title: Synthesis of nitric oxide probes with fluorescence lifetime sensitivity.
abstract: We present the rationale, synthesis and evaluation of the first activatable fluorescent probe that utilizes fluorescence lifetime change for detection of nitric oxide. The new probe DAP-LT1 features a near-infrared polymethine skeleton with a diaminobenzene functionality incorporated into the meso-position. The probe is partially quenched, and upon reaction with nitric oxide shows an increase in the fluorescence lifetime from 1.08 ns to 1.24 ns.
authors.affiliation: Department of Radiology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110, USA. berezinm@mir.wustl.edu.
authors.forename: Natalia G
authors.initials: NG
authors.lastname: Zhegalova
authors.forename: Garrett
authors.initials: G
authors.lastname: Gonzales
authors.forename: Mikhail Y
authors.initials: MY
authors.lastname: Berezin
mesh_terms: D002232:Carbocyanines / Q000138:chemical synthesis* / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D007211:Indoles / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D009569:Nitric Oxide / Q000032:analysis*; D013997:Time Factors
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; D007211:Indoles; D009569:Nitric Oxide