title: [Effect of chronic intermittent hypoxia on the expression of fractalkine in rat liver].
abstract: OBJECTIVE
  To investigate the effect of chronic intermittent hypoxia (CIH) on liver injury and the expression of fractalkine in rats and explore its possible mechanism.
  
  
  METHODS
  A CIH murine model was established to mimic the pathophysiology of obstructive sleep apnea-hypopnea syndrome (OSAHS) in humans. Thirty healthy male Spraque-Dawley rats were randomly assigned to 3 groups: a 5% CIH group, a 5% CIH+RH (removal of hypoxia) group and a control group ( 10 rats in each group). The 5% CIH and 5% CIH+RH groups were exposed to CIH for 3 weeks, 8 h/d, and the frequency of hypoxia was 20 times/h. The 5% CIH+RH group was then exposed to normal gaseous environment for another 3 weeks. After the experiment, liver sections were stained with hematoxylin-eosin (HE) and the liver pathology was observed. The expression of fractalkine in the liver tissues was detected by immunohistochemical method.
  
  
  RESULTS
  1) Compared with the control group, the hepatic steatosis and inflammatory activities in the 5% CIH and 5% CIH+RH groups were more severe (all P<0.01 ); compared with the 5% CIH group, the hepatic steatosis and inflammatory activity in the 5% CIH+RH group were dramatically reduced (P<0.01 ). 2) Compared with the control group, the fractalkine expression in the 5% CIH and 5% CIH+RH groups was increased (both P<0.01). The fractalkine expression in the 5% CIH+RH group was dramatically downregulated compared with that in the 5% CIH group (P<0.01).
  
  
  CONCLUSIONS
  CIH can induce liver injury and high fractalkine expression in rat liver tissues.
authors.affiliation: Department of Emergency, Third Xiangya Hospital, Central South University, Changsha 410013,China.
authors.forename: Yayong
authors.initials: Y
authors.lastname: Li
authors.forename: Yina
authors.initials: Y
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.forename: Yingquan
authors.initials: Y
authors.lastname: Luo
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
mesh_terms: D000818:Animals; D054428:Chemokine CX3CL1 / Q000378:metabolism*; D004195:Disease Models, Animal; D005234:Fatty Liver; D000860:Hypoxia / Q000503:physiopathology*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D020181:Sleep Apnea, Obstructive / Q000503:physiopathology*
chemical_list: D054428:Chemokine CX3CL1; C515540:Cx3cl1 protein, rat