title: Protein kinase Darkener of apricot and its substrate EF1γ regulate organelle transport along microtubules.
abstract: Regulation of organelle transport along microtubules is important for proper distribution of membrane organelles and protein complexes in the cytoplasm. RNAi-mediated knockdown in cultured Drosophila S2 cells demonstrates that two microtubule-binding proteins, a unique isoform of Darkener of apricot (DOA) protein kinase, and its substrate, translational elongation factor EF1γ, negatively regulate transport of several classes of membrane organelles along microtubules. Inhibition of transport by EF1γ requires its phosphorylation by DOA on serine 294. Together, our results indicate a new role for two proteins that have not previously been implicated in regulation of the cytoskeleton. These results further suggest that the biological role of some of the proteins binding to the microtubule track is to regulate cargo transport along these tracks.
authors.affiliation: Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL 616011, USA.
authors.forename: Anna S
authors.initials: AS
authors.lastname: Serpinskaya
authors.forename: Karine
authors.initials: K
authors.lastname: Tuphile
authors.forename: Leonard
authors.initials: L
authors.lastname: Rabinow
authors.forename: Vladimir I
authors.initials: VI
authors.lastname: Gelfand
mesh_terms: D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D008264:Macrophages / Q000378:metabolism* / Q000648:ultrastructure; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D020648:Peptide Elongation Factor 1 / Q000235:genetics* / Q000378:metabolism; D020675:Peroxisomes / Q000378:metabolism*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D029721:Drosophila Proteins; D020648:Peptide Elongation Factor 1; D034741:RNA, Small Interfering; C089977:Doa protein, Drosophila; D017346:Protein Serine-Threonine Kinases
keywords: DOA Translation elongation factor EF1γ, EF1γ; Darkener of apricot; Drosophila melanogaster S2 cells; Organelle transport