title: Organogenesis in vitro of Nothofagus alpina (P. et E.) Oerst, Fagaceae.
abstract: In vitro shoot and root regeneration of 2-year-old Nothofagus alpina plants was achieved from several types of expiants cultured in vitro on a modified Woody Plant Medium formulation. Multiple shoot formation was obtained from leaf expiants using 0.45-2.27 μM thidiazuron and 0.0049-0.098 μM indolebutyric acid. Excised axillary buds formed shoots and roots in the presence of 0.0049 μM benzyladenine and 2.46 μM indolebutyric acid, or in the absence of plant growth regulators. Nodal sections rooted when 2.46 μM indolebutyric acid at was supplied in the medium. Subcultured shoots originating from nodal sections showed a high regeneration rate through multiple shoot and root formation.
authors.affiliation: Departamento de Ecología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Casilla, 114-D, Santiago, Chile.
authors.forename: M
authors.initials: M
authors.lastname: Jordan
authors.forename: J
authors.initials: J
authors.lastname: Velozo
authors.forename: A M
authors.initials: AM
authors.lastname: Sabja