title: Comparison between placental gene expression of 11β-hydroxysteroid dehydrogenases and infantile growth at 10 months of age.
abstract: OBJECTIVE
  The local expression of two isoenzymes of 11β-hydroxysteroid dehydrogenase, type 1 (11βHSD-1) and type 2 (11βHSD-2), regulates the access of glucocorticoid hormones to their target cells. Reports on the association between the placental expression of 11βHSD and infantile growth are limited. The aim of the present study was to investigate if the placental gene expression of 11βHSD affects infantile growth at 10 months of age.
  
  
  METHODS
  Placentas and umbilical venous cord blood were obtained from 42 singleton cases of cesarean deliveries between 31 and 40 weeks of gestation at Hamamatsu University Hospital between March 2009 and June 2010. The gene expression of both 11βHSD-1 and 11βHSD-2 was measured by quantitative reverse transcription polymerase chain reaction. Adiponectin and leptin levels in umbilical cord blood were measured using enzyme-linked immunoassay.
  
  
  RESULTS
  11βHSD-1 and 11βHSD-2 gene expression in human placentas did not correlate with bodyweight or the ponderal index (PI) at 10 months of age, whereas the gene expression of 11βHSD-1, but not 11βHSD-2, correlated with birthweight as well as PI at birth. Adiponectin levels in umbilical cord blood significantly correlated with the placental gene expression of 11βHSD-1 as well as bodyweight and PI at 10 months of age, although no direct correlation was observed between them.
  
  
  CONCLUSIONS
  No direct correlation was observed between the placental gene expression of 11βHSD and infantile growth at 10 months of age. However, the placental gene expression of 11βHSD-1 may be indirectly connected with infantile growth via adiponectin-associated metabolic regulation represented by adiponectin levels in umbilical cord blood.
authors.affiliation: Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Hamamatsu, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Muramatsu-Kato
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Itoh
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kobayashi-Kohmura
authors.forename: Hirotake
authors.initials: H
authors.lastname: Murakami
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Uchida
authors.forename: Kazunao
authors.initials: K
authors.lastname: Suzuki
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sugihara
authors.forename: Naohiro
authors.initials: N
authors.lastname: Kanayama
authors.forename: Kenji J
authors.initials: KJ
authors.lastname: Tsuchiya
authors.forename: Nori
authors.initials: N
authors.lastname: Takei
mesh_terms: D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000235:genetics*; D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2 / Q000235:genetics*; D052242:Adiponectin / Q000097:blood; D000328:Adult; D001835:Body Weight; D002657:Child Development; D005260:Female; D005312:Fetal Blood / Q000378:metabolism; D015870:Gene Expression; D005865:Gestational Age; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007223:Infant; D020738:Leptin / Q000097:blood; D008875:Middle Aged; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism*; D013997:Time Factors; D055815:Young Adult
chemical_list: D052242:Adiponectin; D020738:Leptin; D012333:RNA, Messenger; D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1; D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2; D006854:Hydrocortisone
keywords: glucocorticoid; growth; infant; placenta; pregnancy; programming.