title: Co-metabolic biodegradation of acetamiprid by Pseudoxanthomonas sp. AAP-7 isolated from a long-term acetamiprid-polluted soil.
abstract: An AAP-degrading bacterium, AAP-7, was isolated from AAP-polluted soil. AAP-7 was identified as Pseudoxanthomonas sp. on the basis of the comparative analysis of 16S rDNA sequences. The strain was able to transformate more than 80% AAP by means of co-metabolism and degraded AAP via hydrolysis or demethylation to form (E)-3-(((6-chloropyridin-3yl)methyl)(methyl)amino)acrylonitrile and N-((6-chloropyridin-3yl)methyl)-N-methylprop-1-en-2-amine, both of which transformed into ultimate product, which was 1-(6-chloropyridin-3yl)-N-methylmethanamine. A novel degradation pathway was proposed based on these metabolites. AAP could be transformed with a maximum specific degradation rate, half-saturation constant and inhibit constant of 1.775/36 h, 175.3 mg L(-1), and 396.5 mg L(-1), respectively, which proved that the degradation rate of AAP could be restrained at high AAP concentration. This paper highlights a significant potential use of co-metabolic cultures of microbial cells for the cleanup of AAP-contaminated soil.
authors.affiliation: College of Life Sciences, Huaibei Normal University, Huaibei 235000, PR China. Electronic address: wanf-3344@163.com.
authors.forename: Guangli
authors.initials: G
authors.lastname: Wang
authors.forename: Yanjiao
authors.initials: Y
authors.lastname: Zhao
authors.forename: Hao
authors.initials: H
authors.lastname: Gao
authors.forename: Wenlong
authors.initials: W
authors.lastname: Yue
authors.forename: Minghua
authors.initials: M
authors.lastname: Xiong
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Ge
mesh_terms: D000704:Analysis of Variance; D001483:Base Sequence; D001673:Biodegradation, Environmental; D002244:Carbon / Q000032:analysis; D004275:DNA, Ribosomal / Q000235:genetics; D007700:Kinetics; D008969:Molecular Sequence Data; D000073943:Neonicotinoids; D009584:Nitrogen / Q000032:analysis; D010802:Phylogeny; D011549:Pseudomonas / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D011725:Pyridines / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013997:Time Factors
chemical_list: D004275:DNA, Ribosomal; D000073943:Neonicotinoids; D011725:Pyridines; D012989:Soil Pollutants; C464485:acetamiprid; D002244:Carbon; D009584:Nitrogen
keywords: AAP; Acetamiprid; C; CCD; Co-metabolism; Kinetics; MM; MM supplemented with acetamiprid; MM-A; MM-A supplemented with glucose; MM-AG; Mineral salts medium; N; PDA; Percentage of degradated acetamiprid; Response surface methodology; acetamiprid; central composite design; the carbon source; the nitrogen source