title: Denitrification of industrial wastewater: Influence of glycerol addition on metabolic activity and community shifts in a microbial consortium.
abstract: The wastewater originating from explosives manufacturing plants are characterized by a high concentration of nitrates (3200mgNL(-1)), sulfates (1470mgL(-1)) and low pH (1.5) as well as the presence of organic compounds, such as nitroglycerin (1.9mgL(-1)) and nitroglycol (4.8mgL(-1)). The application of glycerol (C/N=3) at such a high concentration enabled complete removal of nitrates and did not cause the anaerobic glycerol metabolic pathway of the DNC4 consortium to activate, as confirmed by the low concentrations of 1,3-propanediol (0.16gL(-1)) and acetic acid (0.11gL(-1)) in the wastewater. Increasing the glycerol content (C/N=5) contributed to a notable increase in the concentration of both compounds: 1.12gL(-1) for acetic acid and 1.82 for 1,3-PD (1,3-propanediol). The nitrate reduction rate was at 44mgNg(-1) biomass d(-1). In order to assess the metabolic activity of the microorganisms, a method to determine the redox potential was employed. It was established, that the microorganisms can be divided into four groups, based on the determined denitrification efficiency and zero-order nitrate removal constants. The first group, involving Pseudomonas putida and Pseudomonas stutzeri, accounts for microorganisms capable of the most rapid denitrification, the second involves rapid denitrifying microbes (Citrobacter freundi and Pseudomonas alcaligenes), the third group are microorganisms exhibiting moderate denitrification ability: Achrobactrum xylosoxidans, Ochrobactrum intermedium and Stenotrophomonas maltophila, while the last group consists of slow denitrifying bacteria: Rodococcus rubber and Sphignobacterium multivorum.
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland. Electronic address: pcyplik@wp.pl.
authors.forename: Paweł
authors.initials: P
authors.lastname: Cyplik
authors.forename: Wojciech
authors.initials: W
authors.lastname: Juzwa
authors.forename: Roman
authors.initials: R
authors.lastname: Marecik
authors.forename: Jolanta
authors.initials: J
authors.lastname: Powierska-Czarny
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Piotrowska-Cyplik
authors.forename: Jakub
authors.initials: J
authors.lastname: Czarny
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Drożdżyńska
authors.forename: Lukasz
authors.initials: L
authors.lastname: Chrzanowski
mesh_terms: D058440:Denitrification; D005990:Glycerol / Q000378:metabolism*; D059013:Microbial Consortia / Q000502:physiology*; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism*; D012722:Sewage / Q000737:chemistry* / Q000382:microbiology; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000737:chemistry* / Q000382:microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
chemical_list: D005990:Glycerol; D009584:Nitrogen; D012722:Sewage; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Denitrification; Glycerol; Metabolic activity; Microbial consortium