title: Corni Fructus Containing Formulation Attenuates Weight Gain in Mice with Diet-Induced Obesity and Regulates Adipogenesis through AMPK.
abstract: Obesity is a metabolic disorder characterized by chronic inflammation and dyslipidemia and is a strong predictor for the development of hypertension, diabetes mellitus, and cardiovascular disease. This study examined the antiobesity effect of an ethanol extract of Corni Fructus containing formulation (CDAP), which is a combination of four natural components: Corni Fructus, Dioscoreae Rhizoma, Aurantii Fructus Immaturus, and Platycodonis Radix. The cellular lipid content in 3T3-L1 adipocytes was assessed by Oil Red O staining. Expressions of peroxisome proliferator-activated receptor- γ (PPAR- γ ), CCAAT/enhancer-binding protein- α (C/EBP- α ), and lipin-1 were determined by real-time RT-PCR. Western blot was used to determine the protein levels of PPAR- γ , C/EBP- α , and AMP-activated protein kinase- α (AMPK- α ). The CDAP extract suppressed the differentiation of 3T3-L1 adipocytes by downregulating cellular induction of PPAR- γ , C/EBP- α , and lipin-1. The CDAP extract also significantly upregulated phosphorylation of AMPK- α . An in vivo study showed that CDAP induced weight loss in mice with high-fat-diet-induced obesity. These results indicate that CDAP has a potent anti-obesity effect due to the inhibition of adipocyte differentiation and adipogenesis.
authors.affiliation: College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, Hoegi-Dong, Dongdaemun-Gu, Seoul 130-701, Republic of Korea.
authors.forename: Hye-Lin
authors.initials: HL
authors.lastname: Kim
authors.forename: Yong-Deok
authors.initials: YD
authors.lastname: Jeon
authors.forename: Jinbong
authors.initials: J
authors.lastname: Park
authors.forename: Hong-Kun
authors.initials: HK
authors.lastname: Rim
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Jeong
authors.forename: Hara
authors.initials: H
authors.lastname: Lim
authors.forename: Seong-Gyu
authors.initials: SG
authors.lastname: Ko
authors.forename: Hyeung-Jin
authors.initials: HJ
authors.lastname: Jang
authors.forename: Byung-Cheol
authors.initials: BC
authors.lastname: Lee
authors.forename: Kyung-Tae
authors.initials: KT
authors.lastname: Lee
authors.forename: Kang-Min
authors.initials: KM
authors.lastname: Lee
authors.forename: Hyejung
authors.initials: H
authors.lastname: Lee
authors.forename: Sung-Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Hong
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Um