title: The insect-phase gRNA transcriptome in Trypanosoma brucei.
abstract: One of the most striking examples of small RNA regulation of gene expression is the process of RNA editing in the mitochondria of trypanosomes. In these parasites, RNA editing involves extensive uridylate insertions and deletions within most of the mitochondrial messenger RNAs (mRNAs). Over 1200 small guide RNAs (gRNAs) are predicted to be responsible for directing the sequence changes that create start and stop codons, correct frameshifts and for many of the mRNAs generate most of the open reading frame. In addition, alternative editing creates the opportunity for unprecedented protein diversity. In Trypanosoma brucei, the vast majority of gRNAs are transcribed from minicircles, which are approximately one kilobase in size, and encode between three and four gRNAs. The large number (5000-10,000) and their concatenated structure make them difficult to sequence. To identify the complete set of gRNAs necessary for mRNA editing in T. brucei, we used Illumina deep sequencing of purified gRNAs from the procyclic stage. We report a near complete set of gRNAs needed to direct the editing of the mRNAs.
authors.affiliation: Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA, Department of Computer Science and Engineering, Michigan State University, East Lansing, MI 48824, USA and Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Donna
authors.initials: D
authors.lastname: Koslowsky
authors.forename: Yanni
authors.initials: Y
authors.lastname: Sun
authors.forename: Jordan
authors.initials: J
authors.lastname: Hindenach
authors.forename: Terence
authors.initials: T
authors.lastname: Theisen
authors.forename: Jasmin
authors.initials: J
authors.lastname: Lucas
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics; D001483:Base Sequence; D045303:Cytochromes b / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics; D017393:RNA Editing; D017394:RNA, Guide, Kinetoplastida / Q000737:chemistry* / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics; D024363:Transcription Initiation Site; D059467:Transcriptome; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D012269:Ribosomal Proteins; C034212:ribosomal protein S12; D045303:Cytochromes b; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV; D000251:Adenosine Triphosphatases; D017394:RNA, Guide, Kinetoplastida