title: TALEN-induced gene knock out in Drosophila.
abstract: We report here a case study of TALEN-induced gene knock out of the trachealess gene of Drosophila. Two pairs of TALEN constructs caused targeted mutation in the germ line of 39% and 17% of injected animals, respectively. In the extreme case 100% of the progeny of TALEN-injected fly was mutated, suggesting that highly efficient biallelic germ line mutagenesis was achieved. The mutagenic efficiency of the TALEN pairs paralleled their activity of single strand annealing (SSA) assay in cultured cells. All mutations were deletion of 1 to 20 base pairs. Merit and demerit of TALEN-based gene knockout approach compared to other genome editing technologies is discussed.
authors.affiliation: Laboratory for Morphogenetic Signaling, RIKEN Center for Developmental Biology, Kobe, Hyogo, 650-0047, Japan.
authors.forename: Takefumi
authors.initials: T
authors.lastname: Kondo
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Sakuma
authors.forename: Housei
authors.initials: H
authors.lastname: Wada
authors.forename: Ai
authors.initials: A
authors.lastname: Akimoto-Kato
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Shigeo
authors.initials: S
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics*; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D055786:Gene Knockout Techniques / Q000379:methods*
chemical_list: D004706:Endodeoxyribonucleases
keywords: Drosophila; TAL effector nuclease; genome editing