title: Whey peptides prevent chronic ultraviolet B radiation-induced skin aging in melanin-possessing male hairless mice.
abstract: Whey proteins or peptides exhibit various actions, including an antioxidant action, an anticancer action, and a protective action against childhood asthma and atopic syndrome. The effects of orally administered whey peptides (WPs) on chronic ultraviolet B (UVB) radiation-induced cutaneous changes, including changes in cutaneous thickness, elasticity, wrinkle formation, etc., have not been examined. In this study, we studied the preventive effects of WPs on cutaneous aging induced by chronic UVB irradiation in melanin-possessing male hairless mice (HRM). UVB (36-180 mJ/cm(2)) was irradiated to the dorsal area for 17 wk in HRM, and the measurements of cutaneous thickness and elasticity in UVB irradiated mice were performed every week. WPs (200 and 400 mg/kg, twice daily) were administered orally for 17 wk. WPs inhibited the increase in cutaneous thickness, wrinkle formation, and melanin granules and the reduction in cutaneous elasticity associated with photoaging. Furthermore, it has been reported that UVB irradiation-induced skin aging is closely associated with the increase in expression of matrix metalloproteinase (MMP), vascular endothelial growth factor (VEGF), Ki-67-, and 8-hydroxy-2'-deoxyguanosine (8-OHdG)-positive cells. WPs also prevented increases in the expression of MMP-2 and pro-MMP-9, VEGF, and Ki-67- and 8-OHdG-positive cells induced by chronic UVB irradiation. It was found that WPs prevent type IV collagen degradation, angiogenesis, proliferation, and DNA damage caused by UVB irradiation. Overall, these results demonstrate the considerable benefit of WPs for protection against solar UV-irradiated skin aging as a supplemental nutrient.
authors.affiliation: Division of Biochemical Pharmacology, Department of Basic Medical Research, and.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kimura
authors.forename: Maho
authors.initials: M
authors.lastname: Sumiyoshi
authors.forename: Toshiya
authors.initials: T
authors.lastname: Kobayashi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D001808:Blood Vessels / Q000187:drug effects / Q000378:metabolism; D024141:Collagen Type IV / Q000378:metabolism; D004249:DNA Damage / Q000528:radiation effects; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D019587:Dietary Supplements; D004792:Enzyme Precursors / Q000235:genetics / Q000378:metabolism; D019394:Ki-67 Antigen / Q000235:genetics / Q000378:metabolism; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008812:Mice, Hairless; D008894:Milk Proteins / Q000008:administration & dosage*; D015595:Skin Aging / Q000187:drug effects* / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism; D000067816:Whey Proteins
chemical_list: D000975:Antioxidants; D024141:Collagen Type IV; D004792:Enzyme Precursors; D019394:Ki-67 Antigen; D008894:Milk Proteins; C498355:Mki67 protein, mouse; D042461:Vascular Endothelial Growth Factor A; D000067816:Whey Proteins; C467480:vascular endothelial growth factor A, mouse; D000080242:8-Hydroxy-2'-Deoxyguanosine; C120487:pro-matrix metalloproteinase 9; D020778:Matrix Metalloproteinase 2; C522362:Mmp2 protein, mouse; D020780:Matrix Metalloproteinase 9; D003849:Deoxyguanosine