title: Expression of bacterial isochorismate synthase (EC 5.4.99.6) in transgenic root cultures ofRubia peregrina.
abstract: The gene coding for isochorismate synthase (EC 5.4.99.6) was amplified by the polymerase chain reaction fromEscherichia coli, cloned into a binary vector. and mobilised intoAgrobacterium rhizogenes LBA9402 which was used to transformRubia peregrina. Transgenic roots containing bacterial isochorismate synthase cDNA expressed twice as much isochorismate synthase activity (4.88 pkat/mg protein) as the control roots (2.45 pkat/mg protein) after 10 days in culture, and accumulatedca. 20% higher levels of total anthraquinones after 30 days in culture. Whilst the amount of total alizarin (free and bound) in the transgenic roots was 30% higher than in control roots, the level of free alizarin wasca. 85% higher.
authors.affiliation: Division of Life Sciences, King's College London, Campden Hill Road, W8 7AH, London, UK.
authors.forename: A H
authors.initials: AH
authors.lastname: Lodhi
authors.forename: R J
authors.initials: RJ
authors.lastname: Bongaerts
authors.forename: R
authors.initials: R
authors.lastname: Verpoorte
authors.forename: S A
authors.initials: SA
authors.lastname: Coomber
authors.forename: B V
authors.initials: BV
authors.lastname: Charlwood