title: Staphylococcus haemolyticus disseminated among neonates with bacteremia in a neonatal intensive care unit in Rio de Janeiro, Brazil.
abstract: Oxacillin-resistant Staphylococcus haemolyticus (ORSH) was found as the most prevalent (77.5%) species of coagulase-negative staphylococci associated with bacteremia in neonates making use of intravenous catheters in an intensive care unit of a Brazilian teaching hospital. Thirty-one blood isolates were confirmed as S. haemolyticus by sequencing of the 16S and clustered in 6 pulsed-field gel electrophoresis types (with 58% of the strains belonging to 2 predominant types B and D). S. haemolyticus was mostly oxacillin-resistant (90.3%) displaying multiresistance profiles (70.4%). However, the mecA gene was undetected in 22.6% strains. ORSH exhibited slime production on Congo-Red agar (67.7%), adherence to polystyrene (96.7%), and glass (87%) surfaces. Interestingly, ica-operon was detected in 58% strains, mostly belonging to the B, D, and F genotypes, which is a significantly higher percentage when compared to other studies conducted at different parts of the globe. Data indicated that ica operon and biofilm-forming ORSH are endemic in Brazilian nosocomial environment.
authors.affiliation: Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro.
authors.forename: Paula Marcele Afonso
authors.initials: PM
authors.lastname: Pereira
authors.affiliation: Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro.
authors.forename: Vanessa Batista
authors.initials: VB
authors.lastname: Binatti
authors.affiliation: Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro.
authors.forename: Bruna Pinto Ribeiro
authors.initials: BP
authors.lastname: Sued
authors.affiliation: Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro; Fundação Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Saúde - INCQS -Departamento de Microbiologia, Rio de Janeiro, RJ, Brazil.
authors.forename: Juliana Nunes
authors.initials: JN
authors.lastname: Ramos
authors.affiliation: Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro; Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro.
authors.forename: Renata Stavracakis
authors.initials: RS
authors.lastname: Peixoto
authors.affiliation: Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro.
authors.forename: Cláudio
authors.initials: C
authors.lastname: Simões
authors.affiliation: Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro.
authors.forename: Eduardo Almeida
authors.initials: EA
authors.lastname: de Castro
authors.affiliation: Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro.
authors.forename: José Luís Muniz Bandeira
authors.initials: JL
authors.lastname: Duarte
authors.affiliation: Fundação Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Saúde - INCQS -Departamento de Microbiologia, Rio de Janeiro, RJ, Brazil.
authors.forename: Verônica Viana
authors.initials: VV
authors.lastname: Vieira
authors.affiliation: Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro.
authors.forename: Raphael
authors.initials: R
authors.lastname: Hirata
authors.affiliation: Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro.
authors.forename: Kátia Regina Netto
authors.initials: KR
authors.lastname: Santos
authors.affiliation: Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro. Electronic address: guaraldi@uerj.br.
authors.forename: Ana Luíza
authors.initials: AL
authors.lastname: Mattos-Guaraldi
authors.affiliation: Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro.
authors.forename: José Augusto Adler
authors.initials: JA
authors.lastname: Pereira
mesh_terms: D016470:Bacteremia / Q000453:epidemiology* / Q000382:microbiology; D001422:Bacterial Adhesion; D001938:Brazil / Q000453:epidemiology; D016000:Cluster Analysis; D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006784:Hospitals, Teaching; D006801:Humans; D007231:Infant, Newborn; D007363:Intensive Care Units, Neonatal; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology; D041162:Staphylococcus haemolyticus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D004269:DNA, Bacterial; D011135:Polysaccharides, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Bacteremia; Biofilm; Multiresistance; Neonates; PFGE; Staphylococcus haemolyticus