title: Expression of microRNA-218 in human pancreatic ductal adenocarcinoma and its correlation with tumor progression and patient survival.
abstract: BACKGROUND
  Our aim was to analyze clinicopathologic and prognostic values of microRNA (miR)-218 in pancreatic ductal adenocarcinima (PDAC).
  
  
  METHODS
  TaqMan quantitative RT-PCR was used to determine the expression of miR-218 in human PDAC cells and tissue samples. The association of miR-218 expression with clinicopathologic variables was analyzed. Kaplan-Meier survival analysis was performed to analyze the association of miR-218 expression with recurrence-free survival or overall survival of patients. Univariate and multivariate Cox regression analyses were performed.
  
  
  RESULTS
  The relative level of miR-218 in PDAC cells was significantly lower than that in normal human pancreatic duct epithelial cell line. Also, the mean level of miR-218 in PDAC tissues was significantly lower than that in normal pancreatic tissues. Statistical analyses indicated that low miR-218 expression was closely associated with poor tumor differentiation, advanced tumor stage, higher incidence of lymph node metastasis, and tumor recurrence. Kaplan-Meier survival analyses showed that patients with low miR-218 expression had lower recurrence-free and overall survival than those with high miR-218 expression. Univariate and multivariate Cox regression analyses showed that miR-218 might be an independent prognostic factor.
  
  
  CONCLUSIONS
  Reduced miR-218 in PDAC tissues was correlated with tumor progression, and might be an independent poor prognostic factor for patients.
authors.affiliation: Department of Hepatobiliary Surgery, First Hospital Affiliated to the Chinese PLA General Hospital, Beijing, People's Republic of China.
authors.forename: Ziman
authors.initials: Z
authors.lastname: Zhu
authors.forename: Yuefang
authors.initials: Y
authors.lastname: Xu
authors.forename: Jundong
authors.initials: J
authors.lastname: Du
authors.forename: Jingwang
authors.initials: J
authors.lastname: Tan
authors.forename: Huabao
authors.initials: H
authors.lastname: Jiao
mesh_terms: D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D018450:Disease Progression; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D015999:Multivariate Analysis; D009364:Neoplasm Recurrence, Local; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C547471:MIRN218 microRNA, human; D035683:MicroRNAs
keywords: microRNA-218; overall survival; pancreatic ductal adenocarcinoma; qRT-PCR; recurrence-free survival