title: Molecular mapping of genes for resistance to rice blast (Pyricularia grisea Sacc.).
abstract: Two dominant genes conferring complete resistance to specific isolates of the rice blast fungus, Pyricularia grisea Sacc., were located on the molecular map of rice in this study. Pi-l(t) is a blast resistance gene derived from the cultivar 'LAC23'. Its map location was determined using a pair of nearly isogenic lines (NILs) and a B6F3 segregating population from which the isoline was derived. RFLP analysis showed that Pi-l(t) is located near the end of chromosome 11, linked to RZ536 at a distance of 14.0±4.5 centiMorgans (cM). A second gene, derived from the cultivar 'Apura', was mapped using a rice doubled-haploid (DH) population. This gene was located on chromosome 12, flanked by RG457 and RG869, at a distance of 13.5+-4.3 cM and 17.7+-4.5 cM, respectively. The newly mapped gene on chromosome 12 may be allelic or closely linked toPi-ta. (=Pi-4(t)), a gene derived from Tetep that was previously reported to be linked to RG869 at a distance of 15.4±4.7 cM. The usefulness of markers linked to blast resistance genes will be discussed in the context of breeding for durable blast resistance.
authors.affiliation: Department of Plant Breeding and Biometry, Cornell University, 14853, Ithaca, NY, USA.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Yu
authors.forename: D J
authors.initials: DJ
authors.lastname: Mackill
authors.forename: J M
authors.initials: JM
authors.lastname: Bonman
authors.forename: S R
authors.initials: SR
authors.lastname: McCouch
authors.forename: E
authors.initials: E
authors.lastname: Guiderdoni
authors.forename: J L
authors.initials: JL
authors.lastname: Notteghem
authors.forename: S D
authors.initials: SD
authors.lastname: Tanksley