title: Perfluorooctanesulfonate (PFOS) perturbs male rat Sertoli cell blood-testis barrier function by affecting F-actin organization via p-FAK-Tyr(407): an in vitro study.
abstract: Environmental toxicants such as perfluorooctanesulfonate (PFOS) have been implicated in male reproductive dysfunction, including reduced sperm count and semen quality, in humans. However, the underlying mechanism(s) remains unknown. Herein PFOS at 10-20 μM (∼5-10 μg/mL) was found to be more potent than bisphenol A (100 μM) in perturbing the blood-testis barrier (BTB) function by disrupting the Sertoli cell tight junction-permeability barrier without detectable cytotoxicity. We also delineated the underlying molecular mechanism by which PFOS perturbed Sertoli cell BTB function using an in vitro model that mimics the BTB in vivo. First, PFOS perturbed F-actin organization in Sertoli cells, causing truncation of actin filaments at the BTB. Thus, the actin-based cytoskeleton was no longer capable of supporting the distribution and/or localization of actin-regulatory and adhesion proteins at the cell-cell interface necessary to maintain BTB integrity. Second, PFOS was found to perturb inter-Sertoli cell gap junction (GJ) communication based on a dye-transfer assay by down-regulating the expression of connexin-43, a GJ integral membrane protein. Third, phosphorylated focal adhesion kinase (FAK)-Tyr(407) was found to protect the BTB from the destructive effects of PFOS as shown in a study via an overexpression of an FAK Y407E phosphomimetic mutant. Also, transfection of Sertoli cells with an FAK-specific microRNA, miR-135b, to knock down the expression of phosphorylated FAK-Tyr(407) was found to worsen PFOS-mediated Sertoli cell tight junction disruption. In summary, PFOS-induced BTB disruption is mediated by down-regulating phosphorylated FAK-Tyr(407) and connexin-43, which in turn perturbed F-actin organization and GJ-based intercellular communication, leading to mislocalization of actin-regulatory and adhesion proteins at the BTB.
authors.affiliation: The Mary M. Wohlford Laboratory for Male Contraceptive Research (H.-T.W., D.D.M., C.Y.C.), Center for Biomedical Research, Population Council, New York, New York 10065; and Department of Biology (C.K.C.W.), Hong Kong Baptist University, Kowloon, Hong Kong, China.
authors.forename: Hin-Ting
authors.initials: HT
authors.lastname: Wan
authors.forename: Dolores D
authors.initials: DD
authors.lastname: Mruk
authors.forename: Chris K C
authors.initials: CK
authors.lastname: Wong
authors.forename: C Yan
authors.initials: CY
authors.lastname: Cheng
mesh_terms: D000199:Actins / Q000378:metabolism*; D017738:Alkanesulfonic Acids / Q000737:chemistry*; D000818:Animals; D001559:Benzhydryl Compounds / Q000737:chemistry; D001814:Blood-Testis Barrier / Q000187:drug effects / Q000378:metabolism*; D002450:Cell Communication; D018031:Connexin 43 / Q000378:metabolism; D015536:Down-Regulation; D005466:Fluorocarbons / Q000737:chemistry*; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism*; D017629:Gap Junctions / Q000378:metabolism; D020868:Gene Silencing; D008297:Male; D008854:Microscopy, Electron; D009154:Mutation; D010539:Permeability; D010636:Phenols / Q000737:chemistry; D051381:Rats; D012708:Sertoli Cells / Q000187:drug effects / Q000378:metabolism*; D019108:Tight Junctions / Q000378:metabolism; D014443:Tyrosine / Q000737:chemistry*; D000098826:Bisphenol A Compounds
chemical_list: D000199:Actins; D017738:Alkanesulfonic Acids; D001559:Benzhydryl Compounds; D018031:Connexin 43; D005466:Fluorocarbons; D051416:Focal Adhesion Protein-Tyrosine Kinases; D010636:Phenols; D014443:Tyrosine; C076994:perfluorooctane sulfonic acid; C006780:bisphenol A; D000098826:Bisphenol A Compounds