title: Maillard reaction products as antimicrobial components for packaging films.
abstract: Active packaging foils with incorporated antimicrobial agents release the active ingredient during food storage. Maillard reaction products (MRPs) show antimicrobial activity that is at least partially mediated by H2O2. De novo generation of H2O2 by an MRP fraction, extracted from a ribose/lysine Maillard reaction mixture by 85% ethanol, was monitored at three concentrations (1.6, 16.1, and 32.3g/L) and three temperatures (4, 25, and 37 °C) between 0 and 96 h, reaching a maximum of 335 μM H2O2 (32.3g/L, 37 °C, 96 h). The active MRP fraction (16.1g/L) completely inhibited the growth of Escherichia coli for 24h and was therefore incorporated in a polyvinyl acetate-based lacquer and dispersed onto a low-density polyethylene film. The coated film generated about 100 μM H2O2 and resulted in a log-reduction of >5 log-cycles against E. coli. Thus, MRPs can be considered as active ingredients for antimicrobial packaging materials.
authors.affiliation: Fraunhofer Institute for Process Engineering and Packaging (IVV), Giggenhauser Strasse 35, 85354 Freising, Germany; Department of Chemistry and Pharmacy, Food Chemistry, Emil Fischer Center, Friedrich-Alexander-University of Erlangen-Nuremberg, Schuhstrasse 19, 91052 Erlangen, Germany.
authors.forename: Carolin
authors.initials: C
authors.lastname: Hauser
authors.forename: Ulla
authors.initials: U
authors.lastname: Müller
authors.forename: Tanja
authors.initials: T
authors.lastname: Sauer
authors.forename: Kerstin
authors.initials: K
authors.lastname: Augner
authors.forename: Monika
authors.initials: M
authors.lastname: Pischetsrieder
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects*; D000431:Ethanol / Q000737:chemistry; D018857:Food Packaging; D005519:Food Preservation / Q000379:methods*; D006861:Hydrogen Peroxide / Q000737:chemistry; D015416:Maillard Reaction; D013696:Temperature; D013997:Time Factors
chemical_list: D000890:Anti-Infective Agents; D000431:Ethanol; D006861:Hydrogen Peroxide
keywords: Active packaging; Antimicrobial films; FOX; Hydrogen peroxide; LDPE; MRM; MRP; Maillard reaction mixture; Maillard reaction product; Maillard reaction products; PBS; PCA; Preservation; cfu; colony forming units; dm; dry matter; ferrous oxidation xylenol orange; low density polyethylene; perchloric acid; phosphate-buffered saline