title: Experiment research on inhibition of glioma with sTRAIL in vitro.
abstract: We report that adenovirus mediated TNF-related apoptosis-inducing ligand (TRAIL) influenced the cell growth and cell cycle in the glioma cells in vitro. After being infected with the Ad-sTRAIL, U251 cell growth was inhibited. The expression of sTRAIL was detected using immunofluorescence. The higher rate of apoptosis was demonstrated using short-term microculture tetrazoliun (MTT) assay and flow cytometry. The rate of Ad-sTRAIL-inducing U251 cell apoptosis was increased depending on the dosage and the time. The apoptosis of G0/G1 and S phase cells was more significant than that of the control groups. The growth and proliferation of U251 cell line was inhibited after the infection of Ad-sTRAIL. It is dose- and time dependent.
authors.affiliation: Department of Neurosurgery, The Affiliated Hospital of Medical College, Qingdao University , Shandong Province , P. R. China.
authors.forename: Yihe
authors.initials: Y
authors.lastname: Dou
authors.forename: Yangang
authors.initials: Y
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.forename: Zhaojian
authors.initials: Z
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Sun
authors.forename: Qinghai
authors.initials: Q
authors.lastname: Meng
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D017209:Apoptosis; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D004247:DNA / Q000378:metabolism; D005434:Flow Cytometry; D005822:Genetic Vectors / Q000235:genetics; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics* / Q000378:metabolism*; D013997:Time Factors
chemical_list: D053221:TNF-Related Apoptosis-Inducing Ligand; D004247:DNA
keywords: Gene therapy; TRAIL; apoptosis; cell cycle