title: Cysteinyl leukotriene levels correlate with 8-isoprostane levels in exhaled breath condensates of atopic and healthy children.
abstract: BACKGROUND
  Cysteinyl leukotrienes are important mediators of airway inflammation, whereas 8-isoprostane is a biomarker of oxidative stress. This study evaluated the distributions of cysteinyl leukotriene and 8-isoprostane concentrations in exhaled breath condensates (EBCs) of children. The relationship between cysteinyl leukotriene and 8-isoprostane concentrations in the EBCs was also evaluated.
  
  
  METHODS
  The EBCs were collected from 34 children with allergic respiratory diseases and 24 healthy children. All recruited children underwent pulmonary function testing every season. The severity of allergic respiratory diseases and medication status were assessed every month in children with allergic respiratory diseases.
  
  
  RESULTS
  The EBC cysteinyl leukotriene and 8-isoprostane levels were higher in children with asthma and allergic rhinitis than in those with asthma only and healthy children. In asthmatic children, cysteinyl leukotriene and 8-isoprostane levels peaked in the summer. All children showed a clear association between EBC cysteinyl leukotriene and EBC 8-isoprostane levels.
  
  
  CONCLUSIONS
  The cysteinyl leukotriene and 8-isoprostane concentrations in the EBCs of children significantly varied by season. Oxidative stress correlated with airway inflammation in children.
authors.affiliation: Department of Respiratory Therapy, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan.
authors.forename: Gwo-Hwa
authors.initials: GH
authors.lastname: Wan
authors.forename: Dah-Chin
authors.initials: DC
authors.lastname: Yan
authors.forename: Hui-Yun
authors.initials: HY
authors.lastname: Tseng
authors.forename: Tao-Hsin
authors.initials: TH
authors.lastname: Tung
authors.forename: Syh-Jae
authors.initials: SJ
authors.lastname: Lin
authors.forename: Yu-Wen
authors.initials: YW
authors.lastname: Lin
mesh_terms: D001944:Breath Tests; D002648:Child; D003545:Cysteine / Q000032:analysis*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000032:analysis; D006801:Humans; D015289:Leukotrienes / Q000032:analysis*; D016014:Linear Models; D018384:Oxidative Stress / Q000502:physiology; D012129:Respiratory Function Tests; D012130:Respiratory Hypersensitivity / Q000378:metabolism*; D012621:Seasons; D013624:Taiwan
chemical_list: D015289:Leukotrienes; C112381:cysteinyl-leukotriene; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost; D003545:Cysteine