title: Identification and quantification of 5,6,7,8-tetrahydro-2-(2-phenylethyl)chromones in Chinese eaglewood by HPLC with diode array detection and MS.
abstract: A sensitive and reliable HPLC coupled with diode array detection and MS method was developed and validated for the first time to simultaneously identify and quantify eight characteristic 5,6,7,8-tetrahydro-2-(2-phenylethyl)chromones (THPECs) in Chinese eaglewood. Chromatographic separation was performed on a Zorbax SB C18 column with a gradient of acetonitrile/0.1% formic acid/water as the mobile phase. The MS fragmentation behavior of THPECs was characterized as the successive neutral loss of two molecules of H2 O ([M+H-18-18](+) ) and then two molecules of CO ([M+H-18-18-28-28](+) ), which could be used to differentiate Chinese eaglewood from counterfeits. Validation of the developed analytical method showed good linearity, satisfactory precision, and good recovery. The established method was successfully applied to the simultaneous determination of eight THPECs in ten batches of Chinese eaglewood, which could be used as a tool for the quality control of Chinese eaglewood.
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Tu
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D013058:Mass Spectrometry; D015394:Molecular Structure; D011786:Quality Control; D015203:Reproducibility of Results; D029645:Thymelaeaceae / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C062550:2-(2-phenylethyl)chromone
keywords: Aquilaria sinensis; Chinese eaglewood; Chromones; Quality control