title: The fate of HIV-1 capsid: a biochemical assay for HIV-1 uncoating.
abstract: The uncoating process of HIV-1 is a poorly understood process, so the development of a reliable assay to study uncoating is critical for moving the field forward. Here we describe an uncoating assay that currently represents the state-of-the-art biochemical procedure for monitoring uncoating and core stability during infection. This assay is based on the biochemical separation of soluble capsid protein from particulate capsid cores and provides information about the fate of the capsid during infection.
authors.affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Jeremy
authors.initials: J
authors.lastname: Luban
authors.forename: Felipe
authors.initials: F
authors.lastname: Diaz-Griffero
mesh_terms: D000818:Animals; D002213:Capsid / Q000378:metabolism*; D036022:Capsid Proteins / Q000378:metabolism; D015497:HIV-1 / Q000378:metabolism* / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D021122:Protein Subunits / Q000378:metabolism; D014779:Virus Replication
chemical_list: D036022:Capsid Proteins; D021122:Protein Subunits