title: miRNA-205 affects infiltration and metastasis of breast cancer.
abstract: BACKGROUND
  An increasing number of studies have shown that miRNAs are commonly deregulated in human malignancies, but little is known about the function of miRNA-205 (miR-205) in human breast cancer. The present study investigated the influence of miR-205 on breast cancer malignancy.
  
  
  METHODS
  The expression level of miR-205 in the MCF7 breast cancer cell line was determined by quantitative (q)RT-PCR. We then analyzed the expression of miR-205 in breast cancer and paired non-tumor tissues. Finally, the roles of miR-205 in regulating tumor proliferation, apoptosis, migration, and target gene expression were studied by MTT assay, flow cytometry, qRT-PCR, Western blotting and luciferase assay.
  
  
  RESULTS
  miR-205 was downregulated in breast cancer cells or tissues compared with normal breast cell lines or non-tumor tissues. Overexpression of miR-205 reduced the growth and colony-formation capacity of MCF7 cells by inducing apoptosis. Overexpression of miR-205 inhibited MCF7 cell migration and invasiveness. By bioinformation analysis, miR-205 was predicted to bind to the 3' untranslated regions of human epidermal growth factor receptor (HER)3 mRNA, and upregulation of miR-205 reduced HER3 protein expression.
  
  
  CONCLUSIONS
  miR-205 is a tumor suppressor in human breast cancer by post-transcriptional inhibition of HER3 expression.
authors.affiliation: Department of Chest Surgery, The First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, China; Department of Tumor, SenGong Hospital of Shaanxi, Xi'an 710300, China.
authors.forename: Zhouquan
authors.initials: Z
authors.lastname: Wang
authors.forename: Hehe
authors.initials: H
authors.lastname: Liao
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Deng
authors.forename: Po
authors.initials: P
authors.lastname: Yang
authors.forename: Ning
authors.initials: N
authors.lastname: Du
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Zhanng
authors.forename: Hong
authors.initials: H
authors.lastname: Ren
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D020893:Receptor, ErbB-3 / Q000378:metabolism; D014410:Tumor Stem Cell Assay
chemical_list: D035683:MicroRNAs; D020893:Receptor, ErbB-3; C534425:MIR205, human; C581292:ERBB3 protein, human
keywords: Breast cancer; Tumor suppressor; miRNA-205; miRNAs