title: Absorption, metabolism and effect of compatibility on absorption of qishenyiqi dropping pill.
abstract: Qishenyiqi dropping pill (QSYQ), is a traditional Chinese medicine (TCM) prescription for treating heart diseases in China. Knowledge concerning the systemic identification of active compounds and metabolic components of QSYQ is generally lacking. Therefore, it is essential to develop a valid method for the analysis of active compounds of the combined prescription and determination of interactions among the herbs. The absorbable compounds and metabolites of QSYQ were profiled using computational chemistry prediction, an improved everted gut sac in vitro experiment, the Caco-2 cell monolayer in vitro test, a rat in vivo experiment and ultra-performance liquid chromatography/diode array detection/quadrupole-time of flight mass spectrum (UPLC/DAD/Q-TOF MS). In total, 42 prototype compounds were recognized as absorbable compounds, and eight metabolites were identified by UPLC/DAD/Q-TOF MS. The absorption rates of phenolic acids and saponins were significantly improved and the absorption of isoflavone was inhibited after compatibility. The volatile oil component had an improved effect on the absorption of other compounds, while its own absorption was inhibited. In conclusion, the present study established a rapid and effective strategy for demonstrating the absorption and metabolism of QSYQ and revealing the compatible relationship among herbs. This investigation can provide a reference for the compatibility of prescriptions and the modernization of TCM.
authors.affiliation: College of Pharmacy, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, 300071, People's Republic of China.
authors.forename: Yan-Qi
authors.initials: YQ
authors.lastname: Han
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Qing-Xin
authors.initials: QX
authors.lastname: Cui
authors.forename: Li-Qiang
authors.initials: LQ
authors.lastname: Wang
authors.forename: Bin-Feng
authors.initials: BF
authors.lastname: Cheng
authors.forename: Hong-Zhi
authors.initials: HZ
authors.lastname: Zhao
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Gang
authors.initials: G
authors.lastname: Bai
authors.forename: Guo-An
authors.initials: GA
authors.lastname: Luo
mesh_terms: D000042:Absorption; D000818:Animals; D018938:Caco-2 Cells; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003198:Computer Simulation; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics*; D041743:Herb-Drug Interactions; D006801:Humans; D007421:Intestine, Small / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; C528580:qishen yiqi
keywords: QSYQ; UPLC/Q-TOF MS; absorption; compatibility; metabolism