title: Apolipoprotein A5 T-1131C variant and risk for metabolic syndrome in obese adolescents.
abstract: BACKGROUND
  Apolipoprotein A5 (APOA5) gene variants are associated with increased plasma triglycerides, a risk factor for metabolic syndrome (MS). The goal of the current study was the investigation of the distribution of T-1131C variant among obese adolescents with MS compared with healthy controls.
  
  
  METHODS
  The study included 150 obese adolescents (75 males and 75 females) with MS and 204 age and sex matched normal healthy controls (100 males and 104 females). The mean age of the patients was 15.47 ± 2.54 years, ranged from 17 to 20 years. They were genotyped by polymerase chain reaction-restriction fragment length polymorphism for the mutation (T-1131C).
  
  
  RESULTS
  The blood pressure, triglyceride and HOMA-R levels were significantly higher and HDL-C levels were significantly lower in carrier (TC+CC) compared to non-carrier (TT) MS patients. There was accumulation of -1131C allele frequency in the MS group (31.33% vs. control group 11.76%), p<0.001. The genotypes were in Hardy-Weinberg equilibrium both in the patients with metabolic syndrome and in the control subjects. Results of analysis of multiple regression models showed that the ApoA5 -1131C carriers showed an increased incidence of MS (OR=1.73, 95% CI: 1.41-2.11).
  
  
  CONCLUSIONS
  The present study suggests that the 1131T>C polymorphism is a risk factor for the development of metabolic syndrome in obese adolescents.
authors.affiliation: Biological Anthropology, Medical Research Division, National Research Centre, Egypt. Electronic address: moushiraz@yahoo.com.
authors.forename: Moushira
authors.initials: M
authors.lastname: Zaki
authors.forename: Khalda
authors.initials: K
authors.lastname: Amr
mesh_terms: D000293:Adolescent; D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D024821:Metabolic Syndrome / Q000235:genetics* / Q000378:metabolism; D009765:Obesity / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D055815:Young Adult
chemical_list: C437704:APOA5 protein, human; D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A
keywords: ApoA5 T-1131C; ApoB; BMI; BP; CI; DNA; HDL-C; HOMA-R; IDF; International Diabetes Federation; LDL-C; MS; Metabolic syndrome; OR; Obesity; PCR; SNPs; SPSS; Statistical Package for the Social Sciences; TGs; VLDL; WC; apolipoprotein B; blood pressure; body mass index; confidence interval; dNTP; deoxyribonucleic acid; deoxyribonucleoside triphosphate; high-density lipoprotein cholesterol; homeostasis model assessment for insulin resistance; low-density lipoprotein cholesterol; metabolic syndrome; nucleotide polymorphisms; odds ratio; polymerase chain reaction; sdLDL; small dense low-density lipoprotein; triglycerides; very low density lipoprotein; waist circumference