title: A potential cellular host factor homologous to ZNF-136 can interact with truncated nonstructural protein 2 of hepatitis C virus.
abstract: The cytoplasmic portion of the NS2 protein of hepatitis C virus (HCV) fused with a thioredoxin (TrX)-His tag was expressed in BL21 (DE3) cells and purified using an Ni-NTA His resin. Using a pull-down assay, a specific protein from the cell lysate of HepG2 cell lines was isolated together with immobilized truncated NS2 protein using Ni-NTA resin. Through peptide mass fingerprinting, this specific protein was found to be homologous to a repressor of transcription, ZNF136.
authors.affiliation: State key laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan, 430071, China, cliu1234@umd.edu.
authors.forename: Chaohong
authors.initials: C
authors.lastname: Liu
authors.forename: Xinwen
authors.initials: X
authors.lastname: Chen
mesh_terms: D004268:DNA-Binding Proteins / Q000378:metabolism*; D056945:Hep G2 Cells; D016174:Hepacivirus / Q000235:genetics / Q000502:physiology*; D022781:Hepatocytes / Q000821:virology; D054884:Host-Pathogen Interactions; D006801:Humans; D013058:Mass Spectrometry; D011485:Protein Binding; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; C097010:NS2 protein, Hepatitis C virus; D017361:Viral Nonstructural Proteins; C095225:ZNF136 protein, human