title: [PP2 enhances intercellular communication of gap junction in breast cancer Hs578T cells].
abstract: OBJECTIVE
  To investigate the effect of Src kinase inhibitor PP2 on intercellular communication of gap junction in breast cancer cells.
  
  
  METHODS
  Cultured breast cancer Hs578T cells were treated with various concentrations of pp2 (0,1,2,4,8,16,32 μmol/L) for 24h. Cell growth was determined by MTT assay; dye spread in Hs578T cells was measured by Parachute assay; and the expression of Src kinase in Hs578T cells was detected by Western blot.
  
  
  RESULTS
  MTT assay showed that the survive rate of Hs578T cells treated with PP2 (1 ≊ 8 μmol/L) was 98% ± 3% ≊ 94 % ± 4%. Parachute assay showed that compared to control group the standard normalized dye spread rates of Hs578T cells treated with 1,2,4 and 8 μmol/L PP2 were 1.60 ± 0.08,2.00 ± 0.05,2.20 ± 0.05 and 2.70 ± 0.09,respectively (all P<0.01). Moreover,compared to control group at the same time points,the standard normalized dye spread of Hs578T cells treated with 8 μmol/L PP2 for 6,12 and 24 h were 1.4 ± 0.05,1.7 ± 0.06,and 2.2 ± 0.07,respectively (all P<0.01). Western blot showed that the expression ratios of Src kinase/β-actin of Hs578T cells treated with 1,2,4 and 8 μmol/L PP2 for 24 h were 0.93 ± 0.02,0.70 ± 0.09,0.66 ± 0.09 and 0.36 ± 0.10,which were significantly inhibited compared with control group (P<0.05 or 0.01). And the expression ratio of Src kinase/β-actin of Hs578T cells treated with 8 μmol/L PP2 for 6,12 and 24h was 0.82 ± 0.03,0.66 ± 0.08 and 0.59 ±0.09, which were all inhibited significantly compared to control group (P<0.01).
  
  
  CONCLUSIONS
  PP2 enhances the gap junction function in breast cancer Hs578T cells, which is probably related to the inhibition of Src kinase.
authors.affiliation: Department of Pharmacology,Faculty of Pharmacy,Bengbu Medical College,Bengbu 233000,China.
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Dong
authors.forename: Chao
authors.initials: C
authors.lastname: Zheng
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Jiang
authors.forename: Xi
authors.initials: X
authors.lastname: Han
authors.forename: Xu-Hui
authors.initials: XH
authors.lastname: Tong
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D005260:Female; D017629:Gap Junctions / Q000187:drug effects*; D006801:Humans; D011743:Pyrimidines / Q000008:administration & dosage / Q000494:pharmacology*; D019061:src-Family Kinases / Q000378:metabolism*
chemical_list: C412373:AG 1879; D011743:Pyrimidines; D019061:src-Family Kinases