title: [Anti-human 4-1BBL monoclonal antibody stimulates the nuclear translocation of NF-κB and the co-location of 4-1BBL/CD28 isoform in U937 cells].
abstract: This study was purposed to investigate the molecular mechanism of 4-1BBL reverse signals in the human acute monocytic leukemia cell line of U937. The U937 cell line was used as target cells, and stimulated by the mouse anti-human 4-1BBL monoclonal antibody 1F1. The nuclear translocation of NF-κB and the co-location of 4-1BBL and CD28i molecules in U937 cells were observed with confocal laser scanning microscopy. The protein and m-RNA expression levels of 4-1BBL and CD28i were detected by flow cytometry and RT-PCR respectively. The results showed that the significant nuclear translocation of NF-κB and co-localization of 4-1BBL and CD28i on membrane of U937 cells appeared after being stimulated by mAb1F1. It is concluded that the 4-1BBL reverse signals transduction mediating the growth of U937 cells relates with the nuclear translocation of NF-κB. CD28i may be involved in intracellular 4-1BBL reverse signaling pathways.
authors.affiliation: Department of Hematology, Department of Geriatrics Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing 210029, Jiangsu Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Qiu
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: Chen
authors.forename: Ju-Juan
authors.initials: JJ
authors.lastname: Wang
authors.forename: Xiang-Chou
authors.initials: XC
authors.lastname: Yang
authors.forename: Ji
authors.initials: J
authors.lastname: Xu
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Duan
mesh_terms: D053330:4-1BB Ligand / Q000276:immunology / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D018106:CD28 Antigens / Q000378:metabolism; D018920:Coculture Techniques; D006801:Humans; D016328:NF-kappa B / Q000235:genetics*; D015398:Signal Transduction; D020298:U937 Cells
chemical_list: D053330:4-1BB Ligand; D000911:Antibodies, Monoclonal; D018106:CD28 Antigens; D016328:NF-kappa B