title: [Significance of erythrocytes phosphatidylserine exposure on occurrence of anemia in sepsis].
abstract: OBJECTIVE
  To investigate the role of phosphatidylserine (PS) exposure of erythrocytes in the development of anemia in sepsis patients.
  
  
  METHODS
  A self-control study was conducted. Thirty sepsis patients admitted to intensive care unit (ICU) were enrolled. Peripheral venous blood was collected on 1 day and 7 days after ICU admission, and hemoglobin (Hb) concentration was examined routinely. A flow-cytometric assay based on Annexin V/ propidium iodide (Annexin V/PI) was used to measure the PS exposure of erythrocytes. The relationship between PS exposure and Hb concentration was analyzed.
  
  
  RESULTS
  Hb concentrations in 30 sepsis patients at 7 days after ICU admission were significantly decreased compared with those of patients at 1 day (81.59±3.31 g/L vs. 121.90±3.34 g/L, t=8.570, P=0.000), but the percentage of PS exposure of erythrocytes was significantly higher [(17.19±1.35)% vs. (7.87±0.83)%, t=-6.557, P=0.000]. An inverse correlation was found between percentage of PS-positive RBCs and Hb concentration by Pearson analysis (r=-0.838, P=0.000).
  
  
  CONCLUSIONS
  The percentage of PS exposure in erythrocytes is significantly increased in sepsis, and it might contribute to the development of anemia in sepsis patients during hospital stay. The more severe the anemia, the higher the PS in erythrocytes.
authors.affiliation: Department of Emergency Critical Care Medicine, Shanghai Songjiang Center Hospital, Shanghai 201600, China, Corresponding author: Shi Bin, Email: joysb1969@sina.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Shi
authors.forename: Guo-guang
authors.initials: GG
authors.lastname: Ma
authors.forename: Hong-xiang
authors.initials: HX
authors.lastname: Liu
authors.forename: Jing-quan
authors.initials: JQ
authors.lastname: Liu
authors.forename: Wan-hua
authors.initials: WH
authors.lastname: Yang
authors.forename: Hong-ze
authors.initials: HZ
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Sheng-xia
authors.initials: SX
authors.lastname: Wan
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000740:Anemia / Q000209:etiology; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010718:Phosphatidylserines / Q000097:blood*; D011379:Prognosis; D018805:Sepsis / Q000097:blood* / Q000150:complications
chemical_list: D010718:Phosphatidylserines