title: Comparison of RAPD and RFLP markers for mapping F2 generations in maize (Zea mays L.).
abstract: The F2 generations from two maize crosses were used to compare the ability of RAPD and RFLP marker systems to create a genetic linkage map. Both RFLPs and RAPDs were shown to provide Mendelian-type markers. Most of the RFLPs (80%) could be placed with a good level of certainty (LOD>4) on the genetic linkage map. However, because of their dominant nature, only between 37% and 59% of the RAPDs could be placed with such a LOD score. The use of combined data from RFLPs and RAPDs increases the level of information provided by RAPDs and allows the creation of a combined RFLP/RAPD genetic linkage map. Thus, the RAPD technique was found to be a powerful method to provide improved probes coverage on a previously created RFLP map and to locate markers linked to chromosomal regions of interest.
authors.affiliation: Südwestdeutsche Saatzucht, Im Rheinfeld 1-13, 76437, Rastatt, Germany.
authors.forename: V H
authors.initials: VH
authors.lastname: Beaumont
authors.forename: J
authors.initials: J
authors.lastname: Mantet
authors.forename: T R
authors.initials: TR
authors.lastname: Rocheford
authors.forename: J M
authors.initials: JM
authors.lastname: Widholm