title: The effects of dietary thiamin on oxidative damage and antioxidant defence of juvenile fish.
abstract: The present study explored the effects of thiamin on antioxidant capacity of juvenile Jian carp (Cyprinus carpio var. Jian). In a 60-day feeding trial, a total of 1,050 juvenile Jian carp (8.20 ± 0.02 g) were fed graded levels of thiamin at 0.25, 0.48, 0.79, 1.06, 1.37, 1.63 and 2.65 mg thiamin kg(-1) diets. The results showed that malondialdehyde and protein carbonyl contents in serum, hepatopancreas, intestine and muscle were significantly decreased with increasing dietary thiamin levels (P < 0.05). Conversely, the anti-superoxide anion capacity and anti-hydroxyl radical capacity in serum, hepatopancreas, intestine and muscle were the lowest in fish fed the thiamin-unsupplemented diet. Meanwhile, the activities of catalase (CAT), glutathione peroxidase, glutathione S-transferase and glutathione reductase, and the contents of glutathione in serum, hepatopancreas, intestine and muscle were enhanced with increasing dietary thiamin levels (P < 0.05). Superoxide dismutase (SOD) activity in serum, hepatopancreas and intestine followed a similar trend as CAT (P < 0.05). However, SOD activity in muscle was not affected by dietary thiamin level (P > 0.05). The results indicated that thiamin could improve antioxidant defence and inhibit lipid peroxidation and protein oxidation of juvenile Jian carp.
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Xue-Yin
authors.initials: XY
authors.lastname: Li
authors.forename: Hui-Hua
authors.initials: HH
authors.lastname: Huang
authors.forename: Kai
authors.initials: K
authors.lastname: Hu
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Wei-Dan
authors.initials: WD
authors.lastname: Jiang
authors.forename: Jun
authors.initials: J
authors.lastname: Jiang
authors.forename: Shu-Hong
authors.initials: SH
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Feng
authors.forename: Xiao-Qiu
authors.initials: XQ
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002347:Carps / Q000254:growth & development / Q000378:metabolism*; D002374:Catalase / Q000097:blood; D029941:Fish Proteins / Q000378:metabolism; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D005982:Glutathione Transferase / Q000097:blood; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D008315:Malondialdehyde / Q000097:blood; D009132:Muscles / Q000378:metabolism*; D050050:Protein Carbonylation; D013482:Superoxide Dismutase / Q000097:blood; D013831:Thiamine / Q000008:administration & dosage* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D029941:Fish Proteins; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione; D013831:Thiamine