title: The human oligodendrocyte proteome.
abstract: Myelination of the CNS is performed by oligodendrocytes (OLs), which have been implicated in brain disorders, such as multiple sclerosis and schizophrenia. We have used the human oligodendroglial cell line MO3.13 to establish an OL reference proteome database. Proteins were prefractionationated by SDS-PAGE and after in-gel digestion subjected to nanoflow LC-MS analysis. Approximately 11 600 unique peptides were identified and, after stringent filtering, resulted in 2290 proteins representing nine distinct biological processes and various molecular classes and functions. OL-specific proteins, such as myelin basic protein (MBP) and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP), as well as other proteins involved in multiple sclerosis and schizophrenia were also identified and are discussed. Proteins of this dataset have also been classified according to their chromosomal origin for providing useful data to the Chromosome-centric Human Proteome Project (C-HPP). Given the importance of OLs in the etiology of demyelinating and oligodendrogial disorders, the MO3.13 proteome database is a valuable data resource. The MS proteomics data have been deposited to the ProteomeXchange with identifier PXD000263 (http://proteomecentral.proteomexchange.org/dataset/PXD000263).
authors.affiliation: Department of Psychiatry and Psychotherapy, Ludwig Maximilians University of Munich (LMU), Munich, Germany; Research Center for Child Mental Development, University of Fukui, Japan; Department of Development of Functional Brain Activities, United Graduate School of Child Development, Osaka University, Kanazawa University, Hamamatsu University School of Medicine, Chiba University and University of Fukui, Fukui, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Iwata
authors.forename: Cecilia C
authors.initials: CC
authors.lastname: Café-Mendes
authors.forename: Andrea
authors.initials: A
authors.lastname: Schmitt
authors.forename: Johann
authors.initials: J
authors.lastname: Steiner
authors.forename: Takayuki
authors.initials: T
authors.lastname: Manabe
authors.forename: Hideo
authors.initials: H
authors.lastname: Matsuzaki
authors.forename: Peter
authors.initials: P
authors.lastname: Falkai
authors.forename: Christoph W
authors.initials: CW
authors.lastname: Turck
authors.forename: Daniel
authors.initials: D
authors.lastname: Martins-de-Souza
mesh_terms: D002460:Cell Line; D030562:Databases, Protein; D006801:Humans; D004676:Myelin Basic Protein / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009836:Oligodendroglia / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D021122:Protein Subunits / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*
chemical_list: D004676:Myelin Basic Protein; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D021122:Protein Subunits; D020543:Proteome
keywords: Biomedicine; Brain; MO3.13; Multiple sclerosis; Oligodendrocytes; Schizophrenia