title: An unusual polymorphic locus useful for tagging Rps1 resistance alleles in soybean.
abstract: The Phytophthora root and stem resistance locus Rps1 has been mapped to linkage group N of the USDA-ARS soybean molecular map, approximately 2 cM from locus A071-1. To determine if A071-1 polymorphisms exist that distinguish and tag different Rps1 alleles, germplasms containing the seven Rps1 alleles were screened with eight enzymes for pA071-detectable polymorphisms. Six enzymes revealed at least one polymorphic fragment. All six detected a polymorphism at A071-1 as determined by restriction fragment length polymorphism mapping, comparison to an EMBL3 clone containing locus A071-1, and Southern hybridization with probes specific for locus A071-1. Screening of the Rps1 donors and 24 Rps1-and 15 Rps1-containing U.S. soybean varieties showed that locus A071-1 exhibited three polymorphisms with each enzyme. The polymorphisms detected by one enyme did not always correlate with those detected by the other four, suggesting that multiple mutation events may be responsible for the different A071-1 polymorphisms. Although no combination of alleles distinguished Rps1-and Rps1-containing genotypes, polymorphism at A071-1 made it possible to distinguish five groups of soybean germplasms. Thus, the unusual polymorphism of locus A071-1 should useful for following Rps1 inheritance in many breeding programs.
authors.affiliation: USDA-ARS Field Crops Research, Agronomy Hall, Iowa State University, 50011, Ames, IA, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Polzin
authors.forename: L L
authors.initials: LL
authors.lastname: Lorenzen
authors.forename: T C
authors.initials: TC
authors.lastname: Olson
authors.forename: R C
authors.initials: RC
authors.lastname: Shoemaker