title: A chemical perspective on transcriptional fidelity: dominant contributions of sugar integrity revealed by unlocked nucleic acids.
abstract: Transcription unlocked: A synthetic chemical biology approach involving unlocked nucleic acids was used to dissect the contribution of sugar backbone integrity to the RNA Polymerase II (Pol II) transcription process. An unexpected dominant role for sugar-ring integrity in Pol II transcriptional efficiency and fidelity was revealed.
authors.affiliation: Skaggs School of Pharmacy and Pharmaceutical Sciences, The University of California, San Diego, La Jolla, CA 92093-0625 (USA).
authors.forename: Liang
authors.initials: L
authors.lastname: Xu
authors.forename: Steven W
authors.initials: SW
authors.lastname: Plouffe
authors.forename: Jenny
authors.initials: J
authors.lastname: Chong
authors.forename: Jesper
authors.initials: J
authors.lastname: Wengel
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
mesh_terms: D055162:Biocatalysis; D002241:Carbohydrates / Q000737:chemistry*; D007700:Kinetics; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism*; D012319:RNA Polymerase II / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D014158:Transcription, Genetic
chemical_list: D002241:Carbohydrates; D009841:Oligonucleotides; C477371:locked nucleic acid; D012319:RNA Polymerase II
keywords: RNA polymerase II; nucleic acid analogues; sugar integrity; synthetic biology; transcriptional fidelity