title: Comparison of graft healing in anterior cruciate ligament reconstruction with and without a preserved remnant in rabbits.
abstract: BACKGROUND
  The remnant of the native anterior cruciate ligament (ACL) might contribute to the biological integration of the graft in ACL reconstruction. The aim of this study was to explore whether the preserved remnant enhanced graft healing in ACL reconstruction.
  
  
  METHODS
  Forty New Zealand rabbits underwent bilateral anterior cruciate ligament reconstructions. One knee was treated with a 2-mm remnant preserved on the tibial side (remnant-preservation, RP group) while the contralateral knee underwent a complete removal of the remnants by cauterization (remnant-resection, RR group) in each animal. Gross observations combined with microangiography, histological evaluation, and uniaxial load testing were performed after 4, 8, and 12weeks.
  
  
  RESULTS
  The vascular density on the graft surface was statistically higher in the RP group as compared to that of the RR group at 4 (P=0.002) and 8weeks (P=0.020). Additionally, the accelerated intra-articular and intra-tunnel graft integration were histologically observed in the RP group. Histological scores in the RP group were statistically higher than the RR group at 4weeks (P=0.028 for the intra-articular healing and P=0.046 for the intra-tunnel healing) and 8weeks (P=0.031 for the intra-articular healing and P=0.014 for the intra-tunnel healing). The ultimate failure load (P=0.017), yield load (P=0.025), and stiffness (P=0.004) were statistically higher in the RP group as compared to those of the RR group, with corresponding significant differences in the failure mode (P=0.020) between the two groups at 8weeks.
  
  
  CONCLUSIONS
  The preserved remnant enhanced ACL graft healing with improved biomechanical properties in the rabbit model.
  
  
  METHODS
  Level II.
authors.affiliation: Orthopaedic Center of PLA, The 88th Hospital of PLA, Tai'an City, Shandong Province, China. Electronic address: sl88h@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.forename: Min
authors.initials: M
authors.lastname: Tian
authors.forename: Baichuan
authors.initials: B
authors.lastname: Liu
authors.forename: Yongzhong
authors.initials: Y
authors.lastname: Luo
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000601:surgery*; D000070598:Anterior Cruciate Ligament Injuries; D059549:Anterior Cruciate Ligament Reconstruction / Q000379:methods*; D001696:Biomechanical Phenomena; D004195:Disease Models, Animal; D005260:Female; D006084:Graft Rejection; D006085:Graft Survival; D011817:Rabbits; D011897:Random Allocation; D018709:Statistics, Nonparametric; D013710:Tendons / Q000601:surgery / Q000637:transplantation*; D013718:Tensile Strength / Q000502:physiology*; D020858:Tissue and Organ Harvesting / Q000379:methods; D014945:Wound Healing / Q000502:physiology*
keywords: Anterior cruciate ligament reconstruction; Biology; Graft healing; Remnant; Tendon