title: Mass spectrometry-based imaging of metabolites and proteins.
abstract: Imaging techniques based on mass spectrometry (MS) have become powerful approaches to decipher the spatial distribution of metabolites and proteins. MS imaging (MSI) mostly relies on matrix-assisted laser desorption/ionization coupled to MS detection, but desorption electrospray ionization is also frequently used. Here we describe our current protocols for MALDI-MSI of seed sections and for root tissue. Detailed procedures for cryo-sectioning, matrix application, image capture, mass spectrometry measurement and data analysis are given.
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
authors.forename: Manuela
authors.initials: M
authors.lastname: Peukert
authors.forename: Michael
authors.initials: M
authors.lastname: Becker
authors.forename: Andrea
authors.initials: A
authors.lastname: Matros
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Mock
mesh_terms: D001467:Hordeum / Q000378:metabolism*; D021621:Imaging, Three-Dimensional; D055442:Metabolome; D010455:Peptides / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D025341:Principal Component Analysis; D012639:Seeds / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014026:Nicotiana / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism
chemical_list: D010455:Peptides; D010940:Plant Proteins; D014357:Trypsin