title: Effect of dietary eugenol on xenobiotic metabolism and mediation of UDP-glucuronosyltransferase and cytochrome P450 1A1 expression in rat liver.
abstract: Xenobiotic-metabolizing enzymes (XMEs) play an important role in the elimination and detoxification of xenobiotics and drugs. A variety of natural dietary agents are known to protect against cancer by inducing XME. To elucidate the molecular mechanism of XME induction, we examined the effect of dietary eugenol (4-allyl-1-hydroxy-2-methoxybenzene) on xenobiotic metabolism. In this study, rats were administered dietary eugenol for 4 weeks to investigate the various effects of UDP-glucuronosyltransferase (UGT) and cytochrome P450 (CYP) expression. In rats administered dietary eugenol, expression levels of hepatic CYP1A 1 were reduced to 40% than of the controls, while expression of hepatic UGT1A6, UGT1A7 and UGT2B1 increased to 2-3 times than observed in the controls. Hepatic protein levels of UGT1A6 and 2B1 were also elevated in the eugenol-treated rats. These results suggest that the natural compound eugenol improves the xenobiotic-metabolizing systems that suppress and induce the expression of CYP1A1 and UGT, respectively.
authors.affiliation: Laboratory of Veterinary Biochemistry, Graduate School of Veterinary Medicine .
authors.forename: Hidetomo
authors.initials: H
authors.lastname: Iwano
authors.forename: Wakako
authors.initials: W
authors.lastname: Ujita
authors.forename: Miyu
authors.initials: M
authors.lastname: Nishikawa
authors.forename: Satomi
authors.initials: S
authors.lastname: Ishii
authors.forename: Hiroki
authors.initials: H
authors.lastname: Inoue
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yokota
mesh_terms: D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D004032:Diet; D004347:Drug Interactions; D005054:Eugenol / Q000494:pharmacology* / Q000627:therapeutic use; D014453:Glucuronosyltransferase / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D009369:Neoplasms / Q000517:prevention & control; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015262:Xenobiotics / Q000378:metabolism*
chemical_list: D010936:Plant Extracts; D015262:Xenobiotics; D005054:Eugenol; D019363:Cytochrome P-450 CYP1A1; D014453:Glucuronosyltransferase; D005982:Glutathione Transferase