title: Purification and properties of a glyphosate-tolerant 5-enolpyruvylshikimate 3-phosphate synthase from the cyanobacterium Anabaena variabilis.
abstract: 5-Enolpyruvylshikimate 3-phosphate (EPSP) synthase (3-phosphoshikimate 1-carboxyvinyltransferase; EC 2.5.1.9) from the glyphosate-tolerant cyanobacterium Anabaena variabilis (ATCC 29413) was purified to homogeneity. The enzyme had a similar relative molecular mass to other EPSP synthases and showed similar kinetic properties except for a greatly elevated K i for the herbicide glyphosate (approximately ten times higher than that of enzymes from other sources). With whole cells, the monoisopropylamine salt of glyphosate was more toxic than the free acid but the effects of the free acid and monoisopropylamine salt on purified EPSP synthase were identical.
authors.affiliation: AFRC Research Group on Cyanobacteria and Department of Biological Sciences, University of Dundee, DD1 4HN, Dundee.
authors.forename: H A
authors.initials: HA
authors.lastname: Powell
authors.forename: N W
authors.initials: NW
authors.lastname: Kerby
authors.forename: P
authors.initials: P
authors.lastname: Rowell
authors.forename: D M
authors.initials: DM
authors.lastname: Mousdale
authors.forename: J R
authors.initials: JR
authors.lastname: Coggins