title: Incorporation of essential oil in alginate microparticles by multiple emulsion/ionic gelation process.
abstract: In this study, an o/w/o multiple emulsion/ionic gelation method was developed for production of alginate microparticles loaded with Satureja hortensis essential oil (SEO). It was found that the essential oil concentration has significant influence on encapsulation efficiency (EE), loading capacity (LC) and size of microparticles. The values of EE, LC and particle mean diameter were about 52-66%, 20-26%, and 47-117 μm, respectively, when the initial SEO content was 1-3% (v/v) .The essential oil-loaded microparticles were porous, as displayed by scanning electron micrograph. The presence of SEO in alginate microparticles was confirmed by Fourier transform-infrared (FT-IR) spectroscopy and differential scanning calorimetry (DSC) analyses. SEO-loaded microparticles showed good antioxidant (with DPPH radical scavenging activity of 40.7-73.5%) and antibacterial properties; this effect was greatly improved when the concentration of SEO was 3% (v/v). S. aureus was found to be the most sensitive bacterium to SEO and showed a highest inhibition zone of 304.37 mm(2) in the microparticles incorporated with 3% (v/v) SEO. In vitro release studies showed an initial burst release and followed by a slow release. In addition, the release of SEO from the microparticles followed Fickian diffusion with acceptable release.
authors.affiliation: Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Seyede Marzieh
authors.initials: SM
authors.lastname: Hosseini
authors.forename: Hedayat
authors.initials: H
authors.lastname: Hosseini
authors.forename: Mohammad Amin
authors.initials: MA
authors.lastname: Mohammadifar
authors.forename: Amir Mohammad
authors.initials: AM
authors.lastname: Mortazavian
authors.forename: Abdorreza
authors.initials: A
authors.lastname: Mohammadi
authors.forename: Kianoosh
authors.initials: K
authors.lastname: Khosravi-Darani
authors.forename: Saeedeh
authors.initials: S
authors.lastname: Shojaee-Aliabadi
authors.forename: Solmaz
authors.initials: S
authors.lastname: Dehghan
authors.forename: Ramin
authors.initials: R
authors.lastname: Khaksar
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002152:Calorimetry, Differential Scanning; D004655:Emulsions / Q000737:chemistry* / Q000494:pharmacology; D005782:Gels / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry; D009822:Oils, Volatile / Q000737:chemistry*; D010316:Particle Size; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000464:Alginates; D000890:Anti-Infective Agents; D000975:Antioxidants; D004655:Emulsions; D005782:Gels; D006603:Hexuronic Acids; D052578:Ionic Liquids; D009822:Oils, Volatile; D020723:Glucuronic Acid
keywords: Alginate; Essential oil; Microparticle; Multiple emulsion; Satureja hortensis