title: Upregulation of CREM-1 relates to retinal ganglion cells apoptosis after light-induced damage in vivo.
abstract: Previous studies have shown activation of cyclic AMP response element-binding protein (CREB) family is involved in the retinal ganglion cells (RGCs) protection. However, the function of cyclic AMP response element modulator-1 (CREM-1), one member of the CREB family, is still with limited acquaintance. To investigate whether CREM-1 is involved in RGCs death, we performed a light-induced retinal damage model in adult rats. Upregulation of CREM-1 was observed in retina after light-induced damage by performing western blot. Immunofluorescent labeling indicated that upregulated CREM-1 was localized mainly in the RGCs. We also investigated co-localization of CREM-1 with active-caspase-3 and TUNEL (apoptotic markers) in the retina after light-induced damage. In addition, the expression patterns of B cell lymphoma/leukemia-2 and Bcl-2 associated X protein were parallel with that of CREM-1. Collectively, we hypothesized upregulation of CREM-1 in the retina was associated with RGCs death after light-induced damage.
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong Province, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Xu
authors.forename: Shanshan
authors.initials: S
authors.lastname: Yu
authors.forename: Qinmeng
authors.initials: Q
authors.lastname: Shu
authors.forename: Lu
authors.initials: L
authors.lastname: Yang
authors.forename: Cheng
authors.initials: C
authors.lastname: Yang
authors.forename: Jiawei
authors.initials: J
authors.lastname: Wang
authors.forename: Fan
authors.initials: F
authors.lastname: Xu
authors.forename: Min
authors.initials: M
authors.lastname: Ji
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Liang
mesh_terms: D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D051786:Cyclic AMP Response Element Modulator / Q000235:genetics / Q000378:metabolism*; D008027:Light / Q000009:adverse effects; D008297:Male; D011833:Radiation Injuries, Experimental / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012165:Retinal Ganglion Cells / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects; D015854:Up-Regulation
chemical_list: D051017:Apoptosis Regulatory Proteins; C497412:Crem protein, rat; D051786:Cyclic AMP Response Element Modulator