title: Safety profile of antimicrobial peptides: camel, citropin, protegrin, temporin a and lipopeptide on HaCaT keratinocytes.
abstract: Antimicrobial peptides (AMPs) are an essential part of the innate immunity of the skin and mucosal surfaces. They have a broad spectrum of antimicrobial activity: antibacterial, antifungal, antiviral as well as antiprotozoal. Numerous studies using AMPs as potential agents against different microbes has been performed during the last two decades. Here we investigated antistaphylococcal activity and safety of following AMPs: camel, citropin, protegrin, temporin A and lipopeptide Palm-KK-NH2. The susceptibility of the strains of Staphylococcus aureus isolated from the patients with erythrodermia to conventional antibiotics and AMPs was determined by the broth dilution method. The cytotoxicity assay was performed on HaCaT keratinocytes. Tested peptides turned out to be very effective against all clinical isolates, including strains resistant to conventional antibiotics. The majority of the examined peptides as well as conventional antimicrobials do not exert any toxic effect on HaCaT cells in minimal inhibitory concentration. Peptides are very promising agents for the topical treatment of staphylococcal skin infections. The most promising seem to be citropin 1.1 and temporin A, as they were toxic only in two highest concentration (50 and 100 microg/mL), with relatively low MIC values.
authors.affiliation: Department of Dermatology, Venereology and Allergology, Faculty of Medicine, Medical University of Gdansk, Debinki 7, 80-211 Gdańsk, Poland.
authors.forename: Wioletta
authors.initials: W
authors.lastname: Barańska-Rybak
authors.forename: Michał
authors.initials: M
authors.lastname: Pikula
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Dawgul
authors.forename: Wojciech
authors.initials: W
authors.lastname: Kamysz
authors.forename: Piotr
authors.initials: P
authors.lastname: Trzonkowski
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Roszkiewicz
mesh_terms: D000890:Anti-Infective Agents / Q000633:toxicity*; D023181:Antimicrobial Cationic Peptides / Q000633:toxicity*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D011506:Proteins / Q000633:toxicity; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D011506:Proteins; C104501:temporin