title: Rhizosphere competent microbial consortium mediates rapid changes in phenolic profiles in chickpea during Sclerotium rolfsii infection.
abstract: The present study was carried out with the aim of evaluating the effectiveness and potentiality of three compatible rhizosphere microbes, viz., fluorescent Pseudomonas aeruginosa (PHU094), Trichoderma harzianum (THU0816) and Mesorhizobium sp. (RL091), in promoting plant growth and mobilizing phenolic acid biosynthesis in chickpea under challenge of Sclerotium rolfsii. The microbes were applied as seed coating in different combinations in two experimental sets and the pathogen was inoculated after 25 days of sowing in one set. Results revealed that microbe application led to higher growth in chickpea particularly in the triple microbe combination compared to their individual treatments and control. Similarly, pathogen challenged plants accumulated higher amount of phenolic compounds both at the site of attack of the pathogen i.e. collar region as well as leaves compared to unchallenged plants. All the bioagents were found to trigger the level of phenolic compounds at collar region in varying degrees as compared to the healthy control (A). However, the most effective treatment was D7 (combined application of PHU094, THU0816 and RL091 with pathogen challenge) among all the treatments. Shikimic acid was maximally induced amongst all the phenolic compounds. In leaves also, the most effective treatment was D7 where shikimic acid, t-chlorogenic acid, ferulic acid, myricetin, quercetin and syringic acid were produced in higher amounts as compared to treatment B where the plants were challenged only with the pathogen.
authors.affiliation: Department of Botany, Banaras Hindu University, Varanasi 221005, India. Electronic address: bhuaks29@gmail.com.
authors.forename: Akanksha
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Botany, Banaras Hindu University, Varanasi 221005, India. Electronic address: akansha007@rediffmail.com.
authors.forename: Akansha
authors.initials: A
authors.lastname: Jain
authors.affiliation: Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, India. Electronic address: birinchi_ks@yahoo.com.
authors.forename: Birinchi Kumar
authors.initials: BK
authors.lastname: Sarma
authors.affiliation: Department of Botany, Banaras Hindu University, Varanasi 221005, India. Electronic address: upadhyay_bhu@yahoo.co.uk.
authors.forename: Ram S
authors.initials: RS
authors.lastname: Upadhyay
authors.affiliation: Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, India. Electronic address: hbs1@rediffmail.com.
authors.forename: Harikesh Bahadur
authors.initials: HB
authors.lastname: Singh
mesh_terms: D001487:Basidiomycota / Q000254:growth & development*; D029863:Cicer / Q000737:chemistry* / Q000254:growth & development / Q000382:microbiology*; D059128:Mesorhizobium / Q000254:growth & development / Q000378:metabolism; D059013:Microbial Consortia; D010636:Phenols / Q000032:analysis*; D063245:Plant Development; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism; D012988:Soil Microbiology; D014242:Trichoderma / Q000254:growth & development / Q000378:metabolism
chemical_list: D010636:Phenols
keywords: HPLC; Microbial consortium; Phenylpropanoid pathway; Sclerotium rolfsii; Shikimic acid