title: Independent and simultaneous three-dimensional optical trapping and imaging.
abstract: Combining imaging and control of multiple micron-scaled objects in three dimensions opens up new experimental possibilities such as the fabrication of colloidal-based photonic devices, as well as high-throughput studies of single cell dynamics. Here we utilize the dual-objectives approach to combine 3D holographic optical tweezers with a spinning-disk confocal microscope. Our setup is capable of trapping multiple different objects in three dimensions with lateral and axial accuracy of 8 nm and 20 nm, and precision of 20 nm and 200 nm respectively, while imaging them in four different fluorescence channels. We demonstrate fabrication of ordered two-component and three dimensional colloidal arrays, as well as trapping of yeast cell arrays. We study the kinetics of the division of yeast cells within optical traps, and find that the timescale for division is not affected by trapping.
authors.affiliation: School of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Tel Aviv 69978, Israel ; Contributed equally to this manuscript and should be considered joint first authors.
authors.forename: Maya
authors.initials: M
authors.lastname: Yevnin
authors.forename: Dror
authors.initials: D
authors.lastname: Kasimov
authors.forename: Yael
authors.initials: Y
authors.lastname: Gluckman
authors.forename: Yuval
authors.initials: Y
authors.lastname: Ebenstein
authors.forename: Yael
authors.initials: Y
authors.lastname: Roichman
keywords: (090.1760) Computer holography; (120.4610) Optical fabrication; (140.7010) Laser trapping; (180.1790) Confocal microscopy