title: An ultrasensitive quantum dots fluorescent polarization immunoassay based on the antibody modified Au nanoparticles amplifying for the detection of adenosine triphosphate.
abstract: In this work, an ultrasensitive fluorescent polarization immunoassay (FPIA) method based on the quantum dot/aptamer/antibody/gold nanoparticles ensemble has been developed for the detection of adenosine triphosphate (ATP). DNA hybridization is formed when ATP is present in the PBS solution containing the DNA-conjugated quantum dots (QDs) and antibody-AuNPs. The substantial sensitivity improvement of the antibody-AuNPs-enhanced method is mainly attributed to the slower rotation of fluorescent unit when QDs-labeled oligonucleotides hybridize with antibody modified the gold nanoparticle. As a result, the fluorescent polarization (FP) values of the system increase significantly. Under the optimal conditions, a linear response with ATP concentration is ranged from 8×10(-12) M to 2.40×10(-4) M. The detection limit reached as low as 1.8 pM. The developed work provides a sensitive and selective immunoassay protocol for ATP detection, which could be applied in more bioanalytical systems.
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering of Guangxi Normal University, Guilin 541004, China.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: He
authors.forename: Jianniao
authors.initials: J
authors.lastname: Tian
authors.forename: Kun
authors.initials: K
authors.lastname: Hu
authors.forename: Juanni
authors.initials: J
authors.lastname: Zhang
authors.forename: Sheng
authors.initials: S
authors.lastname: Chen
authors.forename: Yixuan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Shulin
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D019187:Cadmium Compounds / Q000737:chemistry; D016231:Fluorescence Polarization Immunoassay / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000911:Antibodies, Monoclonal; D019187:Cadmium Compounds; D018036:Selenium Compounds; D013440:Sulfides; C034939:cadmium sulfide; D006046:Gold; D000255:Adenosine Triphosphate; C058667:cadmium selenide
keywords: Adenosine triphosphate; Au nanoparticles; Fluorescent polarization immunoassay; Quantum dots