title: Yeast three-hybrid screening for identifying anti-tuberculosis drug targets.
abstract: Mycobacterium goes yeast: Target deconvolution of anti-tuberculosis drugs can be a very challenging task. Here we report a yeast 3-hybrid system that allows promising small molecules to be screened for protein targets of a pathogen in nontoxic yeast cells. The system employs libraries of randomly fragmented bacterial DNA and offers a technically simple alternative approach for target identification.
authors.affiliation: Institute of Chemical Science and Engineering, Institute of Bioengineering, National Centre of Competence in Research NCCR Chemical Biology, École Polytechnique Fédérale de Lausanne, EPFL SB ISIC LIP, 1015 Lausanne (Switzerland); Current address: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139 (USA).
authors.forename: Simone
authors.initials: S
authors.lastname: Moser
authors.forename: Kai
authors.initials: K
authors.lastname: Johnsson
mesh_terms: D000995:Antitubercular Agents / Q000737:chemistry / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D015394:Molecular Structure; D058990:Molecular Targeted Therapy / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D019151:Peptide Library; D054852:Small Molecule Libraries / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D020798:Two-Hybrid System Techniques
chemical_list: D000995:Antitubercular Agents; D004269:DNA, Bacterial; D019151:Peptide Library; D054852:Small Molecule Libraries
keywords: genomic library; snap-tag; target deconvolution; tuberculosis; yeast three-hybrid system