title: Impact of TRPV3 on the development of allergic dermatitis as a dendritic cell modulator.
abstract: The transient receptor potential channel vanilloid subfamily V member 3 (TRPV3), which functions as a thermosensor in keratinocytes, plays an important role in the development of allergic and itchy dermatitis in rodents. Although real-time PCR analysis using lesional and non-lesional skin samples from patients with atopic dermatitis showed that TRPV3 was expressed in lesional skin, the role that TRPV3 plays in patients with dermatitis is still relatively obscure. Here, we determined whether TRPV3 was a dendritic cell (DC) modulator using DS-Nh mice with a gain-of-function mutation in TRPV3 (TRPV3Gly573Ser), because increasing skin temperature is associated with the modulation of dermal dendritic cells (DCs). Interestingly, increased responses to haptens by skin and DCs were observed in DS-Nh mice compared with those from DS mice with wild-type TRPV3. Increased thymic stromal lymphopoietin (TSLP) responses were also observed in keratinocytes from DS-Nh mice compared with those from DS mice. Taken together, we propose that the DS-Nh mouse is a good model to use in order to better understand the role of this orphan channel and that TRPV3 may represent a new therapeutic target in certain types of dermatitis through the control of DCs.
authors.affiliation: Department of Biology, Graduate School of Science, Kobe University, Kobe, Japan; Shionogi Medicinal Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.
authors.forename: Erika
authors.initials: E
authors.lastname: Yamamoto-Kasai
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Yasui
authors.forename: Michitaka
authors.initials: M
authors.lastname: Shichijo
authors.forename: Tsuneaki
authors.initials: T
authors.lastname: Sakata
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yoshioka
mesh_terms: D000818:Animals; D002465:Cell Movement; D002469:Cell Separation; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D003872:Dermatitis / Q000378:metabolism*; D003877:Dermatitis, Contact / Q000378:metabolism; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D006241:Haptens / Q000737:chemistry; D006967:Hypersensitivity / Q000378:metabolism; D007249:Inflammation; D015603:Keratinocytes / Q000166:cytology; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018345:Mice, Knockout; D010641:Phenotype; D012881:Skin Temperature; D050916:TRPV Cation Channels / Q000502:physiology*; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D016207:Cytokines; D006241:Haptens; D050916:TRPV Cation Channels; C486760:Trpv3 protein, mouse; D016650:Fluorescein-5-isothiocyanate; D000094632:Thymic Stromal Lymphopoietin; C000721967:TSLP protein, mouse
keywords: atopic dermatitis; contact hypersensitivity; dendritic cell; thymic stromal lymphopoietin; transient receptor potential vanilloid subfamily V member 3