title: Comparison of the performance of carcinogenic HPV typing of the Roche Linear Array and Qiagen LiquiChip® HPV assays.
abstract: BACKGROUND
  Cervical cancer is caused by high-risk types of human papillomavirus (HPV). DNA testing of such high-risk types of HPV could improve cervical screening.The aim of the study was to compare the sensitivities and positive predictive values of two commercially available typing assays (Qiagen LQ and Roche LA) and to comparatively assess the distribution of HPV types with these two assays.
  
  
  METHODS
  The study population comprised 311 ASCUS + women with abnormal pap tests who were HCII positive and who were admitted to three European referral gynecology clinics between 2007 and 2010 (Madrid, Marseille and Milan). All patients underwent LQ and LA tests.
  
  
  RESULTS
  The sensitivity of the two assays for HPV typing was 94% for LQ and 99% for LA (compared with HCII). The overall concordance between LQ and LA was 93%. The three prevalent genotypes, HPV16, HPV18, and HPV31, were identified with a high concordance using the two assays: kappa 0.93, 0.83, and 0.91, respectively. Mixed genotypes were more frequently detected by LA than by LQ: 52% vs. 18%, respectively (p < .0001).
  
  
  CONCLUSIONS
  These assays have a good clinical sensitivity for detecting HPV types in CIN2+ patients and allow the virus type to be detected in the same experiment. Our study revealed no significant difference between LQ and LA for CIN2+ or CIN3+ diagnosis, indicating similar distributions of HPV types and a mixed genotype detection that is higher for LA than for LQ.
authors.forename: Philippe
authors.initials: P
authors.lastname: Halfon
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Sandri
authors.forename: Audrey
authors.initials: A
authors.lastname: Raimondo
authors.forename: Sophie
authors.initials: S
authors.lastname: Ravet
authors.forename: Hacène
authors.initials: H
authors.lastname: Khiri
authors.forename: Mario
authors.initials: M
authors.lastname: Sideri
authors.affiliation: Laboratoire Alphabio, 1 rue Melchior Guinot, Marseille 13003, France. g.penaranda@alphabio.fr.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Penaranda
authors.forename: Claire
authors.initials: C
authors.lastname: Camus
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Mateos Lindemann
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005260:Female; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods; D006801:Humans; D008875:Middle Aged; D058889:Molecular Typing / Q000379:methods; D027383:Papillomaviridae / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000821:virology*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000821:virology; D055815:Young Adult
chemical_list: D004279:DNA, Viral