title: Label-free quantitative imaging of cholesterol in intact tissues by hyperspectral stimulated Raman scattering microscopy.
abstract: A finger on the pulse: Current molecular analysis of cells and tissues routinely relies on separation, enrichment, and subsequent measurements by various assays. Now, a platform of hyperspectral stimulated Raman scattering microscopy has been developed for the fast, quantitative, and label-free imaging of biomolecules in intact tissues using spectroscopic fingerprints as the contrast mechanism.
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47906 (USA).
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Junjie
authors.initials: J
authors.lastname: Li
authors.forename: Pu
authors.initials: P
authors.lastname: Wang
authors.forename: Chun-Rui
authors.initials: CR
authors.lastname: Hu
authors.forename: Delong
authors.initials: D
authors.lastname: Zhang
authors.forename: Michael
authors.initials: M
authors.lastname: Sturek
authors.forename: Ji-Xin
authors.initials: JX
authors.lastname: Cheng
mesh_terms: D001158:Arteries / Q000737:chemistry; D050197:Atherosclerosis / Q000473:pathology; D002784:Cholesterol / Q000032:analysis / Q000737:chemistry*; D006801:Humans; D013059:Spectrum Analysis, Raman / Q000379:methods*; D046888:Tissue Array Analysis / Q000379:methods*
chemical_list: D002784:Cholesterol
keywords: cholesterol; lipids; stimulated Raman scattering; tissue analysis; vibrational spectroscopy