title: Effects of food deprivation on the hypothalamic feeding-regulating peptides gene expressions in serotonin depleted rats.
abstract: We examined the effects of serotonin (5-HT) depletion induced by peripheral injection of 5-HT synthesis inhibitor p-chlorophenylalanine (PCPA) on the expression of feeding-regulating peptides expressions by using in situ hybridization histochemistry in adult male Wistar rats. PCPA pretreatment had no significant effect on basal levels of oxytocin, corticotropin-releasing hormone (CRH), thyrotropin-releasing hormone (TRH), pro-opiomelanocortin (POMC), cocaine and amphetamine-regulated transcript (CART), neuropeptide-Y (NPY), agouti-related protein (AgRP), melanin-concentrating hormone (MCH) or orexin in the hypothalamus. Food deprivation for 48 h caused a significant decrease in CRH, TRH, POMC, and CART, and a significant increase in NPY, AgRP and MCH. After PCPA treatment, POMC and CART did not decrease despite food deprivation. NPY was significantly increased by food deprivation with PCPA, but was attenuated compared to food deprivation without PCPA. These results suggest that the serotonergic system in the hypothalamus may be involved in the gene expression of POMC, CART, and NPY related to feeding behavior.
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Yoshimura
authors.forename: Marina
authors.initials: M
authors.lastname: Hagimoto
authors.forename: Takanori
authors.initials: T
authors.lastname: Matsuura
authors.forename: Junichi
authors.initials: J
authors.lastname: Ohkubo
authors.forename: Motoko
authors.initials: M
authors.lastname: Ohno
authors.forename: Takashi
authors.initials: T
authors.lastname: Maruyama
authors.forename: Toru
authors.initials: T
authors.lastname: Ishikura
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kakuma
authors.forename: Hironobu
authors.initials: H
authors.lastname: Yoshimatsu
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Terawaki
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Uezono
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Toyohira
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Yanagihara
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Ueta
mesh_terms: D000818:Animals; D001835:Body Weight; D004435:Eating; D004791:Enzyme Inhibitors / Q000008:administration & dosage; D005247:Feeding Behavior; D010134:Fenclonine / Q000008:administration & dosage; D005508:Food Deprivation; D005786:Gene Expression Regulation; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D007267:Injections; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism; D036361:Peptide Hormones / Q000235:genetics / Q000378:metabolism*; D011333:Pro-Opiomelanocortin / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000172:deficiency*; D013997:Time Factors; D014365:Tryptophan Hydroxylase / Q000037:antagonists & inhibitors / Q000378:metabolism; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
chemical_list: D004791:Enzyme Inhibitors; D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D036361:Peptide Hormones; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein; D012701:Serotonin; D011333:Pro-Opiomelanocortin; D014365:Tryptophan Hydroxylase; D010134:Fenclonine