title: The target gene of tae-miR164, a novel NAC transcription factor from the NAM subfamily, negatively regulates resistance of wheat to stripe rust.
abstract: microRNA (miRNA) participates in various physiological and biochemical processes in plants by regulating corresponding target genes. NAC [NAM (no apical meristem), ATAF (Arabidopsis transcription activation factor) and CUC (cup-shaped cotyledon)] transcription factors, usually as the targets of miR164, play important roles in the regulation of plant development and responses to abiotic and biotic stresses. In a previous study, the target gene of tae-miR164 in wheat was sequenced through degradome sequencing. In this study, we isolated the full-length cDNA of the candidate target gene, which is a NAC transcription factor gene in the NAM subfamily, and designated it as TaNAC21/22 after bioinformatics analysis. The interaction between TaNAC21/22 and tae-miR164 was confirmed experimentally through co-transformation of both genes in tobacco leaves. Transcript accumulation of TaNAC21/22 and tae-miR164 showed contrasting divergent expression patterns in wheat response to Puccinia striiformis f. sp. tritici (Pst). TaNAC21/22 was confirmed to be located in the nucleus and could function as a transcriptional activator. Silencing of the individual gene showed that TaNAC21/22 negatively regulates resistance to stripe rust. These results indicate that the target of tae-miR164, a novel NAC transcription factor from the NAM subfamily of wheat, plays an important role in regulating the resistance of host plants to stripe rust.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Feng
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Duan
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhang
authors.forename: Xiaorui
authors.initials: X
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Lili
authors.initials: L
authors.lastname: Huang
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Wang
authors.forename: Zhensheng
authors.initials: Z
authors.lastname: Kang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001487:Basidiomycota / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D060467:Disease Resistance / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques; D017343:Genes, Plant; D035683:MicroRNAs; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D018515:Plant Leaves / Q000166:cytology / Q000382:microbiology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation; D014170:Transformation, Genetic; D014908:Triticum / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*
chemical_list: D018076:DNA, Complementary; D035683:MicroRNAs; D010940:Plant Proteins; D014157:Transcription Factors