title: Peripheral blood natural killer cells activation status determined by CD69 upregulation predicts implantation outcome in IVF.
abstract: OBJECTIVE
  NK lymphocytes play critical yet poorly defined role in implantation and during development in early pregnancy.
  
  
  METHODS
  Recently, we showed that the proportion of NK that expressed CD69⁺ after incubation with K562 (CD69(stim)) cells reflected the NK population excitation potential. In the present study, we investigate the significance of NK activation levels in predicting embryo implantation.
  
  
  RESULTS
  A qualitative analysis of values distribution in two groups showed that 25/33 (75.8%) women who became pregnant had CD69(stim) levels that were >30 but <60% (conditionally normal zone). In contrast, CD69(stim) levels in patients who failed to become pregnant were either elevated, as in 10/51 (19.6%), or reduced, as in 20/51 (39.2%) of the patients. Accentuated CD69(stim) levels were predictive for implantation failure, extremely significant for decreased (OR 6.9, p=0.0004) and not quite significant for increased CD69(stim) levels (OR 3.9, p=0.062). Accordingly, conditionally normal CD69(stim) levels were favourable for implantation (OR 4.46, p=0.0032).
  
  
  CONCLUSIONS
  We confirm that actual peripheral blood natural killer cells activation status have an influence on embryo implantation. We showed that exactly normal NK cell activity predicting successful implantation.
authors.affiliation: Laboratory of Immunology, Institute of Pediatrics, Obstetrics and Gynecology, National Academy of Medical Sciences of Ukraine, Kiev, Ukraine. Electronic address: boris_donskoy@ukr.net.
authors.forename: Boris V
authors.initials: BV
authors.lastname: Dons'koi
authors.affiliation: Laboratory of Immunology, Institute of Pediatrics, Obstetrics and Gynecology, National Academy of Medical Sciences of Ukraine, Kiev, Ukraine.
authors.forename: Viktor P
authors.initials: VP
authors.lastname: Chernyshov
authors.affiliation: Institute of Reproductive Medicine, Kiev, Ukraine.
authors.forename: Vera Y
authors.initials: VY
authors.lastname: Sirenko
authors.affiliation: Institute of Genetic of Reproduction, Kiev, Ukraine.
authors.forename: Galina V
authors.initials: GV
authors.lastname: Strelko
authors.affiliation: Laboratory of Immunology, Institute of Pediatrics, Obstetrics and Gynecology, National Academy of Medical Sciences of Ukraine, Kiev, Ukraine.
authors.forename: Dariia V
authors.initials: DV
authors.lastname: Osypchuk
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D001773:Blood Cells / Q000276:immunology*; D010064:Embryo Implantation / Q000276:immunology; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007246:Infertility / Q000175:diagnosis* / Q000628:therapy*; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000276:immunology*; D037181:Lectins, C-Type / Q000378:metabolism; D008213:Lymphocyte Activation; D011247:Pregnancy / Q000276:immunology; D011256:Pregnancy Outcome; D017211:Treatment Failure; D015854:Up-Regulation; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D037181:Lectins, C-Type; D000099192:CD69 Antigens
keywords: CD69 expression; Embryo implantation; NK activity; NK stimulation; Natural killer lymphocytes