title: Superior in vivo compatibility of hydrophilic polymer coated prosthetic vascular grafts.
abstract: OBJECTIVE
  Protein adsorption, cell adhesion and graft patency was compared in hydrophilic versus hydrophobic polymer-coated prosthetic vascular grafts. We hypothesize that in vivo compatibility of hydrophilic polymer-coated prosthetic vascular grafts is superior to in vivo compatibility of hydrophobic grafts.
  
  
  METHODS
  A pairwise side-to-side common carotid artery interposition graft was placed eight female landrace goats (mean weight 55 kg). Protein adsorption was assessed using Western Blot in two hydrophilic and two hydrophobic grafts harvested after three days. Graft patency was monitored for 28 days in six goats with continuous wave Doppler ultrasonography. Adherence of endothelial cells, leukocytes and platelets was determined with ELISA and compared between the two graft types after 28 days.
  
  
  RESULTS
  After three days, more ApoA-I, albumin and VEGF and less fibrin adsorbed to hydrophilic grafts. After 28 days, compared to hydrophobic grafts, higher numbers of endothelial cells were present on hydrophilic grafts (P=0.016), and less thrombocytes and leukocytes (P=0.012 and 0.024, respectively). Two out of eight hydrophobic grafts lost patency, while none of the hydrophilic grafts failed (P=0.157).
  
  
  CONCLUSIONS
  Hydrophilic polymer-coated vascular grafts have superior in vivo compatibility when compared to hydrophobic grafts as characterized by reduced platelet and leukocyte adherence as well as higher endothelialization.
authors.affiliation: Department of Vascular Medicine, Academic Medical Center/University of Amsterdam, Amsterdam - The Netherlands.
authors.forename: Aart C
authors.initials: AC
authors.lastname: Strang
authors.forename: Menno L W
authors.initials: ML
authors.lastname: Knetsch
authors.forename: Mirza M
authors.initials: MM
authors.lastname: Idu
authors.forename: Radjesh J
authors.initials: RJ
authors.lastname: Bisoendial
authors.forename: Gertjan
authors.initials: G
authors.lastname: Kramer
authors.forename: Dave
authors.initials: D
authors.lastname: Speijer
authors.forename: Leo H
authors.initials: LH
authors.lastname: Koole
authors.forename: Erik S G
authors.initials: ES
authors.lastname: Stroes
authors.forename: Joris I
authors.initials: JI
authors.lastname: Rotmans
mesh_terms: D000327:Adsorption; D000818:Animals; D001807:Blood Vessel Prosthesis; D019917:Blood Vessel Prosthesis Implantation / Q000009:adverse effects / Q000295:instrumentation*; D015153:Blotting, Western; D017536:Carotid Artery, Common / Q000000981:diagnostic imaging / Q000503:physiopathology / Q000601:surgery*; D002448:Cell Adhesion; D020099:Coated Materials, Biocompatible; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006041:Goats; D006083:Graft Occlusion, Vascular / Q000209:etiology / Q000503:physiopathology; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D023421:Models, Animal; D010973:Platelet Adhesiveness; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D011474:Prosthesis Design; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties; D013997:Time Factors; D018608:Ultrasonography, Doppler; D014654:Vascular Patency
chemical_list: D020099:Coated Materials, Biocompatible; D011108:Polymers; D011506:Proteins