title: Genetic confirmation of the role of sulfopyruvate decarboxylase in coenzyme M biosynthesis in Methanococcus maripaludis.
abstract: Coenzyme M is an essential coenzyme for methanogenesis. The proposed biosynthetic pathway consists of five steps, of which the fourth step is catalyzed by sulfopyruvate decarboxylase (ComDE). Disruption of the gene comE by transposon mutagenesis resulted in a partial coenzyme M auxotroph, which grew poorly in the absence of coenzyme M and retained less than 3% of the wild type level of coenzyme M biosynthesis. Upon coenzyme M addition, normal growth of the mutant was restored. Moreover, complementation of the mutation with the wild type comE gene in trans restored full growth in the absence of coenzyme M. These results confirm that ComE plays an important role in coenzyme M biosynthesis. The inability to yield a complete CoM auxotroph suggests that either the transposon insertion failed to completely inactivate the gene or M. maripaludis possesses a promiscuous activity that partially complemented the mutation.
authors.affiliation: Department of Microbiology, University of Georgia, 541 Biological Science Building, Athens, GA 30602-2605, USA.
authors.forename: Felipe
authors.initials: F
authors.lastname: Sarmiento
authors.forename: Courtney K
authors.initials: CK
authors.lastname: Ellison
authors.forename: William B
authors.initials: WB
authors.lastname: Whitman
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism; D002262:Carboxy-Lyases / Q000235:genetics* / Q000378:metabolism; D004251:DNA Transposable Elements / Q000235:genetics; D019766:Genes, Archaeal; D015080:Mesna / Q000378:metabolism*; D008697:Methane / Q000096:biosynthesis; D017017:Methanococcus / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D019843:Archaeal Proteins; D004251:DNA Transposable Elements; D002262:Carboxy-Lyases; D015080:Mesna; D008697:Methane