title: Influence of EDTA and dentine in tissue dissolution ability of sodium hypochlorite.
abstract: This study verified whether ethylenediaminetetraacetic acid (EDTA) influences the pulp tissue dissolution capability of different concentrations of NaOCl, in the presence of dentine. NaOCl and EDTA solutions were simultaneously mixed in flasks either containing a dentine disc or those not containing a dentine disc. Previously weighed bovine pulp tissues were immersed in the solutions for 5, 15 and 30 min. The weight loss was measured. The dissolution tests were performed in triplicate. Univariate analysis of variance, along with further Tukey's honestly significant difference pairwise comparisons, was used to verify the effect of EDTA, different concentrations of NaOCl, dentine and time of incubation on the tissue dissolution. Higher concentrations of NaOCl increased the tissue dissolution. EDTA reduced the capacity of NaOCl to dissolve pulp tissue, even in presence of dentine. Dentine negatively affects the capacity of NaOCl to dissolve pulp tissue. In conclusion, the presence of EDTA and dentine negatively affects the tissue dissolution ability of NaOCl.
authors.affiliation: Department of Semiology and Clinics, Faculty of Dentistry, Federal University of Pelotas, Pelotas, Brazil.
authors.forename: Luiza Helena Silva
authors.initials: LH
authors.lastname: de Almeida
authors.forename: Natália Gomes e Silva
authors.initials: NG
authors.lastname: Leonardo
authors.forename: Ana Paula Neutzling
authors.initials: AP
authors.lastname: Gomes
authors.forename: Erick Miranda
authors.initials: EM
authors.lastname: Souza
authors.forename: Fernanda Geraldes
authors.initials: FG
authors.lastname: Pappen
mesh_terms: D000818:Animals; D002417:Cattle; D003782:Dental Pulp / Q000187:drug effects*; D003804:Dentin / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology*; D006801:Humans; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D012995:Solubility
chemical_list: D004492:Edetic Acid; D012973:Sodium Hypochlorite
keywords: EDTA; dentine; sodium hypochlorite; tissue dissolution