title: Evidence for a geranyl-diphosphate synthase located within the plastids of Vitis vinifera L. cultivated in vitro.
abstract: Intact plastids from cell suspensions of Vitis vinifera L. cv. Muscat de Frontignan, free of detectable contamination by other particles as judged by the distribution of organelle-specific marker enzymes and by electron microscopy, exhibit geranyl-diphosphate synthase activity (EC 2.5.1.1). This synthase activity remains stable after tryptic digestion of unlysed organelles and is enhanced by plastid disruption. We conclude that the enzyme is located within the organelle. The possibility of an isopentenyl diphosphate/dimethylallyl diphosphate translocating system which would play a major role in the regulation of monoterpene metabolism is discussed.
authors.affiliation: Laboratoire de Biotechnologie et Amélioration des Plantes, Ecole Nationale Supérieure Agronomique, 145 avenue de Muret, F-31076, Toulouse Cédex, France.
authors.forename: E
authors.initials: E
authors.lastname: Soler
authors.forename: G
authors.initials: G
authors.lastname: Feron
authors.forename: M
authors.initials: M
authors.lastname: Clastre
authors.forename: R
authors.initials: R
authors.lastname: Dargent
authors.forename: M
authors.initials: M
authors.lastname: Gleizes
authors.forename: C
authors.initials: C
authors.lastname: Ambid