title: The modulation of pancreatic lipase activity by alginates.
abstract: Alginates are comprised of mannuronic (M) and guluronic acid (G) and have been shown to inhibit enzyme activity. Pancreatic lipase is important in dietary triacylglycerol breakdown; reducing pancreatic lipase activity would reduce triacylglycerol breakdown resulting in lower amounts being absorbed by the body. Lipase activity in the presence of biopolymers was assessed by enzymatic assay using natural and synthetic substrates. Alginate inhibited pancreatic lipase by a maximum of 72.2% (±4.1) with synthetic substrate (DGGR) and 58.0% (±9.7) with natural substrate. High-G alginates from Laminaria hyperborea seaweed inhibited pancreatic lipase to a significantly higher degree than High-M alginates from Lessonia nigrescens, showing that inhibition was related to alginate structure. High-G alginates are effective inhibitors of pancreatic lipase and are used in the food industry at low levels. They could be included at higher levels in foods without altering organoleptic qualities, potentially reduce the uptake of dietary triacylglycerol aiding in weight management.
authors.affiliation: Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne, Tyne and Wear NE2 4HH, UK. Electronic address: matthew.wilcox@ncl.ac.uk.
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Wilcox
authors.forename: Iain A
authors.initials: IA
authors.lastname: Brownlee
authors.forename: J Craig
authors.initials: JC
authors.lastname: Richardson
authors.forename: Peter W
authors.initials: PW
authors.lastname: Dettmar
authors.forename: Jeffrey P
authors.initials: JP
authors.lastname: Pearson
mesh_terms: D000464:Alginates / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000032:analysis / Q000737:chemistry; D007700:Kinetics; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology*; D012624:Seaweed / Q000737:chemistry*
chemical_list: D000464:Alginates; D004791:Enzyme Inhibitors; D006603:Hexuronic Acids; C007896:guluronic acid; D020723:Glucuronic Acid; C008324:mannuronic acid; D008049:Lipase
keywords: Alginate; Inhibition; Lipase; Obesity; Weight management