title: Effect of PDGF-BB, IGF-I growth factors and their combination carried by liposomes in tooth socket healing.
abstract: This work evaluated the bone-forming potential of the platelet-derived growth factor isoform BB (PDGF-BB), insulin-like growth factor I (IGF-I), and mixed PDGF-BB/IGF-I delivered in liposomes compared with phosphate buffered saline (PBS), in the healing process of rat tooth sockets. One hundred and twelve Wistar rats were randomized into 7 groups of 16 animals each and were evaluated at 3, 7, 14 and 21 days after extraction of the maxillary second molars. The left sockets were treated with PBS (P), empty liposome (L), IGF-I in PBS (IP), IGF-I in liposome (IL), PDGF-BB in PBS (PDP), PDGF-BB in liposome (PDL) and both growth factors (GFs) together within liposomes (PDIL). The right sockets were filled with blood clot (BC). Histological and histomorphometric analyses were used to evaluate the formation of new bone and blood vessels. Immunohistochemistry was performed to evaluate the expression of osteocalcin and vascular endothelial growth factor (VEGF) during bone repair. Data were tested statistically using a Tukey's test according to a Dunn's analysis and Mann-Whitney U test followed by Kruskal-Wallis one-way analysis. Results were considered significant when p<0.05. A significantly higher percentage of bone trabeculae and a higher number of blood vessels were observed in the IL, PDL and PDIL groups (p<0.05). However, these GF-liposome groups had statistically similar results. Immunohistochemical assays first detected osteocalcin and VEGF expression at 3 days followed by a peak at 7 days. Lower immunoreactivity levels were observed in the BC, L, P, IP and PDP groups compared with the IL, PDL and PDIL groups (p<0.05). The results suggest that GFs carried by liposomes, either in isolated or mixed forms, enhanced the healing process in rat tooth sockets. The differential expression of the osteogenic markers VEGF and osteocalcin in the early phases of bone healing support these findings.
authors.affiliation: Laboratory of Oral and Development Biology, Department of Morphology, Biological Sciences Institute, UFMG - Federal University of Minas Gerais, Belo HorizonteMG, Brazil.
authors.forename: Fernando Antônio Mauad de
authors.initials: FA
authors.lastname: Abreu
authors.forename: Cynthia Lopes
authors.initials: CL
authors.lastname: Ferreira
authors.forename: Gerluza Aparecida Borges
authors.initials: GA
authors.lastname: Silva
authors.forename: Camila de Oliveira
authors.initials: Cde O
authors.lastname: Paulo
authors.forename: Melissa Nunes
authors.initials: MN
authors.lastname: Miziara
authors.forename: Frank Ferreira
authors.initials: FF
authors.lastname: Silveira
authors.forename: José Bento
authors.initials: JB
authors.lastname: Alves
mesh_terms: D000818:Animals; D000077214:Becaplermin; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage* / Q000494:pharmacology; D008081:Liposomes; D008297:Male; D020574:Proto-Oncogene Proteins c-sis / Q000008:administration & dosage* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D020390:Tooth Socket / Q000473:pathology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D008081:Liposomes; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D007334:Insulin-Like Growth Factor I