title: Genetic characterization of a betanodavirus isolated from a clinical disease outbreak in farm-raised tilapia Oreochromis niloticus (L.) in Thailand.
abstract: Betanodavirus infection was diagnosed in larvae of farm-raised tilapia Oreochromis niloticus (L.), in central Thailand. Extensive vacuolar degeneration and neuronal necrosis were observed in histological sections with positive immunohistochemical staining for betanodavirus. Molecular phylogenetic analysis was performed based on the nucleotide sequences (1333 bases) of the capsid protein gene. The virus strain was highly homologous (93.07-93.88%) and closely related to red-spotted grouper nervous necrosis virus (RGNNV).
authors.affiliation: Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
authors.forename: J
authors.initials: J
authors.lastname: Keawcharoen
authors.forename: S
authors.initials: S
authors.lastname: Techangamsuwan
authors.forename: A
authors.initials: A
authors.lastname: Ponpornpisit
authors.forename: E D
authors.initials: ED
authors.lastname: Lombardini
authors.forename: T
authors.initials: T
authors.lastname: Patchimasiri
authors.forename: N
authors.initials: N
authors.lastname: Pirarat
mesh_terms: D000818:Animals; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D023681:Cichlids / Q000821:virology; D005393:Fish Diseases / Q000473:pathology / Q000821:virology*; D008969:Molecular Sequence Data; D030381:Nodaviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012327:RNA Virus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D012689:Sequence Homology, Nucleic Acid; D013785:Thailand
chemical_list: D036022:Capsid Proteins
keywords: Genetic characteristic; Thailand; betanodavirus; farm-raised tilapia