title: Study of lysozyme resistance in Rhodococcus equi.
abstract: Lysozyme is an important and widespread component of the innate immune response that constitutes the first line of defense against bacterial pathogens. The bactericidal effect of this enzyme relies on its capacity to hydrolyze the bacterial cell wall and also on a nonenzymatic mechanism involving its cationic antimicrobial peptide (CAMP) properties, which leads to membrane permeabilization. In this paper, we report our findings on the lysozyme resistance ability of Rhodococcus equi, a pulmonary pathogen of young foals and, more recently, of immunocompromised patients, whose pathogenic capacity is conferred by a large virulence plasmid. Our results show that (i) R. equi can be considered to be moderately resistant to lysozyme, (ii) the activity of lysozyme largely depends on its muramidase action rather than on its CAMP activity, and (iii) the virulence plasmid confers part of its lysozyme resistance capacity to R. equi. This study is the first one to demonstrate the influence of the virulence plasmid on the stress resistance capacity of R. equi and improves our understanding of the mechanisms enabling R. equi to resist the host defenses.
authors.affiliation: Dozulé Laboratory for Equine Diseases, Bacteriology and Parasitology Unit, ANSES, 14430, Goustranville, France, laurent.hebert@anses.fr.
authors.forename: Laurent
authors.initials: L
authors.lastname: Hébert
authors.forename: Pauline
authors.initials: P
authors.lastname: Bidaud
authors.forename: Didier
authors.initials: D
authors.lastname: Goux
authors.forename: Abdellah
authors.initials: A
authors.lastname: Benachour
authors.forename: Claire
authors.initials: C
authors.lastname: Laugier
authors.forename: Sandrine
authors.initials: S
authors.lastname: Petry
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000378:metabolism*; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial; D009113:Muramidase / Q000378:metabolism*; D010957:Plasmids; D016929:Rhodococcus equi / Q000187:drug effects* / Q000235:genetics
chemical_list: D023181:Antimicrobial Cationic Peptides; D009113:Muramidase