title: A sensitive chemotaxis assay using a novel microfluidic device.
abstract: Existing chemotaxis assays do not generate stable chemotactic gradients and thus--over time--functionally measure only nonspecific random motion (chemokinesis). In comparison, microfluidic technology has the capacity to generate a tightly controlled microenvironment that can be stably maintained for extended periods of time and is, therefore, amenable to adaptation for assaying chemotaxis. We describe here a novel microfluidic device for sensitive assay of cellular migration and show its application for evaluating the chemotaxis of smooth muscle cells in a chemokine gradient.
authors.affiliation: Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA ; Department of Hepatobiliary Surgery, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.forename: Sunyoung
authors.initials: S
authors.lastname: Jang
authors.forename: Ovid C
authors.initials: OC
authors.lastname: Amadi
authors.forename: Koichi
authors.initials: K
authors.lastname: Shimizu
authors.forename: Richard T
authors.initials: RT
authors.lastname: Lee
authors.forename: Richard N
authors.initials: RN
authors.lastname: Mitchell
mesh_terms: D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D002633:Chemotaxis; D006801:Humans; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D012680:Sensitivity and Specificity