title: CGmCGCG is a versatile substrate with which to evaluate Tet protein activity.
abstract: Tet family proteins have the ability to convert 5-methylcytosine (mC) to 5-hydroxymethylcytosine, and further to 5-formylcytosine and 5-carboxycytosine. We found that CGmCGCG can be the substrate of Tet protein, and observed iterative oxidation of mC by HPLC analysis. We also demonstrated that Tet protein favours single-stranded DNA over double-stranded DNA.
authors.affiliation: Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan. hs@kuchem.kyoto-u.ac.jp.
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Kizaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiyama
mesh_terms: D044503:5-Methylcytosine / Q000737:chemistry / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D009843:Oligoribonucleotides / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction
chemical_list: D004268:DNA-Binding Proteins; D009843:Oligoribonucleotides; D044503:5-Methylcytosine