title: KPC-2 carbapenemase and DHA-1 AmpC determinants carried on the same plasmid in Enterobacter aerogenes.
abstract: This study was conducted to analyse the presence of a plasmid-mediated carbapenem resistance mechanism in a clinical Enterobacter aerogenes isolate from a patient from Jiangsu province, People's Republic of China. PCR and sequencing confirmed that the isolate harboured Klebsiella pneumoniae carbapenemase (KPC)-2, DHA-1 and TEM-1 β-lactamase genes. Both the KPC-2 and DHA-1 genes were transferred to Escherichia coli C600 by transconjugation, and Southern blotting confirmed that these two genes were located on the same plasmid, which was of approximately 56 kb in size. The Enterobacter aerogenes isolate was resistant to carbapenems and other tested antimicrobial agents. The Escherichia coli transconjugant showed reduced susceptibility but not resistance to carbapenems and other β-lactams, indicating the presence of another, possibly permeability-related, resistance mechanism in the clinical isolate.
authors.affiliation: Department of Clinical Laboratory, Wuxi Fifth People's Hospital, Wuxi, Jiangsu 214005, PR China.
authors.forename: Shougang
authors.initials: S
authors.lastname: Kuai
authors.affiliation: Department of Clinical Laboratory, Nanjing Jinling Hospital, Nanjing, Jiangsu 210002, PR China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Shao
authors.affiliation: Department of Clinical Laboratory, Wuxi Fifth People's Hospital, Wuxi, Jiangsu 214005, PR China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Clinical Laboratory, Wuxi Fifth People's Hospital, Wuxi, Jiangsu 214005, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Pei
authors.affiliation: Department of Clinical Laboratory, Wuxi Fifth People's Hospital, Wuxi, Jiangsu 214005, PR China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Department of Clinical Laboratory, Nanjing Jinling Hospital, Nanjing, Jiangsu 210002, PR China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Wuxi Fifth People's Hospital, Wuxi, Jiangsu 214005, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D002681:China; D021902:Enterobacter aerogenes / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D047090:beta-Lactams; C048795:AmpC beta-lactamases; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1; C531581:carbapenemase-2, Klebsiella pneumoniae