title: The fate of Krüppel-like factor 9-positive hepatic carcinoma cells may be determined by the programmed cell death protein 5.
abstract: Liver cancer in men is the fifth most frequently diagnosed cancer worldwide. Human Krüppel-like factor (KLF9) gene, localized on human chromosome 9q13, has been implicated in mediating a diverse range of biological processes including stem cell maintenance and differentiation of T- and B-lymphocytes. In this study, we confirmed that the levels of KLF9 mRNA and protein were lower in hepatocellular carcinoma (HCC) tissue compared to normal tissue. In addition, we confirmed that upregulation of KLF9 inhibited cell proliferation and mobility and induce apoptosis in HepG2 cells depending on programmed cell death protein 5 (PDCD5) expression. However, no interaction was found between KLF9 and PDCD5 using fluorescence resonance energy transfer (FRET) and co-immunoprecipitation (co-IP). We confirmed that PDCD5 overexpression stimulated the promoter activities of KLF9 by luciferase reporter assays.
authors.affiliation: Department of General Surgery, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, P.R. China.
authors.forename: Da-Zhi
authors.initials: DZ
authors.lastname: Fu
authors.forename: Ying
authors.initials: Y
authors.lastname: Cheng
authors.forename: Hui
authors.initials: H
authors.lastname: He
authors.forename: Hai-Yang
authors.initials: HY
authors.lastname: Liu
authors.forename: Yong-Feng
authors.initials: YF
authors.lastname: Liu
mesh_terms: D017209:Apoptosis / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis* / Q000235:genetics; D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D002454:Cell Differentiation; D049109:Cell Proliferation; D002899:Chromosomes, Human, Pair 9; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism*; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology
chemical_list: D051017:Apoptosis Regulatory Proteins; C489676:KLF9 protein, human; D051741:Kruppel-Like Transcription Factors; D009363:Neoplasm Proteins; C117283:PDCD5 protein, human; D012333:RNA, Messenger