title: Efficient foreign gene expression in planta using a plantago asiatica mosaic virus-based vector achieved by the strong RNA-silencing suppressor activity of TGBp1.
abstract: Plant virus expression vectors provide a powerful tool for basic research as well as for practical applications. Here, we report the construction of an expression vector based on plantago asiatica mosaic virus (PlAMV), a member of the genus Potexvirus. Modification of a vector to enhance the expression of a foreign gene, combined with the use of the foot-and-mouth disease virus 2A peptide, allowed efficient expression of the foreign gene in two model plant species, Arabidopsis thaliana and Nicotiana benthamiana. Comparison with the widely used potato virus X (PVX) vector demonstrated that the PlAMV vector retains an inserted foreign gene for a longer period than PVX. Moreover, our results showed that the GFP expression construct PlAMV-GFP exhibits stronger RNA silencing suppression activity than PVX-GFP, which is likely to contribute to the stability of the PlAMV vector.
authors.affiliation: Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
authors.forename: Nami
authors.initials: N
authors.lastname: Minato
authors.forename: Ken
authors.initials: K
authors.lastname: Komatsu
authors.forename: Yukari
authors.initials: Y
authors.lastname: Okano
authors.forename: Kensaku
authors.initials: K
authors.lastname: Maejima
authors.forename: Johji
authors.initials: J
authors.lastname: Ozeki
authors.forename: Hiroko
authors.initials: H
authors.lastname: Senshu
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Takahashi
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Yamaji
authors.forename: Shigetou
authors.initials: S
authors.lastname: Namba
mesh_terms: D017360:Arabidopsis / Q000821:virology*; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D010935:Plant Diseases / Q000821:virology; D017863:Potexvirus / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D014026:Nicotiana / Q000821:virology*; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D014764:Viral Proteins; D049452:Green Fluorescent Proteins