title: Protein extraction and gel-based separation methods to analyze responses to pathogens in carnation (Dianthus caryophyllus L).
abstract: We are currently using a 2-DE-based proteomics approach to study plant responses to pathogenic fungi by using the carnation (Dianthus caryophyllus L)-Fusarium oxysporum f. sp. dianthi pathosystem. It is clear that the protocols for the first stages of a standard proteomics workflow must be optimized to each biological system and objectives of the research. The optimization procedure for the extraction and separation of proteins by 1-DE and 2-DE in the indicated system is reported. This strategy can be extrapolated to other plant-pathogen interaction systems in order to perform an evaluation of the changes in the host protein profile caused by the pathogen and to identify proteins which, at early stages, are involved or implicated in the plant defense response.
authors.affiliation: Laboratory Research in Plant Metabolic Activities, Department of Chemistry, National University of Colombia, Bogotá, Colombia.
authors.forename: Harold Duban
authors.initials: HD
authors.lastname: Ardila
authors.forename: Raquel González
authors.initials: RG
authors.lastname: Fernández
authors.forename: Blanca Ligia
authors.initials: BL
authors.lastname: Higuera
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Redondo
authors.forename: Sixta Tulia
authors.initials: ST
authors.lastname: Martínez
mesh_terms: D029749:Dianthus / Q000378:metabolism* / Q000382:microbiology*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005670:Fusarium / Q000502:physiology*; D054884:Host-Pathogen Interactions; D010940:Plant Proteins / Q000302:isolation & purification*; D013194:Staining and Labeling
chemical_list: D010940:Plant Proteins