title: The transacting factor CBF-A/Hnrnpab binds to the A2RE/RTS element of protamine 2 mRNA and contributes to its translational regulation during mouse spermatogenesis.
abstract: During spermatogenesis, mRNA localization and translation are believed to be regulated in a stage-specific manner. We report here that the Protamine2 (Prm2) mRNA transits through chromatoid bodies of round spermatids and localizes to cytosol of elongating spermatids for translation. The transacting factor CBF-A, also termed Hnrnpab, contributes to temporal regulation of Prm2 translation. We found that CBF-A co-localizes with the Prm2 mRNA during spermatogenesis, directly binding to the A2RE/RTS element in the 3' UTR. Although both p37 and p42 CBF-A isoforms interacted with RTS, they associated with translationally repressed and de-repressed Prm2 mRNA, respectively. Only p42 was found to interact with the 5'cap complex, and to co-sediment with the Prm2 mRNA in polysomes. In CBF-A knockout mice, expression of protamine 2 (PRM2) was reduced and the Prm2 mRNA was prematurely translated in a subset of elongating spermatids. Moreover, a high percentage of sperm from the CBF-A knockout mouse showed abnormal DNA morphology. We suggest that CBF-A plays an important role in spermatogenesis by regulating stage-specific translation of testicular mRNAs.
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Nanaho
authors.initials: N
authors.lastname: Fukuda
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Fukuda
authors.forename: John
authors.initials: J
authors.lastname: Sinnamon
authors.forename: Abrahan
authors.initials: A
authors.lastname: Hernandez-Hernandez
authors.forename: Manizheh
authors.initials: M
authors.lastname: Izadi
authors.forename: Chandrasekhar S
authors.initials: CS
authors.lastname: Raju
authors.forename: Kevin
authors.initials: K
authors.lastname: Czaplinski
authors.forename: Piergiorgio
authors.initials: P
authors.lastname: Percipalle
mesh_terms: D000818:Animals; D023081:CCAAT-Binding Factor / Q000235:genetics* / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D011132:Polyribosomes; D011479:Protamines / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics*; D013737:Testis / Q000378:metabolism
chemical_list: D023081:CCAAT-Binding Factor; C492062:Nfyb protein, mouse; D011479:Protamines; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C068621:protamine 2