title: A novel laccase with inhibitory activity towards HIV-I reverse transcriptase and antiproliferative effects on tumor cells from the fermentation broth of mushroom Pleurotus cornucopiae.
abstract: A novel laccase with a molecular mass of 67 kDa was isolated from the fermentation broth of Pleurotus cornucopiae through ion exchange chromatography and gel filtration. The optimal pH and temperature for the laccase was pH 4.2 and 30°C, respectively. The laccase activity was remarkably inhibited by Fe(3+) and Hg(2+) , while it was stimulated by Cu(2+) and Pb(2+) . It inhibited proliferation of the hepatoma cells HepG2 and the breast cancer cells MCF-7, and the activity of HIV-I reverse transcriptase with IC50 values of 3.9, 7.6 and 3.7 μM, respectively.
authors.affiliation: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing, 100081, China; State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, 2 Yuanmingyuan Westroad, Beijing, 100193, China.
authors.forename: Xiangli
authors.initials: X
authors.lastname: Wu
authors.forename: Chenyang
authors.initials: C
authors.lastname: Huang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.forename: Hexiang
authors.initials: H
authors.lastname: Wang
authors.forename: Jinxia
authors.initials: J
authors.lastname: Zhang
mesh_terms: D019380:Anti-HIV Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000494:pharmacology*; D005285:Fermentation; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors*; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D020076:Pleurotus / Q000201:enzymology*; D013696:Temperature
chemical_list: D019380:Anti-HIV Agents; D017077:Culture Media, Conditioned; D005656:Fungal Proteins; D042845:Laccase; D054303:HIV Reverse Transcriptase
keywords: FPLC; Pleurotus cornucopiae; fermentation broth; isolation; laccase; mushroom