title: Growth and differentiation of HaCaT keratinocytes.
abstract: HaCaT cells are a spontaneously immortalized, human keratinocyte line that has been widely used for studies of skin biology and differentiation. Under typical culture conditions HaCaT cells have a partially to fully differentiated phenotype due to the high calcium content of both standard media and fetal bovine serum. This chapter describes low-calcium culture conditions for reverting HaCaT cells to the fully basal state followed by subsequent controlled differentiation using calcium induction.
authors.affiliation: Department of Microbial Pathogenesis and Immunology, Texas A&M Health Science Center, College of Medicine, 8447 State Hwy 47, Medical Research & Education Building, Room # 1008, Bryan, TX, 77807, USA, Wilson@medicine.tamhsc.edu.
authors.forename: Van G
authors.initials: VG
authors.lastname: Wilson
mesh_terms: D002118:Calcium / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects; D010641:Phenotype
chemical_list: D002118:Calcium