title: Jasmonic acid and glucose synergistically modulate the accumulation of glucosinolates in Arabidopsis thaliana.
abstract: The interplay of plant hormones and glucose (Glu) in regulating glucosinolate accumulation in Arabidopsis thaliana was investigated in this study. Glucose-induced glucosinolate biosynthesis was enhanced significantly by the addition of jasmonic acid (JA), whereas the synergistic effect of salicylic acid (SA) and Glu was less obvious. The enhanced glucosinolate accumulation is associated with elevated expression of genes in glucosinolate biosynthetic pathway, as well as the transcription factors involved in their regulation, such as MYB28, MYB29, MYB34, and MYB122. The induction of indolic and aliphatic glucosinolates after treatment with JA and Glu in JA-insensitive mutants, coi1, jar1, and jin1, was compromised. Moreover, the effect of JA and Glu on glucosinolate contents was dramatically reduced in Glu-insensitive mutants, rgs1-2 and abi5-7. These results indicate a crosstalk between JA and Glu signalling in the regulation of glucosinolate biosynthesis. JA signalling, RGS1 (the putative membrane receptor of Glu signalling), and ABI5, are involved in the synergistic effect of JA and Glu on glucosinolate accumulation.
authors.affiliation: Department of Horticulture, Zhejiang University, Hangzhou 310058, China.
authors.forename: Rongfang
authors.initials: R
authors.lastname: Guo
authors.forename: Wangshu
authors.initials: W
authors.lastname: Shen
authors.forename: Hongmei
authors.initials: H
authors.lastname: Qian
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Lihong
authors.initials: L
authors.lastname: Liu
authors.forename: Qiaomei
authors.initials: Q
authors.lastname: Wang
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism* / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D005961:Glucosinolates / Q000378:metabolism*; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D054883:Oxylipins / Q000378:metabolism* / Q000494:pharmacology; D020710:RGS Proteins / Q000235:genetics / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000099224:p300-CBP-Associated Factor
chemical_list: D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D003517:Cyclopentanes; D005947:Glucose; D005961:Glucosinolates; D051548:Histone Acetyltransferases; D054883:Oxylipins; D020710:RGS Proteins; D020156:Salicylic Acid; D014157:Transcription Factors; C409743:ABI5 protein, Arabidopsis; C523552:Myb29 protein, Arabidopsis; C477755:RGS1 protein, Arabidopsis; C011006:jasmonic acid; C474636:GCN5 protein, Arabidopsis; D000099224:p300-CBP-Associated Factor
keywords: ABI5; Arabidopsis thaliana; glucose; glucosinolate; jasmonic acid; salicylic acid.