title: [Effects of surgical operation on helper-inducer T-lymphocytes in sino-nasal neoplasms patients].
abstract: OBJECTIVE
  To explore the effects of surgical operation on helper-inducer T-lymphocytes (Th1/Th2) in sino-nasal neoplasms.
  
  
  METHODS
  From January 2004 to December 2011, 80 patients with malignant tumor in nasal cavities or sinuses were enrolled as experimental group and another 80 subjects with deflection of nasal septum as control group. The phorbol-12-myristate-13-acetate (PMA)-stimulated peripheral blood mononuclear cells (PBMC) were obtained from both malignant tumor and normal control tissues. And flow cytometry was used to detect the expression percentages of interleukin-4 (IL-4) in Th2 cell and interferon-gamma (IFN-γ) in Th1 cell at pre and post-operation.
  
  
  RESULTS
  The preoperative expression percentage of CD3(+) CD8(-) IL-4(+) cell in malignant tumor patients was higher than that in controls (6.6% ± 1.7% vs 2.8% ± 1.7%, P < 0.05) while the expression percentage of CD3(+) CD8(-) IFN-γ(+) cell was lower (18.7% ± 5.7% vs 59.3% ± 1.5%, P < 0.05). The preoperative expression percentage of CD3(+) CD8(-) IL-4(+) cell was higher than that at postoperation (6.6% ± 1.7 %vs 2.8% ± 1.5%, P < 0.05). And the postoperative expression percentage of CD3(+) CD8(-) IFN-γ(+) cell was higher than that at preoperation (54.0% ± 4.0% vs 18.7% ± 5.7%, P < 0.05).
  
  
  CONCLUSIONS
  Surgery may restore the immune balance in patients with malignant tumors in nasal cavities or sinuses.
authors.affiliation: Department of Otolaryngology, Qingdao Municipal Hospital, Shandong, Qingdao 266011, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.forename: Qin-song
authors.initials: QS
authors.lastname: Liu
authors.forename: Jing-hua
authors.initials: JH
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Yuan
authors.forename: Wei-guo
authors.initials: WG
authors.lastname: Xue
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009669:Nose Neoplasms / Q000378:metabolism / Q000601:surgery*; D010255:Paranasal Sinus Neoplasms / Q000378:metabolism / Q000601:surgery*; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000378:metabolism*; D018417:Th1 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism
chemical_list: C508598:IL4 protein, human; D015847:Interleukin-4; D007371:Interferon-gamma