title: Gaussia luciferase-based mycoplasma detection assay in mammalian cell culture.
abstract: Mycoplasma contamination in mammalian cell culture is a common problem with serious consequences on experimental data, and yet many laboratories fail to perform regular testing. In this chapter, we describe a simple and sensitive mycoplasma detection assay based on the bioluminescent properties of the Gaussia luciferase reporter.
authors.affiliation: Experimental Therapeutics and Molecular Imaging Laboratory, Department of Neurology, Neuroscience Center, Massachusetts General Hospital, Boston, MA, USA.
authors.forename: M Hannah
authors.initials: MH
authors.lastname: Degeling
authors.forename: M Sarah S
authors.initials: MS
authors.lastname: Bovenberg
authors.forename: Marie
authors.initials: M
authors.lastname: Tannous
authors.forename: Bakhos A
authors.initials: BA
authors.lastname: Tannous
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D033342:Copepoda / Q000201:enzymology* / Q000235:genetics; D003470:Culture Media; D057809:HEK293 Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008163:Luminescent Measurements / Q000379:methods*; D009174:Mycoplasma / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D003470:Culture Media; D011994:Recombinant Proteins; D008156:Luciferases