title: Pseudoislet of hybrid cellular spheroids from commercial cell lines.
abstract: Investigators conducting diabetes-related research have focused on islet transplantation as a radical therapy for type 1 diabetes mellitus. Pancreatic islet isolation, an essential process, is a very demanding work because of the proteolytic enzymes, species, treatment time, and individual difference. Replacement of primary isolated pancreatic islets must be carried out continuously for various in vitro tests, making primary isolated islets a useful tool for cell transplantation research. Hence, we sought to develop pseudoislets from commercial pancreas-derived cell lines. In this study, we used RIN-5F and RIN-m cells, which secrete insulin, somatostatin, or glucagon. To manufacture hybrid cellular spheroids, the cells were cultured under hanging drop plate and nonadhesive plate methods. We observed that hybrid cellular pseudoislets exhibited an oval shape, with sizes ranging from 590 to 1200 μm. Their morphology was similar to naïve islets. Cell line pseudoislets secreted and expressed insulin, glucagon, and somatostatin, as confirmed by reverse transcriptase polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemistry analyses. Thus, the current artificially manufactured biomimetic pseudoislets resembled pancreatic islets of the endocrine system, appearing as cellular aggregates that secreted insulin, glucagon, and somatostatin. Enhanced immunoisolation techniques may lead to the development of new islet sources for pancreatic transplantation through this pseudoislet strategy.
authors.affiliation: Regenerative Medicine Laboratory, Center for Stem Cell Research, Department of Biomedical Science and Technology (IBST), Konkuk University, Seoul, Republic of Korea.
authors.forename: Y H
authors.initials: YH
authors.lastname: Jo
authors.forename: B M
authors.initials: BM
authors.lastname: Nam
authors.forename: B Y
authors.initials: BY
authors.lastname: Kim
authors.forename: J G
authors.initials: JG
authors.lastname: Nemeno
authors.forename: S
authors.initials: S
authors.lastname: Lee
authors.forename: J E
authors.initials: JE
authors.lastname: Yeo
authors.forename: W
authors.initials: W
authors.lastname: Yang
authors.forename: S H
authors.initials: SH
authors.lastname: Park
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
authors.forename: J I
authors.initials: JI
authors.lastname: Lee
mesh_terms: D000818:Animals; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018874:Spheroids, Cellular / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes