title: Investigating the molecular structural features of hulless barley (Hordeum vulgare L.) in relation to metabolic characteristics using synchrotron-based fourier transform infrared microspectroscopy.
abstract: The synchrotron-based Fourier transform infrared microspectroscopy (SR-FTIRM) technique was used to quantify molecular structural features of the four hulless barley lines with altered carbohydrate traits [amylose, 1-40% of dry matter (DM); β-glucan, 5-10% of DM] in relation to rumen degradation kinetics, intestinal nutrient digestion, and predicted protein supply. Spectral features of β-glucan (both area and heights) in hulless barley lines showed a negative correlation with protein availability in the small intestine, including truly digested protein in the small intestine (DVE) (r = -0.76, P < 0.01; r = -0.84, P < 0.01) and total metabolizable protein (MP) (r = -0.71, P < 0.05; r = -0.84, P < 0.01). Variation in absorption intensities of total carbohydrate (CHO) was observed with negative effects on protein degradation, digestion, and potential protein supply (P < 0.05). Molecular structural features of CHO in hulless barley have negative effects on the supply of true protein to ruminants. The results clearly indicated the impact of the carbohydrate-protein structure and matrix.
authors.affiliation: Department of Animal and Poultry Science and ‡Crop Development Centre, College of Agriculture and Bioresources, University of Saskatchewan , 51 Campus Drive, Saskatoon, Saskatchewan S7N 5A8, Canada.
authors.forename: Ling
authors.initials: L
authors.lastname: Yang
authors.forename: David A
authors.initials: DA
authors.lastname: Christensen
authors.forename: John J
authors.initials: JJ
authors.lastname: McKinnon
authors.forename: Aaron D
authors.initials: AD
authors.lastname: Beattie
authors.forename: Hangshu
authors.initials: H
authors.lastname: Xin
authors.forename: Peiqiang
authors.initials: P
authors.lastname: Yu
mesh_terms: D000688:Amylose / Q000378:metabolism; D000821:Animal Feed; D000818:Animals; D002241:Carbohydrates / Q000737:chemistry; D056625:Endosperm / Q000737:chemistry; D001467:Hordeum / Q000737:chemistry* / Q000378:metabolism*; D007700:Kinetics; D010940:Plant Proteins / Q000737:chemistry / Q000493:pharmacokinetics; D012417:Rumen / Q000378:metabolism; D012418:Ruminants / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D017356:Synchrotrons; D047071:beta-Glucans / Q000378:metabolism
chemical_list: D002241:Carbohydrates; D010940:Plant Proteins; D047071:beta-Glucans; D000688:Amylose