title: Conjugation of peptide nucleic acid with a pyrrole/imidazole polyamide to specifically recognize and cleave DNA.
abstract: Cut loose: A pseudocomplementary peptide nucleic acid was tethered to a pyrrole/imidazole hairpin polyamide, and was used to selectively target a specific DNA sequence. Binding even occurs under high salt conditions. Furthermore, the conjugate facilitated sequence-specific scission of long dsDNA. This simple approach promises to resolve the technical difficulties in targeting DNA sequences with PNA.
authors.affiliation: Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904 (Japan).
authors.forename: Wataru
authors.initials: W
authors.lastname: Kameshima
authors.forename: Takumi
authors.initials: T
authors.lastname: Ishizuka
authors.forename: Masafumi
authors.initials: M
authors.lastname: Minoshima
authors.forename: Makoto
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Makoto
authors.initials: M
authors.lastname: Komiyama
mesh_terms: D000577:Amides / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D053837:DNA Cleavage; D007093:Imidazoles / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
chemical_list: D000577:Amides; D007093:Imidazoles; D020135:Peptide Nucleic Acids; D011758:Pyrroles; C029899:imidazole; D004247:DNA
keywords: DNA cleavage; DNA recognition; amides; noncovalent interactions; peptide nucleic acids