title: Cell-cycle dependent localization of MELK and its new partner RACK1 in epithelial versus mesenchyme-like cells in Xenopus embryo.
abstract: Maternal Embryonic Leucine zipper Kinase (MELK) was recently shown to be involved in cell division of Xenopus embryo epithelial cells. The cytokinetic furrow of these cells ingresses asymmetrically and is developmentally regulated. Two subpopulations of xMELK, the mMELK (for "mitotic" xMELK) and iMELK ("interphase" xMELK), which differ in their spatial and temporal regulation, are detected in Xenopus embryo. How cells regulate these two xMELK populations is unknown. In this study we show that, in epithelial cells, xMELK is present at a higher concentration at the apical junctional complex, in contrast to mesenchyme-like cells, which have uniform distribution of cortical MELK. Interestingly, mMELK and iMELK also differ by their requirements towards cell-cell contacts to establish their proper cortical localization both in epithelial and mesenchyme-like cells. Receptor for Activated protein Kinase C (RACK1), which we identified as an xMELK partner, co-localizes with xMELK at the tight junction. Moreover, a truncated RACK1 construct interferes with iMELK localization at cell-cell contacts. Collectively, our results suggest that iMELK and RACK1 are present in the same complex and that RACK1 is involved in the specific recruitment of iMELK at the apical junctional complex in epithelial cells of Xenopus embryos.
authors.affiliation: UMR 6290 CNRS Institut de Génétique et Développement de Rennes - Université de Rennes 1, Cell Cycle Group , SFR Biosit, 2 Avenue du Professeur Léon Bernard, CS 34317, 35043 Rennes Cedex , France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Chartrain
authors.forename: Yann
authors.initials: Y
authors.lastname: Le Page
authors.forename: Guillaume
authors.initials: G
authors.lastname: Hatte
authors.forename: Roman
authors.initials: R
authors.lastname: Körner
authors.forename: Jacek Z
authors.initials: JZ
authors.lastname: Kubiak
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Tassan
keywords: Cell division; Cell polarity; Development; Tight junction