title: Natural killer cell cytotoxicity, cytokine and neuroendocrine responses to opioid receptor blockade during prolonged restraint in pigs.
abstract: This study evaluated porcine natural killer cell cytotoxicity (NKCC), plasma cytokines including interleukin (IL) 1β, IL-6, IL-10, IL-12 and tumor necrosis factor-α and plasma stress-related hormones including prolactin (PRL), growth hormone (GH), β-endorphin (BEND), ACTH and cortisol (COR) during a 4h restraint and recovery phase after saline or naloxone (1mg/kg BW) administration. The restraint preceded with saline altered NKCC and IL-12 concentration (an early from 15 to 60 min increase followed by a decrease) and increased other measured cytokines and hormones concentrations. Naloxone pretreatment blocked the suppressive effects of the restraint on NKCC and IL-12 and altered IL-10, IL-6, TNF-α, PRL and ACTH concentrations. Furthermore, in naloxone-injected pigs, a positive correlation was found between NKCC and all measured cytokines (with the exception of IL-6) and BEND, ACTH and COR. Results suggest that naloxone-sensitive opioid pathways could influence the mechanisms underlying the immune system (including NKCC) response during stress.
authors.affiliation: Department of Animal and Human Physiology, University of Gdańsk, Gdańsk, Poland. Electronic address: zetciep@gazeta.pl.
authors.forename: Z M
authors.initials: ZM
authors.lastname: Ciepielewski
authors.forename: W
authors.initials: W
authors.lastname: Stojek
authors.forename: A
authors.initials: A
authors.lastname: Borman
authors.forename: D
authors.initials: D
authors.lastname: Myślińska
authors.forename: W
authors.initials: W
authors.lastname: Glac
authors.forename: M
authors.initials: M
authors.lastname: Kamyczek
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000097:blood / Q000502:physiology; D000818:Animals; D016207:Cytokines / Q000097:blood / Q000502:physiology*; D013006:Growth Hormone / Q000097:blood / Q000502:physiology; D006854:Hydrocortisone / Q000097:blood / Q000502:physiology; D016753:Interleukin-10 / Q000097:blood / Q000502:physiology; D018664:Interleukin-12 / Q000097:blood / Q000502:physiology; D053583:Interleukin-1beta / Q000097:blood / Q000502:physiology; D015850:Interleukin-6 / Q000097:blood / Q000502:physiology; D007694:Killer Cells, Natural / Q000502:physiology*; D008297:Male; D009270:Naloxone / Q000494:pharmacology*; D009292:Narcotic Antagonists; D011388:Prolactin / Q000097:blood / Q000502:physiology; D012149:Restraint, Physical / Q000502:physiology / Q000662:veterinary*; D013312:Stress, Physiological / Q000502:physiology; D013552:Swine / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000502:physiology; D001615:beta-Endorphin / Q000097:blood / Q000502:physiology
chemical_list: D016207:Cytokines; D053583:Interleukin-1beta; D015850:Interleukin-6; D009292:Narcotic Antagonists; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D018664:Interleukin-12; D009270:Naloxone; D001615:beta-Endorphin; D000324:Adrenocorticotropic Hormone; D011388:Prolactin; D013006:Growth Hormone; D006854:Hydrocortisone
keywords: Acute prolonged stress; Endogenous opioid peptides; Naloxone; Natural killer cell activity; Pig; Restraint