title: The effect of osmotically induced water flows on the permeability and ultrastructure of the rabbit gallbladder.
abstract: The purpose of these experiments was to determine the effect of osmotic gradients on the permeability of the rabbit gallbladder. Increasing the tonicity of the mucosal solution reduced the permeability of the gallbladder to both ions and nonelectrolytes, whereas there was no significant effect when the serosal solution was made hypertonic. These results cannot be explained by solvent/solute interactions in either the epithelial membranes or the unstirred layers. Associated with the changes in permeability was the appearance of the transport number effect and current induced resistance changes. Morphological studies of the gallbladder under these conditions showed that the extracellular spaces of the epithelium and the rest of the wall dilate in the presence of osmotic flow to the serosa, but that the spaces collapse when the flow is in the opposite direction. Reconstruction of the permeability changes from the dimensions of the tissue show that all the physiological phenomena are accounted for by the changes in morphology, the dominant effect being in the lateral intercellular spaces. These results suggest that the lateral spaces are a common pathway shared by all solutes crossing the epithelium, and that diffusion along these spaces becomes rate limiting as the spaces collapse.
authors.affiliation: Department of Physiology, University of California Medical Center, 90024, Los Angeles, California.
authors.forename: A P
authors.initials: AP
authors.lastname: Smulders
authors.forename: J D
authors.initials: JD
authors.lastname: Tormey
authors.forename: E M
authors.initials: EM
authors.lastname: Wright