title: Biochemical and mass spectrometric analysis of 3'-end methylation of piRNAs.
abstract: Piwi-interacting RNAs (piRNA) are fully modified by 2'-O-methylation at their 3'-termini. This terminal methylation is required to prevent 3'-nucleotide addition, which serves as a tag for destabilization. In this chapter, we describe biochemical and mass spectrometric analyses of 2'-O-methylation at 3'-termini of piRNAs.
authors.affiliation: Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Tokyo, Japan.
authors.forename: Takeo
authors.initials: T
authors.lastname: Suzuki
authors.forename: Kenjyo
authors.initials: K
authors.lastname: Miyauchi
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Sakaguchi
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008745:Methylation; D051379:Mice; D010084:Oxidation-Reduction; D034741:RNA, Small Interfering / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D013737:Testis / Q000378:metabolism; D014462:Ultrafiltration
chemical_list: D034741:RNA, Small Interfering