title: Construction of adipose scaffold for bone repair with gene engineering bone cells.
abstract: The bone defect repairing is still a challenge in orthopedics. As the gene engineering bones have been used in the bone repairing clinic, the scaffold construction is a critical fact to be considered. This study aims to construct optimal scaffolds using adipose tissue in the bone repair together with the gene engineering osteocytes. Rat adipose stem cells (ASC) were prepared; the cells were transduced with the OCT-4 gene carrying lentiviral vectors (OCT-4-Lv). Artificial bone defects were created in the rat femoral bone. The bone defects were filled up with adipose scaffolds and shaped by using surrounding muscles and supported with orthopedic splints. ASCs with or without transducing the OCT-4-Lv were injected into the adipose scaffolds. The rats were sacrificed 12 weeks after the surgery. After receiving the OCT-4-Lv, the expressions of OCT-4, RUNX2 and osteocalcin were detected in the ASCs. X-ray examination showed that rats received the OCT-4-Lv transduced ASCs together with the adipose pad had new bone formation in the defect area; none of the control rats showed any new bone formation in situ. The results were supported by histological assessment. Using adipose scaffold and OCT-4-modified ASC transplantation can repair bone defects.
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Li
authors.forename: Jingzhang
authors.initials: J
authors.lastname: Fan
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Weiliang
authors.initials: W
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Su
authors.forename: Zhenggang
authors.initials: Z
authors.lastname: Bi
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D015153:Blotting, Western; D001842:Bone and Bones / Q000166:cytology / Q000293:injuries*; D005269:Femur / Q000293:injuries; D005818:Genetic Engineering / Q000379:methods*; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000502:physiology; D010011:Osteocytes / Q000737:chemistry*; D010012:Osteogenesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D050814:Octamer Transcription Factor-3
keywords: Bone defect; bone repair; engineering bone; tissue engineering; transplantation