title: How ticks get under your skin: insertion mechanics of the feeding apparatus of Ixodes ricinus ticks.
abstract: The tick Ixodes ricinus uses its mouthparts to penetrate the skin of its host and to remain attached for about a week, during which time Lyme disease spirochaetes may pass from the tick to the host. To understand how the tick achieves both tasks, penetration and attachment, with the same set of implements, we recorded the insertion events by cinematography, interpreted the mouthparts' function by scanning electron microscopy and identified their points of articulation by confocal microscopy. Our structural dynamic observations suggest that the process of insertion and attachment occurs via a ratchet-like mechanism with two distinct stages. Initially, the two telescoping chelicerae pierce the skin and, by moving alternately, generate a toehold. Subsequently, a breaststroke-like motion, effected by simultaneous flexure and retraction of both chelicerae, pulls in the barbed hypostome. This combination of a flexible, dynamic mechanical ratchet and a static holdfast thus allows the tick to solve the problem of how to penetrate skin and also remain stuck for long periods of time.
authors.affiliation: Abteilung Parasitologie, Institut für Pathologie, Charité Universitätsmedizin Berlin, , Malteserstrasse 74-100, 12249 Berlin, Germany, Laboratory of Public Health Entomology, Department of Immunology and Infectious Diseases, Harvard School of Public Health, , 665 Huntington Avenue, Boston, MA 02115, USA, Department of Organismic and Evolutionary Biology, School of Engineering and Applied Sciences, Harvard University, , 29 Oxford Street, Cambridge, MA 02138, USA.
authors.forename: Dania
authors.initials: D
authors.lastname: Richter
authors.forename: Franz-Rainer
authors.initials: FR
authors.lastname: Matuschka
authors.forename: Andrew
authors.initials: A
authors.lastname: Spielman
authors.forename: L
authors.initials: L
authors.lastname: Mahadevan
mesh_terms: D000818:Animals; D005247:Feeding Behavior; D006790:Host-Parasite Interactions; D018884:Ixodes / Q000033:anatomy & histology / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D008812:Mice, Hairless
keywords: Ixodes tick; attachment; mouthparts; ratchet