title: The first outbreak of giardiasis with drinking water in Korea.
abstract: OBJECTIVE
  To identify the pathogen of the diarrhea outbreak in a village in Jeollabuk province in Korea in April 2010.
  
  
  METHODS
  DNA extraction was performed from the 120 L of collected water, which was centrifuged at 10,000 x g for 30 min. PCR reactions were conducted in a total of 25 ul, which included PCR premix (GenDEPOT, Barker, TX, USA), 2 ul (∼100 ng) of extracted DNA, and 10 pmol of each primer.
  
  
  RESULTS
  Nine people out of 25 had a symptom of abdominal pain accompanied by diarrhea after they used stored valley water in a water tank as a provisional water supply source without chlorine sterilization. Among them Giardia lamblia was detected in fecal samples of 7 people using the polymerase chain reaction method. Although G. lamblia was also detected from water provided by the provisional water supply system stored in the water tank and used as drinking water, it was not detected in the water tank itself. This water-borne outbreak is considered to have occurred when the provisional water supply tube was destroyed under a building construction and contaminated by G. lamblia, but its precise cause has not been clarified.
  
  
  CONCLUSIONS
  This outbreak resulting from G. lamblia is very meaningful as the first outbreak of an infection by a water-borne parasite in Korea.
authors.affiliation: Division of Malaria and Parasitic Diseases, Korea National Institute of Health, Osong, Korea. ; Division of Epidemic Intelligence Service, Korea Centers for Diseases Control and Prevention, Osong, Korea.
authors.forename: Hyeng-Il
authors.initials: HI
authors.lastname: Cheun
authors.forename: Cheon-Hyeon
authors.initials: CH
authors.lastname: Kim
authors.forename: Shin-Hyeong
authors.initials: SH
authors.lastname: Cho
authors.forename: Da-Won
authors.initials: DW
authors.lastname: Ma
authors.forename: Bo-La
authors.initials: BL
authors.lastname: Goo
authors.forename: Mun-Su
authors.initials: MS
authors.lastname: Na
authors.forename: Seung-Ki
authors.initials: SK
authors.lastname: Youn
authors.forename: Won-Ja
authors.initials: WJ
authors.lastname: Lee
keywords: Giardia lamblia; giardiasis; water-borne outbreak