title: Two-dimensional gel-based proteomic of the caries causative bacterium Streptococcus mutans UA159 and insight into the inhibitory effect of carolacton.
abstract: Streptococcus mutans is considered to be the most cariogenic organism. Carolacton, isolated from the myxobacterium Sorangium cellulosum, shows the ability to disturb S. mutans biofilm viability that makes it a potential anti-biofilm drug. However, the molecular mechanism of carolacton remains to be elucidated. In order to use proteomics to characterize the effect of carolacton, we constructed a 2DE-based proteome reference map of the cytoplasmic and extracellular proteins for S. mutans in the present study. In total, 239 protein spots representing 192 different cytoplasmic proteins were identified by MALDI-TOF MS and PMF. This represents the highest number of identified proteins so far for S. mutans UA159 in the pI range of 4-7 and would benefit further research on the physiology and pathogenicity of this strain. Based on the constructed reference map, the inhibitory effects of carolacton on S. mutans biofilm and planktonic-growing cells were investigated. The results of the comparative proteome analysis indicate that carolacton exerts its inhibitory effects by disturbing the peptidoglycan biosynthesis and degradation and thereby causes damages to the integrity of the cell envelope, leading ultimately to cell death.
authors.affiliation: Institute for Bioprocess and Biosystems Engineering, Hamburg University of Technology (IBB/TUHH), Hamburg, Germany; Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Jinshan
authors.initials: J
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: An-Ping
authors.initials: AP
authors.lastname: Zeng
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism*; D018441:Biofilms / Q000187:drug effects*; D003731:Dental Caries / Q000382:microbiology; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D018942:Macrolides / Q000494:pharmacology*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D013295:Streptococcus mutans / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018942:Macrolides; D020543:Proteome; C553423:carolacton
keywords: Biofilm inhibitor; Carolacton; Cytoplasmic proteome; Microbiology; Streptococcus mutans UA159