title: Effects of PGE2 EP3/EP4 receptors on bladder dysfunction in mice with experimental autoimmune encephalomyelitis.
abstract: To investigate the expression of four subtypes of PGE2 E-prostanoid (EP) receptors (EP1-EP4) and the effects of EP3/EP4 on bladder dysfunction in a new neurogenic bladder model induced by experimental autoimmune encephalomyelitis (EAE), the mouse model of EAE was induced using a previously established method, and bladder function in mice with different defined levels of neurological impairment was then examined, including micturition frequencies and voiding weight. Bladders were then harvested for analysis of EP receptor expression by Western blot. Activities of agonists/antagonists of EP3 and EP4 receptors as well as PGE2 were also evaluated at different stages of EAE. The results showed that EAE mice developed profound bladder dysfunction characterized by significantly increased micturition and significantly decreased urine output per micturition. EAE-induced upregulation of EP3 and EP4 receptors in the bladder was accompanied by bladder dysfunction. However, EAE had no significant effect on EP1 and EP2 receptors. Moreover, PGE2 and agonists/antagonists of EP3 and EP4 receptors significantly affected bladder dysfunction in EAE mice. Thus, we believe that EAE mice are useful for investigations of the neurogenic bladder. In addition, EP3 and EP4 receptors play a role in EAE-induced bladder dysfunction, providing us with a new target for the treatment of neurogenic bladders.
authors.affiliation: Dept. of Urology, The First Affiliated Hospital of Zhengzhou Univ., No.1 Jian She Dong Ave., Zhengzhou 450002, People's Republic of China. msl197893@126.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Xue
authors.forename: Zhankui
authors.initials: Z
authors.lastname: Jia
authors.forename: Xiaomu
authors.initials: X
authors.lastname: Kong
authors.forename: Guofu
authors.initials: G
authors.lastname: Pi
authors.forename: Shengli
authors.initials: S
authors.lastname: Ma
authors.forename: Jinjian
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000503:physiopathology*; D005260:Female; D051379:Mice; D009929:Organ Size / Q000502:physiology; D018078:Receptors, Prostaglandin E / Q000502:physiology*; D058308:Receptors, Prostaglandin E, EP3 Subtype / Q000502:physiology*; D058309:Receptors, Prostaglandin E, EP4 Subtype / Q000502:physiology*; D001743:Urinary Bladder / Q000503:physiopathology*; D053201:Urinary Bladder, Overactive / Q000503:physiopathology; D014554:Urination / Q000502:physiology
chemical_list: C545648:Ptger3 protein, mouse; C545651:Ptger4 protein, mouse; D018078:Receptors, Prostaglandin E; D058308:Receptors, Prostaglandin E, EP3 Subtype; D058309:Receptors, Prostaglandin E, EP4 Subtype
keywords: E-prostanoid receptors; experimental autoimmune encephalomyelitis; neurogenic bladder; prostaglandin E2