title: Transcriptional response to deletion of the phosphatidylserine decarboxylase Psd1p in the yeast Saccharomyces cerevisiae.
abstract: In the yeast, Saccharomyces cerevisiae, the synthesis of the essential phospholipid phosphatidylethanolamine (PE) is accomplished by a network of reactions which comprises four different pathways. The enzyme contributing most to PE formation is the mitochondrial phosphatidylserine decarboxylase 1 (Psd1p) which catalyzes conversion of phosphatidylserine (PS) to PE. To study the genome wide effect of an unbalanced cellular and mitochondrial PE level and in particular the contribution of Psd1p to this depletion we performed a DNA microarray analysis with a ∆psd1 deletion mutant. This approach revealed that 54 yeast genes were significantly up-regulated in the absence of PSD1 compared to wild type. Surprisingly, marked down-regulation of genes was not observed. A number of different cellular processes in different subcellular compartments were affected in a ∆psd1 mutant. Deletion mutants bearing defects in all 54 candidate genes, respectively, were analyzed for their growth phenotype and their phospholipid profile. Only three mutants, namely ∆gpm2, ∆gph1 and ∆rsb1, were affected in one of these parameters. The possible link of these mutations to PE deficiency and PSD1 deletion is discussed.
authors.affiliation: Institute of Biochemistry, Graz University of Technology, Graz, Austria.
authors.forename: Martina
authors.initials: M
authors.lastname: Gsell
authors.forename: Gerald
authors.initials: G
authors.lastname: Mascher
authors.forename: Irmgard
authors.initials: I
authors.lastname: Schuiki
authors.forename: Birgit
authors.initials: B
authors.lastname: Ploier
authors.forename: Claudia
authors.initials: C
authors.lastname: Hrastnik
authors.forename: Günther
authors.initials: G
authors.lastname: Daum
mesh_terms: D002262:Carboxy-Lyases / Q000235:genetics* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D042541:Intracellular Space; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D016366:Open Reading Frames; D010641:Phenotype; D010743:Phospholipids / Q000378:metabolism; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D024101:Mitochondrial Proteins; D010743:Phospholipids; D029701:Saccharomyces cerevisiae Proteins; D002262:Carboxy-Lyases; C577156:Psd1 protein, S cerevisiae