title: [Triptolide inhibits proliferation and induces apoptosis of imatinib resistant K562/G01 cells].
abstract: This study was aimed to explore the inhibitory effect of triptolide on proliferation and inducing apoptosis effect of K562/G01 cells and their possible mechanism. MTT assay was used to detect the effect of imatinib or triptolide alone and their combination on K562/G01 proliferation; the cell cycle, apoptosis rate, P-gp protein expression were detected by flow cytometry (FCM); the expression of P-gp was assessed by Western blot; the BCR/ABL gene expression was assayed by real time quantitative PCR. The results showed that triptolide could enhance the effect of imatinib on proliferation inhibition and apoptosis of K562/G01, arrested the cell cycle in G1 phase, down-regulated the expression of BCR/ABL gene and P-gp protein. It is concluded that triptolide induces K562/G01 cell proliferation inhibition and apoptosis, the mechanism may be related to cell cycle arrest, decrease of P-gp protein expression, inhibition of BCR/ABL gene expression.
authors.affiliation: Department of Hematology, Shangxi Big Hospital of Shangxi Medical University, Taiyuan 030032, Shangxi Province, China.
authors.forename: Si-Qun
authors.initials: SQ
authors.lastname: Wen
authors.forename: Liang-Ming
authors.initials: LM
authors.lastname: Ma
authors.forename: Yu-Jin
authors.initials: YJ
authors.lastname: Lu
authors.forename: Bo
authors.initials: B
authors.lastname: Bai
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D017209:Apoptosis / Q000187:drug effects*; D001549:Benzamides / Q000494:pharmacology; D059447:Cell Cycle Checkpoints; D049109:Cell Proliferation / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D004852:Epoxy Compounds / Q000494:pharmacology; D016044:Fusion Proteins, bcr-abl / Q000235:genetics; D006801:Humans; D000068877:Imatinib Mesylate; D020014:K562 Cells; D010616:Phenanthrenes / Q000494:pharmacology*; D010879:Piperazines / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; C581634:BCR-ABL1 fusion protein, human; D001549:Benzamides; D004224:Diterpenes; D004852:Epoxy Compounds; D010616:Phenanthrenes; D010879:Piperazines; D011743:Pyrimidines; C001899:triptolide; D000068877:Imatinib Mesylate; D016044:Fusion Proteins, bcr-abl