title: Subpicogram per milliliter detection of interleukins using silicon photonic microring resonators and an enzymatic signal enhancement strategy.
abstract: The detection of biomolecules at ultralow (low to subpicogram per milliliter) concentrations and within complex, clinically relevant matrices is a formidable challenge that is complicated by limitations imposed by the Langmuir binding isotherm and mass transport, for surface-based affinity biosensors. Here we report the integration of an enzymatic signal enhancement scheme onto a multiplexable silicon photonic microring resonator detection platform. To demonstrate the analytical value of this combination, we simultaneously quantitated levels of the interleukins IL-2, IL-6, and IL-8 in undiluted cerebrospinal fluid in an assay format that is multiplexable, relatively rapid (90 min), and features a 3 order of magnitude dynamic range and a limit of detection ≤1 pg/mL. The modular nature of this assay and technology should lend itself broadly amenable to different analyte classes, making it a versatile tool for biomarker analysis in clinically relevant settings.
authors.affiliation: Department of Chemistry, University of Illinois at Urbana-Champaign , 600 S. Matthews Ave., Urbana, IL 61801, United States.
authors.forename: Jared T
authors.initials: JT
authors.lastname: Kindt
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Luchansky
authors.forename: Abraham J
authors.initials: AJ
authors.lastname: Qavi
authors.forename: So-Hyun
authors.initials: SH
authors.lastname: Lee
authors.forename: Ryan C
authors.initials: RC
authors.lastname: Bailey
mesh_terms: D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D007376:Interleukin-2 / Q000134:cerebrospinal fluid*; D015850:Interleukin-6 / Q000134:cerebrospinal fluid*; D016209:Interleukin-8 / Q000134:cerebrospinal fluid*; D017785:Photons; D012825:Silicon / Q000737:chemistry*
chemical_list: D007376:Interleukin-2; D015850:Interleukin-6; D016209:Interleukin-8; D012825:Silicon