title: [Dynamics of bone resorption analyzed by intravital imaging].
abstract: By using conventional methods such as histological analysis, many osteoclasts can be observed in the site of bone destruction. However, how the bone-resorptive functions of mature osteoclasts are controlled in vivo remains unclear. To answer this question, we have originally developed an advanced imaging system for visualizing living bone tissues with intravital multiphoton microscopy. Using this system, we succeeded in visualizing bone resorption of mature osteoclasts in living bone. Here we show the latest data and also discuss the further application of intravital bone imaging. This approach would be quite useful for evaluating novel anti-bone-resorptive drugs in vivo .
authors.affiliation: Department of Immunology and Cell Biology, Graduate School of Medicine and Frontier Biosciences, Osaka University, Japan./Japan Science and Technology Agency, CREST, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Kikuta
authors.forename: Masaru
authors.initials: M
authors.lastname: Ishii
mesh_terms: D000818:Animals; D050071:Bone Density Conservation Agents / Q000494:pharmacology / Q000627:therapeutic use; D001862:Bone Resorption / Q000473:pathology*; D001842:Bone and Bones / Q000166:cytology / Q000473:pathology* / Q000648:ultrastructure*; D004195:Disease Models, Animal; D015195:Drug Design; D012968:Etidronic Acid / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton; D057054:Molecular Imaging / Q000379:methods*; D058990:Molecular Targeted Therapy; D010010:Osteoclasts / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure*; D010024:Osteoporosis / Q000188:drug therapy / Q000473:pathology; D053245:RANK Ligand; D000068296:Risedronic Acid
chemical_list: D050071:Bone Density Conservation Agents; D053245:RANK Ligand; D000068296:Risedronic Acid; D012968:Etidronic Acid