title: Combining electrochemical impedance spectroscopy and surface plasmon resonance into one simultaneous read-out system for the detection of surface interactions.
abstract: In this article we describe the integration of impedance spectroscopy (EIS) and surface plasmon resonance (SPR) into one surface analytic device. A polydimethylsiloxane (PDMS) flow cell is created, matching the dimensions of a commercially available sensor chip used for SPR measurements. This flow cell allowed simultaneous measurements between an EIS and a SPR setup. After a successful integration, a proof of principle study was conducted to investigate any signs of interference between the two systems during a measurement. The flow cell was rinsed with 10 mM Tris-HCl and 1× PBS buffer in an alternating manner, while impedance and shifts of the resonance angle were monitored. After achieving a successful proof of principle, a usability test was conducted. It was assessed whether simultaneous detection occurred when: (i) Protein A is adsorbed to the gold surface of the chip; (ii) The non-occupied zone is blocked with BSA molecules and (iii) IgG1 is bound to the Protein A. The results indicate a successful merge between SPR and EIS.
authors.affiliation: Institute for Materials Research IMO, Hasselt University, Wetenschapspark 1, 3590 Diepenbeek, Belgium. thijs.vandenryt@uhasselt.be.
authors.forename: Thijs
authors.initials: T
authors.lastname: Vandenryt
authors.forename: Andrea
authors.initials: A
authors.lastname: Pohl
authors.forename: Bart
authors.initials: B
authors.lastname: van Grinsven
authors.forename: Ronald
authors.initials: R
authors.lastname: Thoelen
authors.forename: Ward
authors.initials: W
authors.lastname: De Ceuninck
authors.forename: Patrick
authors.initials: P
authors.lastname: Wagner
authors.forename: Jörg
authors.initials: J
authors.lastname: Opitz