title: Aerobic production of succinate from arabinose by metabolically engineered Corynebacterium glutamicum.
abstract: Arabinose is considered as an ideal feedstock for the microbial production of value-added chemicals due to its abundance in hemicellulosic wastes. In this study, the araBAD operon from Escherichia coli was introduced into succinate-producing Corynebacterium glutamicum, which enabled aerobic production of succinate using arabinose as sole carbon source. The engineered strain ZX1 (pXaraBAD, pEacsAgltA) produced 74.4 mM succinate with a yield of 0.58 mol (mol arabinose)(-1), which represented 69.9% of the theoretically maximal yield. Moreover, this strain produced 110.2 mM succinate using combined substrates of glucose and arabinose. To date, this is the highest succinate production under aerobic conditions in minimal medium.
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, People's Republic of China; Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People's Republic of China. Electronic address: chentao@tju.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Nianqing
authors.initials: N
authors.lastname: Zhu
authors.forename: Huihua
authors.initials: H
authors.lastname: Xia
mesh_terms: D000332:Aerobiosis / Q000187:drug effects; D001089:Arabinose / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D048230:Corynebacterium glutamicum / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D060847:Metabolic Engineering / Q000379:methods*; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D007544:Isopropyl Thiogalactoside; D019802:Succinic Acid; D001089:Arabinose; D005947:Glucose
keywords: Acetyl-CoA synthetase; Arabinose; Citrate synthetase; Metabolic engineering; araD Operon