title: Davallialactone reduces inflammation and repairs dentinogenesis on glucose oxidase-induced stress in dental pulp cells.
abstract: BACKGROUND
  The chronic nature of diabetes mellitus (DM) raises the risk of oral complication diseases. In general, DM causes oxidative stress to organs. This study aimed to evaluate the cellular change of dental pulp cells against glucose oxidative stress by glucose oxidase with a high glucose state. The purpose of this study was to test the antioxidant character of davallialactone and to reduce the pathogenesis of dental pulp cells against glucose oxidative stress.
  
  
  METHODS
  The glucose oxidase with a high glucose concentration was tested for hydroxy peroxide (H2O2) production, cellular toxicity, reactive oxygen species (ROS) formation, induction of inflammatory molecules and disturbance of dentin mineralization in human dental pulp cells. The anti-oxidant effect of Davallilactone was investigated to restore dental pulp cells' vitality and dentin mineralization via reduction of H2O2 production, cellular toxicity, ROS formation and inflammatory molecules.
  
  
  RESULTS
  The treatment of glucose oxidase with a high glucose concentration increased H2O2 production, cellular toxicity, and inflammatory molecules and disturbed dentin mineralization by reducing pulp cell activity. However, davallialactone reduced H2O2 production, cellular toxicity, ROS formation, inflammatory molecules, and dentin mineralization disturbances even with a long-term glucose oxidative stress state.
  
  
  CONCLUSIONS
  The results of this study imply that the development of oral complications is related to the irreversible damage of dental pulp cells by DM-induced oxidative stress. Davallialactone, a natural antioxidant, may be useful to treat complicated oral disease, representing an improvement for pulp vital therapy.
authors.affiliation: Department of Oral Biochemistry and Institute of Oral Bioscience, BK21 Program, Jeonju, Korea; Division of Biotechnology, College of Environmental and Biosource Science, Chonbuk National University, Jeonju, Korea.
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Lee
authors.forename: Go-Eun
authors.initials: GE
authors.lastname: Kim
authors.forename: Yong-Beom
authors.initials: YB
authors.lastname: Song
authors.forename: Usha
authors.initials: U
authors.lastname: Paudel
authors.forename: Nan-Hee
authors.initials: NH
authors.lastname: Lee
authors.forename: Bong-Sik
authors.initials: BS
authors.lastname: Yun
authors.forename: Mi-Kyung
authors.initials: MK
authors.lastname: Yu
authors.forename: Ho-Keun
authors.initials: HK
authors.lastname: Yi
mesh_terms: D000363:Agaricales; D000469:Alkaline Phosphatase / Q000032:analysis; D042501:Angiogenic Proteins / Q000032:analysis; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000187:drug effects; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D003804:Dentin / Q000187:drug effects; D003810:Dentinogenesis / Q000187:drug effects*; D003920:Diabetes Mellitus / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005949:Glucose Oxidase / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000378:metabolism; D018836:Inflammation Mediators / Q000032:analysis; D007783:Lactones / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology; D011671:Pulpitis / Q000517:prevention & control*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014074:Tooth Calcification / Q000187:drug effects
chemical_list: D042501:Angiogenic Proteins; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016207:Cytokines; D018836:Inflammation Mediators; D007783:Lactones; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C068766:davallialactone; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D000469:Alkaline Phosphatase; D005947:Glucose
keywords: Davallialactone; dental pulp cells; dentin mineralization; diabetes mellitus; glucose oxidase; glucose oxidative stress; inflammation