title: X-ray solution structure of the native neuronal porosome-synaptic vesicle complex: Implication in neurotransmitter release.
abstract: Nanoportals at the cell plasma membrane called porosomes, mediate secretion from cells. In neurons porosomes are 15 nm cup-shaped lipoprotein structure composed of nearly 40 proteins. The size and complexity of the porosome has precluded determination of its atomic structure. Here we report at nanometer resolution the native 3D structure of the neuronal porosome-synaptic vesicle complex within isolated nerve terminals using small-angle X-ray solution scattering. In addition to furthering our understanding of the porosome structure, results from the study suggests the molecular mechanism involved in neurotransmitter release at the nerve terminal.
authors.affiliation: Wayne State University School of Medicine, Department of Biochemistry and Molecular Biology, Detroit, MI, USA.
authors.forename: Ladislau C
authors.initials: LC
authors.lastname: Kovari
authors.forename: Joseph S
authors.initials: JS
authors.lastname: Brunzelle
authors.forename: Kenneth T
authors.initials: KT
authors.lastname: Lewis
authors.forename: Won Jin
authors.initials: WJ
authors.lastname: Cho
authors.forename: Jin-Sook
authors.initials: JS
authors.lastname: Lee
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Taatjes
authors.forename: Bhanu P
authors.initials: BP
authors.lastname: Jena
mesh_terms: D000818:Animals; D001921:Brain / Q000648:ultrastructure; D002462:Cell Membrane / Q000648:ultrastructure; D008854:Microscopy, Electron; D009474:Neurons / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley; D009435:Synaptic Transmission; D013572:Synaptic Vesicles / Q000648:ultrastructure*; D013574:Synaptosomes / Q000648:ultrastructure*
keywords: Electron microscopy; Neuronal porosome complex; Photon correlation spectroscopy; X-ray solution scattering