title: Silencing the glycerol-3-phosphate dehydrogenase gene in Saccharomyces cerevisiae results in more ethanol being produced and less glycerol.
abstract: Transcription of the gene coding for glycerol-3-phosphate dehydrogenase (GPD1) was repressed in an industrial strain of Saccharomyces cerevisiae using a silencing vector. A fusion fragment containing GPD1 and Kan MX genes was generated by overlap extension PCR, then, the vector, pYES2.0 GPD1/Kan MX, was constructed by inserting the fusion fragment into the S. cerevisiae plasmid, pYES2.0. pYES2.0 GPD1/Kan MX, was linearized by KpnI, transformed into S. cerevisiae using the PEG/LiAc/ssDNA method, and integrated into the S. cerevisiae chromosome. GPD1 silencing gave 20 % less glycerol-3-phosphate dehydrogenase activity, 19 % lower glycerol production, and 9.7 % higher ethanol production compared with the original strain. These findings further the development of industrial S. cerevisiae strains with improved ethanol production and reduced glycerol content for the efficient production of bio-ethanol.
authors.affiliation: College of Life Sciences, Fujian Normal University, Fuzhou, 350108, People's Republic of China, tingtxue@163.com.
authors.forename: Wenjin
authors.initials: W
authors.lastname: He
authors.forename: Shichao
authors.initials: S
authors.lastname: Ye
authors.forename: Ting
authors.initials: T
authors.lastname: Xue
authors.forename: Shengyan
authors.initials: S
authors.lastname: Xu
authors.forename: Weiyan
authors.initials: W
authors.lastname: Li
authors.forename: Jihua
authors.initials: J
authors.lastname: Lu
authors.forename: Luoyuan
authors.initials: L
authors.lastname: Cao
authors.forename: Bingying
authors.initials: B
authors.lastname: Ye
authors.forename: Youqiang
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000431:Ethanol / Q000378:metabolism*; D020868:Gene Silencing; D005990:Glycerol / Q000378:metabolism*; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+) / Q000037:antagonists & inhibitors* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; C568531:GPD1 protein, S cerevisiae; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+); D005990:Glycerol