title: In vitro and in vivo survival and colonic adhesion of Pediococcus acidilactici MTCC5101 in human gut.
abstract: The present study aims to investigate the probiotic nature of Pediococcus acidilactici MTCC5101 by an in vitro assay of bacterial adherence to intestinal epithelial cells of human gastrointestinal (GI) tract using Caco-2 cell line. Further to assess the in vivo survival in the GI tract, oral feeding was carried out with the help of 10 healthy volunteers. The effect on wellness was assessed by studying blood biochemical parameters of the volunteers. The survival of the bacteria was assessed using PCR-based detection of P. acidilactici MTCC5101 in fecal samples. The probiotic nature of P. acidilactici MTCC 5101 was strengthened by its adherence to the intestinal epithelial Caco-2 cell line in the in vitro SEM observations. Oral feeding study for assessing the survival of bacteria in GI tract of volunteers showed the strain to be established in the GI tract which survived for about 2 weeks after feeding.
authors.affiliation: Department of Biotechnology, Punjabi University, Punjab, Patiala 147 002, India.
authors.forename: Praveen P
authors.initials: PP
authors.lastname: Balgir
authors.forename: Baljinder
authors.initials: B
authors.lastname: Kaur
authors.forename: Tejinder
authors.initials: T
authors.lastname: Kaur
authors.forename: Natisha
authors.initials: N
authors.lastname: Daroch
authors.forename: Gurpreet
authors.initials: G
authors.lastname: Kaur
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D018938:Caco-2 Cells; D020895:Enterocytes / Q000166:cytology / Q000382:microbiology*; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000382:microbiology*; D008297:Male; D050296:Microbial Viability; D010376:Pediococcus / Q000166:cytology / Q000502:physiology*; D019936:Probiotics; D013997:Time Factors