title: Overexpression of microRNA-183 in human colorectal cancer and its clinical significance.
abstract: OBJECTIVE
  MicroRNA-183 (miR-183) has been shown to play a potential oncogenic role in colon cancer. The aim of this study was to evaluate the expression of miR-183 in colorectal cancer (CRC) and its potential relevance to clinicopathological characteristics and patient survival.
  
  
  METHODS
  Surgical specimens of cancer tissue and adjacent normal mucosa were obtained from 94 patients with CRC. The relative expression levels of miR-183 in the cancer and the normal adjacent mucosa were measured by quantitative real-time PCR. We analyzed their correlation with clinicopathological parameters and prognostic value.
  
  
  RESULTS
  The relative expression levels of miR-183 were significantly higher in CRC tissues than in the normal adjacent mucosa (P<0.001), and a high expression of miR-183 correlated with advanced clinical stage (P=0.030), lymph node metastasis (P=0.012), and distant metastasis (P=0.049). Kaplan-Meier analysis indicated that patients with high miR-183 expression had a poor overall survival (P=0.002). Moreover, multivariate analysis showed that increased expression of miR-183 was an independent predictor of overall survival.
  
  
  CONCLUSIONS
  The increased expression of miR-183 is closely related to advanced clinical stage, lymph node and distant metastases, and poor prognosis of CRC, indicating that miR-183 may serve as a predictive biomarker for the prognosis or the aggressiveness of CRC.
authors.affiliation: The First Department of General Surgery, Institute of Hepatobiliary, Pancreas and Intestinal Disease, The Affiliated Hospital of North Sichuan Medical College, Sichuan, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhou
authors.forename: Guang-Jun
authors.initials: GJ
authors.lastname: Zhang
authors.forename: He
authors.initials: H
authors.lastname: Zhou
authors.forename: Hua-Xu
authors.initials: HX
authors.lastname: Xiao
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000401:mortality / Q000556:secondary / Q000601:surgery; D002288:Adenocarcinoma, Mucinous / Q000235:genetics* / Q000401:mortality / Q000556:secondary / Q000601:surgery; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D016009:Chi-Square Distribution; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000032:analysis*; D008875:Middle Aged; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D016896:Treatment Outcome; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C533449:MIRN183 microRNA, human; D035683:MicroRNAs