title: Autoantibody detection to tumor-associated antigens of P53, IMP1, P16, cyclin B1, P62, C-myc, Survivn, and Koc for the screening of high-risk subjects and early detection of esophageal squamous cell carcinoma.
abstract: The aim of this study was to evaluate the diagnostic values by detecting sera autoantibodies to eight tumor-associated antigens (TAAs) of P53, IMP1, P16, cyclin B1, P62, C-myc, Survivn and Koc full-length recombinant proteins for the screening of high-risk subjects and early detection of esophageal squamous cell carcinoma (ESCC). Enzyme-linked immunosorbent assay was used to detect autoantibodies against the eight selected TAAs in 567 sera samples from four groups, including 200 individuals with normal esophageal epithelia (NOR), 214 patients with esophageal basal cell hyperplasia (BCH), 65 patients with esophageal dysplasia (DYS), and 88 patients with ESCC. In addition, the expression of the eight antigens in esophageal tissues was analyzed by immunohistochemistry. Statistically significant distribution differences were identified among the four groups for each of the individual autoantibodies to six TAAs (P53, IMP1, P16, cyclin B1, P62, and C-myc); the detection rates of antoantibodies were positively correlated with the progression of ESCC. When autoantibody assay successively accumulated to six TAAs (P53, IMP1, P16, cyclin B1, P62, and C-myc), a stepwise increased detection frequency of autoantibodies was found in the four sera groups (6% in NOR, 18% in BCH, 38% in DYS, and 64% in ESCC, respectively), the risks to BHC, DYS, and ESCC steadily increased about 3-, 9-, and 27-folds. The sensitivity and the specificity for autoantibodies against the six TAAs in diagnosing ESCC reached up to 64% and 94%, respectively. The area under the receiver operating characteristic curve for the six anti-TAA autoantibodies was 0.78 (95% confidence interval 0.74-0.83). No more increasing in sensitivity was found with the addition of new anti-TAA autoantibodies. A combination detection of autoantibodies to TAAs might distinguish ESCC patients from normal individuals and the patients with esophageal precancerous lesions.
authors.affiliation: Henan Key Laboratory for Esophageal Cancer Research, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: S L
authors.initials: SL
authors.lastname: Zhou
authors.forename: W B
authors.initials: WB
authors.lastname: Yue
authors.forename: Z M
authors.initials: ZM
authors.lastname: Fan
authors.forename: F
authors.initials: F
authors.lastname: Du
authors.forename: B C
authors.initials: BC
authors.lastname: Liu
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.forename: X N
authors.initials: XN
authors.lastname: Han
authors.forename: J W
authors.initials: JW
authors.lastname: Ku
authors.forename: X K
authors.initials: XK
authors.lastname: Zhao
authors.forename: P
authors.initials: P
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Cui
authors.forename: F Y
authors.initials: FY
authors.lastname: Zhou
authors.forename: L Q
authors.initials: LQ
authors.lastname: Zhang
authors.forename: X P
authors.initials: XP
authors.lastname: Fan
authors.forename: Y F
authors.initials: YF
authors.lastname: Zhou
authors.forename: L L
authors.initials: LL
authors.lastname: Zhu
authors.forename: H Y
authors.initials: HY
authors.lastname: Liu
authors.forename: L D
authors.initials: LD
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000276:immunology* / Q000378:metabolism; D001323:Autoantibodies / Q000097:blood*; D014408:Biomarkers, Tumor / Q000276:immunology / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000276:immunology / Q000378:metabolism; D056744:Cyclin B1 / Q000276:immunology / Q000378:metabolism; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004268:DNA-Binding Proteins / Q000276:immunology / Q000378:metabolism; D042241:Early Diagnosis; D004938:Esophageal Neoplasms / Q000175:diagnosis* / Q000276:immunology / Q000378:metabolism; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000276:immunology / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000276:immunology / Q000378:metabolism; D000077022:Survivin; D014157:Transcription Factors / Q000276:immunology / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000276:immunology / Q000378:metabolism
chemical_list: D000951:Antigens, Neoplasm; D001323:Autoantibodies; C107489:BIRC5 protein, human; D014408:Biomarkers, Tumor; C000614131:CDKN2A protein, human; D056744:Cyclin B1; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004268:DNA-Binding Proteins; C406144:IGF2BP1 protein, human; C498780:IGF2BP3 protein, human; D051034:Inhibitor of Apoptosis Proteins; C116115:MYCBP protein, human; D009363:Neoplasm Proteins; C118585:P62 protein, human; D016601:RNA-Binding Proteins; D000077022:Survivin; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53
keywords: autoantibody; esophageal squamous cell carcinoma; precancerous lesion; tumor-associated antigen (TAA)