title: DART-MS for rapid, preliminary screening of urine for DMAA.
abstract: Dimethylamylamine (DMAA) is a sympathomimetic amine found in weight-loss/workout supplements or used as an appetite suppressant. DMAA is a stimulant that is banned by the World Anti-Doping Agency (WADA). Adverse health effects as well as fatalities have been implicated with its use. Direct analysis in real time mass spectrometry (DART-MS) is an ambient ionization method that was employed to rapidly identify the presence of DMAA in various samples without any extraction or preparations whatsoever. DMAA was first identified in supplements, sampled directly in their solid forms. Furthermore, DMAA was detected directly in urine over 48 h as a means of indicating recent abuse of the substance. DART-MS analysis is instantaneous, and coupled with the high mass accuracy associated with the time-of-flight mass analyzer, results in unequivocal identification of the presence of DMAA. These features demonstrate DART-MS as an attractive potential alternative screening method for the presence of drugs and medications or for toxicological investigations.
authors.affiliation: Department of Chemistry, University at Albany, State University of New York (SUNY), 1400 Washington Ave, Albany, NY, 12222, USA.
authors.forename: Ashton D
authors.initials: AD
authors.lastname: Lesiak
authors.forename: Kendra J
authors.initials: KJ
authors.lastname: Adams
authors.forename: Marek A
authors.initials: MA
authors.lastname: Domin
authors.forename: Colin
authors.initials: C
authors.lastname: Henck
authors.forename: Jason R E
authors.initials: JR
authors.lastname: Shepard
mesh_terms: D000588:Amines / Q000652:urine*; D001067:Appetite Depressants / Q000493:pharmacokinetics; D019587:Dietary Supplements / Q000032:analysis*; D004300:Doping in Sports; D006801:Humans; D013058:Mass Spectrometry / Q000191:economics / Q000379:methods*; D015813:Substance Abuse Detection / Q000191:economics / Q000379:methods; D013997:Time Factors
chemical_list: C571979:1,3-dimethylamylamine; D000588:Amines; D001067:Appetite Depressants
keywords: DART-MS; DMAA; collision induced dissociation; direct analysis in real time mass spectrometry; natural supplements; urine testing