title: Biomimetic mineralization on a macroporous cellulose-based matrix for bone regeneration.
abstract: The aim of this study is to investigate the biomimetic mineralization on a cellulose-based porous matrix with an improved biological profile. The cellulose matrix was precalcified using three methods: (i) cellulose samples were treated with a solution of calcium chloride and diammonium hydrogen phosphate; (ii) the carboxymethylated cellulose matrix was stored in a saturated calcium hydroxide solution; (iii) the cellulose matrix was mixed with a calcium silicate solution in order to introduce silanol groups and to combine them with calcium ions. All the methods resulted in a mineralization of the cellulose surfaces after immersion in a simulated body fluid solution. Over a period of 14 days, the matrix was completely covered with hydroxyapatite crystals. Hydroxyapatite formation depended on functional groups on the matrix surface as well as on the precalcification method. The largest hydroxyapatite crystals were obtained on the carboxymethylated cellulose matrix treated with calcium hydroxide solution. The porous cellulose matrix was not cytotoxic, allowing the adhesion and proliferation of human osteoblastic cells. Comparatively, improved cell adhesion and growth rate were achieved on the mineralized cellulose matrices.
authors.affiliation: Department of Organic Technology, Kaunas University of Technology, Radvilenu pl. 19, 50254 Kaunas, Lithuania.
authors.forename: Odeta
authors.initials: O
authors.lastname: Petrauskaite
authors.forename: Pedro de Sousa
authors.initials: Pde S
authors.lastname: Gomes
authors.forename: Maria Helena
authors.initials: MH
authors.lastname: Fernandes
authors.forename: Gintaras
authors.initials: G
authors.lastname: Juodzbalys
authors.forename: Arturas
authors.initials: A
authors.lastname: Stumbras
authors.forename: Julius
authors.initials: J
authors.lastname: Maminskas
authors.forename: Jolanta
authors.initials: J
authors.lastname: Liesiene
authors.forename: Marco
authors.initials: M
authors.lastname: Cicciù
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D002113:Calcification, Physiologic / Q000187:drug effects; D002122:Calcium Chloride / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D017886:Durapatite / Q000737:chemistry / Q000627:therapeutic use; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010710:Phosphates / Q000494:pharmacology; D013499:Surface Properties
chemical_list: D010710:Phosphates; C024788:ammonium phosphate; D002482:Cellulose; D017886:Durapatite; D002122:Calcium Chloride