title: Genetic characterisation of infectious bursal disease virus isolates in Ethiopia.
abstract: The objective of the investigation was to characterise infectious bursal disease viruses (IBDV) circulating in commercial and breeding poultry farms in Ethiopia between 2009 and 2011. The nucleotide and deduced amino acid sequence for VP2 hypervariable region of ten IBDVs were determined by RT-PCR, sequenced and compared to well characterised IBDV isolates worldwide. IBDV genetic material was amplified directly from bursa or cell passaged material. Phylogenetically, Ethiopian IBDVs represented two genetic lineages: very virulent (vv) IBDVs or variants of the classical attenuated vaccine strain (D78). The nucleotide identity between Ethiopian vvIBDVs ranged between 0% and 2.6%. Ethiopian vvIBDVs are clustered phylogenetically with the African IBDV genetic lineage, independent of the Asian/European lineage. This report demonstrates the circulation of vvIBDV in commercial and breeding poultry farms in Ethiopia.
authors.affiliation: National Veterinary Institute, Debre-Zeit, Ethiopia.
authors.forename: Shiferaw
authors.initials: S
authors.lastname: Jenberie
authors.affiliation: The Institute of Infection and Global Health, University of Liverpool, Leahurst Campus, CH64 7TE, United Kingdom; International Livestock Research Institute, Addis Ababa, Ethiopia.
authors.forename: Stacey E
authors.initials: SE
authors.lastname: Lynch
authors.affiliation: National Veterinary Institute, Debre-Zeit, Ethiopia.
authors.forename: Fekadu
authors.initials: F
authors.lastname: Kebede
authors.affiliation: The Institute of Infection and Global Health, University of Liverpool, Leahurst Campus, CH64 7TE, United Kingdom.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Christley
authors.affiliation: National Veterinary Institute, Debre-Zeit, Ethiopia.
authors.forename: Esayas
authors.initials: E
authors.lastname: Gelaye
authors.affiliation: College of Veterinary Medicine and Agriculture, Addis Ababa University, Debre-Zeit, Ethiopia.
authors.forename: Haileleul
authors.initials: H
authors.lastname: Negussie
authors.affiliation: Hawassa University School of Veterinary Medicine, P.O. Box 05, Hawassa, Ethiopia; Norwegian School of Veterinary Science, Department of Food Safety and Infection Biology, Centre for Epidemiology and Biostatistics, P.O. Box 8146 Dep., 0033 Oslo, Norway. Electronic address: ka7588@yahoo.com.
authors.forename: Kassahun
authors.initials: K
authors.lastname: Asmare
authors.affiliation: National Veterinary Institute, Debre-Zeit, Ethiopia; Norwegian School of Veterinary Science, Department of Food Safety and Infection Biology, Centre for Epidemiology and Biostatistics, P.O. Box 8146 Dep., 0033 Oslo, Norway.
authors.forename: Gelagay
authors.initials: G
authors.lastname: Ayelet
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000662:veterinary / Q000821:virology; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D002645:Chickens; D005002:Ethiopia; D007243:Infectious bursal disease virus / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology
chemical_list: D036022:Capsid Proteins
keywords: Infectious bursal disease; Phylogenetic; RT-PCR; Sequencing; VP2; vvIBDV