title: Genetic validity of RAPD markers at the intra- and inter-specific level in wild Brassica species with n=9.
abstract: The sequence homology of co-migrating RAPD markers within a genus, across species, and among populations of a species was investigated. DNA was isolated from ten wild Brassica species with n=9 and the RAPD patterns were established using three random primers. Five RAPD markers which appeared to be characteristic for the n=9 species (genus level), four markers which appeared to be species specific, and one population-specific marker were isolated from agarose gels and hybridized to the RAPD profiles of the ten Brassica species. Two RAPD markers were cloned for comparison with gel-isolated RAPD fragment probes in hybridization experiments. Non-specific and background hybridization, occurring when gel-isolated fragments were used as probes, disappeared when cloned fragments were used. A total of 250 RAPD-marker hybridizations were scored according to visual presence or absence in a gel lane. All except three markers hybridized as expected, resulting in an error rate of 1.2%. The deviating results included a lack of hybridization although a band was visible in the gel, a length polymorphism for one marker, and a dual hybridization signal for two single-band markers.
authors.affiliation: Department of Plant Breeding Research, Swedish University of Agricultural Sciences, S-268 31, Svalöv, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Lannér
authors.forename: T
authors.initials: T
authors.lastname: Bryngelsson
authors.forename: M
authors.initials: M
authors.lastname: Gustafsson