title: Methyl-combing: single-molecule analysis of DNA methylation on stretched DNA fibers.
abstract: The methyl-combing technique combines the dynamic molecular combing method with the detection of DNA modifications. The assay allows the single-molecule analysis of epigenetic marks on regularly stretched DNA fibers, at the megabase scale with kilobase resolution. The protocol presented in this chapter is based on proof-of-principle experiments where the single-molecule detection of DNA methylation has been performed on unmethylated and in vitro methylated λ phage DNA.
authors.affiliation: Biochemistry Center Regensburg, University of Regensburg, Regensburg, Germany.
authors.forename: Attila
authors.initials: A
authors.lastname: Németh
mesh_terms: D044503:5-Methylcytosine / Q000276:immunology; D010582:Bacteriophage lambda / Q000378:metabolism; D019175:DNA Methylation; D015342:DNA Probes / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008967:Molecular Biology / Q000379:methods*; D009693:Nucleic Acid Hybridization
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D044503:5-Methylcytosine