title: Development of a multiresidue method for the determination of endocrine disrupters in fish fillet using gas chromatography-triple quadrupole tandem mass spectrometry.
abstract: Endocrine Disrupter Compounds (EDCs) are responsible for alterations in the endocrine system functions. Aquatic organisms are able to accumulate EDCs residues, being the major source of contamination for top predators and human consumers. This study aimed to develop and validate a method for the determination of 40 EDCs in fish fillet using modified QuEChERS and Gas Chromatography coupled with Mass Spectrometry in tandem (GC-MS/MS). A factorial design was used to optimize the extraction procedure. Method validation presented recoveries from 70.1% to 120.0% with RSD<20% and method limit of detection ranged from 0.3 to 7.5 µg kg(-1), showing good accuracy and precision. This method was successfully applied to the analysis of fish fillet from different species and residues of bisphenol A, chlorpyrifos and bifenthrin were detected. The proposed method proved to be effective for the determination of EDCs in fish fillet at very low concentration levels.
authors.affiliation: Laboratory of Pesticide Residue Analysis (LARP), Chemistry Department, Federal University of Santa Maria, 97105-900 Santa Maria-RS, Brazil.
authors.forename: Juliana S
authors.initials: JS
authors.lastname: Munaretto
authors.forename: Giovana
authors.initials: G
authors.lastname: Ferronato
authors.forename: Lucila C
authors.initials: LC
authors.lastname: Ribeiro
authors.forename: Manoel L
authors.initials: ML
authors.lastname: Martins
authors.forename: Martha B
authors.initials: MB
authors.lastname: Adaime
authors.forename: Renato
authors.initials: R
authors.lastname: Zanella
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000302:isolation & purification*; D004390:Chlorpyrifos / Q000302:isolation & purification*; D052244:Endocrine Disruptors / Q000302:isolation & purification*; D005163:Factor Analysis, Statistical; D005396:Fish Products / Q000032:analysis*; D005399:Fishes; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D057230:Limit of Detection; D010636:Phenols / Q000302:isolation & purification*; D011722:Pyrethrins / Q000302:isolation & purification*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004390:Chlorpyrifos; D052244:Endocrine Disruptors; D010636:Phenols; D011722:Pyrethrins; D014874:Water Pollutants, Chemical; C099952:bifenthrin; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Endocrine disrupters; Fish fillet; GC–MS/MS; QuEChERS