title: Classical swine fever virus infection modulates serum levels of INF-α, IL-8 and TNF-α in 6-month-old pigs.
abstract: Several studies have highlighted the important role of cytokines in disease development of classical swine fever virus (CSFV) infection. In the present study, we examined the kinetics of 7 porcine cytokines in serum from pigs infected with 3 different CSFV strains. Based on the clinical picture in 6-month-old Danish pigs, the strains used for inoculation were classified as being of low (Bergen), low to moderate (Eystrup) and moderate to high (Lithuania) virulence. The cytokines interferon-alpha (INF-α), interleukin-8 (IL-8) and tumor necrosis factor-alpha (TNF-α) showed increased levels after CSFV infection with more or less comparable course in the 3 groups. However, the cytokine level peaked with a 2-3 days delay in pigs infected with the low virulent strain compared to those infected with a moderately or highly virulent strain. These findings may indicate that INF-α, IL-8 and TNF-α are involved in the immune response during CSFV infection with strains of different virulence.
authors.affiliation: Section for Virology, National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark; Section for Molecular Disease Biology, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlaegevej 88, 1, DK-1870 Frederiksberg C, Denmark.
authors.forename: T
authors.initials: T
authors.lastname: von Rosen
authors.forename: L
authors.initials: L
authors.lastname: Lohse
authors.forename: J
authors.initials: J
authors.lastname: Nielsen
authors.forename: Å
authors.initials: Å
authors.lastname: Uttenthal
mesh_terms: D000818:Animals; D006691:Classical Swine Fever / Q000097:blood / Q000503:physiopathology*; D006692:Classical Swine Fever Virus / Q000472:pathogenicity; D016898:Interferon-alpha / Q000097:blood*; D016209:Interleukin-8 / Q000097:blood*; D013552:Swine / Q000097:blood / Q000821:virology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*; D014774:Virulence / Q000502:physiology
chemical_list: D016898:Interferon-alpha; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha
keywords: Classical swine fever virus; Cytokines; Pathogenesis; Pestivirus; Pigs; Virulence