title: Two isoforms of acid phosphatase secreted by tobacco protoplasts: differential effect of brefeldin A on their secretion.
abstract: The effects of brefeldin A (BFA) on the secretion of acid phosphatase (APase) by tobacco protoplasts were investigated. Secretion of APase was inhibited by BFA in a dose-dependent manner, with a concomitant intracellular accumulation of the enzyme. The secreted APase was composed of two isoforms. BFA (10/ μg/ml) inhibited the secretion of one of the isoforms without inhibiting that of the other, and this phenomenon explains the partial inhibition of APase secretion as a whole. The inhibition of APase secretion was accompanied by changes in the morphology of the Golgi apparatus and also by an increment in massdensity of cells.
authors.affiliation: Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Mejiro-dai, Bunkyo-ku, 112, Tokyo, Japan.
authors.forename: T S
authors.initials: TS
authors.lastname: Kaneko
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Osumi
authors.forename: M
authors.initials: M
authors.lastname: Muroi
authors.forename: A
authors.initials: A
authors.lastname: Takatsuki