title: Insulin-like growth factor I (IGF-1) Ec/Mechano Growth factor--a splice variant of IGF-1 within the growth plate.
abstract: Human insulin-like growth factor 1 Ec (IGF-1Ec), also called mechano growth factor (MGF), is a splice variant of insulin-like growth factor 1 (IGF-1), which has been shown in vitro as well as in vivo to induce growth and hypertrophy in mechanically stimulated or damaged muscle. Growth, hypertrophy and responses to mechanical stimulation are important reactions of cartilaginous tissues, especially those in growth plates. Therefore, we wanted to ascertain if MGF is expressed in growth plate cartilage and if it influences proliferation of chondrocytes, as it does in musculoskeletal tissues. MGF expression was analyzed in growth plate and control tissue samples from piglets aged 3 to 6 weeks. Furthermore, growth plate chondrocyte cell culture was used to evaluate the effects of the MGF peptide on proliferation. We showed that MGF is expressed in considerable amounts in the tissues evaluated. We found the MGF peptide to be primarily located in the cytoplasm, and in some instances, it was also found in the nucleus of the cells. Addition of MGF peptides was not associated with growth plate chondrocyte proliferation.
authors.affiliation: Medical University of Vienna, Department of Paediatrics and Adolescent Medicine, Vienna, Austria.
authors.forename: Werner
authors.initials: W
authors.lastname: Schlegel
authors.forename: Adalbert
authors.initials: A
authors.lastname: Raimann
authors.forename: Daniel
authors.initials: D
authors.lastname: Halbauer
authors.forename: Daniela
authors.initials: D
authors.lastname: Scharmer
authors.forename: Susanne
authors.initials: S
authors.lastname: Sagmeister
authors.forename: Barbara
authors.initials: B
authors.lastname: Wessner
authors.forename: Magdalena
authors.initials: M
authors.lastname: Helmreich
authors.forename: Gabriele
authors.initials: G
authors.lastname: Haeusler
authors.forename: Monika
authors.initials: M
authors.lastname: Egerbacher
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D015870:Gene Expression; D006132:Growth Plate / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics* / Q000378:metabolism; D040542:Mechanotransduction, Cellular; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism; D010455:Peptides / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D061251:Primary Cell Culture; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013552:Swine
chemical_list: D010455:Peptides; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I