title: Conversion of grass biomass into fermentable sugars and its utilization for medium chain length polyhydroxyalkanoate (mcl-PHA) production by Pseudomonas strains.
abstract: This study investigated the potential of grass biomass as a feedstock for mcl-PHA production. Pretreatments (2% NaOH at 120°C or hot water at 120°C) of perennial ryegrass were employed alone or in combination with sodium chlorite/acetic acid (SC/AA) delignification to evaluate the enzymatic digestibility and subsequent utilization of resultant sugars by Pseudomonas strains. NaOH pretreated sample had better digestibility than raw and hot water treated samples and this hydrolysate supported good growth of all tested strains with limited mcl-PHA (6-17% of cell dry mass (CDM)) accumulation. Digestibility of both untreated and pretreated samples was improved after SC/AA delignification and produced glucose (74-77%) rich hydrolysates. Tested strains accumulated 20-34% of CDM as PHA when these hydrolysates were used as sole carbon and energy source. CDM and PHA yields obtained for these strains when tested with laboratory grade sugars was similar to that achieved with grass derived sugars.
authors.affiliation: School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland; Technology Centre for Biorefining and Bioenergy, Orbsen Building, National University of Ireland, Galway, Ireland.
authors.forename: Reeta
authors.initials: R
authors.lastname: Davis
authors.forename: Rashmi
authors.initials: R
authors.lastname: Kataria
authors.forename: Federico
authors.initials: F
authors.lastname: Cerrone
authors.forename: Trevor
authors.initials: T
authors.lastname: Woods
authors.forename: Shane
authors.initials: S
authors.lastname: Kenny
authors.forename: Anthonia
authors.initials: A
authors.lastname: O'Donovan
authors.forename: Maciej
authors.initials: M
authors.lastname: Guzik
authors.forename: Hamid
authors.initials: H
authors.lastname: Shaikh
authors.forename: Gearoid
authors.initials: G
authors.lastname: Duane
authors.forename: Vijai Kumar
authors.initials: VK
authors.lastname: Gupta
authors.forename: Maria G
authors.initials: MG
authors.lastname: Tuohy
authors.forename: Ramesh Babu
authors.initials: RB
authors.lastname: Padamatti
authors.forename: Eoin
authors.initials: E
authors.lastname: Casey
authors.forename: Kevin E
authors.initials: KE
authors.lastname: O'Connor
mesh_terms: D018533:Biomass; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002241:Carbohydrates / Q000494:pharmacology; D004798:Enzymes / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D008129:Lolium / Q000187:drug effects / Q000378:metabolism*; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D011549:Pseudomonas / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D004798:Enzymes; D054813:Polyhydroxyalkanoates
keywords: Biomass; Enzyme hydrolyses; Perennial ryegrass; Pre-treatment; mcl-Polyhydroxyalkanoates