title: [The protective effect of DR2 activation on hypoxia/reperfusion injury in the neonatal rat cardiomyocytes and related mechanism].
abstract: OBJECTIVE
  To observe the effect of dopamine receptor (DR2) activation on hypoxia/reperfusion injury (HRI) in the neonatal rat cardiomyocytes, and to explore its mechanism.
  
  
  METHODS
  The hypoxia/reperfusion (H/R) injury model was established in primarily cultured neonatal rat cardiomyocytes, and randomly assigned: control, H/R, bromocriptine (Bro) and haloperidol (Hal) groups. The cell apoptosis was detected using inverted microscope, transmission electron microscope and flow cytometry (FCM). The lactate dehydrogenase(LDH) and superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in cell medium were analyzed. The expression of mRNA and protein of caspase-3, caspase-8, caspase-9, Fas, Fas-L, Cyt C and Bcl-2 were detected by RT-PCR and Western blot, respectively.
  
  
  RESULTS
  Compared with the control group, apoptosis rate, LDH activity, MDA content and the expression of pro-apoptotic factors and anti-apoptotic factors were increased, but SOD activity was decreased in H/R group. Compared with the H/R group, all index above-mentioned were down-regulated or reversed in Bro-group, and had no obvious differences in Hal-group.
  
  
  CONCLUSIONS
  The neonatal rat cardiomyocytes injury and apoptosis caused by hypoxia/reperfusion can be inhibited with DR2 activation, which mechanism is related to scavenging oxygen radical.
authors.affiliation: Department of Pathophysiology, Harbin Medical University, Harbin 150086, China.
authors.forename: Can
authors.initials: C
authors.lastname: Wei
authors.forename: Jun
authors.initials: J
authors.lastname: Gao
authors.forename: Ai-Dong
authors.initials: AD
authors.lastname: Chen
authors.forename: Shu-Zhi
authors.initials: SZ
authors.lastname: Bai
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Hong-Jiang
authors.initials: HJ
authors.lastname: Shao
authors.forename: Xue
authors.initials: X
authors.lastname: Peng
authors.forename: Mei-Xiu
authors.initials: MX
authors.lastname: Li
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Xu
authors.forename: Hong-Zhu
authors.initials: HZ
authors.lastname: Li
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D015687:Cell Hypoxia; D015428:Myocardial Reperfusion Injury / Q000209:etiology / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D017448:Receptors, Dopamine D2 / Q000378:metabolism*
chemical_list: D017448:Receptors, Dopamine D2