title: Actin cytoskeleton reorganization correlates with polarization of secretory vesicle and cell morphology in the degranulation of mast cell subtypes in human colon tissues.
abstract: Mast cells play a central role in the intestinal immune response. To investigate the relationship between degranulation, cell polarization and the reorganization of actin cytoskeleton of mast cells, we used fluorescence or gold labeling methods to identify different mast cell subtypes in human colon. The reorganization of filamentous actin was visualized and then the polarization of secretory vesicles, as well as cell surfaces, was analyzed by fluorescence microscopy and electron microscopy. Our results first showed a diversity of filamentous actin assembly or disassembly within the contacting cell membrane of different mast cell subtypes. The polarization and degranulation of secretory vesicles was not only accompanied with the assembly and disassembly of filamentous actin at the cell periphery, but also with changes of cell surface polarization. Our study provides an insight into the local membranous structures and suggested correlations of cytoskeleton arrangement with the polarization of secretory vesicles and cell surface configuration during mast cell degranulation.
authors.affiliation: Laboratory of Analytical Cytology, Shantou University Medical College, Shantou 515041, People's Republic of China. Electronic address: lin.juelong@qq.com.
authors.forename: Jue-Long
authors.initials: JL
authors.lastname: Lin
authors.affiliation: Laboratory of Analytical Cytology, Shantou University Medical College, Shantou 515041, People's Republic of China.
authors.forename: Chun-Gui
authors.initials: CG
authors.lastname: Chen
authors.affiliation: First Affiliated Hospital, Shantou University Medical College, Shantou 515041, People's Republic of China.
authors.forename: Zhi-Zhong
authors.initials: ZZ
authors.lastname: Shen
authors.affiliation: Laboratory of Analytical Cytology, Shantou University Medical College, Shantou 515041, People's Republic of China.
authors.forename: Zhong-Xian
authors.initials: ZX
authors.lastname: Piao
authors.affiliation: Laboratory of Analytical Cytology, Shantou University Medical College, Shantou 515041, People's Republic of China.
authors.forename: Wei-Qiu
authors.initials: WQ
authors.lastname: Li
authors.affiliation: Laboratory of Analytical Cytology, Shantou University Medical College, Shantou 515041, People's Republic of China.
authors.forename: Liu
authors.initials: L
authors.lastname: Liu
authors.affiliation: Institute of Oncologic Pathology, Shantou University Medical College, Shantou 515041, People's Republic of China.
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Xu
authors.affiliation: Department of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou 515041, People's Republic of China.
authors.forename: En-Min
authors.initials: EM
authors.lastname: Li
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D015550:Cell Degranulation; D016764:Cell Polarity; D003106:Colon / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D005434:Flow Cytometry; D006801:Humans; D008407:Mast Cells / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D018613:Microscopy, Confocal; D022142:Secretory Vesicles / Q000378:metabolism*
keywords: Filamentous actin reorganization; Mast cell subtypes; Secretory vesicle