title: From Agrobacterium to viral vectors: genome modification of plant cells by rare cutting restriction enzymes.
abstract: Researchers and biotechnologists require methods to accurately modify the genome of higher eukaryotic cells. Such modifications include, but are not limited to, site-specific mutagenesis, site-specific insertion of foreign DNA, and replacement and deletion of native sequences. Accurate genome modifications in plant species have been rather limited, with only a handful of plant species and genes being modified through the use of early genome-editing techniques. The development of rare-cutting restriction enzymes as a tool for the induction of site-specific genomic double-strand breaks and their introduction as a reliable tool for genome modification in animals, animal cells and human cell lines have paved the way for the adaptation of rare-cutting restriction enzymes to genome editing in plant cells. Indeed, the number of plant species and genes which have been successfully edited using zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and engineered homing endonucleases is on the rise. In our review, we discuss the basics of rare-cutting restriction enzyme-mediated genome-editing technology with an emphasis on its application in plant species.
authors.affiliation: Danziger Innovations Ltd., Mishmar Hashiva Village, Beit Dagan, Israel.
authors.forename: Ira
authors.initials: I
authors.lastname: Marton
authors.forename: Arik
authors.initials: A
authors.lastname: Honig
authors.forename: Ayelet
authors.initials: A
authors.lastname: Omid
authors.forename: Noam
authors.initials: N
authors.lastname: De Costa
authors.forename: Elena
authors.initials: E
authors.lastname: Marhevka
authors.forename: Barry
authors.initials: B
authors.lastname: Cohen
authors.forename: Amir
authors.initials: A
authors.lastname: Zuker
authors.forename: Alexander
authors.initials: A
authors.lastname: Vainstein
mesh_terms: D060054:Agrobacterium / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001709:Biotechnology / Q000379:methods; D018556:Crops, Agricultural / Q000235:genetics; D004260:DNA Repair; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D005786:Gene Expression Regulation; D017343:Genes, Plant; D005822:Genetic Vectors; D016678:Genome; D018745:Genome, Plant; D023281:Genomics; D006801:Humans; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics; D012313:RNA / Q000378:metabolism; D011995:Recombination, Genetic; D015533:Transcriptional Activation; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; C036483:T-DNA; D012313:RNA; D004262:DNA Restriction Enzymes