title: Double-bed-type extraction needle packed with activated-carbon-based sorbents for very volatile organic compounds.
abstract: A novel needle-type sample preparation device was developed for the determination of very volatile organic compounds (VVOCs) in gaseous samples by gas chromatography-mass spectrometry (GC-MS). Two types of activated-carbon-based sorbents, Carbopack X and a carbon molecular sieve (CMS), were investigated as the extraction medium. A double-bed-type extraction needle showed successful extraction and desorption performance for all investigated VVOCs, including acetaldehyde, isoprene, pentane, acetone, and ethanol. Sensitive and reliable determination of VVOCs was achieved by systematically optimizing several desorption conditions. In addition, the effects of sample humidity on the extraction and desorption of analytes were investigated with the needle-type extraction devices. Only the CMS packed extraction needle was adversely affected by sample humidity during the desorption process; on the other hand the double-bed-type extraction needle was unaffected by sample humidity. Finally, the developed double-bed-type extraction needle was successfully applied to the analysis of breath VVOCs of healthy subjects.
authors.affiliation: Department of Applied Chemistry, University of Yamanashi, 4-3-11 Takeda, Kofu 400-8511, Japan. Electronic address: iueta@yamanashi.ac.jp.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Ueta
authors.forename: Emi Liana
authors.initials: EL
authors.lastname: Samsudin
authors.forename: Ayako
authors.initials: A
authors.lastname: Mizuguchi
authors.forename: Hayato
authors.initials: H
authors.lastname: Takeuchi
authors.forename: Takumi
authors.initials: T
authors.lastname: Shinki
authors.forename: Susumu
authors.initials: S
authors.lastname: Kawakubo
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Saito
mesh_terms: D000079:Acetaldehyde / Q000737:chemistry; D000327:Adsorption; D001944:Breath Tests / Q000295:instrumentation / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D002626:Chemistry, Pharmaceutical; D008401:Gas Chromatography-Mass Spectrometry; D005740:Gases; D064368:Healthy Volunteers; D006801:Humans; D057230:Limit of Detection; D009339:Needles; D013696:Temperature; D055549:Volatile Organic Compounds / Q000032:analysis*; D014835:Volatilization
chemical_list: D005740:Gases; D055549:Volatile Organic Compounds; D002244:Carbon; D000079:Acetaldehyde
keywords: Breath analysis; Gas chromatography; Needle extraction; Sample preparation; Very volatile organic compounds