title: Stereospecific biosynthesis of β-methyltryptophan from (L)-tryptophan features a stereochemical switch.
abstract: Make the switch: The three-enzyme cassette MarG/H/I is responsible for stereospecific biosynthesis of β-methyltryptophan from L-tryptophan (1). MarG/I convert 1 into (2S,3R)-β-methyltryptophan, while MarG/I combined with MarH convert 1 into (2S,3S)-β-methyltryptophan. MarH serves as a stereochemical switch by catalyzing the stereoinversion of the β-stereocenter.
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240 (P.R. China).
authors.forename: Yi
authors.initials: Y
authors.lastname: Zou
authors.forename: Qi
authors.initials: Q
authors.lastname: Fang
authors.forename: Haixing
authors.initials: H
authors.lastname: Yin
authors.forename: Zutao
authors.initials: Z
authors.lastname: Liang
authors.forename: Dekun
authors.initials: D
authors.lastname: Kong
authors.forename: Linquan
authors.initials: L
authors.lastname: Bai
authors.forename: Zixin
authors.initials: Z
authors.lastname: Deng
authors.forename: Shuangjun
authors.initials: S
authors.lastname: Lin
mesh_terms: D019998:Racemases and Epimerases / Q000737:chemistry / Q000378:metabolism; D013237:Stereoisomerism; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism*
chemical_list: C538978:beta-methyltryptophan; D014364:Tryptophan; D019998:Racemases and Epimerases
keywords: biosynthesis; cupin proteins; epimerases; reaction mechanism; β-methylamino acids