title: Shoot regeneration in long-term callus cultures derived from mature flowering plants of Cyclamen persicum Mill.
abstract: In long-term callus cultures of Cyclamen persicum Mill. two types of tissue could be distinguished. One type featured a brown suberised outer layer and was poorly organogenic. The other type was yellowish in appearance and gave rise to many shoot buds. Both types co-existed on the same callus, the former prevailing. Selection for organogenic tissue resulted in cultures yielding approximately three times more petioles than random subcultures. Callus-derived shoots could be rooted and established in the greenhouse. The method allowed for the production of thousands of plants but the regenerants often showed deviant phenotypes and genotypes.
authors.affiliation: S&G Seeds B.V., Westeinde 62/P.O.Box 26, NL-1600, AA Enkhuizen, The Netherlands.
authors.forename: W
authors.initials: W
authors.lastname: Dillen
authors.forename: I
authors.initials: I
authors.lastname: Dijkstra
authors.forename: J
authors.initials: J
authors.lastname: Oud