title: Stimulation with monochromatic green light during incubation alters satellite cell mitotic activity and gene expression in relation to embryonic and posthatch muscle growth of broiler chickens.
abstract: Previous studies showed that monochromatic green light stimuli during embryogenesis accelerated posthatch body weight (BW) and pectoral muscle growth of broilers. In this experiment, we further investigated the morphological and molecular basis of this phenomenon. Fertile broiler eggs (Arbor Acres, n=880) were pre-weighed and randomly assigned to 1 of the 2 incubation treatment groups: (1) dark condition (control group), and (2) monochromatic green light group (560 nm). The monochromatic lighting systems sourced from light-emitting diode lamps and were equalized at the intensity of 15 lx at eggshell level. The dark condition was set as a commercial control from day 1 until hatching. After hatch, 120 male 1-day-old chicks from each group were housed under incandescent white light with an intensity of 30 lx at bird-head level. No effects of light stimuli during embryogenesis on hatching time, hatchability, hatching weight and bird mortality during the feeding trial period were observed in the present study. Compared with the dark condition, the BW, pectoral muscle weight and myofiber cross-sectional areas were significantly greater on 7-day-old chicks incubated under green light. Green light also increased the satellite cell mitotic activity of pectoral muscle on 1- and 3-day-old birds. In addition, green light upregulated MyoD, myogenin and myostatin mRNA expression in late embryos and/ or newly hatched chicks. These data suggest that stimulation with monochromatic green light during incubation promote muscle growth by enhancing proliferation and differentiation of satellite cells in late embryonic and newly hatched stages. Higher expression of myostatin may ultimately help prevent excessive proliferation and differentiation of satellite cells in birds incubated under green light.
authors.affiliation: 1 Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.affiliation: 1 Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: H J
authors.initials: HJ
authors.lastname: Zhang
authors.affiliation: 1 Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.affiliation: 1 Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: S G
authors.initials: SG
authors.lastname: Wu
authors.affiliation: 1 Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: X
authors.initials: X
authors.lastname: Qiao
authors.affiliation: 1 Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Yue
authors.affiliation: 2 College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: J H
authors.initials: JH
authors.lastname: Yao
authors.affiliation: 1 Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: G H
authors.initials: GH
authors.lastname: Qi
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000528:radiation effects; D002642:Chick Embryo / Q000196:embryology / Q000528:radiation effects*; D002645:Chickens / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000528:radiation effects*; D007150:Immunohistochemistry / Q000662:veterinary; D007185:Incubators / Q000662:veterinary; D008027:Light; D008297:Male; D008938:Mitosis / Q000528:radiation effects*; D018482:Muscle, Skeletal / Q000254:growth & development* / Q000528:radiation effects; D017570:MyoD Protein / Q000378:metabolism; D018008:Myogenin / Q000378:metabolism; D055435:Myostatin / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000528:radiation effects
chemical_list: D017570:MyoD Protein; D018008:Myogenin; D055435:Myostatin