title: Are anti-inflammatory properties of lipoic acid associated with the formation of hydrogen sulfide?
abstract: BACKGROUND
  Lipoic acid (LA) was shown to possess anti-inflammatory properties. In this study, we present evidence supporting the hypothesis that the anti-inflammatory properties of LA are associated with the formation of hydrogen sulfide (H2S).
  
  
  METHODS
  The study was conducted on male albino Swiss mice. The animals were treated with carrageenan by subcutaneous (sc) injection into the right hind paw to induce acute inflammation. Animals were treated intraperitoneally (ip) with LA (30, 50 and 100 mg/kg) or indomethacin (20 mg/kg) 30 min before carrageenan administration. The control group was given ip the vehicle (1% Tween 80) 30 min before carrageenan administration. Additional experiment involved ip combined treatment of mice with glibenclamide (10 mg/kg) or glibenclamide (10 mg/kg) and LA(100 mg/kg) 30 min before carrageenan administration. LA, indomethacin and glibenclamide were suspended in 1% Tween 80. At 1, 2 and 3 h after treatment with carrageenan the degree of the paw edema was evaluated by the measurement of the paw volume using aqueous plethysmometer.
  
  
  RESULTS
  Injection of carrageenan into the mouse hind paw increased paw volume. The increase in paw edema was completely suppressed by pretreatment with LA. The reduction of paw edema by LA was abolished by pretreatment with the K(ATP) channel antagonist, glibenclamide.
  
  
  CONCLUSIONS
  Our findings demonstrate for the first time in vivo that the anti-inflammatory activity of LA might be connected with the formation of H2S.
authors.affiliation: Department of Pharmacodynamics, Jagiellonian University, Collegium Medicum, Medyczna 9, PL 30-688 Kraków, Poland. magda.dudek@uj.edu.pl.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Dudek
authors.forename: Anna
authors.initials: A
authors.lastname: Bilska-Wilkosz
authors.forename: Joanna
authors.initials: J
authors.lastname: Knutelska
authors.forename: Szczepan
authors.initials: S
authors.lastname: Mogilski
authors.forename: Marek
authors.initials: M
authors.lastname: Bednarski
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Zygmunt
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Iciek
authors.forename: Jacek
authors.initials: J
authors.lastname: Sapa
authors.forename: Dominik
authors.initials: D
authors.lastname: Bugajski
authors.forename: Barbara
authors.initials: B
authors.lastname: Filipek
authors.forename: Lidia
authors.initials: L
authors.lastname: Włodek
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002351:Carrageenan; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D005905:Glyburide / Q000494:pharmacology; D006614:Hindlimb / Q000187:drug effects; D006862:Hydrogen Sulfide / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology / Q000627:therapeutic use; D008297:Male; D051379:Mice; D026902:Potassium Channel Blockers / Q000494:pharmacology; D008063:Thioctic Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000893:Anti-Inflammatory Agents; D026902:Potassium Channel Blockers; D008063:Thioctic Acid; D002351:Carrageenan; D005905:Glyburide; D007213:Indomethacin; D006862:Hydrogen Sulfide