title: Anti-ghrelin immunoglobulins modulate ghrelin stability and its orexigenic effect in obese mice and humans.
abstract: Obese individuals often have increased appetite despite normal plasma levels of the main orexigenic hormone ghrelin. Here we show that ghrelin degradation in the plasma is inhibited by ghrelin-reactive IgG immunoglobulins, which display increased binding affinity to ghrelin in obese patients and mice. Co-administration of ghrelin together with IgG from obese individuals, but not with IgG from anorectic or control patients, increases food intake in rats. Similarly, chronic injections of ghrelin together with IgG from ob/ob mice increase food intake, meal frequency and total lean body mass of mice. These data reveal that in both obese humans and mice, IgG with increased affinity for ghrelin enhances ghrelin's orexigenic effect, which may contribute to increased appetite and overeating.
authors.affiliation: 1] Inserm UMR1073, Nutrition, Gut and Brain Laboratory, Rouen 76183, France [2] Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76183, France [3] Department of Psychosomatic Internal Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, Japan [4].
authors.forename: Kuniko
authors.initials: K
authors.lastname: Takagi
authors.forename: Romain
authors.initials: R
authors.lastname: Legrand
authors.forename: Akihiro
authors.initials: A
authors.lastname: Asakawa
authors.forename: Haruka
authors.initials: H
authors.lastname: Amitani
authors.forename: Marie
authors.initials: M
authors.lastname: François
authors.forename: Naouel
authors.initials: N
authors.lastname: Tennoune
authors.forename: Moïse
authors.initials: M
authors.lastname: Coëffier
authors.forename: Sophie
authors.initials: S
authors.lastname: Claeyssens
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: do Rego
authors.forename: Pierre
authors.initials: P
authors.lastname: Déchelotte
authors.forename: Akio
authors.initials: A
authors.lastname: Inui
authors.forename: Sergueï O
authors.initials: SO
authors.lastname: Fetissov
mesh_terms: D000818:Animals; D001066:Appetite; D016022:Case-Control Studies; D004435:Eating / Q000187:drug effects; D005247:Feeding Behavior; D005260:Female; D054439:Ghrelin / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000276:immunology* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020349:Surface Plasmon Resonance
chemical_list: D054439:Ghrelin; D007074:Immunoglobulin G