title: Intercellular adhesion molecular-1, Fas, and Fas ligand as diagnostic biomarkers for acute allograft rejection of pancreaticoduodenal transplantation in pigs.
abstract: BACKGROUND
  The early diagnosis of pancreas allograft dysfunction is crucial for the management and long-term survival of transplanted pancreases. We investigated whether intercellular adhesion molecular-1 (ICAM-1), Fas, and Fas ligand (FasL) can be used as novel biomarkers of acute pancreaticoduodenal allograft dysfunction in pigs.
  
  
  METHODS
  Forty outbred landraces were randomly divided into three groups. In the control group (8 pigs), a sham operation was performed but no drugs were administered. In groups 1 and 2 (8 pairs each), pancreaticoduodenal transplantation was performed, with the latter administered immunosuppressive drugs and the former not administered drugs. The expression of ICAM-1, Fas, and FasL mRNA in the peripheral vein blood was assessed by flow cytometry and RT-PCR, pre-transplant and on days 1, 3, 5, and 7 after transplantation. Simultaneously, the levels of glucose, insulin, and glucagon in the serum of the recipients were evaluated. The allograft pancreas tissue was obtained to assess the pathological damage and the expression of Fas and FasL by immunohistochemistry.
  
  
  RESULTS
  On the first 7 days after transplantation, ICAM-1, Fas, and FasL mRNA expression in the blood leukocytes of the recipient increased significantly in groups 1 and 2 compared with the control group (P < 0.01). However, the levels in group 2 were significantly lower than those in group 1 (P < 0.05). Interestingly, the FasL expression increased but the Fas expression decreased gradually in the graft pancreas tissue during the first week after transplantation in both groups 1 and 2 compared with the control group (P < 0.05). The levels of serous glucose, insulin, and glucagon in groups 1 and 2 obviously changed on day 1 after transplantation but returned to normal on day 2. The recipient's pancreas pathological sections did not exhibit any rejection changes on days 1 and 3 after transplantation but showed rejection damage on days 5 and 7.
  
  
  CONCLUSIONS
  ICAM-1, Fas, and FasL were found to be sensitive biomarkers of acute pancreas allograft dysfunction after pancreaticoduodenal transplantation in pigs, and their monitoring could be used to evaluate the effectiveness of the immunosuppression therapy.
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, China.
authors.forename: Gao-hong
authors.initials: GH
authors.lastname: Dong
authors.forename: Xuan-fei
authors.initials: XF
authors.lastname: Li
authors.forename: Jin-zheng
authors.initials: JZ
authors.lastname: Li
authors.forename: Zhao-da
authors.initials: ZD
authors.lastname: Zhang
authors.forename: Wei-ming
authors.initials: WM
authors.lastname: Hu
authors.forename: Yu-hong
authors.initials: YH
authors.lastname: Luo
authors.forename: Zhen-dong
authors.initials: ZD
authors.lastname: Li
authors.forename: Bo-le
authors.initials: BL
authors.lastname: Tian
authors.forename: Man-xi
authors.initials: MX
authors.lastname: He
authors.forename: Xi-Wen
authors.initials: XW
authors.lastname: Zhu
mesh_terms: D064591:Allografts; D000818:Animals; D015415:Biomarkers / Q000097:blood*; D004386:Duodenum / Q000637:transplantation; D053222:Fas Ligand Protein / Q000097:blood*; D005934:Glucagon / Q000097:blood; D006084:Graft Rejection / Q000175:diagnosis* / Q000473:pathology; D007328:Insulin / Q000097:blood; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood*; D007962:Leukocytes / Q000737:chemistry; D010179:Pancreas / Q000473:pathology; D016035:Pancreas Transplantation; D013552:Swine; D019014:fas Receptor / Q000097:blood*
chemical_list: D015415:Biomarkers; D053222:Fas Ligand Protein; D007328:Insulin; D019014:fas Receptor; D018799:Intercellular Adhesion Molecule-1; D005934:Glucagon