title: A high-performance glucose biosensor using covalently immobilised glucose oxidase on a poly(2,6-diaminopyridine)/carbon nanotube electrode.
abstract: A highly-sensitive glucose biosensor amenable to ultra-miniaturisation was fabricated by immobilisation of glucose oxidase (GOx), onto a poly(2,6-diaminopyridine)/multi-walled carbon nanotube/glassy carbon electrode (poly(2,6-DP)/MWNT/GCE). Cyclic voltammetry was used for both the electrochemical synthesis of poly-(2,6-DP) on the surface of a MWNT-modified GC electrode, and characterisation of the polymers deposited on the GC electrode. The synergistic effect of the high active surface area of both the conducting polymer, i.e., poly-(2,6-DP) and MWNT gave rise to a remarkable improvement in the electrocatalytic properties of the biosensor. The transfer coefficient (α), heterogeneous electron transfer rate constant and Michaelis-Menten constant were calculated to be 0.6, 4 s(-1) and 0.20 mM at pH 7.4, respectively. The GOx/poly(2,6-DP)/MWNT/GC bioelectrode exhibited two linear responses to glucose in the concentration ranging from 0.42 μM to 8.0 mM with a correlation coefficient of 0.95, sensitivity of 52.0 μA mM(-1) cm(-2), repeatability of 1.6% and long-term stability, which could make it a promising bioelectrode for precise detection of glucose in the biological samples.
authors.affiliation: Biosensors and Bioelectronics Centre, Department of Physics, Chemistry and Biology, IFM-Linköping University, S-58183 Linköping, Sweden; Department of Chemistry, Faculty of Sciences, University of Zanjan, P.O. Box 45195-313, Zanjan, Islamic Republic of Iran. Electronic address: makamyabi@gmail.com.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ali Kamyabi
authors.forename: Nasim
authors.initials: N
authors.lastname: Hajari
authors.forename: Anthony P F
authors.initials: AP
authors.lastname: Turner
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Tiwari
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D005949:Glucose Oxidase / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D037742:Nanotubes, Carbon / Q000737:chemistry*; D058105:Polymerization; D011725:Pyridines / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001786:Blood Glucose; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D011725:Pyridines; D005949:Glucose Oxidase; C505797:2,6-diaminopyridine
keywords: Direct electron transfer; Glucose biosensor; Glucose oxidase; MWNT/poly-(2,6-diaminopyridine)