title: Becker muscular dystrophy-like myopathy regarded as so-called "fatty muscular dystrophy" in a pig: a case report and its diagnostic method.
abstract: We describe a case of human Becker muscular dystrophy (BMD)-like myopathy that was characterized by the declined stainability of dystrophin at sarcolemma in a pig and the immunostaining for dystrophin on the formalin-fixed, paraffin-embedded (FFPE) tissue. The present case was found in a meat inspection center. The pig looked appeared healthy at the ante-mortem inspection. Muscular abnormalities were detected after carcass dressing as pale, discolored skeletal muscles with prominent fat infiltrations and considered so-called "fatty muscular dystrophy". Microscopic examination revealed following characteristics: diffused fat infiltration into the skeletal muscle and degeneration and regeneration of the remaining skeletal muscle fibers. Any lesions that were suspected of neurogenic atrophy, traumatic muscular degeneration, glycogen storage disease or other porcine muscular disorders were not observed. The immunostaining for dystrophin was conducted and confirmed to be applicable on FFPE porcine muscular tissues and revealed diminished stainability of dystrophin at the sarcolemma in the present case. Based on the histological observations and immunostaining results, the present case was diagnosed with BMD-like myopathy associated with dystrophin abnormality in a pig. Although the genetic properties were not clear, the present BMD-like myopathy implied the occurrence of dystrophinopathy in pigs. To the best of our knowledge, this is the first report of a natural case of myopathy associated with dystrophin abnormalities in a pig.
authors.affiliation: Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Horiuchi
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Aihara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mizutani
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kousaka
authors.forename: Tsuneyuki
authors.initials: T
authors.lastname: Nagafuchi
authors.forename: Mariko
authors.initials: M
authors.lastname: Ochiai
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ochiai
authors.forename: Yoshiyasu
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Furuoka
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Asai
authors.forename: Koji
authors.initials: K
authors.lastname: Oishi
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D016189:Dystrophin / Q000378:metabolism; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005455:Fluorescent Antibody Technique; D006652:Histological Techniques / Q000662:veterinary; D007564:Japan; D018482:Muscle, Skeletal / Q000473:pathology; D009137:Muscular Dystrophy, Animal / Q000175:diagnosis* / Q000473:pathology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012508:Sarcolemma / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000473:pathology*
chemical_list: D017931:DNA Primers; D016189:Dystrophin