title: Screening for antiradical efficiency of 21 semi-synthetic derivatives of quercetin in a DPPH assay.
abstract: The group of 21 novel semi-synthetic derivatives of quercetin was screened for the antiradical efficiency in a DPPH assay. The initial fast absorbance decrease of DPPH, corresponding to the transfer of the most labile H atoms, was followed by a much slower absorbance decline representing the residual antiradical activity of the antioxidant degradation products. Initial velocity of DPPH decolorization determined for the first 75-s interval was used as a marker of the antiradical activity. Application of the kinetic parameter allowed good discrimination between the polyphenolic compounds studied. The most efficient chloronaphthoquinone derivative (compound Ia) was characterized by antiradical activity higher than that of quercetin and comparable with that of trolox. Under the experimental conditions used, one molecule of Ia was found to quench 2.6±0.1 DPPH radicals.
authors.affiliation: Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, 84104 Bratislava, Slovak Republic.
authors.forename: Ivana
authors.initials: I
authors.lastname: Milackova
authors.forename: Lucia
authors.initials: L
authors.lastname: Kovacikova
authors.forename: Miroslav
authors.initials: M
authors.lastname: Veverka
authors.forename: Ján
authors.initials: J
authors.lastname: Gallovic
authors.forename: Milan
authors.initials: M
authors.lastname: Stefek
keywords: DPPH assay; antioxidant; kinetics; quercetin derivatives; stoichiometry