title: Overexpression of FXYD-3 is involved in the tumorigenesis and development of esophageal squamous cell carcinoma.
abstract: OBJECTIVE
  To investigate the association of FXYD-3 expression with clinicopathological variables and PINCH in patients with ESCC.
  
  
  METHODS
  Expression of FXYD-3 protein was immunohistochemically examined in normal esophageal mucous (n = 20) and ESCC (n = 64).
  
  
  RESULTS
  Expression of FXYD-3 in the cytoplasm markedly increased from normal esophageal epithelial cells to primary ESCC (P = 0.001). The expression of FXYD-3 was correlated with TNM stages and depth of tumor invasion. Furthermore, the cases with lymph node metastasis tended to show a higher frequency of positive expression than those without metastasis (P = 0.086), and FXYD-3 expression tended to be positively related to the expression of PINCH (P = 0.063). Moreover, the cases positive for both proteins had the highest frequency of lymph node metastasis (P = 0.001). However, FXYD-3 expression was not correlated with patient's gender (P = 0.847), age (P = 0.876), tumor location (P = 0.279), size (P = 0.771), grade of differentiation (P = 0.279), and survival (P = 0.113).
  
  
  CONCLUSIONS
  Overexpression of FXYD-3 in the cytoplasm may play an important role in the tumorigenesis and development in the human ESCC, particularly in combination with PINCH expression.
authors.affiliation: Department of Pathology, The First Hospital of Hebei Medical University, Shijiazhuang 050031, China ; Graduate School of Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Zhen-Long
authors.initials: ZL
authors.lastname: Zhu
authors.forename: Bao-Yong
authors.initials: BY
authors.lastname: Yan
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Yang
authors.forename: Ming-Wei
authors.initials: MW
authors.lastname: Wang
authors.forename: Hanswalter
authors.initials: H
authors.lastname: Zentgraf
authors.forename: Xiang-Hong
authors.initials: XH
authors.lastname: Zhang
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Sun
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D063646:Carcinogenesis / Q000235:genetics / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D016022:Case-Control Studies; D004938:Esophageal Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C091777:FXYD3 protein, human; D008565:Membrane Proteins; D009363:Neoplasm Proteins