title: The need for transparency and good practices in the qPCR literature.
abstract: Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.
authors.affiliation: Postgraduate Medical Institute, Anglia Ruskin University, Chelmsford, UK.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Bustin
authors.forename: Vladimir
authors.initials: V
authors.lastname: Benes
authors.forename: Jeremy
authors.initials: J
authors.lastname: Garson
authors.forename: Jan
authors.initials: J
authors.lastname: Hellemans
authors.forename: Jim
authors.initials: J
authors.lastname: Huggett
authors.forename: Mikael
authors.initials: M
authors.lastname: Kubista
authors.forename: Reinhold
authors.initials: R
authors.lastname: Mueller
authors.forename: Tania
authors.initials: T
authors.lastname: Nolan
authors.forename: Michael W
authors.initials: MW
authors.lastname: Pfaffl
authors.forename: Gregory
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authors.lastname: Shipley
authors.forename: Carl T
authors.initials: CT
authors.lastname: Wittwer
authors.forename: Peter
authors.initials: P
authors.lastname: Schjerling
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Day
authors.forename: Mónica
authors.initials: M
authors.lastname: Abreu
authors.forename: Begoña
authors.initials: B
authors.lastname: Aguado
authors.forename: Jean-François
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authors.lastname: Beaulieu
authors.forename: Anneleen
authors.initials: A
authors.lastname: Beckers
authors.forename: Sara
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authors.lastname: Bogaert
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authors.lastname: Browne
authors.forename: Fernando
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authors.lastname: Carrasco-Ramiro
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authors.lastname: Ceelen
authors.forename: Kate
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authors.lastname: Ciborowski
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authors.lastname: Cornillie
authors.forename: Stephanie
authors.initials: S
authors.lastname: Coulon
authors.forename: Ann
authors.initials: A
authors.lastname: Cuypers
authors.forename: Sara
authors.initials: S
authors.lastname: De Brouwer
authors.forename: Leentje
authors.initials: L
authors.lastname: De Ceuninck
authors.forename: Jurgen
authors.initials: J
authors.lastname: De Craene
authors.forename: Hélène
authors.initials: H
authors.lastname: De Naeyer
authors.forename: Ward
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authors.lastname: De Spiegelaere
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authors.lastname: Deckers
authors.forename: Annelies
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authors.lastname: Dheedene
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authors.lastname: Durinck
authors.forename: Margarida
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authors.lastname: Ferreira-Teixeira
authors.forename: Annelies
authors.initials: A
authors.lastname: Fieuw
authors.forename: Jack M
authors.initials: JM
authors.lastname: Gallup
authors.forename: Sandra
authors.initials: S
authors.lastname: Gonzalo-Flores
authors.forename: Karen
authors.initials: K
authors.lastname: Goossens
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authors.lastname: Heindryckx
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authors.lastname: Herring
authors.forename: Hans
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authors.lastname: Hoenicka
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authors.lastname: Icardi
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authors.lastname: Jaggi
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authors.lastname: Javad
authors.forename: Michael
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authors.lastname: Karampelias
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authors.lastname: Kibenge
authors.forename: Molly
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authors.lastname: Kibenge
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authors.lastname: Kumps
authors.forename: Irina
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authors.lastname: Lambertz
authors.forename: Tim
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authors.lastname: Lammens
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authors.lastname: Markey
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authors.lastname: Messiaen
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authors.lastname: Mets
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authors.initials: S
authors.lastname: Morais
authors.forename: Alberto
authors.initials: A
authors.lastname: Mudarra-Rubio
authors.forename: Justine
authors.initials: J
authors.lastname: Nakiwala
authors.forename: Hilde
authors.initials: H
authors.lastname: Nelis
authors.forename: Pal A
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authors.lastname: Olsvik
authors.forename: Claudina
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authors.lastname: Pérez-Novo
authors.forename: Michelle
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authors.lastname: Plusquin
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authors.lastname: Remans
authors.forename: Ali
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authors.lastname: Rihani
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authors.lastname: Rodrigues-Santos
authors.forename: Pieter
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authors.lastname: Rondou
authors.forename: Rebecca
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authors.lastname: Sanders
authors.forename: Katharina
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authors.lastname: Schmidt-Bleek
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authors.forename: Karen
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authors.lastname: Smeets
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authors.lastname: Tabera
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authors.lastname: Van Acker
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authors.lastname: Van den Broeck
authors.forename: Joni
authors.initials: J
authors.lastname: Van der Meulen
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authors.lastname: Van Poucke
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authors.lastname: Van Roy
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authors.lastname: Vergult
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authors.lastname: Tshuikina-Wiklander
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authors.lastname: Willems
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authors.lastname: Zaccara
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authors.lastname: Zeka
authors.forename: Jo
authors.initials: J
authors.lastname: Vandesompele
mesh_terms: D003625:Data Collection; D007255:Information Services; D016133:Polymerase Chain Reaction / Q000379:methods*