title: Effects of mulberry ethanol extracts on hydrogen peroxide-induced oxidative stress in pancreatic β-cells.
abstract: Reactive oxygen species (ROS) are key mediators of mammalian cellular damage and are associated with diseases such as aging, arteriosclerosis, inflammation, rheumatoid arthritis and diabetes. Type 1 diabetes develops upon the destruction of pancreatic β-cells, which is partly due to ROS activity. In this study, we investigated the cytoprotective and anti-oxidative effects of fractionated mulberry extracts in mouse insulin-producing pancreatic β-cells (MIN6N cells). Treatment with hydrogen peroxide (H2O2) induced significant cell death and increased intracellular ROS levels, lipid peroxidation and DNA fragmentation in the MIN6N cells. Fractionated mulberry extracts significantly reduced the H2O2-dependent production of intracellular ROS, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and lipid peroxidation. In addition, mulberry extracts inhibited DNA fragmentation induced by H2O2. Thus, the antioxidant properties of mulberry extracts in pancreatic β-cells may be exploited for the prevention or treatment of type 1 diabetes.
authors.affiliation: Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Kangwon‑do 200‑701, Republic of Korea.
authors.forename: Young Rae
authors.initials: YR
authors.lastname: Kim
authors.forename: Jong Seok
authors.initials: JS
authors.lastname: Lee
authors.forename: Ki Rim
authors.initials: KR
authors.lastname: Lee
authors.forename: Young Eon
authors.initials: YE
authors.lastname: Kim
authors.forename: Nam In
authors.initials: NI
authors.lastname: Baek
authors.forename: Eock Kee
authors.initials: EK
authors.lastname: Hong
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D053938:DNA Fragmentation / Q000187:drug effects; D000431:Ethanol; D006861:Hydrogen Peroxide / Q000009:adverse effects*; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D031625:Morus / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D000431:Ethanol; D006861:Hydrogen Peroxide