title: Mimicking bone microenvironment for directing adipose tissue-derived mesenchymal stem cells into osteogenic differentiation.
abstract: Adipose tissue-derived mesenchymal stem cells (ASCs) have become an increasingly interested cell source for the scientists in the fields of stem cell biology and regenerative medicine. ASCs have already been used in a number of clinical trials, and some successful outcomes have been reported in bone tissue regeneration. Here we describe the protocols which mimic the factors in bone healing microenvironment, including inflammation burst, osteoblasts, and bone biomimetic scaffolds to direct ASCs into osteogenic differentiation.
authors.affiliation: Biomaterials and Tissue Engineering Research Unit, School of AMME, The University of Sydney, Sydney, Australia.
authors.forename: ZuFu
authors.initials: Z
authors.lastname: Lu
authors.forename: Seyed-Iman
authors.initials: SI
authors.lastname: Roohani-Esfahani
authors.forename: Hala
authors.initials: H
authors.lastname: Zreiqat
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D040761:Biomimetic Materials / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D060833:Cellular Microenvironment / Q000187:drug effects*; D018920:Coculture Techniques; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D053758:Nanoparticles / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D014409:Tumor Necrosis Factor-alpha; D017886:Durapatite