title: Regeneration of periodontal tissues using allogeneic periodontal ligament stem cells in an ovine model.
abstract: OBJECTIVE
  To investigate the capacity of allogeneic periodontal ligament stem cells (PDLSCs) to regenerate periodontal tissues using an ovine periodontal defect model.
  
  
  METHODS
  Surgically created zero-wall dehiscence periodontal defects created in Merino sheep were filled with 1 × 10(7) allogeneic PDLSCs attached to Gelfoam(®), Gelfoam alone or left untreated. After 4 weeks, histological analysis was performed to assess periodontal regeneration.
  
  
  RESULTS
  Allogeneic PDLSCs were well tolerated by recipient animals. The mean area of new alveolar bone was significantly greater in the PDLSC + Gelfoam treatment group compared with the defect-alone group. The PDLSC + Gelfoam and Gelfoam-only treatment groups displayed significantly greater length of new cementum and percentage of cementum regrowth compared with the defect-alone group. New Sharpey's fibers were generally more organized and significantly thicker within the PDLSC + Gelfoam treatment group. The PDLSC + Gelfoam treatment group also showed a trend of increased Sharpey's fiber attachment length compared with the Gelfoam-only and defect-alone groups.
  
  
  CONCLUSIONS
  These studies support the potential use of allogeneic PDLSC preparations as viable therapies for periodontal regeneration in the clinical setting.
authors.affiliation: School of Dentistry, Colgate Australian Clinical Dental Research Centre, University of Adelaide, Adelaide, SA, Australia.
authors.forename: Krzysztof Marek
authors.initials: KM
authors.lastname: Mrozik
authors.forename: Naohisa
authors.initials: N
authors.lastname: Wada
authors.forename: Victor
authors.initials: V
authors.lastname: Marino
authors.forename: Ward
authors.initials: W
authors.lastname: Richter
authors.forename: Songtao
authors.initials: S
authors.lastname: Shi
authors.forename: Donna L
authors.initials: DL
authors.lastname: Wheeler
authors.forename: Stan
authors.initials: S
authors.lastname: Gronthos
authors.forename: P Mark
authors.initials: PM
authors.lastname: Bartold
mesh_terms: D000539:Alveolar Process / Q000254:growth & development; D000818:Animals; D003739:Dental Cementum / Q000502:physiology; D004195:Disease Models, Animal; D005260:Female; D020007:Implants, Experimental; D010012:Osteogenesis; D010513:Periodontal Ligament / Q000166:cytology* / Q000502:physiology*; D012038:Regeneration / Q000502:physiology*; D012756:Sheep; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D014184:Transplantation, Homologous