title: Preliminary study on diffuse axonal injury by Fourier transform infrared spectroscopy histopathology imaging.
abstract: The objective of this study was to evaluate the application of Fourier transform infrared (FTIR) spectroscopy for detecting diffuse axonal injury (DAI) in a mouse model. Brain tissues from DAI mouse model were prepared with H&E, silver, and β-amyloid precursor protein (β-APP) immunohistochemistry stains and were also studied with FTIR. The infrared spectrum images showed high absorption of amide II in the subcortical white matter of the experimental mouse brain, while there was no obvious expression of amide II in the control mouse brain. The areas with high absorption of amide II were in the same distribution as the DAI region confirmed by the silver and β-APP studies. The result suggests that high absorption of amide II correlates with axonal injury. The use of FTIR imaging allows the biochemical changes associated with DAI pathologies to be detected in the tissues, thus providing an important adjunct method to the current conventional pathological diagnostic techniques.
authors.affiliation: Key Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, 25 West Tucheng Road, Haidian District, Beijing, 100088, China.
authors.forename: Tiantong
authors.initials: T
authors.lastname: Yang
authors.forename: Guanglong
authors.initials: G
authors.lastname: He
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Chang
authors.forename: Haidong
authors.initials: H
authors.lastname: Zhang
authors.forename: Mary G
authors.initials: MG
authors.lastname: Ripple
authors.forename: David R
authors.initials: DR
authors.lastname: Fowler
authors.forename: Ling
authors.initials: L
authors.lastname: Li
mesh_terms: D000577:Amides / Q000737:chemistry; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D020833:Diffuse Axonal Injury / Q000473:pathology*; D007150:Immunohistochemistry; D051379:Mice; D023421:Models, Animal; D009410:Nerve Degeneration / Q000473:pathology; D009474:Neurons / Q000473:pathology; D017550:Spectroscopy, Fourier Transform Infrared; D013194:Staining and Labeling
chemical_list: D000577:Amides; D016564:Amyloid beta-Protein Precursor
keywords: Fourier transform infrared spectroscopy; animal study; diffuse axonal injury; forensic pathology; forensic science; traumatic brain injury