title: Detection of microRNA maturation using unmodified pre-microRNA and branched rolling circle amplification.
abstract: The ever-increasing number of different miRNAs and their association with a vast number of cellular dysfunctions and diseases have initiated several groups to investigate miRNA maturation, which ultimately leads to down regulation of a target messenger RNA (mRNA) and its downstream product. A rapid, convenient, and reliable assay to detect the Dicer-mediated miRNA-maturation step may facilitate research in this field. Here we describe the in vitro detection of the Dicer-mediated miRNA maturation step using unmodified pre-miRNA and branched rolling circle amplification.
authors.affiliation: Institute for Chemistry, Humboldt Universität zu Berlin, Berlin, Germany.
authors.forename: Saskia
authors.initials: S
authors.lastname: Neubacher
authors.forename: Christoph
authors.initials: C
authors.lastname: Arenz
mesh_terms: D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010766:Phosphorylation; D059367:RNA Cleavage; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism*; D043244:Ribonuclease III / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D004277:DNA, Single-Stranded; D035683:MicroRNAs; D012322:RNA Precursors; D043244:Ribonuclease III