title: Characterization and expression of cytochrome p450 cDNA (CYP9AT2) in Chironomus riparius fourth instar larvae exposed to multiple xenobiotics.
abstract: We identified and characterized a CYP9 family gene, CrCYP9AT2, from Chironomus riparius, an eco-toxicologically important model organism. The 1978 base pair (bp) length CrCYP9AT2 cDNA has an open reading frame of 1587 bp encoding a putative 528 amino acid protein. There was 267 bp 5' and 123 bp 3' untranslated region with a polyadenylation signal site (AATAAA). The putative heme-binding cysteine at position 471 and the typical p450 signature sequence of 463-FGIGPRNCIG-473 were also present. The CrCYP9AT2 transcript was present in all life stages with the highest expression in larvae. The modulation of CrCYP9AT2 was studied using real-time polymerase chain reaction after 24h exposure to cadmium chloride, benzo(a)pyrene; bisphenol A; nonylphenol; chlorpyrifos and ethinylestradiol. Significant up-regulation of CrCYP9AT2 gene was observed after exposure to Cd, B(a)P and CP. However, CrCYP9AT2 was significantly down-regulated after exposure to BPA, NP and EE.
authors.affiliation: School of Environmental Engineering and Graduate School of Energy and Environmental System Engineering, University of Seoul, 90 Jeonnong-dong, Dongdaemun-gu, Seoul 130-743, Republic of Korea; Department of Applied Biosciences, College of Life and Environmental Sciences, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Prakash M Gopalakrishnan
authors.initials: PM
authors.lastname: Nair
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Park
authors.forename: Jinhee
authors.initials: J
authors.lastname: Choi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002683:Chironomidae / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D017931:DNA Primers; D004785:Environmental Pollutants / Q000633:toxicity*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D015262:Xenobiotics / Q000633:toxicity*
chemical_list: D017931:DNA Primers; D004785:Environmental Pollutants; D012333:RNA, Messenger; D015262:Xenobiotics; D003577:Cytochrome P-450 Enzyme System
keywords: C. riparius; CYP gene; Gene expression; Molecular biomarker