title: Correlation between nitric oxide synthase activity and reduced glutathione level in human and murine endothelial cells.
abstract: The synthesis of nitric oxide (NO), detected as citrulline production, in human (HUVEC) and murine (tEnd.1) endothelial cells correlated with intracellular GSH. tEnd.1, which exhibited an intracellular GSH level 2.5-fold higher than HUVEC, showed a citrulline production (basally and after ionomycin stimulation) 5-8 times higher than human cells. Ionomycinelicited citrulline synthesis in tEnd.1 cells increased 2.4-fold after loading with GSH, and decreased dose-dependently after GSH depletion. Cell loading with N-(2-mercaptopropionyl)-glycine neither significantly increased citrulline production nor relieved the effect of GSH depletion.
authors.affiliation: Dipartimento di Genetica, Biologia e Chimica Medica - Sezione di Chimica Medica, Via Santena 5/bis, I-10126, Torino, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Ghigo
authors.forename: D
authors.initials: D
authors.lastname: Geromin
authors.forename: C
authors.initials: C
authors.lastname: Franchino
authors.forename: R
authors.initials: R
authors.lastname: Todde
authors.forename: C
authors.initials: C
authors.lastname: Priotto
authors.forename: C
authors.initials: C
authors.lastname: Costamagna
authors.forename: M
authors.initials: M
authors.lastname: Arese
authors.forename: G
authors.initials: G
authors.lastname: Garbarino
authors.forename: G P
authors.initials: GP
authors.lastname: Pescarmona
authors.forename: A
authors.initials: A
authors.lastname: Bosia