title: Establishment of tracking system for West Nile virus entry and evidence of microtubule involvement in particle transport.
abstract: West Nile virus (WNV) is one of flaviviruses and has emerged recently in the United States as a significant cause of viral encephalitis. Although cellular entry of WNV is important for viral pathogenesis, its mechanisms have not been elucidated fully. To explore the entry mechanisms, a virus-particle tracking system in live cells by using fluorescently labeled subviral particles (SVPs) and time-lapse epifluorescence microscopy was established. This study revealed that, following cellular entry, SVP movements could be divided into two phases: early (slow movement) and late (fast movement) phase. Moreover, fast viral particle movements at the late phase correlated with SVP-microtubule association.
authors.affiliation: Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Makino
authors.forename: Tadaki
authors.initials: T
authors.lastname: Suzuki
authors.forename: Rie
authors.initials: R
authors.lastname: Hasebe
authors.forename: Takashi
authors.initials: T
authors.lastname: Kimura
authors.forename: Akihiko
authors.initials: A
authors.lastname: Maeda
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Takahashi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Sawa
mesh_terms: D000818:Animals; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D008870:Microtubules / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods*; D014773:Virology / Q000379:methods*; D053586:Virus Internalization; D014902:West Nile virus / Q000502:physiology*
keywords: Microtubules; Viral entry; West Nile virus