title: Sendai virus C proteins regulate viral genome and antigenome synthesis to dictate the negative genome polarity.
abstract: The order Mononegavirales comprises a large number of nonsegmented negative-strand RNA viruses (NNSVs). How the genome polarity is determined is a central issue in RNA virus biology. Using a prototypic species, vesicular stomatitis virus (VSV), it has been established that the negative polarity of the viral genome is defined solely by different strengths of the cis-acting replication promoters located at the 3' ends of the genome and antigenome, resulting in the predominance of the genome over the antigenome. This VSV paradigm has long been applied for the Mononegavirales in general without concrete proof. We now found that another prototypic species, Sendai virus (SeV), undergoes a marked shift from the early antigenome-dominant to the late genome-dominant phase during the course of infection. This shift appeared to be governed primarily by the expression of the accessory C protein, because no such shift occurred in a recombinant SeV with the C gene deleted, and antigenomes were dominant throughout infection, generating antigenome-dominant and noninfectious progeny virions. Therefore, we proposed for the first time a trans-regulatory mechanism, the SeV paradigm, to dictate the genome polarity of an NNSV. A series of promoter-swapped SeV recombinants suggested the importance of the primary as well as secondary structures of the promoters in this trans-regulation.
authors.affiliation: Department of Virology, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Irie
authors.forename: Isao
authors.initials: I
authors.lastname: Okamoto
authors.forename: Asuka
authors.initials: A
authors.lastname: Yoshida
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Nagai
authors.forename: Takemasa
authors.initials: T
authors.lastname: Sakaguchi
mesh_terms: D000818:Animals; D002460:Cell Line; D016679:Genome, Viral; D006801:Humans; D029082:Sendai virus / Q000235:genetics / Q000502:physiology*; D014764:Viral Proteins / Q000502:physiology*
chemical_list: D014764:Viral Proteins