title: Enantioselective extraction of (+)-(S)-citalopram and its main metabolites using a tailor-made stir bar chiral imprinted polymer for their LC-ESI-MS/MS quantitation in urine samples.
abstract: This paper reports the application of a chiral imprinted polymer (CIP)-coated stir bar for the selective extraction of (+)-(S)-citalopram (SCIT) and its main metabolites, (+)-(S)-desmethylcitalopram (SDCIT) and (+)-(S)-didesmethylcitalopram (SDDCIT), from urine samples. The developed device has been demonstrated to be capable of selectively extracting the three target analytes from urine samples without saturating the imprinted sites. A CIP-coated stir bar sorptive extraction procedure (CIP-SBSE) is proposed for the isolation of SCIT, SDCIT and SDDCIT followed by their subsequent analysis using liquid chromatography ion trap mass spectrometry (LC-ITMS). Deuterated SCIT-d6 was used as an internal standard. The method was validated using a standard procedure, which revealed that a quantification of 5 ng mL(-1) was obtained in urine samples and that the accuracy and precision were within the established values while no matrix effect was observed.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, University of the Basque Country, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain.
authors.forename: Nora
authors.initials: N
authors.lastname: Unceta
authors.forename: Alberto
authors.initials: A
authors.lastname: Gómez-Caballero
authors.forename: Deiene
authors.initials: D
authors.lastname: García
authors.forename: Goretti
authors.initials: G
authors.lastname: Díaz
authors.forename: Antonio
authors.initials: A
authors.lastname: Guerreiro
authors.forename: Sergey
authors.initials: S
authors.lastname: Piletsky
authors.forename: M Aránzazu
authors.initials: MA
authors.lastname: Goicolea
authors.forename: Ramón J
authors.initials: RJ
authors.lastname: Barrio
mesh_terms: D000327:Adsorption; D018687:Antidepressive Agents, Second-Generation / Q000652:urine*; D002853:Chromatography, Liquid; D015283:Citalopram / Q000031:analogs & derivatives* / Q000652:urine*; D006801:Humans; D054802:Molecular Imprinting / Q000379:methods; D011108:Polymers / Q000737:chemistry*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods / Q000592:standards*; D021241:Spectrometry, Mass, Electrospray Ionization; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry
chemical_list: D018687:Antidepressive Agents, Second-Generation; D011108:Polymers; D015283:Citalopram; C046304:desmethylcitalopram; C064735:didesmethylcitalopram
keywords: LC-ESI-ITMS; S-citalopram; S-desmethylcitalopram; S-didesmethylcitalopram: chiral imprinted polymer (CIP)-coated stir bar; Urine