title: Decellularized kidney matrix for perfused bone engineering.
abstract: The vascularization of tissue-engineered constructs is yet an unsolved problem. Here, recent work on the decellularization of whole organs has opened new perspectives on tissue engineering. However, existing decellularization protocols last several days and derived biomatrices have only been reseeded with cells from the same tissue origin or stem cells differentiating into these types of tissue. Within the present work, we demonstrate a novel standardized, time-efficient, and reproducible protocol for the decellularization of solid tissues to derive a ready to use biomatrix within only 5 h. Furthermore, we prove that biomatrices are usable as potential scaffolds for tissue engineering of vascularized tissues, even beyond tissue and maybe even species barriers. To prove this, we seeded human primary osteoblasts into a rat kidney bioscaffold. Here, seeded cells spread homogeneously within the matrix and proliferate under dynamic culture conditions. The cells do not only maintain their original phenotype within the matrix, they also show a strong metabolic activity and remodel the biomatrix toward a bone-like extracellular matrix. Thus, the decellularization technique has the ability to become a platform technology for tissue engineering. It potentially offers a universally applicable and easily producible scaffold that addresses the yet unsolved problem of vascularization.
authors.affiliation: 1 Department of Orthopedics, "Klinikum rechts der Isar," Technical University Munich , Munich, Germany .
authors.forename: Rainer
authors.initials: R
authors.lastname: Burgkart
authors.forename: Alexandru
authors.initials: A
authors.lastname: Tron
authors.forename: Peter
authors.initials: P
authors.lastname: Prodinger
authors.forename: Mihaela
authors.initials: M
authors.lastname: Culmes
authors.forename: Jutta
authors.initials: J
authors.lastname: Tuebel
authors.forename: Martijn
authors.initials: M
authors.lastname: van Griensven
authors.forename: Belma
authors.initials: B
authors.lastname: Saldamli
authors.forename: Andreas
authors.initials: A
authors.lastname: Schmitt
mesh_terms: D000818:Animals; D001842:Bone and Bones; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000737:chemistry*; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007668:Kidney / Q000737:chemistry*; D008297:Male; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*