title: Simultaneous determination of imidacloprid and carbendazim in water samples by ion chromatography with fluorescence detector and post-column photochemical reactor.
abstract: A new analytical method has been developed and validated for the simultaneous determination of pesticides from different classes using ion chromatography-online photochemical derivatisation-fluorescence detector (IC-hv-FD). Fluorimetric detection was performed at λex/λem=332 nm/367 nm for imidacloprid and then detector was set at λex/λem=247 nm/470 nm for carbendazim. The two pesticides imidacloprid and carbendazim were successfully separated isocratically on an IonPac(®) AS11 (250 mm × 4 mm i.d; 13 µm particle size, Dionex) anion-exchange column using 40 mM KOH with 10% (v/v) acetonitrile and pumped at a flow rate of 1.0 mL min(-1). Under the optimized conditions, the limit of detection (LOD, S/N=3) of imidacloprid and carbendazim were 7.8 µg L(-1) and 67 µg L(-1), respectively. The experimental results showed that there was good linearity with a correlation coefficient (r)≥0.9966 over the range of 0.05-10 mg L(-1) for imidacloprid and 0.2-15 mg L(-1) for carbendazim. Good reproducibility with a relative standard deviation (RSD, n=7) less than 4.5%. Finally, the proposed method was applied with satisfactory results to the analysis of these pesticides in ground water, lake water and river water without any pre-treatment of samples. The average spiked recoveries were in the range of 90-104%.
authors.affiliation: Department of Chemistry Xixi Campus, Zhejiang University, Hangzhou 310028, China.
authors.forename: Qamar
authors.initials: Q
authors.lastname: Subhani
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Huang
authors.forename: Zuoyi
authors.initials: Z
authors.lastname: Zhu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000097:Acetonitriles; D001562:Benzimidazoles / Q000302:isolation & purification*; D002138:Calibration; D002219:Carbamates / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D005453:Fluorescence; D005618:Fresh Water / Q000737:chemistry*; D007093:Imidazoles / Q000302:isolation & purification*; D057230:Limit of Detection; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000302:isolation & purification*; D010575:Pesticides / Q000302:isolation & purification*; D015203:Reproducibility of Results; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
chemical_list: D000097:Acetonitriles; D001562:Benzimidazoles; D002219:Carbamates; D007093:Imidazoles; D000073943:Neonicotinoids; D009574:Nitro Compounds; D010575:Pesticides; D014874:Water Pollutants, Chemical; C082359:imidacloprid; C006698:carbendazim; C032159:acetonitrile
keywords: Carbendazim; Imidacloprid; Ion chromatography; Photochemical derivatisation