title: Fluorescent probes that distinguish proteins with single or two close mercapto groups.
abstract: Two water soluble fluorescent probes with quaternary ammonium salts as solubilization groups were designed and synthesized to detect proteins with close mercapto groups. The unique structure of V-shape oligo(o-phenylene-ethynylene) backbone connecting two maleimide groups as mercapto group recognition sites enables the probes to distinguish a single mercapto group from two close mercapto groups in proteins. The probe had no fluorescence response to proteins with only one mercapto group, but had strong fluorescence response to proteins with mercapto group combination at the concentration of 10(-6) mol/L. The probe worked under a wide range of pH, from 4 to 8. This work also demonstrates the concept of functional group combination detection.
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), Key Lab of Polymer Chemistry & Physics of Ministry of Education, College of Chemistry, Peking University, Beijing 100871, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Gao
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Shu
authors.initials: S
authors.lastname: Xu
authors.forename: Yi
authors.initials: Y
authors.lastname: Cheng
authors.forename: Yuguo
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000480:Alkynes / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008301:Maleimides / Q000737:chemistry; D009842:Oligopeptides / Q000032:analysis* / Q000138:chemical synthesis; D000644:Quaternary Ammonium Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence
chemical_list: D000480:Alkynes; D005456:Fluorescent Dyes; D008301:Maleimides; D009842:Oligopeptides; D000644:Quaternary Ammonium Compounds; D012996:Solutions; C508010:o-phenylene ethynylene
keywords: Fluorescent probe; Maleimide; Mercapto group detection; Protein detection