title: Standard karyotype of Triticum umbellulatum and the characterization of derived chromosome addition and translocation lines in common wheat.
abstract: A standard karyotype and a generalized idiogram of Triticum umbellulatum (syn. Aegilops umbellulata, 2n = 2x = 14) was established based on C-banding analysis of ten accessions of different geographic origin and individual T. umbellulatum chromosomes in T. aestivum - T. umbellulatum chromosome addition lines. Monosomic (MA) and disomic (DA) T. aestivum - T. umbellulatum chromosome addition lines (DA1U = B, DA2U = D, MA4U = F, DA5U = C, DA6U = A, DA7U = E = G) and telosomic addition lines (DA1US, DA1UL, DA2US, DA2UL, DA4UL, MA5US, (+ iso 5US), DA5UL, DA7US, DA7UL) were analyzed. Line H was established as a disomic addition line for the translocated wheat - T. umbellulatum chromosome T2DS·4US. Radiation-induced wheat - T. umbellulatum translocation lines resistant to leaf rust (Lr9) were identified as T40 = T6BL·6BS-6UL, T41 = T4BL·4BS-6UL, T44 = T2DS·2DL-6UL, T47 = 'Transfer' = T6BS·6BL-6UL and T52 = T7BL·7BS-6UL. Breakpoints and sizes of the transferred T. umbellulatum segments in these translocations were determined by in situ hybridization analysis using total genomic T. umbellulatum DNA as a probe.
authors.affiliation: Department of Plant Pathology, Wheat Genetics Resource Center, Throckmorton Hall, Kansas State University, 66506-5502, Manhattan, KS, USA.
authors.forename: B
authors.initials: B
authors.lastname: Friebe
authors.forename: J
authors.initials: J
authors.lastname: Jiang
authors.forename: N
authors.initials: N
authors.lastname: Tuleen
authors.forename: B S
authors.initials: BS
authors.lastname: Gill