title: Expression and localization of TRPC proteins in rat ventricular myocytes at various developmental stages.
abstract: Growing evidence indicates that transient receptor potential canonical (TRPC) channels play important roles in various Ca(2+)-mediated physiological and pathophysiological processes, including development. Many types of TRPC proteins are expressed in the heart. However, limited data are available comparing the expression and localization among TRPC proteins in the ventricular myocyte at various developmental stages. Our purpose is to investigate the expression and localization profile of TRPC proteins in ventricular myocytes of fetal (18.5 days), neonatal (< 24 h after birth) and adult (8 week old) rats. Western blotting, immunofluorescence and confocal laser scanning microscopy were employed. TRPC1/3-6 proteins were expressed in the rat ventricle throughout the three developmental stages. The expression profile of TRPC1/3/4 in the ventricle followed an upward trend from the fetus to the adult. By contrast, TRPC6 in the ventricle was expressed at the highest level in the fetal group and was sharply down-regulated immediately after birth. TRPC5 expression in the ventricle did not change significantly during the three stages. TRPC1/3/5/6 proteins were localized to the T-tubule and TRPC1/3/4/6 to intercalated disks in adult myocytes. The wide spatiotemporal overlap and dynamic regulation of TRPC expression in ventricular myocytes indicates potential complex combinations and redundancy of native TRPC proteins in the heart and gives important clues for further investigations into the exact subunit compositions and functional properties of native TRPC channels in the heart.
authors.affiliation: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, 100069, Beijing, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jiang
authors.forename: Haixia
authors.initials: H
authors.lastname: Huang
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhao
authors.forename: Ying
authors.initials: Y
authors.lastname: Feng
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Weizhen
authors.initials: W
authors.lastname: Niu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005455:Fluorescent Antibody Technique; D006352:Heart Ventricles / Q000166:cytology* / Q000254:growth & development*; D008297:Male; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D050052:TRPC Cation Channels / Q000032:analysis*
chemical_list: D050052:TRPC Cation Channels