title: [Expression of CD90/EpCAM/CD24 in hepatocellular carcinoma cell lines at various stages of differentiation].
abstract: OBJECTIVE
  To confirm the malignant phenotype of hepatocarcinoma cell (HCC) lines at various stages of differentiation (MHCC97L, MHCC97H and HCCLM3) and to explore their expression levels of cancer stem cell (CSC) markers.
  
  
  METHODS
  The invasive and proliferative properties of each HCC line were assessed by transwell assay and the Cell Counting Kit-8 (CCK-8) colorimetric assay. Sensitivity to chemotherapy was assessed by treatment with oxaliplatin and determination of the half inhibitory concentration (IC50). The expression of CD90, EpCAM and CD24 was measured by flow cytometry.
  
  
  RESULTS
  The number of cells that migrated through the invasion assay membrane were significantly different between the three HCC lines: HCCLM3 (30.57 +/- 8.95) more than MHCC97H (21.33 +/- 4.17) more than HCC97L (9.33 +/- 3.85), P less than 0.01. The IC50 was significantly different between the three HCC lines: HCCLM3 (36.57 +/- 6.95) mumol/L more than MHCC97H (26.35+/-3.88) mumol/L more than MHCC97L (17.68 +/- 3.25) mumol/L. The CSC marker with the highest expression on all three HCC lines was CD90 (HCCLM3: 0.92% +/- 0.21%, MHCC97H: 1.98% +/- 0.23%, and MHCC97L: 2.55% +/- 0.34%), followed by EpCAM (2.11% +/- 0.32%, 3.23% +/- 0.18%, and 4.38% +/-0.49%, respectively), and CD24 as the lowest (0.68% +/- 0.37%, 1.22% +/- 0.26%, and 1.36% +/- 0.24%, respectively).
  
  
  CONCLUSIONS
  Higher expression of CSC markers on HCC lines is associated with a stronger invasive ability and higher sensitivity to chemotherapy.
authors.affiliation: Department of Hepatobiliary Surgery, the General Hospital of Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Ben-dong
authors.initials: BD
authors.lastname: Chen
authors.forename: Yong-feng
authors.initials: YF
authors.lastname: Hui
authors.forename: Hai-bin
authors.initials: HB
authors.lastname: Zhang
authors.forename: Lu-feng
authors.initials: LF
authors.lastname: Fan
authors.forename: Ma-hui
authors.initials: MH
authors.lastname: Si
authors.forename: Guang-shun
authors.initials: GS
authors.lastname: Yang
mesh_terms: D000951:Antigens, Neoplasm / Q000378:metabolism; D051927:CD24 Antigen / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D000071858:Epithelial Cell Adhesion Molecule; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000378:metabolism*; D015398:Signal Transduction; D018800:Thy-1 Antigens / Q000378:metabolism
chemical_list: D000951:Antigens, Neoplasm; D051927:CD24 Antigen; D015815:Cell Adhesion Molecules; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule; D018800:Thy-1 Antigens