title: Formation of terpenoid products in Ginkgo biloba L. cultivated cells.
abstract: Ginkgolides are diterpenes arising from the terpenoid precursor: geranylgeranyl pyrophosphate (GGPP). Incorporation of [1-(14)C] isopentenylpyrophosphate ([1-(14)C]IPP) into GGPP was monitored throughout the cultivation cycle of G. biloba L. cultivated cells. Because incorporation of [1-(14)C]IPP into GGPP had never been monitored in G. biloba, in either the whole plant or cultivated cell system, modifications to existing protocols were necessary. Modifications consisted of extracting the cells with an extraction buffer supplemented with Triton-X-100. Farnesylpyrophosphate (FPP) was the major product formed. The amount of GGPP detected was about one tenth that of FPP.
authors.affiliation: Division of Pharmacognosy, Leiden/Amsterdam Center for Drug Research, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands.
authors.forename: D J
authors.initials: DJ
authors.lastname: Carrier
authors.forename: J
authors.initials: J
authors.lastname: Archambault
authors.forename: R
authors.initials: R
authors.lastname: van der Heijden
authors.forename: R
authors.initials: R
authors.lastname: Verpoorte