title: Real-time bioluminescent tracking of cellular population dynamics.
abstract: Cellular population dynamics are routinely monitored across many diverse fields for a variety of purposes. In general, these dynamics are assayed either through the direct counting of cellular aliquots followed by extrapolation to the total population size, or through the monitoring of signal intensity from any number of externally stimulated reporter proteins. While both viable methods, here we describe a novel technique that allows for the automated, non-destructive tracking of cellular population dynamics in real-time. This method, which relies on the detection of a continuous bioluminescent signal produced through expression of the bacterial luciferase gene cassette, provides a low cost, low time-intensive means for generating additional data compared to alternative methods.
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.
authors.forename: Dan
authors.initials: D
authors.lastname: Close
authors.forename: Tingting
authors.initials: T
authors.lastname: Xu
authors.forename: Steven
authors.initials: S
authors.lastname: Ripp
authors.forename: Gary
authors.initials: G
authors.lastname: Sayler
mesh_terms: D000818:Animals; D002460:Cell Line; D002477:Cells / Q000166:cytology*; D003584:Cytological Techniques / Q000379:methods*; D008156:Luciferases / Q000235:genetics; D008163:Luminescent Measurements / Q000379:methods*; D013997:Time Factors; D014162:Transfection
chemical_list: D008156:Luciferases