title: Whole-transcriptome analysis of hepatocellular carcinoma.
abstract: Hepatocellular carcinoma (HCC) is one of the leading causes of death in the world. It involves a complicated pathogenesis. In the present study, we tried to uncover the underlying mechanisms from the RNA level. Whole-transcriptome analysis using RNA-seq data showed that the tumor cells had higher expression levels of genes compared to normal tissue cells, with much more genes related to metabolism, synthesis, and transportation. A total of 4,638 differentially expressed genes were obtained in HCC, in which 3,476 were up-regulated and 1,162 were down-regulated. A total of 2,367 new isoforms and 46 fusion genes were detected in HCC cells using computational method. Within these variants, new isoforms of NIR and fusion genes PARP15-AHSG caught our attention for their potential roles in cancer development. These findings could be beneficial in guiding future researches.
authors.affiliation: Department of Gastroenterology and Hepatology, Shengjing Hospital of China Medical University, Shenyang, 110004, China, audreylin73@hotmail.com.
authors.forename: Lianjie
authors.initials: L
authors.lastname: Lin
authors.forename: Dongxu
authors.initials: D
authors.lastname: Wang
authors.forename: Nan
authors.initials: N
authors.lastname: Cao
authors.forename: Yan
authors.initials: Y
authors.lastname: Lin
authors.forename: Yu
authors.initials: Y
authors.lastname: Jin
authors.forename: Changqing
authors.initials: C
authors.lastname: Zheng
mesh_terms: D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics
chemical_list: D012313:RNA