title: Viral Interferon Regulatory Factor 1 of Kaposi's Sarcoma-Associated Herpesvirus Interacts with a Translocation Liposarcoma Protein-Associated Serine-Arginine Protein.
abstract: OBJECTIVE
  To confirm that Kaposi's sarcoma-associated herpes virus openreading frame K9, viral interferon regulatory factor 1 (vIRF1), interacts with splicing factor, translocation liposarcoma protein-associated serine-arginine protein (TASR), in vivo and to establish whether interactions between vIRF1 and TASRs influence alternative splicing.
  
  
  METHODS
  Association between vIRF1 and TASRs was confirmed with the glutathione S-transferase pull-down assay and coimmunoprecipitation. Further colocalization was shown by immunofluorescence. The in vivo splicing assay was performed to confirm the alterations in the splicing pattern.
  
  
  RESULTS
  vIRF1 interacts with both TASR1 and 2 in vivo. vIRF1 has been shown to colocalize with TASR proteins in 293 T cells. However, an in vivo splicing revealed no alterations in the splicing pattern via interaction.
  
  
  CONCLUSIONS
  The study data suggest that vIRF1 interacts with the TASR protein. However, vIRF1 interactions do not affect TASR-mediated alternative splicing.
authors.affiliation: Department of Life Science, Dongguk University-Seoul, Seoul, Korea.
authors.forename: Sunmi
authors.initials: S
authors.lastname: Kim
authors.forename: Jae Eun
authors.initials: JE
authors.lastname: Jong
authors.forename: Taegun
authors.initials: T
authors.lastname: Seo
keywords: Kaposi’s sarcomaassociated herpesvirus; translocation liposarcoma protein‑associated serine‑arginine protein; viral interferon regulatory factor 1