title: Monitoring of patients supported by extracorporeal membrane oxygenation for systemic infections by broad-range rRNA gene PCR amplification and sequence analysis.
abstract: The rRNA gene PCR and sequencing test, SepsiTest, was compared with blood culture (BC) regarding the diagnosis of pathogens in 160 blood samples drawn from 28 patients during extracorporeal membrane oxygenation. With 45% of positive samples, SepsiTest was 13 to 75 h faster than BC. SepsiTest indicated bacteremias in 25% of patients who were BC negative.
authors.affiliation: Department of Thoracic, Transplant, and Cardiovascular Surgery, Hannover Medical School, Hannover, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Orszag
authors.forename: Claudia
authors.initials: C
authors.lastname: Disqué
authors.forename: Sonja
authors.initials: S
authors.lastname: Keim
authors.forename: Michael G
authors.initials: MG
authors.lastname: Lorenz
authors.forename: Olaf
authors.initials: O
authors.lastname: Wiesner
authors.forename: Johannes
authors.initials: J
authors.lastname: Hadem
authors.forename: Meike
authors.initials: M
authors.lastname: Stiesch
authors.forename: Axel
authors.initials: A
authors.lastname: Haverich
authors.forename: Christian
authors.initials: C
authors.lastname: Kühn
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015199:Extracorporeal Membrane Oxygenation / Q000009:adverse effects*; D005260:Female; D020459:Genes, rRNA / Q000235:genetics*; D006801:Humans; D008297:Male; D008828:Microbiological Techniques / Q000379:methods*; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D018805:Sepsis / Q000175:diagnosis*; D017421:Sequence Analysis / Q000379:methods; D013997:Time Factors; D055815:Young Adult