title: α2δ-1 gene deletion affects somatosensory neuron function and delays mechanical hypersensitivity in response to peripheral nerve damage.
abstract: The α2δ-1 subunit of voltage-gated calcium channels is upregulated after sensory nerve injury and is also the therapeutic target of gabapentinoid drugs. It is therefore likely to play a key role in the development of neuropathic pain. In this study, we have examined mice in which α2δ-1 gene expression is disrupted, to determine whether α2δ-1 is involved in various modalities of nociception, and for the development of behavioral hypersensitivity after partial sciatic nerve ligation (PSNL). We find that naive α2δ-1(-/-) mice show a marked behavioral deficit in mechanical and cold sensitivity, but no change in thermal nociception threshold. The lower mechanical sensitivity is mirrored by a reduced in vivo electrophysiological response of dorsal horn wide dynamic range neurons. The CaV2.2 level is reduced in brain and spinal cord synaptosomes from α2δ-1(-/-) mice, and α2δ-1(-/-) DRG neurons exhibit lower calcium channel current density. Furthermore, a significantly smaller number of DRG neurons respond to the TRPM8 agonist menthol. After PSNL, α2δ-1(-/-) mice show delayed mechanical hypersensitivity, which only develops at 11 d after surgery, whereas in wild-type littermates it is maximal at the earliest time point measured (3 d). There is no compensatory upregulation of α2δ-2 or α2δ-3 after PSNL in α2δ-1(-/-) mice, and other transcripts, including neuropeptide Y and activating transcription factor-3, are upregulated normally. Furthermore, the ability of pregabalin to alleviate mechanical hypersensitivity is lost in PSNL α2δ-1(-/-) mice. Thus, α2δ-1 is essential for rapid development of mechanical hypersensitivity in a nerve injury model of neuropathic pain.
authors.affiliation: Department of Neuroscience, Physiology and Pharmacology, University College London, London, WC1E 6BT, United Kingdom, Nuffield Department of Clinical Neurosciences, John Radcliffe Hospital, University of Oxford, Oxford, OX1 2JD, United Kingdom, and University of Cincinnati, College of Medicine, Cincinnati, Ohio 45229.
authors.forename: Ryan
authors.initials: R
authors.lastname: Patel
authors.forename: Claudia S
authors.initials: CS
authors.lastname: Bauer
authors.forename: Manuela
authors.initials: M
authors.lastname: Nieto-Rostro
authors.forename: Wojciech
authors.initials: W
authors.lastname: Margas
authors.forename: Laurent
authors.initials: L
authors.lastname: Ferron
authors.forename: Kanchan
authors.initials: K
authors.lastname: Chaggar
authors.forename: Kasumi
authors.initials: K
authors.lastname: Crews
authors.forename: Juan D
authors.initials: JD
authors.lastname: Ramirez
authors.forename: David L H
authors.initials: DL
authors.lastname: Bennett
authors.forename: Arnold
authors.initials: A
authors.lastname: Schwartz
authors.forename: Anthony H
authors.initials: AH
authors.lastname: Dickenson
authors.forename: Annette C
authors.initials: AC
authors.lastname: Dolphin
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000235:genetics* / Q000378:metabolism; D003080:Cold Temperature; D005727:Ganglia, Spinal / Q000378:metabolism / Q000503:physiopathology; D006930:Hyperalgesia / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D051379:Mice; D018345:Mice, Knockout; D009437:Neuralgia / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D017288:Pain Threshold / Q000502:physiology; D059348:Peripheral Nerve Injuries / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D010812:Physical Stimulation; D012584:Sciatic Nerve / Q000293:injuries / Q000378:metabolism / Q000503:physiopathology; D011984:Sensory Receptor Cells / Q000502:physiology*
chemical_list: D015220:Calcium Channels