title: Interleukin-1β secretion in hippocampal sclerosis patients with mesial temporal lobe epilepsy.
abstract: Mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HS) is a common medically intractable epilepsy syndrome. Although pathogenesis of HS still remains highly controversial, genetics may play a role as a predisposing factor. Previous evidence in a Japanese population revealed that the homozygotes for allele T at position -511 of the interleukin (IL)-1β gene promoter region (IL-1β-511 T/T) confers susceptibility to the development of HS. However, whether this polymorphism has an effect on IL-1β levels in MTLEHS patients was not demonstrated. This study aimed to analyze the distribution of this particular polymorphism in a group of Turkish HS patients and correlate the polymorphism with IL-1β secretion from the lymphocytes, thus revealing a functional role for IL-1β in the etiopathogenesis of HS. A single base pair polymorphism at position -511 in the promoter region of the IL-1β gene was analyzed. The spontaneous and 1 ng/mL lipopolysaccharidestimulated production of IL-1β by peripheral blood mononuclear cells after 4 and 24 h of incubation were measured by ELISA method. The heterozygous type (-511 C/T) was the most common genotype. There was no difference in frequency of allele -511 T between patients and controls. Analysis of IL-1β levels, genotype and allele distributions showed no significant difference among the groups (P>0.05). Nevertheless, it was seen that patients who carry a T allele at position -511 of the IL-1β gene had increased IL-1β levels. T-allele carriage may be important. Only IL-1β secretion from the lymphocytes has been assessed in this study. Considering the importance of IL-1β in the etiopathogenesis of HS, further studies are needed to evaluate locally produced IL-1β levels.
authors.affiliation: Department of Pediatric Neurology, Izmir Katip Celebi University , Izmir.
authors.forename: Nihal Olgac
authors.initials: NO
authors.lastname: Dundar
authors.forename: Berrin
authors.initials: B
authors.lastname: Aktekin
authors.forename: Nilufer Cicek
authors.initials: NC
authors.lastname: Ekinci
authors.forename: Duygu
authors.initials: D
authors.lastname: Sahinturk
authors.forename: Ugur
authors.initials: U
authors.lastname: Yavuzer
authors.forename: Olcay
authors.initials: O
authors.lastname: Yegin
authors.forename: Senay
authors.initials: S
authors.lastname: Haspolat
keywords: hippocampal sclerosis; interleukin-1β; polymorphism; temporal lobe epilepsy