title: The role of adiponectin in secondary inflammatory reaction in cerebral ischemia.
abstract: OBJECTIVE
  In this study, we investigate the role of adiponectin in the interaction between leukocytes and endothelium in the secondary inflammatory reaction of cerebral ischemia.
  
  
  METHODS
  Adiponectin knock-out mice group (APN-KO) (n = 8) and wild-type mice group (WT) (n = 8) were prepared. Each group was sub-divided into 2 groups by reperfusion time. One-hour middle cerebral artery occlusion and reperfusion were induced using the intraluminal filament technique. At 6 and 12 hours after the occlusion, the mice were placed on a stereotactic frame to perform craniotomy in the left parietal area. After craniotomy, a straight pial venule was selected as a target vessel. With the fluorescence intravital microscope, the number of rolling leukocytes and leukocytes that adhered to endothelium were counted and documented at 6 and 12 hours after the reperfusion.
  
  
  RESULTS
  At 6 and 12 hours after the reperfusion, more rolling leukocyte and leukocyte adhesion were observed in the APN-KO mice than in the WT mice. The difference in leukocyte numbers between the APN-KO and WT mice was found to be statistically significant (p = 0.029) by Mann-Whitney U-test.
  
  
  CONCLUSIONS
  We found that adiponectin inhibits the interaction between the endothelium and leukocytes in cerebral ischemia-reperfusion. Therefore adiponectin might prevent the secondary insult caused by the inflammation reaction.
authors.affiliation: Department of Neurosurgery, Korea University Ansan Hospital, School of Medicine, Korea University, Ansan, Korea.
authors.forename: Yong-Su
authors.initials: YS
authors.lastname: Jung
authors.forename: Sung-Kon
authors.initials: SK
authors.lastname: Ha
authors.forename: Sang-Dae
authors.initials: SD
authors.lastname: Kim
authors.forename: Se-Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: Dong-Jun
authors.initials: DJ
authors.lastname: Lim
authors.forename: Jong-Il
authors.initials: JI
authors.lastname: Choi
keywords: Adiponectin; Cerebral ischemia; MCAO-R; Secondary inflammatory reaction