title: HIV-1 accessory proteins: Nef.
abstract: Nef is a multifunctional protein encoded by all primate lentiviruses that modulates cell surface expression of a variety of cellular receptors and increases the infectivity of progeny virons. Here, we describe the use of bicistronic HIV-1 constructs that coexpress Nef and fluorescent proteins via an internal ribosome entry site to quantify Nef-mediated receptor modulation in virally infected cells. We also report how such proviral constructs and indicator cell lines can be used to quantify the effect of Nef on virion infectivity.
authors.affiliation: Institute of Molecular Virology, Ulm University Medical Center, Ulm, Germany.
authors.forename: Anke
authors.initials: A
authors.lastname: Heigele
authors.forename: Daniel
authors.initials: D
authors.lastname: Sauter
authors.forename: Jan
authors.initials: J
authors.lastname: Münch
authors.forename: Frank
authors.initials: F
authors.lastname: Kirchhoff
mesh_terms: D002460:Cell Line; D057809:HEK293 Cells; D015497:HIV-1 / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D011956:Receptors, Cell Surface / Q000378:metabolism; D014771:Virion / Q000502:physiology; D054311:nef Gene Products, Human Immunodeficiency Virus / Q000378:metabolism*
chemical_list: D011956:Receptors, Cell Surface; D054311:nef Gene Products, Human Immunodeficiency Virus; C514837:nef protein, Human immunodeficiency virus 1