title: Regulation of onco and tumor suppressor MiRNAs by mTORC1 inhibitor PRP-1 in human chondrosarcoma.
abstract: Metastatic chondrosarcoma of mesenchymal origin is the second most common bone malignancy and does not respond either to chemotherapy or radiation; therefore, the search for new therapies is relevant and urgent. This study aimed to reveal the comparative analysis of miRNAs and their targets in human JJ012 chondrosarcoma cell line between control and experimental samples, treated with mTORC1 inhibitor, cytostatic antiproliferative proline-rich polypeptide (PRP-1). Examination of tumor-specific microRNA expression profiles has revealed widespread deregulation of these molecules in diverse cancers. It was reported that microRNAs can function as novel biomarkers for disease diagnostics and therapy, as well as a novel class of oncogenes and tumor suppressor genes. mTORC 1 inhibitor PRP-1 caused significant upregulation of tumor suppressors, such as miR20a, miR125b, and miR192; and downregulation of onco miRNAs, miR509-3p, miR589, miR490-3p, miR 550 in human chondrosarcoma JJ012 cell line.
authors.affiliation: Department of Orthopaedics, Miller School of Medicine, University of Miami, 1600 NW 10th Avenue, Suite 8012 (D27), Miami, FL, 33136, USA, kgaloian@med.miami.edu.
authors.forename: Karina A
authors.initials: KA
authors.lastname: Galoian
authors.forename: Toumy
authors.initials: T
authors.lastname: Guettouche
authors.forename: Biju
authors.initials: B
authors.lastname: Issac
authors.forename: Amir
authors.initials: A
authors.lastname: Qureshi
authors.forename: H T
authors.initials: HT
authors.lastname: Temple
mesh_terms: D023181:Antimicrobial Cationic Peptides; D001859:Bone Neoplasms / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002813:Chondrosarcoma / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D016147:Genes, Tumor Suppressor / Q000187:drug effects; D006801:Humans; D000076222:Mechanistic Target of Rapamycin Complex 1; D035683:MicroRNAs / Q000096:biosynthesis / Q000187:drug effects*; D046912:Multiprotein Complexes / Q000037:antagonists & inhibitors*; D009857:Oncogenes / Q000187:drug effects; D010455:Peptides / Q000494:pharmacology*; D058570:TOR Serine-Threonine Kinases / Q000037:antagonists & inhibitors*
chemical_list: D023181:Antimicrobial Cationic Peptides; D035683:MicroRNAs; D046912:Multiprotein Complexes; C540268:PRP-1 peptide; D010455:Peptides; D000076222:Mechanistic Target of Rapamycin Complex 1; D058570:TOR Serine-Threonine Kinases