title: Drosophila germline stem cells for in vitro analyses of PIWI-mediated RNAi.
abstract: The Drosophila piwi gene has multiple functions in soma and germ cells. An in vitro system provides a powerful tool for elucidating PIWI function in each cell type using stable cell lines originating from germline stem cells (GSCs) and ovarian soma of adult ovaries. We have described methods for the maintenance and expansion of GSCs in an established cell line (fGS/OSS) and an in situ hybridization method for analyzing piwi.
authors.affiliation: Department of Biology, Faculty of Science, Ibaraki University, Mito, Ibaraki, Japan.
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Niki
authors.forename: Takuya
authors.initials: T
authors.lastname: Sato
authors.forename: Takafumi
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Ayaka
authors.initials: A
authors.lastname: Saisho
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uetake
authors.forename: Hidenori
authors.initials: H
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D060565:Argonaute Proteins / Q000172:deficiency / Q000235:genetics*; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media; D029721:Drosophila Proteins / Q000172:deficiency / Q000235:genetics*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000235:genetics*; D005854:Germ Cells / Q000166:cytology*; D017403:In Situ Hybridization; D008297:Male; D034622:RNA Interference; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D060565:Argonaute Proteins; D003470:Culture Media; D029721:Drosophila Proteins; C116498:piwi protein, Drosophila