title: Detection of Leptospira-specific antibodies using a recombinant antigen-based enzyme-linked immunosorbent assay.
abstract: We produced three highly purified recombinant antigens rLipL32, rLipL41, and rLigA-Rep (leptospiral immunoglobulin-like A repeat region) for the detection of Leptospira-specific antibodies in an enzyme-linked immunosorbent assay (ELISA). The performance of these recombinant antigens was evaluated using 121 human sera. Among them, 63 sera were microscopic agglutination test (MAT)-confirmed positive sera from febrile patients in Peru, 22 sera were indigenous MAT-negative febrile patient sera, and 36 sera were from patients with other febrile diseases from Southeast Asia, where leptospirosis is also endemic. Combining the results of immunoglobulin M (IgM) and IgG detection from these three antigens, the overall sensitivity is close to 90% based on the MAT. These results suggest that an ELISA using multiple recombinant antigens may be used as an alternative method for the detection of Leptospira-specific antibodies.
authors.affiliation: Naval Medical Research Center, Silver Spring, Maryland; Naval Medical Research Unit No.6, Lima, Peru; Naval Medical Center San Diego, San Diego, California.
authors.forename: Hua-Wei
authors.initials: HW
authors.lastname: Chen
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Eric S
authors.initials: ES
authors.lastname: Halsey
authors.forename: Carolina
authors.initials: C
authors.lastname: Guevara
authors.forename: Enrique
authors.initials: E
authors.lastname: Canal
authors.forename: Eric
authors.initials: E
authors.lastname: Hall
authors.forename: Ryan
authors.initials: R
authors.lastname: Maves
authors.forename: Drake H
authors.initials: DH
authors.lastname: Tilley
authors.forename: Tadeusz J
authors.initials: TJ
authors.lastname: Kochel
authors.forename: Wei-Mei
authors.initials: WM
authors.lastname: Ching
mesh_terms: D000372:Agglutination Tests; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007919:Leptospira / Q000276:immunology* / Q000302:isolation & purification; D007922:Leptospirosis / Q000175:diagnosis* / Q000382:microbiology; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism; D010568:Peru; D011994:Recombinant Proteins / Q000276:immunology; D013045:Species Specificity
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C406589:LipL32 protein, Leptospira; C538745:LipL41 protein, Leptospira interrogans; D008074:Lipoproteins; D011994:Recombinant Proteins