title: Construction of isogenic mutants in Streptococcus gallolyticus based on the development of new mobilizable vectors.
abstract: Streptococcus gallolyticus is an emerging cause of infective endocarditis that has been epidemiologically linked to colorectal cancer. S. gallolyticus is poorly transformable using electroporation and no defined mutant has been published yet. Hence, we used mobilization to introduce plasmid DNA from Streptococcus agalactiae into S. gallolyticus using the transfer origin of the conjugative element TnGBS1 (oriTTnGBS1), followed by a classical homologous recombination technique. Two isogenic mutants of S. gallolyticus UCN34, one deleted for the pil1 pilus operon and another for the sortase A gene, were constructed and characterized. This genetic tool should help in unravelling virulence mechanisms of this bacterium.
authors.affiliation: Institut Pasteur, Unité de Biologie des Bactéries Pathogènes à Gram positif, Paris F-75015, France; CNRS, ERL 3526, France; Université Paris Diderot-Sorbonne Paris Cité, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Danne
authors.forename: Romain
authors.initials: R
authors.lastname: Guérillot
authors.forename: Philippe
authors.initials: P
authors.lastname: Glaser
authors.forename: Patrick
authors.initials: P
authors.lastname: Trieu-Cuot
authors.forename: Shaynoor
authors.initials: S
authors.lastname: Dramsi
mesh_terms: D003227:Conjugation, Genetic; D017353:Gene Deletion; D005822:Genetic Vectors; D005827:Genetics, Microbial / Q000379:methods*; D059765:Homologous Recombination; D008967:Molecular Biology / Q000379:methods*; D010957:Plasmids; D013291:Streptococcus / Q000235:genetics*; D014169:Transformation, Bacterial
keywords: Conjugation; Isogenic mutant; Mobilizable plasmids; Streptococcus bovis; Streptococcus gallolyticus; oriTTnGBS1