title: Identification of host and viral factors involved in a dissimilar resolution of a hepatitis C virus infection.
abstract: OBJECTIVE
  Hepatitis C virus (HCV) transmission from a chronic patient to a susceptible individual is a good opportunity to study viral and host factors that may influence the natural course of hepatitis C infection towards either spontaneous recovery or chronicity. To compare a documented case of a bottleneck event in the sexual transmission of HCV from a chronically infected patient to a recipient host that cleared infection.
  
  
  METHODS
  Host genetic components such as Class I and II HLA and IL28B polymorphism (rs12979860 SNPs) were identified by direct sequencing and LightMix analysis, respectively. Deep nucleotide sequence analysis of quasispecies complexity was performed using massive pyrosequencing platform (454 GS-FLX), and the CD4 specific immune response was characterized by ELISPOT.
  
  
  CONCLUSIONS
  Sequencing analysis and CD4 response highlighted several NS3-helicase domains in which an interplay between amino acid variability and CD4 immune response might have contributed either to chronicity in the donor patient or to viral clearance in the receptor (newly infected) patient.
authors.affiliation: Liver Unit. Internal Medicine. Lab. Malalties Hepàtiques, Vall d'Hebron Institut Recerca-Hospital Universitari Vall d'Hebron (VHIR-HUVH), Barcelona, Spain; Roche Diagnostics SL, Sant Cugat del Vallès, Barcelona, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Cubero
authors.forename: Josep
authors.initials: J
authors.lastname: Gregori
authors.forename: Juan I
authors.initials: JI
authors.lastname: Esteban
authors.forename: Damir
authors.initials: D
authors.lastname: García-Cehic
authors.forename: Marta
authors.initials: M
authors.lastname: Bes
authors.forename: Celia
authors.initials: C
authors.lastname: Perales
authors.forename: Esteban
authors.initials: E
authors.lastname: Domingo
authors.forename: Francisco
authors.initials: F
authors.lastname: Rodríguez-Frías
authors.forename: Silvia
authors.initials: S
authors.lastname: Sauleda
authors.forename: Rosario
authors.initials: R
authors.lastname: Casillas
authors.forename: Alex
authors.initials: A
authors.lastname: Sanchez
authors.forename: Israel
authors.initials: I
authors.lastname: Ortega
authors.forename: Rafael
authors.initials: R
authors.lastname: Esteban
authors.forename: Jaume
authors.initials: J
authors.lastname: Guardia
authors.forename: Josep
authors.initials: J
authors.lastname: Quer
mesh_terms: D000328:Adult; D000998:Antiviral Agents / Q000627:therapeutic use; D005260:Female; D005838:Genotype; D016174:Hepacivirus / Q000187:drug effects / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D019698:Hepatitis C, Chronic / Q000175:diagnosis / Q000188:drug therapy / Q000276:immunology / Q000635:transmission* / Q000821:virology; D054884:Host-Pathogen Interactions; D006801:Humans; D008297:Male; D010641:Phenotype; D012074:Remission Induction; D012747:Sexual Partners; D015229:Sexually Transmitted Diseases, Viral / Q000175:diagnosis / Q000188:drug therapy / Q000276:immunology / Q000635:transmission* / Q000821:virology; D015819:Substance Abuse, Intravenous / Q000150:complications*; D013997:Time Factors; D016896:Treatment Outcome; D017361:Viral Nonstructural Proteins / Q000235:genetics; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D000998:Antiviral Agents; D017361:Viral Nonstructural Proteins; C084422:NS3 protein, hepatitis C virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
keywords: HCV; NS3; UDPS; chronicity; quasispecies; resolution