title: Improvement of anti-inflammatory and anti-angiogenic activity of berberine by novel rapid dissolving nanoemulsifying technique.
abstract: Berberine, an isoquinoline alkaloid, has wide biological and pharmacological actions. Despite the promising pharmacological effects and safety of berberine, poor oral absorption due to its extremely low aqueous solubility results in poor oral systemic bioavailability. This limits its clinical usage. This study describes the development and characterization of self-nanoemulsifying drug delivery system (SNEDDS) of berberine in liquid as well as solid form with improved solubility, dissolution and in vivo therapeutic efficacy. The SNEDDS of berberine were prepared using Acrysol K-150, Capmul MCM and polyethylene glycol 400. The formulations were characterized for various in vitro physicochemical characteristics. In vivo efficacy was evaluated in acetic acid induced inflammatory bowel model in rats. Anti-angiogenic activity of the developed SNEDDS of berberine was studied using chick chorioallantoic membrane assay. SNEDDS of berberine rapidly formed nanoemulsions with globule size of 17-45 nm. The in vitro rate and extent of release of berberine from SNEDDS was significantly higher than berberine alone. Chick chorioallantoic membrane assay revealed potent anti-angiogenic activity of SNEDDS of berberine. These studies demonstrate that the SNEDDS of berberine is a promising strategy for improving its therapeutic efficacy and have potential application in the treatment of chronic inflammatory conditions and cancer.
authors.affiliation: Department of Pharmaceutics, STES's Sinhgad Institute of Pharmacy, Pune, India. Electronic address: swatipund@gmail.com.
authors.forename: Swati
authors.initials: S
authors.lastname: Pund
authors.affiliation: Department of Pharmaceutics, STES's Sinhgad Institute of Pharmacy, Pune, India.
authors.forename: Ganesh
authors.initials: G
authors.lastname: Borade
authors.affiliation: Department of Pharmaceutics, STES's Sinhgad Institute of Pharmacy, Pune, India.
authors.forename: Ganesh
authors.initials: G
authors.lastname: Rasve
mesh_terms: D020533:Angiogenesis Inhibitors / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D001599:Berberine / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D001682:Biological Availability; D002642:Chick Embryo; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D004355:Drug Stability; D004655:Emulsions; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy; D015212:Inflammatory Bowel Diseases / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D053758:Nanoparticles; D009369:Neoplasms / Q000188:drug therapy; D018919:Neovascularization, Physiologic / Q000187:drug effects; D010316:Particle Size; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility
chemical_list: D020533:Angiogenesis Inhibitors; D000893:Anti-Inflammatory Agents; D004337:Drug Carriers; D004655:Emulsions; D010936:Plant Extracts; D001599:Berberine
keywords: Anti-angiogenic; Anti-inflammatory; Berberine; Dissolution; SNEDDS