title: Transgenic plant production from leaf discs of Moricandia arvensis using Agrobacterium tumefaciens.
abstract: A high frequency shoot regeneration (80%) was developed from callus of leaf discs and stem internodes of Moricandia arvensis. Leaf discs were shown to be a preferable starting material for transformation experiments. Agrobacterium tumefaciens strain GV3101/pMP90 used in this study contained a binary vector with genes for kanamycin resistance, hygromycin resistance and β-glucuronidase (GUS). Maximum transformation efficiency (10.3%) was achieved by using kanamycin at the rate of 200 mg/l as a selection agent. Presence of tobacco suspension culture during co-cultivation and a pre-selection period of seven days after co-cultivation was essential for successful transformation. Transgenic plants grew to maturity and exhibited flowering in a glasshouse. GUS activity was evident in all parts of leaf and the presence of GUS gene in plant gemone was confirmed by PCR analysis.
authors.affiliation: Laboratory of Plant Breeding, Faculty of Agriculture, Tohoku University, 981, Sendai, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Rashid
authors.forename: K
authors.initials: K
authors.lastname: Toriyama
authors.forename: K
authors.initials: K
authors.lastname: Hinata