title: Comparative proteomics of dehydration response in the rice nucleus: new insights into the molecular basis of genotype-specific adaptation.
abstract: Dehydration is the most crucial environmental factor that considerably reduces the crop harvest index, and thus has become a concern for global agriculture. To better understand the role of nuclear proteins in water-deficit condition, a nuclear proteome was developed from a dehydration-sensitive rice cultivar IR-64 followed by its comparison with that of a dehydration-tolerant c.v. Rasi. The 2DE protein profiling of c.v. IR-64 coupled with MS/MS analysis led to the identification of 93 dehydration-responsive proteins (DRPs). Among those identified proteins, 78 were predicted to be destined to the nucleus, accounting for more than 80% of the dataset. While the detected number of protein spots in c.v. IR-64 was higher when compared with that of Rasi, the number of DRPs was found to be less. Fifty-seven percent of the DRPs were found to be common to both sensitive and tolerant cultivars, indicating significant differences between the two nuclear proteomes. Further, we constructed a functional association network of the DRPs of c.v. IR-64, which suggests that a significant number of the proteins are capable of interacting with each other. The combination of nuclear proteome and interactome analyses would elucidate stress-responsive signaling and the molecular basis of dehydration tolerance in plants.
authors.affiliation: National Institute of Plant Genome Research, New Delhi, India.
authors.forename: Dinesh Kumar
authors.initials: DK
authors.lastname: Jaiswal
authors.forename: Doel
authors.initials: D
authors.lastname: Ray
authors.forename: Mani Kant
authors.initials: MK
authors.lastname: Choudhary
authors.forename: Pratigya
authors.initials: P
authors.lastname: Subba
authors.forename: Amit
authors.initials: A
authors.lastname: Kumar
authors.forename: Jitendra
authors.initials: J
authors.lastname: Verma
authors.forename: Rajiv
authors.initials: R
authors.lastname: Kumar
authors.forename: Asis
authors.initials: A
authors.lastname: Datta
authors.forename: Subhra
authors.initials: S
authors.lastname: Chakraborty
authors.forename: Niranjan
authors.initials: N
authors.lastname: Chakraborty
mesh_terms: D000222:Adaptation, Physiological; D002467:Cell Nucleus / Q000502:physiology*; D053144:Cell Nucleus Shape; D003681:Dehydration; D005838:Genotype; D053858:Metabolic Networks and Pathways; D058977:Molecular Sequence Annotation; D009687:Nuclear Proteins / Q000378:metabolism; D012275:Oryza / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D040901:Proteomics
chemical_list: D009687:Nuclear Proteins; D010940:Plant Proteins
keywords: Comparative proteomics; Dehydration response; Genotype-specific adaptation; Interactome; Nuclear fraction; Plant proteomics