title: Macrophage responses to a Zr-based bulk metallic glass.
abstract: Bulk metallic glasses (BMGs) are considered to be a competitive candidate of biomedical materials, owing to their appealing mechanical properties and high thermal processability. Based on the established biosafety of the Zr-based BMGs, macrophage responses to (Zr₅₅ Al₁₀ Ni₅ Cu₃₀)₉₉ Y₁ (atomic percent) BMG were investigated in the present study, in comparison with Ti-6Al-4V alloy. The adhesion of RAW 264.7 macrophages to both alloys was found to be mediated by protein adsorption. The Zr-based BMG is capable of supporting regular adhesion and proliferation of macrophages, indicating its good biocompatibility, which agrees with previous findings using other mammalian cells. A lower degree of morphological activation was revealed on Zr-based BMG substrates than on Ti-6Al-4V substrates, which is evidenced by smaller spreading areas and less ruffling on cell surfaces. Smaller amount of proinflammatory cytokine, tumor necrosis factor-alpha, was secreted by macrophages cultured on Zr-based BMGs, which further confirms the lower level of inflammation induced by BMG than by Ti alloys.
authors.affiliation: Department of Materials Science and Engineering, The University of Tennessee, Knoxville, Tennessee, 37996-2100.
authors.forename: Lu
authors.initials: L
authors.lastname: Huang
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Peter K
authors.initials: PK
authors.lastname: Liaw
authors.forename: Wei
authors.initials: W
authors.lastname: He
mesh_terms: D000327:Adsorption; D000497:Alloys; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005898:Glass / Q000737:chemistry*; D018836:Inflammation Mediators / Q000378:metabolism; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D051379:Mice; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015040:Zirconium / Q000494:pharmacology*
chemical_list: D000497:Alloys; D018836:Inflammation Mediators; D014409:Tumor Necrosis Factor-alpha; C031462:titanium alloy (TiAl6V4); D015040:Zirconium; D014025:Titanium
keywords: cytokine; inflammation; macrophage; metallic glass; protein adsorption