title: Enabling autologous human liver regeneration with differentiated adipocyte stem cells.
abstract: We developed a novel method for differentiating adipocyte-derived stem cells (ASCs) into hepatocyte-like cells (iHeps). ASCs are cultured as spherical cellular aggregates and are then induced by culture in chemically defined media for a short time period to differentiate into spherical culture iHeps (SCi-Heps). SCi-Heps have many of the in vitro functional properties of mature hepatocytes, and they can stably reconstitute functioning human liver in vivo in a murine model system. Implantation studies demonstrate that SCi-Heps have a very low malignant potential. All human liver regenerative procedures, including ultrasound-guided direct liver implantation, are scalable and appropriate for human clinical use. These methods can be used to achieve the major promise of regenerative medicine. It may now be possible to regenerate human liver using autologous stem cells obtained from a readily accessible tissue.
authors.affiliation: Department of Anesthesia, Stanford University School of Medicine, Stanford, CA, USA.
authors.forename: Dan
authors.initials: D
authors.lastname: Xu
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Nishimura
authors.forename: Ming
authors.initials: M
authors.lastname: Zheng
authors.forename: Manhong
authors.initials: M
authors.lastname: Wu
authors.forename: Hua
authors.initials: H
authors.lastname: Su
authors.forename: Noboru
authors.initials: N
authors.lastname: Sato
authors.forename: Gordon
authors.initials: G
authors.lastname: Lee
authors.forename: Sara
authors.initials: S
authors.lastname: Michie
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Glenn
authors.forename: Gary
authors.initials: G
authors.lastname: Peltz
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D063646:Carcinogenesis / Q000473:pathology; D002454:Cell Differentiation; D005786:Gene Expression Regulation; D006801:Humans; D016867:Immunocompromised Host; D008115:Liver Regeneration; D008297:Male; D008822:Mice, Transgenic; D013234:Stem Cells / Q000166:cytology*; D014182:Transplantation, Autologous