title: Liposome-coated hydrogel spheres: delivery vehicles with tandem release from distinct compartments.
abstract: We have fabricated unilamellar lipid bilayer VESicle-COated hydrogel spheres (VESCOgels) by carbodiimide-mediated coupling of liposomes bearing surface amines to core-shell hydrogel spheres bearing surface carboxyls. The amine-containing moiety, 3-O (2-aminoethoxyethyloxyethyl)carbamyl cholesterol (AECHO), was incorporated into large unilamellar vesicles (LUVs), diameter ∼100 nm, composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). The hydrogel, diameter ∼ 1 μm, consisted of a core of poly(N-isopropyl acrylamide) (pNIPAM) and a shell of p(NIPAM-co-acrylic acid (AA)). Activation of these surface-displayed carboxyls with N-hydroxysuccinimidyl (NHS) esters permitted amine coupling upon addition of AECHO-containing POPC LUVs. Bilayer integrity of the hydrogel-bound LUVs was maintained, and fusion of LUVs did not occur. Fluorescence assays of the release of cobalt-calcein trapped within hydrogel-bound LUVs and of sodium fluorescein trapped within the hydrogel itself showed that each compartment retained its distinct release attributes: fast release from the microgel and slow release from the LUVs. It is envisioned that VESCOgels will be useful, therefore, in applications requiring temporally controlled delivery of distinct drugs.
authors.affiliation: Department of Chemistry and ‡Department of Physics, University of Toronto , Toronto, Ontario, Canada.
authors.forename: Qasim
authors.initials: Q
authors.lastname: Saleem
authors.forename: Zhenfu
authors.initials: Z
authors.lastname: Zhang
authors.forename: Claudiu C
authors.initials: CC
authors.lastname: Gradinaru
authors.forename: Peter M
authors.initials: PM
authors.lastname: Macdonald
mesh_terms: D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D010316:Particle Size; D013499:Surface Properties
chemical_list: D004337:Drug Carriers; D008081:Liposomes; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate