title: Lactational anovulation in mice results from a selective loss of kisspeptin input to GnRH neurons.
abstract: In mammals, lactation is associated with a period of infertility characterized by the loss of pulsatile secretion of GnRH and cessation of ovulatory cycles. Despite the importance of lactational infertility in determining overall fecundity of a species, the mechanisms by which the suckling stimulus suppresses GnRH secretion remain unclear. Because kisspeptin neurons are critical for fertility, the aim of this study was to test the hypothesis that reduced kisspeptin expression might mediate the lactation-induced suppression of fertility, using mouse models. In the rostral periventricular area of the third ventricle (RP3V), a progressive decrease in RP3V Kiss1 mRNA levels was observed during pregnancy culminating in a 10-fold reduction during lactation compared with diestrous controls. This was associated with approximately 60% reduction in the numbers of kisspeptin-immunoreactive neurons in the RP3V detected during lactation. Similarly, in the arcuate nucleus there was also a significant decrease in Kiss1 mRNA levels during late pregnancy and midlactation, and a notable decrease in kisspeptin fiber density during lactation. The functional characteristics of the RP3V kisspeptin input to GnRH neurons were assessed using electrophysiological approaches in an acute brain slice preparation. Although endogenous RP3V kisspeptin neurons were found to activate GnRH neurons in diestrous mice, this was never observed during lactation. This did not result from an absence of kisspeptin receptors because GnRH neurons responded normally to 100 nM exogenous kisspeptin during lactation. The kisspeptin deficit in lactating mice was selective, because GnRH neurons responded normally to RP3V gamma aminobutryic acid inputs during lactation. These data demonstrate that a selective loss of RP3V kisspeptin inputs to GnRH neurons during lactation is the likely mechanism causing lactational anovulation in the mouse.
authors.affiliation: Centre for Neuroendocrinology and Departments of Physiology (X.L., A.E.H.) and Anatomy (R.S.E.B., D.R.G.), School of Medical Sciences, University of Otago, Dunedin, 9054 New Zealand.
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: R S E
authors.initials: RS
authors.lastname: Brown
authors.forename: A E
authors.initials: AE
authors.lastname: Herbison
authors.forename: D R
authors.initials: DR
authors.lastname: Grattan
mesh_terms: D000818:Animals; D000858:Anovulation / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D004594:Electrophysiology; D005260:Female; D005968:Glutamate Decarboxylase / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D059648:Kisspeptins / Q000378:metabolism*; D007774:Lactation / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000378:metabolism*; D010060:Ovulation; D011247:Pregnancy; D020542:Third Ventricle / Q000378:metabolism*
chemical_list: C491407:Kiss1 protein, mouse; D059648:Kisspeptins; D049452:Green Fluorescent Proteins; D007987:Gonadotropin-Releasing Hormone; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1; C401141:glutamate decarboxylase 2