title: Molecular pathological study of the human nasopharyngeal carcinoma CNE3 cell line.
abstract: The present study aimed to identify the molecular pathological changes of the nasopharyngeal carcinoma (NPC) epithelial CNE3 cell line, which has been used in experimental studies for 20 years in a culture environment. The pathological type of NPC and the presence of the Epstein-Barr virus (EBV) were identified. CNE3 short tandem repeats (STRs) were amplified, analyzed and compared using metastatic carcinoma tissue from primary NPC. Immunohistochemistry (IHC) andin situhybridization (ISH) were used to identify the immunophenotype and EBV-encoded small RNA (EBER) expression in nude mice transplanted CNE3 tumor cells. Polymerase chain reaction (PCR) and DNA sequencing were used to identify the EBV oncogene,BamH1-A right frame 1 (BARF1) and electron microscopy was used to analyze the organization of the ultrastructure. CNE3 was not cross-contaminated by other human cell lines and the EBV was no longer present in the CNE3 cells. The pathological type of CNE3 was transformed from an undifferentiated non-keratinizing carcinoma with focal adenocarcinoma differentiation into a poorly-differentiated adenocarcinoma. In conclusion, this knowledge on the molecular pathological changes of CNE3 may aid in the development of new research approaches for NPC.
authors.affiliation: Research Center of Medical Sciences, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Jiao
authors.forename: Xiang-Lan
authors.initials: XL
authors.lastname: Mo
authors.forename: Jiao
authors.initials: J
authors.lastname: Lan
authors.forename: Rui-Ping
authors.initials: RP
authors.lastname: Xiao
authors.forename: Xiang-Zhen
authors.initials: XZ
authors.lastname: Zhou
authors.forename: Zhen-Lu
authors.initials: ZL
authors.lastname: Huang
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Mo
authors.forename: Gang
authors.initials: G
authors.lastname: Li
keywords: CNE3; molecular pathology; nasopharyngeal carcinoma