title: Actin network disassembly powers dissemination of Listeria monocytogenes.
abstract: Several bacterial pathogens hijack the actin assembly machinery and display intracellular motility in the cytosol of infected cells. At the cell cortex, intracellular motility leads to bacterial dissemination through formation of plasma membrane protrusions that resolve into vacuoles in adjacent cells. Here, we uncover a crucial role for actin network disassembly in dissemination of Listeria monocytogenes. We found that defects in the disassembly machinery decreased the rate of actin tail turnover but did not affect the velocity of the bacteria in the cytosol. By contrast, defects in the disassembly machinery had a dramatic impact on bacterial dissemination. Our results suggest a model of L. monocytogenes dissemination in which the disassembly machinery, through local recycling of the actin network in protrusions, fuels continuous actin assembly at the bacterial pole and concurrently exhausts cytoskeleton components from the network distal to the bacterium, which enables membrane apposition and resolution of protrusions into vacuoles.
authors.affiliation: Department of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale School of Medicine, New Haven, CT 06536, USA.
authors.forename: Arthur M
authors.initials: AM
authors.lastname: Talman
authors.forename: Ryan
authors.initials: R
authors.lastname: Chong
authors.forename: Jonathan
authors.initials: J
authors.lastname: Chia
authors.forename: Tatyana
authors.initials: T
authors.lastname: Svitkina
authors.forename: Hervé
authors.initials: H
authors.lastname: Agaisse
mesh_terms: D008841:Actin Cytoskeleton / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D000199:Actins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism / Q000382:microbiology; D051338:Cofilin 1 / Q000235:genetics / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism / Q000382:microbiology; D005786:Gene Expression Regulation; D020904:Glia Maturation Factor / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D008089:Listeria monocytogenes / Q000502:physiology*; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014617:Vacuoles / Q000378:metabolism / Q000382:microbiology
chemical_list: D000199:Actins; C495810:CFL1 protein, human; D051338:Cofilin 1; D020904:Glia Maturation Factor; D008840:Microfilament Proteins; D034741:RNA, Small Interfering; C118067:WDR1 protein, human
keywords: AIP1; ARP2/3; Actin assembly; Actin network disassembly; CFL1; GMFB; Listeria; WDR1