title: Transformation of the mitochondrial genome.
abstract: Although mitochondrial transformation is highly desirable in mammals and plants, it is only possible in two unicellular organisms, the budding yeast Saccharomyces cerevisiae and the unicellular green alga Chlamydomonas reinhardtii. Here, we give an overview of the attempts made to transform mitochondria of mammals and plants and the possible reasons for their failure. This review briefly describes the mitochondrial transformation principles in yeast and describes in more detail the transformation and its applications in Chlamydomonas.
authors.affiliation: Genetics of Microorganisms, Department of Life Sciences, Institute of Botany, University of Liege, Liege, Belgium.
authors.forename: Véronique
authors.initials: V
authors.lastname: Larosa
authors.forename: Claire
authors.initials: C
authors.lastname: Remacle
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism; D018744:DNA, Plant / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods; D005821:Genetic Techniques; D054629:Genome, Mitochondrial; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010944:Plants / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D019076:Transgenes
chemical_list: D004272:DNA, Mitochondrial; D018744:DNA, Plant