title: Cytotoxic effects of fucoidan nanoparticles against osteosarcoma.
abstract: In this study, we analyzed the size-dependent bioactivities of fucoidan by comparing the cytotoxic effects of native fucoidan and fucoidan lipid nanoparticles on osteosarcoma in vitro and in vivo. In vitro experiments indicated that nanoparticle fucoidan induced apoptosis of an osteosarcoma cell line more efficiently than native fucoidan. The more potent effects of nanoparticle fucoidan, relative to native fucoidan, were confirmed in vivo using a xenograft osteosarcoma model. Caco-2 cell transport studies showed that permeation of nanoparticle fucoidan was higher than native fucoidan. The higher bioactivity and superior bioavailability of nanoparticle fucoidan could potentially be utilized to develop novel therapies for osteosarcoma.
authors.affiliation: Department of Microbiology and Oncology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan. kimuraryuichiro@yahoo.co.jp.
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Kimura
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Rokkaku
authors.forename: Shinji
authors.initials: S
authors.lastname: Takeda
authors.forename: Masachika
authors.initials: M
authors.lastname: Senba
authors.forename: Naoki
authors.initials: N
authors.lastname: Mori
mesh_terms: D000818:Animals; D001682:Biological Availability; D018938:Caco-2 Cells; D063646:Carcinogenesis / Q000187:drug effects; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008168:Lung / Q000187:drug effects; D051379:Mice; D008809:Mice, Inbred C3H; D053758:Nanoparticles / Q000008:administration & dosage*; D012516:Osteosarcoma / Q000188:drug therapy*; D010539:Permeability; D011134:Polysaccharides / Q000494:pharmacology*
chemical_list: D011134:Polysaccharides; C007789:fucoidan