title: Effect of oxygen-enriched aeration on regeneration of rice (Oryza sativa L.) cell culture.
abstract: The effect of the oxygen concentration in the aeration gas on regeneration from rice cells in bioreactor cultures was investigated. The efficiency of regeneration in cultures aerated with over 40% oxygen was higher than that in a flask culture. In the case of a culture in which the dissolved oxygen(DO) was saturated by aeration with air, the efficiency of regeneration was less than the half that of cultures aerated with 40% oxygen. In cultures with the DO levels controlled at 8,10 and 12 mg/ℓ, the efficiency of regeneration was highest at 12 mg/ℓ. In the oxygenenriched cultures, although cell aggregation was observed and the color of plantlets was relatively pale, more than 90% of them grew into healthy plants.
authors.affiliation: First Research Center, Nursery Technology Inc., Kitsuregawa-machi, Shioya-gun, 329-14, Tochigi, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Okamoto
authors.forename: S
authors.initials: S
authors.lastname: Kishine
authors.forename: T
authors.initials: T
authors.lastname: Hirosawa
authors.forename: A
authors.initials: A
authors.lastname: Nakazono