title: Protective activity of Panduratin A against thioacetamide-induced oxidative damage: demonstration with in vitro experiments using WRL-68 liver cell line.
abstract: BACKGROUND
  Chalcone Panduratin A (PA) has been known for its antioxidant property, but its merits against oxidative damage in liver cells has yet to be investigated. Hence, the paper aimed at accomplishing this task with normal embryonic cell line WRL-68.
  
  
  METHODS
  PA was isolated from Boesenbergia rotunda rhizomes and its 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and ferric reducing power (FRAP) activities were measured in comparison with that of the standard reference drug Silymarin (SI). Oxidative damage was induced by treating the cells with 0.04 g/ml of toxic thioacetamide for 60 minutes followed by treatment with 1, 10 and 100 μg/ml concentrations of either PA or SI. The severities of oxidative stress in the control and experimental groups of cells were measured by Malondialdehyde (MDA) levels, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities.
  
  
  RESULTS
  PA exhibited an acceptable DPPH scavenging and FRAP activities close to that of Silymarin. Treating the injured cells with PA significantly reduced the MDA level and increased the cell viability, comparable to SI. The activities of SOD, CAT and GPx were significantly elevated in the PA-treated cells in a dose dependent manner and again similar to SI.
  
  
  CONCLUSIONS
  Collectively, data suggested that PA has capacity to protect normal liver cells from oxidative damage, most likely via its antioxidant scavenging ability.
authors.forename: Suzy M
authors.initials: SM
authors.lastname: Salama
authors.forename: Ahmed S
authors.initials: AS
authors.lastname: AlRashdi
authors.forename: Mahmood A
authors.initials: MA
authors.lastname: Abdulla
authors.forename: Pouya
authors.initials: P
authors.lastname: Hassandarvish
authors.affiliation: Biophysics Department, Faculty of Medicine, Erciyes University, 38039, Kayseri, Turkey. mehmet.bilgen@yahoo.com.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Bilgen
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001713:Biphenyl Compounds; D002374:Catalase / Q000378:metabolism; D002460:Cell Line; D047188:Chalcones / Q000737:chemistry / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013853:Thioacetamide / Q000009:adverse effects; D030521:Zingiberaceae / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D047188:Chalcones; D010851:Picrates; D010936:Plant Extracts; D020011:Protective Agents; D013853:Thioacetamide; C482884:panduratin A; D008315:Malondialdehyde; C004931:1,1-diphenyl-2-picrylhydrazyl; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase