title: Agrobacterium tumefaciens-mediated transformation of Artemisia annua L. and regeneration of transgenic plants.
abstract: A transformation system was developed for Artemisia annua L. plants. Leaf explants from in vitro grown plants developed callus and shoots on medium with 0.05 mg/L naphthaleneacetic acid and 0.5 mg/L N(6)-benzyladenine after transformation with the C58C1 Rif(R) (pGV2260) (pTJK136) Agrobacterium tumefaciens strain. A concentration of 20 mg/L kanamycin was added in order to select transformed tissue. Kanamycin resistant shoots were rooted on naphthaleneacetic acid 0.1 mg/L. Polymerase chain reactions and DNA sequencing of the amplification products revealed that 75% of the regenerants contained the foreign genes. 94% of the transgenic plants showed a β-glucuronidase-positive response.
authors.affiliation: Laboratorium voor Farmaceutische Biotechnologie, Universiteit Gent, Harelbekestraat 72, B-9000, Gent, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Vergauwe
authors.forename: R
authors.initials: R
authors.lastname: Cammaert
authors.forename: D
authors.initials: D
authors.lastname: Vandenberghe
authors.forename: C
authors.initials: C
authors.lastname: Genetello
authors.forename: D
authors.initials: D
authors.lastname: Inze
authors.forename: M
authors.initials: M
authors.lastname: Van Montagu
authors.forename: E
authors.initials: E
authors.lastname: Van den Eeckhout