title: Validation of the boronate sensor ContPY1 as a specific probe for fluorescent detection of hydrogen peroxide in plants.
abstract: Studying the implication of hydrogen peroxide in biological processes in plants remains a challenge due to the current shortcomings of H2O2-responsive probes. The use of ContPY1, a new fluorescent probe, which is highly selective and sensitive for H2O2, was investigated. To validate the use of ContPY1 on plants, we have generated protocols employing cells suspensions and leaves, and measured specifically H2O2 production by plants using spectrofluorometry and microscopy.
authors.affiliation: Département Valorisation des Productions Agricoles; Centre Wallon de Recherches Agronomiques; Gembloux, Belgium; Laboratoire de Chimie Organique et Médicinale; Université catholique de Louvain; Louvain-la-Neuve, Belgium.
authors.forename: Quentin
authors.initials: Q
authors.lastname: Ledoux
authors.affiliation: Département Valorisation des productions agricoles; Centre Wallon de Recherches Agronomiques; Gembloux, Belgium.
authors.forename: Pascal
authors.initials: P
authors.lastname: Veys
authors.affiliation: Unité de Recherche en Biologie Cellulaire Végétale; Université de Namur; Namur, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Van Cutsem
authors.affiliation: Département Sciences du Vivant; Centre Wallon de Recherches Agronomiques; Gembloux, Belgium.
authors.forename: Sergio
authors.initials: S
authors.lastname: Mauro
authors.affiliation: Laboratoire de Chimie Organique et Médicinale; Université catholique de Louvain; Louvain-la-Neuve, Belgium.
authors.forename: Fabio
authors.initials: F
authors.lastname: Lucaccioni
authors.affiliation: Laboratoire de Chimie Organique et Médicinale; Université catholique de Louvain; Louvain-la-Neuve, Belgium.
authors.forename: Istvan E
authors.initials: IE
authors.lastname: Marko
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D001896:Boron Compounds / Q000378:metabolism*; D001897:Boronic Acids / Q000378:metabolism*; D005452:Fluoresceins / Q000378:metabolism; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D015335:Molecular Probes / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism; D059008:Time-Lapse Imaging
chemical_list: D001896:Boron Compounds; D001897:Boronic Acids; C000593474:ContPY1; D005452:Fluoresceins; D005456:Fluorescent Dyes; D015335:Molecular Probes; C029569:diacetyldichlorofluorescein; D006861:Hydrogen Peroxide
keywords: Arabidopsis; ContPY1; cells suspension; fluorescence; hydrogen peroxide; microscopy; protoplasts