title: Comparison of rpoB gene sequencing, 16S rRNA gene sequencing, gyrB multiplex PCR, and the VITEK2 system for identification of Acinetobacter clinical isolates.
abstract: Since accurate identification of species is necessary for proper treatment of Acinetobacter infections, we compared the performances of 4 bacterial identification methods using 167 Acinetobacter clinical isolates to identify the best identification method. To secure more non-baumannii Acinetobacter (NBA) strains as target strains, we first identified Acinetobacter baumannii in a total of 495 Acinetobacter clinical isolates identified using the VITEK 2 system. Because 371 of 495 strains were identified as A. baumannii using gyrB multiplex 1 PCR and blaOXA51-like PCR, we performed rpoB gene sequencing and 16S rRNA gene sequencing on remaining 124 strains belonging to NBA and 52 strains of A. baumannii. For identification of Acinetobacter at the species level, the accuracy rates of rpoB gene sequencing, 16S rRNA gene sequencing, gyrB multiplex PCR, and the VITEK 2 were 98.2%, 93.4%, 77.2%, and 35.9%, respectively. The gyrB multiplex PCR seems to be very useful for the detection of ACB complex because its concordance rates to the final identification of strains of ACB complex were 100%. Both the rpoB gene sequencing and the 16S rRNA gene sequencing may be useful in identifying Acinetobacter.
authors.affiliation: Research Center for Resistant Cells, Chosun University Medical School, Gwang-Ju, South Korea.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Lee
authors.affiliation: Research Center for Resistant Cells, Chosun University Medical School, Gwang-Ju, South Korea; Department of Laboratory Medicine, Chosun University Medical School, Gwang-Ju, South Korea. Electronic address: sjbjang@chosun.ac.kr.
authors.forename: Sook Jin
authors.initials: SJ
authors.lastname: Jang
authors.affiliation: Research Center for Resistant Cells, Chosun University Medical School, Gwang-Ju, South Korea; Department of Internal Medicine, Yantaishan Hospital, Yantai, China.
authors.forename: Xue Min
authors.initials: XM
authors.lastname: Li
authors.affiliation: Department of Laboratory Medicine, Chosun University Medical School, Gwang-Ju, South Korea.
authors.forename: Geon
authors.initials: G
authors.lastname: Park
authors.affiliation: Korean Collection for Oral Microbiology and Department of Oral Biochemistry, College of Dentistry, Chosun University, Gwang-Ju, South Korea.
authors.forename: Joong-Ki
authors.initials: JK
authors.lastname: Kook
authors.affiliation: Korean Collection for Oral Microbiology and Department of Oral Biochemistry, College of Dentistry, Chosun University, Gwang-Ju, South Korea.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Korean Collection for Type Cultures, Biological Resources Center, Korea Research Institute of Bioscience and Biotechnology, Daejon, South Korea.
authors.forename: Young-Hyo
authors.initials: YH
authors.lastname: Chang
authors.affiliation: Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, South Korea.
authors.forename: Jong Hee
authors.initials: JH
authors.lastname: Shin
authors.affiliation: Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, South Korea.
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, Chosun University Medical School, Gwang-Ju, South Korea.
authors.forename: Dong-Min
authors.initials: DM
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, Chosun University Medical School, Gwang-Ju, South Korea.
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Kang
authors.affiliation: Department of Laboratory Medicine, Chosun University Medical School, Gwang-Ju, South Korea.
authors.forename: Dae-Soo
authors.initials: DS
authors.lastname: Moon
mesh_terms: D000150:Acinetobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000151:Acinetobacter Infections / Q000175:diagnosis* / Q000382:microbiology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015373:Bacterial Typing Techniques / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D027081:DNA Gyrase / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D027081:DNA Gyrase
keywords: 16S; Acinetobacter; DNA; DNA Gyrase; Genes; Identification; PCR; RNA; Ribosomal; Sequence analysis; rpoB