title: Overexpression of a partial fragment of the salt-responsive gene OsNUC1 enhances salt adaptation in transgenic Arabidopsis thaliana and rice (Oryza sativa L.) during salt stress.
abstract: The rice (Oryza sativa L.) nucleolin gene, OsNUC1, transcripts were expressed in rice leaves, flowers, seeds and roots but differentially expressed within and between two pairs of salt-sensitive and salt-resistant rice lines when subjected to salt stress. Salt-resistant lines exhibited higher OsNUC1 transcript expression levels than salt-sensitive lines during 0.5% (w/v) NaCl salt stress for 6d. Two sizes of OsNUC1 full-length cDNA were found in the rice genome database and northern blot analysis confirmed their existence in rice tissues. The longer transcript (OsNUC1-L) putatively encodes for a protein with a serine rich N-terminal, RNA recognition motifs in the central domain and a glycine- and arginine-rich repeat in the C-terminal domain, while the shorter one (OsNUC1-S) putatively encodes for the similar protein without the N-terminus. Without salt stress, OsNUC1-L expressing Arabidopsis thaliana Atnuc1-L1 plants displayed a substantial but incomplete revertant phenotype, whereas OsNUC1-S expression only induced a weak effect. However, under 0.5% (w/v) NaCl salt stress they displayed a higher relative growth rate, longer root length and a lower H2O2 level than the wild type plants, suggesting a higher salt resistance. Moreover, they displayed elevated AtSOS1 and AtP5CS1 transcript levels. We propose that OsNUC1-S plays an important role in salt resistance during salt stress, a new role for nucleolin in plants.
authors.affiliation: Biological Sciences Program, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Environmental and Plant Physiology Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Siriporn
authors.initials: S
authors.lastname: Sripinyowanich
authors.forename: Nontalee
authors.initials: N
authors.lastname: Chamnanmanoontham
authors.forename: Thanikarn
authors.initials: T
authors.lastname: Udomchalothorn
authors.forename: Somporn
authors.initials: S
authors.lastname: Maneeprasopsuk
authors.forename: Panudda
authors.initials: P
authors.lastname: Santawee
authors.forename: Teerapong
authors.initials: T
authors.lastname: Buaboocha
authors.forename: Li-Jia
authors.initials: LJ
authors.lastname: Qu
authors.forename: Hongya
authors.initials: H
authors.lastname: Gu
authors.forename: Supachitra
authors.initials: S
authors.lastname: Chadchawan
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D001692:Biological Transport; D019295:Computational Biology; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins; D055049:Salt Tolerance; D012492:Salts / Q000494:pharmacology; D036226:Seedlings / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D016415:Sequence Alignment; D013312:Stress, Physiological; D000097796:Nucleolin
chemical_list: D006861:Hydrogen Peroxide; D010750:Phosphoproteins; D010940:Plant Proteins; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D012492:Salts; D000097796:Nucleolin
keywords: Glycine–arginine-rich repeat; Nucleolin; RNA recognition motif; Rice; Salt stress resistance