title: Cibacron blue immobilized poly(glycidyl-methacrylate) nanobeads for albumin removal in proteome studies.
abstract: A new approach to albumin removal for proteome studies involving human plasma samples was presented with dye affinity poly glycidyl methacrylate (PGMA) nanobeads in an average size of 45 nm. The specific surface area of PGMA nanobeads was calculated as 2616 m(2)/g. Cibacron Blue F3GA (CB) was immobilized onto PGMA nanobeads as dye ligand, and CB immobilized PGMA (CB-PGMA) nanobeads were characterized by a serial processing. Albumin depletion efficiency of CB-PGMA nanobeads was investigated in human plasma sample and confirmed by two-dimensional gel electrophoresis.
authors.affiliation: Department of Chemistry, Hacettepe University , Beytepe, Ankara , Turkey.
authors.forename: Müge
authors.initials: M
authors.lastname: Andaç
mesh_terms: D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D040901:Proteomics; D012709:Serum Albumin / Q000737:chemistry / Q000302:isolation & purification*; D014227:Triazines / Q000737:chemistry*
chemical_list: D011109:Polymethacrylic Acids; D012709:Serum Albumin; D014227:Triazines; C042535:polyglycidyl methacrylate; C012842:Cibacron Blue F 3GA
keywords: albumin depletion; dye affinity; poly glycidyl methacrylate nanobeads; proteomics