title: Scalable production of adenovirus vectors.
abstract: Recombinant adenoviruses (AdV) are highly efficient at gene transfer for a broad spectrum of cell types and species. They became one of the vectors of choice for gene delivery and expression of foreign proteins in gene therapy and vaccination purposes. To meet the need of significant amounts of adenoviral vectors for preclinical and possibly clinical uses, scalable and reproducible production processes are required.In this chapter, we review processes used for scalable production of two types of first generation (E1-deleted) adenoviral vectors (Human and Canine) using stirred tank bioreactors. The production of adenovirus vectors using either suspension (HEK 293) or anchorage-dependent cells (MDCK-E1) are described to exemplify scalable production processes with different cell-culture types. The downstream processes will be covered in the next chapter.
authors.affiliation: Animal Cell Technology Unit, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa (ITQB-UNL), Oeiras, Portugal.
authors.forename: Ana Carina
authors.initials: AC
authors.lastname: Silva
authors.forename: Paulo
authors.initials: P
authors.lastname: Fernandes
authors.forename: Marcos F Q
authors.initials: MF
authors.lastname: Sousa
authors.forename: Paula M
authors.initials: PM
authors.lastname: Alves
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000302:isolation & purification*; D006516:Adenoviruses, Canine / Q000235:genetics / Q000302:isolation & purification; D000260:Adenoviruses, Human / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D061249:Batch Cell Culture Techniques / Q000379:methods*; D019149:Bioreactors; D002460:Cell Line; D005822:Genetic Vectors / Q000235:genetics / Q000302:isolation & purification*; D057809:HEK293 Cells; D006801:Humans; D019562:Viral Load