title: Plant cell lines in cell morphogenesis research.
abstract: Plant organs and tissues consist of many various cell types, often in different phases of their development. Such complex structures do not allow direct studies on behavior of individual cells. In contrast, populations of in vitro-cultured plant cells represent valuable tool for studying processes on a single-cell level, including cell morphogenesis. Here we describe characteristics of well-established model tobacco and Arabidopsis cell lines and provide detailed protocol on their cultivation, characterization, and genetic transformation.
authors.affiliation: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Daniela
authors.initials: D
authors.lastname: Seifertová
authors.forename: Petr
authors.initials: P
authors.lastname: Klíma
authors.forename: Markéta
authors.initials: M
authors.lastname: Pařezová
authors.forename: Jan
authors.initials: J
authors.lastname: Petrášek
authors.forename: Eva
authors.initials: E
authors.lastname: Zažímalová
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Opatrný
mesh_terms: D017360:Arabidopsis / Q000033:anatomy & histology* / Q000254:growth & development*; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D008938:Mitosis; D059828:Plant Cells / Q000502:physiology*; D063245:Plant Development; D012106:Research