title: Inhibitory Effect of Chrysanthemum zawadskii Herbich var. latilobum Kitamura Extract on RANKL-Induced Osteoclast Differentiation.
abstract: Chrysanthemum zawadskii Herbich var. latilobum Kitamura, known as "Gujulcho" in Korea, has been used in traditional medicine to treat various inflammatory diseases, including rheumatoid arthritis. However, these effects have not been tested on osteoclasts, the bone resorbing cells that regulate bone metabolism. Here, we investigated the effects of C. zawadskii Herbich var. latilobum Kitamura ethanol extract (CZE) on osteoclast differentiation induced by treatment with the receptor activator of NF- κ B ligand (RANKL). CZE inhibited osteoclast differentiation and formation in a dose-dependent manner. The inhibitory effect of CZE on osteoclastogenesis was due to the suppression of ERK activation and the ablation of RANKL-stimulated Ca(2+)-oscillation via the inactivation of PLC γ 2, followed by the inhibition of CREB activation. These inhibitory effects of CZE resulted in a significant repression of c-Fos expression and a subsequent reduction of NFATc1, a key transcription factor for osteoclast differentiation, fusion, and activation in vitro and in vivo. These results indicate that CZE negatively regulates osteoclast differentiation and may be a therapeutic candidate for the treatment of various bone diseases, such as postmenopausal osteoporosis, rheumatoid arthritis, and periodontitis.
authors.affiliation: Center for Metabolic Function Regulation (CMFR), Wonkwang University School of Medicine, Iksan 570-749, Republic of Korea ; Department of Oral Microbiology and Immunology, College of Dentistry, Wonkwang University, Iksan 570-749, Republic of Korea.
authors.forename: Dong Ryun
authors.initials: DR
authors.lastname: Gu
authors.forename: Jin-Ki
authors.initials: JK
authors.lastname: Hwang
authors.forename: Munkhsoyol
authors.initials: M
authors.lastname: Erkhembaatar
authors.forename: Kang-Beom
authors.initials: KB
authors.lastname: Kwon
authors.forename: Min Seuk
authors.initials: MS
authors.lastname: Kim
authors.forename: Young-Rae
authors.initials: YR
authors.lastname: Lee
authors.forename: Seoung Hoon
authors.initials: SH
authors.lastname: Lee