title: Anaerobic cellulolytic rumen fungal populations in goats fed with and without Leucaena leucocephala hybrid, as determined by real-time PCR.
abstract: The effect of Leucaena leucocephala hybrid-Bahru (LLB), which contains a high concentration of condensed tannins, on cellulolytic rumen fungal population in goats was investigated using real-time PCR. The fungal population in goats fed LLB was inhibited during the first 10 days of feeding, but after 15 days of feeding, there was a tremendous increase of fungal population (157.0 μg/ml), which was about fourfold more than that in control goats (39.7 μg/ml). However, after this period, the fungal population decreased continuously, and at 30 days of feeding, the fungal population (50.6 μg/ml) was not significantly different from that in control goats (55.4 μg/ml).
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, Selangor, 43400, Malaysia.
authors.forename: Ching Mun
authors.initials: CM
authors.lastname: Kok
authors.forename: Chin Chin
authors.initials: CC
authors.lastname: Sieo
authors.forename: Hui Yin
authors.initials: HY
authors.lastname: Tan
authors.forename: Wan Zuhainis
authors.initials: WZ
authors.lastname: Saad
authors.forename: Juan Boo
authors.initials: JB
authors.lastname: Liang
authors.forename: Yin Wan
authors.initials: YW
authors.lastname: Ho
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D058448:Biota; D002482:Cellulose / Q000378:metabolism; D004032:Diet / Q000379:methods; D007887:Fabaceae / Q000378:metabolism; D005658:Fungi / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D006041:Goats; D006868:Hydrolysis; D060888:Real-Time Polymerase Chain Reaction; D012417:Rumen / Q000382:microbiology*
chemical_list: D002482:Cellulose