title: Evaluation of laser speckle contrast imaging as an intrinsic method to monitor blood brain barrier integrity.
abstract: The integrity of the blood brain barrier (BBB) can contribute to the development of many brain disorders. We evaluate laser speckle contrast imaging (LSCI) as an intrinsic modality for monitoring BBB disruptions through simultaneous fluorescence and LSCI with vertical cavity surface emitting lasers (VCSELs). We demonstrated that drug-induced BBB opening was associated with a relative change of the arterial and venous blood velocities. Cross-sectional flow velocity ratio (veins/arteries) decreased significantly in rats treated with BBB-opening drugs, ≤0.81 of initial values.
authors.affiliation: The Edward S. Rogers Sr. Department of Electrical and Computer Engineering, University of Toronto, 10 King's College Road, Toronto, ON M5S 3G4, Canada ; Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON M5S 3G9, Canada.
authors.forename: Suzie
authors.initials: S
authors.lastname: Dufour
authors.forename: Yaaseen
authors.initials: Y
authors.lastname: Atchia
authors.forename: Raanan
authors.initials: R
authors.lastname: Gad
authors.forename: Dene
authors.initials: D
authors.lastname: Ringuette
authors.forename: Iliya
authors.initials: I
authors.lastname: Sigal
authors.forename: Ofer
authors.initials: O
authors.lastname: Levi
keywords: (140.2020) Diode lasers; (170.0110) Imaging systems; (170.3880) Medical and biological imaging; (170.6480) Spectroscopy, speckle