title: Characterization of novel single-variety oxygenated sesquiterpenoid hop oil fractions via headspace solid-phase microextraction and gas chromatography-mass spectrometry/olfactometry.
abstract: The volatile composition of novel varietal oxygenated sesquiterpenoid hop oil fractions ("spicy essences") was characterized by headspace solid-phase microextraction in combination with gas chromatography-mass spectrometry. Oxygenated sesquiterpenes represent the major chemical compound class, accounting for at least 65% of the total volatile fraction. In addition to oxygenated sesquiterpenes, spicy hop essences consist of several ketones, sesquiterpene and monoterpene hydrocarbons, and a relatively high number of unidentified compounds. On the basis of their relative composition, spicy hop essences can be fully differentiated according to their varietal origin. Multidimensional gas chromatography in combination with time-of-flight mass spectrometry on spicy hop essence cv. Spalter Select further demonstrated the enormous complexity of this particular hop oil fraction. The aromagram obtained via gas chromatography-olfactometry comprised nine odor-active regions described in terms of "citrus", "green", "haylike", "earthy", "woody", and "spicy". 2-Undecanone, 2-tridecanone, γ-cadinene, α-calacorene, calarene, humuladienone, caryolan-1-ol, caryophyllene oxide enantiomers, and humulene epoxide II are tentatively identified in the odor-active zones.
authors.affiliation: KAHO Sint-Lieven (KU Leuven) , Faculty of Engineering Technology, Department of Microbial and Molecular Systems (M2S), Cluster Bio-engineering Technology (CBeT), Laboratory of Enzyme, Fermentation and Brewing Technology (EFBT), Gebroeders De Smetstraat 1, 9000 Gent, Belgium.
authors.forename: Filip
authors.initials: F
authors.lastname: Van Opstaele
authors.forename: Tatiana
authors.initials: T
authors.lastname: Praet
authors.forename: Guido
authors.initials: G
authors.lastname: Aerts
authors.forename: Luc
authors.initials: L
authors.lastname: De Cooman
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D027582:Humulus / Q000737:chemistry*; D015394:Molecular Structure; D064367:Olfactometry; D010938:Plant Oils / Q000737:chemistry*; D052617:Solid Phase Microextraction / Q000379:methods; D013729:Terpenes / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D010938:Plant Oils; D013729:Terpenes