title: Polyphenolic extracts of edible flowers incorporated onto atelocollagen matrices and their effect on cell viability.
abstract: The phenolic extract of chives flowers (Allium schoenoprasum, Liliaceae), introduced Sage (Salvia pratensis, Lamiaceae), European elderberry (Sambucus nigra, Caprifoliaceae) and common dandelion (Taraxacum officinale, Asteraceae) were characterised by High Performance Liquid Chromatography and incorporated in different concentrations onto atelocollagen thin films. In order to assess the biological impact of these phenolic compounds on cell viability, human immortalised non-tumorigenic keratinocyte cell line was seeded on the thin films and cell proliferation was determined by using an MTT assay. In addition, their antimicrobial activity was estimated by using an agar diffusion test. Data indicated the concomitance between cell viability and concentration of polyphenols. These findings suggest that these phenolic-endowed atelocollagen films might be suitable for tissue engineering applications, on account of the combined activity of polyphenols and collagen.
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlín, nám. T.G.Masaryka-5555, Zlín 76001, Czech Republic. humpolicek@ft.utb.cz.
authors.forename: Jorge
authors.initials: J
authors.lastname: López-García
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Kuceková
authors.forename: Petr
authors.initials: P
authors.lastname: Humpolíček
authors.forename: Jiři
authors.initials: J
authors.lastname: Mlček
authors.forename: Petr
authors.initials: P
authors.lastname: Sáha
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry*; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D059808:Polyphenols / Q000737:chemistry* / Q000494:pharmacology*; D023822:Tissue Engineering
chemical_list: D010936:Plant Extracts; D059808:Polyphenols; C059821:atelocollagen; D003094:Collagen