title: A new family of dispersed repeats from Brassica nigra: characterization and localization.
abstract: The 459-bp HindIII (pBN-4) and the 1732-bp Eco RI (pBNE8) fragments from the Brassica nigra genome were cloned and shown to be members of a dispersed repeat family. Of the three major diploid Brassica species, the repeat pBN-4 was found to be highly specific for the B. nigra genome. The family also hybridized to Sinapis arvensis showing that B. nigra had a closer relationship with the S. arvensis genome than with B. oleracea or B. campestris. The clone pBNE8 showed homology to a number of tRNA species indicating that this family of repeats may have originated from a tRNA sequence. The species-specific 459-bp repeat pBN-4 was localized on the B. nigra chromosomes using monosomic addition lines. In addition to the localization of pBN-4, the chromosomal distribution of two other species-specific repeats, pBN34 and pBNBH35 (reported earlier), was studied. The dispersed repeats pBN-4 and pBNBH35 were found to be present on all of the chromosomes, whereas the tandem repeat pBN34 was localized on two chromosomes.
authors.affiliation: Biotechnology Division, Tata Energy Research Institute, Darbari Seth Block, Habitat Place, Lodhi Road, 110 003, New Delhi, India.
authors.forename: R
authors.initials: R
authors.lastname: Kapila
authors.forename: M S
authors.initials: MS
authors.lastname: Negi
authors.forename: P
authors.initials: P
authors.lastname: This
authors.forename: M
authors.initials: M
authors.lastname: Delseny
authors.forename: P S
authors.initials: PS
authors.lastname: Srivastava
authors.forename: M
authors.initials: M
authors.lastname: Lakshmikumaran