title: Poly(ethylene glycol) grafting to poly(ether imide) membranes: influence on protein adsorption and thrombocyte adhesion.
abstract: The chain length and end groups of linear PEG grafted on smooth surfaces is known to influence protein adsorption and thrombocyte adhesion. Here, it is explored whether established structure function relationships can be transferred to application relevant, rough surfaces. Functionalization of poly(ether imide) (PEI) membranes by grafting with monoamino PEG of different chain lengths (Mn  =1 kDa or 10 kDa) and end groups (methoxy or hydroxyl) is proven by spectroscopy, changes of surface hydrophilicity, and surface shielding effects. The surface functionalization does lead to reduction of adsorption of BSA, but not of fibrinogen. The thrombocyte adhesion is increased compared to untreated PEI surfaces. Conclusively, rough instead of smooth polymer or gold surfaces should be investigated as relevant models.
authors.affiliation: Institute of Biomaterial Science and Berlin-Brandenburg Centre for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Kantstr. 55, 14513, Teltow, Germany; Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Straße 24-25, 14476 Potsdam, Germany; Helmholtz Virtual Institute - Multifunctional Biomaterials for Medicine, Kantstr. 55, 14513, Teltow, Germany.
authors.forename: Axel T
authors.initials: AT
authors.lastname: Neffe
authors.forename: Maik
authors.initials: M
authors.lastname: von Ruesten-Lange
authors.forename: Steffen
authors.initials: S
authors.lastname: Braune
authors.forename: Karola
authors.initials: K
authors.lastname: Luetzow
authors.forename: Toralf
authors.initials: T
authors.lastname: Roch
authors.forename: Klaus
authors.initials: K
authors.lastname: Richau
authors.forename: Friedrich
authors.initials: F
authors.lastname: Jung
authors.forename: Andreas
authors.initials: A
authors.lastname: Lendlein
mesh_terms: D000327:Adsorption; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects*; D002478:Cells, Cultured; D005340:Fibrinogen / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D010973:Platelet Adhesiveness / Q000187:drug effects*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D008567:Membranes, Artificial; D011108:Polymers; C433673:polyetherimide; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D005340:Fibrinogen
keywords: biomaterials; poly(ethylene glycol); protein adsorption; surface functionalization; thrombocyte adhesion