title: [Detection of carbapenem-producing Enterobacteriaceae by MALDI-TOF MS].
abstract: OBJECTIVE
  To evaluate a rapid matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) assay in detecting carbapenemase-producing Enterobacteriaceae.
  
  
  METHODS
  Thirty-four carbapenemase-producing Enterobacteriaceae and 50 carbapenem susceptible Enterobacteriaceae clinical isolates were tested. After a 2-hour incubation of bacteria with meropenem, the mixtures were centrifuged and the supernatants analyzed by MALDI-TOF MS.
  
  
  RESULTS
  All spectra of carbapenem susceptible Enterobacteriaceae showed peaks of m/z 384, 406, 428 while those of carbapenemase-producing Enterobacteriaceae m/z 358, 380, 402, 424, 446, 468. And the assay of MALDI-TOF MS was highly consistent with sequencing (P = 1.000).
  
  
  CONCLUSIONS
  MALDI-TOF MS can detect carbapenemase-producing Enterobacteriaceae rapidly and accurately.
authors.affiliation: Department of Laboratory Medicine, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Wang
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: Fan
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.forename: Xin-xin
authors.initials: XX
authors.lastname: Lu
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D015780:Carbapenems / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases; C063912:carbapenemase