title: Resolution and contrast enhancement in coherent anti-Stokes Raman-scattering microscopy.
abstract: We implement switching laser mode coherent anti-Stokes Raman-scattering (SLAM-CARS) microscopy to enhance the spatial resolution and contrast in label-free vibrational microscopy. The method, based on the intensity difference between two images obtained with Gaussian and doughnut-shaped modes, does not depend on the specimen and relies on minimal modifications of the typical CARS setup. We demonstrate subdiffraction resolution imaging of myelin sheaths in a mouse brainstem. A lateral resolution of 0.36λ(p) is achieved.
authors.forename: Alicja
authors.initials: A
authors.lastname: Gasecka
authors.forename: Amy
authors.initials: A
authors.lastname: Daradich
authors.forename: Harold
authors.initials: H
authors.lastname: Dehez
authors.forename: Michel
authors.initials: M
authors.lastname: Piché
authors.forename: Daniel
authors.initials: D
authors.lastname: Côté
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000166:cytology; D007834:Lasers; D051379:Mice; D008853:Microscopy / Q000379:methods*; D009186:Myelin Sheath / Q000378:metabolism; D055620:Optical Phenomena; D013059:Spectrum Analysis, Raman