mice; D015778:Minor Histocompatibility Antigens; D009942:Organometallic Compounds / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D012661:Semen / Q000166:cytology / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D059550:Toxicity Tests, Subchronic
chemical_list: D015778:Minor Histocompatibility Antigens; D009942:Organometallic Compounds; D012333:RNA, Messenger; C115600:Ddx3y protein, mouse; D053487:DEAD-box RNA Helicases; C008261:lead acetate
keywords: Ddx3y; Lead acetate; Male reproductive toxicity; Spermatogenesis; Y chromosome gene