title: Electromechanical effects of 1,25-dihydroxyvitamin d with antiatrial fibrillation activities.
abstract: BACKGROUND
  Treatment with 1,25-dihydroxyvitamin D (1,25[OH]2 D) has several cardiovascular benefits. 1,25[OH]2 D has direct cellular effects, but its effects on the atrium are not clear. We evaluated the effects of 1,25[OH]2 D on the atrial electrophysiology and atrial fibrillation (AF).
  
  
  METHODS
  Conventional microelectrodes were used to record action potentials (APs) and contractility in isolated rabbit left atrium (LA) tissue preparations before and after the administration of 0.01, 0.1, and 1 nM 1,25[OH]2 D with and without rapid atrial pacing (RAP) and acetylcholine (5 mM)-induced AF. Surface ECG and intracardiac electrograms were recorded before and after the intravenous administration of 4 units/kg of 1,25[OH]2 D in heart failure (HF) rabbits (4 weeks after coronary artery ligation) with RAP and acetylcholine-induced AF.
  
  
  RESULTS
  1,25[OH]2 D dose-dependently increased the AP duration in the LA, which was abolished by pretreatment with 0.1 μM ryanodine. RAP and 5 mM acetylcholine-induced fewer (64.3% vs 100%, P < 0.05) AF occurrences in the presence (n = 14) of 1,25[OH]2 D than those (n = 14) in the absence of 1,25[OH]2 D. The LA treated with 1,25[OH]2 D (n = 9) had a slower maximal AF rate (10.9 ± 2.4 Hz vs 13.3 ± 2.7 Hz, P < 0.05) than the LA (n = 14) without 1,25[OH]2 D. Moreover, 1,25[OH]2 D caused a lower AF inducible percentage (11.0 ± 1.9% vs 100 ± 0%, P < 0.001) and a shorter duration (4 ± 0.4 seconds vs 309 ± 26 seconds, P < 0.001) with a prolonged LA 90% monophasic AP duration (94.1 ± 0.2 milliseconds vs 98.5 ± 0.1 milliseconds, P < 0.05) in 5 rabbits with HF. 1,25[OH]2 D did not prolong the QT interval or 90% of the AP duration in isolated Purkinje fibers.
  
  
  CONCLUSIONS
  1,25[OH]2 D has direct electromechanical effects on the LA and can prevent or terminate AF.
authors.affiliation: Division of Cardiology and Cardiovascular Research Center, Veterans General Hospital-Taipei, Taipei, Taiwan.
authors.forename: Dicky A
authors.initials: DA
authors.lastname: Hanafy
authors.forename: Shih-Lin
authors.initials: SL
authors.lastname: Chang
authors.forename: Yen-Yu
authors.initials: YY
authors.lastname: Lu
authors.forename: Yao-Chang
authors.initials: YC
authors.lastname: Chen
authors.forename: Yu-Hsun
authors.initials: YH
authors.lastname: Kao
authors.forename: Jen-Hung
authors.initials: JH
authors.lastname: Huang
authors.forename: Shih-Ann
authors.initials: SA
authors.lastname: Chen
authors.forename: Yi-Jen
authors.initials: YJ
authors.lastname: Chen
mesh_terms: D000200:Action Potentials / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D001145:Arrhythmias, Cardiac / Q000503:physiopathology; D001281:Atrial Fibrillation / Q000188:drug therapy* / Q000503:physiopathology; D001696:Biomechanical Phenomena / Q000187:drug effects / Q000502:physiology; D053840:Brugada Syndrome; D000075224:Cardiac Conduction System Disease; D006325:Heart Atria / Q000187:drug effects*; D006329:Heart Conduction System / Q000002:abnormalities / Q000187:drug effects / Q000503:physiopathology; D008297:Male; D008839:Microelectrodes; D009200:Myocardial Contraction / Q000187:drug effects* / Q000502:physiology; D011817:Rabbits; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D014807:Vitamin D; C097949:1,25-dihydroxyvitamin D
keywords: acetylcholine; atrial fibrillation; heart failure; left atrium; vitamin D