title: Some genetical and biochemical attempts to elucidate the energetics of sugar uptake and explain the Kluyver effect in the yeast Kluyveromyces lactis.
abstract: A mutant of Kluyveromyces lactis is described which did not grow with substrates giving the Kluyver effect. In addition it could not grow with non-fermentable carbon sources, although it was not respiratory deficient. Abolition of respiration by cyanide also caused inability to grow with substrates showing the Kluyver effect in the wild-type strain. When the yeasts were using substrates showing the Kluyver effect, shifting to anaerobic conditions gave an immediate decrease in the intracellular concentration of D-glucose 6-phosphate. The results obtained were consistent with the need of a common respiratory and/or anabolic pathway for the utilization of these substrates.
authors.affiliation: Physiologisch-chemisches Institut, Universität Tübingen, D-7400, Tübingen, Germany.
authors.forename: K D
authors.initials: KD
authors.lastname: Entiani
authors.forename: J A
authors.initials: JA
authors.lastname: Barnett