title: High-expression of DJ-1 and loss of PTEN associated with tumor metastasis and correlated with poor prognosis of gastric carcinoma.
abstract: OBJECTIVE
  DJ-1 and PTEN have been shown to involve in multiple cell processes and play an important role in cancer development and progression. However, their relationship with gastric carcinoma (GC) has not been identified yet. The purpose of this study is to clarify the relationship of DJ-1 and phosphatase and tensin homolog (PTEN) with clinicopathological parameters and prognosis in GC.
  
  
  METHODS
  114 specimens were collected from GC patients and expression of DJ-1 and PTEN in tissue microarray was evaluated by immunohistochemical staining. Correlation between immunostainings and clinicopathological parameters, follow-up data of patients, was analyzed statistically.
  
  
  RESULTS
  High expression of DJ-1 was found in 66.7% (76/114) and associated with tumor depth (P=0.003), lymph node metastasis (P=0.011), distant metastasis (P=0.001) and advanced clinical stage (P=0.001). Loss or downregulation of PTEN was found in 58.7% (67/114) and associated with advanced clinical stage (P=0.018) and high expression of DJ-1 in tumor cells (P=0.006). In univariate survival analysis, high-expression of DJ-1 or loss of PTEN was significantly associated with poor prognosis of GC patients. However, only tumor depth (P=0.011) and coexistence of DJ-1 and PTEN abnormal expression (P=0.009) emerged as strong independent prognostic factors for overall survival of GC patients.
  
  
  CONCLUSIONS
  the present study indicates that DJ-1 and PTEN may play their roles in progression of GC in a cooperating pattern. Co-existence of abnormal DJ-1 and PTEN expression is likely to serve as an independent predictive factor for prognosis of GC patients.
authors.affiliation: 1. Department of Gastrointestinal Surgery, The First Affiliated Hospital, Sun Yat-sen University, 58, Zhongshan Road II, Guangzhou 510080, China;
authors.forename: Yin
authors.initials: Y
authors.lastname: Li
authors.forename: Ji
authors.initials: J
authors.lastname: Cui
authors.forename: Chang-hua
authors.initials: CH
authors.lastname: Zhang
authors.forename: Dong-jie
authors.initials: DJ
authors.lastname: Yang
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Chen
authors.forename: Wen-hua
authors.initials: WH
authors.lastname: Zan
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.forename: Yu-long
authors.initials: YL
authors.lastname: He
mesh_terms: D000368:Aged; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000235:genetics / Q000473:pathology; D015513:Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D051059:PTEN Phosphohydrolase / Q000096:biosynthesis* / Q000235:genetics; D016612:Paraffin Embedding; D011379:Prognosis; D016016:Proportional Hazards Models; D000071617:Protein Deglycase DJ-1; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: DJ-1; Gastric carcinoma; Metastasis; PTEN; Prognosis.