title: The influence of Sildenafil citrate on uterine tissue perfusion and the cardiovascular system during the luteal phase of the ovarian cycle in cows.
abstract: The aim of the study was to evaluate the influence of the Sildenafil citrate on the blood flow in the uterus of cows during dioestrus. Uterine blood flow was examined in five, healthy, adult cows. Between day 6-8 of the ovarian cycle, each cow received 200mg of sildenafil diluted in 10ml of warm saline into the body of the uterus. Analysis of the blood pressure, ECG and the maximum velocity in m/s (V max) in the aorta was performed and selected parameters of the blood flow (PI, pulsatile index; RI, resistance index; SPV, systolic peak velocity; EDV, end diastolic velocity; FVI, flow velocity integral; SV/DV, systolic peak velocity: end-diastolic velocity ratio) were measured in the uterine artery (Arteria uterine) before and after sildenafil infusion. In addition, Color Doppler examination of the uterine wall perfusion was analyzed. A significant decrease of values of PI and SV/DV ratio as well as an increase of end diastolic velocity and time averaged maximum velocity was noted. With the use of color coded sonography, the increased intensity of the blood flow in the uterine wall was observed. It was concluded that intrauterine administration of sildenafil during dioestrus can increase uterine tissue perfusion.
authors.affiliation: Department of Reproduction and Clinic of Farm Animals, Wrocław University of Environmental and Life Sciences, Plac Grunwaldzki 49, 50-366, Wrocław, Poland. Electronic address: michaldzieciol@wp.pl.
authors.forename: Michał
authors.initials: M
authors.lastname: Dzięcioł
authors.affiliation: Department of Reproduction and Clinic of Farm Animals, Wrocław University of Environmental and Life Sciences, Plac Grunwaldzki 49, 50-366, Wrocław, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Stańczyk
authors.affiliation: Department of Internal and Parasitic Diseases and Clinic for Horses, Dogs and Cats, Wrocław University of Environmental and Life Sciences, 47 Grunwaldzki Sq, 50-366, Wrocław, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Noszczyk-Nowak
authors.affiliation: Department of Reproduction and Clinic of Farm Animals, Wrocław University of Environmental and Life Sciences, Plac Grunwaldzki 49, 50-366, Wrocław, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Michlik
authors.affiliation: Department of Reproduction and Clinic of Farm Animals, Wrocław University of Environmental and Life Sciences, Plac Grunwaldzki 49, 50-366, Wrocław, Poland.
authors.forename: Roland
authors.initials: R
authors.lastname: Kozdrowski
authors.affiliation: Department of Reproduction and Clinic of Farm Animals, Wrocław University of Environmental and Life Sciences, Plac Grunwaldzki 49, 50-366, Wrocław, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Niżański
authors.affiliation: Department of Reproduction and Clinic of Farm Animals, Wrocław University of Environmental and Life Sciences, Plac Grunwaldzki 49, 50-366, Wrocław, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Pasławskab
authors.affiliation: Department of Reproduction and Clinic of Farm Animals, Wrocław University of Environmental and Life Sciences, Plac Grunwaldzki 49, 50-366, Wrocław, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Mrowiec
authors.affiliation: Department of Reproduction and Clinic of Farm Animals, Wrocław University of Environmental and Life Sciences, Plac Grunwaldzki 49, 50-366, Wrocław, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Twardoń
mesh_terms: D000818:Animals; D002319:Cardiovascular System / Q000187:drug effects*; D002417:Cattle; D005260:Female; D008183:Luteal Phase / Q000187:drug effects*; D008597:Menstrual Cycle; D010879:Piperazines / Q000494:pharmacology*; D011687:Purines / Q000494:pharmacology; D000068677:Sildenafil Citrate; D013450:Sulfones / Q000494:pharmacology*; D014599:Uterus / Q000098:blood supply / Q000187:drug effects*
chemical_list: D010879:Piperazines; D011687:Purines; D013450:Sulfones; D000068677:Sildenafil Citrate
keywords: Blood flow; Color Doppler; Cow; Sildenafil citrate; Tissue perfusion; Uterus