title: The effects of ryanodine receptor 1 (RYR1) mutation on plasma cytokines and catecholamines during prolonged restraint in pigs.
abstract: In the current study, plasma cytokines, including interleukin (IL) 1, IL-2, IL-6, IL-10, IL-12, interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) and catecholamines (adrenaline and noradrenaline) were evaluated during 4h restraint and recovery phase in 60 male pigs. Blood samples were collected from three groups of pigs representing different RYR1 genotypes, namely NN homozygotes (wild-type), Nn heterozygous and nn homozygous (mutant). The 4h restraint evoked an increase in plasma cytokine concentrations in each of the RYR1 genotype groups. During the restraint, the greatest concentrations of plasma IL-6, IL-10, IL-12 and TNF-α in nn homozygous pigs and IFN-γ in NN homozygous were observed. Interleukin 1, IL-2, IL-10, and TNF-α measures were positively intercorrelated in each of the three RYR1 genotype group. A positive correlation was seen between all measured cytokines (with the exception of IL-6) and plasma catecholamine concentrations in Nn heterozygous and nn homozygous pigs. The results suggest that of the cytokine parameters evaluated, IL-6, IL-10, IL-12 and TNF-α of the nn homozygous group seem to show a stronger stress-related response as compared with those of the other two (NN and Nn) groups.
authors.affiliation: Department of Animal and Human Physiology, University of Gdansk, 80-308 Gdansk, Poland. Electronic address: zetciep@gazeta.pl.
authors.forename: Ziemowit M
authors.initials: ZM
authors.lastname: Ciepielewski
authors.forename: Wojciech
authors.initials: W
authors.lastname: Stojek
authors.forename: Wojciech
authors.initials: W
authors.lastname: Glac
authors.forename: Dorota
authors.initials: D
authors.lastname: Myślińska
authors.forename: Anna
authors.initials: A
authors.lastname: Kwaczyńska
authors.forename: Marian
authors.initials: M
authors.lastname: Kamyczek
mesh_terms: D000818:Animals; D002395:Catecholamines / Q000097:blood*; D016207:Cytokines / Q000097:blood*; D005838:Genotype; D008297:Male; D009154:Mutation; D012044:Regression Analysis; D012149:Restraint, Physical; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics*; D013552:Swine
chemical_list: D002395:Catecholamines; D016207:Cytokines; D019837:Ryanodine Receptor Calcium Release Channel
keywords: Catecholamines; Cytokines; Pig; RYR1 gene; Restraint stress