title: Production and characteristics of a bioflocculant by Klebsiella pneumoniae YZ-6 isolated from human saliva.
abstract: The production and characterization of a bioflocculant, MBF-6, by Klebsiella pneumoniae was investigated. Optimum culture conditions for bioflocculant production were an initial medium pH of 7, an incubation temperature of 30 °C, and an inoculum size of 1% (v/v) of cell density 1.0 × 10(8) cfu/mL. The carbon, nitrogen, and cation sources for optimum bioflocculant production were glucose, peptone, and ZnCl₂. The bioflocculant mainly consisted of protein (3.4%) and sugar (95.1%). Fourier transform infrared (FTIR) spectrum revealed the presence of carboxyl and hydroxyl groups while the thermogravimetric analysis (TGA) showed a degradation temperature (T(d)) of 81.4 °C. MBF-6 had a good flocculating rate in kaolin suspension without cation addition and was stable over a wide range of pH and temperature. Investigation on the flocculation efficacy of the characterized MBF-6 for wastewater treatment of dairy, woolen, brewery, and sugar industries suggested it to be effective.
authors.affiliation: School of Environmental Engineering, Wuhan Textile University, FangZhi Road, Wuhan, 430073, China.
authors.forename: Zhengshan
authors.initials: Z
authors.lastname: Luo
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Changhong
authors.initials: C
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.forename: Ye
authors.initials: Y
authors.lastname: Han
authors.forename: Jiangang
authors.initials: J
authors.lastname: Zhou
mesh_terms: D002244:Carbon / Q000378:metabolism; D005428:Flocculation Tests; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007711:Klebsiella pneumoniae / Q000254:growth & development / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D012463:Saliva / Q000378:metabolism / Q000382:microbiology*; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry
chemical_list: D002244:Carbon; D009584:Nitrogen