title: Identification of chrysanthemum cultivars and stability of DNA fingerprint patterns.
abstract: Several techniques of DNA analysis were applied to identify chrysanthemum cultivars. Unrelated cultivars could be distinguished by using RAPDs (random amplified polymorphic DNAs), inter-SSR (simple sequence repeat) PCR (polymerase chain reaction), hybridization-based DNA fingerprinting, as well as RFLPs (restriction fragment length polymorphisms). Cultivars with different flower colours and belonging to one family, i.e. vegetatively derived from 1 cultivar, appeared to have the same DNA fragment patterns, whichever technique was applied. The absence of polymorphisms between different accessions of the same cultivar indicated a high stability of the observed patterns.
authors.affiliation: Institute of Evolutionary and Ecological Sciences, University of Leiden, PO Box 9516, 2300, RA Leiden, the Netherlands.
authors.forename: K
authors.initials: K
authors.lastname: Wolff
authors.forename: E
authors.initials: E
authors.lastname: Zietkiewicz
authors.forename: H
authors.initials: H
authors.lastname: Hofstra