title: Long-range organization of a satellite DNA family flanking the beet cyst nematode resistance locus (Hs1) on chromosome-1 of B. patellaris and B. procumbens.
abstract: New members of a satellite DNA family (Sat 121), specific for wild beets of the section Procumbentes of the genus Beta, were isolated. Sequence analysis showed that the members of Sat-121 fall into two distinct classes. The organization of Sat-121 in the vicinity of a beet cyst nematode (Heterodera schachtii Schm.) resistance locus (Hs1) in B. patellaris and B. procumbens was investigated by pulsed-field gel electrophoresis (PFGE) using DNA from a series of resistant monosomic fragment additions, each containing an extra chromosome fragment of B. patellaris chromosome-1 (pat-1) in B. vulgaris. In this way several clusters of Sat-121 flanking the Hs1 (pat-1) locus were identified. In nematode resistant diploid introgressions (2n=18), which contain small segments of B. procumbens chromosome-1 (pro-1) in B. vulgaris, only two major Sat-121 clusters were detected near the Hs1 (pro-1) locus.
authors.affiliation: Department of Molecular Biology, DLO - Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O.Box 16, NL-6700 AA, Wageningen, The Netherlands.
authors.forename: E M
authors.initials: EM
authors.lastname: Salentijn
authors.forename: N N
authors.initials: NN
authors.lastname: Sandal
authors.forename: R
authors.initials: R
authors.lastname: Klein-Lankhorst
authors.forename: W
authors.initials: W
authors.lastname: Lange
authors.forename: T S
authors.initials: TS
authors.lastname: De Bock
authors.forename: K A
authors.initials: KA
authors.lastname: Marcker
authors.forename: W J
authors.initials: WJ
authors.lastname: Stiekema