title: Determination of okadaic acid in shellfish by using a novel chemiluminescent enzyme-linked immunosorbent assay method.
abstract: A direct competitive chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) was developed to determine okadaic acid (OA). Concentrations of the capture monoclonal anti-OA antibodies, conjugate of OA-HRP and a composition of blocking buffers were varied to optimize the assay condition. The values of IC10, IC50 and working range (IC20-IC80) for CL-ELISA were 0.01, 0.07, and 0.03-0.2 ng/mL, respectively. Additionally, the analytical recovery values of CL-ELISA from 3 shellfish spiked samples with OA concentrations of 0.03, 0.1 and 0.2 ng/mL ranged from 86.7% to 111.2%. Closely examining the OA concentrations in 19 various shellfish products performed by CL-ELISA revealed that OA concentrations in 6 of the 19 examined samples was undetected, whereas the 13 samples were contaminated with low levels of OA ranging from 1.2 to 8.0 ng/g.
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia.
authors.forename: Marina M
authors.initials: MM
authors.lastname: Vdovenko
authors.forename: Chun-Tse
authors.initials: CT
authors.lastname: Hung
authors.forename: Ivan Yu
authors.initials: IY
authors.lastname: Sakharov
authors.forename: Feng-Yih
authors.initials: FY
authors.lastname: Yu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods*; D006735:Horseradish Peroxidase / Q000737:chemistry; D006825:Hybridomas / Q000737:chemistry; D018796:Immunoconjugates / Q000737:chemistry; D008387:Marine Toxins / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008974:Mollusca / Q000737:chemistry*; D019319:Okadaic Acid / Q000032:analysis*; D012758:Shellfish / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D018796:Immunoconjugates; D008387:Marine Toxins; D019319:Okadaic Acid; D006735:Horseradish Peroxidase
keywords: 3-(10′-phenothiazinyl) propane-1-sulfonate; 4-morpholinopyridine; BSA; Bovine serum albumin; CL; Chemiluminescence; ECR; ELISA; Enhanced chemuliminescence reaction; Enhancement; Enzyme-linked immunosorbent assay; HRP; Horseradish peroxidase; MORPH; OA; Okadaic acid; SPTZ; Shellfish