title: [Anti-apoptotic effect of Astragalus Polysaccharide on myeloid cells].
abstract: This study was aimed to assess the effect of Astragalus Polysaccharide (ASPS) on in-vitro hematopoiesis. CFU-GM assays were used to determine the effect of ASPS and thrombopoietin (TPO) on granulocytic-monocyte progenitor cells. The CFU assays were also used to investigate the effect of ASPS on the proliferation of HL-60 cells.HL-60 cells were cultured with serum-free RPMI 1640 medium and treated with or without of different concentrations of ASPS. After 72 h incubation, the number of cells were counted.In addition, the caspase-3 and JC-1 expression was determined by flow cytometry with Annexin V/PI double staining. The results showed that ASPS (100, 200 µg/ml) and TPO (100 ng/ml) significantly promoted CFU-GM formation in vitro. Various concentrations of ASPS and TPO also promoted the colony formation of HL-60 cells, the largest effect of ASPS was observed at a concentration of 100 µg/ml. There were no synergistic effects between TPO and ASPS on cellular proliferation. The results also showed that ASPS significantly protected HL-60 cells from apoptosis in condition of serum-free medium culture, suppressed caspase 3 activation, and reduced the cell apoptosis. It is concluded that ASPS can significantly promote the formation of bone marrow CFU-GM and the proliferation of HL-60 cells, the optimal concentration of ASPS is at 100 µg/ml. In the absence of serum inducing apoptosis, ASPS also significantly reduced the apoptosis of HL-60 cells via suppressing the activation of caspase-3.
authors.affiliation: Institute of Hematology, Jinan University, Guangzhou 510632, Guangdong Province, China; Department of Hematology, Nanfang Hospital, Southern Medical Uniersity, Guangzhou 510515, Guangdong Province, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xiao
authors.forename: Yue
authors.initials: Y
authors.lastname: Xu
authors.forename: Han
authors.initials: H
authors.lastname: He
authors.forename: Qian-Li
authors.initials: QL
authors.lastname: Jiang
authors.forename: Su-Yi
authors.initials: SY
authors.lastname: Li
authors.forename: Hui-Ying
authors.initials: HY
authors.lastname: Shu
authors.forename: En-Yu
authors.initials: EY
authors.lastname: Liang
authors.forename: Zheng-Shan
authors.initials: ZS
authors.lastname: Yi
authors.forename: Jie-Yu
authors.initials: JY
authors.lastname: Ye
authors.forename: Lin-Fang
authors.initials: LF
authors.lastname: Huang
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.forename: Fan-Yi
authors.initials: FY
authors.lastname: Meng
authors.forename: Mo
authors.initials: M
authors.lastname: Yang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D027883:Astragalus Plant; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D018922:HL-60 Cells; D006410:Hematopoiesis / Q000187:drug effects; D006801:Humans; D011134:Polysaccharides / Q000494:pharmacology*; D013926:Thrombopoietin / Q000494:pharmacology
chemical_list: D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D013926:Thrombopoietin; C505407:CASP3 protein, human; D053148:Caspase 3