title: On-chip processing of particles and cells via multilaminar flow streams.
abstract: The processing of particles, cells, and droplets for reactions, analyses, labeling, and coating is an important aspect of many microfluidic workflows. However, performing multi-step processes is typically a laborious and time-consuming endeavor. By exploiting the laminar nature of flow within microchannels, such procedures can benefit in terms of both speed and simplicity. This can be achieved either by manipulating the flow streams around the objects of interest, particularly for the localized perfusion of cells, or by manipulating the objects themselves within the streams via a range of forces. Here, we review the variety of methods that have been employed for performing such "multilaminar flow" procedures on particles, cells, and droplets.
authors.affiliation: Department of Chemistry, The University of Hull, Cottingham Road, Hull, HU6 7RX, UK.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Tarn
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Lopez-Martinez
authors.forename: Nicole
authors.initials: N
authors.lastname: Pamme
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000166:cytology / Q000502:physiology; D001696:Biomechanical Phenomena / Q000502:physiology*; D002448:Cell Adhesion; D004058:Diffusion; D005057:Eukaryotic Cells / Q000166:cytology / Q000502:physiology; D006801:Humans; D060526:Magnetic Fields; D046210:Microfluidic Analytical Techniques; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D052898:Optical Tweezers; D010316:Particle Size; D012212:Rheology / Q000379:methods*