title: Microscale diffusion measurements and simulation of a scaffold with a permeable strut.
abstract: Electrospun nanofibrous structures provide good performance to scaffolds in tissue engineering. We measured the local diffusion coefficients of 3-kDa FITC-dextran in line patterns of electrospun nanofibrous structures fabricated by the direct-write electrospinning (DWES) technique using the fluorescence recovery after photobleaching (FRAP) method. No significant differences were detected between DWES line patterns fabricated with polymer supplied at flow rates of 0.1 and 0.5 mL/h. The oxygen diffusion coefficients of samples were estimated to be ~92%-94% of the oxygen diffusion coefficient in water based on the measured diffusion coefficient of 3-kDa FITC-dextran. We also simulated cell growth and distribution within spatially patterned scaffolds with struts consisting of either oxygen-permeable or non-permeable material. The permeable strut scaffolds exhibited enhanced cell growth. Saturated depths at which cells could grow to confluence were 15% deeper for the permeable strut scaffolds than for the non-permeable strut scaffold.
authors.affiliation: Department of Mechanical System Engineering, Graduate School of Knowledge-Based Technology and Energy, Korea Polytechnic University, Jeongwang-dong, Siheung-si, Gyeonggi-do 429-793, Korea. songwan@kpu.ac.kr.
authors.forename: Seung Youl
authors.initials: SY
authors.lastname: Lee
authors.forename: Byung Ryong
authors.initials: BR
authors.lastname: Lee
authors.forename: Jongwan
authors.initials: J
authors.lastname: Lee
authors.forename: Seongjun
authors.initials: S
authors.lastname: Kim
authors.forename: Jung Kyung
authors.initials: JK
authors.lastname: Kim
authors.forename: Young Hun
authors.initials: YH
authors.lastname: Jeong
authors.forename: Songwan
authors.initials: S
authors.lastname: Jin
mesh_terms: D002452:Cell Count; D049109:Cell Proliferation; D003911:Dextrans / Q000737:chemistry; D004058:Diffusion; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry*; D010100:Oxygen / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D003911:Dextrans; C015219:fluorescein isothiocyanate dextran; D016650:Fluorescein-5-isothiocyanate; D010100:Oxygen