title: Oligonucleotide labelling using a fluorogenic "click" reaction with a hemicarboxonium salt.
abstract: Two fluorescent streptocyanine labelled oligonucleotides have been synthesized by a simple "click" reaction between a non-fluorescent hemicarboxonium salt and aminoalkyl functionalized thymidines within the oligonucleotide and their spectrophotometric properties have been studied.
authors.affiliation: Modified Nucleic Acids, Laboratoire de Synthèse et Physico-Chimie de Molécules d'Intérêt Biologique (UMR 5068) Université de Toulouse (Université Paul Sabatier), Cedex 9, 31062 Toulouse, France. escudier@chimie.ups-tlse.fr.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Maether
authors.forename: Kristie
authors.initials: K
authors.lastname: Lapin
authors.forename: Andreea
authors.initials: A
authors.lastname: Muntean
authors.forename: Corinne
authors.initials: C
authors.lastname: Payrastre
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Escudier
mesh_terms: D001483:Base Sequence; D002232:Carbocyanines / Q000138:chemical synthesis* / Q000737:chemistry; D057930:Click Chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D060005:Genotyping Techniques; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D013936:Thymidine / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; D013936:Thymidine