title: In vitro digestion combined with cellular assay to determine the antioxidant activity in Chinese bayberry (Myrica rubra Sieb. et Zucc.) fruits: a comparison with traditional methods.
abstract: The traditional method of chemical extraction (i.e., extracts), combined with chemical antioxidant activity assays cannot assess the real antioxidant activity. In vitro digestion (i.e., digesta) with a cellular antioxidant activity (CAA) assay was developed for the determination of antioxidant activity in Chinese bayberry fruits. In this study, pretreatment methods were studied and the results showed that digesta had more free phenolic acids (FPA) but less total phenolic content (TPC) and total anthocyanin content (TAC) than extracts. Antioxidant activity assays, including ABTS, FRAP, DPPH, ORAC and CAA, were compared. Digesta had lower ABTS, FRAP and DPPH values but higher CAA values than extracts. FPA were better correlated with the chemical antioxidant assays in digesta. The correlations were high between TPC and CAA values in digesta (R(2)=0.96) but not extracts (R(2)=0.58). Higher correlations were also obtained between CAA and chemical assays in digesta.
authors.affiliation: Department of Food Science and Nutrition, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou 310058, China.
authors.forename: Haizhi
authors.initials: H
authors.lastname: Huang
authors.forename: Yujing
authors.initials: Y
authors.lastname: Sun
authors.forename: Shuting
authors.initials: S
authors.lastname: Lou
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Xingqian
authors.initials: X
authors.lastname: Ye
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism; D001681:Biological Assay / Q000379:methods*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005638:Fruit / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D031641:Myrica / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts
keywords: Anthocyanins; Cellular antioxidant activity (CAA); Chinese bayberry fruit; In vitro digestion; Phenolics