title: Adenosine capped QDs based fluorescent sensor for detection of dopamine with high selectivity and sensitivity.
abstract: Facile detection of dopamine (DA) in biological samples for diagnostics remains a challenge. This paper reported an effective fluorescent sensor based on adenosine capped CdSe/ZnS quantum dots (A-QDs) for highly sensitive detection of DA in human urine samples. In this assay, adenosine serves as a capping ligand or stabilizer for QDs to render high-quality QDs dispersed in water, and as a receptor for DA to attach DA onto the surface of A-QDs. DA molecules can bind to A-QDs via non-covalent bonding, leading to the fluorescence quenching of A-QDs due to electron transfer. The A-QDs based fluorescence probe showed a limit of detection (LOD) of ca. 29.3 nM for DA detection. This facile method exhibited high selectivity and anti-interference in the presence of amino acid, ascorbic acid (AA), uric acid (UA) and glucide with 100-fold higher concentration in PBS solution. Furthermore, it was also successfully used in the detection of DA in the human urine samples with quantitative recoveries (94.80-103.40%).
authors.affiliation: Institute of Applied Chemistry, Department of Chemistry, East China University of Science and Technology, Shanghai 200237, PR China. yli@ecust.edu.cn zhongxh@ecust.edu.cn.
authors.forename: Qin
authors.initials: Q
authors.lastname: Mu
authors.forename: Hu
authors.initials: H
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Shijian
authors.initials: S
authors.lastname: Ma
authors.forename: Xinhua
authors.initials: X
authors.lastname: Zhong
mesh_terms: D000241:Adenosine / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods* / Q000592:standards; D004298:Dopamine / Q000032:analysis* / Q000652:urine; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D045663:Quantum Dots / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D000241:Adenosine; D004298:Dopamine