title: A fast and cost-effective method for identifying a polymorphism of interleukin 28B related to hepatitis C.
abstract: Approximately 170 million people are chronic carriers of hepatitis C virus (HCV). Patients with chronic hepatitis C are currently treated with pegylated interferon and ribavirin (PEG-IFN/RBV). A genome-wide association with PEG-IFN/RBV treatment response and a single nucleotide polymorphism (rs12979860) has been identified near the interleukin 28B gene that encodes interferon-λ-3. In this paper, we describe an innovative, fast, and low-cost multiplex polymerase chain reaction with confronting two-pair primers that detects the rs12979860 polymorphism. The assay is internally controlled and does not require the use of restriction endonucleases or special equipment. Moreover, the assay decreases costs, being about 40% cheaper than direct sequencing methods.
authors.affiliation: University of São Paulo School of Medicine, Department of Gastroenterology, São Paulo, SP, Brazil.
authors.forename: Camila da Silva
authors.initials: Cda S
authors.lastname: Ferreira
authors.forename: Rodrigo Martins
authors.initials: RM
authors.lastname: Abreu
authors.forename: Marlone Cunha
authors.initials: MC
authors.lastname: da Silva
authors.forename: Aline Siqueira
authors.initials: AS
authors.lastname: Ferreira
authors.forename: Paulo Dominguez
authors.initials: PD
authors.lastname: Nasser
authors.forename: Flair José
authors.initials: FJ
authors.lastname: Carrilho
authors.forename: Suzane Kioko
authors.initials: SK
authors.lastname: Ono
mesh_terms: D000328:Adult; D017931:DNA Primers / Q000235:genetics; D005260:Female; D055106:Genome-Wide Association Study; D019698:Hepatitis C, Chronic / Q000191:economics / Q000235:genetics*; D006801:Humans; D007372:Interferons; D007378:Interleukins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D000095022:Interferon Lambda
chemical_list: D017931:DNA Primers; D007372:Interferons; D007378:Interleukins; C000624353:interferon-lambda, human; D000095022:Interferon Lambda