title: Kinetics of drug release from a biodegradable local drug delivery system and its effect on Porphyromonas gingivalis isolates: An in vitro study.
abstract: BACKGROUND
  Conventional anti-microbial therapy largely consisted of systemic administration of various drugs effective against periodontal pathogens, but fraught with several problems. Based on the concept of local drug delivery a bioresorbable device made of pure fibrillar collagen has been developed. The aim of this study was to study the release of Tetracycline from this collagen fiber (Type I collagen) impregnated with Tetracycline and its antibacterial activity against Porphyromonas gingivalis.
  
  
  METHODS
  Porphyromonas gingivalis was isolated from plaque samples of chronic periodontitis patients by using a CO2 incubator. DNA isolation was done followed by polymerase chain reaction (PCR) amplification to confirm the presence of bacteria. The release pattern of Tetracycline was assessed for a period of 10 days in water (group I) and Serum inoculated with Porphyromonas gingivalis (group II).
  
  
  RESULTS
  A significant presence of Tetracycline on all days in Group I and group II and the zone of inhibition was also present in both groups with a steady decline from day 1 to day 10.
  
  
  CONCLUSIONS
  Since the results were well within the therapeutic concentration of drug required to inhibit the growth of gram -ve bacteria (Porphyromonas gingivalis), this bioresorbable Tetracycline fiber has the potential for clinical application.
authors.affiliation: Department of Periodontics, Farooqia Dental College and Hospital, Mysore, India.
authors.forename: Ranganathan
authors.initials: R
authors.lastname: Vijayalashmi
authors.forename: Sabitha Manhalore
authors.initials: SM
authors.lastname: Ravindranath
authors.forename: Nadathur Doraiswamy
authors.initials: ND
authors.lastname: Jayakumar
authors.lastname: Padmalatha
authors.forename: Sheeja H
authors.initials: SH
authors.lastname: Vargheese
authors.forename: Kikkeri Laxminarayana
authors.initials: KL
authors.lastname: Kumaraswamy
keywords: Collagen fiber; Porphyromonas gingivalis; polymerase chain reaction; tetracycline