title: Count your eggs before they invade: identifying and quantifying egg clutches of two invasive apple snail species (Pomacea).
abstract: Winning the war against invasive species requires early detection of invasions. Compared to terrestrial invaders, aquatic species often thrive undetected under water and do not garner notice until too late for early action. However, fortunately for managers, apple snails (Family Ampullariidae, Genus Pomacea) provide their own conspicuous sign of invasion in the form of vibrantly colored egg clutches. Managers can potentially use egg clutches laid in the riparian zone as a means of early detection and species identification. To facilitate such efforts, we quantified differences in characteristics (length, width, depth, mass, egg number) of field-laid clutches for the two most common invasive species of apple snail, P. canaliculata and P. maculata, in native and non-native populations. Pomacea canaliculata native and non-native populations differed noticeably only in width. Native P. maculata clutches possessed significantly greater width, mass and eggs numbers compared with native P. canaliculata. Non-native P. maculata clutches significantly exceeded all other populations in all measured characteristics. Consequently, these traits may successfully distinguish between species. Fecundity data also allowed us to develop models that accurately estimated the number of eggs per clutch for each species based on clutch dimensions. We tested one, two and three dimensional models of clutches, including rendering a clutch as either a complete ellipsoid or an ellipsoid intersected by a cylinder to represent the oviposition site. Model comparisons found the product of length and depth, with a different function for each population, best predicted egg number for both species. Comparisons of egg number to clutch volume and mass implied non-native P. canaliculata may be food limited, while non-native P. maculata appeared to produce such enormous clutches by having access to greater nutrients than the native population. With these new tools, researchers and managers can quickly identify, quantify and begin eradication of new non-native apple snail populations.
authors.affiliation: Department of Ecology and Evolution, University of Chicago, Chicago, Illinois, United States of America.
authors.forename: Colin H
authors.initials: CH
authors.lastname: Kyle
authors.forename: Allyson L
authors.initials: AL
authors.lastname: Plantz
authors.forename: Therese
authors.initials: T
authors.lastname: Shelton
authors.forename: Romi L
authors.initials: RL
authors.lastname: Burks
mesh_terms: D000818:Animals; D005260:Female; D058865:Introduced Species; D027845:Malus / Q000469:parasitology; D010058:Oviposition / Q000502:physiology; D012908:Snails / Q000472:pathogenicity / Q000502:physiology*