title: Optimization and validation of high-performance liquid chromatography method for individual curcuminoids in turmeric by heat-refluxed extraction.
abstract: Turmeric is a plant in the Zingiberaceae family which contains curcuminoids as anticancer agents and has been widely used as a main ingredient in curry powder. However, there is a lack of suitable high performance liquid chromatography-diode array detection (HPLC-DAD) methods for reducing sample preparation time and peak resolution improvement of curcuminoids (bisdemethoxycurcumin, demethoxycurcumin, and curcumin). No significant differences in yield concentrations were observed after 60 min of heat-refluxed extraction (p < 0.05). Simultaneous chromatographic separation of all three curcuminoids achieved satisfactory results with a separation factor of 1.08 and a resolution factor of 3.39 with validation results in compliance with FDA guidelines. The expanded relative measurement uncertainty results 5.71-6.60 complied with CODEX draft. The method was successfully applied to the turmeric samples (n = 107, range 2.70-4.41 g/100 g, total curcuminoids 3.58 g/100 g). These results show that heat-refluxed extraction can be carried out easily with excellent precision and accuracy of total curcuminoids in turmeric samples.
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University , Seoul 151-921, Republic of Korea.
authors.forename: Young-Jun
authors.initials: YJ
authors.lastname: Kim
authors.forename: Hyong Joo
authors.initials: HJ
authors.lastname: Lee
authors.forename: Youngjae
authors.initials: Y
authors.lastname: Shin
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D030024:Curcuma / Q000737:chemistry*; D006358:Hot Temperature; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D010936:Plant Extracts