title: A cell-based potentiometric biosensor using the fungus Lentinus sajor-caju for permethrin determination in treated wood.
abstract: The characteristics of a potentiometric biosensor for the determination of permethrin in treated wood based on immobilised cells of the fungus Lentinus sajor-caju on a potentiometric transducer are reported this paper. The potentiometric biosensor was prepared by immobilisation of the fungus in alginate gel deposited on a pH-sensitive transducer employing a photocurable acrylic matrix. The biosensor gave a good response in detecting permethrin over the range of 1.0-100.0 µM. The slope of the calibration curve was 56.10 mV/decade with detection limit of 1.00 µM. The relative standard deviation for the sensor reproducibility was 4.86%. The response time of the sensor was 5 min at optimum pH 8.0 with 1.00 mg/electrode of fungus L. sajor-caju. The permethrin biosensor performance was compared with the conventional method for permethrin analysis using high performance liquid chromatography (HPLC), and the analytical results agreed well with the HPLC method (at 95% confidence limit). There was no interference from commonly used organophosphorus pesticides such as diazinon, parathion, paraoxon, and methyl parathion.
authors.affiliation: Forest Products Division, Forest Research Institute Malaysia, Selangor DE 52109, Malaysia.
authors.forename: Mohamad Nasir Mat
authors.initials: MN
authors.lastname: Arip
authors.forename: Lee Yook
authors.initials: LY
authors.lastname: Heng
authors.forename: Musa
authors.initials: M
authors.lastname: Ahmad
authors.forename: Salmiah
authors.initials: S
authors.lastname: Ujang
mesh_terms: D000179:Acrylates / Q000737:chemistry; D000464:Alginates / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D002138:Calibration; D018914:Cells, Immobilized; D004566:Electrodes; D005782:Gels; D006863:Hydrogen-Ion Concentration; D007306:Insecticides / Q000032:analysis*; D020074:Lentinula / Q000737:chemistry*; D057230:Limit of Detection; D026023:Permethrin / Q000032:analysis*; D011199:Potentiometry / Q000379:methods; D015203:Reproducibility of Results; D014934:Wood / Q000737:chemistry*
chemical_list: D000179:Acrylates; D000464:Alginates; D005782:Gels; D007306:Insecticides; D026023:Permethrin
keywords: Alginate; Lentinus sajor-caju; Permethrin; Wood preservative analysis