title: Derivation and network formation of vascular cells from human pluripotent stem cells.
abstract: As the lifeline of almost all living tissues, blood vessels are a major focus of tissue-regenerative therapies. Rebuilding blood vessels has vast implications for the study of vascular growth and treatment of diseases in which vascular function is compromised. Toward this end, human pluripotent stem cells have been widely studied for their differentiation capacity toward vascular lineages. We demonstrate methods to derive a bicellular population of early specialized vascular cells from human pluripotent stem cells, to differentiate these toward mature endothelial cells and pericytes, and to utilize a collagen scaffold to facilitate organization into vascular networks.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences, and Oncology Center and Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA.
authors.forename: Sravanti
authors.initials: S
authors.lastname: Kusuma
authors.forename: Bria
authors.initials: B
authors.lastname: Macklin
authors.forename: Sharon
authors.initials: S
authors.lastname: Gerecht
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000166:cytology* / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D005782:Gels / Q000494:pharmacology; D006801:Humans; D018919:Neovascularization, Physiologic / Q000187:drug effects; D020286:Pericytes / Q000166:cytology / Q000187:drug effects; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects; D051381:Rats
chemical_list: D005782:Gels; D003094:Collagen