title: Porcine endogenous retrovirus infection changes the expression of inflammation-related genes in lipopolysaccharide-stimulated human dermal fibroblasts.
abstract: BACKGROUND
  The present study focuses on explaining the interaction between porcine endogenous retroviruses (PERVs) and human cells in inflammatory conditions. The differences in expression of selected inflammation-related genes in human dermal fibroblasts (NHDF) infected with PERVs with and without lipopolysaccharide stimulation were identified.
  
  
  METHODS
  The PERV infectivity was analyzed using a co-culture of NHDF and PK15 cells. Quantification of PERV A, B DNA and PERV A, B RNA was performed by real-time QPCR and QRT-PCR. The analysis of the expression profile was performed using HG-U133A 2.0 oligonucleotide microarrays.
  
  
  RESULTS
  PERV infection of NHDF cells with LPS stimulation resulted in a statistically significant decrease in the copy number of PERV A DNA, and an increase in the copy number of PERV A RNA compared to fibroblasts without stimulation. There was no statistically significant difference between the copy number of PERV B RNA of LPStreated and untreated NHDF cells. Typing of differentiation genes was performed in a panel of 571 selected transcripts of inflammation-related genes. Among all studied genes, 23 were differentially regulated with a change greater that 1.1-fold and p<0.05 in all studied groups. Of these 23 genes, 3 were found to be regulated by more than 2.0-fold at least in 2 studied groups (IL6, IL8, and IL33).
  
  
  CONCLUSIONS
  The interaction between porcine endogenous retroviruses and human cells changes in inflammatory conditions. PERV infection of NHDF cells may alter the expression of inflammation-related genes. Further investigations concerning PERV infection of human cells in different conditions seem to be necessary.
authors.affiliation: Department of Molecular Biology, Medical University of Silesia, Sosnowiec, Poland.
authors.forename: Magdalena C
authors.initials: MC
authors.lastname: Kimsa
authors.forename: Barbara
authors.initials: B
authors.lastname: Strzałka-Mrozik
authors.forename: Małgorzata W
authors.initials: MW
authors.lastname: Kimsa
authors.forename: Celina
authors.initials: C
authors.lastname: Kruszniewska-Rajs
authors.forename: Joanna
authors.initials: J
authors.lastname: Gola
authors.forename: Jolanta
authors.initials: J
authors.lastname: Adamska
authors.forename: Antoni
authors.initials: A
authors.lastname: Rajs
authors.forename: Urszula
authors.initials: U
authors.lastname: Mazurek
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000235:genetics; D020077:Endogenous Retroviruses; D005347:Fibroblasts / Q000378:metabolism* / Q000821:virology; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D012192:Retroviridae Infections / Q000378:metabolism*; D013552:Swine
chemical_list: D004279:DNA, Viral