title: Noninvasive in vivo monitoring of extracellular vesicles.
abstract: Extracellular vesicles (EVs) including exosomes and microvesicles are nanometer-sized vesicles released by cells to deliver lipids, cellular proteins, mRNAs, and noncoding RNAs, thereby facilitating intercellular communication without direct cell-to-cell contacts. Due to their nanoscale size, EVs have been visualized under microscopy in vitro. We here describe a strategy to label EVs with Gaussia luciferase for noninvasive bioluminescence imaging and monitoring of systemically administered EVs in vivo.
authors.affiliation: Department of Neurology, Neuroscience Center, Massachusetts General Hospital, Boston, MA, USA.
authors.forename: Charles P
authors.initials: CP
authors.lastname: Lai
authors.forename: Bakhos A
authors.initials: BA
authors.lastname: Tannous
authors.forename: Xandra O
authors.initials: XO
authors.lastname: Breakefield
mesh_terms: D000818:Animals; D002458:Cell Fractionation; D033342:Copepoda / Q000201:enzymology; D057809:HEK293 Cells; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D008163:Luminescent Measurements; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D022161:Transport Vesicles / Q000378:metabolism*
chemical_list: D008156:Luciferases