title: Identification of elaiophylin derivatives from the marine-derived actinomycete Streptomyces sp. 7-145 using PCR-based screening.
abstract: A PCR-based genetic screening experiment targeting the dTDP-glucose-4,6-dehydratase gene revealed that a marine sediment-derived strain, Streptomyces sp. 7-145, had the potential to produce glycosidic antibiotics. Chemical investigation of culture extracts of this strain yielded two new 6-deoxyhexose-containing antibiotics, 11',12'-dehydroelaiophylin (1) and 11,11'-O-dimethyl-14'-deethyl-14'-methylelaiophylin (2), together with four known elaiophylin analogues (3-6). Their structures were determined by extensive NMR, MS, and CD analyses. Compounds 1, 3, 4, and 6 showed good antibacterial activity against methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci pathogens.
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing 100050, People's Republic of China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Wu
authors.forename: Yi
authors.initials: Y
authors.lastname: Tan
authors.forename: Maoluo
authors.initials: M
authors.lastname: Gan
authors.forename: Yiguang
authors.initials: Y
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Guan
authors.forename: Xinxin
authors.initials: X
authors.lastname: Hu
authors.forename: Hongxia
authors.initials: H
authors.lastname: Zhou
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Shang
authors.forename: Xuefu
authors.initials: X
authors.lastname: You
authors.forename: Zhaoyong
authors.initials: Z
authors.lastname: Yang
authors.forename: Chunling
authors.initials: C
authors.lastname: Xiao
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018942:Macrolides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008386:Marine Biology; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D016133:Polymerase Chain Reaction; D013302:Streptomyces / Q000737:chemistry*; D014640:Vancomycin / Q000494:pharmacology
chemical_list: C586160:11',12'-dehydroelaiophylin; C586161:11,11'-O-dimethyl-14'-deethyl-14'-methylelaiophylin; D000900:Anti-Bacterial Agents; D018942:Macrolides; C033279:elaiophylin; D014640:Vancomycin