title: Dyeing and antibacterial activation with methylene blue of a cyclodextrin modified polyester vascular graft.
abstract: The aim of this study was to develop an antiseptic and blue dyed polyester (PET) vascular graft in order to reach two distinct properties: (i) the prevention of postoperative infections, (ii) the improvement of the graft compatibility with the coelioscopy surgical technique. This work consisted of dyeing a vascular prosthesis with methylene blue (MB) which is known as a cationic dye with antiseptic properties. Therefore, the functionalization of the PET fibers of the prosthesis with a cyclodextrin-citric acid polymer (PolyCD) was achieved in order to improve its sorption capacity. The NMR experiments demonstrated that a 1:2 complex occurred between hydroxypropyl β-cyclodextrin (HP-βCD) and MB. Kinetic and sorption isotherm studies showed that an impregnation of the polyCD modified prosthesis (PET-CD) in a 1 g L(-1) of MB solution for 150 min was sufficient to reach the saturation of the device. Results proved that the adsorption mechanism followed the Langmuir model and a maximum of 20 mg g(-1) of MB on the graft. A sustained release of MB in batch tests was observed in PBS and in vitro microbiological assays displayed a prolongation of the bactericidal effect of PET-CD whose extent varied with the amount of MB preliminarily adsorbed onto the PET-CD.
authors.affiliation: University of Lille Nord de France, 59000, Lille, France; Unité Matériaux Et Transformation (UMET), Ingénierie des Systèmes Polymères, Université Lille 1, 59655, Villeneuve D'Ascq, France.
authors.forename: I
authors.initials: I
authors.lastname: Kacem
authors.forename: T
authors.initials: T
authors.lastname: Laurent
authors.forename: N
authors.initials: N
authors.lastname: Blanchemain
authors.forename: C
authors.initials: C
authors.lastname: Neut
authors.forename: F
authors.initials: F
authors.lastname: Chai
authors.forename: S
authors.initials: S
authors.lastname: Haulon
authors.forename: H F
authors.initials: HF
authors.lastname: Hildebrand
authors.forename: B
authors.initials: B
authors.lastname: Martel
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology; D001807:Blood Vessel Prosthesis / Q000382:microbiology*; D002460:Cell Line; D002482:Cellulose / Q000737:chemistry*; D003505:Cyclodextrins / Q000737:chemistry*; D006801:Humans; D008751:Methylene Blue / Q000008:administration & dosage* / Q000494:pharmacology; D011091:Polyesters / Q000737:chemistry*; D013203:Staphylococcal Infections / Q000517:prevention & control; D013212:Staphylococcus epidermidis / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D003505:Cyclodextrins; D011091:Polyesters; C041953:cyclodextrin polymer; D002482:Cellulose; D008751:Methylene Blue
keywords: adsorption isotherm; antibacterial activity; cyclodextrin; methylene blue; vascular prosthesis