title: Comprehensive two-dimensional gas chromatography combined to multivariate data analysis for detection of disease-resistant clones of Eucalyptus.
abstract: In this paper it is reported the use of the chromatographic profiles from volatile fractions of plant clones - in this case, hybrids of Eucalyptus grandis×Eucalyptus urophylla - to determine specimens susceptible to rust disease. The analytes were isolated by headspace solid phase microextraction (HS-SPME) and analyzed by comprehensive two-dimensional gas chromatography combined to fast quadrupole mass spectrometry (GC×GC-qMS). Parallel Factor Analysis (PARAFAC) was employed for estimate the correlation between the chromatographic profiles and resistance against Eucalyptus rust, after preliminary variable selection performed by Fisher ratio analysis. The proposed method allowed the differentiation between susceptible and non-susceptible clones and determination of three resistance biomarkers. This approach can be a valuable alternative for the otherwise time-consuming and labor-intensive methods commonly used.
authors.affiliation: Institute of Chemistry, University of Campinas (Unicamp) and Instituto Nacional de Ciência e Tecnologia em Bioanalítica (INCTBio), IQ, Unicamp, CP 6154, 13084-971 Campinas, São Paulo, Brazil.
authors.forename: Leandro Wang
authors.initials: LW
authors.lastname: Hantao
authors.forename: Bruna Regina
authors.initials: BR
authors.lastname: Toledo
authors.forename: Fabiana Alves de Lima
authors.initials: FA
authors.lastname: Ribeiro
authors.forename: Marilia
authors.initials: M
authors.lastname: Pizetta
authors.forename: Caroline Geraldi
authors.initials: CG
authors.lastname: Pierozzi
authors.forename: Edson Luiz
authors.initials: EL
authors.lastname: Furtado
authors.forename: Fabio
authors.initials: F
authors.lastname: Augusto
mesh_terms: D015415:Biomarkers / Q000032:analysis; D002678:Chimera; D005052:Eucalyptus / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D015999:Multivariate Analysis; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D057865:Plant Immunity; D018515:Plant Leaves / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D052617:Solid Phase Microextraction; D055549:Volatile Organic Compounds / Q000032:analysis*
chemical_list: D015415:Biomarkers; D055549:Volatile Organic Compounds
keywords: Comprehensive two-dimensional gas chromatography; Mass spectrometry; Metabolic profile; Parallel factor analysis; Puccinia psidii; Resistance biomarkers