title: Mast cell-deficient kit mice develop house dust mite-induced lung inflammation despite impaired eosinophil recruitment.
abstract: BACKGROUND
  Mast cells are implicated in allergic and innate immune responses in asthma, although their role in models using an allergen relevant for human disease is incompletely understood. House dust mite (HDM) allergy is common in asthma patients. Our aim was to investigate the role of mast cells in HDM-induced allergic lung inflammation.
  
  
  METHODS
  Wild-type (Wt) and mast cell-deficient Kit(w-sh) mice on a C57BL/6 background were repetitively exposed to HDM via the airways.
  
  
  RESULTS
  HDM challenge resulted in a rise in tryptase activity in bronchoalveolar lavage fluid (BALF) of Wt mice, indicative of mast cell activation. Kit(w-sh) mice showed a strongly attenuated HDM- induced recruitment of eosinophils in BALF and lung tissue, accompanied by reduced pulmonary levels of the eosinophil chemoattractant eotaxin. Remarkably, Kit(w-sh) mice demonstrated an unaltered capacity to develop lung pathology and increased mucus production in response to HDM. The increased plasma IgE in response to HDM in Wt mice was absent in Kit(w-sh) mice.
  
  
  CONCLUSIONS
  These data contrast with previous reports on the role of mast cells in models using ovalbumin as allergen in that C57BL/6 Kit(w-sh) mice display a selective impairment of eosinophil recruitment without differences in other features of allergic inflammation.
authors.affiliation: Center of Infection and Immunity Amsterdam & Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: J Daan
authors.initials: JD
authors.lastname: de Boer
authors.forename: Jack
authors.initials: J
authors.lastname: Yang
authors.forename: Florry E
authors.initials: FE
authors.lastname: van den Boogaard
authors.forename: Arie J
authors.initials: AJ
authors.lastname: Hoogendijk
authors.forename: Regina
authors.initials: R
authors.lastname: de Beer
authors.forename: Jaring S
authors.initials: JS
authors.lastname: van der Zee
authors.forename: Joris J T H
authors.initials: JJ
authors.lastname: Roelofs
authors.forename: Cornelis
authors.initials: C
authors.lastname: van 't Veer
authors.forename: Alex F
authors.initials: AF
authors.lastname: de Vos
authors.forename: Tom
authors.initials: T
authors.lastname: van der Poll
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D001249:Asthma / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology / Q000276:immunology; D054413:Chemokine CCL11 / Q000276:immunology / Q000378:metabolism; D004195:Disease Models, Animal; D004804:Eosinophils / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007150:Immunohistochemistry; D008168:Lung / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008407:Mast Cells / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009093:Mucus / Q000276:immunology / Q000378:metabolism; D010047:Ovalbumin / Q000276:immunology; D011014:Pneumonia / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D039981:Pyroglyphidae / Q000276:immunology*; D053802:Tryptases / Q000276:immunology / Q000378:metabolism
chemical_list: D000485:Allergens; D054413:Chemokine CCL11; D007073:Immunoglobulin E; D010047:Ovalbumin; D019009:Proto-Oncogene Proteins c-kit; D053802:Tryptases