title: HIV-1 reverse transcription.
abstract: Reverse transcription is an obligatory step in retrovirus replication in the course of which the retroviral RNA/DNA-dependent DNA polymerase (RT) copies the single-stranded positive sense RNA genome to synthesize the double-stranded viral DNA. At the same time the RT-associated RNaseH activity degrades the genomic RNA template, which has just been copied. The viral nucleocapsid protein NCp7 is an obligatory partner of RT, chaperoning synthesis of the complete viral DNA flanked by the two long-terminal repeats (LTR), required for viral DNA integration into the host genome and its expression. Here we describe assays for in vitro and ex vivo monitoring of reverse transcription and the chaperoning role of the nucleocapsid protein (NC).
authors.affiliation: Centre International de Recherche en Infectiologie (CIRI)- INSERM U1111 - CNRS UMR5308- Université Lyon 1- ENS de Lyon, Lyon, France.
authors.forename: Andrea
authors.initials: A
authors.lastname: Cimarelli
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Darlix
mesh_terms: D004279:DNA, Viral / Q000096:biosynthesis; D024882:Drug Resistance, Viral; D057809:HEK293 Cells; D054303:HIV Reverse Transcriptase / Q000378:metabolism; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D006801:Humans; D012367:RNA, Viral / Q000235:genetics; D048348:Reverse Transcription; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D054301:gag Gene Products, Human Immunodeficiency Virus; D054303:HIV Reverse Transcriptase