title: Postelectrospinning modifications for alginate nanofiber-based wound dressings.
abstract: Alginate nanofibers have been attractive for potential tissue regeneration applications due to a combination of their moisture retention ability and large surface area available in a nonwoven nanofiber form. This study aims to address several challenges in alginate nanofiber application, including the lack of structural stability in aqueous environment and limited cell attachment as compared to commercial wound dressings, via examining crosslinking techniques. In addition to the commonly performed divalent ion crosslinking, a glutaraldehyde double-crosslinking step and polylysine addition were applied to an electrospun alginate nanofiber nonwoven mat. With optimization of the electrospinning solution, nanofiber morphology was maintained after the two-stage crosslinking process. Extensibility of the nanofiber mat reduced after the crosslinking process. However, both aqueous stability and cell attachment improved after the postspinning modifications, as shown through degradation tests in phosphate buffered saline solutions and fibroblast cell culture studies, respectively.
authors.affiliation: Advanced Fibrous Materials Laboratory, Department of Materials Engineering, University of British Columbia, British Columbia, Canada.
authors.forename: Victor
authors.initials: V
authors.lastname: Leung
authors.forename: Ryan
authors.initials: R
authors.lastname: Hartwell
authors.forename: Sanam Salimi
authors.initials: SS
authors.lastname: Elizei
authors.forename: Heejae
authors.initials: H
authors.lastname: Yang
authors.forename: Aziz
authors.initials: A
authors.lastname: Ghahary
authors.forename: Frank
authors.initials: F
authors.lastname: Ko
mesh_terms: D020341:Absorbable Implants; D000464:Alginates / Q000737:chemistry*; D001458:Bandages; D002118:Calcium / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003432:Cross-Linking Reagents; D005347:Fibroblasts / Q000187:drug effects; D020723:Glucuronic Acid / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers; D011092:Polyethylene Glycols / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry; D012964:Sodium / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013500:Surface Tension; D013718:Tensile Strength; D014783:Viscosity; D014945:Wound Healing; D014947:Wounds and Injuries / Q000628:therapy*
chemical_list: D000464:Alginates; D003432:Cross-Linking Reagents; D006603:Hexuronic Acids; D011107:Polylysine; D011092:Polyethylene Glycols; D020723:Glucuronic Acid; D012964:Sodium; D002118:Calcium; D005976:Glutaral
keywords: electrospinning; material processing; nanofibers; tissue regeneration; wound healing