title: Live cell chemical profiling of temporal redox dynamics in a photoautotrophic cyanobacterium.
abstract: Protein reduction-oxidation (redox) modification is an important mechanism that allows microorganisms to sense environmental changes and initiate cellular responses. We have developed a quantitative chemical probe approach for live cell labeling and imaging of proteins that are sensitive to redox modifications. We utilize this in vivo strategy to identify 176 proteins undergoing ∼5-10-fold dynamic redox change in response to nutrient limitation and subsequent replenishment in the photoautotrophic cyanobacterium Synechococcus sp. PCC 7002. We detect redox changes in as little as 30 s after nutrient perturbation and oscillations in reduction and oxidation for 60 min following the perturbation. Many of the proteins undergoing dynamic redox transformations participate in the major components for the production (photosystems and electron transport chains) or consumption (Calvin-Benson cycle and protein synthesis) of reductant and/or energy in photosynthetic organisms. Thus, our in vivo approach reveals new redox-susceptible proteins and validates those previously identified in vitro.
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352, United States.
authors.forename: Natalie C
authors.initials: NC
authors.lastname: Sadler
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Melnicki
authors.forename: Margrethe H
authors.initials: MH
authors.lastname: Serres
authors.forename: Eric D
authors.initials: ED
authors.lastname: Merkley
authors.forename: William B
authors.initials: WB
authors.lastname: Chrisler
authors.forename: Eric A
authors.initials: EA
authors.lastname: Hill
authors.forename: Margaret F
authors.initials: MF
authors.lastname: Romine
authors.forename: Sangtae
authors.initials: S
authors.lastname: Kim
authors.forename: Erika M
authors.initials: EM
authors.lastname: Zink
authors.forename: Suchitra
authors.initials: S
authors.lastname: Datta
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Beliaev
authors.forename: Allan
authors.initials: A
authors.lastname: Konopka
authors.forename: Aaron T
authors.initials: AT
authors.lastname: Wright
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D015335:Molecular Probes / Q000378:metabolism*; D061848:Optical Imaging; D010084:Oxidation-Reduction; D014176:Protein Biosynthesis; D046940:Synechococcus / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D015335:Molecular Probes