title: New culture medium concepts for cell transplantation.
abstract: BACKGROUND
  Before cell or tissue transplantation, cells or tissues have to be maintained for a certain period in vitro using culture medium and methods. Most culture media contain substances such as pH indicators and buffers. It is not known whether some of these substances are safe for subsequent application in the transplantation of cells or tissues into the human body. We investigated culture media and methods with respect to the safety of the components in future transplantation applications.
  
  
  METHODS
  A modified culture medium--medical fluid-based culture medium (FCM)--was designed by using various fluids and injectable drugs that are already currently permitted for use in clinical medicine. Medium components necessary for optimal cell growth were obtained from approved drugs. FCM was manufactured with adjusted final concentrations of the medium components similar to those in commercial Dulbecco's modified Eagle's medium (DMEM). In particular, 1029.40 mg/L amino acids, approximately 88.85 mg/L vitamins, 13,525.77 mg/L inorganic salts, and 4500 mg/L D-glucose comprise the high-glucose FCM. Next, human fat synovium-derived mesenchymal stem cells and rat H9c2 (2-1) cells were cultured under 2 conditions: (1) DMEM-high glucose (HG), an original commercial medium, and (2) optimized FCM-HG. We assessed the morphologies and proliferation rates of these cells.
  
  
  RESULTS
  We observed that FCM-HG was able to induce the growth of FS-MSC and commercially available H9c2 cell. The morphologies and proliferation patterns of these cells cultured under FCM-HG showed no differences compared with cells grown in DMEM-HG.
  
  
  CONCLUSIONS
  Our data suggest that FCM, which we developed for the first time according to the concept of drug repositioning, was a useful culture medium, especially in cultured cells intended for human cell transplantation.
authors.affiliation: Regenerative Medicine Laboratory, Center for Stem Cell Research, Department of Biomedical Science and Technology, Institute of Biomedical Science & Technology (IBST), Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul, Republic of Korea.
authors.forename: S
authors.initials: S
authors.lastname: Lee
authors.forename: B Y
authors.initials: BY
authors.lastname: Kim
authors.forename: J E
authors.initials: JE
authors.lastname: Yeo
authors.forename: J G
authors.initials: JG
authors.lastname: Nemeno
authors.forename: Y H
authors.initials: YH
authors.lastname: Jo
authors.forename: W
authors.initials: W
authors.lastname: Yang
authors.forename: B M
authors.initials: BM
authors.lastname: Nam
authors.forename: S
authors.initials: S
authors.lastname: Namoto
authors.forename: S
authors.initials: S
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: K M
authors.initials: KM
authors.lastname: Lee
authors.forename: H S
authors.initials: HS
authors.lastname: Hwang
authors.forename: J I
authors.initials: JI
authors.lastname: Lee
mesh_terms: D017690:Cell Transplantation; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans
chemical_list: D003470:Culture Media