title: Cryo-electron tomography: moving towards revealing the viral life cycle of Rice dwarf virus.
abstract: It is well known that viruses utilize the host cellular systems for their infection and replication processes. However, the molecular mechanisms underlying these processes are poorly understood for most viruses. To understand these molecular mechanisms, it is essential to observe the viral and virus-related structures and analyse their molecular interactions within a cellular context. Cryo-electron microscopy and tomography offer the potential to observe macromolecular structures and to analyse their molecular interactions within the cell. Here, using cryo-electron microscopy and tomography, the structures of Rice dwarf virus are reported within fully hydrated insect vector cells grown on electron microscopy grids towards revealing the viral infection and replication mechanisms.
authors.affiliation: National Institute for Physiological Sciences, 38 Nishigonaka, Myodaiji, Okazaki, Aichi 444-8585, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Miyazaki
authors.forename: Fusamichi
authors.initials: F
authors.lastname: Akita
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nakagawa
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Murata
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Omura
authors.forename: Kenji
authors.initials: K
authors.lastname: Iwasaki
mesh_terms: D055032:Electron Microscope Tomography / Q000379:methods*; D012087:Reoviridae / Q000502:physiology*
keywords: Phytoreovirus; Rice dwarf virus; cryo-electron microscopy; cryo-electron tomography; virus structure