title: Identification of a set of RFLP probes for subspecies differentiation in Oryza sativa L.
abstract: Sixty-eight indica-japonica tester-differentiating RFLP probes were tested in seven indica and seven japonica varieties of rice (Oryza sativa L.) with four enzyme digestions (EcoRI, EcoRV, HindIII and DraI). Twenty-one DNA clones were isolated as indica-japonica subspecies-differentiating probes. A set of 13 probes was established as core probes for subspecies differentiation and a pooled blotting analysis was carried out to facilitate the application of RFLP in rice genetics and breeding practice. A dendrogram of 12 wide-compatibility varieties was constructed based on RFLPs detected by 13 core probes with single enzyme digestions. It was speculated that most RFLPs of indica-japonica differentiating probes were generated by insertions/deletions, which may be of great significance for the origin and differentiation of subspecies in Oryza sativa L.
authors.affiliation: Biotechnology Department, China National Rice Research Institute, 310006, Hangzhou, P. R. China.
authors.forename: H R
authors.initials: HR
authors.lastname: Qian
authors.forename: J Y
authors.initials: JY
authors.lastname: Zhuang
authors.forename: H X
authors.initials: HX
authors.lastname: Lin
authors.forename: J
authors.initials: J
authors.lastname: Lu
authors.forename: K L
authors.initials: KL
authors.lastname: Zheng