title: Effect of human adipose tissue-derived mesenchymal-stem-cell bioactive materials on porcine embryo development.
abstract: Human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) secrete bioactive materials that are beneficial for tissue repair and regeneration. In this study, we characterized human hAT-MSC bioactive material (hAT-MSC-BM), and examined the effect of hAT-MSC-BM on porcine embryo development. hAT-MSC-BM was enriched with several growth factors and cytokines, including fibroblast growth factor 2 (FGF2), vascular endothelial growth factor A (VEGFA), and interleukin 6 (IL6). Among the various concentrations and days of treatment tested, 10% hAT-MSC-BM treatment beginning on culture Day 4 provided the best environment for the in vitro growth of parthenogenetic porcine embryos. While the addition of 10% fetal bovine serum (FBS) increased the hatching rate and the total cell number of parthenogenetic porcine embryos compared with the control and hAT-MSC culture medium group, the best results were from the group cultured with 10% hAT-MSC-BM. Mitochondrial activity was also higher in the 10% hAT-MSC-BM-treated group. Moreover, the relative mRNA expression levels of development and anti-apoptosis genes were significantly higher in the 10% hAT-MSC-BM-treated group than in control, hAT-MSC culture medium, or 10% FBS groups, whereas the transcript abundance of an apoptosis gene was slightly lower. Treatment with 10% hAT-MSC-BM starting on Day 4 also improved the development rate and the total cell number of in vitro-fertilized embryos. This is the first report on the benefits of hAT-MSC-BM in a porcine embryo in vitro culture system. We conclude that hAT-MSC-BM is a new, alternative supplement that can improve the development of porcine embryos during both parthenogenesis and fertilization in vitro.
authors.affiliation: Miraebio Research Institute, Mirae Biotech, Seoul, Korea; Jeju National University Stem Cell Research Center, Jeju National University, Jeju, Korea.
authors.forename: Hyo-Young
authors.initials: HY
authors.lastname: Park
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Kim
authors.forename: Seung-Eun
authors.initials: SE
authors.lastname: Lee
authors.forename: Hyun-Yong
authors.initials: HY
authors.lastname: Choi
authors.forename: Jeremiah Jiman
authors.initials: JJ
authors.lastname: Moon
authors.forename: Min-Jee
authors.initials: MJ
authors.lastname: Park
authors.forename: Yeo-Jin
authors.initials: YJ
authors.lastname: Son
authors.forename: Jun-Beom
authors.initials: JB
authors.lastname: Lee
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Jeong
authors.forename: Dong-Sun
authors.initials: DS
authors.lastname: Lee
authors.forename: Key-Jung
authors.initials: KJ
authors.lastname: Riu
authors.forename: Se-Pill
authors.initials: SP
authors.lastname: Park
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000187:drug effects*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D010312:Parthenogenesis; D011506:Proteins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D013552:Swine / Q000196:embryology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism / Q000494:pharmacology
chemical_list: D003470:Culture Media; C508600:IL6 protein, human; D015850:Interleukin-6; D011506:Proteins; D012333:RNA, Messenger; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2