title: Age-related differences in the biological parameters of vertebral cancellous bone from Chinese women.
abstract: BACKGROUND
  With aging, the human fracture risks gradually increase. This is mainly due to the corresponding changes of the biomechanical parameters of human bone presents with aging. We measured the microstructural parameters of lumbar bone from women in several age groups by micro-computed tomography and scanning electron microscopy. We observed changes in lumbar cancellous bone mineral density and in biomechanical parameters with aging to elucidate the relationship between age and risk of fracture. We provide theoretical support for human pathology, fracture risk increased with age and the individualized of each age group.
  
  
  METHODS
  Thirty-two fresh L3 vertebral bodies were donated from 32 women, aged 20-59 years and were divided into four age groups: 20 to 29 years (group A); 30 to 39 years (group B); 40 to 49 years (group C); and 50 to 59 years (group D). Conventional lumbar separation was performed by removing soft tissue and subsidiary structures, leaving only the vertebral body. The vertebral body was cut into halves along the median sagittal plane, maintaining the upper and lower end-plates of each half, and used for biomechanical, morphological, and density measurements.
  
  
  RESULTS
  Comparing group A to B, the rod-like trabecular thickness (Tb.Th) decreased; the trabecular spacing (Tb.Sp) increased; the plate-like Tb.Th decreased; bone mineral density, tissue mineral density, bone volume fraction, and bone surface fraction decreased, and the elastic modulus and the ultimate stress decreased (all changes P < 0.05). Similar significant (P < 0.05) trends were obtained when comparing group C to D. With aging, the collagen cross-linking capacity declined, the thickness of the collagen fibrils was variable (ranging from almost the same to loose, sparse, or disordered), and the finer collagen fibrils between the thick filaments were disorganized.
  
  
  CONCLUSIONS
  In women aged 20 to 59 years, the rod-like and plate-like Tb.Th of the vertebral body decreased, while Tb.Sp increased. Additionally, the density, elastic modulus, and ultimate stress of the cancellous bone decreased with age. These associated changes in bone microstructure, density, and biomechanics with age may lead to an increasing risk of osteoporosis and fracture.
authors.affiliation: Department of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun, Jinlin 130041, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Jiang
authors.forename: Guo-min
authors.initials: GM
authors.lastname: Liu
authors.forename: Hao-tian
authors.initials: HT
authors.lastname: Bai
authors.forename: Tian-bin
authors.initials: TB
authors.lastname: Wang
authors.forename: Han
authors.initials: H
authors.lastname: Wu
authors.forename: Yun-yan
authors.initials: YY
authors.lastname: Jia
authors.forename: Yun-gang
authors.initials: YG
authors.lastname: Luo
mesh_terms: D000328:Adult; D015519:Bone Density / Q000502:physiology*; D005260:Female; D006801:Humans; D008159:Lumbar Vertebrae / Q000378:metabolism* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D055114:X-Ray Microtomography; D055815:Young Adult