title: Data-driven quantification of the robustness and sensitivity of cell signaling networks.
abstract: Robustness and sensitivity of responses generated by cell signaling networks has been associated with survival and evolvability of organisms. However, existing methods analyzing robustness and sensitivity of signaling networks ignore the experimentally observed cell-to-cell variations of protein abundances and cell functions or contain ad hoc assumptions. We propose and apply a data-driven maximum entropy based method to quantify robustness and sensitivity of Escherichia coli (E. coli) chemotaxis signaling network. Our analysis correctly rank orders different models of E. coli chemotaxis based on their robustness and suggests that parameters regulating cell signaling are evolutionary selected to vary in individual cells according to their abilities to perturb cell functions. Furthermore, predictions from our approach regarding distribution of protein abundances and properties of chemotactic responses in individual cells based on cell population averaged data are in excellent agreement with their experimental counterparts. Our approach is general and can be used to evaluate robustness as well as generate predictions of single cell properties based on population averaged experimental data in a wide range of cell signaling systems.
authors.affiliation: Battelle Center for Mathematical Medicine, The Research Institute at the Nationwide Children's Hospital, The Ohio State University, 700 Children's Drive, Columbus, OH 43205, USA. Department of Pediatrics, The Ohio State University, 700 Children's Drive, Columbus, OH 43205, USA.
authors.forename: Sayak
authors.initials: S
authors.lastname: Mukherjee
authors.forename: Sang-Cheol
authors.initials: SC
authors.lastname: Seok
authors.forename: Veronica J
authors.initials: VJ
authors.lastname: Vieland
authors.forename: Jayajit
authors.initials: J
authors.lastname: Das
mesh_terms: D002633:Chemotaxis; D019277:Entropy; D004926:Escherichia coli / Q000166:cytology* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism*; D008954:Models, Biological; D015398:Signal Transduction
chemical_list: D029968:Escherichia coli Proteins