title: Influence of salinity and temperature on the activity of biosurfactants by polychaete-associated isolates.
abstract: Influence of different parameters on biosurfactant (BS) activity was carried out on strains that were isolated from the polychaetes Megalomma claparedei, Sabella spallanzanii and Branchiomma luctuosum and additional 30 strains that were previously identified as potential BS producers from crude oil enrichments of the same polychaete specimens. The selection of BS-producing strains from polychaete natural samples was carried out by using standard screening tests. The BS activity by each isolate was evaluated for the effect of salinity and temperature on emulsion production and surface tension reduction, during incubation in mineral medium supplemented with tetradecane or diesel oil. All isolates showed a similar time course of BS activity, and the latter was more influenced by salinity rather than temperature. Some of the BS producers belonged to genera that have not (i.e. Citricoccus, Cellulophaga, Tenacibaculum and Maribacter) or have poorly been (Psychrobacter, Vibrio, and Pseudoalteromonas) reported as able to produce BSs. This is remarkable as some of them have previously been detected in hydrocarbon-enriched samples. Results confirm that filter-feeding polychaetes are an efficient source for the isolation of BS producers.
authors.affiliation: Department of Biological and Environmental Sciences, University of Messina, Viale Ferdinando Stagno d'Alcontrès 31, 98166, Messina, Italy.
authors.forename: Carmen
authors.initials: C
authors.lastname: Rizzo
authors.forename: Luigi
authors.initials: L
authors.lastname: Michaud
authors.forename: Christoph
authors.initials: C
authors.lastname: Syldatk
authors.forename: Rudolf
authors.initials: R
authors.lastname: Hausmann
authors.forename: Emilio
authors.initials: E
authors.lastname: De Domenico
authors.forename: Angelina
authors.initials: A
authors.lastname: Lo Giudice
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D011077:Polychaeta / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017423:Sequence Analysis, RNA; D013500:Surface Tension; D013501:Surface-Active Agents / Q000378:metabolism*; D013696:Temperature
chemical_list: D012336:RNA, Ribosomal, 16S; D013501:Surface-Active Agents