title: Prevention of biofilm colonization by Gram-negative bacteria on minocycline-rifampin-impregnated catheters sequentially coated with chlorhexidine.
abstract: Resistant Gram-negative bacteria are increasing central-line-associated bloodstream infection threats. To better combat this, chlorhexidine (CHX) was added to minocycline-rifampin (M/R) catheters. The in vitro antimicrobial activity of CHX-M/R catheters against multidrug resistant, Gram-negative Acinetobacter baumannii, Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia was tested. M/R and CHX-silver sulfadiazine (CHX/SS) catheters were used as comparators. The novel CHX-M/R catheters were significantly more effective (P < 0.0001) than CHX/SS or M/R catheters in preventing biofilm colonization and showed better antimicrobial durability.
authors.affiliation: Department of Infectious Diseases, Infection Control and Employee Health, The University of Texas M. D. Anderson Cancer Center, Houston, Texas, USA.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Jamal
authors.forename: Joel S
authors.initials: JS
authors.lastname: Rosenblatt
authors.forename: Ray Y
authors.initials: RY
authors.lastname: Hachem
authors.forename: Jiang
authors.initials: J
authors.lastname: Ying
authors.forename: Egbert
authors.initials: E
authors.lastname: Pravinkumar
authors.forename: Joseph L
authors.initials: JL
authors.lastname: Nates
authors.forename: Anne-Marie P
authors.initials: AM
authors.lastname: Chaftari
authors.forename: Issam I
authors.initials: II
authors.lastname: Raad
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects / Q000254:growth & development; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D057785:Catheters / Q000382:microbiology*; D002710:Chlorhexidine / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003470:Culture Media; D004338:Drug Combinations; D016972:Enterobacter cloacae / Q000187:drug effects / Q000254:growth & development; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000254:growth & development; D008911:Minocycline / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D012293:Rifampin / Q000494:pharmacology*; D020615:Stenotrophomonas maltophilia / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D004338:Drug Combinations; D008911:Minocycline; D002710:Chlorhexidine; D012293:Rifampin