title: Comparative proteomics of peanut gynophore development under dark and mechanical stimulation.
abstract: Peanut (Arachis hypogaea. L) is an important leguminous crop and source of proteins and lipids. It has attracted widespread attention of researchers due to its unique growth habit of geocarpy, which is regulated by geotropism, negative phototropism, and haptotropism. However, the protein expression pattern and molecular regulatory mechanism underlying the physiological processes of peanut remain unknown. In this study, the peanut gynophores under five treatment conditions were used for proteomic analysis, including aerial growth of the gynophores, the gynophores penetrated into the soil, as well as aerial growth of the gynophores under mechanical stimulation, dark, and mechanical stimulation combined with dark. The analysis of protein abundances in peanut gynophores under these conditions were conducted using comparative proteomic approaches. A total of 27 differentially expressed proteins were identified and further classified into nine biological functional groups of stress and defense, carbohydrate and energy metabolism, metabolism, photosynthesis, cell structure, signaling, transcription, protein folding and degradation, and function unknown. By searching gene functions against peanut database, 10 genes with similar annotations were selected as corresponding changed proteins, and their variation trends in gynophores under such growth conditions were further verified using quantitative real-time PCR. Overall, the investigation will benefit to enrich our understanding of the internal mechanisms of peanut gynophore development and lay a foundation for breeding and improving crop varieties and qualities.
authors.affiliation: Biotechnology Research Center, Shandong Academy of Agricultural Sciences , Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops, 202# North of Gongye Road, Ji'nan, Shandong 250100, P. R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Sun
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Wang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Hou
authors.forename: Shaojun
authors.initials: S
authors.lastname: Dai
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
mesh_terms: D010367:Arachis / Q000235:genetics* / Q000378:metabolism; D001696:Biomechanical Phenomena; D003624:Darkness; D035264:Flowers / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D040542:Mechanotransduction, Cellular; D053858:Metabolic Networks and Pathways; D058977:Molecular Sequence Annotation; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D040901:Proteomics; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D010940:Plant Proteins