title: Cholesterol determination using protein-templated fluorescent gold nanocluster probes.
abstract: We describe the development of a fluorescent biosensor platform for soluble cholesterol based on bovine serum albumin-stabilized gold nanocluster probes co-dissolved with cholesterol oxidase (ChOx) in a surfactant emulsion system. Selective enzymatic oxidation of cholesterol to cholest-4-en-3-one by ChOx produces stoichiometric amounts of H2O2 by-product, generating a quenching response signaling the presence of cholesterol at clinically relevant levels (LOD ∼12 μM).
authors.affiliation: Department of Chemistry, University of Missouri-Columbia, Columbia, MO 65211, USA. bakergar@missouri.edu.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Gary A
authors.initials: GA
authors.lastname: Baker
mesh_terms: D002784:Cholesterol / Q000032:analysis*; D005453:Fluorescence; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles; D010084:Oxidation-Reduction; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins; D006046:Gold; D002784:Cholesterol