title: Construction and rapid testing of synthetic and modified toxin gene sequences CryIA (b&c) by expression in maize endosperm culture.
abstract: The synthesis of two modified genes, Cry IA(b) and CryIA(c), each consisting of 1845 bp, is described in detail. The genes were synthesized using an improved PCR procedure based on recursive principles. The synthetic CryIA(c) gene was put under the control of a maize ubiquitin promoter. This construct was tested in a maize endosperm-derived suspension culture system. The use of maize endosperm culture as a quick and efficient system to test the activity of synthetic genes is described.
authors.affiliation: Department of Biochemistry, University of Ottawa, 40 Marie Curie Private, K1N 6N5, Ottawa, Ontario, Canada.
authors.forename: R
authors.initials: R
authors.lastname: Sardana
authors.forename: S
authors.initials: S
authors.lastname: Dukiandjiev
authors.forename: M
authors.initials: M
authors.lastname: Giband
authors.forename: X
authors.initials: X
authors.lastname: Cheng
authors.forename: K
authors.initials: K
authors.lastname: Cowan
authors.forename: C
authors.initials: C
authors.lastname: Sauder
authors.forename: I
authors.initials: I
authors.lastname: Altosaar