title: Evaluation of colorimetric detection methods for Shigella, Salmonella, and Vibrio cholerae by loop-mediated isothermal amplification.
abstract: We evaluated loop-mediated isothermal amplification end-point detection methods for Salmonella, Shigella, and Vibrio cholerae. Detection sensitivities were comparable to real-time PCR methods. The colorimetric dyes hydroxynaphthol blue and SYBR Green I showed increased sensitivity when compared to visual and automated turbidity readings. End-point colorimetric dyes promise great utility in developing settings.
authors.affiliation: Papua New Guinea Institute of Medical Research, Goroka, Papua New Guinea.
authors.forename: Kevin W
authors.initials: KW
authors.lastname: Soli
authors.forename: Monalisa
authors.initials: M
authors.lastname: Kas
authors.forename: Tobias
authors.initials: T
authors.lastname: Maure
authors.forename: Masahiro
authors.initials: M
authors.lastname: Umezaki
authors.forename: Ayako
authors.initials: A
authors.lastname: Morita
authors.forename: Peter M
authors.initials: PM
authors.lastname: Siba
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Greenhill
authors.forename: Paul F
authors.initials: PF
authors.lastname: Horwood
mesh_terms: D003124:Colorimetry / Q000379:methods*; D006801:Humans; D058889:Molecular Typing; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D012475:Salmonella / Q000145:classification / Q000235:genetics*; D012680:Sensitivity and Specificity; D012760:Shigella / Q000145:classification / Q000235:genetics*; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics*
keywords: Colorimetric detection; Hydroxynaphthol blue; LAMP; Loop-mediated isothermal amplification; SYBR Green; Salmonella; Shigella; Vibrio cholerae