title: Effect of four medicinal plants on amyloid-β induced neurotoxicity in SH-SY5Y cells.
abstract: Amyloid-beta peptide (Aβ) is implicated in the pathogenesis of Alzheimer's disease (AD), a neurodegenerative disorder. This study was designed to determine the effect of four medicinal plants used to treat neurodegenerative diseases on Aβ-induced cell death. Cytotoxicity of the ethanol extracts of the plants was determined against SH-SY5Y (human neuroblastoma) cells which were untreated, as well as toxically induced with Aβ, using the MTT and neutral red uptake assays. Cell viability was reduced to 16% when exposed to 20 µM Aβ25-35 for 72 h. The methanol extract of the roots of Ziziphus mucronata Willd., Lannea schweinfurthii (Engl.) Engl. and Terminalia sericea Burch. ex DC., were the least toxic to the SH-SY5Ycells at the highest concentration tested (100 µg/ml). All four plants tested were observed to reduce the effects of Aβ-induced neuronal cell death, indicating that they may contain compounds which may be relevant in the prevention of AD progression.
authors.affiliation: Department of Pharmacology, Faculty of Health Sciences, University of Pretoria, Private Bag X323, Arcadia 0007, South Africa.
authors.forename: Emanuel A
authors.initials: EA
authors.lastname: Adewusi
authors.forename: Gerda
authors.initials: G
authors.lastname: Fouche
authors.forename: Vanessa
authors.initials: V
authors.lastname: Steenkamp
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000009:adverse effects / Q000378:metabolism*; D027926:Anacardiaceae; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D031407:Crinum; D006801:Humans; D009447:Neuroblastoma; D009474:Neurons / Q000187:drug effects; D010446:Peptide Fragments / Q000009:adverse effects / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D010946:Plants, Medicinal; D029766:Terminalia; D031957:Ziziphus
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D010936:Plant Extracts; C074636:amyloid beta-protein (25-35)
keywords: Amyloid-beta peptide; SH-SY5Y cells; medicinal plants; neurodegenerative disorders; neurotoxicity