title: O-glycosylation of NnTreg lymphocytes recognized by the Amaranthus leucocarpus lectin.
abstract: O-glycosidically-linked glycans have been involved in development, maturation, homing, and immune regulation in T cells. Previous reports indicate that Amaranthus leucocarpus lectin (ALL), specific for glycans containing galactose-N-acetylgalactosamine and N-acetylgalactosamine, recognizes human naïve CD27(+)CD25(+)CD4(+) T cells. Our aim was to evaluate the phenotype of CD4(+) T cells recognized by ALL in peripheral blood mononuclear cells obtained from healthy volunteers. CD4(+) T cells were isolated by negative selection using magnetic beads-labeled monoclonal antibodies; the expression of T regulatory cell phenotypic markers was assessed on ALL-recognized cells. In addition, IL-4, IL-10, IFN-γ, and TGF-β intracellular production in ALL (+) cells was also evaluated. The analyses of phenotypic markers and intracellular cytokines were performed through flow cytometry. ALL-recognized CD4(+) T cells were mainly CD45RA(+), CCR7(+) cells. Although 52 ± 10% CD25(+)Foxp3(+) cells were positive to ALL, only 34 ± 4% of ALL (+) cells corresponded to CD25(+)Foxp3(-) cells. Intracellular cytokines in freshly obtained ALL (+)CD4(+) T cells exhibited 8% of IL-4, 15% of IL-10, 2% of IFN-γ, and 15% of TGF-β, whereas ALL (-)CD4(+) T cells depicted 1% of IL-4, 2% of IL-10, <1% of IFN-γ, and 6% of TGF-β. Our results show that galactose-N-acetylgalactosamine and N-galactosamine-bearing CD4(+) T cells expressed phenotypic markers of NnTreg cells.
authors.affiliation: Unidad de Investigación, Instituto de Oftalmología Fundación Conde de Valenciana, 06800 México, DF, Mexico ; Laboratorio de Inmunología, Departamento de Bioquímica, Facultad de Medicina, (UNAM), P.O. Box 70159, 04510 México, DF, Mexico.
authors.forename: María C
authors.initials: MC
authors.lastname: Jiménez-Martínez
authors.forename: Ricardo
authors.initials: R
authors.lastname: Lascurain
authors.forename: Aniela
authors.initials: A
authors.lastname: Méndez-Reguera
authors.forename: Sergio
authors.initials: S
authors.lastname: Estrada-Parra
authors.forename: Iris
authors.initials: I
authors.lastname: Estrada-García
authors.forename: Patricia
authors.initials: P
authors.lastname: Gorocica
authors.forename: Salvador
authors.initials: S
authors.lastname: Martínez-Cairo
authors.forename: Edgar
authors.initials: E
authors.lastname: Zenteno
authors.forename: Raúl
authors.initials: R
authors.lastname: Chávez
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D060908:CTLA-4 Antigen / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006023:Glycoproteins / Q000276:immunology* / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D016130:Immunophenotyping; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D018655:Lymphocyte Count; D010641:Phenotype; D037121:Plant Lectins / Q000276:immunology* / Q000378:metabolism*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: C121359:Amaranthus leucocarpus lectin; D060908:CTLA-4 Antigen; D016207:Cytokines; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D006023:Glycoproteins; D053645:Interleukin-2 Receptor alpha Subunit; D037121:Plant Lectins; D016212:Transforming Growth Factor beta