title: Measurement of microbial DNA polymerase activity enables detection and growth monitoring of microbes from clinical blood cultures.
abstract: Surveillance of bloodstream infections (BSI) is a high priority within the hospital setting. Broth-based blood cultures are the current gold standard for detecting BSI, however they can require lengthy incubation periods prior to detection of positive samples. We set out to demonstrate the feasibility of using enzymatic template generation and amplification (ETGA)-mediated measurement of DNA polymerase activity to detect microbes from clinical blood cultures. In addition to routine-collected hospital blood cultures, one parallel aerobic blood culture was collected and immediately refrigerated until being transported for ETGA analysis. After refrigeration holding and transport, parallel-collected cultures were placed into a BACTEC incubator and ETGA time-course analysis was performed. Of the 308 clinical blood cultures received, 22 were BACTEC positive, and thus were initially selected for ETGA time course analysis. The ETGA assay detected microbial growth in all 22 parallel-positive blood cultures in less time than a BACTEC incubator and also yielded genomic DNA for qPCR-based organism identification. In summary, feasibility of detecting microbes from clinical blood culture samples using the ETGA blood culture assay was demonstrated. Additional studies are being considered towards development of clinically beneficial versions of this methodology.
authors.affiliation: Research and Development, ZEUS Scientific, Raritan, New Jersey, United States of America.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Zweitzig
authors.forename: Nichol M
authors.initials: NM
authors.lastname: Riccardello
authors.forename: John
authors.initials: J
authors.lastname: Morrison
authors.forename: Jason
authors.initials: J
authors.lastname: Rubino
authors.forename: Jennifer
authors.initials: J
authors.lastname: Axelband
authors.forename: Rebecca
authors.initials: R
authors.lastname: Jeanmonod
authors.forename: Bruce I
authors.initials: BI
authors.lastname: Sodowich
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Kopnitsky
authors.forename: S Mark
authors.initials: SM
authors.lastname: O'Hara
mesh_terms: D000328:Adult; D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology*; D001419:Bacteria / Q000201:enzymology* / Q000254:growth & development* / Q000302:isolation & purification; D004269:DNA, Bacterial; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D004259:DNA-Directed DNA Polymerase