title: Modified oligodeoxynucleotide primers for reverse-transcription of target RNAs that can discriminate among length variants at the 3'-terminus.
abstract: To discriminate among miRNA length variants, we synthesized conformationally restricted or unrestricted oligonucleotides containing a cyclohexyl phosphate residue. These oligonucleotides formed duplexes with length-matched complementary miRNAs more tightly than with length variants. The use of one of these modified oligodeoxynucleotides as a reverse transcription primer enabled a novel RT-PCR that can discriminate among miRNA length variants.
authors.affiliation: Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, Japan. msekine@bio.titech.ac.jp kseio@bio.titech.ac.jp.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Iijima
authors.forename: Shun
authors.initials: S
authors.lastname: Kojima
authors.forename: Erika
authors.initials: E
authors.lastname: Kodama
authors.forename: Sayako
authors.initials: S
authors.lastname: Kurohagi
authors.forename: Takashi
authors.initials: T
authors.lastname: Kanamori
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Masaki
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ohkubo
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sekine
authors.forename: Kohji
authors.initials: K
authors.lastname: Seio
mesh_terms: D017931:DNA Primers / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D048348:Reverse Transcription / Q000235:genetics*
chemical_list: D017931:DNA Primers; D035683:MicroRNAs