title: Macrophage migration inhibitory factor and stearoyl-CoA desaturase 1: potential prognostic markers for soft tissue sarcomas based on bioinformatics analyses.
abstract: The diagnosis and treatment of soft tissue sarcomas (STSs) has been particularly difficult, because STSs are a group of highly heterogeneous tumors in terms of histopathology, histological grade, and primary site. Recent advances in genome technologies have provided an excellent opportunity to determine the complete biological characteristics of neoplastic tissues, resulting in improved diagnosis, treatment selection, and investigation of therapeutic targets. We had previously developed a novel bioinformatics method for marker gene selection and applied this method to gene expression data from STS patients. This previous analysis revealed that the extracted gene combination of macrophage migration inhibitory factor (MIF) and stearoyl-CoA desaturase 1 (SCD1) is an effective diagnostic marker to discriminate between subtypes of STSs with highly different outcomes. In the present study, we hypothesize that the combination of MIF and SCD1 is also a prognostic marker for the overall outcome of STSs. To prove this hypothesis, we first analyzed microarray data from 88 STS patients and their outcomes. Our results show that the survival rates for MIF- and SCD1-positive groups were lower than those for negative groups, and the p values of the log-rank test are 0.0146 and 0.00606, respectively. In addition, survival rates are more significantly different (p = 0.000116) between groups that are double-positive and double-negative for MIF and SCD1. Furthermore, in vitro cell growth inhibition experiments by MIF and SCD1 inhibitors support the hypothesis. These results suggest that the gene set is useful as a prognostic marker associated with tumor progression.
authors.affiliation: Graduate School of Horticulture, Chiba University, Matsudo, Chiba, Japan ; Graduate School of Bioscience and Biotechnology, Chubu University, Kasugai, Aichi, Japan ; Plant Biology Research Center, Chubu University, Kasugai, Aichi, Japan ; Division of Genetics, National Cancer Center Research Institute, Tokyo, Japan.
authors.forename: Hiro
authors.initials: H
authors.lastname: Takahashi
authors.forename: Robert
authors.initials: R
authors.lastname: Nakayama
authors.forename: Shuhei
authors.initials: S
authors.lastname: Hayashi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nemoto
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Murase
authors.forename: Koji
authors.initials: K
authors.lastname: Nomura
authors.forename: Teruyoshi
authors.initials: T
authors.lastname: Takahashi
authors.forename: Kenji
authors.initials: K
authors.lastname: Kubo
authors.forename: Shigetaka
authors.initials: S
authors.lastname: Marui
authors.forename: Koji
authors.initials: K
authors.lastname: Yasuhara
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Nakamura
authors.forename: Takuya
authors.initials: T
authors.lastname: Sueo
authors.forename: Anna
authors.initials: A
authors.lastname: Takahashi
authors.forename: Kaname
authors.initials: K
authors.lastname: Tsutsumiuchi
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Ohta
authors.forename: Akira
authors.initials: A
authors.lastname: Kawai
authors.forename: Shintaro
authors.initials: S
authors.lastname: Sugita
authors.forename: Shinjiro
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Honda
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Yoshida
authors.forename: Tadashi
authors.initials: T
authors.lastname: Hasegawa
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D019295:Computational Biology; D018572:Disease-Free Survival; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000096:biosynthesis*; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D020411:Oligonucleotide Array Sequence Analysis; D012509:Sarcoma / Q000378:metabolism / Q000401:mortality; D012983:Soft Tissue Neoplasms / Q000378:metabolism / Q000401:mortality; D013230:Stearoyl-CoA Desaturase / Q000096:biosynthesis*; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; D008263:Macrophage Migration-Inhibitory Factors; D009363:Neoplasm Proteins; C523776:SCD1 protein, human; D013230:Stearoyl-CoA Desaturase; D019746:Intramolecular Oxidoreductases; C515598:MIF protein, human