title: 16S rRNA PCR followed by restriction endonuclease digestion: a rapid approach for genus level identification of important enteric bacterial pathogens.
abstract: The study describes a rapid approach for detection of common enteric bacterial pathogens, which involves partial amplification of the 16S rRNA gene by PCR using a colony from selective medium followed by restriction enzyme (RE) digestion using the EcoRI, HindIII and SalI enzymes. On the basis of RE digestion analysis different genera namely, Escherichia, Salmonella, Shigella, Vibrio, Campylobacter, Arcobacter, Yesinia and Listeria were differentiated.
authors.affiliation: Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, 243 122, India.
authors.forename: J
authors.initials: J
authors.lastname: Vergis
authors.forename: M
authors.initials: M
authors.lastname: Negi
authors.forename: K
authors.initials: K
authors.lastname: Poharkar
authors.forename: D P
authors.initials: DP
authors.lastname: Das
authors.forename: S V S
authors.initials: SV
authors.lastname: Malik
authors.forename: A
authors.initials: A
authors.lastname: Kumar
authors.forename: S P
authors.initials: SP
authors.lastname: Doijad
authors.forename: S B
authors.initials: SB
authors.lastname: Barbuddhe
authors.forename: D B
authors.initials: DB
authors.lastname: Rawool
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D004262:DNA Restriction Enzymes / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D005767:Gastrointestinal Diseases / Q000175:diagnosis* / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000382:microbiology; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis / Q000382:microbiology; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D004262:DNA Restriction Enzymes
keywords: 16S rRNA; Diarrhea; Enteric bacterial pathogen; RE digestion