title: Interactions of doxorubicin with organized interfacial assemblies. 2. Spectroscopic characterization.
abstract: Doxorubicin is an anthracycline that has found wide use as a chemotherapeutic agent, with the primary limitation to its use being cardiotoxicity. Depending on the identity and location of pendent side groups, the anthracyclines exhibit varying degrees of chemotherapeutic activity and toxicity, and a key area of research activity lies in understanding how the structure of the anthracycline influences its interactions with amphiphilic interfaces. We have studied interactions between doxorubicin and interfacial adlayers of octadecylamine (C18NH2), dihexadecylphosphate (DHP), and both monolayers and bilayers of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) on mica using time- and frequency-resolved spectroscopic measurements. We report surface-enhanced resonance Raman data and fluorescence lifetime and anisotropy imaging data for doxorubicin at these interfaces. For all monolayers, there is a substantial interaction between doxorubicin and the interface. For DMPC bilayers, the extent of the interaction between doxorubicin and the interface depends on how the interface was formed.
authors.affiliation: Faculty of Chemistry, University of Warsaw , 02-093 Warsaw, Pasteur 1, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Nieciecka
authors.forename: Agata
authors.initials: A
authors.lastname: Królikowska
authors.forename: Iwan
authors.initials: I
authors.lastname: Setiawan
authors.forename: Pawel
authors.initials: P
authors.lastname: Krysinski
authors.forename: G J
authors.initials: GJ
authors.lastname: Blanchard
mesh_terms: D000588:Amines / Q000737:chemistry*; D016880:Anisotropy; D000903:Antibiotics, Antineoplastic / Q000737:chemistry*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D004317:Doxorubicin / Q000737:chemistry*; D008051:Lipid Bilayers / Q000138:chemical synthesis / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D008968:Molecular Conformation; D010755:Organophosphates / Q000737:chemistry*; D013059:Spectrum Analysis, Raman; D013499:Surface Properties; D013997:Time Factors
chemical_list: D000588:Amines; D000903:Antibiotics, Antineoplastic; D008051:Lipid Bilayers; D010755:Organophosphates; D004317:Doxorubicin; C009317:stearylamine; D004134:Dimyristoylphosphatidylcholine