title: Application of an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum.
abstract: This study presents an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum. A new solvent system composed of petroleum ether/ethyl acetate/water (4:2:1, v/v/v) was developed for the liquid-liquid extraction of the crude extract from R. tanguticum. As a result, emodin, aloe-emodin, physcion, and chrysophanol were greatly enriched in the organic layer. In addition, an efficient method was successfully established to separate and purify the above anthraquinones by high-speed counter-current chromatography and preparative HPLC. This study supplies a new alternative method for the rapid enrichment, separation, and purification of emodin, aloe-emodin, physcione, and chrysophanol.
authors.affiliation: Key Laboratory of Tibetan medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P. R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Yongling
authors.initials: Y
authors.lastname: Liu
authors.forename: Denglang
authors.initials: D
authors.lastname: Zou
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Jinmao
authors.initials: J
authors.lastname: You
authors.forename: Guoying
authors.initials: G
authors.lastname: Zhou
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Yulin
authors.initials: Y
authors.lastname: Li
mesh_terms: D000880:Anthraquinones / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003377:Countercurrent Distribution / Q000379:methods*; D059625:Liquid-Liquid Extraction / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D012250:Rheum / Q000737:chemistry*
chemical_list: D000880:Anthraquinones; D010936:Plant Extracts
keywords: Anthraquinones; High-speed counter-current chromatography; Liquid-liquid extraction; Preparative HPLC; Rheum tanguticum