title: MicroRNA-127 is downregulated by Tudor-SN protein and contributes to metastasis and proliferation in breast cancer cell line MDA-MB-231.
abstract: Tudor-SN is a multifunctional protein that is highly expressed in multiple cancers including breast cancer. Tudor-SN, as a component in RNA-induced splicing complex, was recently reported to regulate gene expression in a microRNA (miRNA)-dependent manner, such as let-7, miR-34a and miR-221. However, how Tudor-SN is associated with cancer development still remains largely elusive. In the present study, we explored the role of Tudor-SN in breast cancer. Stable knockdown of endogenous Tudor-SN, performed on the breast cancer cell line MDA-MB-231 by small hairpin RNA expression vectors, suppressed the in vitro migration and invasion ability of the metastatic breast cancer cell line. Interestingly, we found Tudor-SN as a miRNA regulator according to microarray analysis, and further identified that Tudor-SN negatively regulated the expression of miR-127, and consequently increased the expression of the proto-oncogene BCL6 which was a convincing target of miR-127. Moreover, overexpression of miR-127 reduced the in vitro migration and proliferation ability of breast cancer cell MDA-MB-231. Collectively, our results suggested a novel mechanism that Tudor-SN promoted metastasis and proliferation of breast cancer cells via downregulating the miR-127 expression.
authors.affiliation: Department of cell biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, 300070, China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Zhao
authors.forename: Zhongchao
authors.initials: Z
authors.lastname: Duan
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Baoya
authors.initials: B
authors.lastname: Wang
authors.forename: Xinting
authors.initials: X
authors.lastname: Wang
authors.forename: Jinyan
authors.initials: J
authors.lastname: He
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yao
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002465:Cell Movement; D049109:Cell Proliferation; D004720:Endonucleases; D005260:Female; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D014945:Wound Healing
chemical_list: C000715470:MAS1 protein, human; C547276:MIRN127 microRNA, human; D035683:MicroRNAs; D009687:Nuclear Proteins; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D004720:Endonucleases; C095221:SND1 protein, human
keywords: Tudor-SN; breast cancer; metastasis; miR-127; proliferation