title: Molecular and morphological systematics of the sandfly Sergentomyia (Sintonius) clydei Sinton, 1928 and questions about its record in the Seychelles.
abstract: In the Phlebotomine sandflies, a few molecular studies related on the genus Sergentomyia have been published. The present study explored the genetic variability within Sergentomyia (Sintonius) clydei (Diptera, Psychodidae). The sampling included 15 populations originating from 12 countries. A morphological approach was coupled to the sequencing of two molecular markers (cytochrome b mtDNA and cacophony nuclear DNA). The most variable morphological characters resided in the cibarium of the females, especially (i) the pigment patch pattern and (ii) the number of cibarial teeth and denticles in the armature. However this morphological approach was unable to individualize any population within S. clydei. The NJ trees based on both molecular markers individualized the specimens from the Aldabra group of islands in the Seychelles. Surprisingly, cyt b variability was not compatible with the known data about the complete submersion of Aldabra occurring relatively recently some 125,000 years ago. The settlement of these islands by S. clydei from continental Africa, the Middle East or Asia, and the value of mtDNA markers are discussed.
authors.affiliation: Université de Reims Champagne-Ardenne, ANSES, EA4688 - USC "transmission vectorielle et épidémiosurveillance de maladies parasitaires (VECPAR)", SFR Cap Santé, 51, rue Cognacq-Jay, 51096 Reims Cedex, France.
authors.forename: J
authors.initials: J
authors.lastname: Depaquit
authors.affiliation: Université de Reims Champagne-Ardenne, ANSES, EA4688 - USC "transmission vectorielle et épidémiosurveillance de maladies parasitaires (VECPAR)", SFR Cap Santé, 51, rue Cognacq-Jay, 51096 Reims Cedex, France; MIVEGEC, UMR IRD 224-CNRS 5290-UM1-UM2, 911, avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France; Département de Biologie Animale, Faculté des Sciences, Université d'Antananarivo, Madagascar.
authors.forename: F J
authors.initials: FJ
authors.lastname: Randrianambinintsoa
authors.affiliation: Université de Reims Champagne-Ardenne, ANSES, EA4688 - USC "transmission vectorielle et épidémiosurveillance de maladies parasitaires (VECPAR)", SFR Cap Santé, 51, rue Cognacq-Jay, 51096 Reims Cedex, France; Laboratoire de Parasitologie-Mycologie (99UR/08-05), Département de Biologie Clinique, Faculté de Pharmacie de, Monastir 5000, Tunisia.
authors.forename: K
authors.initials: K
authors.lastname: Jaouadi
authors.affiliation: Université de Reims Champagne-Ardenne, ANSES, EA4688 - USC "transmission vectorielle et épidémiosurveillance de maladies parasitaires (VECPAR)", SFR Cap Santé, 51, rue Cognacq-Jay, 51096 Reims Cedex, France.
authors.forename: J
authors.initials: J
authors.lastname: Payard
authors.affiliation: Université de Reims Champagne-Ardenne, ANSES, EA4688 - USC "transmission vectorielle et épidémiosurveillance de maladies parasitaires (VECPAR)", SFR Cap Santé, 51, rue Cognacq-Jay, 51096 Reims Cedex, France; Laboratoire des Sciences Naturelles et Matériaux, Institut des Sciences et de la Technologie, Centre Universitaire de Mila BP26 RP, 43000 Mila, Algeria.
authors.forename: A
authors.initials: A
authors.lastname: Bounamous
authors.affiliation: Université de Reims Champagne-Ardenne, ANSES, EA4688 - USC "transmission vectorielle et épidémiosurveillance de maladies parasitaires (VECPAR)", SFR Cap Santé, 51, rue Cognacq-Jay, 51096 Reims Cedex, France.
authors.forename: D
authors.initials: D
authors.lastname: Augot
authors.affiliation: Bundeswehr Hospital Hamburg, Tropical Medicine Branch, Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Strasse 74, 20359 Hamburg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Krueger
authors.affiliation: MIVEGEC, UMR IRD 224-CNRS 5290-UM1-UM2, 911, avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France.
authors.forename: C
authors.initials: C
authors.lastname: Brengues
authors.affiliation: Centre National de séquençage, Génoscope, 91000 Evry, France.
authors.forename: A
authors.initials: A
authors.lastname: Couloux
authors.affiliation: MIVEGEC, UMR IRD 224-CNRS 5290-UM1-UM2, 911, avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France. Electronic address: vincent.robert@ird.fr.
authors.forename: V
authors.initials: V
authors.lastname: Robert
authors.affiliation: 63, avenue Pierre Sémard, 94210 La Varenne Saint Hilaire, France.
authors.forename: N
authors.initials: N
authors.lastname: Léger
mesh_terms: D000818:Animals; D005260:Female; D005819:Genetic Markers; D014644:Genetic Variation; D049750:Genome, Insect; D010802:Phylogeny; D058974:Phylogeography; D011576:Psychodidae / Q000145:classification* / Q000235:genetics / Q000502:physiology*; D017422:Sequence Analysis, DNA / Q000379:methods*; D012750:Seychelles
chemical_list: D005819:Genetic Markers
keywords: Aldabra atoll; Colonization; Island; Phlebotomine sandflies; Systematics; The Seychelles