title: Overview of thermostable DNA polymerases for classical PCR applications: from molecular and biochemical fundamentals to commercial systems.
abstract: During the genomics era, the use of thermostable DNA polymerases increased greatly. Many were identified and described-mainly of the genera Thermus, Thermococcus and Pyrococcus. Each polymerase has different features, resulting from origin and genetic modification. However, the rational choice of the adequate polymerase depends on the application itself. This review gives an overview of the most commonly used DNA polymerases used for PCR application: KOD, Pab (Isis™), Pfu, Pst (Deep Vent™), Pwo, Taq, Tbr, Tca, Tfi, Tfl, Tfu, Tgo, Tli (Vent™), Tma (UITma™), Tne, Tth and others.
authors.affiliation: SensoQuest GmbH, Hannah-Vogt-Str. 1, 37085, Göttingen, Germany, k.terpe@sensoquest.de.
authors.forename: Kay
authors.initials: K
authors.lastname: Terpe
mesh_terms: D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000378:metabolism*; D004795:Enzyme Stability; D006358:Hot Temperature; D016133:Polymerase Chain Reaction / Q000379:methods*; D055550:Protein Stability
chemical_list: D004259:DNA-Directed DNA Polymerase