title: Antioxidant properties of Australian canola meal protein hydrolysates.
abstract: Antioxidant activities of canola protein hydrolysates (CPHs) and peptide fractions prepared using five proteases and ultrafiltration membranes (1, 3, 5, and 10kDa) were investigated. CPHs had similar and adequate quantities of essential amino acids. The effective concentration that scavenged 50% (EC50) of the ABTS(+) was greatest for the <1kDa pancreatin fraction at 10.1μg/ml. CPHs and peptide fractions scavenged DPPH(+) with most of the EC50 values being <1.0mg/ml. Scavenging of superoxide radical was generally weak, except for the <1kDa pepsin peptide fraction that had a value of 51%. All CPHs inhibited linoleic acid oxidation with greater efficiency observed for pepsin hydrolysates. The oxygen radical absorbance capacity of Alcalase, chymotrypsin and pepsin hydrolysates was found to be better than that of glutathione (GSH) (p<0.05). These results show that CPHs have the potential to be used as bioactive ingredients in the formulation of functional foods against oxidative stress.
authors.affiliation: E. H. Graham Centre for Agricultural Innovation, Charles Sturt University, Locked Bag 588, Wagga, NSW 2678, Australia; School of Agricultural and Wine Sciences, Charles Sturt University, Locked Bag 588, Wagga, NSW 2678, Australia; Department of Human Nutritional Sciences, The Richardson Center for Functional Foods and Nutraceuticals, University of Manitoba, Winnipeg R3T 2N2, Canada. Electronic address: aalashi@csu.edu.au.
authors.forename: Adeola M
authors.initials: AM
authors.lastname: Alashi
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Blanchard
authors.forename: Rodney J
authors.initials: RJ
authors.lastname: Mailer
authors.forename: Samson O
authors.initials: SO
authors.lastname: Agboola
authors.forename: A John
authors.initials: AJ
authors.lastname: Mawson
authors.forename: Rong
authors.initials: R
authors.lastname: He
authors.forename: Abraham
authors.initials: A
authors.lastname: Girgih
authors.forename: Rotimi E
authors.initials: RE
authors.lastname: Aluko
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D001315:Australia; D029688:Brassica napus / Q000737:chemistry*; D015227:Lipid Peroxidation; D010940:Plant Proteins / Q000737:chemistry*; D011492:Protein Hydrolysates / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010940:Plant Proteins; D011492:Protein Hydrolysates
keywords: Antioxidant properties; Canola; Membrane ultrafiltration; Protein hydrolysates; Radical scavenging activities; Trolox equivalent antioxidant capacity (TEAC)