title: Molecular detection of Salmonella species in bovine fecal samples.
abstract: A study was performed to assess the validity of the BAX automated polymerase chain reaction system (DuPont Nutrition & Health, Wilmington, Delaware) to detect the shedding of Salmonella species in bovine fecal samples. A total of 133 bovine fecal samples that were submitted to the Cornell University Animal Health Diagnostic Laboratory for Salmonella culture were also tested in the BAX system with a modified version of the manufacturer's enrichment protocol. Using culture as the gold standard test, the BAX system was found to have a sensitivity of 85.7% and a specificity of 90.5%. There was excellent agreement (κ = 0.71, standard error = 0.072) and no significant differences between the 2 methods (McNemar χ(2) = 0.180).
authors.affiliation: 1Hussni O. Mohammed, Department of Population and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, S1 070 Schurman Hall, Ithaca, NY 14853. hom1@cornell.edu.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Bordonaro
authors.forename: Patrick L
authors.initials: PL
authors.lastname: McDonough
authors.forename: Yung-fu
authors.initials: YF
authors.lastname: Chang
authors.forename: Hussni O
authors.initials: HO
authors.lastname: Mohammed
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000382:microbiology*; D016009:Chi-Square Distribution; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011237:Predictive Value of Tests; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification*; D012481:Salmonella Infections, Animal / Q000175:diagnosis / Q000382:microbiology*; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial
keywords: Cattle; Salmonella; feces; polymerase chain reaction; validation