title: Isolation and characterization of a novel wheat cysteine-rich receptor-like kinase gene induced by Rhizoctonia cerealis.
abstract: Cysteine-rich receptor kinases (CRKs) belong to the receptor-like kinase family. Little is known about CRK genes in wheat. We isolated a wheat CRK gene TaCRK1 from Rhizoctonia cerealis-resistant wheat CI12633 based on a differentially expressed sequence identified by RNA-Sequencing (RNA-Seq) analysis. TaCRK1 was more highly expressed in CI12633 than in susceptible Wenmai 6. Transcription of TaCRK1 in wheat was induced in CI12633 after R. cerealis infection and exogenous abscisic acid (ABA) treatment. The deduced TaCRK1 protein contained a signal peptide, two DUF26 domains, a transmembrane domain, and a serine/threonine protein kinase domain. Transient expression of a green fluorescence protein fused with TaCRK1 in wheat and onion indicated that TaCRK1 may localize to plasma membranes. Characterization of TaCRK1 silencing induced by virus-mediated method in CI12633 showed that the downregulation of TaCRK1 transcript did not obviously impair resistance to R. cerealis. This study paves the way to further CRK research in wheat.
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Rong
authors.forename: Lin
authors.initials: L
authors.lastname: Qi
authors.forename: Jiarui
authors.initials: J
authors.lastname: Li
authors.forename: Xuening
authors.initials: X
authors.lastname: Wei
authors.forename: Zengyan
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism; D060467:Disease Resistance / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D020868:Gene Silencing; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics*; D021381:Protein Transport; D012232:Rhizoctonia; D016415:Sequence Alignment; D014158:Transcription, Genetic; D014908:Triticum / Q000145:classification / Q000235:genetics* / Q000382:microbiology*
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins; D011494:Protein Kinases