title: Impact of self-assembled monolayer films with specific chemical group on bFGF adsorption and endothelial cell growth on gold surface.
abstract: In this study, thiols ended with methyl, carboxyl, hydroxy, and amino groups are self-assembled on gold surfaces. The X-ray photoelectron spectroscopy test results show that chemical components on the self-assembled surface are similar to those in the theoretical calculations. The atomic force microscope test results show that the molecule assembled on the surface causes no significant variation in the surface roughness before and after the molecule assembly. The water surface contact angle increases with the increasing hydrophilicity of the end groups of the self-assembled monolayer. The surface zeta potential reveals that -COOH surface has the most electronegativity. The resulting substrates are then made to adsorb base fibroblast growth factor (bFGF). The quartz crystal microbalance test results show that the amounts of bFGF adsorbed onto different self-assembled surfaces are -COOH≈-OH>-CH₃ >-NH₂. According to cell culture experiments, endothelial cells have different morphologies after adhering to different surfaces. Furthermore, endothelial cells achieve the quickest proliferation on the -COOH self-assembled surface and the slowest proliferation on the -CH₃ self-assembled surface.
authors.affiliation: Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510640, China; Regenerative Biomaterials Group, National Engineering Research Center for Tissue Reconstruction and Restoration, Guangzhou, 510006, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.forename: Jiahui
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Chen
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry* / Q000494:pharmacology*; D006046:Gold / Q000494:pharmacology*; D018625:Microscopy, Atomic Force; D058525:Quartz Crystal Microbalance Techniques; D055672:Static Electricity; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties; D034421:Sus scrofa; D014867:Water / Q000737:chemistry
chemical_list: D013438:Sulfhydryl Compounds; D014867:Water; D016222:Fibroblast Growth Factor 2; D006046:Gold
keywords: biomaterials; endothelia cell; protein adsorption; self-assemble; thiol