title: Apigenin potentiates the growth inhibitory effects by IKK-β-mediated NF-κB activation in pancreatic cancer cells.
abstract: Apigenin is a potential chemopreventive agent for cancer prevention. Because of the central role of transcription factor nuclear factor-κB (NF-κB) in pancreatic cancer, we investigated the roles of NF-κB in apigenin-induced growth inhibition in pancreatic cancer cells. It showed that apigenin reduced cell growth and induced apoptosis in the cells. Apigenin treatment down-regulated not only basal but also TNF-α-induced NF-κB DNA binding activity, NF-κB transcription activity, inhibitor of κB (IκB)-α phosphorylation together with translocation of p65 and p50, and it accompanied with the blockade of IκB kinase (IKK)-β activity. Moreover, IKK blockage potentiated the anticancer efficacy of apigenin and IKK-β overexpression attenuated the apigenin-induced cell growth inhibition. Additionally, apigenin (30 mg/kg) administration suppressed pancreatic cancer growth and IKK-β activation in nude mice xenograft. These results indicated that apigenin had a potential to inhibit IKK-β-mediated NF-κB activation, and was a valuable agent for the pancreatic cancer treatment.
authors.affiliation: Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Ding-Guo
authors.initials: DG
authors.lastname: Wu
authors.forename: Peng
authors.initials: P
authors.lastname: Yu
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Jiang
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Huai-Zhi
authors.initials: HZ
authors.lastname: Wang
authors.forename: Lei-Da
authors.initials: LD
authors.lastname: Zhang
authors.forename: Ming-Bo
authors.initials: MB
authors.lastname: Wen
authors.forename: Ping
authors.initials: P
authors.lastname: Bie
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000818:Animals; D047310:Apigenin / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004247:DNA / Q000378:metabolism; D005260:Female; D006801:Humans; D051550:I-kappa B Kinase / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000188:drug therapy* / Q000473:pathology
chemical_list: D016328:NF-kappa B; D047310:Apigenin; D004247:DNA; D051550:I-kappa B Kinase
keywords: Apigenin; IKK-β; NF-κB; Pancreatic cancer