title: Isolation, identification, and media optimization of high-level cellulase production by Bacillus sp. BCCS A3, in a fermentation system using response surface methodology.
abstract: Cellulases are important glycosyl hydrolase enzymes, which break down cellulose to β-glucose. They have been used widely in biotechnological processing such as bioethanol production. In this work we studied maximizing cellulase production by Bacillus sp. BCCS A3 using response surface methodology (RSM). A good result was attained with these conditions (% w/v): tryptone 0.1, Na₂PO₄ 0.25, (NH₄)₂SO₄ 0.2, MgSO₄ · 7H₂O 0.005, CaCl₂ 0.005, KH₂PO₄ 0.1, NaCl 0.1, sodium carboxymethylcellulose (CMC) 0.75, and pH 9. The cellulase activity in optimized medium was 49.80 U/ml. Moreover, high level of enzyme production was obtained by using fermentor system (50.30 U/ml). Thus, according to the obtained results, this statistical method provided quick identification and integration of key medium details for Bacillus sp. BCCS A3, leading to more cellulase production.
authors.affiliation: a Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences , Shiraz , Iran.
authors.forename: Aboozar
authors.initials: A
authors.lastname: Kazemi
authors.forename: Sara
authors.initials: S
authors.lastname: Rasoul-Amini
authors.forename: Maryam
authors.initials: M
authors.lastname: Shahbazi
authors.forename: Azam
authors.initials: A
authors.lastname: Safari
authors.forename: Younes
authors.initials: Y
authors.lastname: Ghasemi
mesh_terms: D001407:Bacillus / Q000235:genetics / Q000378:metabolism*; D019149:Bioreactors; D002244:Carbon / Q000378:metabolism; D002480:Cellulase / Q000096:biosynthesis* / Q000302:isolation & purification; D003470:Culture Media; D005285:Fermentation; D009584:Nitrogen / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013499:Surface Properties
chemical_list: D003470:Culture Media; D012336:RNA, Ribosomal, 16S; D002244:Carbon; D002480:Cellulase; D009584:Nitrogen