title: Fluorescence quenching and spectrophotometric methods for the determination of daunorubicin with meso-tera (4-sulphophenyl) porphyrin as probe.
abstract: In this work, a synthetic meso-tera (4-sulfophenyl) porphyrin (TPPS4) was used as a probe to determine daunorubicin (DNR) by fluorescence quenching and spectrophotometric methods. At pH 4.6 potassium acid phthalate-NaOH buffer solution, a 1:1 complex of DNR interacted with TPPS4 formed via the electrostatic attractions and hydrophobic interactions, thus resulted in TPPS4 fluorescence quenching and absorption spectra change. The maximum excitation wavelength (λex) and the maximum emission wavelength (λem) are 435 nm and 672 nm, respectively. The fluorescence quenching values (ΔF) are the good linear relationship to the concentration of DNR in the range of 0.8-6.0 mgL(-1). The method exhibits high sensitivity with the detection limit (3σ) being 27.0 ng mL(-1). Meanwhile, a decrease of absorbance is detected at 433 nm with the appearance of a new absorption peak at 420 nm. The optimum reaction conditions, influencing factors and the effect of coexisting substances have been investigated in our experiment. The results showed that the method had a good selectivity and could be applied to determine DNR in serum and urine samples. In addition, the combine ratio between DNR and TPPS4 was measured and the charge distribution before and after reaction was calculated by quantum chemistry calculation AM1 method. The type of fluorescence quenching was discussed by the absorption spectra change, Stern-Volmer plots and fluorescence lifetime determination.
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Shaopu
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Zhongfang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Chemical and Environmental Engineering, Chongqing Three Gorges University, Wanzhou, Chongqing 404100, China.
authors.forename: Jidong
authors.initials: J
authors.lastname: Yang
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Man
authors.initials: M
authors.lastname: Qiao
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: xiaolihu@swu.edu.cn.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Hu
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000032:analysis*; D003630:Daunorubicin / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D011166:Porphyrins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013053:Spectrophotometry / Q000379:methods
chemical_list: D000903:Antibiotics, Antineoplastic; D005456:Fluorescent Dyes; D011166:Porphyrins; C016136:tetraphenylporphine sulfonate; D003630:Daunorubicin
keywords: Absorption; Daunorubicin; Fluorescence quenching; Meso-tetrakis (4-sulfonatophenyl) porphyrin