title: Protective effect of omega-3 fatty acid against mercury chloride intoxication in mice.
abstract: The aim of this study was to investigate the protective effect of omega-3 fatty acid in HgCI2 toxicity in mice. Levels of malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NO) and total sialic acid (TSA), and histopathological changes in selected organs were evaluated. Twenty-eight mice were equally divided into 4 groups, namely Groups I-IV. Group I animals received intraperitoneal (ip) injection of physiological saline solution; Group II animals received ip injection of 0.4mg/kg/day HgCI2; Group III animals received ip injection of 0.4mg/kg/day HgCI2 in addition to subcutaneous (sc) injection of 0.5g/kg/day omega-3 fatty acid; and Group IV animals received sc injection of 0.5g/kg/day omega-3 fatty acid. All treatments lasted 7 days. The levels of MDA, NO and TSA were significantly higher in Group II and lower in Groups III and IV as compared to the Group I. GSH level was the highest in Group IV. In histopathology, severe degeneration in liver and kidney was observed in Group II animals. These degrading changes were seen to be reduced greatly in Group III animals. The results suggested that omega-3 fatty acid might attenuate HgCI2-induced toxicity by improving antioxidant status and acute phase response in mice.
authors.affiliation: Department of Biochemistry, Faculty of Veterinary Medicine, Kafkas University, 36100 Kars, Turkey.
authors.forename: Mahmut
authors.initials: M
authors.lastname: Karapehlivan
authors.affiliation: Department of Biochemistry, Faculty of Veterinary Medicine, Kafkas University, 36100 Kars, Turkey.
authors.forename: Metin
authors.initials: M
authors.lastname: Ogun
authors.affiliation: Department of Molecular Biology and Genetic, Faculty of Arts and Science, Kafkas University, 36100 Kars, Turkey. Electronic address: inankaya_@hotmail.com.
authors.forename: Inan
authors.initials: I
authors.lastname: Kaya
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, Kafkas University, 36100 Kars, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Ozen
authors.affiliation: Atatürk Health Services Vocational School, Kafkas University, 36100 Kars, Turkey.
authors.forename: Haci Ahmet
authors.initials: HA
authors.lastname: Deveci
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, Kafkas University, 36100 Kars, Turkey.
authors.forename: Musa
authors.initials: M
authors.lastname: Karaman
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D008627:Mercuric Chloride / Q000633:toxicity*; D051379:Mice; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015525:Fatty Acids, Omega-3; D009569:Nitric Oxide; D008315:Malondialdehyde; D008627:Mercuric Chloride; D005978:Glutathione; D019158:N-Acetylneuraminic Acid
keywords: Malondialdehyde; Mercury chloride; Nitric oxide; Omega-3 fatty acid; Sialic acid