title: Geniposide regulates glucose-stimulated insulin secretion possibly through controlling glucose metabolism in INS-1 cells.
abstract: Glucose-stimulated insulin secretion (GSIS) is essential to the control of metabolic fuel homeostasis. The impairment of GSIS is a key element of β-cell failure and one of causes of type 2 diabetes mellitus (T2DM). Although the KATP channel-dependent mechanism of GSIS has been broadly accepted for several decades, it does not fully describe the effects of glucose on insulin secretion. Emerging evidence has suggested that other mechanisms are involved. The present study demonstrated that geniposide enhanced GSIS in response to the stimulation of low or moderately high concentrations of glucose, and promoted glucose uptake and intracellular ATP levels in INS-1 cells. However, in the presence of a high concentration of glucose, geniposide exerted a contrary role on both GSIS and glucose uptake and metabolism. Furthermore, geniposide improved the impairment of GSIS in INS-1 cells challenged with a high concentration of glucose. Further experiments showed that geniposide modulated pyruvate carboxylase expression and the production of intermediates of glucose metabolism. The data collectively suggest that geniposide has potential to prevent or improve the impairment of insulin secretion in β-cells challenged with high concentrations of glucose, likely through pyruvate carboxylase mediated glucose metabolism in β-cells.
authors.affiliation: Chongqing Key Laboratory of Catalysis & Functional Organic Molecules, Chongqing Technology and Business University, Chongqing, China ; Chongqing Key Laboratory of Natural Medicine Research, Chongqing Technology and Business University, Chongqing, China ; Aquatic and Crop Resource Development, National Research Council of Canada, Charlottetown, Prince Edward Island, Canada.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Liu
authors.forename: Lixia
authors.initials: L
authors.lastname: Guo
authors.forename: Fei
authors.initials: F
authors.lastname: Yin
authors.forename: Yonglan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zixuan
authors.initials: Z
authors.lastname: Liu
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D002460:Cell Line; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D039823:Iridoids / Q000494:pharmacology*; D051381:Rats
chemical_list: D007328:Insulin; D039823:Iridoids; C007835:geniposide; D005947:Glucose