title: Optimization of endoglucanase and xylanase activities from Fusarium verticillioides for simultaneous saccharification and fermentation of sugarcane bagasse.
abstract: Enzymatic hydrolysis is an important but expensive step in the production of ethanol from biomass. Thus, the production of efficient enzymatic cocktails is of great interest for this biotechnological application. The production of endoglucanase and xylanase activites from F. verticillioides were optimized in a factorial design (2(5)) followed by a CCDR design. Endoglucanase and xylanase activities increased from 2.8 to 8.0 U/mL and from 13.4 to 114 U/mL, respectively. The optimal pH and temperature were determined for endoglucanase (5.6, 80 °C), cellobiase (5.6, 60 °C), FPase (6.0, 55 °C) and xylanase (7.0, 50 °C). The optimized crude extract was applied in saccharification and fermentation of sugarcane bagasse from which 9.7 g/L of ethanol was produced at an ethanol/biomass yield of 0.19.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil.
authors.forename: Maíra N
authors.initials: MN
authors.lastname: de Almeida
authors.forename: Valéria M
authors.initials: VM
authors.lastname: Guimarães
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Falkoski
authors.forename: Guilherme B T
authors.initials: GB
authors.lastname: Paes
authors.forename: José Ivo
authors.initials: JI
authors.lastname: Ribeiro
authors.forename: Evan M
authors.initials: EM
authors.lastname: Visser
authors.forename: Rafael F
authors.initials: RF
authors.lastname: Alfenas
authors.forename: Olinto L
authors.initials: OL
authors.lastname: Pereira
authors.forename: Sebastião T
authors.initials: ST
authors.lastname: de Rezende
mesh_terms: D018533:Biomass; D002480:Cellulase / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D005285:Fermentation; D005670:Fusarium / Q000201:enzymology*; D006868:Hydrolysis; D031786:Saccharum / Q000737:chemistry
chemical_list: D000431:Ethanol; D002482:Cellulose; C027433:bagasse; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases