title: Characterization of a new chronic lymphocytic leukemia cell line for mechanistic in vitro and in vivo studies relevant to disease.
abstract: Studies of chronic lymphocytic leukemia (CLL) have yielded substantial progress, however a lack of immortalized cell lines representative of the primary disease has hampered a full understanding of disease pathogenesis and development of new treatments. Here we describe a novel CLL cell line (OSU-CLL) generated by EBV transformation, which displays a similar cytogenetic and immunophenotype observed in the patient's CLL (CD5 positive with trisomy 12 and 19). A companion cell line was also generated from the same patient (OSU-NB). This cell line lacked typical CLL characteristics, and is likely derived from the patient's normal B cells. In vitro migration assays demonstrated that OSU-CLL exhibits migratory properties similar to primary CLL cells whereas OSU-NB has significantly reduced ability to migrate spontaneously or towards chemokine. Microarray analysis demonstrated distinct gene expression patterns in the two cell lines, including genes on chromosomes 12 and 19, which is consistent with the cytogenetic profile in this cell line. Finally, OSU-CLL was readily transplantable into NOG mice, producing uniform engraftment by three weeks with leukemic cells detectable in the peripheral blood spleen and bone marrow. These studies describe a new CLL cell line that extends currently available models to study gene function in this disease.
authors.affiliation: Department of Internal Medicine, Division of Hematology, Comprehensive Cancer Center at The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Erin
authors.initials: E
authors.lastname: Hertlein
authors.forename: Kyle A
authors.initials: KA
authors.lastname: Beckwith
authors.forename: Gerard
authors.initials: G
authors.lastname: Lozanski
authors.forename: Timothy L
authors.initials: TL
authors.lastname: Chen
authors.forename: William H
authors.initials: WH
authors.lastname: Towns
authors.forename: Amy J
authors.initials: AJ
authors.lastname: Johnson
authors.forename: Amy
authors.initials: A
authors.lastname: Lehman
authors.forename: Amy S
authors.initials: AS
authors.lastname: Ruppert
authors.forename: Brad
authors.initials: B
authors.lastname: Bolon
authors.forename: Leslie
authors.initials: L
authors.lastname: Andritsos
authors.forename: Arletta
authors.initials: A
authors.lastname: Lozanski
authors.forename: Laura
authors.initials: L
authors.lastname: Rassenti
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Zhao
authors.forename: Tiina M
authors.initials: TM
authors.lastname: Jarvinen
authors.forename: Leigha
authors.initials: L
authors.lastname: Senter
authors.forename: Carlo M
authors.initials: CM
authors.lastname: Croce
authors.forename: David E
authors.initials: DE
authors.lastname: Symer
authors.forename: Albert
authors.initials: A
authors.lastname: de la Chapelle
authors.forename: Nyla A
authors.initials: NA
authors.lastname: Heerema
authors.forename: John C
authors.initials: JC
authors.lastname: Byrd
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002465:Cell Movement; D002472:Cell Transformation, Viral; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics / Q000473:pathology*; D051379:Mice; D010641:Phenotype