title: Fructose 1, 6-diphosphate regulates desmosomal proteins and collagen fibres in human skin equivalents.
abstract: We previously reported that fructose 1,6-diphosphate (FDP), a glycolytic metabolite, alleviates ultraviolet B-induced oxidative skin damage. Here, we further examined the effects of FDP on skin. FDP decreased the number of desmosomes, whereas it increased collagen fibres in skin equivalents (SEs). FDP significantly decreased the expression of corneodesmosomal components such as desmoglein 1 (DSG1), desmocollin 1 (DSC1) and corneodesmosin (CDSN), and desquamation-related proteases, kallikrein 5 (KLK 5) and kallikrein 7 (KLK7) in normal human epidermal keratinocytes (NHEKs). In addition, FDP treatment increased the phosphorylation of p38 MAPK, but the decreased expression of corneodesmosomal components is not recovered by the treatment of p38 MAPK inhibitors. Interestingly, FDP diminished the amplitude of Ca(2+) fluxes through down-regulation of SERCA2. Taken together, these results suggested that FDP induced a decrease in desmosomes and an increase in collagen fibres similar to the process of chemical peeling, the most common treatments for ageing skin.
authors.affiliation: Bioscience Research Institute, Amorepacific Corporation R&D Center, Yongin, Gyeonggi-do, Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Choi
authors.forename: Seung Ha
authors.initials: SH
authors.lastname: Yang
authors.forename: Il-Hong
authors.initials: IH
authors.lastname: Bae
authors.forename: Ju-Yearl
authors.initials: JY
authors.lastname: Park
authors.forename: Hyoung-June
authors.initials: HJ
authors.lastname: Kim
authors.forename: Minsoo
authors.initials: M
authors.lastname: Noh
authors.forename: Tae Ryong
authors.initials: TR
authors.lastname: Lee
authors.forename: Dong Wook
authors.initials: DW
authors.lastname: Shin
mesh_terms: D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D051187:Desmocollins / Q000378:metabolism; D051183:Desmoglein 1 / Q000378:metabolism; D003896:Desmosomes / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism*; D005635:Fructosediphosphates / Q000494:pharmacology*; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D007610:Kallikreins / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology; D010766:Phosphorylation; D015595:Skin Aging / Q000187:drug effects; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: C109656:CDSN protein, human; C081560:DSC1 protein, human; D051187:Desmocollins; D051183:Desmoglein 1; D004791:Enzyme Inhibitors; D005635:Fructosediphosphates; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D003094:Collagen; D048051:p38 Mitogen-Activated Protein Kinases; C400147:KLK5 protein, human; C082472:KLK7 protein, human; D007610:Kallikreins; C029063:fructose-1,6-diphosphate
keywords: collagen; desmosome; fructose 1,6-diphosphate; normal human epidermal keratinocytes; skin equivalents