title: Preparation of hollow nickel silicate nanospheres for separation of His-tagged proteins.
abstract: Hollow nickel silicate nanospheres (NiSiO3 NSs) with hierarchical shells were hydrothermally synthesized by using silica spheres as a template. The NiSiO3 NSs have an average diameter of 250 nm with a shell thickness of 50 nm, and the hierarchical shell consists of a large number of sheets. By taking advantage of the high affinity of Ni(2+) toward histidine-tagged (His-tagged) proteins, hollow NiSiO3 NSs can be used to enrich and separate His-tagged proteins directly from a mixture of lysed cells. Results indicated that the hollow NiSiO3 NSs presented negligible nonspecific protein adsorption and a high protein binding ability with a high binding capacity of 13.2 mmol g(-1). Their specificity and affinity toward His-tagged proteins remained after recycling 5 times. The hollow NiSiO3 NSs are especially suitable for rapid purification of His-tagged proteins.
authors.affiliation: Key Laboratory for Special Functional Materials, Henan University, Kaifeng 475004, P. R. China. zhaoyb902@henu.edu.cn.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Wu
authors.forename: Guanxiao
authors.initials: G
authors.lastname: Chang
authors.forename: Yanbao
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D006639:Histidine / Q000737:chemistry*; D054118:Nanospheres / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D009532:Nickel / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D017640:Silicates / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D011506:Proteins; D017640:Silicates; D006639:Histidine; D012822:Silicon Dioxide; D009532:Nickel