title: Selection of monosomic addition plants in offspring families using repetitive DNA probes in Beta L.
abstract: The distribution of two repetitive DNA probes Sat-121 and PB6-4, specific for the section Procumbentes of the genus Beta, was tested in 16 B. patellaris monosomic addition families using a dot-blot hybridization procedure. All monosomic additions were accurately distinguished from diploid sib plants with both DNA probes. The probe PB6-4, with the strongest signal after hybridization, was selected for rapid screening of an extensive number of putative monosomic additions in B. patellaris or B. procumbens addition families using a squash-blot hybridization procedure. The probe PB6-4 detected 118 monosomic additions in 640 plants (18.4%) in eight different B. procumbens addition families. The addition family with chromosome 4 of B. procumbens was semi-lethal and could not be tested. The distribution of PB6-4 in B. patellaris addition families was confirmed in 63 addition families using the squash-blot procedure. In 4580 plants of these addition families, 628 individual monosomic additions (13.7%) were found. The relationship of the morphological characteristics of monosomic addition plants to the results of the squash-blot hybridization (plants with signal) using probe PB6-4 is quite rigorous but not complete. The correlation between plants with a signal and chromosome number (2n=19) is complete. These results indicate that sequences present on PB6-4 are probably present on all chromosomes of B. patellaris and B. procumbens. The possibility of utilizing the sequence information of Sat-121 for a PCR-based assay to screen for putative monosomic addition plants was also investigated as an alternative to chromosome counting. The DNA-amplification profiles using the primers REP and REP.INV clearly distinguished monosomic addition plants from their diploid sibs.
authors.affiliation: DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), PO Box 16, NL-6700, AA Wageningen, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Mesbah
authors.forename: T S
authors.initials: TS
authors.lastname: De Bock
authors.forename: J M
authors.initials: JM
authors.lastname: Sandbrink
authors.forename: R M
authors.initials: RM
authors.lastname: Klein-Lankhorst
authors.forename: W
authors.initials: W
authors.lastname: Lange