title: Reduction of fibrosis in dibutyltin dichloride-induced chronic pancreatitis using rat umbilical mesenchymal stem cells from Wharton's jelly.
abstract: OBJECTIVE
  The objective of this study was to investigate the effects of rat umbilical cord mesenchymal stem cells (UCMSCs) from Wharton's jelly on dibutyltin dichloride (DBTC)-induced chronic pancreatitis (CP) and subsequent pancreatic fibrosis in rats.
  
  
  METHODS
  A rat model of CP induced by DBTC was used. Male Sprague-Dawley rats were randomly divided into 4 groups: the control, DBTC, DBTC + UCMSCs, and control + UCMSC groups. Umbilical cord mesenchymal stem cells were administered intravenously on day 5 after the administration of DBTC. On days 14 and 28, the rats were evaluated morphologically and biochemically. The expression levels of inflammatory cytokines and chemokines in the pancreatic tissues of different groups were evaluated using quantitative real-time polymerase chain reaction. The activation of pancreatic stellate cells was estimated by immunochemistry and Western blot analysis of α-smooth muscle actin.
  
  
  RESULTS
  Umbilical cord mesenchymal stem cells were detected in inflamed pancreatic tissues. Umbilical cord mesenchymal stem cell treatment improved the histological scores and alleviated the fibrosis of pancreas samples, The expression of cytokines in the DBTC + UCMSC group was significantly lower than that in the DBTC group. Also, pancreatic stellate cell activation was inhibited by UCMSC treatment.
  
  
  CONCLUSIONS
  Xenogeneic transplantation of UCMSCs is a novel approach for the treatment of CP and subsequent fibrosis. Umbilical cord mesenchymal stem cells may be a promising therapeutic intervention for human CP in the future.
authors.affiliation: From the *Department of Gastroenterology, The Second Affiliated Hospital of Soochow University; †Department of Hepatology, The First Affiliated Hospital of Soochow University, Soochow; ‡Department of Gastroenterology, The Second People's Hospital of Huai'an, Huai'an; §Experimental Center, The Fifth People's Hospital of Soochow; ∥Experimental Center and ¶Department of Pathology, The Second Affiliated Hospital of Soochow University, Soochow, People's Republic of China.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Zhou
authors.forename: Mei-Lin
authors.initials: ML
authors.lastname: Li
authors.forename: Ai-Lan
authors.initials: AL
authors.lastname: Qin
authors.forename: Sheng-Xiang
authors.initials: SX
authors.lastname: Lv
authors.lastname: Wen-Tang
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Zhu
authors.forename: Lin-Yun
authors.initials: LY
authors.lastname: Li
authors.forename: Yun
authors.initials: Y
authors.lastname: Dong
authors.forename: Chuang-Ying
authors.initials: CY
authors.lastname: Hu
authors.forename: Duan-Min
authors.initials: DM
authors.lastname: Hu
authors.forename: Shao-Feng
authors.initials: SF
authors.lastname: Wang
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics; D005260:Female; D005355:Fibrosis / Q000235:genetics / Q000378:metabolism / Q000601:surgery; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D009130:Muscle, Smooth / Q000737:chemistry; D009947:Organotin Compounds; D010179:Pancreas / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D058954:Pancreatic Stellate Cells / Q000378:metabolism; D050500:Pancreatitis, Chronic / Q000139:chemically induced / Q000601:surgery*; D011247:Pregnancy; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014470:Umbilical Cord / Q000166:cytology* / Q000378:metabolism; D059631:Wharton Jelly / Q000166:cytology* / Q000378:metabolism
chemical_list: D000199:Actins; D016207:Cytokines; D009947:Organotin Compounds; C020735:dibutyldichlorotin