title: Silica coating of the pore walls of a microporous polycaprolactone membrane to be used in bone tissue engineering.
abstract: Polycaprolactone/silica microporous hybrid membranes were produced in two steps: A microporous polycaprolactone membrane with an interconnected porosity of 80% was obtained via a freeze extraction procedure, then silica was formed by a sol-gel reaction inside the micropores using tetraethyl orthosilicate, TEOS, as silica precursor. It is shown that silica forms a thin coating layer homogeneously distributed over the pore walls when sol-gel reaction is catalyzed by hydrochloric acid, while it forms submicron spherical particles when using basic catalyzer. Some physical properties and the viability and osteoblastic differentiation of bone marrow rat mesenchymal stem cells cultured on pure and hybrid membranes were studied.
authors.affiliation: Ankara University, Stem Cell Institute, TEBN Laboratory, Ankara, Turkey; Department of Chemistry, Ankara University, Faculty of Science, TEBN Laboratory, Ankara, Turkey.
authors.forename: B
authors.initials: B
authors.lastname: Demirdögen
authors.forename: C E Plazas
authors.initials: CE
authors.lastname: Bonilla
authors.forename: S
authors.initials: S
authors.lastname: Trujillo
authors.forename: J E
authors.initials: JE
authors.lastname: Perilla
authors.forename: A E
authors.initials: AE
authors.lastname: Elcin
authors.forename: Y M
authors.initials: YM
authors.lastname: Elcin
authors.forename: J L Gómez
authors.initials: JL
authors.lastname: Ribelles
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D008567:Membranes, Artificial; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D012822:Silicon Dioxide / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D011091:Polyesters; C016240:polycaprolactone; D012822:Silicon Dioxide
keywords: hybrid composites; mesenchymal stem cells; osteogenic differentiation; polycaprolactone-silica; silica coating