title: Rejuvenation of chondrogenic potential in a young stem cell microenvironment.
abstract: Autologous cells suffer from limited cell number and senescence during ex vivo expansion for cartilage repair. Here we found that expansion on extracellular matrix (ECM) deposited by fetal synovium-derived stem cells (SDSCs) (FE) was superior to ECM deposited by adult SDSCs (AE) in promoting cell proliferation and chondrogenic potential. Unique proteins in FE might be responsible for the rejuvenation effect of FE while advantageous proteins in AE might contribute to differentiation more than to proliferation. Compared to AE, the lower elasticity of FE yielded expanded adult SDSCs with lower elasticity which could be responsible for the enhancement of chondrogenic and adipogenic differentiation. MAPK and noncanonical Wnt signals were actively involved in ECM-mediated adult SDSC rejuvenation.
authors.affiliation: Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, West Virginia University, PO Box 9196, One Medical Center Drive, Morgantown, WV 26506, USA; Department of Exercise Physiology, West Virginia University, Morgantown, WV 26506, USA.
authors.forename: Jingting
authors.initials: J
authors.lastname: Li
authors.forename: Kirk C
authors.initials: KC
authors.lastname: Hansen
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chenbo
authors.initials: C
authors.lastname: Dong
authors.forename: Cerasela Z
authors.initials: CZ
authors.lastname: Dinu
authors.forename: Monika
authors.initials: M
authors.lastname: Dzieciatkowska
authors.forename: Ming
authors.initials: M
authors.lastname: Pei
mesh_terms: D017209:Apoptosis / Q000502:physiology; D002452:Cell Count; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation; D020219:Chondrogenesis / Q000502:physiology*; D005109:Extracellular Matrix / Q000737:chemistry; D053686:Fetal Stem Cells / Q000166:cytology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D018384:Oxidative Stress / Q000502:physiology; D020543:Proteome / Q000378:metabolism; D012060:Rejuvenation / Q000502:physiology*; D055153:Stem Cell Niche / Q000502:physiology*; D013583:Synovial Membrane / Q000737:chemistry / Q000166:cytology; D060449:Wnt Signaling Pathway
chemical_list: D020543:Proteome
keywords: Cell senescence; Chondrogenesis; Decellularized stem cell matrix; Fetal stem cell; Monolayer expansion; Synovium-derived stem cell