title: Monitoring receptor oligomerization by line-scan fluorescence cross-correlation spectroscopy.
abstract: Membrane-localized receptor proteins are involved in many signaling cascades, and diffusion and oligomerization are key processes controlling their activity. In order to study these processes in living cells, fluorescence fluctuation spectroscopy techniques have been developed that allow the quantification of concentration levels, diffusion rates, and interactions between fluorescently labeled receptor proteins at the nanomolar concentration level. This chapter presents a brief introduction to the technique and a protocol to measure and quantify the diffusion and oligomerization of human histamine1 receptor complexes in living HeLa cells using line-scanning fluorescence cross-correlation spectroscopy.
authors.affiliation: Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy (LCAM), Swammerdam Institute for Life Sciences (SILS), University of Amsterdam, The Netherlands.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Hink
authors.forename: Marten
authors.initials: M
authors.lastname: Postma
mesh_terms: D004058:Diffusion; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D057054:Molecular Imaging; D017785:Photons; D055503:Protein Multimerization; D011969:Receptors, Histamine H1 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013050:Spectrometry, Fluorescence / Q000379:methods / Q000706:statistics & numerical data*; D013194:Staining and Labeling; D014162:Transfection
chemical_list: C485182:Cyan Fluorescent Protein; D011969:Receptors, Histamine H1; D049452:Green Fluorescent Proteins
keywords: Dimerization; Fluorescence cross-correlation spectroscopy; Human histamine1 receptor; Line-scan FCCS