title: The first identification of rotavirus B from children and adults with acute diarrhoea in kathmandu, Nepal.
abstract: Rotavirus B (RVB) in the genus Rotavirus of the family Reoviridae is known to be a cause of acute gastroenteritis among children and adults in parts of Asia including China, India, Bangladesh and Myanmar. In a 15-month surveillance programme between March 2007 and May 2008, 3,080 stool specimens were collected from children and adults with acute gastroenteritis in an infectious disease hospital in Kathmandu, Nepal. In 25 (0.8%) specimens RVB was detected, for the first time in Nepal, by the use of polyacrylamide gel electrophoresis followed by confirmation with reverse-transcription PCR and sequencing. The strains detected in this study had very similar electropherotypes, and their VP7 sequences were almost identical and phylogenetically belonged to the Indo-Bangladeshi lineage which was distinct from the Chinese lineage. Thus, this study showed the circulation of RVB strains belonging to the Indo-Bangladeshi lineage in a broader region than previously documented, suggesting that this phylogenetic divide corresponded to the geographic divide created by the Himalayan Mountains. Further studies may be warranted to identify and characterize the RVB strains in northern Vietnam which is adjacent to southern China with a long and less mountainous border.
authors.affiliation: Department of Molecular Microbiology and Immunology, Graduate School of Biomedical Sciences, and the Global Centre of Excellence, Nagasaki University, Nagasaki, Japan.
authors.forename: Md Mahbub
authors.initials: MM
authors.lastname: Alam
authors.forename: Sher B
authors.initials: SB
authors.lastname: Pun
authors.forename: Punita
authors.initials: P
authors.lastname: Gauchan
authors.forename: Michiyo
authors.initials: M
authors.lastname: Yokoo
authors.forename: Yen Hai
authors.initials: YH
authors.lastname: Doan
authors.forename: T N Hoa
authors.initials: TN
authors.lastname: Tran
authors.forename: Toyoko
authors.initials: T
authors.lastname: Nakagomi
authors.forename: Osamu
authors.initials: O
authors.lastname: Nakagomi
authors.forename: Basu D
authors.initials: BD
authors.lastname: Pandey
keywords: Acute diarrhoea; Nepal; Rotavirus B