title: Activation of PPAR α and PPAR β/δ regulates Sertoli cell metabolism.
abstract: The purpose of this study was to evaluate the existence of a possible simultaneous regulation of fatty acid (FA) metabolism and lactate production by PPAR α and PPAR β/δ activation in Sertoli cells (SC). SC cultures obtained from 20-day-old rats were incubated with WY14643 or GW0742-pharmacological activators of PPAR α and PPAR β/δ respectively. The fatty acid transporter CD36, carnitine palmitoyltransferase 1, long- and medium-chain 3-hydroxyacyl-CoA dehydrogenases mRNA levels were analyzed. An increase in the above-mentioned genes in response to activation of both nuclear receptors was observed. Additionally, PPAR β/δ activation increased lactate production as a consequence of increased pyruvate availability by inhibiting the Pyruvate Dehydrogenase Complex. Altogether, these results suggest that in SC, PPAR α activation participates in the regulation of FA metabolism. On the other hand, PPAR β/δ activation regulates FA metabolism and lactate production ensuring simultaneously the energetic metabolism for SC and germ cells.
authors.affiliation: Centro de Investigaciones Endocrinólogicas "Dr Cesar Bergadá" (CEDIE-CONICET), Hospital de Niños "R. Gutiérrez", Gallo 1330, C1425EDF Buenos Aires, Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Regueira
authors.affiliation: Centro de Investigaciones Endocrinólogicas "Dr Cesar Bergadá" (CEDIE-CONICET), Hospital de Niños "R. Gutiérrez", Gallo 1330, C1425EDF Buenos Aires, Argentina.
authors.forename: M F
authors.initials: MF
authors.lastname: Riera
authors.affiliation: Centro de Investigaciones Endocrinólogicas "Dr Cesar Bergadá" (CEDIE-CONICET), Hospital de Niños "R. Gutiérrez", Gallo 1330, C1425EDF Buenos Aires, Argentina.
authors.forename: M N
authors.initials: MN
authors.lastname: Galardo
authors.affiliation: Centro de Investigaciones Endocrinólogicas "Dr Cesar Bergadá" (CEDIE-CONICET), Hospital de Niños "R. Gutiérrez", Gallo 1330, C1425EDF Buenos Aires, Argentina.
authors.forename: E H
authors.initials: EH
authors.lastname: Pellizzari
authors.affiliation: Centro de Investigaciones Endocrinólogicas "Dr Cesar Bergadá" (CEDIE-CONICET), Hospital de Niños "R. Gutiérrez", Gallo 1330, C1425EDF Buenos Aires, Argentina.
authors.forename: S B
authors.initials: SB
authors.lastname: Cigorraga
authors.affiliation: Centro de Investigaciones Endocrinólogicas "Dr Cesar Bergadá" (CEDIE-CONICET), Hospital de Niños "R. Gutiérrez", Gallo 1330, C1425EDF Buenos Aires, Argentina. Electronic address: smeroni@cedie.org.ar.
authors.forename: S B
authors.initials: SB
authors.lastname: Meroni
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D042964:Acyl-CoA Dehydrogenase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D018955:CD36 Antigens / Q000378:metabolism; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008297:Male; D008954:Models, Biological; D047493:PPAR alpha / Q000378:metabolism*; D047494:PPAR delta / Q000378:metabolism*; D048068:PPAR-beta / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D011743:Pyrimidines / Q000494:pharmacology; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D013844:Thiazoles / Q000494:pharmacology; D010636:Phenols; D013438:Sulfhydryl Compounds
chemical_list: D000103:Acetyl-CoA Carboxylase; D042964:Acyl-CoA Dehydrogenase; D018955:CD36 Antigens; D002334:Carnitine O-Palmitoyltransferase; D005227:Fatty Acids; D005947:Glucose; D007770:L-Lactate Dehydrogenase; D007773:Lactates; D047493:PPAR alpha; D047494:PPAR delta; D048068:PPAR-beta; D011743:Pyrimidines; D011768:Pyruvate Dehydrogenase Complex; D012333:RNA, Messenger; D013844:Thiazoles; C479979:(4-(((2-(3-fluoro-4-(trifluoromethyl)phenyl)-4-methyl-1,3-thiazol-5-yl)methyl)sulfanyl)-2-methylphenoxy)acetic acid; D010636:Phenols; D013438:Sulfhydryl Compounds; C006253:pirinixic acid
keywords: Fatty acid oxidation; Lactate; Pyruvate Dehydrogenase Complex; Testis