title: The differences in colonic mucosal microbiota between normal individual and colon cancer patients by polymerase chain reaction-denaturing gradient gel electrophoresis.
abstract: OBJECTIVE
  The aim of this study was to analyze the differences in the intestinal composition between normal individuals and colon cancer patients.
  
  
  METHODS
  To establish the criteria for screening a normal individual for colon cancer, human colonic biopsies were obtained at routine colonoscopy. For patients with colon cancer, samples were obtained from cancerous regions. For normal individuals, colonic biopsies were taken from 3 sites of large intestine (descending, transverse, and ascending colon). Thereafter, a comparison of the microbiota structure by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was carried out. At last, bacterial species were identified by sequencing special bands from DGGE gels and comparing data with sequence databases.
  
  
  RESULTS
  With PCR-DGGE, we have discovered that the diversity and richness of the bacterial community from colon cancer patient's colonic mucosa were lower than that of the normal individual's sample. Then, a special DGGE band was found in the colon cancer patients. After sequencing, we confirmed that it had a high level of similarity with bacteroides.
  
  
  CONCLUSIONS
  Colon cancers are closely related with the alteration of intestinal flora such as the reduction of biodiversity and richness of the bacterial community. Furthermore, the increase in proportion of bacteroides may be directly associated with colon cancer.
authors.affiliation: Department of General Surgery, The 5th People's Hospital of Shanghai, Fudan University, Shanghai, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Huipeng
authors.forename: Gong
authors.initials: G
authors.lastname: Lifeng
authors.forename: Ge
authors.initials: G
authors.lastname: Chuang
authors.forename: Zhao
authors.initials: Z
authors.lastname: Jiaying
authors.forename: Cai
authors.initials: C
authors.lastname: Yuankun
mesh_terms: D000328:Adult; D000368:Aged; D001439:Bacteroides / Q000302:isolation & purification*; D002277:Carcinoma / Q000382:microbiology*; D016022:Case-Control Studies; D003106:Colon / Q000382:microbiology*; D044682:Colon, Ascending / Q000382:microbiology; D044683:Colon, Descending / Q000382:microbiology; D044684:Colon, Transverse / Q000382:microbiology; D003110:Colonic Neoplasms / Q000382:microbiology*; D003113:Colonoscopy; D058645:Denaturing Gradient Gel Electrophoresis; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction