title: Protein tyrosine phosphatase profiling studies during brown adipogenic differentiation of mouse primary brown preadipocytes.
abstract: There is a correlation between obesity and the amount of brown adipose tissue; however, the molecular mechanism of brown adipogenic differentiation has not been as extensively studied. In this study, we performed a protein tyrosine phosphatase (PTP) profiling analysis during the brown adipogenic differentiation of mouse primary brown preadipocytes. Several PTPs, including PTPRF, PTPRZ, and DUSP12 showing differential expression patterns were identified. In the case of DUSP12, the expression level is dramatically downregulated during brown adipogenesis. The ectopic expression of DUSP12 using a retroviral expression system induces the suppression of adipogenic differentiation, whereas a catalytic inactive DUSP12 mutant showed no effect on differentiation. These results suggest that DUSP12 is involved in brown adipogenic differentiation and may be used as a target protein for the treatment or prevention of obesity by the regulation of brown adipogenic differentiation.
authors.affiliation: Research Center for Integrated Cellulomics, KRIBB; Department of Functional Genomics, University of Science and Technology (UST), Daejeon 305-806, Korea khbae@kribb.re.kr.
authors.forename: Hye-Ryung
authors.initials: HR
authors.lastname: Choi
authors.forename: Won Kon
authors.initials: WK
authors.lastname: Kim
authors.forename: Anna
authors.initials: A
authors.lastname: Park
authors.forename: Hyeyun
authors.initials: H
authors.lastname: Jung
authors.forename: Baek Soo
authors.initials: BS
authors.lastname: Han
authors.forename: Sang Chul
authors.initials: SC
authors.lastname: Lee
authors.forename: Kwang-Hee
authors.initials: KH
authors.lastname: Bae
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis; D002001:Adipose Tissue, Brown / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D015536:Down-Regulation; D054637:Dual-Specificity Phosphatases / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D009154:Mutation; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000378:metabolism*
chemical_list: C489855:dual specificity phosphatase 12; D054637:Dual-Specificity Phosphatases; D017027:Protein Tyrosine Phosphatases