title: [Phenotypic screens or one stone to kill two birds: discover the target and its pharmacological regulator].
abstract: Phenotypic screens, in which chemical libraries are assayed on cells with the aim to isolate compounds that interfere with a given cell function, are a risky but powerful strategy to discover, in the same approach, new therapeutic targets and the compounds able to regulate them. With a strong experience of nearly 10 years in the field, we present the advantages of such an approach, the possible troubles and technical solutions. We also present in this paper a french network which has been recently built and that gather all the competencies needed for screening approaches.
authors.affiliation: Institut Albert Bonniot, CRI Inserm/Université Joseph Fourier (UJF) U823, équipe 3 Polarité, développement et cancer, rond-point de la Chantourne, 38706 La Tronche Cedex, France.
authors.forename: Renaud
authors.initials: R
authors.lastname: Prudent
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Soleilhac
authors.forename: Caroline
authors.initials: C
authors.lastname: Barette
authors.forename: Marie-Odile
authors.initials: MO
authors.lastname: Fauvarque
authors.forename: Laurence
authors.initials: L
authors.lastname: Lafanechère
mesh_terms: D000465:Algorithms; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods*; D005602:France; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods; D006801:Humans; D058990:Molecular Targeted Therapy / Q000379:methods*; D010641:Phenotype; D054852:Small Molecule Libraries / Q000032:analysis* / Q000494:pharmacology* / Q000600:supply & distribution
chemical_list: D054852:Small Molecule Libraries