title: Purification and identification of anti-oxidant soybean peptides by consecutive chromatography and electrospray ionization-mass spectrometry.
abstract: Ultrafiltration, ion-exchange chromatography, and gel filtration chromatography were used to purify anti-oxidant peptides from hydrolysates of soybean protein isolates. The <1-kD peptides were found to exhibit much higher anti-oxidant activity as compared to larger ones. Also, the alkaline peptide fractions were shown to have stronger anti-oxidant capacity than acidic and neutral peptides. Interestingly, an anti-oxidant tripeptide, Ser-Phe-Val (352.4 Da), was identified by reversed-phase high-performance liquid chromatography (RP-HPLC) connected online to electrospray ionization mass spectrometry. The tripeptide was further prepared by fluorenylmethoxycarbonyl (Fmoc) solid-phase synthesis and was found to have a dose-dependent protective effect against hydrogen peroxide (H2O2)-induced injuries in rat adrenal pheochromocytoma (PC12) cells.
authors.affiliation: 1 College of Light Industry and Food Sciences, South China University of Technology , Guangzhou, China .
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.forename: Mouming
authors.initials: M
authors.lastname: Zhao
authors.forename: Yalan
authors.initials: Y
authors.lastname: Cao
authors.forename: Yunjun
authors.initials: Y
authors.lastname: Lu
authors.forename: Ming
authors.initials: M
authors.lastname: Liang
authors.forename: Zebo
authors.initials: Z
authors.lastname: Huang
authors.forename: Jiaoyan
authors.initials: J
authors.lastname: Ren
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008969:Molecular Sequence Data; D016716:PC12 Cells; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D051381:Rats; D013025:Glycine max / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000975:Antioxidants; D010455:Peptides