title: [Development of sample pretreatment approach and technology for peptidome].
abstract: As a branch of proteomics, peptidome has been extensively applied in biomarker discovery, early diagnosis and pharmacy. In the research of peptidome, sample pretreatment plays a vital role. Here, we review the pretreatment approaches and techniques of peptidome, mainly including ultrafiltration, organic solvent precipitation, solid phase extraction and so on. The biggest challenge of the development of peptidome is the large number of high-abundance proteins in the sample. Therefore, there is an urgent need to develop the fast, efficient, high-throughput and automated sample preparation methods and techniques.
authors.affiliation: Institutes of Biomedical Sciences & Department of Chemistry, Fudan University, Shanghai 200032, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Wei
authors.forename: Haojie
authors.initials: H
authors.lastname: Lu
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
authors.forename: Xin
authors.initials: X
authors.lastname: Wu
mesh_terms: D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D052616:Solid Phase Extraction; D014462:Ultrafiltration
chemical_list: D011506:Proteins