title: Global long interspersed nuclear element 1 DNA methylation in a Colombian sample of patients with late-onset Alzheimer's disease.
abstract: Alterations in DNA methylation have implicated as an epigenetic event in the pathogenesis of late-onset Alzheimer's disease (LOAD). The objective of this work was to evaluate global DNA methylation levels for long interspersed nuclear element 1 (LINE-1) repetitive sequences in Colombian patients with LOAD and controls. The LINE-1 DNA methylation levels in peripheral blood samples from 28 Colombian patients with LOAD and 30 healthy participants were assessed using a methylation-sensitive high-resolution melting (MS-HRM) quantitative assay. We did not find differences in LINE-1 methylation levels between patients with Alzheimer's disease (AD; median 76.2%, interquartile range [IQR]: 69.8-81.9) and control participants (median 79.8%, IQR: 73.2-83.8; P = .3). Additional stratified analyses did not show differences in LINE-1 methylation levels for male or female patients versus controls nor for apolipoprotein E4 carriers and noncarriers. This is the first report of LINE-1 methylation levels in patients with LOAD using the cost-effective MS-HRM technique, and this is the first global DNA methylation study in Latin American patients with AD.
authors.affiliation: 1Laboratory of Neuropsychiatric Genetics, Biomedical Sciences Research Group, School of Medicine, Universidad Antonio Nariño, Bogotá, Colombia.
authors.forename: Hernán G
authors.initials: HG
authors.lastname: Hernández
authors.forename: María F
authors.initials: MF
authors.lastname: Mahecha
authors.forename: Adriana
authors.initials: A
authors.lastname: Mejía
authors.forename: Humberto
authors.initials: H
authors.lastname: Arboleda
authors.forename: Diego A
authors.initials: DA
authors.lastname: Forero
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000235:genetics*; D016022:Case-Control Studies; D003105:Colombia; D019175:DNA Methylation / Q000235:genetics*; D005260:Female; D006801:Humans; D016014:Linear Models; D020084:Long Interspersed Nucleotide Elements / Q000235:genetics*; D008297:Male
keywords: DNA methylation; LINE-1; Latin America; MS-HRM; epigenetics; late-onset Alzheimer’s disease; repetitive sequences