title: Tpo1-mediated spermine and spermidine export controls cell cycle delay and times antioxidant protein expression during the oxidative stress response.
abstract: Cells counteract oxidative stress by altering metabolism, cell cycle and gene expression. However, the mechanisms that coordinate these adaptations are only marginally understood. Here we provide evidence that timing of these responses in yeast requires export of the polyamines spermidine and spermine. We show that during hydrogen peroxide (H2O2) exposure, the polyamine transporter Tpo1 controls spermidine and spermine concentrations and mediates induction of antioxidant proteins, including Hsp70, Hsp90, Hsp104 and Sod1. Moreover, Tpo1 determines a cell cycle delay during adaptation to increased oxidant levels, and affects H2O2 tolerance. Thus, central components of the stress response are timed through Tpo1-controlled polyamine export.
authors.affiliation: 1] Cambridge Systems Biology Centre and Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, CB2 1GA Cambridge, UK [2] Max Planck Institute for Molecular Genetics, Ihnestr. 73, 14195 Berlin, Germany.
authors.forename: Antje
authors.initials: A
authors.lastname: Krüger
authors.forename: Jakob
authors.initials: J
authors.lastname: Vowinckel
authors.forename: Michael
authors.initials: M
authors.lastname: Mülleder
authors.forename: Phillip
authors.initials: P
authors.lastname: Grote
authors.forename: Floriana
authors.initials: F
authors.lastname: Capuano
authors.forename: Katharina
authors.initials: K
authors.lastname: Bluemlein
authors.forename: Markus
authors.initials: M
authors.lastname: Ralser
mesh_terms: D017920:Antiporters / Q000235:genetics / Q000378:metabolism*; D059447:Cell Cycle Checkpoints; D015966:Gene Expression Regulation, Fungal; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000633:toxicity; D027701:Organic Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013096:Spermine / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D013997:Time Factors
chemical_list: D017920:Antiporters; D006360:Heat-Shock Proteins; D027701:Organic Cation Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; C475058:TPO1 protein, S cerevisiae; D013096:Spermine; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1