title: Comparison of four molecular markers in measuring relationships among the wild potato relatives Solanum section Etuberosum (subgenus Potatoe).
abstract: We evaluated chloroplast DNA (cpDNA), isozymes, single to low-copy nuclear DNA (RFLPs), and random amplified polymorphic DNAs (RAPDs) in terms of concordance for genetic distance of 15 accessions each of Solanum etuberosum and S. palustre, and 4 accessions of S. fernandezianum. These self-compatible, diploid (2n=24), and morphologically very similar taxa constitute all species in Solanum sect. Etuberosum, a group of non-tuber-bearing species closely related to Solanum sect. Petota (the potato and its wild relatives). Genetic distance and multidimentional scaling results show general concordance of isozymes, RFLPs and RAPDs between all three taxa; cpDNA shows S. etuberosum and S. palustre to be more similar to each other than to S. fernandezianum. Interspecific sampling variance shows a gradation of resolution from allozyme (low) to RAPD to RFLP (high); while intraspecific comparisons graded from RFLPs (low) to RAPDs (high; lack of sufficient allozyme variability within species precluded comparisons for allozymes). Experimental error was low in RFLPs and RAPDs.
authors.affiliation: Vegetable Crops Research Unit, USDA, Agricultural Research Service, Department of Horticulture, University of Wisconsin, 1575 Linden Drive, 53706-1590, Madison, WI, USA.
authors.forename: D M
authors.initials: DM
authors.lastname: Spooner
authors.forename: J
authors.initials: J
authors.lastname: Tivang
authors.forename: J
authors.initials: J
authors.lastname: Nienhuis
authors.forename: J T
authors.initials: JT
authors.lastname: Miller
authors.forename: D S
authors.initials: DS
authors.lastname: Douches
authors.forename: A
authors.initials: A
authors.lastname: Contreras-M