title: Expression of the cloned endo-1,3-1,4-β-glucanase gene of Bacillus subtilis in Saccharomyces cerevisiae.
abstract: A cloned endo-1,3-1,4-β-glucanase gene from the Gram-positive bacterium B. subtilis has been located by deletion analysis on a 1.4 kb PvuI-ClaI DNA fragment. This gene has been sub-cloned in the yeast LEU2 vector pJDB207 to produce a hybrid plasmid designated pEHB9. pEHB9 has been transformed to S. cerevisiae and shown to direct the synthesis of an endo-1,3-1,4-β-glucanase in yeast. The β-glucanase activity was low and could only be detected in crude cell extracts of yeast harbouring pEHB9.
authors.affiliation: Research Department; Bass PLC, 137 High Street, DE14 1JZ, Burton on Trent, Great Britain.
authors.forename: E
authors.initials: E
authors.lastname: Hinchliffe
authors.forename: W G
authors.initials: WG
authors.lastname: Box