title: DNA methylation in mouse testes.
abstract: DNA methylation of retrotransposons and imprinted genes is accurately regulated in spermatogenesis. In particular, CpG methylation of long interspersed elements-1 (LINE1 or L1) and intracisternal A-particle (IAP) retrotransposons during spermatogenesis has been well characterized. CpG methylation of the regulatory regions of retrotransposons is acquired during embryonic testis development; however, reductions of DNA methylation in LINE1 and/or IAP and/or Rasgrf1, which is an imprinted gene, are observed in deficient mice of piRNA biogenesis concerning. Here, we describe two methods, bisulfite sequencing and Southern blotting using a methylation-sensitive restriction enzyme, for analysis of DNA methylation of LINE1, IAP, and imprinted genes in mouse testes.
authors.affiliation: Department of Pathology, Medical School, Osaka University, Osaka, Japan.
authors.forename: Satomi
authors.initials: S
authors.lastname: Kuramochi-Miyagawa
authors.forename: Kanako
authors.initials: K
authors.lastname: Kita-Kojima
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Shiromoto
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ito
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Koshima
authors.forename: Toru
authors.initials: T
authors.lastname: Nakano
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D015820:Cadherins / Q000276:immunology; D019175:DNA Methylation; D005434:Flow Cytometry; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D017422:Sequence Analysis, DNA; D013091:Spermatogenesis / Q000235:genetics*; D013447:Sulfites / Q000494:pharmacology; D013737:Testis / Q000166:cytology / Q000196:embryology / Q000378:metabolism*
chemical_list: D015820:Cadherins; D050814:Octamer Transcription Factor-3; D013447:Sulfites; C042345:hydrogen sulfite