title: The utility of the BIOMED-2 primers in the detection of 2 clonal, B-lymphoproliferative disorders simultaneously involving the same site.
abstract: BACKGROUND
  Molecular tests for clonality performed on atypical lymphoid lesions may yield abnormal results because of the coexistence of monoclonal B lymphocytosis or monoclonal gammopathy of undetermined significance in the sample investigated.
  
  
  OBJECTIVE
  To investigate the ability of the BIOMED-2 sets of primers to identify 2 clonal populations in the same formalin-fixed, paraffin-embedded tissue sample. Design.-Ten cases with 2 B-lymphoproliferative disorders at the same site were analyzed using 5 BIOMED-2 primer sets (IGH FR1, FR2, FR3, IGK VJ, and IGK VKde).
  
  
  RESULTS
  All 10 cases (100%) showed at least 1 clone; 8 of 10 cases (80%) had 2 clones with at least 1 primer set, and the 2 clones were shown by 4 or 5 primer sets in none of the cases (0%), by 3 sets in 1 of 10 cases (10%), by 2 sets in 4 of 10 cases (40%), and by 1 set in 3 of 10 cases (30%). The most effective set was IGH FR2, detecting 4 of 10 biclonal cases (40%). The IGK VJ and IGK VKde each showed 2 clones in 3 of 10 cases (30% each). The least effective sets were IGH FR1 and FR3, with 2 of 10 cases (20%) each, with IGH FR1 being the least useful.
  
  
  CONCLUSIONS
  The BIOMED-2 primers are effective in the detection of 2 clonal populations in the same sample.
authors.affiliation: From the RJ Tomsich Pathology and Laboratory Medicine Institute, Cleveland Clinic, Cleveland, Ohio.
authors.forename: Brenda
authors.initials: B
authors.lastname: Ly
authors.forename: Claudiu V
authors.initials: CV
authors.lastname: Cotta
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology*; D017931:DNA Primers / Q000235:genetics*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D016130:Immunophenotyping; D008218:Lymphocytosis / Q000175:diagnosis / Q000235:genetics / Q000276:immunology; D008232:Lymphoproliferative Disorders / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology*; D010265:Paraproteinemias / Q000175:diagnosis / Q000235:genetics / Q000276:immunology; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D007143:Immunoglobulin Heavy Chains; D007145:Immunoglobulin kappa-Chains