title: DNA barcoding for the identification of eight species members of the Thai Hyrcanus Group and investigation of their stenogamous behavior.
abstract: Eight species members of the Thai Hyrcanus Group were identified based on the intact morphology and molecular analysis (COI barcoding, 658 bp) of F1-progenies. Five iso-female lines of each species were pooled in order to establish stock colonies. A stenogamous colony of each species was investigated by making 200 and 300 newly emerged adult females and males co-habit in a 30 cm cubic cage for one week. After ovipositon, the spermathecae of females were examined for sperms. The results revealed that Anopheles argyropus, Anopheles crawfordi, Anopheles nitidus, Anopheles pursati, Anopheles sinensis, Anopheles nigerrimus, Anopheles paraliae and Anopheles peditaeniatus yielded insemination rates of 0%, 0%, 0%, 31%, 33%, 42%, 50% and 77%, respectively. Continuous selection to establish stenogamous colonies indicated that An. sinensis, An. pursati, An. nigerrimus, An. paraliae and An. peditaeniatus provided insemination rates of 33-34%, 27-31%, 42-58%, 43-57% and 61-86% in 1, 2, 5, 6 and 20 generations of passages, respectively.
authors.affiliation: Office of Disease Prevention and Control, 10th, Department of Disease Control, Ministry of Public Health, Chiang Mai 50100, Thailand.
authors.forename: Adulsak
authors.initials: A
authors.lastname: Wijit
authors.forename: Atiporn
authors.initials: A
authors.lastname: Saeung
authors.forename: Visut
authors.initials: V
authors.lastname: Baimai
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Otsuka
authors.forename: Sorawat
authors.initials: S
authors.lastname: Thongsahuan
authors.forename: Kritsana
authors.initials: K
authors.lastname: Taai
authors.forename: Wichai
authors.initials: W
authors.lastname: Srisuka
authors.forename: Siripan
authors.initials: S
authors.lastname: Songsawatkiat
authors.forename: Sriwatapron
authors.initials: S
authors.lastname: Sor-suwan
authors.forename: Chayanit
authors.initials: C
authors.lastname: Hempolchom
authors.forename: Pradya
authors.initials: P
authors.lastname: Somboon
authors.forename: Wej
authors.initials: W
authors.lastname: Choochote
mesh_terms: D000818:Animals; D000852:Anopheles / Q000145:classification* / Q000235:genetics / Q000502:physiology; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D005260:Female; D017344:Genes, Insect; D005819:Genetic Markers; D014644:Genetic Variation; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D012726:Sexual Behavior, Animal; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA
keywords: Anopheles; DNA barcodes; Eurygamy; Hyrcanus Group; Stenogamy