title: Chemoenzymatic synthesis of the human CD52 and CD24 antigen analogues.
abstract: Analogs of the human CD52 and CD24 antigens carrying the common core structure of glycosylphosphatidylinositol (GPI) anchors and the intact polypeptide sequences of CD52 and CD24 were chemoenzymatically synthesized. CD52 and CD24 proteins were obtained by solid-phase peptide synthesis and then coupled to chemically synthesized GPI anchors under the influence of a bacterial enzyme, sortase A, to afford the target molecules in good yields.
authors.affiliation: Department of Chemistry, Wayne State University , Detroit, Michigan 48202, United States , and National Glycoengineering Research Center, Shandong University , Jinan 250100, China.
authors.forename: Zhimeng
authors.initials: Z
authors.lastname: Wu
authors.forename: Xueqing
authors.initials: X
authors.lastname: Guo
authors.forename: Guofeng
authors.initials: G
authors.lastname: Gu
authors.forename: Zhongwu
authors.initials: Z
authors.lastname: Guo
mesh_terms: D019881:Aminoacyltransferases / Q000737:chemistry* / Q000378:metabolism; D015703:Antigens, CD / Q000737:chemistry* / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000737:chemistry* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D051927:CD24 Antigen / Q000737:chemistry* / Q000378:metabolism; D000074301:CD52 Antigen; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism; D017261:Glycosylphosphatidylinositols / Q000138:chemical synthesis* / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D015394:Molecular Structure; D060327:Solid-Phase Synthesis Techniques
chemical_list: D015703:Antigens, CD; D000951:Antigens, Neoplasm; D001426:Bacterial Proteins; D051927:CD24 Antigen; D000074301:CD52 Antigen; C000613862:CD52 protein, human; D006023:Glycoproteins; D017261:Glycosylphosphatidylinositols; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases