title: Overexpression of NgAUREO1, the gene coding for aurechrome 1 from Nannochloropsis gaditana, into Saccharomyces cerevisiae leads to a 1.6-fold increase in lipid accumulation.
abstract: Aureochrome-1 (AUREO1) is a transcription factor that is induced by blue light and controls branching of Vaucheria frigida. We have cloned the gene, NgAUREO1, coding for AUREO1 from Nannochloropsis gaditana, and report that the lipid content in recombinant Saccharomyces cerevisiae was 1.6-fold more than in wild-type S. cerevisiae (6.3 % lipid increased to 10 %). Over-expression of AUREO1 in S. cerevisiae up-regulated the expression of acetyl-CoA carboxylase and acyl-CoA:diacylglycerol acyl-transferase but down-regulated the expression of long-chain-acyl CoA synthetase. This enhanced the accumulation of lipid. This study highlights a novel function of AUREO1 and allows a better understanding of the regulation mechanism of fatty acid metabolism.
authors.affiliation: College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, People's Republic of China, huangyijiang@aliyun.com.
authors.forename: Yi-Jiang
authors.initials: YJ
authors.lastname: Huang
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Ming-Gang
authors.initials: MG
authors.lastname: Zheng
authors.forename: Li
authors.initials: L
authors.lastname: Zheng
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Tong
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000096:biosynthesis; D003066:Coenzyme A Ligases / Q000096:biosynthesis; D051048:Diacylglycerol O-Acyltransferase / Q000096:biosynthesis; D015870:Gene Expression; D020869:Gene Expression Profiling; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D058009:Stramenopiles / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D008055:Lipids; D011994:Recombinant Proteins; D014157:Transcription Factors; D051048:Diacylglycerol O-Acyltransferase; D003066:Coenzyme A Ligases; D000103:Acetyl-CoA Carboxylase