title: The effect of Pediococcus acidilactici bacteria used as probiotic supplement on the growth and non-specific immune responses of green terror, Aequidens rivulatus.
abstract: A 56-day feeding trial was conducted on a species of ornamental fish called green terror (Aequidens rivulatus) (0.388 ± 0.0021 g) to assess the effect of probiotic bacteria, Pediococcus acidilactici on the growth indices and innate immune response. The fish were randomly allocated into 9 oval tanks (120 l) at a density of 60 fish per tank. The experimental diets were comprised of the control (C), C complemented with fish oil (O) and the probiotic and fish oil (PA) and fed ad lib twice a day. The growth indices (specific growth rate (SGR), feed conversion ratio (FCR) and immunological indices of fish fed the diets including lysozyme activity, total immunoglobulin and alternative complement activity were measured. The Fish fed with the diet containing P. acidilactici (PA) displayed significantly (P < 0.05) higher final weight (3.25 ± 0.065 g), weight gain (830.94 ± 9.46%), SGR (3.53 ± 0.02%/day) and lower FCR (1.45 ± 0.011) compared to those of other experimental diets. Total immunoglobulin (10.05 ± 0.12 μg/ml), lysozyme activity (4.08 ± 0.85 μg/ml) and alternative complement activity (2.65 ± 0.12 U/ml) in the serum of PA fed fish showed significant compared to other treatments (P < 0.05). The results showed positive effects of P. acidilactici as a potent probiotic on growth indices and non-specific immune system of green terror.
authors.affiliation: Department of Fisheries Sciences, Faculty of Natural Resources, University of Tehran, 331585-4314 Karaj, Iran. Electronic address: Neissi.alireza@ut.ac.ir.
authors.forename: Alireza
authors.initials: A
authors.lastname: Neissi
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Rafiee
authors.forename: Mohammadali
authors.initials: M
authors.lastname: Nematollahi
authors.forename: Omid
authors.initials: O
authors.lastname: Safari
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D023681:Cichlids / Q000254:growth & development* / Q000276:immunology*; D003170:Complement Pathway, Alternative / Q000187:drug effects; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements / Q000032:analysis; D005395:Fish Oils / Q000378:metabolism; D029941:Fish Proteins / Q000097:blood / Q000378:metabolism; D007113:Immunity, Innate; D007136:Immunoglobulins / Q000097:blood; D009113:Muramidase / Q000097:blood / Q000378:metabolism; D010376:Pediococcus / Q000737:chemistry*; D019936:Probiotics / Q000008:administration & dosage / Q000494:pharmacology*; D011897:Random Allocation
chemical_list: D005395:Fish Oils; D029941:Fish Proteins; D007136:Immunoglobulins; D009113:Muramidase
keywords: Green terror; Growth; Immunological indices; Pediococcus acidilactici