title: No detection of the NS5B S282T mutation in treatment-naïve genotype 1 HCV/HIV-1 coinfected patients using deep sequencing.
abstract: BACKGROUND
  The S282T mutation is the main variant described associated with resistance to nucleos(t)ide analogues hepatitis C virus (HCV) NS5B polymerase inhibitors.
  
  
  OBJECTIVE
  We aimed here to investigate whether this substitution pre-existed in treatment naive HCV/HIV-1 coinfected patients.
  
  
  METHODS
  NS5B polymerase deep sequencing was performed at a median coverage per base of 4471 in 16 patient samples.
  
  
  RESULTS
  No S282T variant was detected in the 16 analyzed samples.
  
  
  CONCLUSIONS
  This finding is in agreement with the high genetic barrier of nucleoside analogues NS5B polymerase inhibitors and the clinical efficacy of these compounds.
authors.affiliation: Fundació irsiCaixa, Hospital Universitari Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Spain.
authors.forename: Sandra
authors.initials: S
authors.lastname: Franco
authors.forename: Maria
authors.initials: M
authors.lastname: Casadellà
authors.forename: Marc
authors.initials: M
authors.lastname: Noguera-Julian
authors.forename: Bonaventura
authors.initials: B
authors.lastname: Clotet
authors.forename: Cristina
authors.initials: C
authors.lastname: Tural
authors.forename: Roger
authors.initials: R
authors.lastname: Paredes
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Martinez
mesh_terms: D000595:Amino Acid Sequence; D000998:Antiviral Agents / Q000494:pharmacology; D001483:Base Sequence; D015331:Cohort Studies; D060085:Coinfection; D024882:Drug Resistance, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D016174:Hepacivirus / Q000201:enzymology / Q000235:genetics*; D006526:Hepatitis C / Q000821:virology*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D012367:RNA, Viral / Q000097:blood; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D017386:Sequence Homology, Amino Acid; D017361:Viral Nonstructural Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase
chemical_list: D000998:Antiviral Agents; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase
keywords: Deep sequencing; HCV; NS5B; Resistance; S282T