title: Delineation of the TRAK binding regions of the kinesin-1 motor proteins.
abstract: Understanding specific cargo distribution in differentiated cells is a major challenge. Trafficking kinesin proteins (TRAKs) are kinesin adaptors. They bind the cargo binding domain of kinesin-1 motor proteins forming a link between the motor and their cargoes. To refine the TRAK1/2 binding sites within the kinesin-1 cargo domain, rationally designed C-terminal truncations of KIF5A and KIF5C were generated and their co-association with TRAK1/2 determined by quantitative co-immunoprecipitations following co-expression in mammalian cells. Three contributory regions forming the TRAK2 binding site within KIF5A and KIF5C cargo binding domains were delineated. Differences were found between TRAK1/2 with respect to association with KIF5A.
authors.affiliation: University College London School of Pharmacy, 29/39 Brunswick Square, London WC1N 1AX, United Kingdom.
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Randall
authors.forename: Carolyn
authors.initials: C
authors.lastname: Moores
authors.forename: F Anne
authors.initials: FA
authors.lastname: Stephenson
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D057809:HEK293 Cells; D006801:Humans; D016547:Kinesins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D051381:Rats
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; C500506:KIF5A protein, human; C531552:TRAK1 protein, human; C514435:KIF5C protein, human; D016547:Kinesins
keywords: FEZ1; FRET; Forster resonance energy transfer; GRIP; HAP1; HEK; Huntingtin-associated protein; Intracellular transport; Kinesin; Kinesin adaptor protein; MADD; Mitochondrial trafficking; Motor protein; TRAK; adaptor protein mitogen-activated protein kinase-activating death domain; fasciculation and elongation protein-ζ; glutamate receptor-interacting protein 1; human embryonic kidney; trafficking kinesin protein