title: Isolation of zebrafish gonads for RNA isolation.
abstract: Piwi proteins and piRNAs are abundant in the gonads of various animal species. Gonads from different developmental stages provide us information regarding the function of piRNAs and the PIWI pathway during germline development. Here we describe methods for gonad and germ cell preparation from different developmental stages of zebrafish. We also describe how to use these gonads to purify and characterize piRNAs.
authors.affiliation: Ontario Cancer Institute, Princess Margaret Hospital, Toronto, ON, Canada.
authors.forename: Hsin-Yi
authors.initials: HY
authors.lastname: Huang
authors.forename: René F
authors.initials: RF
authors.lastname: Ketting
mesh_terms: D000818:Animals; D004210:Dissection / Q000379:methods*; D005260:Female; D005434:Flow Cytometry; D005838:Genotype; D047468:Immunoprecipitation; D008297:Male; D010053:Ovary / Q000166:cytology / Q000378:metabolism*; D012313:RNA / Q000302:isolation & purification*; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D015027:Zebrafish / Q000033:anatomy & histology / Q000196:embryology* / Q000235:genetics*
chemical_list: D012313:RNA