title: On the biology of Spiruroidea parasites of murine rodents on El Hierro (Canary Islands, Spain) with molecular characterization of Streptopharagus greenbergi Wertheim, 1993.
abstract: This paper reports the role of darkling beetles Pimelia laevigata costipennis and Hegeter amaroides (Tenebrionidae) as intermediate hosts of spiruroid nematodes parasites of the black rat and house mouse of El Hierro (Canary Islands). Larvae of spiruroid species were found in the two tenebrionids (18.1% in P. l. costipennis, 7.8% in H. amaroides), Streptopharagus greenbergi being predominant in both (16.1% and 7.1%, respectively), ahead of Mastophorus muris and Gongylonema type larva. The larval stages of S. greenbergi are described for the first time, and adult worms were obtained experimentally from an infected laboratory rat, allowing the identification of the species. Morphometric measurements of experimental adults match those of adults detected in naturally infected rats on the island. Molecular data for S. greenbergi, and the ITS nucleotide sequence of the genus Streptopharagus are also provided for the first time. After the isolation of S. greenbergi DNA and amplification of the ITS region, the ITS1 of this spirocercid was sequenced and deposited in the GenBank database.
authors.affiliation: Laboratory of Parasitology, Faculty of Pharmacy, University of Barcelona, Avenue Joan XXIII s/n, 08028 Barcelona, Spain. Electronic address: Montoliu@ub.edu.
authors.forename: Isabel
authors.initials: I
authors.lastname: Montoliu
authors.forename: Santiago
authors.initials: S
authors.lastname: Sánchez
authors.forename: Mercedes
authors.initials: M
authors.lastname: Villa
authors.forename: Néstor
authors.initials: N
authors.lastname: Abreu-Acosta
authors.forename: Aarón
authors.initials: A
authors.lastname: Martín-Alonso
authors.forename: Ángela
authors.initials: Á
authors.lastname: Fernández-Álvarez
authors.forename: Pilar
authors.initials: P
authors.lastname: Foronda
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000469:parasitology*; D001517:Coleoptera / Q000469:parasitology*; D004199:Disease Vectors; D005260:Female; D007814:Larva; D008297:Male; D051381:Rats / Q000469:parasitology*; D012376:Rodent Diseases / Q000453:epidemiology / Q000469:parasitology*; D013030:Spain / Q000453:epidemiology; D017205:Spirurida Infections / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D013151:Spiruroidea / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000502:physiology
keywords: Caractérisation moléculaire; Coleoptera Tenebrionidae; Experimental infection; Hôtes intermédiaires; Infestation expérimentale; Intermediate hosts; Molecular characterization.; Nematoda Spiruroidea; Streptopharagus greenbergi