title: Neuronal cell differentiation of mesenchymal stem cells originating from canine amniotic fluid.
abstract: The amniotic fluid contains mesenchymal stem cells (MSCs) and can be readily available for tissue engineering. Regenerative treatments such as tissue engineering, cell therapy, and transplantation show potential in clinical trials of degenerative diseases. Disease presentation and clinical responses in the Canis familiaris not only are physiologically similar to human compared with other traditional mammalian models but is also a suitable model for human diseases. The aim of this study was to investigate whether canine amniotic-fluid-derived mesenchymal stem cells (cAF-MSCs) can differentiate into neural precursor cells in vitro when exposed to neural induction reagent. During neural differentiation, cAF-MSCs progressively acquire neuron-like morphology. Messenger RNA (mRNA) expression levels of neural-specific genes, such as NEFL, NSE, and TUBB3 (βIII-tubulin) dramatically increased in the differentiated cAF-MSCs after induction. In addition, protein expression levels of nestin, βIII-tubulin, and tyrosine hydroxylase remarkably increased in differentiated cAF-MSCs. This study demonstrates that cAF-MSCs have great potential for neural precursor differentiation in vitro. Therefore, amniotic fluid may be a suitable alternative source of stem cells and can be applied to cell therapy in neurodegenerative diseases.
authors.affiliation: Laboratory of Animal Reproduction and Physiology, Department of Animal Science and Biotechnology, College of Agriculture Life Science, Chungnam National University, Daejeon, 305-764, Republic of Korea.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Kim
authors.forename: Kyung-Bon
authors.initials: KB
authors.lastname: Lee
authors.forename: Jung
authors.initials: J
authors.lastname: Yu
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Lee
authors.forename: Keun Jung
authors.initials: KJ
authors.lastname: Kim
authors.forename: Kil-Woo
authors.initials: KW
authors.lastname: Han
authors.forename: Kang-Sun
authors.initials: KS
authors.lastname: Park
authors.forename: Dong-Soo
authors.initials: DS
authors.lastname: Lee
authors.forename: Min Kyu
authors.initials: MK
authors.lastname: Kim
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D064987:Cell- and Tissue-Based Therapy; D002478:Cells, Cultured; D004285:Dogs; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D015870:Gene Expression; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D064231:Nestin / Q000378:metabolism; D019636:Neurodegenerative Diseases / Q000628:therapy; D016900:Neurofilament Proteins / Q000378:metabolism; D009474:Neurons; D012333:RNA, Messenger / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D064231:Nestin; D016900:Neurofilament Proteins; D012333:RNA, Messenger; C498585:TUBB3 protein, human; D014404:Tubulin; C044882:neurofilament protein L; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D014446:Tyrosine 3-Monooxygenase