title: Utility of a fecal real-time PCR protocol for detection of Mycobacterium bovis infection in African buffalo (Syncerus caffer).
abstract: A real-time PCR protocol for detecting Mycobacterium bovis in feces was evaluated in bovine tuberculosis-infected African buffalo (Syncerus caffer). Fecal samples spiked with 1.42 × 10(3) cells of M. bovis culture/g and Bacille Calmette-Guérin standards with 1.58 × 10(1) genome copies/well were positive by real-time PCR but all field samples were negative.
authors.affiliation: 1  Wildlife Health Center & One Health Institute, School of Veterinary Medicine, 1089 Veterinary Medicine Drive, University of California, Davis, California 95616, USA.
authors.forename: Annette
authors.initials: A
authors.lastname: Roug
authors.forename: Claire
authors.initials: C
authors.lastname: Geoghegan
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Wellington
authors.forename: Woutrina A
authors.initials: WA
authors.lastname: Miller
authors.forename: Emma
authors.initials: E
authors.lastname: Travis
authors.forename: David
authors.initials: D
authors.lastname: Porter
authors.forename: David
authors.initials: D
authors.lastname: Cooper
authors.forename: Deana L
authors.initials: DL
authors.lastname: Clifford
authors.forename: Jonna A K
authors.initials: JA
authors.lastname: Mazet
authors.forename: Sven
authors.initials: S
authors.lastname: Parsons
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D009163:Mycobacterium bovis / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D014376:Tuberculosis / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*