title: Hollow polydimethylsiloxane beads with a porous structure for cell encapsulation.
abstract: Based on a water-in-oil-in-water emulsion system, porous and hollow polydimethylsiloxane (PDMS) beads containing cells using a simple fluidic device with three flow channels are fabricated. Poly(ethylene glycol) (PEG) in the PDMS oil phase is served as a porogen for pore development. The feasibility of the porous PDMS beads prepared with different PEG concentrations (10, 20, and 30 wt%) for cell encapsulation in terms of pore size, protein diffusion, and cell proliferation inside the PDMS beads is evaluated. The PDMS beads prepared with PEG 30 wt% are exhibited a highly porous structure and facilitated fast diffusion of protein from the core domain to the outer phase, eventually leading to enhanced cell proliferation. The results clearly indicate that hollow PDMS beads with a porous structure could provide a favorable microenvironment for cell survival due to the large porous structure.
authors.affiliation: Department of Biotechnology, The Catholic University of Korea, Gyeonggi-do, Republic of Korea.
authors.forename: Myeong-Jin
authors.initials: MJ
authors.lastname: Oh
authors.forename: Tae-Kyoung
authors.initials: TK
authors.lastname: Ryu
authors.forename: S-W
authors.initials: SW
authors.lastname: Choi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D002417:Cattle; D049109:Cell Proliferation; D002470:Cell Survival; D004058:Diffusion; D004129:Dimethylpolysiloxanes / Q000138:chemical synthesis / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology*; D051379:Mice; D015394:Molecular Structure; D041681:NIH 3T3 Cells; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D016062:Porosity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; C013830:baysilon
keywords: PDMS; cell encapsulation; fluidic device; hollow core; porous wall