title: Estimation of prostaglandin E2 levels in gingival crevicular fluid in periodontal health, disease and after treatment.
abstract: OBJECTIVE
  Initial research has shown a positive correlation between the severity of periodontal disease (PD) and prostaglandin E2 (PGE2) concentrations in gingival crevicular fluid (GCF). However, there are no enough reports to correlate the PGE2 concentrations in GCF in periodontal health, disease and after treatment. Hence, the present study is aimed to estimate the levels of PGE2 in GCF in periodontal health, disease and after periodontal therapy.
  
  
  METHODS
  A periodontal examination and collection of GCF by extracrevicular method was performed in 25 subjects selected randomly and categorized into three groups on the basis of plaque index, gingival index, probing pocket depth and clinical attachment level. Group I (healthy) consists of 10 subjects, Group II (chronic periodontitis) consists of 15 patients and Group III (after treatment group) consists of 15 patients of Group II. PGE2 levels were estimated in GCF samples by using the enzyme linked immunosorbent assay.
  
  
  RESULTS
  All clinical parameters improved significantly after therapy (P < 0.001). PGE2 was detected in all the samples. Highest mean PGE2 concentrations in GCF were obtained for Group II while the lowest concentrations were seen in Group I and Group III. Statistically significant difference was found between the levels of PGE2 at Group-II and Group-III (P < 0.05).
  
  
  CONCLUSIONS
  There is a substantial increase in the concentrations of PGE2 as PD progresses. Since PGE2 levels in GCF are positively correlated with gingival index, plaque index, probing pocket depths and clinical attachment levels, PGE2 may be considered as a Novel Biomarker in PD progression. However, controlled, longitudinal studies are needed to confirm this possibility.
authors.affiliation: Department of Periodontics and Implantology, CKS Teja Institute of Dental Sciences and Research, Tirupati, India.
authors.forename: A Kishore
authors.initials: AK
authors.lastname: Kumar
authors.forename: N Ravindra
authors.initials: NR
authors.lastname: Reddy
authors.forename: Madhu
authors.initials: M
authors.lastname: Babu
authors.forename: P Mohan
authors.initials: PM
authors.lastname: Kumar
authors.forename: Venkat Subba
authors.initials: VS
authors.lastname: Reddy
authors.forename: C Vinay
authors.initials: CV
authors.lastname: Chavan
keywords: Gingival crevicular fluid; periodontal disease; prostaglandin E2; root planing; scaling