title: Association of RFLP markers with loci conferring broad-based resistance to the soybean cyst nematode (Heterodera glycines).
abstract: Soybean cyst nematode (SCN) is a major soybean yield-limiting pest. The present study was conducted to map broad-based SCN resistance loci from the cultivar 'Hartwig'. Two-hundred F2∶3 lines derived from the cross 'Williams 82' x 'Hartwig' were screened with a fourth-generation SCN inbred and 56 polymorphic molecular markers. Allele states and phenotypes were analyzed using stepwise regression and the model selection was made at P [Symbol: see text] 0.01. Four unlinked RFLP markers (A006, A567, A487, A112) were associated with SCN resistance and the partial coefficient of determinations (R(2)) were 91%, 1%, 1%, and 1%. We have mapped a new, major SCN resistance locus (A006) and three minor loci (A567, A487, A112). This complete mapping will accelerate the transfer of broad-based resistance without linkage drag and aid in the determination of relationships among various SCN-resistant germplasm sources.
authors.affiliation: Indiana Crop Improvement Association and Department of Agronomy, Purdue University, 47907-1150, West Lafayette, IN, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Vierling
authors.forename: J
authors.initials: J
authors.lastname: Faghihi
authors.forename: V R
authors.initials: VR
authors.lastname: Ferris
authors.forename: J M
authors.initials: JM
authors.lastname: Ferris