title: Differential expression of cysteine peptidase genes in the inner integument and endosperm of developing seeds of Jatropha curcas L. (Euphorbiaceae).
abstract: In several plant tissues, programmed cell death (PCD) is mediated by the combined action of cysteine peptidases, namely KDEL-tailed cysteine peptidases (KDEL-CysEP) and vacuolar processing enzymes (VPE). Here, we performed a search of the draft genome of Jatropha curcas L. (Euphorbiaceae) and identified 2 genes for KDEL-CysEP (Jc-CysEP1 and Jc-CysEP2) and 3 genes for VPE (Jc-βVPE, Jc-γVPE and Jc-δVPE) and determined the expression patterns of these genes by RT-qPCR in integument and cellular endosperm of seeds collected at seven different developmental stages. We were able to demonstrate that the expression of Jc-CysEP1, Jc-CysEP2, Jc-βVPE and Jc-γVPE proceeded rapidly from Stage IV, with Jc-CysEP2 displaying the highest relative expression; expression of Jc-δVPE could not be detected in any of the tissues/developmental stages analyzed. Additionally, we showed that the expression pattern of these peptidases correlates with anatomical changes in integument and cellular endosperm, thus suggesting a role for both classes of peptidases in PCD and in protein processing, both of which occur simultaneously in each of these tissues.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, Brazil.
authors.forename: Antônio J
authors.initials: AJ
authors.lastname: Rocha
authors.forename: Emanoella L
authors.initials: EL
authors.lastname: Soares
authors.forename: José H
authors.initials: JH
authors.lastname: Costa
authors.forename: Washington L G
authors.initials: WL
authors.lastname: Costa
authors.forename: Arlete A
authors.initials: AA
authors.lastname: Soares
authors.forename: Fábio C S
authors.initials: FC
authors.lastname: Nogueira
authors.forename: Gilberto B
authors.initials: GB
authors.lastname: Domont
authors.forename: Francisco A P
authors.initials: FA
authors.lastname: Campos
mesh_terms: D017209:Apoptosis; D003545:Cysteine / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D056625:Endosperm / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant / Q000235:genetics*; D023281:Genomics; D031286:Jatropha / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D009842:Oligopeptides; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D021382:Protein Sorting Signals; D012639:Seeds / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000502:physiology
chemical_list: D017931:DNA Primers; D009842:Oligopeptides; D010940:Plant Proteins; D021382:Protein Sorting Signals; C073762:lysyl-aspartyl-glutamyl-leucine; D003546:Cysteine Endopeptidases; C089278:vacuolar processing enzyme; D003545:Cysteine
keywords: BLAST; EST; KDEL-CysEP; KDEL-tailed cysteine peptidase; Oilseeds; Programmed cell death; Protein processing; SRA; TSA; VPE; Vacuolar processing enzyme; WGS; basic local alignment search tool; cDNA; complementary DNA; expressed sequence tag; non redundant; nr; sequence read archive; transcriptome shotgun assembly; whole-genome shotgun