title: Changes of bacterial and fungal community compositions during vermicomposting of vegetable wastes by Eisenia foetida.
abstract: Changes of bacterial and fungal community during vermicomposting of vegetable wastes by hatchling, juvenile and adult Eisenia foetida were investigated through analysis of the extracted bacterial 16S rDNA and fungal 18S rDNA with quantitative polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and sequencing. After 60days of composting, significantly lower values of microbial activity and bacterial and fungal densities were revealed in the products of composting with earthworms than in the control (without earthworms). PCR-DGGE images showed vermicomposting significantly enhanced the diversities of bacterial and fungal communities. However, for their structures, sequencing results revealed that, compared to the control where the bacterial Firmicutes were predominant, in the composts with earthworms, the bacterial Bacteroidetes and Actinomycetes, and the fungal Sordariomycetes were found dominant. In addition, some beneficial species of bacteria and fungi against pathogens were also isolated from the vermicomposting products.
authors.affiliation: Graduate School of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Kui
authors.initials: K
authors.lastname: Huang
authors.forename: Fusheng
authors.initials: F
authors.lastname: Li
authors.forename: Yongfen
authors.initials: Y
authors.lastname: Wei
authors.forename: Xuemin
authors.initials: X
authors.lastname: Chen
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Fu
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000254:growth & development*; D001673:Biodegradation, Environmental; D044822:Biodiversity; D058645:Denaturing Gradient Gel Electrophoresis; D005452:Fluoresceins / Q000378:metabolism; D005658:Fungi / Q000235:genetics / Q000254:growth & development*; D006868:Hydrolysis; D009835:Oligochaeta / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil; D014675:Vegetables / Q000378:metabolism*; D014866:Waste Products / Q000032:analysis*
chemical_list: D005452:Fluoresceins; D012336:RNA, Ribosomal, 16S; D012987:Soil; D014866:Waste Products; C018506:diacetylfluorescein
keywords: Bacteria; Fungi; PCR–DGGE; Vegetable wastes; Vermicomposting