title: Quantitative determination and pharmacokinetic study of aurantio-obtusin in rat plasma by liquid chromatography-mass spectrometry.
abstract: A simple and sensitive liquid chromatography-tandem mass spectrometric method was developed and validated to measure aurantio-obtusin content in rat plasma using rhein as internal standard (IS). The analytes were extracted from plasma by a simple liquid-liquid extraction technique using ethyl acetate as extraction solvent. Chromatographic separation was performed on a Zorbax XDB-C18 column (50 mm × 4.6 mm, 2.1 µm particle size) using the mobile phase consisting of methanol-water (70 : 30, v/v). The detection was achieved by negative ion electrospray ionization in selected reaction monitoring mode, monitoring the transitions m/z 329.1 → 299.2 and m/z 283.1 → 183.0 for aurantio-obtusin and IS, respectively. The assay was linear over a concentration range of 4-4,000 ng/mL with a lower limit of quantification of 4.00 ng/mL for aurantio-obtusin. The intra- and inter-day precision was <6.8%, and the accuracy values were between -1.7 and 7.1%. The validated method was successfully applied to the pharmacokinetics of aurantio-obtusin following intravenous administrations to rats.
authors.affiliation: Department of Emergency Medicine, the First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Emergency Medicine, the First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Dong
authors.affiliation: Department of Emergency Medicine, the First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Li
authors.initials: L
authors.lastname: Pang
authors.affiliation: Department of Gastroenterology, the First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Cardiology, the First Hospital of Jilin University, Changchun 130021, China hongleiji@yeah.net.
authors.forename: Honglei
authors.initials: H
authors.lastname: Ji
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D002138:Calibration; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction / Q000379:methods; D008297:Male; D017208:Rats, Wistar; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000880:Anthraquinones; C000595611:aurantio-obtusin; C020491:rhein