title: Complete Genome Sequence of a UL96 Mutant Cytomegalovirus Towne-BAC (Bacterial Artificial Chromosome) Isolate Passaged in Fibroblasts To Allow Accumulation of Compensatory Mutations.
abstract: Here, we present the complete genome sequence of a cytomegalovirus Towne-BAC (bacterial artificial chromosome) isolate that we first genetically engineered to mutate the UL96 gene and then serially passaged in human fibroblasts to allow for the accumulation of compensatory mutations. A total of 17 single-base substitutions were discovered in the passaged genome compared to the reference sequence (KF493877).
authors.affiliation: Department of Microbiology, University of Mississippi Medical Center, Jackson, Mississippi, USA.
authors.forename: Teal M
authors.initials: TM
authors.lastname: Brechtel
authors.forename: Molly
authors.initials: M
authors.lastname: Tyner
authors.forename: Ritesh
authors.initials: R
authors.lastname: Tandon