title: The assessment of Proteus mirabilis susceptibility to ceftazidime and ciprofloxacin and the impact of these antibiotics at subinhibitory concentrations on Proteus mirabilis biofilms.
abstract: Rods of the Proteus genus are commonly isolated from patients, especially from the urinary tracts of the catheterised patients. The infections associated with biomaterials are crucial therapeutic obstacles, due to the bactericidal resistance of the biofilm. The aim of this study was to assess the susceptibility of P. mirabilis planktonic forms to ciprofloxacin and ceftazidime, the ability to form biofilm, and the impact of chosen sub-MIC concentrations of these antibiotics on biofilm at different stages of its formation. The research included 50 P. mirabilis strains isolated from wounds and the urinary tracts from patients of the University Hospital No. 1 in Bydgoszcz. The assessment of susceptibility to ciprofloxacin and ceftazidime was conducted using micromethods. The impact of sub-MIC concentrations of the chosen antibiotics on the biofilm was measured using the TTC method. The resistance to ciprofloxacin was confirmed for 20 strains (40.0%) while to ceftazidime for 32 (64.0%) of the tested P. mirabilis strains. All of the tested strains formed biofilm: 24.0% weakly, 26.0% moderately, and 50.0% strongly. It was determined that ciprofloxacin and ceftazidime caused eradication of the biofilm. Moreover, the connection between origin of the strains, biofilm maturity level, and resistance to antibiotics was proved.
authors.affiliation: Department of Microbiology, Faculty of Pharmacy, Nicolaus Copernicus University in Toruń, Collegium Medicum of L. Rydygier in Bydgoszcz, M. Skłodowskiej-Curie 9, 85-094 Bydgoszcz, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Kwiecińska-Piróg
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Skowron
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Zniszczol
authors.forename: Eugenia
authors.initials: E
authors.lastname: Gospodarek
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D055499:Catheter-Related Infections / Q000188:drug therapy* / Q000382:microbiology; D002442:Ceftazidime / Q000008:administration & dosage*; D002939:Ciprofloxacin / Q000008:administration & dosage*; D006801:Humans; D011513:Proteus mirabilis / Q000187:drug effects / Q000254:growth & development / Q000472:pathogenicity; D014552:Urinary Tract Infections / Q000382:microbiology
chemical_list: D002939:Ciprofloxacin; D002442:Ceftazidime