title: Comparative gene expression profiling in human cumulus cells according to ovarian gonadotropin treatments.
abstract: In in vitro fertilization cycles, both HP-hMG and rFSH gonadotropin treatments are widely used to control human follicle development. The objectives of this study are (i) to characterize and compare gene expression profiles in cumulus cells (CCs) of periovulatory follicles obtained from patients stimulated with HP-hMG or rFSH in a GnRH antagonist cycle and (ii) to examine their relationship with in vitro embryo development, using Human Genome U133 Plus 2.0 microarrays. Genes that were upregulated in HP-hMG-treated CCs are involved in lipid metabolism (GM2A) and cell-to-cell interactions (GJA5). Conversely, genes upregulated in rFSH-treated CCs are implicated in cell assembly and organization (COL1A1 and COL3A1). Interestingly, some genes specific to each gonadotropin treatment (NPY1R and GM2A for HP-hMG; GREM1 and OSBPL6 for rFSH) were associated with day 3 embryo quality and blastocyst grade at day 5, while others (STC2 and PTX3) were related to in vitro embryo quality in both gonadotropin treatments. These genes may prove valuable as biomarkers of in vitro embryo quality.
authors.affiliation: Université Montpellier 1, UFR de Médecine, Montpellier, France ; CHU Montpellier, Institute for Research in Biotherapy, Hôpital Saint-Eloi, INSERM U1040, 34295 Montpellier, France.
authors.forename: Said
authors.initials: S
authors.lastname: Assou
authors.forename: Delphine
authors.initials: D
authors.lastname: Haouzi
authors.forename: Hervé
authors.initials: H
authors.lastname: Dechaud
authors.forename: Anna
authors.initials: A
authors.lastname: Gala
authors.forename: Alice
authors.initials: A
authors.lastname: Ferrières
authors.forename: Samir
authors.initials: S
authors.lastname: Hamamah
mesh_terms: D002986:Clinical Trials as Topic; D054885:Cumulus Cells / Q000378:metabolism*; D047108:Embryonic Development / Q000187:drug effects; D005260:Female; D005307:Fertilization in Vitro / Q000187:drug effects; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D006062:Gonadotropins / Q000008:administration & dosage*; D006801:Humans; D006080:Ovarian Follicle / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism
chemical_list: D006062:Gonadotropins; D005640:Follicle Stimulating Hormone