title: Removal potential of anti-estrogenic activity in secondary effluents by coagulation.
abstract: Anti-estrogenic activity in wastewater is gaining increased attention because of its endocrine-disrupting function. In this study, the level and removal efficiency by coagulation of anti-estrogenic activity in secondary effluents of domestic wastewater treatment plants were studied. Anti-estrogenic activity was detected in secondary effluent samples at a tamoxifen (TAM) equivalent concentration level of 0.38-0.94 mg-TAML(-1). Dissolved organic matters (DOM) with the molecular weight (MW) less than 3000 Da in hydrophobic acids (HOA) and hydrophobic neutrals (HON) fractions of the secondary effluent were the key fractions related to anti-estrogenic activity. Coagulation with FeCl(3) and polyaluminium chloride (PAC) can remove the anti-estrogenic activity of the secondary effluents, but the removal efficiency was limited. The removal efficiency using FeCl(3) coagulant was higher than that induced by PAC. Dissolved organic carbon was continuously removed with increased coagulant dose (0-120 mg L(-1) FeCl(3) or 0-60 mg L(-1) PAC). However, the removal of anti-estrogenic activity was not enhanced further when the coagulant concentration was beyond a critical value (30 mg L(-1) FeCl(3) or 10 mg L(-1) PAC). The highest removal of anti-estrogenic activity was about 36% by FeCl(3) and 20% by PAC. Size exclusion chromatography results indicated difficulty in removing DOM with MW less than 3000 Da in the secondary effluent during coagulation even at a high coagulant concentration, which led to low removal efficiency of anti-estrogenic activity.
authors.affiliation: Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (MARC), School of Environment, Tsinghua University, Beijing 100084, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Tang
authors.forename: Qian-Yuan
authors.initials: QY
authors.lastname: Wu
authors.forename: Huang
authors.initials: H
authors.lastname: Huang
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Hu
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
mesh_terms: D004965:Estrogen Antagonists / Q000032:analysis*; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*; D018508:Water Purification / Q000379:methods*
chemical_list: D004965:Estrogen Antagonists; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Anti-estrogenic activity; Coagulation; Dissolved organic matter; Fractionation; Secondary effluent; Size exclusion chromatography