title: HPLC-DAD-ESI-MS analysis of phenolic compounds during ripening in exocarp and mesocarp of tomato fruit.
abstract: Identification of phenolic compounds was done by means of liquid chromatography (HPLC) coupled to mass spectrometry (MS) using the electrospray ionization interface (ESI). Quantification of phenolic compounds was carried out by using HPLC with diode array detector (DAD) in exocarp and mesocarp of tomato fruit at 6 different ripeness stages (mature-green, breakers, turning, pink, light-red, and red). Several phenolic compounds were identified including chlorogenic acid, caffeic acid, p-coumaric acid, ferulic acid, and rutin and some combined phenolic acids were tentatively identified, mainly glycosides, such as caffeoyl hexose I, caffeoyl hexose II, caffeoylquinic acid isomer, dicaffeoylquinic acid, p-coumaroyl hexose I, p-coumaroyl hexose II, feruloyl hexose I, feruloyl hexose II, siringyl hexose, and caffeoyl deoxyhexose hexose. Fruit exocarp had higher quantities of total soluble phenolics (TSP) compared to mesocarp. During ripening, TSP increased in both exocarp and mesocarp, mainly in exocarp. While rutin increased, chlorogenic acid decreased in both tissues: exocarp and mesocarp.
authors.affiliation: Maestría en Ciencia y Tecnología de Alimentos, Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, 80000, México.
authors.forename: Armando
authors.initials: A
authors.lastname: Carrillo-López
authors.forename: Elhadi
authors.initials: E
authors.lastname: Yahia
mesh_terms: D002109:Caffeic Acids / Q000032:analysis; D002726:Chlorogenic Acid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000032:analysis; D005638:Fruit / Q000737:chemistry; D006027:Glycosides / Q000032:analysis; D062385:Hydroxybenzoates / Q000032:analysis; D018551:Solanum lycopersicum / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D011422:Propionates; D012431:Rutin / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D002109:Caffeic Acids; D003373:Coumaric Acids; D006027:Glycosides; D062385:Hydroxybenzoates; D010636:Phenols; D011422:Propionates; D002726:Chlorogenic Acid; D012431:Rutin; C004999:ferulic acid; C017616:phenolic acid; C495469:p-coumaric acid; C040048:caffeic acid
keywords: Licopersicon esculentum; TOF-MS; caffeic acid; chlorogenic acid; rutin