title: Preparative separation of conjugated linoleic acids (CLAs) from fermented Camellia oleifera Abel cake by β-cyclodextrin (β-CD) encapsulation using pH-zone-refining countercurrent chromatography.
abstract: This paper concentrates on the separation of three conjugated linoleic acid (CLA) isomers (trans-9,trans-11 CLA, trans-10,cis-12 CLA and cis-9,trans-11 CLA) by β-cyclodextrin (β-CD) encapsulation using countercurrent chromatography from Camellia oleifera Abel cake fermented by lactic acid bacteria Lactobacillus sp. LL-ZSDS001. The elution sequence of the CLA isomers, the mixing zones and mechanism of separation are discussed. The separation of 305.9mg of the crude sample yielded three isomeric compounds: 91.3mg of trans-9,trans-11 CLA, 84.1mg of trans-10,cis-12 CLA and 79.7mg of cis-9,trans-11 CLA at high purities of 98%, 94% and 96%, respectively.
authors.affiliation: Institute of Food and Biological Engineering, Zhejiang Gongshang University, 149 Jiaogong Road, Hangzhou, Zhejiang 310035, China. Electronic address: sglmail@163.com.
authors.forename: Guanglei
authors.initials: G
authors.lastname: Song
authors.forename: Xiuping
authors.initials: X
authors.lastname: Li
authors.forename: Jiasu
authors.initials: J
authors.lastname: Du
authors.forename: Jingbo
authors.initials: J
authors.lastname: Wang
mesh_terms: D028244:Camellia / Q000737:chemistry* / Q000378:metabolism / Q000382:microbiology; D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D007778:Lactobacillus / Q000378:metabolism*; D044243:Linoleic Acids, Conjugated / Q000737:chemistry / Q000302:isolation & purification*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D044243:Linoleic Acids, Conjugated; D047392:beta-Cyclodextrins; C031215:betadex
keywords: Camellia oleifera Abel; Conjugated linoleic acid; Separation; pH-Zone-refining high-speed countercurrent chromatography; β-Cyclodextrin encapsulating