title: Bioactive polyhydroxylated sterols from the marine sponge Haliclona crassiloba.
abstract: Four new polyhydroxylated sterols, named halicrasterols A-D (1-4), together with six known analogs (5-10) were isolated from the marine sponge Haliclona crassiloba. Compounds 1 and 2 represented rare examples of steroids featuring 17(20)E-double bonds. The structures of 1-10 were elucidated by spectroscopic analysis and comparison with reported data. This is the first report of a steroid profile for this species. The antimicrobial activities of 1-10 were evaluated against a panel of bacterial and fungal strains in vitro, and compounds 4 and 9 showed moderate activity against some of the Gram-positive strains with MICs ranging from 4 to 32 μg/mL.
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, PR China.
authors.forename: Zhong-Bin
authors.initials: ZB
authors.lastname: Cheng
authors.forename: Han
authors.initials: H
authors.lastname: Xiao
authors.forename: Cheng-Qi
authors.initials: CQ
authors.lastname: Fan
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Lu
authors.forename: Ge
authors.initials: G
authors.lastname: Zhang
authors.forename: Sheng
authors.initials: S
authors.lastname: Yin
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001407:Bacillus / Q000187:drug effects / Q000254:growth & development; D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development; D002175:Candida / Q000187:drug effects / Q000254:growth & development; D013293:Enterococcus faecalis / Q000187:drug effects / Q000254:growth & development; D047129:Haliclona / Q000737:chemistry* / Q000378:metabolism; D006900:Hydroxylation; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013261:Sterols / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D013261:Sterols
keywords: Antibacterial activity; Haliclona crassiloba; Polyhydroxylated sterols; Structure elucidation