title: Cell-based bioluminescence screening assays.
abstract: Drug screening is an essential and widely used technique for drug discovery in various biomedical fields notably in oncology. Here we describe a functional screening assay based on the bioluminescence detection of a secreted luciferase for monitoring cell viability of cancer cells in a high-throughput format. This assay allows the screening of large libraries comprising thousands of compounds and the identification of potential anticancer molecules in a rapid, facile, and cost-effective manner.
authors.affiliation: Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Boston, MA, USA.
authors.forename: Romain J
authors.initials: RJ
authors.lastname: Amante
authors.forename: Christian E
authors.initials: CE
authors.lastname: Badr
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival; D003362:Cost-Benefit Analysis; D004354:Drug Screening Assays, Antitumor / Q000191:economics / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008163:Luminescent Measurements; D014162:Transfection
chemical_list: D000970:Antineoplastic Agents; D008156:Luciferases