title: Immunostimulation by poly-β hydroxybutyrate-hydroxyvalerate (PHB-HV) from Bacillus thuringiensis in Oreochromis mossambicus.
abstract: The present study was designed to test the immunostimulatory efficacy of poly-β hydroxybutyrate-hydroxyvalerate (PHB-HV) extracted from Bacillus thuringiensis B.t.A102 on the immune system of Oreochromis mossambicus. Fish were fed with 0%, 1%, 3% or 5% PHB-HV supplemented feed and were bled at regular intervals of 5 days. The specific immune response was measured in terms of antibody response to sheep red blood cells, the nonspecific immune mechanisms were analysed in terms of serum lysozyme activity, total peroxidases activity and antiprotease activity. The overall functional immunity was tested by experimental challenge with live virulent Aeromonas hydrophila. The results revealed that all the doses of PHB-HV supplementation in feed were effective in stimulating both specific and nonspecific immune mechanisms. The bacterial challenge experiment showed that highest dose of 5% PHB-HV supplementation was more effective than 1% and 3% doses. The study concludes that PHB-HV can be used as a potential immunostimulant in finfish aquaculture.
authors.affiliation: Postgraduate and Research Department of Zoology and Biotechnology, Lady Doak College, Madurai 625002, Tamil Nadu, India.
authors.forename: Ponnusamy
authors.initials: P
authors.lastname: Suguna
authors.affiliation: Department of Zoology, Thiagarajar College, Madurai 625009, Tamil Nadu, India.
authors.forename: Chandrasekaran
authors.initials: C
authors.lastname: Binuramesh
authors.affiliation: Postgraduate and Research Department of Zoology and Biotechnology, Lady Doak College, Madurai 625002, Tamil Nadu, India.
authors.forename: Periyasamy
authors.initials: P
authors.lastname: Abirami
authors.affiliation: Postgraduate and Research Department of Zoology and Biotechnology, Lady Doak College, Madurai 625002, Tamil Nadu, India.
authors.forename: Viswanathan
authors.initials: V
authors.lastname: Saranya
authors.affiliation: Department of Zoology, Thiagarajar College, Madurai 625009, Tamil Nadu, India.
authors.forename: Kkani
authors.initials: K
authors.lastname: Poornima
authors.affiliation: Postgraduate and Research Department of Zoology and Biotechnology, Lady Doak College, Madurai 625002, Tamil Nadu, India.
authors.forename: Veluchamy
authors.initials: V
authors.lastname: Rajeswari
authors.affiliation: Postgraduate and Research Department of Zoology and Biotechnology, Lady Doak College, Madurai 625002, Tamil Nadu, India. Electronic address: shenbagarathai@rediffmail.com.
authors.forename: Rajaiah
authors.initials: R
authors.lastname: Shenbagarathai
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology*; D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D001413:Bacillus thuringiensis / Q000737:chemistry*; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology*; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D006386:Hemagglutination Tests / Q000662:veterinary; D007114:Immunization / Q000379:methods; D008297:Male; D009113:Muramidase / Q000097:blood; D009195:Peroxidase / Q000097:blood; D011091:Polyesters / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000097:blood; D017550:Spectroscopy, Fourier Transform Infrared; D017210:Tilapia; D000094122:Polyhydroxybutyrates
chemical_list: D009113:Muramidase; D009195:Peroxidase; D011091:Polyesters; D011480:Protease Inhibitors; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates
keywords: Antiprotease; Dietary supplementation; Lysozyme; Polyhydroxyalkanoate; SCFA