title: Increased RCAS1 expression is associated with advanced histopathological stage and poor prognosis in patients with gastric adenocarcinoma.
abstract: BACKGROUND
  The receptor-binding cancer antigen expressed on SiSo cells (RCAS1) is a human tumor-associated antigen that has been considered to play a crucial role in tumor progression by enabling cancer cells to evade immune surveillance. The present study aimed to evaluate the clinical significance of the RCAS1 expression in gastric adenocarcinoma.
  
  
  METHODS
  RCAS1 protein expression was assessed immunohistochemically on 54 gastric adenocarcinoma tissue samples and was analyzed in relation to clinicopathological parameters, tumor proliferative capacity, and patients' survival.
  
  
  RESULTS
  Enhanced RCAS1 expression levels were significantly associated with advanced histopathological stage and presence of organ metastasis (P = 0.0084 and P = 0.0327). Gastric cancer patients with elevated RCAS1 expression levels showed significantly shorter survival times compared to those with low RCAS1 expression (log-rank test, P = 0.0168). In multivariate analysis, histopathological stage and grade of differentiation as well as the RCAS1 expression were identified as independent prognostic factors (Cox regression analysis, P = 0.0204, P = 0.0035, and P = 0.0081).
  
  
  CONCLUSIONS
  Our data support the evidence that RCAS1 upregulation may contribute to gastric malignant progression, representing a useful biomarker to predict the biological behaviour and prognosis in gastric neoplasia.
authors.affiliation: First Department of Pathology, Medical School, National and Kapodistrian University of Athens, 75 M. Asias steet, Goudi, GR11527 Athens, Greece ; Department of Food Science and Nutrition, University of the Aegean, Myrina, Lemnos, Greece.
authors.forename: Constantinos
authors.initials: C
authors.lastname: Giaginis
authors.forename: Themistoclis
authors.initials: T
authors.lastname: Efkarpidis
authors.forename: Paraskevi
authors.initials: P
authors.lastname: Alexandrou
authors.forename: Efstratios
authors.initials: E
authors.lastname: Patsouris
authors.forename: Gregory
authors.initials: G
authors.lastname: Kouraklis
authors.forename: Stamatios
authors.initials: S
authors.lastname: Theocharis
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C120762:EBAG9 protein, human; D012333:RNA, Messenger