title: Control of olfactory ensheathing cell behaviors by electrospun silk fibroin fibers.
abstract: Olfactory ensheathing cells (OECs), the only glial cell type that normally penetrates the transition zone between the peripheral and central nervous systems, are one of the most promising candidates for cell transplantation in repairing spinal cord injury (SCI). However, we must manipulate and regulate OECs' behavior to make these cells effective in cell transplantation. In the present study, we assessed the response of rat OECs to different variants of nanofibrous silk fibroin mats with regard to cell morphology, adhesion, proliferation, and migration and the related gene and protein expression. Results showed that OECs adhere and spread more easily on Tussah silk fibroin (TSF) fibers than Bombyx mori silk fibroin fibers, resulting in a higher rate of cell proliferation and gene and protein expression, examined by RT-PCR and ELISA. In addition, the morphology of OECs on microfibers is mainly polygonal with short protrusions, whereas the OECs on nanofibers exhibit a bipolar shape with long protrusions that align along the fibers, especially when aligned fibers are employed. Moreover, OECs on silk fibroin nanofibers migrate more efficiently than those on poly-L-lysine (PLL). Based on the experimental results, the morphology, adhesion, spread, gene and protein expression, and migration of OECs could be modulated and regulated by adjusting the contents and structure of silk fibroin nanofibers, shedding light on the control of OECs' behavior in nerve tissue engineering and thus the future therapeutic intervention for nerve repair after injury. This manuscript is published as part of the International Association of Neurorestoratology (IANR) supplement issue of Cell Transplantation.
authors.forename: Zhihai
authors.initials: Z
authors.lastname: Fan
authors.forename: Yixin
authors.initials: Y
authors.lastname: Shen
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Baoqi
authors.initials: B
authors.lastname: Zuo
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lu
authors.forename: Peng
authors.initials: P
authors.lastname: Wu
authors.forename: Zonggang
authors.initials: Z
authors.lastname: Xie
authors.forename: Qirong
authors.initials: Q
authors.lastname: Dong
authors.forename: Huanxiang
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D012831:Bombyx; D048708:Cell Growth Processes / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D017690:Cell Transplantation / Q000379:methods*; D002478:Cells, Cultured; D005349:Fibroins / Q000737:chemistry*; D008297:Male; D008855:Microscopy, Electron, Scanning; D009416:Nerve Regeneration / Q000502:physiology*; D009830:Olfactory Bulb / Q000166:cytology* / Q000502:physiology / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005349:Fibroins