title: Interleukin-17F single-nucleotide polymorphism (7488T>C) and its association with susceptibility to leprosy.
abstract: The objective of this study was to investigate the association, if any, between the interleukin-17F (7488T>C) (rs763780) polymorphism and susceptibility to leprosy and to elucidate the relationship between IL-17F genotypes and clinical profile of the disease. DNA was extracted from the peripheral venous blood of leprosy cases (n = 140), which were classified as per WHO classification into paucibacillary (PB) (n = 53) and multibacillary (MB) (n = 87) categories and healthy controls (n = 84) without any signs and symptoms of leprosy. The IL-17F (7488 T/C) polymorphism was genotyped using amplification refractory mutation system - polymerase chain reaction (Allele-specific amplification). In both PB and MB categories of leprosy cases, the homozygous TT genotype frequency was significantly higher than that of the healthy controls (78.70% vs. 29.76%, P < 0.05). The heterozygous TC genotype was higher in the controls than in the leprosy cases (57.14% vs. 17.68%, P < 0.05). TT genotype was more associated with the type 1 reactional states and tuberculoid/borderline tuberculoid groups in leprosy than the TC genotype. This study reveals that the IL-17F (7488T>C) single-nucleotide polymorphism is associated with susceptibility to leprosy and polymorphism confers decrease in risk of contracting leprosy in the north Indian cohort.
authors.affiliation: Stanley Browne Research Laboratory, The Leprosy Mission Community Hospital, New Delhi, India.
authors.forename: V S
authors.initials: VS
authors.lastname: Chaitanya
authors.forename: R S
authors.initials: RS
authors.lastname: Jadhav
authors.forename: M
authors.initials: M
authors.lastname: Lavania
authors.forename: M
authors.initials: M
authors.lastname: Singh
authors.forename: V
authors.initials: V
authors.lastname: Valluri
authors.forename: U
authors.initials: U
authors.lastname: Sengupta
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002648:Child; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007194:India; D020381:Interleukin-17 / Q000235:genetics*; D007918:Leprosy / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
chemical_list: C440021:IL17F protein, human; D020381:Interleukin-17