title: Sperm impairments in adult vesper mice (Calomys laucha) caused by in utero exposure to bisphenol A.
abstract: This study aimed to evaluate the effects of in utero administration of bisphenol A (BPA) on semen parameters of vesper mice. Sixty female Calomys laucha were divided into six groups and received by gavage during gestation the following substances: Water (negative control), Olive Oil (vehicle control), Diethylstilbestrol (DES - positive control - 6.5 μg kg(-1) bw) and BPA (40, 80 and 200 μg kg(-1) bw). Male offspring were euthanised at 70 days of age, and sperm parameters were analysed. BPA reduced normal sperm morphology (water = 96.1 ± 0.65; BPA200 = 96.8 ± 2.3%), sperm membrane integrity (water = 88.8 ± 1,65; BPA200 = 70.6 ± 4,15%), sperm motility (water = 87.5 ± 1.71; BPA200 = 51.3 ±9.9%) and in vitro penetration rates (water = 55.0 ± 7.14; BPA200 = 7.47 ±2.96%), but it did not affect body weight, anogenital distance, sperm DNA integrity and acrosome integrity. In conclusion, in utero exposure to BPA caused a reduction in sperm parameters of adult C. laucha. Natural mating studies should be conducted to verify the effects of BPA on fertility of the animals.
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Rio Grande (FURG), Rio Grande, RS, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: Vilela
authors.forename: A
authors.initials: A
authors.lastname: Hartmann
authors.forename: E F
authors.initials: EF
authors.lastname: Silva
authors.forename: T
authors.initials: T
authors.lastname: Cardoso
authors.forename: C D
authors.initials: CD
authors.lastname: Corcini
authors.forename: A S
authors.initials: AS
authors.lastname: Varela-Junior
authors.forename: P E
authors.initials: PE
authors.lastname: Martinez
authors.forename: E P
authors.initials: EP
authors.lastname: Colares
mesh_terms: D000818:Animals; D003411:Arvicolinae; D001559:Benzhydryl Compounds / Q000008:administration & dosage / Q000633:toxicity*; D052244:Endocrine Disruptors / Q000633:toxicity*; D005260:Female; D005298:Fertility / Q000187:drug effects; D008297:Male; D010636:Phenols / Q000008:administration & dosage / Q000633:toxicity*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000473:pathology* / Q000503:physiopathology*; D013081:Sperm Motility / Q000187:drug effects; D013084:Sperm-Ovum Interactions / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol; Calomys; endocrine disruptor; reproduction; semen