title: Rutin activates the MAPK pathway and BDNF gene expression on beta-amyloid induced neurotoxicity in rats.
abstract: Flavonoids are present in foods such as fruits and vegetables. A relationship between the consumption of flavonoid-rich foods and prevention of human disease including neurodegenerative disorders has been demonstrated. We assessed the effect of rutin (3,3',4',5,7-pentahydroxyflavone-3-rhamnoglucoside) on the mitogen-activated protein kinase (MAPK) pathway, memory retrieval and oxidative stress in rats injected with β-amyloid (Aβ), which is implicated to have an important role in Alzheimer's disease (AD). Aβ was injected bilaterally in the deep frontal cortex of rat brain. Next, rutin and saline were injected (i.p.) for 3 weeks. In comparison to the control group, rutin significantly increased extracellular signal-regulated protein kinase 1 (ERK1), cAMP response element-binding protein (CREB) and brain-derived neurotrophic factor (BDNF) gene expression in the hippocampus of rats. Rutin (100 mg/kg) significantly increased memory retrieval compared to the control group. Malondialdehyde (MDA) level in the hippocampus of the rutin group was significantly lower than those in the control group. The content of sulfhydryl groups in the rutin group was higher than that in the control group. The findings show a possibility that rutin may have beneficial effects against neurotoxicity of Aβ on memory in rats.
authors.affiliation: Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran; Department of Genetics, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Sahar
authors.initials: S
authors.lastname: Moghbelinejad
authors.forename: Marjan
authors.initials: M
authors.lastname: Nassiri-Asl
authors.forename: Taghi Naserpour
authors.initials: TN
authors.lastname: Farivar
authors.forename: Esmail
authors.initials: E
authors.lastname: Abbasi
authors.forename: Mehdi
authors.initials: M
authors.lastname: Sheikhi
authors.forename: Mina
authors.initials: M
authors.lastname: Taghiloo
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Farsad
authors.forename: Amir
authors.initials: A
authors.lastname: Samimi
authors.forename: Farid
authors.initials: F
authors.lastname: Hajiali
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics*; D017362:Cyclic AMP Response Element-Binding Protein / Q000502:physiology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008297:Male; D020258:Neurotoxicity Syndromes / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D012431:Rutin / Q000494:pharmacology*
chemical_list: D016229:Amyloid beta-Peptides; D019208:Brain-Derived Neurotrophic Factor; D017362:Cyclic AMP Response Element-Binding Protein; D012431:Rutin; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: AD; Akt/PKB; Alzheimer's disease; Arc; Aβ; BDNF; CAT; CREB; ERK1/2; GPx; GSH; JNK; MAP kinase; MCAO; MDA; MEK1/2; Memory; Neurotoxicity; PBS; PI3K; PTZ; ROS; Rutin; SH; SOD; TrkB; activity-regulated cytoskeleton-associated protein; brain-derived neurotrophic factor; c-Jun N-terminal kinase; cAMP response element-binding protein; catalase; extracellular signal-regulated protein kinase; glutathione; glutathione peroxidase; i.p.; interaperitoneally; mTor; malondialdehyde; mammalian target of rapamycin; middle cerebral artery occlusion; mitogen activated kinase kinase; mitogen-activated protein kinase; pentylenetetrazole; phosphate buffered saline; phosphatidyl inositiol 3-kinase; protein kinase B; reactive oxygen species; sulfhydryl; superoxide dismutase; tropomyosin receptor kinase B; β-amyloid