title: The antioxidant properties of new dimer and two monomers of phenolic acid amides isolated from Limoniastrum guyonianum.
abstract: Limoniastramide, a new dimer of phenolic acid amide, isolated from Limoniastrum guyonianum, along with two natural monomers N-E-caffeoyl tyramine (1) and N-E-feruloyl tyramine (2), using centrifugal partition chromatography (CPC). Their structures were determined on the basis of spectroscopic data analysis. We investigate the antioxidant activities of Limoniastrum amides using various in vitro assays. Results showed that N-E-feruloyl tyramine and N-E-caffeoyl tyramine exhibited the highest DPPH scavenging activity compared to the dimer (IC50=0.5, 0.6 and 6.5μg/ml, respectively). In addition, they have significant capacities to inhibit the bleaching of β-carotene. Limoniastramide presented the best activity with an IC50 value equal to 8μg/ml. Finally, the N-E-caffeoyl tyramine showed the highest reducing power (EC50=26μg/ml) compared to the other molecules. The present study found that L. guyonianum amides have effective in vitro antioxidant and radical scavenging activity which can be used in pharmacological and food industry due to their antioxidant properties.
authors.affiliation: Laboratoire Des Plantes Extrêmophile, Centre de Biotechnologie de Borj Cédria, BP 901, 2050 Hammam-Lif, Tunisia; Univ. Bordeaux2, ISVV, Groupe d'Etude des Substances Végétales à Activité Biologique, EA 3675, F-33882 Villenave d'Ornon, France. Electronic address: najltrabe3351@gmail.com.
authors.forename: Najla
authors.initials: N
authors.lastname: Trabelsi
authors.forename: Samia
authors.initials: S
authors.lastname: Oueslati
authors.forename: Riadh
authors.initials: R
authors.lastname: Ksouri
authors.forename: Merian
authors.initials: M
authors.lastname: Nassra
authors.forename: Axel
authors.initials: A
authors.lastname: Marchal
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Krisa
authors.forename: Chedly
authors.initials: C
authors.lastname: Abdelly
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Mérillon
authors.forename: Pierre
authors.initials: P
authors.lastname: Waffo-Téguo
mesh_terms: D000577:Amides / Q000737:chemistry* / Q000302:isolation & purification; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification; D019281:Dimerization; D062385:Hydroxybenzoates / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D029606:Plumbaginaceae / Q000737:chemistry*
chemical_list: D000577:Amides; D000975:Antioxidants; D062385:Hydroxybenzoates; D010936:Plant Extracts; C017616:phenolic acid
keywords: 2,2-Diphenyl-1-picrylhydrazyl; Antioxidant activities; BHA; BHT; Centrifugal partition chromatography; DPPH; EC(50); EtOAc; IC(50); Limoniastramide; Limoniastrum guyonianum; MeCN; MeOH; N-E-caffeoyl tyramine; N-E-feruloyl tyramine; TFA; acetonitrile; butylated hydroxyanisole; butylated hydroxytoluene; effective concentration at which the absorbance was 0.5; ethyl acetate; inhibition concentration at 50 percent; methanol; n-hept; n-heptane; trifluoroacetic acid