title: Elevated α-methyl-γ-hydroxy-1,N2-propano-2'-deoxyguanosine levels in urinary samples from individuals exposed to urban air pollution.
abstract: Acetaldehyde and crotonaldehyde are genotoxic aldehydes present in tobacco smoke and vehicle exhaust. The reaction of these aldehydes with 2'-deoxyguanosine in DNA produces α-methyl-γ-hydroxy-1,N(2)-propano-2'-deoxyguanosine (1,N(2)-propanodGuo). Online HPLC-tandem mass spectrometry was utilized to accurately quantify 1,N(2)-propanodGuo in human urinary samples from 47 residents of São Paulo City (SP) and 35 residents of the rural municipality of São João da Boa Vista (SJBV) in the state of São Paulo. Significantly higher 1,N(2)-propanodGuo levels were found in the samples from SP donors than in samples from SJBV donors. Our results provide the first evidence that elevated levels of 1,N(2)-propanodGuo in urinary samples may be correlated with urban air pollution.
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo , CEP 05508-000, São Paulo, Brazil.
authors.forename: Camila C M
authors.initials: CC
authors.lastname: Garcia
authors.forename: Florêncio P
authors.initials: FP
authors.lastname: Freitas
authors.forename: Angélica B
authors.initials: AB
authors.lastname: Sanchez
authors.forename: Paolo
authors.initials: P
authors.lastname: Di Mascio
authors.forename: Marisa H G
authors.initials: MH
authors.lastname: Medeiros
mesh_terms: D000079:Acetaldehyde / Q000737:chemistry; D000293:Adolescent; D000328:Adult; D000393:Air Pollutants / Q000737:chemistry* / Q000633:toxicity / Q000652:urine; D000447:Aldehydes / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry* / Q000187:drug effects; D018736:DNA Adducts / Q000302:isolation & purification / Q000652:urine*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000652:urine; D006801:Humans; D008297:Male; D008875:Middle Aged; D012907:Smoking; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D001335:Vehicle Emissions / Q000633:toxicity; D055815:Young Adult
chemical_list: D000393:Air Pollutants; D000447:Aldehydes; D018736:DNA Adducts; D001335:Vehicle Emissions; C501840:alpha-methyl-gamma-hydroxy-1,N2-propano-2'-deoxyguanosine; D004247:DNA; C012796:2-butenal; D003849:Deoxyguanosine; D000079:Acetaldehyde