title: A new insect cell line from the pupal ovary of the Asian corn borer moth Ostrinia furnacalis.
abstract: Ovaries were removed from Ostrinia furnacalis (Guenée) pupae and were placed in a flask containing TNM-FH medium with 10% inactivated fetal bovine serum. Cell migration occurred after about 1 wk of the initiation in June 2011. The migrated cells were distributed over most of the flask and were treated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), a chemical carcinogen, after about 1 mo of initiation for 26 d. Cells were first subcultured successfully 12 d after the MNNG was removed, followed by subculturing for 30 passages. The established cell line, designated IOZCAS-Osfu-1, were analyzed by DNA fingerprinting-PCR (DAF-PCR) to confirm that it originated from O. furnacalis.
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences (CAS), Beichenxilu 1-5, Beijing, 100101, China, 13964113243@163.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Qilian
authors.initials: Q
authors.lastname: Qin
authors.forename: Huan
authors.initials: H
authors.lastname: Gong
authors.forename: Qian
authors.initials: Q
authors.lastname: Meng
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.forename: Menglong
authors.initials: M
authors.lastname: Wang
authors.forename: Jihong
authors.initials: J
authors.lastname: Zhang
authors.forename: Guiling
authors.initials: G
authors.lastname: Zhou
authors.forename: Xuan
authors.initials: X
authors.lastname: Li
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D016172:DNA Fingerprinting; D005260:Female; D008769:Methylnitronitrosoguanidine; D009036:Moths / Q000166:cytology*; D010053:Ovary / Q000166:cytology; D011679:Pupa / Q000166:cytology
chemical_list: D008769:Methylnitronitrosoguanidine