title: A method to measure cellular adhesion utilizing a polymer micro-cantilever.
abstract: In the present study we engineered a micro-machined polyimide cantilever with an embedded sensing element to investigate cellular adhesion, in terms of its relative ability to stick to a cross-linker, 3,3'-dithiobis[sulfosuccinimidylpropionate], coated on the cantilever surface. To achieve this objective, we investigated adhesive properties of three human prostate cancer cell lines, namely, a bone metastasis derived human prostate cancer cell line (PC3), a brain metastasis derived human prostate cancer cell line (DU145), and a subclone of PC3 (PC3-EMT14). We found that PC3-EMT14, which displays a mesenchymal phenotype, has the least adhesion compared to PC3 and DU145, which exhibit an epithelial phenotype.
authors.affiliation: PicoCal, Inc., 333 Parkland Plaza, Ann Arbor, Michigan 48103, USA ; Electronic Instrumentation Laboratory, TU Delft, Mekelweg 4, 2628CD Delft, The Netherlands.
authors.forename: Angelo
authors.initials: A
authors.lastname: Gaitas
authors.forename: Ricky
authors.initials: R
authors.lastname: Malhotra
authors.forename: Kenneth
authors.initials: K
authors.lastname: Pienta