title: First clinical isolates of Cronobacter spp. (Enterobacter sakazakii) in Argentina: characterization and subtyping by pulsed-field gel electrophoresis.
abstract: Cronobacter species are opportunistic pathogens associated with severe infections in neonates and immunocompromised infants. From January 2009 through September 2010, two cases of neonatal infections associated with Cronobacter malonaticus and one case associated with Cronobacter sakazakii, two of them fatal, were reported in the same hospital. These are the first clinical isolates of Cronobacter spp. in Argentina. The objective of this work was to characterize and subtype clinical isolates of Cronobacter spp. in neonate patients, as well as to establish the genetic relationship between these isolates and the foodborne isolates previously identified in the country. Pulsed-field gel electrophoresis analysis showed a genetic relationship between the C. malonaticus isolates from two patients. Different results were found when the pulsed-field gel electrophoresis patterns of clinical isolates were compared with those deposited in the National Database of Cronobacter spp.
authors.affiliation: Servicio Enterobacterias, Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas (INEI) - ANLIS «Dr. Carlos G. Malbrán», Ciudad Autónoma de Buenos Aires, Argentina. Centro Nacional de Control de Calidad de Biológicos (CNCCB) - ANLIS «Dr. Carlos G. Malbrán», Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Valeria C
authors.initials: VC
authors.lastname: Asato
authors.forename: Viviana E
authors.initials: VE
authors.lastname: Vilches
authors.forename: María G
authors.initials: MG
authors.lastname: Pineda
authors.forename: Enrique
authors.initials: E
authors.lastname: Casanueva
authors.forename: Alejandro
authors.initials: A
authors.lastname: Cane
authors.forename: Mirian P
authors.initials: MP
authors.lastname: Moroni
authors.forename: Silvina P
authors.initials: SP
authors.lastname: Brengi
authors.forename: Mariana G
authors.initials: MG
authors.lastname: Pichel
mesh_terms: D001118:Argentina; D015373:Bacterial Typing Techniques; D044083:Cronobacter sakazakii / Q000145:classification* / Q000302:isolation & purification*; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male