title: Recovery and purification of rapamycin from the culture broth of Mtcc 5681.
abstract: In this study of the recovery and purification of rapamycin from the culture broth of an actinomycetes strain MTCC 5681, we investigated various factors such as biomass separation, suitable solvents for extraction, normal phase and flash chromatographic conditions and selective precipitation to obtain rapamycin in substantially pure form of the product. Adsorption chromatography particularly with normal phase and flash chromatography, in combination with centrifugal decantation is found to be the most suitable for separation as well as purification of rapamycin. Centrifugal decantation technique is likely to emerge as an efficient, industrially scalable, high yielding and economical process for biomass separation. The purity of rapamycin obtained through the method described was 99.4% which has not been reported so far.
authors.affiliation: Department of Biotechnology, Natco Research Center, Hyderabad-500018, India.
authors.forename: Polavarapu Baby
authors.initials: PB
authors.lastname: Rani
authors.forename: Battula Suneel
authors.initials: BS
authors.lastname: Kumar
authors.forename: A K S Bhujanga
authors.initials: AK
authors.lastname: Rao
authors.forename: S
authors.initials: S
authors.lastname: Sreenivasrao
authors.forename: Mangamoori Lakshmi
authors.initials: ML
authors.lastname: Narasu
mesh_terms: D039903:Actinobacteria / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D018533:Biomass; D002845:Chromatography / Q000379:methods*; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D005285:Fermentation; D020123:Sirolimus / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D003470:Culture Media; D020123:Sirolimus