title: Oxidative stress, inflammatory biomarkers, and toxicity in mouse lung and liver after inhalation exposure to 100% biodiesel or petroleum diesel emissions.
abstract: Over the past decade, soy biodiesel (BD) has become a first alternative energy source that is economically viable and meets requirements of the Clean Air Act. Due to lower mass emissions and reduced hazardous compounds compared to diesel combustion emissions (CE), BD exposure is proposed to produce fewer adverse health effects. However, considering the broad use of BD and its blends in different industries, this assertion needs to be supported and validated by mechanistic and toxicological data. Here, adverse effects were compared in lungs and liver of BALB/cJ mice after inhalation exposure (0, 50, 150, or 500 μg/m3; 4 h/d, 5 d/wk, for 4 wk) to CE from 100% biodiesel (B100) and diesel (D100). Compared to D100, B100 CE produced a significant accumulation of oxidatively modified proteins (carbonyls), an increase in 4-hydroxynonenal (4-HNE), a reduction of protein thiols, a depletion of antioxidant gluthatione (GSH), a dose-related rise in the levels of biomarkers of tissue damage (lactate dehydrogenase, LDH) in lungs, and inflammation (myeloperoxidase, MPO) in both lungs and liver. Significant differences in the levels of inflammatory cytokines interleukin (IL)-6, IL-10, IL-12p70, monocyte chemoattractant protein (MCP)-1, interferon (IFN) γ, and tumor necrosis factor (TNF)-α were detected in lungs and liver upon B100 and D100 CE exposures. Overall, the tissue damage, oxidative stress, inflammation, and cytokine response were more pronounced in mice exposed to BD CE. Further studies are required to understand what combustion products in BD CE accelerate oxidative and inflammatory responses.
authors.affiliation: a Health Effects Laboratory Division, Pathology and Physiology Research Branch , National Institute of Occupational and Safety Health , Morgantown , West Virginia , USA.
authors.forename: Anna A
authors.initials: AA
authors.lastname: Shvedova
authors.forename: Naveena
authors.initials: N
authors.lastname: Yanamala
authors.forename: Ashley R
authors.initials: AR
authors.lastname: Murray
authors.forename: Elena R
authors.initials: ER
authors.lastname: Kisin
authors.forename: Timur
authors.initials: T
authors.lastname: Khaliullin
authors.forename: Meghan K
authors.initials: MK
authors.lastname: Hatfield
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Tkach
authors.forename: Q T
authors.initials: QT
authors.lastname: Krantz
authors.forename: David
authors.initials: D
authors.lastname: Nash
authors.forename: Charly
authors.initials: C
authors.lastname: King
authors.forename: M
authors.initials: M
authors.lastname: Ian Gilmour
authors.forename: Stephen H
authors.initials: SH
authors.lastname: Gavett
mesh_terms: D000280:Administration, Inhalation; D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D056804:Biofuels; D015415:Biomarkers / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D019570:Inhalation Exposure / Q000009:adverse effects*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000378:metabolism; D001335:Vehicle Emissions / Q000633:toxicity*
chemical_list: D000393:Air Pollutants; D056804:Biofuels; D015415:Biomarkers; D016207:Cytokines; D001335:Vehicle Emissions; D007770:L-Lactate Dehydrogenase; D009195:Peroxidase