title: The siRNA cocktail targeting VEGF and HER2 inhibition on the proliferation and induced apoptosis of gastric cancer cell.
abstract: The aim of this study was to investigate the inhibitory effect of a siRNA cocktail targeting Vascular endothelial growth factor (VEGF) and Human epidermal growth factor receptor 2 (HER2) on cell proliferation, induced apoptosis and the expression of VEGF and HER2 in human gastric carcinoma cell. The silencing rate of pre-designed siRNAs that targeted VEGF and HER2 was detected by Real-time Quantitative PCR (RT-QPCR) analysis. Furthermore, the best silencing siRNA that targeted VEGF and HER2 was prepared as a cocktail to co-knockdown VEGF and HER2 expression at both mRNA and protein levels which were detected by RT-QPCR and Western blot analysis. Cell proliferation inhibition rates were determined by CCK8 assay. The effect of siRNA cocktail on cell apoptosis was determined by flow cytometry. The migration inhibition of siRNA cocktail was analyzed by wound-healing assay. The ability of VEGF to induce endothelial cells to proliferate was examined in HUVECs by the method of tube formation assay. The pre-designed siRNAs could inhibit VEGF and HER2 mRNA level. siRNA cocktail, and co-downregulation of VEGF and HER2 result in significant inhibition of gastric cancer growth and migration in vitro. The inhibition of VEGF and HER2 expressions can induce apoptosis of SGC-7901 cells.
authors.affiliation: Department of Cardiothoracic Surgery, The Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.forename: Honglin
authors.initials: H
authors.lastname: Chen
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: You
authors.forename: Hai
authors.initials: H
authors.lastname: Shi
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018734:Genes, erbB-2; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics*; D013274:Stomach Neoplasms / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*; D014945:Wound Healing
chemical_list: D034741:RNA, Small Interfering; D042461:Vascular Endothelial Growth Factor A