title: Th17 response to Dermatophagoides pteronyssinus is related to late-phase airway and systemic inflammation in allergic asthma.
abstract: BACKGROUND
  Th17 cells may play a role in the development of late-phase allergen-induced airway and systemic inflammation in allergic asthma, although the mechanisms involved remain to be elucidated.
  
  
  METHODS
  A total of 36 subjects were enrolled into the study: 15 allergic asthma patients with early asthmatic reaction (n=7) or dual asthmatic reaction (n=8) developed to inhaled D. pteronyssinus, 13 patients with allergic rhinitis, and 8 healthy subjects. Peripheral blood and induced sputum were collected 24h before as well as 7h and 24h after a bronchial challenge with D. pteronyssinus. Th17 cells were analyzed by FACS; IL-17 levels were determined by ELISA.
  
  
  RESULTS
  At baseline, the percentage of peripheral blood Th17 cells and serum and sputum IL-17 levels were significantly higher in all groups of studied patients compared with those of healthy subjects. After the bronchial challenge, there was a significant increase in the percentage of peripheral blood Th17 cells and in serum and sputum IL-17 levels in rhinitis and asthma patients compared with their baseline values, particularly in allergic asthma patients with the dual asthmatic reaction. Positive correlations were found between the percentage of Th17 cells and IL-17 levels in serum (Rs=0.649; P=0.009) as well in sputum (Rs=0.583; P=0.022) in allergic asthma patients 24h after the bronchial challenge.
  
  
  CONCLUSIONS
  The Th17 response is associated with the development of late-phase airway and systemic inflammation after the inhalation of D. pteronyssinus in patients with allergic asthma.
authors.affiliation: Department of Pulmonology and Immunology, Lithuanian University of Health Sciences, Lithuania. Electronic address: bajoriuniene.ieva@gmail.com.
authors.forename: Ieva
authors.initials: I
authors.lastname: Bajoriuniene
authors.forename: Kestutis
authors.initials: K
authors.lastname: Malakauskas
authors.forename: Simona
authors.initials: S
authors.lastname: Lavinskiene
authors.forename: Jolanta
authors.initials: J
authors.lastname: Jeroch
authors.forename: Raimundas
authors.initials: R
authors.lastname: Sakalauskas
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology*; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D001249:Asthma / Q000276:immunology* / Q000503:physiopathology; D001985:Bronchial Provocation Tests; D040002:Dermatophagoides pteronyssinus / Q000276:immunology*; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007249:Inflammation / Q000276:immunology / Q000503:physiopathology; D020381:Interleukin-17 / Q000097:blood / Q000276:immunology; D015848:Interleukin-5 / Q000276:immunology; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D012882:Skin Tests; D013147:Spirometry; D013183:Sputum / Q000276:immunology; D058504:Th17 Cells / Q000276:immunology; D055815:Young Adult
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D020381:Interleukin-17; D015848:Interleukin-5; D007073:Immunoglobulin E
keywords: Airway inflammation; Allergic asthma; Bronchial allergen challenge; Late-phase inflammation; Th17 cells