title: Changes in energy-regulated molecules in the trophocytes and fat cells of young and old worker honeybees (Apis mellifera).
abstract: Trophocytes and fat cells of honeybees (Apis mellifera) have been used for cellular senescence studies, but the changes in the expression, concentration, and activity of cellular energy-regulated molecules that occur with aging in worker bees is unknown. In this study, energy-regulated molecules were evaluated in the trophocytes and fat cells of young and old workers. The results showed that (i) adenosine monophosphate-activated protein kinase-α2 (AMPK-α2) expression increased with aging, whereas phosphorylated AMPK-α2 expression, the phosphorylated AMPK/AMPK ratio, and AMPK activity decreased with aging; (ii) adenosine diphosphate and adenosine triphosphate concentrations decreased with aging, the AMP concentration was unchanged, the adenosine diphosphate/adenosine triphosphate ratio did not change with aging, and the AMP/adenosine triphosphate ratio increased with aging; (iii) the cyclic AMP concentration decreased with aging, and cyclic AMP-specific phosphodiesterases activity increased with aging; (iv) silent information regulator 2 (Sir2) expression increased with aging, whereas its activity decreased with aging; and (v) peroxisome proliferator-activated receptor-α expression decreased with aging. These results show that the trophocytes and fat cells of young workers have higher cellular energy status and express higher levels of energy-regulated molecules than those of old workers and that aging results in a decline in the energy status of trophocytes and fat cells in worker honeybees.
authors.affiliation: Department of Biomedical Sciences and Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan. hsu@mail.cgu.edu.tw.
authors.forename: Chin-Yuan
authors.initials: CY
authors.lastname: Hsu
authors.affiliation: Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan.
authors.forename: Yu-Lung
authors.initials: YL
authors.lastname: Chuang
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D001516:Bees / Q000166:cytology* / Q000378:metabolism; D016922:Cellular Senescence / Q000502:physiology*; D000242:Cyclic AMP / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology; D006801:Humans; D019476:Insect Proteins / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology; D047493:PPAR alpha / Q000378:metabolism; D010766:Phosphorylation; D037761:Sirtuins / Q000378:metabolism
chemical_list: D019476:Insect Proteins; D047493:PPAR alpha; D000242:Cyclic AMP; D055372:AMP-Activated Protein Kinases; D037761:Sirtuins
keywords: ADP; AMP; AMPK; ATP; Aging; Honeybee.; PPAR-α; Sir2