title: Cationically rendered biopolymer surfaces for high protein affinity support matrices.
abstract: The use of cationic biopolymer surfaces for high protein binding affinity matrices is described. As model proteins, fluorescently labeled bovine serum albumins (FITC-BSA, TRITC-BSA) have been employed. The amount of proteins on such cationically rendered surfaces was quantified by QCM-D. In addition, flexible, transparent, patterned COP slides have been prepared and loaded with proteins ranging from 15 pM to 15 μM TRITC-BSA.
authors.affiliation: University of Maribor, Institute for the Engineering and Design of Materials, Smetanova Ulica 17, 2000 Maribor, Slovenia. karin.stana@um.si.
authors.forename: Tamilselvan
authors.initials: T
authors.lastname: Mohan
authors.forename: Tijana
authors.initials: T
authors.lastname: Ristić
authors.forename: Rupert
authors.initials: R
authors.lastname: Kargl
authors.forename: Ales
authors.initials: A
authors.lastname: Doliska
authors.forename: Stefan
authors.initials: S
authors.lastname: Köstler
authors.forename: Volker
authors.initials: V
authors.lastname: Ribitsch
authors.forename: Jure
authors.initials: J
authors.lastname: Marn
authors.forename: Stefan
authors.initials: S
authors.lastname: Spirk
authors.forename: Karin
authors.initials: K
authors.lastname: Stana-Kleinschek
mesh_terms: D000327:Adsorption; D001704:Biopolymers / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives* / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D001704:Biopolymers; C118071:N-trimethyl chitosan chloride; D012235:Rhodamines; C039217:fluorescein isothiocyanate bovine serum albumin; D012710:Serum Albumin, Bovine; C009434:tetramethylrhodamine isothiocyanate; D002482:Cellulose; D048271:Chitosan; D016650:Fluorescein-5-isothiocyanate