title: Polysaccharides from prunes: gastroprotective activity and structural elucidation of bioactive pectins.
abstract: Prunes are the dried fruits from Prunus domestica. After the purification steps, two homogeneous polysaccharides were characterised, SF-50R and SF-50E and contained Ara:Gal:Rha:GalA in 47.8:31.5:10.7:10.0 and 39.6:50.3:5.1:5.0 molar ratios, respectively. Methylation analysis and (13)C NMR spectroscopy indicated that both fractions are constituted by rhamnogalacturonans with type I arabinogalactans as side chains, differing mainly in the proportions of the rhamnogalacturonan backbone, in the length of the (1→4)-β-galactan chain and in the proportion of the arabinan side chain. Crude water extract (PWH) and fraction SF-50E were evaluated for their gastroprotective properties against ethanol-induced acute gastric lesions in rats. Oral administration of PWH (3 and 10mg/kg) reduced the gastric lesion area by 67±11% and 60±12%, respectively, while fraction SF-50E (10 and 30mg/kg) inhibited the lesion area by 84±12% and 83±12%, respectively. These results indicated that prune's polysaccharides act as gastroprotective agents in rats.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980 Curitiba, PR, Brazil.
authors.forename: Thaisa Moro
authors.initials: TM
authors.lastname: Cantu-Jungles
authors.forename: Daniele
authors.initials: D
authors.lastname: Maria-Ferreira
authors.forename: Luisa Mota
authors.initials: LM
authors.lastname: da Silva
authors.forename: Cristiane Hatsuko
authors.initials: CH
authors.lastname: Baggio
authors.forename: Maria Fernanda de Paula
authors.initials: MF
authors.lastname: Werner
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacomini
authors.forename: Thales R
authors.initials: TR
authors.lastname: Cipriani
authors.forename: Lucimara M C
authors.initials: LM
authors.lastname: Cordeiro
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D005260:Female; D005756:Gastritis / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D008969:Molecular Sequence Data; D010368:Pectins / Q000008:administration & dosage* / Q000737:chemistry; D020011:Protective Agents / Q000008:administration & dosage* / Q000737:chemistry; D027861:Prunus / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D020011:Protective Agents; D010368:Pectins
keywords: Gastroprotective activity; Prunes; Prunus domestica; Rhamnogalacturonan; Type I arabinogalactan