title: Ultraviolet absorption spectroscopy of peptides.
abstract: UV absorption spectroscopy is commonly used with peptides for determining concentration and enzyme activity, but high-resolution UV spectra can also provide information on peptide secondary and tertiary structure and association behavior. New developments using temperature- and cation-dependent high-resolution second derivative absorption methods can also provide information concerning peptide dynamics. Data from several low-resolution spectroscopic techniques, including UV absorption, can be combined to generate an overall picture of peptide structure as a function of environmental conditions.
authors.affiliation: Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS, USA.
authors.forename: Mangala R
authors.initials: MR
authors.lastname: Liyanage
authors.forename: Kunal
authors.initials: K
authors.lastname: Bakshi
authors.forename: David B
authors.initials: DB
authors.lastname: Volkin
authors.forename: C Russell
authors.initials: CR
authors.lastname: Middaugh
mesh_terms: D000042:Absorption; D003627:Data Interpretation, Statistical; D007700:Kinetics; D010455:Peptides / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013696:Temperature
chemical_list: D010455:Peptides