title: SIVSM/HIV-2 Vpx proteins: function and uses in the infection of primary myeloid cells.
abstract: Vpx is coded almost exclusively by members of the SIVSM/HIV-2 lineage of primate lentiviruses, it is incorporated into virion particles and is thus present during the early phases of infection of target cells. While Vpx exerts no detectable function during the infection of most cell types, it potently counteracts a cellular restriction that targets incoming lentiviruses specifically in myeloid cells. As a consequence of this function, Vpx improves the efficiency of lentiviral infection of dendritic cells (DCs), macrophages, and monocytes. Here, we describe how the positive function exerted by Vpx during the early phases of infection of myeloid cells can be used to augment the efficiency of lentiviral vector-mediated gene transfer in these cells.
authors.affiliation: Department of Human Virology, INSERM 758, IFR128, ENS Lyon, Université de Lyon I, Lyon, France.
authors.forename: Gregory
authors.initials: G
authors.lastname: Berger
authors.forename: Andrea
authors.initials: A
authors.lastname: Cimarelli
mesh_terms: D003713:Dendritic Cells / Q000166:cytology* / Q000821:virology*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D057809:HEK293 Cells; D015498:HIV-2 / Q000378:metabolism / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D009000:Monocytes / Q000166:cytology*; D015302:Simian Immunodeficiency Virus / Q000235:genetics*; D054334:Viral Regulatory and Accessory Proteins / Q000378:metabolism*
chemical_list: C074286:VPX protein, Human immunodeficiency virus 2; D054334:Viral Regulatory and Accessory Proteins