title: Crystal structure of XoLAP, a leucine aminopeptidase, from Xanthomonas oryzae pv. oryzae.
abstract: Aminopeptidases are metalloproteinases that degrade N-terminal residues from protein and play important roles in cell growth and development by controlling cell homeostasis and protein maturation. We determined the crystal structure of XoLAP, a leucyl aminopeptidase, at 2.6 Å resolution from Xanthomonas oryzae pv. oryzae, causing the destructive rice disease of bacterial blight. It is the first crystal structure of aminopeptidase from phytopathogens as a drug target. XoLAP existed as a hexamer and the monomer structure consisted of an N-terminal cap domain and a C-terminal peptidase domain with two divalent zinc ions. XoLAP structure was compared with BlLAP and EcLAP (EcPepA) structures. Based on the structural comparison, the molecular model of XoLAP in complex with the natural aminopeptidase inhibitor of microginin FR1 was proposed. The model structure will be useful to develop a novel antibacterial drug against Xoo.
authors.affiliation: Department of Biological Sciences, Konkuk University, Seoul, 143-701, Republic of Korea.
authors.forename: Jin-Kwang
authors.initials: JK
authors.lastname: Kim
authors.forename: Sampath
authors.initials: S
authors.lastname: Natarajan
authors.forename: Hanseul
authors.initials: H
authors.lastname: Park
authors.forename: Kim-Hung
authors.initials: KH
authors.lastname: Huynh
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Jeong-Gu
authors.initials: JG
authors.lastname: Kim
authors.forename: Yeh-Jin
authors.initials: YJ
authors.lastname: Ahn
authors.forename: Lin-Woo
authors.initials: LW
authors.lastname: Kang
mesh_terms: D018360:Crystallography, X-Ray; D007931:Leucyl Aminopeptidase / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D014974:Xanthomonas / Q000201:enzymology*; D015032:Zinc / Q000032:analysis
chemical_list: D007931:Leucyl Aminopeptidase; D015032:Zinc