title: Protein adsorption on various plasma-treated polyethylene terephthalate substrates.
abstract: Protein adhesion and cell response to plasma-treated polymer surfaces were studied. The polymer polyethylene terephthalate (PET) was treated in either an oxygen plasma to make the surface hydrophilic, or a tetrafluoromethane CF(4) plasma to make the surface hydrophobic. The plasma source was radiofrequency (RF) discharge. The adsorption of albumin and other proteins from a cell-culture medium onto these surfaces was studied using a quartz crystal microbalance (QCM), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The cellular response to plasma-treated surfaces was studied as well using an MTT assay and scanning electron microscopy (SEM). The fastest adsorption rate was found on the hydrophilic oxygen plasma-treated sample, and the lowest was found on the pristine untreated sample. Additionally, the amount of adsorbed proteins was higher for the oxygen-plasma-treated surface, and the adsorbed layer was more viscoelastic. In addition, cell adhesion studies support this finding because the best cell adhesion was observed on oxygen-plasma-treated substrates.
authors.affiliation: Jozef Stefan International Postgraduate School, Jamova 39, Ljubljana 1000, Slovenia. alenka.vesel@guest.arnes.si.
authors.forename: Nina
authors.initials: N
authors.lastname: Recek
authors.forename: Morana
authors.initials: M
authors.lastname: Jaganjac
authors.forename: Metod
authors.initials: M
authors.lastname: Kolar
authors.forename: Lidija
authors.initials: L
authors.lastname: Milkovic
authors.forename: Miran
authors.initials: M
authors.lastname: Mozetič
authors.forename: Karin
authors.initials: K
authors.lastname: Stana-Kleinschek
authors.forename: Alenka
authors.initials: A
authors.lastname: Vesel
mesh_terms: D000327:Adsorption; D000418:Albumins / Q000737:chemistry; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D048430:Cell Shape; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D055767:Immobilized Proteins / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D010100:Oxygen / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D058626:Plasma Gases / Q000737:chemistry; D011093:Polyethylene Terephthalates / Q000737:chemistry*; D058525:Quartz Crystal Microbalance Techniques; D013499:Surface Properties
chemical_list: D000418:Albumins; D020099:Coated Materials, Biocompatible; D055767:Immobilized Proteins; D058626:Plasma Gases; D011093:Polyethylene Terephthalates; D010100:Oxygen