title: Evaluation of a method using high performance liquid chromatography with ultraviolet detection for the determination of statins in macromycetes of the genus Pleurotus cultivated by fermentation processes.
abstract: The applicability of high-performance liquid chromatography with ultraviolet light (HPLC-UV) for the determination of the presence of statins in macromycetes of the genus Pleurotus was analyzed. The fungi were obtained by liquid-state fermentation (LSF) using unconventional sources of carbon as substrates and solid-state fermentation (SSF) employing agro industrial wastes. Five statins were used as standards: lovastatin and simvastatin in the lactone form (LOVL and SIML), their corresponding hydro-acidic forms (LOVH and SIMH) and pravastatin (PRA). The following measures were evaluated: the linearity, accuracy and precision, detection limit (DL) and quantification limit (QL). The results demonstrated HPLC-UV to be an effective tool for detecting the presence of statins in extracts of LSF and SSF products. Likewise, it was hypothesized that the strains that were used for the study do not produce statins. This finding highlights the importance of continuing to evaluate other strains of the same genus by using techniques such as HPLC to first separate sufficient quantities of the compounds that were detected using the standard technique but that did not match the retention time (tR) of any of the standards used.
authors.affiliation: Department of Chemistry, National University of Colombia, AA 14490, Bogota, Colombia. Electronic address: cchegwina@unal.edu.co.
authors.forename: Carolina
authors.initials: C
authors.lastname: Chegwin-Angarita
authors.forename: Ivonne
authors.initials: I
authors.lastname: Jeannette Nieto-Ramirez
authors.forename: Gonzalo J
authors.initials: GJ
authors.lastname: Diaz
authors.forename: Janeth Rocío
authors.initials: JR
authors.lastname: Rojas L
authors.forename: Liuda
authors.initials: L
authors.lastname: Sepúlveda
authors.forename: Lucia
authors.initials: L
authors.lastname: Atehortúa
mesh_terms: D000383:Agriculture; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D004355:Drug Stability; D005285:Fermentation; D057230:Limit of Detection; D008148:Lovastatin / Q000096:biosynthesis / Q000302:isolation & purification*; D020076:Pleurotus / Q000378:metabolism*; D017035:Pravastatin / Q000096:biosynthesis / Q000302:isolation & purification*; D019821:Simvastatin / Q000302:isolation & purification* / Q000378:metabolism; D014866:Waste Products
chemical_list: D014866:Waste Products; D008148:Lovastatin; D019821:Simvastatin; D017035:Pravastatin
keywords: CS; DAD; DL; EtOAc; HCl; HPLC; HPLC-UV.; High performance liquid chromatography; KOH; LOVH; LSF; Liquid-state fermentation; NaOH; PRA; Pleurotus; QL; RYD; RYN; RYS; SIMH; SIML; SSF; Solid-state fermentation; Statins; UV; carbon source; chlorhydric acid; diode array detector; ethyl acetate; fermented red rice “Red Yeast Rice(®)” Nature´s Plus; fermented red rice “Traditional Red Yeast®” Swanson Premium Brand; fermented red rice, “Traditional Red Yeast(®)” Doctor’s A-Z; limit of detection; limit of quantitation; liquid state fermentations; lovastatin in hydroxy acid form LOVL, lovastatin in lactone form; potassium hydroxide; pravastatin; retention time; simvastatin in hydroxy acid form; simvastatin in lactone form; sodium hydroxide; solid state fermentations; t(R); ultraviolet