title: Human augmenter of liver regeneration: probing the catalytic mechanism of a flavin-dependent sulfhydryl oxidase.
abstract: Augmenter of liver regeneration is a member of the ERV family of small flavin-dependent sulfhydryl oxidases that contain a redox-active CxxC disulfide bond in redox communication with the isoalloxazine ring of bound FAD. These enzymes catalyze the oxidation of thiol substrates with the reduction of molecular oxygen to hydrogen peroxide. This work studies the catalytic mechanism of the short, cytokine form of augmenter of liver regeneration (sfALR) using model thiol substrates of the enzyme. The redox potential of the proximal disulfide in sfALR was found to be approximately 57 mV more reducing than the flavin chromophore, in agreement with titration experiments. Rapid reaction studies show that dithiothreitol (DTT) generates a transient mixed disulfide intermediate with sfALR signaled by a weak charge-transfer interaction between the thiolate of C145 and the oxidized flavin. The subsequent transfer of reducing equivalents to the flavin ring is relatively slow, with a limiting apparent rate constant of 12.4 s(-1). However, reoxidation of the reduced flavin by molecular oxygen is even slower (2.3 s(-1) at air saturation) and thus largely limits turnover at 5 mM DTT. The nature of the charge-transfer complexes observed with DTT was explored using a range of simple monothiols to mimic the initial nucleophilic attack on the proximal disulfide. While β-mercaptoethanol is a very poor substrate of sfALR (∼0.3 min(-1) at 100 mM thiol), it rapidly generates a mixed disulfide intermediate allowing the thiolate of C145 to form a strong charge-transfer complex with the flavin. Unlike the other monothiols tested, glutathione is unable to form charge-transfer complexes and is an undetectable substrate of the oxidase. These data are rationalized on the basis of the stringent steric requirements for thiol-disulfide exchange reactions. The inability of the relatively bulky glutathione to attain the in-line geometry required for efficient disulfide exchange in sfALR may be physiologically important in preventing the oxidase from catalyzing the potentially harmful oxidation of intracellular glutathione.
authors.affiliation: Department of Chemistry and Biochemistry, University of Delaware , Newark, Delaware 19716-2522, United States.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Schaefer-Ramadan
authors.forename: Shawn A
authors.initials: SA
authors.lastname: Gannon
authors.forename: Colin
authors.initials: C
authors.lastname: Thorpe
mesh_terms: D002384:Catalysis; D003579:Cytochrome Reductases / Q000235:genetics / Q000378:metabolism*; D004220:Disulfides / Q000737:chemistry; D005415:Flavins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008623:Mercaptoethanol / Q000378:metabolism; D008958:Models, Molecular; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D050862:Oxidoreductases Acting on Sulfur Group Donors; D010100:Oxygen / Q000737:chemistry
chemical_list: D003579:Cytochrome Reductases; D004220:Disulfides; D005415:Flavins; D008623:Mercaptoethanol; D010088:Oxidoreductases; D050862:Oxidoreductases Acting on Sulfur Group Donors; D010100:Oxygen; C008173:isoalloxazine; C500897:GFER protein, human