title: Neurogenic differentiation capacity of subacromial bursal tissue-derived stem cells.
abstract: In this study, analysis and comprehensive comparison of neurogenic differentiation capacity of human bursal tissue-derived-stem cells (hBT-SCs) was aimed with human bone marrow derived mesenchymal stem cells (hBM-MSCs). hBT-SCs was isolated from subacromial bursa tissue (n = 3) by collagen type-II digestion. The expression of stem cell markers, differentiation capacity and telomerase activity were determined for both cell lines. The expression levels of neurogenic cell markers were compared consecutively. With respect to the surface marker profile, both cells display similar pluripotency phenotypes. Both cells successfully differentiated into osteo- and adipogenic cell lines. The immune staining of mesenchymal, stem cell and neurogenic markers gave positive reaction. The gene expression level for Tubb3, Nestin, Gfap, Map2, Nf-h, and Nf-l was higher in hBT-SCs than hBM-MSCs. The high level of neurotrophic factors, like Tenascin C, NGF, BDNF, VEGF, and CNTF might indicate their regeneration and maintenance capacity in damaged neural tissue. Besides they are alternative source for human mesenchymal stem cells, hBT-SCs assess the possibility to use in clinical studies.
authors.affiliation: Department of Orthopedics, Seka Hospital, 41050, Kocaeli, Turkey.
authors.forename: Adem
authors.initials: A
authors.lastname: Aydın
authors.forename: Gökhan
authors.initials: G
authors.lastname: Duruksu
authors.forename: Gülay
authors.initials: G
authors.lastname: Erman
authors.forename: Cansu
authors.initials: C
authors.lastname: Subaşı
authors.forename: Ayça
authors.initials: A
authors.lastname: Aksoy
authors.forename: Zehra Seda
authors.initials: ZS
authors.lastname: Unal
authors.forename: Erdal
authors.initials: E
authors.lastname: Karaöz
mesh_terms: D000173:Acromioclavicular Joint / Q000166:cytology*; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology; D002061:Bursa, Synovial / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology*; D009457:Neuroglia / Q000166:cytology; D009474:Neurons / Q000166:cytology* / Q000502:physiology
chemical_list: D015415:Biomarkers; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins
keywords: bone marrow; bursal tissue; mesenchymal stem cell; neural differentiation