title: Mapping a new nematode resistance locus in Lycopersicon peruvianum.
abstract: Accessions of the wild tomato species L. peruvianum were screened with a root-knot nematode population (557R) which infects tomato plants carrying the nematode resistance gene Mi. Several accessions were found to carry resistance to 557R. A L. peruvianum backcross population segregating for resistance to 557R was produced. The segregation ratio of resistant to susceptible plants suggested that a single, dominant gene was a major factor in the new resistance. This gene, which we have designated Mi-3, confers resistance against nematode strains that can infect plants carrying Mi. Mi-3, or a closely linked gene, also confers resistance to nematodes at 32°C, a temperature at which Mi is not effective. Bulked-segregant analysis with resistant and susceptible DNA pools was employed to identify RAPD markers linked to this gene. Five-hundred-and-twenty oligonucleotide primers were screened and two markers linked to the new resistance gene were identified. One of the linked markers (NR14) was mapped to chromosome 12 of tomato in an L. esculentum/L. pennellii mapping population. Linkage of NR14 and Mi-3 with RFLP markers known to map on the short arm of chromosome 12 was confirmed by Southern analysis in the population segregating for Mi-3. We have positioned Mi-3 near RFLP marker TG180 which maps to the telomeric region of the short arm of chromosome 12 in tomato.
authors.affiliation: Center for Engineering Plants for Resistance Against Pathogens, University of California, 95616, Davis, CA, USA.
authors.forename: J
authors.initials: J
authors.lastname: Yaghoobi
authors.forename: I
authors.initials: I
authors.lastname: Kaloshian
authors.forename: Y
authors.initials: Y
authors.lastname: Wen
authors.forename: V M
authors.initials: VM
authors.lastname: Williamson