title: Antioxidant effects of ryegrass phenolics in lamb liver and plasma.
abstract: Sixteen lambs were divided into two groups and fed two different diets. Eight lambs were stall-fed with a concentrate-based diet (C), and the remaining eight lambs were allowed to graze on Lolium perenne (G). The antioxidant status was measured in the liver and plasma samples before and after solid-phase extraction (SPE) to probe the antioxidant effects that grass phenolic compounds may have conferred onto the animal tissues. The liver and plasma samples from grass-fed lambs displayed a greater antioxidant capacity than the tissues from C lamb group, but only if samples had not been passed through SPE cartridges. Finally, the feed and animal tissues, which had been purified by SPE, were analysed by liquid chromatography combined with mass spectrometry (LC���MS) to identify phenolic compounds present in L. perenne and to evaluate the results from the antioxidant assays. It would appear that the improvement of the antioxidant capacity of lamb liver and plasma from lambs fed ryegrass was not related to the direct transfer of phenolic compounds from grass to the animal tissues.
authors.affiliation: 1 Dottorato di Ricerca in Scienze delle Produzioni Animali, University of Catania, Via Valdisavoia 5, 95123 Catania, Italy.
authors.forename: P
authors.initials: P
authors.lastname: López-Andrés
authors.affiliation: 2 DISPA - Sezione di Scienze delle Produzioni Animali, University of Catania, via Valdisavoia 5, 95123 Catania, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Luciano
authors.affiliation: 2 DISPA - Sezione di Scienze delle Produzioni Animali, University of Catania, via Valdisavoia 5, 95123 Catania, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Vasta
authors.affiliation: 3BioCentre Facility, University of Reading, RG6 6AS, Reading, UK.
authors.forename: T M
authors.initials: TM
authors.lastname: Gibson
authors.affiliation: 4 Dipartimento di Scienze e Tecnologie Agro-forestali e Ambientali, University of Reggio Calabria, Località Feo di Vito, 89100, Reggio Calabria, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Scerra
authors.affiliation: 2 DISPA - Sezione di Scienze delle Produzioni Animali, University of Catania, via Valdisavoia 5, 95123 Catania, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Biondi
authors.affiliation: 2 DISPA - Sezione di Scienze delle Produzioni Animali, University of Catania, via Valdisavoia 5, 95123 Catania, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Priolo
authors.affiliation: 5 Chemistry & Biochemistry Laboratory, Food Production and Quality Research Division, School of Agriculture, Policy and Development, University of Reading, RG6 6AT, Reading, UK.
authors.forename: I
authors.initials: I
authors.lastname: Mueller-Harvey
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002853:Chromatography, Liquid; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008129:Lolium / Q000737:chemistry*; D013058:Mass Spectrometry; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D012756:Sheep / Q000097:blood / Q000378:metabolism*; D052616:Solid Phase Extraction
chemical_list: D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts