title: Effect of seasonality on oxidative stress responses and metal accumulation in soft tissues of Aulacomya atra, a mussel from the South Atlantic Patagonian coast.
abstract: This study investigated the effects of pollution and its interaction with temperature on the oxidative status of the ribbed mussel Aulacomya atra in the southern Atlantic Patagonian coast. Animals were collected from four sites with different degree and type of human activity impact, during the summer and winter of 2011. Seawater chromium, copper, manganese, nickel and zinc concentrations were measured, as well as metal accumulation, lipid peroxidation, protein oxidation, reduced glutathione levels, and enzymatic activities of superoxide dismutase and glutathione-S-transferase in gills and digestive glands. Metal bioaccumulation and oxidative stress responses in both tissues were generally higher in mussels from harbor areas. Water temperature had a remarkable effect on gill SOD activity and protein oxidation during winter in mussels from all locations. Methodologically, we conclude that measuring both metal bioaccumulation and oxidative stress responses allowed for a more accurate assessment of the biological effects of metal present in seawater.
authors.affiliation: IQUIBICEN - Departamento Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, Intendente Guiraldes 2160, C1428EHA Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Pablo
authors.initials: P
authors.lastname: Di Salvatore
authors.forename: Javier A
authors.initials: JA
authors.lastname: Calcagno
authors.forename: Nicolás
authors.initials: N
authors.lastname: Ortíz
authors.forename: María Del Carmen
authors.initials: Mdel C
authors.lastname: Ríos de Molina
authors.forename: Sebastián E
authors.initials: SE
authors.lastname: Sabatini
mesh_terms: D000818:Animals; D001118:Argentina; D001267:Atlantic Ocean; D005260:Female; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008670:Metals / Q000493:pharmacokinetics*; D049879:Mytilidae / Q000378:metabolism*; D018384:Oxidative Stress; D012621:Seasons; D012623:Seawater / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000493:pharmacokinetics*
chemical_list: D008670:Metals; D014874:Water Pollutants, Chemical; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005978:Glutathione
keywords: Antioxidant defenses; Aulacomya atra; Metal bioaccumulation; Oxidative stress; Temperature effects; Trace metals