title: Time-course changes in immunoreactivities of glucokinase and glucokinase regulatory protein in the gerbil hippocampus following transient cerebral ischemia.
abstract: Glucose is a main energy source for normal brain functions. Glucokinase (GK) plays an important role in glucose metabolism as a glucose sensor, and GK activity is modulated by glucokinase regulatory protein (GKRP). In this study, we examined the changes of GK and GKRP immunoreactivities in the gerbil hippocampus after 5 min of transient global cerebral ischemia. In the sham-operated-group, GK and GKRP immunoreactivities were easily detected in the pyramidal neurons of the stratum pyramidale of the hippocampus. GK and GKRP immunoreactivities in the pyramidal neurons were distinctively decreased in the hippocampal CA1 region (CA), not CA2/3, 3 days after ischemia-reperfusion (I-R). Five days after I-R, GK and GKRP immunoreactivities were hardly detected in the CA1, not CA2/3, pyramidal neurons; however, at this point in time, GK and GKRP immunoreactivities were newly expressed in astrocytes, not microglia, in the ischemic CA1. In brief, GK and GKRP immunoreactivities are changed in pyramidal neurons and newly expressed in astrocytes in the ischemic CA1 after transient cerebral ischemia. These indicate that changes of GK and GKRP expression may be related to the ischemia-induced neuronal damage/death.
authors.affiliation: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea.
authors.forename: Joon Ha
authors.initials: JH
authors.lastname: Park
authors.forename: Choong Hyun
authors.initials: CH
authors.lastname: Lee
authors.forename: In Hye
authors.initials: IH
authors.lastname: Kim
authors.forename: Ji Hyeon
authors.initials: JH
authors.lastname: Ahn
authors.forename: Jeong-Hwi
authors.initials: JH
authors.lastname: Cho
authors.forename: Bing Chun
authors.initials: BC
authors.lastname: Yan
authors.forename: Jae-Chul
authors.initials: JC
authors.lastname: Lee
authors.forename: Tae Hun
authors.initials: TH
authors.lastname: Lee
authors.forename: Jeong Yeol
authors.initials: JY
authors.lastname: Seo
authors.forename: Jun Hwi
authors.initials: JH
authors.lastname: Cho
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
authors.forename: Il-Jun
authors.initials: IJ
authors.lastname: Kang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005849:Gerbillinae; D005941:Glucokinase / Q000378:metabolism*; D006624:Hippocampus / Q000201:enzymology / Q000378:metabolism*; D002546:Ischemic Attack, Transient / Q000201:enzymology / Q000378:metabolism*; D008297:Male
chemical_list: D002352:Carrier Proteins; C080906:glucokinase regulatory protein; D005941:Glucokinase