title: Differential recovery of bacterial and archaeal 16S rRNA genes from ruminal digesta in response to glycerol as cryoprotectant.
abstract: Bacteria and archaea in frozen (-20°C) ruminal digesta were analysed by qPCR and cloning/sequencing of 16S rRNA genes. Samples frozen with and without glycerol as cryoprotectant indicated a major loss of Bacteroidetes in unprotected samples, resulting in higher proportions of Firmicutes. Archaeal numbers and diversity were unaffected.
authors.affiliation: Rowett Institute of Nutrition and Health, University of Aberdeen, Aberdeen, UK.
authors.forename: Nest
authors.initials: N
authors.lastname: McKain
authors.forename: Buğra
authors.initials: B
authors.lastname: Genc
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Snelling
authors.forename: R John
authors.initials: RJ
authors.lastname: Wallace
mesh_terms: D000818:Animals; D001105:Archaea / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005990:Glycerol / Q000494:pharmacology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics* / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction; D012417:Rumen / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013048:Specimen Handling / Q000379:methods
chemical_list: D003451:Cryoprotective Agents; D012336:RNA, Ribosomal, 16S; D005990:Glycerol
keywords: Cryoprotection; Glycerol; Rumen; Storage