title: Engineered Salmonella typhimurium expressing E7 fusion protein, derived from human papillomavirus, inhibits tumor growth in cervical tumor-bearing mice.
abstract: The tumor-suppressing effects of SipB160/HPV16 E7 fusion protein, derived from human papillomavirus, and expressed in Salmonella enterica serovar typhimurium, were evaluated in a cervical cancer model. The expressed E7 protein resulted in efficacious cytotoxicity and tumor growth retardation in TC-1 cervical cancer cells. In addition, in mice bearing TC-1 tumors, live cells of Salmonella expressing HPV16 E7 were administered orally and induced immune responses through interferon-gamma and tumor necrosis factor-alpha cytokine secretion and also suppressed tumor growth (45 %) and prolonged survival (70 %) compared with the control group. These results suggested that the SipB160/HPV16 E7 fusion protein may be a candidate cancer therapeutic agent.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul, 136-705, Republic of Korea.
authors.forename: Wonsuck
authors.initials: W
authors.lastname: Yoon
authors.forename: Ji Hyeon
authors.initials: JH
authors.lastname: Choi
authors.forename: Sinyeon
authors.initials: S
authors.lastname: Kim
authors.forename: Yong Keun
authors.initials: YK
authors.lastname: Park
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D001688:Biological Products / Q000008:administration & dosage / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D008565:Membrane Proteins / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D060847:Metabolic Engineering; D051379:Mice; D050725:Papillomavirus E7 Proteins / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D012486:Salmonella typhimurium / Q000235:genetics* / Q000378:metabolism*; D016019:Survival Analysis; D002583:Uterine Cervical Neoplasms / Q000628:therapy*
chemical_list: D001426:Bacterial Proteins; D001688:Biological Products; D008565:Membrane Proteins; D050725:Papillomavirus E7 Proteins; D011993:Recombinant Fusion Proteins; C094579:invasion protein B, Salmonella typhimurium