title: Acute toxic effects of 3,3'-iminodipropionitrile on hypothalamic-pituitary-gonadal axis in male rats.
abstract: Exposure to 3,3'-iminodipropionitrile (IDPN) causes persistent neurotoxicity, while its reproductive toxicity in female rats is transient, indicating that gonadotropin-releasing hormone (GnRH) neurons and gonadotrophs receive little or no damage from IDPN and that the transient gonadal toxicity may be also observed in males. To clarify these points, the acute toxic effects of IDPN on hypothalamic-pituitary-gonadal axis of male rats were examined histologically, biochemically and serologically. A single intraperitoneal injection of IDPN (1000 mg/kg body weight) induced signs of neurotoxicity within a day; nevertheless, GnRH neurons were not affected throughout the experimental period. Four days after IDPN treatment, the plasma level of testosterone but not gonadotropins decreased and active caspase 3-immunopositive spermatids increased; both parameters returned to normal levels afterwards. Data from our studies revealed that while IDPN had little or no toxic effect on GnRH neurons or gonadotrophs it was transiently toxic to gonads in both sexes.
authors.affiliation: Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Sugao 2-16-1, Miyamae-ku, Kawasaki, Kanagawa 216-8511, Japan. Electronic address: n-tkhs@marianna-u.ac.jp.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Takahashi
authors.affiliation: Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Sugao 2-16-1, Miyamae-ku, Kawasaki, Kanagawa 216-8511, Japan. Electronic address: naomi@marianna-u.ac.jp.
authors.forename: Naomi
authors.initials: N
authors.lastname: Hamada
authors.affiliation: Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Sugao 2-16-1, Miyamae-ku, Kawasaki, Kanagawa 216-8511, Japan. Electronic address: ishizuka@marianna-u.ac.jp.
authors.forename: Bunpei
authors.initials: B
authors.lastname: Ishizuka
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects / Q000473:pathology; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism; D006400:Hematocrit; D007030:Hypothalamo-Hypophyseal System / Q000166:cytology / Q000187:drug effects*; D008297:Male; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D009498:Neurotoxins / Q000633:toxicity*; D009570:Nitriles / Q000633:toxicity*; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000187:drug effects*
chemical_list: D009498:Neurotoxins; D009570:Nitriles; D007987:Gonadotropin-Releasing Hormone; C008110:3,3'-iminodipropionitrile; D053148:Caspase 3
keywords: 3,3′-Iminodipropionitrile; Anterior pituitary; GnRH neuron; Reproductive toxicity; Testis