title: PET-dependent fluorescence sensing of enzyme reactions using the large and tunable pKa shift of aliphatic amines.
abstract: A fluorescence sensing system exploiting the large and finely tunable pKa shift of aliphatic amines was developed. The amine-containing fluorescent probes with distinct pKa values in a wide pH range were successfully applied to detect a variety of enzyme reactions with a large and real-time fluorescence enhancement.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan. ojida@phar.kyushu-u.ac.jp.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Oshikawa
authors.forename: Akio
authors.initials: A
authors.lastname: Ojida
mesh_terms: D000588:Amines / Q000737:chemistry*; D001896:Boron Compounds / Q000737:chemistry / Q000378:metabolism; D004579:Electron Transport; D004950:Esterases / Q000032:analysis* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008247:Lysosomes / Q000378:metabolism; D018613:Microscopy, Confocal
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D000588:Amines; D001896:Boron Compounds; D005456:Fluorescent Dyes; D004950:Esterases