title: Cytotoxic effect of a new endodontic cement and mineral trioxide aggregate on L929 line culture.
abstract: BACKGROUND
  The aim of this study was to compare the cytotoxicity of Mineral Trioxide Aggregate (MTA) and a New Endodontic Cement (NEC) on L929 mouse fibroblasts.
  
  
  METHODS
  Different dilutions (Neat, 1/2, 1/10, 1/100) of fresh and set materials placed adjacent flasks of L929 in DMEM medium. Cellular viability was assessed using MTT assay in three time intervals (24, 48, and 72 h after mixing). Differences in mean cell viability values between materials were assessed by using the One-way ANOVA and Bonferoni post-test. Optical microscopic analysis of morphology of the untreated control and the cement-treated cell cultures were carried out in all experimental periods.
  
  
  RESULTS
  It was indicated that there was not a significant difference in cytotoxicity among the materials of test and between them and the control group. However, there was a statistically significant difference between different time intervals within each group (P< 0.05) and between different concentration of test materials (P<0.05). In all samples, set materials showed better viability than fresh ones.
  
  
  CONCLUSIONS
  According to results of this study, NEC and MTA have similar cytotoxic effect on L929 cell culture.
authors.affiliation: Department of Endodontics, Dental Research Center, Dental School, Mashad University of Medical Sciences, Mashad, Iran and Member of Iranian Center for Endodontic Research.
authors.forename: Jamileh
authors.initials: J
authors.lastname: Ghoddusi
authors.forename: Jalil
authors.initials: J
authors.lastname: Tavakkol Afshari
authors.forename: Zakiyeh
authors.initials: Z
authors.lastname: Donyavi
authors.forename: Azam
authors.initials: A
authors.lastname: Brook
authors.forename: Reza
authors.initials: R
authors.lastname: Disfani
authors.forename: Mohammad
authors.initials: M
authors.lastname: Esmaeelzadeh
keywords: Cytotoxicity test; Fibroblast; Mineral trioxide aggregate; New material