title: Transplantation of enteric neural stem/progenitor cells into the irradiated young mouse hippocampus.
abstract: Radiotherapy is an effective treatment for brain tumors but often results in cognitive deficits in survivors. Transplantation of embryonic or brain-derived neural stem/progenitor cells (BNSPCs) ameliorated cognitive impairment after irradiation (IR) in animal models. However, such an approach in patients requires a clinically relevant source of cells. We show for the first time the utilization of enteric neural stem/progenitor cells (ENSPCs) from the postnatal intestinal wall as a source of autologous cells for brain repair after injury caused by IR. Cells were isolated from the intestinal wall and propagated in vitro for 1 week. Differentiation assays showed that ENSPCs are multipotent and generated neurons, astrocytes, and myofibroblasts. To investigate whether ENSPCs can be used in vivo, postnatal day 9 mice were subjected to a single moderate irradiation dose (6 or 8 Gy). Twelve days later, mice received an intrahippocampal injection of syngeneic ENSPCs. Four weeks after transplantation, 0.5% and 1% of grafted ENSPCs were detected in the dentate gyrus of sham and irradiated animals, respectively, and only 0.1% was detected after 16 weeks. Grafted ENSPCs remained undifferentiated but failed to restore IR-induced loss of BNSPCs and the subsequent impaired growth of the dentate gyrus. We observed microglia activation, astrogliosis, and loss of granule neurons associated with grafted ENSPC clusters. Transplantation of ENSPCs did not ameliorate IR-induced impaired learning and memory. In summary, while autologous ENSPC grafting to the brain worked technically, even in the absence of immunosuppression, the protocols need to be modified to improve survival and integration.
authors.affiliation: Karolinska Institute, Department of Women's and Children's Health, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Osman
authors.forename: Kai
authors.initials: K
authors.lastname: Zhou
authors.forename: Changlian
authors.initials: C
authors.lastname: Zhu
authors.forename: Klas
authors.initials: K
authors.lastname: Blomgren
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation / Q000528:radiation effects; D017615:Enteric Nervous System / Q000166:cytology*; D005260:Female; D006624:Hippocampus / Q000166:cytology* / Q000528:radiation effects*; D007249:Inflammation / Q000473:pathology; D007259:Infrared Rays; D007858:Learning; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000378:metabolism / Q000528:radiation effects; D058953:Neural Stem Cells / Q000166:cytology* / Q000637:transplantation*; D055495:Neurogenesis / Q000528:radiation effects