title: In vivo three dimensional dual wavelength photoacoustic tomography imaging of the far red fluorescent protein E2-Crimson expressed in adult zebrafish.
abstract: For the first time the far red fluorescent protein (FP) E2-Crimson genetically expressed in the exocrine pancreas of adult zebrafish has been non-invasively mapped in 3D in vivo using photoacoustic tomography (PAT). The distribution of E2-Crimson in the exocrine pancreas acquired by PAT was confirmed using epifluorescence imaging and histology, with optical coherence tomography (OCT) providing complementary structural information. This work demonstrates the depth advantage of PAT to resolve FP in an animal model and establishes the value of E2-Crimson for PAT studies of transgenic models, laying the foundation for future longitudinal studies of the zebrafish as a model of diseases affecting inner organs.
authors.affiliation: Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Währinger Gürtel 18-20, AKH 4L, 1090 Vienna, Austria.
authors.forename: Mengyang
authors.initials: M
authors.lastname: Liu
authors.forename: Nicole
authors.initials: N
authors.lastname: Schmitner
authors.forename: Michelle G
authors.initials: MG
authors.lastname: Sandrian
authors.forename: Behrooz
authors.initials: B
authors.lastname: Zabihian
authors.forename: Boris
authors.initials: B
authors.lastname: Hermann
authors.forename: Willi
authors.initials: W
authors.lastname: Salvenmoser
authors.forename: Dirk
authors.initials: D
authors.lastname: Meyer
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Drexler
keywords: (170.3880) Medical and biological imaging; (170.5120) Photoacoustic imaging; (170.6280) Spectroscopy, fluorescence and luminescence; (170.6960) Tomography