title: Ottowia shaoguanensis sp. nov., isolated from coking wastewater.
abstract: A Gram-negative, short rod-shaped, floc-forming bacterial strain J5-66(T) without any flagellum was isolated from coking wastewater collected from Shaoguan, Guangdong, China. It was capable of optimal growth at pH 7, 30 °C, and 1-2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belonged to the genus Ottowia in Comamonadaceae, and the highest 16S rRNA gene sequence similarity was 96.2 % with Ottowia pentelensis DSM 21699(T). The major cellular fatty acids of strain J5-66(T) were C₁₆:₁ω7c/C₁₆:₁ ω6c (45.0 %), C₁₆:₀ (21.1 %), C₁₈:₁ ω7c or/and C₁₈:₁ ω6c (19.2 %). The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, glycolipid and two unidentified phospholipids (PL1 and PL2). The predominant ubiquinone was Q-8, and the G+C content of the genome DNA was 64.4 mol%. On the basis of genetic, phenotypic and chemotaxonomic analyses, strain J5-66(T) represents a novel species of the genus Ottowia for which the name Ottowia shaoguanensis sp. nov. is proposed. The type strain is J5-66(T) (=CGMCC 1.12431(T) =LMG 27408(T)).
authors.affiliation: College of Engineering, Peking University, Beijing, 100871, People's Republic of China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Geng
authors.forename: Xin-Chi
authors.initials: XC
authors.lastname: Pan
authors.forename: Ran
authors.initials: R
authors.lastname: Mei
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Wang
authors.forename: Ji-Quan
authors.initials: JQ
authors.lastname: Sun
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Liu
authors.forename: Yue-Qin
authors.initials: YQ
authors.lastname: Tang
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Wu
mesh_terms: D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D042621:Comamonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D062065:Wastewater / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D062065:Wastewater