title: Early Staphylococcus aureus-induced changes in endothelial barrier function are strain-specific and unrelated to bacterial translocation.
abstract: The vascular endothelium provides the critical barrier during hematogenous spreading of bacteria, a phenomenon that might contribute to severe diseases in humans including endocarditis and sepsis as known from infections by Staphylococcus aureus. Here we aimed to uncover early responses of the endothelium to S. aureus infection with respect to (a) inflammatory reactions such as paracellular endothelial barrier function and expression of cell adhesion molecule-1 (ICAM-1) and (b) translocation through the endothelium. After infection of the cultured endothelium with 22 different clinical isolates of S. aureus and two well-characterized lab strains a diverse and strain-specific change in para- and transcellular endothelial barrier function was observed. Bayesian data analysis revealed positive correlation of paracellular barrier function decrease followed by expression of ICAM-1 while these parameters negatively correlated with transcellular bacterial translocation. Translocating bacteria largely blocked TNFα-induced ICAM-1 expression indicating an active anti-inflammatory effect mediated by those strains probably due to intracellularly released virulence factors. Furthermore, the underlying background of barrier function decrease was investigated in more detail using two well-characterized lab strains, ls 8325-4 and ls 6850 and respective mutants. Barrier function decrease was found to be independent of early cell death and early release of virulence factors into the medium, but require internalization of live bacteria. The data show for the first time that endothelial cells respond diversely to infection with different strains of S. aureus and that translocating strains downregulate inflammatory response of the endothelium. Furthermore, data indicate that S. aureus-mediated activation of the endothelium reduces bacterial translocation.
authors.affiliation: Institute of Anatomy and Vascular Biology, Westfaelische-Wilhelms University, Vesaliusweg 2-4, 48149 Muenster, Germany.
authors.forename: Nadzeya
authors.initials: N
authors.lastname: Kramko
authors.forename: Dzmitry
authors.initials: D
authors.lastname: Sinitski
authors.forename: Jochen
authors.initials: J
authors.lastname: Seebach
authors.forename: Bettina
authors.initials: B
authors.lastname: Löffler
authors.forename: Peter
authors.initials: P
authors.lastname: Dieterich
authors.forename: Christine
authors.initials: C
authors.lastname: Heilmann
authors.forename: Georg
authors.initials: G
authors.lastname: Peters
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Schnittler
mesh_terms: D018988:Bacterial Translocation; D002199:Capillary Permeability; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000382:microbiology* / Q000502:physiology*; D015870:Gene Expression; D054884:Host-Pathogen Interactions; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D013211:Staphylococcus aureus / Q000502:physiology*
chemical_list: D018799:Intercellular Adhesion Molecule-1
keywords: Bacterial translocation; Early interactions; Endothelial permeability; S. aureus