title: Elevated expression of pleiotrophin in pilocarpine-induced seizures of immature rats and in pentylenetetrazole-induced hippocampal astrocytes in vitro.
abstract: Pleiotrophin (PTN) is a secreted extracellular matrix (ECM)-associated cytokine that has emerged as an important neuromodulator with multiple neuronal functions. In the present study, we detected and compared the dynamic expression of PTN in the hippocampus and adjacent cortex of immature rats with pilocarpine-induced epilepsy. Moreover, we also confirmed the results by examining PTN expression in hippocampal astrocytes cultured in the presence of pentylenetetrazole (PTZ). Immunohistochemistry showed faint immunostaining of PTN in the control hippocampus and adjacent cortex. Notably, PTN immunoreactivity began to increase in relatively small cells in the hippocampus and adjacent cortex at 2h and 3 weeks after seizures, and the labeling intensity reached the maximum level in the hippocampus and adjacent cortex at 8 weeks after seizures. Furthermore, we also found that PTZ treatment significantly reduced astrocytic viability in a dose-dependent manner and time-dependently increased expression levels of PTN in hippocampal astrocytes. In conclusion, our data suggest that increased expression of PTN in the brain tissues may be involved in epileptogenesis.
authors.affiliation: Department of Pediatrics, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, People's Republic of China; Department of Pediatrics, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, People's Republic of China.
authors.forename: Shuqin
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Le
authors.affiliation: Department of Pediatrics, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, People's Republic of China. Electronic address: cmu2hwanghua@163.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D010433:Pentylenetetrazole / Q000494:pharmacology*; D010862:Pilocarpine; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced*
chemical_list: D002352:Carrier Proteins; D016207:Cytokines; D010862:Pilocarpine; C066845:pleiotrophin; D010433:Pentylenetetrazole
keywords: Astrocyte; Epilepsy; Lithium–pilocarpine; Pentylenetetrazole; Pleiotrophin; Rats