title: Using RAPDs to study phylogenetic relationships in Rosa.
abstract: Nineteen species of rose (Rosa sp.) were analysed using Random Amplified Polymorphic DNA markers (RAPD). Each 10-base-long arbitrary primer produced a specific DNA banding pattern that grouped plants belonging to the same species and botanical sections as predicted from their genetic background. One hundred and seventy-five amplification products were examined by cluster analysis to assess the genetic relationships among species and their genetic distances. All of the accessions belonging to 1 species grouped together before branching to other species. Dendrograms constructed for intra- and inter-specific studies showed a good correlation with previous classifications by different authors based on morphological and cariological studies. Our results show that the RAPD technique is a sensitive and precise tool for genomic analysis in rose, being useful in assigning unclassified accessions to specific taxonomic groups or else allowing accessions classified by traditional criteria to be re-classified.
authors.affiliation: Departamento de Genética, E.T.S.I.A.M., Aptdo. 3048, 14080, Córdoba, Spain.
authors.forename: T
authors.initials: T
authors.lastname: Millan
authors.forename: F
authors.initials: F
authors.lastname: Osuna
authors.forename: S
authors.initials: S
authors.lastname: Cobos
authors.forename: A M
authors.initials: AM
authors.lastname: Torres
authors.forename: J I
authors.initials: JI
authors.lastname: Cubero