title: Intravenous immunoglobulin modulates the expansion and cytotoxicity of CD8+ T cells.
abstract: Intravenous immunoglobulin (IVIg) is successfully used in the treatment of autoimmune diseases involving self-reactive CD8(+) T cells. However, its direct influence on the cytotoxic response remains unknown. Using an antigen cross-presentation assay and a mouse model of ovalbumin (OVA) immunization, we showed that IVIg decreases the in vitro activation, proliferation and cytokine secretion of OVA-specific CD8(+) T cells (OT-I), as well as the in vivo generation of OVA-specific CD8(+) T cells. In addition, IVIg significantly decreases the proportion of perforin- and CD107a-expressing CD8(+) T cells, and inhibits the cytotoxic activity of OVA-activated OT-I cells. The interference of IVIg with the CD8(+) T-cell response is associated with T-cell receptor blockade, therefore reducing the interaction between effector and target cells. A similar blockade is observed on human CD8(+) T cells, suggesting that the observations reported here could apply to the IVIg-mediated improvement of CD8(+) T-cell-mediated autoimmune conditions in human patients.
authors.affiliation: Department of Research and Development, Héma-Québec, Québec, QC, Canada; Department of Biochemistry, Microbiology and Bioinformatics, Laval University, Québec, QC, Canada.
authors.forename: Patrick
authors.initials: P
authors.lastname: Trépanier
authors.forename: Dominique
authors.initials: D
authors.lastname: Chabot
authors.forename: Renée
authors.initials: R
authors.lastname: Bazin
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D005260:Female; D007114:Immunization; D016756:Immunoglobulins, Intravenous / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology
chemical_list: D016756:Immunoglobulins, Intravenous; D010047:Ovalbumin
keywords: CD8+ T cells; cross-presentation; cytotoxicity; immunization; intravenous immunoglobulin