title: Enhancement of ansamitocin P-3 production in Actinosynnema pretiosum by a synergistic effect of glycerol and glucose.
abstract: Ansamitocin P-3 (AP-3), a secondary metabolite produced by Actinosynnema pretiosum, is well known for its extraordinary antitumor properties and is broadly utilized in clinical research. Through this work, we found, for the first time, that the combination of glucose and glycerol as a mixed carbon source is an appropriate approach for enhancing the production of AP-3 by A. pretiosum. The amount yielded was about threefold that obtained with glucose as the sole carbon source. In order to better understand the mechanisms that channel glycerol metabolism towards AP-3 production, the activities of some key enzymes such as glucose-6-phosphate dehydrogenase, glucose-6-phosphate isomerase, phosphoglucomutase (PGM), and fructose 1,6-bisphosphatase were assessed. The results showed that glycerol affects the production of AP-3 by increasing PGM activity. Furthermore, qRT-PCR analysis revealed that transcriptional levels of structural genes asm14 and asm24, and primary genes amir5189 and amir6327 were up-regulated in medium containing glycerol.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Fan
authors.forename: Komi
authors.initials: K
authors.lastname: Nambou
authors.forename: Liujing
authors.initials: L
authors.lastname: Wei
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Liu
authors.forename: Tadayuki
authors.initials: T
authors.lastname: Imanaka
authors.forename: Qiang
authors.initials: Q
authors.lastname: Hua
mesh_terms: D000192:Actinomycetales / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D008453:Maytansine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry; D014158:Transcription, Genetic
chemical_list: D008453:Maytansine; C015682:ansamitocins; D002244:Carbon; D005947:Glucose; D005990:Glycerol