title: The genetic fine structure of nonsense suppressors in Schizosaccharomyces pombe : II. sup8 and sup10.
abstract: Meiotic fine-structure maps of two efficient UGA suppressors of Schizosaccharomyces pombe which are known (sup8-e) or inferred (sup10-e) to code for two leucine tRNAs carrying the mutant anticodon U(*)CA (Kohli et al. 1979, 1980a, b; Wetzel et al. 1979; Mao et al. 1981) are presented. In both cases, the recombination frequencies given by the primary site of the anticodon mutation fitwell into the map defined by the sites of a number of inactivating secondary mutations. This contrasts the corresponding situation found in the serine tRNA genes sup3 and sup9 where the anticodon site exhibits a specific marker effect which strongly increases recombination frequencies in crosses with all revertant sites, due to a decrease in the efficiency of excision repair of base-pair mismatches whenever the anticodon site is included in hybrid-DNA (Hofer et al. 1979; Munz and Leupold 1979; Thuriaux et al. 1980). A pronounced specific marker effect which leads to a several fold increase of the recombination frequencies over those expected is observed, however, at one of the secondary inactivating sites mapping in the leucine tRNA gene sup8.
authors.affiliation: Institute of General Microbiology, University of Bern, Baltzer-Strasse 4, CH-3012, Bern, Switzerland.
authors.forename: P
authors.initials: P
authors.lastname: Munz
authors.forename: K
authors.initials: K
authors.lastname: Dorsch-Häsler
authors.forename: U
authors.initials: U
authors.lastname: Leupold