title: Alleviation of transient global ischemia/reperfusion-induced brain injury in rats with 1,2,3,4,6-penta-O-galloyl-β-d-glucopyranose isolated from Mangifera indica.
abstract: Present study was undertaken to evaluate the protective effect of 1,2,3,4,6-penta-O-galloyl-β-d-glucopyranose (PGG) against transient global ischemia/reperfusion (I/R)-induced brain injury in rats. Sixty minutes of global ischemia, followed by 24h of reperfusion caused significant alterations in cognition and memory (p<0.01), significant deterioration of motor coordination, grip strength, and limb tone (P<0.01) associated with neurological deficit. In addition, significant decrease in catalase (P<0.01), and superoxide dismutase (SOD) (P<0.01) activities, increase in lipid peroxidation (P<0.01), depletion of reduced glutathione (GSH) (P<0.01), and increase in brain volume (P<0.01) was observed. Additionally, I/R insult has aggravated the cerebral infarct formation (P<0.01), and the histopathology of brain showed congestion of blood vessels, edema of brain parenchyma, leukocyte infiltration as signs of neuroinflammation, and necrosis of brain tissue. Interestingly, pre-treatment with quercetin (20mg/kg, i.p.), and PGG (5 and 10mg/kg, i.p.) for 7 days showed significant, and dose dependent protection against I/R-induced brain injury by alleviating all the behavioral, neurological, morphological, and histological changes induced by I/R. Besides, PGG is a well-known antioxidant, and its protective effect against I/R-induced brain injury is thought to be due to its potent antioxidant property.
authors.affiliation: Department of Pharmacology, PES College of Pharmacy, Hanumanthanagar, Bangalore 560050, Karnataka, India. Electronic address: glv_000@yahoo.com.
authors.forename: G L
authors.initials: GL
authors.lastname: Viswanatha
authors.forename: H
authors.initials: H
authors.lastname: Shylaja
authors.forename: C G
authors.initials: CG
authors.lastname: Mohan
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D001930:Brain Injuries / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000523:psychology; D002374:Catalase / Q000378:metabolism; D018737:Hand Strength; D047348:Hydrolyzable Tannins / Q000494:pharmacology / Q000627:therapeutic use*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D031022:Mangifera; D018782:Maze Learning / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D004856:Postural Balance / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000523:psychology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D047348:Hydrolyzable Tannins; D018696:Neuroprotective Agents; C435084:pentagalloylglucose; D002374:Catalase; D013482:Superoxide Dismutase
keywords: 1,2,3,4, 6-Penta-O-galloyl-β-d-glucopyranose; Ischemia/reperfusion; Neuroprotection; Oxidative stress; Stroke