title: Analysis of recombination rate in female and male gametogenesis in pearl millet (Pennisetum glaucum) using RFLP markers.
abstract: Sex as a factor affecting recovered recombination in plant gametes was investigated in pearl millet, Pennisetum glaucum, by using reciprocal three-way crosses [(AxB)xCvCx(A x B)]. The two populations were mapped at 42 loci pre-selected to cover the majority of the genome. No differences in recombination distances were observed at the whole-genome level and only a few individual linkage intervals were found to differ, all in favour of increased recombination through the male. Distorted segregations found in the three-way crosses provide evidence of post-gametic selection for particular gene(s) or chromosome regions. The significance of these results for the design of pearl millet breeding programmes and inheritance experiments, as well as for other experimental strategies, is discussed.
authors.affiliation: Cambridge Laboratory, NR4 7UJ, Colney, Norwich, UK.
authors.forename: C S
authors.initials: CS
authors.lastname: Busso
authors.forename: C J
authors.initials: CJ
authors.lastname: Liu
authors.forename: C T
authors.initials: CT
authors.lastname: Hash
authors.forename: J R
authors.initials: JR
authors.lastname: Witcombe
authors.forename: K M
authors.initials: KM
authors.lastname: Devos
authors.forename: J M
authors.initials: JM
authors.lastname: de Wet
authors.forename: M D
authors.initials: MD
authors.lastname: Gale