title: Effects of polymorphisms in pepsinogen (PEP), amylase (AMY) and trypsin (TRY) genes on food habit domestication traits in mandarin fish.
abstract: Mandarin fish (Siniperca chuatsi) have a peculiar feeding habit of only accepting live fish prey and refusing dead prey and artificial diets. However, previous research has shown that some individuals accept dead prey after gradual domestication. Digestive enzymes are correlated with feeding habits in fish. In the current study, SNPs in the mandarin fish genes for pepsinogen (PEP), amylase (AMY), and trypsin (TRY) were evaluated for associations with feeding habits in domesticated mandarin fish by scanning their complete genomic sequence. In total, two SNPs were found in PEP, one was found in TRY, and none were found in AMY. The D1(CTCC) and D5(TTTT) diplotypes in the PEP gene tended to show strong effects on the feeding habits of domesticated fish (p < 0.01). The results indicate that PEP may be associated with the genetic mechanism for feeding habits in mandarin fish, and the D1(CTCC) and D5(TTTT) diplotypes in the PEP gene may be useful markers for selecting mandarin fish with appropriate feeding habits for domestication.
authors.affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan 430070, China. xufang_liang@hotmail.com.
authors.forename: Tilin
authors.initials: T
authors.lastname: Yi
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.forename: Xufang
authors.initials: X
authors.lastname: Liang
authors.forename: Shan
authors.initials: S
authors.lastname: He
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Zhengyong
authors.initials: Z
authors.lastname: Wen
authors.forename: Dan
authors.initials: D
authors.lastname: Shen
mesh_terms: D000681:Amylases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D000829:Animals, Domestic; D005247:Feeding Behavior; D005399:Fishes; D006801:Humans; D020116:Pepsinogen A / Q000235:genetics* / Q000378:metabolism; D010473:Perciformes / Q000235:genetics; D011110:Polymorphism, Genetic; D014357:Trypsin / Q000235:genetics* / Q000378:metabolism
chemical_list: D020116:Pepsinogen A; D000681:Amylases; D014357:Trypsin