title: Engineering bioactive peptide-based therapeutic molecules.
abstract: Peptides are increasingly emerging as human therapeutic drugs. By screening very large phage display libraries, novel bioactive peptides that bind to the target of interest with desired biological properties can be identified. Peptides that are obtained in this fashion become the basis for therapeutic molecule development. However, naked peptides are usually not sufficient to be therapeutic molecules by themselves. They need to be chemically modified or conjugated to other molecules to obtain desired physicochemical and in vivo properties. In this chapter, we describe a general methodology of identifying bioactive peptides by biopanning of peptide phage libraries. As an example of therapeutic peptide modifications, we also describe a method for fusing the peptides to the Fc portion of antibody molecule to increase in vivo stability and activity.
authors.affiliation: Bio Lab, Emerging Technology Center, SAIT, Samsung Electronics, Giheung-Gu, Yongin-Si, Gyeonggi-Do, Republic of Korea.
authors.forename: Jong Sang
authors.initials: JS
authors.lastname: Ryu
authors.forename: A Yeon
authors.initials: AY
authors.lastname: Cho
authors.forename: Sang Won
authors.initials: SW
authors.lastname: Seo
authors.forename: Hosung
authors.initials: H
authors.lastname: Min
mesh_terms: D019921:Biotinylation; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D057809:HEK293 Cells; D006801:Humans; D055767:Immobilized Proteins / Q000378:metabolism; D010455:Peptides / Q000627:therapeutic use*; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA
chemical_list: D055767:Immobilized Proteins; D010455:Peptides; D011993:Recombinant Fusion Proteins