title: The effect of a gelatin β-tricalcium phosphate sponge loaded with mesenchymal stem cells (MSC), bone morphogenic protein-2, and platelet-rich plasma (PRP) on equine articular cartilage defect.
abstract: We evaluated the curative efficacy of a gelatin β-tricalcium phosphate (β-TCP) sponge loaded with mesenchymal stem cells (MSC), bone morphogenic protein-2 (BMP-2), and platelet-rich plasma (PRP) by insertion into an experimentally induced osteochondral defect. A hole of 10 mm diameter and depth was drilled in the bilateral medial femoral condyles of 7 thoroughbred horses, and into each either a loaded sponge (treatment) or a saline-infused β-TCP sponge (control) was inserted. After 16 weeks, defects were examined by computed tomography, macroscopic analyses, and histological analyses. The median subchondral bone density and macroscopic subscores for joint healing were significantly higher in the treatment legs (P < 0.05). Although there was no significant difference in total histological scores between groups, hyaline cartilaginous tissue was observed across a wider area in the treatment group. Equine joint healing can be enhanced by inserting a BMP-2-, MSC-, and PRP-impregnated β-TCP sponge at the lesion site.
authors.affiliation: Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro-city, Hokkaido, 080-8555, Japan (Tsuzuki, Seo, Yamada, Haneda, Sasaki); Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro-city, Hokkaido, 080-8555, Japan (Furuoka); and Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University, Sakyo-ku, Kyoto, 606-8507, Japan (Tabata).
authors.forename: Nao
authors.initials: N
authors.lastname: Tsuzuki
authors.forename: Jong-pil
authors.initials: JP
authors.lastname: Seo
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Yamada
authors.forename: Shingo
authors.initials: S
authors.lastname: Haneda
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Furuoka
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
authors.forename: Naoki
authors.initials: N
authors.lastname: Sasaki
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000737:chemistry*; D002357:Cartilage Diseases / Q000628:therapy / Q000662:veterinary; D002358:Cartilage, Articular / Q000293:injuries / Q000473:pathology; D005260:Female; D005781:Gelatin Sponge, Absorbable / Q000737:chemistry / Q000627:therapeutic use*; D006734:Horse Diseases / Q000628:therapy*; D006736:Horses; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods / Q000662:veterinary*; D053657:Platelet-Rich Plasma; D023822:Tissue Engineering; D016474:Weight-Bearing
chemical_list: D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate