title: Molecular identification of Ancylostoma caninum isolated from cats in southern China based on complete ITS sequence.
abstract: Ancylostoma caninum is a blood-feeding parasitic intestinal nematode which infects dogs, cats, and other mammals throughout the world. A highly sensitive and species-specific PCR-RFLP technique was utilised to detect the prevalence of A. caninum in cats in Guangzhou, southern China. Of the 102 fecal samples examined, the prevalence of A. caninum in cats was 95.1% and 83.3% using PCR-RFLP and microscopy, respectively. Among them, the prevalence of single hookworm infection with A. caninum was 54.90%, while mixed infections with both A. caninum and A. ceylanicum were 40.20%. Comparative analysis of three complete ITS sequences obtained from cat-derived A. caninum showed the same length (738 bp) as that of dog-derived A. caninum. However, the sequence variation range was 98.6%-100%, where only one cat isolate (M63) showed 100% sequence similarity in comparison with two dog-derived A. caninum isolates (AM850106, EU159416) in the same studied area. The phylogenetic tree revealed A. caninum derived from both cats and dogs in single cluster. Results suggest that cats could be the main host of A. caninum in China, which may cause cross-infection between dogs and cats in the same area.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Yuanjia
authors.initials: Y
authors.lastname: Liu
authors.forename: Guochao
authors.initials: G
authors.lastname: Zheng
authors.forename: Muhamd
authors.initials: M
authors.lastname: Alsarakibi
authors.forename: Xinheng
authors.initials: X
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.forename: Pengyun
authors.initials: P
authors.lastname: Lu
authors.forename: Liqin
authors.initials: L
authors.lastname: Lin
authors.forename: Liping
authors.initials: L
authors.lastname: Tan
authors.forename: Qin
authors.initials: Q
authors.lastname: Luo
authors.forename: Guoqing
authors.initials: G
authors.lastname: Li
mesh_terms: D000722:Ancylostoma / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D000724:Ancylostomiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D000818:Animals; D002371:Cat Diseases / Q000453:epidemiology / Q000469:parasitology*; D002415:Cats; D002681:China / Q000453:epidemiology; D004285:Dogs; D005243:Feces / Q000469:parasitology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D018510:RNA, Helminth / Q000235:genetics* / Q000302:isolation & purification; D013045:Species Specificity
chemical_list: D018510:RNA, Helminth