title: An S-domain receptor-like kinase, OsSIK2, confers abiotic stress tolerance and delays dark-induced leaf senescence in rice.
abstract: Receptor-like kinases play important roles in plant development and defense responses; however, their functions in other processes remain unclear. Here, we report that OsSIK2, an S-domain receptor-like kinase from rice (Oryza sativa), is involved in abiotic stress and the senescence process. OsSIK2 is a plasma membrane-localized protein with kinase activity in the presence of Mn(2+). OsSIK2 is expressed mainly in rice leaf and sheath and can be induced by NaCl, drought, cold, dark, and abscisic acid treatment. Transgenic plants overexpressing OsSIK2 and mutant sik2 exhibit enhanced and reduced tolerance to salt and drought stress, respectively, compared with the controls. Interestingly, a truncated version of OsSIK2 without most of the extracellular region confers higher salt tolerance than the full-length OsSIK2, likely through the activation of different sets of downstream genes. Moreover, seedlings of OsSIK2-overexpressing transgenic plants exhibit early leaf development and a delayed dark-induced senescence phenotype, while mutant sik2 shows the opposite phenotype. The downstream PR-related genes specifically up-regulated by full-length OsSIK2 or the DREB-like genes solely enhanced by truncated OsSIK2 are all induced by salt, drought, and dark treatments. These results indicate that OsSIK2 may integrate stress signals into a developmental program for better adaptive growth under unfavorable conditions. Manipulation of OsSIK2 should facilitate the improvement of production in rice and other crops.
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Chen
authors.forename: Hada
authors.initials: H
authors.lastname: Wuriyanghan
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Zhang
authors.forename: Kai-Xuan
authors.initials: KX
authors.lastname: Duan
authors.forename: Hao-Wei
authors.initials: HW
authors.lastname: Chen
authors.forename: Qing-Tian
authors.initials: QT
authors.lastname: Li
authors.forename: Xiang
authors.initials: X
authors.lastname: Lu
authors.forename: Si-Jie
authors.initials: SJ
authors.lastname: He
authors.forename: Biao
authors.initials: B
authors.lastname: Ma
authors.forename: Wan-Ke
authors.initials: WK
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Lin
authors.forename: Shou-Yi
authors.initials: SY
authors.lastname: Chen
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Zhang
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics; D000975:Antioxidants / Q000378:metabolism; D003624:Darkness; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D010641:Phenotype; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport / Q000187:drug effects; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology
chemical_list: D000975:Antioxidants; D010940:Plant Proteins; D012965:Sodium Chloride; D011494:Protein Kinases