title: Vitamin D status of children with severe early childhood caries: a case-control study.
abstract: BACKGROUND
  Severe Early Childhood Caries (S-ECC) affects the health and well-being of young children. There is limited research in this area, though evidence suggests that children with S-ECC are at an increased risk of malnutrition. The purpose of this study was to determine the association between vitamin D (25(OH)D) levels and S-ECC.
  
  
  METHODS
  This case-control study was conducted from 2009 to 2011 in the city of Winnipeg, Manitoba, Canada. 144 preschool children with S-ECC were recruited from a local health centre on the day of their slated dental surgery under general anesthetic. 122 caries-free controls were recruited from the community. Children underwent a blood draw for vitamin D (25(OH)D), calcium, parathyroid hormone, and albumin levels. Parents completed an interviewed questionnaire assessing the child's nutritional habits, oral health, and family demographics. Analyses included descriptive and bivariate statistics as well as multiple and logistic regression. A p value  ≤  0.05 was significant.
  
  
  RESULTS
  The mean age of participants was 40.8 ± 14.1 months. Children with S-ECC had significantly lower mean 25(OH)D (68.9 ± 28.0 nmol/L vs. 82.9 ± 31.1, p < 0.001), calcium (p < 0.001), and albumin (p < 0.001) levels, and significantly higher parathyroid hormone (p < 0.001) levels than those caries-free. Children with S-ECC were significantly more likely to have vitamin D levels below recognized thresholds for optimal and adequate status (i.e. < 75 and < 50 nmol/L, respectively). Multiple regression analysis revealed that S-ECC, infrequent milk consumption, and winter season were significantly associated with lower 25(OH)D concentrations. Low 25(OH)D levels, low household income, and poorer ratings of the child's general health were significantly associated with S-ECC on logistic regression.
  
  
  CONCLUSIONS
  Children with S-ECC appear to have relatively poor nutritional health compared to caries-free controls, and were significantly more likely to have low vitamin D, calcium, and albumin concentrations and elevated PTH levels.
authors.affiliation: The University of Manitoba, Winnipeg, Canada. umschrot@cc.umanitoba.ca.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Schroth
authors.forename: Jeremy A
authors.initials: JA
authors.lastname: Levi
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Sellers
authors.forename: James
authors.initials: J
authors.lastname: Friel
authors.forename: Eleonore
authors.initials: E
authors.lastname: Kliewer
authors.forename: Michael E K
authors.initials: ME
authors.lastname: Moffatt
mesh_terms: D017668:Age of Onset; D016022:Case-Control Studies; D002675:Child, Preschool; D015897:Comorbidity; D003430:Cross-Sectional Studies; D003731:Dental Caries / Q000097:blood* / Q000453:epidemiology / Q000209:etiology; D004032:Diet; D005260:Female; D006801:Humans; D006961:Hyperparathyroidism / Q000453:epidemiology; D034141:Hypoalbuminemia / Q000453:epidemiology; D008297:Male; D044342:Malnutrition / Q000453:epidemiology; D008350:Manitoba / Q000453:epidemiology; D009752:Nutritional Status; D010281:Parathyroid Hormone / Q000097:blood; D011788:Quality of Life; D012709:Serum Albumin / Q000032:analysis; D014070:Tooth / Q000254:growth & development; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000097:blood; D014808:Vitamin D Deficiency / Q000150:complications / Q000453:epidemiology*
chemical_list: D010281:Parathyroid Hormone; D012709:Serum Albumin; D014807:Vitamin D; C104450:25-hydroxyvitamin D