title: Characterization of N-demethyllincosamide methyltransferases LmbJ and CcbJ.
abstract: Chemical diversity: Two SAM-dependent N-methyltransferases-LmbJ from the biosynthesis of the antibiotic lincomycin and CcbJ from celesticetin biosynthesis-have been characterized and compared. Both tested enzymes form multimers and are able to utilize N-demethyllincomycin, the natural substrate of LmbJ, with comparable efficiency.
authors.affiliation: Institute of Microbiology AS CR, Vídeňská 1083, 142 20, Prague 4 (Czech Republic).
authors.forename: Lucie
authors.initials: L
authors.lastname: Najmanová
authors.forename: Eva
authors.initials: E
authors.lastname: Kutejová
authors.forename: Jan
authors.initials: J
authors.lastname: Kadlec
authors.forename: Marek
authors.initials: M
authors.lastname: Polan
authors.forename: Jana
authors.initials: J
authors.lastname: Olšovská
authors.forename: Oldřich
authors.initials: O
authors.lastname: Benada
authors.forename: Jitka
authors.initials: J
authors.lastname: Novotná
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Kameník
authors.forename: Petr
authors.initials: P
authors.lastname: Halada
authors.forename: Jacob
authors.initials: J
authors.lastname: Bauer
authors.forename: Jiří
authors.initials: J
authors.lastname: Janata
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D055162:Biocatalysis; D008034:Lincomycin / Q000096:biosynthesis* / Q000737:chemistry; D055231:Lincosamides / Q000096:biosynthesis* / Q000737:chemistry; D008780:Methyltransferases / Q000737:chemistry / Q000378:metabolism*; D008968:Molecular Conformation; D013379:Substrate Specificity
chemical_list: D000900:Anti-Bacterial Agents; D055231:Lincosamides; C004650:celesticetin A; D008034:Lincomycin; D008780:Methyltransferases
keywords: N-methyltransferases; Streptomyces; antibiotics; lincomycin; natural products