title: HSA nanocapsules functionalized with monoclonal antibodies for targeted drug delivery.
abstract: The chronic autoimmune disorder rheumatoid arthritis (RA) affects millions of adults and children every year. Chronically activated macrophages secreting enzymes and inflammatory cytokines play a key role in RA. Distinctive marker molecules on the macrophage surface could be used to design a targeted drug delivery device for the treatment of RA without affecting healthy cells and tissues. Here, different methods for covalent attachment of antibodies (mAb) recognizing MHC class II molecules found on macrophages onto human serum albumin (HSA) nanocapsules were compared. HSA nanocapsules were prepared with a hydrodynamic diameter of 500.7 ± 9.4 nm and a narrow size distribution as indicated by a polydispersity index (PDI) of 0.255 ± 0.024. This was achieved by using a sonochemical process avoiding toxic cross linking agents and emulsifiers. Covalent binding of mAb on the surface of HSA nanocapsules was realized using polyethyleneglycol (PEG)3000 as spacer molecule. The presence of mAb was confirmed by confocal laser scanning microscopy (CLSM) and enzyme-linked immunosorbent assay (ELISA). Specific binding of mAb-HSA nanocapsules to MHC class II molecules on antigen-presenting cells was demonstrated by flow cytometry analysis.
authors.affiliation: University of Natural Resources and Life Sciences, Institute for Environmental Biotechnology, Konrad Lorenz Street 20, 3430 Tulln, Austria.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Rollett
authors.forename: Tamara
authors.initials: T
authors.lastname: Reiter
authors.forename: Anna
authors.initials: A
authors.lastname: Ohradanova-Repic
authors.forename: Christian
authors.initials: C
authors.lastname: Machacek
authors.forename: Artur
authors.initials: A
authors.lastname: Cavaco-Paulo
authors.forename: Hannes
authors.initials: H
authors.lastname: Stockinger
authors.forename: Georg M
authors.initials: GM
authors.lastname: Guebitz
mesh_terms: D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000737:chemistry; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells; D008264:Macrophages / Q000187:drug effects; D053769:Nanocapsules / Q000008:administration & dosage* / Q000737:chemistry; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D012709:Serum Albumin / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D053769:Nanocapsules; D012709:Serum Albumin; D011092:Polyethylene Glycols
keywords: Albumin nanocapsules; Antibody; Cross linking; Drug delivery; Targeted