title: Stabilized Wittig olefination for bioconjugation.
abstract: Stabilized Wittig olefination holds great potential as a bioconjugation reaction. We demonstrate that the reaction of stabilized phosphorus ylides (or phosphonium salts) with aryl aldehydes is sufficiently robust to be used for live cell affinity isolation and fluorescence tagging of a protein, FKBP12.
authors.affiliation: Division of Organic Chemistry, Institute of Chemical and Engineering Sciences, Agency for Science Technology and Research Singapore, 11 Biopolis Way, Helios Block #03-08, Singapore. chan_kok_ping@ices.a-star.edu.sg.
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Lum
authors.forename: Vanessa J
authors.initials: VJ
authors.lastname: Xavier
authors.forename: Michelle J-H
authors.initials: MJ
authors.lastname: Ong
authors.forename: Charles W
authors.initials: CW
authors.lastname: Johannes
authors.forename: Kok-Ping
authors.initials: KP
authors.lastname: Chan
mesh_terms: D000447:Aldehydes / Q000737:chemistry; D000475:Alkenes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D010758:Phosphorus / Q000737:chemistry; D022061:Tacrolimus Binding Protein 1A / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000447:Aldehydes; D000475:Alkenes; D005456:Fluorescent Dyes; D010758:Phosphorus; D022061:Tacrolimus Binding Protein 1A