title: BdorCSP2 is important for antifeed and oviposition-deterring activities induced by Rhodojaponin-III against Bactrocera dorsalis.
abstract: Rhodojaponin-III is a nonvolatile botanical grayanoid diterpene compound, which has antifeedant and oviposition deterrence effects against many kinds of insects. However, the molecular mechanism of the chemoreception process remains unknown. In this study, the important role of BdorCSP2 in the recognition of Rhodojaponin-III was identified. The full length cDNA encoding BdorCSP2 was cloned from legs of Bactrocera dorsalis. The results of expression pattern revealed that BdorCSP2 was abundantly expressed in the legs of adult B. dorsalis. Moreover, the expression of BdorCSP2 could be up-regulated by Rhodojaponin-III. In order to gain comprehensive understanding of the recognition process, the binding affinity between BdorCSP2 and Rhodojaponin-III was measured by fluorescence binding assay. Silencing the expression of BdorCSP2 through the ingestion of dsRNA could weaken the effect of oviposition deterrence and antifeedant of Rhodojaponin-III. These results suggested that BdorCSP2 of B. dorsalis could be involved in chemoreception of Rhodojaponin-III and played a critical role in antifeedant and oviposition behaviors induced by Rhodojaponin-III.
authors.affiliation: Laboratory of Insect Toxicology, Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yi
authors.forename: Haiming
authors.initials: H
authors.lastname: Zhao
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Dong
authors.forename: Peidan
authors.initials: P
authors.lastname: Wang
authors.forename: Meiying
authors.initials: M
authors.lastname: Hu
authors.forename: Guohua
authors.initials: G
authors.lastname: Zhong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D057506:Arthropod Antennae / Q000187:drug effects / Q000502:physiology; D001667:Binding, Competitive / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D005247:Feeding Behavior / Q000187:drug effects*; D005453:Fluorescence; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D019476:Insect Proteins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D010058:Oviposition / Q000187:drug effects*; D010802:Phylogeny; D034622:RNA Interference / Q000187:drug effects; D011817:Rabbits; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D033621:Tephritidae / Q000187:drug effects* / Q000502:physiology*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D004224:Diterpenes; D019476:Insect Proteins; C069461:rhodojaponin III