title: Outer envelope membranes from chloroplasts are isolated as right-side-out vesicles.
abstract: Outer envelope membranes were isolated from purified chloroplasts of pea leaves. The sidedness of the vesicles was analyzed by (i) aqueous polymer-two phase partitioning, (ii) the effect of limited proteolysis on the outer-envelope proteins (OEP) 86 and OEP 7 in intact organelles and isolated membranes, (iii) fluorescence-microscopy and finally (iv) binding of precursor polypeptides to isolated outer-membrane vesicles. The results demonstrate that purified outer envelope membranes occur largely (>90%) as right-side-out vesicles.
authors.affiliation: Botanisches Institut, Universität Kiel, Olshausenstrasse 40, W-2300, Kiel, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Waegemann
authors.forename: S
authors.initials: S
authors.lastname: Eichacker
authors.forename: J
authors.initials: J
authors.lastname: Soll