title: Human plasmacytoid dendritic cells sense lymphocytic choriomeningitis virus-infected cells in vitro.
abstract: We previously reported that exosomal transfer of hepatitis C virus (HCV) positive-strand RNA from human Huh-7 hepatoma cells to human plasmacytoid dendritic cells (pDCs) triggers pDC alpha/beta interferon (IFN-α/β) production in a Toll-like receptor 7 (TLR7)-dependent, virus-independent manner. Here we show that human pDCs are also activated by a TLR7-dependent, virus-independent, exosomal RNA transfer mechanism by human and mouse hepatoma and nonhepatoma cells that replicate the negative-strand lymphocytic choriomeningitis virus (LCMV).
authors.affiliation: Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California, USA.
authors.forename: S F
authors.initials: SF
authors.lastname: Wieland
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: B
authors.initials: B
authors.lastname: Boyd
authors.forename: C
authors.initials: C
authors.lastname: Whitten-Bauer
authors.forename: N
authors.initials: N
authors.lastname: Ngo
authors.forename: J-C
authors.initials: JC
authors.lastname: de la Torre
authors.forename: F V
authors.initials: FV
authors.lastname: Chisari
mesh_terms: D003713:Dendritic Cells / Q000166:cytology / Q000821:virology*; D006801:Humans; D066298:In Vitro Techniques; D008217:Lymphocytic choriomeningitis virus / Q000302:isolation & purification*