title: A fast and feasible microextraction by packed sorbent (MEPS) procedure for HPLC analysis of the atypical antipsychotic ziprasidone in human plasma.
abstract: An original high-performance liquid chromatographic method coupled to microextraction by packed sorbent (MEPS) was developed for the therapeutic drug monitoring (TDM) of psychiatric patients treated with the recent atypical antipsychotic ziprasidone. The chromatographic separation was achieved on a RP C18 column, using an isocratic mobile phase and setting the wavelength at 320nm. The analyte was extracted from human plasma by means of a fast and feasible innovative MEPS procedure, optimised on C2 sorbent and requiring only 100μL of biological sample. A second pre-treatment procedure, based on solid phase extraction (SPE), has been also developed for comparison. The availability of different pre-treatment procedures allows the choice of the one best suiting the specific clinical, economic and scientific needs. The extraction yield values were always higher than 90% and sensitivity was also good, with a limit of quantitation (LOQ) of 1ng/mL. The method was successfully applied to plasma samples from ten subjects undergoing therapy with ziprasidone, thus confirming its suitability for the TDM of psychiatric patients, in order to personalise their pharmacological treatments.
authors.affiliation: Laboratory of Pharmaco-Toxicological Analysis, Department of Pharmacy and Biotechnology (FaBiT), Alma Mater Studiorum - University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy. Electronic address: laura.mercolini@unibo.it.
authors.forename: Laura
authors.initials: L
authors.lastname: Mercolini
authors.forename: Michele
authors.initials: M
authors.lastname: Protti
authors.forename: Giulia
authors.initials: G
authors.lastname: Fulgenzi
authors.forename: Roberto
authors.initials: R
authors.lastname: Mandrioli
authors.forename: Nadia
authors.initials: N
authors.lastname: Ghedini
authors.forename: Andreas
authors.initials: A
authors.lastname: Conca
authors.forename: Maria Augusta
authors.initials: MA
authors.lastname: Raggi
mesh_terms: D014150:Antipsychotic Agents / Q000032:analysis*; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D016903:Drug Monitoring; D006801:Humans; D057230:Limit of Detection; D010879:Piperazines / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction / Q000379:methods*; D013844:Thiazoles / Q000737:chemistry*
chemical_list: D014150:Antipsychotic Agents; D010879:Piperazines; D013844:Thiazoles; C092292:ziprasidone
keywords: HPLC; Microextraction by packed sorbent (MEPS); Plasma; Therapeutic drug monitoring (TDM); Ziprasidone