title: Translational regulation of Yersinia enterocolitica mRNA encoding a type III secretion substrate.
abstract: Yersinia enterocolitica type III secretion machines transport YopQ and other Yop effectors into host immune cells. YopD and its chaperone LcrH are essential components of the Yersinia type III pathway, enabling effector translocation into host cells. YopD, LcrH, and YscM1 also regulate yop expression post-transcriptionally in response to environmental signals; however, the molecular mechanisms for this regulation and Yop secretion are unknown. We show here that YopD associates with 30 S ribosomal particles in a manner requiring LcrH. When added to ribosomes, YopD, LcrH, and YscM1 block the translation of yopQ mRNA. We propose a model whereby LcrH-dependent association of YopD with 30 S ribosomal particles enables YscM1 to block yopQ translation unless type III machines are induced to secrete the effector.
authors.affiliation: From the Howard Taylor Ricketts Laboratory, Argonne National Laboratory, Lemont, Illinois 60439 and the Department of Microbiology, University of Chicago, Chicago, Illinois 60637.
authors.forename: Karyl S
authors.initials: KS
authors.lastname: Kopaskie
authors.forename: Katherine Given
authors.initials: KG
authors.lastname: Ligtenberg
authors.forename: Olaf
authors.initials: O
authors.lastname: Schneewind
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D004843:Epistasis, Genetic; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008954:Models, Biological; D018832:Molecular Chaperones / Q000235:genetics* / Q000378:metabolism*; D014176:Protein Biosynthesis; D021381:Protein Transport; D012329:RNA, Bacterial / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D054680:Ribosome Subunits, Small, Bacterial / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D018832:Molecular Chaperones; D012329:RNA, Bacterial; D012333:RNA, Messenger; C118339:SycD protein, bacteria; D014157:Transcription Factors; C078910:YopD protein, Yersinia; C110346:YscM1 protein, Yersinia enterocolitica; C067570:YopQ protein, Yersinia enterocolitica
keywords: Bacterial Pathogenesis; Ribosomes; Secretion; Translation Regulation; mRNA