title: Development of anodic titania nanotubes for application in high sensitivity amperometric glucose and uric acid biosensors.
abstract: The purpose of this study was to develop novel nanoscale biosensors using titania nanotubes (TNTs) made by anodization. Titania nanotubes were produced on pure titanium sheets by anodization at room temperature. In this research, the electrolyte composition ethylene glycol 250 mL/NH4F 1.5 g/DI water 20 mL was found to produce the best titania nanotubes array films for application in amperometric biosensors. The amperometric results exhibit an excellent linearity for uric acid (UA) concentrations in the range between 2 and 14 mg/dL, with 23.3 (µA·cm-2)·(mg/dL)-1 UA sensitivity, and a correlation coefficient of 0.993. The glucose biosensor presented a good linear relationship in the lower glucose concentration range between 50 and 125 mg/dL, and the corresponding sensitivity was approximately 249.6 (µA·cm-2)·(100 mg/dL)-1 glucose, with a correlation coefficient of 0.973.
authors.affiliation: Department of Mechatronic Engineering, Huafan University, Shihding, Taipei Hsien 223-01, Taiwan. cllin@huafan.hfu.edu.tw.
authors.forename: Hsiang-Ching
authors.initials: HC
authors.lastname: Lee
authors.forename: Li-Fan
authors.initials: LF
authors.lastname: Zhang
authors.forename: Jyh-Ling
authors.initials: JL
authors.lastname: Lin
authors.forename: Yuan-Lung
authors.initials: YL
authors.lastname: Chin
authors.forename: Tai-Ping
authors.initials: TP
authors.lastname: Sun
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014025:Titanium / Q000737:chemistry*; D014159:Transducers; D014527:Uric Acid / Q000032:analysis*
chemical_list: C009495:titanium dioxide; D014527:Uric Acid; D014025:Titanium; D005947:Glucose