title: Direct somatic embroygenesis from immature embryos of rosewood (Dalbergia latifolia Roxb.).
abstract: Direct regeneration of somatic embryos was obtained from immature zygotic embryos of Dalbergia latifolia. Immature embryos dissected from green pods 90 d after flowering gave the highest frequency of somatic embryo formation. Preculture on high 2,4-D medium for 4 weeks induced direct somatic embryogenesis, which was expressed during the second culture phase in the presence of low 2,4-D along with a high sucrose concentration. Embryos were separated and transferred to the maturation medium containing MS + 0.5-1.0 mg/L BAP, where embryos developed into plantlets. Somatic embryos failed to convert into complete plants without BAP treatment. This method of direct regeneration of somatic embryos without a callus phase has direct application for genetic manipulation studies.
authors.affiliation: , .
authors.forename: M
authors.initials: M
authors.lastname: Muralidhar Rao
authors.forename: G
authors.initials: G
authors.lastname: Lakshmi Sita