title: Human mesenchymal stem cells response to multi-doped silicon-strontium calcium phosphate coatings.
abstract: The search for apatitic calcium phosphate coatings to improve implants osteointegration is, nowadays, preferentially focused in the obtaining of compositions closer to that of the inorganic phase of bone. Silicon and strontium are both present in trace concentrations in natural bone and have been demonstrated, by separate, to significantly improve osteoblastic response on calcium phosphate bioceramics. This work aims the controlled and simultaneous multi-doping of carbonated calcium phosphate coatings with both elements, Si and Sr, by pulsed laser deposition technique and the biological response of human mesenchymal stem cells to them. A complete physicochemical characterization has been also performed to analyze the coatings and significant positive effect was obtained at the osteogenic differentiation of cells, confirming the enormous potential of this multi-doping coating approach.
authors.affiliation: 1New Materials Group, Applied Physics Dpt., Institute of Biomedical Research of Vigo (IBIV), University of Vigo, Spain.
authors.forename: Cosme
authors.initials: C
authors.lastname: Rodríguez-Valencia
authors.forename: Iago
authors.initials: I
authors.lastname: Pereiro
authors.forename: Rogelio P
authors.initials: RP
authors.lastname: Pirraco
authors.forename: Miriam
authors.initials: M
authors.lastname: López-Álvarez
authors.forename: Julia
authors.initials: J
authors.lastname: Serra
authors.forename: Pío
authors.initials: P
authors.lastname: González
authors.forename: Alexandra P
authors.initials: AP
authors.lastname: Marques
authors.forename: Rui L
authors.initials: RL
authors.lastname: Reis
mesh_terms: D002130:Calcium Phosphates; D002516:Ceramics; D020099:Coated Materials, Biocompatible; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D056951:Photoelectron Spectroscopy; D012825:Silicon; D017550:Spectroscopy, Fourier Transform Infrared; D013324:Strontium
chemical_list: D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; C020243:calcium phosphate; D013324:Strontium; D012825:Silicon
keywords: Multi-doped coatings; calcium phosphate; human bone marrow-derived mesenchymal stem cells differentiation; pulsed laser deposition; silicon; strontium