title: In vivo volumetric photoacoustic molecular angiography and therapeutic monitoring with targeted plasmonic nanostars.
abstract: Photoacoustic (PA) imaging promises deeper tissue penetration while maintaining rich optical contrast as compared to other high resolution optical imaging techniques. In this report, a near-infrared pulse laser serves as the excitation source, and 128 ultrasonic transducers are spirally distributed on a hemispherical surface to receive PA signals for three-dimensional (3D) image reconstruction. With these attributes, the unique modality produces an isotropic and homogeneous spatial resolution (∼200 μm) with penetration depth of centimeters. Cyclic Arg-Gly-Asp (RGD) peptides conjugated plasmonic gold nanostars (RGD-GNS) are designed to specifically target over-expressed integrin α(v)β₃ on tumor neovasculature, enabling highly sensitive angiography and photothermal therapy (PTT). After the administration of RGD-GNS, tumor angiogenesis is clearly imaged with enhanced contrast, and the growth of tumor is effectively inhibited by PTT after laser irradiation. This study suggest that the PA angiography with plasmonic RGD-GNS can be applied as a triple functional platform for tumor diagnosis, PTT, and treatment monitoring. This PA technique offers deeper imaging depth with homogeneous resolution over existing optical imaging techniques for early diagnosis of tumor angiogenesis as well as on-the-spot nanotherapeutic evaluation.
authors.affiliation: Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB) National Institutes of Health (NIH), Bethesda, MD, 20892, USA; Center for Molecular Imaging and Translational Medicine School of Public Health, Xiamen University, Xiamen, 361005, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Nie
authors.forename: Shouju
authors.initials: S
authors.lastname: Wang
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Wang
authors.forename: Pengfei
authors.initials: P
authors.lastname: Rong
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: Huang
authors.forename: Guangming
authors.initials: G
authors.lastname: Lu
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Chen
mesh_terms: D000792:Angiography / Q000379:methods*; D000818:Animals; D045744:Cell Line, Tumor; D003287:Contrast Media; D005910:Glioma / Q000098:blood supply / Q000175:diagnosis / Q000628:therapy; D006046:Gold; D006801:Humans; D006979:Hyperthermia, Induced; D021621:Imaging, Three-Dimensional; D039302:Integrin alphaVbeta3 / Q000378:metabolism; D053768:Metal Nanoparticles / Q000648:ultrastructure; D051379:Mice; D008819:Mice, Nude; D009389:Neovascularization, Pathologic / Q000175:diagnosis*; D009842:Oligopeptides; D019047:Phantoms, Imaging; D061088:Photoacoustic Techniques / Q000379:methods*
chemical_list: D003287:Contrast Media; D039302:Integrin alphaVbeta3; D009842:Oligopeptides; D006046:Gold; C047981:arginyl-glycyl-aspartic acid
keywords: photoacoustic imaging; photothermal therapy; plasmonic nanostars; therapy monitoring; tumor angiogenesis