title: Alveolar epithelial cells (A549) exposed at the air-liquid interface to diesel exhaust: First study in TNO's powertrain test center.
abstract: Air-liquid interface (ALI) exposures enable in vitro testing of mixtures of gases and particles such as diesel exhaust (DE). The main objective of this study was to investigate the feasibility of exposing human lung epithelial cells at the ALI to complete DE generated by a heavy-duty truck in the state-of-the-art TNO powertrain test center. A549 cells were exposed at the air-liquid interface to DE generated by a heavy-duty Euro III truck for 1.5h. The truck was tested at a speed of ∼70kmh(-1) to simulate free-flowing traffic on a motorway. Twenty-four hours after exposure, cells were analyzed for markers of oxidative stress (GSH and HO-1), cytotoxicity (LDH and Alamar Blue assay) and inflammation (IL-8). DE exposure resulted in an increased oxidative stress response (significantly increased HO-1 levels and significantly reduced GSH/GSSH ratio), and a decreased cell viability (significantly decreased Alamar Blue levels and slightly increased LDH levels). However, the pro-inflammatory response seemed to decrease (decrease in IL-8). The results presented here demonstrate that we are able to successfully expose A549 cells at ALI to complete DE generated by a heavy-duty truck in TNO's powertrain test center and show oxidative stress and cytotoxicity responses due to DE exposure.
authors.affiliation: The Netherlands Organisation for Applied Scientific Research, TNO, P.O. Box 80015, 3508 TA Utrecht, The Netherlands. Electronic address: ingeborg.kooter@tno.nl.
authors.forename: Ingeborg M
authors.initials: IM
authors.lastname: Kooter
authors.forename: Marcel J
authors.initials: MJ
authors.lastname: Alblas
authors.forename: Aleksandra D
authors.initials: AD
authors.lastname: Jedynska
authors.forename: Maaike
authors.initials: M
authors.lastname: Steenhof
authors.forename: Marc M G
authors.initials: MM
authors.lastname: Houtzager
authors.forename: Martijn
authors.initials: M
authors.lastname: van Ras
mesh_terms: D000388:Air; D000393:Air Pollutants / Q000633:toxicity*; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004847:Epithelial Cells; D005978:Glutathione / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000378:metabolism; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007753:Laboratories; D010078:Oxazines / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000166:cytology; D018675:Toxicity Tests / Q000379:methods*; D001335:Vehicle Emissions / Q000633:toxicity*; D014966:Xanthenes / Q000378:metabolism
chemical_list: D000393:Air Pollutants; D016209:Interleukin-8; D010078:Oxazines; D001335:Vehicle Emissions; D014966:Xanthenes; C005843:resazurin; D007770:L-Lactate Dehydrogenase; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1; D005978:Glutathione
keywords: Air–liquid interface; Climatic-altitude chamber; Diesel exhaust; In vitro; Oxidative stress; Real world emission