title: Analysis of yeast DNA by alkaline filter elution.
abstract: The method of analysis of DNA in mammalian cells by alkaline elution from filters (Kohn et al. 1974) was adapted for studies on yeast DNA. By this technique spheroplasts obtained from yeast cells are lysed on filters and single-stranded DNA fragments selectively eluted by alkaline solutions. The procedure was applied to monitor the occurrence of replication intermediates and production of DNA single-strand breakage by MMS, and its repair in growth medium.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Rakowiecka 36, 02-532, Warsaw, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Zuk
authors.forename: D
authors.initials: D
authors.lastname: Zaborowska
authors.forename: Z
authors.initials: Z
authors.lastname: Swietlińska