title: Local assessment of myelin health in a multiple sclerosis mouse model using a 2D Fourier transform approach.
abstract: We present an automated two-dimensional Fourier transform (2D-FT) approach to analyze the local organization of myelinated axons in the spinal cord. Coherent anti-Stokes Raman scattering (CARS) microscopy was used to observe lesions in a commonly used animal model of multiple sclerosis (MS), experimental autoimmune encephalomyelitis (EAE). A 2D-FT was applied on the CARS images to find the average orientation and directional anisotropy of the fibers within contiguous image domains. We introduce the corrected correlation parameter (CCP), a measure of the correlation between orientations of adjacent domains. We show that in the EAE animal model of MS, the CCP can be used to quantify the degree of organization/disorganization in the myelin structure. This analysis was applied to a large image dataset from animals at different clinical scores and we show that some descriptors of the CCP probability density function are strongly correlated with the clinical scores. This procedure, compatible with live animal imaging, has been developed to perform local in situ evaluation of myelinated axons afflicted by EAE.
authors.affiliation: Centre de recherche de l'Institut universitaire en santé mentale de Québec (CRIUSMQ), Université Laval, Québec, Canada ; Département de physique, génie physique et optique, Université Laval, Québec, Canada ; Centre d'optique, photonique et laser (COPL), Université Laval, Québec, Canada.
authors.forename: Steve
authors.initials: S
authors.lastname: Bégin
authors.forename: Erik
authors.initials: E
authors.lastname: Bélanger
authors.forename: Sophie
authors.initials: S
authors.lastname: Laffray
authors.forename: Benoît
authors.initials: B
authors.lastname: Aubé
authors.forename: Emilie
authors.initials: E
authors.lastname: Chamma
authors.forename: Jonathan
authors.initials: J
authors.lastname: Bélisle
authors.forename: Steve
authors.initials: S
authors.lastname: Lacroix
authors.forename: Yves
authors.initials: Y
authors.lastname: De Koninck
authors.forename: Daniel
authors.initials: D
authors.lastname: Côté
keywords: (000.1430) Biology and medicine; (100.2960) Image analysis; (170.3880) Medical and biological imaging; (170.4580) Optical diagnostics for medicine; (180.4315) Nonlinear microscopy; (180.6900) Three-dimensional microscopy; (300.6230) Spectroscopy, coherent anti-Stokes Raman scattering