title: Graphene based pipette tip solid phase extraction of marine toxins in shellfish muscle followed by UPLC-MS/MS analysis.
abstract: Graphene is a novel carbonic material with great potentials for the use as sorbent due to its ultrahigh surface area. Herein, we report the use of graphene as sorbent in solid-phase extraction (SPE) using pipette tip as cartridge namely GPT-SPE, together with ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS), for the analysis of lipophilic marine toxins (LMTs), including yessotoxins (YTX), okadaic acid (OA), dinophysistoxin-1 (DTX1), gymnodimine (GYM), spirolides-1 (SPX1), pectenotoxin-2 (PTX2) and azaspiracid-1 (AZA1) in shellfish. The GPT-SPE procedure was optimized and the performance of graphene was fully validated. Results with high-sensitivity and good reproducibility was obtained and compared with that of other sorbents like C18 silica, multi-walled carbon nanotubes (MWCNTs), commercial Oasis HLB, and Strata-X for the extraction of LMTs, which showed superiority and advantages of graphene, such as good recoveries, stability and compatibility with various solvents. In order to exhibit the potentials of graphene as an excellent sorbent material, 67 mussel samples from six coastal cities of China were analyzed. OA was found to be the dominant contaminant, while YTX was also detected with low level.
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong.
authors.forename: Qing
authors.initials: Q
authors.lastname: Shen
authors.forename: Like
authors.initials: L
authors.lastname: Gong
authors.forename: Joewel T
authors.initials: JT
authors.lastname: Baibado
authors.forename: Wei
authors.initials: W
authors.lastname: Dong
authors.forename: Yixuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Dai
authors.forename: Hon-Yeung
authors.initials: HY
authors.lastname: Cheung
mesh_terms: D000327:Adsorption; D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005663:Furans / Q000302:isolation & purification; D006108:Graphite / Q000737:chemistry*; D006575:Heterocyclic Compounds, 3-Ring / Q000302:isolation & purification; D006844:Hydrocarbons, Cyclic / Q000302:isolation & purification; D007097:Imines / Q000302:isolation & purification; D018942:Macrolides; D008387:Marine Toxins / Q000302:isolation & purification*; D008978:Mollusk Venoms; D009132:Muscles / Q000737:chemistry; D019319:Okadaic Acid / Q000302:isolation & purification / Q000031:analogs & derivatives; D035964:Oxocins / Q000302:isolation & purification; D011714:Pyrans / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012758:Shellfish / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods*; D013141:Spiro Compounds / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry; D000095702:Polyether Toxins
chemical_list: D005663:Furans; D006108:Graphite; D006575:Heterocyclic Compounds, 3-Ring; D006844:Hydrocarbons, Cyclic; D007097:Imines; D018942:Macrolides; D008387:Marine Toxins; D008978:Mollusk Venoms; D019319:Okadaic Acid; D035964:Oxocins; D011714:Pyrans; D013141:Spiro Compounds; C406592:azaspiracid; D000095702:Polyether Toxins; C051904:dinophysistoxin 1; C407350:gymnodimine; C098380:pectenotoxin 2; C066632:yessotoxin
keywords: Graphene; LC–MS/MS; Marine toxins; Pipette tip solid-phase extraction; Shellfish