title: Image analysis of chromatin remodelling.
abstract: Chromatin packaging plays a significant role in regulating gene transcription. Study of the higher-order packing states of chromatin by image analysis at the light microscope level, especially when validated by methods of molecular biology, immunochemistry, and/or immunocytochemistry, enabled the detection of changes involved in the processes associated with or preceding alterations in transcriptional activities. Here, we recommend and describe the use of relatively simple methods for staining and detecting chromatin remodelling by image analysis.
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, University of Campinas, Campinas, SP, Brazil.
authors.forename: Benedicto de Campos
authors.initials: Bde C
authors.lastname: Vidal
authors.forename: Marina B
authors.initials: MB
authors.lastname: Felisbino
authors.forename: Maria Luiza S
authors.initials: ML
authors.lastname: Mello
mesh_terms: D000818:Animals; D002843:Chromatin; D042002:Chromatin Assembly and Disassembly; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D051379:Mice; D015591:Microspectrophotometry; D041681:NIH 3T3 Cells; D010641:Phenotype; D013194:Staining and Labeling
chemical_list: D002843:Chromatin