title: Minimum set of cytochromes P450 for reconstituting the biosynthesis of camalexin, a major Arabidopsis antibiotic.
abstract: Bringing it all together: The missing key step in the biosynthesis of camalexin was uncovered by in vitro biochemical characterization. The coupling of Trp- and Cys-derived fragments through CS bond formation is promoted by an unusual cytochrome P450 CYP71A13. The in vitro reconstitution of the camalexin biosynthesis (left) from Trp and Cys was achieved using just three cytochromes P450. IAN=indole-3-acetonitrile.
authors.affiliation: Department of Chemical Engineering, Stanford University, Stanford, CA 94305 (USA).
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Klein
authors.forename: Gülbenk
authors.initials: G
authors.lastname: Anarat-Cappillino
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: Sattely
mesh_terms: D017360:Arabidopsis; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001688:Biological Products; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D007211:Indoles / Q000138:chemical synthesis* / Q000737:chemistry; D013844:Thiazoles / Q000138:chemical synthesis* / Q000737:chemistry; D000094162:Phytoalexins
chemical_list: D029681:Arabidopsis Proteins; D001688:Biological Products; D003577:Cytochrome P-450 Enzyme System; D007211:Indoles; D013844:Thiazoles; C102405:camalexin; D000094162:Phytoalexins
keywords: CS bond formation; biosynthesis; camalexin; cytochromes; natural products