title: HIV-1 Rev function and RNA nuclear-cytoplasmic export.
abstract: The requirement of HIV-1 and HTLV-1 to export incompletely spliced mRNAs has necessitated the evolution of Rev and Rex, respectively, to overcome host cellular mechanism that block nuclear-cytoplasmic export of incompletely processed mRNAs. Evaluating the function of these viral factors can be done at multiple levels: examining the functional consequence of Rev/Rex on viral gene expression, monitoring the movement of these proteins between the nucleus and cytoplasm or the subcellular distribution of the viral mRNAs. Here, I describe procedures to evaluate each of these aspects of Rev/Rex function.
authors.affiliation: Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
authors.forename: Alan
authors.initials: A
authors.lastname: Cochrane
mesh_terms: D021581:Active Transport, Cell Nucleus; D002467:Cell Nucleus / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D057809:HEK293 Cells; D015497:HIV-1 / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D017403:In Situ Hybridization; D009068:Movement; D015347:RNA Probes / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D054321:rev Gene Products, Human Immunodeficiency Virus / Q000378:metabolism*
chemical_list: D015347:RNA Probes; D012367:RNA, Viral; D054321:rev Gene Products, Human Immunodeficiency Virus; C514860:rev protein, Human Immunodeficiency Virus-1