title: Increased histone H3 phosphorylation in neurons in specific brain structures after induction of status epilepticus in mice.
abstract: Status epilepticus (SE) induces pathological and morphological changes in the brain. Recently, it has become clear that excessive neuronal excitation, stress and drug abuse induce chromatin remodeling in neurons, thereby altering gene expression. Chromatin remodeling is a key mechanism of epigenetic gene regulation. Histone H3 phosphorylation is frequently used as a marker of chromatin remodeling and is closely related to the upregulation of mRNA transcription. In the present study, we analyzed H3 phosphorylation levels in vivo using immunohistochemistry in the brains of mice with pilocarpine-induced SE. A substantial increase in H3 phosphorylation was detected in neurons in specific brain structures. Increased H3 phosphorylation was dependent on neuronal excitation. In particular, a robust upregulation of H3 phosphorylation was detected in the caudate putamen, and there was a gradient of phosphorylated H3(+) (PH3(+)) neurons along the medio-lateral axis. After unilateral ablation of dopaminergic neurons in the substantia nigra by injection of 6-hydroxydopamine, the distribution of PH3(+) neurons changed in the caudate putamen. Moreover, our histological analysis suggested that, in addition to the well-known MSK1 (mitogen and stress-activated kinase)/H3 phosphorylation/c-fos pathway, other signaling pathways were also activated. Together, our findings suggest that a number of genes involved in the pathology of epileptogenesis are upregulated in PH3(+) brain regions, and that H3 phosphorylation is a suitable indicator of strong neuronal excitation.
authors.affiliation: Department of Anatomy and Cell Science, Kansai Medical University, Hirakata, Osaka, Japan.
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Mori
authors.forename: Taketoshi
authors.initials: T
authors.lastname: Wakabayashi
authors.forename: Haruyuki
authors.initials: H
authors.lastname: Ogawa
authors.forename: Yukie
authors.initials: Y
authors.lastname: Hirahara
authors.forename: Taro
authors.initials: T
authors.lastname: Koike
authors.forename: Hisao
authors.initials: H
authors.lastname: Yamada
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D006657:Histones / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009474:Neurons / Q000378:metabolism*; D016627:Oxidopamine / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D038744:Ribosomal Protein S6 Kinases, 90-kDa / Q000378:metabolism
chemical_list: D006657:Histones; D016627:Oxidopamine; D038744:Ribosomal Protein S6 Kinases, 90-kDa; C116919:mitogen and stress-activated protein kinase 1