title: Gene expression within the extended amygdala of 5 pairs of rat lines selectively bred for high or low ethanol consumption.
abstract: The objectives of this study were to determine innate differences in gene expression in 2 regions of the extended amygdala between 5 different pairs of lines of male rats selectively bred for high or low ethanol consumption: a) alcohol-preferring (P) vs. alcohol-non-preferring (NP) rats, b) high-alcohol-drinking (HAD) vs. low-alcohol-drinking (LAD) rats (replicate line-pairs 1 and 2), c) ALKO alcohol (AA) vs. nonalcohol (ANA) rats, and d) Sardinian alcohol-preferring (sP) vs. Sardinian alcohol-nonpreferring (sNP) rats, and then to determine if these differences are common across the line-pairs. Microarray analysis revealed up to 1772 unique named genes in the nucleus accumbens shell (AcbSh) and 494 unique named genes in the central nucleus of the amygdala (CeA) that significantly differed [False Discovery Rate (FDR) = 0.10; fold-change at least 1.2] in expression between the individual line-pairs. Analysis using Gene Ontology (GO) and Ingenuity Pathways information indicated significant categories and networks in common for up to 3 or 4 line-pairs, but not for all 5 line-pairs. However, there were almost no individual genes in common within these categories and networks. ANOVAs of the combined data for the 5 line-pairs indicated 1014 and 731 significant (p < 0.01) differences in expression of named genes in the AcbSh and CeA, respectively. There were 4-6 individual named genes that significantly differed across up to 3 line-pairs in both regions; only 1 gene (Gsta4 in the CeA) differed in as many as 4 line-pairs. Overall, the findings suggest that a) some biological categories or networks (e.g., cell-to-cell signaling, cellular stress response, cellular organization, etc.) may be in common for subsets of line-pairs within either the AcbSh or CeA, and b) regulation of different genes and/or combinations of multiple biological systems may be contributing to the disparate alcohol drinking behaviors of these line-pairs.
authors.affiliation: Department of Psychiatry, Institute of Psychiatric Research, Indiana University School of Medicine, 791 Union Drive, Indianapolis, IN 46202-4887, USA. Electronic address: wmcbride@iupui.edu.
authors.forename: William J
authors.initials: WJ
authors.lastname: McBride
authors.forename: Mark W
authors.initials: MW
authors.lastname: Kimpel
authors.forename: Jeanette N
authors.initials: JN
authors.lastname: McClintick
authors.forename: Zheng-Ming
authors.initials: ZM
authors.lastname: Ding
authors.forename: Petri
authors.initials: P
authors.lastname: Hyytia
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Colombo
authors.forename: Tiebing
authors.initials: T
authors.lastname: Liang
authors.forename: Howard J
authors.initials: HJ
authors.lastname: Edenberg
authors.forename: Lawrence
authors.initials: L
authors.lastname: Lumeng
authors.forename: Richard L
authors.initials: RL
authors.lastname: Bell
mesh_terms: D000428:Alcohol Drinking / Q000235:genetics* / Q000378:metabolism; D000679:Amygdala / Q000378:metabolism*; D000818:Animals; D001947:Breeding; D000431:Ethanol / Q000378:metabolism*; D063990:Gene Ontology; D008297:Male; D009714:Nucleus Accumbens / Q000378:metabolism*; D025341:Principal Component Analysis; D051381:Rats; D015398:Signal Transduction / Q000235:genetics; D059467:Transcriptome
chemical_list: D000431:Ethanol
keywords: Alcohol-preferring; Alko alcohol; Central nucleus of the amygdala; Gene expression; High-alcohol-drinking; Nucleus accumbens; Sardinian alcohol-preferring; Selectively bred rat lines