title: Effect of C/N ratio and media optimization through response surface methodology on simultaneous productions of intra- and extracellular inulinase and invertase from Aspergillus niger ATCC 20611.
abstract: The study is to identify the extraction of intracellular inulinase (exo- and endoinulinase) and invertase as well as optimization medium composition for maximum productions of intra- and extracellular enzymes from Aspergillus niger ATCC 20611. From two different methods for extraction of intracellular enzymes, ultrasonic method was found more effective. Response surface methodology (RSM) with a five-variable and three-level central composite design (CCD) was employed to optimize the medium composition. The effect of five main reaction parameters including sucrose, yeast extract, NaNO₃, Zn⁺², and Triton X-100 on the production of enzymes was analyzed. A modified quadratic model was fitted to the data with a coefficient of determination (R²) more than 0.90 for all responses. The intra-extracellular inulinase and invertase productions increased in the range from 16 to 8.4 times in the optimized medium (10% (w/v) sucrose, 2.5% (w/v) yeast extract, 2% (w/v) NaNO₃, 1.5 mM (v/v) Zn⁺², and 1% (v/v) Triton X-100) by RSM and from around 1.2 to 1.3 times greater than in the medium optimized by one-factor-at-a-time, respectively. The results of bioprocesses optimization can be useful in the scale-up fermentation and food industry.
authors.affiliation: Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Mojdeh
authors.initials: M
authors.lastname: Dinarvand
authors.forename: Malahat
authors.initials: M
authors.lastname: Rezaee
authors.forename: Malihe
authors.initials: M
authors.lastname: Masomian
authors.forename: Seyed Davoud
authors.initials: SD
authors.lastname: Jazayeri
authors.forename: Mohsen
authors.initials: M
authors.lastname: Zareian
authors.forename: Sahar
authors.initials: S
authors.lastname: Abbasi
authors.forename: Arbakariya B
authors.initials: AB
authors.lastname: Ariff
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D019149:Bioreactors; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D019649:Food Industry; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000302:isolation & purification*; D009566:Nitrates / Q000737:chemistry; D013395:Sucrose / Q000737:chemistry
chemical_list: D003470:Culture Media; D009566:Nitrates; D013395:Sucrose; C031618:sodium nitrate; D006026:Glycoside Hydrolases; C023086:inulinase