title: Measuring of Mycobacterium tuberculosis growth. A correlation of the optical measurements with colony forming units.
abstract: The quantification of colony forming units (cfu), turbidity, and optical density at 600 nm (OD600) measurements were used to evaluate Mycobacterium tuberculosis growth. Turbidity and OD600 measurements displayed similar growth curves, while cfu quantification showed a continuous growth curve. We determined the cfu equivalents to McFarland and OD600 units.
authors.affiliation: Laboratorio de Biología Molecular, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Monterrey, NL, México. ; Posgrado en Microbiología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, NL, México.
authors.forename: Katia
authors.initials: K
authors.lastname: Peñuelas-Urquides
authors.forename: Licet
authors.initials: L
authors.lastname: Villarreal-Treviño
authors.forename: Beatriz
authors.initials: B
authors.lastname: Silva-Ramírez
authors.forename: Liliana
authors.initials: L
authors.lastname: Rivadeneyra-Espinoza
authors.forename: Salvador
authors.initials: S
authors.lastname: Said-Fernández
authors.forename: Mario Bermúdez
authors.initials: MB
authors.lastname: de León
keywords: McFarland; Mycobacterium tuberculosis; bacterial growth; colony forming units; optical density