title: Molecularly imprinted photonic polymer based on β-cyclodextrin for amino acid sensing.
abstract: A novel molecularly imprinted photonic polymer (MIPP) using maleic anhydride modified β-cyclodextrin (β-CD) and acrylic acid as functional monomers has been presented for amino acid sensing. Reactive β-CD monomer carrying vinyl carboxylic acid functional groups was first synthesized. MIPP was fabricated by filling precursor solution into the interstitial spaces of polystyrene photonic crystal templates, followed by a thermal polymerization at 55 °C. Characterization showed that the MIPP possessed an opal photonic crystal structure. This β-CD-based MIPP could undergo a swelling change from 590 nm to 704 nm and still retain the molecular imprinting recognition ability during the sensing of L-phenylalanine (L-Phe). A function relationship was found between the diffraction wavelength shift and the logarithm of L-Phe concentration in the range of 10(-8)M to 10(-4)M at pH 6. A wavelength shift of 114 nm for L-Phe was observed within 30s, whereas there were no obvious shifts for d-Phe, L-tyrosine and L-tryptophan, indicating that the β-CD-based MIPP had high specificity and rapid response to L-Phe. The developed MIPP sensor has been applied to detect L-Phe in compound amino acid injection sample.
authors.affiliation: Department of Chemistry, School of Science, Tianjin University, Tianjin 300072, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Liu
authors.forename: Hong-Xun
authors.initials: HX
authors.lastname: Fang
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Yu
mesh_terms: D000179:Acrylates / Q000737:chemistry; D003460:Crystallization; D006863:Hydrogen-Ion Concentration; D008299:Maleic Anhydrides / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods*; D010649:Phenylalanine / Q000032:analysis*; D017785:Photons; D058105:Polymerization; D011137:Polystyrenes / Q000737:chemistry; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D013696:Temperature; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000179:Acrylates; D008299:Maleic Anhydrides; D011137:Polystyrenes; D047392:beta-Cyclodextrins; D010649:Phenylalanine; C036658:acrylic acid; C031215:betadex
keywords: Molecular imprinting; Molecularly imprinted photonic polymer; Phenylalanine; Photonic crystal; β-cyclodextrin