title: [Relationship between polymorphisms of tumor necrosis factor alpha gene and primary myelodysplastic syndromes].
abstract: OBJECTIVE
  To investigate the association of single nucleus polymorphisms(SNP)of tumor necrosis factor alpha (TNF-α) gene (-308 G>A and -238 G>A genotypes) with susceptibility to primary myelodysplastic syndromes (MDS).
  
  
  METHODS
  Two SNPs (TNF-α-308 G>A,TNF-α-238 G>A) of TNF-α gene were detected by Taqman probes in 341 MDS patients and 365 unrelated-healthy controls.
  
  
  RESULTS
  Compared to healthy controls, the frequency of TNF-α-308 AA+AG genotype and A allele increased (18% vs 10%, P=0.015, 9% vs 5%, P=0.021, respectively) in refractory cytopenia with multilineage dysplasia (RCMD) patients. There was no correlation of TNF-α-308 G>A genotype and allele frequency between MDS and controls. No difference in the genotype and allele frequency of TNF-α-238 G>A were found between controls and MDS or the subtypes of MDS (P>0.05). We did not find any linkage between plasma level of TNF-α and TNF-α-308 G>A or TNF-α-238 G>A genotype. Statistic differences were observed between platelet count[58(1-611)×10⁹/L vs 90(7-352)×10⁹/L]and bone marrow blasts in MDS patients carrying TNF-α-308 G>A GG and AA+AG genotype (P=0.024, 0.019, respectively).
  
  
  CONCLUSIONS
  TNF-α-308 G>A polymorphism was correlated with susceptibility to MDS-RCMD.
authors.affiliation: Institute of Hematology and Blood Diseases Hospital, CAMS & PUMC, State Key Laboratory of Experimental Hematology, Tianjin 300020, China.
authors.forename: Jun-qing
authors.initials: JQ
authors.lastname: Xu
authors.forename: Jie-yu
authors.initials: JY
authors.lastname: Wang
authors.forename: Tie-jun
authors.initials: TJ
authors.lastname: Qin
authors.forename: Ze-feng
authors.initials: ZF
authors.lastname: Xu
authors.forename: Li-wei
authors.initials: LW
authors.lastname: Fang
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Pan
authors.forename: Nai-bo
authors.initials: NB
authors.lastname: Hu
authors.forename: Hong-li
authors.initials: HL
authors.lastname: Zhang
authors.forename: Shi-qiang
authors.initials: SQ
authors.lastname: Qu
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhi-jian
authors.initials: ZJ
authors.lastname: Xiao
mesh_terms: D016022:Case-Control Studies; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000235:genetics*
chemical_list: D014409:Tumor Necrosis Factor-alpha