title: Small RNA library construction for high-throughput sequencing.
abstract: Since their discovery about 20 years ago, small RNAs have been shown to play a critical role in a myriad of biological processes. The greater availability of high-throughput sequencing has been invaluable to furthering our understanding of small RNAs as regulatory molecules. In particular, these sequencing technologies have been crucial in understanding the role of small RNAs in reproductive tissues, where millions of individual sequences are generated. In this context, high-throughput sequencing provides the requisite level of resolution that other procedures, like northern blotting, would not be able to achieve. Here, we describe a protocol for the preparation of small RNA libraries for sequencing using the Solexa/Illumina technology.
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.
authors.forename: Jon
authors.initials: J
authors.lastname: McGinn
authors.forename: Benjamin
authors.initials: B
authors.lastname: Czech
mesh_terms: D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D016133:Polymerase Chain Reaction; D058727:RNA, Small Untranslated / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D048348:Reverse Transcription; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D018076:DNA, Complementary; D058727:RNA, Small Untranslated; C078388:endodeoxyribonuclease PmeI; D015252:Deoxyribonucleases, Type II Site-Specific