title: Activator protein-1 (AP-1) and response to pathogen infection in the Hong Kong oyster (Crassostrea hongkongensis).
abstract: Growing evidence suggests that the transcription factor activator protein-1 (AP-1), a downstream target of mitogen-activated protein kinase (MAPK) signaling, plays a major role in stimulating the synthesis of immune effector molecules during innate immune responses. We have characterized ChAP-1, an AP-1-like protein in Crassostrea hongkongensis that is a member of the AP-1 family of proteins. ChAP-1 is composed of 290 amino acid residues with a Jun and bZIP domain at the N- and C-termini, respectively, a structure similar to that of known Ap-1 proteins. ChAP-1 mRNA is expressed in several tissues analyzed, with highest expression in the mantle. Expression of ChAP-1 increases in response to Vibrio alginolyticus, Salmo haemolyticus or Salmo cerevisiae infection and, despite the location of GFP-tagged full-length ChAP-1 protein in the cytoplasm, ChAP-1 activates the transcription of an L8G5-luc reporter gene, and its over-expression can also activate the AP-1-Luc reporter gene in HEK293T cells.
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, Guangdong, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Xiang
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, Guangdong, China; Graduate School of the Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.
authors.forename: Fufa
authors.initials: F
authors.lastname: Qu
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, Guangdong, China; Graduate School of the Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Qi
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, Guangdong, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Yang
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, Guangdong, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Kong
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, Guangdong, China. Electronic address: carlzyu@scsio.ac.cn.
authors.forename: Ziniu
authors.initials: Z
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D049896:Crassostrea / Q000235:genetics / Q000276:immunology* / Q000821:virology; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010802:Phylogeny; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000276:immunology*; D014162:Transfection / Q000379:methods; D014735:Vibrio Infections / Q000276:immunology* / Q000821:virology; D044149:Vibrio alginolyticus / Q000276:immunology*
chemical_list: D018808:Transcription Factor AP-1; D012313:RNA
keywords: ChAP-1; Crassostrea hongkongensis; Immunity