title: BAG5 regulates PTEN stability in MCF-7 cell line.
abstract: The phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is a tumor-suppressing lipid phosphatase that is frequently absent in breast tumors. Thus, the stability of PTEN is essential for tumor prevention and therapy. The ubiquitin-proteasome pathway has an important role in regulating the functions of PTEN. Specifically, carboxyl terminus Hsp70-interacting protein (CHIP), the E3 ubiquitin ligase of PTEN, can regulate PTEN levels. In this study, we report that BCL-2-associated athanogene 5 (BAG5), a known inhibitor of CHIP activity, reduces the degradation of PTEN and maintains its levels via an ubiquitylation-dependent pathway. BAG5 is identified as an antagonist of cell tumorigenicity.
authors.affiliation: Department of Breast Surgery, QiLu Hospital of Shandong University, China haidongao@163.com.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Ying
authors.forename: Gao
authors.initials: G
authors.lastname: Haiyan
authors.forename: Gao
authors.initials: G
authors.lastname: Haidong
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D002478:Cells, Cultured; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D061986:MCF-7 Cells; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D011517:Prothrombin Time; D010959:Tissue Plasminogen Activator / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C496515:BAG5 protein, human; D017395:Plasminogen Activator Inhibitor 1; D014409:Tumor Necrosis Factor-alpha; D051059:PTEN Phosphohydrolase; D010959:Tissue Plasminogen Activator