title: Anti-inflammatory activity of bioaccessible fraction from Eryngium foetidum leaves.
abstract: Eryngium foetidum (EF) has long been used as a medicinal plant and culinary spice in tropical regions. Phytochemicals in its leaves have been proposed to be responsible for the anti-inflammatory and antioxidant activities. The present study used in vitro digestion coupled with Caco-2 cells to assess such activities. Caco-2 cells were incubated with aqueous fraction from simulated digestion (bioaccessible fraction) of EF leaves with/without bile extract prior to stimulation with interleukin-1 beta (IL-1β). Monocyte chemoattractant protein-1 (MCP-1) and IL-8 in culture media and the intracellular reactive oxygen species (ROS) were measured. Approximately 24% β-carotene and 35% lutein of leaves were present in the aqueous fraction. The transfer of caffeic and chlorogenic acids to the aqueous fraction was 76%-81%, while that of kaempferol was 48%. Prior incubation of Caco-2 cells with the bioaccessible fraction suppressed IL-1β activated IL-8 and MCP-1 by 33%, but the fraction lacking mixed micelles decreased IL-8 and MCP-1 levels only by 11%. The pretreatment of Caco-2 cells with the bioaccessible fraction of EF reduced ROS by 34%; the fraction lacking mixed micelles decreased ROS by 28%. These data suggest that bioactive compounds partitioning in mixed micelles play a significant role to suppress the proinflammatory insult but with a modest antioxidant effect.
authors.affiliation: Institute of Nutrition, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand.
authors.forename: Suwitcha
authors.initials: S
authors.lastname: Dawilai
authors.forename: Chawanphat
authors.initials: C
authors.lastname: Muangnoi
authors.forename: Phawachaya
authors.initials: P
authors.lastname: Praengamthanachoti
authors.forename: Siriporn
authors.initials: S
authors.lastname: Tuntipopipat
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D018938:Caco-2 Cells; D018932:Chemokine CCL2 / Q000096:biosynthesis; D031049:Eryngium / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007249:Inflammation / Q000188:drug therapy* / Q000473:pathology; D053583:Interleukin-1beta / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D014975:Lutein / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism; D019207:beta Carotene / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D053583:Interleukin-1beta; D016209:Interleukin-8; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D019207:beta Carotene; D014975:Lutein