title: An experimental approach to the generation of human embryonic stem cells equivalents.
abstract: Recently, particular attention has been paid to the human embryonic stem cells (hESC) in the context of their potential application in regenerative medicine; however, ethical concerns prevent their clinical application. Induction of pluripotency in somatic cells seems to be a good alternative for hESC recruitment regarding its potential use in tissue regeneration, disease modeling, and drug screening. Since Yamanaka's team in 2006 restored pluripotent state of somatic cells for the first time, a significant progress has been made in the area of induced pluripotent stem cells (iPSC) generation. Here, we review the current state of knowledge in the issue of techniques applied to establish iPSC. Somatic cell nuclear transfer, cell fusion, cell extracts reprogramming, and techniques of direct reprogramming are described. Retroviral and lentiviral transduction are depicted as ways of cell reprogramming with the use of integrating vectors. Contrary to them, adenoviruses, plasmids, single multiprotein expression vectors, and PiggyBac transposition systems are examples of non-integrative vectors used in iPSC generation protocols. Furthermore, reprogramming with the delivery of specific proteins, miRNA or small chemical compounds are presented. Finally, the changes occurring during the reprogramming process are described. It is concluded that subject to some limitations iPSC could become equivalents for hESC in regenerative medicine.
authors.affiliation: Students Scientific Society, Medical University of Silesia, Katowice, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Skowron
authors.forename: Marcin
authors.initials: M
authors.lastname: Tomsia
authors.forename: Piotr
authors.initials: P
authors.lastname: Czekaj
mesh_terms: D060846:Cell Engineering / Q000379:methods*; D002459:Cell Fusion; D065150:Cellular Reprogramming; D053595:Embryonic Stem Cells / Q000166:cytology*; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D053652:Nuclear Transfer Techniques; D044968:Regenerative Medicine; D012190:Retroviridae / Q000235:genetics*; D014161:Transduction, Genetic / Q000379:methods*