title: A gene encoding a truncated large subunit of Rubisco is transcribed and salt-inducible in rice.
abstract: Using the rice salt-tolerant mutant 20 as material, a cDNA library was constructed and two salt-inducible clones, SIR5.5 and SIR8.1, were isolated by differential screening. Homology analysis revealed that the two clones together constituted a chimeric rbcL which encoded a truncated large subunit of Rubisco with 337 amino-acids, plus 64 amino-acids of unknown origin. The expressions of both the normal and the chimeric locus appeared to be developmentally regulated and salt-inducible in shoots of the salt-tolerant mutant 20 and its original variety 77-170. In roots, their expressions were salt-inducible in the salt-tolerant mutant 20 whereas no, or only premature, forms were present in the salt-treated original variety 77-170. Higher concentrations of salt reduced the expressions of both normal rbcL and the chimeric locus. ABA showed no effect on their expression.
authors.affiliation: Plant Biotechnology Laboratory, Institute of Genetics, Academia Sinica, 100101, Beijing, Peoples Republic of China.
authors.forename: J S
authors.initials: JS
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Gu
authors.forename: F H
authors.initials: FH
authors.lastname: Liu
authors.forename: S Y
authors.initials: SY
authors.lastname: Chen