title: Hyperhomocysteinemia and of Methylenetetrahydrofolate Reductase (C677T) Genetic Polymorphism in Patients with Deep Vein Thrombosis.
abstract: OBJECTIVE
  To determine the concentration of total plasma homocysteine (tHcy) as well as different genotypes of methylenetetrahydrofolate reductase MTHFR (C677T) in healthy subjects and patients with deep vein thrombosis (DVT).
  
  
  METHODS
  The investigation comprised a total of 160 subjects divided in two main groups: 80 healthy subjects (control group) and 80 patients with deep vein thrombosis. Concentration of tHcy was determined by spectrophotometric cyclic enzymatic method and mutation of MTHFR (C677T) gene was examined by polymerase chain reaction according to Schneider.
  
  
  RESULTS
  The results obtained for plasma tHcy in the control group were 11.62±3.43 μmol/L, while tHcy level was significantly higher in patients with deep vein thrombosis as compared to the control group, 15.19±3.63 μmol/L (р<0.001). The analysis of the results has shown that MTHFR (C677T) genetic polymorphism was responsible for mild to moderate hyperhomocysteinemia in the majority of subjects.
  
  
  CONCLUSIONS
  The level of tHcy in the examined patients was significantly higher in comparison with the control group. Multiple regression analysis has shown that tHcy level in CT and TT genotypes of MTHFR (C677T) was statistically higher in comparison with CC genotype of MTHFR (C677T) in both, the control group and the DVT patients.
authors.affiliation: Institute of Medical and Experimental Biochemistry, Medical Faculty, Skopje, Republic of Macedonia.
authors.forename: Julijana
authors.initials: J
authors.lastname: Brezovska-Kavrakova
authors.forename: Marija
authors.initials: M
authors.lastname: Krstevska
authors.forename: Gordana
authors.initials: G
authors.lastname: Bosilkova
authors.forename: Sonja
authors.initials: S
authors.lastname: Alabakovska
authors.forename: Saso
authors.initials: S
authors.lastname: Panov
authors.forename: Nikola
authors.initials: N
authors.lastname: Orovchanec
keywords: MTHFR (C677T) gene; deep vein thrombosis.; total plasma homocysteine