title: A Comparative Study of the Photosensitizer Penetration into Artificial Caries Lesions in Dentin Measured by the Confocal Raman Microscopy.
abstract: This study utilized the confocal Raman microspectroscopy (CRM) technique for the first time to investigate the degree of the penetration of toluidine blue-orto (TBO) in artificial caries lesions produced by two distinct caries-inducing models. The dentin specimens (n = 10) were divided into three groups: control, in vitro and in situ. Thereafter, the lesion depth and the demineralization level were evaluated by cross-sectional microhardness (CSMH). CRM mapping across the dentin surface was assessed after the dye application. The CSMH and CRM data were analyzed by t-test and ANOVA, respectively (P < 0.05). The values of the lesion depth and the demineralization areas were higher for in situ samples (P < 0.05). The TBO penetration values (μm) for the control, in vitro and in situ groups were 44.8 ± 5.6, 46.1 ± 4.5 and 51.2 ± 8.5, respectively. There were no statistically significant differences among the groups (P > 0.05). The rate of TBO penetration was detected up to about <50 μm and the demineralization level did not influence the results. These results have showed promising parameters to develop new protocols for deep caries lesions management using photodynamic antimicrobial chemotherapy.
authors.affiliation: Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, Maryland.
authors.forename: Mary Anne S
authors.initials: MA
authors.lastname: Melo
authors.affiliation: Post-graduation Program, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Juliana P M L
authors.initials: JP
authors.lastname: Rolim
authors.affiliation: Post-graduation Program, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Iriana C J
authors.initials: IC
authors.lastname: Zanin
authors.affiliation: Physics Department, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: José J A
authors.initials: JJ
authors.lastname: Silva
authors.affiliation: Physics Department, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Alexandre R
authors.initials: AR
authors.lastname: Paschoal
authors.affiliation: Physics Department, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Alejandro P
authors.initials: AP
authors.lastname: Ayala
authors.affiliation: Post-graduation Program, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Lidiany K A
authors.initials: LK
authors.lastname: Rodrigues
mesh_terms: D003731:Dental Caries / Q000378:metabolism*; D003804:Dentin / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D018613:Microscopy, Confocal; D008954:Models, Biological; D017319:Photosensitizing Agents / Q000378:metabolism*
chemical_list: D017319:Photosensitizing Agents