title: [Identification of contraction related proteins in corpus myometrium at labor].
abstract: OBJECTIVE
  To explore the molecular mechanism of human corpus myometrium contraction related proteins and parturition.
  
  
  METHODS
  The proteins of human corpus of myometrium tissues from full term non-in labor (38- 41 weeks amenorrhea) and full term in labor (38-41 weeks amenorrhea) gravidas were separated by 2-dimensional gel electrophoresis (2-GE), respectively. Then gels were stained by Coomassie brilliant blue G250, scanned by Image scanner and analyzed with PDQuest software. The differentially expressed protein spots of corpus myometrium between the 2 groups were identified by peptide mass finger print based on matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and database searching. Three identified differentially expressed proteins were confirmed by Western blot.
  
  
  RESULTS
  Well resolved and reproducible 2D-GE maps of human corpus myometrium from non-in labor and in-labor gravidas were acquired. Twenty more than 2-fold differentially expressed protein spots were identified by MALDI-TOF-MS. These proteins were involved in cell structure, calcium binding, chaperone, energy metabolism, signal transduction, and antioxidant.
  
  
  CONCLUSIONS
  Twenty contraction related proteins of human corpus myometrium have been identified, indicating that cell structure and calcium-binding are important reasons for the contraction of human corpus myometrium.
authors.affiliation: Medical School of Northwest University for Nationalities, Lanzhou 730030; Department of Obstetrics and Gynecology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.forename: Changju
authors.initials: C
authors.lastname: Zhou
mesh_terms: D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007743:Labor, Obstetric / Q000502:physiology*; D013058:Mass Spectrometry; D009119:Muscle Contraction; D009124:Muscle Proteins / Q000032:analysis*; D009215:Myometrium / Q000502:physiology*; D011247:Pregnancy; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D009124:Muscle Proteins