title: Synergism between propolis and hyperthermal intraperitoneal chemotherapy with cisplatin on ehrlich ascites tumor in mice.
abstract: We investigated antitumor, genotoxic, chemopreventive, and immunostimulative effects of local chemoimmunotherapy and hyperthermal intraperitoneal chemotherapy (HIPEC) in a mouse-bearing Ehrlich ascites tumor (EAT). Mice were treated with water-soluble derivative of propolis (WSDP) at a dose of 50 mg kg(-1) , 7 and 3 days before implantation of EAT cells, whereas cisplatin (5 or 10 mg kg(-1) ) was injected 3 days after implantation of EAT cells at 37°C and 43°C. The following variables were analyzed: the total number of cells, differential count of the cells present in the peritoneal cavity, functional activity of macrophages, comet assay, and micronucleus assay. The combination of WSDP + CIS 5 mg kg(-1) at 37°C resulted in tumor growth inhibition and increased the survival of mice by additional 115.25%. WSDP with HIPEC increased the survival of mice by additional 160.3% as compared with HIPEC. WSDP reduced cisplatin toxic and genotoxic effect to normal cells without affecting cisplatin cytotoxicity on EAT cells. In addition, WSDP with HIPEC increased the cytotoxic actions of macrophages to tumor cells. Water-soluble derivative of propolis increases macrophage activity and sensitivity of tumor cells to HIPEC and reduces cisplatin toxicity to normal cells.
authors.affiliation: Department of Animal Physiology, Faculty of Science, University of Zagreb, Zagreb, 10000, Croatia.
authors.forename: Nada
authors.initials: N
authors.lastname: Oršolić
authors.forename: Nikola
authors.initials: N
authors.lastname: Car
authors.forename: Duje
authors.initials: D
authors.lastname: Lisičić
authors.forename: Vesna
authors.initials: V
authors.lastname: Benković
authors.forename: Anica Horvat
authors.initials: AH
authors.lastname: Knežević
authors.forename: Domagoj
authors.initials: D
authors.lastname: Dikić
authors.forename: József
authors.initials: J
authors.lastname: Petrik
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D002945:Cisplatin / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D004357:Drug Synergism; D006979:Hyperthermia, Induced; D007167:Immunotherapy; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D011429:Propolis / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000276:Adjuvants, Immunologic; D000970:Antineoplastic Agents; D011429:Propolis; D002945:Cisplatin
keywords: Ehrlich ascites tumorp; cancer chemopreveention; chemotherapy; cisplatin; ehrlich ascites tumor; food interaction; hyperthemia; hyperthermia; immunomodulation; immunotherapy; natural products; propolis; toxicity