title: Sulfur oxidation to sulfate coupled with electron transfer to electrodes by Desulfuromonas strain TZ1.
abstract: Microbial oxidation of elemental sulfur with an electrode serving as the electron acceptor is of interest because this may play an important role in the recovery of electrons from sulfidic wastes and for current production in marine benthic microbial fuel cells. Enrichments initiated with a marine sediment inoculum, with elemental sulfur as the electron donor and a positively poised (+300 mV versus Ag/AgCl) anode as the electron acceptor, yielded an anode biofilm with a diversity of micro-organisms, including Thiobacillus, Sulfurimonas, Pseudomonas, Clostridium and Desulfuromonas species. Further enrichment of the anode biofilm inoculum in medium with elemental sulfur as the electron donor and Fe(III) oxide as the electron acceptor, followed by isolation in solidified sulfur/Fe(III) medium yielded a strain of Desulfuromonas, designated strain TZ1. Strain TZ1 effectively oxidized elemental sulfur to sulfate with an anode serving as the sole electron acceptor, at rates faster than Desulfobulbus propionicus, the only other organism in pure culture previously shown to oxidize S° with current production. The abundance of Desulfuromonas species enriched on the anodes of marine benthic fuel cells has previously been interpreted as acetate oxidation driving current production, but the results presented here suggest that sulfur-driven current production is a likely alternative.
authors.affiliation: Department of Microbiology, University of Massachusetts, Amherst, MA, USA.
authors.forename: Tian
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Microbiology, University of Massachusetts, Amherst, MA, USA.
authors.forename: Timothy S
authors.initials: TS
authors.lastname: Bain
authors.affiliation: Department of Microbiology, University of Massachusetts, Amherst, MA, USA.
authors.forename: Melissa A
authors.initials: MA
authors.lastname: Barlett
authors.affiliation: Department of Microbiology, University of Massachusetts, Amherst, MA, USA.
authors.forename: Shabir A
authors.initials: SA
authors.lastname: Dar
authors.affiliation: Department of Microbiology, University of Massachusetts, Amherst, MA, USA.
authors.forename: Oona L
authors.initials: OL
authors.lastname: Snoeyenbos-West
authors.affiliation: Department of Microbiology, University of Massachusetts, Amherst, MA, USA.
authors.forename: Kelly P
authors.initials: KP
authors.lastname: Nevin
authors.affiliation: Department of Microbiology, University of Massachusetts, Amherst, MA, USA.
authors.forename: Derek R
authors.initials: DR
authors.lastname: Lovley
mesh_terms: D001674:Bioelectric Energy Sources; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D045287:Desulfuromonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004560:Electricity; D004566:Electrodes / Q000382:microbiology*; D019015:Geologic Sediments / Q000382:microbiology; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D017422:Sequence Analysis, DNA; D013431:Sulfates / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D013431:Sulfates; D013455:Sulfur