title: Immunohistochemical evaluation of neuroreceptors in healthy and pathological temporo-mandibular joint.
abstract: OBJECTIVE
  A study was performed on the articular disk and periarticular tissues of the temporo-mandibular joint (TMJ) with immunohistochemical techniques to give evidence to the presence of neuroreceptors (NRec) in these sites.
  
  
  METHODS
  The study was carried out on tissue samples obtained from 10 subjects without TMJ disease and from 7 patients with severe TMJ arthritis and arthrosis. We use antibodies directed against following antigens: Gliofibrillary Acidic Protein (GFAP), Leu-7, Myelin Basic Protein (MBP), Neurofilaments 68 kD (NF), Neuron Specific Enolase (NSE), S-100 protein (S-100) and Synaptophysin (SYN).
  
  
  RESULTS
  This study revealed that Ruffini's-like, Pacini's-like and Golgi's-like receptors can be demonstrated in TMJ periarticular tissues and that free nervous endings are present in the subsynovial tissues but not within the articular disk. We observed elongated cytoplamic processes of chondrocytes that demonstrated strong S-100 immunoreactivity but they were unreactive with all other antibodies. These cytoplamic processes were more abundant and thicker in the samples obtained from patients with disease TMJ.
  
  
  CONCLUSIONS
  The results of this study confirm that different Nrec are detectable in TMJ periarticular tissues but they are absent within the articular disk. In the latter site, only condrocytic processes are evident, especially in diseased TMJ, and they might have been confused with nervous endings in previous morphological studies. Nevertheless the absence of immunoreactivity for NF, NSE and SYN proves that they are not of neural origin.
authors.affiliation: 1. Full Professor, Dental School, University of Bari, Bari, Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Favia
authors.forename: Massimo
authors.initials: M
authors.lastname: Corsalini
authors.forename: Daniela
authors.initials: D
authors.lastname: Di Venere
authors.forename: Francesco
authors.initials: F
authors.lastname: Pettini
authors.forename: Giorgio
authors.initials: G
authors.lastname: Favia
authors.forename: Saverio
authors.initials: S
authors.lastname: Capodiferro
authors.forename: Eugenio
authors.initials: E
authors.lastname: Maiorano
mesh_terms: D000328:Adult; D000906:Antibodies / Q000276:immunology; D019902:Chondrocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D004676:Myelin Basic Protein / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D016900:Neurofilament Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D009418:S100 Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000276:immunology / Q000378:metabolism*; D016708:Synaptophysin / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D013704:Temporomandibular Joint / Q000276:immunology / Q000378:metabolism* / Q000473:pathology*; D013705:Temporomandibular Joint Disorders / Q000276:immunology / Q000378:metabolism* / Q000473:pathology*
chemical_list: D000906:Antibodies; D004676:Myelin Basic Protein; D016900:Neurofilament Proteins; D009418:S100 Proteins; D016708:Synaptophysin; C044882:neurofilament protein L; D010751:Phosphopyruvate Hydratase
keywords: neuroreceptors; temporo-mandibular joint