title: [Serological diagnosis of HTLV-1/2: combination of screening assays to define the serological status in blood donors].
abstract: Alternative algorithms were evaluated in order to reduce the number of false reactive results for antibodies against HTLV-1/2. From 20,210 samples tested, 0.37% (74/20,210) was reactive by ELISA Murex. Of these, 23 were confirmed as positive by the indirect immunofluorescence assay whereas 51 were negative, being the positive predictive value (PPV) 31.08%. From a combination of the ELISA Murex assay with the particle agglutination assay (PA) and ELISA MP, the following results were obtained: 26/74 were reactive by ELISA Murex and PA, PPV 88.5% and 32/74 were reactive by ELISA Murex and ELISA MP, PPV 71.8 %. The ROC curve analysis determined that for an RP 4.74, the values for sensitivity, specificity, PPV and NPV by ELISA Murex were 100%, 98.04%, 95.8% and 100%, respectively. We propose that reactive samples by ELISA Murex with an RP d 4.74 should be retested in duplicate by PA, and the resulting concordantly nonreactive samples should be defined as negative for HTLV-1/2.
authors.affiliation: Departamento Laboratorio Central, Ministerio de Salud de la Provincia de Córdoba, Córdoba, Argentina.
authors.forename: Claudia
authors.initials: C
authors.lastname: Moreno
authors.forename: Marcos
authors.initials: M
authors.lastname: Balangero
authors.forename: María G
authors.initials: MG
authors.lastname: Barbás
authors.forename: Analía
authors.initials: A
authors.lastname: Cudolá
authors.forename: Sandra
authors.initials: S
authors.lastname: Gallego
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D001782:Blood Donors; D046148:Donor Selection / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D015490:HTLV-I Infections / Q000097:blood* / Q000175:diagnosis*; D015491:HTLV-II Infections / Q000097:blood* / Q000175:diagnosis*; D015368:Human T-lymphotropic virus 1 / Q000276:immunology*; D015367:Human T-lymphotropic virus 2 / Q000276:immunology*; D006801:Humans; D012698:Serologic Tests
chemical_list: D000914:Antibodies, Viral