title: The inhibitory effect of Binens bipinnata L. extract on U14 tumour in mice.
abstract: The objective of this paper was to study the in vitro and in vivo inhibitory effect of Bidens bipinnata L. extract on growth of cervical carcinoma U14 cells. MTT method was used to determine the inhibitory effect of Bidens bipinnata L. extract on U14 tumour cells, and the effects of Bidens bipinnata L. extract on inhibition rate of solid tumour and life prolongation rate of ascites tumour were observed through the establishment of two animal models of mouse cervical carcinoma U14 solid tumour and ascites tumour. In the in vitro MTT assay, the inhibition rate gradually increased with the increase of dose of Bidens bipinnata L. and the extension of time. Its inhibition rate was 70.44% at a concentration of 80µg/L. Solid tumour inhibition rates in the high- and low-dose groups and cisplatin group were 49.13%, 2.26% and 75.72% respectively; life prolongation rates in each ascites tumour group were 63.63%, 34.86% and 87.34% respectively. The Bidens bipinnata L. extract has a certain inhibitory effect on growth of mouse cervical carcinoma U14.
authors.affiliation: The First Affiliated Hospital of Xinxiang Medical College University, Henan Province, China.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Zhu
authors.forename: Rui-Ying
authors.initials: RY
authors.lastname: Qin
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Guo
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Ding
authors.forename: Xin-Lei
authors.initials: XL
authors.lastname: Guan
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Pan
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use*; D031176:Bidens; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D005260:Female; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D002945:Cisplatin
keywords: Bidens bipinnata L. extract; U14; ascites tumour; solid tumour