title: Neuroprotective Role of a Novel Copper Chelator against Aβ 42 Induced Neurotoxicity.
abstract: Alzheimer's disease (AD) is a progressive neurodegenerative disease and associated with the extracellular deposits of amyloid- β peptide in hippocampus region. Metal ions like Cu, Fe and Zn are known to associate with the amyloid beta (A β ) at high concentration and interaction of these ions with soluble and aggregated forms of A β peptide help in development of AD. Here we showed Cu mediated neurotoxicity in the eye tissues of transgenic Drosophila expressing human amyloid β and its rescue through a novel Cu chelator. In this context, we have synthesised and characterized the compound L 2,6-Pyridinedicarboxylic acid, 2,6-bis[2-[(4-carboxyphenyl) methylene] hydrazide] by Mass spectra (MS) and Elemental analysis (EA). The Cu chelation potential of the compound L is tested in vivo in Drosophila. Oral administration of Copper to the transgenic larvae resulted in severe degeneration in eye tissues, which was rescued by the supplementation of compound L. The levels of anti-oxidant markers like SOD and MDA were measured in compound L treated flies and found a significant rescue (P < 0.001). Further rescue of the eye degeneration phenotypes as revealed by SEM affirm the role of copper in A β toxicity. Hence, use of compound L, an amidoamine derivative, could be a possible therapeutic measure for A β induced neurotoxicity.
authors.affiliation: Cell and Neurobiology Laboratory, Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanas 221005, India.
authors.forename: Sandeep Kumar
authors.initials: SK
authors.lastname: Singh
authors.forename: Priti
authors.initials: P
authors.lastname: Sinha
authors.forename: L
authors.initials: L
authors.lastname: Mishra
authors.forename: S
authors.initials: S
authors.lastname: Srikrishna