title: Harnessing the hydrolytic potential of phytopathogenic fungus Phoma exigua ITCC 2049 for saccharification of lignocellulosic biomass.
abstract: Phytopathogenic fungi develop unique systems for fast invasion by producing hydrolases, which may be explored as a source of hydrolytic enzymes for biofuel research. The present work deals with evaluation of a potato pathogen Phoma exigua ITCC 2049 for its potential to produce cellulase and xylanase enzyme. Taguchi methodology was applied to reveal the influence and contribution of five important factors (carbon source, organic and inorganic nitrogen source, surfactant, and pH) on hydrolytic enzyme production by Phoma. Cultivation of fungus under optimized condition produced endoglucanase (37.00 IU/ml), FPase (1.13 IU/ml), β-glucosidase (2.67 IU/ml) and xylanase (24.92 IU/ml) within 8 days of submerged fermentation. Saccharification of biopretreated Parthenium and paddy straw with cocktail of Phoma secretome supplemented with commercial β-glucosidase resulted in the significantly higher reducing sugar yield (651.04-698.11 mg/gds). This study proves the potential of Phoma as an alternative source of enzymes for biomass saccharification.
authors.affiliation: Division of Microbiology, Indian Agricultural Research Institute, New Delhi 110 012, India.
authors.forename: Rameshwar
authors.initials: R
authors.lastname: Tiwari
authors.forename: Surender
authors.initials: S
authors.lastname: Singh
authors.forename: Pawan K S
authors.initials: PK
authors.lastname: Nain
authors.forename: Sarika
authors.initials: S
authors.lastname: Rana
authors.forename: Anamika
authors.initials: A
authors.lastname: Sharma
authors.forename: Kumar
authors.initials: K
authors.lastname: Pranaw
authors.forename: Lata
authors.initials: L
authors.lastname: Nain
mesh_terms: D000704:Analysis of Variance; D001203:Ascomycota / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D018533:Biomass; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002244:Carbon / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D004798:Enzymes / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D008031:Lignin / Q000378:metabolism*; D009584:Nitrogen / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology; D013997:Time Factors
chemical_list: D003470:Culture Media; D004798:Enzymes; D005656:Fungal Proteins; D013501:Surface-Active Agents; C036909:lignocellulose; D002244:Carbon; D008031:Lignin; D009584:Nitrogen
keywords: Enzyme cocktail; Phoma exigua; Saccharification; Taguchi optimization