title: An automatic enzyme immunoassay based on a chemiluminescent lateral flow immunosensor.
abstract: Microfluidic integrated enzyme immunosorbent assay (EIA) sensors are efficient systems for point-of-care testing (POCT). However, such systems are not only relatively expensive but also require a complicated manufacturing process. Therefore, additional fluidic control systems are required for the implementation of EIAs in a lateral flow immunosensor (LFI) strip sensor. In this study, we describe a novel LFI for EIA, the use of which does not require additional steps such as mechanical fluidic control, washing, or injecting. The key concept relies on a delayed-release effect of chemiluminescence substrates (luminol enhancer and hydrogen peroxide generator) by an asymmetric polysulfone membrane (ASPM). When the ASPM was placed between the nitrocellulose (NC) membrane and the substrate pad, substrates encapsulated in the substrate pad were released after 5.3 ± 0.3 min. Using this delayed-release effect, we designed and implemented the chemiluminescent LFI-based automatic EIA system, which sequentially performed the immunoreaction, pH change, substrate release, hydrogen peroxide generation, and chemiluminescent reaction with only 1 sample injection. In a model study, implementation of the sensor was validated by measuring the high sensitivity C-reactive protein (hs-CRP) level in human serum.
authors.affiliation: School of Physics and Chemistry, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea.
authors.forename: Hyou-Arm
authors.initials: HA
authors.lastname: Joung
authors.forename: Young Kyoung
authors.initials: YK
authors.lastname: Oh
authors.forename: Min-Gon
authors.initials: MG
authors.lastname: Kim
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002097:C-Reactive Protein / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007124:Immunoenzyme Techniques / Q000379:methods; D008163:Luminescent Measurements / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000379:methods; D011108:Polymers / Q000737:chemistry; D013450:Sulfones / Q000737:chemistry
chemical_list: D011108:Polymers; D013450:Sulfones; C017662:polysulfone P 1700; D002097:C-Reactive Protein; D006861:Hydrogen Peroxide
keywords: Asymmetric polysulfone membrane; Automatic enzyme immunoassay; C-reactive protein; Chemiluminescence; Enzyme immunoassay; Lateral flow immunoassay