title: The expression and regulation of DFNA5 in human hepatocellular carcinoma DFNA5 in hepatocellular carcinoma.
abstract: Hepatocellular carcinoma is a primary malignancy of hepatocytes which accounts for 80 % of all primary liver cancers. DFNA5 has been identified as a tumor suppressor gene with an important role in several frequent forms of cancers, while little is known about its role in hepatocellular carcinoma. Through comparison of the DFNA5 protein expression in hepatocellular carcinoma cells (HepG2) with human fetal lung fibroblast cells (MRC5), we found that the DFNA5 protein expression in hepatocellular carcinoma cells was significantly lower than that in normal cells. The transfection of DFNA5 gene into HepG2 cells could increase DFNA5 protein expression, which subsequently led to inhibition of cell proliferation. Underlying mechanism study revealed that decreased proliferation was due to increased apoptosis and cell cycle arrest. In view of the important role of DFNA5 gene in carcinogenesis, these findings are expected to provide new understanding on development and treatment of human hepatocellular carcinoma.
authors.affiliation: Department of General Surgery, Shanghai East Hospital, Tongji University School of Medicine, No 150 Jimo Road, Pudong New Area, Shanghai, 200120, China.
authors.forename: Cong-Jun
authors.initials: CJ
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Tang
authors.forename: Dong-Wei
authors.initials: DW
authors.lastname: Shen
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Qiong-Ying
authors.initials: QY
authors.lastname: Yuan
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.forename: Yong-Kun
authors.initials: YK
authors.lastname: Wang
authors.forename: Rong-Hua
authors.initials: RH
authors.lastname: Xu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D011960:Receptors, Estrogen / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D000094524:Gasdermins
chemical_list: D051017:Apoptosis Regulatory Proteins; D011960:Receptors, Estrogen; C111432:GSDME protein, human; D000094524:Gasdermins