title: Poly(hydroxyethyl methacrylate)-based composite cryogel with embedded macroporous cellulose beads for the separation of human serum immunoglobulin and albumin.
abstract: A novel super-macroporous monolithic composite cryogel was prepared by embedding macroporous cellulose beads into poly(hydroxyethyl methacrylate) cryogel. The cellulose beads were fabricated by using a microchannel liquid-flow focusing and cryopolymerization method, while the composite cryogel was prepared by cryogenic radical polymerization of the hydroxyethyl methacrylate monomer with poly(ethylene glycol) diacrylate as cross-linker together with the cellulose beads. After graft polymerization with (vinylbenzyl)trimethylammonium chloride, the composite cryogel was applied to separate immunoglobulin-G and albumin from human serum. Immunoglobulin-G with a mean purity of 83.2% and albumin with a purity of 98% were obtained, indicating the composite cryogel as a promising chromatographic medium in bioseparation for the isolation of important bioactive proteins like immunoglobulins and albumins.
authors.affiliation: State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou, China.
authors.forename: Jialei
authors.initials: J
authors.lastname: Ye
authors.forename: Junxian
authors.initials: J
authors.lastname: Yun
authors.forename: Dong-Qiang
authors.initials: DQ
authors.lastname: Lin
authors.forename: Linhong
authors.initials: L
authors.lastname: Xu
authors.forename: Harald
authors.initials: H
authors.lastname: Kirsebom
authors.forename: Shaochuan
authors.initials: S
authors.lastname: Shen
authors.forename: Gensheng
authors.initials: G
authors.lastname: Yang
authors.forename: Kejian
authors.initials: K
authors.lastname: Yao
authors.forename: Yi-Xin
authors.initials: YX
authors.lastname: Guan
authors.forename: Shan-Jing
authors.initials: SJ
authors.lastname: Yao
mesh_terms: D002482:Cellulose / Q000737:chemistry*; D059985:Cryogels / Q000737:chemistry*; D006801:Humans; D007136:Immunoglobulins / Q000097:blood / Q000302:isolation & purification*; D008863:Microspheres; D010316:Particle Size; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D016062:Porosity; D012709:Serum Albumin / Q000302:isolation & purification*; D013499:Surface Properties
chemical_list: D059985:Cryogels; D007136:Immunoglobulins; D012709:Serum Albumin; D011102:Polyhydroxyethyl Methacrylate; D002482:Cellulose
keywords: Bioseparation; Cellulose beads; Cryogels; Immunoglobulin-G; Ion-exchange adsorption