title: Flagella as a novel alignment medium for the measurement of residual dipolar couplings in proteins.
abstract: The two flexible rod-like flagella (∼500 nm in diameter and 5-15 μm long) of Chlamydomonas reinhardtii, a unicellular green alga, can weakly align molecules in an external magnetic field, thereby enabling the measurement of various residual dipolar couplings in solution NMR spectroscopy.
authors.affiliation: Tata Institute of Fundamental Research, Mumbai, India. chary@tifr.res.in.
authors.forename: Himanshu
authors.initials: H
authors.lastname: Singh
authors.forename: Manish
authors.initials: M
authors.lastname: Shukla
authors.forename: Basuthkar J
authors.initials: BJ
authors.lastname: Rao
authors.forename: Kandala V R
authors.initials: KV
authors.lastname: Chary
mesh_terms: D016825:Chlamydomonas reinhardtii / Q000502:physiology*; D005407:Flagella / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D009587:Nitrogen Isotopes / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D025801:Ubiquitin / Q000737:chemistry / Q000378:metabolism
chemical_list: D009587:Nitrogen Isotopes; D010940:Plant Proteins; D025801:Ubiquitin