title: Cloning and identification of a DNA fragment coding for the sup1 gene of Saccharomyces cerevisiae.
abstract: A plasmid, pYsup1-1, containing a DNA fragment able to suppress the recessive mutant phenotype of the suppressor locus sup1 (allele sup1-ts36) of Saccharomyces cerevisiae was isolated from a bank of yeast chromosomal DNA cloned in cosmid p3030. The complementing gene was localized on a 2.6 kb DNA fragment by further subcloning. Evidence is presented that the cloned DNA segment codes for the sup1 structural gene (chromosome IIR).
authors.affiliation: Lehrstuhl für Mikrobiologie der Universität München, Maria-Ward-Straße 1a, D-8000, München 19, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Breining
authors.forename: A P
authors.initials: AP
authors.lastname: Surguchov
authors.forename: W
authors.initials: W
authors.lastname: Piepersberg