title: C70-carboxyfullerenes as efficient antioxidants to protect cells against oxidative-induced stress.
abstract: Oxidative stress induced by excessive production of reactive oxygen species (ROS) has been implicated in the etiology of many human diseases. Acquiring a highly efficient antioxidant with good biocompatibility is of significance in eliminating the deleterious effect induced by the oxidative stress. Herein, we address our efforts on investigating the cytoprotective effect of carboxyfullerenes on H2O2-injured cells. Meanwhile, the uptake and intracellular location of carboxyfullerenes were studied. The results show that C70-carboxyfullerenes (dimalonic acid C70 fullerene (DF70) and trimalonic acid C70 fullerene (TF70)) exhibit an obviously protective effect against oxidative stress on C2C12 cells at concentrations as low as 2.5 μmol L(-1), whereas C60-carboxyfullerenes (dimalonic acid C60 fullerene (DF60) and quadri-malonic acid C60 fullerene (QF60)) show a protective effect at relatively higher concentration (40 μmol L(-1)). The molecular structure of carboxyfullerenes and the physiological state of cells play an important role in the different cytoprotective capability. Further study reveals that DF70 and TF70 could enter into cells and mainly localize into the lysosome, which possibly involves the protective mechanism by stabilizing lysosome. The use of a significantly low concentration of C70-carboxyfullerene as the antioxidative agent will benefit the therapeutic approaches aiming at alleviating ROS-induced injuries such as muscle disorder and arthritis.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Liu
authors.forename: Xuejie
authors.initials: X
authors.lastname: Zhang
authors.forename: Xinyue
authors.initials: X
authors.lastname: Zhang
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhang
authors.forename: Junpeng
authors.initials: J
authors.lastname: Zheng
authors.forename: Mirong
authors.initials: M
authors.lastname: Guan
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Fang
authors.forename: Chunru
authors.initials: C
authors.lastname: Wang
authors.forename: Chunying
authors.initials: C
authors.lastname: Shu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002264:Carboxylic Acids / Q000737:chemistry; D002460:Cell Line; D037741:Fullerenes / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; C416673:C60 carboxyfullerene; D002264:Carboxylic Acids; D037741:Fullerenes; D020011:Protective Agents; D017382:Reactive Oxygen Species; C069836:fullerene C70; D006861:Hydrogen Peroxide