title: Expression stabilities of candidate reference genes for RT-qPCR under different stress conditions in soybean.
abstract: Due to its accuracy, sensitivity and high throughput, real time quantitative PCR (RT-qPCR) has been widely used in analysing gene expression. The quality of data from such analyses is affected by the quality of reference genes used. Expression stabilities for nine candidate reference genes widely used in soybean were evaluated under different stresses in this study. Our results showed that EF1A and ACT11 were the best under salinity stress, TUB4, TUA5 and EF1A were the best under drought stress, ACT11 and UKN2 were the best under dark treatment, and EF1B and UKN2 were the best under virus infection. EF1B and UKN2 were the top two genes which can be reliably used in all of the stress conditions assessed.
authors.affiliation: College of Life Science, Agricultural University of Hebei, Baoding, Hebei Province, China.
authors.forename: Shuhua
authors.initials: S
authors.lastname: Ma
authors.forename: Hongwei
authors.initials: H
authors.lastname: Niu
authors.forename: Chunji
authors.initials: C
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Chunyan
authors.initials: C
authors.lastname: Hou
authors.forename: Dongmei
authors.initials: D
authors.lastname: Wang
mesh_terms: D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013025:Glycine max / Q000235:genetics*
chemical_list: D010940:Plant Proteins