title: Mushroom hunting by using bioinformatics: application of a predictive framework facilitates the selective identification of sesquiterpene synthases in basidiomycota.
abstract: The Basidiomycota fungi represent a diverse source of natural products, particularly the sesquiterpenoids. Recently, genome sequencing, genome mining, and the subsequent discovery of a suite of sesquiterpene synthases in Omphalotus olearius was described. A predictive framework was developed to facilitate the discovery of sesquiterpene synthases in Basidiomycota. Phylogenetic analyses indicated a conservation of both sequence and initial cyclization mechanisms used. Here, the first robust application of this predictive framework is reported. It was used to selectively identify sesquiterpene synthases that follow 1,6-, 1,10-, and 1,11-cyclization mechanisms in the crust fungus Stereum hirsutum. The successful identification and characterization of a 1,6- and a 1,10-cyclizing sesquiterpene synthase, as well as three 1,11-cyclizing Δ(6) -protoilludene synthases, is described. This study verifies the accuracy and utility of the predictive framework as a roadmap for the discovery of specific sesquiterpene synthases from Basidiomycota, and thus represents an important step forward in natural product discovery.
authors.affiliation: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, 1479 Gortner Avenue, St. Paul, MN 55108 (USA).
authors.forename: Maureen B
authors.initials: MB
authors.lastname: Quin
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Flynn
authors.forename: Grayson T
authors.initials: GT
authors.lastname: Wawrzyn
authors.forename: Swati
authors.initials: S
authors.lastname: Choudhary
authors.forename: Claudia
authors.initials: C
authors.lastname: Schmidt-Dannert
mesh_terms: D001487:Basidiomycota / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D001688:Biological Products / Q000737:chemistry / Q000378:metabolism*; D003001:Cloning, Molecular; D019295:Computational Biology / Q000379:methods*; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D010802:Phylogeny; D012717:Sesquiterpenes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001688:Biological Products; D012717:Sesquiterpenes; D008025:Ligases
keywords: GC/MS; anticancer agents; biosynthesis; kinetics; natural products