title: Genetic Variations of Tumor Necrosis Factor -α-308 and Lymphtoxin-α+252 in Non-Hodgkin Lymphoma and Acute Lymphoblastic Leukemia Patients.
abstract: OBJECTIVE
  Non- Hodgkin lymphoma (NHL) and acute lymphoblastic leukemia (ALL) are two main hematological malignances which have been driven from lymphoid tissue. Genetic polymorphisms in tumor necrosis factor-α (TNF-α) -308 and lymphotoxin-α (LT-α) +252 may affect their transcription and expression which leads to their high plasma level. The frequency of the TNF-α (-308) and LT-α (+ 252) polymorphisms are different for NHL and ALL cases in various populations with different ethnicity. This research is designed to investigate the prevalence and association of TNF-α (-308) and LT-α (+ 252) polymorphisms from NHL and ALL in Azarian patients and healthy individuals from Northwestern part of Iran.
  
  
  METHODS
  Seventy subjects with ALL and 68 NHL, along with another 130 healthy subjects as control group took part in this study. Genomic DNA was extracted, then genetic polymorphisms in TNF-α and LT-α genes were analyzed with the PCR-RFLP and NCOI as restriction enzyme. A statistical analysis was performed by chi-square test using SPSS software. A P-value of <0.05 was considered statistically significant.
  
  
  RESULTS
  A statistically significant difference of LT-α polymorphism was in NHL patients and control (P-value= 0.008) but there was not any association of TNF-α polymorphism between NHL patients and control group. A significant association for TNF-a variant was in ALL and control (P-value =0.005), however, there was no relationship about LT variant between ALL and control.
  
  
  CONCLUSIONS
  The results show that there are significant differences between TNF-α (-308) and LT-α (+252) genetic polymorphisms respectively in ALL and NHL patients with control group from Northwestern part of Iran.
authors.affiliation: School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Hajar
authors.initials: H
authors.lastname: Nasiri
authors.forename: Safar
authors.initials: S
authors.lastname: Farajnia
authors.forename: Azim
authors.initials: A
authors.lastname: Rezamand
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Movassaghpour
authors.forename: Heydar Ali
authors.initials: HA
authors.lastname: Esmaeili
authors.forename: Amir
authors.initials: A
authors.lastname: Monfaredan
authors.forename: Naser
authors.initials: N
authors.lastname: Mobarra
authors.forename: Nasser
authors.initials: N
authors.lastname: Rahimifar
authors.forename: Leyla
authors.initials: L
authors.lastname: Sahebi
authors.forename: Majid
authors.initials: M
authors.lastname: Farshdousti Hagh
keywords: Acute lymphocytic leuke- mia; LT-α; Non-Hodgkin lymphoma; Polymorphism; TNF-α