title: Fluorescence quenching determination of metallothioneins using 8-hydroxyquinoline-5-sulphonic acid-Cd(II) chelate.
abstract: A novel method for the determination of metallothioneins (MTs) in urine was developed by fluorescence quenching strategy. The response signals linearly correlated with the concentration of MTs in the ranges of 3.12×10(-8)-1.23×10(-6) mol L(-1), and the limit of detection (LOD) was 9.36×10(-9) mol L(-1). The proposed method avoids the label and derivatization steps in common methods, and is reliable, inexpensive and sensitive. Furthermore, the interaction of MTs and 8-hydroxyquinoline-5-sulphonic acid (HQS)-Cd(II) chelate was investigated, and a static quenching mode was proposed to be primarily responsible for the fluorescence quenching event. It could provide a promising potential for the detection of the biomacromolecules which have no native fluorescence, and be benefit to extend the application of fluorescence strategy.
authors.affiliation: College of Public Health, University of South China, Hengyang 421001, PR China.
authors.forename: Qiu-Mei
authors.initials: QM
authors.lastname: Qian
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Jin-Hua
authors.initials: JH
authors.lastname: Xue
authors.forename: Le
authors.initials: L
authors.lastname: Li
authors.forename: Yong-Song
authors.initials: YS
authors.lastname: Wang
authors.forename: Jia-Cheng
authors.initials: JC
authors.lastname: Wang
authors.forename: Ji-Cheng
authors.initials: JC
authors.lastname: Yin
authors.forename: Shan-Du
authors.initials: SD
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D002104:Cadmium / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008668:Metallothionein / Q000032:analysis / Q000652:urine*; D015125:Oxyquinoline / Q000031:analogs & derivatives* / Q000737:chemistry; D011817:Rabbits; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D002614:Chelating Agents; D002104:Cadmium; D015125:Oxyquinoline; C013053:8-hydroxyquinoline-5-sulfonic acid; D008668:Metallothionein
keywords: 8-Hydroxyquinoline-5-sulphonic acid; Cadmium; Fluorescence quenching; Metallothioneins