title: Interactive effects of mechanical ventilation, inhaled nitric oxide and oxidative stress in acute lung injury.
abstract: To compare conventional mechanical ventilation (CMV) and high-frequency oscillatory ventilation (HFOV), with/without inhaled nitric oxide (iNO), for oxygenation, inflammation, antioxidant/oxidative stress status, and DNA damage in a model of acute lung injury (ALI). Lung injury was induced by tracheal infusion of warm saline. Rabbits were ventilated at [Formula: see text] 1.0 and randomly assigned to one of five groups. Overall antioxidant defense/oxidative stress was assessed by total antioxidant performance assay, and DNA damage by comet assay. Ventilatory and hemodynamic parameters were recorded every 30min for 4h. ALI groups showed worse oxygenation than controls after lung injury. After 4h of mechanical ventilation, HFOV groups presented significant improvements in oxygenation. HFOV with and without iNO, and CMV with iNO showed significantly increased antioxidant defense and reduced DNA damage than CMV without iNO. Inhaled nitric oxide did not beneficially affect HFOV in relation to antioxidant defense/oxidative stress and pulmonary DNA damage. Overall, lung injury was reduced using HFOV or CMV with iNO.
authors.affiliation: Department of Pediatrics, Botucatu Medical School - Sao Paulo State University (UNESP), Botucatu, SP, Brazil; Jean Mayer USDA - Human Nutrition Research Center on Aging, Tufts University, Boston, MA, USA; Department of Health Sciences - Universidade do Sagrado Coração (USC), Bauru, SP, Brazil. Electronic address: ronchi.carlos@yahoo.com.
authors.forename: Carlos Fernando
authors.initials: CF
authors.lastname: Ronchi
authors.forename: Ana Lucia Anjos
authors.initials: AL
authors.lastname: Ferreira
authors.forename: Fabio Joly
authors.initials: FJ
authors.lastname: Campos
authors.forename: Cilmery Suemi
authors.initials: CS
authors.lastname: Kurokawa
authors.forename: Mario Ferreira
authors.initials: MF
authors.lastname: Carpi
authors.forename: Marcos Aurélio
authors.initials: MA
authors.lastname: Moraes
authors.forename: Rossano Cesar
authors.initials: RC
authors.lastname: Bonatto
authors.forename: Kyung-Jin
authors.initials: KJ
authors.lastname: Yeum
authors.forename: Jose Roberto
authors.initials: JR
authors.lastname: Fioretto
mesh_terms: D055371:Acute Lung Injury / Q000473:pathology / Q000503:physiopathology* / Q000628:therapy*; D000280:Administration, Inhalation; D000818:Animals; D001993:Bronchodilator Agents / Q000008:administration & dosage*; D002452:Cell Count; D004249:DNA Damage / Q000187:drug effects; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D009566:Nitrates / Q000097:blood; D009569:Nitric Oxide / Q000008:administration & dosage*; D009573:Nitrites / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects*; D011659:Pulmonary Gas Exchange / Q000187:drug effects / Q000502:physiology; D011817:Rabbits; D012121:Respiration, Artificial; D013997:Time Factors
chemical_list: D001993:Bronchodilator Agents; D009566:Nitrates; D009573:Nitrites; D009569:Nitric Oxide
keywords: Conventional mechanical ventilation; DNA damage; High-frequency oscillatory ventilation; Inhaled nitric oxide; Pulmonary oxidative stress