title: Integrated analysis of dysregulated lncRNA expression in fetal cardiac tissues with ventricular septal defect.
abstract: Ventricular septal defects (VSD) are the most common form of congenital heart disease, which is the leading non-infectious cause of death in children; nevertheless, the exact cause of VSD is not yet fully understood. Long non-coding RNAs (lncRNAs) have been shown to play key roles in various biological processes, such as imprinting control, circuitry controlling pluripotency and differentiation, immune responses and chromosome dynamics. Notably, a growing number of lncRNAs have been implicated in disease etiology, although an association with VSD has not been reported. In the present study, we conducted an integrated analysis of dysregulated lncRNAs, focusing specifically on the identification and characterization of lncRNAs potentially involving in initiation of VSD. Comparison of the transcriptome profiles of cardiac tissues from VSD-affected and normal hearts was performed using a second-generation lncRNA microarray, which covers the vast majority of expressed RefSeq transcripts (29,241 lncRNAs and 30,215 coding transcripts). In total, 880 lncRNAs were upregulated and 628 were downregulated in VSD. Furthermore, our established filtering pipeline indicated an association of two lncRNAs, ENST00000513542 and RP11-473L15.2, with VSD. This dysregulation of the lncRNA profile provides a novel insight into the etiology of VSD and furthermore, illustrates the intricate relationship between coding and ncRNA transcripts in cardiac development. These data may offer a background/reference resource for future functional studies of lncRNAs related to VSD.
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Guixian
authors.initials: G
authors.lastname: Song
authors.forename: Yahui
authors.initials: Y
authors.lastname: Shen
authors.forename: Jingai
authors.initials: J
authors.lastname: Zhu
authors.forename: Hailang
authors.initials: H
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.forename: Ya-Qing
authors.initials: YQ
authors.lastname: Shen
authors.forename: Shasha
authors.initials: S
authors.lastname: Zhu
authors.forename: Xiangqing
authors.initials: X
authors.lastname: Kong
authors.forename: Zhangbin
authors.initials: Z
authors.lastname: Yu
authors.forename: Lingmei
authors.initials: L
authors.lastname: Qian
mesh_terms: D016000:Cluster Analysis; D019295:Computational Biology; D005333:Fetus; D020869:Gene Expression Profiling; D006345:Heart Septal Defects, Ventricular / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D058977:Molecular Sequence Annotation; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D015203:Reproducibility of Results; D015398:Signal Transduction; D059467:Transcriptome
chemical_list: D062085:RNA, Long Noncoding