title: Differentiation of neuronal stem cells into motor neurons using electrospun poly-L-lactic acid/gelatin scaffold.
abstract: Neural stem cells (NSCs) provide promising therapeutic potential for cell replacement therapy in spinal cord injury (SCI). However, high increases of cell viability and poor control of cell differentiation remain major obstacles. In this study, we have developed a non-woven material made of co-electrospun fibers of poly L-lactic acid and gelatin with a degradation rate and mechanical properties similar to peripheral nerve tissue and investigated their effect on cell survival and differentiation into motor neuronal lineages through the controlled release of retinoic acid (RA) and purmorphamine. Engineered Neural Stem-Like Cells (NSLCs) seeded on these fibers, with and without the instructive cues, differentiated into β-III-tubulin, HB-9, Islet-1, and choactase-positive motor neurons by immunostaining, in response to the release of the biomolecules. In addition, the bioactive material not only enhanced the differentiation into motor neuronal lineages but also promoted neurite outgrowth. This study elucidated that a combination of electrospun fiber scaffolds, neural stem cells, and controlled delivery of instructive cues could lead to the development of a better strategy for peripheral nerve injury repair.
authors.affiliation: Department of Chemical Engineering, École Polytechnique de Montréal, P.O. Box 6079, Centre-Ville Station, H3C 3A7 Montréal, Québec, Canada; Groupe de Recherche en Science et Technologie Biomédicale (GRSTB), École Polytechnique de Montréal, P.O. Box 6079, Centre-Ville Station, H3C 3A7 Montréal, Québec, Canada.
authors.forename: Loïc
authors.initials: L
authors.lastname: Binan
authors.forename: Charlène
authors.initials: C
authors.lastname: Tendey
authors.forename: Gregory
authors.initials: G
authors.lastname: De Crescenzo
authors.forename: Rouwayda
authors.initials: R
authors.lastname: El Ayoubi
authors.forename: Abdellah
authors.initials: A
authors.lastname: Ajji
authors.forename: Mario
authors.initials: M
authors.lastname: Jolicoeur
mesh_terms: D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D009025:Morpholines / Q000737:chemistry; D009046:Motor Neurons / Q000166:cytology*; D009417:Nerve Tissue / Q000166:cytology / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011687:Purines / Q000737:chemistry; D013119:Spinal Cord Injuries / Q000628:therapy; D054457:Tissue Scaffolds / Q000737:chemistry*; D014212:Tretinoin / Q000737:chemistry
chemical_list: D009025:Morpholines; D011091:Polyesters; D011108:Polymers; D011687:Purines; D019344:Lactic Acid; C033616:poly(lactide); D014212:Tretinoin; D005780:Gelatin; C470893:purmorphamine
keywords: Differentiation; Drug delivery; Electrospinning; Motor neuron; Nerve; Regeneration