title: Imaging HIV-1 nuclear pre-integration complexes.
abstract: Advancements in fluorescent microscopy techniques now permit investigation of HIV-1 biology exploiting tools alternative to conventional molecular biology. Here we describe a novel, fluorescence-based method to visualize HIV-1 viral particles within intact nuclei of infected cells. This method allows investigating the localization of pre-integration complexes within the nuclear compartment with respect to the nuclear envelope and the chromatin territories.
authors.affiliation: Centre for Integrative Biology (CIBIO), Mattarello, Trento, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Cereseto
authors.forename: Mauro
authors.initials: M
authors.lastname: Giacca
mesh_terms: D002467:Cell Nucleus / Q000821:virology*; D005455:Fluorescent Antibody Technique; D049452:Green Fluorescent Proteins / Q000235:genetics; D057809:HEK293 Cells; D019427:HIV Integrase / Q000235:genetics; D015497:HIV-1 / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D016662:Virus Integration
chemical_list: D049452:Green Fluorescent Proteins; D019427:HIV Integrase