title: MicroRNA-210 overexpression induces angiogenesis and neurogenesis in the normal adult mouse brain.
abstract: Angiogenesis and neurogenesis are crucial processes for brain tissue repair and remodeling after brain injury. Current study shows that microRNA-210 (miR-210) promotes vascular endothelial cell migration and tube formation under hypoxia in vitro. Whether miR-210 overexpression promotes focal angiogenesis and neurogenesis in the normal adult brain is unknown. Adult male C57BL/6 mice (n=54) underwent stereotactic injection of a lentiviral vector carrying miR-210 (LV-miR-210). Following 28 days of miR-210 gene transfer, endothelial cell and neural precursor cell proliferation, microvessel density and downstream angiogenic factor were genotyped. miR-210 was highly expressed in neurons, astrocytes and endothelial cells of the LV-miR-210-injected brain hemisphere. The endothelial cell proliferation and the number of newly formed microvessels were greatly increased in the LV-miR-210-treated mice compared with the controls (P<0.05). Neural progenitor cells in the subventricular zone were greatly increased compared with the controls (P<0.05). The data indicate that miR-210 is a key factor at the microRNA level in promoting angiogenesis and neurogenesis, which was associated with local increased vascular endothelial growth factor (VEGF) levels, suggesting that miR-210 may be a potential target for ischemic stroke therapy.
authors.affiliation: 1] Department of Neurology, Ruijin Hospital, Shanghai, China [2] Neuroscience and Neuroengineering Center, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: L
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Neuroscience and Neuroengineering Center, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: X
authors.initials: X
authors.lastname: He
authors.affiliation: 1] Department of Neurology, Ruijin Hospital, Shanghai, China [2] Neuroscience and Neuroengineering Center, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Neuroscience and Neuroengineering Center, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Neurology, Ruijin Hospital, Shanghai, China.
authors.forename: C
authors.initials: C
authors.lastname: Zheng
authors.affiliation: 1] Department of Neurology, Ruijin Hospital, Shanghai, China [2] Neuroscience and Neuroengineering Center, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: H
authors.initials: H
authors.lastname: Cai
authors.affiliation: Department of Neurology, Ruijin Hospital, Shanghai, China.
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.affiliation: 1] Neuroscience and Neuroengineering Center, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China [2] School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Neurology, Ruijin Hospital, Shanghai, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Fu
authors.affiliation: 1] Department of Neurology, Ruijin Hospital, Shanghai, China [2] Neuroscience and Neuroengineering Center, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China [3] School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: G-Y
authors.initials: GY
authors.lastname: Yang
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001921:Brain / Q000098:blood supply / Q000502:physiology*; D042783:Endothelial Cells / Q000378:metabolism; D005822:Genetic Vectors; D016086:Lentivirus / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D055806:Microvessels / Q000378:metabolism; D018919:Neovascularization, Physiologic; D055495:Neurogenesis; D009474:Neurons / Q000378:metabolism; D014161:Transduction, Genetic; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: C549325:MIRN210 microRNA, mouse; D035683:MicroRNAs; D042461:Vascular Endothelial Growth Factor A