title: Coating with autologous plasma improves biocompatibility of mesh grafts in vitro: development stage of a surgical innovation.
abstract: OBJECTIVE
  To investigate mesh coating modalities with autologous blood components in a recently developed in vitro test system for biocompatibility assessment of alloplastic materials.
  
  
  METHODS
  Seven different mesh types, currently used in various indications, were randomly investigated. Meshes were coated prior to cultivation with autologous peripheral blood mononuclear cells (PBMCs), platelets, and blood plasma. Pretreated meshes were incubated over 6 weeks in a minced tissue assay, representative for fibroblasts, muscle cells, and endothelial cells originating from 10 different patients. Adherence of those tissues on the meshes was microscopically investigated and semiquantitatively assessed using a previously described scoring system.
  
  
  RESULTS
  Coating with peripheral blood mononuclear cells did not affect the adherence score, whereas coating with platelets and blood plasma increased the score suggesting improved biocompatibility in vitro. The previous ranking of native meshes remained consistent after coating.
  
  
  CONCLUSIONS
  Plasma coating of meshes improves their biocompatibility score in a novel in vitro test system.
authors.affiliation: Department of Urology, Lukas Hospital, Preußenstraße 84, 41464 Neuss, Germany ; West German Cancer Center (WTZ), University of Essen, Essen, Germany ; German Centre for Assessment and Evaluation of Innovative Techniques in Medicine (DZITM), Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Gerullis
authors.forename: Evangelos
authors.initials: E
authors.lastname: Georgas
authors.forename: Christoph
authors.initials: C
authors.lastname: Eimer
authors.forename: Christian
authors.initials: C
authors.lastname: Arndt
authors.forename: Dimitri
authors.initials: D
authors.lastname: Barski
authors.forename: Bernhard
authors.initials: B
authors.lastname: Lammers
authors.forename: Bernd
authors.initials: B
authors.lastname: Klosterhalfen
authors.forename: Mihaly
authors.initials: M
authors.lastname: Borós
authors.forename: Thomas
authors.initials: T
authors.lastname: Otto
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D005347:Fibroblasts / Q000737:chemistry / Q000187:drug effects; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D019736:Prostheses and Implants; D013526:Surgical Mesh
chemical_list: D001672:Biocompatible Materials