title: Effect of oilseed rape genotype on the spontaneous hybridization rate with a weedy species:an assessment of transgene dispersal.
abstract: Spontaneous outcrossing of different malesterile rapeseed lines and transgenic hybrids with a population of a weedy species, Raphanus raphanistrum L., has led to the harvest of numerous seeds showing a size dimorphism. Flow cytometry analysis correlated with chromosome counts showed that all of the large seeds belonged to rapeseed, whereas the small seeds were a mixture of mostly interspecific triploid hybrids, with some trigenomic amphidiploids, diploid and haploid rapeseed plants. Significant differences were revealed between the rapeseed lines and transgenic hybrids in their ability to form interspecific hybrids with Raphanus raphanistrum under natural conditions. Resistance to the herbicide Basta was properly expressed in the triploid and amphidiploid hybrids. Low male fertility of the interspecific triploid hybrids was not correlated with seed set in the subsequent generation.
authors.affiliation: INRA Station d'amélioration des plantes, BP 29, 35650, Le Rheu, France.
authors.forename: A
authors.initials: A
authors.lastname: Baranger
authors.forename: A M
authors.initials: AM
authors.lastname: Chèvre
authors.forename: F
authors.initials: F
authors.lastname: Eber
authors.forename: M
authors.initials: M
authors.lastname: Renard