title: Analysis of brazilin and protosappanin B in sappan lignum by capillary zone electrophoresis with acid barrage stacking.
abstract: A method was developed to determine brazilin and protosappanin B in natural products by CE after acid barrage stacking. The optimum conditions were as follows: a BGE of 20 mM sodium tetraborate (pH 9.2) containing 6% v/v of methanol, hydrodynamic injection (0.5 psi, 65 s) followed by hydrodynamic injection of 150 mM citric acid (pH 2.3; 0.5 psi, 22 s), and separated with +25 kV. Under these conditions, brazilin and protosappanin B were separated with a sample-to-sample time less than 13 min and detection limits of 0.28 μg/mL and 0.15 μg/mL, respectively. The applicability of the developed method was demonstrated by the detection of brazilin and protosappanin in methanol extract of sappan lignum.
authors.affiliation: Analysis and Testing Centre, Dezhou University, Dezhou, P. R. China; Institute of Materia Medica, Shandong Academy of Medical Sciences, Jinan, P. R. China; Key Laboratory of Coordination Chemistry and Functional Materials in Universities of Shandong, Dezhou University, Dezhou, P. R. China.
authors.forename: Yuanqi
authors.initials: Y
authors.lastname: Lu
authors.forename: Hong
authors.initials: H
authors.lastname: Bai
authors.forename: Chunyan
authors.initials: C
authors.lastname: Kong
authors.forename: Hao
authors.initials: H
authors.lastname: Zhong
authors.forename: Michael C
authors.initials: MC
authors.lastname: Breadmore
mesh_terms: D001578:Benzopyrans / Q000032:analysis* / Q000737:chemistry; D029911:Caesalpinia / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D001578:Benzopyrans; D004365:Drugs, Chinese Herbal; D010636:Phenols; C044362:brazilin
keywords: Acid barrage stacking (ABS); Brazilin; CE; Protosappanin B