title: Homologous constitutive expression of Xyn III in Trichoderma reesei QM9414 and its characterization.
abstract: Xylanase III (Xyn III), a specific endoxylanase that belongs to family 10 of the glycoside hydrolases, was overexpressed in Trichoderma reesei QM9414 using a constitutive strong promoter of the gene encoding pyruvate decarboxylase (pdc). The maximum recombinant xylanase activity achieved was 817.2 ± 65.2 U/mL in the transformant fermentation liquid. The productivities of Xyn III accounted for approximately 53% of the total protein secreted by the recombinant. The enzyme was optimally active at 60 °C and pH 6. The recombinant Xyn III was stable at pH 5-8. This is the first report on the homologous expression of xyn3 in T. reesei QM9414. The properties of Xyn III make it promising in a variety of industrial use.
authors.affiliation: Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen, Guangdong, 518060, People's Republic of China, wangjuan@szu.edu.cn.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Desheng
authors.initials: D
authors.lastname: Zeng
authors.forename: Guoqin
authors.initials: G
authors.lastname: Mai
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Shaowen
authors.initials: S
authors.lastname: Yu
mesh_terms: D003001:Cloning, Molecular; D043364:Endo-1,4-beta Xylanases / Q000235:genetics* / Q000378:metabolism; D004789:Enzyme Activation; D004795:Enzyme Stability; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D014242:Trichoderma / Q000235:genetics* / Q000378:metabolism
chemical_list: D043364:Endo-1,4-beta Xylanases