title: Feasibility of Energy Dispersive X-Ray Fluorescence Determination of Gold in Soft Tissue for Clinical Applications.
abstract: The feasibility of using EDXRF for a rapid quantitative analysis of gold in tumor tissue has been investigated. The protocol described here demonstrates that sample biopsies can be analyzed in as little as 8 hours, with minimal sample preparation. Samples were prepared by drying a 35 μL aliquot of tissue dissolved in KOH in a standard EDXRF cup on a Prolene® support, producing a thin sample. Calibration curves of XRF peak intensity (CPS/mA) to the gold concentration (0-50 PPM) were prepared for liver, tumor, and a mix of tissue types. A linear regression demonstrated an R2correlation of 0.93. Analysis of experimental samples showed that gold accumulation in tumors (5.8-41.3 PPM) was in agreement with previous studies, where samples were processed using INAA or ICP-MS. This report provides guidance for elemental analysis of gold in tissue in a shorter time span, showing potential for its use in a clinical setting.
authors.affiliation: Louisiana Tech University, Department of Biomedical Engineering, PO Box 10157, Ruston, LA, USA. ; Tel: 318 257 4420.
authors.forename: Isidro B
authors.initials: IB
authors.lastname: Magaña
authors.forename: Pratik
authors.initials: P
authors.lastname: Adhikari
authors.forename: Megan C
authors.initials: MC
authors.lastname: Smalley
authors.forename: Sven
authors.initials: S
authors.lastname: Eklund
authors.forename: D Patrick
authors.initials: DP
authors.lastname: O'Neal