title: [The effect of Ca2+ on the properties of the large conductance cation channels of the nuclear envelope of the cerebellar neurons].
abstract: Previously we have found the large conductance cation channels (LCCC) in the nuclear membranes, where inositol-1,4,5-triphosphate receptors (IP3Rs) were also observed. Probably IP3Rs and LCCC are functionally connected: LCCC may provide the counterflow of K+, which prevent the formation of the negative potential in the lumen of the nuclear envelope and in such way may prolong the Ca2+ releasing by IP3Rs. LCCC are poorly studied and their molecular nature is still unknown. We investigated the effect of Ca2+ on properties of these channels. Our results demonstrated the main biophysical properties of LCCC changed significantly neither in Ca(2+)-free solution, nor with high concentrations of Ca2+ in the nuclear lumen. So, the level of Ca2+ repletion of the store does not influence the activity of LCCC.
authors.forename: O V
authors.initials: OV
authors.lastname: Lun'ko
authors.forename: O A
authors.initials: OA
authors.lastname: Fedorenko
authors.forename: S M
authors.initials: SM
authors.lastname: Marchenko
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D020013:Calcium Signaling; D002458:Cell Fractionation; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000378:metabolism; D051036:Large-Conductance Calcium-Activated Potassium Channels / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D009685:Nuclear Envelope / Q000187:drug effects* / Q000378:metabolism; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D053496:Inositol 1,4,5-Trisphosphate Receptors; D051036:Large-Conductance Calcium-Activated Potassium Channels; D011188:Potassium; D002118:Calcium