title: Both expression of cytokines and posterior annulus fibrosus rupture are essential for pain behavior changes induced by degenerative intervertebral disc: An experimental study in rats.
abstract: The aim of this study was to analyze the relationship between intervertebral disc degeneration and low back pain (LBP). Rat L4/5 disc degeneration model was established by annular puncture using a 0.4 mm needle anteriorly or posteriorly. In both anterior and posterior puncture models, magnetic resonance imaging (MRI) and histological analyses revealed marked disc degeneration 2 weeks after puncture. Cytokine expression was up-regulated in different level in nucleus pulposus (NP) from 3 days after puncture. Pain behavioral tests indicated that the anterior disc puncture did not induce pain behavior changes, whereas the posterior disc puncture resulted in mechanical allodynia from 1 day to 21 days after injury. Besides, cytokine expression was significantly increased in dorsal root ganglion (DRG) at 1 and 2 weeks after posterior puncture, but not after the anterior puncture. These findings indicate the NP of the degenerative disc expresses different levels of inflammatory cytokines, and posterior disc puncture produced mechanical allodynia. The expression phase of cytokines in the NP was accordance with mechanical hyperalgesia in the posterior disc puncture model. Both expression of cytokines and posterior annulus fibrosus (AF) rupture in degenerative intervertebral disc are essential for pain behavior changes.
authors.affiliation: Department of Spine Surgery, The First Affiliated Hospital of Sun Yat-sen University, No.58 Zhongshan 2nd Road, Guangzhou, 510080, China.
authors.forename: Zemin
authors.initials: Z
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Hao
authors.initials: H
authors.lastname: Yang
authors.forename: Jianru
authors.initials: J
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Kuibo
authors.initials: K
authors.lastname: Zhang
authors.forename: Wenbin
authors.initials: W
authors.lastname: Ding
authors.forename: Zhaomin
authors.initials: Z
authors.lastname: Zheng
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D016207:Cytokines / Q000096:biosynthesis*; D004195:Disease Models, Animal; D005727:Ganglia, Spinal / Q000378:metabolism; D006930:Hyperalgesia; D007403:Intervertebral Disc / Q000503:physiopathology*; D055959:Intervertebral Disc Degeneration / Q000503:physiopathology*; D017116:Low Back Pain / Q000503:physiopathology*; D008297:Male; D010146:Pain / Q000378:metabolism; D011677:Punctures; D051381:Rats; D017207:Rats, Sprague-Dawley; D012421:Rupture / Q000378:metabolism
chemical_list: D016207:Cytokines
keywords: animal model; annulus fibrosus rupture; cytokine; intervertebral disc degeneration; low back pain