title: Differential expression of the metastasis suppressor KAI1 in decidual cells and trophoblast giant cells at the feto-maternal interface.
abstract: Invasion of trophoblasts into maternal uterine tissue is essential for establishing mature feto-maternal circulation. The trophoblast invasion associated with placentation is similar to tumor invasion. In this study, we investigated the role of KAI1, an antimetastasis factor, at the maternal-fetal interface during placentation. Mouse embryos were obtained from gestational days 5.5 (E5.5) to E13.5. Immunohistochemical analysis revealed that KAI1 was expressed on decidual cells around the track made when a fertilized ovum invaded the endometrium, at days E5.5 and E7.5, and on trophoblast giant cells, along the central maternal artery of the placenta at E9.5. KAI1 in trophoblast giant cells was increased at E11.5, and then decreased at E13.5. Furthermore, KAI1 was upregulated during the forskolin-mediated trophoblastic differentiation of BeWo cells. Collectively, these results indicate that KAI1 is differentially expressed in decidual cells and trophoblasts at the maternal-fetal interface, suggesting that KAI1 prevents trophoblast invasion during placentation.
authors.affiliation: Departments of Biochemistry and Cell Biology, School of Medicine; Departments of WCU and BK21 Plus Program, Kyungpook National University, Daegu 700-422, Korea jechoi@knu.ac.kr.
authors.forename: Tae Bon
authors.initials: TB
authors.lastname: Koo
authors.forename: Min-Su
authors.initials: MS
authors.lastname: Han
authors.forename: Yamashita
authors.initials: Y
authors.lastname: Tadashi
authors.forename: Won Joon
authors.initials: WJ
authors.lastname: Seong
authors.forename: Je-Yong
authors.initials: JY
authors.lastname: Choi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003656:Decidua / Q000166:cytology*; D005260:Female; D015726:Giant Cells / Q000378:metabolism*; D007150:Immunohistochemistry; D051861:Kangai-1 Protein / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014327:Trophoblasts / Q000166:cytology*
chemical_list: D051861:Kangai-1 Protein