title: Peptide nanofibers with dynamic instability through nonequilibrium biocatalytic assembly.
abstract: We demonstrate the formation of supramolecular peptide nanofibers that display dynamic instability; i.e., they are formed by competing assembly and disassembly reactions, where assembly is favored away from equilibrium. The systems are based on competitive catalytic transacylation and hydrolysis, producing a self-assembling aromatic peptide amphiphile from amino acid precursors that temporarily exceeds the critical gelation concentration, until the competing hydrolytic reaction takes over. Analysis by atomic force microscopy shows consecutive nanofiber formation and shortening. The process results in macroscopically observable temporary hydrogelation, which may be repeated upon refueling the system with further addition of the chemically activated amino acid precursor. Nonequilibrium nanostructures open up opportunities for mimicry of the behavior of dynamic gels found in natural systems and provide components for future adaptive nanotechnologies.
authors.affiliation: WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde , 295 Cathedral Street, Glasgow G1 1XL, U.K.
authors.forename: Sisir
authors.initials: S
authors.lastname: Debnath
authors.forename: Sangita
authors.initials: S
authors.lastname: Roy
authors.forename: Rein V
authors.initials: RV
authors.lastname: Ulijn
mesh_terms: D000215:Acylation; D000596:Amino Acids / Q000737:chemistry; D055162:Biocatalysis; D005782:Gels / Q000737:chemistry*; D006868:Hydrolysis; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods; D010455:Peptides / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D005782:Gels; D010455:Peptides