title: Inhibition of Nitric Oxide Synthase by L-NAME Promotes Cisplatin-Induced Nephrotoxicity in Male Rats.
abstract: Objective. Nitric oxide (NO) has numerous important functions in the kidney. The role of NO in cisplatin (CP)-induced nephrotoxicity is not completely understood. This study was designed to determine the role of NO synthase inhibitor (L-NAME) on the severity of CP-induced nephrotoxicity in rats. Methods. Sixty four male (M) and female (F) Wistar rats were randomly divided into eight groups. The sham groups (group 1, male, n = 6 and group 2, female, n = 6) received saline. Groups 3 (male, n = 8) and 4 (female, n = 8) were treated with L-NAME (4 mg/kg, i.p.), and groups 5 (male, n = 8) and 6 (female, n = 8) received CP (3 mg/kg) for 7 days. Groups 7 (male, n = 8) and 8 (female, n = 8) were treated with L-NAME and CP for 7 days. Results. The CP-alone treated rats showed weight loss and increase in serum levels of blood urea nitrogen (BUN) and creatinine (Cr). Coadministration of L-NAME and CP did not improve weight loss, and it increased the levels of BUN and Cr in male but not in female rats (P < 0.05). CP alone increased kidney damage significantly (P < 0.05 ), however, the damage induced by combination of CP and L-NAME was gender-related. Conclusion. NOS inhibition by L-NAME increased CP-induced nephrotoxicity, which was gender-related.
authors.affiliation: Water & Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan 81745, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Moslemi
authors.forename: Mehdi
authors.initials: M
authors.lastname: Nematbakhsh
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Eshraghi-Jazi
authors.forename: Ardeshir
authors.initials: A
authors.lastname: Talebi
authors.forename: Hamid
authors.initials: H
authors.lastname: Nasri
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Ashrafi
authors.forename: Maryam
authors.initials: M
authors.lastname: Moeini
authors.forename: Azam
authors.initials: A
authors.lastname: Mansouri
authors.forename: Zahra
authors.initials: Z
authors.lastname: Pezeshki