title: Characterization and genetic mapping of a Photoperiod-sensitive dwarf 1 locus in rice (Oryza sativa L.).
abstract: Plant height is an important agronomic trait for crop architecture and yield. Most known factors determining plant height function in gibberellin or brassinosteroid biosynthesis or signal transduction. Here, we report a japonica rice (Oryza sativa ssp. japonica) dominant dwarf mutant, Photoperiod-sensitive dwarf 1 (Psd1). The Psd1 mutant showed impaired cell division and elongation, and a severe dwarf phenotype under long-day conditions, but nearly normal growth in short-day. The plant height of Psd1 mutant could not be rescued by gibberellin or brassinosteroid treatment. Genetic analysis with R1 and F2 populations determined that Psd1 phenotype was controlled by a single dominant locus. Linkage analysis with 101 tall F2 plants grown in a long-day season, which were derived from a cross between Psd1 and an indica cultivar, located Psd1 locus on chromosome 1. Further fine-mapping with 1017 tall F2 plants determined this locus on an 11.5-kb region. Sequencing analysis of this region detected a mutation site in a gene encoding a putative lipid transfer protein; the mutation produces a truncated C-terminus of the protein. This study establishes the genetic foundation for understanding the molecular mechanisms regulating plant cell division and elongation mediated by interaction between genetic and environmental factors.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, College of Life Sciences, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Riqing
authors.initials: R
authors.lastname: Li
authors.forename: Jixing
authors.initials: J
authors.lastname: Xia
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Xu
authors.forename: Xiucai
authors.initials: X
authors.lastname: Zhao
authors.forename: Yao-Guang
authors.initials: YG
authors.lastname: Liu
authors.forename: Yuanling
authors.initials: Y
authors.lastname: Chen
mesh_terms: D060406:Brassinosteroids / Q000494:pharmacology; D002874:Chromosome Mapping; D008040:Genetic Linkage; D005875:Gibberellins / Q000494:pharmacology; D009154:Mutation; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D017440:Photoperiod; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000502:physiology
chemical_list: D060406:Brassinosteroids; D005875:Gibberellins; D010937:Plant Growth Regulators; D010940:Plant Proteins; C007842:gibberellic acid