title: Overproduction of geraniol by enhanced precursor supply in Saccharomyces cerevisiae.
abstract: Monoterpene geraniol, a compound obtained from aromatic plants, has wide applications. In this study, geraniol was synthesized in Saccharomyces cerevisiae through the introduction of geraniol synthase. To increase geraniol production, the mevalonate pathway in S. cerevisiae was genetically manipulated to enhance the supply of geranyl diphosphate, a substrate used for the biosynthesis of geraniol. Identification and optimization of the key regulatory points in the mevalonate pathway in S. cerevisiae increased geraniol production to 36.04 mg L(-1). The results obtained revealed that the IDI1-encoded isopentenyl diphosphate isomerase is a rate-limiting enzyme in the biosynthesis of geraniol in S. cerevisiae, and overexpression of MAF1, a negative regulator in tRNA biosynthesis, is another effective method to increase geraniol production in S. cerevisiae.
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education and School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; School of Light industry and Food Engineering, Guangxi University, 100 Daxue East Road, Nanning, Guangxi 530004, China.
authors.forename: Jidong
authors.initials: J
authors.lastname: Liu
authors.forename: Weiping
authors.initials: W
authors.lastname: Zhang
authors.forename: Guocheng
authors.initials: G
authors.lastname: Du
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Jingwen
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000080462:Acyclic Monoterpenes; D011756:Diphosphates / Q000378:metabolism; D004224:Diterpenes / Q000378:metabolism; D004926:Escherichia coli; D007535:Isomerases / Q000235:genetics / Q000378:metabolism; D060847:Metabolic Engineering; D008798:Mevalonic Acid / Q000737:chemistry / Q000378:metabolism*; D039821:Monoterpenes / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013729:Terpenes / Q000737:chemistry*
chemical_list: D000080462:Acyclic Monoterpenes; D011756:Diphosphates; D004224:Diterpenes; D039821:Monoterpenes; D013729:Terpenes; C511282:geranyl diphosphate; D010744:Phosphoric Monoester Hydrolases; D007535:Isomerases; C007836:geraniol; D008798:Mevalonic Acid
keywords: Geraniol; Metabolic engineering; Mevalonate pathway; Monoterpene; Saccharomyces cerevisiae