title: Protective effect of spin-labeled 1-ethyl-1-nitrosourea against oxidative stress in liver induced by antitumor drugs and radiation.
abstract: This study was carried out to investigate possible protection effect of 1-ethyl-3-[4-(2,2,6,6-tetramethylpiperidine-1-oxyl)]-1-nitrosourea (SLENU), synthesized in our laboratory, against oxidative liver injuries induced in mice treated by antitumor drugs: doxorubicin (DOX), bleomycin (BLM), or gamma irradiation (R). Specifically, alterations in some biomarkers of oxidative stress, such as lipid peroxidation products measured as malondialdehyde (MDA) levels and activities of the antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT), were studied in liver homogenates isolated from tumor bearing C57 black mice after i.p. treatment with solutions of DOX (60 mg/kg), BLM (60 mg/kg), or after total body gamma-irradiation with a single dose of 5 Gy. The same biomarkers were also measured after i.p. pretreatment of mice with SLENU (100 mg/kg). Statistical significant increased MDA levels and SOD and CAT enzymes activities were found in the liver homogenates of tumor bearing mice after alone treatment with DOX or gamma-irradiation compared to the control mice, while these parameters were insignificantly increased after BLM administration compared to the same controls.
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Medicine, Trakia University, Armeiska Street 11, 6000 Stara Zagora, Bulgaria.
authors.forename: V
authors.initials: V
authors.lastname: Gadjeva
authors.forename: B
authors.initials: B
authors.lastname: Grigorov
authors.forename: G
authors.initials: G
authors.lastname: Nikolova
authors.forename: A
authors.initials: A
authors.lastname: Tolekova
authors.forename: A
authors.initials: A
authors.lastname: Zheleva
authors.forename: M
authors.initials: M
authors.lastname: Vasileva
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001219:Aspartate Aminotransferases / Q000097:blood; D001761:Bleomycin / Q000633:toxicity; D002374:Catalase / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control; D004317:Doxorubicin / Q000633:toxicity; D005038:Ethylnitrosourea / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D005720:Gamma Rays / Q000009:adverse effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism* / Q000528:radiation effects; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects; D026361:Reactive Nitrogen Species / Q000378:metabolism; D013113:Spin Labels; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D026361:Reactive Nitrogen Species; D013113:Spin Labels; D001761:Bleomycin; D008315:Malondialdehyde; D004317:Doxorubicin; D002374:Catalase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005038:Ethylnitrosourea