title: 7T mMR in the assessment of acute arterial mesenteric ischemia in a rat model.
abstract: To validate a rat model of acute arterial mesenteric ischemia correlating MRI patterns with macro and microscopic changes in the small bowel. Thirty Sprague-Dawley rats were assigned to two experimental groups (Group I and Group II) of fifteen rats each. Group I underwent surgical procedure of superior mesenteric artery (SMA) ligation, followed by macroscopic observation. In Group II, a loop was tied loosely around the SMA without occluding the vessel. Three days after surgery, the loop was tied by external tips to completely occlude the artery. 7T microMR (7Tesla microMR) was performed before and 8 hours after SMA occlusion. At predetermined time-points the histopathological examinations were performed in both of groups. Macroscopic monitoring revealed thinning of mesenteric vessels, hypotonic reflex ileus and chromatic change of some loops. 7T microMR sequences evidenced loop dilation with gas-fluid mixed stasis, intraperitoneal free fluid and bowel wall hyperintensity. There were no significant differences in the histological analysis between the two groups. The gap of three days from surgery, adopted in the Group 2, allowed to avoid signs of peritoneal and mesenteric irritation which could bias imaging patterns. MR succeeded to identify the signs of arterial mesenteric ischemia.
authors.affiliation: Institute of Radiology, Second University of Naples, Naples, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Berritto
authors.forename: F
authors.initials: F
authors.lastname: Iacobellis
authors.forename: F
authors.initials: F
authors.lastname: Somma
authors.forename: M
authors.initials: M
authors.lastname: Corona
authors.forename: A
authors.initials: A
authors.lastname: Faggian
authors.forename: A
authors.initials: A
authors.lastname: Iacomino
authors.forename: B
authors.initials: B
authors.lastname: Feragalli
authors.forename: L
authors.initials: L
authors.lastname: Saba
authors.forename: M
authors.initials: M
authors.lastname: La Porta
authors.forename: R
authors.initials: R
authors.lastname: Grassi
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007511:Ischemia / Q000175:diagnosis*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D017538:Mesenteric Artery, Superior / Q000473:pathology*; D065666:Mesenteric Ischemia; D051381:Rats; D017207:Rats, Sprague-Dawley; D014652:Vascular Diseases / Q000175:diagnosis*