title: Effects of speckle/pixel size ratio on temporal and spatial speckle-contrast analysis of dynamic scattering systems: Implications for measurements of blood-flow dynamics.
abstract: Laser Speckle Contrast Imaging (LSCI) is an optical technique used to generate blood flow maps with high spatial and temporal resolution. It is well known that in LSCI, the speckle size must exceed the Nyquist criterion to maximize the speckle's pattern contrast. In this work, we study experimentally the effect of speckle-pixel size ratio not only in dynamic speckle contrast, but also on the calculation of the relative flow speed for temporal and spatial analysis. Our data suggest that the temporal LSCI algorithm is more accurate at assessing the relative changes in flow speed than the spatial algorithm.
authors.affiliation: Optics Department, INAOE, Puebla, Mexico ; Beckman Laser Institute and Medical Clinic, Department of Surgery, University of California, Irvine, 1002 Health Sciences Road East, Irvine, California 92612, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Ramirez-San-Juan
authors.forename: E
authors.initials: E
authors.lastname: Mendez-Aguilar
authors.forename: N
authors.initials: N
authors.lastname: Salazar-Hermenegildo
authors.forename: A
authors.initials: A
authors.lastname: Fuentes-Garcia
authors.forename: R
authors.initials: R
authors.lastname: Ramos-Garcia
authors.forename: B
authors.initials: B
authors.lastname: Choi
keywords: (030.6140) Speckle; (120.6150) Speckle imaging; (120.7250) Velocimetry