title: Fluorogenic probes using 4-substituted-2-nitrobenzenesulfonyl derivatives as caging groups for the analysis of human glutathione transferase catalyzed reactions.
abstract: We have synthesized a series of 4-substituted-2-nitrobenzene-sulfonyl compounds for caged fluorogenic probes and conducted a Hammett plot analysis using the steady-state kinetic parameters. The results revealed that the glutathione transferase (GST) alpha catalyzed reaction was dependent on the σ value in the same way as the non-enzymatic reaction, whereas the dependence of the σ value of the GST mu and pi was not as pronounced as that of GST alpha.
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako-Shi, Saitama 351-0198, Japan. y-ito@riken.jp.
authors.forename: Aya
authors.initials: A
authors.lastname: Shibata
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Nakano
authors.forename: Mika
authors.initials: M
authors.lastname: Ito
authors.forename: Mika
authors.initials: M
authors.lastname: Araki
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Satoshi
authors.initials: S
authors.lastname: Shuto
authors.forename: Bengt
authors.initials: B
authors.lastname: Mannervik
authors.forename: Ralf
authors.initials: R
authors.lastname: Morgenstern
authors.forename: Ralf
authors.initials: R
authors.lastname: Mogenstern
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Abe
mesh_terms: D055162:Biocatalysis; D005456:Fluorescent Dyes / Q000737:chemistry*; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D009578:Nitrobenzenes / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D009578:Nitrobenzenes; C036077:nitrobenzene; D005982:Glutathione Transferase