title: Development a novel approach of chemiluminescent probe array.
abstract: A new chemiluminescent (CL) probe array assay approach was first developed. The new CL probe array was based on Co3O4-SiO2 mesoporous nanocomposite material, which not only has an excellent catalytic effect on the luminol-H2O2 CL reaction in alkaline medium but also can be used for the immobilization of enzymes. As a model, the novel bifunctional CL probe array has been applied to the high-throughput determination of glucose in human. The linear range of the glucose concentration was 3-90 μM and the detection limit was 0.36 μM. It breaks traditional development view in solid phase supports and provides new insights into the application of mesoporous material.
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, China. Electronic address: zhangzj@snnu.edu.cn.
authors.forename: Zhujun
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Tao
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, China.
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Li
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D001786:Blood Glucose / Q000032:analysis*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D057166:High-Throughput Screening Assays / Q000295:instrumentation; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry*; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000295:instrumentation*; D008165:Luminol / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry / Q000648:ultrastructure; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D004800:Enzymes, Immobilized; D008165:Luminol; D012822:Silicon Dioxide; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase
keywords: Chemiluminescent probe array; Co(3)O(4)–SiO(2) mesoporous nanocomposite material; Glucose; High-throughput; Immobilization