title: [3-(Trifluoromethyl)-3H-diazirin-3-yl]coumarin as a carbene-generating photocross-linker with masked fluorogenic beacon.
abstract: A coumarin-fused diazirine photolabeling agent exhibited dramatic enhancement in fluorescence after cross-linking with the target protein. Fluorescence emission from the coumarin moiety was efficiently quenched by the diazirine group, and was then intensively recovered from photolysis treatment by irradiation with light at a wavelength of 365 nm.
authors.affiliation: Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan. ttomo@pha.u-toyama.ac.jp yasu@pha.u-toyama.ac.jp.
authors.forename: Takenori
authors.initials: T
authors.lastname: Tomohiro
authors.forename: Akito
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Yoko
authors.initials: Y
authors.lastname: Tatsumi
authors.forename: Yasumaru
authors.initials: Y
authors.lastname: Hatanaka
mesh_terms: D016227:Benzoquinones / Q000737:chemistry; D003374:Coumarins / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D003978:Diazomethane / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D018841:HSP90 Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry*; D047029:Lactams, Macrocyclic / Q000737:chemistry; D008697:Methane / Q000031:analogs & derivatives / Q000737:chemistry; D010782:Photolysis
chemical_list: D016227:Benzoquinones; D003374:Coumarins; D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D018841:HSP90 Heat-Shock Proteins; D047029:Lactams, Macrocyclic; C030011:carbene; D003978:Diazomethane; D008697:Methane; C001277:geldanamycin