title: Sequence analysis of the ADRA2A coding region in children affected by attention deficit hyperactivity disorder.
abstract: Attention deficit hyperactivity disorder (ADHD) is a common neurobehavioral pathology characterized by distinct degrees of inattention, hyperactivity and impulsivity. Although ADHD etiology remains elusive, the ADRA2A candidate gene underlies a particular interest, since it participates in the prefrontal cortex regulation of executive function. Three SNPs located on 5' and 3'UTR regions of the gene have been extensively explored but none of them have been definitely validated as a predisposition or a causative sequence variation. In this study, in order to determine whether ADRA2A non-synonymous sequence variants, resulting in biochemical modifications of the protein, are a common cause of the disease we sequenced the complete ADRA2A coding region in a panel of ADHD children of Colombian origin. We identified the c.1138 C>A (p.Arg380Arg) silent substitution. We conclude that ADRA2A non-synonymous sequence variants do not cause ADHD in our sample population. We cannot formerly discard a potential role of this gene during ADHD pathogenesis since only the coding region was analysed. We hope that these results will encourage further researchers to sequence the promoter and coding regions of ADRA2A in large panels of ADHD patients from distinct ethnical origins.
authors.affiliation: Unidad de Genética. Grupo GENIUROS. Escuela de Medicina y Ciencias de la Salud, Universidad del Rosario, Carrera 24 N° 63C-69, Bogotá, Colombia.
authors.forename: Taryn
authors.initials: T
authors.lastname: Castro
authors.forename: Heidi Eliana
authors.initials: HE
authors.lastname: Mateus
authors.forename: Dora Janeth
authors.initials: DJ
authors.lastname: Fonseca
authors.forename: Diego
authors.initials: D
authors.lastname: Forero
authors.forename: Carlos Martín
authors.initials: CM
authors.lastname: Restrepo
authors.forename: Claudia
authors.initials: C
authors.lastname: Talero
authors.forename: Alberto
authors.initials: A
authors.lastname: Vélez
authors.forename: Paul
authors.initials: P
authors.lastname: Laissue
mesh_terms: D001289:Attention Deficit Disorder with Hyperactivity / Q000235:genetics*; D002648:Child; D003105:Colombia; D005260:Female; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D018341:Receptors, Adrenergic, alpha-2 / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: C547768:ADRA2A protein, human; D018341:Receptors, Adrenergic, alpha-2