title: The effects of β-elemene on the expression of mTOR, HIF-1A, survivin in lung adenocarcinoma A549 cell.
abstract: The purpose of this manuscript was to study the regulation effects of β-elemene combined with radiotherapy on three different gene expressions in lung adenocarcinoma A549 cell. mTOR gene, HIF-1α gene, Survivin gene were included in the gene group. Cell culture and RT-PCR were applied to finish this research. Hypoxia Control group, Hypoxia β-elemene group, Hypoxia β-elemene combined with irradiation group were set to compare the differences of three different gene expressions. The most active effects were found in the group of Hypoxia irradiation combined with β-elemene. In this group, the mTOR gene, HIF-1α gene, Survivin gene expressions were all down-regulated when compared with the single treatment groups, and there were significantly statistical differences.
authors.affiliation: Department of Radiation Oncology, Dalian Medical University.
authors.forename: Enjuan
authors.initials: E
authors.lastname: Tong
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Xu
authors.forename: Guoquan
authors.initials: G
authors.lastname: Li
authors.forename: Kun
authors.initials: K
authors.lastname: Zou
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zou
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy / Q000378:metabolism* / Q000532:radiotherapy; D000077192:Adenocarcinoma of Lung; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use; D045744:Cell Line, Tumor; D017024:Chemotherapy, Adjuvant; D030024:Curcuma / Q000737:chemistry*; D015536:Down-Regulation; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D000860:Hypoxia; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism*; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000532:radiotherapy; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012717:Sesquiterpenes / Q000494:pharmacology* / Q000627:therapeutic use; D000077022:Survivin; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C107489:BIRC5 protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D051034:Inhibitor of Apoptosis Proteins; D010936:Plant Extracts; D012717:Sesquiterpenes; D000077022:Survivin; C445979:beta-elemene; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases
keywords: A549; HIF-1α; Rhizoma Curcumae; Survivin; mTOR; β-elemene