title: Microfluidic chip-based liquid chromatography coupled to mass spectrometry for determination of small molecules in bioanalytical applications: an update.
abstract: Many microfluidic chip-based LC-MS systems have been utilized for high-throughput analysis in various fields of bioanalytical applications such as proteomic, glycomic, pharmaceutical, and clinical research. This review is an update of a previous review article (Electrophoresis 2012, 33, 635-643) to mainly cover the most recent advancements in chip-based LC-MS for determining small molecules in bioanalysis. First, the different types of microfluidic chip devices for chip-based LC-MS analysis will be discussed. Following the discussion of the recent developments in the chip-based instrumentation, the applications of chip-based LC-MS for determining small molecules, such as glycans, pharmaceutical drugs, drugs of abuse, drug metabolites, and biomarkers in various biological sample matrixes will also be included in this review.
authors.affiliation: Department of Chemistry, Soochow University, Taipei, Taiwan.
authors.forename: Shu-Ling
authors.initials: SL
authors.lastname: Lin
authors.forename: Tzuen-Yeuan
authors.initials: TY
authors.lastname: Lin
authors.forename: Ming-Ren
authors.initials: MR
authors.lastname: Fuh
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D004364:Pharmaceutical Preparations / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis
chemical_list: D015415:Biomarkers; D004364:Pharmaceutical Preparations; D011134:Polysaccharides
keywords: Bioanalytical applications; Chip-based LC-MS; MS/MS