title: Statistical optimization of alkaline protease production from brackish environment Bacillus sp. SKK11 by SSF using horse gram husk.
abstract: Protease production by Bacillus sp. SKK11 isolated from brackish environment was studied by solid-state fermentation with horse gram husk. Response surface methodology-based Box-Behnken design (BBD) was used to optimize the variables such as pH, maltose, and MgSO₄. The BBD design analysis showed a reasonable adjustment of the quadratic model with the experimental data. Statistics-based contour and three-dimensional (3-D) plots were generated to evaluate the changes in the response surface and to understand the relationship between the enzyme yield and the culture conditions. The maximum yield of the enzyme was observed at pH 9.0.
authors.affiliation: a Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University , Iksan , South Korea.
authors.forename: Muthusamy
authors.initials: M
authors.lastname: Govarthanan
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Park
authors.forename: Jin-Won
authors.initials: JW
authors.lastname: Kim
authors.forename: Kui-Jae
authors.initials: KJ
authors.lastname: Lee
authors.forename: Min
authors.initials: M
authors.lastname: Cho
authors.forename: Seralathan
authors.initials: S
authors.lastname: Kamala-Kannan
authors.forename: Byung-Taek
authors.initials: BT
authors.lastname: Oh
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis*; D003470:Culture Media; D004275:DNA, Ribosomal / Q000235:genetics; D010450:Endopeptidases / Q000096:biosynthesis*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D010450:Endopeptidases; C087822:alkaline protease