title: Efficient siRNA delivery and tumor accumulation mediated by ionically cross-linked folic acid-poly(ethylene glycol)-chitosan oligosaccharide lactate nanoparticles: for the potential targeted ovarian cancer gene therapy.
abstract: For effective ovarian cancer gene therapy, systemic administrated tumor-targeting siRNA/folic acid-poly(ethylene glycol)-chitosan oligosaccharide lactate (FA-PEG-COL) nanoparticles is vital for delivery to cancer site(s). siRNA/FA-PEG-COL nanoparticles were prepared by ionic gelation for effective FA receptor-expressing ovarian cancer cells transfection and in vivo accumulation. The chemical structure of FA-PEG-COL conjugate was characterized by MALDI-TOF-MS, FT-IR and (1)H NMR. The average size of siRNA/FA-PEG-COL nanoparticles was approximately 200 nm, and the surface charge was +8.4 mV compared to +30.5 mV with siRNA/COL nanoparticles. FA-PEG-COL nanoparticles demonstrated superior compatibility with erythrocytes in terms of degree of aggregation and haemolytic activity and also effects on cell viability was lower when compared with COL nanoparticles. FA grafting significantly facilitated the uptake of nanoparticles via receptor mediated endocytosis as demonstrated by flow cytometry. The in vitro transfection and gene knockdown efficiency of HIF-1α were superior to COL nanoparticles (76-62%, respectively) and was comparable to Lipofectamine 2000 (79%) as demonstrated by RT-qPCR and Western blot. Gene knockdown at the molecular level translated into effective inhibition of proliferation in vitro. Accumulation efficiency of FA-PEG-COL nanoparticles was investigated in BALB/c mice bearing OVK18 #2 tumor xenograft using in vivo imaging. The active targeting FA-PEG-COL nanoparticles showed significantly greater accumulation than the passive targeting COL nanoparticles. Based on the results obtained, siRNA/FA-PEG-COL nanoparticles show much potential for effective ovarian cancer treatment via gene therapy.
authors.affiliation: Department of Biochemistry, Kochi Medical School, Kochi University, Oko-Cho, Nankoku, Kochi 783-8505, Japan.
authors.forename: Tony Shing Chau
authors.initials: TS
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Kochi Medical School, Kochi University, Oko-Cho, Nankoku, Kochi 783-8505, Japan; Center for Innovative and Translational Medicine, Kochi Medical School, Kochi University, Oko-Cho, Nankoku, Kochi 783-8505, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Yawata
authors.affiliation: Department of Biochemistry, Kochi Medical School, Kochi University, Oko-Cho, Nankoku, Kochi 783-8505, Japan; Center for Innovative and Translational Medicine, Kochi Medical School, Kochi University, Oko-Cho, Nankoku, Kochi 783-8505, Japan. Electronic address: khonke@kochi-u.ac.jp.
authors.forename: Koichi
authors.initials: K
authors.lastname: Honke
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000008:administration & dosage / Q000737:chemistry; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D005260:Female; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000737:chemistry; D005492:Folic Acid / Q000737:chemistry; D055785:Gene Knockdown Techniques; D015316:Genetic Therapy / Q000379:methods; D006461:Hemolysis; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D010051:Ovarian Neoplasms / Q000235:genetics / Q000628:therapy*; D011092:Polyethylene Glycols / Q000737:chemistry; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: C569686:Alexa Fluor 647; D002232:Carbocyanines; D005456:Fluorescent Dyes; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D034741:RNA, Small Interfering; D011092:Polyethylene Glycols; D048271:Chitosan; D005492:Folic Acid
keywords: ANOVA; BCA; COL; CV; Chitosan; DCC; DCl; DEPC; DLS; DMEM; Dulbecco’s modified eagle’s medium; ECL; EDC; EPR; EtBr; FA; FA–PEG–COL; FBS; FT-IR; Folic acid; Fourier transformed infrared spectroscopy; HIF-1α; HRP; MALDI-TOF-MS; MWCO; N-(3-dimethylaminopropyl)-N-ethylcarbodiimide; N-hydroxysuccinimide; NH(2)–PEG–COOH; NHS; NIRF; NPs; NTC; Nanoparticles; Ovarian cancer; PDI; PVDF; RBCs; RGD peptide; RT-qPCR; TBE; TBS-T; TPP; Targeting; Tris-buffered saline with Tween; Tris/borate/EDTA; analysis of variance; arginine–glycine–aspartic acid peptide; bicinchoninic acid; centipoise; chitosan oligosaccharide lactate; coefficient of variation; cps; deuterium chloride; dicyclohexylcarbodiimide; diethyl pyrocarbonate; dynamic light scattering; enhanced chemiluminescence; enhanced permeability and retention effect; ethidium bromide; fetal bovine serum; folic acid; folic acid–poly(ethylene glycol)–chitosan oligosaccharide lactate; horseradish peroxidise; hypoxic inducible factor-1 alpha; matrix-assisted laser desorption/ionization-time of flight mass spectrometry; molecular weight cut off; nanoparticles; near-infrared fluorescence; no template control; polydispersity index; polyvinylidene fluoride; real-time quantitative polymerase chain reaction; red blood cells; siRNA; tripolyphosphate; α-aminopropyl-ω-carboxypentyloxy-polyxyethylene⋅hydrochloride