title: Single-channel electrophysiology of cell-free expressed ion channels by direct incorporation in lipid bilayers.
abstract: Single-channel electrophysiology with lipid bilayer systems requires ion channel expression, purification from cell culture, and reconstitution in proteoliposomes for delivery to a planar bilayer. Here we demonstrate that single-channel current measurements of the potassium channels KcsA and hERGS5-S6 can be obtained by direct insertion in interdroplet lipid bilayers from microliters of a cell-free expression medium.
authors.affiliation: Electronics and Computer Science, University of Southampton, Southampton, SO17 1BJ, UK. mdp@ecs.soton.ac.uk.
authors.forename: Mark S
authors.initials: MS
authors.lastname: Friddin
authors.forename: Natalie P
authors.initials: NP
authors.lastname: Smithers
authors.forename: Maïwenn
authors.initials: M
authors.lastname: Beaugrand
authors.forename: Isabelle
authors.initials: I
authors.lastname: Marcotte
authors.forename: Philip T F
authors.initials: PT
authors.lastname: Williamson
authors.forename: Hywel
authors.initials: H
authors.lastname: Morgan
authors.forename: Maurits R R
authors.initials: MR
authors.lastname: de Planque
mesh_terms: D002474:Cell-Free System; D007473:Ion Channels / Q000502:physiology*; D008051:Lipid Bilayers; D011510:Proteolipids
chemical_list: D007473:Ion Channels; D008051:Lipid Bilayers; D011510:Proteolipids; C020485:proteoliposomes