title: Simultaneous determination of 11 monohydroxylated PAHs in human urine by stir bar sorptive extraction and liquid chromatography/tandem mass spectrometry.
abstract: A simple and sensitive method for simultaneous determination of 11 monohydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) in human urines has been developed based on stir bar sorptive extraction (SBSE) and liquid chromatography/tandem mass spectrometry (HPLC-MS/MS). Factors that may influence the extraction efficiency, such as pH value of matrix, extraction time, desorption solvents and desorption time were optimized. Validation results showed that the method has high sensitivity (quantification limits of 1-3 pg/mL), good reproducibility (RSD between 3.1 and 13.0%) and spiked recoveries (71.9-133.2%). The proposed method was also applied to analysis urines of smokers and nonsmokers, ten trace OH-PAHs were determined and compared between two groups. For the ease operation and satisfactory validiation results, SBSE coupled to HPLC-MS/MS may be an excellent alternative method for trace analysis of OH-PAHs in human urines.
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Zhao
authors.forename: Yusong
authors.initials: Y
authors.lastname: Chen
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.forename: Jingjing
authors.initials: J
authors.lastname: Yu
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.forename: Fuwei
authors.initials: F
authors.lastname: Xie
authors.forename: Huimin
authors.initials: H
authors.lastname: Liu
authors.forename: Jianping
authors.initials: J
authors.lastname: Xie
mesh_terms: D016022:Case-Control Studies; D002853:Chromatography, Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006900:Hydroxylation; D057230:Limit of Detection; D011084:Polycyclic Aromatic Hydrocarbons / Q000652:urine*; D015203:Reproducibility of Results; D012907:Smoking / Q000652:urine*; D052617:Solid Phase Microextraction / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons
keywords: HPLC–MS/MS; Monohydroxylated PAHs; Stir bar sorptive extraction