title: The effect on proliferation and differentiation of cementoblast by using sclerostin as inhibitor.
abstract: Cementogenesis is of great importance for normal teeth root development and is involved in the repair process of root resorption caused by orthodontic treatment. As highly differentiated mesenchymal cells, cementoblasts are responsible for this process under the regulation of many endogenous agents. Among these molecules, sclerostin has been much investigated recently for its distinct antagonism effect on bone metabolism. Encoded by the sost gene, sclerostin is expressed in osteocytes and cementocytes of cellular cementum. it is still unclear. In the current study, we investigated the effects of sclerostin on the processes of proliferation and differentiation; a series of experiments including MTT, apoptosis examination, alkaline phosphatase (ALP) activity, gene analysis, and alizarin red staining were carried out to evaluate the proliferation and differentiation of cementoblasts. Protein expression including osteoprotegerin (OPG) and receptor activator of nuclear factor kappa B ligand (RANKL) were also checked to analyze changes in osteoclastogenesis. Results show that sclerostin inhibits cementoblasts proliferation and differentiation, and promotes osteoclastogenesis. Interestingly, the monoclonal antibody for sclerostin has shown positive effects on osteoporosis, indicating that it may facilitate cementogenesis and benefit the treatment of cementum related diseases.
authors.affiliation: Department of Orthodontics, School of Stomatology, Jilin University, Changchun 130021, China. humin@jlu.edu.cn.
authors.forename: Xingfu
authors.initials: X
authors.lastname: Bao
authors.forename: Yuyan
authors.initials: Y
authors.lastname: Liu
authors.forename: Guanghong
authors.initials: G
authors.lastname: Han
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zuo
authors.forename: Min
authors.initials: M
authors.lastname: Hu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D049109:Cell Proliferation; D025021:Cementogenesis / Q000502:physiology*; D003739:Dental Cementum / Q000378:metabolism* / Q000502:physiology*; D006023:Glycoproteins / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D053244:Osteoprotegerin / Q000378:metabolism; D053245:RANK Ligand / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D053244:Osteoprotegerin; D053245:RANK Ligand; C486030:Sost protein, mouse; D000469:Alkaline Phosphatase