title: Early postnatal repeated maternal deprivation causes a transient increase in OMpg and BDNF in rat cerebellum suggesting precocious myelination.
abstract: Repetitive maternal deprivation (MD) of neonatal rats during early life is known as one of the strongest stressors to pre-weaned animals. There is increasing evidence that the cerebellum is involved in cognition and emotion. In the present study, we examined how neurotrophic factors and myelin-associated molecules and their receptors (NGF, BDNF, OMgp, TrkA, TrkB, p75 NTR, and NgR) in the cerebellum are affected by early postnatal maternal separation. Rat pups were separated from their mothers for 3h/day during postnatal days (PND) 10-15. At PND 16 and 30, the levels of mRNA and protein in the cerebellum were determined using real-time PCR and Western blot analysis. Cerebellar mRNA and protein levels of BDNF, TrkB, and OMgp were significantly increased in MD rats at PND 16. However, by PND 30 these variables normalized to control levels. In contrast, the levels of mRNA and protein for NGF, TrkA, p75 NTR, and NgR were unchanged at both ages examined. Transient enhancement of neurotrophic system and myelin-associated molecule expression may cause interference of normal development of the cerebellum such as precocious myelination, which may lead to functional and cognitive deficits later in life.
authors.affiliation: Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kagawa 761-0793, Japan. Electronic address: mikit@med.kagawa-u.ac.jp.
authors.forename: Takanori
authors.initials: T
authors.lastname: Miki
authors.affiliation: Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University, Kobe, 657-8501, Japan.
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Yokoyama
authors.affiliation: Department of Pediatrics; Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kagawa 761-0793, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kusaka
authors.affiliation: Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kagawa 761-0793, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Suzuki
authors.affiliation: Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kagawa 761-0793, Japan.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Ohta
authors.affiliation: Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kagawa 761-0793, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Warita
authors.affiliation: Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kagawa 761-0793, Japan.
authors.forename: Zhi-Yu
authors.initials: ZY
authors.lastname: Wang
authors.affiliation: Department of Anesthesia, Nishiwaki Municipal Hospital, 652-1 Shimotoda, Nishiwaki, Hyogo 677-0043, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Ueki
authors.affiliation: Department of Medical Education, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kagawa 761-0793, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Sumitani
authors.affiliation: Cell and Molecular Biology Department, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI 96813, USA.
authors.forename: Frederick P
authors.initials: FP
authors.lastname: Bellinger
authors.affiliation: Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kagawa 761-0793, Japan.
authors.forename: Motoki
authors.initials: M
authors.lastname: Tamai
authors.affiliation: Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kagawa 761-0793, Japan.
authors.forename: Jun-Qian
authors.initials: JQ
authors.lastname: Liu
authors.affiliation: Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kagawa 761-0793, Japan.
authors.forename: Tomiko
authors.initials: T
authors.lastname: Yakura
authors.affiliation: Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kagawa 761-0793, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Takeuchi
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis*; D002531:Cerebellum / Q000254:growth & development / Q000378:metabolism*; D005260:Female; D058851:GPI-Linked Proteins / Q000096:biosynthesis*; D008297:Male; D008426:Maternal Deprivation; D009185:Myelin Proteins / Q000096:biosynthesis*; D009413:Nerve Fibers, Myelinated / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D058851:GPI-Linked Proteins; D009185:Myelin Proteins; C560532:Omg protein, rat
keywords: Cerebellum; Development; Maternal deprivation; Myelination; Neurotrophic factor; Stress