title: Kordia jejudonensis sp. nov., isolated from the junction between the ocean and a freshwater spring, and emended description of the genus Kordia.
abstract: A Gram-staining-negative, aerobic, non-spore-forming, non-flagellated, non-gliding and rod-shaped bacterial strain, SSK3-3(T), was isolated from the zone where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain SSK3-3(T) grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain SSK3-3(T) clustered with the type strains of species of the genus Kordia, with which it exhibited 96.5-97.2% 16S rRNA gene sequence similarity. Sequence similarities to the type strains of other recognized species were less than 92.2%. Strain SSK3-3(T) contained MK-6 as the predominant menaquinone and iso-C(15 : 0), iso-C(17 : 0) 3-OH, iso-C(15 : 0) 3-OH and summed feature 3 (C(16 : 1)ω7c and/or C(16 : 1)ω6c) as the major fatty acids. The major polar lipids of strain SSK3-3(T) were phosphatidylethanolamine, one unidentified lipid and one unidentified glycolipid. The DNA G+C content of strain SSK3-3(T) was 34.9 mol% and the mean DNA-DNA relatedness with Kordia periserrulae KACC 14311(T) was 12%. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain SSK3-3(T) is separate from other species of the genus Kordia. On the basis of the data presented, strain SSK3-3(T) is considered to represent a novel species of the genus Kordia, for which the name Kordia jejudonensis sp. nov. is proposed. The type strain is SSK3-3(T) ( = KCTC 32426(T) = CECT 8368(T)). An emended description of the genus Kordia is also provided.
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005618:Fresh Water / Q000382:microbiology*; D006017:Glycolipids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6