title: Novel GO-COO-β-CD/CA inclusion: its blood compatibility, antibacterial property and drug delivery.
abstract: GO-COO-β-CD/CA inclusion (carboxylated graphene-β-cyclodextrin/chlorhexidine acetate) was fabricated with a graphene-based drug carrier. The reaction time and ratio of carrier to drug were optimized by X-ray diffraction spectra to ensure the complete wrapping of CA. Hemolysis test and recalcification test demonstrated that the inclusion possessed good blood compatibility due to the inherent biocompatibility of β-CD molecules in the carrier. The inclusion displayed excellent inhibition effect on both gram negative bacteria of Escherichia coli and gram positive bacteria of Staphylococcus Aureus, while showing no cytotoxicity. More importantly, the drug efficiency was greatly improved with CA dosage as less as one-third of the pure drug due to the synergistic effect of the drug and carrier. Dynamic simulation implies that the delivery profile of CA from the inclusion is in accordance with the first-order dynamic equation, i.e. ln(1-Mt/M) = -kt.
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University , Nanjing , China .
authors.forename: Yinghong
authors.initials: Y
authors.lastname: Xiao
authors.forename: Yunting
authors.initials: Y
authors.lastname: Fan
authors.forename: Weiyang
authors.initials: W
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Gu
authors.forename: Ninglin
authors.initials: N
authors.lastname: Zhou
authors.forename: Jian
authors.initials: J
authors.lastname: Shen
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D001672:Biocompatible Materials / Q000008:administration & dosage*; D002460:Cell Line; D002710:Chlorhexidine / Q000008:administration & dosage*; D004337:Drug Carriers / Q000008:administration & dosage*; D016503:Drug Delivery Systems / Q000379:methods; D004926:Escherichia coli / Q000187:drug effects; D006108:Graphite / Q000008:administration & dosage*; D057809:HEK293 Cells; D006801:Humans; D012995:Solubility; D013211:Staphylococcus aureus / Q000187:drug effects; D014961:X-Ray Diffraction / Q000379:methods; D047392:beta-Cyclodextrins / Q000008:administration & dosage*
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D004337:Drug Carriers; D047392:beta-Cyclodextrins; D006108:Graphite; C031215:betadex; D002710:Chlorhexidine
keywords: Anti-bacteria; GO-COO-β-CD/CA inclusion; blood compatibility; drug carrier; drug delivery