title: Distribution of Virulence Genes and Their Association of Serotypes in Pathogenic Escherichia coli Isolates From Diarrheal Patients in Korea.
abstract: OBJECTIVE
  To characterise the genetic and serological diversity of pathogenic Escherichia coli, we tested 111 E coli strains isolated from diarrhoeal patients in Korea between 2003 and 2006.
  
  
  METHODS
  The isolates were tested through polymerase chain reaction (PCR) and slide agglutination method for the detection of virulence genes and serotypes, respectively. To compare the expression of Shiga toxin (stx)-1 and stx2 genes, real-time quantitative reverse-transcriptase PCR and rapid exprssion assay, reversed-passive latex agglutination, were performed.
  
  
  RESULTS
  Forty-nine Shiga toxin-producing E coli (STEC) strains and 62 non-STEC strains, including 20 enteropathogenic E coli, 20 enterotoxigenic E coli, 20 enteroaggregative E coli, and 2 enteroinvasive E coli were randomly chosen from the strains isolated from diarrhoeal patients in Korea between 2003 and 2006. PCR analysis indicated that locus of enterocyte effacement pathogenicity island, that is, eaeA, espADB, and tir genes were present in STEC, enteropathogenic E coli, and enteroinvasive E coli. Quorum sensing-related gene luxS was detected in most of pathogenic E coli strains. Major serotypes of the STEC strains were O157 (26%) and O26 (20%), whereas the non-STEC strains possessed various serotypes. Especially, all the strains with serotype O157 carried stx2 and the tested virulence factors. Of the STEC strains, the data of real-time quantitative reverse-transcriptase PCR and reversed-passive latex agglutination tests showed that messenger RNA- and protein expression of stx2 gene were higher than those of stx1 gene.
  
  
  CONCLUSIONS
  Our results provide the epidemiological information regarding the trend of STEC and non-STEC infections in the general population and show the fundamental data in association of serotypes with virulence genes in diarrhoeagenic E coli strains from Korea.
authors.affiliation: Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korean National Institute of Health, Korea Centers for Disease Control & Prevention, Korea.
authors.forename: Seung-Hak
authors.initials: SH
authors.lastname: Cho
authors.forename: Kyung-Hwan
authors.initials: KH
authors.lastname: Oh
authors.forename: Seong-Han
authors.initials: SH
authors.lastname: Kim
authors.forename: Hee-Bok
authors.initials: HB
authors.lastname: Oh
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Park
keywords: diarrhoeal patients; pathogenic Escherichia coli; serotypes; virulence factors