title: Regulation of cytochrome P450 mRNA expression in primary porcine hepatocytes by selected secondary plant metabolites from chicory (Cichorium intybus L.).
abstract: Chicory (Cichorium intybus) has been shown to induce enzymes of pharmacokinetic relevance (cytochrome P450; CYP). The aim of this study was to investigate the effects of selected secondary plant metabolites with a global extract of chicory root, on the expression of hepatic CYP mRNA (1A2, 2A19, 2C33, 2D25, 2E1 and 3A29), using primary porcine hepatocytes. Of the tested secondary plant metabolites, artemisinin, scoparone, lactucin and esculetin all induced increased expression of specific CYPs, while esculin showed no effect. In contrast, a global extract of chicory root decreased the expression of CYP1A2, 2C33, 2D25 and 3A29 at high concentrations. The results suggest that purified secondary metabolites from chicory affect CYP expression and thereby might affect detoxification in general, and that global extracts of plants can have effects different from individual components.
authors.affiliation: Department of Food Science, Aarhus University, Denmark. Electronic address: Martink.rasmussen@agrsci.dk.
authors.forename: Martin Krøyer
authors.initials: MK
authors.lastname: Rasmussen
authors.forename: Christina Lindgaard
authors.initials: CL
authors.lastname: Klausen
authors.forename: Bo
authors.initials: B
authors.lastname: Ekstrand
mesh_terms: D000818:Animals; D018651:Cichorium intybus / Q000737:chemistry / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D004929:Esculin / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D007783:Lactones / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D010704:Phorbols / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D010936:Plant Extracts / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D064210:Secondary Metabolism; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D013552:Swine
chemical_list: D007783:Lactones; D010704:Phorbols; D010936:Plant Extracts; D012333:RNA, Messenger; D012717:Sesquiterpenes; D004929:Esculin; D003577:Cytochrome P-450 Enzyme System; C516715:lactucin
keywords: Bioactive compounds; Boar taint; Cryopreservation; Detoxification; Gene regulation; Liver metabolism; Primary hepatocytes