title: Predictive correlates of shoot regeneration from potato protoplast culture.
abstract: Adaptation of protoplast regeneration systems for use on untested or recalcitrant potato genotypes can be a time-consuming exercise. Callus growth and xylogenesis were evaluated as early correlates of shooting potential to shorten this process. Callus growth was of limited value for predicting organogenesis but a linear relationship was observed between xylogenesis and shooting frequency. Increases in xylem content above a minimum threshold corresponded with increases in shooting frequency. The predictive value of the relationship was tested using a simple protocol modification (the culture of calli on a filter paper base). Calli on filter paper produced more xylem elements and shoots than those plated directly on medium. The potential of xylem content as a predictive test of shooting frequency is discussed.
authors.affiliation: Scottish Crop Research Institute, DD2 5DA, Invergowrie, Dundee, UK.
authors.forename: S N
authors.initials: SN
authors.lastname: Barr
authors.forename: L A
authors.initials: LA
authors.lastname: Payne
authors.forename: M F
authors.initials: MF
authors.lastname: Dale
authors.forename: M J
authors.initials: MJ
authors.lastname: Wilkinson