title: pIMP-PH114 carrying bla IMP-4 in a Klebsiella pneumoniae strain is closely related to other multidrug-resistant IncA/C2 plasmids.
abstract: The IncA/C plasmids are broad host-range vehicles which have been associated with wide dissemination of CMY-2 among Enterobacteriaceae of human and animal origins. Acquired metallo-β-lactamases (MBLs) such as the IMP-type enzymes are increasingly reported in multidrug-resistant Gram-negative bacteria worldwide, particularly in Enterobacteriaceae. We described the complete sequence of the first IMP-4-encoding IncA/C2 plasmid, pIMP-PH114 (151,885 bp), from a sequence type 1 Klebsiella pneumoniae strain that was recovered from a patient who was hospitalized in the Philippines. pIMP-PH114 consists of a backbone from the IncA/C2 plasmids, with the insertion of a novel Tn21-like class 1 integron composite structure (containing the cassette array bla IMP-4-qacG-aacA4-catB3, followed by a class C β-lactamase bla DHA-1 and the mercury resistance operon, merRTPCADE) and a sul2-floR encoding region. Phylogenetic analysis of the IncA/C repA sequences showed that pIMP-PH114 formed a subgroup with other IncA/C plasmids involved in the international spread of CMY-2, TEM-24 and NDM-1. Identical bla IMP-4 arrays have been described among different Enterobacteriaceae and Acinetobacter spp. in China, Singapore and Australia but the genetic context is different. The broad host range of IncA/C plasmids may have facilitated dissemination of the bla IMP-4 arrays among different diverse groups of bacteria.
authors.forename: Pak-Leung
authors.initials: PL
authors.lastname: Ho
authors.forename: Wai-U
authors.initials: WU
authors.lastname: Lo
authors.forename: Jane
authors.initials: J
authors.lastname: Chan
authors.forename: Yuk-Yam
authors.initials: YY
authors.lastname: Cheung
authors.forename: Kin-Hung
authors.initials: KH
authors.lastname: Chow
authors.forename: Wing-Cheong
authors.initials: WC
authors.lastname: Yam
authors.forename: Chi-Ho
authors.initials: CH
authors.lastname: Lin
authors.forename: Tak-Lun
authors.initials: TL
authors.lastname: Que
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D023061:Gene Order; D005838:Genotype; D007711:Klebsiella pneumoniae / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; C425142:beta-lactamase IMP-4; D001618:beta-Lactamases