title: Enzymatically catalyzed synthesis of anti-blooming agent 1,3-dibehenoyl-2-oleoyl glycerol in a solvent-free system: optimization by response surface methodology.
abstract: Products rich in 1,3-dibehenoyl-2-oleoyl glycerol (BOB) triglyceride (TAG) were produced by enzymatic interesterification of high oleic acid sunflower oil (HOSO) and behenic acid methyl ester (BME) by 1,3-regiospecific lipase Lipozyme RM IM in a solvent-free system. The impact factors of enzyme load, substrate molar ratio of BME to HOSO (BME/HOSO), reaction time, reaction temperature, and pre-equilibration water activity of the enzyme on BOB content and BME conversions were investigated by single-factor experiments and then optimized using the response surface methodology (RSM). The optimum conditions were as follows: reaction temperature, 72 °C; reaction time, 7.99 h; substrate molar ratio, 2.5:1; enzyme load, 10%; and pre-equilibration water activities of the enzyme, 0.28. The results from the experiments conducted according to the predicted optimal conditions were as follows: the content of BOB was 32.76%, and the conversion of BME was 65.16%. The experimental values agreed with the predicted values, which verified the sufficiency of the quadratic regression models. After purification under the optimal short-range molecular distillation and two-step solvent fractionation, the content of BOB in the target product can reach 77.14%, indicating the great potential for industrial production of the anti-blooming agent.
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University , 1800 Lihu Road, Wuxi, Jiangsu 214122, People's Republic of China.
authors.forename: Zong
authors.initials: Z
authors.lastname: Meng
authors.forename: Wen-Xin
authors.initials: WX
authors.lastname: Geng
authors.forename: Jin-Wei
authors.initials: JW
authors.lastname: Li
authors.forename: Zhao-Qi
authors.initials: ZQ
authors.lastname: Yang
authors.forename: Jiang
authors.initials: J
authors.lastname: Jiang
authors.forename: Xing-Guo
authors.initials: XG
authors.lastname: Wang
authors.forename: Yuan-Fa
authors.initials: YF
authors.lastname: Liu
mesh_terms: D055162:Biocatalysis; D004951:Esterification; D005223:Fats / Q000737:chemistry*; D005990:Glycerol / Q000737:chemistry*; D007700:Kinetics; D008049:Lipase / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry; D000074242:Sunflower Oil
chemical_list: D005223:Fats; D010938:Plant Oils; D000074242:Sunflower Oil; C093666:Lipozyme; D008049:Lipase; D005990:Glycerol