title: Biomolecule delivery to engineer the cellular microenvironment for regenerative medicine.
abstract: To realize the potential of regenerative medicine, controlling the delivery of biomolecules in the cellular microenvironment is important as these factors control cell fate. Controlled delivery for tissue engineering and regenerative medicine often requires bioengineered materials and cells capable of spatiotemporal modulation of biomolecule release and presentation. This review discusses biomolecule delivery from the outside of the cell inwards through the delivery of soluble and insoluble biomolecules as well as from the inside of the cell outwards through gene transfer. Ex vivo and in vivo therapeutic strategies are discussed, as well as combination delivery of biomolecules, scaffolds, and cells. Various applications in regenerative medicine are highlighted including bone tissue engineering and wound healing.
authors.affiliation: Department of Biomedical Engineering, The Institute for Nanobiotechnology, Johns Hopkins University School of Medicine, 400 North Broadway, Baltimore, MD, 21231, USA.
authors.forename: Corey J
authors.initials: CJ
authors.lastname: Bishop
authors.forename: Jayoung
authors.initials: J
authors.lastname: Kim
authors.forename: Jordan J
authors.initials: JJ
authors.lastname: Green
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D060833:Cellular Microenvironment; D016503:Drug Delivery Systems / Q000379:methods*; D018014:Gene Transfer Techniques; D006801:Humans; D044968:Regenerative Medicine / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials