title: Asn3, a reliable, robust, and universal lock mass for improved accuracy in LC-MS and LC-MS/MS.
abstract: The use of internal calibrants (the so-called lock mass approach) provides much greater accuracy in mass spectrometry based proteomics. However, the polydimethylcyclosiloxane (PCM) peaks commonly used for this purpose are quite unreliable, leading to missing calibrant peaks in spectra and correspondingly lower mass measurement accuracy. Therefore, we here introduce a universally applicable and robust internal calibrant, the tripeptide Asn3. We show that Asn3 is a substantial improvement over PCM both in terms of consistent detection and resulting mass measurement accuracy. Asn3 is also very easy to adopt in the lab, as it requires only minor adjustments to the analytical setup.
authors.affiliation: Department of Medical Protein Research, VIB , B-9000 Ghent, Belgium.
authors.forename: An
authors.initials: A
authors.lastname: Staes
authors.forename: Jonathan
authors.initials: J
authors.lastname: Vandenbussche
authors.forename: Hans
authors.initials: H
authors.lastname: Demol
authors.forename: Marc
authors.initials: M
authors.lastname: Goethals
authors.forename: Şule
authors.initials: Ş
authors.lastname: Yilmaz
authors.forename: Niels
authors.initials: N
authors.lastname: Hulstaert
authors.forename: Sven
authors.initials: S
authors.lastname: Degroeve
authors.forename: Pieter
authors.initials: P
authors.lastname: Kelchtermans
authors.forename: Lennart
authors.initials: L
authors.lastname: Martens
authors.forename: Kris
authors.initials: K
authors.lastname: Gevaert
mesh_terms: D001216:Asparagine / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D019169:Jurkat Cells; D010446:Peptide Fragments / Q000737:chemistry*; D040901:Proteomics; D012833:Siloxanes / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010446:Peptide Fragments; D012833:Siloxanes; D001216:Asparagine