title: Peripheral blood stem cell yield calculated using preapheresis absolute CD34+ cell count, peripheral blood volume processed, and donor body weight accurately predicts actual yield at multiple centers.
abstract: BACKGROUND
  Accurate prediction of stem cell yield is important for planning leukapheresis procedures. A formula has been published (Pierelli et al., Vox Sang 2006;91:126-34) to estimate the CD34+ dose collected on the first day of leukapheresis that was based on the preapheresis peripheral blood (PB) CD34+ counts, the blood volume processed, and the donor's weight. The aim of this study was to assess the predictive value of this formula.
  
  
  METHODS
  Data were retrospectively collected on 1126 consecutive PB stem cell harvests conducted at five institutions. Information on age, sex, diagnosis, weight, preapheresis absolute peripheral CD34+ count, total blood volume processed, and CD34+ cells harvested per kilogram of body weight on the first day of apheresis was collected.
  
  
  RESULTS
  Among donors at least 18 years old, Pearson's correlation coefficient (r) between actual yield (AY) and predicted yield (PY) was 0.76. To characterize this correlation, AY and PY were classified as being within the conventionally acceptable CD34+ doses (>2 × 10(6) -5 × 10(6) cells/kg), below this range (≤2 × 10(6) cells/kg), or above it (>5 × 10(6) cells/kg). The positive predictive value (PPV) of PY was estimated considering the distribution of AY as the "gold standard." PPV was relatively high for PY of more than 5 × 10(6) cells/kg (85%), moderate for PY of not more than 2 × 10(6) cells/kg (72%), and low for PY more than 2 × 10(6) to 5 × 10(6) cells/kg (56%). A consistent pattern was observed within institutions.
  
  
  CONCLUSIONS
  The formula of Pierelli et al. is associated with a PPV that is high, moderate, and relatively low for the corresponding predicted CD34+ doses.
authors.affiliation: Department of Stem Cell Transplantation and Cellular Therapy, The University of Texas MD Anderson Cancer Center, Houston, Texas.
authors.forename: Chitra
authors.initials: C
authors.lastname: Hosing
authors.forename: Rima M
authors.initials: RM
authors.lastname: Saliba
authors.forename: Nelson
authors.initials: N
authors.lastname: Hamerschlak
authors.forename: Jose Mauro
authors.initials: JM
authors.lastname: Kutner
authors.forename: Araci Massami
authors.initials: AM
authors.lastname: Sakashita
authors.forename: Andrea Tiemi
authors.initials: AT
authors.lastname: Kondo
authors.forename: Morgani
authors.initials: M
authors.lastname: Rodrigues
authors.forename: Juliana Folloni
authors.initials: JF
authors.lastname: Fernande
authors.forename: Alexandre
authors.initials: A
authors.lastname: Chiattone
authors.forename: Viviane C
authors.initials: VC
authors.lastname: Chiattone
authors.forename: Jose C
authors.initials: JC
authors.lastname: Barros
authors.forename: Carlos S
authors.initials: CS
authors.lastname: Chiattone
authors.forename: Ricardo
authors.initials: R
authors.lastname: Chiattone
authors.forename: Uday
authors.initials: U
authors.lastname: Popat
authors.forename: Muzaffar
authors.initials: M
authors.lastname: Qazilbash
authors.forename: Xiao Wen
authors.initials: XW
authors.lastname: Tang
authors.forename: Depei
authors.initials: D
authors.lastname: Wu
authors.forename: Alejandro
authors.initials: A
authors.lastname: Majilis
authors.forename: Marcos
authors.initials: M
authors.lastname: de Lima
authors.forename: Timoleon
authors.initials: T
authors.lastname: Anguita
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018952:Antigens, CD34 / Q000378:metabolism; D001772:Blood Cell Count / Q000379:methods; D001782:Blood Donors; D001810:Blood Volume / Q000502:physiology*; D001835:Body Weight / Q000502:physiology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007937:Leukapheresis / Q000379:methods; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation / Q000379:methods*; D011379:Prognosis; D012189:Retrospective Studies; D055815:Young Adult
chemical_list: D018952:Antigens, CD34