title: Availability of a fetal goat tongue cell line ZZ-R 127 for isolation of Foot-and-mouth disease virus (FMDV) from clinical samples collected from animals experimentally infected with FMDV.
abstract: The availability of the fetal goat tongue cell line ZZ-R 127 for the isolation of Foot-and-mouth disease virus (FMDV) has not been evaluated using clinical samples other than epithelial suspensions. Therefore, in the current study, the availability of ZZ-R 127 cells for the isolation of FMDV was evaluated using clinical samples (e.g., sera, nasal swabs, saliva, feces, and oropharyngeal fluids) collected from animals experimentally infected with an FMDV isolate. Virus isolation rates for the ZZ-R 127 cells were statistically higher than those for the porcine kidney cell line (IB-RS-2) in experimental infections using cattle, goats, and pigs (P < 0.01). Virus titers in the ZZ-R 127 cells were also statistically higher than those in the IB-RS-2 cells. The availability of ZZ-R 127 cells for the isolation of FMDV not only from epithelial suspensions but also from other clinical samples was confirmed in the current study.
authors.affiliation: 1Toru Kanno, Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1 Josui-honcho, Kodaira, Tokyo 187-0022, Japan. kannot@affrc.go.jp.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Fukai
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Onozato
authors.forename: Rie
authors.initials: R
authors.lastname: Kitano
authors.forename: Reiko
authors.initials: R
authors.lastname: Yamazoe
authors.forename: Kazuki
authors.initials: K
authors.lastname: Morioka
authors.forename: Manabu
authors.initials: M
authors.lastname: Yamada
authors.forename: Seiichi
authors.initials: S
authors.lastname: Ohashi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yoshida
authors.forename: Toru
authors.initials: T
authors.lastname: Kanno
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000821:virology*; D002460:Cell Line; D016009:Chi-Square Distribution; D003588:Cytopathogenic Effect, Viral / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000821:virology; D005536:Foot-and-Mouth Disease / Q000175:diagnosis / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000302:isolation & purification*; D015511:Goat Diseases / Q000175:diagnosis / Q000821:virology*; D006041:Goats; D009296:Nasal Cavity / Q000821:virology; D009960:Oropharynx / Q000821:virology; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012463:Saliva / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000821:virology*
chemical_list: D012367:RNA, Viral
keywords: Cell line; Foot-and-mouth disease virus; isolation