title: Specific recognition of a tetrahedral phosphonamidate transition state analogue group by a recombinant antibody Fab fragment.
abstract: In order to obtain antibodies able to catalyse a peptide synthesis, a naive combinatorial library of human Fab antibody fragments was screened with the phosphonamidate transition state analogue of the reaction. Several Fab fragments were able to bind the analogue. Competitive binding studies performed with molecules containing representative parts of the hapten showed that two Fabs were able to recognize specifically the tetrahedral phosphorus present in the hapten.
authors.affiliation: Laboratoire des Aminoacides et Peptides, CNRS-URA 468, Université Montpellier II, CNRS 1919 route de Mende, F-34033, Montpellier Cedex 1, France.
authors.forename: T D
authors.initials: TD
authors.lastname: Hua
authors.forename: F
authors.initials: F
authors.lastname: Lamaty
authors.forename: C
authors.initials: C
authors.lastname: Souriau
authors.forename: V
authors.initials: V
authors.lastname: Rolland-Fulcrand
authors.forename: R
authors.initials: R
authors.lastname: Lazaro
authors.forename: P
authors.initials: P
authors.lastname: Viallefont
authors.forename: M P
authors.initials: MP
authors.lastname: Lefranc
authors.forename: M
authors.initials: M
authors.lastname: Weill