title: Transcriptome interrogation of human myometrium identifies differentially expressed sense-antisense pairs of protein-coding and long non-coding RNA genes in spontaneous labor at term.
abstract: OBJECTIVE
  To identify differentially expressed long non-coding RNA (lncRNA) genes in human myometrium in women with spontaneous labor at term.
  
  
  METHODS
  Myometrium was obtained from women undergoing cesarean deliveries who were not in labor (n = 19) and women in spontaneous labor at term (n = 20). RNA was extracted and profiled using an Illumina® microarray platform. We have used computational approaches to bound the extent of long non-coding RNA representation on this platform, and to identify co-differentially expressed and correlated pairs of long non-coding RNA genes and protein-coding genes sharing the same genomic loci.
  
  
  RESULTS
  We identified co-differential expression and correlation at two genomic loci that contain coding-lncRNA gene pairs: SOCS2-AK054607 and LMCD1-NR_024065 in women in spontaneous labor at term. This co-differential expression and correlation was validated by qRT-PCR, an experimental method completely independent of the microarray analysis. Intriguingly, one of the two lncRNA genes differentially expressed in term labor had a key genomic structure element, a splice site, that lacked evolutionary conservation beyond primates.
  
  
  CONCLUSIONS
  We provide, for the first time, evidence for coordinated differential expression and correlation of cis-encoded antisense lncRNAs and protein-coding genes with known as well as novel roles in pregnancy in the myometrium of women in spontaneous labor at term.
authors.affiliation: Perinatology Research Branch, Program for Perinatal Research and Obstetrics, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH , Bethesda, MD and Detroit, MI , USA .
authors.forename: Roberto
authors.initials: R
authors.lastname: Romero
authors.forename: Adi L
authors.initials: AL
authors.lastname: Tarca
authors.forename: Piya
authors.initials: P
authors.lastname: Chaemsaithong
authors.forename: Jezid
authors.initials: J
authors.lastname: Miranda
authors.forename: Tinnakorn
authors.initials: T
authors.lastname: Chaiworapongsa
authors.forename: Hui
authors.initials: H
authors.lastname: Jia
authors.forename: Sonia S
authors.initials: SS
authors.lastname: Hassan
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Kalita
authors.forename: Juan
authors.initials: J
authors.lastname: Cai
authors.forename: Lami
authors.initials: L
authors.lastname: Yeo
authors.forename: Leonard
authors.initials: L
authors.lastname: Lipovich
mesh_terms: D000328:Adult; D003430:Cross-Sectional Studies; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007231:Infant, Newborn; D007743:Labor, Obstetric / Q000235:genetics* / Q000378:metabolism; D009215:Myometrium / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames / Q000235:genetics*; D011247:Pregnancy; D016372:RNA, Antisense / Q000235:genetics* / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D047929:Term Birth / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome; D055815:Young Adult
chemical_list: D016372:RNA, Antisense; D062085:RNA, Long Noncoding; D012333:RNA, Messenger
keywords: Genomics; lncRNA; parturition; pregnancy; preterm birth