title: Polycation-assisted DNA detection by reduction triggered fluorescence amplification probe.
abstract: We have developed a fluorescence detection system for DNA, assisted by a comb-type cationic polymer (PLL-g-DX), for accelerating the reaction turnover. The combination of fluorogenic DNA probes with a comb-type cationic polymer has been demonstrated to be an effective means of signal amplification during the detection process. The method described herein represents a simple and enzyme-free detection.
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako, Saitama 351-0198, Japan; Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198, Japan.
authors.forename: Hisao
authors.initials: H
authors.lastname: Saneyoshi
authors.forename: Naohiko
authors.initials: N
authors.lastname: Shimada
authors.forename: Atsushi
authors.initials: A
authors.lastname: Maruyama
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Abe
mesh_terms: D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry; D010084:Oxidation-Reduction; D011073:Polyamines / Q000737:chemistry*; D000071228:Polyelectrolytes; D011107:Polylysine / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D004247:DNA; D015342:DNA Probes; D005456:Fluorescent Dyes; D009943:Organophosphorus Compounds; D011073:Polyamines; D000071228:Polyelectrolytes; D011107:Polylysine; C061896:triphenylphosphine
keywords: Catalyst; DNA; Fluorescence detection; Polymer; Reduction