title: Association of Thr241Met polymorphism of XRCC3 gene with risk of colorectal cancer in the Polish population.
abstract: DNA double strand breaks (DSBs) are the most dangerous lesions which can lead to carcinogenesis. Homologous recombination (HR) is an important pathway responsible for maintaining genome integrity through repair of DSBs. Single nucleotide polymorphism (SNP) is an essential source of genetic variation whose presence in genes involved in HR may have a crucial role in modulation of DNA repair capacity. This case-control study was designed to evaluate the influence of XRCC3 gene Thr241Met polymorphism on CRC risk and progression among Polish population. Genotyping was performed by RFLP-PCR (restriction length fragment polymorphism). The subject of our study was consist of 194 patients with CRC and 204 cancer-free individuals who were age and sex-matched as a control group. Obtained genotype distributions in controls as well as patients fit to the Hardy-Weinberg expectations. Odd ratio analysis indicates diminished risk for heterozygous model and Met allele. Comparison of patients with noninvasive and advanced stage of CRC did not imply any statistical significance. Our results suggest that Thr241Met XRCC3 gene polymorphism might be regarded as CRC potential molecular marker. Nevertheless, that hypothesis needs to be confirmed by subsequent studies.
authors.affiliation: Ireneusz Majsterek PhD, Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, e-mail: ireneusz.majsterek@umed.lodz.pl.
authors.forename: Bartosz
authors.initials: B
authors.lastname: Mucha
authors.forename: Karolina
authors.initials: K
authors.lastname: Przybylowska-Sygut
authors.forename: Adam Janusz
authors.initials: AJ
authors.lastname: Dziki
authors.forename: Lukasz
authors.initials: L
authors.lastname: Dziki
authors.forename: Andrzej
authors.initials: A
authors.lastname: Sygut
authors.forename: Ireneusz
authors.initials: I
authors.lastname: Majsterek
mesh_terms: D015179:Colorectal Neoplasms / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D011044:Poland; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D004268:DNA-Binding Proteins; C110901:X-ray repair cross complementing protein 3