title: In situ preparation of magnetic Fe3O4 nanoparticles inside nanoporous poly(L-glutamic acid)/chitosan microcapsules for drug delivery.
abstract: The magnetic polymer microcapsules, as a promising environmental stimuli-responsive delivery vehicle, have been increasingly exploited to tackle the problem of remotely navigated delivery. This study presented a novel design and fabrication of magnetic poly(L-glutamic acid)/chitosan (PGA/CS) microcapsules. Magnetic Fe3O4 nanoparticles were in situ synthesized inside nanoporous PGA/CS microcapsules and resultant magnetic PGA/CS microcapsules were characterized. Mitoxantrone (MTX), an antineoplastic drug, was chosen as a water-soluble model drug to research the loading and release properties of the microcapsules. The results showed the carboxylate groups of PGA within polyelectrolyte walls could be used as binding sites for the absorption of iron ions and reaction sites for the synthesis of magnetic nanoparticles. Magnetic PGA/CS microcapsules were dissected using a dual-beam scanning electron microscope/focused ion beam (SEM/FIB) for morphological and microstructural examination. It was found that Fe3O4 nanoparticles with size of about 10nm were homogeneously dispersed in the polymer matrix and adhered to the pore walls of the microcapsules. Increasing the concentration of iron ions led to an increasing loading content of Fe3O4 nanoparticles and an increase in the resultant magnetization. The magnetic PGA/CS microcapsules could be easily manipulated by an external magnetic field. The MTX loading capacity depended on loading time and MTX concentration. The high loading could be ascribed to spontaneous deposition of MTX induced by electrostatic interaction. The microcapsules exhibited sustained release behavior. The MTX release from microcapsules could be best described using Korsmeyer-Peppas and Baker-Lonsdale models, indicating the diffusion mechanism of drug release from both PGA/CS microcapsules and magnetic PGA/CS microcapsules. Therefore, the novel magnetic PGA/CS microcapsules are expected to find application in drug delivery systems because of the properties of magnetic sensitivity, high drug loading and sustained release.
authors.affiliation: Department of Polymer Materials, Shanghai University, Shanghai 200444, China. Electronic address: yansf@staff.shu.edu.cn.
authors.forename: Shifeng
authors.initials: S
authors.lastname: Yan
authors.affiliation: Department of Polymer Materials, Shanghai University, Shanghai 200444, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Polymer Materials, Shanghai University, Shanghai 200444, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Polymer Materials, Shanghai University, Shanghai 200444, China.
authors.forename: Taotao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. Electronic address: xschen@ciac.jl.cn.
authors.forename: Xuesi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Polymer Materials, Shanghai University, Shanghai 200444, China. Electronic address: jbyin@oa.shu.edu.cn.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Yin
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods; D018698:Glutamic Acid / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D011099:Polyglutamic Acid / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
chemical_list: D058185:Magnetite Nanoparticles; D011108:Polymers; D011099:Polyglutamic Acid; D018698:Glutamic Acid; D048271:Chitosan
keywords: Fe(3)O(4) nanoparticles; Magnetic microcapsules; Nanoporous microcapsules