title: Dynamic anchoring of the 3'-end of the guide strand controls the target dissociation of Argonaute-guide complex.
abstract: Argonaute (Ago) is the catalytic core of small RNA-based gene regulation. Despite plenty of mechanistic studies on Ago, the dynamical aspects and the mechanistic determinants of target mRNA binding and dissociation of Ago-guide strand remain unclear. Here, by using single-molecule fluorescence resonance energy transfer (FRET) assays and Thermus thermophilus Ago (TtAgo), we reveal that the 3'-end of the guide strand dynamically anchors at and releases from the PAZ domain of Ago, and that the 3'-end anchoring of the guide strand greatly accelerates the target dissociation by destabilizing the guide-target duplex. Our results indicate that the target binding/dissociation of Ago-guide is executed through the dynamic interplays among Ago, guide, and target.
authors.affiliation: Department of Physics and Astronomy, ‡National Center for Creative Research Initiatives, and §Department of Biophysics and Chemical Biology, Seoul National University , Seoul 151-747, Korea.
authors.forename: Seung-Ryoung
authors.initials: SR
authors.lastname: Jung
authors.forename: Eunji
authors.initials: E
authors.lastname: Kim
authors.forename: Wonseok
authors.initials: W
authors.lastname: Hwang
authors.forename: Soochul
authors.initials: S
authors.lastname: Shin
authors.forename: Ji-Joon
authors.initials: JJ
authors.lastname: Song
authors.forename: Sungchul
authors.initials: S
authors.lastname: Hohng
mesh_terms: D060565:Argonaute Proteins / Q000737:chemistry / Q000378:metabolism*; D001483:Base Sequence; D031541:Fluorescence Resonance Energy Transfer; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D016963:Thermus thermophilus / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism
chemical_list: D060565:Argonaute Proteins; D012333:RNA, Messenger