title: Analysis of free and bound phenolics in wine and grapes by GC-MS after automated SPE.
abstract: The results of validation of a method for the analysis of free and bound phenolics in wine and grapes are presented. Wine and grape extracts are fractionated by automated solid-phase extraction on Bond Elut PPL cartridges to give free and bound phenolic fractions. Bound fractions are subjected to acid hydrolysis, and the phenolics released are recovered by solid-phase extraction on Bond Elut PPL cartridges. The fractions are further purified by automated solid-phase extraction on Bond Elut silica cartridges. After derivatisation to form trimethylsilyl ethers, the phenolics are determined by gas chromatography-mass spectrometry with selected ion monitoring. The method is suitable for robust, high-throughput monitoring of the concentrations of phenolics that can affect the palatability of wine.
authors.affiliation: Department of Environment and Primary Industries, Ernest Jones Drive, Macleod, 3085, Australia, David.Allen@depi.vic.gov.au.
authors.forename: David
authors.initials: D
authors.lastname: Allen
authors.forename: Anh Duyen
authors.initials: AD
authors.lastname: Bui
authors.forename: Nicole
authors.initials: N
authors.lastname: Cain
authors.forename: Gavin
authors.initials: G
authors.lastname: Rose
authors.forename: Mark
authors.initials: M
authors.lastname: Downey
mesh_terms: D001331:Automation; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D010636:Phenols / Q000032:analysis*; D052616:Solid Phase Extraction; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
chemical_list: D010636:Phenols