title: Recombinant adenovirus snake venom cystatin inhibits the growth, invasion, and metastasis of B16F10 cells in vitro and in vivo.
abstract: Previous studies have shown that transfection of the snake venom cystatin (sv-cystatin) gene can inhibit the invasion and metastasis of tumor cells. The aim of this study was to investigate the pharmaceutical applications of sv-cystatin in melanoma gene therapy. We constructed a recombinant adenovirus carrying sv-cystatin (Ad/sv-cystatin) and a control virus (Ad/null). Matrigel assays were used to assess melanoma cell migration and invasiveness in vitro. The antimelanoma effects of Ad/sv-cystatin were assessed in a syngeneic mouse model with an experimental lung colonization assay. Ad/sv-cystatin significantly inhibited the invasion and growth of B16F10 cells in vitro compared with control and Ad/null. Ad/sv-cystatin significantly inhibited experimental lung colonization in C57BL/6 mice as compared with that in control (P<0.001) and Ad/null-treated mice (P<0.001), with an inhibition rate of 51 and 46%, respectively. Ad/sv-cystatin slowed the increase in lung weight in C57BL/6 mice as compared with that in control mice (P<0.001) and Ad/null-treated mice (P<0.001), with an inhibition rate of 40 and 35%, respectively. Our results indicate that Ad/sv-cystatin suppresses mouse melanoma invasion, metastasis, and growth in vitro and in vivo. Our findings provide support for the further examination of the pharmaceutical applications of Ad/sv-cystatin.
authors.affiliation: aKey Laboratory of Ministry of Education for Gastrointestinal Cancer, Research Center of Molecular Medicine bHepatobiliary Surgery Institute, Union Hospital, Fujian Medical University, Fuzhou cSchool of Basic Medical Science, Putian University, Putian, Fujian, People's Republic of China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Xie
authors.forename: Nanhong
authors.initials: N
authors.lastname: Tang
authors.forename: Yangyuan
authors.initials: Y
authors.lastname: Lin
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Wang
authors.forename: Xu
authors.initials: X
authors.lastname: Lin
authors.forename: Jianyin
authors.initials: J
authors.lastname: Lin
mesh_terms: D000256:Adenoviridae / Q000378:metabolism; D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen; D015891:Cystatins / Q000494:pharmacology*; D004338:Drug Combinations; D015316:Genetic Therapy / Q000379:methods; D007797:Laminin; D008297:Male; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis / Q000188:drug therapy; D009368:Neoplasm Transplantation; D011509:Proteoglycans; D011994:Recombinant Proteins / Q000494:pharmacology; D012910:Snake Venoms / Q000737:chemistry*; D014162:Transfection
chemical_list: D015891:Cystatins; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D011994:Recombinant Proteins; D012910:Snake Venoms; C059485:matrigel; D003094:Collagen