title: Construction of a filamentous phage display peptide library.
abstract: The concept of phage display is based on insertion of random oligonucleotides at an appropriate location within a structural gene of a bacteriophage. The resulting phage will constitute a library of random peptides displayed on the surface of the bacteriophages, with the encoding genotype packaged within each phage particle. Using a phagemid/helper phage system, the random peptides are interspersed between wild-type coat proteins. Libraries of phage-expressed peptides may be used to search for novel peptide ligands to target proteins. The success of finding a peptide with a desired property in a given library is highly dependent on the diversity and quality of the library. The protocols in this chapter describe the construction of a high-diversity library of phagemid vector encoding fusions of the phage coat protein pVIII with random peptides, from which a phage library displaying random peptides can be prepared.
authors.affiliation: Department of Pharmaceutical Biosciences, University of Oslo, Oslo, Norway.
authors.forename: Annette
authors.initials: A
authors.lastname: Fagerlund
authors.forename: Astrid Hilde
authors.initials: AH
authors.lastname: Myrset
authors.forename: Mari Ann
authors.initials: MA
authors.lastname: Kulseth
mesh_terms: D001483:Base Sequence; D005822:Genetic Vectors / Q000378:metabolism; D017912:Inovirus / Q000378:metabolism*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000378:metabolism; D019151:Peptide Library; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014170:Transformation, Genetic
chemical_list: D009841:Oligonucleotides; D019151:Peptide Library