title: Hemagglutinin receptor specificity and structural analyses of respiratory droplet-transmissible H5N1 viruses.
abstract: Two ferret-adapted H5N1 viruses capable of respiratory droplet transmission have been reported with mutations in the hemagglutinin receptor-binding site and stalk domains. Glycan microarray analysis reveals that both viruses exhibit a strong shift toward binding to "human-type" α2-6 sialosides but with notable differences in fine specificity. Crystal structure analysis further shows that the stalk mutation causes no obvious perturbation of the receptor-binding pocket, consistent with its impact on hemagglutinin stability without affecting receptor specificity.
authors.affiliation: Departments of Cell and Molecular Biology and Chemical Physiology, The Scripps Research Institute, La Jolla, California, USA.
authors.forename: Robert P
authors.initials: RP
authors.lastname: de Vries
authors.forename: Xueyong
authors.initials: X
authors.lastname: Zhu
authors.forename: Ryan
authors.initials: R
authors.lastname: McBride
authors.forename: Alan
authors.initials: A
authors.lastname: Rigter
authors.forename: Anthony
authors.initials: A
authors.lastname: Hanson
authors.forename: Gongxun
authors.initials: G
authors.lastname: Zhong
authors.forename: Masato
authors.initials: M
authors.lastname: Hatta
authors.forename: Rui
authors.initials: R
authors.lastname: Xu
authors.forename: Wenli
authors.initials: W
authors.lastname: Yu
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kawaoka
authors.forename: Cornelis A M
authors.initials: CA
authors.lastname: de Haan
authors.forename: Ian A
authors.initials: IA
authors.lastname: Wilson
authors.forename: James C
authors.initials: JC
authors.lastname: Paulson
mesh_terms: D006388:Hemagglutinins / Q000378:metabolism*; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000502:physiology*
chemical_list: D006388:Hemagglutinins