title: [Determination of zearalanol and related mycotoxins in pork by solid-phase extraction coupled with ultra performance liquid chromatography-tandem mass spectrometry].
abstract: A method was established for the determination of six compounds of zearalanol and related mycotoxins in pork and its products. After hydrolysis of the target compounds in the pork by beta-glucosidase/sulfatase, they were extracted with methanol aqueous solution and further purified by an HLB column. The separation was performed on a BEH C18 column with gradient elution using acetonitrile-water as mobile phases. The analytes were determined by mass spectrometry using electrospray ionization (ESI) in negative scan mode and multiple reaction monitoring (MRM) mode. alpha-Zearalenol-D7 and beta-zearalenol-D7 were used as internal standards. The good linearities (r > 0. 999) were achieved for the six compounds over the range of 1. 0 - 100 microg/L based on the internal standard calibration. The detection limits of the method were 0.03 -0.09 microg/kg. The mean recoveries of the six target compounds spiked at three levels from 1. 0 - 10. 0 microg/kg ranged from 76. 7% to 100. 5% with the relative standard deviations (RSDs) less than 20%. The proposed method is simple, sensitive, reproducible, and complies with the regulations for the determination of trace contaminant residues in food matrice.
authors.affiliation: Institute of Nutrition and Food Hygiene, Beijing Center for Disease Control &Prevention, Beijing 100013, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Li
authors.forename: Sai
authors.initials: S
authors.lastname: Fan
authors.forename: Rong
authors.initials: R
authors.lastname: Zhao
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Guohua
authors.initials: G
authors.lastname: Wu
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D009183:Mycotoxins / Q000032:analysis*; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D013552:Swine; D053719:Tandem Mass Spectrometry; D015029:Zeranol / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D009183:Mycotoxins; C029659:zearalenol; D015029:Zeranol