title: Combined genetic and physiological analysis of a locus contributing to quantitative variation.
abstract: The natural variation of many traits is controlled by multiple genes, individually referred to as quantitative trait loci (QTL), that interact with the environment to determine the ultimate phenotype of any individual. A QTL has yet to be described molecularly, in part because strategies to systematically identify them are underdeveloped and because the subtle nature of QTLs prevents the application of standard methods of gene identification. Therefore, it will be necessary to develop a systematic approach(es) for the identification of QTLs based upon the numerous positional data now being accumulated through molecular marker analyses. We have characterized a QTL by the following three-step approach: (1) identification of a QTL in complex populations, (2) isolation and genetic mapping of this QTL in near-isogenic lines, and (3) identification of a candidate gene using map position and physiological criteria. Using this approach we have characterized a plant height QTL in maize that maps to chromosome 9 near the centromere. Both map position and physiological criteria suggest the gibberillin biosynthesis gene dwarf3 as a candidate gene for this QTL.
authors.affiliation: Department of Plant Sciences, University of Arizona, 85721, Tucson, AZ, USA.
authors.forename: P
authors.initials: P
authors.lastname: Touzet
authors.forename: R G
authors.initials: RG
authors.lastname: Winkler
authors.forename: T
authors.initials: T
authors.lastname: Helentjaris