title: Association of a DNA marker with Hessian fly resistance gene H9 in wheat.
abstract: The Hessian fly [Mayetiola destructor (Say)] is a major pest of wheat (Triticum aestivum L.) and genetic resistance has been used effectively over the past 30 years to protect wheat against serious damage by the fly. To-date, 25 Hessian fly resistance genes, designated H1 to H25, have been identified in wheat. With near-isogenic wheat lines differing for the presence of an individual Hessian fly resistance gene, in conjunction with random amplified polymorphic DNA (RAPD) analysis and denaturing gradient-gel electrophoresis (DGGE), we have identified a DNA marker associated with the H9 resistance gene. The H9 gene confers resistance against biotype L of the Hessian fly, the most virulent biotype. The RAPD marker cosegregates with resistance in a segregating F2 population, remains associated with H9 resistance in a number of different T. aestivum and T. durum L. genetic backgrounds, and is readily detected by either DGGE or DNA gel-blot hybridization.
authors.affiliation: Department of Agronomy, Purdue University, 47907, West Lafayette, IN, USA.
authors.forename: I
authors.initials: I
authors.lastname: Dweikat
authors.forename: H
authors.initials: H
authors.lastname: Ohm
authors.forename: S
authors.initials: S
authors.lastname: Mackenzie
authors.forename: F
authors.initials: F
authors.lastname: Patterson
authors.forename: S
authors.initials: S
authors.lastname: Cambron
authors.forename: R
authors.initials: R
authors.lastname: Ratcliffe