title: Prosthecobacter algae sp. nov., isolated from activated sludge using algal metabolites.
abstract: A Gram-stain-negative, fusiform-shaped, facultatively anaerobic bacterial strain, designated EBTL04(T), was isolated from activated sludge using algal metabolites and taxonomically characterized through polyphasic investigation. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain EBTL04(T) belongs to the family Verrucomicrobiaceae, class Verrucomicrobiae, and is closely related to Prosthecobacter dejongeii DSM 12251(T) (98.6 % sequence similarity), Prosthecobacter fusiformis ATCC 25309(T) (97.9 %), Prosthecobacter debontii DSM 14044(T) (97.5%), Prosthecobacter vanneervenii DSM 12252(T) (94.7%) and Prosthecobacter fluviatilis KCTC 22182(T) (93.7%). The G+C content of the genomic DNA of strain EBTL04(T) was 62.7 mol%. The menaquinone MK-6 was detected as the predominant quinone. Strain EBTL04(T) contained phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine as major polar lipids. A fatty acid profile with C(16 : 1)ω5c, iso-C(14 : 0), C(16 : 0), anteiso-C(15 : 0) and C(14 : 0) as the major components supported the classification of strain EBTL04(T) in the genus Prosthecobacter. Based on several phenotypic, genotypic and chemotaxonomic features, strain EBTL04(T) was clearly differentiated from its phylogenetic neighbours. Therefore, strain EBTL04(T) should be considered to represent a novel species of the genus Prosthecobacter, for which the name Prosthecobacter algae sp. nov. is proposed. The type strain is EBTL04(T) ( = KCTC 23681(T) = JCM 18053(T)).
authors.affiliation: School of Civil and Environmental Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.
authors.forename: Jangho
authors.initials: J
authors.lastname: Lee
authors.affiliation: School of Civil and Environmental Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.
authors.forename: Banghyo
authors.initials: B
authors.lastname: Park
authors.affiliation: School of Civil and Environmental Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.
authors.forename: Sung-Geun
authors.initials: SG
authors.lastname: Woo
authors.affiliation: School of Civil and Environmental Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.
authors.forename: Juyoun
authors.initials: J
authors.lastname: Lee
authors.affiliation: School of Civil and Environmental Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.
authors.forename: Joonhong
authors.initials: J
authors.lastname: Park
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D058086:Microalgae / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D061327:Verrucomicrobia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D018508:Water Purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D024482:Vitamin K 2; C042455:menaquinone 6