title: Toxicity of cadmium on Sertoli cell functional competence: an in vitro study.
abstract: Cadmium (Cd), an ubiquitous environmental metal, mainly used for industrial purposes, may be toxic at level of the reproductive system. Testis tubular-based Sertoli cells (SC), play a major role in constituting the blood-testis barrier and provide a unique microenvironment for the genesis and differentiation of germ cells. Hence SC strictly control sperm qualitative and quantitative parameters. We aimed to assess whether exposure to Cd would adversely affect superior mammal SC viability and function. We isolated and purified SC from pre-pubertal pig testes according to our method and incubated the retrieved cells with three different Cadmium chloride concentrations (5-10-15 microM). Parameters of SC function such as inhibin B and anti-Mullerian hormone (AMH) were depressed by Cd exposure, contrary to what observed in untreated controls. No impairment of the FSH receptor integrity on the SC, as assessed by 17-beta-estradiol production, upon stimulation with FSH, was observed in either 5 microM Cd-treated or untreated controls. Differences, on the contrary, were observed for higher Cd concentrations (10 and 15 mM), in terms of FSH receptor integrity, that was altered, as compared to untreated controls, in terms of lower production of 17-beta-estradiol. In addition, the apoptotic test showed a significant increase of early (ANNEXIN V-/Propidium Iodide+) (AV-/PI+) and late apoptotic cells (AV+/ PI+) in all Cd -treated SC conditions as compared to controls. In conclusion, the Cd -related toxicity on SC, clearly demonstrated by our study, even at low concentrations, is expected to damage spermatogenesis that directly is dependent upon retention of SC viability and function.
authors.affiliation: Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Perugia, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Luca
authors.forename: C
authors.initials: C
authors.lastname: Lilli
authors.forename: C
authors.initials: C
authors.lastname: Bellucci
authors.forename: F
authors.initials: F
authors.lastname: Mancuso
authors.forename: M
authors.initials: M
authors.lastname: Calvitti
authors.forename: I
authors.initials: I
authors.lastname: Arato
authors.forename: G
authors.initials: G
authors.lastname: Falabella
authors.forename: S
authors.initials: S
authors.lastname: Giovagnoli
authors.forename: M C
authors.initials: MC
authors.lastname: Aglietti
authors.forename: A
authors.initials: A
authors.lastname: Lumare
authors.forename: G
authors.initials: G
authors.lastname: Muzi
authors.forename: R
authors.initials: R
authors.lastname: Calafiore
authors.forename: M
authors.initials: M
authors.lastname: Bodo
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D002104:Cadmium / Q000493:pharmacokinetics / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D007265:Inhibins / Q000378:metabolism; D008297:Male; D011962:Receptors, FSH / Q000187:drug effects / Q000502:physiology; D012708:Sertoli Cells / Q000187:drug effects* / Q000502:physiology; D013552:Swine
chemical_list: D011962:Receptors, FSH; C422364:inhibin B; D002104:Cadmium; D007265:Inhibins; D054304:Anti-Mullerian Hormone