title: Genetic revelation of hexavalent chromium toxicity using Caenorhabditis elegans as a biosensor.
abstract: The interaction of heavy metals such as hexavalent chromium, Cr (VI) with the environment drastically influences living organisms leading to an ecological imbalance. Caenorhabditis elegans, a saprophytic nematode having 60-80% homology with human genes offers a distinct advantage to be used as a biosensor for the appraisal of heavy metal-induced environmental toxicity and risk monitoring. The present study examines the toxicity effects of K2Cr2O7 as Cr (VI) on stress-related gene expression and morphometric parameters of C. elegans under in vitro conditions to identify genetic markers for environmental pollution. Alterations in growth and modified gene expression were observed in Cr (VI)-exposed N2 worms. The 24-h median lethal concentration for Cr (VI) was observed as 158.5 mgl(-1). Use of the responses of stress-related gene expression suggests that C. elegans can be used as an efficient biosensor for figuring out the precise route of Cr (VI)-induced environmental toxicity in a quick, simple, and inexpensive manner.
authors.affiliation: Microbial Technology and Nematology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh,India.
authors.forename: Shilpi Khare
authors.initials: SK
authors.lastname: Saikia
authors.affiliation: Microbial Technology and Nematology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh,India.
authors.forename: Rupali
authors.initials: R
authors.lastname: Gupta
authors.affiliation: Microbial Technology and Nematology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh,India.
authors.forename: Aakanksha
authors.initials: A
authors.lastname: Pant
authors.affiliation: Microbial Technology and Nematology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh,India.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Pandey
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D015374:Biosensing Techniques; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress