title: [Dynamics of Cry1ab protein content in the rhizosphere soil and straw debris of transgenic Bt corn].
abstract: By using ELISA test kits, a field investigation was conducted on the degradation dynamics of CrylAb protein in the rhizosphere soil of Bt corn MON810 at its different growth stages and in the MON810 straws returned into field after harvest. Three models (shift-log model, exponential model, and bi-exponential model) were used to fit the degradation dynamics of the Cry1 Ab protein from the straw debris, and the DT50 and DT90, values were estimated. There existed great differences in the CrylAb protein content in the rhizosphere soil of MON810 at its different growth stages, but overall, the CrylAb protein content was decreased remarkably with the growth of MON810. The degradation of Cry1 Ab protein from the straws covered on soil surface and buried in soil showed the same two-stage pattern, i.e., more rapid at early stage and slow-stable in later period. Within the first week after straw return, the degradation rate of the CrylAb protein from the straws covered on soil surface was significantly higher than that from the straws buried in soil. At 10 d, the degradation rate of the CrylAb protein from the straws covered on soil surface and buried in soil was basically the same, being 88.8% and 88.6%, respectively. After 20 days, the degradation of CrylAb protein entered slow-stable stage. Till at 180 d, a small amount of Cry1Ab protein could still be detected in the straw debris. All of the three models used in this study could fit the decay pattern of the CrylAb protein from the straw debris in field. By comparing the correlation coefficient (r) and the consistency between the measured and calculated DT90, bi-exponential model was considered to be the best.
authors.affiliation: Institute of Plant Protection, Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Plant Virology, Ji'nan 250100, China. lfzjnd@163.com
authors.forename: Fan
authors.initials: F
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Sun
authors.forename: Shu-Ke
authors.initials: SK
authors.lastname: Yang
authors.forename: Xing-Bo
authors.initials: XB
authors.lastname: Lu
mesh_terms: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000378:metabolism*; D017753:Ecosystem; D004731:Endotoxins / Q000378:metabolism*; D005308:Fertilizers; D006460:Hemolysin Proteins / Q000378:metabolism*; D010572:Pest Control, Biological; D018517:Plant Roots / Q000378:metabolism; D018547:Plant Stems / Q000737:chemistry*; D030821:Plants, Genetically Modified / Q000378:metabolism*; D012037:Refuse Disposal / Q000379:methods; D058441:Rhizosphere; D012987:Soil / Q000737:chemistry; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D005308:Fertilizers; D006460:Hemolysin Proteins; D012987:Soil; C030956:insecticidal crystal protein, Bacillus Thuringiensis