title: Nanopore-based identification of individual nucleotides for direct RNA sequencing.
abstract: We describe a label-free ribobase identification method, which uses ionic current measurement to resolve ribonucleoside monophosphates or diphosphates in α-hemolysin protein nanopores containing amino-cyclodextrin adapters. The accuracy of base identification is further investigated through the use of a guanidino-modified adapter. On the basis of these findings, an exosequencing approach is envisioned in which a processive exoribonuclease (polynucleotide phosphorylase) presents sequentially cleaved ribonucleoside diphosphates to a nanopore.
authors.affiliation: Department of Chemistry, University of Oxford , Oxford OX1 3TA, United Kingdom.
authors.forename: Mariam
authors.initials: M
authors.lastname: Ayub
authors.forename: Steven W
authors.initials: SW
authors.lastname: Hardwick
authors.forename: Ben F
authors.initials: BF
authors.lastname: Luisi
authors.forename: Hagan
authors.initials: H
authors.lastname: Bayley
mesh_terms: D001483:Base Sequence; D006460:Hemolysin Proteins / Q000737:chemistry; D058608:Nanopores; D049329:Nanostructures / Q000737:chemistry; D009711:Nucleotides / Q000737:chemistry / Q000302:isolation & purification*; D017423:Sequence Analysis, RNA
chemical_list: D006460:Hemolysin Proteins; D009711:Nucleotides