title: Cilostazol-induced relaxation of calf cardiac vein and coronary artery during cooling.
abstract: OBJECTIVE
  At present very little is known about the role of endothelial nitric oxide (NO) in the effects of temperature on vascular reactivity. The aim of the present study is to evaluate the influence of cooling (to 28 °C) on the vasodilatation induced by cilostazol(10-9-3x10-4M) on carbachol (10-6)-precontracted calf cardiac vein and coronary artery and the role of NO in these effects.
  
  
  METHODS
  Ring preparations of great cardiac vein and the anterior interventricular branch of left coronary artery were used.
  
  
  RESULTS
  Cilostazol produced concentration-dependent relaxation of calf cardiac vein and coronary artery rings precontracted with carbachol. During cooling, the pIC50 values, but not the maximal responses to cilostazol were significantly lower than at 37 °C in both preparations. Cooling to 28 °C in the presence of NG-nitro-L-arginine methyl ester (L-NAME, 10-4 M) did not modify the effect of temperature both in cardiac vein and coronary artery. These results demonstrate for the first time that cooling-induced changes of cilostazol in calf cardiac vein and coronary artery are independent of NO (Tab. 2, Fig. 3, Ref. 32).
authors.forename: K E
authors.initials: KE
authors.lastname: Nurullahoglu-Atalik
authors.forename: Z U
authors.initials: ZU
authors.lastname: Nurullahoglu
authors.forename: M
authors.initials: M
authors.lastname: Kilic
authors.forename: A
authors.initials: A
authors.lastname: Aribas
mesh_terms: D000818:Animals; D002217:Carbachol; D002417:Cattle; D000077407:Cilostazol; D003080:Cold Temperature; D003331:Coronary Vessels / Q000187:drug effects* / Q000502:physiology*; D066298:In Vitro Techniques; D009126:Muscle Relaxation / Q000187:drug effects*; D009569:Nitric Oxide / Q000502:physiology; D058987:Phosphodiesterase 3 Inhibitors / Q000494:pharmacology*; D013777:Tetrazoles / Q000494:pharmacology*
chemical_list: D058987:Phosphodiesterase 3 Inhibitors; D013777:Tetrazoles; D009569:Nitric Oxide; D002217:Carbachol; D000077407:Cilostazol
keywords: cardiac vein, cilostazol, cooling, coronary artery, nitric oxide.