title: Monoclonal antibody-quantum dots CdTe conjugate-based fluoroimmunoassay for the determination of aflatoxin B1 in peanuts.
abstract: A fluoroimmunoassay towards aflatoxin B1 (AFB1) was presented using quantum dots as the fluorescent label. The CdTe QDs were successfully linked to the monoclonal antibody against AFB1. Based on the conjugated complexes, a novel direct competitive fluorescence-linked immunosorbent assay (cFLISA) was developed for AFB1 detection. The 50% inhibition value (IC50) of the cFLISA was 0.149ng/mL in peanuts matrix. The method performance included the limit of detection (LOD) of 0.016ng/mL and considerable recoveries of 85-117% at three fortification levels (0.075, 0.15, and 0.3ng/g) from spiked AFB1 blank peanuts samples, along with coefficients of variation (CVs) below 10%. The cFLISA provided an alternative of rapid and sensitive detection for AFB1 and, moreover provided great potential for multiplexed mycotoxins determination simultaneously.
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, PR China; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, PR China.
authors.forename: Zhaowei
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.forename: Peiwu
authors.initials: P
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Hu
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Ding
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000032:analysis; D010367:Arachis / Q000737:chemistry*; D015200:Fluoroimmunoassay / Q000295:instrumentation / Q000379:methods*; D045663:Quantum Dots / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D016604:Aflatoxin B1
keywords: Aflatoxin B(1); Fluoroimmunoassay; Peanuts; Quantum dots CdTe