title: LARP1 predict the prognosis for early-stage and AFP-normal hepatocellular carcinoma.
abstract: BACKGROUND
  The La-related protein 1 (LARP1) has been found to be a RNA binding protein and was related to spermatogenesis, embryogenesis and cell-cycle progression. The aim of this study was to investigate the prognostic value of LARP1 in hepatocellular carcinoma (HCC).
  
  
  METHODS
  LARP1 expression was examined in 15 HCC cell lines and 272 clinical specimens using real-time PCR, immunohistochemistry (IHC) and western blot analysis (WB). LARP1 expression was also studied in 6 paired HCC lesions and the adjacent non-cancerous tissue samples. Statistical analyses were applied to derive association between LARP1 expression scores and clinical characters as well as patient survival.
  
  
  RESULTS
  mRNA and protein levels of LARP1 were higher in HCC cell lines and HCC lesions than in normal liver epithelial cells and the paired adjacent noncancerous tissues. LARP1 expression was correlated to survival time, vital status, tumor size and Child-Pugh score. Overall survival analysis showed HCC patients with high LARP1 expression level had lower survival rate (P<0.01). Importantly, this correlation remained significant in patients with early-stage HCC or with normal serum AFP level.
  
  
  CONCLUSIONS
  LARP1 protein may represent a promising biomarker for predicting the prognosis of HCC, including in early-stage and AFP-normal patients.
authors.affiliation: Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen University, 600# TianHe Road, Guangzhou 510630, Guangdong Province, China. happyxiechan@gmail.com.
authors.forename: Chan
authors.initials: C
authors.lastname: Xie
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.forename: Shibin
authors.initials: S
authors.lastname: Xie
authors.forename: Dongying
authors.initials: D
authors.lastname: Xie
authors.forename: Genlin
authors.initials: G
authors.lastname: Zhang
authors.forename: Peipei
authors.initials: P
authors.lastname: Wang
authors.forename: Liang
authors.initials: L
authors.lastname: Peng
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Gao
mesh_terms: D001324:Autoantigens / Q000502:physiology*; D001483:Base Sequence; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D017931:DNA Primers; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012261:Ribonucleoproteins / Q000502:physiology*; D000509:alpha-Fetoproteins / Q000378:metabolism*; D000097585:SS-B Antigen
chemical_list: D001324:Autoantigens; D017931:DNA Primers; D012261:Ribonucleoproteins; D000509:alpha-Fetoproteins; D000097585:SS-B Antigen