title: Novel fluorescent microarray platforms: a case study in neurodegenerative disorders.
abstract: This review focuses on the fluorescent protein microarrays applied to neurodegenerative disorders, a major health problem in our aging society. Biomarker discovery studies and work on new diagnostic tests are both included. Three platforms are described: antibody planar microarrays, comprising an array of well-defined antibodies for the simultaneous capture of biomarkers; antigen planar microarrays in which proteins, peptides or small molecules are immobilized and probed with biological samples to chase specific antibodies; and bead-based array platforms, which are flow cytometry-based methods in which capture molecules are coupled to fluorescent microspheres, facilitating the simultaneous quantification of several analytes.
authors.affiliation: Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare (ICRM), Milano, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Cretich
authors.forename: Laura
authors.initials: L
authors.lastname: Sola
authors.forename: Paola
authors.initials: P
authors.lastname: Gagni
authors.forename: Marcella
authors.initials: M
authors.lastname: Chiari
mesh_terms: D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D019636:Neurodegenerative Diseases / Q000175:diagnosis*; D040081:Protein Array Analysis / Q000379:methods*
chemical_list: D015415:Biomarkers