title: Multiplex Real-Time Polymerase Chain Reaction-Based Method for the Rapid Detection of gyrA and parC Mutations in Quinolone-Resistant Escherichia coli and Shigella spp.
abstract: Two real-time polymerase chain reaction assays were developed to detect mutations in codons 83 and 87 in gyrA and in codons 80 and 91 in parC, the main sites that causes quinolone resistance in pathogenic Escherichia coli and Shigella spp. isolates. These assays can be employed as a useful method for controlling infections caused by quinolone-resistant E coli and Shigella isolates.
authors.affiliation: Division of Enteric Bacterial Infections, Korea National Institute of Health, Osong, Korea.
authors.forename: Junyoung
authors.initials: J
authors.lastname: Kim
authors.forename: Semi
authors.initials: S
authors.lastname: Jeon
authors.forename: Hyungjun
authors.initials: H
authors.lastname: Kim
authors.forename: Misun
authors.initials: M
authors.lastname: Park
authors.forename: Soobok
authors.initials: S
authors.lastname: Kim
authors.forename: Seonghan
authors.initials: S
authors.lastname: Kim
keywords: fluoroquinolone; gyrA; multiplex real-time PCR; parC