title: Infection of mouse bone marrow-derived immature dendritic cells with classical swine fever virus C-strain promotes cells maturation and lymphocyte proliferation.
abstract: In this study, the interactions of classical swine fever virus (CSFV) C-strain and the virulent GSLZ strain with mouse bone marrow-derived immature dendritic cells (BM-imDCs) were investigated for the first time. Both the C-strain and the virulent GSLZ strain could effectively infect and replicate in mouse BM-imDCs. C-strain-infected BM-imDCs showed a greatly enhanced degree of maturation, and could effectively promote the expansion and proliferation of allogeneic naive T cells. The C-strain induced a stronger Th1 response. Infection with the virulent GSLZ strain had no obvious influence on cell maturation or lymphocyte proliferation, and failed to induce any obvious immune response. The results of this study provided initial information for research of the immunologic mechanisms of CSFV using mouse DCs as the model cells.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 1 Xujiaping, Lanzhou, Gansu 730046, China.
authors.forename: Fu-Ying
authors.initials: FY
authors.lastname: Zheng
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Qiu
authors.forename: Huai-Jie
authors.initials: HJ
authors.lastname: Jia
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Chen
authors.forename: Shuang
authors.initials: S
authors.lastname: Zeng
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: He
authors.forename: Yong-Xiang
authors.initials: YX
authors.lastname: Fang
authors.forename: Guo-Zhen
authors.initials: GZ
authors.lastname: Lin
authors.forename: Zhi-Zhong
authors.initials: ZZ
authors.lastname: Jing
mesh_terms: D000818:Animals; D002472:Cell Transformation, Viral / Q000502:physiology*; D006691:Classical Swine Fever / Q000276:immunology / Q000503:physiopathology*; D006692:Classical Swine Fever Virus; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D008213:Lymphocyte Activation / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D013552:Swine
chemical_list: D016207:Cytokines
keywords: Classical swine fever virus C-strain; Classical swine fever virus GSLZ strain; Cytokine; Dendritic cells; Maturation; Stimulation index