title: Screening effective short interfering RNA/short hairpin RNA for inhibition of human astrovirus ORF2 gene expression in cultured cells.
abstract: In this study, we have evaluated four different 21-nt duplexes of small interfering RNA (siRNA-469, siRNA-852, siRNA-1802 and siRNA-1806) that specifically target the ORF2 gene of human astrovirus (HAstV) in inhibiting HAstV capsid protein expression in transfected BHK-21 cells. Furthermore, fluorescence analysis, real-time quantitative PCR (RT-qPCR) and western blot assays showed that pGPU6/GFP/Neo-shRNA inhibits ORF2 gene expression in Caco2 cells. The results indicate that siRNA/shRNA-469 and siRNA/shRNA-1802 can interfere with capsid protein expression in cell culture, and this provides a powerful tool for the study of HAstV gene functions and the biological properties of the capsid protein.
authors.affiliation: School of Basic Medical Sciences, Liaoning Medical University, No. 40, The Third Section of SongPo Rd, Jinzhou, 121200, Liaoning, People's Republic of China, zhaowei8072@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Ke
authors.initials: K
authors.lastname: Niu
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Jin
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Sui
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D006801:Humans; D001257:Mamastrovirus / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014764:Viral Proteins