title: Development of a mucin4-targeting SPIO contrast agent for effective detection of pancreatic tumor cells in vitro and in vivo.
abstract: In search of a unique and reliable contrast agent targeting pancreatic adenocarcinoma, new multifunctional nanoparticles (MnMEIO-silane-NH2-(MUC4)-mPEG NPs) were successfully developed in this study. Mucin4-expression levels were determined through different imaging studies in a panel of pancreatic tumor cells (HPAC, BxPC-3, and Panc-1) both in vitro and in vivo studies. The in vitro T2-weighted MR imaging study in HPAC and Panc-1 tumor cells treated with NPs showed -89.1 ± 5.7% and -0.9 ± 0.2% contrast enhancement, whereas in in vivo study, it is found to be -81.5 ± 4.5% versus -19.6 ± 5.2% (24 h postinjection, 7.0 T), respectively. The T2-weighted MR and optical imaging studies revealed that the novel contrast agent can specifically and effectively target to mucin4-expressing tumors in nude mice. Hence, it is suggested that MnMEIO-silane-NH2-(MUC4)-mPEG NPs are able to provide an efficient and targeted delivery of MUC4 antibodies to mucin4-expressing pancreatic tumors.
authors.affiliation: Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, National Chiao Tung University , 75 Bo-Ai Street, Hsinchu 300, Taiwan.
authors.forename: Shou-Cheng
authors.initials: SC
authors.lastname: Wu
authors.forename: Yu-Jen
authors.initials: YJ
authors.lastname: Chen
authors.forename: Yi-Jan
authors.initials: YJ
authors.lastname: Lin
authors.forename: Tung-Ho
authors.initials: TH
authors.lastname: Wu
authors.forename: Yun-Ming
authors.initials: YM
authors.lastname: Wang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics; D055808:Drug Discovery; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry* / Q000378:metabolism / Q000493:pharmacokinetics; D006801:Humans; D008279:Magnetic Resonance Imaging; D059346:Magnets; D051379:Mice; D055264:Mucin-4 / Q000378:metabolism*; D053758:Nanoparticles; D061848:Optical Imaging; D010190:Pancreatic Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D011092:Polyethylene Glycols / Q000737:chemistry; D012821:Silanes / Q000737:chemistry
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; D055264:Mucin-4; D012821:Silanes; C000499:ferric oxide; D011092:Polyethylene Glycols