title: Study on the effect of polysaccharides from Solanum nigrum Linne on cellular immune function in tumour-bearing mice.
abstract: We investigated the anti-tumour effect of polysaccharides from Solanum nigrum Linne, and its relationship with the immune function of tumour-bearing organisms. MTT assay was used to observe the effect of different doses of polysaccharides from Solanum nigrum Linne on proliferation of lymphocytes in tumour-bearing mice. ELISA assay was also used to detect the levels of IL-2 in mice, and a laser scanning confocal microscope was used to detect the effect of polysaccharides from Solanum nigrum Linne on intralymphocytic free calcium ion concentration in tumour-bearing mice. Different doses of polysaccharides from Solanum nigrum Linne significantly inhibited the growth of mouse H22 solid tumours, improved the survival time of tumour-bearing mice, increased the proliferation of lymphocytes, elevated the levels of IL-2, and increased the concentration of calcium ions in the lymphocytes. Polysaccharides from Solanum nigrum Linne have certain anti-tumour effect, which is related with the cellular immune function that regulates the body.
authors.affiliation: Department of Galactophore, The Military General Hospital of Beijing PLA, Beijing, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Chen
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Qi
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use*; D002118:Calcium / Q000378:metabolism; D007376:Interleukin-2 / Q000097:blood; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D009369:Neoplasms / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D011134:Polysaccharides / Q000494:pharmacology / Q000627:therapeutic use*; D032324:Solanum nigrum / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D007376:Interleukin-2; D010936:Plant Extracts; D011134:Polysaccharides; D002118:Calcium
keywords: Solanum nigrum polysaccharides; cellular immunity; tumour-bearing mice