title: High prevalence of HCMV and viral load in tumor tissues and peripheral blood of glioblastoma multiforme patients.
abstract: Glioblastoma multiforme is the most prevalent and malignant tumor of the central nervous system. In the last few years, accumulating evidence has suggested an association between human cytomegalovirus (HCMV) infection and glioblastoma multiforme. In this study, tumor tissues and peripheral blood of patients with glioblastoma multiforme were examined for the presence of HCMV DNA. Twenty-two fresh surgical brain specimens and 20 peripheral blood samples were analyzed by real-time PCR (qPCR) and hemi-nested PCR (nPCR) for the presence of pp65 and (glycoprotein B) gB viral genomic regions, respectively. HCMV DNA was detected in the majority of the tumor samples analyzed (95% by qPCR and 91% by nPCR). About half of the patients with tumors positive for HCMV also had detectable viral DNA in their peripheral blood (47% by qPCR and 61% by nPCR). Genome copy numbers were determined and in the majority of the tumor samples cellular DNA outnumbers viral DNA (average of 1 infected cell in 33 cells). The gB genotypes were determined in HCMV-positive samples and gB2 was the most prevalent genotype in the tumor and blood samples. The results show a high prevalence of HCMV in glioblastoma multiforme samples reinforcing a possible association between HCMV infection and tumor development.
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), Santo André, SP, Brazil.
authors.forename: Claudia Januário
authors.initials: CJ
authors.lastname: dos Santos
authors.forename: Lucas Matheus
authors.initials: LM
authors.lastname: Stangherlin
authors.forename: Eberval Gadelha
authors.initials: EG
authors.lastname: Figueiredo
authors.forename: Clemar
authors.initials: C
authors.lastname: Corrêa
authors.forename: Manoel Jacobsen
authors.initials: MJ
authors.lastname: Teixeira
authors.forename: Maria Cristina Carlan
authors.initials: MC
authors.lastname: da Silva
mesh_terms: D001769:Blood / Q000821:virology*; D001921:Brain / Q000821:virology*; D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000453:epidemiology* / Q000821:virology*; D004279:DNA, Viral / Q000302:isolation & purification; D005909:Glioblastoma / Q000150:complications*; D006801:Humans; D016133:Polymerase Chain Reaction; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D019562:Viral Load
chemical_list: D004279:DNA, Viral
keywords: detection; glioblastoma multiforme; human cytomegalovirus