title: Neutron-encoded protein quantification by peptide carbamylation.
abstract: We describe a chemical tag for duplex proteome quantification using neutron encoding (NeuCode). The method utilizes the straightforward, efficient, and inexpensive carbamylation reaction. We demonstrate the utility of NeuCode carbamylation by accurately measuring quantitative ratios from tagged yeast lysates mixed in known ratios and by applying this method to quantify differential protein expression in mice fed a either control or high-fat diet.
authors.affiliation: Department of Chemistry, University of Wisconsin, Madison, WI, 53706, USA.
authors.forename: Arne
authors.initials: A
authors.lastname: Ulbrich
authors.forename: Anna E
authors.initials: AE
authors.lastname: Merrill
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Hebert
authors.forename: Michael S
authors.initials: MS
authors.lastname: Westphall
authors.forename: Mark P
authors.initials: MP
authors.lastname: Keller
authors.forename: Alan D
authors.initials: AD
authors.lastname: Attie
authors.forename: Joshua J
authors.initials: JJ
authors.lastname: Coon
mesh_terms: D000818:Animals; D059305:Diet, High-Fat; D007553:Isotope Labeling / Q000379:methods*; D013058:Mass Spectrometry; D051379:Mice; D009502:Neutrons; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: D011506:Proteins; D020543:Proteome