title: Chimerism of allogeneic mesenchymal cells in bone marrow, liver, and spleen after mesenchymal stem cells infusion.
abstract: Although an infusion of culture-expanded MSCs is applied in clinic to improve results of HSCs transplantation and for a treatment of musculoskeletal disorders, homing, and engraftment potential of culture-expanded MSC in humans is still obscure. We report two female patients who received allogeneic BM transplantation as a treatment of hematological diseases and a transplantation of MSCs from third-party male donors. Both patients died within one yr of infectious complications. Specimens of paraffin-embedded blocks of tissues from transplanted patients were taken. The aim of the study was to estimate possible homing and engraftment of allogeneic BM-derived MSCs in some tissues/organs of recipient. Sensitive real-time quantitative PCR analysis was applied with SRY gene as a target. MSC chimerism was found in BM, liver, and spleen of both patients. We conclude that sensitive RQ-PCR analysis is acceptable for low-level chimerism evaluation even in paraffin-embedded tissue specimens.
authors.affiliation: Belarusian Research Center for Pediatric Oncology, Hematology and Immunology, Minsk, Belarus.
authors.forename: Alexander
authors.initials: A
authors.lastname: Meleshko
authors.forename: Irina
authors.initials: I
authors.lastname: Prakharenia
authors.forename: Semen
authors.initials: S
authors.lastname: Kletski
authors.forename: Yanina
authors.initials: Y
authors.lastname: Isaikina
mesh_terms: D000293:Adolescent; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D002648:Child; D046528:Chimerism; D005260:Female; D006801:Humans; D008099:Liver / Q000166:cytology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010232:Paraffin / Q000737:chemistry; D060888:Real-Time Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology; D014184:Transplantation, Homologous
chemical_list: D010232:Paraffin
keywords: bone marrow transplantation; chimerism; mesenchymal stem cells; mesenchymal stem cells homing