title: Inhibition of adipogenesis in 3T3-L1 cells and suppression of abdominal fat accumulation in high-fat diet-feeding C57BL/6J mice after downregulation of hyaluronic acid.
abstract: OBJECTIVE
  Adipogenesis can be spatially and temporally regulated by extracellular matrix (ECM). We hypothesized that the regulation of hyaluronic acid (HA), a component of the ECM, can affect adipogenesis in fat cells. The effects of HA on adipogenesis were investigated in vitro in 3T3-L1 cells and in vivo in high-fat diet-feeding C57BL/6J mice.
  
  
  METHODS
  We investigated the effects of HA by degradation of pre-existing or synthesized HA and artificial inhibition of HA synthesis in adipogenesis.
  
  
  RESULTS
  In vitro adipogenesis in 3T3-L1 cells was inhibited by treating them with exogenous hyaluronidase (HYAL) and with 4-methylumbelliferone, which inhibited the synthesis of HA in a concentration-dependent manner. In vivo, abdominal fat accumulation in high-fat diet-feeding C57BL/6J mice was suppressed by exogenous HYAL 10(4) IU injections, which was associated with reduction of lipid accumulation in liver and increase of insulin sensitivity.
  
  
  CONCLUSIONS
  Changes in the ECM such as accumulation of high molecular weight of HA by HAS and degradation of HA by endogenous HYAL were essential for adipogenesis both in vitro and in vivo.
authors.affiliation: Department of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, Korea.
authors.forename: E
authors.initials: E
authors.lastname: Ji
authors.affiliation: Department of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, Korea.
authors.forename: M Y
authors.initials: MY
authors.lastname: Jung
authors.affiliation: Institute of Cancer Research, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors.forename: J H
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, Korea.
authors.forename: S
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, Korea.
authors.forename: C R
authors.initials: CR
authors.lastname: Seo
authors.affiliation: Department of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, Korea.
authors.forename: K W
authors.initials: KW
authors.lastname: Park
authors.affiliation: Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors.forename: E K
authors.initials: EK
authors.lastname: Lee
authors.affiliation: Yeom's Family Medicine Clinic, Seoul, Korea.
authors.forename: C H
authors.initials: CH
authors.lastname: Yeom
authors.affiliation: Department of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, Korea.
authors.forename: S
authors.initials: S
authors.lastname: Lee
mesh_terms: D041721:3T3-L1 Cells / Q000378:metabolism*; D050153:Abdominal Fat / Q000378:metabolism*; D050156:Adipogenesis; D000818:Animals; D002454:Cell Differentiation; D059305:Diet, High-Fat; D015536:Down-Regulation; D005109:Extracellular Matrix / Q000378:metabolism*; D005786:Gene Expression Regulation; D006820:Hyaluronic Acid / Q000378:metabolism*; D007333:Insulin Resistance; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D065626:Non-alcoholic Fatty Liver Disease / Q000503:physiopathology / Q000517:prevention & control; D009765:Obesity / Q000503:physiopathology / Q000517:prevention & control
chemical_list: D006820:Hyaluronic Acid