title: AKAPS act in a two-step mechanism of memory acquisition.
abstract: Defining the molecular and neuronal basis of associative memories is based upon behavioral preparations that yield high performance due to selection of salient stimuli, strong reinforcement, and repeated conditioning trials. One of those preparations is the Drosophila aversive olfactory conditioning procedure where animals initiate multiple memory components after experience of a single cycle training procedure. Here, we explored the analysis of acquisition dynamics as a means to define memory components and revealed strong correlations between particular chronologies of shock impact and number experienced during the associative training situation and subsequent performance of conditioned avoidance. Analyzing acquisition dynamics in Drosophila memory mutants revealed that rutabaga (rut)-dependent cAMP signals couple in a divergent fashion for support of different memory components. In case of anesthesia-sensitive memory (ASM) we identified a characteristic two-step mechanism that links rut-AC1 to A-kinase anchoring proteins (AKAP)-sequestered protein kinase A at the level of Kenyon cells, a recognized center of olfactory learning within the fly brain. We propose that integration of rut-derived cAMP signals at level of AKAPs might serve as counting register that accounts for the two-step mechanism of ASM acquisition.
authors.affiliation: Free University Berlin, Biology-Genetics, D-14195 Berlin, Germany and Max-Delbrück-Centrum für Molekulare Medizin, 13125 Berlin, Germany.
authors.forename: Lisa
authors.initials: L
authors.lastname: Scheunemann
authors.forename: Philipp
authors.initials: P
authors.lastname: Skroblin
authors.forename: Christian
authors.initials: C
authors.lastname: Hundsrucker
authors.forename: Enno
authors.initials: E
authors.lastname: Klussmann
authors.forename: Marina
authors.initials: M
authors.lastname: Efetova
authors.forename: Martin
authors.initials: M
authors.lastname: Schwärzel
mesh_terms: D054758:A Kinase Anchor Proteins / Q000502:physiology*; D000262:Adenylyl Cyclases / Q000502:physiology*; D000818:Animals; D000242:Cyclic AMP / Q000502:physiology; D004330:Drosophila; D029721:Drosophila Proteins / Q000502:physiology*; D005260:Female; D008297:Male; D008568:Memory / Q000502:physiology*; D012054:Reinforcement, Psychology; D012903:Smell / Q000502:physiology*
chemical_list: D054758:A Kinase Anchor Proteins; D029721:Drosophila Proteins; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; C479926:Rut protein, Drosophila