title: Characterization of multidrug-resistant Klebsiella pneumoniae from Australia carrying blaNDM-1.
abstract: blaNDM genes, encoding metallo-β-lactamases providing resistance to carbapenems, have been reported in many locations since the initial report in 2008, including in several Enterobacteriaceae isolates in Australia/New Zealand. Here, we compare 4 additional carbapenem-resistant Klebsiella pneumoniae carrying blaNDM-1 isolated in Australia. Two are sequence type ST147, previously associated with blaNDM in Australia and elsewhere. They carry blaNDM-1 and different 16S rRNA methylase genes (armA or rmtC) on different conjugative plasmids, in 1 case with an IncFIIY replicon. One isolate belongs to the globally important ST11 but did not transfer a plasmid to Escherichia coli. The fourth isolate belongs to the novel ST1068 and transferred blaNDM-1, armA, and an IncA/C plasmid. Amplification and sequencing of ompK porin genes suggest that, unlike the case for other carbapenemase genes, ompK36 defects may not be required for NDM to cause clinically relevant levels of carbapenem resistance.
authors.affiliation: Centre for Research Excellence in Critical Infection and Marie Bashir Institute for Infectious Diseases and Biosecurity, University of Sydney, Sydney, NSW 2145, Australia; Westmead Millennium Institute, Westmead, NSW 2145, Australia; International Centre for Diarrhoeal Disease Research, Bangladesh, Mohakhali, Dhaka 1212, Bangladesh.
authors.forename: Shereen
authors.initials: S
authors.lastname: Shoma
authors.affiliation: Centre for Research Excellence in Critical Infection and Marie Bashir Institute for Infectious Diseases and Biosecurity, University of Sydney, Sydney, NSW 2145, Australia; Westmead Millennium Institute, Westmead, NSW 2145, Australia; International Centre for Diarrhoeal Disease Research, Bangladesh, Mohakhali, Dhaka 1212, Bangladesh.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Kamruzzaman
authors.affiliation: Centre for Research Excellence in Critical Infection and Marie Bashir Institute for Infectious Diseases and Biosecurity, University of Sydney, Sydney, NSW 2145, Australia; Westmead Millennium Institute, Westmead, NSW 2145, Australia; Centre for Infectious Diseases and Microbiology, Westmead Hospital, Westmeadx, NSW 2145, Australia.
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Ginn
authors.affiliation: Centre for Research Excellence in Critical Infection and Marie Bashir Institute for Infectious Diseases and Biosecurity, University of Sydney, Sydney, NSW 2145, Australia; Westmead Millennium Institute, Westmead, NSW 2145, Australia; Centre for Infectious Diseases and Microbiology, Westmead Hospital, Westmeadx, NSW 2145, Australia.
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Iredell
authors.affiliation: Centre for Research Excellence in Critical Infection and Marie Bashir Institute for Infectious Diseases and Biosecurity, University of Sydney, Sydney, NSW 2145, Australia; Westmead Millennium Institute, Westmead, NSW 2145, Australia; Centre for Infectious Diseases and Microbiology, Westmead Hospital, Westmeadx, NSW 2145, Australia. Electronic address: sally.partridge@swahs.health.nsw.gov.au.
authors.forename: Sally R
authors.initials: SR
authors.lastname: Partridge
mesh_terms: D000818:Animals; D001315:Australia / Q000453:epidemiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D018272:Porins / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D018272:Porins; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1
keywords: Klebsiella pneumoniae; bla(NDM); ompK