title: SOFI-based 3D superresolution sectioning with a widefield microscope.
abstract: BACKGROUND
  Fluorescence-based biological imaging has been revolutionized by the recent introduction of superresolution microscopy methods. 3D superresolution microscopy, however, remains a challenge as its implementation by existing superresolution methods is non-trivial.
  
  
  METHODS
  Here we demonstrate a facile and straightforward 3D superresolution imaging and sectioning of the cytoskeletal network of a fixed cell using superresolution optical fluctuation imaging (SOFI) performed on a conventional lamp-based widefield microscope.
  
  
  CONCLUSIONS
  SOFI's inherent sectioning capability effectively transforms a conventional widefield microscope into a superresolution 'confocal widefield' microscope.
authors.affiliation: Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, USA ; SOFast GmbH, Dresdener Str 14, 10999 Berlin, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Dertinger
authors.forename: Jianmin
authors.initials: J
authors.lastname: Xu
authors.forename: Omeed Foroutan
authors.initials: OF
authors.lastname: Naini
authors.forename: Robert
authors.initials: R
authors.lastname: Vogel
authors.forename: Shimon
authors.initials: S
authors.lastname: Weiss