title: [Anti-inflammation of simvastatin by polarization of murine macrophages from M1 phenotype to M2 phenotype].
abstract: OBJECTIVE
  To explore the effects of statin on pro-inflammatory macrophage phenotype in a murine M1 macrophage model.
  
  
  METHODS
  Macrophages isolated from murine bone barrow were stimulated with interferon-gamma (IFN-γ) and lipopolysaccharide (LPS) to establish a M1 macrophage model. And 1.0, 2.5, 5.0 µmol/L of simvastatin were added to M1 macrophages for a 9-hour culture. Cell surface markers CD16/23 and CD206 were detected by fluorescence activated cell sorter (FACS) and interleukin-10 (IL-10) and IL-12 by ELISA.
  
  
  RESULTS
  The CD16/32 expression was 86.39% ± 2.24% and IL-12 secretion (1562 ± 217) pg/ml in IFN-γ and LPS-stimulated macrophages. After a 9-hour incubation with 1.0, 2.5, 5.0 µmol/L simvastatin, the CD206 expression levels were 68.10% ± 2.48%, 75.28% ± 1.66%, 86.32% ± 2.19% and the secretion of IL-10 (500 ± 5), (675 ± 28) and (916 ± 15) pg/ml respectively. By analysis of variance and q test of mean, the difference was statistically significant (all P < 0.01) between the groups of M1 model (9.67% ± 5.48%, (298 ± 11) pg/ml) . And the phenotypic features were similar to those of the groups of M2 model.
  
  
  CONCLUSIONS
  Simvastatin may inhibit inflammation by enhancing the switching of M1 macrophage to M2 macrophage phenotype.
authors.affiliation: Department of Cardiology, Affiliated Hospital, Guilin Medical College, Guilin 541001, China. Email: drquanzhongli@163.com.
authors.forename: Quan-zhong
authors.initials: QZ
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Jin-jie
authors.initials: JJ
authors.lastname: Han
authors.forename: Zong-jie
authors.initials: ZJ
authors.lastname: Qian
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D007249:Inflammation / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism*; D018664:Interleukin-12 / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D019821:Simvastatin / Q000494:pharmacology*
chemical_list: D016753:Interleukin-10; D018664:Interleukin-12; D019821:Simvastatin