title: S100A7 in the Fallopian tube: a comparative study.
abstract: The oviduct is a dynamic organ in which final gamete maturation, fertilization and early embryo development take place. It is considered to be a sterile site; however the mechanism for sterility maintenance is still unknown. S100A7 is an anti-microbial peptide that has been reported in human reproductive tissues such as prostate, testicle, ovary, normal cervical epithelium and sperm. The current work reports the presence of S100A7 in the Fallopian tube and its localization at the apical surface of epithelial cells. For comparison, porcine S100A7 was used for antibody development and search for peptide in reproductive tissues. Although present in boar seminal vesicles and seminal plasma, S100A7 was not detected on female porcine organs. Also, in contrast with the human protein, porcine S100A7 did not show anti-microbial activity under the conditions tested. Phylogenetic analyses showed high divergence of porcine S100A7 from human, primate, bovine, ovine and equine sequences, being the murine sequence at a most distant branch. The differences in sequence homology, Escherichia coli-cidal activity, detectable presence and localization of S100A7 from human and pig, suggest that there are possible different functions in each organism.
authors.affiliation: Área Biología,Facultad de Ciencias Bioquímicas y Farmacéuticas,Universidad Nacional de Rosario,Suipacha 590,Rosario S2002LRK,Argentina.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Teijeiro
authors.affiliation: Facultad de Ciencias Bioquímicas y Farmacéuticas,Universidad Nacional de Rosario,Suipacha 590,Rosario S2002LRK,Argentina.
authors.forename: Patricia E
authors.initials: PE
authors.lastname: Marini
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002417:Cattle; D004847:Epithelial Cells / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects; D005187:Fallopian Tubes / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006736:Horses; D008297:Male; D051379:Mice; D010802:Phylogeny; D011323:Primates; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D012661:Semen / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D034421:Sus scrofa
chemical_list: D000900:Anti-Bacterial Agents; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins; C089242:S100A7 protein, human; C506174:S100a7a protein, mouse
keywords: Seminal plasma