title: New guar biopolymer silver nanocomposites for wound healing applications.
abstract: Wound healing is an innate physiological response that helps restore cellular and anatomic continuity of a tissue. Selective biodegradable and biocompatible polymer materials have provided useful scaffolds for wound healing and assisted cellular messaging. In the present study, guar gum, a polymeric galactomannan, was intrinsically modified to a new cationic biopolymer guar gum alkylamine (GGAA) for wound healing applications. Biologically synthesized silver nanoparticles (Agnp) were further impregnated in GGAA for extended evaluations in punch wound models in rodents. SEM studies showed silver nanoparticles well dispersed in the new guar matrix with a particle size of ~18 nm. In wound healing experiments, faster healing and improved cosmetic appearance were observed in the new nanobiomaterial treated group compared to commercially available silver alginate cream. The total protein, DNA, and hydroxyproline contents of the wound tissues were also significantly higher in the treated group as compared with the silver alginate cream (P < 0.05). Silver nanoparticles exerted positive effects because of their antimicrobial properties. The nanobiomaterial was observed to promote wound closure by inducing proliferation and migration of the keratinocytes at the wound site. The derivatized guar gum matrix additionally provided a hydrated surface necessary for cell proliferation.
authors.affiliation: Department of Chemical Technology, University of Calcutta, 92 A.P.C. Road, Kolkata, West Bengal 700009, India.
authors.forename: Runa
authors.initials: R
authors.lastname: Ghosh Auddy
authors.forename: Md Farooque
authors.initials: MF
authors.lastname: Abdullah
authors.forename: Suvadra
authors.initials: S
authors.lastname: Das
authors.forename: Partha
authors.initials: P
authors.lastname: Roy
authors.forename: Sriparna
authors.initials: S
authors.lastname: Datta
authors.forename: Arup
authors.initials: A
authors.lastname: Mukherjee
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000627:therapeutic use / Q000633:toxicity; D005685:Galactans / Q000737:chemistry / Q000627:therapeutic use; D006097:Granulation Tissue / Q000187:drug effects / Q000473:pathology; D008297:Male; D008351:Mannans / Q000737:chemistry / Q000627:therapeutic use; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry / Q000627:therapeutic use* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry / Q000627:therapeutic use* / Q000648:ultrastructure; D053149:Plant Gums / Q000737:chemistry / Q000627:therapeutic use; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D012834:Silver / Q000008:administration & dosage / Q000627:therapeutic use* / Q000633:toxicity; D013053:Spectrophotometry; D013718:Tensile Strength; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D005685:Galactans; D008351:Mannans; D053149:Plant Gums; D012834:Silver; C007894:guar gum