title: Cyclic nucleotide-gated channel subunit glycosylation regulates matrix metalloproteinase-dependent changes in channel gating.
abstract: Cyclic-nucleotide gated (CNG) channels are essential for phototransduction within retinal photoreceptors. We have demonstrated previously that the enzymatic activity of matrix metalloproteinase-2 and -9, members of the matrix metalloproteinase (MMP) family of extracellular, Ca(2+)- and Zn(2+)-dependent proteases, enhances the ligand sensitivity of both rod (CNGA1 and CNGB1) and cone (CNGA3 and CNGB3) CNG channels. Additionally, we have observed a decrease in the maximal CNG channel current (Imax) that begins late during MMP-directed gating changes. Here we demonstrate that CNG channels become nonconductive after prolonged MMP exposure. Concurrent with the loss of conductive channels is the increased relative contribution of channels exhibiting nonmodified gating properties, suggesting the presence of a subpopulation of channels that are protected from MMP-induced gating effects. CNGA subunits are known to possess one extracellular core glycosylation site, located at one of two possible positions within the turret loop near the pore-forming region. Our results indicate that CNGA glycosylation can impede MMP-dependent modification of CNG channels. Furthermore, the relative position of the glycosylation site within the pore turret influences the extent of MMP-dependent proteolysis. Glycosylation at the site found in CNGA3 subunits was found to be protective, while glycosylation at the bovine CNGA1 site was not. Relocating the glycosylation site in CNGA1 to the position found in CNGA3 recapitulated CNGA3-like protection from MMP-dependent processing. Taken together, these data indicate that CNGA glycosylation may protect CNG channels from MMP-dependent proteolysis, consistent with MMP modification of channel function having a requirement for physical access to the extracellular face of the channel.
authors.affiliation: Program in Neuroscience, Department of Integrative Physiology and Neuroscience, ‡WWAMI Medical Education Program, and §Center for Integrated Biotechnology, Washington State University , P.O. Box 647620, Pullman, Washington 99164, United States.
authors.forename: Starla E
authors.initials: SE
authors.lastname: Meighan
authors.forename: Peter C
authors.initials: PC
authors.lastname: Meighan
authors.forename: Elizabeth D
authors.initials: ED
authors.lastname: Rich
authors.forename: R Lane
authors.initials: RL
authors.lastname: Brown
authors.forename: Michael D
authors.initials: MD
authors.lastname: Varnum
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D054815:Cyclic Nucleotide-Gated Cation Channels / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D015640:Ion Channel Gating / Q000187:drug effects*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism; D016415:Sequence Alignment; D014982:Xenopus laevis
chemical_list: C518243:CNGA1 protein, human; C518238:CNGA3 protein, human; C415904:CNGB3 protein, human; D054815:Cyclic Nucleotide-Gated Cation Channels; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9