title: Antiherpetic Effects of Gynura procumbens.
abstract: The ethanol extract of Gynura procumbens showed virucidal and antireplicative actions against herpes simplex virus HSV-1 and HSV-2. It was further chromatographed on MCI gel CHP20P column giving the extract fractions F1 (water), F2 (water-methanol) F3 (methanol), and F4 (ethyl acetate). All but F1 had virucidal action against both viral types. We reported here the active compounds from F2 and F3. The antiherpetic compounds of F2 was a mixture of dicaffeoylquinic acids with virucidal and antireplicative actions against HSV-2 (IC50 96.0 and 61.0  μ g/mL, resp.) Virucidal compounds of F3 were a mixture of β -sitosterol and stigmasterol (IC50 250.0  μ g/mL against HSV-1), a mixture of β -sitosteryl and stigmasteryl glucosides (IC50 50.0  μ g/mL against HSV-2) and 1, 2-bis-dodecanoyl-3- α -D-glucopyranosyl-sn-glycerol (IC50 of 40.0  μ g/mL against HSV-2). Herbal products containing 1 and 2% of standardized ethanol extract were prepared. Double-blind randomized controlled clinical trial of the products was performed in patients with recurrent herpes labialis. Results showed that the number of patients, whose lesions healed within 7 days and the average healing time of both groups differed insignificantly. Viral culture on D7 indicated a decrease of infected patients from 48.7% to 7.69% in treated group whereas in placebo group the infected patients decreased from 31.25% to 20.00%. The viral reduction in treated group indicated the benefit of the product. Insignificant result might arise from a low number of participated patients and insufficient concentration of plant extract in herbal product.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, 447 Sri Ayudhya Road, Rajathevi District, Bangkok 10400, Thailand ; Pharmaceutical and Natural Products Department, Thailand Institute of Scientific and Technological Research, Pathum Thani 12120, Thailand.
authors.forename: Siripen
authors.initials: S
authors.lastname: Jarikasem
authors.forename: Somyot
authors.initials: S
authors.lastname: Charuwichitratana
authors.forename: Sontana
authors.initials: S
authors.lastname: Siritantikorn
authors.forename: Wasan
authors.initials: W
authors.lastname: Chantratita
authors.forename: Magdy
authors.initials: M
authors.lastname: Iskander
authors.forename: August Wilhelm
authors.initials: AW
authors.lastname: Frahm
authors.forename: Weena
authors.initials: W
authors.lastname: Jiratchariyakul