title: Skin and plasma autofluorescence during hemodialysis: a pilot study.
abstract: Skin autofluorescence (AF) is related to the accumulation of advanced glycation end products (AGEs) and is one of the strongest prognostic markers of mortality in hemodialysis (HD) patients. The aim of this pilot study was to investigate whether changes in skin AF appear after a single HD session and if they might be related to changes in plasma AF. Skin and plasma AF were measured before and after HD in 35 patients on maintenance HD therapy (nine women and 26 men, median age 68 years, range 33-83). Median dialysis time was 4 h (range 3-5.5). Skin AF was measured noninvasively with an AGE Reader, and plasma AF was measured before and after HD at 460 nm after excitation at 370 nm. The HD patients had on average a 65% higher skin AF value than age-matched healthy persons (P < 0.001). Plasma AF was reduced by 14% (P < 0.001), whereas skin AF was not changed after a single HD treatment. No significant influence of the reduced plasma AF on skin AF levels was found. This suggests that the measurement of skin AF can be performed during the whole dialysis period and is not directly influenced by the changes in plasma AF during HD.
authors.affiliation: Department of UMC Staff, Universities of Groningen, Groningen, The Netherlands.
authors.forename: Reindert
authors.initials: R
authors.lastname: Graaff
authors.forename: Stefan
authors.initials: S
authors.lastname: Arsov
authors.forename: Bernd
authors.initials: B
authors.lastname: Ramsauer
authors.forename: Marten
authors.initials: M
authors.lastname: Koetsier
authors.forename: Nils
authors.initials: N
authors.lastname: Sundvall
authors.forename: Gerwin E
authors.initials: GE
authors.lastname: Engels
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Sikole
authors.forename: Lennart
authors.initials: L
authors.lastname: Lundberg
authors.forename: Gerhard
authors.initials: G
authors.lastname: Rakhorst
authors.forename: Bernd
authors.initials: B
authors.lastname: Stegmayr
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D017127:Glycation End Products, Advanced / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D006435:Renal Dialysis; D012867:Skin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D015415:Biomarkers; D017127:Glycation End Products, Advanced
keywords: Advanced glycation end products.; Hemodialysis; Plasma autofluorescence; Skin autofluorescence