title: Taurine chloramine modulates the expression of adipokines through inhibition of the STAT-3 signaling pathway in differentiated human adipocytes.
abstract: To examine the possible role of taurine chloramine (TauCl) in modulating the expression of adipokines in adipose tissue associated with obesity, we evaluated the effect of TauCl in human differentiated adipocytes in response to IL-1β. To study the physiological effects of TauCl on adipokine expression, differentiated adipocytes were treated with IL-1β in the presence or absence of TauCl at concentrations ranging from 200 to 600 μM for 7 days. Cell culture supernatants and total RNA were analyzed by ELISA and real-time PCR, respectively, to determine protein and mRNA levels of adipokines, including adiponectin, leptin, IL-6, and IL-8. Levels of proteins involved in relevant signaling pathways were investigated by western blotting. Stimulation with IL-1β significantly decreased levels of adiponectin and leptin in adipocytes, but increased levels of IL-6 and IL-8 in a dose-dependent manner. Treatment with TauCl significantly reversed the modulation of adipokine expression by inhibiting STAT-3 signaling in IL-1β-stimulated adipocytes, independent of MAPK signaling. TauCl treatment more significantly modulated the expression of adipokines in adipocytes stimulated with IL-1β than that of non-stimulated adipocytes, suggesting that TauCl plays a significant role in modulating the expression of adipokines under inflammatory conditions. In conclusion, TauCl and other taurine derivatives that inhibit the STAT-3 signaling pathway can modulate expression of adipokines and thus may be useful as therapeutic agents for obesity-related diseases.
authors.affiliation: East-West Bone and Joint Research Institute, Kyung Hee University Hospital at Gangdong, Kyung Hee University, Seoul, 134-727, Republic of Korea, labrea46@yahoo.co.kr.
authors.forename: Kyoung Soo
authors.initials: KS
authors.lastname: Kim
authors.forename: Hye-In
authors.initials: HI
authors.lastname: Ji
authors.forename: Hyunju
authors.initials: H
authors.lastname: Chung
authors.forename: Chakyeun
authors.initials: C
authors.lastname: Kim
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Lee
authors.forename: Yeon-Ah
authors.initials: YA
authors.lastname: Lee
authors.forename: Hyung-In
authors.initials: HI
authors.lastname: Yang
authors.forename: Myung Chul
authors.initials: MC
authors.lastname: Yoo
authors.forename: Seung Jae
authors.initials: SJ
authors.lastname: Hong
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D054392:Adipokines / Q000096:biosynthesis* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013329:Structure-Activity Relationship; D013654:Taurine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D054392:Adipokines; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D013654:Taurine; C043410:N-chlorotaurine