title: MiR-200a impairs glioma cell growth, migration, and invasion by targeting SIM2-s.
abstract: Recently, single-minded homolog 2-short form (SIM2-s) was reported to be related to tumor development and progression and to be elevated in many human cancer cells. In this study, we investigated the factors that contribute to the regulation of SIM2-s expression in gliomas. The results showed that SIM2-s was elevated in gliomas. In addition, inhibition of SIM2-s reduced glioma cell growth, migration, and invasion. Next, we demonstrated that SIM2-s is a functional target of miR-200a. Further, miR-200a is downregulated in human glioma and inhibition of miR-200a caused upregulation of SIM2-s in T98G cells and promoted their motility. Finally, blockage of miR-200a expression in a mouse model of human glioma resulted in significant promotion of tumor growth. These findings suggest that miR-200a could serve as a therapeutic tool for glioma.
authors.affiliation: Departments of aEmergency Surgery bNeurosurgery, Qilu Hospital of Shandong University cBrain Science Research Institute, Shandong University, Jinan dDepartment of Neurology, The Affiliated Yantai Yuhuangding Hospital of Qingdao Medical College, Yantai, China.
authors.forename: Yuhang
authors.initials: Y
authors.lastname: Su
authors.forename: Qiaowei
authors.initials: Q
authors.lastname: He
authors.forename: Lin
authors.initials: L
authors.lastname: Deng
authors.forename: Juntao
authors.initials: J
authors.lastname: Wang
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Liu
authors.forename: Donghai
authors.initials: D
authors.lastname: Wang
authors.forename: Qibing
authors.initials: Q
authors.lastname: Huang
authors.forename: Gang
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D035683:MicroRNAs; C533896:MIRN200 microRNA, human; C417032:SIM2 protein, human