title: Short- and long-term physiological responses of grapevine leaves to UV-B radiation.
abstract: The present study aimed at evaluating the short- and long-term effects of UV-B radiation on leaves of grapevine Vitis vinifera (cv. Tempranillo). Grapevine fruit-bearing cuttings were exposed to two doses of supplemental biologically effective UV-B radiation (UV-BBE) under glasshouse-controlled conditions: 5.98 and 9.66kJm(-2)d(-1). The treatments were applied either for 20d (from mid-veraison to ripeness) or 75d (from fruit set to ripeness). A 0kJm(-2)d(-1) UV-B treatment was included as control. The main effects of UV-B were observed after the short-term exposure (20d) to 9.66kJm(-2)d(-1). Significant decreases in net photosynthesis, stomatal conductance, sub-stomatal CO2 concentration, the actual photosystem II (PSII) efficiency, total soluble proteins and de-epoxidation state of the VAZ cycle were observed, whereas the activities of several antioxidant enzymes increased significantly. UV-B did not markedly affect dark respiration, photorespiration, the maximum potential PSII efficiency (Fv/Fm), non-photochemical quenching (NPQ), as well as the intrinsic PSII efficiency. However, after 75d of exposure to 5.98and 9.66kJm(-2)d(-1) UV-B most photosynthetic and biochemical variables were unaffected and there were no sign of oxidative damage in leaves. The results suggest a high long-term acclimation capacity of grapevine to high UV-B levels, associated with a high accumulation of UV-B absorbing compounds in leaves, whereas plants seemed to be tolerant to moderate doses of UV-B.
authors.affiliation: Grupo de Fisiología del Estrés en Plantas (Dpto. de Biología Ambiental), Unidad Asociada al CSIC, EEAD, Zaragoza e ICVV, Logroño, Facultades de Ciencias y Farmacia, Universidad de Navarra, Irunlarrea 1, 31008 Pamplona, Spain; INRA, Institut des Sciences de la Vigne et du Vin, UMR1287 EGFV, 210 Chemin de Leysotte, 33883 Villenave D'Ornon, France; University of Bordeaux, Institut des Sciences de la Vigne et du Vin, UMR1287 EGFV, 210 Chemin de Leysotte, 33883 Villenave D'Ornon, France.
authors.forename: J
authors.initials: J
authors.lastname: Martínez-Lüscher
authors.forename: F
authors.initials: F
authors.lastname: Morales
authors.forename: S
authors.initials: S
authors.lastname: Delrot
authors.forename: M
authors.initials: M
authors.lastname: Sánchez-Díaz
authors.forename: E
authors.initials: E
authors.lastname: Gomés
authors.forename: J
authors.initials: J
authors.lastname: Aguirreolea
authors.forename: I
authors.initials: I
authors.lastname: Pascual
mesh_terms: D000064:Acclimatization / Q000528:radiation effects*; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002338:Carotenoids / Q000032:analysis / Q000378:metabolism; D019069:Cell Respiration / Q000528:radiation effects; D002734:Chlorophyll / Q000032:analysis / Q000378:metabolism; D015227:Lipid Peroxidation / Q000528:radiation effects; D010084:Oxidation-Reduction / Q000528:radiation effects; D010788:Photosynthesis / Q000528:radiation effects; D045332:Photosystem II Protein Complex / Q000528:radiation effects; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification; D018515:Plant Leaves / Q000502:physiology / Q000528:radiation effects; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism*; D054046:Plant Stomata / Q000502:physiology / Q000528:radiation effects; D018526:Plant Transpiration / Q000528:radiation effects; D013997:Time Factors; D014466:Ultraviolet Rays; D027843:Vitis / Q000502:physiology / Q000528:radiation effects
chemical_list: D000975:Antioxidants; D045332:Photosystem II Protein Complex; D010936:Plant Extracts; D010940:Plant Proteins; D002734:Chlorophyll; D002245:Carbon Dioxide; D002338:Carotenoids
keywords: Antioxidant enzymes; Chlorophyll fluorescence; Photosynthesis; UV-B absorbing compounds; UV-B radiation; Vitis vinifera L.