title: A reversible gene trap collection empowers haploid genetics in human cells.
abstract: Knockout collections are invaluable tools for studying model organisms such as yeast. However, there are no large-scale knockout collections of human cells. Using gene-trap mutagenesis in near-haploid human cells, we established a platform to generate and isolate individual 'gene-trapped cells' and used it to prepare a collection of human cell lines carrying single gene-trap insertions. In most cases, the insertion can be reversed. This growing library covers 3,396 genes, one-third of the expressed genome, is DNA-barcoded and allows systematic screens for a wide variety of cellular phenotypes. We examined cellular responses to TNF-α, TGF-β, IFN-γ and TNF-related apoptosis-inducing ligand (TRAIL), to illustrate the value of this unique collection of isogenic human cell lines.
authors.affiliation: Haplogen GmbH, Vienna, Austria.
authors.forename: Tilmann
authors.initials: T
authors.lastname: Bürckstümmer
authors.forename: Carina
authors.initials: C
authors.lastname: Banning
authors.forename: Philipp
authors.initials: P
authors.lastname: Hainzl
authors.forename: Richard
authors.initials: R
authors.lastname: Schobesberger
authors.forename: Claudia
authors.initials: C
authors.lastname: Kerzendorfer
authors.forename: Florian M
authors.initials: FM
authors.lastname: Pauler
authors.forename: Doris
authors.initials: D
authors.lastname: Chen
authors.forename: Nicole
authors.initials: N
authors.lastname: Them
authors.forename: Fiorella
authors.initials: F
authors.lastname: Schischlik
authors.forename: Manuele
authors.initials: M
authors.lastname: Rebsamen
authors.forename: Michal
authors.initials: M
authors.lastname: Smida
authors.forename: Ferran
authors.initials: F
authors.lastname: Fece de la Cruz
authors.forename: Ana
authors.initials: A
authors.lastname: Lapao
authors.forename: Melissa
authors.initials: M
authors.lastname: Liszt
authors.forename: Benjamin
authors.initials: B
authors.lastname: Eizinger
authors.forename: Philipp M
authors.initials: PM
authors.lastname: Guenzl
authors.forename: Vincent A
authors.initials: VA
authors.lastname: Blomen
authors.forename: Tomasz
authors.initials: T
authors.lastname: Konopka
authors.forename: Bianca
authors.initials: B
authors.lastname: Gapp
authors.forename: Katja
authors.initials: K
authors.lastname: Parapatics
authors.forename: Barbara
authors.initials: B
authors.lastname: Maier
authors.forename: Johannes
authors.initials: J
authors.lastname: Stöckl
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Fischl
authors.forename: Sejla
authors.initials: S
authors.lastname: Salic
authors.forename: M Rita
authors.initials: MR
authors.lastname: Taba Casari
authors.forename: Sylvia
authors.initials: S
authors.lastname: Knapp
authors.forename: Keiryn L
authors.initials: KL
authors.lastname: Bennett
authors.forename: Christoph
authors.identifier: 0000000160913088
authors.initials: C
authors.lastname: Bock
authors.forename: Jacques
authors.initials: J
authors.lastname: Colinge
authors.forename: Robert
authors.initials: R
authors.lastname: Kralovics
authors.forename: Gustav
authors.initials: G
authors.lastname: Ammerer
authors.forename: Georg
authors.initials: G
authors.lastname: Casari
authors.forename: Thijn R
authors.initials: TR
authors.lastname: Brummelkamp
authors.forename: Giulio
authors.initials: G
authors.lastname: Superti-Furga
authors.forename: Sebastian M B
authors.initials: SM
authors.lastname: Nijman
mesh_terms: D045744:Cell Line, Tumor; D015723:Gene Library; D015894:Genome, Human; D006238:Haploidy; D006801:Humans; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods*; D059386:Reverse Genetics / Q000379:methods*