title: RAPD discloses high molecular diversity of Mycobacterium tuberculosis from Michoacan, Mexico.
abstract: Random amplified polymorphism DNA (RAPD) is an easy, inexpensive technique for the characterization of pathogens in low-income countries. In this study we used RAPD to assess the genetic diversity of a small collection of isolates of mycobacteria from the Mexican state of Michoacan. In contrast with the low annual tuberculosis incidence in Michoacan relative to the national average, we found a high molecular diversity value suggesting high population diversity of M. tuberculosis in the studied region. Our findings justify further typing efforts with other molecular tools such as MIRU-VNTR and spoligotyping.
authors.affiliation: Centro Multidisciplinario de Estudios en Biotecnologia, Facultad de Medicina;Veterinaria y Zootecnia, Universidad Michoacana de San Nicolas de Hidalgo.
authors.forename: A L
authors.initials: AL
authors.lastname: Guill N-Nepita
authors.forename: G
authors.initials: G
authors.lastname: Vazquez-Marrufo
authors.forename: F T
authors.initials: FT
authors.lastname: Blanco-Guillot
authors.forename: G A
authors.initials: GA
authors.lastname: Figueroa-Aguilar
authors.forename: M S
authors.initials: MS
authors.lastname: Vazquez-Garciduenas
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015373:Bacterial Typing Techniques / Q000379:methods*; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D014376:Tuberculosis / Q000453:epidemiology / Q000382:microbiology*; D055815:Young Adult