title: Monitoring the mineralisation of bone nodules in vitro by space- and time-resolved Raman micro-spectroscopy.
abstract: Raman microscopy was used as a label-free method to study the mineralisation of bone nodules formed by mesenchymal stem cells cultured in osteogenic medium in vitro. Monitoring individual bone nodules over 28 days revealed temporal and spatial changes in the crystalline phase of the hydroxyapatite components of the nodules.
authors.affiliation: School of Physics and Astronomy, University of Nottingham, Nottingham, NG7 2RD, UK. ioan.notingher@nottingham.ac.uk.
authors.forename: Adrian
authors.initials: A
authors.lastname: Ghita
authors.forename: Flavius C
authors.initials: FC
authors.lastname: Pascut
authors.forename: Virginie
authors.initials: V
authors.lastname: Sottile
authors.forename: Ioan
authors.initials: I
authors.lastname: Notingher
mesh_terms: D015519:Bone Density / Q000502:physiology*; D048429:Cell Size; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000502:physiology*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013997:Time Factors