title: Increasing the scale of peroxidase production by Streptomyces sp. strain BSII#1.
abstract: OBJECTIVE
  To optimize peroxidase production by Streptomyces sp. strain BSII#1, up to 3 l culture volumes.
  
  
  RESULTS
  Peroxidase production by Streptomyces sp. strain BSII#1 was optimized in terms of production temperature and pH and the use of lignin-based model chemical inducers. The highest peroxidase activity (1·30 ± 0·04 U ml(-1) ) in 10 ml culture volume was achieved in a complex production medium (pH 8·0) at 37°C in the presence of 0·1 mmol l(-1) veratryl alcohol, which was greater than those reported previously. Scale-up to 100 and 400 ml culture volumes resulted in decreased peroxidase production (0·53 ± 0·10 and 0·26 ± 0·08 U ml(-1) , respectively). However, increased aeration improved peroxidase production with the highest production achieved using an airlift bioreactor (4·76 ± 0·46 U ml(-1) in 3 l culture volume).
  
  
  CONCLUSIONS
  Veratryl alcohol (0·1 mmol l(-1) ) is an effective inducer of peroxidase production by Streptomyces sp. strain BSII#1. However, improved aeration increased peroxidase production in larger volumes without the use of an inducer, surpassing induced yields in an optimized small-scale process.
  
  
  CONCLUSIONS
  Only a limited number of reports in literature have focused on the up-scaling of bacterial peroxidase production. There remains opportunity for feasible large-scale production of bacterial peroxidases with potentially novel biocatalytic properties.
authors.affiliation: Biocatalysis and Technical Biology Research Group, Cape Peninsula University of Technology, Bellville, South Africa.
authors.forename: A
authors.initials: A
authors.lastname: Musengi
authors.forename: N
authors.initials: N
authors.lastname: Khan
authors.forename: M
authors.initials: M
authors.lastname: Le Roes-Hill
authors.forename: B I
authors.initials: BI
authors.lastname: Pletschke
authors.forename: S G
authors.initials: SG
authors.lastname: Burton
mesh_terms: D001592:Benzyl Alcohols / Q000378:metabolism; D019149:Bioreactors; D008031:Lignin / Q000378:metabolism; D009195:Peroxidase / Q000096:biosynthesis*; D013302:Streptomyces / Q000201:enzymology*; D013696:Temperature
chemical_list: D001592:Benzyl Alcohols; D008031:Lignin; D009195:Peroxidase; C042197:veratryl alcohol
keywords: actinomycete; biocatalysis; peroxidase