title: Neuroprotective effects of LBP on brain ischemic reperfusion neurodegeneration.
abstract: OBJECTIVE
  The present study was conducted to investigate whether LBP had a protective effect on cerebral ischemic reperfusion injury and to determine the possible mechanisms.
  
  
  METHODS
  Male Kunming (KM) mice were used to make the model cerebral artery occlusion/reperfusion (MCAO/R). The behavioral test was used to measure neurological deficit scores for evaluation of ischemic reperfusion damage of brain. The change of electroencephalograph (EEG) was monitored by Model SMUP-E Bio-electric Signals Processing System. The infarction area of brain was assessed in brain slices with 2% solution of 2,3,5-triphenyl tetrazolium chloride (TTC). Spectrophotometric assay was used to determine the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and lactate dehydrogenase (LDH), contents of malondialdehyde (MDA) and adenosine triphosphate (ATP) of the brain.
  
  
  RESULTS
  The results showed that LBP at doses of 20 and 40 mg/kg markedly decreased the neurological deficit scores and the infarction area in MCAO/R mice. At the same time, LBP significantly decreased MDA content, and increased SOD, GSH-Px, CAT, LDH activities in ischemic reperfusion brain.
  
  
  CONCLUSIONS
  These suggest that LBP might act as a potential neuroprotective agent against the cerebral reperfusion-induced injury in the brain through reducing lipid peroxides, scavenging free radicals, and improving the energy metabolism.
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Yinchuan, China. yujq910315@163.com.
authors.forename: H-B
authors.initials: HB
authors.lastname: Wang
authors.forename: Y-X
authors.initials: YX
authors.lastname: Li
authors.forename: Y-J
authors.initials: YJ
authors.lastname: Hao
authors.forename: T-F
authors.initials: TF
authors.lastname: Wang
authors.forename: Z
authors.initials: Z
authors.lastname: Lei
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.forename: Q-P
authors.initials: QP
authors.lastname: Zhao
authors.forename: H
authors.initials: H
authors.lastname: Ang
authors.forename: L
authors.initials: L
authors.lastname: Ma
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: C-J
authors.initials: CJ
authors.lastname: Zhao
authors.forename: Y-X
authors.initials: YX
authors.lastname: Jiang
authors.forename: Y-R
authors.initials: YR
authors.lastname: Wang
authors.forename: X-Y
authors.initials: XY
authors.lastname: Dai
authors.forename: W-N
authors.initials: WN
authors.lastname: Zhang
authors.forename: T
authors.initials: T
authors.lastname: Sun
authors.forename: J-Q
authors.initials: JQ
authors.lastname: Yu
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004569:Electroencephalography / Q000187:drug effects; D008297:Male; D051379:Mice; D018696:Neuroprotective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000517:prevention & control*
chemical_list: D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; C500223:lycium barbarum polysaccharide