title: Implantation of tetrapod-shaped granular artificial bones or β-tricalcium phosphate granules in a canine large bone-defect model.
abstract: We investigated biodegradability and new bone formation after implantation of tetrapod-shaped granular artificial bone (Tetrabone®) or β-tricalcium phosphate granules (β-TCP) in experimental critical-size defects in dogs, which were created through medial and lateral femoral condyles. The defect was packed with Tetrabone® (Tetrabone group) or β-TCP (β-TCP group) or received no implant (control group). Computed tomography (CT) was performed at 0, 4 and 8 weeks after implantation. Micro-CT and histological analysis were conducted to measure the non-osseous tissue rate and the area and distribution of new bone tissue in the defect at 8 weeks after implantation. On CT, β-TCP was gradually resorbed, while Tetrabone® showed minimal resorption at 8 weeks after implantation. On micro-CT, non-osseous tissue rate of the control group was significantly higher compared with the β-TCP and Tetrabone groups (P<0.01), and that of the β-TCP group was significantly higher compared with the Tetrabone group (P<0.05). On histology, area of new bone tissue of the β-TCP group was significantly greater than those of the Tetrabone and control groups (P<0.05), and new bone distribution of the Tetrabone group was significantly greater than those of the β-TCP and control groups (P<0.05). These results indicate differences in biodegradability and connectivity of intergranule pore structure between study samples. In conclusion, Tetrabone® may be superior for the repair of large bone defects in dogs.
authors.affiliation: Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Sungjin
authors.initials: S
authors.lastname: Choi
authors.forename: I-Li
authors.initials: IL
authors.lastname: Liu
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Muneki
authors.initials: M
authors.lastname: Honnami
authors.forename: Takamasa
authors.initials: T
authors.lastname: Sakai
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Ohba
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Echigo
authors.forename: Shigeki
authors.initials: S
authors.lastname: Suzuki
authors.forename: Ryouhei
authors.initials: R
authors.lastname: Nishimura
authors.forename: Ung-Il
authors.initials: UI
authors.lastname: Chung
authors.forename: Nobuo
authors.initials: N
authors.lastname: Sasaki
authors.forename: Manabu
authors.initials: M
authors.lastname: Mochizuki
mesh_terms: D020341:Absorbable Implants; D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology*; D001842:Bone and Bones / Q000002:abnormalities*; D002130:Calcium Phosphates / Q000494:pharmacology*; D004285:Dogs; D006652:Histological Techniques; D055114:X-Ray Microtomography
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate