title: Comparison of wet-mount, Wright-Giemsa and Gram-stained urine sediment for predicting bacteriuria in dogs and cats.
abstract: This study assessed the standard urinalysis technique and sediment stain techniques as predictors of bacterial culture results for canine and feline urine. Canine (n = 111) and feline (n = 79) urine samples were evaluated using unstained wet-mount and air-dried Gram and Wright-Giemsa stained sediment; results were compared to aerobic bacterial culture. Eleven canine and 7 feline urine samples were culture positive. Unstained wet-mount and stained sediment had sensitivities of 89% and 83% and specificities of 91% and 99%, respectively. The specificity of using either stain was higher (P < 0.01) than wet-mount examination for detecting bacteriuria. There were significant differences among 3 technologists in detecting true positives (P < 0.01). Association of sediment and culture results used 112 canine and 81 feline samples. There was a negative association (P < 0.01) between lipid detection and wet-mount identification of bacteria.
authors.affiliation: Department of Pathology and Microbiology (O'Neil, Horney, Burton, Lewis, MacKenzie), Department of Health Management, (Stryhn), Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: O'Neil
authors.forename: Barbara
authors.initials: B
authors.lastname: Horney
authors.forename: Shelley
authors.initials: S
authors.lastname: Burton
authors.forename: P Jeffrey
authors.initials: PJ
authors.lastname: Lewis
authors.forename: Allan
authors.initials: A
authors.lastname: MacKenzie
authors.forename: Henrik
authors.initials: H
authors.lastname: Stryhn
mesh_terms: D000818:Animals; D001437:Bacteriuria / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D002371:Cat Diseases / Q000175:diagnosis* / Q000382:microbiology; D002415:Cats; D004283:Dog Diseases / Q000175:diagnosis* / Q000382:microbiology; D004285:Dogs; D005260:Female; D008297:Male; D012680:Sensitivity and Specificity; D016482:Urinalysis / Q000379:methods*; D014552:Urinary Tract Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*