title: An efficient strategy for the extraction and purification of lignans from Schisandra chinensis by a combination of supercritical fluid extraction and high-speed counter-current chromatography.
abstract: An efficient strategy for extracting and separating five lignans from Schisandra chinensis (Turcz.) Baill has been developed using supercritical fluid extraction (SFE) and high-speed counter-current chromatography (HSCCC) in the present study. First, the extraction was performed by a preparative SFE system under 15 MPa of pressure at 36°C for 4 h. Then, the SFE extract was successfully separated and purified by HSCCC with a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (6:4:5:5, 6:4:6:4, 6:4:8:2, v/v) in a stepwise elution mode. The fractions were analyzed by HPLC, and the chemical structures of the products were identified by ESI-MS and 1H NMR spectroscopy. As a result, a total of 12.5 mg of schisandrin at 98.0% purity, 7.1 mg of gomisin A at 98.1% purity, 1.8 mg of schisantherin B at 93.3% purity, 4.4 mg of deoxyschisandrin at 92.9% purity, and 6.8 mg of γ-schisandrin at 89.1% purity were obtained from 300 mg crude extract in a one-step purification.
authors.forename: Chu
authors.initials: C
authors.lastname: Chu
authors.forename: Shidi
authors.initials: S
authors.lastname: Zhang
authors.forename: Shengqiang
authors.initials: S
authors.lastname: Tong
authors.forename: Xingnuo
authors.initials: X
authors.lastname: Li
authors.forename: Jizhong
authors.initials: J
authors.lastname: Yan
mesh_terms: D025924:Chromatography, Supercritical Fluid; D003377:Countercurrent Distribution; D017705:Lignans / Q000302:isolation & purification*; D008968:Molecular Conformation; D032141:Schisandra / Q000737:chemistry*
chemical_list: D017705:Lignans