title: Clinical application of a molecular method based on real time RT-PCR for detection of influenza A(H1N1)v virus.
abstract: Given the new diagnostic need following the pandemic caused by the A(H1N1)v virus, we evaluated the performance characteristics of Xpert® Flu assay (Cepheid). The overall sensitivity and specificity were 65.6% and 92.8%, respectively. Sensitivity and specificity for A(H1N1)v virus were 85.7% and 94.9%, respectively, and therefore the Xpert® Flu assay is suitable for a rapid diagnosis in critically ill patients where diagnosis is crucial for clinical management and for an appropriate public health response.
authors.affiliation: Unit of Clinical Microbiology, Regional Reference Centre for Microbiological Emergencies (CRREM), St. Orsola-Malpighi University Hospital, Bologna, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Pierro
authors.forename: Paolo
authors.initials: P
authors.lastname: Gaibani
authors.forename: Giada
authors.initials: G
authors.lastname: Rossini
authors.forename: Maria Paola
authors.initials: MP
authors.lastname: Landini
authors.forename: Vittorio
authors.initials: V
authors.lastname: Sambri
mesh_terms: D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D012367:RNA, Viral