title: Reduction of oxidative stress may play a role in the anti-inflammatory effect of the novel herbal formulation in a rat model of hydrochloric acid-induced cystitis.
abstract: OBJECTIVE
  We investigated the effect of the multi-herbal medicine, WSY-1075 in an animal model of hydrochloric acid (HCl)-induced cystitis.
  
  
  METHODS
  Rats were randomly assigned to three groups: sham-operated (control), HCl-induced only (HC), and HC treated with WSY-1075 (HC + WT). Oral administration of either distilled water (control, HC) or WSY-1075 (400 mg/kg) was continued for 4 weeks. In HC and HC + WT groups, cystitis was induced with 0.4 M HCl beginning on the 22nd day. Rats in each group underwent cystometrography, and bladders were examined for evidence of inflammation and oxidative stress.
  
  
  RESULTS
  Treatment with WSY-1075 decreased the frequency of urination and reduced inflammation of the bladder tissue in a rat model of HCl-induced cystitis. Compared with the control group, the HC group showed severe chronic inflammatory and fibrosis signs, and the inflammatory grades significantly decreased following WSY-1075 treatment in the HC-WT group. The HC + WT group showed a markedly decreased expression of pro-inflammatory cytokines compared to the HC group. The level of malondialdehyde was significantly greater in the HC group compared to the control group, and it was significantly reduced in the treated (HC + WT) group. The levels of superoxide dismutase increased in the HC + WT group, which confirmed the anti-oxidant effect of WSY-1075.
  
  
  CONCLUSIONS
  We suggest that reduction of oxidative stress may play a role in this anti-inflammatory effect.
authors.affiliation: Department of Urology, The Catholic University of Korea, College of Medicine, Seoul, Korea.
authors.forename: Woong-Jin
authors.initials: WJ
authors.lastname: Bae
authors.forename: U-Syn
authors.initials: US
authors.lastname: Ha
authors.forename: Seol
authors.initials: S
authors.lastname: Kim
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Sung-Hoo
authors.initials: SH
authors.lastname: Hong
authors.forename: Ji-Youl
authors.initials: JY
authors.lastname: Lee
authors.forename: Tae-Kon
authors.initials: TK
authors.lastname: Hwang
authors.forename: Sung-Yeoun
authors.initials: SY
authors.lastname: Hwang
authors.forename: Hong-Jun
authors.initials: HJ
authors.lastname: Kim
authors.forename: Sae-Woong
authors.initials: SW
authors.lastname: Kim
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D003556:Cystitis / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D006851:Hydrochloric Acid; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D001743:Urinary Bladder / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; C546310:Gongjin-dan; D010936:Plant Extracts; D013482:Superoxide Dismutase; D006851:Hydrochloric Acid
keywords: anti-oxidants; cystitis; inflammation; interstitial