title: Population genetics and forensic efficiency of twenty-one novel microsatellite loci of Chinese Yi ethnic group.
abstract: In this study, we investigated polymorphic distributions of allelic frequencies and forensic genetic parameters of 21 novel autosomal microsatellite loci from 110 unrelated healthy individuals of Chinese Yi ethnic group. Expected heterozygosity, power of discrimination, and polymorphic information content ranged from 0.617 to 0.812, 0.777 to 0.936 and 0.560 to 0.790. The microsatellite loci showed high forensic efficiency. The total discrimination power and cumulate probability of exclusion were 0.99999999999999999986902 and 0.999998818, respectively. Locus-by-locus allelic frequencies were compared using analysis of molecular variance (AMOVA) method, and the statistically significant differences were observed between Yi group and Russian, Tujia, Kazak, Bai, Ningxia Han, Salar, Tibetan, and Uigur groups at 5, 6, 7, 7, 7, 8, 12, and 13 loci, respectively. The results of genetic distance comparisons, genetic structure analyses, and principal component analysis all indicated that the Yi group showed relatively short genetic relationships with Russian, Salar, and Bai group. The experimental results showed that the 21 loci in the multiplex system provided highly polymorphic information and forensic efficiency for forensic individual identification and paternity testing, also basic population data for population genetics and anthropological research.
authors.affiliation: School of Medicine, Xi'an Jiaotong University, Xi'an, P. R. China.
authors.forename: Bo-Feng
authors.initials: BF
authors.lastname: Zhu
authors.forename: Feng
authors.initials: F
authors.lastname: Pan
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Shen
authors.forename: Jiang-Wei
authors.initials: JW
authors.lastname: Yan
authors.forename: Hong-Dan
authors.initials: HD
authors.lastname: Wang
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Guo
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Wang
authors.forename: Hao-Tian
authors.initials: HT
authors.lastname: Meng
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Liu
authors.forename: Yu-Dang
authors.initials: YD
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Hang
authors.initials: H
authors.lastname: Jing
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
mesh_terms: D044466:Asian People / Q000235:genetics*; D002681:China; D005006:Ethnicity / Q000235:genetics*; D005787:Gene Frequency; D005828:Genetics, Population / Q000379:methods*; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D025341:Principal Component Analysis
keywords: AMOVA; Forensic efficiency; Genetic distance; Microsatellite; Principal component analysis