title: Skeletal muscle regeneration on protein-grafted and microchannel-patterned scaffold for hypopharyngeal tissue engineering.
abstract: In the field of tissue engineering, polymeric materials with high biocompatibility like polylactic acid and polyglycolic acid have been widely used for fabricating living constructs. For hypopharynx tissue engineering, skeletal muscle is one important functional part of the whole organ, which assembles the unidirectionally aligned myotubes. In this study, a polyurethane (PU) scaffold with microchannel patterns was used to provide aligning guidance for the seeded human myoblasts. Due to the low hydrophilicity of PU, the scaffold was grafted with silk fibroin (PU-SF) or gelatin (PU-Gel) to improve its cell adhesion properties. Scaffolds were observed to degrade slowly over time, and their mechanical properties and hydrophilicities were improved through the surface grafting. Also, the myoblasts seeded on PU-SF had the higher proliferative rate and better differentiation compared with those on the control or PU-Gel. Our results demonstrate that polyurethane scaffolds seeded with myoblasts hold promise to guide hypopharynx muscle regeneration.
authors.affiliation: Department of Otorhinolaryngology of Lihuili Hospital, Ningbo University, Ningbo 315211, China.
authors.forename: Zhisen
authors.initials: Z
authors.lastname: Shen
authors.forename: Shanshan
authors.initials: S
authors.lastname: Guo
authors.forename: Dong
authors.initials: D
authors.lastname: Ye
authors.forename: Jingjing
authors.initials: J
authors.lastname: Chen
authors.forename: Cheng
authors.initials: C
authors.lastname: Kang
authors.forename: Shejie
authors.initials: S
authors.lastname: Qiu
authors.forename: Dakai
authors.initials: D
authors.lastname: Lu
authors.forename: Qun
authors.initials: Q
authors.lastname: Li
authors.forename: Kunjie
authors.initials: K
authors.lastname: Xu
authors.forename: Jingjing
authors.initials: J
authors.lastname: Lv
authors.forename: Yabin
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D005349:Fibroins / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007013:Hypopharynx / Q000502:physiology*; D055616:Microtechnology / Q000379:methods*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D018482:Muscle, Skeletal / Q000187:drug effects / Q000502:physiology*; D017570:MyoD Protein / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D011140:Polyurethanes / Q000494:pharmacology; D012038:Regeneration / Q000187:drug effects*; D013314:Stress, Mechanical; D013499:Surface Properties; D013718:Tensile Strength / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D017570:MyoD Protein; D011140:Polyurethanes; D014867:Water; D005349:Fibroins