title: Substrate phage display for protease substrate sequence characterization: bovine factor Xa as a model system.
abstract: Regulatory proteases modulate proteomic dynamics with a spectrum of specificities against substrate proteins. Substrate phage display is one of the key methodologies in producing substrate sequence information in vitro. Factor Xa, a key regulatory protease in the blood coagulation system, is used as a model system to demonstrate a high-throughput procedure to quantitatively characterize substrate sequences and their susceptibilities for enzymatic cleavage. This methodology can be generalized to proteases for which the active forms (not necessarily purified forms) are available for the in vitro experiments.
authors.affiliation: Genomics Research Center, Academia Sinica, Taipei, Taiwan.
authors.forename: Hung-Ju
authors.initials: HJ
authors.lastname: Hsu
authors.forename: An-Suei
authors.initials: AS
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D004247:DNA / Q000302:isolation & purification; D015951:Factor Xa / Q000378:metabolism*; D008954:Models, Biological; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D020539:Sequence Analysis, Protein; D013379:Substrate Specificity
chemical_list: D019151:Peptide Library; D010455:Peptides; D004247:DNA; D015951:Factor Xa