title: High-throughput analysis of rice genes by means of the heterologous full-length cDNA overexpressor (FOX)-hunting system.
abstract: Mutant populations are indispensable tools for investigating plant gene functions. Gain-of-function technology is one of the approaches used for the systematic production of mutant resources and activation tagging is a well-established method to generate gain-of-function mutants in plants. As an alternative approach for the systematic generation of a gain-of-function mutant population, we developed the Full-length cDNA OvereXpressor (FOX)-hunting system in which full-length cDNAs (fl-cDNAs) are overexpressed in plants to quickly identify candidate genes. The FOX-hunting system was used for high-throughput analysis of rice (Oryza sativa) genes heterologously expressed in Arabidopsis thaliana (rice FOX Arabidopsis lines). A large screening to identify and characterize rice genes with rice FOX Arabidopsis lines revealed that one of the isolated genes, BROAD-SPECTRUM RESISTANCE 1 (BSR1) conferred multiple or broad-spectrum disease resistance in both a dicotyledonous and monocotyledonous plant. We found that expression of rice fl-cDNAs without a homolog in Arabidopsis affected morphological traits. In addition, overexpression of homologous genes of rice and Arabidopsis led to a similar phenotype. Thus, we conclude that the FOX-hunting system is an excellent heterologous system and offers a new tool with which to explore gene function in rice.
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan.
authors.forename: Mieko
authors.initials: M
authors.lastname: Higuchi-Takeuchi
authors.forename: Masaki
authors.initials: M
authors.lastname: Mori
authors.forename: Minami
authors.initials: M
authors.lastname: Matsui
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics; D005821:Genetic Techniques; D023281:Genomics / Q000379:methods; D057166:High-Throughput Screening Assays / Q000379:methods*; D009154:Mutation; D012275:Oryza / Q000235:genetics*; D010641:Phenotype; D018517:Plant Roots; D030821:Plants, Genetically Modified / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant