title: MicroRNA profiling in prostate cancer--the diagnostic potential of urinary miR-205 and miR-214.
abstract: Prostate cancer (PCa) is the most common type of cancer in men in the United States, which disproportionately affects African American descents. While metastasis is the most common cause of death among PCa patients, no specific markers have been assigned to severity and ethnic biasness of the disease. MicroRNAs represent a promising new class of biomarkers owing to their inherent stability and resilience. In the present study, we investigated potential miRNAs that can be used as biomarkers and/or therapeutic targets and can provide insight into the severity and ethnic biasness of PCa. PCR array was performed in FFPE PCa tissues (5 Caucasian American and 5 African American) and selected differentially expressed miRNAs were validated by qRT-PCR, in 40 (15 CA and 25 AA) paired PCa and adjacent normal tissues. Significantly deregulated miRNAs were also analyzed in urine samples to explore their potential as non-invasive biomarker for PCa. Out of 8 miRNAs selected for validation from PCR array data, miR-205 (p<0.0001), mir-214 (p<0.0001), miR-221(p<0.001) and miR-99b (p<0.0001) were significantly downregulated in PCa tissues. ROC curve shows that all four miRNAs successfully discriminated between PCa and adjacent normal tissues. MiR-99b showed significant down regulation (p<0.01) in AA PCa tissues as compared to CA PCa tissues and might be related to the aggressiveness associated with AA population. In urine, miR-205 (p<0.05) and miR-214 (p<0.05) were significantly downregulated in PCa patients and can discriminate PCa patients from healthy individuals with 89% sensitivity and 80% specificity. In conclusion, present study showed that miR-205 and miR-214 are downregulated in PCa and may serve as potential non-invasive molecular biomarker for PCa.
authors.affiliation: Cancer Research Laboratory, Department of Biology, Chemistry and Physics, University of the District of Columbia, Washington, D. C., United States of America.
authors.forename: Anvesha
authors.initials: A
authors.lastname: Srivastava
authors.forename: Helle
authors.initials: H
authors.lastname: Goldberger
authors.forename: Alexander
authors.initials: A
authors.lastname: Dimtchev
authors.forename: Malathi
authors.initials: M
authors.lastname: Ramalinga
authors.forename: Juliet
authors.initials: J
authors.lastname: Chijioke
authors.forename: Catalin
authors.initials: C
authors.lastname: Marian
authors.forename: Eric K
authors.initials: EK
authors.lastname: Oermann
authors.forename: Sunghae
authors.initials: S
authors.lastname: Uhm
authors.forename: Joy S
authors.initials: JS
authors.lastname: Kim
authors.forename: Leonard N
authors.initials: LN
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Deborah L
authors.initials: DL
authors.lastname: Berry
authors.forename: Bhaskar V S
authors.initials: BV
authors.lastname: Kallakury
authors.forename: Subhash C
authors.initials: SC
authors.lastname: Chauhan
authors.forename: Sean P
authors.initials: SP
authors.lastname: Collins
authors.forename: Simeng
authors.initials: S
authors.lastname: Suy
authors.forename: Deepak
authors.initials: D
authors.lastname: Kumar
mesh_terms: D001741:Black or African American; D000368:Aged; D014408:Biomarkers, Tumor / Q000652:urine*; D015536:Down-Regulation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000652:urine*; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000652:urine*; D012334:RNA, Neoplasm / Q000652:urine*; D012680:Sensitivity and Specificity; D014481:United States; D044465:White People
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D012334:RNA, Neoplasm; C534425:MIR205, human; C547397:MIRN214 microRNA, human; C525709:MIR221, human