title: Joint instability and cartilage compression in a mouse model of posttraumatic osteoarthritis.
abstract: Joint instability and cartilage trauma have been previously studied and identified as key mediators in the development of posttraumatic osteoarthritis (PTOA). The purpose of this study was to use an in vivo model to compare the effect of joint instability, caused by the rupture of the anterior cruciate ligament (ACL), versus cartilage compression. In this study, mice were subjected to cyclical axial loads of twelve Newtons (N) for 240 cycles or until the ACL ruptured. One and eight weeks after this procedure, knees were sectioned coronally and evaluated for osteoarthritis by histology. Using a scoring scale established by [Pritzker K, Gay S, Jimenez S, et al. (2006): Osteoarthritis Cartilage 14:13-29], the articular cartilage across each surface was scored and combined to produce a total degeneration score. The ACL-ruptured group had a significantly greater total degeneration score than either control or compression treated joints at 1 and 8 weeks. Additionally, only sections from ACL-ruptured knees consistently showed synovitis after 1 week and osteophyte formation after 8 weeks. Thus, it appears using that ACL rupture consistently creates a severe osteoarthritis phenotype, while axial cartilage compression alone does not appear to be an appropriate method of inducing PTOA in vivo.
authors.affiliation: Department of Orthopaedic Surgery, San Francisco Veterans Affairs Medical Center, San Francisco, California.
authors.forename: Tarik S
authors.initials: TS
authors.lastname: Onur
authors.forename: Ruobin
authors.initials: R
authors.lastname: Wu
authors.forename: Stacey
authors.initials: S
authors.lastname: Chu
authors.forename: Wenhan
authors.initials: W
authors.lastname: Chang
authors.forename: Hubert T
authors.initials: HT
authors.lastname: Kim
authors.forename: Alexis B C
authors.initials: AB
authors.lastname: Dang
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000473:pathology; D000070598:Anterior Cruciate Ligament Injuries; D002358:Cartilage, Articular / Q000473:pathology*; D004195:Disease Models, Animal; D007593:Joint Instability / Q000209:etiology / Q000473:pathology*; D008297:Male; D051379:Mice; D010003:Osteoarthritis / Q000209:etiology* / Q000473:pathology; D054850:Osteophyte; D012421:Rupture / Q000473:pathology
keywords: anterior cruciate ligament; cartilage; compression; mouse model; posttraumatic osteoarthritis