title: Bifidobacterium moukalabense sp. nov., isolated from the faeces of wild west lowland gorilla (Gorilla gorilla gorilla).
abstract: Gram-staining-positive anaerobic rods were isolated from the faeces of a wild lowland gorilla (Gorilla gorilla gorilla) in Moukalaba-Doudou National Park, Gabon, and strain GG01(T) was taxonomically investigated. Based on phylogenetic analyses and specific phenotypic characteristics, the strain belonged to the genus Bifidobacterium. Phylogenetic analysis of its 16S rRNA gene sequence revealed that strain GG01(T) formed a single monophyletic cluster and had a distinct line of descent. Based on 16S rRNA gene sequence similarity, the type strains of Bifidobacterium catenulatum JCM 1194(T) (98.3%) and Bifidobacterium pseudocatenulatum (98.1%) JCM 1200(T) were the most closely related to this novel strain, although it was clear that they belonged to different species. hsp60 sequences also supported these relationships. The DNA G+C content of this novel strain was 60.1 mol%. Bifidobacterium moukalabense sp. nov. (type strain GG01(T) = JCM 18751(T) = DSM 27321(T)) is proposed.
authors.affiliation: Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Shimogamo, Kyoto 606-8522, Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Tsuchida
authors.affiliation: Techno-Suruga Laboratory Co., Ltd, Shizuoka 424-0065, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Takahashi
authors.affiliation: Research Institute of Tropical Ecology, Libreville, Bp 13354, Gabon.
authors.forename: Pierre Philippe Mbehang
authors.initials: PPM
authors.lastname: Nguema
authors.affiliation: Graduate School of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
authors.forename: Shiho
authors.initials: S
authors.lastname: Fujita
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Kitahara
authors.affiliation: Graduate School of Science, Kyoto University, Kitashirakawa, Kyoto 606-8502, Japan.
authors.forename: Juichi
authors.initials: J
authors.lastname: Yamagiwa
authors.affiliation: Research Institute of Tropical Ecology, Libreville, Bp 13354, Gabon.
authors.forename: Alfred
authors.initials: A
authors.lastname: Ngomanda
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Moriya
authors.initials: M
authors.lastname: Ohkuma
authors.affiliation: Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Shimogamo, Kyoto 606-8522, Japan.
authors.forename: Kazunari
authors.initials: K
authors.lastname: Ushida
mesh_terms: D000818:Animals; D001482:Base Composition; D001644:Bifidobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D018834:Chaperonin 60 / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005243:Feces / Q000382:microbiology*; D005681:Gabon; D005798:Genes, Bacterial; D006071:Gorilla gorilla / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D018834:Chaperonin 60; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S