title: A novel zinc finger protein 219-like (ZNF219L) is involved in the regulation of collagen type 2 alpha 1a (col2a1a) gene expression in zebrafish notochord.
abstract: The notochord is required for body plan patterning in vertebrates, and defects in notochord development during embryogenesis can lead to diseases affecting the adult. It is therefore important to elucidate the gene regulatory mechanism underlying notochord formation. In this study, we cloned the zebrafish zinc finger 219-like (ZNF219L) based on mammalian ZNF219, which contains nine C2H2-type zinc finger domains. Through whole-mount in situ hybridization, we found that znf219L mRNA is mainly expressed in the zebrafish midbrain-hindbrain boundary, hindbrain, and notochord during development. The znf219L morpholino knockdown caused partial abnormal notochord phenotype and reduced expression of endogenous col2a1a in the notochord specifically. In addition, ZNF219L could recognize binding sites with GGGGG motifs and trigger augmented activity of the col2a1a promoter in a luciferase assay. Furthermore, in vitro binding experiments revealed that ZNF219L recognizes the GGGGG motifs in the promoter region of the zebrafish col2a1a gene through its sixth and ninth zinc finger domains. Taken together, our results reveal that ZNF219L is involved in regulating the expression of col2a1a in zebrafish notochord specifically.
authors.affiliation: 1. Institute of Fisheries Sciences, National Taiwan University, Taipei 106, Taiwan;
authors.forename: Huang-Wei
authors.initials: HW
authors.lastname: Lien
authors.forename: Chung-Hsiang
authors.initials: CH
authors.lastname: Yang
authors.forename: Chia-Hsiung
authors.initials: CH
authors.lastname: Cheng
authors.forename: Chin-Chun
authors.initials: CC
authors.lastname: Hung
authors.forename: Wei-Hao
authors.initials: WH
authors.lastname: Liao
authors.forename: Pung-Pung
authors.initials: PP
authors.lastname: Hwang
authors.forename: Yu-San
authors.initials: YS
authors.lastname: Han
authors.forename: Chang-Jen
authors.initials: CJ
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019521:Body Patterning / Q000235:genetics; D003001:Cloning, Molecular; D024043:Collagen Type II / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D009672:Notochord / Q000378:metabolism*; D016415:Sequence Alignment; D017384:Sequence Deletion; D014157:Transcription Factors / Q000502:physiology*; D015027:Zebrafish / Q000235:genetics*; D029961:Zebrafish Proteins / Q000502:physiology*
chemical_list: D024043:Collagen Type II; D014157:Transcription Factors; D029961:Zebrafish Proteins; C588040:znf219 protein, zebrafish
keywords: collagen type 2 alpha 1a; notochord; transcriptional regulation.; zebrafish; zinc finger protein 219