title: Prevalence of the HOXB13 G84E prostate cancer risk allele in men treated with radical prostatectomy.
abstract: OBJECTIVE
  To determine the prevalence and clinical correlates of the G84E mutation in the homeobox transcription factor, or HOXB13, gene using DNA samples from 9559 men with prostate cancer undergoing radical prostatectomy.
  
  
  METHODS
  DNA samples from men treated with radical prostatectomy at the University of Michigan and John Hopkins University were genotyped for G84E and this was confirmed by Sanger sequencing. The frequency and distribution of this allele was determined according to specific patient characteristics (family history, age at diagnosis, pathological Gleason grade and stage).
  
  
  RESULTS
  Of 9559 patients, 128 (1.3%) were heterozygous carriers of G84E. Patients who possessed the variant were more likely to have a family history of prostate cancer than those who did not (46.0 vs 35.4%; P = 0.006). G84E carriers were also more likely to be diagnosed at a younger age than non-carriers (55.2 years vs 58.1 years; P < 0.001). No difference in the proportion of patients diagnosed with high grade or advanced stage tumours according to carrier status was observed.
  
  
  CONCLUSIONS
  In the present study, carriers of the rare G84E variant in HOXB13 were both younger at the time of diagnosis and more likely to have a family history of prostate cancer compared with homozygotes for the wild-type allele. No significant differences in allele frequency were detected according to selected clinical characteristics of prostate cancer. Further investigation is required to evaluate the role of HOXB13 in prostate carcinogenesis.
authors.affiliation: Department of Oncology, Wayne State University, Detroit, MI, USA; Barbara Ann Karmanos Cancer Institute, Population Studies and Disparities Research Program, Detroit, MI, USA.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Beebe-Dimmer
authors.forename: William B
authors.initials: WB
authors.lastname: Isaacs
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Zuhlke
authors.forename: Cecilia
authors.initials: C
authors.lastname: Yee
authors.forename: Patrick C
authors.initials: PC
authors.lastname: Walsh
authors.forename: Sarah D
authors.initials: SD
authors.lastname: Isaacs
authors.forename: Anna M
authors.initials: AM
authors.lastname: Johnson
authors.forename: Charles E
authors.initials: CE
authors.lastname: Ewing
authors.forename: Elizabeth B
authors.initials: EB
authors.lastname: Humphreys
authors.forename: Wasim H
authors.initials: WH
authors.lastname: Chowdhury
authors.forename: James E
authors.initials: JE
authors.lastname: Montie
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: Cooney
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000235:genetics*; D004252:DNA Mutational Analysis; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D015995:Prevalence; D011468:Prostatectomy / Q000379:methods*; D011471:Prostatic Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000601:surgery; D012189:Retrospective Studies; D012307:Risk Factors; D014481:United States / Q000453:epidemiology
chemical_list: D014408:Biomarkers, Tumor; C484414:HOXB13 protein, human; D018398:Homeodomain Proteins; D004247:DNA
keywords: HOXB13; family history; genetic epidemiology; prostate cancer