title: A new approach to bacterial colony morphotyping by matrix-assisted laser desorption ionization time of flight-based mass spectrometry.
abstract: Matrix assisted laser desorption ionization time of flight mass spectrometry has been explored as a tool to bacterial colony morphotyping. To this end, four colony morphotypes of Pseudomonas aeruginosa and four of Staphylococcus aureus were analysed using intact bacteria. Results suggest that mass spectrometry of intact bacteria could, in some extent, be used to complement the classical morphological classification of bacteria.
authors.affiliation: Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Ana Margarida
authors.initials: AM
authors.lastname: Sousa
authors.forename: J D
authors.initials: JD
authors.lastname: Nunes-Miranda
authors.forename: Miguel
authors.initials: M
authors.lastname: Reboiro-Jato
authors.forename: Florentino
authors.initials: F
authors.lastname: Fdez-Riverola
authors.forename: Anália
authors.initials: A
authors.lastname: Lourenço
authors.forename: Maria Olívia
authors.initials: MO
authors.lastname: Pereira
authors.forename: J L
authors.initials: JL
authors.lastname: Capelo
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D018441:Biofilms; D010802:Phylogeny; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000648:ultrastructure; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013211:Staphylococcus aureus / Q000145:classification* / Q000648:ultrastructure
keywords: Bacteria; Classification; MALDI; Morphotype