title: Phylogenetic analysis of canine parvovirus partial VP2 gene in India.
abstract: A total of 85 samples (58.0 %) were found to be positive for Canine parvovirus (CPV) by PCR assay (Hfor/Hrev primers) out of 158 suspected faecal samples of dogs collected from various states/union territories of India. Nine CPV isolates could be obtained in A-72 cell line. The sequencing of the partial VP2 gene of CPV identified the predominant CPV strain as CPV-2a (Ser297Ala) with one CPV-2b (Ser297Ala) and another CPV-2a variant strain (Ser297Gly). Several non-synonymous and synonymous mutations were also recorded in this study. The phylogenetic tree revealed that most of the CPV sequences from Tamil Nadu (Southern India) and Maharashtra (Western India) obtained during 2011 and few sequences from Northern India obtained during 2012 were grouped together along with CPV-2a (Ser297Ala) strains from China and India and followed the same evolution; although there was definitive indication of separate lineages too by few other sequences.
authors.affiliation: Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry, 605 009, India, mhirak@rediffmail.com.
authors.forename: H K
authors.initials: HK
authors.lastname: Mukhopadhyay
authors.forename: Samyukta Lakshmi
authors.initials: SL
authors.lastname: Matta
authors.forename: S
authors.initials: S
authors.lastname: Amsaveni
authors.forename: P X
authors.initials: PX
authors.lastname: Antony
authors.forename: J
authors.initials: J
authors.lastname: Thanislass
authors.forename: R M
authors.initials: RM
authors.lastname: Pillai
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D004279:DNA, Viral / Q000235:genetics; D004285:Dogs; D005243:Feces / Q000821:virology*; D014644:Genetic Variation; D007194:India; D008969:Molecular Sequence Data; D017993:Parvovirus, Canine / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D017354:Point Mutation; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D015678:Viral Structural Proteins