title: Facile synthesis of magnetic metal organic frameworks for the enrichment of low-abundance peptides for MALDI-TOF MS analysis.
abstract: In this work, core-shell magnetic metal organic framework (MOF) microspheres were successfully synthesized by coating magnetite particles with mercaptoacetic acid and subsequent reactions with ethanol solutions of Cu(OAc)2 and benzene-1,3,5-tricarboxylic acid (designated as H3 btc) alternately. The resulting Fe3 O4 @[Cu3 (btc)2 ] possess strong magnetic responsiveness. We applied the novel nanocomposites in the enrichment of low-concentration standard peptides, peptides in MYO and BSA tryptic digests and in human urine in combination with MALDI-TOF MS analysis for the first time. In addition, the Cu3 (btc)2 MOF shells exhibit strong affinity to peptides, thus providing a rapid and convenient approach to the concentration of low-abundance peptides. Notably, peptides at an extremely low concentration of 10 pM could be detected by MALDI-TOF MS after enrichment with the magnetic MOF composites. In brief, the facile synthesis and efficient enrichment process of the Fe3 O4 @[Cu3 (btc)2 ] microspheres make them promising candidates for the isolation of peptides in even complex biological environments.
authors.affiliation: Department of Chemistry, Fudan University, Shanghai, China.
authors.forename: Man
authors.initials: M
authors.lastname: Zhao
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Zhang
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
mesh_terms: D000804:Angiotensin II / Q000652:urine; D000818:Animals; D002417:Cattle; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008297:Male; D053761:Nanocomposites / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry; D059748:Proteolysis; D012015:Reference Standards; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods* / Q000592:standards; D013864:Thioglycolates / Q000737:chemistry; D014233:Tricarboxylic Acids / Q000737:chemistry; D014357:Trypsin / Q000737:chemistry
chemical_list: D058185:Magnetite Nanoparticles; D009942:Organometallic Compounds; D010446:Peptide Fragments; D013864:Thioglycolates; D014233:Tricarboxylic Acids; D000804:Angiotensin II; D012710:Serum Albumin, Bovine; C015092:cupric acetate; C017487:2-mercaptoacetate; D014357:Trypsin; C069849:trimesic acid
keywords: Enrichment; Low-abundance peptides; MALDI-TOF MS; Magnetic MOFs microspheres; Nanoproteomics