title: Enhancement of chitosanase production by cell immobilization of Gongronella sp. JG.
abstract: Chitosanase production of Gongronella sp. JG cells immobilized in calcium alginate gel and polyurethane foam was compared with that of the free cells, there was a 60% increase in the enzyme yield (2429 U/L) compared to the highest yield obtained from free cells (1513 U/L). The optimal immobilization parameters (concentrations of sodium alginate, calcium chloride, bead inoculums, bead diameter, etc) for the enhanced production of chitosanase were determined as: sodium alginate 2% (w/v), 0.1 M calcium chloride, inoculum 10 mL beads to 100 mL production media and 2.7 mm bead diameter. Maximum chitosanase production was achieved with initial pH of 5.5 and temperature of 30 °C. The alginate beads had well stability, retained 85% ability of enzyme production even after 7 cycles of repeated batch fermentation. These results showed the immobilization technique was a feasible and economical method for chitosansase production by Gongronella sp. JG.
authors.affiliation: Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Hefei, Anhui Province, P.R. China. ; School of Life Science, Anhui University, Hefei, Anhui Province, P.R. China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Mingli
authors.initials: M
authors.lastname: Tang
keywords: Gongronella sp. JG; cell immobilization; chitosanase production; sodium alginate