title: An improved fluorogenic NAD(P)+ detection method using 2-acetylbenzofuran: its origin and application.
abstract: We have developed a fluorogenic NAD(P)(+) detection method using 2-acetylbenzofuran. The reaction of NAD(P)(+) with 2-acetylbenzofuran produced a fluorescent product, allowing the highly-sensitive and quick detection of NAD(P)(+). This method was successfully applied to the detection of P450 substrates in the microtiter-plate format.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aza-aoba, Aramaki, Aoba-ku, Sendai 980-8578, Japan. nkanoh@m.tohoku.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Moriya
authors.forename: Ayano
authors.initials: A
authors.lastname: Kawamata
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Iwabuchi
authors.forename: Naoki
authors.initials: N
authors.lastname: Kanoh
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D001572:Benzofurans / Q000737:chemistry*; D019475:Camphor 5-Monooxygenase / Q000737:chemistry; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D007659:Ketones / Q000737:chemistry; D009249:NADP / Q000737:chemistry*; D009251:NADPH-Ferrihemoprotein Reductase / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D001572:Benzofurans; D005456:Fluorescent Dyes; D007659:Ketones; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; C469295:2-acetylbenzofuran; D019475:Camphor 5-Monooxygenase; D009251:NADPH-Ferrihemoprotein Reductase; C052423:flavocytochrome P450 BM3 monoxygenases