title: [Effect of 0.1 Gy X-ray irradiation on gene expression profiles in normal human lymphoblastoid cells].
abstract: OBJECTIVE
  To investigate the effect of 0.1 Gy X-ray irradiation on the gene expression profiles in normal human lymphoblastoid cells using gene microarray and to explore the possible mechanism of the biological effect of low-dose irradiation.
  
  
  METHODS
  The NimbleGen 12×135 K microarray corresponding to 45033 genes was used to analyze the gene expression profiles in AHH-1 cells cultured for 6 h and 20 h after 0.1 Gy X-ray irradiation. A gene was identified as the differentially expressed gene if the ratio between its expression levels in irradiation group and control group was higher than 2 or lower than 0.5. RT-PCR and real-time PCR were used to confirm some differentially expressed genes.
  
  
  RESULTS
  There were 760 up-regulated genes and 1222 down-regulated genes in the cells at 6 h after 0.1 Gy X-ray irradiation, while there were 463 up-regulated genes and 753 down-regulated genes at 20 h after 0.1 Gy X-ray irradiation; there were 92 differentially expressed genes in common. The expression of GADD45A, CDKN2A, and Cx43 measured using gene microarray was confirmed by RT-PCR and real-time PCR.
  
  
  CONCLUSIONS
  Low-dose irradiation can affect the expression of many functional genes, which provides a basis for the research on the mechanism of radiation damage.
authors.affiliation: Institute of Radiation Medicine, Shandong Academy of Medical Science, Jinan 250062, China. E-mail: stx90@163.com.
authors.forename: Jie-qing
authors.initials: JQ
authors.lastname: Li
authors.forename: Dian-jun
authors.initials: DJ
authors.lastname: Hou
authors.forename: Ya
authors.initials: Y
authors.lastname: Ma
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.forename: Wei-guo
authors.initials: WG
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Jian-wei
authors.initials: JW
authors.lastname: Qiao
authors.forename: Xi-ming
authors.initials: XM
authors.lastname: Jia
authors.forename: Kun
authors.initials: K
authors.lastname: Li
mesh_terms: D002460:Cell Line; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects*; D020411:Oligonucleotide Array Sequence Analysis; D011829:Radiation Dosage; D011839:Radiation, Ionizing; D059467:Transcriptome; D014965:X-Rays