title: Netilmicin-induced modulation of melanogenesis in HEMa-LP melanocytes.
abstract: It is known that aminoglycoside antibiotics bind well to melanin biopolymer, but the relation between their affinity to melanin and ototoxicity is not well established. The aim of this work was to examine the impact of netilmicin on melanogenesis in cultured normal human melanocytes (HEMa-LP). The WST-1 assay was used to detect netilmicin cytotoxic effect. Netilmicin induced concentration-dependent loss in melanocytes viability. The value of EC50 was found to be - 75.0 mM. The analyzed antibiotic inhibited melanin biosynthesis in a concentration-dependent manner. Increasing the drug concentration resulted also in a decrease of cellular tyrosinase activity. Netilmicin-induced modulation of melanogenesis in melanocytes in vitro may explain a potential role of melanin and melanocytes in the mechanisms of aminoglycosides ototoxic effects in vivo.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University of Silesia, Jagiellońska 4, PL-41-200 Sosnowiec, Poland. dwrzesniok@sum.edu.pl
authors.forename: Dorota
authors.initials: D
authors.lastname: Wrześniok
authors.forename: Artur
authors.initials: A
authors.lastname: Beberok
authors.forename: Michał
authors.initials: M
authors.lastname: Otreba
authors.forename: Ewa
authors.initials: E
authors.lastname: Buszman
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008543:Melanins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D008544:Melanocytes / Q000187:drug effects*; D014442:Monophenol Monooxygenase / Q000378:metabolism; D009428:Netilmicin / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D008543:Melanins; D009428:Netilmicin; D014442:Monophenol Monooxygenase