title: FancJ regulates interstrand crosslinker induced centrosome amplification through the activation of polo-like kinase 1.
abstract: DNA damage response (DDR) and the centrosome cycle are two of the most critical processes for maintaining a stable genome in animals. Sporadic evidence suggests a connection between these two processes. Here, we report our findings that six Fanconi Anemia (FA) proteins, including FancI and FancJ, localize to the centrosome. Intriguingly, we found that the localization of FancJ to the mother centrosome is stimulated by a DNA interstrand crosslinker, Mitomycin C (MMC). We further show that, in addition to its role in interstrand crosslinking (ICL) repair, FancJ also regulates the normal centrosome cycle as well as ICL induced centrosome amplification by activating the polo-like kinase 1 (PLK1). We have uncovered a novel function of FancJ in centrosome biogenesis and established centrosome amplification as an integral part of the ICL response.
authors.affiliation: Basic Biomedical Science Division, Sanford School of Medicine, University of South Dakota , Vermillion, South Dakota, 57069 , USA.
authors.forename: Jianqiu
authors.initials: J
authors.lastname: Zou
authors.forename: Fen
authors.initials: F
authors.lastname: Tian
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.forename: Wyatt
authors.initials: W
authors.lastname: Pickner
authors.forename: Molly
authors.initials: M
authors.lastname: Long
authors.forename: Khosrow
authors.initials: K
authors.lastname: Rezvani
authors.forename: Hongmin
authors.initials: H
authors.lastname: Wang
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
keywords: Centrosome; FancJ; PLK1