title: Antibiotic resistance genes in the bacteriophage DNA fraction of human fecal samples.
abstract: A group of antibiotic resistance genes (ARGs) (blaTEM, blaCTX-M-1, mecA, armA, qnrA, and qnrS) were analyzed by real-time quantitative PCR (qPCR) in bacteriophage DNA isolated from feces from 80 healthy humans. Seventy-seven percent of the samples were positive in phage DNA for one or more ARGs. blaTEM, qnrA, and, blaCTX-M-1 were the most abundant, and armA, qnrS, and mecA were less prevalent. Free bacteriophages carrying ARGs may contribute to the mobilization of ARGs in intra- and extraintestinal environments.
authors.affiliation: Department of Microbiology, University of Barcelona, Barcelona, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Quirós
authors.forename: Marta
authors.initials: M
authors.lastname: Colomer-Lluch
authors.forename: Alexandre
authors.initials: A
authors.lastname: Martínez-Castillo
authors.forename: Elisenda
authors.initials: E
authors.lastname: Miró
authors.forename: Marc
authors.initials: M
authors.lastname: Argente
authors.forename: Juan
authors.initials: J
authors.lastname: Jofre
authors.forename: Ferran
authors.initials: F
authors.lastname: Navarro
authors.forename: Maite
authors.initials: M
authors.lastname: Muniesa
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001435:Bacteriophages / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005243:Feces / Q000382:microbiology; D006801:Humans
chemical_list: D000900:Anti-Bacterial Agents; D004279:DNA, Viral