title: Effect of concentrated growth factors on beagle periodontal ligament stem cells in vitro.
abstract: Identifying a reliable and effective cytokine or growth factor group has been the focus of stem cell osteogenic induction studies. Concentrated growth factors (CGFs) as the novel generation of platelet concentrate products, appear to exhibit a superior clinical and biotechnological application potential, however, there are few studies that have demonstrated this effect. This study investigated the proliferation and differentiation of periodontal ligament stem cells (PDLSCs) co‑cultured with CGFs. The rate of proliferation was analyzed by cell counting and an MTT assay. Mineralization nodule counts, alkaline phosphatase activity detection, qPCR, western blot analysis and immunohistochemistry were used to analyze mineralization effects. The results showed that CGF significantly promoted the proliferation of PDLSCs, and exhibited a dose‑dependent effect on the activation and differentiation of the stem cells. The application of CGF on PDLSC proliferation and osteoinduction may offer numerous clinical and biotechnological application strategies.
authors.affiliation: Center of Implant Dentistry, Hospital of Stomatology, Tongji University, Shanghai 200092, P.R. China.
authors.forename: Bohan
authors.initials: B
authors.lastname: Yu
authors.forename: Zuolin
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004285:Dogs; D058574:Integrin-Binding Sialoprotein / Q000235:genetics / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010513:Periodontal Ligament / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*
chemical_list: D058574:Integrin-Binding Sialoprotein; D036341:Intercellular Signaling Peptides and Proteins; D015675:Osteocalcin