title: Simultaneous and direct determination of iron and nickel in biological solid samples by high-resolution continuum source graphite furnace atomic absorption spectrometry.
abstract: The simultaneous and direct determination of nickel and iron in plants and lichens has been investigated using high-resolution continuum source graphite furnace atomic absorption spectrometry. The primary resonance line for nickel at 232.003 nm and the adjacent secondary line for iron at 232.036 nm have been used for this purpose. The optimization of the experimental conditions was performed using a pine needles certified reference material (SRM 1575a). The influence of pyrolysis and atomization temperatures, the amount of solid sample introduced into the graphite furnace and the use of aqueous or solid standard for calibration were studied. The spectral interferences caused by absorption of the concomitants of the solid sample were detected and corrected using a least square algorithm. Aliquots of 0.1-1mg of the solid samples were weighed onto the solid sampling platforms and analyzed directly, without addition of any reagents. The limits of detection were 25 µg kg(-1) for nickel and 0.40 mg kg(-1) for iron and the precision, expressed as the relative standard deviation, ranged from 7% to 12%. The proposed method was used to determine both metals in different bioindicator samples with successful results.
authors.affiliation: Departamento de Química Analítica y Análisis Instrumental, Facultad de Ciencias, Avda. Francisco Tomás y Valiente 7, Universidad Autónoma de Madrid, 28049 Madrid, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Gómez-Nieto
authors.forename: Ma Jesús
authors.initials: MJ
authors.lastname: Gismera
authors.forename: Ma Teresa
authors.initials: MT
authors.lastname: Sevilla
authors.forename: Jesús R
authors.initials: JR
authors.lastname: Procopio
mesh_terms: D002138:Calibration; D006358:Hot Temperature; D007501:Iron / Q000032:analysis*; D016018:Least-Squares Analysis; D008011:Lichens / Q000737:chemistry*; D057230:Limit of Detection; D009532:Nickel / Q000032:analysis*; D041605:Pinus sylvestris / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D012015:Reference Standards; D013054:Spectrophotometry, Atomic / Q000379:methods*
chemical_list: D009532:Nickel; D007501:Iron
keywords: Bioindicator; Direct solid sample analysis; High-resolution continuum source GF AAS; Simultaneous Fe and Ni determination