title: Proteomic identification of rainbow trout seminal plasma proteins.
abstract: In the study, the combination of protein fractionation by 1DE and HPLC-ESI-MS/MS was used to characterize the rainbow trout seminal plasma proteome. Our results led to the creation of a catalogue of rainbow trout seminal plasma proteins (152 proteins) and significantly contributed to the current knowledge regarding the protein composition of fish seminal plasma. The major proteins of rainbow trout seminal plasma, such as transferrin, apolipoproteins, complement C3, serum albumin, and hemopexin-, alpha-1-antiproteinase-, and precerebellin-like protein, were recognized as acute-phase proteins (proteins that plasma concentration changes in response to inflammation). This study provides the basis for further functional studies of fish seminal plasma proteins, as well as for the identification of novel biomarkers for sperm quality. The MS data have been deposited in the ProteomeXchange with identifier PXD000306 (http://proteomecentral.proteomexchange.org/dataset/PXD000306).
authors.affiliation: Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Nynca
authors.forename: Georg J
authors.initials: GJ
authors.lastname: Arnold
authors.forename: Thomas
authors.initials: T
authors.lastname: Fröhlich
authors.forename: Kathrin
authors.initials: K
authors.lastname: Otte
authors.forename: Florian
authors.initials: F
authors.lastname: Flenkenthaler
authors.forename: Andrzej
authors.initials: A
authors.lastname: Ciereszko
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D029941:Fish Proteins / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D008297:Male; D017686:Oncorhynchus mykiss; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics; D029589:Seminal Plasma Proteins / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D053719:Tandem Mass Spectrometry
chemical_list: D029941:Fish Proteins; D020543:Proteome; D029589:Seminal Plasma Proteins
keywords: Animal proteomics; MS; Rainbow trout; Seminal plasma