title: Selenium-enriched Agaricus bisporus increases expression and activity of glutathione peroxidase-1 and expression of glutathione peroxidase-2 in rat colon.
abstract: The effect of dietary supplementation with Se-enriched Agaricus bisporus on cytosolic gluthathione peroxidase-1 (GPx-1), gastrointestinal specific glutathione peroxidase-2 (GPx-2), thioredoxin reductase-1 (TrxR-1) and selenoprotein P (SeP) mRNA expression and GPx-1 enzyme activity in rat colon was examined. Rats were fed for 5weeks with control diet (0.15μg Se/g feed) or Se-enriched diet fortified with selenised mushroom (1μg Se/g feed). The mRNA expression levels were found to be significantly (P<0.01) up-regulated by 1.65-fold and 2.3-fold for GPx-1 and GPx-2, respectively, but were not significantly different for TrxR-1 and SeP between the 2 diet treatments. The up-regulation of GPx-1 mRNA expression was consistent with GPX-1 activity level, which was significantly (P<0.05) increased by 1.77-fold in rats fed with the Se-enriched diet compared to the control diet. The results showed that selenised A. bisporus can positively increase GPx-1 and GPx-2 gene expression and GPx-1 enzyme activity in rat colon.
authors.affiliation: Department of Agriculture and Food Systems, Melbourne School of Land & Environment, The University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Tebo
authors.initials: T
authors.lastname: Maseko
authors.forename: Kate
authors.initials: K
authors.lastname: Howell
authors.forename: Frank R
authors.initials: FR
authors.lastname: Dunshea
authors.forename: Ken
authors.initials: K
authors.lastname: Ng
mesh_terms: D000364:Agaricus / Q000737:chemistry / Q000378:metabolism*; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D003106:Colon / Q000201:enzymology*; D019587:Dietary Supplements / Q000032:analysis; D005979:Glutathione Peroxidase / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000032:analysis / Q000378:metabolism*; D015854:Up-Regulation; D000094765:Glutathione Peroxidase GPX1
chemical_list: D005979:Glutathione Peroxidase; C525844:glutathione peroxidase 2, rat; D012643:Selenium; D000094765:Glutathione Peroxidase GPX1
keywords: Agaricus bisporus; Colon cancer; Glutathione peroxidase; Se-enriched mushroom; Selenoprotein P; Selenoproteins; Thioredoxin reductase