title: Detection of Candida albicans Sap2 in cancer patient serum samples by an indirect competitive enzyme-linked immunosorbent assay for the diagnosis of candidiasis.
abstract: BACKGROUND
  The secreted aspartyl proteinases 2 (Sap2) of Candida albicans (C. albicans) is a potential marker of candididasis. It is a virulence factor associated with adherence and tissue invasion.
  
  
  OBJECTIVE
  In order to detect Sap2 in clinical sera, we developed an indirect competitive enzyme-linked immunosorbent assay (ELISA).
  
  
  METHODS
  Polyclonal antibodies were produced for Sap2 by injecting Sap2 into a New Zealand White inbred rabbit. They could be used at a dilution exceeding 1:1200 in an indirect ELISA, and detected Sap2 concentration up to 1 ng/mL.
  
  
  RESULTS
  Of the 286 cancer serum samples tested, 16.8% were found as candidiasis. The test was simple and economical to perform and had a level of sensitivity for detection of low-titer positive sera; thus, it may be proven to be of value in epidemiological studies on candidiasis.
authors.affiliation: School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province, P.R. China, .
authors.forename: Yicun
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: J U
authors.initials: JU
authors.lastname: Zhi Gang
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Liu
authors.forename: Shuai
authors.initials: S
authors.lastname: Dong
authors.forename: Li
authors.initials: L
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000908:Antibodies, Fungal; D016282:Aspartic Acid Endopeptidases / Q000097:blood*; D015415:Biomarkers / Q000097:blood*; D002177:Candidiasis / Q000175:diagnosis*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D003365:Costs and Cost Analysis; D003955:Diagnostic Tests, Routine / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005656:Fungal Proteins / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000150:complications; D011817:Rabbits; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry
chemical_list: D000908:Antibodies, Fungal; D015415:Biomarkers; D005656:Fungal Proteins; D016282:Aspartic Acid Endopeptidases; C090753:SAP2 protein, Candida