title: Characterization of a complete genome of a circular single-stranded DNA virus from porcine stools in Korea.
abstract: Porcine circular single-stranded DNA viruses have been just identified from swine feces in Korea. This virus was mentioned as bovine stool-associated circular DNA virus (BoSCV)-like virus discovered from porcine stools. However, the thorough characteristics of the virus were not identified. Therefore, this research focuses on finding a full genome sequence and analyzing the genetic features of the virus. The virus, now called porcine stool-associated circular DNA virus in Korea (PoSCV Kor), consists of 2,589 bases forming circular structure. It has two major ORFs inversely encoding replicase and capsid protein, with each stem-loop structure between 5' ends and 3' ends of the two putative ORFs. This characteristics is the same as PoSCV in New Zealand, but different from chimpanzee stool-associated circular virus (ChiSCVs) and BoSCV, which have one stem-loop structure. Therefore, it would be sure that PoSCV Kor is very similar to PoSCV in respect to the genetic aspect; the same number of nucleotide bases and the amino acid identity of replicase and capsid protein (96 and 93 %, respectively). This fact could be certified through the finding that PoSCV Kor and PoSCV are in the same cluster by phylogenetic analysis based on the comparison with full-sequences of other circular ssDNA viruses.
authors.affiliation: Department of Veterinary Medicine Virology Laboratory, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, Korea.
authors.forename: A Reum
authors.initials: AR
authors.lastname: Kim
authors.forename: Hee Chun
authors.initials: HC
authors.lastname: Chung
authors.forename: Hye Kwon
authors.initials: HK
authors.lastname: Kim
authors.forename: Eun Ok
authors.initials: EO
authors.lastname: Kim
authors.forename: Van Giap
authors.initials: VG
authors.lastname: Nguyen
authors.forename: Min Gyung
authors.initials: MG
authors.lastname: Choi
authors.forename: Hye Jung
authors.initials: HJ
authors.lastname: Yang
authors.forename: Jung Ah
authors.initials: JA
authors.lastname: Kim
authors.forename: Bong Kyun
authors.initials: BK
authors.lastname: Park
mesh_terms: D036022:Capsid Proteins / Q000235:genetics; D016000:Cluster Analysis; D004266:DNA Virus Infections / Q000662:veterinary*; D004267:DNA Viruses / Q000235:genetics* / Q000302:isolation & purification; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D005243:Feces / Q000821:virology*; D016679:Genome, Viral; D007723:Korea; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D036022:Capsid Proteins; D004277:DNA, Single-Stranded; D004279:DNA, Viral; C023140:DNA replicase; D004259:DNA-Directed DNA Polymerase