title: Plant regeneration from protoplasts of Japanese lawngrass.
abstract: Embryogenic callus of Japanese lawngrass (Zoysia japonica Steud.) was induced from sterile mature seeds on LS medium with 5 mg / l of 2,4-D. Embryogenic callus selected visually under microscope was proliferated in liquid N6 medium with amino acids (N6-AA medium). Protoplasts were isolated from suspension cells by the treatment of enzyme mixture containing pectolyase Y-23 and cultured in K8p medium with 2 mg / l of 2,4-D at the density of 10(6) / ml. Plants were regenerated by transferring the protoplasts derived callus to MS medium and incubating at 28 °C under light for two months. Plantlets were successfully transplanted in the soil.
authors.affiliation: Japan Turfgrass Inc., 3-6-2 Akanehama, Narashino-shi, 275, Chiba, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Inokuma
authors.forename: K
authors.initials: K
authors.lastname: Sugiura
authors.forename: C
authors.initials: C
authors.lastname: Cho
authors.forename: R
authors.initials: R
authors.lastname: Okawara
authors.forename: S
authors.initials: S
authors.lastname: Kaneko