title: In vitro study of AFB1 and AFM1 effects on human lymphoblastoid Jurkat T-cell model.
abstract: Aflatoxin B(1) (AFB(1)) is a mycotoxin produced by Aspergillus spp. that can occur as a natural contaminant in foods and feeds of vegetable origin. Post-ingestion, AFB(1) can be metabolized in the liver of mammals into hydroxylated aflatoxin M(1) (AFM(1)) that is excreted with milk. Although several studies have been carried out to evaluate effects of AFB(1) on the immune system, studies regarding AFM(1) are moreover lacking. The aim of the current study was to investigate effects of AFB(1) and AFM(1) on immune function using a lymphoblastoid Jurkat T-cell line as an experimental model. Both AFB(1) and AFM(1) produced significant decreases in Jurkat cell proliferation, whereas only minor effects were noted on interleukin (IL)-2 and interferon (IFN)-γ cytokines mRNA expression in stimulated cells that had been pre-incubated with AFB(1) and AFM(1). Particularly, AFB(1), but not AFM(1), at the highest concentration (50 µM) induced a marked increase in IL-8 mRNA expression. The results of the current study suggested the existence of a concentration threshold for AFB(1) and AFM(1) needed to exert biological activity on cell viability and innate immunity.
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II , Naples , Italy .
authors.forename: D
authors.initials: D
authors.lastname: Luongo
authors.forename: R
authors.initials: R
authors.lastname: Russo
authors.forename: A
authors.initials: A
authors.lastname: Balestrieri
authors.forename: S
authors.initials: S
authors.lastname: Marzocco
authors.forename: P
authors.initials: P
authors.lastname: Bergamo
authors.forename: L
authors.initials: L
authors.lastname: Severino
mesh_terms: D016604:Aflatoxin B1 / Q000737:chemistry / Q000494:pharmacology*; D016607:Aflatoxin M1 / Q000737:chemistry / Q000494:pharmacology*; D001230:Aspergillus / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D006900:Hydroxylation; D007113:Immunity, Innate / Q000187:drug effects; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D007376:Interleukin-2 / Q000235:genetics / Q000378:metabolism; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D019169:Jurkat Cells; D008099:Liver / Q000378:metabolism; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D015854:Up-Regulation
chemical_list: D007376:Interleukin-2; D016209:Interleukin-8; D016607:Aflatoxin M1; D007371:Interferon-gamma; D016604:Aflatoxin B1
keywords: Aflatoxin B1; Jurkat cells; aflatoxin M1