title: Quorum sensing activity of Enterobacter asburiae isolated from lettuce leaves.
abstract: Bacterial communication or quorum sensing (QS) is achieved via sensing of QS signaling molecules consisting of oligopeptides in Gram-positive bacteria and N-acyl homoserine lactones (AHL) in most Gram-negative bacteria. In this study, Enterobacteriaceae isolates from Batavia lettuce were screened for AHL production. Enterobacter asburiae, identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was found to produce short chain AHLs. High resolution triple quadrupole liquid chromatography mass spectrometry (LC/MS) analysis of the E. asburiae spent supernatant confirmed the production of N-butanoyl homoserine lactone (C4-HSL) and N-hexanoyl homoserine lactone (C6-HSL). To the best of our knowledge, this is the first report of AHL production by E. asburiae.
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. kokgan@um.edu.my.
authors.forename: Yin Yin
authors.initials: YY
authors.lastname: Lau
authors.forename: Joanita
authors.initials: J
authors.lastname: Sulaiman
authors.forename: Jian Woon
authors.initials: JW
authors.lastname: Chen
authors.forename: Wai-Fong
authors.initials: WF
authors.lastname: Yin
authors.forename: Kok-Gan
authors.initials: KG
authors.lastname: Chan
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism*; D004754:Enterobacter / Q000145:classification* / Q000502:physiology*; D018545:Lactuca / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology*; D053038:Quorum Sensing / Q000502:physiology*; D013045:Species Specificity
chemical_list: D054742:Acyl-Butyrolactones