title: Direct regenerationin vitro and transient GUS expression inMentha xpiperita.
abstract: Genetic transformation of peppermint is known to be very difficult essentially because of low efficiency regeneration. A regeneration protocol allowing 51% shooting frequency is proposed. Transient β-glucuronidase expression and adjustment of selection pressure with kanamycin are also reported. The final retained method to attempt peppermint transformation is:Agrobacterium inoculation or biolistic treatment of the first apical leaves ofin vitro clones, regeneration in the dark with kanamycin (1 mg l(-1)) and 6-benzylaminopurine (2 mg l(-1)), followed by selection of regenerated shoots with 200 mg 1(-1) kanamycin.
authors.affiliation: Laboratoire de Biotechnologies Appliquées aux Plantes Aromatiques, Faculté des Sciences et Techniques, Université Jean Monnet, 23 rue du Dr Michelon, 42023, Saint-Etienne Cédex 2, France.
authors.forename: J C
authors.initials: JC
authors.lastname: Caissard
authors.forename: O
authors.initials: O
authors.lastname: Faure
authors.forename: F
authors.initials: F
authors.lastname: Jullien
authors.forename: M
authors.initials: M
authors.lastname: Colson
authors.forename: A
authors.initials: A
authors.lastname: Perrin