title: [Effect of hydrogen sulfide on the expression of CSE, NF-κB, and IL-8 mRNA in GES-1 cells with Helicobacter pylori infection].
abstract: OBJECTIVE
  To investigate the effect of hydrogen sulfide (H2S) on the expression of CSE, NF-κB, and IL-8 mRNA in GES-1 cells with Helicobacter pylori (H. pylori) infection and to explore its mechanism on gastric mucosa inflammation caused by H. pylori.
  
  
  METHODS
  GES-1 cells were cultured for 24 h and divided into a control group (neither H. pylori nor NaHS), an H. pylori group, a NaHS group (which was further divided into 4 groups at 50, 100, 200, or 400 μmol/L NaHS), and H. pylori + NaHS group (which was further divided into 4 groups at 50, 100, 200, or 400 μmol/L NaHS). Each group was then cultured for 3, 6, or 12 h. The expression of CSE, NF-κB, and IL-8 mRNA was measured by RT-PCR, and their correlation was analyzed.
  
  
  RESULTS
  The expression of CSE, NF-κB, and IL-8 mRNA in GES-1 cells in the H. pylori group was higher than that in the control group. The expression of CSE in the 200 μmol/L NaHS group and 400 μmol/L NaHS group was lower than that of the control group (P<0.05), whereas the expression of NF-κB and IL-8 in all NaHS groups had no statistical differences compared with the control group (P>0.05). The expression of CSE, NF-κB, and IL-8 mRNA in all groups of NaHS, H. pylori + 200 μmol/L NaHS group, and H. pylori + 400 μmol/L NaHS group was lower than that in the H. pylori group (P<0.05). There was positive correlation among the expressions of CSE, NF-κB, and IL-8 mRNA in the H. pylori group, the H. pylori + 200 μmol/L NaHS group, and the H. pylori + 400 μmol/L NaHS group (P<0.05).
  
  
  CONCLUSIONS
  H. pylori can induce NF-κB and IL-8 mRNA expression and upregulate CSE mRNA expression. At 200 and 400 μmol/L, NaHS can suppress H. pylori-induced NF-κB and IL-8 mRNA expression and ameliorate the morphology of H. pylori-induced GES-1 injury, which may protect gastric epithelial cells by H. pylori infection.
authors.affiliation: Department of Gastroenterology, Third Xiangya Hospital, Central South University, Changsha 410013,China xucanxia2000@hotmail.com.
authors.forename: Canxia
authors.initials: C
authors.lastname: Xu
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Wan
authors.forename: Tao
authors.initials: T
authors.lastname: Guo
authors.forename: Xiong
authors.initials: X
authors.lastname: Chen
mesh_terms: D002460:Cell Line; D003542:Cystathionine gamma-Lyase / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism* / Q000382:microbiology*; D005756:Gastritis; D016481:Helicobacter Infections; D016480:Helicobacter pylori; D006801:Humans; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D016209:Interleukin-8 / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D012333:RNA, Messenger; D013440:Sulfides
chemical_list: D016209:Interleukin-8; D016328:NF-kappa B; D012333:RNA, Messenger; D013440:Sulfides; D003542:Cystathionine gamma-Lyase; C025451:sodium bisulfide; D006862:Hydrogen Sulfide