title: Differential expression of αB-crystallin causes maturation-dependent susceptibility of oligodendrocytes to oxidative stress.
abstract: Oligodendrocyte precursor cells (OPCs) are most susceptible to oxidative stress in the brain. However, the cause of differences in susceptibility to oxidative stress between OPCs and mature oligodendrocytes (mOLs) remains unclear. Recently, we identified in vivo that αB-crystallin (aBC) is expressed in mOLs but not in OPCs. Therefore, we examined in the present study whether aBC expression could affect cell survival under oxidative stress induced by hydrogen peroxide using primary cultures of OPCs and mOLs from neonatal rat brains. Expression of aBC was greater in mOLs than in OPCs, and the survival rate of mOLs was significantly higher than that of OPCs under oxidative stress. Suppression of aBC by siRNA transfection resulted in a decrease in the survival rate of mOLs under oxidative stress. These data suggest that higher susceptibility of OPCs than mOLs to oxidative stress is due, at least in part, to low levels of aBC expression.
authors.affiliation: Departments of Anatomy, Chungbuk National University School of Medicine, Cheongju 361-763, Korea seojh@chungbuk.ac.kr.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Lee
authors.forename: Hyun Joon
authors.initials: HJ
authors.lastname: Sohn
authors.forename: Si Wook
authors.initials: SW
authors.lastname: Kim
authors.forename: Chan Hyung
authors.initials: CH
authors.lastname: Kim
authors.forename: Hee Yul
authors.initials: HY
authors.lastname: Ahn
authors.forename: Dong Woon
authors.initials: DW
authors.lastname: Kim
authors.forename: Sa Sun
authors.initials: SS
authors.lastname: Cho
authors.forename: Je Hoon
authors.initials: JH
authors.lastname: Seo
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D003459:Crystallins / Q000235:genetics / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009836:Oligodendroglia / Q000378:metabolism*; D018384:Oxidative Stress / Q000235:genetics / Q000502:physiology; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003459:Crystallins; D034741:RNA, Small Interfering; D006861:Hydrogen Peroxide