title: Mouse dental pulp stem cells support human umbilical cord blood-derived hematopoietic stem/progenitor cells in vitro.
abstract: It is well documented that specialized mesenchymal stem/stromal cells (MSCs) constitute the hematopoietic stem cell (HSC) niche in the bone marrow (BM), and these MSCs support/maintain the HSCs in an undifferentiated state. A number of studies have demonstrated that BM-derived MSCs (BM-MSCs) can support HSCs in vitro. However, it remains unclear whether nonhematopoietic tissue-derived MSC-like cells, such as dental pulp stem cells (DPSCs), have the ability to support HSCs. In this study, we prospectively isolated DPSCs from mouse mandibular incisors by fluorescence-activated cell sorting (FACS) using BM-MSC markers, such as PDGFRα and Sca-1. The PDGFRα and Sca-1 double-positive DPSCs and BM-MSCs showed similar morphologies and expression patterns of MSC markers. The ability of the DPSCs to support hematopoietic stem/progenitor cells (HSPCs) was then analyzed by an in vitro coculture system. Moreover, their HSC-supporting activity was evaluated by in vivo xenotransplantation assays using NOD/Shi-scid/IL-2Rγc(null) (NOG) mice. Interestingly, the DPSCs supported human cord blood (CB)-derived CD34-positive (CD34(+)), as well as CD34-negative (CD34(-)), HSCs. The supporting activities of DPSCs for human CB-derived CD34(+) and CD34(-) HSCs were comparable to those of BM-MSCs. The results of the present study demonstrated, for the first time, that prospectively isolated murine PDGFRα and Sca-1 double-positive DPSCs could support primitive human CD34(+) and CD34(-) HSCs in vitro.
authors.affiliation: Department of Stem Cell Biology and Regenerative Medicine, Graduate School of Medical Science, Kansai Medical University, Hirakata, Osaka, Japan.
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Nakatsuka
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Matsuoka
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Uemura
authors.forename: Keisuke
authors.initials: K
authors.lastname: Sumide
authors.forename: Ryuji
authors.initials: R
authors.lastname: Iwaki
authors.forename: Masaya
authors.initials: M
authors.lastname: Takahashi
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Fujioka
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Sonoda
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D002478:Cells, Cultured; D018920:Coculture Techniques; D036101:Cord Blood Stem Cell Transplantation; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005312:Fetal Blood; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D064593:Heterografts; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D016513:Mice, SCID
chemical_list: D000943:Antigens, Differentiation