title: Prefusion structure of trimeric HIV-1 envelope glycoprotein determined by cryo-electron microscopy.
abstract: The activation of trimeric HIV-1 envelope glycoprotein (Env) by its binding to the cell-surface receptor CD4 and co-receptors (CCR5 or CXCR4) represents the first of a series of events that lead to fusion between viral and target-cell membranes. Here, we present the cryo-EM structure, at subnanometer resolution (~6 Å at 0.143 FSC), of the 'closed', prefusion state of trimeric HIV-1 Env complexed to the broadly neutralizing antibody VRC03. We show that three gp41 helices at the core of the trimer serve as an anchor around which the rest of Env is reorganized upon activation to the 'open' quaternary conformation. The architecture of trimeric HIV-1 Env in the prefusion state and in the activated intermediate state resembles the corresponding states of influenza hemagglutinin trimers, thus providing direct evidence for the similarity in entry mechanisms used by HIV-1, influenza and related enveloped viruses.
authors.affiliation: Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Alberto
authors.initials: A
authors.lastname: Bartesaghi
authors.forename: Alan
authors.initials: A
authors.lastname: Merk
authors.forename: Mario J
authors.initials: MJ
authors.lastname: Borgnia
authors.forename: Jacqueline L S
authors.initials: JL
authors.lastname: Milne
authors.forename: Sriram
authors.initials: S
authors.lastname: Subramaniam
mesh_terms: D057134:Antibodies, Neutralizing / Q000737:chemistry; D020285:Cryoelectron Microscopy; D015700:HIV Envelope Protein gp41 / Q000737:chemistry*; D015497:HIV-1; D008958:Models, Molecular; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D013329:Structure-Activity Relationship; D053586:Virus Internalization
chemical_list: D057134:Antibodies, Neutralizing; D015700:HIV Envelope Protein gp41