title: A genetic map of potato (Solanum tuberosum) integrating molecular markers, including transposons, and classical markers.
abstract: A genetic map of potato (Solanum tuberosum L.) integrating molecular markers with morphological and isozyme markers was constructed using a backcross population of 67 diploid potato plants. A general method for map construction is described that differs from previous methods employed in potato and other outbreeding plants. First, separate maps for the female and male parents were constructed. The female map contained 132 markers, whereas the male map contained 138 markers. Second, on the basis of the markers in common the two integrated parental maps were combined into one with the computer programme JoinMap. This combined map consisted of 175 molecular markers, 10 morphological markers and 8 isozyme markers. Ninety-two of the molecular markers were derived from DNA sequences flanking either T-DNA inserts in potato or reintegrated maize transposable elements originating from these T-DNA constructs. Clusters of distorted segregation were found on chromosomes 1,2,8 and 11 for the male parent and chromosome 5 for both parents. The total length of the combined map is 1120 cM.
authors.affiliation: Department of Molecular Biology, DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, NL-6700, AA Wageningen, The Netherlands.
authors.forename: J M
authors.initials: JM
authors.lastname: Jacobs
authors.forename: H J
authors.initials: HJ
authors.lastname: Van Eck
authors.forename: P
authors.initials: P
authors.lastname: Arens
authors.forename: B
authors.initials: B
authors.lastname: Verkerk-Bakker
authors.forename: B
authors.initials: B
authors.lastname: Te Lintel Hekkert
authors.forename: H J
authors.initials: HJ
authors.lastname: Bastiaanssen
authors.forename: A
authors.initials: A
authors.lastname: El-Kharbotly
authors.forename: A
authors.initials: A
authors.lastname: Pereira
authors.forename: E
authors.initials: E
authors.lastname: Jacobsen
authors.forename: W J
authors.initials: WJ
authors.lastname: Stiekema