title: Label-free immunosensor based on Pd nanoplates for amperometric immunoassay of alpha-fetoprotein.
abstract: In this paper, Pd nanoplates were used as a kind of electrode materials for fabrication of an electrochemical immunosensor, which was applied for detection of cancer biomarker alpha-fetoprotein (AFP). Thanks to the unique structure and properties of Pd nanoplates, the antibody of AFP (Ab) was effectively immobilized onto the surface of the Pd nanoplates modified glassy carbon electrode (GCE). Moreover, the good electrochemical properties of Pd nanoplates greatly improved the electronic transmission rate and enhanced the electrochemical signal, which led to an increase of the detection sensitivity. Based on the specific antibody-antigen interaction, a label-free immunosensor based on Pd nanoplates was developed for sensing of AFP. The current method allows us to detect AFP over a wide concentration range from 0.01 to 75.0 ng/mL with a detection limit of 4 pg/mL. The proposed immunosensor has been used to determine AFP in human serum with satisfactory results.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.forename: He
authors.initials: H
authors.lastname: Li
authors.forename: Yihe
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
authors.forename: Hongmin
authors.initials: H
authors.lastname: Ma
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Du
mesh_terms: D000906:Antibodies / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry; D000509:alpha-Fetoproteins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000906:Antibodies; D037742:Nanotubes, Carbon; D000509:alpha-Fetoproteins; D010165:Palladium; D006046:Gold
keywords: Alpha-fetoprotein; Cancer biomarker; Immunosensor; Label-free; Palladium nanoplates