title: Water-enabled visual detection of DNA.
abstract: A water-enabled visual detection strategy has been developed for the sequence-specific identification of target DNA. The conceptual basis of the assay scheme, water condensation, is environmentally friendly and chemical transformation-free, thus offering significant assay advantages over conventional diagnostic systems. This label-free strategy operates on a target-driven generation of a hydrophilic structure and alteration of surface wettability and, consequently, transition of morphological state of and light propagation mode in the surface-condensed water. The chip array detection system, implemented herein with the ligase chain reaction-rolling circle amplification protocol, has allowed the achievement of high sensitivity (600 copies), high selectivity (single-base discrimination specificity), and multiplexed analysis capability.
authors.affiliation: Department of Polymer Science and Engineering, School of Chemistry and Chemical Engineering, State Key Laboratory of Coordination Chemistry, Nanjing National Laboratory of Microstructures, Nanjing University , Nanjing 210093, China.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Liu
authors.forename: Huaxin
authors.initials: H
authors.lastname: Yao
authors.forename: Jin
authors.initials: J
authors.lastname: Zhu
mesh_terms: D004247:DNA / Q000032:analysis*; D020411:Oligonucleotide Array Sequence Analysis; D014867:Water / Q000737:chemistry*
chemical_list: D014867:Water; D004247:DNA