title: Multivalent interactions of the SUMO-interaction motifs in RING finger protein 4 determine the specificity for chains of the SUMO.
abstract: RNF4 (RING finger protein 4) is a STUbL [SUMO (small ubiquitin-related modifier)-targeted ubiquitin ligase] controlling PML (promyelocytic leukaemia) nuclear bodies, DNA double strand break repair and other nuclear functions. In the present paper, we describe that the sequence and spacing of the SIMs (SUMO-interaction motifs) in RNF4 regulate the avidity-driven recognition of substrate proteins carrying SUMO chains of variable length.
authors.affiliation: †Institute for Genetics, University of Cologne, 50674 Köln, Germany.
authors.forename: Kirstin
authors.initials: K
authors.lastname: Keusekotten
authors.forename: Veronika N
authors.initials: VN
authors.lastname: Bade
authors.forename: Katrin
authors.initials: K
authors.lastname: Meyer-Teschendorf
authors.forename: Annie Miriam
authors.initials: AM
authors.lastname: Sriramachandran
authors.forename: Katrin
authors.initials: K
authors.lastname: Fischer-Schrader
authors.forename: Anke
authors.initials: A
authors.lastname: Krause
authors.forename: Christiane
authors.initials: C
authors.lastname: Horst
authors.forename: Günter
authors.initials: G
authors.lastname: Schwarz
authors.forename: Kay
authors.initials: K
authors.lastname: Hofmann
authors.forename: R Jürgen
authors.initials: RJ
authors.lastname: Dohmen
authors.forename: Gerrit J K
authors.initials: GJ
authors.lastname: Praefcke
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D054730:Protein Interaction Domains and Motifs / Q000502:physiology; D025842:SUMO-1 Protein / Q000378:metabolism*; D012441:Saccharomyces cerevisiae; D013379:Substrate Specificity; D058207:Sumoylation / Q000502:physiology*; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D009687:Nuclear Proteins; C114070:RNF4 protein, human; D025842:SUMO-1 Protein; D014157:Transcription Factors