title: Brief report: Dclk1 deletion in tuft cells results in impaired epithelial repair after radiation injury.
abstract: The role of Dclk1(+) tuft cells in the replacement of intestinal epithelia and reestablishing the epithelial barrier after severe genotoxic insult is completely unknown. Successful restoration requires precise coordination between the cells within each crypt subunit. While the mechanisms that control this response remain largely uncertain, the radiation model remains an exceptional surrogate for stem cell-associated crypt loss. Following the creation of Dclk1-intestinal-epithelial-deficient Villin-Cre;Dclk1(flox/flox) mice, widespread gene expression changes were detected in isolated intestinal epithelia during homeostasis. While the number of surviving crypts was unaffected, Villin-Cre;Dclk1(flox/flox) mice failed to maintain tight junctions and died at approximately 5 days, where Dclk1(flox/flox) mice lived until day 10 following radiation injury. These findings suggest that Dclk1 plays a functional role critical in the epithelial restorative response.
authors.affiliation: Department of Medicine, The University of Oklahoma Health Science Center, Oklahoma City, Oklahoma, USA; Department of Veterans Affairs Medical Center, Oklahoma City, Oklahoma, USA.
authors.forename: Randal
authors.initials: R
authors.lastname: May
authors.forename: Dongfeng
authors.initials: D
authors.lastname: Qu
authors.forename: Nathaniel
authors.initials: N
authors.lastname: Weygant
authors.forename: Parthasarathy
authors.initials: P
authors.lastname: Chandrakesan
authors.forename: Naushad
authors.initials: N
authors.lastname: Ali
authors.forename: Stanley A
authors.initials: SA
authors.lastname: Lightfoot
authors.forename: Linheng
authors.initials: L
authors.lastname: Li
authors.forename: Sripathi M
authors.initials: SM
authors.lastname: Sureban
authors.forename: Courtney W
authors.initials: CW
authors.lastname: Houchen
mesh_terms: D000818:Animals; D000089742:Doublecortin-Like Kinases; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology*; D017353:Gene Deletion; D005786:Gene Expression Regulation; D007413:Intestinal Mucosa / Q000473:pathology*; D051379:Mice; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011832:Radiation Injuries / Q000378:metabolism / Q000473:pathology*; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D016019:Survival Analysis; D014916:Whole-Body Irradiation; D014945:Wound Healing
chemical_list: D012333:RNA, Messenger; D051880:Receptors, Notch; D000089742:Doublecortin-Like Kinases; C485525:Dclk1 protein, mouse; D017346:Protein Serine-Threonine Kinases
keywords: Conditional knockout; Cre-loxP system; Irradiation; Notch; Stem cell-microenvironment interactions; Tissue regeneration; Tissue-specific stem cells