title: Changes induced by peripheral nerve injury in the morphology and nanomechanics of sensory neurons.
abstract: Peripheral nerve injury in vivo promotes a regenerative growth in vitro characterized by an improved neurite regrowth. Knowledge of the conditioning injury effects on both morphology and mechanical properties of live sensory neurons could be instrumental to understand the cellular and molecular mechanisms leading to this regenerative growth. In the present study, we use differential interference contrast microscopy, fluorescence microscopy, and atomic force microscopy (AFM) to show that conditioned axotomy, induced by sciatic nerve injury, does not increase somatic size of sensory neurons from adult mice lumbar dorsal root ganglia but promotes the appearance of longer and larger neurites and growth cones. AFM on live neurons is also employed to investigate changes in morphology and membrane mechanical properties of somas of conditioned neurons following sciatic nerve injury. Mechanical analysis of the soma allows distinguishing neurons having a regenerative growth from control ones, although they show similar shapes and sizes.
authors.affiliation: Université Montpellier 2, Laboratoire Charles Coulomb UMR 5221, F-34095, Montpellier, FrancebCentre National de la Recherche Scientifique, Laboratoire Charles Coulomb UMR 5221, F-34095, Montpellier, FrancecLaboratoire LVBPPE- Centre de Biotechnologie de Sfax- BP «1177» Route de Sidi MANSOUR - Sfax 3018-Tunisie.
authors.forename: Ouafa
authors.initials: O
authors.lastname: Benzina
authors.forename: Vivien
authors.initials: V
authors.lastname: Szabo
authors.forename: Olivier
authors.initials: O
authors.lastname: Lucas
authors.forename: Mari-belle
authors.initials: MB
authors.lastname: Saab
authors.forename: Thierry
authors.initials: T
authors.lastname: Cloitre
authors.forename: Frédérique
authors.initials: F
authors.lastname: Scamps
authors.forename: Csilla
authors.initials: C
authors.lastname: Gergely
authors.forename: Marta
authors.initials: M
authors.lastname: Martin
mesh_terms: D000199:Actins / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D019771:Axotomy; D001696:Biomechanical Phenomena; D005260:Female; D051379:Mice; D008856:Microscopy, Fluorescence; D008857:Microscopy, Interference; D009416:Nerve Regeneration; D059348:Peripheral Nerve Injuries / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D011984:Sensory Receptor Cells / Q000737:chemistry / Q000378:metabolism / Q000473:pathology*; D018709:Statistics, Nonparametric; D014404:Tubulin / Q000737:chemistry / Q000378:metabolism
chemical_list: D000199:Actins; D014404:Tubulin