title: Evaluation of environmental fecal culture for Mycobacterium avium subspecies paratuberculosis detection in dairy herds and association with apparent within-herd prevalence.
abstract: This study evaluated test characteristics of environmental culture (EC) for the detection of Mycobacterium avium subspecies paratuberculosis (MAP) in 32 herds over a 2-year period. Individual fecal samples were collected every 6 mo and environmental samples every 3 mo. Individual fecal culture was performed on samples from positive pools. Samples were cultured in broth, with confirmatory polymerase chain reaction performed on positive fecal samples. Repeated measures were accounted for using GEE logistic models. Relative to a MAP herd-status based on all pooled fecal culture results collected during the study, sensitivity of a set of 6 EC-samples collected from prescribed locations within the herd environment (EC-6) was 71% [95% confidence interval (CI): 49% to 86%] and specificity was 99% (95% CI: 95% to 100%). Sensitivity of EC increased as apparent within-herd fecal culture prevalence (aWHP) increased. The estimated aWHP increased as the proportion of positive EC-samples within an EC-6 set increased. Environmental culture is an acceptable tool for herd diagnosis of MAP in low-prevalence herds.
authors.affiliation: Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Prince Edward Island (Lavers, McKenna, Dohoo, Keefe); Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta (Barkema).
authors.forename: Carrie J
authors.initials: CJ
authors.lastname: Lavers
authors.forename: Shawn L B
authors.initials: SL
authors.lastname: McKenna
authors.forename: Ian R
authors.initials: IR
authors.lastname: Dohoo
authors.forename: Herman W
authors.initials: HW
authors.lastname: Barkema
authors.forename: Greg P
authors.initials: GP
authors.lastname: Keefe
mesh_terms: D000818:Animals; D002170:Canada / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000382:microbiology*; D003612:Dairying; D005243:Feces / Q000382:microbiology*; D005260:Female; D016927:Mycobacterium avium subsp. paratuberculosis / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000175:diagnosis / Q000382:microbiology*; D015995:Prevalence