title: Application of two microsatellite sequences in wheat storage proteins as molecular markers.
abstract: In eukaryotes, tandem arrays of simple-sequence repeat sequences can find applications as highly variable and multi-allelic PCR-based genetic markers. In hexaploid bread wheat, a large-genome inbreeding species with low levels of RFLP, di- and trinucleotide tandem repeats were found in 22 published gene sequences, two of which were converted to PCR-based markers. These were shown to be genome-specific and displayed high levels of variation. These characteristics make them especially suitable for intervarietal breeding applications.
authors.affiliation: Cambridge Laboratory, John Innes Centre, Norwich Research Park, NR4 7UH, Colney, Norwich, UK.
authors.forename: K M
authors.initials: KM
authors.lastname: Devos
authors.forename: G J
authors.initials: GJ
authors.lastname: Bryan
authors.forename: A J
authors.initials: AJ
authors.lastname: Collins
authors.forename: P
authors.initials: P
authors.lastname: Stephenson
authors.forename: M D
authors.initials: MD
authors.lastname: Gale