title: Concanavalin A-mediated T cell proliferation is regulated by herpes virus entry mediator costimulatory molecule.
abstract: T cell activation is regulated by two distinct signals, signals one and two. Concanavalin A (ConA) is an antigen-independent mitogen and functions as signal one inducer, leading T cells to polyclonal proliferation. CD28 is known to be one of major costimulatory receptors and to provide signal two in the ConA-induced T cell proliferation. Here, we have studied the implication of other costimulatory pathways in the ConA-mediated T cell proliferation by using soluble recombinant proteins consisting of an extracellular domain of costimulatory receptors and Fc portion of human IgG. We found that T cell proliferation induced by ConA, but not PMA plus ionomycin or anti-CD3 mAb, is significantly inhibited by herpes virus entry mediator (HVEM)-Ig, even in the presence of CD28 signaling. Moreover, the high concentration of HVEM-Ig molecules almost completely suppressed ConA-mediated T cell proliferation. These results suggest that HVEM might play more important roles than CD28 in ConA-mediated T cell proliferation.
authors.affiliation: Laboratory of Human Molecular Genetics, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa, 920-1192, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Ando
authors.forename: Chika
authors.initials: C
authors.lastname: Yasuoka
authors.forename: Takuya
authors.initials: T
authors.lastname: Mishima
authors.forename: Takuya
authors.initials: T
authors.lastname: Ikematsu
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Uede
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Matsunaga
authors.forename: Manabu
authors.initials: M
authors.lastname: Inobe
mesh_terms: D018106:CD28 Antigens / Q000276:immunology* / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D003208:Concanavalin A / Q000008:administration & dosage* / Q000276:immunology; D006568:Herpesvirus 1, Cercopithecine / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D053240:Receptors, Tumor Necrosis Factor, Member 14 / Q000276:immunology / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism
chemical_list: D018106:CD28 Antigens; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D053240:Receptors, Tumor Necrosis Factor, Member 14; D003208:Concanavalin A