title: Autotrophic nitrogen removal from domestic sewage in MBR-CANON system and the biodiversity of functional microbes.
abstract: The feasibility of completely autotrophic nitrogen removal over nitrite (CANON) process for treating domestic sewage was investigated in membrane bioreactor (MBR), for which conventional activated sludge was seeded at ambient temperature. By gradually decreasing hydraulic retention time under the oxygen-limited condition, CANON was successfully started-up for 78 days. Finally the MBR-CANON system was adopted for treating domestic sewage, nitrogen and COD removal achieved to 0.97 kg m(-3) d(-1), 80%, respectively, with the effluent turbidity below 1.0 NTU. DGGE profiles showed a distinct community shift of the functional bacteria after seeded to the reactor, and phylogenetic results indicated the predominance of Nitrosomonas and Candidatus Kuenenia stuttgartiensis for nitrogen removal in the reactor. FISH results showed the predominance of aerobic ammonia oxidizing bacteria (AerAOB) and anaerobic ammonia oxidizing bacteria (AnAOB) in the system, both of whose proportion reduced when treated domestic sewage.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Zhang
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Liang
authors.forename: Yongping
authors.initials: Y
authors.lastname: He
authors.forename: Yulong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D052818:Autotrophic Processes; D001419:Bacteria / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D044822:Biodiversity; D019149:Bioreactors / Q000382:microbiology*; D058645:Denaturing Gradient Gel Electrophoresis; D017404:In Situ Hybridization, Fluorescence; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D009573:Nitrites / Q000378:metabolism*; D009584:Nitrogen / Q000302:isolation & purification*; D012722:Sewage / Q000382:microbiology*; D062065:Wastewater / Q000382:microbiology; D018508:Water Purification
chemical_list: D008567:Membranes, Artificial; D009573:Nitrites; D009584:Nitrogen; D012722:Sewage; D062065:Wastewater
keywords: CANON; Domestic sewage; HRT; Low ammonia; MBR