title: Gymnaster koraiensis and its major components, 3,5-di-O-caffeoylquinic acid and gymnasterkoreayne B, reduce oxidative damage induced by tert-butyl hydroperoxide or acetaminophen in HepG2 cells.
abstract: We investigated the protective effects of Gymnaster koraiensis against oxidative stress-induced hepatic cell damage. We used two different cytotoxicity models, i.e., the administration of tert-butyl hydroperoxide (t-BHP) and acetaminophen, in HepG2 cells to evaluate the protective effects of G. koraiensis. The ethyl acetate (EA) fraction of G. koraiensis and its major compound, 3,5-di-O-caffeoylquinic acid (DCQA), exerted protective effects in the t-BHP-induced liver cytotoxicity model. The EA fraction and DCQA ameliorated t-BHP-induced reductions in GSH levels and exhibited free radical scavenging activity. The EA fraction and DCQA also significantly reduced t-BHP-induced DNA damage in HepG2 cells. Furthermore, the hexane fraction of G. koraiensis and its major compound, gymnasterkoreayne B (GKB), exerted strong hepatoprotection in the acetaminophen-induced cytotoxicity model. CYP 3A4 enzyme activity was strongly inhibited by the extract, hexane fraction, and GKB. The hexane fraction and GKB ameliorated acetaminophen-induced reductions in GSH levels and protected against cell death.
authors.affiliation: Functional Food Center, Korea Institute of Science and Technology, Gangneung 210-340, Korea cwnho@kist.re.kr.
authors.forename: Eun Hye
authors.initials: EH
authors.lastname: Jho
authors.forename: Kyungsu
authors.initials: K
authors.lastname: Kang
authors.forename: Sarangerel
authors.initials: S
authors.lastname: Oidovsambuu
authors.forename: Eun Ha
authors.initials: EH
authors.lastname: Lee
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Jung
authors.forename: Il-Shik
authors.initials: IS
authors.lastname: Shin
authors.forename: Chu Won
authors.initials: CW
authors.lastname: Nho
mesh_terms: D000082:Acetaminophen / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D053279:Polyynes / Q000494:pharmacology*; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D020122:tert-Butylhydroperoxide / Q000494:pharmacology*
chemical_list: C472707:caffeoylquinic acid; C579347:gymnasterkoreayne B; D011801:Quinic Acid; D053279:Polyynes; D000082:Acetaminophen; D020122:tert-Butylhydroperoxide