title: Rapid characterisation and comparison of saponin profiles in the seeds of Korean Leguminous species using ultra performance liquid chromatography with photodiode array detector and electrospray ionisation/mass spectrometry (UPLC-PDA-ESI/MS) analysis.
abstract: The present work was reported on investigation of saponin profiles in nine different legume seeds, including soybean, adzuki bean, cowpea, common bean, scarlet runner bean, lentil, chick pea, hyacinth bean, and broad bean using ultra performance liquid chromatography with photodiode array detector and electrospray ionisation/mass spectrometry (UPLC-PDA-ESI/MS) technique. A total of twenty saponins were characterised under rapid and simple conditions within 15min by the 80% methanol extracts of all species. Their chemical structures were elucidated as soyasaponin Ab (1), soyasaponin Ba (2), soyasaponin Bb (3), soyasaponin Bc (4), soyasaponin Bd (5), soyasaponin αg (6), soyasaponin βg (7), soyasaponin βa (8), soyasaponin γg (9), soyasaponin γa (10), azukisaponin VI (11), azukisaponin IV (12), azukisaponin II (13), AzII (14), AzIV (15), lablaboside E (16), lablaboside F (17), lablaboside D (18), chikusetusaponin IVa (19), and lablab saponin I (20). The individual and total saponin compositions exhibited remarkable differences in all legume seeds. In particular, soyasaponin βa (8) was detected the predominant composition in soybean, cowpea, and lentil with various concentrations. Interestingly, soybean, adzuki bean, common bean, and scarlet runner bean had high saponin contents, while chick pea and broad bean showed low contents.
authors.affiliation: Department of Functional Crop, National Institute of Crop Science (NICS), Rural Development Administration, Miryang 627-803, Republic of Korea.
authors.forename: Tae Joung
authors.initials: TJ
authors.lastname: Ha
authors.forename: Byong Won
authors.initials: BW
authors.lastname: Lee
authors.forename: Ki Hun
authors.initials: KH
authors.lastname: Park
authors.forename: Seong Hun
authors.initials: SH
authors.lastname: Jeong
authors.forename: Hyun-Tae
authors.initials: HT
authors.lastname: Kim
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Ko
authors.forename: In-Youl
authors.initials: IY
authors.lastname: Baek
authors.forename: Jin Hwan
authors.initials: JH
authors.lastname: Lee
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D007887:Fabaceae / Q000737:chemistry* / Q000145:classification; D015394:Molecular Structure; D012503:Saponins / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry* / Q000145:classification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D012503:Saponins
keywords: Legumes; Methanol extract; Saponin; Seeds; Soyasaponin βg; UPLC–PDA–ESI/MS