title: Molecular cloning and characterization of a newly isolated pyrethroid-degrading esterase gene from a genomic library of Ochrobactrum anthropi YZ-1.
abstract: A novel pyrethroid-degrading esterase gene pytY was isolated from the genomic library of Ochrobactrum anthropi YZ-1. It possesses an open reading frame (ORF) of 897 bp. Blast search showed that its deduced amino acid sequence shares moderate identities (30% to 46%) with most homologous esterases. Phylogenetic analysis revealed that PytY is a member of the esterase VI family. pytY showed very low sequence similarity compared with reported pyrethroid-degrading genes. PytY was expressed, purified, and characterized. Enzyme assay revealed that PytY is a broad-spectrum degrading enzyme that can degrade various pyrethroids. It is a new pyrethroid-degrading gene and enriches genetic resource. Kinetic constants of Km and Vmax were 2.34 mmol·L(-1) and 56.33 nmol min(-1), respectively, with lambda-cyhalothrin as substrate. PytY displayed good degrading ability and stability over a broad range of temperature and pH. The optimal temperature and pH were of 35°C and 7.5. No cofactors were required for enzyme activity. The results highlighted the potential use of PytY in the elimination of pyrethroid residuals from contaminated environments.
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China ; Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Science, Beijing, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Ruan
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhai
authors.forename: Jinlong
authors.initials: J
authors.lastname: Song
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Shi
authors.forename: Kang
authors.initials: K
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Yan
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004950:Esterases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial / Q000235:genetics; D015698:Genomic Library; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007477:Ions; D007700:Kinetics; D008670:Metals / Q000494:pharmacology; D008969:Molecular Sequence Data; D020621:Ochrobactrum anthropi / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D010802:Phylogeny; D011722:Pyrethrins / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity / Q000187:drug effects; D013696:Temperature; D014170:Transformation, Genetic / Q000187:drug effects
chemical_list: D007477:Ions; D008670:Metals; D011722:Pyrethrins; D004950:Esterases