title: In vitro effects of vitamin supplements on platelet-activating factor and its metabolism in age-related macular degeneration.
abstract: OBJECTIVE
  The purpose of our study was to investigate for the first time a series of vitamin supplements used for age-related macular degeneration (AMD) as potential inhibitors of platelet-activating factor (PAF).
  
  
  METHODS
  Various vitamin supplements were tested in washed rabbit platelets (WRPs), in order to investigate the interaction between vitamin supplements (InShape, Nutrof, Ocuvite, Vitalux) and inhibition of PAF-induced platelet aggregation. Additionally, we examined their ability to affect PAF-metabolism, through their in vitro effect on PAF basic metabolic enzymes (PAF-CPT, lyso PAF-AT, and PAF-AH).
  
  
  RESULTS
  Nutrof exhibited the strongest anti-PAF activity, while Vitalux was the most potent anti-inflammatory factor.
  
  
  CONCLUSIONS
  This is the first study to bring in surface potent anti-inflammatory and anti-angiogenic activities of some vitamin supplements used against AMD, through their in vitro anti-PAF effects in WRPs and the rabbit plasma and leukocyte PAF metabolism, suggesting a promising role of vitamin supplements and especially resveratrol, concerning its potent anti-angiogenic activity in AMD.
authors.affiliation: Laboratory of Electrophysiology, 1st Department of Ophthalmology, University of Athens , Athens , Greece and.
authors.forename: Marilita M
authors.initials: MM
authors.lastname: Moschos
authors.forename: Irini P
authors.initials: IP
authors.lastname: Chatziralli
authors.forename: George
authors.initials: G
authors.lastname: Stamatakis
authors.forename: Vasiliki D
authors.initials: VD
authors.lastname: Papakonstantinou
authors.forename: Constantinos A
authors.initials: CA
authors.lastname: Demopoulos
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D019587:Dietary Supplements; D008268:Macular Degeneration; D010972:Platelet Activating Factor / Q000031:analogs & derivatives; D010974:Platelet Aggregation / Q000187:drug effects*; D011817:Rabbits; D014815:Vitamins / Q000494:pharmacology*
chemical_list: C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor; D014815:Vitamins
keywords: Age-related macular degeneration; InShape; Nutrof; Ocuvite; Vitalux; metabolism; platelet-activating factor; vitamin