title: Coupling of HPLC with electrospray ionization mass spectrometry for studying the aging of ultrasmall multifunctional gadolinium-based silica nanoparticles.
abstract: Sub-5 nm multimodal nanoparticles have great potential for theranostic applications due to their easy renal elimination combined with complementary imaging properties and therapeutic facilities. Their potential clinical use requires the full characterization of not only the nanoparticle but also all its possible degradation products. We have recently proposed new ultrasmall gadolinium-based nanoparticles for multimodal imaging and radiosensitization. The aim of this article is to describe an analytical tool to characterize degradation products in a highly diluted medium. We demonstrate that HPLC coupled to electrospray ionization mass spectrometry (ESI-MS) can be used in order to determine precisely the composition of nanoparticles and their degradation fragments during aging.
authors.affiliation: Institut Lumière Matière, UMR5306 Université Lyon 1-CNRS, Université de Lyon 69622 Villeurbanne cedex, France.
authors.forename: Charles
authors.initials: C
authors.lastname: Truillet
authors.forename: François
authors.initials: F
authors.lastname: Lux
authors.forename: Olivier
authors.initials: O
authors.lastname: Tillement
authors.forename: Philippe
authors.initials: P
authors.lastname: Dugourd
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Antoine
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005682:Gadolinium / Q000737:chemistry*; D053758:Nanoparticles; D012822:Silicon Dioxide; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D012822:Silicon Dioxide; D005682:Gadolinium