title: Proteomics analysis of UV-irradiated Lonicera japonica Thunb. with bioactive metabolites enhancement.
abstract: A previous study showed that the contents of caffeoylquinic acids and iridoids, the major bioactive components in the postharvest Lonicera japonica Thunb., were induced by enhanced ultraviolet (UV)-A or UV-B irradiation. To clarify the UV-responsive key enzymes in the bioactive metabolites biosynthetic pathway and the related plant defense mechanism in L. japonica, 2DE in combination with MALDI-TOF/TOF MS was employed. Seventy-five out of 196 differential proteins were positively identified. Based on the functions, these proteins were grouped into nine categories, covering a wide range of molecular processes including the secondary metabolites (caffeoylquinic acids and iridoids) biosynthetic-related proteins, photosynthesis, carbohydrate and energy metabolism, stress, DNA, transport-related proteins, lipid metabolism, amino acid metabolism, cell wall. Of note is the increasing expression of 1-deoxy-d-xylulose 5-phosphate reductoisomerase and 5-enol-pyruvylshikimate-phosphate synthase, which was crucial to supply more precursor for the secondary metabolites including caffeoylquinic acids and iridoids. Thus, this study provides both the clues at the protein level for the increase of the two bioactive components upon UV irradiation and the profile of UV-responsive proteins in L. japonica.
authors.affiliation: Key Laboratory for Biomedical Engineering, Zhejiang University, Ministry of Education, Hangzhou, Zhejiang, P. R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Ximin
authors.initials: X
authors.lastname: Li
authors.forename: Wen
authors.initials: W
authors.lastname: Zheng
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Fu
authors.forename: Wenting
authors.initials: W
authors.lastname: Li
authors.forename: Luyu
authors.initials: L
authors.lastname: Ma
authors.forename: Ke
authors.initials: K
authors.lastname: Li
authors.forename: Lianli
authors.initials: L
authors.lastname: Sun
authors.forename: Jingkui
authors.initials: J
authors.lastname: Tian
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D035264:Flowers / Q000378:metabolism* / Q000528:radiation effects; D031239:Lonicera / Q000378:metabolism* / Q000528:radiation effects; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D060066:Protein Interaction Maps; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological; D014466:Ultraviolet Rays
chemical_list: D010940:Plant Proteins; D020543:Proteome; D010650:Phenylalanine Ammonia-Lyase
keywords: Biosynthetic pathway; Lonicera japonica Thunb; Plant proteomics; Secondary metabolites; UV irradiation