title: Structural investigation of a novel heteropolysaccharide from the fruiting bodies of Boletus edulis.
abstract: A novel water-soluble heteropolysaccharide, BEPF1, was isolated from the fruiting bodies of Boletus edulis with boiling water extraction and purified by Sephacryl S-300, with a molecular weight of 1.08×10(4)Da. Sugar composition of BEPF1 showed that it was composed of l-fucose, d-mannose, d-glucose and d-galactose in the ratio of 0.21:0.23:1.17:1.00. Methylation analysis together with (1)H, (13)C and 2D NMR spectroscopy established that BEPF1 was consisted of α-d-(1→6)-galactopyranan backbone with a terminal of α-l-fucosyl unit on O-2 of the 2-d-(2→6)-galactosyl units, β-d-(1→6)-4-O-Me-glucopyranan and β-d-(1→6)-glucopyranan backbone with a terminal β-d-glucosyl unit and it also contained a minor of 2,6-β-d-Mannopyranan residues.
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
authors.forename: An-qiang
authors.initials: AQ
authors.lastname: Zhang
authors.forename: Ye
authors.initials: Y
authors.lastname: Liu
authors.forename: Nan-nan
authors.initials: NN
authors.lastname: Xiao
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Pei-long
authors.initials: PL
authors.lastname: Sun
mesh_terms: D001487:Basidiomycota / Q000737:chemistry* / Q000378:metabolism; D002240:Carbohydrate Sequence; D048690:Fruiting Bodies, Fungal / Q000737:chemistry* / Q000378:metabolism; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight
chemical_list: D062610:Fungal Polysaccharides
keywords: Boletus edulis; Heteropolysaccharide; NMR analysis; Structural elucidation