title: Electrophysiological and structural determinants of electrotonic modulation of repolarization by the activation sequence.
abstract: Spatial dispersion of repolarization is known to play an important role in arrhythmogenesis. Electrotonic modulation of repolarization by the activation sequence has been observed in some species and tissue preparations, but to varying extents. Our study sought to determine the mechanisms underlying species- and tissue-dependent electrotonic modulation of repolarization in ventricles. Epi-fluorescence optical imaging of whole rat hearts and pig left ventricular wedges were used to assess epicardial spatial activation and repolarization characteristics. Experiments were supported by computer simulations using realistic geometries. Tight coupling between activation times (AT) and action potential duration (APD) were observed in rat experiments but not in pig. Linear correlation analysis found slopes of -1.03 ± 0.59 and -0.26 ± 0.13 for rat and pig, respectively (p < 0.0001). In rat, maximal dispersion of APD was 11.0 ± 3.1 ms but dispersion of repolarization time (RT) was relatively homogeneous (8.2 ± 2.7, p < 0.0001). However, in pig no such difference was observed between the dispersion of APD and RT (17.8 ± 6.1 vs. 17.7 ± 6.5, respectively). Localized elevations of APD (12.9 ± 8.3%) were identified at ventricular insertion sites of rat hearts both in experiments and simulations. Tissue geometry and action potential (AP) morphology contributed significantly to determining influence of electrotonic modulation. Simulations of a rat AP in a pig geometry decreased the slope of AT and APD relationships by 70.6% whereas slopes were increased by 75.0% when implementing a pig AP in a rat geometry. A modified pig AP, shortened to match the rat APD, showed little coupling between AT and APD with greatly reduced slope compared to the rat AP. Electrotonic modulation of repolarization by the activation sequence is especially pronounced in small hearts with murine-like APs. Tissue architecture and AP morphology play an important role in electrotonic modulation of repolarization.
authors.affiliation: Faculty of Biological Sciences, Multidisciplinary Cardiovascular Research Centre, School of Biomedical Sciences, Institute of Membrane and Systems Biology, University of Leeds Leeds, UK ; Unité Inserm 1045, Centre de Recherche Cardio-Thoracique, Université Bordeaux Segalen Bordeaux, France ; L'Institut de Rythmologie et Modélisation Cardiaque, Université de Bordeaux Bordeaux, France.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Walton
authors.forename: Alan P
authors.initials: AP
authors.lastname: Benson
authors.forename: Matthew E L
authors.initials: ME
authors.lastname: Hardy
authors.forename: Ed
authors.initials: E
authors.lastname: White
authors.forename: Olivier
authors.initials: O
authors.lastname: Bernus
keywords: action potential duration; cardiac electrophysiology; electrotonic current; heterogeneity; ventricular repolarization