title: Assessment of the role in protection and pathogenesis of the Chlamydia muridarum V-type ATP synthase subunit A (AtpA) (TC0582).
abstract: A novel Chlamydia muridarum antigen (TC0582) was used to vaccinate BALB/c mice. Mice were also immunized with other components of the ATP synthase complex (TC0580, TC0581, and TC0584), or with the major outer membrane protein (MOMP). TC0582 was also formulated in combination with TC0580, TC0581 or MOMP. TC0582 alone, or in combination with the other antigens, elicited strong Chlamydia-specific humoral and cellular immune responses. Vaccinated animals were challenged intranasally and the course of the infection was followed for 10 days. Based on percentage change in body weight, lung weight, and number of Chlamydia inclusion forming units recovered from the lungs, mice immunized with TC0582, TC0581 or MOMP, as single antigens, showed significant protection. Mice immunized with combinations of two antigens were also protected but the level of protection was not additive. TC0582 has sequence homology with the eukaryotic ATP synthase subunit A (AtpA). Therefore, to determine if immunization with TC0582, or with Chlamydia, elicited antibodies that cross-reacted with the mouse AtpA, the two proteins were printed on a microarray. Sera from mice immunized with TC0582 and/or live Chlamydia, strongly reacted with TC0582 but did not recognize the mouse AtpA. In conclusion, TC0582 may be considered as a Chlamydia vaccine candidate.
authors.affiliation: Department of Pathology and Laboratory Medicine Medical Sciences I, Room D440 University of California, Irvine Irvine, California 92697-4800.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Cheng
authors.affiliation: Department of Pathology and Laboratory Medicine Medical Sciences I, Room D440 University of California, Irvine Irvine, California 92697-4800.
authors.forename: Pooja
authors.initials: P
authors.lastname: Jain
authors.affiliation: Department of Pathology and Laboratory Medicine Medical Sciences I, Room D440 University of California, Irvine Irvine, California 92697-4800.
authors.forename: Sukumar
authors.initials: S
authors.lastname: Pal
authors.affiliation: Department of Pathology and Laboratory Medicine Medical Sciences I, Room D440 University of California, Irvine Irvine, California 92697-4800.
authors.forename: Delia
authors.initials: D
authors.lastname: Tifrea
authors.affiliation: Department of Pathology and Laboratory Medicine Medical Sciences I, Room D440 University of California, Irvine Irvine, California 92697-4800.
authors.forename: Guifeng
authors.initials: G
authors.lastname: Sun
authors.affiliation: ImmPORT Therapeutics, Inc./Antigen Discovery Inc. 1 Technology Dr., Suite E309 Irvine, CA 92618.
authors.forename: Andy A
authors.initials: AA
authors.lastname: Teng
authors.affiliation: ImmPORT Therapeutics, Inc./Antigen Discovery Inc. 1 Technology Dr., Suite E309 Irvine, CA 92618.
authors.forename: Xiaowu
authors.initials: X
authors.lastname: Liang
authors.affiliation: ImmPORT Therapeutics, Inc./Antigen Discovery Inc. 1 Technology Dr., Suite E309 Irvine, CA 92618.|Department of Medicine 3052 Hewitt Hall University of California, Irvine Irvine, California 92697-4068.
authors.forename: Philip L
authors.initials: PL
authors.lastname: Felgner
authors.affiliation: Department of Pathology and Laboratory Medicine Medical Sciences I, Room D440 University of California, Irvine Irvine, California 92697-4800.
authors.forename: Luis M
authors.initials: LM
authors.lastname: de la Maza
mesh_terms: D025181:ATP Synthetase Complexes / Q000276:immunology*; D000818:Animals; D058491:Bacterial Load; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D001835:Body Weight; D002690:Chlamydia Infections / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D023502:Chlamydia muridarum / Q000201:enzymology* / Q000276:immunology*; D005260:Female; D008168:Lung / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D018410:Pneumonia, Bacterial / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D021122:Protein Subunits / Q000276:immunology; D037521:Virulence Factors / Q000276:immunology*
chemical_list: D001428:Bacterial Vaccines; D021122:Protein Subunits; D037521:Virulence Factors; D025181:ATP Synthetase Complexes
keywords: ATP synthase subunit A; Chlamydia; Immunization; Mice; Vaccine