title: Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) co-transformed via particle bombardment.
abstract: Exploiting the biolistic process we have generated stable transgenic bean (Phaseolus vulgaris L.) plants with unlinked and linked foreign genes. Co-transformation was conducted using plasmid constructions containing a fusion of the gus and neo genes, which were co-introduced with the methionine-rich 2S albumin gene isolated from the Brazil nut and the antisense sequence of AC1, AC2, AC3 and BC1 genes from the bean golden mosaic geminivirus. The results revealed a co-transformation frequency ranging from 40% to 50% when using unlinked genes and 100% for linked genes. The introduced foreign genes were inherited in a Mendelian fashion in most of the transgenic bean lines. PCR and Southern blot hybridization confirmed the integration of the foreign genes in the plant genome.
authors.affiliation: Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia, EMBRAPA, P.O. Box 02372, 70849-970, Brasília, DF, Brazil.
authors.forename: F J
authors.initials: FJ
authors.lastname: Aragão
authors.forename: L M
authors.initials: LM
authors.lastname: Barros
authors.forename: A C
authors.initials: AC
authors.lastname: Brasileiro
authors.forename: S G
authors.initials: SG
authors.lastname: Ribeiro
authors.forename: F D
authors.initials: FD
authors.lastname: Smith
authors.forename: J C
authors.initials: JC
authors.lastname: Sanford
authors.forename: J C
authors.initials: JC
authors.lastname: Faria
authors.forename: E L
authors.initials: EL
authors.lastname: Rech