title: Long-lasting in vivo and in vitro antibacterial ability of nanostructured titania coating incorporated with silver nanoparticles.
abstract: Although titanium (Ti) implants are widely used clinically, implant-associated bacterial infection is still one of the most serious complications in orthopedic surgery. Long-term antibacterial properties and the ability to inhibit biofilm formation are highly desirable to prevent implant associated infection. In this study, a controllable amount of silver (Ag) nanoparticles was incorporated into titanium oxide; or titanium, nanotubes (TiO₂ -NTs). The reliable release and long-term antibacterial function of Ag, in vivo and in vitro, and influence normal bone-implant integration from the Ag released from Ag-incorporated NTs in vivo have been studied to make them useable in clinical practice. In the current study, TiO₂ -NTs loaded with Ag (NT-Ag) exhibited strong antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA, ATCC43300) in vitro for 30 days, and the ability to penetrate the protein layer well. In addition, X-ray examination and 2-[(18)F]-fiuoro-2-deoxy-D-glucose positron emission tomography indicates that NT-Ag show extremely long antibacterial activity in vivo in a rat model. Furthermore, histomorphometric analysis demonstrated that satisfactory bio-integration can be expected. Our results indicate that NT-Ag has both simultaneous antimicrobial and excellent bio-integration properties, make it a promising therapeutic material for orthopedic application.
authors.affiliation: Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Cheng
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Kaifu
authors.initials: K
authors.lastname: Huo
authors.forename: Biao
authors.initials: B
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Xiong
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D019788:Fluorodeoxyglucose F18; D007150:Immunohistochemistry; D020007:Implants, Experimental; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010019:Osteomyelitis / Q000000981:diagnostic imaging / Q000473:pathology; D049268:Positron-Emission Tomography; D011859:Radiography; D017207:Rats, Sprague-Dawley; D012834:Silver / Q000494:pharmacology*; D013977:Tibia / Q000187:drug effects / Q000473:pathology; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D019788:Fluorodeoxyglucose F18; C009495:titanium dioxide; D012834:Silver; D014025:Titanium
keywords: implant-associated infections; long antibacterial activity; rat model; silver nanoparticle; titania nanotubes (TiO2-NTs)