title: An improve method for somatic embryogenesis of Schisandra chinensis (Turcz.) Baillon.
abstract: A somatic embryogenesis protocol for plant regeneration of Schisandra chinensis (Turcz.) Baillon was established. Increased callus induction was obtained from mature zygotic embryos on Murashige and Skoog (MS) medium supplemented with 1.0 mg L(-1) N-phenyl-1,2,3-thidiazol-5-yl-urea (TDZ) or 2.0 mg L(-1) 2,4-dichlorophenoxyacetic acid (2,4-D). Addition of Zeatin (Zt) promoted the formation of embryogenic calli. To induce somatic embryogenesis, 2,4-D, TDZ and Zt were incorporated in the medium alone or in combination. Development of the maximum number of somatic embryos (81 globular, 37 heart, 52 torpedo and 37 cotyledon-stage) and germination of the highest number of embryos (50%) was observed on 1/2 MS medium supplemented with 1.0 mg L(-1) TDZ and 0.2 mg L(-1) Zt. Further development of somatic embryos into plantlets was completed in 1/2 MS medium free of plant growth regulators.
authors.affiliation: College of Horticulture, Shenyang Agricultural University, Dongling Road 120, Shenyang 110866, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Sun
authors.forename: Wen-Xiang
authors.initials: WX
authors.lastname: Lang
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Li
authors.forename: Xiu-Wu
authors.initials: XW
authors.lastname: Guo
authors.forename: Mei-Lan
authors.initials: ML
authors.lastname: Lian
authors.forename: Zhong-Yun
authors.initials: ZY
authors.lastname: Piao
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry; D018525:Germination; D010671:Phenylurea Compounds / Q000737:chemistry; D010937:Plant Growth Regulators / Q000737:chemistry; D018520:Plant Shoots / Q000254:growth & development; D058439:Plant Somatic Embryogenesis Techniques / Q000379:methods*; D032141:Schisandra / Q000254:growth & development*; D012639:Seeds / Q000254:growth & development; D013830:Thiadiazoles / Q000737:chemistry; D015026:Zeatin / Q000737:chemistry
chemical_list: D003470:Culture Media; D010671:Phenylurea Compounds; D010937:Plant Growth Regulators; D013830:Thiadiazoles; C016785:thidiazuron; D015084:2,4-Dichlorophenoxyacetic Acid; D015026:Zeatin