title: The effect of a temperature-sensitive poloxamer-alginate-CaCl2 mixture after strabismus surgery in a rabbit model.
abstract: OBJECTIVE
  To determine the efficacy of a temperature-sensitive poloxamer-alginate mixture in reducing adhesions after strabismus surgery in a rabbit model.
  
  
  METHODS
  The superior rectus muscle was recessed in each of 36 eyes from 18 rabbits. One randomly assigned eye in each rabbit was treated with a poloxamer-alginate mixture (PA group); the other eye was treated with a subconjunctival injection of saline (control group). The adhesions between the superior rectus muscle, sclera, and conjunctiva were clinically evaluated by a masked observer at 1 day, 1 week, and 4 weeks after surgery. Inflammation was analyzed by hematoxylin and eosin staining and anti-CD11b staining. Late fibrosis was assessed by the Masson trichrome and α-smooth muscle actin staining. Adhesion, inflammation, and fibrosis were graded on a scale of 0-4.
  
  
  RESULTS
  There was no significant between-group difference in the degree of adhesion at 1 day and 4 weeks after surgery. However, the degree of adhesion in the PA group was lower than that in the control group at postoperative week 1 (P < 0.05). Acute inflammation was similar between the groups (P > 0.05). At postoperative week 4, inflammatory cell infiltration was reduced in the PA group (P = 0.046). A significant between-group difference in late fibrosis at postoperative week 4 was observed through the Masson trichrome (P = 0.024) and α-smooth muscle actin staining (P = 0.025).
  
  
  CONCLUSIONS
  The poloxamer-alginate mixture significantly decreased adhesion after strabismus surgery in a rabbit model. Additional studies are warranted to evaluate the use of this mixture in preventing postoperative adhesions.
authors.affiliation: Department of Ophthalmology, Dong-A University College of Medicine, Busan, Republic of Korea. Electronic address: wyryu@dau.ac.kr.
authors.forename: Won Yeol
authors.initials: WY
authors.lastname: Ryu
authors.forename: Hye Mi
authors.initials: HM
authors.lastname: Jung
authors.forename: Mee Sook
authors.initials: MS
authors.lastname: Roh
authors.forename: Yoon Hyung
authors.initials: YH
authors.lastname: Kwon
authors.forename: Woo Jin
authors.initials: WJ
authors.lastname: Jeung
authors.forename: Woo Chan
authors.initials: WC
authors.lastname: Park
authors.forename: Sae Heun
authors.initials: SH
authors.lastname: Rho
authors.forename: Hee Bae
authors.initials: HB
authors.lastname: Ahn
mesh_terms: D000464:Alginates / Q000008:administration & dosage*; D000818:Animals; D002122:Calcium Chloride / Q000008:administration & dosage; D003229:Conjunctival Diseases / Q000517:prevention & control; D004195:Disease Models, Animal; D005355:Fibrosis / Q000473:pathology / Q000517:prevention & control; D013508:Ophthalmologic Surgical Procedures / Q000009:adverse effects / Q000379:methods; D020442:Poloxamer / Q000008:administration & dosage*; D011183:Postoperative Complications / Q000517:prevention & control*; D011817:Rabbits; D015422:Scleral Diseases / Q000517:prevention & control*; D013285:Strabismus / Q000188:drug therapy / Q000601:surgery*; D013696:Temperature; D000267:Tissue Adhesions / Q000517:prevention & control
chemical_list: D000464:Alginates; D020442:Poloxamer; D002122:Calcium Chloride