title: Detection and quantification of lysine acetyl-alteration using antibody microarray.
abstract: BACKGROUND
  Lysine acetylation is a reversible and dynamic post-translational modification on proteins, and plays an important role in diverse biological processes. Technological limitations have so far prevented comparative quantification of lysine acetylation in different samples.
  
  
  RESULTS
  We developed a method to efficiently study lysine acetylation on individual proteins from complex mixtures, using antibody microarrays to capture individual proteins followed by detection with lysine acetyl antibody. By profiling both protein and acetylation variations in multiple samples using this microarray, we found cancer-associated lysine acetylation alteration on VEGF in the serum of hepatocellular carcinoma patients.
  
  
  CONCLUSIONS
  Microarrays of lysine acetylation are highly effective for detecting acetylation, and should be useful in identifying and validating disease-associated acetylation alterations as biomarkers under both normal and pathological circumstances.
authors.affiliation: Department of Chemistry, Fudan University, Shanghai 200433, China.
authors.forename: Feina
authors.initials: F
authors.lastname: Yao
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
authors.forename: Yinkun
authors.initials: Y
authors.lastname: Liu
authors.forename: Huizhi
authors.initials: H
authors.lastname: Fan
mesh_terms: D000107:Acetylation; D000818:Animals; D000906:Antibodies / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D001710:Biotin / Q000737:chemistry; D006528:Carcinoma, Hepatocellular / Q000097:blood* / Q000175:diagnosis / Q000235:genetics; D002417:Cattle; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000235:genetics; D008239:Lysine / Q000378:metabolism; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011499:Protein Processing, Post-Translational; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry; D042461:Vascular Endothelial Growth Factor A / Q000097:blood / Q000235:genetics*
chemical_list: D000906:Antibodies; D014408:Biomarkers, Tumor; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D012710:Serum Albumin, Bovine; D001710:Biotin; D019809:Streptavidin; D008239:Lysine