title: Human beta papillomavirus DNA study in primary cutaneous squamous cell carcinomas and their corresponding metastases.
abstract: The association between beta human papillomavirus (HPV) types and cutaneous squamous cell carcinomas (cSCCs) is controversial. Several studies have found such an association, especially at early stages of carcinogenesis, but the presence of beta HPV types in aggressive cSCCs has only been reported in three patients previously. We aimed to search for beta HPV DNA in primary cSCCs and their corresponding lymph node metastases in a series of patients. The presence of DNA from 25 beta HPV types was determined using a multiplex PCR protocol in 35 primary cSCCs from 35 patients and their corresponding lymph node metastases. DNA from beta HPV types was detected in 9 % of primary cSCCs and in 13 % of metastases. No primary cutaneous SCC or lymphatic metastases were found to share the same HPV DNA. These data suggest that beta HPV types do not play an etiopathogenic role in advanced stages of squamous cell carcinogenesis.
authors.affiliation: Department of Dermatology, Hospital del Mar, Parc de Salut Mar, Pg/Marítim 25-29, 08003, Barcelona, Spain, atoll@parcdesalutmar.cat.
authors.forename: A
authors.initials: A
authors.lastname: Toll
authors.forename: B
authors.initials: B
authors.lastname: Lloveras
authors.forename: E
authors.initials: E
authors.lastname: Masferrer
authors.forename: C
authors.initials: C
authors.lastname: Ferrándiz-Pulido
authors.forename: V
authors.initials: V
authors.lastname: García-Patos
authors.forename: T
authors.initials: T
authors.lastname: Gheit
authors.forename: R M
authors.initials: RM
authors.lastname: Pujol
authors.forename: M
authors.initials: M
authors.lastname: Tommasino
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D052686:Betapapillomavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000821:virology; D004279:DNA, Viral; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000235:genetics* / Q000821:virology; D012878:Skin Neoplasms / Q000235:genetics* / Q000821:virology
chemical_list: D004279:DNA, Viral