title: Halo-alkalitolerant and thermostable cellulases with improved tolerance to ionic liquids and organic solvents from Paenibacillus tarimensis isolated from the Chott El Fejej, Sahara desert, Tunisia.
abstract: The wide number of industrial processes applying cellulases highlights the importance of discovering robust enzymes able to work under harsh conditions. In this study, carboxymethyl cellulase (CMCase) activity of Paenibacillus tarimensis was characterized. A high activity was observed in pH range 3.0-10.5 and 9 mM-5 M NaCl. In high salt buffer at 80°C, >80% and >76% of relative activity was retained at 20% of the ionic liquids (ILs) [EMIM]Ac and [BMIM]Cl; while >40% was detected with 40% [BMIM]Cl. Five CMCases were detected by renaturing SDS-PAGE. Their activity was retained in presence of 1.7 up to 5 M NaCl (for CMC1) or 4.6 M KCl; 5% organic solvents or 10 mM bivalent ions, EDTA and heavy metals; under neutral and halo-alkaline conditions. These cellulases stabile and highly functional under harsh conditions are promising candidates for application in detergents, textiles, paper/pulp industry; and simultaneous ILs treatment-saccharification of lignocellulose.
authors.affiliation: Dipartimento di Ingegneria Civile, Chimica, Ambientale e dei Materiali (DICAM), Università di Bologna, I-40131 Bologna, Italy. Electronic address: noura.raddadi@unibo.it.
authors.forename: Noura
authors.initials: N
authors.lastname: Raddadi
authors.forename: Ameur
authors.initials: A
authors.lastname: Cherif
authors.forename: Daniele
authors.initials: D
authors.lastname: Daffonchio
authors.forename: Fabio
authors.initials: F
authors.lastname: Fava
mesh_terms: D000468:Alkalies / Q000494:pharmacology*; D002480:Cellulase / Q000096:biosynthesis / Q000378:metabolism*; D003460:Crystallization; D003889:Desert Climate; D003902:Detergents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D052578:Ionic Liquids / Q000494:pharmacology*; D019216:Metals, Heavy / Q000494:pharmacology; D008969:Molecular Sequence Data; D009930:Organic Chemicals / Q000494:pharmacology; D056507:Paenibacillus / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D020673:Protein Renaturation / Q000187:drug effects; D055049:Salt Tolerance / Q000187:drug effects*; D012965:Sodium Chloride / Q000494:pharmacology; D012997:Solvents / Q000494:pharmacology*; D013379:Substrate Specificity / Q000187:drug effects; D013696:Temperature; D014416:Tunisia
chemical_list: D000468:Alkalies; D003902:Detergents; D052578:Ionic Liquids; D019216:Metals, Heavy; D009930:Organic Chemicals; D012997:Solvents; D012965:Sodium Chloride; D002480:Cellulase; C019826:carboxymethylcellulase
keywords: Chott; Ionic liquids; Paenibacillus tarimensis; Poly-extremotolerant cellulases