title: The anti-tumor effect and biological activities of the extract JMM6 from the stem-barks of the Chinese Juglans mandshurica Maxim on human hepatoma cell line BEL-7402.
abstract: Juglans mandshurica Maxim is a traditional herbal medicines in China, and its anti-tumor bioactivities are of research interest. Bioassay-guided fractionation method was employed to isolate anti-tumor compounds from the stem barks of the Juglans mandshurica Maxim. The anti-tumor effect and biological activities of the extracted compound JMM6 were studied in BEL-7402 cells by MTT, Cell cycle analysis, Hoechst 33342 staining, Annexin V-FITC/PI assay and Detection of mitochondrial membrane potential (ΔΨm). After treatment with the JMM6, the growth of BEL-7402 cells was inhibited and cells displayed typical morphological apoptotic characteristics. Further investigations revealed that treatment with JMM6 mainly caused G2/M cell cycle arrest and induced apoptosis in BEL-7402 cells. To evaluate the alteration of mitochondria in JMM6 induced apoptosis. The data showed that JMM6 decreased significantly the ΔΨm, causing the depolarization of the mitochondrial membrane. Our results show that the JMM6 will have a potential advantage of anti-tumor, less harmful to normal cells. This paper not only summarized the JMM6 pick-up technology from Juglans mandshurica Maxim and biological characteristic, but also may provide further evidence to exploit the potential medicine compounds from the stem-barks of the Chinese Juglans mandshurica Maxim.
authors.affiliation: Department of Biology, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Cui
authors.forename: Jiayong
authors.initials: J
authors.lastname: Zhu
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Li
authors.forename: Jianwen
authors.initials: J
authors.lastname: Mao
authors.forename: Xiaobao
authors.initials: X
authors.lastname: Jin
authors.forename: Xiangsheng
authors.initials: X
authors.lastname: Wang
authors.forename: Yifan
authors.initials: Y
authors.lastname: Du
authors.forename: Jiazheng
authors.initials: J
authors.lastname: Lu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000378:metabolism; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D031324:Juglans; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008517:Phytotherapy; D024301:Plant Bark; D018547:Plant Stems
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal
keywords: Juglans mandshurica Maxim; anti-tumor; apoptosis; cell cycle; mitochondrial membrane potential; stem-barks