title: mRNA expression of MMP-28 (Epilysin) in gingival tissues of chronic and aggressive periodontitis patients: a reverse transcriptase PCR study.
abstract: OBJECTIVE
  Matrix metalloproteinases degrade extracellular membrane and also release bioactive fragments and growth factors, thus influencing fundamental biological and pathological processes. Epilysin (MMP-28) differs from most other MMPs as it is expressed in a number of normal tissues, suggestive of functions in tissue homeostasis. The aim of the present study was to quantitatively evaluate and compare the mRNA expression of epilysin (MMP-28) in gingival tissues of healthy patients and of patients affected by chronic or aggressive periodontitis.
  
  
  METHODS
  A total of 60 subjects, 20 periodontally healthy subjects, 20 with chronic periodontitis, and 20 with aggressive periodontitis, were included in this study. Periodontal status was evaluated by measuring gingival index, probing depth and clinical attachment level. mRNA expression of MMP-28 was determined by quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) in gingival tissue samples collected.
  
  
  RESULTS
  Relative quantification of mRNA expression of MMP-28 was highest in healthy tissues (RQ = 0.97) when compared to subjects with chronic periodontitis (RQ = 0.37) and aggressive periodontitis (RQ = 0.23), but the difference was not statistically significant.
  
  
  CONCLUSIONS
  mRNA expression of MMP-28 was highest in healthy tissues when compared to diseased periodontal tissues suggesting that MMP-28 could act as a biomarker for periodontal health.
authors.affiliation: Department of Periodontology, Rajarajeswari Dental College and Hospital, Bangalore, Karnataka 560085, India.
authors.forename: P
authors.initials: P
authors.lastname: Padmavati
authors.forename: S
authors.initials: S
authors.lastname: Savita
authors.forename: B M
authors.initials: BM
authors.lastname: Shivaprasad
authors.forename: Krishna
authors.initials: K
authors.lastname: Kripal
authors.forename: K
authors.initials: K
authors.lastname: Rithesh
mesh_terms: D000293:Adolescent; D000328:Adult; D010520:Aggressive Periodontitis / Q000201:enzymology*; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000201:enzymology*; D003430:Cross-Sectional Studies; D005260:Female; D005881:Gingiva / Q000201:enzymology*; D006801:Humans; D008297:Male; D053505:Matrix Metalloproteinases, Secreted / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; C424663:MMP28 protein, human; D053505:Matrix Metalloproteinases, Secreted