title: Ultrasensitive and fast bottom-up analysis of femtogram amounts of complex proteome digests.
abstract: Femtogram proteomics: An ultrasensitive capillary zone electrophoresis-mass spectrometry system that is based on an improved nanospray interface has been developed. This system is used for the analysis of picogram to femtogram amounts of E. coli digests; for example, over 100 proteins were identified from 16 pg digests by tandem mass spectrometry. AMTs=accurate mass and time tags.
authors.affiliation: Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556 (USA).
authors.forename: Liangliang
authors.initials: L
authors.lastname: Sun
authors.forename: Guijie
authors.initials: G
authors.lastname: Zhu
authors.forename: Yimeng
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Yan
authors.forename: Si
authors.initials: S
authors.lastname: Mou
authors.forename: Norman J
authors.initials: NJ
authors.lastname: Dovichi
mesh_terms: D004586:Electrophoresis; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D020543:Proteome
keywords: electrophoresis; electrospray; mass spectrometry; proteomics; ultrasensitive analysis