title: Effect of culture medium type on canine adipose-derived mesenchymal stem cells and developmental competence of interspecies cloned embryos.
abstract: Canine adipose-derived mesenchymal stem cells (ASCs) are promising as donor cells for somatic cell nuclear transfer (SCNT). It has been suggested that different cell cultures possess different capacities to support pre-implantation development of SCNT embryos. The aim of this study is to investigate whether two culture medium (RCMEP, Dulbecco's modified Eagle's medium [DMEM]) affect gene expression of ASCs, subsequent development of interspecies SCNT (iSCNT) and gene expression of cloned embryos. The RCMEP-cultured cells contained significantly greater amounts of SOX2, NANOG, OCT4, DNMT1, and MeCP2 than DMEM-cultured cells (P < 0.05). In iSCNT, the use of DMEM medium for culturing cells resulted in similar development to the blastocyst stage than those derived from RCMEP cultured cells (4.5% and 3.2%, respectively; P > 0.05). The expression of all transcripts except for DNMT1 in cloned blastocysts from RCMEP cultured cells followed those of cloned blastocysts derived from DMEM cultured cells. The alteration of gene expression in ASCs by culture medium was not manifested in the iSCNT embryos derived from these cells. Although the culture medium can induce changes of gene expression by ASCs, such alterations in donor cells did not affect the developmental competence or gene expression patterns of iSCNT embryos.
authors.affiliation: Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
authors.forename: Geon A
authors.initials: GA
authors.lastname: Kim
authors.affiliation: Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Oh
authors.affiliation: College of Natural and Mathematical Sciences, University of Maryland, Baltimore County, Baltimore, Maryland, USA.
authors.forename: Tae Hee
authors.initials: TH
authors.lastname: Lee
authors.affiliation: College of Arts and Science, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: College of Carnegie Institute of Technology, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA.
authors.forename: Sang Hwan
authors.initials: SH
authors.lastname: Oh
authors.affiliation: Department of Electrical and Computer Engineering, University of British Columbia, Vancouver, Canada.
authors.forename: Ju Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
authors.forename: Jin Wook
authors.initials: JW
authors.lastname: Kim
authors.affiliation: College of Engineering, University of Michigan, Ann Arbor, Michigan, USA.
authors.forename: Se Woon
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea. Electronic address: bclee@snu.ac.kr.
authors.forename: Byeong Chun
authors.initials: BC
authors.lastname: Lee
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000818:Animals; D018929:Cell Culture Techniques / Q000662:veterinary; D002454:Cell Differentiation; D002478:Cells, Cultured; D019976:Cloning, Organism / Q000379:methods / Q000662:veterinary*; D003470:Culture Media; D004285:Dogs; D047108:Embryonic Development; D020869:Gene Expression Profiling; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D053652:Nuclear Transfer Techniques / Q000662:veterinary*
chemical_list: D003470:Culture Media
keywords: Canine adipose-derived mesenchymal stem cells; DMEM; Gene expression; RCMEP; iSCNT; iSCNT blastocyst