title: Simultaneous determination of aflatoxin M1, ochratoxin A, zearalenone and α-zearalenol in milk by UHPLC-MS/MS.
abstract: In this study, a sensitive and rapid method has been developed for the simultaneous determination of aflatoxin M1, ochratoxin A, zearalenone and α-zearalenol in milk by ultra high performance liquid chromatography combined with electrospray ionisation triple quadrupole tandem mass spectrometry (UHPLC-ESI-MS/MS). The milk samples were purified using Oasis HLB cartridge. The matrix effects were evaluated by determining the signal suppression-enhancement (SSE) and corrected by external matrix-matched calibration. The limits of quantity (LOQ) of the mycotoxins were in the range of 0.003-0.015μgkg(-1). The high correlation coefficients (R(2)⩾0.996) were obtained in the range of 0.01-1.00μgkg(-1) of the mycotoxins, along with good recovery (87.0-109%), repeatability (3.4-9.9%) and intra-laboratory reproducibility (4.0-9.9%) at the concentrations of 0.025, 0.1 and 0.5μgkg(-1). The detected rates of the mycotoxins were from 16.7% to 96.7% in raw milk, liquid milk and milk powder samples collected from the dairy farms and supermarkets in Beijing. The method proposed is suitable for the simultaneous determination of aflatoxin M1, ochratoxin A, zearalenone, and α-zearalenol, and could be performed for analysing the mycotoxins in milk.
authors.affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China; Ministry of Agriculture - Milk and Dairy Product Inspection Center (Beijing), Beijing 100193, PR China.
authors.forename: L C
authors.initials: LC
authors.lastname: Huang
authors.forename: N
authors.initials: N
authors.lastname: Zheng
authors.forename: B Q
authors.initials: BQ
authors.lastname: Zheng
authors.forename: F
authors.initials: F
authors.lastname: Wen
authors.forename: J B
authors.initials: JB
authors.lastname: Cheng
authors.forename: R W
authors.initials: RW
authors.lastname: Han
authors.forename: X M
authors.initials: XM
authors.lastname: Xu
authors.forename: S L
authors.initials: SL
authors.lastname: Li
authors.forename: J Q
authors.initials: JQ
authors.lastname: Wang
mesh_terms: D016607:Aflatoxin M1 / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D009793:Ochratoxins / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D015025:Zearalenone / Q000032:analysis*; D015029:Zeranol / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D009793:Ochratoxins; C029659:zearalenol; C025589:ochratoxin A; D015025:Zearalenone; D016607:Aflatoxin M1; D015029:Zeranol
keywords: Matrix effect; Matrix-matched calibration curve; Milk; Mycotoxin; UHPLC–MS/MS