title: Segregation of heterogeneous rDNA segments during demagnification of a Neurospora crassa strain possessing a double nucleolar organizer.
abstract: We have produced a duplication strain of Neurospora crassa, DpAR33-PR-6, which contains two cytologically visible nucleoli (a DNO or double nucleolar organizer strain). When freshly generated, this strain has approximately twice the number of rRNA cistrons found in the parental (single nucleolar organizer) strains. After several serial propagations, there is a marked reduction in rRNA cistron number, approximating that of the SNO parental strains. This reduction in rRNA cistrons ("demagnification") was not achieved by breakdown of the VL→IVL translocation used to generate the duplication, as rDNA from the two parents can be distinguished by the size of the non-transcribed spacer region in the rDNA repeat unit of each strain. rDNA characteristic of both parents is present even after demagnification, in approximately equal amounts, suggesting the rRNA cistrons are lost randomly and non-preferentially from each homologous chromatid. In addition, the steady-state growth rate appears to be affected by rRNA cistron number, decreasing in freshly generated DNO strains relative to the parental strains, and returning to parental levels after demagnification.
authors.affiliation: Biology Department, Reed College, 97202, Portland, OR, USA.
authors.forename: K D
authors.initials: KD
authors.lastname: Rodland
authors.forename: P J
authors.initials: PJ
authors.lastname: Russell