title: Thiols and selenols as electron-relay catalysts for disulfide-bond reduction.
abstract: Pass them on! Dithiobutylamine immobilized on a resin is a useful reagent for the reduction of disulfide bonds. Its ability to reduce a disulfide bond in a protein is enhanced greatly if used along with a soluble strained cyclic disulfide or mixed diselenide that relays electrons from the resin to the protein. This electron-relay catalysis system provides distinct advantages over the use of excess soluble reducing agent alone.
authors.affiliation: Department of Chemistry, 1101 University Avenue, University of Wisconsin-Madison, Madison, WI 53706 (USA) http://www.biochem.wisc.edu/faculty/raines/lab.
authors.forename: John C
authors.initials: JC
authors.lastname: Lukesh
authors.forename: Brett
authors.initials: B
authors.lastname: Vanveller
authors.forename: Ronald T
authors.initials: RT
authors.lastname: Raines
mesh_terms: D002384:Catalysis; D004220:Disulfides / Q000737:chemistry*; D004583:Electrons; D010206:Papain / Q000737:chemistry*; D018036:Selenium Compounds / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D004220:Disulfides; D018036:Selenium Compounds; D013438:Sulfhydryl Compounds; C442270:selenol; D010206:Papain
keywords: heterogeneous catalysis; proteins; redox chemistry; selenium; sulfur