title: Mapping dominant markers using F2 matings.
abstract: The development of efficient methods for amplifying random DNA sequences by the polymerase chain reaction has created the basis for mapping virtually unlimited numbers of mixed-phase dominant DNA markers in one population. Although dominant markers can be efficiently mapped using many different kinds of matings, recombination frequencies and locus orders are often mis-estimated from repulsion F2 matings. The major problem with these matings, apart from excessive sampling errors of recombination frequency (θ) estimates, is the bias of the maximum-likelihood estimator (MLE) of θ (θ ML). [Formula: see text] when the observed frequency of double-recessive phenotypes is 0 and the observed frequency of double-dominant phenotypes is less than 2/3 - the bias for those samples is - θ. We used simulation to estimate the mean bias of θ ML. Mean bias is a function of n and θ and decreases as n increases. Valid maps of dominant markers can be built by using sub-sets of markers linked in coupling, thereby creating male and feamle coupling maps, as long as the maps are fairly dense (about 5 cM) - the sampling errors of θ increase as θ increases for coupling linkages and are equal to those for backcross matings when θ=0. The use of F2 matings for mapping dominant markers is not necessarily proscribed because they yield twice as many useful markers as a backcross population, albeit in two maps, for the same number of DNA extractions and PCR assays; however, dominant markers can be more effeciently exploited by using doubled-haploid, recombinant-inbred, or other inbred populations.
authors.affiliation: Department of Crop and Soil Science, Oregon State University, 97331, Corvallis, OR, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Knapp
authors.forename: J L
authors.initials: JL
authors.lastname: Holloway
authors.forename: W C
authors.initials: WC
authors.lastname: Bridges
authors.forename: B H
authors.initials: BH
authors.lastname: Liu