title: Effects of plant growth substances on rooting of Hedychium spicatum under different temperature regimes.
abstract: Present study was carried out to develop a simple and efficient vegetative propagation protocol by applying various treatments to rhizome cuttings with different test solutions of auxins and phenolic compound. These were alpha-naphthalene acetic acid (NAA), Indole-3-butyric acid (IBA), Indole Acetic Acid (IAA), phloroglucinol and coumarin. The concentrations for each treatment were 10.0, 50.0 and 100.0 microM. After treatments the rhizome cuttings were planted in polybags containing forest soil and kept under different temperature regimes i.e., inside polyhose (at 20-25 degrees C), inside mist chamber (at 15-20 degrees C) and under nethouse (nursery condition, at 14-18 degrees C). The maximum rooting percentage (74.06%) was achieved at 20-25 degrees C (inside polyhouse) by applying 50.0 microM IBA. Inside poly house condition, the various developmental parameters showed better responses compare to other conditions. On the basis of present study emphasizes that the temperature play a crucial role in rooting and further growth of the plants in this species. By using this simple and significant conventional method of propagation we could be propagate this vulnerable medicinal and aromatic species at large scale for commercial purpose.
authors.affiliation: Plant Tissue Culture Laboratory, Department of Botany, D.S.B. Campus, Kumaun University, Nainital, Uttarakhand 263002, India.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Giri
authors.forename: Sushma
authors.initials: S
authors.lastname: Tamta
mesh_terms: D063245:Plant Development / Q000187:drug effects / Q000502:physiology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D027343:Rhizome / Q000187:drug effects / Q000254:growth & development; D013696:Temperature; D030521:Zingiberaceae / Q000187:drug effects* / Q000254:growth & development*
chemical_list: D010937:Plant Growth Regulators