title: Regulation of transcription of the Saccharomyces cerevisiae CYC1 gene: Identification of a DNA region involved in heme control.
abstract: A Saccharomyces cerevisiae mutant (hem1 cycl-1) was transformed with plasmids bearing a chromosomal centromer (CEN3) and a 2 μm DNA replication origin. In one of the plasmids a functional CYC1 gene was present, in a second plasmid an XhoI fragment located between bases -245 and -678 upstream from the translation initiation codon had been deleted, in a third plasmid this region had been inverted. Results of hybridization experiments carried out with mRNA isolated from heme-deficient and heme-containing transformants indicated that heme controls transcription of the CYC1 gene and that DNA sequences located within the upstream XhoI fragment are involved in activation of the gene by heme.
authors.affiliation: Institut für Allgemeine Biochemie der Universität Wien, Ludwig Boltzmann-Forschungsstelle für Biochemie, Währinger Str. 38, A-1090, Wien, Austria.
authors.forename: R
authors.initials: R
authors.lastname: Gudenus
authors.forename: A
authors.initials: A
authors.lastname: Spence
authors.forename: A
authors.initials: A
authors.lastname: Hartig
authors.forename: M
authors.initials: M
authors.lastname: Smith
authors.forename: H
authors.initials: H
authors.lastname: Ruis