title: Study the biocatalyzing effect and mechanism of cellulose acetate immobilized redox mediators technology (CE-RM) on nitrite denitrification.
abstract: The biocatalyzing effect of a novel cellulose acetate immobilized redox mediators technology (CE-RM) on nitrite denitrification process was studied with anthraquinone, 1,8-dichloroanthraquinone, 1,5-dichloroanthraquinone and 1,4,5,8-tetrachloroanthraquinone. The results showed that the immobilized 1,4,5,8-tetrachloroanthraquinone presented the best biocatalyzed effect which increased nitrite denitrification rate to 2.3-fold with 12 mmol/L 1,4,5,8-tetrachloroanthraquinone. The unequal biocatalyzing effect was due to the quantity and position of -Cl substituent in anthraquinone-structure. Moreover, the nitrite denitrification rate was increased with the oxidation reduction potential (ORP) values becoming more negative during the biocatalyzing process. The stabilized ORP value with 12 mmol/L immobilized 1,4,5,8-tetrachloroanthraquinone were 81 mV lower than the control. At the same time, the more OH(-) was produced with the higher nitrite removal rate achieved in the nitrite denitrification process. In addition, a positive linear correlation was found between the nitrite removal reaction constants k [gNO2(-)-N/(gVSS d)] and immobilized 1,4,5,8-tetrachloroanthraquinone concentration (C1,4,5,8-tetrachloroanthraquinone), which was k = 1.8443 C1,4,5,8-tetrachloroanthraquinone + 33.75(R(2) = 0.9411). The initial nitrite concentration of 179 mgNO2(-)-N/L resulted in the maximum nitrite removal rate, which was 6.526[gNO2(-)-N/(gVSS d)]. These results show that the application of cellulose acetate immobilized redox mediators (CE-RM) can be valuable for increasing nitrite denitrification rate.
authors.affiliation: School of Environmental Science and Engineering, Hebei University of Science and Technology, Yuhua East Road 70#, Shijiazhuang, 050018, People's Republic of China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Li
authors.forename: Jianbo
authors.initials: J
authors.lastname: Guo
authors.forename: Jing
authors.initials: J
authors.lastname: Lian
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Xi
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhao
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Liu
authors.forename: Chenxiao
authors.initials: C
authors.lastname: Zhang
authors.forename: Jingliang
authors.initials: J
authors.lastname: Yang
mesh_terms: D000880:Anthraquinones / Q000737:chemistry*; D055162:Biocatalysis; D001673:Biodegradation, Environmental; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D058440:Denitrification; D055664:Electrochemical Techniques; D007700:Kinetics; D059013:Microbial Consortia / Q000502:physiology*; D009573:Nitrites / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D013329:Structure-Activity Relationship; D062065:Wastewater / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000880:Anthraquinones; D002482:Cellulose; D009573:Nitrites; D062065:Wastewater; D014874:Water Pollutants, Chemical; C005062:acetylcellulose