title: Fast and accurate method to purify small noncoding RNAs from Drosophila ovaries.
abstract: The recent development of High Throughput Sequencing technology has boosted the study of small regulatory RNA populations. A critical step prior to cloning and sequencing is purification of small RNA populations. Here, we report the optimization of an anion-exchange chromatography procedure in order to purify small regulatory RNAs bound on proteins. We developed this procedure to make it less time-consuming since our improved method no longer requires specific equipment and can easily be performed at the bench. We believe that our procedure will increase the robustness and accuracy of small RNA libraries in the future.
authors.affiliation: Institut de Génétique Humaine, CNRS, Montpellier, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Grentzinger
authors.forename: Séverine
authors.initials: S
authors.lastname: Chambeyron
mesh_terms: D000818:Animals; D001483:Base Sequence; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004210:Dissection; D004331:Drosophila melanogaster; D005260:Female; D020868:Gene Silencing; D010053:Ovary / Q000378:metabolism* / Q000601:surgery; D034741:RNA, Small Interfering / Q000235:genetics / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D034741:RNA, Small Interfering