title: Characterisation of honeys according to their content of phenolic compounds using high performance liquid chromatography/tandem mass spectrometry.
abstract: A simple, fast and specific high performance liquid chromatography separation with an electro-spray ionisation tandem mass spectrometry detection in a negative single reaction ion monitoring scan mode was developed and used for the characterization of Polish honeys according to the content of phenolic acids, including caffeic, chlorogenic, p-coumaric, ferulic, homogentisic, p-hydroxybenzoic and vanillic acids, and flavonoids, i.e., apigenin, genistein, hesperetin, kaempferol, luteolin, rhamnetin, rutin, tricetin and quercetin. Target compounds were isolated and pre-concentrated from the honey matrix by means of the solid phase extraction using Strata X (500mg) cartridges. Analysed honeys did not contain tricetin and genistein. Hesperetin was determined for the first time in heather and linden honeys while rutin in rape honey.
authors.affiliation: Department of Biotechnology, Faculty of Biological Sciences, University of Zielona Gora, Prof. Z. Szafrana 1, 65-516 Zielona Gora, Poland. Electronic address: i.sergiel@wnb.uz.zgora.pl.
authors.forename: Iwona
authors.initials: I
authors.lastname: Sergiel
authors.forename: Pawel
authors.initials: P
authors.lastname: Pohl
authors.forename: Magdalena
authors.initials: M
authors.lastname: Biesaga
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000737:chemistry*; D006722:Honey / Q000032:analysis*; D062385:Hydroxybenzoates / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005419:Flavonoids; D062385:Hydroxybenzoates; C017616:phenolic acid
keywords: Flavonoids; HPLC–ESI-MS/MS; Honey; Phenolic acids; SPE; SRM