title: Analysis of cationic amino acid transport activity in canine lens epithelial cells.
abstract: Cationic amino acid transport activity in a canine lens epithelial cells (LEC) line was investigated. The transporter activity of arginine was 0.424 ± 0.047 nmol/mg protein min, while the presence of N-ethylmaleimide, an inhibitor of the canine cationic amino acid transporter (CAT), reduced transport activity by 30%. A full-length cDNA sequence of canine CAT1 was 2558 bp long and was predicted to encode the 629 amino acid polypeptides. The deduced amino acid sequence of canine CAT1 showed similarities of 92.1% and 88.6% to those of the human and mouse, respectively. Western blot analysis detected a band at 70 kDa in a membrane protein sample of LEC. RT-PCR analysis confirmed that CAT1 was ubiquitously detected in all tissues examined.
authors.affiliation: Research Institute of Biosciences, Azabu University, School of Veterinary Medicine, 1-17-71 Fuchinobe, Chuou-ku, Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Hideharu
authors.initials: H
authors.lastname: Ochiai
authors.forename: Jun
authors.initials: J
authors.lastname: Moriyama
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kanemaki
authors.forename: Reiichiro
authors.initials: R
authors.lastname: Sato
authors.forename: Ken
authors.initials: K
authors.lastname: Onda
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000378:metabolism*; D001483:Base Sequence; D001692:Biological Transport / Q000235:genetics*; D027201:Cationic Amino Acid Transporter 1 / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs; D004847:Epithelial Cells / Q000378:metabolism*; D005033:Ethylmaleimide / Q000494:pharmacology; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology*; D008297:Male; D051379:Mice
chemical_list: D027201:Cationic Amino Acid Transporter 1; D018076:DNA, Complementary; D001120:Arginine; D005033:Ethylmaleimide