title: Optimization of fermentation conditions and purification of cordycepin from Cordyceps militaris.
abstract: The fermentation medium and conditions for the production of cordycepin were optimized in static culture using single-factor experiments, Placket-Burman design, a central composite design, and response surface methodology. Among seven variables including temperature, pH, and the concentrations of glucose, tryptone, yeast extract, KH₂PO₄, and MgSO₄ · 7H₂O, temperature and the concentrations of yeast extract and tryptone were found to be the important factors that significantly affected cordycepin production. The optimized medium consisted of yeast extract 9.00 g/L and tryptone 17.10 g/L, while the optimized culture conditions consisted of seed age 3 days, with an inoculum size of 10% and incubation temperature of 27.1°C. A maximum cordycepin yield of 7.35 g/L was achieved in a 5-L fermenter under the optimized conditions. Next, cordycepin was partially purified and determined. The resulting product showed 90.54% high-performance liquid chromatography (HPLC)-ultraviolet (UV) purity. Therefore, cordycepin was applied to a cell viability assay on SH-SY5Y cells and RM-1 cells. Cordycepin can inhibit the proliferation of RM-1 cells with IC₅₀ of 133 µmol/L, but it has no inhibitory effect on SH-SY5Y cells. Supplemental materials are available for this article.
authors.affiliation: a Department of Biochemistry and Pharmacy , Institute of Nautical Medicine, Nantong University , Nantong , P. R. China.
authors.forename: Tang
authors.initials: T
authors.lastname: Jiapeng
authors.forename: Liu
authors.initials: L
authors.lastname: Yiting
authors.forename: Zhu
authors.initials: Z
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid; D032661:Cordyceps / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D003839:Deoxyadenosines / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D003470:Culture Media; D003839:Deoxyadenosines; C058120:cordycepin