title: Proteins pinpoint double strand breaks.
abstract: Combining green fluorescent protein with a protein that only binds to double strand breaks in DNA allows these breaks-which are an important form of DNA damage-to be detected with high efficiency in living bacteria.
authors.affiliation: is at the Department of Biochemistry , University of Wisconsin-Madison , Madison , United States cox@biochem.wisc.edu.
authors.forename: Michael M
authors.initials: MM
authors.lastname: Cox
mesh_terms: D000818:Animals; D002876:Chromosomes, Bacterial / Q000378:metabolism*; D053903:DNA Breaks, Double-Stranded; D004268:DNA-Binding Proteins / Q000235:genetics*; D006801:Humans; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C065410:gam protein, Coliphage
keywords: DNA double-strand breaks; E. coli; GFP; Human; Mouse; endogenous DNA damage; fluorescent-protein fusions; spontaneous DNA breaks; synthetic biology