title: Antimicrobial effect of 7-O-butylnaringenin, a novel flavonoid, and various natural flavonoids against Helicobacter pylori strains.
abstract: The antimicrobial effect of a novel flavonoid (7-O-butylnaringenin) on Helicobacter pylori 26695, 51, and SS1 strains and its inhibitory effect on the urease activity of the strains were evaluated and compared with those of several natural flavonoids. First, various flavonoids were screened for antimicrobial activities using the paper disc diffusion method. Hesperetin and naringenin showed the strongest antimicrobial effects among the natural flavonoids tested, and thus hesperetin and naringenin were selected for comparison with 7-O-butylnaringenin. The antimicrobial effect of 7-O-butylnaringenin was greater than that of the hesperetin and naringenin. H. pylori 51 was more sensitive to 7-O-butylnaringenin (2 log reduction of colony forming units, p < 0.05) than the other two strains at 200 μM. 7-O-Butylnaringenin also showed the highest inhibitory effect against urease activity of H. pylori. Morphological changes of H. pylori 26695 treated with these flavonoids indicated that both hesperetin and 7-O-butylnaringenin at 200 μM damaged the cell membranes.
authors.affiliation: Department of Animal Science, Iowa State University, Ames 50011, USA. hdpaik@konkuk.ac.kr.
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Moon
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Kee-Tae
authors.initials: KT
authors.lastname: Kim
authors.forename: Yong-Sun
authors.initials: YS
authors.lastname: Park
authors.forename: Seung-Yeol
authors.initials: SY
authors.lastname: Nah
authors.forename: Dong Uk
authors.initials: DU
authors.lastname: Ahn
authors.forename: Hyun-Dong
authors.initials: HD
authors.lastname: Paik
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D044950:Flavanones / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D006569:Hesperidin / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D014510:Urease / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D044950:Flavanones; D005419:Flavonoids; D006569:Hesperidin; D014510:Urease; C005273:naringenin; C013015:hesperetin