title: Anti HSV-1 activity of halistanol sulfate and halistanol sulfate C isolated from Brazilian marine sponge Petromica citrina (Demospongiae).
abstract: The n-butanol fraction (BF) obtained from the crude extract of the marine sponge Petromica citrina, the halistanol-enriched fraction (TSH fraction), and the isolated compounds halistanol sulfate (1) and halistanol sulfate C (2), were evaluated for their inhibitory effects on the replication of the Herpes Simplex Virus type 1 (HSV-1, KOS strain) by the viral plaque number reduction assay. The TSH fraction was the most effective against HSV-1 replication (SI = 15.33), whereas compounds 1 (SI = 2.46) and 2 (SI = 1.95) were less active. The most active fraction and these compounds were also assayed to determine the viral multiplication step(s) upon which they act as well as their potential synergistic effects. The anti-HSV-1 activity detected was mediated by the inhibition of virus attachment and by the penetration into Vero cells, the virucidal effect on virus particles, and by the impairment in levels of ICP27 and gD proteins of HSV-1. In summary, these results suggest that the anti-HSV-1 activity of TSH fraction detected is possibly related to the synergic effects of compounds 1 and 2.
authors.affiliation: Laboratory of Natural Products, Department of Pharmaceutical Science, Universidade Federal de Santa Catarina, Florianópolis 88040-900, SC, Brazil. claudiasimoes13@gmail.com.
authors.forename: Tatiana
authors.initials: T
authors.lastname: da Rosa Guimarães
authors.forename: Carlos Guillermo
authors.initials: CG
authors.lastname: Quiroz
authors.forename: Caroline Rigotto
authors.initials: CR
authors.lastname: Borges
authors.forename: Simone Quintana
authors.initials: SQ
authors.lastname: de Oliveira
authors.forename: Maria Tereza Rojo
authors.initials: MT
authors.lastname: de Almeida
authors.forename: Éverson Miguel
authors.initials: ÉM
authors.lastname: Bianco
authors.forename: Maria Izabel Goulart
authors.initials: MI
authors.lastname: Moritz
authors.forename: João Luís
authors.initials: JL
authors.lastname: Carraro
authors.forename: Jorge Alejandro
authors.initials: JA
authors.lastname: Palermo
authors.forename: Gabriela
authors.initials: G
authors.lastname: Cabrera
authors.forename: Eloir Paulo
authors.initials: EP
authors.lastname: Schenkel
authors.forename: Flávio Henrique
authors.initials: FH
authors.lastname: Reginatto
authors.forename: Cláudia Maria Oliveira
authors.initials: CM
authors.lastname: Simões
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry* / Q000494:pharmacology*; D001938:Brazil; D002460:Cell Line; D002522:Chlorocebus aethiops; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D011161:Porifera / Q000737:chemistry*; D013261:Sterols / Q000737:chemistry* / Q000494:pharmacology*; D014709:Vero Cells; D010948:Viral Plaque Assay / Q000379:methods
chemical_list: D000998:Antiviral Agents; D013261:Sterols; C072244:halistanol trisulfate; D020001:1-Butanol