title: Engineered P450pyr monooxygenase for asymmetric epoxidation of alkenes with unique and high enantioselectivity.
abstract: A triple mutant of P450pyr monooxygenase (P450pyrTM) catalysed the epoxidation of several para-substituted styrenes as the first enzyme showing high (R)-enantioselectivity and high conversion, demonstrated a broad substrate range, and showed high enantioselectivity for the epoxidation of an unconjugated 1,1-disubstituted alkene, 2-methyl-3-phenyl-1-propene, and a cyclic alkene, N-phenoxycarbonyl-1,2,5,6-tetrahydropyridine, respectively.
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576, Singapore. chelz@nus.edu.sg.
authors.forename: Aitao
authors.initials: A
authors.lastname: Li
authors.forename: Ji
authors.initials: J
authors.lastname: Liu
authors.forename: Son Q
authors.initials: SQ
authors.lastname: Pham
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
mesh_terms: D000475:Alkenes / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry*; D004852:Epoxy Compounds / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology; D013237:Stereoisomerism; D013343:Styrenes / Q000737:chemistry*
chemical_list: D000475:Alkenes; D001426:Bacterial Proteins; D004852:Epoxy Compounds; D013343:Styrenes; D003577:Cytochrome P-450 Enzyme System